<?xml version="1.0" encoding="UTF-8"?>
<uniprot xmlns="https://uniprot.org/uniprot"
 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
 xsi:schemaLocation="http://uniprot.org/uniprot http://www.uniprot.org/docs/uniprot.xsd">
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFN9</accession>
    <name>A0A452GFN9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Spermatogenesis-associated protein 20-like TRX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GFN9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000320"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000363.1">
        <property type="protein sequence ID" value="ENSGAGP00000000320.1"/>
        <property type="gene ID" value="ENSGAGG00000000280.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000000375.1">
        <property type="protein sequence ID" value="ENSGAGP00000000332.1"/>
        <property type="gene ID" value="ENSGAGG00000000280.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02955">
        <property type="entry name" value="SSP411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024705">
        <property type="entry name" value="Ssp411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004879">
        <property type="entry name" value="Ssp411-like_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03190">
        <property type="entry name" value="Thioredox_DsbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 20-like TRX" evidence="1">
        <location>
            <begin position="52"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="862" mass="96591" checksum="62F8CFB5B4C6A258" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GG61</accession>
    <name>A0A452GG61_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GG61"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000569"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000639.1">
        <property type="protein sequence ID" value="ENSGAGP00000000569.1"/>
        <property type="gene ID" value="ENSGAGG00000000472.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF6">
        <property type="entry name" value="BETA-ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="76" mass="8919" checksum="576AEBE5AAF69EA8" modified="2019-05-08" version="1">MSNETTPEAVSSPLVSSPPPQGQRYFDRFSEDDPEYLRIRNMAADLRQDFNMMEQKKRVTMILQSPVPVNLVWLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGQ8</accession>
    <name>A0A452GGQ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the filamin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GGQ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000766"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000865.1">
        <property type="protein sequence ID" value="ENSGAGP00000000766.1"/>
        <property type="gene ID" value="ENSGAGG00000000624.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000092">
        <property type="entry name" value="Filamin-B isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000007">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000079">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000125">
        <property type="entry name" value="filamin-B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000138">
        <property type="entry name" value="filamin-B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000102">
        <property type="entry name" value="filamin-B isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000096">
        <property type="entry name" value="filamin-C isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044801">
        <property type="entry name" value="Filamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537:SF12">
        <property type="entry name" value="FILAMIN-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="51"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="141"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="233"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="323"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="425"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="519"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="616"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="746"/>
            <end position="840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="841" mass="89019" checksum="0CEF4ADCD8142B14" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHE3</accession>
    <name>A0A452GHE3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LRAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the H-rev107 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GHE3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001038"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001170.1">
        <property type="protein sequence ID" value="ENSGAGP00000001038.1"/>
        <property type="gene ID" value="ENSGAGG00000000840.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070292">
        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051496">
        <property type="entry name" value="H-rev107_PLA/AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007053">
        <property type="entry name" value="LRAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13943">
        <property type="entry name" value="HRAS-LIKE SUPPRESSOR - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13943:SF31">
        <property type="entry name" value="PHOSPHOLIPASE A AND ACYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04970">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51934">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="LRAT" evidence="5">
        <location>
            <begin position="14"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="150" mass="16286" checksum="A5132BE25EE81387" modified="2019-05-08" version="1">AAVSGCWTASIPACPAPATGSQLSMAIRCSGYQQGQLEGLTGMPDRGFASSSNGKAIVKSEPLECVVGNCNYRVNNKYDGTRQVYPVPEIIVRANNKVGKTWTYHDFAKNCEHFANEIRYNKARSDQVCNGPGVRYCSLGHVTASRSCFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GI38</accession>
    <name>A0A452GI38_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5-hydroxytryptamine receptor 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Serotonin receptor 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR7 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GI38"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001244"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001397.1">
        <property type="protein sequence ID" value="ENSGAGP00000001244.1"/>
        <property type="gene ID" value="ENSGAGG00000001000.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006939">
        <property type="term" value="P:smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042310">
        <property type="term" value="P:vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15329">
        <property type="entry name" value="7tmA_5-HT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000071">
        <property type="entry name" value="5-hydroxytryptamine (serotonin) receptor 7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001069">
        <property type="entry name" value="5HT_7_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF199">
        <property type="entry name" value="IP13425P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00652">
        <property type="entry name" value="5HT7RECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="144"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="93"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="441" mass="49111" checksum="09BC74F363E3E364" modified="2019-05-08" version="1">MVMDINSSHVSGHLRAFALAAPRELSSSGMVAGSLPPRWLKIIQDVTASPSPASSLAAENDTRCGEQILSYGNMEKVVIGAILSLITLLTIAGNCLVVISVCFVKKLRQPSNYLIVSLALADLSVAVAVMPFVSVTDLIGGEWIFGHVFCNVFIAMDVMCCTASIMTLCVISIDRYLGITRPLTYPVRQNGKCMAKMILCVWLLSASITIPPLFGWAQNVNDEKVCLISQDFGYTIYSTAVAFYIPMSVMLFMYYQIYKAAKRSAAKHKFAGFPRPEENEGMVSVNGVIKLHKESEECTNFSRLLKHDRKNISIFKREQKAATTLGIIVGAFTVCWLPFFILSTARPFICGTACSCIPFWVERTFLWLGYANSLINPFIYAFFNRDLRTTYRNLLQCRYRNINRKLSAAGMHEALKLAEKPEFVLKNCSRDKLLSGKAQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJF8</accession>
    <name>A0A452GJF8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.86</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.86" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452GJF8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001802"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002052.1">
        <property type="protein sequence ID" value="ENSGAGP00000001802.1"/>
        <property type="gene ID" value="ENSGAGG00000001443.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045779">
        <property type="term" value="P:negative regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045659">
        <property type="term" value="P:negative regulation of neutrophil differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045579">
        <property type="term" value="P:positive regulation of B cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050776">
        <property type="term" value="P:regulation of immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09100">
        <property type="entry name" value="INPP5c_SHIP1-INPP5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10343">
        <property type="entry name" value="SH2_SHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000035">
        <property type="entry name" value="phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000005">
        <property type="entry name" value="phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057509">
        <property type="entry name" value="C2_SHIP1-2_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057510">
        <property type="entry name" value="C2_SHIP1-2_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46051:SF3">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 5-PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46051">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24147">
        <property type="entry name" value="C2_SHIP1-2_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24150">
        <property type="entry name" value="C2_SHIP1-2_first"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="14">
        <location>
            <begin position="5"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="913"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="983"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1039"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="933"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="990"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1062"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1122"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1185"/>
            <end position="1194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1214" mass="136870" checksum="86762328CAD6E4B6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GK47</accession>
    <name>A0A452GK47_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MiT/TFE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GK47"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001966"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002237.1">
        <property type="protein sequence ID" value="ENSGAGP00000001966.1"/>
        <property type="gene ID" value="ENSGAGG00000001583.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030318">
        <property type="term" value="P:melanocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18926">
        <property type="entry name" value="bHLHzip_MITF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000003">
        <property type="entry name" value="microphthalmia-associated transcription factor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021802">
        <property type="entry name" value="MiT/TFE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031867">
        <property type="entry name" value="MiT/TFE_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776:SF4">
        <property type="entry name" value="MICROPHTHALMIA-ASSOCIATED TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776">
        <property type="entry name" value="MIP04163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11851">
        <property type="entry name" value="DUF3371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15951">
        <property type="entry name" value="MITF_TFEB_C_3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="11">
        <location>
            <begin position="294"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="471"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="340"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="502" mass="56334" checksum="13F51161657531EA" modified="2019-05-08" version="1">MDALRVQMFMPCSFESFYLSSEHPGASKPPLSSSTMTSRILLRQQLMREQMQEQERREQQQKQQAAQFMQQRVPLSQTPAINVSVPSNLPPPTQVPMEVLKVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHTNQALSLPCPNQPGDHVMPPGTGSSAPNSPMAMLTLNSNCEKEGFYKFEEQSRVESECPALNTHTRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQGMPPSGLNISNSCPANLPNIKRELTACIFPTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRTKELENRQKKLEHANRHLLLRIQELEMQARAHGLSLIPSTGLCSPDMVNRVIKQEPVIDNCNQDVLQRHSDLSCTTTLDLTDGTITFSDNLGTYSVPTKVGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSMSMEDTDHAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKB7</accession>
    <name>A0A452GKB7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GKB7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002147"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002450.1">
        <property type="protein sequence ID" value="ENSGAGP00000002147.1"/>
        <property type="gene ID" value="ENSGAGG00000001735.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044715">
        <property type="term" value="F:8-oxo-dGDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03676">
        <property type="entry name" value="NUDIX_Tnr3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="154" mass="16852" checksum="B6260CD12B1449AB" modified="2019-05-08" version="1">MPEGPPGKYPTCRVLPSLPAVPQAAGGIAAGLGVTETLVKECQEEACIPPSLAAQARPVGTISYTYEDHRGIFPECQFVFDLELPEEFEPHVGDGEMQEFYLLGLDEVKDAITSGDFKPNCALVALDFLIRHGHIQPDHEPHYTELVEGLHQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKT8</accession>
    <name>A0A452GKT8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mo25 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GKT8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002271"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002598.1">
        <property type="protein sequence ID" value="ENSGAGP00000002271.1"/>
        <property type="gene ID" value="ENSGAGG00000001850.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000025">
        <property type="entry name" value="Calcium-binding protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013878">
        <property type="entry name" value="Mo25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182:SF11">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08569">
        <property type="entry name" value="Mo25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="341" mass="39913" checksum="EC64F6829A65AB11" modified="2019-05-08" version="1">MPFPFGKSHKSPADIVKNLKESMAVLEKQDISDKKAEKATEEVSKNLVAMKEILYGTNEKEPQTEAVAQLAQELYNSGLLSTLIADLQLIDFEGKKDVAQIFNNILRRQIGTRTPTVEYICTQQNILFMLLKGYESPEIALNCGIMLRECIRHEPLAKIILWSEQFYDFFRYVEMSTFDIASDAFATFKDLLTRHKLLSAEFLEQHYDRFFSEYEKLLHSENYVTKRQSLKLLGELLLDRHNFTIMTKYISKPENLKLMMNLLRDKSRNIQFEAFHVFKVFVANPNKTQPILDILLKNQTKLIEFLSKFQNDRTEDEQFNDEKTYLVKQIRDLKRPAQQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GM27</accession>
    <name>A0A452GM27_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prostaglandin E2 receptor EP2 subtype</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GM27"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002861"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003274.1">
        <property type="protein sequence ID" value="ENSGAGP00000002861.1"/>
        <property type="gene ID" value="ENSGAGG00000002287.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="8886" checksum="347A776CCA829E3B" modified="2019-05-08" version="1">MNKFMQNEDYKKDLLALRFLAINPIIDPWVFVILRPSVLRVIRSVLCCQMSLKTQENMQTTPAAESKSNKQIDLCGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GM75</accession>
    <name>A0A452GM75_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GM75"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002748"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003144.1">
        <property type="protein sequence ID" value="ENSGAGP00000002748.1"/>
        <property type="gene ID" value="ENSGAGG00000002200.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019362748" evidence="3">
        <location>
            <begin position="28"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="198" mass="21671" checksum="32A05E031C1A67CA" modified="2019-05-08" version="1">MGLYLLRSGVRLHTAALLLAACCGGEAIVQIGFAVGEDHLLSFTATGVVLSCLATVTWLVLKLRQGVLMIALTSAVRTTSLVSLERIKVAWRPYLAYLVGLLGILLARYADQLLPHSGAAQHREVSGAQLAKEEVPVFKRRRRSSSMISPEMSSCSNKTHRRTSLPCIPREQVRSATVLHNPRENPGVRGLRKKEHQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMR1</accession>
    <name>A0A452GMR1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Mediator of RNA polymerase II transcription subunit 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Mediator complex subunit 18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MED18</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 10">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Mediator complex subunit 18 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMR1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003126"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003602.1">
        <property type="protein sequence ID" value="ENSGAGP00000003126.1"/>
        <property type="gene ID" value="ENSGAGG00000002530.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.320.10:FF:000001">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019095">
        <property type="entry name" value="Mediator_Med18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09637">
        <property type="entry name" value="Med18"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="208" mass="23621" checksum="BAE1389733350E76" modified="2019-05-08" version="1">MEAPPVTMMPVTGGTINMMEYLLQGSVLDQSLESLLHRLRGLCDNMEPETFLDHEMVFLLKGQQASPFVLRARCSMDKTGTPWHLRYLGQPEMGDKNRHALVRNCVDIATSENLTDFLMEMGFRMDHEFVAKGHVFRKGIMKIVVYKIFRILVPGNTDSIEPLSLSYLVELSVVAPAGQDVVSDDMRNFAEQLKPLVHLEKIDPKRLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPB3</accession>
    <name>A0A452GPB3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FERM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GPB3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003741"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004329.1">
        <property type="protein sequence ID" value="ENSGAGP00000003741.1"/>
        <property type="gene ID" value="ENSGAGG00000003042.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17102">
        <property type="entry name" value="FERM_F1_FRMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000458">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF5">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="1">
        <location>
            <begin position="22"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="125" mass="14594" checksum="23BC69D23361AF23" modified="2019-05-08" version="1">QRGAGVLSELAYHEQDCARWTGPIQCWWCTGSRFSLVQRDAKGQYLFDLLCHHLNLLEKDYFGIRFVDPDKQRHWLEFTKSVVKQMRAQPPFTMCFRVKFYPTDPAALKEEITSSEMMTLAASEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPG1</accession>
    <name>A0A452GPG1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Leukotriene A(4) hydrolase</fullName>
            <shortName evidence="16">LTA-4 hydrolase</shortName>
            <ecNumber evidence="16">3.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>leukotriene A4 + H2O = leukotriene B4</text>
            <dbReference type="Rhea" id="RHEA:22324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57461"/>
            <dbReference type="ChEBI" id="CHEBI:57463"/>
            <dbReference type="EC" id="3.3.2.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Lipid metabolism; leukotriene B4 biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.6" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A452GPG1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003570"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004116.1">
        <property type="protein sequence ID" value="ENSGAGP00000003570.1"/>
        <property type="gene ID" value="ENSGAGG00000002878.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00878"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004463">
        <property type="term" value="F:leukotriene-A4 hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019370">
        <property type="term" value="P:leukotriene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09599">
        <property type="entry name" value="M1_LTA4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000003">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.320:FF:000002">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000004">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.30:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.320">
        <property type="entry name" value="Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012777">
        <property type="entry name" value="LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049980">
        <property type="entry name" value="LTA4H_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038502">
        <property type="entry name" value="M1_LTA-4_hydro/amino_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034015">
        <property type="entry name" value="M1_LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015211">
        <property type="entry name" value="Peptidase_M1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02411">
        <property type="entry name" value="leuko_A4_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726:SF3">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09127">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01263">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 16" id="KW-0434">Leukotriene biosynthesis</keyword>
    <keyword evidence="7 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 16" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 16" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal" evidence="17">
        <location>
            <begin position="462"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="133"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="265"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="562"/>
            <end position="564"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="614" mass="69690" checksum="0DBC6D7B93A5C021" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQJ5</accession>
    <name>A0A452GQJ5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GQJ5"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004791.1">
        <property type="protein sequence ID" value="ENSGAGP00000004102.1"/>
        <property type="gene ID" value="ENSGAGG00000003372.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039938">
        <property type="entry name" value="Sp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14947">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14947:SF24">
        <property type="entry name" value="ZINC FINGER PROTEIN 781-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="215" mass="23247" checksum="D261228A2621B493" modified="2019-05-08" version="1">MTCEKEEQNYHQGNVEQVDKHRALSQSLKRNVEKIHALSVRKTSFTDQGRGPTNAVSVGRPSIAAQTFPNIGESTQGTGPMNAVIVGKPSLTDQLFLCIRKSTQMRGPMNAVSVGKASLTDQPFLDIRGSTQVKHPMNAVNQPSLSIRESTQGRGPMNAVSVGKASLMDQPFLDIRGSTQVKDPMNAVSVGKPSIAAETLLPIKEFTRGEAVYML</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQT6</accession>
    <name>A0A452GQT6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GQT6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004302"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005055.1">
        <property type="protein sequence ID" value="ENSGAGP00000004302.1"/>
        <property type="gene ID" value="ENSGAGG00000003576.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="61" mass="7181" checksum="7E8427F28E45D50A" modified="2019-05-08" version="1">AATAMAGILAWFWNERFWLPHNVTWADLKNTEEASFPQAEDLYLAFPLAFCIFMIRLVFER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GT79</accession>
    <name>A0A452GT79_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GT79"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005109"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005956.1">
        <property type="protein sequence ID" value="ENSGAGP00000005109.1"/>
        <property type="gene ID" value="ENSGAGG00000004149.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000035">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000041">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001330">
        <property type="entry name" value="Zinc finger protein ZIC 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041643">
        <property type="entry name" value="Znf_ZIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF8">
        <property type="entry name" value="GLIS FAMILY ZINC FINGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18366">
        <property type="entry name" value="zf_ZIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="318"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="348"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="378"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="112"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="412"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="466" mass="50169" checksum="09AD9C2848B0E2D1" modified="2019-05-08" version="1">MAMLLDGGPQFPALGVGGFGAPRHHEGPGRDPGAGMGLGPFGDASHAAAFKLSPAAHELSSGQSSAFTPQGSGYANALGHHHHHHHHAAQVPAYGGAAAAFNSTRDFLFRQRGSGLGEPASSGAQHSIFGGSPGSLHGPPGITESPGYLLFPGLHEQSPSHPSPGGHVENGQMHLGLRGDLFGRPDPYRAVSSPRTDPYAGAQFHNYNPMNMNMGMNVAAHHHHGPGAFFRYMRQPIKQELSCKWVEESQINRPKKSCDRTFSTMHELVTHVTMEHVGGPEQNNHICYWEECPREGKSFKAKYKLVNHIRVHTGEKPFPCPFPGCGKIFARSENLKIHKRTHTGEKPFKCEFEGCDRRFANSSDRKKHMHVHTSDKPYICKVCDKSYTHPSSLRKHMKGTWGAAVPGTCPSPPPAASLSSRFESASDPLLLPPGRRRKRCAKGEPHGPASGVSGGCFAPKTGAPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTM6</accession>
    <name>A0A452GTM6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dynactin subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the dynactin 150 kDa subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTM6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005400"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006286.1">
        <property type="protein sequence ID" value="ENSGAGP00000005400.1"/>
        <property type="gene ID" value="ENSGAGG00000004372.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000006295.1">
        <property type="protein sequence ID" value="ENSGAGP00000005407.1"/>
        <property type="gene ID" value="ENSGAGG00000004372.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000003">
        <property type="entry name" value="dynactin subunit 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022157">
        <property type="entry name" value="Dynactin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF6">
        <property type="entry name" value="DYNACTIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12455">
        <property type="entry name" value="Dynactin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="11 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0243">Dynein</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="16">
        <location>
            <begin position="49"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="101"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="953"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="154"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1280" mass="142638" checksum="59A183940923EDF8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVB1</accession>
    <name>A0A452GVB1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GVB1"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006683.1">
        <property type="protein sequence ID" value="ENSGAGP00000005733.1"/>
        <property type="gene ID" value="ENSGAGG00000004659.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031390">
        <property type="entry name" value="OFCC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33862">
        <property type="entry name" value="OROFACIAL CLEFT 1 CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33862:SF3">
        <property type="entry name" value="OROFACIAL CLEFT 1 CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15680">
        <property type="entry name" value="OFCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="210" mass="23820" checksum="723CEAC81AC10BFA" modified="2019-05-08" version="1">ISLQKFQQKALKQTKQKKSKSAEFLMVKEERTATEGIENPAFNISSTDLSAYQTSEEKVIRHDKLDSTLAAHQQKLRLQAHAEPRGETSVLIILKLSDSSCVPDLTCAFHVKNNLPLLFQAAHGICEDFSDSVRPISFTKTLDLRKEVEDEGIPPYIEQFEKDVQDDIILLGSFALEQVRVKNCKTISLFEENLKEIDMGFLNSKDRNVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVI1</accession>
    <name>A0A452GVI1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">5'-3' exoribonuclease</fullName>
            <ecNumber evidence="12">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Possesses 5'-&gt;3' exoribonuclease activity. May promote termination of transcription by RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452GVI1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006012"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007003.1">
        <property type="protein sequence ID" value="ENSGAGP00000006012.1"/>
        <property type="gene ID" value="ENSGAGG00000004884.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1050:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000001">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000003">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017151">
        <property type="entry name" value="Xrn2/3/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF41">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037239">
        <property type="entry name" value="Exonuclease_Xrn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="15">
        <location>
            <begin position="326"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="410"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="782"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="918"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="416"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="447"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="471"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="927"/>
            <end position="937"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="958" mass="109579" checksum="429E132E6BF0B4F4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWM5</accession>
    <name>A0A452GWM5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GWM5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006511"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007574.1">
        <property type="protein sequence ID" value="ENSGAGP00000006511.1"/>
        <property type="gene ID" value="ENSGAGG00000005210.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF83">
        <property type="entry name" value="(3R)-3-HYDROXYACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="6">
        <location>
            <begin position="10"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="215" mass="21816" checksum="AF6B4BEF1D86DC29" modified="2019-05-08" version="1">MAAPLRLRATLALVTGGGSGIGRAICTRLAEEGALVAVADQNEAGAAETVRGLPRGESGQEHEAFGVDVSSAQSVGELMARIQARFSTPPRVCVSCAGITMDEFLLKQTEAAFDKVLQVNLKGTFLITQAVARGLVESGAPGGSIINMGSIVGKVGNLGQVSYAASKAGVEGLTKTAAKELARWGWVLQMAGGQPGVGTAGQQGGRLKGGGRGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GY39</accession>
    <name>A0A452GY39_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rho GTPase-activating protein 39</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GY39"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006967"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008074.1">
        <property type="protein sequence ID" value="ENSGAGP00000006967.1"/>
        <property type="gene ID" value="ENSGAGG00000005616.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04389">
        <property type="entry name" value="RhoGAP_KIAA1688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000022">
        <property type="entry name" value="Rho GTPase activating protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000011">
        <property type="entry name" value="Rho GTPase-activating protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.530">
        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876">
        <property type="entry name" value="FI04035P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876:SF6">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 39-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00784">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WW" evidence="9">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="11">
        <location>
            <begin position="623"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="10">
        <location>
            <begin position="821"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="70"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="144"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="214"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="331"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="375"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="483"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="381"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="483"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="512"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="524"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="538"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1014" mass="113667" checksum="FECC173A1BF83D5C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GY80</accession>
    <name>A0A452GY80_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 710</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GY80"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007135"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008258.1">
        <property type="protein sequence ID" value="ENSGAGP00000007135.1"/>
        <property type="gene ID" value="ENSGAGG00000005733.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000008259.1">
        <property type="protein sequence ID" value="ENSGAGP00000007136.1"/>
        <property type="gene ID" value="ENSGAGG00000005733.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000161">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000182">
        <property type="entry name" value="zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000186">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000191">
        <property type="entry name" value="zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000203">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001180">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000451">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000502">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000649">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000684">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF139">
        <property type="entry name" value="PAIR-RULE PROTEIN ODD-PAIRED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="514"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="542"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="570"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="660" mass="74671" checksum="6A6E48EBAC52193A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYL7</accession>
    <name>A0A452GYL7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GYL7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007253"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008386.1">
        <property type="protein sequence ID" value="ENSGAGP00000007253.1"/>
        <property type="gene ID" value="ENSGAGG00000005827.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47822:SF3">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 4-LIKE WD40 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47822">
        <property type="entry name" value="CARBOHYDRATE BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="124"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="179"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="346" mass="37220" checksum="72ACE8802CA17CB1" modified="2019-05-08" version="1">MARRRLVRALLESRSTEREEAAQGPAQLCIRYQVPLSSDPHGIYSLRFAPAGGCLAAGFGNGAVQIVNVATASLHLSLFRGHRTRHAITALAYHPGRPSLLLAVGADGIISLYSMDSETPLATMTEKENEINAMDFCVDGSTYATAGKDRHIRLYDSHTNQLSHILQAPDFMTGDVTPSSGHSRRIFALRFHPEDRHMFLTAGWDDCVKIWDKRMAKEAQRVINGPHICGPGLDIQGNCVLTGSWVPHNALQLWDLRTSQLQKNLLFPGGPTQGQFLYAARFCDQDIVVAGGSGTSGASAIHTGTNQVLGEILLPNKPVQAVAVAPGGRVVAVAGVGGNLHIADLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZ25</accession>
    <name>A0A452GZ25_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Macrophage immunometabolism regulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UNC119-binding protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZ25"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007429"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008580.1">
        <property type="protein sequence ID" value="ENSGAGP00000007429.1"/>
        <property type="gene ID" value="ENSGAGG00000005961.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900016">
        <property type="term" value="P:negative regulation of cytokine production involved in inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010764">
        <property type="term" value="P:negative regulation of fibroblast migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029219">
        <property type="entry name" value="UNC119-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31224:SF2">
        <property type="entry name" value="MACROPHAGE IMMUNOMETABOLISM REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31224">
        <property type="entry name" value="UNC119-BINDING PROTEIN C5ORF30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15435">
        <property type="entry name" value="UNC119_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="207" mass="23336" checksum="AAB226EC1867B28B" modified="2019-05-08" version="1">MEVDINGETRTTIATLPLPIAEVSSTGKVEAEKPRCSSTPCSPMRRTVSGYQILHMDSNYLVGFTTGEELLKLAQKCTGSEENKVEAVPTVRSKQLDSGLTRSSRLYKARSRYYQPYEIPAVNGRRRRRMPSSGDKCTKALPYEPYKALHGPLPLCLLKGKRAHSKSLDYLNLDKMNIKEPADTEVLQYQLQHLTLRGDRMFARNNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZW5</accession>
    <name>A0A452GZW5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Toll-like receptor 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZW5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007821"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009018.1">
        <property type="protein sequence ID" value="ENSGAGP00000007821.1"/>
        <property type="gene ID" value="ENSGAGG00000006232.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046696">
        <property type="term" value="C:lipopolysaccharide receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001530">
        <property type="term" value="F:lipopolysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001875">
        <property type="term" value="F:lipopolysaccharide immune receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032497">
        <property type="term" value="P:detection of lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002755">
        <property type="term" value="P:MyD88-dependent toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034142">
        <property type="term" value="P:toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000006">
        <property type="entry name" value="Toll-like receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017241">
        <property type="entry name" value="Toll-like_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF521">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037595">
        <property type="entry name" value="Toll-like_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 21" id="KW-0391">Immunity</keyword>
    <keyword evidence="18 21" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="6 21" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="16 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019101347" description="Toll-like receptor 4" evidence="23">
        <location>
            <begin position="29"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="638"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="24">
        <location>
            <begin position="685"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR037595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="840" mass="94914" checksum="E10936B1C0C5245D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H024</accession>
    <name>A0A452H024_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SKA complex subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Spindle and kinetochore-associated protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SKA1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H024"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007805"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008997.1">
        <property type="protein sequence ID" value="ENSGAGP00000007805.1"/>
        <property type="gene ID" value="ENSGAGG00000006218.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000940">
        <property type="term" value="C:outer kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031110">
        <property type="term" value="P:regulation of microtubule polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1890">
        <property type="entry name" value="Ska1 microtubule binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009829">
        <property type="entry name" value="SKA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042031">
        <property type="entry name" value="SKA1_MBD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28573">
        <property type="entry name" value="SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28573:SF1">
        <property type="entry name" value="SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07160">
        <property type="entry name" value="SKA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="72"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00047182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="109" mass="12212" checksum="50C738E05C1E6FF0" modified="2019-05-08" version="1">MRGHLTYDQINGVVQDLNKAVVRKYKILHQPMKSMSSAVRNLYHRFLEGETKDTKEAPILWVPPSTAQLLPLPNRCNKLQEGGGGSRNMACSGEEAGKRRGQGGHLWKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H289</accession>
    <name>A0A452H289_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 18">Mevalonate kinase</fullName>
            <shortName evidence="18">MK</shortName>
            <ecNumber evidence="3 18">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the phosphorylation of mevalonate to mevalonate 5-phosphate, a key step in isoprenoid and cholesterol biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="17 18">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="3 18"/>
    <dbReference type="AlphaFoldDB" id="A0A452H289"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008617"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009909.1">
        <property type="protein sequence ID" value="ENSGAGP00000008617.1"/>
        <property type="gene ID" value="ENSGAGG00000006832.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000003">
        <property type="entry name" value="Mevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="18" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="4 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="12 18" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="16 18" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13 18" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 18" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019156104" description="Mevalonate kinase" evidence="19">
        <location>
            <begin position="21"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="20">
        <location>
            <begin position="125"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="21">
        <location>
            <begin position="306"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU363087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="402" mass="43168" checksum="84086E93AFD0035C" modified="2019-05-08" version="1">MLRLGATVNLLLVVLHSCSATSLMSDFLCVQAALAVALNLRTFLQIKPSNNGKVLICLPNIGTSATWETFRLRTLLTLFSDGLDESKSPSPEQMDALKEFAGITTELSATESLGTLAFLYLYLSISAKYGQIPSVEIVAWSELPTGTGLGSSAAYCVCLAAALLTACGAIGCPVEAEKSVVRWPEEEMDLINRWAFRGEQVIHGKPSGVDNSVATWGGVLRFQSGKFTSLKRVPTLRILLTNTKVFRSTKILVASVKDKILKFPAIMNPVLASIDAISHECESVLEAMAESPSQEYYPVLEELFDVNQHHLNVIGAGHSSLDNVCRVTATHGLHSKLTGAGGGGCAITLLRPDIDPLMVEAARQDLNACGFECWETSIGAPGICLHSLSSLKAEVLRIFNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2H8</accession>
    <name>A0A452H2H8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H2H8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008616"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009908.1">
        <property type="protein sequence ID" value="ENSGAGP00000008616.1"/>
        <property type="gene ID" value="ENSGAGG00000006831.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46331">
        <property type="entry name" value="VALACYCLOVIR HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46331:SF2">
        <property type="entry name" value="VALACYCLOVIR HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="70"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="271" mass="29811" checksum="51255B6590B7DA2E" modified="2019-05-08" version="1">MARPGAGRALLRSLRVLSQLPPAARGAPPLGLRGPAAAASASYGTSITSAKIKVNGVPLHYKQTGQGDHAVLLLPGMLGSSQSDFGPQLKSMNKQLFTIVAWDPRGYGQSIPPARDFPPDFFERDARDAVDLMQALKFKNFSMLGWSDGGITALIAAAKYPTLIHKLVVWGANAVVTQEDLKLYNAIRDVSKWSEKIRKQMEEMYGHEYFAKTCEAWVDGISQFADKPDGNICQHLLPHIKCPTFIIHGGKDPLVPQFHAEYLHKHIKESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H592</accession>
    <name>A0A452H592_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">small monomeric GTPase</fullName>
            <ecNumber evidence="2">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452H592"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009674"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011112.1">
        <property type="protein sequence ID" value="ENSGAGP00000009674.1"/>
        <property type="gene ID" value="ENSGAGG00000007636.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04146">
        <property type="entry name" value="RERG_RasL11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000718">
        <property type="entry name" value="Ras-like protein family member 11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051065">
        <property type="entry name" value="Ras-related_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45704">
        <property type="entry name" value="RAS-LIKE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="248" mass="27624" checksum="6F52D80FF468AF96" modified="2019-05-08" version="1">MRLVQNMCTIAEYPQAGSASGDGCSGGSSARPRLIKIAVVGASGVGKTALVVRFLTKRFIGDYERNAGNLYSRQIQIDGEMLAIQVQDTPGIQIHEHGLDCNEQLNRCIRWADAVVIVFSITDYKSYELLSQLHQQIRQLHPGNRVPVVVVANKADLLHIKEVEPQNGLQLANMLGCTFYEVSVSENYNDVFNAFHVLCKEVSKQQITSTPEKRRTSLIPRPKSPNMQDLKRRFKQALSAKVRTVTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7M0</accession>
    <name>A0A452H7M0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H7M0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010745"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012317.1">
        <property type="protein sequence ID" value="ENSGAGP00000010745.1"/>
        <property type="gene ID" value="ENSGAGG00000008221.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000140">
        <property type="entry name" value="Lipid A export ATP-binding/permease protein MsbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF13">
        <property type="entry name" value="ANTIGEN PEPTIDE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01896">
        <property type="entry name" value="TAP1PROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="276"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="242"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="465"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="721" mass="77883" checksum="E58CF0A26A706651" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H815</accession>
    <name>A0A452H815_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Taste receptor type 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the G-protein coupled receptor T2R family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H815"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010691"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012260.1">
        <property type="protein sequence ID" value="ENSGAGP00000010691.1"/>
        <property type="gene ID" value="ENSGAGG00000008342.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033038">
        <property type="term" value="F:bitter taste receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13950">
        <property type="entry name" value="7tm_TAS2R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000055">
        <property type="entry name" value="Taste receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007960">
        <property type="entry name" value="TAS2R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11394">
        <property type="entry name" value="TASTE RECEPTOR TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11394:SF149">
        <property type="entry name" value="TASTE RECEPTOR TYPE 2 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05296">
        <property type="entry name" value="TAS2R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="3 13" id="KW-0919">Taste</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="190"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="322" mass="36282" checksum="81B141403A09C4CA" modified="2019-05-08" version="1">MGSCQLGSQLTASLGFLPPAIEFSAGVLTNGYIVALNCIDWAKSRTLTSYDKIITSLVFSRFCLQFLVTSDNVLSMVYPNIFDRFETLQPSLVIWMFTNQVSLCFASCLSVFYCVKIATFNQSLFSWLKLRLSKLVPWLLLGSILYCLVTTVAFTLFSYSYWVFSHNSTDCISTNGTISDNKDNLLEFTFLIHSIGSIFPLTVFIASTVLLIISLWRHIRKMNLNSDLNPNFRNPSTDTHVRALKSVVSFFIIYNIYYVASTFSIGNLSYLNAEWEIWVVSFISAAYPSVHSIILILGNPKLKLASGKILHSTNCCFKEFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAW4</accession>
    <name>A0A452HAW4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HAW4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011874"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013593.1">
        <property type="protein sequence ID" value="ENSGAGP00000011874.1"/>
        <property type="gene ID" value="ENSGAGG00000008332.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000004">
        <property type="entry name" value="Keratin, type II cytoskeletal 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003054">
        <property type="entry name" value="Keratin_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616:SF19">
        <property type="entry name" value="KERATIN 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16208">
        <property type="entry name" value="Keratin_2_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01276">
        <property type="entry name" value="TYPE2KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="6">
        <location>
            <begin position="138"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="191"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="347"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="471" mass="52123" checksum="6CB72C216068E2A4" modified="2019-05-08" version="1">MAQKTISIKSGGATRNFSASSAVIPGSRTSFSSISVSRGGGGGLGRVSGGGFGSRSFHGLGGSKRISVSGGYRAVRSGYGYGLGYGGAGYRIGGAGYGFSGGFAPGAGGIHEVTVNQNLLAPLNLEIDPTIQKVRKEEKEQIKTLNNKFASFIDKVRFLEQQNKVLETKWCLLQEQKTAKSNIEPMFEAYINNLRRQLESLGSERLRLDGELKNMQDTVEDFRIRYEDEVNKRTAAENEFVVLKKDVDTAYMNKVELEAKVDALTDEMNFLRALYEAELSHFQSQISDTSVVLSMDNSRNLDLDGIIAEVKAQYEDIANRSRAEAESWYQTKYEELQLTAGRHGDDLRNTKIEISEMNRMIQRLHSEIDNVKKQCASLQTAIADAEQRGELALKDAQAKLAELEEALQKTKADMARQLREYQELMNVKLALDIEIATYRKLLEGEENRLAGEGVGAVNICKYSGSQMLDTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDA1</accession>
    <name>A0A452HDA1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ectonucleotide pyrophosphatase/phosphodiesterase 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HDA1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012759"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014625.1">
        <property type="protein sequence ID" value="ENSGAGP00000012759.1"/>
        <property type="gene ID" value="ENSGAGG00000009788.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF63">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="28344" checksum="9E37D3F35B04FE50" modified="2019-05-08" version="1">MEEGGDPDSTGHKHGPESQERKNMIGQVDRAVGYLRRCIKDNGLESTLNLIITSDHGMDTVIKENEIYLHAVQNFSFQDIQFELLDYGPNGLLVPKENKLDQVYQALKNAHPKLHVYKKEEFPKRFHYAKNTRLTPLMMYSDPGYVIHGVNFSSLQRIKVQFNKGEHGFDNEDMNMKTIFRAVGPAFRKGLEVEPFESVNIYALLCNLLGITPEPHDGSLSVMQPMLSGHSDPGAGDQLFSHSAWCSGHLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HE13</accession>
    <name>A0A452HE13_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HE13"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013059"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014958.1">
        <property type="protein sequence ID" value="ENSGAGP00000013059.1"/>
        <property type="gene ID" value="ENSGAGG00000010018.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051495">
        <property type="term" value="P:positive regulation of cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902905">
        <property type="term" value="P:positive regulation of supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11761">
        <property type="entry name" value="SH3_FCHSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11762">
        <property type="entry name" value="SH3_FCHSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000041">
        <property type="entry name" value="F-BAR and double SH3 domains protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000033">
        <property type="entry name" value="FCH and double SH3 domains protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000060">
        <property type="entry name" value="FCH and double SH3 domains protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035460">
        <property type="entry name" value="FCHSD_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF4">
        <property type="entry name" value="F-BAR AND DOUBLE SH3 DOMAINS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5 9 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="13">
        <location>
            <begin position="53"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="511"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="609"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="715"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="415"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="736"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="746"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="755" mass="84079" checksum="60C0448FAA6E78D7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HED6</accession>
    <name>A0A452HED6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HED6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013170"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015078.1">
        <property type="protein sequence ID" value="ENSGAGP00000013170.1"/>
        <property type="gene ID" value="ENSGAGG00000010090.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15006">
        <property type="entry name" value="7tmA_GPR176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043523">
        <property type="entry name" value="GPCR_176_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="222"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="316"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="77"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="492"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="530" mass="59841" checksum="224BC11C06575B83" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEJ1</accession>
    <name>A0A452HEJ1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C-C motif chemokine</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the intercrine beta (chemokine CC) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HEJ1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013242"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015159.1">
        <property type="protein sequence ID" value="ENSGAGP00000013242.1"/>
        <property type="gene ID" value="ENSGAGG00000010144.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061640">
        <property type="term" value="P:cytoskeleton-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00169">
        <property type="entry name" value="Chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000012">
        <property type="entry name" value="C-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000827">
        <property type="entry name" value="Chemokine_CC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009991">
        <property type="entry name" value="DCTN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28360">
        <property type="entry name" value="DYNACTIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28360:SF1">
        <property type="entry name" value="DYNACTIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07426">
        <property type="entry name" value="Dynactin_p22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00472">
        <property type="entry name" value="SMALL_CYTOKINES_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4 9" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="11">
        <location>
            <begin position="103"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="194" mass="21938" checksum="08FB39DEE6496618" modified="2019-05-08" version="1">MVWFIIPSLSFQAADGLVKVQVALGNIASKRERVKILYKKIEDLIKYLDPQYIDRMAVPDAMKLQFILAEEQFILSQVALLEQANNLQLFLDSAHIKGSENQASDCCLSHKTNRIPHRLLSAYRIQSPETGCRLPAIVFITKKNRNLCAPPNAPWALGLMKKLDGKKGQSTVVRTEKQPHAKGAKHRKQRKPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGS4</accession>
    <name>A0A452HGS4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Golgi apparatus protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Golgi outpost</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGS4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014064"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016103.1">
        <property type="protein sequence ID" value="ENSGAGP00000014064.1"/>
        <property type="gene ID" value="ENSGAGG00000010559.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001893">
        <property type="entry name" value="Cys-rich_GLG1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017873">
        <property type="entry name" value="Cys-rich_GLG1_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039728">
        <property type="entry name" value="GLG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11884:SF1">
        <property type="entry name" value="GOLGI APPARATUS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11884">
        <property type="entry name" value="SELECTIN LIGAND RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00839">
        <property type="entry name" value="Cys_rich_FGFR"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51289">
        <property type="entry name" value="GLG1_C_RICH"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0730">Sialic acid</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1040"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="179"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="307"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="368"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="503"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="623"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="690"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="806"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="935"/>
            <end position="995"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1073" mass="123603" checksum="D1CEB595F8BEC47C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJK5</accession>
    <name>A0A452HJK5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL14m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L14, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJK5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015094"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017244.1">
        <property type="protein sequence ID" value="ENSGAGP00000015094.1"/>
        <property type="gene ID" value="ENSGAGG00000011397.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000004">
        <property type="entry name" value="39S ribosomal protein L14, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21037">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L14, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21037:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="89"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="329" mass="34972" checksum="F81C84F4BB2774DC" modified="2019-05-08" version="1">ATRTPTNLLETDTGAGTERAVPTGGTGAHRGRHSHFQAALETGPCSCCCGGKKGGGRREGKGTSHCNAVYARLLTYSSVRHGPAPHSFRWTPQGLQLAPPKQGRQHRTHTREAPQPHTSSASSHCLAQLEERGAGDRERGGVASPCAGGGGRGSGRGYSGAPLQRLMRMRPLTWGDQIIPLSLPAEALPFAHACWGSSRGRGEPAACDITGACSAIQKLTRVRVVDNSALGNTPYHRPPKCIHVYNKTGVGKVGDKILLAIKGEKKKALIVGHRMPGPCMTPRFDSNNVVLIEDNGNPTGTRIKTPIPLILRKREGEFSKVLAIAQHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLB1</accession>
    <name>A0A452HLB1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alpha-2-macroglobulin like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000015710"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017939.1">
        <property type="protein sequence ID" value="ENSGAGP00000015710.1"/>
        <property type="gene ID" value="ENSGAGG00000011842.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF182">
        <property type="entry name" value="ALPHA-2-MACROGLOBULIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="9">
        <location>
            <begin position="452"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="10">
        <location>
            <begin position="745"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="11">
        <location>
            <begin position="1376"/>
            <end position="1463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1468" mass="163805" checksum="DF0636BB4340BDFD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLJ4</accession>
    <name>A0A452HLJ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HLJ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015856"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018117.1">
        <property type="protein sequence ID" value="ENSGAGP00000015856.1"/>
        <property type="gene ID" value="ENSGAGG00000011931.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019731">
        <property type="term" value="P:antibacterial humoral response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00199">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.75.10:FF:000001">
        <property type="entry name" value="Anosmin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.75.10">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036645">
        <property type="entry name" value="Elafin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008197">
        <property type="entry name" value="WAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050514">
        <property type="entry name" value="WAP_four-disulfide_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19441:SF34">
        <property type="entry name" value="WAP FOUR-DISULFIDE CORE DOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19441">
        <property type="entry name" value="WHEY ACDIC PROTEIN WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00095">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00003">
        <property type="entry name" value="4DISULPHCORE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00217">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57256">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51390">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019036510" description="WAP domain-containing protein" evidence="6">
        <location>
            <begin position="26"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="7">
        <location>
            <begin position="28"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="7">
        <location>
            <begin position="75"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="124" mass="13157" checksum="4E552E35F6EB8F48" modified="2019-05-08" version="1">MVKSSSIVLLVGLLALWAELPSASGQNITKKEGVCPDTVVEAANCTEECQADSDCEENLKCCQTGCGWSCQIPNEKHGSCPVVQGGIPLLGLCKNQCKVDSQCQGAMKCCMNGCRKLACVRPNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HM51</accession>
    <name>A0A452HM51_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HM51"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016023"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018306.1">
        <property type="protein sequence ID" value="ENSGAGP00000016023.1"/>
        <property type="gene ID" value="ENSGAGG00000012046.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990070">
        <property type="term" value="C:TRAPPI protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14943">
        <property type="entry name" value="TRAPPC5_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016696">
        <property type="entry name" value="TRAPP-I_su5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902">
        <property type="entry name" value="41-2 PROTEIN ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="121" mass="13646" checksum="F46F60195540E7F1" modified="2019-05-08" version="1">MDSRFTRGKSAILERSLTRPKMEVTVWKALFGKEADKLEQANDDDKTYYIIEKEPLINTYISVPKENSTLNCASFTAGIVEAILTYSGFPAKVTAHWHKGTTLMIKFDESVIARDKALDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HM94</accession>
    <name>A0A452HM94_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone RNA hairpin-binding protein RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SLBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HM94"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016108"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018395.1">
        <property type="protein sequence ID" value="ENSGAGP00000016108.1"/>
        <property type="gene ID" value="ENSGAGG00000012096.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071204">
        <property type="term" value="C:histone pre-mRNA 3'end processing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071207">
        <property type="term" value="F:histone pre-mRNA stem-loop binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006398">
        <property type="term" value="P:mRNA 3'-end processing by stem-loop binding and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1120:FF:000001">
        <property type="entry name" value="Histone RNA hairpin-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1120">
        <property type="entry name" value="Histone RNA hairpin-binding protein RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026502">
        <property type="entry name" value="SLBP1/SLBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029344">
        <property type="entry name" value="SLBP_RNA_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038294">
        <property type="entry name" value="SLBP_RNA_bind_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17408">
        <property type="entry name" value="HISTONE RNA HAIRPIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17408:SF7">
        <property type="entry name" value="HISTONE RNA HAIRPIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15247">
        <property type="entry name" value="SLBP_RNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Histone RNA hairpin-binding protein RNA-binding" evidence="4">
        <location>
            <begin position="132"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="245" mass="27869" checksum="83E39B0BE3089FBF" modified="2019-05-08" version="1">MSFRQRSPHGCGGSESSPQHPARWSQGRKRGADGKCRKQNDSHSSVFDDENKYGPSDNRLDSFTTPEGPGPFSRCSDWGSVVEEDEMRTNVNKEIARYKRKLLINEFARERKSSSGSSDSKESTVTVELETDEVVLMRRQKQINYGKNTIAYDRYIKEVPRCHRVPGVHPRTPNKFKKYSRRSWDQQIRLWKVALHSWDPPAEEGSDLQAITPVNLGEMETESIGSNSTESQASSQDNYVSNILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMP2</accession>
    <name>A0A452HMP2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rho-related BTB domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Regulator of cell proliferation and apoptosis. It likely functions as a substrate-adapter that recruits key substrates, e.g. MSI2, to CUL3-based ubiquitin ligase complexes for degradation. Required for MSI2 ubiquitination and degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with HSP90AA1 and HSP90AB1. Forms a complex with CUL3 and RBX1. Interacts (via BTB 1 domain) with CUL3. Interacts with MSI2.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HMP2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016213"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018509.1">
        <property type="protein sequence ID" value="ENSGAGP00000016213.1"/>
        <property type="gene ID" value="ENSGAGG00000012159.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18531">
        <property type="entry name" value="BACK_RHOBTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18356">
        <property type="entry name" value="BTB1_POZ_RHOBTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18359">
        <property type="entry name" value="BTB2_POZ_RHOBTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01873">
        <property type="entry name" value="RhoBTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000050">
        <property type="entry name" value="Rho related BTB domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000179">
        <property type="entry name" value="Rho-related BTB domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000177">
        <property type="entry name" value="Rho-related BTB domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000014">
        <property type="entry name" value="Rho-related BTB domain-containing protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="10">
        <location>
            <begin position="361"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="10">
        <location>
            <begin position="598"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="402"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="449"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="804"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="449"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00074749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="834" mass="94595" checksum="C9BACE9C01E1587F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HP99</accession>
    <name>A0A452HP99_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NACHT and WD repeat domain-containing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Leucine-rich repeat and WD repeat-containing protein KIAA1239</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000016799"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019176.1">
        <property type="protein sequence ID" value="ENSGAGP00000016799.1"/>
        <property type="gene ID" value="ENSGAGG00000012551.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001157">
        <property type="entry name" value="Leucine-rich repeat and WD repeat-containing protein KIAA1239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000299">
        <property type="entry name" value="NACHT and WD repeat domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000727">
        <property type="entry name" value="NACHT and WD repeat domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000926">
        <property type="entry name" value="NACHT and WD repeat domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056534">
        <property type="entry name" value="Beta-prop_NWD2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052752">
        <property type="entry name" value="NACHT-WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057588">
        <property type="entry name" value="NWD1/2-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871">
        <property type="entry name" value="BETA TRANSDUCIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871:SF39">
        <property type="entry name" value="NACHT AND WD REPEAT DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23586">
        <property type="entry name" value="Beta-prop_NWD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25469">
        <property type="entry name" value="WHD_NWD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="7">
        <location>
            <begin position="411"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="NWD1/2-like winged helix-turn-helix" evidence="8">
        <location>
            <begin position="637"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="NWD2 C-terminal beta-propeller" evidence="6">
        <location>
            <begin position="1314"/>
            <end position="1644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00081648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1743" mass="197806" checksum="55EEC22F7C565580" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQ83</accession>
    <name>A0A452HQ83_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Kinesin-like protein KIF21A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Processive microtubule plus-end directed motor protein involved in neuronal axon guidance. Is recruited by KANK1 to cortical microtubule stabilizing complexes (CMSCs) at focal adhesions (FAs) rims where it promotes microtubule capture and stability. Controls microtubule polymerization rate at axonal growth cones and suppresses microtubule growth without inducing microtubule disassembly once it reaches the cell cortex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000017191"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019617.1">
        <property type="protein sequence ID" value="ENSGAGP00000017191.1"/>
        <property type="gene ID" value="ENSGAGG00000012827.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01372">
        <property type="entry name" value="KISc_KIF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22263">
        <property type="entry name" value="Rcc_KIF21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000233">
        <property type="entry name" value="Kinesin family member 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000370">
        <property type="entry name" value="Kinesin family member 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000011">
        <property type="entry name" value="Kinesin family member 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056533">
        <property type="entry name" value="KIF21A/B_hel_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056532">
        <property type="entry name" value="KIF21A/B_hel_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF31">
        <property type="entry name" value="KINESIN FAMILY MEMBER 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23203">
        <property type="entry name" value="KIF21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23204">
        <property type="entry name" value="KIF21A_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25764">
        <property type="entry name" value="KIF21A_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14 22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="15 21" id="KW-0505">Motor protein</keyword>
    <keyword evidence="11 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 20" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Kinesin motor" evidence="24">
        <location>
            <begin position="9"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="20">
        <location>
            <begin position="1333"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="20">
        <location>
            <begin position="1570"/>
            <end position="1611"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="20">
        <location>
            <begin position="1612"/>
            <end position="1645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="554"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="839"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1108"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1182"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="379"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="645"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="938"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="560"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="599"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1238"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1282"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="88"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00073302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1664" mass="186718" checksum="DB1F95CC2BB7D080" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HR47</accession>
    <name>A0A452HR47_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Phosphatidylserine synthase</fullName>
            <ecNumber evidence="23">2.7.8.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Serine-exchange enzyme</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) is replaced by L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1-(1Z-octadecenyl)-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphoethanolamine + L-serine = 1-(1Z-octadecenyl)-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phospho-L-serine + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:41496"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:78263"/>
            <dbReference type="ChEBI" id="CHEBI:78264"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:41497"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1-(1Z-octadecenyl)-2-(5Z,8Z,11Z,14Z- eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + L-serine = 1-(1Z-octadecenyl)-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-L-serine + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:41604"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:78342"/>
            <dbReference type="ChEBI" id="CHEBI:78343"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:41605"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1-(1Z-octadecenyl)-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + L-serine = 1-(1Z-octadecenyl)-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:41600"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:78340"/>
            <dbReference type="ChEBI" id="CHEBI:78341"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:41601"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphoethanolamine + L-serine = 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphoserine + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:41488"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:78261"/>
            <dbReference type="ChEBI" id="CHEBI:78262"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:41489"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + L-serine = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:41484"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:73007"/>
            <dbReference type="ChEBI" id="CHEBI:75029"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:41485"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphoethanolamine + L-serine = 1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphoserine + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:41492"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:78265"/>
            <dbReference type="ChEBI" id="CHEBI:78266"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:41493"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine + L-serine = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphoserine + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:41500"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:78268"/>
            <dbReference type="ChEBI" id="CHEBI:78269"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:41501"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + L-serine = 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:40795"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:75038"/>
            <dbReference type="ChEBI" id="CHEBI:78260"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:40796"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + L-serine = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:27606"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="2.7.8.29"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:27607"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 23">Phospholipid metabolism; phosphatidylserine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 23">Endoplasmic reticulum membrane</location>
            <topology evidence="1 23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 23">Belongs to the phosphatidyl serine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.29" evidence="23"/>
    <dbReference type="AlphaFoldDB" id="A0A452HR47"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017491"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019947.1">
        <property type="protein sequence ID" value="ENSGAGP00000017491.1"/>
        <property type="gene ID" value="ENSGAGG00000012990.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00948"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106245">
        <property type="term" value="F:L-serine-phosphatidylethanolamine phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006659">
        <property type="term" value="P:phosphatidylserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004277">
        <property type="entry name" value="PSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF7">
        <property type="entry name" value="PHOSPHATIDYLSERINE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03034">
        <property type="entry name" value="PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 23" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 23" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 23" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 23" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="13 23" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 23" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="353"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="423"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00035875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00036644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00036733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU368094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="452" mass="53460" checksum="B4D7C989AF9F3280" modified="2019-05-08" version="1">MLVFCNDSPHLPCPPQTSHYDLLLIDFFLNCRRAHTLTVLFILTCALGYVTLLEETPQDTAYNTKRGIVASILVFLCFGVTQAKDGPFSRPHPAYWRFWLCVSVVYELFLIFILFQTVHDGRQFMKYIDPNLGVALPERDYGGNCLIYDPGNKTDPFHNVWDKLDGFVPAHFLGWYLKTLMIRDWWMCMIISVMFEFLEYSLEHQLPNFSECWWDHWIMDVILCNGFGIYCGMKTLGWLSLKTYKWQGLWNIPTYKGKIKRIAFQFTPYSWVKFEWKPASSLRRWLAVCGIIFVFLLAELNTFYLKFVLWMPPEHYLVLLRLVFFVNVGGVGMREIYDFMDDPKFHKKLGQQAWLVAAITVTEFLIVVKYDPYTLMLSLPFYITQCWILGIMLVLTWTIWRFFIRDITLRCKEIRRQQQEHKYEKDKCLSNGDGHSPVPDEQNGNKLRERKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HR87</accession>
    <name>A0A452HR87_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HR87"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017568"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020041.1">
        <property type="protein sequence ID" value="ENSGAGP00000017568.1"/>
        <property type="gene ID" value="ENSGAGG00000013051.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22977">
        <property type="entry name" value="DD_FBXL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22124">
        <property type="entry name" value="F-box_FBXL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000134">
        <property type="entry name" value="F-box and leucine rich repeat protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000291">
        <property type="entry name" value="F-box and leucine rich repeat protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF275">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="229"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="751" mass="86004" checksum="01F75B2722697C9A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSR0</accession>
    <name>A0A452HSR0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sulfotransferase</fullName>
            <ecNumber evidence="9">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Atypical sulfotransferase family member with very low affinity for 3'-phospho-5'-adenylyl sulfate (PAPS) and very low catalytic activity towards L-triiodothyronine, thyroxine, estrone, p-nitrophenol, 2-naphthylamine, and 2-beta-naphthol. May have a role in the metabolism of drugs and neurotransmitters in the CNS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452HSR0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018124"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020661.1">
        <property type="protein sequence ID" value="ENSGAGP00000018124.1"/>
        <property type="gene ID" value="ENSGAGG00000013426.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001121">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="10">
        <location>
            <begin position="46"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="284" mass="33067" checksum="DB061487F89EC75B" modified="2019-05-08" version="1">MAESEAETPSTPGEFESKYFEYNGVRLPPFCRGKMEEIANFPVRGSDVWIVTYPKSGTGLLQEVVYLVSQGADPDEIGLMNIDEQLPVLEYPQPGLDIIKELTSPRLIKSHLPYRFLPSDLHNGDSKVIYMARNPKDLVVSYYQFHRSLRTMSYRGTFQEFCRRFMNDKLGYGSWFEHVQEFWEHHMDSNVLFLKYEDMHKDLATMVEQLVRFLGVSYDKAQLESMVEHCHQLIDQCCNAEALPVGRGRVGLWKDIFTVSMNEKFDLVYKQKMGKCDLTFDFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HT81</accession>
    <name>A0A452HT81_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ODF2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HT81"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018306"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020861.1">
        <property type="protein sequence ID" value="ENSGAGP00000018306.1"/>
        <property type="gene ID" value="ENSGAGG00000013546.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902018">
        <property type="term" value="P:negative regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026099">
        <property type="entry name" value="Odf2-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23162">
        <property type="entry name" value="OUTER DENSE FIBER OF SPERM TAILS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23162:SF7">
        <property type="entry name" value="PROTEIN BCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="98"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="214"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="413"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="556" mass="65264" checksum="60093F9DC7010205" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTC9</accession>
    <name>A0A452HTC9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIAS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HTC9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018345"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020908.1">
        <property type="protein sequence ID" value="ENSGAGP00000018345.1"/>
        <property type="gene ID" value="ENSGAGG00000013585.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16819">
        <property type="entry name" value="SP-RING_PIAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000001">
        <property type="entry name" value="E3 SUMO-protein ligase PIAS2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.780:FF:000001">
        <property type="entry name" value="E3 SUMO-protein ligase PIAS2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000003">
        <property type="entry name" value="E3 SUMO-protein ligase PIAS2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.780">
        <property type="entry name" value="PINIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023321">
        <property type="entry name" value="PINIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038654">
        <property type="entry name" value="PINIT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF12">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE PIAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14324">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51466">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAP" evidence="15">
        <location>
            <begin position="2"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="PINIT" evidence="17">
        <location>
            <begin position="125"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="16">
        <location>
            <begin position="322"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="651" mass="72847" checksum="17FD35D4E27DC8C1" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTF9</accession>
    <name>A0A452HTF9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BLOC-1-related complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BORCS5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HTF9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018378"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020946.1">
        <property type="protein sequence ID" value="ENSGAGP00000018378.1"/>
        <property type="gene ID" value="ENSGAGG00000013606.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098574">
        <property type="term" value="C:cytoplasmic side of lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072384">
        <property type="term" value="P:organelle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903744">
        <property type="term" value="P:positive regulation of anterograde synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22789">
        <property type="entry name" value="BORCS5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018780">
        <property type="entry name" value="TBORCS5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31634">
        <property type="entry name" value="BLOC-1-RELATED COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31634:SF2">
        <property type="entry name" value="BLOC-1-RELATED COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10158">
        <property type="entry name" value="LOH1CR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="123" mass="13456" checksum="A618973F594556A9" modified="2019-05-08" version="1">MGAEQSAEAENRPNDLSTSASPSPAKQRAKMDDIVVVAPGTQSSRNVSNDPDVIKLQEIPTFQPLLTGLLSGQTSPTNSKLEKLDSQQVLQLCLRYQDHLHQCAEAVAFDQNALVKRIKEQDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUT8</accession>
    <name>A0A452HUT8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anaphase-promoting complex subunit 4-like WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HUT8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018842"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021479.1">
        <property type="protein sequence ID" value="ENSGAGP00000018842.1"/>
        <property type="gene ID" value="ENSGAGG00000013944.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053053">
        <property type="entry name" value="WD_repeat_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44566">
        <property type="entry name" value="TRANSDUCIN/WD40 REPEAT-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44566:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="233"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="2">
        <location>
            <begin position="450"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="535" mass="60359" checksum="97DC805C5A2A98B6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVE7</accession>
    <name>A0A452HVE7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HVE7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019116"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021784.1">
        <property type="protein sequence ID" value="ENSGAGP00000019116.1"/>
        <property type="gene ID" value="ENSGAGG00000014138.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017081">
        <property type="term" value="F:chloride channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006821">
        <property type="term" value="P:chloride transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029181">
        <property type="entry name" value="Barttin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28399">
        <property type="entry name" value="BARTTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28399:SF1">
        <property type="entry name" value="BARTTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15462">
        <property type="entry name" value="Barttin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="299" mass="32487" checksum="652DEB2FAAA69AE1" modified="2019-05-08" version="1">MAEDKTFRYGLIVLGFFLVMIGMFIMSVDKPQVYITFCTLGVLTIAVGITWSMCQCYPKVGPSLLSVLSHSASLARGGYVQGKNRPQVLIDNANVYEKSLPSYEQIQRKAKGSVECLGIQMAPLLGDCELKPRGCPHSFVQAKAEVHRISENNGEMFRDPASQMVTAAISSAAPLASFLDDTDFPSSEGSTSSSLNPLPSQGHTQKPRSELPRYEDFALIDSPLAEGWHPSQEQTLALPQNYLSGAASARQFALVSGPGSKAAESGERQSMGEDDMYYGLKEGPENLLIADDFVFEPET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVU4</accession>
    <name>A0A452HVU4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HVU4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019240"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021918.1">
        <property type="protein sequence ID" value="ENSGAGP00000019240.1"/>
        <property type="gene ID" value="ENSGAGG00000014220.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19750">
        <property type="entry name" value="bHLH-O_DEC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000020">
        <property type="entry name" value="class E basic helix-loop-helix protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="44"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="13">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="182"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="195"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="450" mass="48143" checksum="E8361CEC890BE939" modified="2019-05-08" version="1">MDEGIPRLQDRQLLEHVDFIGLDYPSLYLCKPKRGMKRDESKETYKLPHRLIEKKRRDRINECIAQLKDLLPEHLKLTTLGHLEKAVVLELTLKHLKALTALTEQQHQNIIALQNGERSVKSPIQCDLDAFHSGFQTCAKEVLQYLSRFESWTPREQRCAQLVNHLHAVSTQFLPSPQLLTPQVPVSKGSSSSSCAQDRTGQKLEAQTNCVPVIQRTHPPVELGGENDTDTDSGYGGESEGRPDREKGQAAGLPGVTIKQEPAGDEAPAPKRLKLDCSSATLPAAAAATLSPDHQAAAALLRPDAALLSSLMAFSGGGAAFGPQAAAAPLCLPFYFISPSAAAAYMQPLLDKSHLEKYLYPAAAPIPLLYSGIPAQTAAAFPCLSSVLTPADKANAAAAAAAASALLPLDVVSAGHHLPHLFAAACETGPSLDSDLPSPEDLLQPGKESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HX44</accession>
    <name>A0A452HX44_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ephrin RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the ephrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HX44"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019783"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022539.1">
        <property type="protein sequence ID" value="ENSGAGP00000019783.1"/>
        <property type="gene ID" value="ENSGAGG00000014585.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10426">
        <property type="entry name" value="Ephrin-B_Ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000020">
        <property type="entry name" value="Ephrin-B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031328">
        <property type="entry name" value="Ephrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034255">
        <property type="entry name" value="Ephrin-B_Ecto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019765">
        <property type="entry name" value="Ephrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001799">
        <property type="entry name" value="Ephrin_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304">
        <property type="entry name" value="EPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304:SF18">
        <property type="entry name" value="EPHRIN-B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00812">
        <property type="entry name" value="Ephrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01347">
        <property type="entry name" value="EPHRIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01299">
        <property type="entry name" value="EPHRIN_RBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51551">
        <property type="entry name" value="EPHRIN_RBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Ephrin RBD" evidence="18">
        <location>
            <begin position="30"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="166"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="91"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="333" mass="36770" checksum="DD2369778B7250A4" modified="2019-05-08" version="1">MAVRRGDSSLWKYGWGVLMVLCRTAMARSIVLDPIYWNSSNPKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADSCTIKKDNTPLLNCAKPDQDVKFTIKFQEFSPNLWGLEFQRKKDYYIISTSNGSLEGLDNQEGGVCQTKAMKILMKVGQDPNSAGSSRNTDPTKRPEQEAGTNGKSSTTSPFVKDNSGSNTDGSKAGHSSILGSEVALFAGIASGCIIFIVIIITLVVLLLKYRRRHRKHSPQHATTLSLSTLATPKRSGNNNGSEPSDIIIPLRTADSVFCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXK1</accession>
    <name>A0A452HXK1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HPS5-like beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000019867"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022633.1">
        <property type="protein sequence ID" value="ENSGAGP00000019867.1"/>
        <property type="gene ID" value="ENSGAGG00000014338.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032527">
        <property type="term" value="P:protein exit from endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056499">
        <property type="entry name" value="Beta-prop_HPS5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006624">
        <property type="entry name" value="Beta-propeller_rpt_TECPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23287">
        <property type="entry name" value="RUBY-EYE2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23287:SF16">
        <property type="entry name" value="TECTONIN BETA-PROPELLER REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23756">
        <property type="entry name" value="Beta-prop_HPS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06462">
        <property type="entry name" value="Hyd_WA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19193">
        <property type="entry name" value="Tectonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00706">
        <property type="entry name" value="TECPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPS5-like beta-propeller" evidence="2">
        <location>
            <begin position="36"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="741"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1414" mass="156187" checksum="BD38F17A517E5F54" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I153</accession>
    <name>A0A452I153_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I153"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024177.1">
        <property type="protein sequence ID" value="ENSGAGP00000021229.1"/>
        <property type="gene ID" value="ENSGAGG00000015592.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="125" mass="14298" checksum="0A4628F4C0DF08FF" modified="2019-05-08" version="1">MGGLQKTALGMQWVMCPCISPSFALSLCQLLWVSITPQSNWMFRKDYYNQTVKRRLLMSPVFNHHTVEWFYAYFICISLNRVSVGHSEPGKVQCCGGRDRKVPLMPIFKSQGLSMCAMLPTAPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1L6</accession>
    <name>A0A452I1L6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the actin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I1L6"/>
    <dbReference type="SMR" id="A0A452I1L6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021378"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024350.1">
        <property type="protein sequence ID" value="ENSGAGP00000021378.1"/>
        <property type="gene ID" value="ENSGAGG00000015696.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000024355.1">
        <property type="protein sequence ID" value="ENSGAGP00000021383.1"/>
        <property type="gene ID" value="ENSGAGG00000015696.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.36.70:FF:000001">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000131">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000047">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0558">Oxidation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="376" mass="41877" checksum="6EC08CD5EEAD445E" modified="2019-05-08" version="1">MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKPEYDEAGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1S9</accession>
    <name>A0A452I1S9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Centromere protein U</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">MLF1-interacting protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-U/AME1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I1S9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021434"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024416.1">
        <property type="protein sequence ID" value="ENSGAGP00000021434.1"/>
        <property type="gene ID" value="ENSGAGG00000015743.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025214">
        <property type="entry name" value="CENP-U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32222">
        <property type="entry name" value="CENTROMERE PROTEIN U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32222:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13097">
        <property type="entry name" value="CENP-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="68"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="284"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="424" mass="48439" checksum="69966B69F143774A" modified="2019-05-08" version="1">MDKKRKMHGKSHMPALEKTSKKKLKRNKLDLDVEDVALIPTQEYKAKSHPRGKLLPCEEPDVSRILKITETGQVEEPDDSFDHPLHSTAVDAGGNVELQEEEHDMSASHSSDSFPQEKNAEARKNQCSLKMPEDNKSETEDSEDESLKENKIQKKISGNAKTNVEESKLPSALTLRNSSSESSISNKVTDLVKKLNAQKKPRNSAKSHVKPKKSSQKSALEPSADDSPFSIQIWCPKGLRRFSQDITELDVVLAENEKITADYKQRVESRICREAIAVFYSGFKEQLTNTITEVQELKDLKRKNAKVITDINKKRRRLLEVREELIRTGPQLKQLQREYAELQERESSLRNATQFLTDLKEMQQQYINCREDHPQEKVVYGTSSIPALLVESQRILRAESHFQNISTRLQECLNLQKKELPGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3D4</accession>
    <name>A0A452I3D4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Osteocalcin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Binds strongly to apatite and calcium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="11 12">Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the osteocalcin/matrix Gla protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3D4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022008"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025073.1">
        <property type="protein sequence ID" value="ENSGAGP00000022008.1"/>
        <property type="gene ID" value="ENSGAGG00000016143.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046848">
        <property type="term" value="F:hydroxyapatite binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008147">
        <property type="term" value="F:structural constituent of bone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031214">
        <property type="term" value="P:biomineral tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060348">
        <property type="term" value="P:bone development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001649">
        <property type="term" value="P:osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030500">
        <property type="term" value="P:regulation of bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900076">
        <property type="term" value="P:regulation of cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032571">
        <property type="term" value="P:response to vitamin K"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058704">
        <property type="entry name" value="BGLAP-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039176">
        <property type="entry name" value="Osteocalcin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002384">
        <property type="entry name" value="Osteocalcin/MGP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14235">
        <property type="entry name" value="OSTEOCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14235:SF0">
        <property type="entry name" value="OSTEOCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25890">
        <property type="entry name" value="BGLAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00002">
        <property type="entry name" value="GLABONE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0091">Biomineralization</keyword>
    <keyword evidence="7 9 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 11 12" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="6 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Gla" evidence="14">
        <location>
            <begin position="59"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="4-carboxyglutamate" evidence="11">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-carboxyglutamate" evidence="11">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-carboxyglutamate" evidence="11">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="67"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR602384-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602384-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602384-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="93" mass="10188" checksum="687CE699B662A0C5" modified="2019-05-08" version="1">ALRLGSPPKPCRAATGQSRYGNAEPSGAILLTPPLLSALAAFVSKQDSPRAFYDLIHDPLEGKREICELNPDCDELADHIGFQEAYRRYYGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3P2</accession>
    <name>A0A452I3P2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Decorin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Bone proteoglycan II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 16">May affect the rate of fibrils formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Binds to type I and type II collagen, fibronectin and TGF-beta. Forms a ternary complex with MFAP2 and ELN.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Binds to type I and type II collagen, to fibronectin and TGF-beta. Forms a ternary complex with MFAP2 and ELN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14 16">Secreted</location>
            <location evidence="2 14 16">Extracellular space</location>
            <location evidence="2 14 16">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14 16">Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3P2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022136"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025232.1">
        <property type="protein sequence ID" value="ENSGAGP00000022136.1"/>
        <property type="gene ID" value="ENSGAGG00000016258.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000025235.1">
        <property type="protein sequence ID" value="ENSGAGP00000022139.1"/>
        <property type="gene ID" value="ENSGAGG00000016258.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000038">
        <property type="entry name" value="Biglycan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016352">
        <property type="entry name" value="SLRP_I_decor/aspor/byglycan"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF14">
        <property type="entry name" value="DECORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002490">
        <property type="entry name" value="SLRP_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14 16" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 14 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14 16">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044516216" description="Decorin" evidence="14 16">
        <location>
            <begin position="17"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="17">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="51"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="310"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR002490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR002490-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU364097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="356" mass="39530" checksum="823C383A92BE3976" modified="2019-05-08" version="1">MKVAVFFLLLLPVCWAKPFHQKGLFEFMLEDEGSADLPTVSPDIYLPGAVCPFRCQCHLRVVQCSDLGLDKVPRDLPSDTTLLDLQNNKITEIKDGDFKNLKDLHALILVNNKISKISPAAFAPLKKLERLYLSKNNLKELPENMPKSLQELRAHENEITKLRKAVFNGLNSMIVVELGTNPLKSSGVENGAFQGMKKLSYIRISDTNITSIPRGLPPSLTELHLDGNKITKIDSESLFGLANLAKLGLSFNSISAVDNGSLANIPHLKELHLDNNELVKVPGGLAEHKYIQVVYLHNNKIASVGPSDFCPPGYNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYERSAIQLGNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4B3</accession>
    <name>A0A452I4B3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I4B3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022398"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025521.1">
        <property type="protein sequence ID" value="ENSGAGP00000022398.1"/>
        <property type="gene ID" value="ENSGAGG00000016435.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18290">
        <property type="entry name" value="BTB_POZ_BTBD14B_NAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2590:FF:000002">
        <property type="entry name" value="Putative nucleus accumbens-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000009">
        <property type="entry name" value="Zinc finger and BTB domain-containing 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2590">
        <property type="entry name" value="BEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018379">
        <property type="entry name" value="BEN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF3">
        <property type="entry name" value="NUCLEUS ACCUMBENS-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10523">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01025">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51457">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="30"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="BEN" evidence="7">
        <location>
            <begin position="368"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="187"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="138"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="271"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="521" mass="56796" checksum="45BF74D31177C8D8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4Q9</accession>
    <name>A0A452I4Q9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I4Q9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022553"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025695.1">
        <property type="protein sequence ID" value="ENSGAGP00000022553.1"/>
        <property type="gene ID" value="ENSGAGG00000016545.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15095">
        <property type="entry name" value="7tmA_KiSS1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000171">
        <property type="entry name" value="KISS1 receptor b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008103">
        <property type="entry name" value="KiSS_1_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF23">
        <property type="entry name" value="GALANIN-LIKE G-PROTEIN COUPLED RECEPTOR NPR-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01728">
        <property type="entry name" value="KISS1RECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="81"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="389" mass="44051" checksum="6DCA8B109BB9988A" modified="2019-05-08" version="1">MAGSPRMDAEPSPLGGWLDNGSWTPVPTAAPHLEISTPVVPGRRGLWIFNSSGEDTSPPFLTDAWLVPLFYALVMLLGLVGNSLVIYVVSKHRQMRTATNFYIANLATTDIIFLVCCVPFTATLYPLPSWIFGDFMCKLVNYLQQVTAQATCITLTAMSMDRCYATLYPLQSLRYRTPRVAMGVSVAIWISSFLLSLPIAMYHRTEVGYWYGLRTYCIEAFASKSQERSIILYTFLAVYLLPLLTICLCYSIMLKRMGRPIVEPIDHNYQQVQHLSERSVAMRAKVSKMVVVIVVLFTVCWGPIQLYLLFQGFYGSFQASYETYKIKTWANCMSYANSSINPIVYAFMGDSFRKSFKKAFPFLFRHRVRDGAVLSGSRNAKMKFVTEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5Z7</accession>
    <name>A0A452I5Z7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I5Z7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023023"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026237.1">
        <property type="protein sequence ID" value="ENSGAGP00000023020.1"/>
        <property type="gene ID" value="ENSGAGG00000016891.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000026239.1">
        <property type="protein sequence ID" value="ENSGAGP00000023022.1"/>
        <property type="gene ID" value="ENSGAGG00000016891.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000026240.1">
        <property type="protein sequence ID" value="ENSGAGP00000023023.1"/>
        <property type="gene ID" value="ENSGAGG00000016891.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000026242.1">
        <property type="protein sequence ID" value="ENSGAGP00000023025.1"/>
        <property type="gene ID" value="ENSGAGG00000016891.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061001">
        <property type="term" value="P:regulation of dendritic spine morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045329">
        <property type="entry name" value="LZTS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19354">
        <property type="entry name" value="ZIPPER PUTATIVE TUMOR SUPPRESSOR 2 HOMOLOG-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19354:SF6">
        <property type="entry name" value="ZIPPER PUTATIVE TUMOR SUPPRESSOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06818">
        <property type="entry name" value="Fez1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="118"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="170"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="632"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="306"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="490"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="556"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="125"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="190"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="632"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="643"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="662" mass="75672" checksum="883016C07FE7E739" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7L0</accession>
    <name>A0A452I7L0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I7L0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023616"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026908.1">
        <property type="protein sequence ID" value="ENSGAGP00000023616.1"/>
        <property type="gene ID" value="ENSGAGG00000017313.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000008">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000088">
        <property type="entry name" value="Zinc finger and SCAN domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000180">
        <property type="entry name" value="Zinc finger protein 689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF48">
        <property type="entry name" value="ZINC FINGER PROTEIN 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="470"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="498"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="526"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="553" mass="61103" checksum="EB7C4B347987FE4F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7L1</accession>
    <name>A0A452I7L1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger and BTB domain-containing protein 14</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene. Transcriptional repressor of MYC and thymidine kinase promoters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with ZBTB21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I7L1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023513"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026798.1">
        <property type="protein sequence ID" value="ENSGAGP00000023513.1"/>
        <property type="gene ID" value="ENSGAGG00000017239.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18204">
        <property type="entry name" value="BTB_POZ_ZBTB14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000147">
        <property type="entry name" value="POZ-, AT hook-, and zinc finger-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000316">
        <property type="entry name" value="Zinc finger and BTB domain-containing 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000762">
        <property type="entry name" value="Zinc finger and BTB domain-containing 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000766">
        <property type="entry name" value="Zinc finger and BTB domain-containing 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000054">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF28">
        <property type="entry name" value="ZINC FINGER PROTEIN 37-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="17">
        <location>
            <begin position="36"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="387"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="152"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="448" mass="50955" checksum="11DED2BCEF7B6396" modified="2019-05-08" version="1">MEFFISMSESIKYNDDDHKTVFLKTLNEQRLEGEFCDIAIVVEDVKFRAHRCVLAACSTYFKKLFKKLEVDSSSVIEIDFLRSDIFEEVLNYMYTAKISVKKEDVNLMMSSGQILGIRFLDKLCSQKRDVSSPEENAQSKSKYCLKINRSIGESNDNQDDEVEEIGDHDDSPSDVTVEGTPPSQEDGKSPTNTLRVQEAILKELGSEEVRKVNCYGQEVEPMETTESKDLGSQTPQALTFNDGISEVKDEQTPGWTTAAADMKFEYLLYGHREQIACQACGKTFTDEARLRKHEKLHTADRPFVCEMCTKGFTTQAHLKEHLKIHTGYKPYSCEVCGKSFIRAPDLKKHERVHSNERPFACHMCDKAFKHKSHLKDHERRHRGEKPFVCSSCTKAFAKASDLKRHENNMHSERKQITTANAIQSETEQLQAAAMAAEAEQQLETIACS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8V6</accession>
    <name>A0A452I8V6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">5'-3' exoribonuclease 1</fullName>
            <ecNumber evidence="9">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the 5'-3' exonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023976"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027305.1">
        <property type="protein sequence ID" value="ENSGAGP00000023976.1"/>
        <property type="gene ID" value="ENSGAGG00000017535.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1050:FF:000001">
        <property type="entry name" value="5'-3' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016494">
        <property type="entry name" value="5_3_exoribonuclease_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041385">
        <property type="entry name" value="SH3_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040992">
        <property type="entry name" value="XRN1_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047007">
        <property type="entry name" value="XRN1_D1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041106">
        <property type="entry name" value="XRN1_D2_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047008">
        <property type="entry name" value="XRN1_SH3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF7">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18129">
        <property type="entry name" value="SH3_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18332">
        <property type="entry name" value="XRN1_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18334">
        <property type="entry name" value="XRN1_D2_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006743">
        <property type="entry name" value="Exonuclease_Xnr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exoribonuclease 1 D1" evidence="14">
        <location>
            <begin position="652"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease Xrn1 D2/D3" evidence="15">
        <location>
            <begin position="850"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exoribonuclease 1 SH3-like" evidence="13">
        <location>
            <begin position="1102"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1298"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1493"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1327"/>
            <end position="1349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF18129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1695" mass="193484" checksum="15525D6B13D99FB2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I938</accession>
    <name>A0A452I938_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I938"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024190"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027540.1">
        <property type="protein sequence ID" value="ENSGAGP00000024190.1"/>
        <property type="gene ID" value="ENSGAGG00000017689.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106018">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14584">
        <property type="entry name" value="PTP-MTMR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000135">
        <property type="entry name" value="Myotubularin related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030591">
        <property type="entry name" value="MTMR8_PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048994">
        <property type="entry name" value="PH-GRAM_MTMR6-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF36">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21098">
        <property type="entry name" value="PH-GRAM_MTMR6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="7">
        <location>
            <begin position="134"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="607"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="607"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="631"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="4">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="283"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="346"/>
            <end position="352"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="641" mass="73721" checksum="85C11590095254B7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9R2</accession>
    <name>A0A452I9R2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I9R2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024421"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027797.1">
        <property type="protein sequence ID" value="ENSGAGP00000024421.1"/>
        <property type="gene ID" value="ENSGAGG00000017843.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037394">
        <property type="entry name" value="TBATA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33772:SF3">
        <property type="entry name" value="PROTEIN TBATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33772">
        <property type="entry name" value="THYMUS, BRAIN AND TESTES-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15256">
        <property type="entry name" value="SPATIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="375" mass="41478" checksum="3894889184CD9E16" modified="2019-05-08" version="1">MAGMGRITKMATEIKNLEPVLQQLETKCISAKEKMEMLRSPVAGPVKGKDITALAGKLKHVALNTPLRFSTEATRPQSKNNSRFGNLSHHSFFSRHNPHPHRVTHIQGLNGVPICIVNDGWSVLTPLSPHPMIKGQLSTTVLGVPGAQMPIGDPHSNLVPRLTVGSLSDAWREELRELAARVSASSSTEMEQKEVAEEPRRATQYSAETGRLIPPSSRATVRHTPQQLCRNNAKNKGKDAALSFQDQELVILELLCQILQTDSLSAIQQWLLTAGQREKDLVMRMLQTATANLQPEPKSLSTNLEQRLQSQMSQTAVGLSSRGQHLGRNHPVRLSQSQKQEPIPEEDKPVHMGTAEVLQFHLFRDGKQNQPDPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9X3</accession>
    <name>A0A452I9X3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I9X3"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027864.1">
        <property type="protein sequence ID" value="ENSGAGP00000024484.1"/>
        <property type="gene ID" value="ENSGAGG00000017889.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="134" mass="14753" checksum="5B957A579A49DD0F" modified="2019-05-08" version="1">MKARQPLVDTGSSISSHFTSTTVSSARERLLWLIDLMEVGSFQWGGELRAVVSIVKGMGKYAGWEHNPIACCRRLMRLGALTLNSCSPIWCWFSLPLPPPTLSFSLASPSLPLVLGRQCLELQCRQGTDVFTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB35</accession>
    <name>A0A452IB35_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IB35"/>
    <dbReference type="SMR" id="A0A452IB35"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024722"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028130.1">
        <property type="protein sequence ID" value="ENSGAGP00000024722.1"/>
        <property type="gene ID" value="ENSGAGG00000018045.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000084">
        <property type="entry name" value="Homeobox protein Hox-D13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051003">
        <property type="entry name" value="AP_axis_regulatory_Homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022067">
        <property type="entry name" value="HoxA13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45804:SF6">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45804">
        <property type="entry name" value="SEGMENTATION PROTEIN FUSHI TARAZU-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12284">
        <property type="entry name" value="HoxA13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="225"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="227"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="295" mass="32203" checksum="12DA269095C3D98F" modified="2019-05-08" version="1">MDPGTFARAEELDESTKTMEGLMGAGSFAASQCRNLVAHSALGGPPPPALMQGSTYSNLDAPNAGPGDPSKQCIPCPAIPQSSSTTPPLPYGYFGGAYYSCRVARSSLKPCPQSSYSAEKYMDTPAAADDYQTRPTELAFYPGYAGPYQPRASYLDVSVVQTIGGPGEPRHETLLPVDSYHQPWALTSGWNSQMCCPKDQSQAGHFWKSAFADVGQPPPDACSFRRGRKKRIPYSKGQLKELEKEYASSKFITKDKRRKIAAATNLTERQITIWFQNRRVKEKKVVAKVKTSSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBS3</accession>
    <name>A0A452IBS3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IBS3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025211"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028702.1">
        <property type="protein sequence ID" value="ENSGAGP00000025211.1"/>
        <property type="gene ID" value="ENSGAGG00000018442.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15911">
        <property type="entry name" value="7tmA_OR11A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="44"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="320" mass="35909" checksum="E43851F4CD3A77DC" modified="2019-05-08" version="1">MTNIHEGNQSTITKFILLGFGDLPQLHILLFLLFLVIYIVTMAGNILVIVLVVTDQHLHTPMYFFLGNLSCLETCCTSTILPRVLASLLTGDRSISVMGCITQFYFFGSLAGTECLLLSVMSYDRYLAICKPLHYATRMNGRLCLQLVVGLWIGGFMSVSIFIFMLSQLKFCAPNEIDHFFCDFHPITKLSCTDTHIVEAAGLISCAMFTLPPFVLTVASYVYIISNIMRIPSTAGRQKAFSTCSSHLIVVTIYYGTLILVYLLSDSDALRDLNQVFSVFYGVLTPLVNPLIYSLRNKEVKKALRKALLRSFVFFRNSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ID43</accession>
    <name>A0A452ID43_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha-2C adrenergic receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Alpha-2C adrenoreceptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ID43"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025628"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029161.1">
        <property type="protein sequence ID" value="ENSGAGP00000025628.1"/>
        <property type="gene ID" value="ENSGAGG00000018715.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004938">
        <property type="term" value="F:alpha2-adrenergic receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051379">
        <property type="term" value="F:epinephrine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030168">
        <property type="term" value="P:platelet activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006940">
        <property type="term" value="P:regulation of smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019229">
        <property type="term" value="P:regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15323">
        <property type="entry name" value="7tmA_alpha2C_AR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000100">
        <property type="entry name" value="alpha-2B adrenergic receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000245">
        <property type="entry name" value="Alpha-2C adrenergic receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002233">
        <property type="entry name" value="ADR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000735">
        <property type="entry name" value="ADRA2C_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF25">
        <property type="entry name" value="ALPHA-2C ADRENERGIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01103">
        <property type="entry name" value="ADRENERGICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00560">
        <property type="entry name" value="ADRENRGCA2CR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="57"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="286"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="309"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="436" mass="49758" checksum="26502AB776BAD319" modified="2019-05-08" version="1">MDLLVLMNISAGTPNGSLAFPSSSSSPLQQPPSHYSPGAVASLAAVVSFLIVFTIVGNVLVVIAVLTSKALRAPQNLFLVSLASADILVATLVMPFSLANEIMDYWYFGKAWCNIYLALDVLFCTSSIVHLCAISLDRYWSVTQAVEYNLKRTPRRIKGIILTVWLISAVISFPPLISVYRDPEGDLFPLCKLNDETWYILSSCIGSFFAPCLIMVLVYVRIYRVAKLRTRTLSEKRTVPEGSSLSENGLSRAHRGCMSLRMQPGENGHYSVHQWRKASELEDIELEESSTSESRRRRSRDDHPHKSSKSQSFSYSYSSKRSSNRSRDLFSSHRRRKRSSVCRKKVTQAREKRFTFVLAVVMGVFVVCWFPFFFSYSLYGICRKACEVPETLFKFFFWIGYCNSSLNPVIYTIFNQDFRRSFKHILFKKKKKNFLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDZ4</accession>
    <name>A0A452IDZ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GCF C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GCF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDZ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026017"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029592.1">
        <property type="protein sequence ID" value="ENSGAGP00000026017.1"/>
        <property type="gene ID" value="ENSGAGG00000019007.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012890">
        <property type="entry name" value="GCFC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022783">
        <property type="entry name" value="GCFC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12214">
        <property type="entry name" value="GC-RICH SEQUENCE DNA-BINDING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12214:SF4">
        <property type="entry name" value="INTRON LARGE COMPLEX COMPONENT GCFC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07842">
        <property type="entry name" value="GCFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GCF C-terminal" evidence="6">
        <location>
            <begin position="374"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="291"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="178"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="302"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="649" mass="74619" checksum="A8706A036C1FD4EF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IE04</accession>
    <name>A0A452IE04_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000026030"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029606.1">
        <property type="protein sequence ID" value="ENSGAGP00000026030.1"/>
        <property type="gene ID" value="ENSGAGG00000019014.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21545:SF14">
        <property type="entry name" value="LIGAND-DEPENDENT COREPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21545">
        <property type="entry name" value="TRANSCRIPTION FACTOR MLR1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="75" mass="8544" checksum="7E58AC6DC08CE111" modified="2019-05-08" version="1">MDSARRPWACRCGALPQKMASPCGRQECSIERRGFRHQLDSWRHKLXTARFESILEGLFGPGLLKDLSLFKGIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IEE2</accession>
    <name>A0A452IEE2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TMEM179 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IEE2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025846"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029402.1">
        <property type="protein sequence ID" value="ENSGAGP00000025846.1"/>
        <property type="gene ID" value="ENSGAGG00000018876.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029673">
        <property type="entry name" value="TMEM179"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059010">
        <property type="entry name" value="TMEM179-179B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31872">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31872:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26158">
        <property type="entry name" value="Claudin_TMEM179-179B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="100" mass="11564" checksum="4DA79E4C10D63654" modified="2019-05-08" version="1">MWCDAVTEKGSMPNSCEELQDIDLELNLETSAFYDQFAIAQFGLWAAWLTWLGITILAFLKVYHNYRQEDLLDSLIHEKELLLGRSSSRTFLQDEKSGMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHP0</accession>
    <name>A0A452IHP0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IHP0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027024"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030706.1">
        <property type="protein sequence ID" value="ENSGAGP00000027024.1"/>
        <property type="gene ID" value="ENSGAGG00000019660.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000030708.1">
        <property type="protein sequence ID" value="ENSGAGP00000027026.1"/>
        <property type="gene ID" value="ENSGAGG00000019660.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.10:FF:000001">
        <property type="entry name" value="Cystatin-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001713">
        <property type="entry name" value="Prot_inh_stefin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414">
        <property type="entry name" value="CYSTATIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414:SF20">
        <property type="entry name" value="CYSTATIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00295">
        <property type="entry name" value="STEFINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="7">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="100" mass="11065" checksum="245BFEE47A2822A1" modified="2019-05-08" version="1">MSAGGLTETKPATPEIQKIADQVKLQLEGKTNEKYLVFVAIQYKAQVVAGINYFIKVSVSNSTDECVHLRVFQSLPHENKGPSLTSYETGKTKADPLNYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452II37</accession>
    <name>A0A452II37_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Nucleolar protein 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
            <location evidence="2 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NRAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452II37"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027512"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031252.1">
        <property type="protein sequence ID" value="ENSGAGP00000027512.1"/>
        <property type="gene ID" value="ENSGAGG00000019996.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000005">
        <property type="entry name" value="Nucleolar protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000006">
        <property type="entry name" value="Nucleolar protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3030:FF:000001">
        <property type="entry name" value="Nucleolar protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005554">
        <property type="entry name" value="NOL6/Upt22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035082">
        <property type="entry name" value="Nrap_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035367">
        <property type="entry name" value="Nrap_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035368">
        <property type="entry name" value="Nrap_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035369">
        <property type="entry name" value="Nrap_D4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035370">
        <property type="entry name" value="Nrap_D5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035371">
        <property type="entry name" value="Nrap_D6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972">
        <property type="entry name" value="NUCLEOLAR RNA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03813">
        <property type="entry name" value="Nrap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17403">
        <property type="entry name" value="Nrap_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17404">
        <property type="entry name" value="Nrap_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17405">
        <property type="entry name" value="Nrap_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17406">
        <property type="entry name" value="Nrap_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17407">
        <property type="entry name" value="Nrap_D6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nrap protein" evidence="11">
        <location>
            <begin position="157"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="12">
        <location>
            <begin position="303"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="13">
        <location>
            <begin position="447"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="14">
        <location>
            <begin position="621"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="15">
        <location>
            <begin position="827"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="16">
        <location>
            <begin position="984"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF17407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1128" mass="126988" checksum="89DC45E4740702C1" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIC9</accession>
    <name>A0A452IIC9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">mRNA decay activator protein ZFP36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Zinc finger protein 36</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Functions by recruiting the CCR4-NOT deadenylase complex and probably other components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes. Binds to 3'-UTR ARE of numerous mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Associates with the cytoplasmic CCR4-NOT deadenylase complex to trigger ARE-containing mRNA deadenylation and decay processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000027589"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031343.1">
        <property type="protein sequence ID" value="ENSGAGP00000027589.1"/>
        <property type="gene ID" value="ENSGAGG00000020047.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061158">
        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900153">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000002">
        <property type="entry name" value="Zinc finger protein 36, C3H1 type-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007635">
        <property type="entry name" value="Tis11B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF174">
        <property type="entry name" value="MRNA DECAY ACTIVATOR PROTEIN ZFP36L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04553">
        <property type="entry name" value="Tis11B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="188"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="188"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="62"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="216"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="338"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="412"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="257"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="439"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="466"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="491" mass="53371" checksum="B7A5AC488861088F" modified="2019-05-08" version="1">MSTTLLSAFYDIDFLCKSEKALTNLSSMLDKKAVGTPVTSPSSSFAPGFLRRHSTSNLQALANSSKFPSSSGSSSSSSSSSSSFGSLKETGTGGGGGGGSSSPTALLNKENKFRDRSFSENGERSQHLMQQLQQQQAGKGGGGAPINSTRYKTELCRPFEESGACKYGEKCQFAHGFHELRSLTRHPKYKTELCRTFHTIGFCPYGPRCHFIHNADERRPAPGGGTAAPAAPHHHPHPPPPPPHPAGSTXXXXXXXXRRRVRAPARRWRAAQAAPQPELLRLLRPPPARLRAAARRAPGGGAAREPRXXXXPAAARLRLLLRRAALAALRQQRLRLLGPGAGQPHRAARHPQPQLRRQPRRRRRRGSCRRLLPLPAAATAARGELPAAARLAPLQLPAPAPPLRVPRLRRAAQPPGLALRPRELPERLPQLRQPERLRVPQPGLRPAPAHLQPPLHLRRLGGAGPERGERRGGGEPRGRGQDKPREQRPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIY9</accession>
    <name>A0A452IIY9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MDFI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IIY9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027762"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031538.1">
        <property type="protein sequence ID" value="ENSGAGP00000027762.1"/>
        <property type="gene ID" value="ENSGAGG00000020181.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050434">
        <property type="term" value="P:positive regulation of viral transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026134">
        <property type="entry name" value="MDFI/MDFIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15304">
        <property type="entry name" value="MYOD FAMILY INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15304:SF0">
        <property type="entry name" value="MYOD FAMILY INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15316">
        <property type="entry name" value="MDFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="249" mass="26642" checksum="27671C8D4696AB0E" modified="2019-05-08" version="1">MSQAREPLPPGPDGPAPAAPTERSLLVSLPREKCDKVNTAAGERDITPPSNNQSIQPEIQDQSVWGNRTDGDLIRTQPQHFPQPNTSALEKSEEETGKIQNGHAGLSNGNGIHNGVKHVATDNRKCSVPVSQKMHRKIQSSLSVNSDSSKKSKISSAYSQKPGSSPEDCCVHCILACLFCEFLTLCNIVLGQASCGICTSETCCCCCGDEMGDDCNCPCDMDCGIMDTCCESSDCLEICMECCGICFPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJ19</accession>
    <name>A0A452IJ19_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PIPK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJ19"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027862"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031650.1">
        <property type="protein sequence ID" value="ENSGAGP00000027862.1"/>
        <property type="gene ID" value="ENSGAGG00000020247.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17308">
        <property type="entry name" value="PIPKc_PIP5K1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000001">
        <property type="entry name" value="phosphatidylinositol 4-phosphate 5-kinase type-1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF26">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-PHOSPHATE 5-KINASE TYPE-1 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="5">
        <location>
            <begin position="77"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="527"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="728" mass="80348" checksum="13659F8CF459A8C7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJA8</accession>
    <name>A0A452IJA8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Adipocyte plasma membrane-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJA8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027953"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031752.1">
        <property type="protein sequence ID" value="ENSGAGP00000027953.1"/>
        <property type="gene ID" value="ENSGAGG00000020304.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004064">
        <property type="term" value="F:arylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000041">
        <property type="entry name" value="adipocyte plasma membrane-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF130">
        <property type="entry name" value="ADIPOCYTE PLASMA MEMBRANE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20067">
        <property type="entry name" value="SSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="10">
        <location>
            <begin position="200"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="415" mass="46500" checksum="311282A6A055B760" modified="2019-05-08" version="1">MNEAEGLRQRRAVRPQVITEDTPAQEAKDGSTYSSKVFRVTFMILAVSLIIPLLGAIVLLECPIDPQPLSFKEPPLLTGVLKPNIKLRQAERIFENQLIGPESIANIGDVLFTGTADGKILKIEDGEIKTLARLGHGPCRTREDEPTCGRPLGIRVGPTNTLFVADAYYGIYEIDTVTGDVKMLLSTSEPVEDHKLSFVNDLTLTRDGRKIFFTDSSSKWQRRDYSFLVMEGTDDGRLLEYDTVTKEVKVLMVGLRFPNGVQLSPAEDFVLVAETTLARIRRYYVSGLMKGGADMFVENMPGFPDNIRLSSSGGYWVAMSAVRPNPGFSLVDFISEKPWIKRIIFKLLSQETVIKFVPKYSLVVELSETGSYRRSFHDPNGMVVTYVSEAHEHNGYLYLGSFRSPFICRLNLEHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IP69</accession>
    <name>A0A452IP69_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IP69"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029681"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033702.1">
        <property type="protein sequence ID" value="ENSGAGP00000029681.1"/>
        <property type="gene ID" value="ENSGAGG00000021425.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010387">
        <property type="term" value="P:COP9 signalosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041481">
        <property type="entry name" value="CSN7_helixI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF7">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22061">
        <property type="entry name" value="CSN7_HB_subdom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18392">
        <property type="entry name" value="CSN7a_helixI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="10">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="227"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="263" mass="29102" checksum="2122BA60FDF48905" modified="2019-05-08" version="1">MAAEVKVTGQSQEQLLVLARSARGAALVSLIHQVLEAPGIYVFGELLDLPNVQELAENEFSSVFRLLTLFAYGTYADYLSEAGNLPPLSEAQKNKLRHLSVVTLAAKIKCIPYAVLLEQLQLKNVRQLEDLVIEAVYADVLRGSLDQRNQRLEVDYSIGRDIRREELSTITRTLQEWCLGCEVVLSGIEEQVSRANQHKEQQLGLKQQIESEVANLKKTIKVTTAAAAAATSQDPEQHLTELREPAPGTNQRQTSKKASKAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQ96</accession>
    <name>A0A452IQ96_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Collectin-12</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IQ96"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030018"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034088.1">
        <property type="protein sequence ID" value="ENSGAGP00000030018.1"/>
        <property type="gene ID" value="ENSGAGG00000021667.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000017">
        <property type="entry name" value="collectin-12 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058762">
        <property type="entry name" value="COLEC12_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26004">
        <property type="entry name" value="COLEC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0176">Collagen</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="C-type lectin" evidence="17">
        <location>
            <begin position="558"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="389"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="473"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="521"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="681" mass="74823" checksum="20DCFA59648D6211" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQN2</accession>
    <name>A0A452IQN2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IQN2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000487"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000546.1">
        <property type="protein sequence ID" value="ENSGAGP00000000487.1"/>
        <property type="gene ID" value="ENSGAGG00000000408.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000001689.1">
        <property type="protein sequence ID" value="ENSGAGP00000001481.1"/>
        <property type="gene ID" value="ENSGAGG00000001191.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000024096.1">
        <property type="protein sequence ID" value="ENSGAGP00000021157.1"/>
        <property type="gene ID" value="ENSGAGG00000015545.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000026675.1">
        <property type="protein sequence ID" value="ENSGAGP00000023421.1"/>
        <property type="gene ID" value="ENSGAGG00000017161.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000034514.1">
        <property type="protein sequence ID" value="ENSGAGP00000030415.1"/>
        <property type="gene ID" value="ENSGAGG00000021902.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000036505.1">
        <property type="protein sequence ID" value="ENSGAGP00000032201.1"/>
        <property type="gene ID" value="ENSGAGG00000023050.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01345">
        <property type="entry name" value="CERVTRCPTASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="333" mass="38668" checksum="82239C77B5053651" modified="2019-05-08" version="1">MGMNMKVVRWIRNWLKGRLQRVVLKGELSGWKEVTSGVPQGSVLGPILFNLFITDLGTKSGNVLIKFADDTKLGGIANTEKDRDTIQEDLNHLVNWSNRNRMKYNSEKCKVMHLGINNKNFGYTLGAHQLEATEEEKDLGVLVDSRMTMSRQCDTAVKKANAILGCIRRGISCKDKEVLVPLYKALVRPHLEYCVQFWCPMFKKDEFKLEQVQRRATRMIRGMEKLPYERRLKELGLFSLAKRRLRGDMLALYKYIRGVNVREGEELFKFSTNVGTRTNGYKLDIRKFRLEIRRRFLTIRGVKFWNSLPREVVGAKDFPGFKTKLDKYMEGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITX3</accession>
    <name>A0A452ITX3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ITX3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031555"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035786.1">
        <property type="protein sequence ID" value="ENSGAGP00000031555.1"/>
        <property type="gene ID" value="ENSGAGG00000022661.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="61" mass="6728" checksum="66DB62B8C9A3BE55" modified="2019-05-08" version="1">MSVAEHRFEPRLLLQEVTMAGLHELRFTEEKPLLRGQDTELVSCLAAPQDPVSVAGPAGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUC9</accession>
    <name>A0A452IUC9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubiquitin family. SUMO subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IUC9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031500"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035724.1">
        <property type="protein sequence ID" value="ENSGAGP00000031500.1"/>
        <property type="gene ID" value="ENSGAGG00000022624.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16115">
        <property type="entry name" value="Ubl_SUMO2_3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000482">
        <property type="entry name" value="Small ubiquitin-related modifier 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="5">
        <location>
            <begin position="125"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="204" mass="22421" checksum="474E8FCF52ABD99B" modified="2019-05-08" version="1">EDNARRTGTAQPIAPGGVGDAHARLGDSRARARSFSRPCYVTDQRAPGGRTQWGLGWGAARARARRVKRGPAAVRARSPQPPPLSLFASLGLSPPFVKRSEQPGPCCLAMADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLNKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IV45</accession>
    <name>A0A452IV45_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Histone-lysine N-methyltransferase ASH1L</fullName>
            <ecNumber evidence="4">2.1.1.359</ecNumber>
            <ecNumber evidence="29">2.1.1.367</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">ASH1-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Absent small and homeotic disks protein 1 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="40 41">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="41" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="40" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="40" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="28">Histone methyltransferase specifically trimethylating 'Lys-36' of histone H3 forming H3K36me3. Also monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro. The physiological significance of the H3K9me1 activity is unclear.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60324"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:15536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>L-lysyl(9)-[histone H3] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl(9)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60280"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="Rhea" id="RHEA-COMP:15546"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="EC" id="2.1.1.367"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.359" evidence="4"/>
    <dbReference type="EC" id="2.1.1.367" evidence="29"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031746"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036002.1">
        <property type="protein sequence ID" value="ENSGAGP00000031746.1"/>
        <property type="gene ID" value="ENSGAGG00000022804.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140955">
        <property type="term" value="F:histone H3K36 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140948">
        <property type="term" value="F:histone H3K9 monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04717">
        <property type="entry name" value="BAH_polybromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05525">
        <property type="entry name" value="Bromo_ASH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15548">
        <property type="entry name" value="PHD_ASH1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19174">
        <property type="entry name" value="SET_ASH1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000025">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000011">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000008">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000113">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006560">
        <property type="entry name" value="AWS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043320">
        <property type="entry name" value="Bromo_ASH1L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043319">
        <property type="entry name" value="PHD_ASH1L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147:SF1">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASH1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17907">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00570">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51215">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="20" id="KW-0007">Acetylation</keyword>
    <keyword evidence="23" id="KW-0010">Activator</keyword>
    <keyword evidence="22 33" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="19" id="KW-0965">Cell junction</keyword>
    <keyword evidence="18" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="25" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="41" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0796">Tight junction</keyword>
    <keyword evidence="24" id="KW-0804">Transcription</keyword>
    <keyword evidence="21" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <keyword evidence="15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AWS" evidence="39">
        <location>
            <begin position="2128"/>
            <end position="2179"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="36">
        <location>
            <begin position="2182"/>
            <end position="2298"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="37">
        <location>
            <begin position="2306"/>
            <end position="2322"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="35">
        <location>
            <begin position="2499"/>
            <end position="2569"/>
        </location>
    </feature>
    <feature type="domain" description="BAH" evidence="38">
        <location>
            <begin position="2697"/>
            <end position="2834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="101"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="159"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="223"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="581"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="736"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="868"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1141"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1188"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1292"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1529"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1659"/>
            <end position="1692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1705"/>
            <end position="1743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1787"/>
            <end position="1813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1967"/>
            <end position="2036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="2325"/>
            <end position="2382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="2859"/>
            <end position="2893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="2909"/>
            <end position="2945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="34">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="34">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="603"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="34">
        <location>
            <begin position="1148"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="34">
        <location>
            <begin position="1201"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="1225"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="34">
        <location>
            <begin position="1305"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="1535"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="1983"/>
            <end position="1993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="34">
        <location>
            <begin position="1999"/>
            <end position="2010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="2326"/>
            <end position="2340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="34">
        <location>
            <begin position="2341"/>
            <end position="2363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="2371"/>
            <end position="2382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="34">
        <location>
            <begin position="2878"/>
            <end position="2891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="34">
        <location>
            <begin position="2915"/>
            <end position="2938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00051161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00057835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00066816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00070988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00077263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00078568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="38">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="39">
        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="3005" mass="335975" checksum="952B7AABAE070564" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IVU1</accession>
    <name>A0A452IVU1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G-protein coupled receptor 22</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IVU1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032200"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036504.1">
        <property type="protein sequence ID" value="ENSGAGP00000032200.1"/>
        <property type="gene ID" value="ENSGAGG00000023049.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000084">
        <property type="entry name" value="Probable G-protein coupled receptor 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000116">
        <property type="entry name" value="probable G-protein coupled receptor 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="344"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="58"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="433" mass="49110" checksum="2B3768A0A2371FFB" modified="2019-05-08" version="1">MCFSPILEVNMQSESNITVRDAIDDINTNMYRALSYPLSFQVSLTGFLMLEIVLGLGSNLTVLVLYCMKSNLINSVSNIITMNLHVLDVIICVGCIPLTIVILLLSLESNSALICCFHEACVSFASVATAINVFAITLDRYDISVKPANRILTLGRAVMLMTSIWIVSLFSFLIPFIEVNFFSLQSASTWENKTLLCVSVNEYHTELGMYYHLLVQIPIFFFTVIVMLITYTKILQALNIRIGTRFTTGQKKKARKKKTISLTTQHETTDVSQSSGGRNVVFGVRTSVSVIIALRRAVKRHRERRERQKRVFRMSLLIISTFLLCWTPISVLNTTILCLGPSDLLVKLRLCFLVMAYGTTIFHPLLYAFTRQKFQKVLKSKMKKRVVSIVEADPMPNNAVIHNSWIEPKRKKQITFEDNEVRQKCLVPQVVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWC1</accession>
    <name>A0A452IWC1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IWC1"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036502.1">
        <property type="protein sequence ID" value="ENSGAGP00000032198.1"/>
        <property type="gene ID" value="ENSGAGG00000023047.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="138" mass="14578" checksum="0377FB75A8785650" modified="2019-05-08" version="1">MGHSLPIPYFIALDTVIYYLIALASLSDLQAKAVRPLPGREPRADPTRAPPRANTMFCLHPAAPGPARMRLGCRGHGRTPETPHGPLQGRAGLRCAARRSPLARAARGAPGRSAPGSPSEPQRRSCPSAHPAPHAPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWH0</accession>
    <name>A0A452IWH0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Amyloid beta precursor protein binding family B member 1 interacting protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IWH0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032398"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036729.1">
        <property type="protein sequence ID" value="ENSGAGP00000032398.1"/>
        <property type="gene ID" value="ENSGAGG00000023177.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039664">
        <property type="entry name" value="GRB/APBB1IP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243:SF14">
        <property type="entry name" value="AMYLOID BETA A4 PRECURSOR PROTEIN-BINDING FAMILY B MEMBER 1-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="19874" checksum="9E903915FECDC7EF" modified="2019-05-08" version="1">MEQTCDDIDEMFSNLLGEMDLLTQSLGVETVQPPSPKESNKEYNFTVGFKDLNESLSALEDNDLDALMADLVADLTEVEQKTVQAQKTSHYSQSEVTQPSTSVYAAASYDYKSNVPVTTTGRFEDDLPPPPPDPAFNLPLPPPPPEPLTQVSTEGTLPVVIGIKYNMVLQKVDRAKPTELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IX26</accession>
    <name>A0A452IX26_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IX26"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032617"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036969.1">
        <property type="protein sequence ID" value="ENSGAGP00000032617.1"/>
        <property type="gene ID" value="ENSGAGG00000023317.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07649">
        <property type="entry name" value="F-BAR_GAS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000024">
        <property type="entry name" value="growth arrest-specific protein 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037957">
        <property type="entry name" value="GAS7_F-BAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF57">
        <property type="entry name" value="GROWTH ARREST-SPECIFIC PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16623">
        <property type="entry name" value="WW_FCH_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="F-BAR" evidence="5">
        <location>
            <begin position="109"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="18"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="221"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="389" mass="45191" checksum="5FD53CDAA74E2F37" modified="2019-05-08" version="1">MSPQGHRYYVNTFTNETTWERPSSVPGTPRSLGSQKSAVPAANGYHTPVTPMHQMEPGHMSLRKPSGDPQSPGSPRVPRRLARESSRTINCVTFPHPDTMQEQQLLKPTEWSYCDYFWADKKDPQGNNTVSGFEILLQKQLKGKQMQKEMAEFVRERIKIEEEYAKNLSKLSQNSLAAQEEGTLGEAWAQLKKSLADEAEVHLKFSSKLQSEVEKPLLNFRENFKKDMKKYDHHIADLRKQLASRYTAVEKARKALTERQKDLEMKTQQLEIKLSNKAEEEIKKARRKSTQAGDDLMRCVDLYNQAQSKWFEEMVTSTLELERLEVERVEMIRQHLCQYTQLRHETDMFNQSTIDPVDQLLQKVEPAKDRELWVQEHKTGDIRPVDMEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZM9</accession>
    <name>A0A452IZM9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Elbow/Noc family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZM9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033579"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038042.1">
        <property type="protein sequence ID" value="ENSGAGP00000033579.1"/>
        <property type="gene ID" value="ENSGAGG00000023914.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000129">
        <property type="entry name" value="Zinc finger protein 503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051520">
        <property type="entry name" value="Elbow/Noc_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022129">
        <property type="entry name" value="Tscrpt_rep_NocA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12522:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN 703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12522">
        <property type="entry name" value="ZINC-FINGER PROTEIN NOLZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12402">
        <property type="entry name" value="nlz1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="434"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="98"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="193"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="235"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="563" mass="56738" checksum="468E26EF5CD9A178" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZY7</accession>
    <name>A0A452IZY7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">POU-specific domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZY7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033661"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038127.1">
        <property type="protein sequence ID" value="ENSGAGP00000033661.1"/>
        <property type="gene ID" value="ENSGAGG00000023960.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF125">
        <property type="entry name" value="POU DOMAIN, CLASS 3, TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="8">
        <location>
            <begin position="33"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="120" mass="13168" checksum="36AAD4121877C386" modified="2019-05-08" version="1">ELTLLIFLRGKTRNPPRAPLSPLRNIFCFFCQDTTPTSEELEQFAKDLKHKRITLGFTQADVGMALGTLYGECFTFLPHPRRPSGASGGVSWCRSLQEVSGDGAGQIRSDQGLAQPNGQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J019</accession>
    <name>A0A452J019_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Otopetrin 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J019"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038162.1">
        <property type="protein sequence ID" value="ENSGAGP00000033693.1"/>
        <property type="gene ID" value="ENSGAGG00000023980.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015252">
        <property type="term" value="F:proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004878">
        <property type="entry name" value="Otopetrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522:SF63">
        <property type="entry name" value="OTOPETRIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522">
        <property type="entry name" value="PROTON CHANNEL OTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03189">
        <property type="entry name" value="Otopetrin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="305"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="342"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="386"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="535"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="569"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="606" mass="66278" checksum="628E2E81C6544E8D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1R6</accession>
    <name>A0A452J1R6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Eyes absent homolog</fullName>
            <ecNumber evidence="15">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14 15">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the HAD-like hydrolase superfamily. EYA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A452J1R6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034320"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038861.1">
        <property type="protein sequence ID" value="ENSGAGP00000034320.1"/>
        <property type="gene ID" value="ENSGAGG00000024390.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000038873.1">
        <property type="protein sequence ID" value="ENSGAGP00000034331.1"/>
        <property type="gene ID" value="ENSGAGG00000024390.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000038879.1">
        <property type="protein sequence ID" value="ENSGAGP00000034337.1"/>
        <property type="gene ID" value="ENSGAGG00000024390.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001240">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02601">
        <property type="entry name" value="HAD_Eya"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12350:FF:000001">
        <property type="entry name" value="Eyes absent homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028472">
        <property type="entry name" value="EYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006545">
        <property type="entry name" value="EYA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042577">
        <property type="entry name" value="EYA_dom_metazoan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038102">
        <property type="entry name" value="EYA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01658">
        <property type="entry name" value="EYA-cons_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10190">
        <property type="entry name" value="EYES ABSENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10190:SF7">
        <property type="entry name" value="EYES ABSENT HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 15" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 15" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="208"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="230"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="13">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR628472-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR628472-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="534" mass="59065" checksum="CA55B4AF7B49F143" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J376</accession>
    <name>A0A452J376_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fatty acid desaturase CarF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J376"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034869"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039481.1">
        <property type="protein sequence ID" value="ENSGAGP00000034869.1"/>
        <property type="gene ID" value="ENSGAGG00000024783.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00199"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050207">
        <property type="term" value="F:plasmanylethanolamine desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008611">
        <property type="term" value="P:ether lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019547">
        <property type="entry name" value="Lipid_desat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052601">
        <property type="entry name" value="Plasmalogen_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48177:SF1">
        <property type="entry name" value="PLASMANYLETHANOLAMINE DESATURASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48177">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10520">
        <property type="entry name" value="Lipid_desat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid desaturase" evidence="7">
        <location>
            <begin position="99"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="284" mass="32377" checksum="C8F8FAD742505055" modified="2019-05-08" version="1">MASGGAECARLQANGAGTELEAEAVYAQEESECSSRRWGAQHVGARELAELYSPGKRLQEWISVILCFCLISFNFYSLLSYLSLDHTLSVIVGIFAGVFTADFLSGLVHWGADTWGSVELPIVGKAFIRPFREHHIDPTAITRHDFIETNGDNCMLTLVPLANMAYKFVSLSPEALYQTCPWECYVFALAIFVTLTNQIHKWSHTYFGLPRWVIFLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLEKIGFWRCLENIIQGVTGQKPRADDMKWAQKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J3V7</accession>
    <name>A0A452J3V7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">SH3 domain containing GRB2 like 3, endophilin A3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the endophilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J3V7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035127"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039774.1">
        <property type="protein sequence ID" value="ENSGAGP00000035127.1"/>
        <property type="gene ID" value="ENSGAGG00000024972.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016191">
        <property type="term" value="P:synaptic vesicle uncoating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07615">
        <property type="entry name" value="BAR_Endophilin_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11803">
        <property type="entry name" value="SH3_Endophilin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000053">
        <property type="entry name" value="endophilin-A1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000021">
        <property type="entry name" value="Endophilin-A2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032469">
        <property type="entry name" value="Endophilin-A3_BAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035824">
        <property type="entry name" value="Endophilin_A_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF45">
        <property type="entry name" value="ENDOPHILIN-A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01887">
        <property type="entry name" value="SPECTRNALPHA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="14">
        <location>
            <begin position="18"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="291"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="39967" checksum="9DAEA97C2FBB727B" modified="2019-05-08" version="1">MSVAGLKKQFHKASQLFSEKISGAEGTKLDEDFQEMERKIDVTSKAVAELLSKSTEYLQPNPAYRAKLGMLNTMSKIRGQVKTTGYPQTEGLLGDCMLRYGRELGEDSTFGNALLDVGESMKQMAEVKDSFDINVKQNFIDPLQLLQDKDLKEIGHHLKKLEGRRLDYDYKKKRLGKIPDEEVKQAVEKFEESKELAERSMFNFLENDVEQVSQLAVFIEAALDYHKQSTEILEELQSTLQNRINGASSRPKHEFKPKPVITSALEISDNQQHNGISYISSIKSSGSSVHMDQPCCQALYDFEPENEGELGFKEGDIITLTNQIDENWYEGMLNGESGFFPINYVEVMVPLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J705</accession>
    <name>A0A452J705_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J705"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036268"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041057.1">
        <property type="protein sequence ID" value="ENSGAGP00000036268.1"/>
        <property type="gene ID" value="ENSGAGG00000025700.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000147">
        <property type="entry name" value="GS homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042191">
        <property type="entry name" value="GSH1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47421:SF2">
        <property type="entry name" value="GS HOMEOBOX 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47421">
        <property type="entry name" value="GS HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="137"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="139"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="222"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="247" mass="27111" checksum="B8D1DDEDE93E2080" modified="2019-05-08" version="1">MPRSFLVDSLVLREASEKKSESSPPPPLFPYAVHPSHPLHGLPPGACHSRKAGLLCVCPLCVTASQLHPPPPALPLLKASFPPFGSQYCHAPLARQHSASSAVSVSHAPAIYQTAYPLPDPRQFHCISVDSTSSQLPSSKRMRTAFTSTQLLELEREFASNMYLSRLRRIEIATYLNLSEKQVKIWFQNRRVKHKKEGKSSSHRGGGSAHNCKCSSLATTKCSEDDEDLPMSPSSSGKDDRDLMVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GF97</accession>
    <name>A0A452GF97_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Centrosomal protein of 85 kDa-like CC4 coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GF97"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000235"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000243.1">
        <property type="protein sequence ID" value="ENSGAGP00000000213.1"/>
        <property type="gene ID" value="ENSGAGG00000000190.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000000267.1">
        <property type="protein sequence ID" value="ENSGAGP00000000235.1"/>
        <property type="gene ID" value="ENSGAGG00000000190.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058190">
        <property type="entry name" value="CC4_CEP85"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040210">
        <property type="entry name" value="Cep85/Cep85L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31075">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 85 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31075:SF3">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 85 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24555">
        <property type="entry name" value="CC4_CEP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centrosomal protein of 85 kDa-like CC4 coiled-coil" evidence="3">
        <location>
            <begin position="583"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="103"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="616"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="345"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="616"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="732" mass="82428" checksum="F9FADFCB297B2319" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFM0</accession>
    <name>A0A452GFM0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GFM0"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000408.1">
        <property type="protein sequence ID" value="ENSGAGP00000000363.1"/>
        <property type="gene ID" value="ENSGAGG00000000296.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="177" mass="18326" checksum="043BD5229EA8AB6E" modified="2025-10-08" version="2">MRRSPLGHPRQLLLGASLEVIPALLHLLARPLPAGDGIDLGSDALPDAPALGHRHLPALGAIQLGVVGAHLLDEDLVLLVGEAGLAQTGDLLSVPGHAWRPVERGRWGVPGGRGYPARRGGPHGGARGRGHLIPRAAPPLTARGSGSQQTQVASLCQLYPGQLPLSQQGAQALSRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJU0</accession>
    <name>A0A452GJU0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000001962"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002233.1">
        <property type="protein sequence ID" value="ENSGAGP00000001962.1"/>
        <property type="gene ID" value="ENSGAGG00000001581.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01221">
        <property type="entry name" value="PH_ephexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11940">
        <property type="entry name" value="SH3_ARHGEF5_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000007">
        <property type="entry name" value="rho guanine nucleotide exchange factor 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047271">
        <property type="entry name" value="Ephexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047270">
        <property type="entry name" value="PH_ephexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12845:SF2">
        <property type="entry name" value="DH DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12845">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="DH" evidence="7">
        <location>
            <begin position="1734"/>
            <end position="1918"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="1950"/>
            <end position="2060"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="2068"/>
            <end position="2129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="24"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="272"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="436"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="579"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="933"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1196"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1283"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1392"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="137"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="325"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="341"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="366"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="436"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="472"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="591"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="618"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="644"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="680"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="722"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="762"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="958"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1000"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1023"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1063"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1075"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1295"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1430"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1447"/>
            <end position="1456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2154" mass="234296" checksum="4F20414B81317AD3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJW6</accession>
    <name>A0A452GJW6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 21">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452GJW6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001894"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002153.1">
        <property type="protein sequence ID" value="ENSGAGP00000001894.1"/>
        <property type="gene ID" value="ENSGAGG00000001264.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12888">
        <property type="entry name" value="SPRY_PRY_TRIM7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020457">
        <property type="entry name" value="Znf_B-box_chordata"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001510">
        <property type="entry name" value="Znf_PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01406">
        <property type="entry name" value="BBOXZNFINGER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50064">
        <property type="entry name" value="ZF_PARP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="86"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="PARP-type" evidence="15">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="17">
        <location>
            <begin position="120"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="18">
        <location>
            <begin position="334"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="520" mass="59756" checksum="08D01CA96734B370" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJZ7</accession>
    <name>A0A452GJZ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GJZ7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002029"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002308.1">
        <property type="protein sequence ID" value="ENSGAGP00000002029.1"/>
        <property type="gene ID" value="ENSGAGG00000001634.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000332">
        <property type="entry name" value="Cathepsin L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="174" mass="19111" checksum="4711510E4BB8F8F2" modified="2019-05-08" version="1">MTPLSQQVLIDCSWGFGNYGCDGGEEWSAYEWIKKHGGIASTDSYGPYKGQNGLCHYNKSELVGKMKGYVNVTSGNITALKAAIYKNGPVAISIDASQRSFSFYSNGVYYEPKCGECNLDHAVLAVGYGVLQGELYWLVKNSWSTYWGNNGYILMSMKDNNCGVATDATYPILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GK00</accession>
    <name>A0A452GK00_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coiled-coil domain containing 70</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GK00"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002010"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002288.1">
        <property type="protein sequence ID" value="ENSGAGP00000002010.1"/>
        <property type="gene ID" value="ENSGAGG00000001621.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="25850" checksum="3CCEB0A14E4B76D3" modified="2019-05-08" version="1">KRGRDYEQMEQMSYTSRQKQLIKKLWDEKVFRKEIKAFQEKIKGFWEKMNAFREKIRAFRMAIQAFWEEENPIWEEENAFWEAEKTFREEAKAFWEGYRDFWKGYNAFWKKDKAFWKEDQFFWKKDKVLLDEDKVLWAEEKALWADEKALLEEERAFWEDEEALREDEKALQKDEKSIWEGAFGPLRGEQLLLAVVINAPGDHGPDILRGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GL45</accession>
    <name>A0A452GL45_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLUAP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GL45"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002388"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002738.1">
        <property type="protein sequence ID" value="ENSGAGP00000002388.1"/>
        <property type="gene ID" value="ENSGAGG00000001936.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019366">
        <property type="entry name" value="Clusterin-associated_protein-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21547">
        <property type="entry name" value="CLUSTERIN ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21547:SF0">
        <property type="entry name" value="CLUSTERIN-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10234">
        <property type="entry name" value="Cluap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="166"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="269" mass="30671" checksum="82D1F4D23D04E98B" modified="2019-05-08" version="1">FLTCCSLDFTCGRSNLGYKNHIRLVHASAHLATKAHIKLNTKKLYQADGYAVKELLKITSVLYNAMKTKGMETTSVSEEESSKFKFDLGSKIADLKAARQLASEITSRGASLYDLLGKEVELRELRTESIARPLEINETEKVMKIAIKDVLEQVQKTKDMLNNVASDEANLEAKIEKRKLELERNQKRLQTLQSETENTLRLMQNRLKEEEKRLLKSGSKLYFLTAWVRKMNYKLPAASFRKIPSKLRGNFISCLLGRLKSRKSKTPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLJ1</accession>
    <name>A0A452GLJ1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May act as a GTPase-activating protein for Rab family protein(s).</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GLJ1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002668"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003058.1">
        <property type="protein sequence ID" value="ENSGAGP00000002668.1"/>
        <property type="gene ID" value="ENSGAGG00000002129.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000027">
        <property type="entry name" value="Growth hormone regulated TBC protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000014">
        <property type="entry name" value="Growth hormone-regulated TBC protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000029">
        <property type="entry name" value="Growth hormone-regulated TBC protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF10">
        <property type="entry name" value="GROWTH HORMONE-REGULATED TBC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="42"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="298" mass="34566" checksum="E8D3C5D13B58AC89" modified="2019-05-08" version="1">YEEFFSRYLVILTRRAIKWSKLLKGNNRIQKSLKVKRYIRKGIPNEHRALIWMIVSGAQANMEQNPGYYNKLLEGEKSDTLVEAIKTDMNRTFPDNVQFRKTANPCLQQTLYNVLVAYGHHNKTVGYCQGMNFIAGYLILITKNEEESFWLLDALIGRILPDYYSPAMMGLKTDQEVLGELVRKKVPAVAELMDRHGVMWTLIVSRWFICLFIDILPVETVLRIWDCIFYEGSKIIFRVALALIKQHQAFILEATNLPDICDKFKQITRGTFVTECHTFMQVSVSSLQCRTGKSYETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPB0</accession>
    <name>A0A452GPB0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GPB0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003603"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004153.1">
        <property type="protein sequence ID" value="ENSGAGP00000003603.1"/>
        <property type="gene ID" value="ENSGAGG00000002905.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF142">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="14"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="104" mass="11558" checksum="E86B5DC610A46142" modified="2019-05-08" version="1">MSLHAGLLLFQGPVTFEEVAVYFTKEEGALLDPTQRALYRDVMQENYETVTSLGKDSFSKPDVVSQLEQGEEPWVLDLQGSEERGILRGVSDEKGGSFPSLRCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPK8</accession>
    <name>A0A452GPK8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prefoldin subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPK8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003839"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004449.1">
        <property type="protein sequence ID" value="ENSGAGP00000003839.1"/>
        <property type="gene ID" value="ENSGAGG00000003126.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23164">
        <property type="entry name" value="Prefoldin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000006">
        <property type="entry name" value="prefoldin subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="122" mass="14250" checksum="D99BD437A90C6730" modified="2019-05-08" version="1">MAAPVDLELKKAFTELQAKVIDTQQKVKLADIQIEQLNRTKKHAHLTDTEIMTLVDETRMYEGVGRMFILQSKGVIHNQLLEKQKIAEEKIKELEQRKSYLERSVKDAEDNIREMLMARRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPL6</accession>
    <name>A0A452GPL6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000003633"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004196.1">
        <property type="protein sequence ID" value="ENSGAGP00000003633.1"/>
        <property type="gene ID" value="ENSGAGG00000002940.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35077">
        <property type="entry name" value="SIMILAR TO AI661453 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35077:SF2">
        <property type="entry name" value="SIMILAR TO AI661453 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="777"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="833"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="892"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="620"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="644"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="664"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="676"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="697"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="740"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="777"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="809"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="845"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="897"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="999"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1035"/>
            <end position="1046"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1120" mass="121434" checksum="2564DD0730CB922C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQM0</accession>
    <name>A0A452GQM0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GQM0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004227"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004958.1">
        <property type="protein sequence ID" value="ENSGAGP00000004227.1"/>
        <property type="gene ID" value="ENSGAGG00000003504.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16594">
        <property type="entry name" value="RING-HC_TRIM7-like_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="81" mass="9037" checksum="151637FAE6343BDC" modified="2019-05-08" version="1">MATDNPVESLQEEAMCPICLEYFKDPVIIECGHNFCRACISQCWEGSDTAASCPQCRETVQQGNLGPNRKLANMVEITKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQQ9</accession>
    <name>A0A452GQQ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Host cell factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">C1 factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQQ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004028"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004700.1">
        <property type="protein sequence ID" value="ENSGAGP00000004028.1"/>
        <property type="gene ID" value="ENSGAGG00000003296.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035097">
        <property type="term" value="C:histone methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000443">
        <property type="entry name" value="host cell factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000259">
        <property type="entry name" value="Host cell factor 1 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000008">
        <property type="entry name" value="host cell factor 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000015">
        <property type="entry name" value="host cell factor 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043536">
        <property type="entry name" value="HCF1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059124">
        <property type="entry name" value="Kelch_HCF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46003">
        <property type="entry name" value="HOST CELL FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46003:SF2">
        <property type="entry name" value="HOST CELL FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13854">
        <property type="entry name" value="Kelch_HCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="562"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="799" mass="88034" checksum="A87E9A9F465A2531" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQY2</accession>
    <name>A0A452GQY2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytokine-inducible SH2-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQY2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004358"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005120.1">
        <property type="protein sequence ID" value="ENSGAGP00000004358.1"/>
        <property type="gene ID" value="ENSGAGG00000003620.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd10718">
        <property type="entry name" value="SH2_CIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000042">
        <property type="entry name" value="Cytokine-inducible SH2-containing protein b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000002">
        <property type="entry name" value="Suppressor of cytokine signaling 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035887">
        <property type="entry name" value="CIS_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF9">
        <property type="entry name" value="CYTOKINE-INDUCIBLE SH2-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="4" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="8">
        <location>
            <begin position="93"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="209"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="258" mass="29589" checksum="8CEF3B298EFF5028" modified="2019-05-08" version="1">MRFSWSWSDMIICVQGPHPLLAEEKIRRQSLRGIAVDLSEQIMQPRSVMVFQGESAPAFMVPAPENNLPRMRDPEEDLLCIAKTFSYLRESGWYWGSITASEAKQHLQKMPEGTFLVRDSTHPSYLFTLSVKTNRGPTNVRIEYADSKFRLDSNCLSKPRILAFPDVVSLIQHYVMSCTLENKNEAPYPPPSPLSSMQKEMVAAAVHLKLIRPLSRKDSVPSLQHLCRLQINKLTEEVDQLPLPRRMGDYLKQYPFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSU8</accession>
    <name>A0A452GSU8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GSU8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005070"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005913.1">
        <property type="protein sequence ID" value="ENSGAGP00000005070.1"/>
        <property type="gene ID" value="ENSGAGG00000004130.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF368">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 2, SUBFAMILY AB, POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="154" mass="17419" checksum="5C91BA5ADFC87A42" modified="2019-05-08" version="1">VLGPFLLICYEDRKNLPYTNDTTLQGFPIPKVKIVHFDMLVFCVISVKEESPRQFSPSRFLAKKGNFVSREAFLPFSTGSRVCLGEQLARSELFIFFANLLRAFTFTLPKGVKKFSTKLILGSTMQPQPYKLCVVPRGPSTLWPSDPGIAEWPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTU1</accession>
    <name>A0A452GTU1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription factor Elf N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTU1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005446"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006339.1">
        <property type="protein sequence ID" value="ENSGAGP00000005446.1"/>
        <property type="gene ID" value="ENSGAGG00000004422.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022084">
        <property type="entry name" value="TF_Elf_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12310">
        <property type="entry name" value="Elf-1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor Elf N-terminal" evidence="7">
        <location>
            <begin position="4"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="91" mass="10063" checksum="F676893D5D4E8FFA" modified="2019-05-08" version="1">MTSAVVDSGGSGLEFDNSEVENQEENKPVSEYPAVIVEPVPSARLEQGYAAQVLVYDDETYLMQDVAEEQEIETESVETEILLEAKSECLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GV34</accession>
    <name>A0A452GV34_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the taxilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GV34"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005618"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006546.1">
        <property type="protein sequence ID" value="ENSGAGP00000005618.1"/>
        <property type="gene ID" value="ENSGAGG00000004556.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000006552.1">
        <property type="protein sequence ID" value="ENSGAGP00000005624.1"/>
        <property type="gene ID" value="ENSGAGG00000004556.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026183">
        <property type="entry name" value="Taxilin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16127:SF10">
        <property type="entry name" value="BETA-TAXILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16127">
        <property type="entry name" value="TAXILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09728">
        <property type="entry name" value="Taxilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="79"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="470"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="551"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="176"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="103"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="473"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="609" mass="69603" checksum="0BF6599A39AB4074" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GV58</accession>
    <name>A0A452GV58_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dUTPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GV58"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005654"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006587.1">
        <property type="protein sequence ID" value="ENSGAGP00000005654.1"/>
        <property type="gene ID" value="ENSGAGG00000004589.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051592">
        <property type="entry name" value="HERV-K_Pro_peptidase_A2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19422">
        <property type="entry name" value="GAG RETROVIRAL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19422:SF123">
        <property type="entry name" value="RT1 CLASS I, LOCUS CE15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase-like" evidence="5">
        <location>
            <begin position="24"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="148" mass="15625" checksum="C7E5D9F0DC181ED7" modified="2019-05-08" version="1">MLQNIELDINNPLRFATQDMLMGAATRGSAGVDLATAADVTLENDKVQVVPSVVNGPLGYGLSALLIGRSSMSKQGIFVLPGLIDADYTGNIGIMVRALFPPVNIAAGTRIAQLIPFHSSVPKTKLTERGPHGFGSTDSPQNNLQRVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GV70</accession>
    <name>A0A452GV70_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial import inner membrane translocase subunit TIM17</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 12">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Mitochondrion inner membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GV70"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006025"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007019.1">
        <property type="protein sequence ID" value="ENSGAGP00000006025.1"/>
        <property type="gene ID" value="ENSGAGG00000004893.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005678">
        <property type="entry name" value="Tim17"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00980">
        <property type="entry name" value="3a0801so1tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10485">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10485:SF2">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM17-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="172" mass="18185" checksum="80CC21F0FF9CB4F4" modified="2019-05-08" version="1">MEEYAREPCPWRIVDDCGGAFTMGMIGGGVFQAVKGFRNAPVGVRHRFKGSVSAIRIRAPQIGGSFAVWGGLFSTIDCGLVKMRGKEDPWNSITSGALTGAVLASRSGPLAMVGSAMMGGILLALIEGVGILLTRYTAQQFQNSNPFGEDPSQLPLKDSPAPPGYPGYGQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GV97</accession>
    <name>A0A452GV97_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glucosaminyl (N-acetyl) transferase 2 (I blood group)</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GV97"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006040"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007034.1">
        <property type="protein sequence ID" value="ENSGAGP00000006040.1"/>
        <property type="gene ID" value="ENSGAGG00000004904.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19297">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 14 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19297:SF183">
        <property type="entry name" value="N-ACETYLLACTOSAMINIDE BETA-1,6-N-ACETYLGLUCOSAMINYL-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="39820" checksum="DEBA3EC52A7C508F" modified="2019-05-08" version="1">MNSRIFCFFAVLVLSVSISLVLYTVNSPEQKHLRKWNLSVTSILAEACDALIEEEVFFVQENTLKTSFGRTICTKYLIQNHYITSPLSAEEAAFPLAYIITLHKEFDTFERLFRAIYMPQNVYCIHVDRKATAKFKQDVEKLLNCFPNAFLASKMELVVYAGISRLQADLNCMKDLLESGVRWKYLLNTCGQDFPLKTNKEIVQYLKRFKGKNITPGGLPPANIIRRTKYVHREQIYSFSSFMLWTFLRKAPPPHNLIIYFGSAYIAVTREFTELVLRDQRAIDLLEWSKDTYSPDEHFWVTLNSIPGMSDFPPTMSSLLTYVILLPNRSAILQTKWIVLFSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GW54</accession>
    <name>A0A452GW54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CutA divalent cation tolerance homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CutA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GW54"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006297"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007319.1">
        <property type="protein sequence ID" value="ENSGAGP00000006297.1"/>
        <property type="gene ID" value="ENSGAGG00000005069.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.120:FF:000011">
        <property type="entry name" value="CutA divalent cation tolerance homolog-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004323">
        <property type="entry name" value="Ion_tolerance_CutA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419:SF2">
        <property type="entry name" value="CUTA DIVALENT CATION TOLERANCE HOMOLOG-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419">
        <property type="entry name" value="DIVALENT CATION TOLERANCE CUTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03091">
        <property type="entry name" value="CutA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="18200" checksum="80F6E22F0398CF66" modified="2019-05-08" version="1">MKMDWLTQRCQLPAAQGCSRPGCFTLLLVITLSLLMYPVLKSLGLQLHSAVTGSYISGTHSIAFINCPNEQVAKDIARAIMDKKLAACVNILPKASSMYFWKGEIEEATEILLLVKTRTSKISELSDYIRSVHPFEIPEFISLPIDQGNPLYLKWIEEGVPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWP7</accession>
    <name>A0A452GWP7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Acyl-coenzyme A oxidase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Oxidizes the CoA-esters of 2-methyl-branched fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2S)-pristanoyl-CoA + O2 = (2E)-pristenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40459"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:77099"/>
            <dbReference type="ChEBI" id="CHEBI:77293"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:40460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:38959"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.3.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:38960"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hexadecanedioyl-CoA + O2 = (2E)-hexadecenedioyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40275"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:77075"/>
            <dbReference type="ChEBI" id="CHEBI:77085"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:40276"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hexadecanoyl-CoA + O2 = (2E)-hexadecenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40167"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:40168"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>tetracosanoyl-CoA + O2 = (2E)-tetracosenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40319"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:74693"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:40320"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; peroxisomal fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GWP7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006538"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007607.1">
        <property type="protein sequence ID" value="ENSGAGP00000006538.1"/>
        <property type="gene ID" value="ENSGAGG00000005297.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016402">
        <property type="term" value="F:pristanoyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01150">
        <property type="entry name" value="AXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000007">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000010">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000005">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034171">
        <property type="entry name" value="ACO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF390">
        <property type="entry name" value="PEROXISOMAL ACYL-COENZYME A OXIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000168">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17 19" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="21">
        <location>
            <begin position="175"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="22">
        <location>
            <begin position="322"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-terminal" evidence="20">
        <location>
            <begin position="532"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="471"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="720" mass="81302" checksum="6F932AC6CC545F8A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWQ8</accession>
    <name>A0A452GWQ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Myosin-binding protein C, slow-type</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">C-protein, skeletal muscle slow isoform</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. Slow skeletal protein that binds to both myosin and actin. In vitro, binds to native thin filaments and modifies the activity of actin-activated myosin ATPase. May modulate muscle contraction or may play a more structural role.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with USP25 (isoform USP25m only); the interaction prevents proteasomal degradation of MYBPC1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the immunoglobulin superfamily. MyBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GWQ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006548"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007619.1">
        <property type="protein sequence ID" value="ENSGAGP00000006548.1"/>
        <property type="gene ID" value="ENSGAGG00000005303.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05894">
        <property type="entry name" value="Ig_C5_MyBP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000286">
        <property type="entry name" value="Myosin binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000326">
        <property type="entry name" value="Myosin-binding protein C, cardiac-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000060">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000062">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000070">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000081">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000085">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000111">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000249">
        <property type="entry name" value="myosin-binding protein C, slow-type isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040849">
        <property type="entry name" value="MyBP-C_THB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF27">
        <property type="entry name" value="MYOSIN-BINDING PROTEIN C, SLOW-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18362">
        <property type="entry name" value="THB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="380"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="472"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="562"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="662"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="762"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="877"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="974"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="1087"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1210" mass="136186" checksum="66BBE18E0268597A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0I4</accession>
    <name>A0A452H0I4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Integrin alpha-X-like third Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000007862"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009062.1">
        <property type="protein sequence ID" value="ENSGAGP00000007862.1"/>
        <property type="gene ID" value="ENSGAGG00000006261.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.930:FF:000004">
        <property type="entry name" value="Integrin subunit alpha M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048633">
        <property type="entry name" value="ITGAX-like_Ig_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF118">
        <property type="entry name" value="INTEGRIN ALPHA-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00357">
        <property type="entry name" value="Integrin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21520">
        <property type="entry name" value="ITGAX-like_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0401">Integrin</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha-X-like third Ig-like" evidence="9">
        <location>
            <begin position="27"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="251" mass="27726" checksum="4C14B5E61574B6FC" modified="2019-05-08" version="1">MTPHHHSPAPSITFSPLSFFPSFSSPSLEEWTKYVSVSTKKAGTSVPVTHRYEVKNLRQRSVPISVTFQFPVELSGVRVWDASEVVPSKVPASPCPCVIAXLLPHPAISFHPQDCSVATCKKIRCRIASLEMQQPLEFMIKGNVSFQWVSQNVSLVSEARIEYEEEKYTQKEGFIQCQVPDSKVYNYLPIIVGSSVGGLVLLALITAALYKLGFFKRQYKQMMEDAVEGEGSCLTQSATAGNPPASDTPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H291</accession>
    <name>A0A452H291_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
            <ecNumber evidence="9">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 17">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EC" id="3.1.3.48" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452H291"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008524"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009811.1">
        <property type="protein sequence ID" value="ENSGAGP00000008524.1"/>
        <property type="gene ID" value="ENSGAGG00000006769.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070373">
        <property type="term" value="P:negative regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060420">
        <property type="term" value="P:regulation of heart growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14566">
        <property type="entry name" value="DSP_MKP_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000011">
        <property type="entry name" value="Dual specificity phosphatase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000011">
        <property type="entry name" value="Dual specificity phosphatase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008343">
        <property type="entry name" value="MKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF45">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000939">
        <property type="entry name" value="MAPK_Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01764">
        <property type="entry name" value="MAPKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="14">
        <location>
            <begin position="30"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="207"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="263"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="175"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="10">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="382" mass="42439" checksum="961607131547D933" modified="2019-05-08" version="1">MLDTFRPVPFVSEMAVSKTVAWLNEQLELGNGRLLLMDCRSQELYESSHIESAISVALPGIMLRRLQKGNLPLRALFSTSDQDREKFARRCGTDTVVLYDENSSEWNENTGGESVLGLLLKRLKDEGCKAFYLEGGFSKFQTEFALHCETNLDSSCSSSSPSLPVLGLGGLRISSDSSSDIESDTDRDPNSATDSDGSPLSNNQPSFPVEILPYLYLGCAKDSTNLDVLEEFGIKYILNVTPNLPNLFENAGEFKYKQIPISDHWSQNLSQFFPEAISFIDEARGKNCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLSSPCDNRVPSQQLYFTSPSNQNVFQVDSRQST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2C2</accession>
    <name>A0A452H2C2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H2C2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008653"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009947.1">
        <property type="protein sequence ID" value="ENSGAGP00000008653.1"/>
        <property type="gene ID" value="ENSGAGG00000006848.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000030">
        <property type="entry name" value="Zinc finger protein 628"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="13">
        <location>
            <begin position="13"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="111"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="416" mass="48194" checksum="3273F33F2992625F" modified="2019-05-08" version="1">IAKPIGEHTEMQRLHFRQFRYQEAKGPREVCSRLRELSHRWLKPEIRTKEQIMELLILEQFLTILPEEIQSWVWVRHPETCAQAVSLAEDFQLGRREVTVRVKVEEVTPEEVETPEALWESQSSQLEPPQPHTTWGSQEEVGRSKYKLPRAPEEETEALQETVEERPSLGEQENLDLSRTSQGVSGESVARRLEQEEACKRQKRNSAGNERIQPRVFPQCRDCGERFWEKQDLMVHGRAHEKDRPYPCPECGKSFNRLTHLRTHQRTHTGVKPYSCGECGKSFGHLSTLTTHQRLHTGERPYSCAECGKTFTNPSDLNKHQRSHTGERPYPCAECGKRFSQQSNLTMHQRSHTEDRPYPCTECGKSFKYLADLTVHERSHTGERPFPCPECGKSFSNKSSLARHQRIHARAAARNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2J9</accession>
    <name>A0A452H2J9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H2J9"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010125.1">
        <property type="protein sequence ID" value="ENSGAGP00000008806.1"/>
        <property type="gene ID" value="ENSGAGG00000006971.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000010129.1">
        <property type="protein sequence ID" value="ENSGAGP00000008809.1"/>
        <property type="gene ID" value="ENSGAGG00000006975.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="53" mass="6021" checksum="B066C21035C7179E" modified="2019-05-08" version="1">PDSPGRSCSKSCSFRGTHLRMDIGPPDVLERKGALTLRSHCVQYGRPTLMYRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3G6</accession>
    <name>A0A452H3G6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 22">Cholesterol side-chain cleavage enzyme, mitochondrial</fullName>
            <ecNumber evidence="4 22">1.14.15.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Cholesterol desmolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>6 reduced [adrenodoxin] + cholesterol + 3 O2 + 6 H(+) = 4-methylpentanal + pregnenolone + 6 oxidized [adrenodoxin] + 4 H2O</text>
            <dbReference type="Rhea" id="RHEA:35739"/>
            <dbReference type="Rhea" id="RHEA-COMP:9998"/>
            <dbReference type="Rhea" id="RHEA-COMP:9999"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:16581"/>
            <dbReference type="ChEBI" id="CHEBI:17998"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.14.15.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20 22">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 22">Lipid metabolism; C21-steroid hormone metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="22">Mitochondrion inner membrane</location>
            <topology evidence="22">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="22">Localizes to the matrix side of the mitochondrion inner membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.6" evidence="4 22"/>
    <dbReference type="AlphaFoldDB" id="A0A452H3G6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009028"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010365.1">
        <property type="protein sequence ID" value="ENSGAGP00000009028.1"/>
        <property type="gene ID" value="ENSGAGG00000007120.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00229"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008386">
        <property type="term" value="F:cholesterol monooxygenase (side-chain-cleaving) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006700">
        <property type="term" value="P:C21-steroid hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20643">
        <property type="entry name" value="CYP11A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000015">
        <property type="entry name" value="Cholesterol side-chain cleavage enzyme, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF3">
        <property type="entry name" value="CHOLESTEROL SIDE-CHAIN CLEAVAGE ENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 22" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="7 20 21" id="KW-0349">Heme</keyword>
    <keyword evidence="12 20 21" id="KW-0408">Iron</keyword>
    <keyword evidence="14 22" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 20 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 22" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13 21" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="11 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18 22" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="19 22" id="KW-0755">Steroidogenesis</keyword>
    <keyword evidence="17 22" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="10 22" id="KW-0809">Transit peptide</keyword>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU364077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="523" mass="60258" checksum="6B22F38748BF3D87" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3K4</accession>
    <name>A0A452H3K4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sodium-coupled monocarboxylate transporter 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Electrogenic sodium monocarboxylate cotransporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Solute carrier family 5 member 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(R)-3-hydroxybutanoate(out) + 2 Na(+)(out) = (R)-3-hydroxybutanoate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72959"/>
            <dbReference type="ChEBI" id="CHEBI:10983"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(S)-lactate(out) + 2 Na(+)(out) = (S)-lactate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72935"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>4-methyl-2-oxopentanoate(out) + 2 Na(+)(out) = 4-methyl-2-oxopentanoate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72967"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>5-oxo-L-proline(out) + 2 Na(+)(out) = 5-oxo-L-proline(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72971"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58402"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>acetate(out) + 2 Na(+)(out) = acetate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72947"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>acetoacetate(out) + 2 Na(+)(out) = acetoacetate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72963"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>bromide(in) = bromide(out)</text>
            <dbReference type="Rhea" id="RHEA:75383"/>
            <dbReference type="ChEBI" id="CHEBI:15858"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>butanoate(out) + 2 Na(+)(out) = butanoate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72951"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>iodide(out) = iodide(in)</text>
            <dbReference type="Rhea" id="RHEA:66324"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>nicotinate(out) + 2 Na(+)(out) = nicotinate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72955"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>nitrate(in) = nitrate(out)</text>
            <dbReference type="Rhea" id="RHEA:34923"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>propanoate(out) + 2 Na(+)(out) = propanoate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72939"/>
            <dbReference type="ChEBI" id="CHEBI:17272"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>pyruvate(out) + 2 Na(+)(out) = pyruvate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72943"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 30">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3K4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009177"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010540.1">
        <property type="protein sequence ID" value="ENSGAGP00000009177.1"/>
        <property type="gene ID" value="ENSGAGG00000007255.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005343">
        <property type="term" value="F:organic acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015730">
        <property type="term" value="P:propanoate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11519">
        <property type="entry name" value="SLC5sbd_SMCT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000007">
        <property type="entry name" value="Sodium-coupled monocarboxylate transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041992">
        <property type="entry name" value="SLC5sbd_SMCT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF10">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 31" id="KW-0472">Membrane</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="6 31" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 31" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="74"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="341"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="412"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="436"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="519"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00035074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00035084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00035085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00035086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00035087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00035088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00035089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00035095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00049528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="613" mass="66724" checksum="2F55876EFD681A5D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4E1</accession>
    <name>A0A452H4E1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Caspase recruitment domain family member 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H4E1"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010748.1">
        <property type="protein sequence ID" value="ENSGAGP00000009351.1"/>
        <property type="gene ID" value="ENSGAGG00000007404.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000088">
        <property type="entry name" value="P53-induced death domain protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025307">
        <property type="entry name" value="FIIND_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021861">
        <property type="entry name" value="THO_THOC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265:SF1">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265">
        <property type="entry name" value="THO COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13553">
        <property type="entry name" value="FIIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23679">
        <property type="entry name" value="UPA-FIIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51830">
        <property type="entry name" value="FIIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FIIND" evidence="5">
        <location>
            <begin position="58"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="4">
        <location>
            <begin position="381"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="338"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="53198" checksum="092EC3772F295B21" modified="2019-05-08" version="1">MCRAESYGTTEDDSAQECKSGEDSSENNSESSSESESDNETDIRKSEANSQFRCEHVKTEHIQKEQVTPRKLSGGQFRLKLEAEGTYQCTVTGLIFDVTKAAVIKYSVLSWSKYADYVKKPWIVGGPIFDVSCDSASVLTSIQFPHSLSLNDHESDMTFKVLHIRSTGPAIEPSVDHSMTHVKWHVSSLSPVGPVIQTQEPVHYNGAVILYKVVNNHPSLSFRVYVATNNDSFIKDISNAVKHSDKKFIKIDKPPVCKKLQDGKRYRLISESEAEITPEEMEFVDGSLLKLKSYFEVYLEQPMEFTLSLIELESEEIVWKAKLRECDWILHDQNKNERKRNTISNRRRKSSNSLSDEEFYNKRPKKGRDISDGTKAKTTLTDQQLMTLAKKMGKDWKEIGIECLKLEMKDIQQIQAKEEEVNVHKFLMLEKWRKGEKSNGTAQNLYDSLKDHVSYEIIQILEGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8D3</accession>
    <name>A0A452H8D3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pectinacetylesterase family. Notum subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H8D3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010984"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012578.1">
        <property type="protein sequence ID" value="ENSGAGP00000010984.1"/>
        <property type="gene ID" value="ENSGAGG00000008509.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990699">
        <property type="term" value="F:palmitoleyl hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF7">
        <property type="entry name" value="PALMITOLEOYL-PROTEIN CARBOXYLESTERASE NOTUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019454397" evidence="2">
        <location>
            <begin position="24"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="498" mass="55983" checksum="E3F162E269278EA7" modified="2019-05-08" version="1">MGRGTVQVLLLLGLLHWGPGGEGRKNWRRRGQQPAAAAAARERSEATGQPVESFPLDFTAVEGNMDSFMAQIKSLAQSLYPCSAQNLHHELRLHLLLNSSVTCNDGSPAGYYLKESKGSRRWLLFLEGGWYCFNRENCDTRYDTMRRLMSSKEWPITKTGTGILSSQPEENPHWWNANMVFIPYCSSDVWSGASSKSEKNEYAFMGALIIQEVVKELVGKGLSNAKVLLLAGSSAGGTGVLLNVDRVAEQLEEMGYQGIQVRGLADSGWFLDNKQYRRTDCIDTITCAPTEAIRRGIRYWNGIVPERCKLQFKEGEEWNCFFGYKIYPTLRCPVFVVQWLFDEAQLTVDNVHLTGQPVQEGQWLYIQNLGRELRNTLKDVTASFAPACLSHEIITRNHWTDIQVKGTSLPRALHCWDRSLHDSNKNGKAPLKGCPIHLIDSCPWPHCNPSCPTIRDQFTGQEMNVIQFLMHMGFDVQKMAQQQGLEPSKLLGMLSSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HB05</accession>
    <name>A0A452HB05_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Neurolysin, mitochondrial</fullName>
            <ecNumber evidence="14">3.4.24.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Microsomal endopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Mitochondrial oligopeptidase M</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Neurotensin endopeptidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A. Acts as a regulator of cannabinoid signaling pathway by mediating degradation of hemopressin, an antagonist peptide of the cannabinoid receptor CNR1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Preferential cleavage in neurotensin: 10-Pro-|-Tyr-11.</text>
            <dbReference type="EC" id="3.4.24.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="20">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.16" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A452HB05"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011919"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013652.1">
        <property type="protein sequence ID" value="ENSGAGP00000011919.1"/>
        <property type="gene ID" value="ENSGAGG00000009189.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06455">
        <property type="entry name" value="M3A_TOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.40:FF:000001">
        <property type="entry name" value="Thimet oligopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000006">
        <property type="entry name" value="Thimet oligopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.40">
        <property type="entry name" value="Endopeptidase. Chain P, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024080">
        <property type="entry name" value="Neurolysin/TOP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF44">
        <property type="entry name" value="NEUROLYSIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 20" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 20" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8 20" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="21">
        <location>
            <begin position="189"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00046222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="527" mass="61147" checksum="0175AD8C0EE712BF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HB72</accession>
    <name>A0A452HB72_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mab-21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HB72"/>
    <dbReference type="SMR" id="A0A452HB72"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012005"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013748.1">
        <property type="protein sequence ID" value="ENSGAGP00000012005.1"/>
        <property type="gene ID" value="ENSGAGG00000009248.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001654">
        <property type="term" value="P:eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.40:FF:000002">
        <property type="entry name" value="protein mab-21-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.90:FF:000001">
        <property type="entry name" value="protein mab-21-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046903">
        <property type="entry name" value="Mab-21-like_nuc_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046906">
        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF37">
        <property type="entry name" value="PROTEIN MAB-21-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03281">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20266">
        <property type="entry name" value="Mab-21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mab-21-like nucleotidyltransferase" evidence="7">
        <location>
            <begin position="62"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Mab-21-like HhH/H2TH-like" evidence="8">
        <location>
            <begin position="246"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="359" mass="40907" checksum="24B7A0CF0E78A1AA" modified="2019-05-08" version="1">MIAAQAKLVYQLNKYYTERCQARKAAIAKTIREVCKVVSDVLKEVEVQEPRFISSLSEIDARYEGLEVISPTEFEVVLYLNQMGVFNFVDDGSLPGCAVLKLSDGRKRSMSLWVEFITASGYLSARKIRSRFQTLVAQAVDKCSYRDVVKMIADTSEVKLRIRERYVVQITPAFKCTGIWPRSAAQWPMPHIPWPGPNRVAEVKAEGFNLLSKECYSLTGKQSSAESDAWVLQFGEAENRLLMGGCRNKCLSVLKTLRDRHLELPGQPLNNYHMKTLLLYECEKHPRETDWDEACLGDRLNGILLQLISCLQCRRCPHYFLPNLDLFQGKPHSALESAAKQTWRLAREILTNPKSLDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBP3</accession>
    <name>A0A452HBP3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HBP3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012180"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013950.1">
        <property type="protein sequence ID" value="ENSGAGP00000012180.1"/>
        <property type="gene ID" value="ENSGAGG00000009375.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029638">
        <property type="entry name" value="FAM78"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31655">
        <property type="entry name" value="PROTEIN FAM78A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31655:SF0">
        <property type="entry name" value="PROTEIN FAM78B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="237" mass="27257" checksum="371551355CEBADDB" modified="2019-05-08" version="1">TWSRSLRLRFSKLTRGFSRTFPVTIKLMEVNGKGDVMQQGFIKDRSCQTNFFVYNLVLSFSFPRSSWELPDLREGRVKAISDSDGVSYPWYGNTTETVTLVGPTNKVSRFSVSMNDNFYPSVTWAVPVSDSNVPLLTRIKRDQSFTTWLVAMNMTTKEKIILQTIKWRMRVDIEVDPLQLLGQRARLVGRTHQEQPRILSRMEPIPPNALVKPNANDAQVLMWRPKRGQPLIVIPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HD36</accession>
    <name>A0A452HD36_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Laforin</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
            <ecNumber evidence="5">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Glucan phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Lafora PTPase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 29">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="EC" id="3.1.3.48" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452HD36"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012708"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014569.1">
        <property type="protein sequence ID" value="ENSGAGP00000012708.1"/>
        <property type="gene ID" value="ENSGAGG00000009752.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019203">
        <property type="term" value="F:carbohydrate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009892">
        <property type="term" value="P:negative regulation of metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05806">
        <property type="entry name" value="CBM20_laforin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14526">
        <property type="entry name" value="DSP_laforin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001039">
        <property type="entry name" value="laforin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000054">
        <property type="entry name" value="laforin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034831">
        <property type="entry name" value="CBM20_laforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045204">
        <property type="entry name" value="DSP_laforin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042942">
        <property type="entry name" value="Laforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46864">
        <property type="entry name" value="LAFORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46864:SF1">
        <property type="entry name" value="LAFORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0072">Autophagy</keyword>
    <keyword evidence="17" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="23" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019096698" description="Laforin" evidence="23">
        <location>
            <begin position="17"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="CBM20" evidence="26">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="24">
        <location>
            <begin position="143"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="25">
        <location>
            <begin position="244"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00072874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00079550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="317" mass="35808" checksum="CA17847BBFAEFD46" modified="2019-05-08" version="1">MLFRFGVVLPAGLAEGGVVLLVAGSRPELGHWDPQRALPMRPARPAAPLPAQEPALWLAEAALPDEEAAGTFWYKFLQRRGADTIWEGNGPHHDRCCVYNESNLVDGVYCLPIGHWIEVSGHTDEMKHTTDFYFSIAGHQAIHYSRILPNIWLGSCPRQVEHVTIKLKHELGVTAVMNFQTEWDVVQNSWGCNRYPEPMSPEVLMKLYKEEGLAYVWMPTPDMSTEGRIQMLPQAVCLLHGLLENGHIVYVHCNAGVGRSTAAVCGWLKYVMGWNLRKVQYYVASRRPAVYIDEEALARAEDDFYQKFGPLRYSCKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDR2</accession>
    <name>A0A452HDR2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HDR2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012981"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014876.1">
        <property type="protein sequence ID" value="ENSGAGP00000012981.1"/>
        <property type="gene ID" value="ENSGAGG00000009966.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000269">
        <property type="entry name" value="Methyltransferase-like protein 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052356">
        <property type="entry name" value="Thiol_S-MT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45036:SF1">
        <property type="entry name" value="METHYLTRANSFERASE LIKE 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45036">
        <property type="entry name" value="METHYLTRANSFERASE LIKE 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase type 11" evidence="2">
        <location>
            <begin position="114"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="283" mass="32401" checksum="99507C56E60BCBD1" modified="2019-05-08" version="1">MSQLARRARRKCSVKLEHSSRFGVLFTAGIEDFCILLFALMVLIPLLQRCIQLLSLPVYLLSYLGLWDPLCKKIFPYVMAKCSTIYNHKLFQQKKDLFSSLGEFAGPSMELRLLEIGTGSGANFQFYPPGCRVTCTDPNPNFERFLLKSISDSPHLHFERFLVASGEDLRPVADDSMDVVVCTLVLCSVRSIEQVLREVLRVLRLGGALYFLEHVAADHSSWGYFWQQIYNPTWRYLGDGCCLTRETWKDLEKAGFSELKLRHIRAPLSWNPTRPHIIGYAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HER0</accession>
    <name>A0A452HER0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HER0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013343"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015271.1">
        <property type="protein sequence ID" value="ENSGAGP00000013343.1"/>
        <property type="gene ID" value="ENSGAGG00000010209.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF63">
        <property type="entry name" value="KINESIN FAMILY MEMBER 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="7">
        <location>
            <begin position="99"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="195"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="481" mass="54222" checksum="EBAFF2EF9ED5A0C7" modified="2019-05-08" version="1">MSSVIIQVYGAIQRLKQDLKNLHSFALELSRDFQHQCKNSLYQVLTAVQRIQLQNEAMHTFQTKAFDLEQSLQEVNERYQQEKQKRKILHNSLVELRGNIRVHCRIRPLLPFDTASGHSVSQDRHRKLSEEVAHATDDETVLVKCNRPGHTLINKTFQFDRVYSAAESQDTVFADVLPLLTSLLDGYNVCIMAYGQTGSGKTYTMLGPQSEDDVTLSIEDEPELGIIPRATEEVFRIISEKPPGSHWVEVSVVEVYNNEIFDLLAKDSCGRVFGVKRDVVTTKEGKSDVPLLTYETVENAVEFLQLVDKGLQLRAQHPTLVHAHSSRSHLVVTLTITTKAVSADSFAPLWTDEQSSPRLSKELFCTFPQQMKDDKLIFPSRASSPAQFESTEKLKHIKTKLQLVDLAGSECVGMSGVTGVALRETSFINRSLSALSDVLGAIAEQRSHIPYRNSKLTHLLQDSIGDYFFCLLCNNIELHFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HES1</accession>
    <name>A0A452HES1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">THD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tumor necrosis factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HES1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013307"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015234.1">
        <property type="protein sequence ID" value="ENSGAGP00000013307.1"/>
        <property type="gene ID" value="ENSGAGG00000010195.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00184">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006052">
        <property type="entry name" value="TNF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11471">
        <property type="entry name" value="TUMOR NECROSIS FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11471:SF34">
        <property type="entry name" value="TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00229">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00207">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50049">
        <property type="entry name" value="THD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="THD" evidence="6">
        <location>
            <begin position="94"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="238" mass="27066" checksum="3EB33DC611478773" modified="2019-05-08" version="1">MGDGVVYPSVFVVDGQQHGVPFVPPMRRKRRMCWHSQFFLACLVILALAGVATEGYFLIRFQKELEKATSQVAEEAAAISEKSIQDRKLHAEKPAAHVLGAAFTTSGNGSLQWEHSKDWAFLHDIGYQGGSLVCIKPGYYYIYSKIHLMAQSCSQQGQSSVIYHGIYKKTPRYVEEMKLVENLILYCDEKDSGFWRQNSFLGGIFHLEGEEQIYVKVSQRQMLQIRDGTRSYFGTFMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFK8</accession>
    <name>A0A452HFK8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HFK8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013621"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015592.1">
        <property type="protein sequence ID" value="ENSGAGP00000013621.1"/>
        <property type="gene ID" value="ENSGAGG00000010368.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12635">
        <property type="entry name" value="RRM2_CELF3_4_5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12639">
        <property type="entry name" value="RRM3_CELF3_4_5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000007">
        <property type="entry name" value="CUGBP Elav-like family member 4 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000069">
        <property type="entry name" value="CUGBP Elav-like family member 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="124"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="390"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="475" mass="51201" checksum="FFE19894DFD040D6" modified="2019-05-08" version="1">ALVYHAARPISCEPDISAALPGADNAGLRQTSPPEPAVPPPDSMAAVWLNQFELPTWLSQIKARLERVYEESVCSQPRPWPIGSVQHTPPACVFVMPEHLPTSLPQCACLFRWLTMFGLVSEDRKLFVGMLGKQQSEDDVRRLFEPFGQIEECTILRGPDGASKGCAFVKYGSHGEAQAAINSLHGSQTMPGASSSLVVKFADTDKERTLRRMHQMAGQLGIFNPMTIQFGAYGAYTQAIMQQQAALMAAAQGTCLNPMAAIAAAQMQQMAAFNVSGLVAAPMTPSSGTSTPPGISTAPVPSIATPIGVNGFSPLPPQTNGQPTSETIYTNGIHPYPAQSPTVADPLQQAYAGMQHYAAAYPAAYAPISQAFPQQAPIIPQQQREGPEGCNLFIYHLPQEFGDAELMQMFLPFGNVISAKVFVDRATNQSKCFGFVSFDNPTSAQAAIQAMNGFQIGMKRLKVQLKRPKDANRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGJ8</accession>
    <name>A0A452HGJ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGJ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013987"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016007.1">
        <property type="protein sequence ID" value="ENSGAGP00000013987.1"/>
        <property type="gene ID" value="ENSGAGG00000010647.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000001">
        <property type="entry name" value="semaphorin-5A isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054019">
        <property type="entry name" value="CFP_TSR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052065">
        <property type="entry name" value="Compl_asym_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22906">
        <property type="entry name" value="PROPERDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22906:SF43">
        <property type="entry name" value="PROPERDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22195">
        <property type="entry name" value="TSP1_CFP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="146"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="460" mass="49646" checksum="013256248718A8EB" modified="2019-05-08" version="1">LHAGAPSLCPPRSQAEWSDWTAWGPCSVSCSEGVQRRSQTCLGQGQCSDGSRKRWEIQACSLKDCCPVMGGWSAWGPWSSCSVTCAKGVRKRIRSCTEPTPICGGSCPGPGTDTQPCDTNQICPTHGNWGSWGQWGPCSATCSPEGAEQKPQQRRRRVCNNPPPSSIPPGRPCSGSTSEDQSCPGLPFCPVDGAWSNWETSSPCAVTCGLGRVMQKRLCNNPAPRHGGKACLGLNTRSHFCNTKIPCPVDGQWSEWGEWSTCSRPGYTGSISCREIVGQQRRTRECKGRSPDGKRCAGSYIHIRSCYNMYLCKLEGQWSDWSSWGLCVPPCGKNPVKSRKRVCQPTYPKFSMVTQGVAATGPVNVSFWGKPWPQCKPINGNSLEVEEKEPCLNVPPCPEEEEEYGELGGISSGSECVCVGVLGARTPEFSPLLWEGHGISTGSLWKDPCGAGGLAEVLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGK2</accession>
    <name>A0A452HGK2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="35">Chloride intracellular channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="37 38">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="38" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="37" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="37" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="34">In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Can insert into membranes and form voltage-dependent multi-ion conductive channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Promotes cell-surface expression of HRH3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>bromide(in) = bromide(out)</text>
            <dbReference type="Rhea" id="RHEA:75383"/>
            <dbReference type="ChEBI" id="CHEBI:15858"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>choline(out) = choline(in)</text>
            <dbReference type="Rhea" id="RHEA:32751"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>iodide(out) = iodide(in)</text>
            <dbReference type="Rhea" id="RHEA:66324"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>nitrate(in) = nitrate(out)</text>
            <dbReference type="Rhea" id="RHEA:34923"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>thiocyanate(in) = thiocyanate(out)</text>
            <dbReference type="Rhea" id="RHEA:75347"/>
            <dbReference type="ChEBI" id="CHEBI:18022"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
            <location evidence="5">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="32">Cytoplasmic vesicle membrane</location>
            <topology evidence="32">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="35">Membrane</location>
            <topology evidence="35">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="35">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="35">Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 35">Belongs to the chloride channel CLIC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGK2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013995"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016016.1">
        <property type="protein sequence ID" value="ENSGAGP00000013995.1"/>
        <property type="gene ID" value="ENSGAGG00000010656.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10296">
        <property type="entry name" value="GST_C_CLIC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03061">
        <property type="entry name" value="GST_N_CLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000001">
        <property type="entry name" value="Chloride intracellular channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000021">
        <property type="entry name" value="Chloride intracellular channel 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002946">
        <property type="entry name" value="CLIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053823">
        <property type="entry name" value="CLIC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00862">
        <property type="entry name" value="O-ClC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45476:SF5">
        <property type="entry name" value="CHLORIDE INTRACELLULAR CHANNEL 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45476">
        <property type="entry name" value="CHLORIDE INTRACELLULAR CHANNEL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22441">
        <property type="entry name" value="CLIC-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01263">
        <property type="entry name" value="INTCLCHANNEL"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="22 35" id="KW-0868">Chloride</keyword>
    <keyword evidence="20 35" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9 35" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="25" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="21" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="24 35" id="KW-0407">Ion channel</keyword>
    <keyword evidence="17 35" id="KW-0406">Ion transport</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="38" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 35" id="KW-0813">Transport</keyword>
    <keyword evidence="12 35" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="GST C-terminal" evidence="36">
        <location>
            <begin position="89"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00024145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00035073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00035085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00036811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00036895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00037855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00044702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00045955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="RuleBase" id="RU362009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="261" mass="29420" checksum="A62B729A57C1C035" modified="2019-05-08" version="1">MPTFLLHLRGNACRAGDNSRQGTLCSLQLKRKAGSDGESIGNCPFSQRLFMILWLKGVVFSVTTVDLKRKPADLQNLAPGTHPPFITYNGEVKTDVNKIEEFLEDVLCPPKYLKLSPKHPESNTAGMDIFAKFSAFIKNSRPEANEALERSLLKTLQKLDEYLNTPLPDEIDENSMEDIAASTRKFLDGNEMTLADCNLLPKLHIVKVVAKKYRNFDIPKEMAGIWRYLTNAYSRDEFTNTCPGDKEIEIAYSDVAKRLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIE3</accession>
    <name>A0A452HIE3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000014633"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016735.1">
        <property type="protein sequence ID" value="ENSGAGP00000014633.1"/>
        <property type="gene ID" value="ENSGAGG00000011103.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000002">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="13">
        <location>
            <begin position="46"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="14">
        <location>
            <begin position="246"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="447" mass="49847" checksum="5FBF6496527A6719" modified="2019-05-08" version="1">METVHVGQAGVQMGNTCWELYCLEHGIQPDGQMPSEKTIGGGDDSFTTFFCETGAGKHVPRAIFVDLEPTVIDEIRTGIYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDQVLDRXQLLTADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNSCFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTAVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGLDSYEDEEEGEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIX7</accession>
    <name>A0A452HIX7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HIX7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014853"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016971.1">
        <property type="protein sequence ID" value="ENSGAGP00000014853.1"/>
        <property type="gene ID" value="ENSGAGG00000011246.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000032">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595:SF1">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="7"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="197"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="458"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="579"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="677" mass="77225" checksum="B1247C8F36909030" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJ15</accession>
    <name>A0A452HJ15_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUP186/NUP192/NUP205 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000014897"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017016.1">
        <property type="protein sequence ID" value="ENSGAGP00000014897.1"/>
        <property type="gene ID" value="ENSGAGG00000011265.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021827">
        <property type="entry name" value="Nup186/Nup192/Nup205"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11894">
        <property type="entry name" value="Nup192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2032" mass="230988" checksum="9EC5258967E89F98" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKE3</accession>
    <name>A0A452HKE3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HKE3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015389"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017576.1">
        <property type="protein sequence ID" value="ENSGAGP00000015389.1"/>
        <property type="gene ID" value="ENSGAGG00000011610.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000231">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF351">
        <property type="entry name" value="SYNAPTOTAGMIN-4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019305158" description="C2 domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="160"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="311"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="393" mass="43925" checksum="9E8A804238AA6497" modified="2019-05-08" version="1">MLLGVGLALLCFCLLLGCALCWRRNERHNKSNRKEQAPTNTLVELGPALPSQTATVPIQQQYVELEGEVLECAGNDSLDSPGAGSLPRSMLHGRASLPSIPFSQKLGLFSKTEPGWERRSTIFGETDKSSLLTRPVLGASIRQSSTMPGSLASASASPKQRPQLHFILFYSQPEATLTVTVISVSHLPKGFRSSRDSYVKVYLLPKFIKPQRTAVCRKSLNPEFRAQFQFGRYHLEELRGFTLRFAVYVKEFRSFRDSFIGEVTFPCAQVTWNTEAASSYTRELSATKTKLKKCLSTQDMSYGAACSQSTSLGQLFILLQYQVLANRIKVLVRKAENLGRLTRMPGTPDHYVVIHFYYDGRVMDTKETKSIAGYNPVWNKPFLFSVPAGDIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKE8</accession>
    <name>A0A452HKE8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IRG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000015394"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017585.1">
        <property type="protein sequence ID" value="ENSGAGP00000015394.1"/>
        <property type="gene ID" value="ENSGAGG00000011616.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000541">
        <property type="entry name" value="Immunity related GTPase M"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051515">
        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341:SF17">
        <property type="entry name" value="IRG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1218"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="domain" description="IRG-type G" evidence="6">
        <location>
            <begin position="56"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="IRG-type G" evidence="6">
        <location>
            <begin position="461"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="IRG-type G" evidence="6">
        <location>
            <begin position="873"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1247" mass="140431" checksum="CB061DC553D6E665" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HN30</accession>
    <name>A0A452HN30_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Regulator of cell cycle</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HN30"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018669.1">
        <property type="protein sequence ID" value="ENSGAGP00000016359.1"/>
        <property type="gene ID" value="ENSGAGG00000012254.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029252">
        <property type="entry name" value="RGCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32193">
        <property type="entry name" value="REGULATOR OF CELL CYCLE RGCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32193:SF5">
        <property type="entry name" value="RGCC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15151">
        <property type="entry name" value="RGCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12246" checksum="67E11F930AC2D5B4" modified="2019-05-08" version="1">MENMNNAVLPDDLNDLLREFDEVIEDFDKGEVCQYEQHLEELKRRTLPSVYDSGIDELESTSTSPGSSLNSSEEDLNTSASIANSKAKLGDTQELEEFIADLDKVLEEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HPB7</accession>
    <name>A0A452HPB7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">5' exonuclease Apollo</fullName>
            <ecNumber evidence="5">3.5.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">DNA cross-link repair 1B protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">SNM1 homolog B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a beta-lactam + H2O = a substituted beta-amino acid</text>
            <dbReference type="Rhea" id="RHEA:20401"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35627"/>
            <dbReference type="ChEBI" id="CHEBI:140347"/>
            <dbReference type="EC" id="3.5.2.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.6" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452HPB7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016826"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019210.1">
        <property type="protein sequence ID" value="ENSGAGP00000016826.1"/>
        <property type="gene ID" value="ENSGAGG00000012571.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12650:FF:000003">
        <property type="entry name" value="DNA cross-link repair 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF26">
        <property type="entry name" value="5' EXONUCLEASE APOLLO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="18">
        <location>
            <begin position="156"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="229"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="327"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="476" mass="53274" checksum="7DD3DA68CB069FEA" modified="2019-05-08" version="1">MTVTLIDANHCPGSVMFLFEGPFGVILYTGDFRYTPTMLQEPALRSRKQIDVLYLDNTNCDPLGDLPSRQQATEQIKELIRAHPDYEVKIGVYSLGKESLLVDLALEFQTWIVVSPKRLEQMKVLGLVDVFTAEKGAGWIHGVDISEICWEAMISWNQQRPTIAILPTSRPVKIPHRSVHLVPYSDHSSFSELLEFVQWLKPCSIVPIVKRNVCLPYFEKYLSSDHKALPEPRIPESGQKFTQLRESREQQRATQQPVPRKDSFASPEKHTNWLEHFGDVTISQQSSSEQPRDPNTHSQGSFVCCRGERKGIKVELCQLHGSQRQLFSAQPNTQTPARTQPLPPKDTGARTSHSSTKDAAQPHTSTLFWQTGKKHTSPCQPPLGDGAPLTLASVQKKAALPSHKQSLTCLQTEKFLPPGAAGDGVTLASWSPVASSHVACGLAEEYLLAPLNTLKQYSAESFNWQIENYFRREDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQP2</accession>
    <name>A0A452HQP2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HQP2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017323"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019766.1">
        <property type="protein sequence ID" value="ENSGAGP00000017323.1"/>
        <property type="gene ID" value="ENSGAGG00000012901.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022179">
        <property type="entry name" value="CFAP276"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12494">
        <property type="entry name" value="DUF3695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="267" mass="29695" checksum="97EB25C5D561DE52" modified="2019-05-08" version="1">MPEFLCGKTAGKDAQSFHAQPSAAPCIALPATACRSRHLPPPPCGPPRRAALSSHQGEPQRQSCLLGIPGAGTVAWQPRQGSQTSGSERCCARRRMPPTRDPFPYPRYENEDTFTGQARPGQIGPYTEPTHLAQQQDPWNRLHRTPTLSSTRKEVWYTEPEVPRDSLDFKLTSLYNHHKEQLRDKSEIVIHKETLLDDHGALKGLEPVKEPEPPPMEHRTARGLSVRCWVSPTKESIHSIEGAIECPHTAATNSGYSRKENGGFFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRE7</accession>
    <name>A0A452HRE7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-sulfur cluster assembly 1 homolog, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HRE7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017576"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020049.1">
        <property type="protein sequence ID" value="ENSGAGP00000017576.1"/>
        <property type="gene ID" value="ENSGAGG00000013054.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.300.12:FF:000001">
        <property type="entry name" value="Iron-binding protein IscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050322">
        <property type="entry name" value="Fe-S_cluster_asmbl/transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072:SF41">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Core" evidence="4">
        <location>
            <begin position="49"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="130" mass="14262" checksum="32CA07616C80DBF6" modified="2019-05-08" version="1">FAPASTMPKRKSVSYLLIRATLTTASLTFCLSHTQLQPVKSLVPVFVGVKVGVRTRGCNGLSYTLDYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYVEDKLSSEFVFNNPNIKGTCGCGESFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTE6</accession>
    <name>A0A452HTE6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">small monomeric GTPase</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452HTE6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018363"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020928.1">
        <property type="protein sequence ID" value="ENSGAGP00000018363.1"/>
        <property type="gene ID" value="ENSGAGG00000013598.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04139">
        <property type="entry name" value="RalA_RalB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000203">
        <property type="entry name" value="Putative ras-related protein ral-a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="180" mass="20365" checksum="13080AC9EC0FDA79" modified="2019-05-08" version="1">MAANKPKSQNSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITELESFAATADFREQILRVKEDENVPFLLVGNKSDLEDKRQVSVEEAKNRADQWNVNYVETSAKTRANVDKVMTFKYKILKTKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTG4</accession>
    <name>A0A452HTG4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein-lysine N-methyltransferase SMYD4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">SET and MYND domain-containing protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Protein-lysine N-methyltransferase. Monomethylates PRMT5, modulating its transcriptional activity. May also act as a histone methyltransferase. Plays a critical role in cardiac development. Acts as a key epigenetic regulator of gene expression during cardiac development via its dual activities as a methyltransferase and negative regulator of HDAC1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-lysyl-[protein] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51736"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HTG4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018383"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020951.1">
        <property type="protein sequence ID" value="ENSGAGP00000018383.1"/>
        <property type="gene ID" value="ENSGAGG00000013610.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10536">
        <property type="entry name" value="SET_SMYD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052097">
        <property type="entry name" value="SET-MYND_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044421">
        <property type="entry name" value="SMYD4_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46165">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46165:SF2">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="17">
        <location>
            <begin position="230"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="18">
        <location>
            <begin position="293"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="803" mass="89331" checksum="759235E26CF9CEC4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTI9</accession>
    <name>A0A452HTI9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protocadherin-19</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HTI9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018434"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021009.1">
        <property type="protein sequence ID" value="ENSGAGP00000018434.1"/>
        <property type="gene ID" value="ENSGAGG00000013643.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000360">
        <property type="entry name" value="Protocadherin 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000088">
        <property type="entry name" value="protocadherin-19 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000099">
        <property type="entry name" value="protocadherin-19 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF40">
        <property type="entry name" value="PROTOCADHERIN-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019519685" description="Protocadherin-19" evidence="17">
        <location>
            <begin position="19"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="676"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="25"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="127"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="236"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="347"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="451"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="566"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="851"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="856"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1095" mass="120377" checksum="966F139716523738" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUD2</accession>
    <name>A0A452HUD2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HUD2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018720"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021341.1">
        <property type="protein sequence ID" value="ENSGAGP00000018720.1"/>
        <property type="gene ID" value="ENSGAGG00000013851.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000292">
        <property type="entry name" value="zinc finger protein 619"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000028">
        <property type="entry name" value="zinc finger protein 90 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="390"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="27"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="56"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="421" mass="48569" checksum="67D6048E658510BF" modified="2019-05-08" version="1">WATVAVSPHLCDEVIKNAGIRNTEFLNEEQNSQPENVEPVGKDRALSQRSKRNVSRSHEQGKSWEIHHRPETDQGNQPGEKVDKFISSWGTQKVLMEATTQQDLLRRKRKNTCSGCGKTSRTSALSVHQRIYTQERPYKCSECGKTFSRSSHLISHQTIHRGERPYECSECGKNFTRRSALSVHRRIHTGERPYECSDCGKSFISISHFSKHQGIHTDERPYECSDCGKTFSRRSALSVHLRIHTGERPYECSDCGKSFNRSSHLIRHQKIHRGERPFECHECGKNFTHRSALSRHQRIHTGERPYVCSECGKNFTERATLSVHQRIHTGERPYECRECGKCFTNSSNLSKHRRIHTGEKPYECSECGKSFTHRSTLSEHQRIHTGERPYECSECGKTFSWHSAHVSHQRICKGDQHCKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUT9</accession>
    <name>A0A452HUT9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Peroxisomal membrane protein PEX16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the peroxin-16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HUT9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018873"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021516.1">
        <property type="protein sequence ID" value="ENSGAGP00000018873.1"/>
        <property type="gene ID" value="ENSGAGG00000013971.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013919">
        <property type="entry name" value="Pex16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13299">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13299:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08610">
        <property type="entry name" value="Pex16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3 4" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="151" mass="17145" checksum="47AB57CB579C9AD2" modified="2019-05-08" version="1">MLMYWMLTKGIVCIRNLVCNSVSAPALQSRHWGSPQQREGKQNRRAEEMNQAPTPLGLQETIAESVYITRPLFHCILVEVFAEMGAAKVWGEMGRWTIIILIQLAKAILRLLLLLWYKAGIQTSPPIVPVDREQQQLQPEGESIALNFLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYL3</accession>
    <name>A0A452HYL3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LIM interaction domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the LDB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYL3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020235"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023054.1">
        <property type="protein sequence ID" value="ENSGAGP00000020235.1"/>
        <property type="gene ID" value="ENSGAGG00000014924.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030274">
        <property type="term" value="F:LIM domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000063">
        <property type="entry name" value="LIM domain-binding protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041363">
        <property type="entry name" value="LID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029005">
        <property type="entry name" value="LIM-bd/SEUSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10378">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17916">
        <property type="entry name" value="LID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01803">
        <property type="entry name" value="LIM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51957">
        <property type="entry name" value="LID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LIM interaction" evidence="7">
        <location>
            <begin position="336"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="284"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="367"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="302"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="411" mass="46606" checksum="37779E0958E1EF9D" modified="2019-05-08" version="1">MSVGCACPGCSSKSFKLYSPKEPPNGNAFPPFHPGTMLDRDVGPTPMYPPTYLEPGIGRHTPYGNQTDYRIFELNKRLQNWTEECDNLWWDAFTTEFFEDDAMLTITFCLEDGPKRYTIGRTLIPRYFRSIFEGGATELYYVLKHPKESFHNNFVSLDCDQCTMVTQHGKPMFTQVCVEGRLYLEFMFDDMMRIKTWHFSIRQHRELIPRSILAMHAQDPQMLDQLSKNITRCGLSNSTLNYLRLCVILEPMQELMSRHKTYSLSPRDCLKTCLFQKWQRMVAPPAEPARQQPSKRRKRKMSGGSTMSSSGGNANNSNSKKKSPASTFALSSQVPDVMVVGEPTLMGGEFGDEDERLITRLENTQFDAANGIDDEDSFNNSPALGANSPWNSKPPSSQESKSENPTSQASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZG7</accession>
    <name>A0A452HZG7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZG7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020579"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023437.1">
        <property type="protein sequence ID" value="ENSGAGP00000020579.1"/>
        <property type="gene ID" value="ENSGAGG00000015149.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15911">
        <property type="entry name" value="7tmA_OR11A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="44"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="319" mass="35933" checksum="3E05B6E1776CAE76" modified="2019-05-08" version="1">MANREWGNETAVTQFILLGFRNIPQLQILVFLLFLMIYIVTMAGNILIIVLVVADQHLHTPMYFFLGNLSCLETCYTSTILPKVLASLLTGDRTISVSGCIVQFYFASFFAATECYLLAVMSYDRYLAICKPLHYATLMNGRFCRQLAAGSWINGCLAIAIITCLMLQLNFCGPNEIDHFFCESTQIINLCCTDTYLIDLVITILVGLFTLPPFALTVVSYVCIISTILTIPSTTGRQKTFSTCSSHLIVVTMFYGTLMIVYLLPKTNTLRDLNKVFSVFYTILTPMTNPFIYSLRNKKVKEALRKIVCKCADFTRIPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I160</accession>
    <name>A0A452I160_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Adenylate cyclase type 9</fullName>
            <ecNumber evidence="5">4.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">ATP pyrophosphate-lyase 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Adenylate cyclase type IX</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Adenylyl cyclase 9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021219"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024165.1">
        <property type="protein sequence ID" value="ENSGAGP00000021219.1"/>
        <property type="gene ID" value="ENSGAGG00000015586.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000024167.1">
        <property type="protein sequence ID" value="ENSGAGP00000021220.1"/>
        <property type="gene ID" value="ENSGAGG00000015586.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000008">
        <property type="entry name" value="Adenylate cyclase type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000014">
        <property type="entry name" value="adenylate cyclase type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF8">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18 23" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 25" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="784"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="817"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="862"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="888"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="918"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="974"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="26">
        <location>
            <begin position="396"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="26">
        <location>
            <begin position="1054"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="351"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="647"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1281"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="24">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="24">
        <location>
            <begin position="361"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="1281"/>
            <end position="1294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00070496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00081232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00081427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1348" mass="151224" checksum="2084323B4664707F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1U1</accession>
    <name>A0A452I1U1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phospholipid phosphatase-related protein type 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Brain-specific phosphatidic acid phosphatase-like protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Inactive 2-lysophosphatidate phosphatase PLPPR4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Lipid phosphate phosphatase-related protein type 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Plasticity-related gene 1 protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Postsynaptic density membrane protein that indirectly regulates glutamatergic synaptic transmission through lysophosphatidic acid (LPA)-mediated signaling pathways. Binds lysophosphatidic acid (LPA) and mediates its internalization into cells. Could act as receptor or a transporter of this lipid at the post-synaptic membrane. Modulates lysophosphatidic acid (LPA) activity in neuron axonal outgrowth during development by attenuating phospholipid-induced axon collapse.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Postsynaptic density membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I1U1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021469"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024451.1">
        <property type="protein sequence ID" value="ENSGAGP00000021469.1"/>
        <property type="gene ID" value="ENSGAGG00000015763.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03384">
        <property type="entry name" value="PAP2_wunen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000012">
        <property type="entry name" value="Phospholipid phosphatase-related protein type 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF13">
        <property type="entry name" value="PHOSPHOLIPID PHOSPHATASE-RELATED PROTEIN TYPE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="19">
        <location>
            <begin position="131"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="310"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="628"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="628"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00079928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="719" mass="78808" checksum="66597B01A70AA9FE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2D1</accession>
    <name>A0A452I2D1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger protein 219</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I2D1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021650"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024661.1">
        <property type="protein sequence ID" value="ENSGAGP00000021650.1"/>
        <property type="gene ID" value="ENSGAGG00000015885.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000075">
        <property type="entry name" value="Putative zinc finger protein 536"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001038">
        <property type="entry name" value="Zinc finger protein 217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001140">
        <property type="entry name" value="Zinc finger protein 219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000340">
        <property type="entry name" value="zinc finger protein 473 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051967">
        <property type="entry name" value="Krueppel_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="154"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="395"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="479"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="594"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="612"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="626"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="658" mass="71409" checksum="42EC6500C382A126" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2G1</accession>
    <name>A0A452I2G1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I2G1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021692"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024709.1">
        <property type="protein sequence ID" value="ENSGAGP00000021692.1"/>
        <property type="gene ID" value="ENSGAGG00000015225.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22936">
        <property type="entry name" value="shulin_C20orf194-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057478">
        <property type="entry name" value="DAAF9_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056414">
        <property type="entry name" value="DAAF9_CobW_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056498">
        <property type="entry name" value="DAAF9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040342">
        <property type="entry name" value="DNAAF9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058844">
        <property type="entry name" value="PB_DAAF9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058843">
        <property type="entry name" value="PH_DAAF9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33664:SF1">
        <property type="entry name" value="DYNEIN AXONEMAL ASSEMBLY FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33664">
        <property type="entry name" value="RCG26366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23319">
        <property type="entry name" value="CobW_C_DAAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25204">
        <property type="entry name" value="DAAF9_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23281">
        <property type="entry name" value="DAAF9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25203">
        <property type="entry name" value="PB_DAAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26246">
        <property type="entry name" value="PH_DAAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAAF9 N-terminal" evidence="3">
        <location>
            <begin position="25"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="DAAF9 pita-bread-like" evidence="5">
        <location>
            <begin position="224"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="DAAF9 PH" evidence="7">
        <location>
            <begin position="519"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="DAAF9" evidence="6">
        <location>
            <begin position="749"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="domain" description="DAAF9 CobW C-like" evidence="4">
        <location>
            <begin position="974"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1037"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1142"/>
            <end position="1169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1176" mass="133294" checksum="E89E81663C4952C3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2K6</accession>
    <name>A0A452I2K6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I2K6"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024757.1">
        <property type="protein sequence ID" value="ENSGAGP00000021732.1"/>
        <property type="gene ID" value="ENSGAGG00000015951.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050159">
        <property type="entry name" value="Kazal-type_SerProtInhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001239">
        <property type="entry name" value="Prot_inh_Kazal-m"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47499:SF1">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR KAZAL-TYPE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47499">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR KAZAL-TYPE 7 SPINK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00050">
        <property type="entry name" value="Kazal_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00290">
        <property type="entry name" value="KAZALINHBTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00282">
        <property type="entry name" value="KAZAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Kazal-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="64" mass="7657" checksum="DD9E4714AF38D31B" modified="2019-05-08" version="1">HRKRPNRLEYKKPPEACTREYFPICGTDGITYANKCEFCKAVYLCFEHYGECRLHDEAKLREDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I324</accession>
    <name>A0A452I324_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I324"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021884"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024928.1">
        <property type="protein sequence ID" value="ENSGAGP00000021884.1"/>
        <property type="gene ID" value="ENSGAGG00000016061.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005337">
        <property type="term" value="F:nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF17">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01130">
        <property type="entry name" value="DERENTRNSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="457"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="484" mass="53198" checksum="9C57CEFD3922D01E" modified="2019-05-08" version="1">MAVDLGDEDDRFQHNANSAYKPVNTSFQEEEPLIEEPVGNRYSLQKPKDHLNGAYVIFFILGIGSLLPWNFFITAKQYWIYKLQNCSNQQDPKGHGVSDLRDYFESYISIASTVPSVLCLIGNFVLVNRVSASIRILSSLAVMLAIFVVITVLVKMDTSTWTWHFFVITIICVVIVSSASTIFSSSIFGLTSCFPMKNSQALISGQAMGGTVSAVASVVDLAAAADVTDSALAYFLTADVFIIVCIGVYLILPKLEYSRYYMRSNKGSTYAAGVLPSSYLEGADESQGITNSPFPAKGTSVQNIPPLRPILMKTAVLGFCVFYVFFISIIIFPSISSSIESVNKDSGSLWTNKYFIPFTSFLLYNFADWCGRQITAWIQAPGPKSKLLPVLVLLRTLSLPLFMLCNYQPRIHIKMVIFNKDVYPAVFIALLGLSNGYLGTLAIIYGPKILPKELAEATGVVMTFYLTLGLALGSACSILLVHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8K9</accession>
    <name>A0A452I8K9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Perilipin 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the perilipin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I8K9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023865"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027182.1">
        <property type="protein sequence ID" value="ENSGAGP00000023865.1"/>
        <property type="gene ID" value="ENSGAGG00000017463.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010890">
        <property type="term" value="P:positive regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004279">
        <property type="entry name" value="Perilipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024">
        <property type="entry name" value="PERILIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024:SF11">
        <property type="entry name" value="PERILIPIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03036">
        <property type="entry name" value="Perilipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="8841" checksum="B1237B9049D0BDAB" modified="2019-05-08" version="1">QHAGEADTLLSASLPPEMNVLRRVASLPLVNSACDLAATAYASTKESHPYVRSICDMAEKGVTSITSAAVSSAQPVVTKLEPEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9L1</accession>
    <name>A0A452I9L1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-sensitive inward rectifier potassium channel 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Inward rectifier K(+) channel Kir6.2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Potassium channel, inwardly rectifying subfamily J member 11</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Inward rectifier potassium channel that forms the pore of ATP-sensitive potassium channels (KATP), regulating potassium permeability as a function of cytoplasmic ATP and ADP concentrations in many different cells. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. In pancreatic cells, it forms KATP channels with ABCC8/SUR1. Can form cardiac and smooth muscle-type KATP channels with ABCC9.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="22">Homotetramer; the homotetramer binds four ATP molecules (one ATP per subunit). Forms an heterooctamer with ABCC8/SUR1; one KCNJ11 homotetramer interacts with four ABCC8/SUR1 molecules. Interacts with ABCC9/SUR2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 24">Membrane</location>
            <topology evidence="1 24">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ11 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I9L1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024338"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027703.1">
        <property type="protein sequence ID" value="ENSGAGP00000024338.1"/>
        <property type="gene ID" value="ENSGAGG00000017784.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015272">
        <property type="term" value="F:ATP-activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000050">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000001">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003279">
        <property type="entry name" value="K_chnl_inward-rec_Kir6.2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF44">
        <property type="entry name" value="ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01332">
        <property type="entry name" value="KIR62CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17 24" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14 24" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 24" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 24" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 24 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 24" id="KW-0813">Transport</keyword>
    <keyword evidence="11 24" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="27">
        <location>
            <begin position="37"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="28">
        <location>
            <begin position="196"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="375"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="381"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="23">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00050001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR005465-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="400" mass="44974" checksum="F3B65FADCE7092DC" modified="2019-05-08" version="1">MLSRKGIIPEEYVLTRLAEDVQEHSRYRARERRARFVGKNGTCNVAHKNIREQGRFLQDVFTTLVDLKWPHTLIIFTMTFLCSWLLFGMTWWLIAFAHGDLDHSTQLQAGGSQGVGEEAEGFMPCVTSIHSFTSAFLFSIEVQVTIGFGGRMVTEECPAAILVLIVQNIVGLVINAIMLGCIFMKTAQAHRRAETLIFSKHAVIALRDGRLCFMLRVGDLRKSMIISATIRMQVVKKTTSLEGEVVPLNQIDIQMENPVGGSNIFLVSPLLIYHVIDKNSPLYDISPLHLHHHEDLEVIVILEGVVETTGITTQARTSYLADEILWGQRFVPIVAEEDGRYSVDYSKFGNTVKVPTPSCTARQLEEDRSIIDSIPLSPKSTSRKRSFRLKPKFTITDEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBI7</accession>
    <name>A0A452IBI7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Broad substrate specificity ATP-binding cassette transporter ABCG2</fullName>
            <ecNumber evidence="4">7.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">ATP-binding cassette sub-family G member 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Urate exporter</fullName>
        </alternativeName>
    </protein>
    <organism evidence="40 41">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="41" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="40" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>17beta-estradiol 17-O-(beta-D-glucuronate)(in) + ATP + H2O = 17beta-estradiol 17-O-(beta-D-glucuronate)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:82961"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:60129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>4-methylumbelliferone beta-D-glucuronate(in) + ATP + H2O = 4-methylumbelliferone beta-D-glucuronate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61372"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:144582"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:61373"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>4-methylumbelliferone sulfate(in) + ATP + H2O = 4-methylumbelliferone sulfate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61368"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:144581"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:61369"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>5,7-dimethyl-2-methylamino-4-(3-pyridylmethyl)-1,3-benzothiazol-6-yl beta-D-glucuronate(in) + ATP + H2O = 5,7-dimethyl-2-methylamino-4-(3-pyridylmethyl)-1,3-benzothiazol-6-yl beta-D-glucuronate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:144584"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:61385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>5,7-dimethyl-2-methylamino-4-(3-pyridylmethyl)-1,3-benzothiazol-6-yl sulfate(in) + ATP + H2O = 5,7-dimethyl-2-methylamino-4-(3-pyridylmethyl)-1,3-benzothiazol-6-yl sulfate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61376"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:144583"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:61377"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>dehydroepiandrosterone 3-sulfate(in) + ATP + H2O = dehydroepiandrosterone 3-sulfate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61364"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57905"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:61365"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>estrone 3-sulfate(in) + ATP + H2O = estrone 3-sulfate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61348"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60050"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:61349"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>indoxyl sulfate(in) + ATP + H2O = indoxyl sulfate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61332"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:144643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:61333"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>itaconate(in) + ATP + H2O = itaconate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:82291"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17240"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:82292"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>methotrexate(in) + ATP + H2O = methotrexate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61356"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:50681"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:61357"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>pheophorbide a(in) + ATP + H2O = pheophorbide a(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61360"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58687"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:61361"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>riboflavin(in) + ATP + H2O = riboflavin(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61352"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:61353"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>sphing-4-enine 1-phosphate(in) + ATP + H2O = sphing-4-enine 1-phosphate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38951"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60119"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:38952"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>urate(in) + ATP + H2O = urate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16461"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:16462"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="22">Homodimer; disulfide-linked. The minimal functional unit is a homodimer, but the major oligomeric form in plasma membrane is a homotetramer with possibility of higher order oligomerization up to homododecamers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452IBI7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025054"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028517.1">
        <property type="protein sequence ID" value="ENSGAGP00000025050.1"/>
        <property type="gene ID" value="ENSGAGG00000018321.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000028522.1">
        <property type="protein sequence ID" value="ENSGAGP00000025054.1"/>
        <property type="gene ID" value="ENSGAGG00000018321.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032217">
        <property type="term" value="F:riboflavin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000622">
        <property type="entry name" value="ATP-binding cassette sub-family G member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF92">
        <property type="entry name" value="BROAD SUBSTRATE SPECIFICITY ATP-BINDING CASSETTE TRANSPORTER ABCG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="16 38" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="41" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="9 38" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 38" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="38">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="38">
        <location>
            <begin position="477"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="38">
        <location>
            <begin position="503"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="38">
        <location>
            <begin position="537"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="38">
        <location>
            <begin position="634"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="39">
        <location>
            <begin position="36"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="37">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="37">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00031848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00046614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00047576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00047877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00048455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00048665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00049018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00049205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00049490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00052001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="39">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="658" mass="73335" checksum="7FA24CC8423017EF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBK2</accession>
    <name>A0A452IBK2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSGAGT00000028606.1">
        <property type="protein sequence ID" value="ENSGAGP00000025124.1"/>
        <property type="gene ID" value="ENSGAGG00000018379.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="117" mass="12967" checksum="D86D0CD6F5936EB1" modified="2019-05-08" version="1">MAPRQRPPSLLRRQPMPGSWDPSHQRIPFKGHLAAAKVPAGGCGPPRPTVSWAAPYLITCIWSGTSWSLGVRMVSLEQGKNYISQHSIGHYQLERKEGDLMQQRAVSGELXGICWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDF4</accession>
    <name>A0A452IDF4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000025843"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029398.1">
        <property type="protein sequence ID" value="ENSGAGP00000025843.1"/>
        <property type="gene ID" value="ENSGAGG00000018873.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF163">
        <property type="entry name" value="ZINC FINGER PROTEIN 589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="12"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="58" mass="6650" checksum="6DC2E0252BAF5F9C" modified="2019-05-08" version="1">MRIKHISSPILYNFHEVAVYFTKEEWALLDPAQRALCRDVMRIPHLLGSSXSLSPGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDQ5</accession>
    <name>A0A452IDQ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDQ5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025998"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029569.1">
        <property type="protein sequence ID" value="ENSGAGP00000025998.1"/>
        <property type="gene ID" value="ENSGAGG00000018988.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="47"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="318" mass="35690" checksum="F93267DF748497C2" modified="2019-05-08" version="1">MSATEAVRAGSHTAETEFILLGFGRGLWMEMFPFVMFLVIYIIIMLGNTILVLIFGADSQLHTSMYFLLMNLSLLELGNSSSIAPRAMVSFLSGSKAISYNGCATQFFFVTFFLTTEGFVLAAMAYDQYLAIRNPLRYPITMSKWVCIWLVVGSYICGCANSMVQTGFTFTLLFCGSNEMDHFFCDGPPLISLSCSDTFFNNIVMFTLCGLIIVSTALITLVSYVYIISTILRINSAEGRHKAFSTCTSHLVVVALFYVSTALMYAQLTRLAFLYPRKVVSIFYTLFAPMLNPFIYSLRNKDVKDALRRTMSKKCFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IE18</accession>
    <name>A0A452IE18_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESRP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IE18"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026133"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029720.1">
        <property type="protein sequence ID" value="ENSGAGP00000026133.1"/>
        <property type="gene ID" value="ENSGAGG00000019084.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12739">
        <property type="entry name" value="RRM2_ESRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12742">
        <property type="entry name" value="RRM3_ESRP1_ESRP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000041">
        <property type="entry name" value="Epithelial splicing regulatory protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000070">
        <property type="entry name" value="Epithelial splicing regulatory protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000056">
        <property type="entry name" value="epithelial splicing regulatory protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019317899" description="RRM domain-containing protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="225"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="326"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="667" mass="74101" checksum="B3CB9D2D1980C3A2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFW8</accession>
    <name>A0A452IFW8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger CCHC domain-containing protein 17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nucleolar protein of 40 kDa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Putative S1 RNA-binding domain protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts with PNN. Associates with the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IFW8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026791"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030448.1">
        <property type="protein sequence ID" value="ENSGAGP00000026791.1"/>
        <property type="gene ID" value="ENSGAGG00000019515.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043489">
        <property type="term" value="P:RNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05686">
        <property type="entry name" value="S1_pNO40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000154">
        <property type="entry name" value="nucleolar protein of 40 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047913">
        <property type="entry name" value="ZCCHC17_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15838">
        <property type="entry name" value="NUCLEOLAR PROTEIN OF 40 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15838:SF1">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="S1 motif" evidence="15">
        <location>
            <begin position="16"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="16">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="164"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="181"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="220"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="245" mass="27506" checksum="7DA44D6E262C06F5" modified="2019-05-08" version="1">MKSVRPETMETLPELYDVFQGEVAAVTEYGAFIKIPGCRKQGLVHRTHMSACRVDKPSEMVDVGDKVWVKLIGREMKDDKLKLSLSMKVVNQGTGKDLDPNNVALDQDERKKRSFRDYASQKITLEAVLNTVCKKCGCKGHFAKECFVQPGGTKYSLIPEEDETAEYEGDKKSSHAENSSKKKKKEKKKKKKHKSKKSSESNNSDSSDSDSDGAQPASKTAKRSGKASKPQKKKKKKKHKKKHKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGQ5</accession>
    <name>A0A452IGQ5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IGQ5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027057"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030747.1">
        <property type="protein sequence ID" value="ENSGAGP00000027057.1"/>
        <property type="gene ID" value="ENSGAGG00000019688.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013169">
        <property type="entry name" value="mRNA_splic_Cwf18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31551:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31551">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWF18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08315">
        <property type="entry name" value="cwf18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="169" mass="19151" checksum="794A321B5393EAB6" modified="2019-05-08" version="1">MAAPAAGSGVGRLQEEALRRRERLKALRSRTGAHKNKDNGEPENKQFREDDEEDGTKHKELKLRNYVPEDEDLKKRKVPQAKPASVEDKVKDQLEAAKPEPIIEEVDLANLAPRKPDWDLKRDVAKKLEKLEKRTQRAIAELIRERLRGQEEELASAVGSAKQEGSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGV4</accession>
    <name>A0A452IGV4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IGV4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027107"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030800.1">
        <property type="protein sequence ID" value="ENSGAGP00000027107.1"/>
        <property type="gene ID" value="ENSGAGG00000019715.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097120">
        <property type="term" value="P:receptor localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06723">
        <property type="entry name" value="PDZ1_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06724">
        <property type="entry name" value="PDZ2_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06795">
        <property type="entry name" value="PDZ3_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12031">
        <property type="entry name" value="SH3_DLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001402">
        <property type="entry name" value="Discs, large homolog 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000058">
        <property type="entry name" value="Disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000049">
        <property type="entry name" value="disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000002">
        <property type="entry name" value="Disks large homolog 4 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019583">
        <property type="entry name" value="DLG1-4_PDZ_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016313">
        <property type="entry name" value="DLG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019590">
        <property type="entry name" value="DLG1_PEST_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF5">
        <property type="entry name" value="DISKS LARGE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10608">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10600">
        <property type="entry name" value="PDZ_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001741">
        <property type="entry name" value="MAGUK_DLGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01277">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="177"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="272"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="421"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="536"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="9">
        <location>
            <begin position="682"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="619"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="872" mass="96085" checksum="F178183A580C7DAE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452II17</accession>
    <name>A0A452II17_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="38">24-hydroxycholesterol 7-alpha-hydroxylase</fullName>
            <ecNumber evidence="21">1.14.14.154</ecNumber>
            <ecNumber evidence="37">1.14.14.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="40">Cytochrome P450 39A1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Lanosterol 14-alpha demethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="39">Oxysterol 7-alpha-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="46 47">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="47" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="46" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="46" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="36">A cytochrome P450 monooxygenase involved in neural cholesterol clearance through bile acid synthesis. Catalyzes 7-alpha hydroxylation of (24S)-hydroxycholesterol, a neural oxysterol that is metabolized to bile acids in the liver. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>24,25-dihydrolanosterol + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:45960"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:28113"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:78904"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:45961"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>24,25-dihydrolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxy-24,25-dihydrolanosterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75079"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:28113"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:87057"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:75080"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>32-hydroxy-24,25-dihydrolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxo-24,25-dihydrolanosterol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75087"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:87057"/>
            <dbReference type="ChEBI" id="CHEBI:87060"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:75088"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>32-hydroxylanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxolanosterol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75107"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166681"/>
            <dbReference type="ChEBI" id="CHEBI:166806"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:75108"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>32-oxo-24,25-dihydrolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:75083"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:78904"/>
            <dbReference type="ChEBI" id="CHEBI:87060"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:75084"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>32-oxolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:75111"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166681"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:75112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>a 14alpha-formyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a Delta(14) steroid + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:68068"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138031"/>
            <dbReference type="ChEBI" id="CHEBI:176902"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:68069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>a 14alpha-hydroxymethyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-formyl steroid + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68064"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:176901"/>
            <dbReference type="ChEBI" id="CHEBI:176902"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:68065"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a 14alpha-methyl steroid + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = a Delta(14) steroid + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54028"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138029"/>
            <dbReference type="ChEBI" id="CHEBI:138031"/>
            <dbReference type="EC" id="1.14.14.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:54029"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>a 14alpha-methyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-hydroxymethyl steroid + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138029"/>
            <dbReference type="ChEBI" id="CHEBI:176901"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:68061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>lanosterol + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:25286"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:25287"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>lanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxylanosterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75103"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166806"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:75104"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 41 42">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; bile acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="20">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="35">Steroid metabolism; cholesterol degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 41 44">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.154" evidence="21"/>
    <dbReference type="EC" id="1.14.14.26" evidence="37"/>
    <dbReference type="AlphaFoldDB" id="A0A452II17"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027485"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031220.1">
        <property type="protein sequence ID" value="ENSGAGP00000027485.1"/>
        <property type="gene ID" value="ENSGAGG00000019977.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033782">
        <property type="term" value="F:24S-hydroxycholesterol 7-alpha-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008396">
        <property type="term" value="F:oxysterol 7-alpha-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008398">
        <property type="term" value="F:sterol 14-demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006699">
        <property type="term" value="P:bile acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20635">
        <property type="entry name" value="CYP39A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000060">
        <property type="entry name" value="Cytochrome P450 family 39 subfamily A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024204">
        <property type="entry name" value="Cyt_P450_CYP7A1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF2">
        <property type="entry name" value="24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000047">
        <property type="entry name" value="Cytochrome_CYPVIIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="10 41" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 41 42" id="KW-0349">Heme</keyword>
    <keyword evidence="14 41 42" id="KW-0408">Iron</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17 41" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 41 42" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0492">Microsome</keyword>
    <keyword evidence="15 44" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="13 44" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="47" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="45" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="18" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="45">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019517180" description="24-hydroxycholesterol 7-alpha-hydroxylase" evidence="45">
        <location>
            <begin position="24"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="binding site" evidence="43">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="43">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="42">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00037887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00038974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00041158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00047983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00048839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00048866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00049163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00049450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00049645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00056715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00066439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00071791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00077814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00078877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="PIRNR" id="PIRNR000047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="PIRSR" id="PIRSR000047-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="PIRSR" id="PIRSR000047-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="45">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="46">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="47">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="455" mass="51877" checksum="90B8AA0170E98394" modified="2019-05-08" version="1">MDITMAVLLALVLGLLMVKFALFQPRNPNSPPCISGWIPWLGAAFQFGKAPLEFIEQARVKYGPIFTVFALGNRFTFVTEEEGIDAFFTSKDIDFEQAVQQTVQRTTSVPAEIFYRNHSRLYSMMKGKMGTSSLYLFAGTLCQELHEHMDCLGTAGTENLSDLIRSVMYPATVNILFGKGICPTNKSDIKEFEEHFQKYDEGFEYGSQMPEYFMRNWSKSKKWLLKVFEKVVSDAERANLSDSDSKTLLQHLLDNLHGKHLAPNYALLLLWASQANAMPVAFWTLAFILSHPSVYKNIMEDLGSVFGKAGKENIEISEDDLKKLLLIKWCTLEAIRLRSPGAITKKVVNPIKIQTFTIPAGDMLMLSPYWVHRNPKYFPDPETFRPDRWKEANLEKNAFLDGFVAFGGGKHQCPGRWLAIMEVHMLVILFLYKYEFILLDPVPKEVNTCMFNPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIF0</accession>
    <name>A0A452IIF0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="9">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Has the potential to contribute to the immunosuppressive and toxic effects of FK506 and rapamycin. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Identified in a complex composed of RYR2, FKBP1B, PKA catalytic subunit, PRKAR2A, AKAP6, and the protein phosphatases PP2A and PP1. Interacts directly with RYR2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the FKBP-type PPIase family. FKBP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452IIF0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027619"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031376.1">
        <property type="protein sequence ID" value="ENSGAGP00000027619.1"/>
        <property type="gene ID" value="ENSGAGG00000020074.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010881">
        <property type="term" value="P:regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000035">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050689">
        <property type="entry name" value="FKBP-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10516:SF429">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="10">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="79" mass="8794" checksum="50428399CC5F1437" modified="2019-05-08" version="1">MLQNGKKFDSSRDRNKPFRFKIGRQEVIKGFEEGAAQMSLGQRAKLTCTPDMAYGATGHPGVIPPNATLIFDVELLRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJ09</accession>
    <name>A0A452IJ09_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">All trans-polyprenyl-diphosphate synthase PDSS1</fullName>
            <ecNumber evidence="15">2.5.1.91</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">All-trans-decaprenyl-diphosphate synthase subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Decaprenyl-diphosphate synthase subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Solanesyl-diphosphate synthase subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Trans-prenyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Heterotetrameric enzyme that catalyzes the condensation of farnesyl diphosphate (FPP), which acts as a primer, and isopentenyl diphosphate (IPP) to produce prenyl diphosphates of varying chain lengths and participates in the determination of the side chain of ubiquinone. Supplies nona and decaprenyl diphosphate, the precursors for the side chain of the isoprenoid quinones ubiquinone-9 (Q9)and ubiquinone-10 (Q10) respectively. The enzyme adds isopentenyl diphosphate molecules sequentially to farnesyl diphosphate with trans stereochemistry.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>6 isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = all-trans-nonaprenyl diphosphate + 6 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:55364"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58391"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:55365"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>7 isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = all-trans-decaprenyl diphosphate + 7 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27802"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60721"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.91"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:27803"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterotetramer composed of 2 PDSS1/DPS1 and 2 PDSS2/DLP1 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.91" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A452IJ09"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027852"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031638.1">
        <property type="protein sequence ID" value="ENSGAGP00000027852.1"/>
        <property type="gene ID" value="ENSGAGG00000020243.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032478">
        <property type="term" value="C:heterotetrameric polyprenyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097269">
        <property type="term" value="F:all-trans-decaprenyl-diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000011">
        <property type="entry name" value="Decaprenyl diphosphate synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF69">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00073240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00083689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00084036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="458" mass="50455" checksum="97FB619DEDDF7CC8" modified="2019-05-08" version="1">MGPGCPSPSPRIPAAAPPLPWPDLPAGERAAEQRFRVAAGAAASLGHAMAFWWRRCCFSRAPAFGSLRSLMEAAGPRLGSGSRAGWAVGGAEMHYFLGRSSVLSFPKIRSIFLRFHYTTTSLPCSCSKTVTDEKFKDPFKLGRKDLKNLYEDIKKELLVSTAELREMCDYYFDGKGKAFRPMIVVLMARACNIHHNNSGEVQASQRSVAIIAEMIHTASLVHDDVIDDANSRRGKMTANQIWGERKAVLAGDFILSAASVALARIGNTTIISVLSQVIEDLVRGEFLQLGSKENENERFAHYLEKTFKKTASLIANSCKAVSILGCPDPKVHEIAYQYGKNVGIAFQLIDDVLDFTSCADQLGKPAAADLKLGLATGPVLFACQQFPEMNAMIMRRFSLPGDIERAQQYVLQSDGVQQTTYLAQRYCHEAMKEISKLRPSPERDALIQLTETVLTRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJB9</accession>
    <name>A0A452IJB9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Diacylglycerol kinase</fullName>
            <shortName evidence="13">DAG kinase</shortName>
            <ecNumber evidence="13">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:40328"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:10273"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; glycerolipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A452IJB9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027959"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031758.1">
        <property type="protein sequence ID" value="ENSGAGP00000027959.1"/>
        <property type="gene ID" value="ENSGAGG00000020303.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00230"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20850">
        <property type="entry name" value="C1_DGKiota_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20896">
        <property type="entry name" value="C1_DGKiota_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000061">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000002">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047486">
        <property type="entry name" value="C1_DGKiota_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047487">
        <property type="entry name" value="C1_DGKiota_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056383">
        <property type="entry name" value="DGKI-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF92">
        <property type="entry name" value="DIACYLGLYCEROL KINASE IOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23578">
        <property type="entry name" value="DGKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="15">
        <location>
            <begin position="361"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="934"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="970"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="998"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="327"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1041" mass="116132" checksum="F94240E25FD8008A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJW7</accession>
    <name>A0A452IJW7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat ELP2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJW7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028114"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031946.1">
        <property type="protein sequence ID" value="ENSGAGP00000028114.1"/>
        <property type="gene ID" value="ENSGAGG00000020432.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000031949.1">
        <property type="protein sequence ID" value="ENSGAGP00000028117.1"/>
        <property type="gene ID" value="ENSGAGG00000020432.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000771">
        <property type="entry name" value="Elongator acetyltransferase complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000958">
        <property type="entry name" value="Elongator acetyltransferase complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001021">
        <property type="entry name" value="Elongator acetyltransferase complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037289">
        <property type="entry name" value="Elp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="15"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="164"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="347"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="573"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="628"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="795" mass="89066" checksum="80060B00AB816FDD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IL66</accession>
    <name>A0A452IL66_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IL66"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028766"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032681.1">
        <property type="protein sequence ID" value="ENSGAGP00000028766.1"/>
        <property type="gene ID" value="ENSGAGG00000020847.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="53" mass="6006" checksum="AE5A8A954DB3CAF2" modified="2019-05-08" version="1">QEVARHTVGPLNNQGTKGALEKDKVIVEKLNEFFKSVFTAEDLREIPTSEPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILV0</accession>
    <name>A0A452ILV0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HIG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILV0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028812"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032730.1">
        <property type="protein sequence ID" value="ENSGAGP00000028812.1"/>
        <property type="gene ID" value="ENSGAGG00000020875.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050355">
        <property type="entry name" value="RCF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297:SF10">
        <property type="entry name" value="HIG1 DOMAIN FAMILY MEMBER 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297">
        <property type="entry name" value="HYPOXIA-INDUCBILE GENE 1 HIG1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="7">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="97" mass="10781" checksum="2E21666C98DF5405" modified="2019-05-08" version="1">MSSDDRWSAEEDDSQVSKLTRKARDSPFVPVGMAGFVAVVGYGLYKLKHRGDQKMSVHLIHMRVAAQGFVVGAITIGVLFSMYKDYVKPQFKNGAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IM96</accession>
    <name>A0A452IM96_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm protein F</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IM96"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028961"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032897.1">
        <property type="protein sequence ID" value="ENSGAGP00000028961.1"/>
        <property type="gene ID" value="ENSGAGG00000020974.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01722">
        <property type="entry name" value="Sm_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034100">
        <property type="entry name" value="Sm_F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="26"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="119" mass="13320" checksum="29566259AD275A74" modified="2019-05-08" version="1">MCGPASNFCFHHCLVPGRITMSLPLNPKPFLNGLTGKLVMVKLKWGMEYKGYLVSVDGYMNMQLANTEEYIDGALSGHLGEAEVGDGVQGLPGVCRRLHEHAACKHRRIHRWCIVRTPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMM9</accession>
    <name>A0A452IMM9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cocaine- and amphetamine-regulated transcript protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CART family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IMM9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029106"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033062.1">
        <property type="protein sequence ID" value="ENSGAGP00000029106.1"/>
        <property type="gene ID" value="ENSGAGG00000021059.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008343">
        <property type="term" value="P:adult feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032099">
        <property type="term" value="P:negative regulation of appetite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22741">
        <property type="entry name" value="CART_CTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.40.30:FF:000001">
        <property type="entry name" value="Cocaine-and amphetamine-regulated transcript protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.30">
        <property type="entry name" value="CART, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009106">
        <property type="entry name" value="CART"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036722">
        <property type="entry name" value="CART_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16655">
        <property type="entry name" value="COCAINE AND AMPHETAMINE REGULATED TRANSCRIPT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16655:SF0">
        <property type="entry name" value="COCAINE- AND AMPHETAMINE-REGULATED TRANSCRIPT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06373">
        <property type="entry name" value="CART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64546">
        <property type="entry name" value="Satiety factor CART (cocaine and amphetamine regulated transcript)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="6" id="KW-0529">Neurotransmitter</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019559323" description="Cocaine- and amphetamine-regulated transcript protein" evidence="9">
        <location>
            <begin position="23"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="111" mass="12329" checksum="CE891B1F03A62679" modified="2019-05-08" version="1">MESARLLALLSATLVLLLGARGQEEPQLQPRALDLYPPMEDTSHEKELIEALQEVLEKLKSKRIPVYEKKFGQVPMCDAGEQCAVRKGARIGKLCDCPRGTSCNSFLLKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMT8</accession>
    <name>A0A452IMT8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Prostamide/prostaglandin F synthase</fullName>
            <ecNumber evidence="13">1.11.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Peroxiredoxin-like 2B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of prostaglandin-ethanolamide H(2) (prostamide H(2)) to prostamide F(2alpha) with NADPH as proton donor. Also able to reduce prostaglandin H(2) to prostaglandin F(2alpha).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>prostaglandin H2 + [thioredoxin]-dithiol = prostaglandin F2alpha + [thioredoxin]-disulfide</text>
            <dbReference type="Rhea" id="RHEA:28214"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57404"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="EC" id="1.11.1.20"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>prostamide F2alpha + [thioredoxin]-disulfide = prostamide H2 + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:26373"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:53081"/>
            <dbReference type="ChEBI" id="CHEBI:53082"/>
            <dbReference type="EC" id="1.11.1.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the peroxiredoxin-like PRXL2 family. Prostamide/prostaglandin F synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.20" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A452IMT8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029161"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033123.1">
        <property type="protein sequence ID" value="ENSGAGP00000029161.1"/>
        <property type="gene ID" value="ENSGAGG00000021099.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047017">
        <property type="term" value="F:prostaglandin F synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001516">
        <property type="term" value="P:prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02970">
        <property type="entry name" value="PRX_like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000243">
        <property type="entry name" value="Prostamide/prostaglandin F synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032801">
        <property type="entry name" value="PXL2A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28630:SF29">
        <property type="entry name" value="PROSTAMIDE_PROSTAGLANDIN F SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13911">
        <property type="entry name" value="AhpC-TSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0643">Prostaglandin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="201" mass="21944" checksum="9F80D3A8FD3FDB40" modified="2019-05-08" version="1">MAAVDLAKVGAHMLKHAVSGQVVELRALWRDQPCVVLFLRRFGCQVCRWIAKETSKLKDLLNSHNVRLIGVGPESVGLQEFIDGNYFKGELYLDETKQCYNDLGFKRYNALSIVPAALGKPVREVVAKANADGIQGNFSGDLLQSGGTIIVSQGGEKLLLHFIQDSPGDYVPLETIIKTLGISASVKEGVKPQCDEEACTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMZ8</accession>
    <name>A0A452IMZ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chemokine interleukin-8-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intercrine alpha (chemokine CxC) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IMZ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029239"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033210.1">
        <property type="protein sequence ID" value="ENSGAGP00000029239.1"/>
        <property type="gene ID" value="ENSGAGG00000021153.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00273">
        <property type="entry name" value="Chemokine_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000004">
        <property type="entry name" value="C-X-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001089">
        <property type="entry name" value="Chemokine_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033899">
        <property type="entry name" value="CXC_Chemokine_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF198">
        <property type="entry name" value="PLATELET BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00436">
        <property type="entry name" value="INTERLEUKIN8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00437">
        <property type="entry name" value="SMALLCYTKCXC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5028021094" description="Chemokine interleukin-8-like domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="6">
        <location>
            <begin position="30"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="94" mass="10539" checksum="E546DED477FAA538" modified="2019-05-08" version="1">MRAHTAALLVALLVICHSVYAAILETNGSYKSCRCTKQTSDFISPRRYESIEIVPFGGACRTTEIILKLKTGQKLCVNPKAPWMQKLLTKIRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452INT0</accession>
    <name>A0A452INT0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Resistance to inhibitors of cholinesterase protein 3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ric-3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452INT0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029554"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033561.1">
        <property type="protein sequence ID" value="ENSGAGP00000029554.1"/>
        <property type="gene ID" value="ENSGAGG00000021350.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034394">
        <property type="term" value="P:protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026160">
        <property type="entry name" value="Ric3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032763">
        <property type="entry name" value="RIC3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21723:SF3">
        <property type="entry name" value="PROTEIN RIC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21723">
        <property type="entry name" value="RESISTANCE TO INHIBITORS OF CHOLINESTERASE PROTEIN 3 RIC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15361">
        <property type="entry name" value="RIC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019020664" description="Resistance to inhibitors of cholinesterase protein 3 N-terminal domain-containing protein" evidence="10">
        <location>
            <begin position="30"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Resistance to inhibitors of cholinesterase protein 3 N-terminal" evidence="11">
        <location>
            <begin position="15"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="30"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="279"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF15361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="325" mass="35773" checksum="09C3CD70BEA6B7D2" modified="2019-05-08" version="1">MAYPAVRKVAMVSCLVLCVSLLLPRTFLSRGGRQEPGTAPPAPPEGKTSRFPPMMHHQTAPDGRSSGAHFPRSHLAEAVAKAKGGGGGGGSGRGLVGQIIPIYGFGILLYILYILYKLSSKGKTTTVERKSPSIPGNMNRKITDYELTQLQEKLRETEEAMEKLINRVGPNCDSRAQNVTTDQEKRLLQQLREITRVMKEGKLIDGISPEKAAEDAPYMEDWEECESHDMGQKKAQQISFSEQGGVLHHNHNFEKCYCCHYEEDDPAVIAENAGFHADSCSEAEDTAKDDHFMDSDNENSGLKSQKANDPDAVGTLRKRNTKGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IR24</accession>
    <name>A0A452IR24_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000030439"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034541.1">
        <property type="protein sequence ID" value="ENSGAGP00000030439.1"/>
        <property type="gene ID" value="ENSGAGG00000021920.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000042">
        <property type="entry name" value="C-myc promoter-binding protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.100.10.50:FF:000001">
        <property type="entry name" value="DENN domain containing 4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023341">
        <property type="entry name" value="MABP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF16">
        <property type="entry name" value="C-MYC PROMOTER-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51498">
        <property type="entry name" value="MABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MABP" evidence="6">
        <location>
            <begin position="41"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="UDENN" evidence="5">
        <location>
            <begin position="191"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="809"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="918"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1188"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1239"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1384"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1614"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="926"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="947"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1191"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1279"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1291"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1392"/>
            <end position="1404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1896" mass="212637" checksum="CFE0CAA92A204564" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IR65</accession>
    <name>A0A452IR65_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">ATP-dependent DNA helicase Q4</fullName>
            <ecNumber evidence="17">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">DNA 3'-5' helicase RecQ4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">DNA helicase, RecQ-like type 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">RecQ protein-like 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="17"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030469"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034574.1">
        <property type="protein sequence ID" value="ENSGAGP00000030469.1"/>
        <property type="gene ID" value="ENSGAGG00000021941.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18018">
        <property type="entry name" value="DEXHc_RecQ4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22289">
        <property type="entry name" value="RecQL4_SLD2_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000772">
        <property type="entry name" value="ATP-dependent DNA helicase Q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1460:FF:000001">
        <property type="entry name" value="DNA replication regulator Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001084">
        <property type="entry name" value="RecQ like helicase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021110">
        <property type="entry name" value="DNA_rep_checkpnt_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF108">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11719">
        <property type="entry name" value="Drc1-Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="23" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="25">
        <location>
            <begin position="582"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="26">
        <location>
            <begin position="714"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="27">
        <location>
            <begin position="915"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="131"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="326"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="372"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="328"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="485"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00074290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00076756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00084018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1470" mass="163018" checksum="AC9E1CEBC33634AD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRH0</accession>
    <name>A0A452IRH0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-alpha family. G(12) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IRH0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030586"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034707.1">
        <property type="protein sequence ID" value="ENSGAGP00000030586.1"/>
        <property type="gene ID" value="ENSGAGG00000022025.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031752">
        <property type="term" value="F:D5 dopamine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000004">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000754">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000469">
        <property type="entry name" value="Gprotein_alpha_12/13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF130">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT ALPHA-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00440">
        <property type="entry name" value="GPROTEINA12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="50"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="185"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="210"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="279"/>
            <end position="282"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="364" mass="42664" checksum="7AA793F4AEF399ED" modified="2019-05-08" version="1">MAGVVRTLSRCLLPAEREARRRSREIDAMLARERRAVRRLVKILLLGAGESGKSTFLKQMRIIHGREFDQKALLEFRAIIYENVVKGCKVLVDARDKLRIPWQYTENEKHGMFLMAFENKAGVSIEPATFQLYVPALSGLWRDSGIKEAFSRRSEYQLGESVKYFLDNLDRIGQLNYFPSKQDILLARKATKGIVEHDFIIKKIPFKMVDVGGQRSQRQKWFQCFDGITSILFMVSSSEYDQVLMEDRRTNRLVESMNIFETIVNNKLFFNVSIILFLNKMDLLVEKVKTVSIKKYFSDFKGDPHRLDDVQRYLVQCFDRKRRNRSKPLFHHFTTAIDTENIRFVFHAVKDTILQENLKDIMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IT62</accession>
    <name>A0A452IT62_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IT62"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031136"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035306.1">
        <property type="protein sequence ID" value="ENSGAGP00000031136.1"/>
        <property type="gene ID" value="ENSGAGG00000022368.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043549">
        <property type="entry name" value="C2C4C/C2C4D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46291">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46291:SF8">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="267"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="382" mass="43076" checksum="16EAF9D356C24EB0" modified="2019-05-08" version="1">KWKTNTNITSHLSLHNLETTGLNSGSLGARKAMVASPNILTPDRIPEFCIPSRLISSHSARDPSSHIQSYAETNLYPHCEPDHNPSTQLPHILSTEIVNNFISVEESTNSDPQSQAALSLLHFPKVQTSYGFCTLLESPHTRRKESIFHSDPCSCSMSCLLLLPSSRICYKGVDSSIISSTATRLPSWHPHLPRQDTYDYDTASSYSLFTIQSREGLLCRALKAENKSSMLRENSLSTDEDSSTDNSSNPNSTQKRLRNARTVFLDRRGILRLSSDYCPESRRLRLCLISAEGLYDVFMEPKRINCCITFCLVPGKVQKQRSTIIKRSRNPIFNEDFVFDGISKDDLHRRSIKIKAVNKASTMKRDYILGKIELYLLSMVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITH1</accession>
    <name>A0A452ITH1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ITH1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031409"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035625.1">
        <property type="protein sequence ID" value="ENSGAGP00000031409.1"/>
        <property type="gene ID" value="ENSGAGG00000022569.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040386">
        <property type="entry name" value="P66"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032346">
        <property type="entry name" value="P66_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13455:SF3">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR P66-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13455">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR P66-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16563">
        <property type="entry name" value="P66_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="424"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="184"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="508"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="184"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="617" mass="66939" checksum="3E16B85DB3503608" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWV7</accession>
    <name>A0A452IWV7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IWV7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032400"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036732.1">
        <property type="protein sequence ID" value="ENSGAGP00000032400.1"/>
        <property type="gene ID" value="ENSGAGG00000023180.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097527">
        <property type="term" value="P:necroptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21037">
        <property type="entry name" value="MLKL_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000437">
        <property type="entry name" value="Mixed lineage kinase domain-like pseudokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059179">
        <property type="entry name" value="MLKL-like_MCAfunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054000">
        <property type="entry name" value="MLKL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF298">
        <property type="entry name" value="MIXED LINEAGE KINASE DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22215">
        <property type="entry name" value="MLKL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="210"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="492" mass="56490" checksum="1BEB76D202FAE7C4" modified="2019-05-08" version="1">MEVVEKVLSVAHIIYAQCEQVKCCKHQCRRLVYRVHILLRPVEVLRAQPPKQISSQVEETLQQLLETLEKAQELVKKYSQTNWLQKFLKASDVVEVFARVNERLGDAAQGLSLLLQAEHKQSFLETFQRDTCSREDSQDTKDDKALWEELLTRSAETKDAVDNVHKDVRHVGTKVEDVGSRVEEVRQMMQKLQDAVMKKDNAVPREEITEIRMEDLTKTSWTLLMESKSHTLYKGEFYKYPVAIKVFKNPVITSTEKVREIFRKEIETMKKFESPNILRMYGICIDERGSSPCFSIVMEYCEKGTLRDVLTRKPQLPWETRIRMALDAARGLYRLHQTGEKSKLHGCINSSKFLVAKGYCVKLSGFELSKTESSIKRSCRKKLTDVPASAYISPLGLASLSHKYDVPSEIYSFGIVLWEIATSKIPFEGCSSQEIYQKVYEQRYQAPVGEDCPSHLRDVINQCRAFEPLQRPSAEEIVDSLIAIYGKINTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IX51</accession>
    <name>A0A452IX51_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">VLIG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Very large inducible GTPase (VLIG) family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000032514"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036861.1">
        <property type="protein sequence ID" value="ENSGAGP00000032514.1"/>
        <property type="gene ID" value="ENSGAGG00000023257.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030383">
        <property type="entry name" value="G_VLIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058641">
        <property type="entry name" value="GVIN1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057365">
        <property type="entry name" value="URGCP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22796:SF6">
        <property type="entry name" value="INTERFERON-INDUCED VERY LARGE GTPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22796">
        <property type="entry name" value="URG4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25496">
        <property type="entry name" value="URGCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25974">
        <property type="entry name" value="URGCP_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25683">
        <property type="entry name" value="URGCP_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51717">
        <property type="entry name" value="G_VLIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VLIG-type G" evidence="9">
        <location>
            <begin position="1499"/>
            <end position="1740"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="88"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1838"/>
            <end position="1883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2443" mass="279687" checksum="F411442EA3021E86" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IXI9</accession>
    <name>A0A452IXI9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IXI9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032767"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037131.1">
        <property type="protein sequence ID" value="ENSGAGP00000032767.1"/>
        <property type="gene ID" value="ENSGAGG00000023412.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000001">
        <property type="entry name" value="GATA transcription factor (GATAd)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000032">
        <property type="entry name" value="Transcription factor GATA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008013">
        <property type="entry name" value="GATA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016375">
        <property type="entry name" value="TF_GATA_4/5/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF289">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05349">
        <property type="entry name" value="GATA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003028">
        <property type="entry name" value="TF_GATA_4/5/6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="14">
        <location>
            <begin position="184"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="14">
        <location>
            <begin position="238"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="GATA-type 1" evidence="11">
        <location>
            <begin position="190"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="GATA-type 2" evidence="11">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="57"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="286"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR003028-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="398" mass="42980" checksum="500DE8D2B4F8D956" modified="2019-05-08" version="1">MYQSLALAPNHGQSAYSHDSSNFLHSSASSPVYVPTTRVPSMLQTLPYLQGCETAHQSHPLGNHPGWAQTGAEASAFNPSSPHPSSGFPYSHSPPVSNNAGREGAYQSPLLLGSGAREQYSNALVRSVNGSYSSPYPAYVSPEMPPSWTAGHFESSVLHSLQTRQAALPGRRSTFVSEYLEEFPGDGRECVNCGAMSTPLWRKDGTGHYLCNACGLYHKMNGINRPLIKPQKRLSSSRRAGLCCTNCHTTNTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKESIQTRKRKPKNITKGKTSTGSTTSATNSPSSITNSDSTITLKTEPNVASQYPGQTIVSVSQAQSQSDDTLASGHSEFKFEPEDYAFTSATMTQQSGLSIPLRQDSWCALALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IXN7</accession>
    <name>A0A452IXN7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Arachidonate--CoA ligase</fullName>
            <ecNumber evidence="12">6.2.1.15</ecNumber>
            <ecNumber evidence="13">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19713"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19714"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(E)-hexadec-2-enoate + ATP + CoA = (2E)-hexadecenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36139"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:72745"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + ATP + CoA = 12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52112"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:90718"/>
            <dbReference type="ChEBI" id="CHEBI:136408"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:52113"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + ATP + CoA = 15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52116"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:78832"/>
            <dbReference type="ChEBI" id="CHEBI:136409"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:52117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = 5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65341"/>
            <dbReference type="ChEBI" id="CHEBI:136407"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:52109"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>hexadecanoate + ATP + CoA = hexadecanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30751"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:30752"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.15" evidence="12"/>
    <dbReference type="EC" id="6.2.1.3" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A452IXN7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032822"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037200.1">
        <property type="protein sequence ID" value="ENSGAGP00000032822.1"/>
        <property type="gene ID" value="ENSGAGG00000023197.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047676">
        <property type="term" value="F:arachidonate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035338">
        <property type="term" value="P:long-chain fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010747">
        <property type="term" value="P:positive regulation of long-chain fatty acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF107">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="16">
        <location>
            <begin position="147"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="711" mass="78907" checksum="A2CCEDD0F6FECF1D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IXX8</accession>
    <name>A0A452IXX8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IXX8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037319.1">
        <property type="protein sequence ID" value="ENSGAGP00000032928.1"/>
        <property type="gene ID" value="ENSGAGG00000023515.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="60"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="166" mass="18901" checksum="DF39F7F6E5B22951" modified="2019-05-08" version="1">MGKRLEQQPMYPQYTYYYPHYLQTKSYAPAPHPMAPPSPSTNSSNNSSNNSSGEQLSKTNLYIRGLPPGTTDQDLIKLCQPYGKIVSTKAILDKNTNQCKGMCTEKELVLFSLTKSRLRGDMIALYKYIRGINIREGEELFKLNTNVDTRTNGYKLAIRKLRLEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IY54</accession>
    <name>A0A452IY54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ran-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with CHC1 in a Ran-stimulated manner. Interacts with XPO1. Interacts (via its C-terminal R domain) with SMAD2 (dephosphorylated form via its MH1 and MH2 domains); the interaction results in the nuclear export of SMAD2 and termination of the TGF-beta signaling. Interacts (via its C-terminal R domain) with SMAD3 (dephosphorylated form via its MH1 domain); the interaction results in the nuclear export of SMAD3 and termination of the TGF-beta signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IY54"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032981"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037383.1">
        <property type="protein sequence ID" value="ENSGAGP00000032981.1"/>
        <property type="gene ID" value="ENSGAGG00000023549.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13180">
        <property type="entry name" value="RanBD_RanBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000106">
        <property type="entry name" value="ran-binding protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF91">
        <property type="entry name" value="RAN-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RanBD1" evidence="13">
        <location>
            <begin position="356"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="76"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="303"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="490"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="537" mass="57938" checksum="DF048DA1635A0FA1" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZX9</accession>
    <name>A0A452IZX9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the POU transcription factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZX9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033663"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038129.1">
        <property type="protein sequence ID" value="ENSGAGP00000033663.1"/>
        <property type="gene ID" value="ENSGAGG00000023961.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000326">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF125">
        <property type="entry name" value="POU DOMAIN, CLASS 3, TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="11">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="55"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="57"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="169" mass="19169" checksum="010E35079236BAEC" modified="2019-05-08" version="1">MGMFSQTTICRFEALQLSFKNMCKLKPLLQRWLNEVENSDSLQELCNAEQVLAQARKRKRRTSIENNVKGTLESFFRNCVKPSPQQISQIAEDLNLDKDVVRVWFCNRRQKGKRLESWALASPWQMPACGSALLPGPVDTPLTVPALSQSVGIFQGEYVSLNNARCMCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J051</accession>
    <name>A0A452J051_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="9">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452J051"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033765"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038246.1">
        <property type="protein sequence ID" value="ENSGAGP00000033765.1"/>
        <property type="gene ID" value="ENSGAGG00000024034.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000003">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF0">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0274">FAD</keyword>
    <keyword evidence="3 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="132" mass="15331" checksum="C67C4DA24D8BCB63" modified="2019-05-08" version="1">VCSISVGLAKSFLQGNQTYFCSPNRKKELGRNTWAFLHTMAAYYPDHPSNTQQHEMAQFMDLLSKFYPCVKCAKNLRKSLSIKKPDTSNRKKLSQWMCLLHNDVNKMLGKPEFDCSQMDERWREGWKDGSCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J101</accession>
    <name>A0A452J101_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pleiotrophin</fullName>
            <shortName evidence="8">PTN</shortName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Secreted growth factor that mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Regulates many processes like cell proliferation, cell survival, cell growth, cell differentiation and cell migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the pleiotrophin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J101"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034039"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038543.1">
        <property type="protein sequence ID" value="ENSGAGP00000034039.1"/>
        <property type="gene ID" value="ENSGAGG00000024186.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.90.10:FF:000001">
        <property type="entry name" value="Pleiotrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.90.10">
        <property type="entry name" value="Heparin-binding Growth Factor, Midkine, Chain A- C-terminal Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000762">
        <property type="entry name" value="Midkine_heparin-bd_GF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020090">
        <property type="entry name" value="PTN/MK_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038130">
        <property type="entry name" value="PTN/MK_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020091">
        <property type="entry name" value="PTN/MK_diS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13850:SF1">
        <property type="entry name" value="PLEIOTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13850">
        <property type="entry name" value="PLEIOTROPHIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01091">
        <property type="entry name" value="PTN_MK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00269">
        <property type="entry name" value="PTNMIDKINE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00193">
        <property type="entry name" value="PTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57288">
        <property type="entry name" value="Midkine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="4 8" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 8" id="KW-0497">Mitogen</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Pleiotrophin/Midkine C-terminal" evidence="11">
        <location>
            <begin position="160"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="42"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="230" mass="25303" checksum="D173639A1953B647" modified="2019-05-08" version="1">EELKSCTSPGPTPSPIPLPSSSPGARDCPWSDLLLISFPSRSHECKVPSRALPPSSPARSLRASESKRGEASSLPRAAQSWRAHPSRRGEARSPQIQPERDSSARRSQAAKPTAQGSWADTSTMQQQQQQRRMFTAALLAFIFILAAMGTTEAGKKEKPAECKYQFQAWGECDLNTALKTRTGNLKRALHNADCQKIVTISKPCGKLTKPKPQEPKKKKKEGKKQEKMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J3B7</accession>
    <name>A0A452J3B7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small integral membrane protein 33</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J3B7"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039537.1">
        <property type="protein sequence ID" value="ENSGAGP00000034915.1"/>
        <property type="gene ID" value="ENSGAGG00000024824.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039954">
        <property type="entry name" value="DUF5527"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038803">
        <property type="entry name" value="SMIM33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37873">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37873:SF1">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17665">
        <property type="entry name" value="DUF5527"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="15730" checksum="695891501F1898B1" modified="2019-05-08" version="1">MNISTPSSQPRQHEPLDLAGFTTGSVVRGVTKKADALPMITVIVVIFVLLAVVIIVLVRYGTQLCTLQITLYHEPMPQDLENGVHLTDWKKLGSKRKNSMQPAQNYQGEPGSNDTAVLSVQCSCKHQLPCGNMEPNVIEITYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GF01</accession>
    <name>A0A452GF01_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-H</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Associates primarily with CDK7 and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GF01"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000112"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000129.1">
        <property type="protein sequence ID" value="ENSGAGP00000000112.1"/>
        <property type="gene ID" value="ENSGAGG00000000095.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20524">
        <property type="entry name" value="CYCLIN_CCNH_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20525">
        <property type="entry name" value="CYCLIN_CCNH_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000029">
        <property type="entry name" value="Cyclin h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000044">
        <property type="entry name" value="cyclin-H isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031658">
        <property type="entry name" value="Cyclin_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027081">
        <property type="entry name" value="CyclinH/Ccl1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00569">
        <property type="entry name" value="ccl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16899">
        <property type="entry name" value="Cyclin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 12" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="13">
        <location>
            <begin position="62"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="323" mass="37462" checksum="C6A7357DED97F60C" modified="2019-05-08" version="1">MYHSSTQRRHWTFRSEEELTRCRADANRKFCCKAVACGKARQSDSLLLEPREELAICKYYERKLLDFCSVFKPAMPRSVVGTATMYFKRFYLNNSVMEYHPRIIMLTCAFLACKVDEFNVSSAQFVGNLRENPPGQEKALEQILEYELLLIQQLNFHLIVHNPYRPFEGFLIDLKTRYPLLENPEVLRKTADDFLTRVALTDAYLLFTPSQIALTAILSSASRAGINMESYLSECLALKENRTSLSQLLDGMKCMKNLIKKYEPPRPEEVATLKQKLEKCHSSELALNVNVKKRKGYEDDEYITKKSKMDEEEWTDDDLVDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GF62</accession>
    <name>A0A452GF62_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">General transcription factor IIF subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Transcription initiation factor IIF subunit beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TFIIF beta subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GF62"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000189"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000218.1">
        <property type="protein sequence ID" value="ENSGAGP00000000189.1"/>
        <property type="gene ID" value="ENSGAGG00000000165.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07980">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000035">
        <property type="entry name" value="General transcription factor IIF subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003196">
        <property type="entry name" value="TFIIF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040450">
        <property type="entry name" value="TFIIF_beta_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040504">
        <property type="entry name" value="TFIIF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02270">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17683">
        <property type="entry name" value="TFIIF_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015849">
        <property type="entry name" value="TFIIF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIF beta subunit N-terminal" evidence="12">
        <location>
            <begin position="16"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIF beta subunit HTH" evidence="11">
        <location>
            <begin position="189"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="262" mass="29794" checksum="EEBA0D749A7D1614" modified="2019-05-08" version="1">MSEKGELDLTGAKQNTGMWLVKVPKYLSQQWTKASGRGEVGKLRIAKNQGRTEVSFTLNEDLASINDIGGKPASVSAPREHPFLLQSVGGQTLTVFTESSGESQPEEKSENSNADKLSLEGIVVQRAECRPAASENYMKLKRLQIEESSKPVRLSQQLDKAVTTNYKPVANHQYNIEYEKKKKEDGKRARADKQQVLDMLFSAFEKHQYYNIKDLVDITKQPVIYLKEILREIGIYNVKGTHKNTWELKPEYRHYQGEEKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGK4</accession>
    <name>A0A452GGK4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GGK4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000699"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000788.1">
        <property type="protein sequence ID" value="ENSGAGP00000000699.1"/>
        <property type="gene ID" value="ENSGAGG00000000590.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026205">
        <property type="entry name" value="PIBF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18950:SF0">
        <property type="entry name" value="PROGESTERONE IMMUNOMODULATORY BINDING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18950">
        <property type="entry name" value="PROGESTERONE-INDUCED BLOCKING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="198"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="474"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="584"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="688" mass="82089" checksum="AC36F847703EC466" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHB0</accession>
    <name>A0A452GHB0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GHB0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000953"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001078.1">
        <property type="protein sequence ID" value="ENSGAGP00000000953.1"/>
        <property type="gene ID" value="ENSGAGG00000000780.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034113">
        <property type="term" value="P:heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150079">
        <property type="term" value="P:negative regulation of neuroinflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000304">
        <property type="entry name" value="Nectin cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047164">
        <property type="entry name" value="OX2G-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46841">
        <property type="entry name" value="OX-2 MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46841:SF3">
        <property type="entry name" value="OX-2 MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019182704" description="Ig-like domain-containing protein" evidence="16">
        <location>
            <begin position="21"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="18"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="261" mass="29506" checksum="29D113E2F8E41E53" modified="2019-05-08" version="1">MTYQGLILSVVFIMLCRTEAELVTQKKVVEVGGNVTLSCLLMGSYEILQVTWQKKNTGVNENLATYSLSKGINIAETYRGRLNFTRLSLNDTAITLWRARIQDDGCYTCLFNTFPDGAIPQHTCLTVYEQLRGSLHYVVSEGNLTATCSATAWPRPVISWIVPKAESEQTEEVITHPNGTVSVISKLYVNSSTSLAGQLLICRVQHMERDRDYSVKVKDGRWFSVPWFLATVVLLIVFVVLVLTVVCWRRRRKKQSEFRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHE7</accession>
    <name>A0A452GHE7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GHE7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001043"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001175.1">
        <property type="protein sequence ID" value="ENSGAGP00000001043.1"/>
        <property type="gene ID" value="ENSGAGG00000000841.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003973">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000666">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001228">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000340">
        <property type="entry name" value="zinc finger protein 473 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000771">
        <property type="entry name" value="zinc finger protein 648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF114">
        <property type="entry name" value="KLUMPFUSS, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="288"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="306"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="379" mass="43029" checksum="6956E46116B37AF7" modified="2019-05-08" version="1">INSISPRMEIPPVSPSLPFSIEHGAIQIPSQREFSMCVSGEGSTPKSHERRSRPHLKPPAEGQSEPRKTVYTCRVCREEFKVQRDLIDHHWTVHKRHKLYHCSECGESFRRRKAFKAHGKIHRKERAHPCSECGKIFNRASHLIAHQRIHTRERPYHCTECGKRFFQISVLNIHKRIHSGERPFACAECGRCFMYSSTLNRHQRIHSEKQLHCCNQCGKGFKFTSSLTRHLRIHSGERPFLSHECGKRFNLQEHLIRHRTIHTGERPHQRPYPCTQCGKRFAHSLSLAKHQRTHGKPLPALTSPFSPQPPPSPHPYPASCHPVPPPPPSAPRPHSSLSAQDGPADCSGQEALGEELISRATGGREALQREGEELAAGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIS2</accession>
    <name>A0A452GIS2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GIS2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001502"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001713.1">
        <property type="protein sequence ID" value="ENSGAGP00000001502.1"/>
        <property type="gene ID" value="ENSGAGG00000001210.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="305"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="31"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="499" mass="54696" checksum="4150BA2355741CA4" modified="2019-05-08" version="1">MWTFAVYDNYSIPMLLGEDLANQVKRAKRVGMVTRSQTRQASRPIPVPEPSTEAPSVLPETQTEVVDPDSMPTTETATASPVPGPELEQQPAPASATTSSNSTPEGASEPELAEAKDSHTQKAQPEPEIPSGAPAESGSPATETTPSPTSLPEGPSPSPQSEEELVTPASREQFQTEQEADDSLQKAWAAARSTPPPLSSSNRSRFVIDQGLLYKEILSGGHREEWQPQKQLVVPTKYRGKLLSLAHDHPSGHAGVNRTKDRLGKSFHWEGMGKDVAKYVRSCEVCQRVGKPQDQVKAPLQPLPIIEVPFQRVAVDILGPFPKKTPRGKQYVLTLVDFATRWPEAVALGNTRANTVCLALTDIFARVGWPSDILTDSGSNFLAGTMEKLWETHGVNHLVATPYHHQTNGLVERFNGTLGAMIRKFINEFSNNWDLVLQQLLFAYRAVPHPSLGFSPFELVYGHEVKGPLQLVKQQWEGFTPSPGTNILDFVSNLQSTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJB6</accession>
    <name>A0A452GJB6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Chromodomain-helicase-DNA-binding protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination. Involved in neurogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000001768"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002010.1">
        <property type="protein sequence ID" value="ENSGAGP00000001768.1"/>
        <property type="gene ID" value="ENSGAGG00000001416.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18667">
        <property type="entry name" value="CD1_tandem_CHD3-4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18662">
        <property type="entry name" value="CD2_tandem_CHD3-4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18056">
        <property type="entry name" value="DEXHc_CHD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15531">
        <property type="entry name" value="PHD1_CHD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15532">
        <property type="entry name" value="PHD2_CHD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000037">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000003">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000011">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000011">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000015">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012957">
        <property type="entry name" value="CHD_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009462">
        <property type="entry name" value="CHD_II_SANT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012958">
        <property type="entry name" value="CHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009463">
        <property type="entry name" value="DUF1087"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF22">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08074">
        <property type="entry name" value="CHDCT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06461">
        <property type="entry name" value="CHDII_SANT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08073">
        <property type="entry name" value="CHDNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06465">
        <property type="entry name" value="DUF1087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01146">
        <property type="entry name" value="DUF1086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01147">
        <property type="entry name" value="DUF1087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="20" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="19" id="KW-0804">Transcription</keyword>
    <keyword evidence="17" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 25" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="28">
        <location>
            <begin position="425"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="28">
        <location>
            <begin position="504"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="27">
        <location>
            <begin position="584"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="27">
        <location>
            <begin position="677"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="29">
        <location>
            <begin position="793"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="30">
        <location>
            <begin position="1109"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="26"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="303"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="380"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="571"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="633"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1398"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1583"/>
            <end position="1727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1953"/>
            <end position="1975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="26">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="26">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="26">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="380"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="26">
        <location>
            <begin position="394"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="26">
        <location>
            <begin position="1589"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="1616"/>
            <end position="1639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="1673"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="1716"/>
            <end position="1727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00074666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00093442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1975" mass="225312" checksum="2D672FA360121E9B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKM2</accession>
    <name>A0A452GKM2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BET1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Golgi vesicular membrane-trafficking protein p18</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Required for vesicular transport from the ER to the Golgi complex. Functions as a SNARE involved in the docking process of ER-derived vesicles with the cis-Golgi membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with SNARE complex members GOSR2, SEC22B and STX5. Interacts with LMAN1/ERGIC53. Interacts with STX17.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endomembrane system</location>
            <topology evidence="14">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">cis-Golgi network membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the BET1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GKM2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002198"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002509.1">
        <property type="protein sequence ID" value="ENSGAGP00000002198.1"/>
        <property type="gene ID" value="ENSGAGG00000001782.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000026">
        <property type="entry name" value="BET1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="20">
        <location>
            <begin position="34"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="126" mass="13976" checksum="6DAA521EE15243A0" modified="2019-05-08" version="1">MNMLLSGAGTITCTARCSEGAATGNYGYTNSGYSVHEEENERLTESLRTKVSAIKSLSIEIGTEVKNQNKMLSDMDSDFDSTGGFLDATIGRLKTLSRGSQTKLLCYMMLFALFVFFVIYWIIKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GM36</accession>
    <name>A0A452GM36_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GM36"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003145.1">
        <property type="protein sequence ID" value="ENSGAGP00000002749.1"/>
        <property type="gene ID" value="ENSGAGG00000002199.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="81" mass="9218" checksum="A44C1ADD23B9F884" modified="2019-05-08" version="1">SGFLCMVSALPFMRSRHSCWELLESSGKQCLLGRLLYPSPFYPGWLREYKTRSSPTTLQFLLTSEGSVSLDCTHCPKCFPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMG8</accession>
    <name>A0A452GMG8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMG8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003014"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003454.1">
        <property type="protein sequence ID" value="ENSGAGP00000003014.1"/>
        <property type="gene ID" value="ENSGAGG00000002410.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001747">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003095">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000759">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000506">
        <property type="entry name" value="Zinc finger protein 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000176">
        <property type="entry name" value="zinc finger protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001450">
        <property type="entry name" value="zinc finger protein 774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF158">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="8"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="358" mass="40984" checksum="EFA516B6782594EF" modified="2019-05-08" version="1">MVSENEEEKPHQEGAELREAHGMSSGRSKENVSRSCALPEKPKARETQGRPEGNFSSLSVLKASGRINLDETCYTCTECGKSFNRSSHLITHRRIHTGETPYTCAECGRSFNQSSTLNTHCRIHTGETPYTCTECGKSFSQSSTLNTHHRIHTGEKPYTCIECGKNFTQCSTLNTHRRIHTGETPYTCSECGRSFNRSSHLIGHQRIHAREMPYTCSECGKSFSQRAHLITHQKVHTGETLYTCAECGKSFSQRSHLIRHRRIHTGETPYTCAECGKSFNRRSNLIRHQRMHTRETLYTCSECGKSFNRSSTLNTHRRIHTREMPYTCSECGQSFNQRSRLIRHQKMHRRENCNKSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMI3</accession>
    <name>A0A452GMI3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Serine/threonine-protein kinase MARK1</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
            <ecNumber evidence="5">2.7.11.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">MAP/microtubule affinity-regulating kinase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Serine/threonine-protein kinase. Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22 35">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[tau protein] + ATP = O-phospho-L-seryl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12801"/>
            <dbReference type="Rhea" id="RHEA-COMP:13701"/>
            <dbReference type="Rhea" id="RHEA-COMP:13702"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 35">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-threonyl-[tau protein] + ATP = O-phospho-L-threonyl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53904"/>
            <dbReference type="Rhea" id="RHEA-COMP:13703"/>
            <dbReference type="Rhea" id="RHEA-COMP:13704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="25">Interacts with MAPT/TAU.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="EC" id="2.7.11.26" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452GMI3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003030"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003470.1">
        <property type="protein sequence ID" value="ENSGAGP00000003030.1"/>
        <property type="gene ID" value="ENSGAGG00000002421.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12196">
        <property type="entry name" value="MARK1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14072">
        <property type="entry name" value="STKc_MARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14405">
        <property type="entry name" value="UBA_MARK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001032">
        <property type="entry name" value="KP78b, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000001">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049508">
        <property type="entry name" value="MARK1-4_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF21">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MARK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 28" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 28" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="30">
        <location>
            <begin position="60"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="31">
        <location>
            <begin position="330"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="32">
        <location>
            <begin position="744"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="375"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="538"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="378"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="447"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="588"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="645"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="659"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="681"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00056999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00061989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00070361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00083986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="793" mass="88985" checksum="E6BBF750CF7F2E33" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMJ2</accession>
    <name>A0A452GMJ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Profilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the profilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMJ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003041"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003481.1">
        <property type="protein sequence ID" value="ENSGAGP00000003041.1"/>
        <property type="gene ID" value="ENSGAGG00000002428.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032233">
        <property type="term" value="P:positive regulation of actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048278">
        <property type="entry name" value="PFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005455">
        <property type="entry name" value="PFN_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036140">
        <property type="entry name" value="PFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005454">
        <property type="entry name" value="Profilin1/2/3_vertebrate"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13936">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13936:SF5">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00235">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01639">
        <property type="entry name" value="PROFILINMAML"/>
    </dbReference>
    <dbReference type="SMART" id="SM00392">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55770">
        <property type="entry name" value="Profilin (actin-binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="125" mass="13805" checksum="ABD235124BCD710D" modified="2019-05-08" version="1">MSGWQEYISSLMRDGLCCDAAVISMAHQQLLAAHPGGVLENMTQLEIQALLDRNRDTLLTKGATLGGVKCLVIRDNLYTDTNNYTMDLRTKQQENMCLILVGQNGIQGGTLNLKAFKMRSQPLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMM4</accession>
    <name>A0A452GMM4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SANTA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GMM4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002897"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003314.1">
        <property type="protein sequence ID" value="ENSGAGP00000002897.1"/>
        <property type="gene ID" value="ENSGAGG00000002311.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039110">
        <property type="entry name" value="KNL2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015216">
        <property type="entry name" value="SANTA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16124">
        <property type="entry name" value="MIS18-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16124:SF3">
        <property type="entry name" value="MIS18-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09133">
        <property type="entry name" value="SANTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANTA" evidence="2">
        <location>
            <begin position="439"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="505" mass="56322" checksum="A1E95B1DF44A4EB9" modified="2019-05-08" version="1">MIITPLKNTRISKSHISSSRRGDMPLQAILMSNIPSGTITPLKDLVKFQDAGLTTSATKEAVLPSALRDFKENHARGAGTSKKREGIFQSTLITDGTYVKEFLDLSEIKPDSDTVAVPFASCQQQQILPLKDTNLLKRKACDPLTHESPAKIFQRMKAKASHEKQHQMPSKGTLLGTDYNSNLILTPATKLVHQGTQDKKSSDEENQQRAGKVPDRQIQPGKALHCTKIARNKNVDSLALDTLVSESPQKFFLRIKQKVQKQQKDPALSNQIKQSISSTIMNKPLIKSDFAKPVSNFNGECTVNNTSNSQDDVFLVEPIDADDEMSQNTMIDTVNTNADPSKTRAQLAERYGSGETIYASPHQEGRLLQERTWKTAQGVEKKSETDLQRPTQCFCSIMFSSPKVHIPRKQKPKEGDCKAPSSTSHIDKNDGTENKQSKICLSEWRIKVINNNTAVCIEGKRRDMKELYWHSNAIVERMASNQVKTISGSIYLLQGNIDSVSMRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GN47</accession>
    <name>A0A452GN47_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GN47"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003089"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003554.1">
        <property type="protein sequence ID" value="ENSGAGP00000003089.1"/>
        <property type="gene ID" value="ENSGAGG00000002486.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="64" mass="6949" checksum="2D547FC731C504AB" modified="2019-05-08" version="1">MPVLPFPASLHGAEPLFHDLDSDDTSLSNLGDCFLATSEAGPLQSRMGNPIDQLYSMQNSYFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNR0</accession>
    <name>A0A452GNR0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidoglycan binding-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GNR0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003519"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004057.1">
        <property type="protein sequence ID" value="ENSGAGP00000003519.1"/>
        <property type="gene ID" value="ENSGAGG00000002844.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF291">
        <property type="entry name" value="MATRIX METALLOPROTEINASE 1, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidoglycan binding-like" evidence="12">
        <location>
            <begin position="16"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="114" mass="13656" checksum="BA7CF5A611EC5DDA" modified="2019-05-08" version="1">EKGIRTHPESVSFYFQNYLDRFFPSIHKADSRTLEDKIKEMQKFFHLTVTGKFNSETEEMMDQPRCGIPDVLDYSTFPGNPRWRKNLLTYRLVIFYFLCILFSFNDAILFHTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPS0</accession>
    <name>A0A452GPS0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GPS0"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004531.1">
        <property type="protein sequence ID" value="ENSGAGP00000003899.1"/>
        <property type="gene ID" value="ENSGAGG00000003192.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="96" mass="10163" checksum="E0D3D8F3D89474D5" modified="2019-05-08" version="1">LQTHLPQESVTPGGTVTLSCSLSTGAITTSNYPSWYQQKLGSAPQQLIYSTNTRPTGIPARFSGSISSNNAVLTVTDVQAEDEADYYCAVFTGSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRU8</accession>
    <name>A0A452GRU8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GRU8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004589"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005375.1">
        <property type="protein sequence ID" value="ENSGAGP00000004589.1"/>
        <property type="gene ID" value="ENSGAGG00000003790.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001360">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF12">
        <property type="entry name" value="KEKKON 5, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="LRRCT" evidence="5">
        <location>
            <begin position="282"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="354" mass="38678" checksum="C470E3106C723717" modified="2019-05-08" version="1">MCLSRCTLLTGLTVHCQVGQGRAPHPELGGWGKSACNITQFVCVSPSLSNFPTGFPEETLMISVEFTNLSSIGPDALQKLPKLQELHLSSNQLRSIPGSLFRDLPALRVLDLTNNLLEDLPPEIINSYSPLQHLVLNGNRLAALEPAWFQTLGQLEWLDVSNNRLQALPPNCFQNLSSLKSLDLSNNHLDRLAPELLAGLPSLERLNLEGNRLHSIVEHTFDLVPHLSYLFLQHNNLSSLSGQLFWELGQLRHLDLSNNSLTSLAPCFSEQNLTLDLDLSGNPWACDCAMLSFVQWVTGHSVGLYASQYTLCGTPKNLKGQTVAAATKDKLTASCSAAPAERPSPCSLPRGQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTW5</accession>
    <name>A0A452GTW5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTW5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005382"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006267.1">
        <property type="protein sequence ID" value="ENSGAGP00000005382.1"/>
        <property type="gene ID" value="ENSGAGG00000004367.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051856">
        <property type="entry name" value="CSR-E3_Ligase_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012858">
        <property type="entry name" value="DC_STAMP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058842">
        <property type="entry name" value="DCST1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21041">
        <property type="entry name" value="DENDRITIC CELL-SPECIFIC TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21041:SF17">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE DCST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07782">
        <property type="entry name" value="DC_STAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26037">
        <property type="entry name" value="zf-RING_DCST1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Dendritic cell-specific transmembrane protein-like" evidence="5">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="E3 ubiquitin-protein ligase DCST1-like C-terminal" evidence="6">
        <location>
            <begin position="184"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="245" mass="28127" checksum="16E16AC0B060D456" modified="2019-05-08" version="1">MLELLECVPPLLFLLLAWGLDHVLYTMFSVIRHHSFVQYSFHSSHHLEVRVGGRSLLARLLRSTIGALNTSSEMALESTNLACLPQPQAMAQSDYLASCLPLGALVLLCLGQVYAHRLRRVIAAFYFPKREKKRVLFLYNQLLHERQAFVQLQRRRITRHARRHQPLLERLYLWCPLLRRCLRRYCVLCGSPESRASQLCPTPTCSTIYCQPCWRDMGQVCFACTPGHASLSGDSSSEEQMTYTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GU28</accession>
    <name>A0A452GU28_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">N-acyl-aromatic-L-amino acid amidohydrolase</fullName>
            <ecNumber evidence="9">3.5.1.114</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an N-acetyl-L-cysteine-S-conjugate + H2O = an S-substituted L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:36855"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:58717"/>
            <dbReference type="ChEBI" id="CHEBI:58718"/>
            <dbReference type="EC" id="3.5.1.114"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an N-acyl-aromatic L-alpha-amino acid + H2O = an aromatic L-alpha-amino acid + a carboxylate</text>
            <dbReference type="Rhea" id="RHEA:54184"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:84824"/>
            <dbReference type="ChEBI" id="CHEBI:138093"/>
            <dbReference type="EC" id="3.5.1.114"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Apical cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AspA/AstE family. Aspartoacylase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.114" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452GU28"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005545"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006450.1">
        <property type="protein sequence ID" value="ENSGAGP00000005545.1"/>
        <property type="gene ID" value="ENSGAGG00000004489.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004046">
        <property type="term" value="F:aminoacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06909">
        <property type="entry name" value="M14_ASPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000025">
        <property type="entry name" value="aspartoacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00704">
        <property type="entry name" value="Aspartoacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050178">
        <property type="entry name" value="AspA/AstE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016708">
        <property type="entry name" value="Aspartoacylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055438">
        <property type="entry name" value="AstE_AspA_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007036">
        <property type="entry name" value="Aste_AspA_hybrid_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002601">
        <property type="entry name" value="PRK02259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15162">
        <property type="entry name" value="ASPARTOACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15162:SF5">
        <property type="entry name" value="N-ACYL-AROMATIC-L-AMINO ACID AMIDOHYDROLASE (CARBOXYLATE-FORMING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24827">
        <property type="entry name" value="AstE_AspA_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04952">
        <property type="entry name" value="AstE_AspA_hybrid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018001">
        <property type="entry name" value="Aspartoacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Succinylglutamate desuccinylase/Aspartoacylase catalytic" evidence="16">
        <location>
            <begin position="13"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="AstE/AspA barrel-sandwich hybrid" evidence="15">
        <location>
            <begin position="218"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="13">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR018001-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR018001-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF24827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="313" mass="35288" checksum="788072F9AEEDB946" modified="2019-05-08" version="1">MAASCHLKPLSCVAVCGGTHGNEMSGIYLAKHWLRDPMELQRSSFAVTPFLGNPRAIEKCVRYVGQDLNRTFTTEFLSSTEVEDEPYEVVLAREINQTYGPKGSAQAFDFMFDLHNTTASMGTYLIVDSEESLLPMHMCHYIQMHYTRSPCPIYLYKLPGEETYSINSVAKAGLALELGPQPQQVVRADLFSQMRDIIACALDFIELFNKGTVFPAFEIEAYKITGRMDYPCYPDGEISAMVHPKLQDKDFLPLNPRDPIFQTFSGEEILYEGNTTIYPAFINEAAYYKKKVAFVKMEKHTFSIPALSSSRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GU34</accession>
    <name>A0A452GU34_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GU34"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005564"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006474.1">
        <property type="protein sequence ID" value="ENSGAGP00000005564.1"/>
        <property type="gene ID" value="ENSGAGG00000004509.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF11">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="369"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="207"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="429"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="474"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="508"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="520" mass="57663" checksum="071C4C46D9F73B52" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVY8</accession>
    <name>A0A452GVY8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GVY8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006217"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007228.1">
        <property type="protein sequence ID" value="ENSGAGP00000006217.1"/>
        <property type="gene ID" value="ENSGAGG00000005001.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21318">
        <property type="entry name" value="CH_SPTBN4_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21322">
        <property type="entry name" value="CH_SPTBN4_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000003">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000004">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="54"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="173"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="396" mass="44763" checksum="D97388176C234DDE" modified="2019-05-08" version="1">MANGAAELDNMEVQLQNNNTRWENMDNQAWDDTTATAKLFECSRIKALADEREAVQKKTFTKWVNSHLAQVSCRISDLYADLRDGYMLTKLLEVLSGEQLPKPTRGRMRIHSLENVDKALQFLKEQRVHLENVGSHDIVDGNHRLTLGLIWTIILRFQIQVIKIETEDNRETRSAKDALLLWCQMKTAGYPEVNIQNFTTSWRDGLAFNALIHRHRPEIIDFSKLSKSNATYNLQQAFNVAEQQLGVTKLLDPEDVNMESPDEKSIITYVVSFYHYFSKMKALAVEGKRIGKVLDQAVEMEKLMERYELLAAELLAWIEHTVGIISNQKFANSLAGVQQQLQAFTTFCTLQKPVKGALRRGCSPPDSSGCRQSGAVVGRGFGSGVSIPSWLQESLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVZ7</accession>
    <name>A0A452GVZ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the epsin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GVZ7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006225"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007236.1">
        <property type="protein sequence ID" value="ENSGAGP00000006223.1"/>
        <property type="gene ID" value="ENSGAGG00000005010.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000007238.1">
        <property type="protein sequence ID" value="ENSGAGP00000006225.1"/>
        <property type="gene ID" value="ENSGAGG00000005010.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16990">
        <property type="entry name" value="ENTH_Epsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000002">
        <property type="entry name" value="epsin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF50">
        <property type="entry name" value="EPSIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ENTH" evidence="8">
        <location>
            <begin position="12"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="161"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="276"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="406"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="570"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="410"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="582"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="592" mass="63809" checksum="6575F6106E5B06F1" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GX74</accession>
    <name>A0A452GX74_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Macrophage mannose receptor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000006732"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007819.1">
        <property type="protein sequence ID" value="ENSGAGP00000006732.1"/>
        <property type="gene ID" value="ENSGAGG00000005403.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23407">
        <property type="entry name" value="beta-trefoil_Ricin_MRC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00062">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000001">
        <property type="entry name" value="Fibronectin 1a isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000032">
        <property type="entry name" value="macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000014">
        <property type="entry name" value="Macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000023">
        <property type="entry name" value="Macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000031">
        <property type="entry name" value="macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000025">
        <property type="entry name" value="Mannose receptor C-type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000027">
        <property type="entry name" value="Mannose receptor, C type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.10">
        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000562">
        <property type="entry name" value="FN_type2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036943">
        <property type="entry name" value="FN_type2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24562">
        <property type="entry name" value="CysR_MRC2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00040">
        <property type="entry name" value="fn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00013">
        <property type="entry name" value="FNTYPEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00059">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51092">
        <property type="entry name" value="FN2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1293"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="16">
        <location>
            <begin position="155"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="213"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="365"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="474"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="620"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="771"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="909"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="1062"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="1166"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1349" mass="154702" checksum="5019E92EBDA802AF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXF2</accession>
    <name>A0A452GXF2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXF2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006824"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007919.1">
        <property type="protein sequence ID" value="ENSGAGP00000006824.1"/>
        <property type="gene ID" value="ENSGAGG00000005496.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000233">
        <property type="entry name" value="Putative zinc finger protein 362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000158">
        <property type="entry name" value="Zinc finger protein 362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000377">
        <property type="entry name" value="zinc finger protein 362 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000216">
        <property type="entry name" value="Zinc finger protein 384 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="372"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="183"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="481" mass="52102" checksum="6C1C67FF676F2041" modified="2019-05-08" version="1">MGSASAEGPTACWEACSSRSLPPLSPMQSQRWGHGWEHPSPRHTWTCRGGAMPQNSGIVGGGSQCLLTCTVPGLAASLWGWYLLCSCASGSDVCVLLSSSQLDNLVLINKIKEQLMAEKIRPPHLPPTSVSSQQPLLVPPSPAESSQSIMSLPKLQQVPGLHPQAVPQPDVALHARPATSTVTGLGLASRATAVSTSESSTGTSTPSTPTSTSQSRLIASSPTLISGITSPPLLDSIKTIQGHSLLGAPKPERGRKKIKAENPSGPPVLVVPYPILASGETAKEGKTYRCKVCPLTFFTKSEMQIHSKSHTEAKPHKCPHCSKSFANASYLAQHLRIHLGVKPYHCSYCEKSFRQLSHLQQHTRIHTGDRPYKCPHPGCEKAFTQLSNLQSHQRQHNKDKPYKCPNCYRAYSDSASLQIHLSAHAIKHAKAYCCSMCGRAYTSETYLMKHMSKHTVVEHLVSQHSPQRTESPGIPVRISLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GY82</accession>
    <name>A0A452GY82_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Coiled-coil domain containing 85A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCDC85 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GY82"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007117"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008239.1">
        <property type="protein sequence ID" value="ENSGAGP00000007117.1"/>
        <property type="gene ID" value="ENSGAGG00000005722.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019359">
        <property type="entry name" value="CCDC85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13546:SF14">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 85C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13546">
        <property type="entry name" value="RE60986P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="51" mass="5835" checksum="776BDEB06FE82FF2" modified="2019-05-08" version="1">MQDNYEEDLSEKEKAIVREMCNVVWRKLGDAASSKPSIRQHLSGNQFKGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYE2</accession>
    <name>A0A452GYE2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MGT5A-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GYE2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007219"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008348.1">
        <property type="protein sequence ID" value="ENSGAGP00000007219.1"/>
        <property type="gene ID" value="ENSGAGG00000005798.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000040374.1">
        <property type="protein sequence ID" value="ENSGAGP00000035655.1"/>
        <property type="gene ID" value="ENSGAGG00000025316.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042759">
        <property type="entry name" value="CCDC126"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027833">
        <property type="entry name" value="MGT5A-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46941">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46941:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15027">
        <property type="entry name" value="MGT5A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="MGT5A-like N-terminal" evidence="4">
        <location>
            <begin position="4"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="140" mass="15646" checksum="B4A8EC2F066CD511" modified="2019-05-08" version="1">MFLTFSRKNMSQKLSLLLLVFGFIWGLMLLRYTFQHPRHQSSAELREQILDLSKRYVKALAEENKNIMNGGNGASMAGYADLKRTIAVLLDDILQRLVKLENKVDYIVVNGSATNTTNGTSGNLVPVTTSKRVNAASNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H047</accession>
    <name>A0A452H047_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DEP domain-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEPDC7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H047"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007793"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008982.1">
        <property type="protein sequence ID" value="ENSGAGP00000007793.1"/>
        <property type="gene ID" value="ENSGAGG00000006209.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04446">
        <property type="entry name" value="DEP_DEPDC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04405">
        <property type="entry name" value="RhoGAP_BRCC3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16206">
        <property type="entry name" value="DEP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16206:SF9">
        <property type="entry name" value="DEP DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="46"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="520" mass="59170" checksum="91EE3D9471517C9A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H357</accession>
    <name>A0A452H357_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H357"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009056"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010394.1">
        <property type="protein sequence ID" value="ENSGAGP00000009056.1"/>
        <property type="gene ID" value="ENSGAGG00000007149.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006700">
        <property type="term" value="P:C21-steroid hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042359">
        <property type="term" value="P:vitamin D metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20646">
        <property type="entry name" value="CYP27A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000006">
        <property type="entry name" value="Cytochrome P450 302a1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF123">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 27 SUBFAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="440" mass="50463" checksum="CF58D347451829CA" modified="2019-05-08" version="1">MFRKDYGPMWKSTFGKYKNVNIGSPELLEQLLRQEGKYPMRSDMALWKEHRDTRQLPYGPLTEEGERWYRLRQVLNQRMLKPSEAMLYAGAINEVVSDLMARLQAERVGSPSGVLVEDMANLLYRFALEGISYILFETRTGCLEREIPAETRRFIDAIGLMLKNSVYATVMPQWSRKLLPFWRRYLENWDTIFAFGKKLIDRKMEELEGHLERGEQVSGYLSYLLASGRLTPEEVYGSVAELLMAGVDTTSNTLSWALYHLARAPEIQISLYQEVNSVVPRERIPSAKDLAKMPLLRAIIKETLRLYPVVPTNARVIVENDVVIGGYHFPKNTLFVLAHYAMAHDQSSFPEPERFLPQRWLRGHGSAHHPFSSIPFGYGVRGCLGRRIAELEMHLALARIARTFELRPDPQGTEVTSVSRIVLVPNKPINLQFIARQPGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H5X1</accession>
    <name>A0A452H5X1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H5X1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009931"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011404.1">
        <property type="protein sequence ID" value="ENSGAGP00000009931.1"/>
        <property type="gene ID" value="ENSGAGG00000007809.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000647">
        <property type="entry name" value="myeloid zinc finger 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000384">
        <property type="entry name" value="Zinc finger protein 550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002134">
        <property type="entry name" value="Zinc finger protein 616"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000431">
        <property type="entry name" value="zinc finger protein 629 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000953">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000862">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001489">
        <property type="entry name" value="Zinc finger protein interacting with ribonucleoprotein K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF152">
        <property type="entry name" value="SI:DKEY-210J14.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="23"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="126"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="514"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="542"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="570"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="598"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="626"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="653" mass="74760" checksum="306623E4F065110D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H761</accession>
    <name>A0A452H761_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Coagulation factor X</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H761"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010525"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012085.1">
        <property type="protein sequence ID" value="ENSGAGP00000010525.1"/>
        <property type="gene ID" value="ENSGAGG00000008234.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000013">
        <property type="entry name" value="Coagulation factor X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000162">
        <property type="entry name" value="Coagulation factor X (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012224">
        <property type="entry name" value="Pept_S1A_FX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF28">
        <property type="entry name" value="COAGULATION FACTOR X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001143">
        <property type="entry name" value="Factor_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="51"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="187"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="147"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001143-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="47204" checksum="E800116DC01EA5BE" modified="2019-05-08" version="1">RCWRTKSSCGLLMPGKETEVRGECVSACLDFREVHGVEEDAGMLWNYSIQNGDQCKPNPCQYGGTCKDGIGTYTCTCLDGYQGKNCESVIPKYCKLNNGDCDHFCRPIRNDVECFCMKQCTDKDGFSHCFDSERKLDLSVSSFANSSSVTSEQDDSPDLHLQNETKAPDRKETDPKTNVSPNPHTRIVGGDDCLPGECPWQALLINENKEGFCGGTILSQFYILTAAHCINQSKIIKVVVVDRDKEENTEMMYSVEKIFVHGRFVSATYDYDIALIKLKEPIKFSIYVIPACLPDADFANEILMNQKSGTVSGFGRLYEHGRVSNKLKVLQVPYIDRNSCKQITNFAITENMLCAGYDNITKDACQGDSGVPHVTKYKDTYFVTGIVSWGEGCARKGKYGVYTKVSKFHAWLKRIFRLLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9M9</accession>
    <name>A0A452H9M9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H9M9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011466"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013127.1">
        <property type="protein sequence ID" value="ENSGAGP00000011466.1"/>
        <property type="gene ID" value="ENSGAGG00000008846.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF142">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="12"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="147" mass="16666" checksum="DF004C3D026F1E94" modified="2019-05-08" version="1">KPVPDSIFPLPVIFEEVAVKLTRKEWTLLDPTQRALYWDVMQENYKDVTSLGKGSCPSVLGRGNEELRLTPAPRCPLCSVLFQHHPNMPVTHTHATRDPSLLQNTLRTEHRSHIAQLAGNLVAHLVSFRTQREQSKTQKTQTKASLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HA08</accession>
    <name>A0A452HA08_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase inhibitor 16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May inhibit cardiomyocyte growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with PSP94/MSMB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HA08"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011515"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013184.1">
        <property type="protein sequence ID" value="ENSGAGP00000011515.1"/>
        <property type="gene ID" value="ENSGAGG00000008889.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05559">
        <property type="entry name" value="CAP_PI16_HrTT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000011">
        <property type="entry name" value="Peptidase inhibitor 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019077745" description="Peptidase inhibitor 16" evidence="11">
        <location>
            <begin position="22"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="12">
        <location>
            <begin position="25"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="223"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="353"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="241"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="296"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="374"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="487" mass="52467" checksum="83E28B9AAEF31E38" modified="2019-05-08" version="1">MLSSGLPPLLLLLTAVELSWSLTDDDKRLIVEMHNHYRSQVSPHAADMLKMSWASDLEAFAKAYAAKCIWGHNKERGRRGENLFAITDEMDVEVALEQWYDEHKHYNLTTSKCTVGQMCGHYTQVVWANSEHVGCGMQFCKTLHGVEEPDLYLLVCNYDPPGNVRGRKPYKEGPPCSKCPEGYTCRNSICEPSVDSEEASASSRTTRPNLITRPALTTATMSPTTTATITPPPTTVKKRLAPTTTTRPAPTTTSPTTTTTLGPAPTTTTATTRLNPTMTTRLSPTTTPTTNPPPIRTARPNPTTATGPTSSLEPTSDLGRKTSPEMQVDSEETGAPLVTLEAVLFLDLESTLSPTASPEMEGDLKESDTASVRAEPALSSEPPPSFSLTTLETNLSLEDTKQPPPALKPAPATAKEISLLHLLPPSKAAGGHSLTIKSSLSGARETEELMTGPTKKDLDQQNGSATSNLLEFSLFLMAAPLLTGLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HA69</accession>
    <name>A0A452HA69_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HA69"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011605"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013300.1">
        <property type="protein sequence ID" value="ENSGAGP00000011605.1"/>
        <property type="gene ID" value="ENSGAGG00000008967.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000013303.1">
        <property type="protein sequence ID" value="ENSGAGP00000011608.1"/>
        <property type="gene ID" value="ENSGAGG00000008967.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035735">
        <property type="term" value="P:intraciliary transport involved in cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029602">
        <property type="entry name" value="IFT74"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31432">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 74 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31432:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 74 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="304"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="455"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="598" mass="69120" checksum="F57ECA1AE98519AA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBY6</accession>
    <name>A0A452HBY6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Netrin receptor UNC5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the unc-5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HBY6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012286"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014071.1">
        <property type="protein sequence ID" value="ENSGAGP00000012286.1"/>
        <property type="gene ID" value="ENSGAGG00000009452.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005042">
        <property type="term" value="F:netrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033564">
        <property type="term" value="P:anterior/posterior axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000001">
        <property type="entry name" value="Unc-5 netrin receptor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000002">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000008">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.30:FF:000003">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000037">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000039">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037936">
        <property type="entry name" value="UNC5A-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057755">
        <property type="entry name" value="UNC5A-D-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033772">
        <property type="entry name" value="UPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582">
        <property type="entry name" value="NETRIN RECEPTOR UNC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582:SF4">
        <property type="entry name" value="NETRIN RECEPTOR UNC5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25609">
        <property type="entry name" value="Unc5_NetrinR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17217">
        <property type="entry name" value="UPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00218">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 14" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="325"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="123"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="ZU5" evidence="16">
        <location>
            <begin position="462"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="863" mass="94468" checksum="43610F237E03B597" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDR3</accession>
    <name>A0A452HDR3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome c oxidase subunit 6C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cytochrome c oxidase polypeptide VIc</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of 14 subunits. The complex is composed of a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in the mitochondrial DNA, and 11 supernumerary subunits COX4I, COX5A, COX5B, COX6A, COX6B, COX6C, COX7A, COX7B, COX7C, COX8 and NDUFA4, which are encoded in the nuclear genome. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the cytochrome c oxidase subunit 6c family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HDR3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012952"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014845.1">
        <property type="protein sequence ID" value="ENSGAGP00000012952.1"/>
        <property type="gene ID" value="ENSGAGG00000009938.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22901">
        <property type="entry name" value="CcO_VIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.93.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.93.10">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIc/VIIs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051389">
        <property type="entry name" value="Cytochrome_c_oxidase_VIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034884">
        <property type="entry name" value="Cytochrome_c_oxidase_VIc/VIIs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037169">
        <property type="entry name" value="Cytochrome_c_oxidase_VIc_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48416">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48416:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02937">
        <property type="entry name" value="COX6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81415">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="79" mass="8931" checksum="C93B938679AFF61B" modified="2019-05-08" version="1">MSSTLLPKPQMRGLLGKRLRFHIVGALIVSLGSAVLYKYGVAEPRKRAYADFYKNYDSMKDFEAMREAGVFESVQPKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFH5</accession>
    <name>A0A452HFH5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFH5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013608"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015576.1">
        <property type="protein sequence ID" value="ENSGAGP00000013608.1"/>
        <property type="gene ID" value="ENSGAGG00000010382.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000103">
        <property type="entry name" value="Histone H2A type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="15">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="124" mass="13983" checksum="24E2F9EE4990763C" modified="2019-05-08" version="1">MARFIMEKKFSSSPFNHAGLQFPVGRVHRLLRKGNYAERVGAGAPVYMAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGVCVTSFSLKSIYYENIDSNFWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFJ0</accession>
    <name>A0A452HFJ0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFJ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013610"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015578.1">
        <property type="protein sequence ID" value="ENSGAGP00000013610.1"/>
        <property type="gene ID" value="ENSGAGG00000010384.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000015583.1">
        <property type="protein sequence ID" value="ENSGAGP00000013615.1"/>
        <property type="gene ID" value="ENSGAGG00000010384.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097194">
        <property type="term" value="P:execution phase of apoptosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043525">
        <property type="term" value="P:positive regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00032">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1470:FF:000002">
        <property type="entry name" value="Caspase-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000001">
        <property type="entry name" value="Caspase-3 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033139">
        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016129">
        <property type="entry name" value="Caspase_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454">
        <property type="entry name" value="CASPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454:SF31">
        <property type="entry name" value="CASPASE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Caspase family p20" evidence="15">
        <location>
            <begin position="71"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="14">
        <location>
            <begin position="217"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="309" mass="34938" checksum="1A02830400C486D3" modified="2019-05-08" version="1">MEGDQSSPVPAQDEDQDGDQADTVDARPDRSDRFSFFGKKKNGPEQQPEAPVSNQCRIVNPTFKYNMDYKKVGKCIIINNKNFEHQTGMGTRNGTDKDAGELSKCFRSLGFEVVTYNDRRCDDMKKLLKQAADENHSDAACFACIFLSHGEDGFIYGTDGMMEIKNLTTFFRGDKCRTLIGKPKLFFIQACRGTTFDDGIQTDSGLADDTEVTDANPRYKIPVEADFLLAYSTVSGYFSWRNPGRGSWFVQSLCSILNDHGKQLEIMQILTRVNYMVATNYESQSDDPRYSEKKQIPCVVSMLTKELYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFM7</accession>
    <name>A0A452HFM7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phosphatidylinositol 4-kinase type 2</fullName>
            <ecNumber evidence="19">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:19878"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 19">Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A452HFM7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013651"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015623.1">
        <property type="protein sequence ID" value="ENSGAGP00000013651.1"/>
        <property type="gene ID" value="ENSGAGG00000010407.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039756">
        <property type="entry name" value="Lsb6/PI4K2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865:SF6">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE 2-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="21">
        <location>
            <begin position="123"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU367084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="484" mass="54735" checksum="92499A33D99A2A85" modified="2019-05-08" version="1">MAEPGPEEPDERLLLLSEPALQDSPPSRGLHGKAAPGGAVRLGGAPGLEEDGNVDTDPEEDGEEEPLLGASATRRSRKASASWDRETRIAAGNVGSTAEMNLFLDDPEFAEIVLRTEQAIECGVFPERISQGSSGSYFVKDPKKKTIGVFKPKSEEPYGHLNPKWTKYFHKICCPCCFGRGCLVPNQGYLSETGAYLVDTKLGLGVVPKTKVVWLVSETFNYSAIDRAKSRGKKYALEKVPKVGKKFHRIGLPPKVGSFQLFVEGYKEAEYWLRKFETDPLPENTRKQFQSQFERLVVLDYVIRNTDRGNDNWLVRYEKQDDDIDLSDKDIQWTTSKEPVIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLSQAKVPFSQETRDLILPRISDMNFVQDLCEDLYELFKTDKGFDKATFENQMSVMRGQILNLTQALKDGRSPIQLVQMPRVVVERNSTGSQGRIVHLSNAFTQTFHSRKPFFSSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFT0</accession>
    <name>A0A452HFT0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Hyccin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFT0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013704"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015697.1">
        <property type="protein sequence ID" value="ENSGAGP00000013704.1"/>
        <property type="gene ID" value="ENSGAGG00000010450.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018619">
        <property type="entry name" value="Hyccin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31220:SF3">
        <property type="entry name" value="HYCCIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31220">
        <property type="entry name" value="HYCCIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09790">
        <property type="entry name" value="Hyccin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="331"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="531" mass="58706" checksum="05B7479A4FC878D9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFW7</accession>
    <name>A0A452HFW7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">P-type ATPase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFW7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013732"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015726.1">
        <property type="protein sequence ID" value="ENSGAGP00000013732.1"/>
        <property type="gene ID" value="ENSGAGG00000010467.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF78">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE IK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="9">
        <location>
            <begin position="62"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="533" mass="61007" checksum="94DD2CAC3FAFA9C3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFX9</accession>
    <name>A0A452HFX9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vasculin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GC-rich promoter-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Functions as a GC-rich promoter-specific transactivating transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the vasculin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFX9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013768"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015766.1">
        <property type="protein sequence ID" value="ENSGAGP00000013768.1"/>
        <property type="gene ID" value="ENSGAGG00000010496.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028128">
        <property type="entry name" value="Vasculin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14339">
        <property type="entry name" value="VASCULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14339:SF11">
        <property type="entry name" value="VASCULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15337">
        <property type="entry name" value="Vasculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="308"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="443" mass="50189" checksum="CC5AEC74C94E3111" modified="2019-05-08" version="1">MAQHDFAPAWLNFPTPPSSTKSSLNFEKHSENLSWTENRYEANRRRHNSSDGFDPSIGRPNGGHFGRKEKNGWRSQGRNGTENIHHRGGGYHSGGSRSRTSTFHSGKSQGLLHENNVPDNETGKKEDKEEPKQFEAEDFPSLNPEYEREPNQNKSLAAGVWEYPLNPKSRSPRMLVIKKGNTKDLQISGFPVVGGLHSQPVKNGTGTSVYKGLVPKPATPPAKPAQWKSQTKENKLGTPFSHESSYGIGNFNAFKSTAKTFSVSQNSVKECNRSNSSSPVDKLNQPRLTKLTRMRTDKKSEFLKALKQDRVEEEHEDENHVGEEKDDSFNLHNSNNSHHERDINRNFENEIPQENGNASVISQQIIRSSTFPQTDVLSSSLEAEHRLLKEMGWQEDSENDETCAPLTEDEMREFQVISEQVILTKNYQALHFLFVYTLCFKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGB5</accession>
    <name>A0A452HGB5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Interleukin family protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Immune regulatory cytokine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the IL-10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGB5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013906"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015918.1">
        <property type="protein sequence ID" value="ENSGAGP00000013906.1"/>
        <property type="gene ID" value="ENSGAGG00000010592.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020443">
        <property type="entry name" value="IL-10/19/20/24/26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020444">
        <property type="entry name" value="IL-24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48482">
        <property type="entry name" value="INTERLEUKIN-19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48482:SF1">
        <property type="entry name" value="INTERLEUKIN-26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00726">
        <property type="entry name" value="IL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01937">
        <property type="entry name" value="INTRLEUKIN24"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="125" mass="14731" checksum="2826B52F95146D96" modified="2019-05-08" version="1">MILYSLFRSGLLLVLLLYVHFYLFTDVSKNCSVRDQLLSFYMKNVFGGLSIGSDKVYIVSAFQTLQENLSNCVSIFFEKKNRYLRKDLTLAFRIYYRLGEKGIYKAISELDILLHWIQTYIETIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHI8</accession>
    <name>A0A452HHI8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial import receptor subunit TOM7 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Translocase of outer membrane 7 kDa subunit homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HHI8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014349"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016418.1">
        <property type="protein sequence ID" value="ENSGAGP00000014349.1"/>
        <property type="gene ID" value="ENSGAGG00000010909.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903955">
        <property type="term" value="P:positive regulation of protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012621">
        <property type="entry name" value="Tom7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46722">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46722:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08038">
        <property type="entry name" value="Tom7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="66" mass="7625" checksum="AFADB2F46679EB14" modified="2019-05-08" version="1">MPRLSKEAKQRLQQLFKGGQFAIRWGFIPVVLYLGFKRGADPGMPEPTILRYVYICKYTCKNNAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HI80</accession>
    <name>A0A452HI80_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HI80"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014601"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016702.1">
        <property type="protein sequence ID" value="ENSGAGP00000014601.1"/>
        <property type="gene ID" value="ENSGAGG00000011093.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007208">
        <property type="term" value="P:phospholipase C-activating serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007210">
        <property type="term" value="P:serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF32">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="80"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="60"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="146" mass="15471" checksum="A47FFCFD456C9AE0" modified="2019-05-08" version="1">HLLLKHGVTSFLTHLRYSPSLPTSDPLNASEWGEVSHMSMREKNWPALLILVIILLTIGGNILVIMAVSLEKKLQNATNYFLMSLAVADMLVGILVMPVSLITILYGLVPSVTYVGINTGVDSSPANIASASCGGGFSLCNASSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIB0</accession>
    <name>A0A452HIB0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HIB0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014597"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016697.1">
        <property type="protein sequence ID" value="ENSGAGP00000014597.1"/>
        <property type="gene ID" value="ENSGAGG00000011091.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF133">
        <property type="entry name" value="RIKEN CDNA 1700020N01 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019071864" description="KRAB domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="2">
        <location>
            <begin position="19"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="151" mass="16793" checksum="50C2DAD977ACCEA6" modified="2019-05-08" version="1">IPPGSLPTRIGILLFQGLVTFAEVAVYFTREEGALLDPTQRALYRGVMQENYETVTSLGKGSFSKPYVISQLEGGEEPWVLDLPGSEESEILRSPCTGEETSNQPRNCKCLREPAGILHTDFMSSGYFSQSSGLLYHQRIRKGDKLHKNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLM8</accession>
    <name>A0A452HLM8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2 Cu(+) + NADP(+) + H(+) = 2 Cu(2+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:71771"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:71773"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>2 Fe(2+) + NADP(+) + H(+) = 2 Fe(3+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:71767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:71769"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the STEAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HLM8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015835"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018091.1">
        <property type="protein sequence ID" value="ENSGAGP00000015835.1"/>
        <property type="gene ID" value="ENSGAGG00000011914.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008823">
        <property type="term" value="F:cupric reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000051">
        <property type="entry name" value="STEAP2 metalloreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051267">
        <property type="entry name" value="STEAP_metalloreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239">
        <property type="entry name" value="DUDULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239:SF5">
        <property type="entry name" value="METALLOREDUCTASE STEAP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="17 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="292"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="22">
        <location>
            <begin position="22"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric oxidoreductase" evidence="21">
        <location>
            <begin position="249"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="472" mass="53511" checksum="DF5A6DD758ADE088" modified="2019-05-08" version="1">MEKNSSNMIPLTENSPPKKEMVCIFGTGDFGRSLGYKMLQCGYSIAFGSRNAQTSSLIPKGTEVLSHREAAQKADIIIVAIQRQHYNFLTELTEVLHGKVLVDVSNNLKINQYPESNAEYLAQLVPGAKVVKAFNTVSAWALQSGTLDASRQVFVCGDDNKAKQMVMDIVRTLGLTPLDQGSLLAAREIENYPLQLFPMWKFPIYLAIGLSAFFFFYSLIRDVIYPCIYENKDLSFFIAISIPNRVCPIVALILLALVYLPGIIAAILQLHRGTKYSRFPDWLDKWMLCRKQLGLVALVFAFLHVLYTLVIPIRYYVRWRLNVYVISQIKDNRTDPFNNTNGWISDSYLALGILGFFLFVLLGITSLPSVSNSVNWREFRFVQSKLGYLTLILCTAHTLVFGGKRFLNPSMYRWYLPAAYMLSLIVPCIVLAIKFVLIFPCIDRPLTQIRQGWERSSKGSKQSYYNDSKSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HM81</accession>
    <name>A0A452HM81_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 45B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM45 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HM81"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018340.1">
        <property type="protein sequence ID" value="ENSGAGP00000016056.1"/>
        <property type="gene ID" value="ENSGAGG00000012019.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006904">
        <property type="entry name" value="DUF716"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042127">
        <property type="entry name" value="TMEM45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16007">
        <property type="entry name" value="EPIDIDYMAL MEMBRANE PROTEIN E9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16007:SF21">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 45A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04819">
        <property type="entry name" value="DUF716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="31929" checksum="BBE04B28872E741E" modified="2019-05-08" version="1">YELPNCNSTEFSHPIELKQIFKGNALRGTFFLIFGLGWSVKYPLKYLSRKLNADNQASRWIEKLDILEGAAKAFLAVAGMLAEQFVPDGPHMGLYSGGTYSWEKLTNWQYTTMYLFYGLSGIVDVLSYSPLKLSLGLDRLLLSVAMFTEGFLFCFHDYNNTALDQHLHSLLLIAIFGGALCALMEVFLRDHTILEFFRTSFFILQGSWFWQIGFVLSPPWGGPGWDQTDRGNFNFLTMCFFWHYAAALLVTAANSVFSYWYVVGQGSGMPGLDNHLCLLDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HN34</accession>
    <name>A0A452HN34_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HN34"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016385"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018699.1">
        <property type="protein sequence ID" value="ENSGAGP00000016385.1"/>
        <property type="gene ID" value="ENSGAGG00000012273.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000037">
        <property type="entry name" value="Transmembrane protease serine 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000173">
        <property type="entry name" value="Transmembrane protease serine 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF27">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15494">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 8" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="119"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="240"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="477" mass="52667" checksum="76469D404D199BBA" modified="2019-05-08" version="1">MISQEEVLESNPNTGNPENTSVTEDKLPVAEVHFLYKRFFSIQQAKVDPSANGLGAIQPSSKCHVLVSLRFLPFILVLLLVVILAVAIGLSVQYNCIGKFRCRSSFKCIQQSARCDGVFNCKEGEDEYTCVRLSGKKAVLQVFTFGSWRTVCSDNWNDENGNATCKRLGFSSYISSGYLPVAAIEEEFQRHFVSVSHWSSADQVTALHNASYFREECTSGNVIILKCLECGTRSRYSSRIVGGNASSPWQWPWQVSLQFQGFHLCGGSIITPWWIVTAAHCVYDLYSPSSWSVQVGFVTQQDISVNPYSVEKIIFHRNYKPKTMGNDIALMKLAAPLALNGLIEPICLPNFGEHFPEGKMCWISGWGATAEGDDTSETMNSAGVPLISNKVCNHRDVYGGIITSSMLCAGFLEGGVDTCQGDSGGPLACKDMNTWKLVGTTSFGMGCAEVNKPGVYSRTTSFLDWIHEQMEREELRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNG6</accession>
    <name>A0A452HNG6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HNG6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016539"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018874.1">
        <property type="protein sequence ID" value="ENSGAGP00000016539.1"/>
        <property type="gene ID" value="ENSGAGG00000012368.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000018876.1">
        <property type="protein sequence ID" value="ENSGAGP00000016541.1"/>
        <property type="gene ID" value="ENSGAGG00000012368.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22176">
        <property type="entry name" value="F-box_FBXO34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039594">
        <property type="entry name" value="FBXO34/46"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16271:SF11">
        <property type="entry name" value="F-BOX ONLY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16271">
        <property type="entry name" value="F-BOX ONLY PROTEIN 34/46 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="612"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="372"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="528"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="530"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="753" mass="83233" checksum="66A5DA344ECA32DB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTC2</accession>
    <name>A0A452HTC2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HTC2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018342"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020905.1">
        <property type="protein sequence ID" value="ENSGAGP00000018342.1"/>
        <property type="gene ID" value="ENSGAGG00000013272.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045260">
        <property type="entry name" value="Sec12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284:SF0">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3 11" id="KW-0853">WD repeat</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019163915" evidence="14">
        <location>
            <begin position="30"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="410"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="180"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="113"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="438" mass="47537" checksum="8A83DF8D6481DAF4" modified="2019-05-08" version="1">MHFQHLLGCGWFWAGLLRLVGQAVGLCCACPPRAAALEGAGAGCCWGAMAQLESAISGAHFLQLEQIGGQLSASLLHSHDTETRATMSMALAGDVIAAGQDASCHILRFRLQGPDEKSGSSTPSKAGETQSPRCWPRKGRPGWVPSEKGEGDTRSEAGEVAVEMLCSVQTDFSPDALQKAVRVSADHSLLATGGSDGFLRVWEFPSMKKVLEFKAHDGEIEDIALGPENKVVTAGRDFQCCVWQRDQLVLRLRWDENMPGVPDKTYRYQACRFGTVPDQAEALRLYTVQVPHKRGRRPPPCYLTKWDGKSFLPLLTQPCGGEVISCLSVSDSGTFLGLGTVTGSVAIHVAFSLQRLYYVKEAHGIVVTDVAFLPESRRGRELLAENEAALLSVAVDSRCKLHLLPPRRSFPVWLLLLLCTGLVVATILLLQVAFPGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTD8</accession>
    <name>A0A452HTD8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Scavenger receptor class B member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">SR-BI</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Caveola</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CD36 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HTD8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018355"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020919.1">
        <property type="protein sequence ID" value="ENSGAGP00000018355.1"/>
        <property type="gene ID" value="ENSGAGG00000013591.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030169">
        <property type="term" value="F:low-density lipoprotein particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033344">
        <property type="term" value="P:cholesterol efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070508">
        <property type="term" value="P:cholesterol import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034381">
        <property type="term" value="P:plasma lipoprotein particle clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043654">
        <property type="term" value="P:recognition of apoptotic cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005428">
        <property type="entry name" value="CD36/SCARB1/SNMP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF110">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01610">
        <property type="entry name" value="CD36ANTIGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01609">
        <property type="entry name" value="CD36FAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="467"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="467"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="483"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="250"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605428-53"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="507" mass="56537" checksum="BC5B42572B1C9A80" modified="2019-05-08" version="1">MAAPAGRGVPLALGLSGLLFALCGLVLLFVLPSLVRDQVIKNVRIDPNSFAFSMWKDIPVPFYLSAYLFEVMNPKEIRLGAKPVVNQRGPYVYREHRYKTNITFHDNGTVSFLEYRQFYFQPDLSDGREEDYIVVPNILVMGAAIMMENLPQLLKMMISGALAGLGQDAFMNRTVGEIMWGYDDPLIDVLNSFVPGLIPFKGKFGLFLNFNNSNTGLFTVYTGATDINQIHTVDTWNGLKKVNYWRSNECNMINGTSGEMWPPFMTPSSSLEFYSPDACRSMKLVYEQSGVFKGVPTYRFVAPKTLFANGTDYPPNEGFCPCRESGIQNVSTCRMNAPVFISHPHFYDADPILVEAVDGLHPNKEQHGLFLDIHPVTGIPMNCSIKLQLSLYIKKVSGISQTGQIKPVVLPMLWFAESGAIEGSVLQQFYTNLVLIPSVLEYVQYICIALGVALIVAAIVLEVKRQRNAREKSPREPDPQTEPPWSSETTPLLQDASKTNQNDAAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVP8</accession>
    <name>A0A452HVP8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Regulates the proliferation and migration of oligodendrocytes, a process essential for normal myelination and repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with claudin-11/CLDN11 and integrins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HVP8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019159"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021832.1">
        <property type="protein sequence ID" value="ENSGAGP00000019159.1"/>
        <property type="gene ID" value="ENSGAGG00000014167.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03163">
        <property type="entry name" value="TM4SF8_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000004">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF48">
        <property type="entry name" value="TETRASPANIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="260" mass="28564" checksum="50D7EC208EE260AB" modified="2019-05-08" version="1">MGTGAGAMGQCGITSSKTVLVFLNLIFWAAAGILCYVGAYVFITYDDYDHFFEDVYTLIPAVVIIAVGTLLFIIGLIGCCATIRESRCGLATFVIILLLVFITEVVVVVLGYIYRAKVEDEVDQSIQKVYDAYSGTNPDAATRAIDYVQRQLRCCGIHSYSDWQNTTWFKETKNNSVPLSCCKATSTNCTGSFTHPIDLYSEGCEALVVKKLQEIMMYVIWAALAFAAIQLLGMLCACVVLCRRSRDPAYELLITGGTYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWF1</accession>
    <name>A0A452HWF1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ERCC4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HWF1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019449"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022148.1">
        <property type="protein sequence ID" value="ENSGAGP00000019449.1"/>
        <property type="gene ID" value="ENSGAGG00000014344.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045003">
        <property type="term" value="P:double-strand break repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20077">
        <property type="entry name" value="XPF_nuclease_FANCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047418">
        <property type="entry name" value="XPF_nuclease_FANCM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025">
        <property type="entry name" value="FANCONI ANEMIA GROUP M FANCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="FANCONI ANEMIA GROUP M PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="6">
        <location>
            <begin position="263"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="154"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="447" mass="50127" checksum="9E7665C43A4ACA84" modified="2019-05-08" version="1">DISSDESDSENELNSSLNQFLNDETQFTEVLNDSEMEGVYLKSVCSPALGNRYKMVHRGFNSIAVFSQIPEQDEAYLADSFCVEEEEEESLRKSDSGEEEEACVNFDLVMDERSASGRKQYFTRRRVKLNQAKTEQNCAVPLTKKRSRIIVLDDSSGDETNVSNQRPIKTASPRTDYSHVHLPKSLPSDSPGQHNTPARKNPILQPQENKGQRLINLKTSVSEVLDFQPENRGRDTWCSASKGNSAAATPISAGLLERKASLCILVDSREISSGSEIISSLKAVHGVKVQVCSLGGCDYIVSNRMAVERRTQSELLNSMNRNRVTQRIQHLQSMFERICVIVEKDRIKAGETSRLFQRTKYYDAMLSALIRAGIRILFSSCQEESAHLLKDLVLVEQRKNVAICVPTEVKGAKEEALRFYLSIPNISYLAALNMCHGFASVKKMVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWT0</accession>
    <name>A0A452HWT0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin protein ligase</fullName>
            <ecNumber evidence="14">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the BRE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A452HWT0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019574"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022287.1">
        <property type="protein sequence ID" value="ENSGAGP00000019574.1"/>
        <property type="gene ID" value="ENSGAGG00000014430.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033503">
        <property type="term" value="C:HULC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16814">
        <property type="entry name" value="RING-HC_RNF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000040">
        <property type="entry name" value="E3 ubiquitin protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058643">
        <property type="entry name" value="BRE1-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058642">
        <property type="entry name" value="BRE1A/B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013956">
        <property type="entry name" value="E3_ubiquit_lig_Bre1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BRE1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163">
        <property type="entry name" value="RING FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26052">
        <property type="entry name" value="BRE1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26095">
        <property type="entry name" value="CC_Bre1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11 14 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="928"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="124"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="505"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="51"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="236"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="322"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="843"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="514"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="527"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="558"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="981" mass="114119" checksum="6FF32425B1A7FFAB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYJ0</accession>
    <name>A0A452HYJ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hematopoietic cell signal transducer</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DNAX-activation protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Membrane protein DAP10</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAP10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYJ0"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023066.1">
        <property type="protein sequence ID" value="ENSGAGP00000020244.1"/>
        <property type="gene ID" value="ENSGAGG00000014934.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050776">
        <property type="term" value="P:regulation of immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009861">
        <property type="entry name" value="HCST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21409">
        <property type="entry name" value="HEMATOPOIETIC CELL SIGNAL TRANSDUCER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21409:SF1">
        <property type="entry name" value="HEMATOPOIETIC CELL SIGNAL TRANSDUCER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07213">
        <property type="entry name" value="DAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="109" mass="11694" checksum="85503D43FC48446C" modified="2019-05-08" version="1">MAGCDPRAVCVCGGTEDGPWQGGRQGGGWAELPREEGSVELLLLPHPLVQTPGWDHGQGSTPVIVGLVIGDLVFTLVLILVVYHCTKRCGKPTDNKDQKVYMNMPGRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYS0</accession>
    <name>A0A452HYS0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sulfotransferase</fullName>
            <ecNumber evidence="13">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>3'-phosphoadenylyl sulfate + keratan = adenosine 3',5'-bisphosphate + keratan 6'-sulfate.</text>
            <dbReference type="EC" id="2.8.2.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>chondroitin beta-D-glucuronate + n 3'-phosphoadenylyl sulfate = chondroitin 6'-sulfate + n adenosine 3',5'-bisphosphate + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:11108"/>
            <dbReference type="Rhea" id="RHEA-COMP:9827"/>
            <dbReference type="Rhea" id="RHEA-COMP:9828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57652"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:62065"/>
            <dbReference type="EC" id="2.8.2.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:11109"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A452HYS0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020331"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023166.1">
        <property type="protein sequence ID" value="ENSGAGP00000020330.1"/>
        <property type="gene ID" value="ENSGAGG00000014990.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000023167.1">
        <property type="protein sequence ID" value="ENSGAGP00000020331.1"/>
        <property type="gene ID" value="ENSGAGG00000014990.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008459">
        <property type="term" value="F:chondroitin 6-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045130">
        <property type="term" value="F:keratan sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001517">
        <property type="term" value="F:N-acetylglucosamine 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000938">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016469">
        <property type="entry name" value="Carbohydrate_sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051135">
        <property type="entry name" value="Gal/GlcNAc/GalNAc_ST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704:SF60">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005883">
        <property type="entry name" value="Carbohydrate_sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="15">
        <location>
            <begin position="114"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="459" mass="52481" checksum="29EBA3CCB6AB8120" modified="2019-05-08" version="1">MDKGHALPQDIRDLRHCLRMKSKYAILLVFVVALVIIEKENNIISRVSDKLKQAPQPLMEVNSTDPGLVLSENGSLLSLSELDSAFSQLKNRLQNVTLQLGGTREPTVAQGPRRHVLLMATTRTGSSFVGEFFNQQGNIFYLFEPLWHIERTVSFEPGGANAVGSALVYRDVLKQLLLCDLYILENFITPFPEEHLTPYMFRRGSSRSLCEDPVCTPFVKKVFEKYHCKNRRCGPLNITLAAEACLHKNHMALKTVRIRQLEFLRPLVEDPRLDMRIIQLVRDPRAVLASRIVAFSGKYETWKKWASEGAAPLNEDEVQRLRGNCESIRLSAELGLRQPAWLRGRYMLIRYEDIARSPLQNAKEMYRFAGISLTPQVEEWIQKNTQAPQDSNGIYSTQKNSSEQFEKWRFSIPFKLAQVVQSVCGPAMSLFGYKLASDQETLTNRSISLLEERRAFWIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYX0</accession>
    <name>A0A452HYX0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bromodomain PHD finger transcription factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000020380"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023221.1">
        <property type="protein sequence ID" value="ENSGAGP00000020380.1"/>
        <property type="gene ID" value="ENSGAGG00000015012.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016589">
        <property type="term" value="C:NURF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15559">
        <property type="entry name" value="PHD1_BPTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000036">
        <property type="entry name" value="nucleosome-remodeling factor subunit BPTF isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038028">
        <property type="entry name" value="BPTF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028941">
        <property type="entry name" value="WHIM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45975">
        <property type="entry name" value="NUCLEOSOME-REMODELING FACTOR SUBUNIT BPTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45975:SF2">
        <property type="entry name" value="NUCLEOSOME-REMODELING FACTOR SUBUNIT BPTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00571">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50827">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DDT" evidence="9">
        <location>
            <begin position="251"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="401"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="582"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1074"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1257"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1346"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1670"/>
            <end position="1694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1989"/>
            <end position="2019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2059"/>
            <end position="2084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="141"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="201"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="582"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="603"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="629"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="642"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="701"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="753"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1080"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1125"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1257"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1352"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1400"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1415"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1431"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1449"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1683"/>
            <end position="1694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="2069"/>
            <end position="2084"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2305" mass="255779" checksum="D17E388E00B13236" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I060</accession>
    <name>A0A452I060_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CD99 antigen-like protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
            <orientation evidence="10">Extracellular side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD99 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I060"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020892"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023793.1">
        <property type="protein sequence ID" value="ENSGAGP00000020892.1"/>
        <property type="gene ID" value="ENSGAGG00000015361.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034109">
        <property type="term" value="P:homotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000391">
        <property type="term" value="P:positive regulation of neutrophil extravasation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072683">
        <property type="term" value="P:T cell extravasation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022078">
        <property type="entry name" value="CD99L2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15076:SF12">
        <property type="entry name" value="CD99 ANTIGEN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15076">
        <property type="entry name" value="CD99/MIC2 PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12301">
        <property type="entry name" value="CD99L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="34"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="283" mass="30026" checksum="2EC6C02BF314B85B" modified="2019-05-08" version="1">MRKANPYSTQKCRGNVLCIKLQCKLSGAGYGDDSDDFNLADALDDHPTKRPTPKLPKKPAGGTGYGDGLDDFDLSDALDDHPTKQPTPKLPRKPSGGTDISLWDVIHTTTTKKPKTTKAPPKKNPAKDPADFDLSDALDDQNDGKGGGKPNVKPGGGSKGKDNPSRGGKQAFSDDDLASVLDDGEYHPDKKKGGSDYDTGTTAETGTIAGIASALVMALIGAVSSYISYQQKKFCFSIQQGLNAEYVKGENTEAVVTEEPQVKYSVLETQSAEPPPPQDNAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1W9</accession>
    <name>A0A452I1W9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I1W9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021484"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024466.1">
        <property type="protein sequence ID" value="ENSGAGP00000021484.1"/>
        <property type="gene ID" value="ENSGAGG00000015771.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="370"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="433"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="465"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="494"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="726" mass="80173" checksum="179F6288785E965A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3Q2</accession>
    <name>A0A452I3Q2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRM HNRPC family. RALY subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3Q2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022147"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025245.1">
        <property type="protein sequence ID" value="ENSGAGP00000022147.1"/>
        <property type="gene ID" value="ENSGAGG00000016266.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12604">
        <property type="entry name" value="RRM_RALY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000019">
        <property type="entry name" value="heterogeneous nuclear ribonucleoproteins C1/C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034502">
        <property type="entry name" value="RALY_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051186">
        <property type="entry name" value="RRM_HNRPC/RALY_subfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968:SF6">
        <property type="entry name" value="RNA-BINDING PROTEIN RALY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="74"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="265"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="265"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="284"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="319" mass="35759" checksum="963A9FEF44F6A247" modified="2019-05-08" version="1">MKWWSCLLVCSVTKGCNPKLLLSLPPIFITFMLFPLACHTLPDSPDLFSQVFKMSLKVQTSNITNKNDPKSINSRVFIGNLNTAVVKKSDVETIFSKYGRVVGCSVHKGYAFVQYSNERHARAAVLGENGRILAGQTLDINMAGEPKPNRPKGLKRATSALYSGYDFDYDYYRDDFYDRLFEYRGRVSPVPRVVPVKRPRVTIPLVRRVKATLPVKLFARSAAIANSSAKLKLKCNELQTIKTELTQIKSNIDALLGRLEQIAEEQKSSTEIRKKADGNKSEISQDDTASEAEIITEEPLNGEEGEEEGLTRDDCEDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4Z6</accession>
    <name>A0A452I4Z6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium voltage-gated channel subfamily E regulatory subunit 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel KCNE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I4Z6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022594"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025740.1">
        <property type="protein sequence ID" value="ENSGAGP00000022594.1"/>
        <property type="gene ID" value="ENSGAGG00000016568.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902282">
        <property type="term" value="F:voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097623">
        <property type="term" value="P:potassium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086091">
        <property type="term" value="P:regulation of heart rate by cardiac conduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060307">
        <property type="term" value="P:regulation of ventricular cardiac muscle cell membrane repolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000369">
        <property type="entry name" value="K_chnl_KCNE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15282">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY E MEMBER 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15282:SF9">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY E MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02060">
        <property type="entry name" value="ISK_Channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00168">
        <property type="entry name" value="KCNECHANNEL"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="9843" checksum="605BAC0D7E29FB86" modified="2019-05-08" version="1">MLKMDHTNMTQTMLTAEPQTPEKNNSSGNEYFYILIVMSFYGIFLMGIMLGYMKSKRKEKKSNLLLLYKDEERCWGECIGAIGIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6L0</accession>
    <name>A0A452I6L0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dynein regulatory complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Coiled-coil domain-containing protein 65</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes. Plays a critical role in the assembly of N-DRC and also stabilizes the assembly of multiple inner dynein arms and radial spokes. Coassembles with DRC1 to form a central scaffold needed for assembly of the N-DRC and its attachment to the outer doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
            <location evidence="8">Cytoskeleton</location>
            <location evidence="8">Flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DRC2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I6L0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023205"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026440.1">
        <property type="protein sequence ID" value="ENSGAGP00000023205.1"/>
        <property type="gene ID" value="ENSGAGG00000017014.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003352">
        <property type="term" value="P:regulation of cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039505">
        <property type="entry name" value="DRC1/2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039750">
        <property type="entry name" value="DRC1/DRC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21625:SF0">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21625">
        <property type="entry name" value="NYD-SP28 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14772">
        <property type="entry name" value="NYD-SP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="4 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein regulatory complex protein 1/2 N-terminal" evidence="14">
        <location>
            <begin position="22"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="281"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="406"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="490" mass="57536" checksum="0D50F9C3BD8F6BAF" modified="2019-05-08" version="1">MPKKGRKAAALSEERLLLMQQKMLAEEELSKKKEDMLTQFLKDKLAKEEHNSALNLNKINLQWRTVLREVKARELRKDIEILSQTFERVVDCKDSVIQSLARDLSEAEAQYTHALCSHLHNIDQLLQLQRCRLSYLDEDYNTELETLKKGFETERRMIIEHHDKESRYLRDVLLALEQNFSESEYEAKLDFQSTRDDVKNKNLEEKHYLRVQLEGTVEELWKRFQQALKNYTEATEDRKIAFEALKLKDEKSSKEIEMQMKKLQKIQDSIGILKGKITAHMREAEEQNRRVHEEKEVVLKQLQKLKSQMNQARTKARSNLAKLTLESSAALKKLRGVLEKAELILRLAEMCRKLETEEEKVLPFYASLLSREEQEKVKQVSLEKAGEQLAQLMQDYLGLEHFWQRYNKVKLEQLSLEREKAVLRQENQKLRLLLKQYLDGISVSDEVLSRLNPLLIVNHKPRAPGPAGKAHRKRLLHNVIEAAHEVPHIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7E4</accession>
    <name>A0A452I7E4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TRASH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000023548"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026835.1">
        <property type="protein sequence ID" value="ENSGAGP00000023548.1"/>
        <property type="gene ID" value="ENSGAGG00000016088.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057926">
        <property type="entry name" value="QRICH1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010507">
        <property type="entry name" value="Znf_MYM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051284">
        <property type="entry name" value="ZnF_MYMT-QRICH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45736">
        <property type="entry name" value="ZINC FINGER MYM-TYPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45736:SF5">
        <property type="entry name" value="ZINC FINGER MYM-TYPE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25561">
        <property type="entry name" value="QRICH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24900">
        <property type="entry name" value="TRASH_ZMYM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06467">
        <property type="entry name" value="zf-FCS"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRASH" evidence="6">
        <location>
            <begin position="344"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="TRASH" evidence="6">
        <location>
            <begin position="391"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="TRASH" evidence="6">
        <location>
            <begin position="443"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="TRASH" evidence="6">
        <location>
            <begin position="488"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="TRASH" evidence="6">
        <location>
            <begin position="533"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="TRASH" evidence="6">
        <location>
            <begin position="581"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="TRASH" evidence="6">
        <location>
            <begin position="689"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="TRASH" evidence="6">
        <location>
            <begin position="731"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="TRASH" evidence="6">
        <location>
            <begin position="774"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="TRASH" evidence="6">
        <location>
            <begin position="818"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="859"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1142"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="868"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1161"/>
            <end position="1184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1376" mass="152737" checksum="852188FFA7F4B398" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7Z6</accession>
    <name>A0A452I7Z6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I7Z6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023756"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027055.1">
        <property type="protein sequence ID" value="ENSGAGP00000023756.1"/>
        <property type="gene ID" value="ENSGAGG00000017389.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000051">
        <property type="entry name" value="F-box and leucine-rich repeat protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000075">
        <property type="entry name" value="F-box/LRR-repeat protein 14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF273">
        <property type="entry name" value="F-BOX_LRR-REPEAT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="4">
        <location>
            <begin position="104"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="4">
        <location>
            <begin position="186"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="386" mass="42347" checksum="0229B938A0FFE1F4" modified="2019-05-08" version="1">MIFGYLEVRDKGRAAQVCTAWRDAAYHRSVWRGVEAKLHLRRANPSLFPSLAARGIRRVQILSLRRSLSYVIQGMAEIESLNLSGCYNLTDNGLGHAFVAEIGSLRALNLSLCKQITDSSLGRIAQYLKGLEVLELGGCSNITNTGLLLIAWGLQRLKSLNLRSCRHLSDVGIGHLAGMTRSAAEGCLGLEQLTLQDCQKLSDLSLKHLARGLGRLRQLNLSFCGGISDAGLLHLSHMSSLRSLNLRSCDNISDTGIMHLAMGSLRLSGLDVSFCDKVGDQSLAYIAQGLDGLRSLSLCSCHISDEGINRMVRQMHGLRTLNIGQCVRITDKGLELIAEHLSQLTGIDLYGCTRITKRGLERITQLPCLKVLNLGLWQMTESEKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8I1</accession>
    <name>A0A452I8I1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I8I1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023830"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027144.1">
        <property type="protein sequence ID" value="ENSGAGP00000023830.1"/>
        <property type="gene ID" value="ENSGAGG00000017438.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042605">
        <property type="term" value="F:peptide antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051006">
        <property type="entry name" value="TCR_variable_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19343">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19343:SF13">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-1279">T cell receptor</keyword>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="35"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="111" mass="12619" checksum="45832E005145DFCA" modified="2019-05-08" version="1">CVLSHQVSFRDCWGQVAWVESGQVSVEQSPEWRGVSEEQNGTMTCTYSCPITRLYWYRQDPGERPLFLPQENATEQRFMGQPLEDGKRSSLHITDSQLGDSGSYLCAVRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I943</accession>
    <name>A0A452I943_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I943"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027545.1">
        <property type="protein sequence ID" value="ENSGAGP00000024195.1"/>
        <property type="gene ID" value="ENSGAGG00000017691.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="159" mass="16699" checksum="5C886B76BE516433" modified="2019-05-08" version="1">AVGHFHCVGPSGAGPEGAGAPQAQPMLLDQFVLWAAVQTELEASALLREVQGLLRHTDGEGEVAAHLADDDGAADVVGLDLHLAGVRLFQDAQAAGSLLPPSGSCAITERQWQVLCGRLINLLLCAALVTLKDQRDLIYTATTQSNPAGRRSEAIHSCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBI9</accession>
    <name>A0A452IBI9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">CUB and zona pellucida like domains 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IBI9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025109"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028589.1">
        <property type="protein sequence ID" value="ENSGAGP00000025109.1"/>
        <property type="gene ID" value="ENSGAGG00000018366.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000005">
        <property type="entry name" value="Deleted in malignant brain tumors 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048290">
        <property type="entry name" value="ZP_chr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017977">
        <property type="entry name" value="ZP_dom_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002:SF38">
        <property type="entry name" value="CUB AND ZONA PELLUCIDA-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002">
        <property type="entry name" value="ENDOGLIN/TGF-BETA RECEPTOR TYPE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00023">
        <property type="entry name" value="ZPELLUCIDA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00682">
        <property type="entry name" value="ZP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="CUB" evidence="12">
        <location>
            <begin position="86"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="13">
        <location>
            <begin position="200"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="58626" checksum="DA93E99FA068FF64" modified="2019-05-08" version="1">VQHLQLQVPESLAKLSCTTRCKLKRDDGASCTRHSTLLAKGEKLSFNFSRFWHRLINHIQIYAKVYINDSYVFSFLTVYSSAKYTCGGILQYSSGTFQSPFYPGNYPNNADCVWEIEFTKCSCLFPRTEGGTCQYDYIEIYDGPLHTSPLLGKICHGSYLTYTSSSNLMTVRFHSDLSVTNRGFHAAYYTFPADQNTTLLCLPEYMHAVIRRSYLHSHGYDAWDVSLNDPYCRPIITSNYVIFNIPYNRCGTRREGNNNTIIYSNLIKAISSGYIIKRQKDLHLHINCKMLQNRWIEIMYVAEDVTEVNETQYGRYNVTISFYESPSFSRPVYDSPYYVDLNQNLFLQASIHSSDPNLVLFLDTCVASPDPNDFTTVTYDIIRHGCVRDPTYRVYYSPYASTLRFKFNAFKFINKHTSVYLKFKMVVCRVYDYSSRCYQGCTPRSKRDTSSYQEEVDVVVGPIQLRKDGTKNRNHTLFPLLSLTLMSHMYILLKSNICQNVTTMPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICE0</accession>
    <name>A0A452ICE0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000025471"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028989.1">
        <property type="protein sequence ID" value="ENSGAGP00000025471.1"/>
        <property type="gene ID" value="ENSGAGG00000018614.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000006">
        <property type="entry name" value="SEC14-like lipid binding 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.680">
        <property type="entry name" value="GOLD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051064">
        <property type="entry name" value="SEC14/CRAL-TRIO_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324">
        <property type="entry name" value="SEC14 RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324:SF39">
        <property type="entry name" value="SEC14-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="3">
        <location>
            <begin position="3"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="326"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="2">
        <location>
            <begin position="529"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="721" mass="82128" checksum="1F0BA3BDA57BF095" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICG2</accession>
    <name>A0A452ICG2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Formamidopyrimidine-DNA glycosylase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ICG2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025385"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028893.1">
        <property type="protein sequence ID" value="ENSGAGP00000025385.1"/>
        <property type="gene ID" value="ENSGAGG00000018561.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08967">
        <property type="entry name" value="MeNeil1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.190.10:FF:000003">
        <property type="entry name" value="endonuclease 8-like 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.10">
        <property type="entry name" value="MutM-like, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012319">
        <property type="entry name" value="FPG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035937">
        <property type="entry name" value="FPG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22993:SF27">
        <property type="entry name" value="ENDONUCLEASE 8-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22993">
        <property type="entry name" value="FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01149">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00898">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81624">
        <property type="entry name" value="N-terminal domain of MutM-like DNA repair proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51068">
        <property type="entry name" value="FPG_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formamidopyrimidine-DNA glycosylase catalytic" evidence="1">
        <location>
            <begin position="2"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="159" mass="17865" checksum="E110B1E009E4C967" modified="2019-05-08" version="1">MPEGPELHLASCYINTVCAGLIFSGKVEKSEVSKNPEVLFESNAYLISATSRGKEIKLTLTPLKEEKNTQNGLQQPMDLVFRFGMSGSFKLTSAAELPKHAHLRFYTKESPHRALCFVDFRRFGRWEVHGTWQLDRGPCVMLEYEKFRIGCCLGGGSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICQ7</accession>
    <name>A0A452ICQ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleolar GTP-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Associates with pre-60S ribosomal particles. Interacts with MINAS-60 (product of an alternative open reading frame of RBM10).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
            <location evidence="1 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. NOG subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ICQ7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025587"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029115.1">
        <property type="protein sequence ID" value="ENSGAGP00000025587.1"/>
        <property type="gene ID" value="ENSGAGG00000018689.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1190:FF:000001">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000496">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024926">
        <property type="entry name" value="NOG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012973">
        <property type="entry name" value="NOG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08155">
        <property type="entry name" value="NOGCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038919">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="620"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="11">
        <location>
            <begin position="158"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="483"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="531"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="445"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="533"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="552"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="639" mass="74797" checksum="D7CEC1CD4FEBF47F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IEB8</accession>
    <name>A0A452IEB8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cullin neddylation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IEB8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026244"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029841.1">
        <property type="protein sequence ID" value="ENSGAGP00000026244.1"/>
        <property type="gene ID" value="ENSGAGG00000019151.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22771:SF4">
        <property type="entry name" value="CULLIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22771">
        <property type="entry name" value="CULLIN AND GALACTOSE-BINDING DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin neddylation" evidence="1">
        <location>
            <begin position="126"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="251" mass="27786" checksum="E3FB24F47A8BF81D" modified="2019-05-08" version="1">MPRPPETSPHCPSPWAVAPRRLSWPPELSHHRHGPAPAYSLPVSFALQEVAVETLAQATGLSPVLLSHALKPLTGEAGILTQSLVAVLVPGVLRLNEAALARAAGQRLWLLPKQTYLNVEDDEGSNLERKRNIICCLIIQILKGEKELHIDNLVFRVIDACQKWESGPALKFLSFCCSSTDVLSCTLHLLSQGYLRRQEDNPQLLEYVSTEPTPSRSPPLQCPPQVTFQTVEIKTMPNTACAKRQTFSTFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IF85</accession>
    <name>A0A452IF85_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inhibitor of nuclear factor kappa-B kinase subunit alpha</fullName>
            <ecNumber evidence="3">2.7.11.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Nuclear factor NF-kappa-B inhibitor kinase alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[I-kappa-B protein] + ATP = O-phospho-L-seryl-[I-kappa-B protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19073"/>
            <dbReference type="Rhea" id="RHEA-COMP:13698"/>
            <dbReference type="Rhea" id="RHEA-COMP:13699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.10" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452IF85"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026541"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030168.1">
        <property type="protein sequence ID" value="ENSGAGP00000026541.1"/>
        <property type="gene ID" value="ENSGAGG00000019340.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008385">
        <property type="term" value="C:IkappaB kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008384">
        <property type="term" value="F:IkappaB kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033209">
        <property type="term" value="P:tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14039">
        <property type="entry name" value="STKc_IKK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17046">
        <property type="entry name" value="Ubl_IKKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.250:FF:000001">
        <property type="entry name" value="Inhibitor of nuclear factor kappa-B kinase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000061">
        <property type="entry name" value="Inhibitor of nuclear factor kappa-B kinase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000147">
        <property type="entry name" value="Inhibitor of nuclear factor kappa-B kinase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041185">
        <property type="entry name" value="IKBKB_SDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046375">
        <property type="entry name" value="IKBKB_SDD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051180">
        <property type="entry name" value="IKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022007">
        <property type="entry name" value="IKKbetaNEMObind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22969">
        <property type="entry name" value="IKB KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22969:SF13">
        <property type="entry name" value="INHIBITOR OF NUCLEAR FACTOR KAPPA-B KINASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18397">
        <property type="entry name" value="IKBKB_SDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12179">
        <property type="entry name" value="IKKbetaNEMObind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01239">
        <property type="entry name" value="IKKbetaNEMObind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="55"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="785" mass="88890" checksum="D78E553D7874F60C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452II06</accession>
    <name>A0A452II06_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tudor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000027475"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031208.1">
        <property type="protein sequence ID" value="ENSGAGP00000027475.1"/>
        <property type="gene ID" value="ENSGAGG00000019966.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000018">
        <property type="entry name" value="Serine/threonine-protein kinase 31"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050621">
        <property type="entry name" value="Tudor_domain_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF15">
        <property type="entry name" value="TUDOR DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="284"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="519"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="747"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="959"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="1286"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="1516"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1978" mass="223560" checksum="B30AF0FC159358CD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIG0</accession>
    <name>A0A452IIG0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Suppressor of fused homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUFU family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IIG0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027635"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031395.1">
        <property type="protein sequence ID" value="ENSGAGP00000027635.1"/>
        <property type="gene ID" value="ENSGAGG00000020053.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.230">
        <property type="entry name" value="Sufu, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020941">
        <property type="entry name" value="SUFU-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024314">
        <property type="entry name" value="SUFU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038489">
        <property type="entry name" value="SUFU_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037181">
        <property type="entry name" value="SUFU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007768">
        <property type="entry name" value="Suppressor_of_fused"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016591">
        <property type="entry name" value="Suppressor_of_fused_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10928">
        <property type="entry name" value="SUPPRESSOR OF FUSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10928:SF2">
        <property type="entry name" value="SUPPRESSOR OF FUSED HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05076">
        <property type="entry name" value="SUFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12470">
        <property type="entry name" value="SUFU_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011844">
        <property type="entry name" value="Suppressor_of_fused_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103359">
        <property type="entry name" value="Suppressor of Fused, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Suppressor of fused-like" evidence="3">
        <location>
            <begin position="47"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Suppressor of fused C-terminal" evidence="4">
        <location>
            <begin position="236"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Suppressor of fused C-terminal" evidence="4">
        <location>
            <begin position="443"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="306"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR011844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="496" mass="55400" checksum="AF0EC00C5C99A9E3" modified="2019-05-08" version="1">MEGARPGGAPPGAGPGSFPALFPPGLHGIYGECRRLYPEQPNPLLGGPDPLDYVSMYRNLGNPALNVPEHWHYVSFGLSDLYGDNRVHEFTGTDGPSGFGFELTFRLKRETGESAPPTWPAELMQGLARYVFQSENTFCSGDHVSWHSPLDNSESRIQHMLLTEDPQMQPVQTPFGIVNFLQIVGVCTEELHAAQQWNGQGILELLRTVPVAGGLWLITDMRRGETIFEIDPHLQERVDKGIETDGSNLSGVSAKCAWDDLSRPPEDDEDSRSICIGTQPRRLSGKDTEQIRETLRRGLEINSKPVLPPINAQRQNGLNYDRAPSRKDSLESESSAAIIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDTAITFVSTGVEGAFATEEHPYAAHGPWLQVRASFFPSSSFTSFLFSSFKKQSLVFPLQILLTEEFVERMLEDLEDLTSPEEFKLPKEYSWPEKKLKVSILPDAVFDNPLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJ64</accession>
    <name>A0A452IJ64_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">2-oxoglutarate dehydrogenase complex component E1</fullName>
            <ecNumber evidence="6">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Alpha-ketoglutarate dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:12189"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452IJ64"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027929"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031721.1">
        <property type="protein sequence ID" value="ENSGAGP00000027929.1"/>
        <property type="gene ID" value="ENSGAGG00000020280.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000007">
        <property type="entry name" value="2-oxoglutarate dehydrogenase e1 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000001">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF7">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="16" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="23">
        <location>
            <begin position="665"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00040429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00041946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00042799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1035" mass="117511" checksum="52A90A2BA8596304" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IK23</accession>
    <name>A0A452IK23_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Visual system homeobox 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Transcription factor VSX1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IK23"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028169"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032009.1">
        <property type="protein sequence ID" value="ENSGAGP00000028169.1"/>
        <property type="gene ID" value="ENSGAGG00000020469.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000065">
        <property type="entry name" value="Visual system homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023339">
        <property type="entry name" value="CVC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051775">
        <property type="entry name" value="Homeobox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24323">
        <property type="entry name" value="CEH-10 HOMEODOMAIN-CONTAINING HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24323:SF3">
        <property type="entry name" value="VISUAL SYSTEM HOMEOBOX 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51496">
        <property type="entry name" value="CVC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 13 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 13 14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 13 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0844">Vision</keyword>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="154"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="CVC" evidence="17">
        <location>
            <begin position="216"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="13">
        <location>
            <begin position="156"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="114"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="270"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="350" mass="37786" checksum="B0BB2A2E0E30CCE5" modified="2019-05-08" version="1">MTGREEPAEGRAKGKALVPSAASEKAARLGAPALRSKGFAITDLLGLEAELQPPAAPVAGAGCEGAGAGLGGVSLSGGSLPLGLGFLCGFAAPPPAGAPCLLPAHVPFLQPRQELHRAPGPPAGGRQENVSGKDEDSLSGDKGELKTPTSQIKRKKRRHRTVFTAHQLEELEKAFSDAHYPDVYAREMLAMKTELPEDRIQVWFQNRRAKWRKREKCWGRSSVMAEYGLYGAMVRHSIPLPESIINSAKSGLVGSCAPWLLGMHKKSLDVTRKPESEEKMTGSWRLEGPEEELKMKPADHQRSSGDKLSSMDNSEDTAIDLSSTAKQENRSALRQCLKGDLQTDGKDRDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKF4</accession>
    <name>A0A452IKF4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IKF4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028368"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032226.1">
        <property type="protein sequence ID" value="ENSGAGP00000028368.1"/>
        <property type="gene ID" value="ENSGAGG00000020585.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF72">
        <property type="entry name" value="NEUDESIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018990651" description="Cytochrome b5 heme-binding domain-containing protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="9">
        <location>
            <begin position="43"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="168" mass="18387" checksum="56D653E48DA1EAE8" modified="2019-05-08" version="1">MAGGPARLLALALGCLAAVAALAEQQQELRFKPAPAPTPVRLFTESELARYSGQEEGQPIYMAVKGVVFDVTSGKEFYGKGAPYNALVGKDSTRGVAKMSLDPEDLTHDTTGLTEEELKSLDDIFNDVYKAKYPIVGYTSQRILNEDGSPNQNFKPEDQPHFSIRDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IL24</accession>
    <name>A0A452IL24_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein phosphatase 1F</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Ca(2+)/calmodulin-dependent protein kinase phosphatase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Associates with FEM1B.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452IL24"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028604"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032488.1">
        <property type="protein sequence ID" value="ENSGAGP00000028604.1"/>
        <property type="gene ID" value="ENSGAGG00000020722.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000032">
        <property type="entry name" value="Protein phosphatase, Mg2+/Mn2+-dependent, 1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF233">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 18" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="20">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="292" mass="32219" checksum="EF55E40863A242A0" modified="2019-05-08" version="1">MEDRHVSLPEFNQLFGLSDDVDRAYFAVFDGHGGVDAANYAATHLHINVALHKEILKNPTEALKGSFQQTDEMFLFKAERERLRSGTTGVSALIVGNRLHIAWVGDSQVMLVQQGKAVTLMEPHKPEREDERMRIEALGGCVTYMDCWRVNGTLAVSRAIGDIYQKPYISGNADGNSFELTGSEDYLLLACDGFFDAIRPYDVVDLVLDHLKQSQGDGLKAAERLVAAAKENGSSDNITVLVVFLRDPRDLLADCLGDPKSLRADEGAEDSPFNFFSSEMNNQSDSHTTTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IM10</accession>
    <name>A0A452IM10_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Type I cytokine receptor cytokine-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IM10"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028908"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032838.1">
        <property type="protein sequence ID" value="ENSGAGP00000028908.1"/>
        <property type="gene ID" value="ENSGAGG00000020938.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000741">
        <property type="entry name" value="Interleukin 5 receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000818">
        <property type="entry name" value="Interleukin 5 receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003532">
        <property type="entry name" value="Short_hematopoietin_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015321">
        <property type="entry name" value="TypeI_recpt_CBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF46">
        <property type="entry name" value="INTERLEUKIN 5 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09240">
        <property type="entry name" value="IL6Ra-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01356">
        <property type="entry name" value="HEMATOPO_REC_S_F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019362760" description="Type I cytokine receptor cytokine-binding domain-containing protein" evidence="9">
        <location>
            <begin position="18"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Type I cytokine receptor cytokine-binding" evidence="10">
        <location>
            <begin position="130"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF09240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="428" mass="49417" checksum="3A4ACD8D4E1292DA" modified="2019-05-08" version="1">MAIVVSIFPVLLWRMMAFQANLLQAKGIQVLPPVNFTLTVSALAEVLLQWKPNPDQEKNHTVEYNVEIMTPEWEPYETKKTCSTRTAVLHNGFSARIRTLLLYNNSQIESNWVTAKLQAPPGAVETSVTNLSCVIYTAIDNTASLHCTWLAGKEAPEDTKYFLFYRYNSYTEECQEYSKDKWERNIGCRFSNTYIKTSETDEVIVIHINGSSKYTAIKPFEQLFNPQAIEKVNPPRNVTVFLKQNNLLIMWEMPASSFLEDCFEYELNIYNWKMDYRQILETMLNSFSLSIDDTCRYSIQIRANRQSWCSEGIWSDWTEPLHIGKNQPRTPVDSKAYTVLIALLVFICSVALLTAIICRKYHLWSRLFPPIPTPHNSLKNLFLNKSYQRTRNFTSETETEIGSCCEDLGSYVEDLSSDVLEDSCNMNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IM56</accession>
    <name>A0A452IM56_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IM56"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028932"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032865.1">
        <property type="protein sequence ID" value="ENSGAGP00000028932.1"/>
        <property type="gene ID" value="ENSGAGG00000020958.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01388">
        <property type="entry name" value="HMG-box_SoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000002">
        <property type="entry name" value="transcription factor Sox-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022097">
        <property type="entry name" value="SOX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF107">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12336">
        <property type="entry name" value="SOXp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="8">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="7">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="240" mass="26653" checksum="6CE8B8C75FDEC00F" modified="2019-05-08" version="1">MSKPTDHIKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSEAEKRPYIDEAKRLRAQHMKEHPDYKYRPRRKPKNLLKKDRYVFPLPYLGDTDPLKAAGLPVGATDALLSSPEKARAFLPPTSAPYSLLDPSQFSSSAIQKMTEVPHTLATSTLPYASTLGYQSGAFGSLSCPSQHTHTHPSPTNPGYVVPCNCTAWSASSLQPPVAYILFPGMTKTGIDPYSSAHATAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMA8</accession>
    <name>A0A452IMA8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Calsyntenin-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Postsynaptic adhesion molecule that binds to presynaptic neurexins to mediate both excitatory and inhibitory synapse formation. Promotes synapse development by acting as a cell adhesion molecule at the postsynaptic membrane, which associates with both neurexin-alpha and neurexin-beta proteins at the presynaptic membrane. Regulates the balance between excitatory and inhibitory synapses by inhibiting formation of excitatory parallel-fiber synapses and promoting formation of inhibitory synapses in the same neuron. May also be involved in ascorbate (vitamin C) uptake via its interaction with SLC23A2/SVCT2. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Interacts (via cadherin domains) with both alpha and beta isoforms of neurexins (NRXN1, NRXN2 and NRXN3). Directly interacts with APBA2. Forms a tripartite complex with APBA2 and APP. Interacts with low affinity with KLC1. Interacts with SLC23A2/SVCT2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Postsynaptic cell membrane</location>
            <topology evidence="18">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the calsyntenin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IMA8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028971"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032908.1">
        <property type="protein sequence ID" value="ENSGAGP00000028971.1"/>
        <property type="gene ID" value="ENSGAGG00000020983.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050806">
        <property type="term" value="P:positive regulation of synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000025">
        <property type="entry name" value="Calsyntenin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000069">
        <property type="entry name" value="Calsyntenin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000062">
        <property type="entry name" value="Calsyntenin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045588">
        <property type="entry name" value="CLSTN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14139">
        <property type="entry name" value="CALSYNTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14139:SF5">
        <property type="entry name" value="CALSYNTENIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19699">
        <property type="entry name" value="CLSTN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 23" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 26" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 25" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019107109" description="Calsyntenin-3" evidence="26">
        <location>
            <begin position="23"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="847"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="27">
        <location>
            <begin position="32"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="27">
        <location>
            <begin position="149"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="921"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="923"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="935"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="959"/>
            <end position="972"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00035015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00046243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00046399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="972" mass="107825" checksum="45C2794CA4012CBD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMJ7</accession>
    <name>A0A452IMJ7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the RecA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IMJ7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029307"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033286.1">
        <property type="protein sequence ID" value="ENSGAGP00000029307.1"/>
        <property type="gene ID" value="ENSGAGG00000021203.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF30">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN DMC1_LIM15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 1" evidence="5">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="6">
        <location>
            <begin position="107"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="170" mass="19146" checksum="AEA7EA1D824D55B3" modified="2019-05-08" version="1">MRKLESSSYWYEPFGNFTLLHQLNAFRIIFSFAPSFSFTTFILFCIFTMQIIDSIMALFRVDFSGRGELAERQQKLAQMLSRLQKISEEYNVAVFVTNQMTADPGATMTFQADPKKPIGGHILAHASTTRISLRKGRGELRIAKIYDSPEMPENEATFAITAGGIGDAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IP75</accession>
    <name>A0A452IP75_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Exostosin like glycosyltransferase 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IP75"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029904"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033957.1">
        <property type="protein sequence ID" value="ENSGAGP00000029904.1"/>
        <property type="gene ID" value="ENSGAGG00000021583.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015338">
        <property type="entry name" value="GT64_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261">
        <property type="entry name" value="ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261:SF3">
        <property type="entry name" value="EXOSTOSIN GLYCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09258">
        <property type="entry name" value="Glyco_transf_64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Exostosin GT47" evidence="13">
        <location>
            <begin position="102"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase 64" evidence="14">
        <location>
            <begin position="481"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="741" mass="85432" checksum="00BEA588FF31B3B3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IR85</accession>
    <name>A0A452IR85_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Katanin p60 ATPase-containing subunit A-like 2</fullName>
            <shortName evidence="8">Katanin p60 subunit A-like 2</shortName>
            <ecNumber evidence="8">5.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">p60 katanin-like 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KATNAL2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Severs microtubules in vitro in an ATP-dependent manner. This activity may promote rapid reorganization of cellular microtubule arrays.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers.</text>
            <dbReference type="EC" id="5.6.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
            <location evidence="1 8">Cytoskeleton</location>
            <location evidence="1 8">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
            <location evidence="8">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
            <location evidence="8">Cytoskeleton</location>
            <location evidence="8">Spindle</location>
        </subcellularLocation>
        <text evidence="8">Localizes within the cytoplasm, partially overlapping with microtubules in interphase and to the mitotic spindle and spindle poles during mitosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily. A-like 2 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.1" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A452IR85"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030374"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034471.1">
        <property type="protein sequence ID" value="ENSGAGP00000030374.1"/>
        <property type="gene ID" value="ENSGAGG00000021882.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008568">
        <property type="term" value="F:microtubule severing ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051013">
        <property type="term" value="P:microtubule severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19509">
        <property type="entry name" value="RecA-like_VPS4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000048">
        <property type="entry name" value="Katanin p60 ATPase-containing subunit A-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000434">
        <property type="entry name" value="Katanin p60 ATPase-containing subunit A-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03025">
        <property type="entry name" value="Katanin_p60_AL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027497">
        <property type="entry name" value="Katanin_p60_AL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF78">
        <property type="entry name" value="KATANIN P60 ATPASE-CONTAINING SUBUNIT A-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="116"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="124"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="368" mass="41199" checksum="56947E0CEB3122A7" modified="2019-05-08" version="1">MELSYQTLKTTHRAKEACQIIDFRGMIQDAIKGASNEVASNSPDPSERLLKPLSAFIGMSGEMRELATVISRDIYLHNPNIKWDDIIGLDAAKQLVKEAVVYPIRYPQLFTGILSPWKGLLLYGPPGTGKTLLAKAVATECNTTFFNISASTIVSKWRGDSEKLVRVLFELARYHAPSTIFLDELESVMSQRGNHPGGEHEGSRRMKTELLVQMDGLARSDDLVFVLAASNLPWELDYAMLRRLEKRILVDLPNKEARQAMIQHWLPPVSNSGGVELRTELEYSLLGQETEGYSGSDVKLVCKEAAMRPVRKIFNALENHQPGSSNLPELRLDTVTTADFLDVIAHTKPSAKNLSQKYIAWQRDFESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IU28</accession>
    <name>A0A452IU28_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">A disintegrin and metalloproteinase with thrombospondin motifs 18</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IU28"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031544"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035775.1">
        <property type="protein sequence ID" value="ENSGAGP00000031544.1"/>
        <property type="gene ID" value="ENSGAGG00000022650.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090331">
        <property type="term" value="P:negative regulation of platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04273">
        <property type="entry name" value="ZnMc_ADAMTS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000006">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.60:FF:000002">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000066">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000009">
        <property type="entry name" value="ADAMTS-like protein 3 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF167">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08686">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50900">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="10 16 18" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="20">
        <location>
            <begin position="337"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="PLAC" evidence="21">
        <location>
            <begin position="1231"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="active site" evidence="15 18">
        <location>
            <position position="481"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 18">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="537"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="540"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="540"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="413"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="439"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="458"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="497"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="565"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="576"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="585"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="610"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="645"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="649"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="660"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1268" mass="141670" checksum="C49FB289286AC837" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IU98</accession>
    <name>A0A452IU98_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Carboxypeptidase B2</fullName>
            <ecNumber evidence="20">3.4.17.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Carboxypeptidase U</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Thrombin-activable fibrinolysis inhibitor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Release of C-terminal Arg and Lys from a polypeptide.</text>
            <dbReference type="EC" id="3.4.17.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 24">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.20" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A452IU98"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031467"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035690.1">
        <property type="protein sequence ID" value="ENSGAGP00000031467.1"/>
        <property type="gene ID" value="ENSGAGG00000022598.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042730">
        <property type="term" value="P:fibrinolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06246">
        <property type="entry name" value="M14_CPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.340:FF:000003">
        <property type="entry name" value="Carboxypeptidase B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000084">
        <property type="entry name" value="Carboxypeptidase B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033849">
        <property type="entry name" value="CPB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF17">
        <property type="entry name" value="CARBOXYPEPTIDASE B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0280">Fibrinolysis</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase M14" evidence="25">
        <location>
            <begin position="104"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="24">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00053927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00066563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00070087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00075534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00078332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="384" mass="44375" checksum="673442BDE85C5E8F" modified="2019-05-08" version="1">LKQANIPTNELPSTLPKDDRDVVICQFKMSFRVVLWQPVSAENIMKNKEVHFYVNVSDINDVKAELNRSTIPYKVLMDDVQGVIKKQTVNDTVSPRSSASYYEQYHSLNEIYSWMEEITEMNADLLQKIYIGSSFEKRPLYILKVQTPKSAIWIDCGIHAREWIAPAFCLWFIGHTTHFREKDRTMTRLLQHLDFYIMPVMNVDGYEYTWTTVRLIYWTDLKAYFSKCKGASHNDCHEIYCGPYPESEPEVKAVAHFLQKHKDHIKAYITIHSYSQMVLFPYSYTTNKSKDHDELLSVANKVVLAIRKTTQKMYESGPGAQTIYLAPGGSDDWAYDLGIKYSFTFELRDRGTHGFLLPPNLIKPTCIEALSGIKTIALHVINNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IVF1</accession>
    <name>A0A452IVF1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Iron-binding zinc finger CDGSH type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IVF1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032054"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036342.1">
        <property type="protein sequence ID" value="ENSGAGP00000032054.1"/>
        <property type="gene ID" value="ENSGAGG00000022976.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.90">
        <property type="entry name" value="CDGSH iron-sulfur domain, mitoNEET-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052950">
        <property type="entry name" value="CISD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018967">
        <property type="entry name" value="FeS-contain_CDGSH-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042216">
        <property type="entry name" value="MitoNEET_CISD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46491">
        <property type="entry name" value="CDGSH IRON SULFUR DOMAIN PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46491:SF3">
        <property type="entry name" value="CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09360">
        <property type="entry name" value="zf-CDGSH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00704">
        <property type="entry name" value="ZnF_CDGSH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019378246" description="Iron-binding zinc finger CDGSH type domain-containing protein" evidence="6">
        <location>
            <begin position="17"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Iron-binding zinc finger CDGSH type" evidence="7">
        <location>
            <begin position="44"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Iron-binding zinc finger CDGSH type" evidence="7">
        <location>
            <begin position="82"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="127" mass="13692" checksum="35F92708E3AFF440" modified="2019-05-08" version="1">MPALQRLAALVTLVSGACTKAAGRWVRSSLGTSSSPSRPAIAAKHPYQVDLKAGKRYAWCACGHSKKQPFCDGSHKRATPDLSPLKFQLEEAKSAWLCGCKHTKSPPYCDGTHKGEFIQKAELPGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWL7</accession>
    <name>A0A452IWL7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IWL7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032304"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036622.1">
        <property type="protein sequence ID" value="ENSGAGP00000032304.1"/>
        <property type="gene ID" value="ENSGAGG00000023120.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08741">
        <property type="entry name" value="RGS_RGS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.196.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046995">
        <property type="entry name" value="RGS10/12/14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037879">
        <property type="entry name" value="RGS10_RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024066">
        <property type="entry name" value="RGS_subdom1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45945">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING LOCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45945:SF3">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING LOCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="42"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="182" mass="21305" checksum="6D1B59C0C8B325BD" modified="2019-05-08" version="1">MFTRAVSRLSRKRPPSEINDSDGKPGGSSQQTLKGTSRWTTSLENLLEDPEGVKRFREFLKKEFSEENVLFWLACEEFKKTQDKKQMQEKAKEIYMTFLSSKASCQVNVEGQSRLNETILEEPHPLMFQKLQDQIFNLMKYDSYSRFLKSDIFLKHKRAEEQEENSSEAQSVAKRASRIYNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IXE6</accession>
    <name>A0A452IXE6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Globin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the globin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IXE6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032723"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037087.1">
        <property type="protein sequence ID" value="ENSGAGP00000032723.1"/>
        <property type="gene ID" value="ENSGAGG00000023386.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072562">
        <property type="term" value="C:blood microparticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031838">
        <property type="term" value="C:haptoglobin-hemoglobin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005833">
        <property type="term" value="C:hemoglobin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031720">
        <property type="term" value="F:haptoglobin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043177">
        <property type="term" value="F:organic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08927">
        <property type="entry name" value="Hb-alpha-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.490.10:FF:000002">
        <property type="entry name" value="Hemoglobin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002338">
        <property type="entry name" value="Hemoglobin_a-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050056">
        <property type="entry name" value="Hemoglobin_oxygen_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002339">
        <property type="entry name" value="Hemoglobin_pi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11442">
        <property type="entry name" value="HEMOGLOBIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00612">
        <property type="entry name" value="ALPHAHAEM"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00815">
        <property type="entry name" value="PIHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Globin" evidence="8">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="141" mass="15817" checksum="3621B811D3B8FE15" modified="2019-05-08" version="1">MLTEDEKHLIEHVWEKVLEHQEDFGAEALERLFTVYPSTKTYFPHFDLHHDSAQIRAHGKKVLSALGEAVNHIDDIPAALSKLSDLHAQTLRVDPVNFKLLSLCVVVVVGRNNPSVLTPEVHVSLDKFLSAVSTALTAKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYK4</accession>
    <name>A0A452IYK4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYK4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033176"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037600.1">
        <property type="protein sequence ID" value="ENSGAGP00000033176.1"/>
        <property type="gene ID" value="ENSGAGG00000023666.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990080">
        <property type="term" value="F:2-phenylethylamine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001662">
        <property type="term" value="P:behavioral fear response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15312">
        <property type="entry name" value="7tmA_TAAR2_3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000030">
        <property type="entry name" value="trace amine-associated receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009132">
        <property type="entry name" value="TAAR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF220">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01830">
        <property type="entry name" value="TRACEAMINER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="43"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="340" mass="38926" checksum="C0461B82C052CB85" modified="2019-05-08" version="1">MLPIPWYCFVSVNNSCPRNVRSTSSLWAMYLFMVGAIVLTMGENMLVIISITHFKQLHSPTNFLICSMATTDFLLSFMVMLYSMIRSIESFWYFGDLFCKIHTCCDIMLCTTSIFHLCFISVDRYYAVCDPLHYVTKITIPVIVLFLLISWSVPFLLAFGLVFSELNIEGIEEHVTSINCCGFCAFILNKLWEVMASLIAFFFPDTVMVGVYVHIFTVARKHARQIAKIPSAIKCISAMKNKLSTKKENKATKTLSIVMGVFVFCWLPFFILTIADPFINFSTPEDLYNSFLWLGYFNSTCNPIIYGLFYSWFCKPFKMIVTGTIFRPDTSTLTLFPLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYW2</accession>
    <name>A0A452IYW2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000033297"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037728.1">
        <property type="protein sequence ID" value="ENSGAGP00000033297.1"/>
        <property type="gene ID" value="ENSGAGG00000023735.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024845">
        <property type="entry name" value="NHS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23039:SF5">
        <property type="entry name" value="ACTIN REMODELING REGULATOR NHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23039">
        <property type="entry name" value="NANCE-HORAN SYNDROME PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15273">
        <property type="entry name" value="NHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="655"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="800"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="838"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1145"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1187"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1276"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1359"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="655"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="679"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="800"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="840"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1030"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1161"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1292"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1323"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1389"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1422"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1473"/>
            <end position="1484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1484" mass="161964" checksum="B674102BE1E37632" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYX1</accession>
    <name>A0A452IYX1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM167 (SEC) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYX1"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037742.1">
        <property type="protein sequence ID" value="ENSGAGP00000033307.1"/>
        <property type="gene ID" value="ENSGAGG00000023743.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024280">
        <property type="entry name" value="FAM167"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051771">
        <property type="entry name" value="FAM167_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32289">
        <property type="entry name" value="PROTEIN FAM167A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32289:SF4">
        <property type="entry name" value="PROTEIN FAM167B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11652">
        <property type="entry name" value="FAM167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="192" mass="21987" checksum="F2D17D80FDFCF698" modified="2019-05-08" version="1">MSFSHLKFKELGEEESNSDKENLDSVKALTAKLKLQTRRPSYLEWKARVQSQSWRNGVKEGAISSLKEEQGREVFLEGTQADTPSRSICGFATMDDALEWLRKELQEMQAVDHQLARQLMRLRGQIHQLKVEQVCHQHKEMLDNATFGLEGCEEDTDLLCNIPPKAAFLLSTPLKHIGVTRMNINSRRFSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZ56</accession>
    <name>A0A452IZ56_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tyrosine-protein phosphatase non-receptor type</fullName>
            <ecNumber evidence="8">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
            <location evidence="1 8">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A452IZ56"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033355"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037795.1">
        <property type="protein sequence ID" value="ENSGAGP00000033355.1"/>
        <property type="gene ID" value="ENSGAGG00000023738.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13188">
        <property type="entry name" value="FERM_C_PTPN14_PTPN21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000014">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000149">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000030">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014392">
        <property type="entry name" value="PTP_non-rcpt_14/21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041782">
        <property type="entry name" value="PTPN14/21_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706:SF3">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000934">
        <property type="entry name" value="Tyr-Ptase_nr14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="14">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="863"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="1045"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="351"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="9">
        <location>
            <position position="1075"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000934-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1141" mass="130828" checksum="DB56459F591306D2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZC6</accession>
    <name>A0A452IZC6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452IZC6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033434"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037877.1">
        <property type="protein sequence ID" value="ENSGAGP00000033434.1"/>
        <property type="gene ID" value="ENSGAGG00000023824.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000343">
        <property type="entry name" value="Ras family gtpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="211" mass="23958" checksum="6DB6651F83FE62BA" modified="2019-05-08" version="1">MELENEMISSSSNSSAGSREYKVVMLGAGGVGKSAMTMQFISHRFPDYHDPTIEDAYKTQVRIDDEPAYLDILDTAGQAEFTAMRDQYMRGGEGFIICYSITDRQSFQEAAEFKELIYRVRHTYDIPLVLVGNKIDLEEFRQVSEVSSCSFSSNTSTLCKNEGINTIIEAQLKCIPQIKKNSKRTQKEPLWLNNHVKEAVGDKKASFKKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0T1</accession>
    <name>A0A452J0T1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nociceptin receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Kappa-type 3 opioid receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Orphanin FQ receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">G-protein coupled opioid receptor that functions as a receptor for the endogenous neuropeptide nociceptin. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling via G proteins mediates inhibition of adenylate cyclase activity and calcium channel activity. Arrestins modulate signaling via G proteins and mediate the activation of alternative signaling pathways that lead to the activation of MAP kinases. Plays a role in modulating nociception and the perception of pain. Plays a role in the regulation of locomotor activity by the neuropeptide nociceptin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J0T1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033921"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038415.1">
        <property type="protein sequence ID" value="ENSGAGP00000033921.1"/>
        <property type="gene ID" value="ENSGAGG00000024122.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001626">
        <property type="term" value="F:nociceptin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019233">
        <property type="term" value="P:sensory perception of pain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15092">
        <property type="entry name" value="7tmA_NOFQ_opioid_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000014">
        <property type="entry name" value="Kappa-type opioid receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001418">
        <property type="entry name" value="Opioid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001420">
        <property type="entry name" value="X_opioid_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF11">
        <property type="entry name" value="NOCICEPTIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00384">
        <property type="entry name" value="OPIOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00547">
        <property type="entry name" value="XOPIOIDR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0085">Behavior</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 20" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="12 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 20" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 20 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="22">
        <location>
            <begin position="65"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="367" mass="41380" checksum="001258713EBB07DB" modified="2019-05-08" version="1">MDPLFPAHILEDPDLRKLLNDSSMLNLSFPSNWFNNGTEDSFLPLSIKITIVVVYSIVCIVGLVGNCSVMYVIVRFTKMKTATNIYIFNLALADTLCLITLPFQGTDTFLGFWPFGNILCKIAISIDYYNMFTSTFTLTMMSVDRYIAICHPIKALDIRTPHKAKVVNICIWALASVFGIPAMVMGSAENENNEIDCLIKLPKPVDYWDPVFGICVFLFSFMIPVLIITICYSLMIRRLKNVRVLSGSKEKDRNLRRITRMVLVVVAVFIICWTPIQIFVLVQCLGAKAESELELAISCFCTALGYANSSLNPVLYAFLDENFKTCFRKFCFPTAFRTELQMSNRMCSIAKDVVYACKNSEGTNNPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1S6</accession>
    <name>A0A452J1S6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J1S6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034341"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038883.1">
        <property type="protein sequence ID" value="ENSGAGP00000034341.1"/>
        <property type="gene ID" value="ENSGAGG00000024400.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015269">
        <property type="term" value="F:calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005513">
        <property type="term" value="P:detection of calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003930">
        <property type="entry name" value="K_chnl_Ca-activ_BK_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10258">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10258:SF1">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03185">
        <property type="entry name" value="CaKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01450">
        <property type="entry name" value="BKCHANNELB"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="215" mass="24727" checksum="4C881047521C1C03" modified="2019-05-08" version="1">MLGKKLVTSQKRGETRALCLGLGMVACSVMMYFFIGITIMPSYTNSVWTKETTCKLLKTNIKDKVQCSFNNGADDENIFQYPCLEVLVDLNFSGQEVMLYHNEETQERNPKCSYIPRNLKNDKEVKMLIETAAENFRKHQTFPCYYDPERRKTSVILIRLYPPKGLFFAFLWPSLMLTGGVLIIIMVKVSQYVSVLSACERIQLINEHETICFAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J341</accession>
    <name>A0A452J341_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHHC U11-48K-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J341"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034847"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039455.1">
        <property type="protein sequence ID" value="ENSGAGP00000034847.1"/>
        <property type="gene ID" value="ENSGAGG00000024765.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051591">
        <property type="entry name" value="UPF0224_FAM112_RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21402:SF5">
        <property type="entry name" value="GAMETOCYTE SPECIFIC FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21402">
        <property type="entry name" value="GAMETOCYTE SPECIFIC FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="CHHC U11-48K-type" evidence="4">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="108" mass="12172" checksum="122ED6C69D8976BC" modified="2019-05-08" version="1">MDPERLIPCPYDKNHQIRASRFPYHLVKCGENNKKRAKELATCPYNARHKVPKQELQLHISTCTDNQEPELFSGKLRGGGEVQGVHACFNTCLLLLTDLTCPGLGRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J524</accession>
    <name>A0A452J524_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(+) ADP-ribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.30</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.30" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A452J524"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035459"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040153.1">
        <property type="protein sequence ID" value="ENSGAGP00000035459.1"/>
        <property type="gene ID" value="ENSGAGG00000025209.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000025">
        <property type="entry name" value="Ankyrin repeat and sterile alpha motif domain-containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="65"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="98"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="177"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="211"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="312"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="346"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="10">
        <location>
            <begin position="811"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="410"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="469"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="581"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="715"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="416"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="480"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="612"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="652"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="719"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="749"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="937" mass="101964" checksum="1A1357291D43D238" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J576</accession>
    <name>A0A452J576_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ribosomal protein S6 kinase</fullName>
            <ecNumber evidence="12">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452J576"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035612"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040327.1">
        <property type="protein sequence ID" value="ENSGAGP00000035612.1"/>
        <property type="gene ID" value="ENSGAGG00000024978.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004711">
        <property type="term" value="F:ribosomal protein S6 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14178">
        <property type="entry name" value="STKc_RSK3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05582">
        <property type="entry name" value="STKc_RSK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000010">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000041">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000013">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000121">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016239">
        <property type="entry name" value="Ribosomal_S6_kinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042766">
        <property type="entry name" value="RSK3_STKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041906">
        <property type="entry name" value="RSK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000606">
        <property type="entry name" value="Ribsml_S6_kin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="132"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="18">
        <location>
            <begin position="392"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="488"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="605"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="138"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="494"/>
            <end position="502"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="517"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000606-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000606-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="806" mass="90479" checksum="E3C500792DD000FF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7G5</accession>
    <name>A0A452J7G5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Lysozyme g</fullName>
            <ecNumber evidence="3 10">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 23 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="3 10"/>
    <dbReference type="AlphaFoldDB" id="A0A452J7G5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036446"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041279.1">
        <property type="protein sequence ID" value="ENSGAGP00000036446.1"/>
        <property type="gene ID" value="ENSGAGG00000025847.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01021">
        <property type="entry name" value="GEWL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000026">
        <property type="entry name" value="Lysozyme g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002152">
        <property type="entry name" value="Glyco_hydro_23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31698">
        <property type="entry name" value="LYSOZYME G FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31698:SF8">
        <property type="entry name" value="LYSOZYME G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001065">
        <property type="entry name" value="Lysozyme_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00749">
        <property type="entry name" value="LYSOZYMEG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="6" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019568325" description="Lysozyme g" evidence="12">
        <location>
            <begin position="19"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Transglycosylase SLT" evidence="13">
        <location>
            <begin position="76"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001065-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="209" mass="22784" checksum="0ED7792744358D84" modified="2019-05-08" version="1">CLRRTVPMLLTLMILGLAALIGESGTGCYGNINKVDTTGASCRTAKAEHLSYCGVRASTTIAAKDLRAMNNYKTIIKNAGRKRCVDPAVIAGIISRESHAGKALKGGWGDRGNGFGLMQVDKRYHRLVGRWNSEAHVLQGTGILVSMIKGIQKKFPRWTKDQQLKGGISAYNAGLRNVQTYNKMDIGTTGNDYANDVVARAKFFKRNGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GEY2</accession>
    <name>A0A452GEY2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-crystallin B3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Beta-B3 crystallin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homo/heterodimer, or complexes of higher-order. The structure of beta-crystallin oligomers seems to be stabilized through interactions between the N-terminal arms.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GEY2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000103"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000119.1">
        <property type="protein sequence ID" value="ENSGAGP00000000103.1"/>
        <property type="gene ID" value="ENSGAGG00000000077.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000005">
        <property type="entry name" value="Crystallin, beta B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000002">
        <property type="entry name" value="Crystallin, beta B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF13">
        <property type="entry name" value="BETA-CRYSTALLIN B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="64"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="156"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="211" mass="24420" checksum="474C045CBA2493DF" modified="2019-05-08" version="1">MTEPQSPPEPMAAGEHLGERGGNYKITIFELENFQGKKRELTEESPNVTEEALEKVGSIQVESGPWLGFERQAFCGEQFVLEKGAYPRWDSWSNSQSSDSLMSVRPLLIDSHDHKIHLFENAGYNGRKMEIVDDDVPSLWAHGFQDRVASIKALNGTWVGYEYPSYRGRQYVFERGEYQHWNEWDASQPQIQSVRRVRDQQWHKHGCFETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFI6</accession>
    <name>A0A452GFI6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Long-chain-fatty-acid--CoA ligase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:54537"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tetracosanoate + ATP + CoA = tetracosanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33639"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:33640"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GFI6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000326"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000369.1">
        <property type="protein sequence ID" value="ENSGAGP00000000326.1"/>
        <property type="gene ID" value="ENSGAGG00000000246.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000005">
        <property type="entry name" value="Solute carrier family 27 member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF12">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="13">
        <location>
            <begin position="103"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="509"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="526"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="541" mass="58962" checksum="BEB72C7F049C69C2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GG36</accession>
    <name>A0A452GG36_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the gamma-tubulin ring complex (gTuRC) consisting of TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 and gamma-tubulin TUBG1 or TUBG2. TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 assemble in a 5:5:2:1:1 stoichiometry; each is associated with a gamma-tubulin, thereby arranging 14 gamma-tubulins in a helical manner. Gamma-tubulin at the first position is blocked by TUBGCP3 at the last position, allowing 13 protafilaments to grow into a microtubule. The gTuRC (via TUBGCP3 and TUBGCP6) interacts with ACTB and MZT1; the interactions form a luminal bridge that stabilizes the initial structure during complex assembly. The gTuRC (via TUBGCP2) interacts with MZT2A/MZT2B and CDK5RAP2 (via CM1 motif); the interactions play a role in gTuRC activation. Interacts with NINL. Interacts with ATF5; the ATF5:PCNT:polyglutamylated tubulin (PGT) tripartite unites the mother centriole and the pericentriolar material (PCM) in the centrosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GG36"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000532"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000592.1">
        <property type="protein sequence ID" value="ENSGAGP00000000532.1"/>
        <property type="gene ID" value="ENSGAGG00000000438.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1900:FF:000001">
        <property type="entry name" value="Gamma-tubulin complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF27">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="10">
        <location>
            <begin position="2"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="9">
        <location>
            <begin position="350"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="764" mass="87024" checksum="C5FCD72622914A75" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGQ7</accession>
    <name>A0A452GGQ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-alanine(in) + Na(+)(in) = L-alanine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29283"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-asparagine(in) + Na(+)(in) = L-asparagine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:71383"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-cysteine(in) + Na(+)(in) = L-cysteine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:68232"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-glutamine(in) + Na(+)(in) = L-glutamine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:68236"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-isoleucine(in) + Na(+)(in) = L-isoleucine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29275"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-leucine(in) + Na(+)(in) = L-leucine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29263"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-methionine(in) + Na(+)(in) = L-methionine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:68240"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-phenylalanine(in) + Na(+)(in) = L-phenylalanine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:68244"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-pipecolate(in) + Na(+)(in) = L-pipecolate(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:71387"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:61185"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-serine(in) + Na(+)(in) = L-serine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29575"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonine(in) + Na(+)(in) = L-threonine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69999"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-valine(in) + Na(+)(in) = L-valine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29267"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A15 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GGQ7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000749"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000845.1">
        <property type="protein sequence ID" value="ENSGAGP00000000749.1"/>
        <property type="gene ID" value="ENSGAGG00000000638.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005298">
        <property type="term" value="F:proline:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015820">
        <property type="term" value="P:L-leucine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11522">
        <property type="entry name" value="SLC6sbd_SBAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042934">
        <property type="entry name" value="B(0)AT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002438">
        <property type="entry name" value="Neutral_aa_SLC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF101">
        <property type="entry name" value="SODIUM-DEPENDENT NEUTRAL AMINO ACID TRANSPORTER B(0)AT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01206">
        <property type="entry name" value="ORPHTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="27" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 27" id="KW-0915">Sodium</keyword>
    <keyword evidence="13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 28" id="KW-0769">Symport</keyword>
    <keyword evidence="5 28 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 28" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="458"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="533"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="571"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="614"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00034271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00034276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00034279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00034282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00034289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="729" mass="81757" checksum="A43B8176A24CBD52" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GID9</accession>
    <name>A0A452GID9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452GID9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001411"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001596.1">
        <property type="protein sequence ID" value="ENSGAGP00000001411.1"/>
        <property type="gene ID" value="ENSGAGG00000001123.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="6"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="171"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="420" mass="47193" checksum="C26ABB724746FFAF" modified="2019-05-08" version="1">FTNRKEKLSFQVSEDCLFLNVYTPASSDKDMLPVMVWIHGGAFVIGAASTYDGSALSAFENVVVVTVQYRLGILGYFSSGDEHARGNWGYLDQVAALQWIQENIEHFGGDPGSVTIFGESAGGFSVSALVLSPLAKGLFHKAISESGVTIKIFFTAHPEAQANVGIFFLLQSLLISTVLDGVFFPKSPEELLAEKAINAVPYIIGVNTHEFGWLIPNMFKYPAFTEGLDKETVTSLLLASEFFTHIPAEFTYLLTNEYLSNIEEPTQLRDRLFELMGDAVFVVPVIQRARYHRDSGNPVYIYHFHHRSSSYEGVRPDFLKADHGDEIGYVFGKPFLAGDATEEEGKLSKTIMKYWANFARKGNPNGEGLMTWPVYNVDEQYLIIDIKQKAAKKLKENRVEFWTKTLPKKMAEEKRKYKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJ54</accession>
    <name>A0A452GJ54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GJ54"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001642"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001865.1">
        <property type="protein sequence ID" value="ENSGAGP00000001642.1"/>
        <property type="gene ID" value="ENSGAGG00000001312.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049218">
        <property type="entry name" value="DUF5575_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049217">
        <property type="entry name" value="DUF5575_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040854">
        <property type="entry name" value="ZSWIM9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048315">
        <property type="entry name" value="ZSWIM9_RNaseH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47086">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47086:SF1">
        <property type="entry name" value="ZINC FINGER SWIM-TYPE CONTAINING 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17738">
        <property type="entry name" value="DUF5575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20784">
        <property type="entry name" value="DUF5575_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20783">
        <property type="entry name" value="DUF5575_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="477"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="529" mass="60072" checksum="84964ABBCE94ACA6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJE1</accession>
    <name>A0A452GJE1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coiled-coil domain containing 87</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GJE1"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001948.1">
        <property type="protein sequence ID" value="ENSGAGP00000001713.1"/>
        <property type="gene ID" value="ENSGAGG00000001374.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037383">
        <property type="entry name" value="CCDC87"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16078">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16078:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="58025" checksum="E4BA5B2BDFDCFCA0" modified="2019-05-08" version="1">MGILTLNAIKLGGTHFPEDTELPPIIGALTRRRANVTKLQQLQETLRCLQEEEMAEQQLRYMVVFAPPTHPQAATVNLQIHHGMVAKAADLQVSDRVLVDSVAIQRYGPLYNDLLGEIDTATVSYLDANLSAGEEIREIYKELIKIIPTEYLRFDQGPLIEPAAMNVDLSNSLASSTLTKRKSEQVINTELNKLLPAGPFSTQKVITPVQSSLPLMIAREQKTTWYQWWKSALNADDYLKYVSTKESDYLHVIFHLYNEGEEPLFIDEAQKKQLEETQREVKRKVAEVRSKREKYMPGMWNTNTIMLGGLGTFEDQDRKPGDLRLLQKQLERLWEVLHFPDRERLDMAIKYSSNQSYAQLPAMLKAWEKAAKSIQERELLLLELEKFEQTASDPNRFFERSLESFATRTWESRTRGRLYAAIAQCDTDLYIILHQIKERFHDTVTFKGRPYLEKMRWDKVEMLYWLQQERRAGALAKETGREARLWKDPNHPALGHLSTTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJM5</accession>
    <name>A0A452GJM5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">L-Fucosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a ganglioside GM1 + GDP-beta-L-fucose = a ganglioside Fuc-GM1 + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:82639"/>
            <dbReference type="ChEBI" id="CHEBI:90189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:48293"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Golgi apparatus</location>
            <location evidence="1 7">Golgi stack membrane</location>
            <topology evidence="1 7">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glycosyltransferase 11 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452GJM5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001892"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002151.1">
        <property type="protein sequence ID" value="ENSGAGP00000001892.1"/>
        <property type="gene ID" value="ENSGAGG00000001518.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008107">
        <property type="term" value="F:galactoside 2-alpha-L-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11301">
        <property type="entry name" value="Fut1_Fut2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002516">
        <property type="entry name" value="Glyco_trans_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11927">
        <property type="entry name" value="GALACTOSIDE 2-L-FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11927:SF9">
        <property type="entry name" value="L-FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01531">
        <property type="entry name" value="Glyco_transf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="345" mass="39174" checksum="30B680A4914B3891" modified="2019-05-08" version="1">IHSLQAPSSLPSASFCILFIFLYLLTVLGITLFLQLQSQSPSQGQRISSPSDQQQLSPTDQGIWTVNSIGRLGNQMGEYATLYALAKMNGHQAYILPEMHQQLAPLFRITLPVLSSDVVQNIPWKDYELHDWMSEEYRHIEGKYVRLTGYPCSWTFYHHLRQEILQEFSFHDHVKEEANRYLAGLRGQRRNVTYVGVHIRRGDYVRVMPQVWKGVVADKAYLEKAMGYFRAKYQEPVFVVTSNGMEWCRENIDASRGDVYFSGDGEESSPGRDFALLAHCNHTIMTIGTFGIWAGYLAGGETVYLANYTLPDSPFLKIFKPEAAFLPEWIGINADLSPLQSETSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKV5</accession>
    <name>A0A452GKV5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dual specificity protein phosphatase 19</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Protein phosphatase SKRP1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Stress-activated protein kinase pathway-regulating phosphatase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Has a dual specificity toward Ser/Thr and Tyr-containing proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:20630"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:10685"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452GKV5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002410"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002765.1">
        <property type="protein sequence ID" value="ENSGAGP00000002410.1"/>
        <property type="gene ID" value="ENSGAGG00000001958.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000002769.1">
        <property type="protein sequence ID" value="ENSGAGP00000002414.1"/>
        <property type="gene ID" value="ENSGAGG00000001958.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008579">
        <property type="term" value="F:JUN kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14523">
        <property type="entry name" value="DSP_DUSP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000071">
        <property type="entry name" value="Dual specificity protein phosphatase 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46377">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46377:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="14">
        <location>
            <begin position="65"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="15">
        <location>
            <begin position="133"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="215" mass="24124" checksum="62663DF5A66731D8" modified="2019-05-08" version="1">MHSLTQEIRSFSRKNLKKQCTRVTTLTGKRIIETWKDARMQVVEEAEPSDGFGCGYVQDLSLDLQVGVIKPWLLLGSQDAAQDLETMKKYGITHVLNVAYGVENAFPNDFIYKNISILDLPETDITSYFPECFEFIEQAKMLDGVVLVHCNAGVSRAAAIIIGFLINSEGLNFARAFSWVKNARPAICPNPGFMEQLHKYQECSKKANGSIDDHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMP8</accession>
    <name>A0A452GMP8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Serine/threonine-protein kinase PLK</fullName>
            <ecNumber evidence="21">2.7.11.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Polo-like kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Microtubule organizing center</location>
            <location evidence="4">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.21" evidence="21"/>
    <dbReference type="AlphaFoldDB" id="A0A452GMP8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003103"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003571.1">
        <property type="protein sequence ID" value="ENSGAGP00000003103.1"/>
        <property type="gene ID" value="ENSGAGG00000002501.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13117">
        <property type="entry name" value="POLO_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14187">
        <property type="entry name" value="STKc_PLK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000727">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000003">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000284">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.30">
        <property type="entry name" value="POLO box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033702">
        <property type="entry name" value="PLK1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033695">
        <property type="entry name" value="POLO_box_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036947">
        <property type="entry name" value="POLO_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF93">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PLK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00659">
        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82615">
        <property type="entry name" value="Polo-box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 21" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 21" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="23">
        <location>
            <begin position="358"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="23">
        <location>
            <begin position="458"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU361162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="552" mass="62872" checksum="926DE3BD2A83377C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMQ9</accession>
    <name>A0A452GMQ9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMQ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002936"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003362.1">
        <property type="protein sequence ID" value="ENSGAGP00000002936.1"/>
        <property type="gene ID" value="ENSGAGG00000002343.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000759">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000206">
        <property type="entry name" value="zinc finger protein 202 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000352">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000320">
        <property type="entry name" value="Zinc finger protein 777"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381:SF393">
        <property type="entry name" value="CHROMATIN-LINKED ADAPTOR FOR MSL PROTEINS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="15">
        <location>
            <begin position="15"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="449"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="505"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="540" mass="62083" checksum="F2264B529A14AFC6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GND0</accession>
    <name>A0A452GND0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GND0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003359"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003870.1">
        <property type="protein sequence ID" value="ENSGAGP00000003359.1"/>
        <property type="gene ID" value="ENSGAGG00000002712.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016191">
        <property type="term" value="P:synaptic vesicle uncoating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11803">
        <property type="entry name" value="SH3_Endophilin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000053">
        <property type="entry name" value="endophilin-A1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035824">
        <property type="entry name" value="Endophilin_A_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF45">
        <property type="entry name" value="ENDOPHILIN-A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01887">
        <property type="entry name" value="SPECTRNALPHA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="93" mass="10707" checksum="3037FDC1946737D5" modified="2019-05-08" version="1">MDQPCCQALYDFEPENEGELGFKEGDIITLTNQIDENWYEGMLNGESGFFPINYVEVMVPLPQCLLVAHCCSTNVLLCSYCMWSLFKIILWLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPL5</accession>
    <name>A0A452GPL5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Max-binding protein MNT</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Myc antagonist MNT</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer with MAX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPL5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003847"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004460.1">
        <property type="protein sequence ID" value="ENSGAGP00000003847.1"/>
        <property type="gene ID" value="ENSGAGG00000003137.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11402">
        <property type="entry name" value="bHLHzip_Mnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000034">
        <property type="entry name" value="MAX network transcriptional repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11969">
        <property type="entry name" value="MAX DIMERIZATION, MAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11969:SF99">
        <property type="entry name" value="MAX-BINDING PROTEIN MNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="222"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="15"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="174"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="322"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="263"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="336"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="573" mass="62980" checksum="98112B1D2C731166" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPS3</accession>
    <name>A0A452GPS3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GPS3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003913"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004547.1">
        <property type="protein sequence ID" value="ENSGAGP00000003913.1"/>
        <property type="gene ID" value="ENSGAGG00000003203.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097110">
        <property type="term" value="F:scaffold protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017859">
        <property type="entry name" value="Treacle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20787">
        <property type="entry name" value="TREACLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20787:SF10">
        <property type="entry name" value="TREACLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="782"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1068"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="194"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="338"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="395"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="574"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="609"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="684"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="787"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="823"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="839"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="913"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="954"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1080"/>
            <end position="1090"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1090" mass="109864" checksum="674CD63B0B0CC75E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQA7</accession>
    <name>A0A452GQA7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein DEK</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in chromatin organization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Found in a mRNA splicing-dependent exon junction complex (EJC) with DEK, RBM8A, RNPS1, SRRM1 and ALYREF/THOC4. Interacts with histones H2A, H2B, H3, H4, acetylated histone H4, non-phosphorylated DAXX and HDAC2. Component of the B-WICH complex, at least composed of SMARCA5/SNF2H, BAZ1B/WSTF, SF3B1, DEK, MYO1C, ERCC6, MYBBP1A and DDX21. Binds DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQA7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004103"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004792.1">
        <property type="protein sequence ID" value="ENSGAGP00000004103.1"/>
        <property type="gene ID" value="ENSGAGG00000003375.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000779">
        <property type="term" value="P:regulation of double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000148">
        <property type="entry name" value="Dek, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044198">
        <property type="entry name" value="DEK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13468">
        <property type="entry name" value="DEK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13468:SF1">
        <property type="entry name" value="PROTEIN DEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEK-C" evidence="11">
        <location>
            <begin position="322"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="193"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="291"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="254"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="302"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="387" mass="44613" checksum="68516A2105BD849A" modified="2019-05-08" version="1">DAVIKMTVPLCSTEPPTMSSSKDDTMSAEQADEREPETENKVEESDEEEEEEEEEEKEKSLIVEGKREKKKVERLTMQVSSLQREPFTIAPGKGQKLCEIERIQFFLSKKKTDELRNLHKLLYNRPGTVASLKKNVGQFSGFPFEKGSDQYKKKEEMLKKFRNAMLKSICEVLDLERSGVNNELVTRILNFLMHPKPSGKPLPKSKKTPSKGGKKERSSSGTARKVKRTKCPEILSDESSSEEEDEKKEKAESSEEDKESEEEVNITKLPFPFQEKMNEFVLLSLKQQGDVKHSNKVNLPDLESESEDSSDDEPLIKKLKKPPTDEDLKETVKKLLANANLEEVTMKQICKEVYESYPSYDLSERKDFIKNTVKEVKTYLKKKWYFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQW1</accession>
    <name>A0A452GQW1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452GQW1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004315"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005071.1">
        <property type="protein sequence ID" value="ENSGAGP00000004315.1"/>
        <property type="gene ID" value="ENSGAGG00000003581.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010820">
        <property type="term" value="P:positive regulation of T cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06610">
        <property type="entry name" value="STKc_OSR1_SPAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000043">
        <property type="entry name" value="serine/threonine-protein kinase OSR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000114">
        <property type="entry name" value="serine/threonine-protein kinase OSR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000068">
        <property type="entry name" value="STE20/SPS1-related proline-alanine-rich protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024678">
        <property type="entry name" value="Kinase_OSR1/WNK_CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF14">
        <property type="entry name" value="STE20_SPS1-RELATED PROLINE-ALANINE-RICH PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12202">
        <property type="entry name" value="OSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="27"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="328"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="367"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="529" mass="59519" checksum="1B4611EE72DB167F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSV4</accession>
    <name>A0A452GSV4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GSV4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004980"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005818.1">
        <property type="protein sequence ID" value="ENSGAGP00000004980.1"/>
        <property type="gene ID" value="ENSGAGG00000003996.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000218">
        <property type="entry name" value="Zinc finger protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000011">
        <property type="entry name" value="zinc finger protein 615 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF218">
        <property type="entry name" value="ZINC FINGER PROTEIN 502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="230"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="512" mass="58605" checksum="BD0DC3335FB8AD89" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTV3</accession>
    <name>A0A452GTV3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Calcium/calmodulin-dependent protein kinase type 1G</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">CaM kinase I gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">CaMK-like CREB kinase III</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTV3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005461"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006357.1">
        <property type="protein sequence ID" value="ENSGAGP00000005461.1"/>
        <property type="gene ID" value="ENSGAGG00000004430.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14166">
        <property type="entry name" value="STKc_CaMKI_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000026">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000331">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11 21 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 21 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0636">Prenylation</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 22" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="23"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00071781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00083144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="467" mass="52600" checksum="74BCB2C0B752AE4F" modified="2019-05-08" version="1">MGRKEVDDTRSWKKPTNNIRKTFIFMEALGSGAFSEVFLVKQRITGKLFALKCIKKSPVIQDSSLENEIAVLKKIKHENIVTLEDIYESGTHFYLVMQLVSGGELFDRILERGVYTEKDASVVIQQVLAAVKYLHENGIVHRDLKPENLLYLTPEENSKIMITDFGLSKMEQNGIMSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVITYILLCGYPPFYEETESKLFEKIKEGYYEFESPFWDDISESAKDFISHLLEKDPNERFTCERALRHPWIDGNTALHRDIYPSVSAQIQKNFAKSKWKQAFNAAAVVRHMKKLHMTCHAQADMSLPIIQVSEVSRLSSPMGTSQEIPIQDKKMLIPMLACQIPPSQLDQNTQMTQSKSLDHLVNESLILPDNQSPPTLTSCGCSSASEGHEKVKTVFCSESALLKKDAKNHHFKSEVIVPMKTSNNSYRGTGQTGVCLVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUH0</accession>
    <name>A0A452GUH0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GUH0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005412"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006303.1">
        <property type="protein sequence ID" value="ENSGAGP00000005412.1"/>
        <property type="gene ID" value="ENSGAGG00000004394.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12888">
        <property type="entry name" value="SPRY_PRY_TRIM7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="1">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="177" mass="20211" checksum="0E279CBDD3E96122" modified="2019-05-08" version="1">MQRSRSPGGRVIAILDPDTAHPSLLISENRRSVKWRGLQQDVPDNPERFDSETCVLGLEGFTSGQHYWEVEVRSGRTWAVGVARESVRRKGWINFSPEEMIWAVDQCGSHYRARISPETLLPLTGSPGRIGVYLDYEWGLVSFYHPGMEARIYIFTTSFTGQLQPFFWVYSPITLCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVS2</accession>
    <name>A0A452GVS2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 17">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="11 17">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 17">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11 17"/>
    <dbReference type="AlphaFoldDB" id="A0A452GVS2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006087"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007093.1">
        <property type="protein sequence ID" value="ENSGAGP00000006087.1"/>
        <property type="gene ID" value="ENSGAGG00000004945.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000007100.1">
        <property type="protein sequence ID" value="ENSGAGP00000006094.1"/>
        <property type="gene ID" value="ENSGAGG00000004945.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000007106.1">
        <property type="protein sequence ID" value="ENSGAGP00000006100.1"/>
        <property type="gene ID" value="ENSGAGG00000004945.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02658">
        <property type="entry name" value="Peptidase_C19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14383">
        <property type="entry name" value="UBA1_UBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14386">
        <property type="entry name" value="UBA2_UBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000016">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000070">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000026">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000256">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000033">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000042">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016652">
        <property type="entry name" value="Ubiquitinyl_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041812">
        <property type="entry name" value="UBP5_UBA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF103">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016308">
        <property type="entry name" value="UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 11 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11 17" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11 17" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 11 17" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10 11 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="21">
        <location>
            <begin position="174"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="20">
        <location>
            <begin position="325"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="19">
        <location>
            <begin position="653"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="19">
        <location>
            <begin position="721"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="817"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="220"/>
            <end position="223"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="194"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="857" mass="95871" checksum="482D5C297186A22F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWD3</accession>
    <name>A0A452GWD3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel HCN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GWD3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006313"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007338.1">
        <property type="protein sequence ID" value="ENSGAGP00000006313.1"/>
        <property type="gene ID" value="ENSGAGG00000005085.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000031">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000002">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000007">
        <property type="entry name" value="Putative potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013621">
        <property type="entry name" value="Ion_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF4">
        <property type="entry name" value="POTASSIUM_SODIUM HYPERPOLARIZATION-ACTIVATED CYCLIC NUCLEOTIDE-GATED CHANNEL 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08412">
        <property type="entry name" value="Ion_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0114">cAMP</keyword>
    <keyword evidence="7" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="19" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="16 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="4" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="8 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="440"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="25">
        <location>
            <begin position="543"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="88"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="788"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="835"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="984"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="23">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="788"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="840"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="850"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="891"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="953"/>
            <end position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1182" mass="128782" checksum="39B76894026FFF6F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWI0</accession>
    <name>A0A452GWI0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Little elongation complex subunit 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GWI0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006455"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007512.1">
        <property type="protein sequence ID" value="ENSGAGP00000006455.1"/>
        <property type="gene ID" value="ENSGAGG00000005223.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045945">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019535">
        <property type="entry name" value="ICE2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14633">
        <property type="entry name" value="LITTLE ELONGATION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14633:SF3">
        <property type="entry name" value="LITTLE ELONGATION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10505">
        <property type="entry name" value="NARG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Little elongation complex subunit 2 C-terminal" evidence="2">
        <location>
            <begin position="458"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="665"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="682"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="713"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="732" mass="82051" checksum="ADBCE32A1ED7BBF9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWZ3</accession>
    <name>A0A452GWZ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hydroxymethylglutaryl-CoA lyase, mitochondrial</fullName>
            <ecNumber evidence="6">4.1.3.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">3-hydroxy-3-methylglutarate-CoA lyase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Mitochondrial 3-hydroxy-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(3S)-3-hydroxy-3-methylglutaryl-CoA = acetoacetate + acetyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:24404"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="4.1.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate metabolism; (S)-3-hydroxy-3-methylglutaryl-CoA degradation; acetoacetate from (S)-3-hydroxy-3-methylglutaryl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer; disulfide-linked. Can also form homotetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HMG-CoA lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.4" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452GWZ3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006616"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007691.1">
        <property type="protein sequence ID" value="ENSGAGP00000006616.1"/>
        <property type="gene ID" value="ENSGAGG00000005346.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00896">
        <property type="reaction ID" value="UER00863"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004419">
        <property type="term" value="F:hydroxymethylglutaryl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046951">
        <property type="term" value="P:ketone body biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07938">
        <property type="entry name" value="DRE_TIM_HMGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000038">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA lyase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000138">
        <property type="entry name" value="HMG_CoA_lyase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043594">
        <property type="entry name" value="HMGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004283">
        <property type="entry name" value="PRK05692.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42738">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42738:SF1">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA LYASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01062">
        <property type="entry name" value="HMG_COA_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="18">
        <location>
            <begin position="30"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="322" mass="33919" checksum="82E1BC24E0D3E042" modified="2019-05-08" version="1">MVAAKRALPWLAASLRPISSTAAGSLPKQVKIVEVGPRDGLQNEKNIVPTQVKICLINMLSETGLPVIEATSFVSPKWVPQMADHTEVLQGIQKFPGVSYPVLTPNLKGFQAAVAAGAKEVSIFGAASELFTKKNINCSIEESLQRFSEVMHAAGEASIPVRGYVSCVLGCPYEGKIAPAKVAEVSKKMYSMGCYEISLGDTIGVGTPGSMKEMLSAVMKEVPVRALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPYAQGASGNVATEDLVYMLHGLGIHTGVDPQKLMEAGVFICKALNRRTSSKVVQASSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZP9</accession>
    <name>A0A452GZP9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GZP9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007558"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008726.1">
        <property type="protein sequence ID" value="ENSGAGP00000007558.1"/>
        <property type="gene ID" value="ENSGAGG00000006045.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019087582" description="Secreted protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="61" mass="6784" checksum="04706CB6E0ABAFAD" modified="2019-05-08" version="1">MCCLEKTGRKAKVPVLFFALAPGASAARNLVSVGTDQTWMRREQLSSKRQFVFKEPAEHGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H093</accession>
    <name>A0A452H093_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">rhomboid protease</fullName>
            <ecNumber evidence="4">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452H093"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007969"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009184.1">
        <property type="protein sequence ID" value="ENSGAGP00000007969.1"/>
        <property type="gene ID" value="ENSGAGG00000006343.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000002">
        <property type="entry name" value="Rhomboid, veinlet-like 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000194">
        <property type="entry name" value="rhomboid-related protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017213">
        <property type="entry name" value="Peptidase_S54_rhomboid_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051739">
        <property type="entry name" value="Rhomboid_IM_Serine_Proteases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840:SF5">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037470">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="355"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="65"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037470-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="389" mass="43749" checksum="6909D7512FE6BA76" modified="2019-05-08" version="1">MGDRRPETRASSSPAVAASSEAEEIEVLESEDVLPMATEDFDPENTGYISTEKFRNLLQRHGSELDPHKLEVLLALADNNSDGKICYQDFVNLMSNKRSNSFRQAILQGNRRLCSKALLEESGLSLSQRLIRHVAYETLPREIDRKWYYDSYTCCPPPWFMIAITIVEVAFFLYNGVALDRFVLQVTHPLYLKNSLLYHPQLRAQAWRIEHLGLNVALQLLVGVPLEMVHGAARIGFVYIAGVVAGSLAVSVADMTAPVVGSSGGVYALISAHLANIVMNWSGMKCQFKLLRMALALICMSFEFGRAVWLRFHPSAYPPCPHPSFVAHLGGVLVGITLGVVILRNYEQRLQDQSLWWIFIFIYVVFVLFAIFWNVFAYNLLDLKLPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0G1</accession>
    <name>A0A452H0G1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H0G1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007918"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009126.1">
        <property type="protein sequence ID" value="ENSGAGP00000007918.1"/>
        <property type="gene ID" value="ENSGAGG00000006310.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15129">
        <property type="entry name" value="7tmA_GPR142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052477">
        <property type="entry name" value="Orphan_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46272:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 142-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46272">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="232"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="48"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="335" mass="38094" checksum="29E742A4F547199C" modified="2019-05-08" version="1">MMFLLPNSTDGAWVSDAEPLEWEEWSPCMEGIIPVIYYSVLLGLGLPINILTAIALSRLATRTKKSSYWYLLALTASDILTQVIIVFMGFILQMAILAHKVPHAFLHTVNVLQFAANHASIWVTVLLTADRYVALCHPLRYRMLSYPERTRKIIAAVFGTALATGIPFYWWLDIWHDSQTPTTVDKVLKWVHCFTMYFIPCSIFLATNSVIICKLKWGNRVDGHHRRVGKTIAMLMAVTAIFTVLWAPRTFVILFHLYMGSTNKNRRLHVALNLANMIAMLNTTVNFFFYCFVSKTFRRTAGQVIRSCSPFCKPTNRGFLHSALKPLGLLENVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2I7</accession>
    <name>A0A452H2I7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Receptor-interacting serine/threonine-protein kinase 1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Cell death protein RIP</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Receptor-interacting protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">(Microbial infection) Interacts with Murid herpesvirus 1 protein RIR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452H2I7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008621"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009913.1">
        <property type="protein sequence ID" value="ENSGAGP00000008621.1"/>
        <property type="gene ID" value="ENSGAGG00000006833.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004706">
        <property type="term" value="F:JUN kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097191">
        <property type="term" value="P:extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097527">
        <property type="term" value="P:necroptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001237">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001238">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050729">
        <property type="term" value="P:positive regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070105">
        <property type="term" value="P:positive regulation of interleukin-6-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042327">
        <property type="term" value="P:positive regulation of phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060544">
        <property type="term" value="P:regulation of necroptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000377">
        <property type="term" value="P:regulation of reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097343">
        <property type="term" value="P:ripoptosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033209">
        <property type="term" value="P:tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000052">
        <property type="entry name" value="Putative receptor-interacting serine/threonine-protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000472">
        <property type="entry name" value="Receptor interacting serine/threonine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF6">
        <property type="entry name" value="RECEPTOR-INTERACTING SERINE_THREONINE-PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="18" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1210">Necrosis</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="43"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="27">
        <location>
            <begin position="655"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="525"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="551"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00062158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00071068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00081899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00083512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="743" mass="84689" checksum="191553D79FB5DFC8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2Q0</accession>
    <name>A0A452H2Q0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H2Q0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008796"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010112.1">
        <property type="protein sequence ID" value="ENSGAGP00000008796.1"/>
        <property type="gene ID" value="ENSGAGG00000006956.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22519">
        <property type="entry name" value="KH-I_PCBP3_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000002">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="92"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="241" mass="25509" checksum="6B86DF0788762940" modified="2019-05-08" version="1">MESKVSEGGLNVTLTIRLLMHGKVRRGCTFCCLHFSSISGARINISEGNCPERIVTITGPTDAIFKAFAMIAYKFEEDIINSMSNSTATSKPPVTLRLVVPASQCGSLIGKGGSKIKEIRESTGAQVQVAGDMLPNSTERAVTISGTPDAIIQCVKQICVVMLESPPKGATIPYRPKPASTPVIFAGGQVRADPLAASTANLSLLLQHQPLPVSSPAQHPQLQFLAQVLPPISSPTIPPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H379</accession>
    <name>A0A452H379_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kelch repeat and BTB domain-containing protein 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the KBTBD8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H379"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008867"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010193.1">
        <property type="protein sequence ID" value="ENSGAGP00000008867.1"/>
        <property type="gene ID" value="ENSGAGG00000007016.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014032">
        <property type="term" value="P:neural crest cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014029">
        <property type="term" value="P:neural crest formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18483">
        <property type="entry name" value="BACK_KBTBD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18274">
        <property type="entry name" value="BTB_POZ_KBTBD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000006">
        <property type="entry name" value="Kelch repeat and BTB domain-containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000014">
        <property type="entry name" value="kelch repeat and BTB domain-containing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000020">
        <property type="entry name" value="Kelch repeat and BTB domain-containing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028764">
        <property type="entry name" value="BTB/POZ_KBTBD8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF433">
        <property type="entry name" value="KELCH REPEAT AND BTB DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="12">
        <location>
            <begin position="49"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="601" mass="68900" checksum="8E7F8B47834DA71C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3A2</accession>
    <name>A0A452H3A2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aquaporin 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3A2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008889"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010215.1">
        <property type="protein sequence ID" value="ENSGAGP00000008889.1"/>
        <property type="gene ID" value="ENSGAGG00000007024.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015670">
        <property type="term" value="P:carbon dioxide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF113">
        <property type="entry name" value="AQUAPORIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="195"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="34551" checksum="7A98071A479DCE19" modified="2019-05-08" version="1">MWKEVLSVTFYRAVFIEFLATSIYVFFGLGSVLQWPSALPTILQISITFNLAAATVVQIAWHVSGAHVNPAVTMAFLLGSRISLARAACYMVAQLAGGIAGAATLYGVTPAEVQGSLGINTSLCHPFPQVRSNITSGQAVAVELILTFQLVLCYFASTDRHRNANSPATFIGISVALGHLIGGFVPNGLLAVGQEQGRSTGPRLPRGPHTIHTAGPAAPQAPEAPPPISLELLTVSPLPRVSAPTSRPGPSPLCCGASPCMVSLISSWASLPRGSGPGSFLGFPAIPSGLLSWLCRLGSFNCSPGGCRSAPCSGSTSPQDLAPPFQGSLSLLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3E2</accession>
    <name>A0A452H3E2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial import receptor subunit TOM70</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Mitochondrial precursor proteins import receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Translocase of outer membrane 70 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Translocase of outer mitochondrial membrane protein 70</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the Tom70 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3E2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009065"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010403.1">
        <property type="protein sequence ID" value="ENSGAGP00000009065.1"/>
        <property type="gene ID" value="ENSGAGG00000007156.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000141">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000071">
        <property type="entry name" value="Putative mitochondrial import receptor subunit TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 19" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="121"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="374"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="408"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="483"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="552"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="79"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="615" mass="67977" checksum="928E2DCEFDE19C02" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3K5</accession>
    <name>A0A452H3K5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>H(+)(in) = H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34979"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 17">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3K5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009146"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010505.1">
        <property type="protein sequence ID" value="ENSGAGP00000009146.1"/>
        <property type="gene ID" value="ENSGAGG00000007228.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901029">
        <property type="term" value="P:negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000002">
        <property type="entry name" value="Putative ADP/ATP translocase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11 15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="35"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="140"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="241"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="327" mass="35812" checksum="EC031A588F4A264C" modified="2019-05-08" version="1">MRGDDPALFCLCVAVAAFFPLNGSRLSTNMADQALSFAKDFLAGGVAAAISKTAVAPIERVKLLLQVQHASKQIAADKQYKGIIDCVVRIPKEQGILSFWRGNLANVIRYFPTQALNFAFKDKYKQVFLGGVDKHTQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKAGADREFRGLGDCLVKITKSDGLRGLYQGFNVSVQGIIIYRAAYFGIYDTAKGMLPDPRNTHIVISWMIAQTVTSVAGVVSYPFDTVRRRMMMQSGRKGADIMYSGTIDCWKKIARDEGGKAFFKGAWSNVLRGMGGAFVLVLYDELKKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3T9</accession>
    <name>A0A452H3T9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H3T9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009248"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010616.1">
        <property type="protein sequence ID" value="ENSGAGP00000009248.1"/>
        <property type="gene ID" value="ENSGAGG00000007306.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044306">
        <property type="term" value="C:neuron projection terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028063">
        <property type="entry name" value="SCRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17463:SF0">
        <property type="entry name" value="SCRAPIE-RESPONSIVE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17463">
        <property type="entry name" value="SCRAPIE-RESPONSIVE PROTEIN 1 SCRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15224">
        <property type="entry name" value="SCRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019517591" evidence="1">
        <location>
            <begin position="22"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="100" mass="11344" checksum="5F46D3DBDFC504AA" modified="2019-05-08" version="1">MMKMKILSALVLLSMLLGTNMMPFSRLSCYKKVLKDRNCHSIPEGVASLQGIDGNLQDHFWEGKSCEMICYCNFSELLCCPKDIFFGPKISFLIPCNNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3Z2</accession>
    <name>A0A452H3Z2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H3Z2"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010694.1">
        <property type="protein sequence ID" value="ENSGAGP00000009308.1"/>
        <property type="gene ID" value="ENSGAGG00000007364.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019815">
        <property type="term" value="C:B cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030183">
        <property type="term" value="P:B cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050853">
        <property type="term" value="P:B cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14334">
        <property type="entry name" value="B-CELL ANTIGEN RECEPTOR COMPLEX-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="194" mass="21262" checksum="B828A90375349052" modified="2025-10-08" version="2">MIKTSVVLVVLTPPSIRAMEGSEFTIKCTFNTSNNSTRWFVEWNKIRNNVTEKVSNGTDQIISADKEKRSLSLTVKKASVTDCGTYVCRVGSKDVIYPGTGTQVTIEGKYQHFLLCSTNPSGSSGHWAGSRELGQVVLGELEQHKVRMSLFGEGGFECIEVCIPVCLLGAYSTVSECLAVDHRFLGYLGWLLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4C3</accession>
    <name>A0A452H4C3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H4C3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009509"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010925.1">
        <property type="protein sequence ID" value="ENSGAGP00000009509.1"/>
        <property type="gene ID" value="ENSGAGG00000007523.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015252">
        <property type="term" value="F:proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042472">
        <property type="term" value="P:inner ear morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004878">
        <property type="entry name" value="Otopetrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522">
        <property type="entry name" value="PROTON CHANNEL OTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522:SF19">
        <property type="entry name" value="PROTON CHANNEL OTOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03189">
        <property type="entry name" value="Otopetrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="356" mass="40824" checksum="36B8BB25AA29F735" modified="2019-05-08" version="1">DKIEPVFSLKQLVEAIIHSLVEKWKGNCFLNILFSIKILVIFKCKYIKINYPITYTFLDDHAPQCNCTTTLCSIFSQGMYYLYPFNIEYHILASTMLYVLWKNIGRKVEHHHQHKILFKFHGITVGTIFGLIVLISTIAVVVVYLIQIGRSKIKSELALTMFYLYAISVLALMCAAGIVALLIYRLEERSMDNSKNPARKLDADLLVGTASGSWLLSWGSILAIICAQAHPKYTWYNLPYSILVIIEKYIQNLFIIESIHREQEKVNDDIKTLRIVTVSTSSPRRILKNIAAFLFLCNLSLWIPPAFGCRPEYDNGLEEIVFGFEPWIIVVNLAMPFSIFYRMHSAASLFEVNCKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4W1</accession>
    <name>A0A452H4W1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">choline-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="11">2.7.7.15</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>phosphocholine + CTP + H(+) = CDP-choline + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18997"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="2.7.7.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:18998"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.15" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A452H4W1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009542"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010968.1">
        <property type="protein sequence ID" value="ENSGAGP00000009542.1"/>
        <property type="gene ID" value="ENSGAGG00000007544.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00753">
        <property type="reaction ID" value="UER00739"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000016">
        <property type="entry name" value="Putative choline-phosphate cytidylyltransferase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF20">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="15">
        <location>
            <begin position="80"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="312"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="369" mass="41964" checksum="5C5B87545610EF1E" modified="2019-05-08" version="1">MPVLTTDAESETGIPKSLSNELLSEIMEEIEHTCPQPRLTLIAPAPFADEISCQCQAPHEKLTITQARLGTPVDRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNETERYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPYSSAGSDDVYKHIKEAGMFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKKYRFQNQVDKMKEKVKNVEEKSKEFVNKVEEKSHDLIQKWEEKSREFIGNFLELFGPDGAWKQMFQERSGRMLQALSPRQSPTSSPTRGRSPSRSPSPPSPWLFNKASPPSSPKVASASISSMSEGDEDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6L1</accession>
    <name>A0A452H6L1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Interacts with beta-1 and beta-2, but not with beta-3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">G proteins are composed of 3 units, alpha, beta and gamma.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Lipid-anchor</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H6L1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010344"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011876.1">
        <property type="protein sequence ID" value="ENSGAGP00000010344.1"/>
        <property type="gene ID" value="ENSGAGG00000008111.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000007">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="13">
        <location>
            <begin position="28"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="68" mass="7169" checksum="D7980E31C7D0FDC0" modified="2019-05-08" version="1">MVGSTAYVAMCRADEVQGEAGTNYQGLVSQAAAELQQYCMQNACKDALLIGVPAGSNPFREPRSCALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6Q6</accession>
    <name>A0A452H6Q6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSGAGT00000011898.1">
        <property type="protein sequence ID" value="ENSGAGP00000010363.1"/>
        <property type="gene ID" value="ENSGAGG00000008126.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF164">
        <property type="entry name" value="MAM DOMAIN-CONTAINING GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="137"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="247"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="353" mass="38271" checksum="4AE06AEA468C9C2D" modified="2019-05-08" version="1">MPQMHGSTGAPHSRPAAPASPALPPAHTLCVVCWNCTITCSCWESVTGGLVLTFRPREGPGPWRLCSVPPAGALRGRVRGRRNPDXPPPPRRLPASAATRKPDARVCLEPSPAAMARPGPLLLLAALALRWAHGEIPTVRLIDQEGPILEGSYVTLECLTDEAGANMSQFTFQKYSRWLHSWVSLDNPDRLRCWFFDVNVTRADGRLLLAIGALQSWHAGAYRCAAANSTGNAAASAELSLRVEYLRSVFVTRAHTWCGTVGDSVAVMEGEDVELQCSADASQPPQYEWSRQGADWVVASSVLTLPQVSREQAGTYECRAQHPSLPHLARSRAVRLDVQGPQSSYQLGESMAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H744</accession>
    <name>A0A452H744_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Seizure 6-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the SEZ6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H744"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010382"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011918.1">
        <property type="protein sequence ID" value="ENSGAGP00000010382.1"/>
        <property type="gene ID" value="ENSGAGG00000008035.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021680">
        <property type="term" value="P:cerebellar Purkinje cell layer development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090036">
        <property type="term" value="P:regulation of protein kinase C signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060074">
        <property type="term" value="P:synapse maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000025">
        <property type="entry name" value="seizure 6-like protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000009">
        <property type="entry name" value="Seizure related 6 homolog like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000010">
        <property type="entry name" value="Seizure related 6 homolog like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000012">
        <property type="entry name" value="Seizure related 6 homolog like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000030">
        <property type="entry name" value="Seizure related 6 homolog like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051277">
        <property type="entry name" value="SEZ6_CSMD_C4BPB_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656:SF8">
        <property type="entry name" value="SEIZURE 6-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2 14" id="KW-0768">Sushi</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="968"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="17">
        <location>
            <begin position="293"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="18">
        <location>
            <begin position="403"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="17">
        <location>
            <begin position="464"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="18">
        <location>
            <begin position="576"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="17">
        <location>
            <begin position="639"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="18">
        <location>
            <begin position="754"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="18">
        <location>
            <begin position="815"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="18">
        <location>
            <begin position="882"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="53"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="784"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1035" mass="113603" checksum="532B9F6BB7CD8D48" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H773</accession>
    <name>A0A452H773_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H773"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010542"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012103.1">
        <property type="protein sequence ID" value="ENSGAGP00000010542.1"/>
        <property type="gene ID" value="ENSGAGG00000008242.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000055">
        <property type="entry name" value="RNA exonuclease 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF82">
        <property type="entry name" value="RNA EXONUCLEASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="521"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="616"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="789" mass="89640" checksum="6001395CCAEE99D9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H783</accession>
    <name>A0A452H783_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IRG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H783"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010406"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011945.1">
        <property type="protein sequence ID" value="ENSGAGP00000010406.1"/>
        <property type="gene ID" value="ENSGAGG00000008149.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000541">
        <property type="entry name" value="Immunity related GTPase M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051515">
        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341:SF17">
        <property type="entry name" value="IRG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRG-type G" evidence="5">
        <location>
            <begin position="46"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="413" mass="45921" checksum="CD64498BD398B028" modified="2019-05-08" version="1">MAGLDAKELPKIPEEDMEALKAAVGEGNLTKAAAKAKEALELNDKIPLNIAVTGESGSGKSSFVNAIRGLEDTDKDASETGVNEMTMKPNAYPHPTYPNVIVWDLPGIGTPEFKSDTYLKDMKFNHYDFFIIISCTRFKSHDIQLAQEIQRQGKKFYFVRSKVDADLTNEKRHYDEEGILKQRDAPTKRPYHYSESVILDNIREKCIESLKRGGVASPQVFLLSRWDLGKYDFPKLQETLVDELPSHKRLALLRSLSNVSKETLQKKKEQLQTQIYLKSLVSCGVAALPIPGLSTVCDIAMLVTSLKEYCRDFGLSENALATLARQANKPIEELKAVIKSPLSEEITKDLAFKLLTKYATGAVKYSTYLFGFVPVVGSVVSAAGSLSTTYFMLKNFLDDVAADAVRVLEVTMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8T2</accession>
    <name>A0A452H8T2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CEP152 CEP63 binding coiled coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000011149"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012770.1">
        <property type="protein sequence ID" value="ENSGAGP00000011149.1"/>
        <property type="gene ID" value="ENSGAGG00000008628.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007099">
        <property type="term" value="P:centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051235">
        <property type="entry name" value="CEP152/SHC-Transforming"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057659">
        <property type="entry name" value="CEP152_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057664">
        <property type="entry name" value="CEP152_PLK4_bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337:SF6">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 152 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337">
        <property type="entry name" value="SHC TRANSFORMING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25770">
        <property type="entry name" value="CC_CEP63-bind_CEP152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25769">
        <property type="entry name" value="PLK4_bind_CEP152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CEP152 CEP63 binding coiled coil" evidence="3">
        <location>
            <begin position="1387"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1556"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1665"/>
            <end position="1697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1775"/>
            <end position="1809"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="268"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="356"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="577"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="646"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="718"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="910"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1027"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1293"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1561"/>
            <end position="1573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1600"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1775"/>
            <end position="1786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1794"/>
            <end position="1805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1839" mass="212703" checksum="34A90854B4D87F98" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8Z6</accession>
    <name>A0A452H8Z6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">RNA binding motif protein 4B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H8Z6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011046"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012648.1">
        <property type="protein sequence ID" value="ENSGAGP00000011046.1"/>
        <property type="gene ID" value="ENSGAGG00000008553.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12606">
        <property type="entry name" value="RRM1_RBM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12607">
        <property type="entry name" value="RRM2_RBM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000058">
        <property type="entry name" value="RNA-binding motif protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000085">
        <property type="entry name" value="RNA-binding protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034897">
        <property type="entry name" value="RBM4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034898">
        <property type="entry name" value="RBM4_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF26">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN M-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="2"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="78"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="14">
        <location>
            <begin position="162"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="41444" checksum="307B25BE1A0F90EB" modified="2019-05-08" version="1">MVKLFIGNLPREATEQEIRSLFEQYGKVLECDIIKNYGFVHIEDKTAAEDAIRNLHHHKLHGVCINVEASKNKSKASTKLHVGNISTTCTNLELRAKFEEYGPVIECDIVKDYAFVHMERAEDAVEAIRGLDNTEFQGKRMHVQLSTSRLRTAPGMGDKSGCYRCGKEGHWSKECPVDRTGQVPDFTETYNEQYGAVRTPYPAGYGETMYYDDGYGAMVDYYKRYRVRPYATASAYDAYAEQTMAQYSQYAQYSQVQTTAMAATTAMAATTAMANRLTATLDPYDRALLPTPGAAAAAVAAAATAAAAAASSTYYTRDRSPLRRTATAATTVGEAYGYERCQLSPVSSVARASLYDVQRFGRESYADRARYSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAX1</accession>
    <name>A0A452HAX1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Allantoate amidinohydrolase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the allantoicase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HAX1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011893"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013623.1">
        <property type="protein sequence ID" value="ENSGAGP00000011893.1"/>
        <property type="gene ID" value="ENSGAGG00000009170.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004037">
        <property type="term" value="F:allantoicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000256">
        <property type="term" value="P:allantoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000077">
        <property type="entry name" value="Probable allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00813">
        <property type="entry name" value="Allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005164">
        <property type="entry name" value="Allantoicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015908">
        <property type="entry name" value="Allantoicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02961">
        <property type="entry name" value="allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12045">
        <property type="entry name" value="ALLANTOICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12045:SF3">
        <property type="entry name" value="INACTIVE ALLANTOICASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03561">
        <property type="entry name" value="Allantoicase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016516">
        <property type="entry name" value="Allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Allantoicase" evidence="3">
        <location>
            <begin position="7"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Allantoicase" evidence="3">
        <location>
            <begin position="173"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00031078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="345" mass="39272" checksum="6837DEE056BCE6C1" modified="2019-05-08" version="1">VWLSPVLKPDEFTDYGKWMDGWETRRKRIPGHDWCIIQLGVPGIVHGFEVNTSFFAGNSAPRISIQAALDLPEFWPRGDRIGTAASDEELKAVKKMKSDEWTLLVPMKELKPRYLDTSHNYFSASCKRKWTHIRLNIYPDGGIARFKVYGAVQKDWSASGPNDLDDLVAMENGGICVGFSSAQLGHPRNILGLGRATSMEDGWETARRPDRPPVLKADEKGILLMPGNEWAVFRLAHPCVITHIEIDTNHFKGNFPDTCKLEACVLNTEEEKEYITQKWNLKQNPKWNPLLSFTKLKPNKRHFFDGTALQMQDVITHVRLVIVPDGGVSRMRLWGFPRTLLYTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBB9</accession>
    <name>A0A452HBB9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Pappalysin-1</fullName>
            <ecNumber evidence="18">3.4.24.79</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Insulin-like growth factor-dependent IGF-binding protein 4 protease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Pregnancy-associated plasma protein A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Cleavage of the 135-Met-|-Lys-136 bond in insulin-like growth factor binding protein (IGFBP)-4, and the 143-Ser-|-Lys-144 bond in IGFBP-5.</text>
            <dbReference type="EC" id="3.4.24.79"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homodimer; disulfide-linked. In pregnancy serum, predominantly found as a disulfide-linked 2:2 heterotetramer with the proform of PRG2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M43B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.79" evidence="18"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012054"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013804.1">
        <property type="protein sequence ID" value="ENSGAGP00000012054.1"/>
        <property type="gene ID" value="ENSGAGG00000009279.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd04275">
        <property type="entry name" value="ZnMc_pappalysin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000045">
        <property type="entry name" value="Pappalysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000057">
        <property type="entry name" value="Pappalysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000061">
        <property type="entry name" value="Pappalysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000068">
        <property type="entry name" value="Pappalysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000097">
        <property type="entry name" value="Pappalysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000026">
        <property type="entry name" value="Pappalysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006558">
        <property type="entry name" value="LamG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000800">
        <property type="entry name" value="Notch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043543">
        <property type="entry name" value="PAPPA/PAPPA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058897">
        <property type="entry name" value="PAPPA_SD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008754">
        <property type="entry name" value="Peptidase_M43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46130">
        <property type="entry name" value="LAMGL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46130:SF2">
        <property type="entry name" value="PAPPALYSIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25900">
        <property type="entry name" value="PAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05572">
        <property type="entry name" value="Peptidase_M43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00560">
        <property type="entry name" value="LamGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00004">
        <property type="entry name" value="NL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 24" id="KW-0732">Signal</keyword>
    <keyword evidence="5 22" id="KW-0768">Sushi</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019422114" description="Pappalysin-1" evidence="24">
        <location>
            <begin position="19"/>
            <end position="1611"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="25">
        <location>
            <begin position="1283"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="25">
        <location>
            <begin position="1413"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="1415"/>
            <end position="1458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00067550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00075042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00077848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1611" mass="180238" checksum="F8A563888FDAB18C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCK1</accession>
    <name>A0A452HCK1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000012506"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014332.1">
        <property type="protein sequence ID" value="ENSGAGP00000012506.1"/>
        <property type="gene ID" value="ENSGAGG00000009595.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000014336.1">
        <property type="protein sequence ID" value="ENSGAGP00000012510.1"/>
        <property type="gene ID" value="ENSGAGG00000009595.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046974">
        <property type="term" value="F:histone H3K9 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20905">
        <property type="entry name" value="EHMT_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10535">
        <property type="entry name" value="SET_EHMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000005">
        <property type="entry name" value="Euchromatic histone-lysine N-methyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043550">
        <property type="entry name" value="EHMT1/EHMT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047762">
        <property type="entry name" value="EHMT_CRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038035">
        <property type="entry name" value="SET_EHMT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46307">
        <property type="entry name" value="G9A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46307:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE EHMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21533">
        <property type="entry name" value="EHMT1-2_CRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="784"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="817"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="902"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="936"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="1002"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-SET" evidence="9">
        <location>
            <begin position="1124"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="8">
        <location>
            <begin position="1190"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="47"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="202"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="337"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="679"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1333"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="408"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="455"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="683"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1333"/>
            <end position="1357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1363" mass="151006" checksum="8E08B82AA8E98D68" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HD61</accession>
    <name>A0A452HD61_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Pleckstrin homology domain-containing family E member 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Suprachiasmatic nucleus circadian oscillatory protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 25">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012762"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014628.1">
        <property type="protein sequence ID" value="ENSGAGP00000012762.1"/>
        <property type="gene ID" value="ENSGAGG00000009793.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000014634.1">
        <property type="protein sequence ID" value="ENSGAGP00000012767.1"/>
        <property type="gene ID" value="ENSGAGG00000009793.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13322">
        <property type="entry name" value="PH_PHLPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000278">
        <property type="entry name" value="PH domain and leucine rich repeat protein phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000345">
        <property type="entry name" value="PH domain and leucine rich repeat protein phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000027">
        <property type="entry name" value="PH domain and leucine rich repeat protein phosphatase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000003">
        <property type="entry name" value="PH domain and leucine-rich repeat protein phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055071">
        <property type="entry name" value="RA_PHLPP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF43">
        <property type="entry name" value="PH DOMAIN AND LEUCINE RICH REPEAT PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23010">
        <property type="entry name" value="RA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="22">
        <location>
            <begin position="1142"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="234"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="333"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1445"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1567"/>
            <end position="1594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1612"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1643"/>
            <end position="1656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00080105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1665" mass="182410" checksum="5D907169FEBCA370" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFD2</accession>
    <name>A0A452HFD2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFD2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013544"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015505.1">
        <property type="protein sequence ID" value="ENSGAGP00000013544.1"/>
        <property type="gene ID" value="ENSGAGG00000010295.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00108">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000069">
        <property type="entry name" value="Hyaluronan-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000118">
        <property type="entry name" value="protein delta homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000001">
        <property type="entry name" value="Urokinase-type plasminogen activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000391">
        <property type="entry name" value="Weary, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF40">
        <property type="entry name" value="HYALURONAN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 18" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 19" id="KW-0420">Kringle</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="102"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="179"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="21">
        <location>
            <begin position="222"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="22">
        <location>
            <begin position="338"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="583" mass="65823" checksum="B4663E6F507EB7D7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HG98</accession>
    <name>A0A452HG98_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HG98"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013854"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015861.1">
        <property type="protein sequence ID" value="ENSGAGP00000013854.1"/>
        <property type="gene ID" value="ENSGAGG00000010556.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000965">
        <property type="entry name" value="Neurotrophin receptor-interacting factor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000381">
        <property type="entry name" value="zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="206"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="214"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="245" mass="27183" checksum="C9D53EFAB6994E36" modified="2019-05-08" version="1">RRVPCGRSFLLRRLLDVHQLSHAGREPQVCAGCGAAFVTALQLREHKCGKVSRRFECPTCGKKVSSAARLRAHELLHDGGPSFECADCKKLFSTETSLQVHRRIHTGERPYPCPDCGKAFRQSTHLKDHRRLHTGEKPFKCEECGKAFTIAVRLAEHRRIHTGERPYHCADCGKAYRSFSNLWKHRKLHQQQRLQNEQEALAEAAAAAAMAAATQTPPGTVTTRPSASAAPHKPLPNPPETGGHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HII0</accession>
    <name>A0A452HII0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HII0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014712"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016820.1">
        <property type="protein sequence ID" value="ENSGAGP00000014712.1"/>
        <property type="gene ID" value="ENSGAGG00000011159.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19818">
        <property type="entry name" value="Bbox1_ZBBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.40">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding protein, ZZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038446">
        <property type="entry name" value="CEBP_ZZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037688">
        <property type="entry name" value="ZBBX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28634">
        <property type="entry name" value="ZINC FINGER B-BOX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28634:SF1">
        <property type="entry name" value="ZINC FINGER B-BOX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22586">
        <property type="entry name" value="ANCHR-like_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="4">
        <location>
            <begin position="12"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="211" mass="23677" checksum="14F1735EB9BEBDAF" modified="2019-05-08" version="1">MANVAIPGKPKMKGKACGQCETKNALLVCLECGEDYCGSCFARVHQKGALKLHRMIPLQAKTDVSVGKLEATHQFMKDFNPGESKKNHGQKKANSKNQLMSDISSSLLTSTVSTVPAGAVFENQNGGLLLYGTFDEEESAESFQEALTQWRNGNHEHKKEQNCDESVLSGISFKFCEVQTHLTILRKPVKIEFREESLNYMEKLWLKKHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIW9</accession>
    <name>A0A452HIW9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Flap endonuclease 1</fullName>
            <shortName evidence="17">FEN-1</shortName>
            <ecNumber evidence="17">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13 17">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300; can bind simultaneously to both PCNA and EP300. Interacts with DDX11; this interaction is direct and increases flap endonuclease activity of FEN1. Interacts with WDR4; regulating its endonuclease activity. Interacts with POLB.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with PCNA1 and PCNA2. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Nucleus</location>
            <location evidence="17">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Nucleus</location>
            <location evidence="17">Nucleoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Mitochondrion</location>
        </subcellularLocation>
        <text evidence="17">Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 17">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A452HIW9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014854"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016972.1">
        <property type="protein sequence ID" value="ENSGAGP00000014854.1"/>
        <property type="gene ID" value="ENSGAGG00000011248.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09907">
        <property type="entry name" value="H3TH_FEN1-Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000009">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000016">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF71">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 17" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2 17" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 17" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 17" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 17" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1 17" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="20">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="19">
        <location>
            <begin position="128"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="310"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="313"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="372" mass="41954" checksum="7EF760DBDE12CB4D" modified="2019-05-08" version="1">MGIARLADVIKEEAAEAVSSFPLRQYRDRVIALDASIAIYQFRTATPKLFNRNGENISHLQGLFYRTLHLLENGIKPVFVFDGQPPDLKQPLLARRAEAAGARRDAAESRRAENLPRPLKQDCETLLSYLGVPYVQAPAEAEATCAALVKSGKVWCTATEDMDALPFGSLRLIRHLNSKNNCEVEEFSLPDILQKLGMTQEQFVDLCILLGCDYCGKIWQLGPKKALKLLQQHGTIEQVLQNIDPQKHPLPANWDLEGARRLFLQPEVADPGRITLEWRDPDPEGLVRFLAHEKHMNEARVCRRLQRWQEAQPKPEEAQPKHQAPREGRKQRKLGEFFPASKHARPPEVRGVRARPPGTRGARGMAQGIILC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIX0</accession>
    <name>A0A452HIX0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kish</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in the early part of the secretory pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the KISH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HIX0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014848"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016965.1">
        <property type="protein sequence ID" value="ENSGAGP00000014848.1"/>
        <property type="gene ID" value="ENSGAGG00000011242.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051523">
        <property type="entry name" value="KISH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009653">
        <property type="entry name" value="Ksh1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13229">
        <property type="entry name" value="PROTEIN KISH-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06842">
        <property type="entry name" value="DUF1242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="99" mass="11326" checksum="42654F4BE216D935" modified="2019-05-08" version="1">MQITVSFTIWLREDSICILRFKAYNLGVSLNQLGNRCWSKESAIFNFQSLLTVILLLICTCAYIRSLAPSLLDKNKTGLLGIFWKCARIGKCVFFSPWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJC4</accession>
    <name>A0A452HJC4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJC4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015010"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017144.1">
        <property type="protein sequence ID" value="ENSGAGP00000015010.1"/>
        <property type="gene ID" value="ENSGAGG00000011341.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000015">
        <property type="entry name" value="Endothelial differentiation-related factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013729">
        <property type="entry name" value="MBF1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245:SF15">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1 MULTIPROTEIN BRIDGING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08523">
        <property type="entry name" value="MBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="6">
        <location>
            <begin position="106"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="173" mass="18578" checksum="42AFD2199FB0DF8D" modified="2019-05-08" version="1">RDSGLLSSNHSKPTEGVAGWAPYERRGGARRRRLRTGGAGRCHGGERLGHGHGAAQEGAERGPGQVQAGAAGQNKQHSITKNTAKLDRETEELHHDRVPLEVGKVIQQGRQSKGLTQKDLATKINEKPQVIADYECGRAIPNNQVLGKIERAIGLKLRGRDIGKPLEAGPKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HL14</accession>
    <name>A0A452HL14_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PHD finger protein 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HL14"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015638"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017861.1">
        <property type="protein sequence ID" value="ENSGAGP00000015638.1"/>
        <property type="gene ID" value="ENSGAGG00000011795.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051188">
        <property type="entry name" value="PHD-type_Zinc_Finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059102">
        <property type="entry name" value="PHD_PHF7/G2E3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420">
        <property type="entry name" value="PHD FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420:SF47">
        <property type="entry name" value="PHD FINGER PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26054">
        <property type="entry name" value="PHD_G2E3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="46"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="110"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="37133" checksum="8584CC2C622151D6" modified="2019-05-08" version="1">ADSNPDTYGQKCQRGKLCVHENCLVSSSLFPWGLIVLSRGWADLPTCCVCGCKGASIACQGRKCTKNFHFPCGSEKGCIYQFFGEFKSFCWKHRPVQRVRRRQQQAETICIVCLESMPGKPSYSAMVCPACKHAWFHRDCIQGHALRAALYHFRCPRCRDMGTFQREMFRMGIKIPDRDAAWEEDGAFSDQYHRHSHCDASCCLYVGGREQFADNGPWQLLLCNSCGSQGTHRRCSSLGATKGTWECEDCAEPTGAGKEPLERKDVLGPGFVWRELRADDCGHYPRWALGRRRRHAGRAWPKAHCSRGMSVTGLWIGAGKIPGWILDPKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HM21</accession>
    <name>A0A452HM21_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HM21"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015989"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018269.1">
        <property type="protein sequence ID" value="ENSGAGP00000015989.1"/>
        <property type="gene ID" value="ENSGAGG00000012025.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001833">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002434">
        <property type="entry name" value="Zinc finger protein 1168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000638">
        <property type="entry name" value="Zinc finger protein 184"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001823">
        <property type="entry name" value="Zinc finger protein 322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000052">
        <property type="entry name" value="zinc finger protein 546 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002134">
        <property type="entry name" value="Zinc finger protein 616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376:SF238">
        <property type="entry name" value="ZINC FINGER PROTEIN 850"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019071822" evidence="10">
        <location>
            <begin position="20"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="12">
        <location>
            <begin position="17"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="285"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="397"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="425"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="453"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="481"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="537"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="565"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="593"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="124"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="633" mass="72328" checksum="C658B804422E7904" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HN60</accession>
    <name>A0A452HN60_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ADRM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HN60"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016411"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018727.1">
        <property type="protein sequence ID" value="ENSGAGP00000016411.1"/>
        <property type="gene ID" value="ENSGAGG00000012288.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13314">
        <property type="entry name" value="PH_Rpn13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2020.20:FF:000001">
        <property type="entry name" value="Proteasomal ubiquitin receptor ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.70:FF:000001">
        <property type="entry name" value="Proteasomal ubiquitin receptor ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2020.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.70">
        <property type="entry name" value="Proteasomal ubiquitin receptor Rpn13/ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006773">
        <property type="entry name" value="Rpn13/ADRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044868">
        <property type="entry name" value="Rpn13/ADRM1_Pru"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038633">
        <property type="entry name" value="Rpn13/ADRM1_Pru_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032368">
        <property type="entry name" value="RPN13_DEUBAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038108">
        <property type="entry name" value="RPN13_DEUBAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225">
        <property type="entry name" value="ADHESION REGULATING MOLECULE 1 110 KDA CELL MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225:SF0">
        <property type="entry name" value="PROTEASOMAL UBIQUITIN RECEPTOR ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04683">
        <property type="entry name" value="Rpn13_ADRM1_Pru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16550">
        <property type="entry name" value="RPN13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51917">
        <property type="entry name" value="PRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pru" evidence="9">
        <location>
            <begin position="17"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="DEUBAD" evidence="8">
        <location>
            <begin position="280"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="193"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="193"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="389"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="409" mass="42420" checksum="A6738FE406F92F2E" modified="2019-05-08" version="1">MSSGALFPSLVPGSRGSSSKYLVEFRAGKMSLKGSTVTPDKRKGLVYIQQTDDSLIHFCWKDRTSGNVEDDLIIFPDDCEFKRVPQCTTGRVYVLKFKAGSKRLFFWMQEPKTDKDEEHCRKVNEYLNNPPIPGALGGSGSGGHELSALGGEGGLQSLLGNMSHNQLMQLIGPTGLGGLGGLGALTGPGLASLLGSGGPPTSSSSSSSRSQSAAVTPSSTTSSTRVTPAPSAPAAASASASATSPSPAPSSGNGTSTATSPTQPIQLSDLQNILATMNVPAGAGGQQVDLASVLTPEIMAPILANAEVQERLLPYLPSGESLPQTAEEIQNTLTSPQFQQALSMFSAALASGQLGPLMSQFGLPAEAVDAANKGDVEAFAKAMQNNVKSDQKEGESKEKKDEEEDMSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HP23</accession>
    <name>A0A452HP23_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HP23"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016740"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019111.1">
        <property type="protein sequence ID" value="ENSGAGP00000016740.1"/>
        <property type="gene ID" value="ENSGAGG00000012519.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000019112.1">
        <property type="protein sequence ID" value="ENSGAGP00000016741.1"/>
        <property type="gene ID" value="ENSGAGG00000012519.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17061">
        <property type="entry name" value="Ubl_IQUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037695">
        <property type="entry name" value="IQUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057887">
        <property type="entry name" value="IQUB_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21074">
        <property type="entry name" value="IQ AND UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21074:SF0">
        <property type="entry name" value="IQ AND UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25805">
        <property type="entry name" value="IQUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="231"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="469"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="889" mass="102796" checksum="603EC12A5BF005F6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HPK9</accession>
    <name>A0A452HPK9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HPK9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016939"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019333.1">
        <property type="protein sequence ID" value="ENSGAGP00000016939.1"/>
        <property type="gene ID" value="ENSGAGG00000012647.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="52"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="325" mass="35664" checksum="C708E80FB04528E4" modified="2019-05-08" version="1">MGPSAQPVCSSMKPGNQTPVTEFILEGLPNTRELPSLFFLLFLLLYLLTLMGNTLTLLTVLCNSRLHALPMYCFLGHLAFLDTCLSSVTVPKILAGLVGPSGRAISFGGCVVQLYTFHFLCSTECFLYTVMAYDRFLAVCQPLRYSVVMSRKTCLWLAAGTWLTGSIHAVIQAFLTFRLPYCGPNQVEYFLCDIPAMLKLACADTAANQVVILANIEAVGASCFLLICVSYAYIASAILKIRTVQGRQWAFSTCSAHLTLVLLYYGPPVFIYLHPSSSQASDGAVAVFYTAVTPVLNPFIYTLRNKEMKKALRKVVCGQTLSLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQG5</accession>
    <name>A0A452HQG5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HQG5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017271"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019701.1">
        <property type="protein sequence ID" value="ENSGAGP00000017271.1"/>
        <property type="gene ID" value="ENSGAGG00000012858.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="98" mass="10549" checksum="5A23DB10A9627315" modified="2019-05-08" version="1">MAPPEETRARLARAGQSHLLRFWAELAPAERAALLAALALLPAEELGAHCRRAAEARAAERGPPERSGRRMEPVPAEFLGSVSRSEPATLARWEAEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRJ2</accession>
    <name>A0A452HRJ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000017622"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020104.1">
        <property type="protein sequence ID" value="ENSGAGP00000017622.1"/>
        <property type="gene ID" value="ENSGAGG00000013086.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15257">
        <property type="entry name" value="7tmB2_GPR128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053066">
        <property type="entry name" value="ADGR_G7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047938">
        <property type="entry name" value="GPR128_7tmB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767:SF1">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="371"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="445"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="6">
        <location>
            <begin position="189"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="510" mass="57168" checksum="32608F8415E89B51" modified="2019-05-08" version="1">ADSLPALPPRCSGKWKEHGGREAQGCVLMLLQAPSPAAPIGRGSLFLANGRCWEWCLKQQQHTPLCLCSFMFQLLPGAEMCGSRAVFQYMCQLSLQKEQSLPNTVSLVYLCKVFVTSFSSCIPDPQYNKSEILLHDYACVFWDYNANDWDTKGCTKEGNKSELRCRCNHTTSFAVLMSFKINYKYAKPLEIVSDIGSGSSIAGLVITILFQILTRKTRKFSVTWMLVNLCTSMLIFNIIFISGIDNPNAKNNAAVSTNNTLLDSDRVDPPENASCTAVAVLLHYFLLATFMWTALNSAHLYLLLLKTLRPIPGHFTLIMSIIGWGVPAVVVAITLGATYRQGKQLNYRQEEFCWLAVLDDEGEVSIKKPMIGAFLLVVAVILLFNIIIFVRITVFVMWKENTNLTSNKKKSFTTKIFGTLSVAVMLGITWVLGYMMLINQEETNIVFSILFCIFNATQGLQICILYTFRSPIFKKKVTELFSFVSLPXCLTAGYINLLAFKDLLSFKIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUN7</accession>
    <name>A0A452HUN7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Voltage-gated potassium channel subunit beta-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">K(+) channel subunit beta-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Kv-beta-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Synapse</location>
            <location evidence="21">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HUN7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018816"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021448.1">
        <property type="protein sequence ID" value="ENSGAGP00000018816.1"/>
        <property type="gene ID" value="ENSGAGG00000013039.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044224">
        <property type="term" value="C:juxtaparanode region of axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098900">
        <property type="term" value="P:regulation of action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19141">
        <property type="entry name" value="Aldo_ket_red_shaker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000001">
        <property type="entry name" value="voltage-gated potassium channel subunit beta-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005983">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005401">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01293">
        <property type="entry name" value="Kv_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF1">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01579">
        <property type="entry name" value="KCNAB2CHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="9" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0770">Synapse</keyword>
    <keyword evidence="10" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="22">
        <location>
            <begin position="103"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00032589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="408" mass="45158" checksum="CE6583EFDF512C2C" modified="2019-05-08" version="1">MQVSFVCSEHSIKSRSADDRLSRQNASSPNLGTRSKFRAVAVVARSLGQLSVQNAPSSSESSVKQPGMKYRNLGKSGLRVSCLGLGKGFWAPAFYDGLSVNITNVSLQMAEQLMTLAYDNGINLFDTAEVYAAGKAEVVLGNIIKKKGWRRSSLVITTKIFWGGKAETERGLSRKHIIEGLKASLERLQLEYVDVVFANRPDPNTPMEETVRAMTHVINQGMAMYWGTSRWSSMEIMEAYSVARQFNLIPPICEQAEYHMFQREKVEVQLPELFHKIGVGAMTWSPLACGIVSGKYDGGIPPYSRASLKGYQWLKDKILSEEGRRQQAKLKELQAIAERLGCTLPQLAIAWCLRNEGVSSVLLGASSADQLMENIGAIQVLPKLSSSIIHEIDSILGNKPYSKKDYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HW77</accession>
    <name>A0A452HW77_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000019341"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022026.1">
        <property type="protein sequence ID" value="ENSGAGP00000019341.1"/>
        <property type="gene ID" value="ENSGAGG00000014269.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="6">
        <location>
            <begin position="584"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="488"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="615"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1845"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1940"/>
            <end position="2001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="489"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="501"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="616"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1940"/>
            <end position="1956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1957"/>
            <end position="1971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1992"/>
            <end position="2001"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2176" mass="241981" checksum="9180A45BA03A1320" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYJ2</accession>
    <name>A0A452HYJ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYJ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020241"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023063.1">
        <property type="protein sequence ID" value="ENSGAGP00000020241.1"/>
        <property type="gene ID" value="ENSGAGG00000014929.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051573">
        <property type="entry name" value="Ankyrin-SOCS_box_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136:SF18">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136">
        <property type="entry name" value="SOWAH (DROSOPHILA) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="114"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="146"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="179"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="213"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="287" mass="31756" checksum="98486E3EC1CB69C1" modified="2019-05-08" version="1">MSRETFVFTSSTLRALRLQRERLEWEDRRRALSRQCLTRRFPLATSSSLLPKPARRPRYCRDPAVHNILYTGDLQTIKNIFKDEAVANVIVETVSEELMWSAELGLWVLSPLKKHTSPLRIAAARGYRDCVKHLILQGAEVDAVVGGKAALHDSCIHQRAECTQLLLNYGANANVLSEEGLAPLHLCTSPETLQCAELLLEHGALVNLSTRDRRTTPLHVAAEHGLEAHLQLYLCYGAALAHRNREGETALNAACASADKPAEAELLLLGPPCPGSRAELLRPDPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I118</accession>
    <name>A0A452I118_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I118"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021161"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024102.1">
        <property type="protein sequence ID" value="ENSGAGP00000021161.1"/>
        <property type="gene ID" value="ENSGAGG00000015549.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18259">
        <property type="entry name" value="BTB_POZ_KLHL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF398">
        <property type="entry name" value="KELCH-LIKE PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="49"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="236" mass="26251" checksum="7F91EBF3619CAB8A" modified="2019-05-08" version="1">MLEPQEQERALPEDSIMVRNVDDFDFSLPSHPQAIMEGLRSLRSNPKLSDVTLLVGGHEFPCHRSILALCSHYFNAMFTGDFIESISAHVELKEVDPVVMETLIDFAYTGKITINQGNVEALIRTSNQLHFPTIRKVCSRYLRQQMDATNCLGICEFGENHGCPEVSSKAWAFLQENFEAVSAVYNCVWCPARTKGWAPSDFATALPGSKEPELPVTVFGDPPEPRAGPLLTLPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1G3</accession>
    <name>A0A452I1G3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I1G3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021337"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024303.1">
        <property type="protein sequence ID" value="ENSGAGP00000021337.1"/>
        <property type="gene ID" value="ENSGAGG00000015668.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45924">
        <property type="entry name" value="FI17866P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45924:SF4">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="1">
        <location>
            <begin position="103"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="315" mass="35102" checksum="D0A512D953E6014B" modified="2019-05-08" version="1">AAVSDSPAVGSIGQTSSWASSSALRWPDPGLLWHRLAVGGWDQGRDKAPGHAPAREGDALRVNQLHNSNNNASLGGWLGRRGSRSLSPFGPRASVGANHKLSYLERVVLELVESERTYVQDLRSIVEVSRPPPHTPSAERDMCALGRPCMTDSLFLPSELLQDLDSCDSNPVAVARCFVDRSQDFDIYTQYCNNYPSSVAALTECMRNKHQAKFFRERQEQIGHALPLGSYLLKPVQRILKYHLLLQEIAKHFDLEEAGYEVVEEAIDTMTCVAWYINDMKRRHEHAVRLQVSAGLGDSARQTGPWQAQLRELSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1G6</accession>
    <name>A0A452I1G6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>glycine(out) + chloride(out) + 2 Na(+)(out) = glycine(in) + chloride(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70691"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A9 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I1G6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021310"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024270.1">
        <property type="protein sequence ID" value="ENSGAGP00000021310.1"/>
        <property type="gene ID" value="ENSGAGG00000015071.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003028">
        <property type="entry name" value="Na/ntran_symport_glycine_GLY1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF263">
        <property type="entry name" value="SODIUM- AND CHLORIDE-DEPENDENT GLYCINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01204">
        <property type="entry name" value="GLY1TRNSPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0915">Sodium</keyword>
    <keyword evidence="7 14" id="KW-0769">Symport</keyword>
    <keyword evidence="5 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="351"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="404"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="433"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="492"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="534"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="566"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="607"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="641"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="25"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="718"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="731"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="504"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="741" mass="81634" checksum="287CD07D1F8A8A67" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2G6</accession>
    <name>A0A452I2G6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Formin like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I2G6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021691"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024710.1">
        <property type="protein sequence ID" value="ENSGAGP00000021691.1"/>
        <property type="gene ID" value="ENSGAGG00000015887.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000036">
        <property type="entry name" value="Formin-like protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000045">
        <property type="entry name" value="Formin-like protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043592">
        <property type="entry name" value="FMNL_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857:SF2">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="4">
        <location>
            <begin position="35"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="5">
        <location>
            <begin position="650"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="165"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="521"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="396"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="552"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="608"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="865" mass="96306" checksum="C8CD04FBD905C975" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2W5</accession>
    <name>A0A452I2W5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452I2W5"/>
    <dbReference type="SMR" id="A0A452I2W5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021852"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024892.1">
        <property type="protein sequence ID" value="ENSGAGP00000021851.1"/>
        <property type="gene ID" value="ENSGAGG00000016042.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000024893.1">
        <property type="protein sequence ID" value="ENSGAGP00000021852.1"/>
        <property type="gene ID" value="ENSGAGG00000016042.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23801">
        <property type="entry name" value="UBCc_UBE2L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000011">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="7">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="154" mass="17862" checksum="F5A30243BE3C9985" modified="2019-05-08" version="1">MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4L1</accession>
    <name>A0A452I4L1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000022453"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025580.1">
        <property type="protein sequence ID" value="ENSGAGP00000022453.1"/>
        <property type="gene ID" value="ENSGAGG00000016307.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097113">
        <property type="term" value="P:AMPA glutamate receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098970">
        <property type="term" value="P:postsynaptic neurotransmitter receptor diffusion trapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06723">
        <property type="entry name" value="PDZ1_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06724">
        <property type="entry name" value="PDZ2_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06795">
        <property type="entry name" value="PDZ3_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12030">
        <property type="entry name" value="SH3_DLG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000091">
        <property type="entry name" value="disks large homolog 1 isoform X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000047">
        <property type="entry name" value="Disks large homolog 2 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000002">
        <property type="entry name" value="Disks large homolog 4 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019583">
        <property type="entry name" value="DLG1-4_PDZ_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF33">
        <property type="entry name" value="DISKS LARGE HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10600">
        <property type="entry name" value="PDZ_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="40"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="130"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="283"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="398"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="511" mass="56451" checksum="A904F080811CE347" modified="2019-05-08" version="1">MCIRDRLXSLAVPPSPCWCPSLPACSPWRPTDRFRGCLLQGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNDVDVREVTHSTAVEALKEAGSIVRLYVMRRKALAEKVMEVKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNNVSLEDVMHEDAVAALKNTYDVVYLKVAKPTNMYLNDSYAPPDITTSYSQHLDNEISHQSYLGTDYPQAMTPTSPRRYSPIPKEMMGEEDIPREPRRIIIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNATHEQAAIALKNAGQTVTIIAQYKPDEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVVDASDDEWWQARRVHPDGEGDEIGFVPSKRRVERREWTRLKAKVAIVEGESFEEIYHKIKRIIEELSGPYIWIPARERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5M1</accession>
    <name>A0A452I5M1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lysosomal cobalamin transport escort protein LMBD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">LMBR1 domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LIMR family. LMBRD1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I5M1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022893"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026085.1">
        <property type="protein sequence ID" value="ENSGAGP00000022893.1"/>
        <property type="gene ID" value="ENSGAGG00000016782.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061462">
        <property type="term" value="P:protein localization to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050854">
        <property type="entry name" value="LMBD1_LysCbl_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16130:SF2">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORT ESCORT PROTEIN LMBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16130">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="9" id="KW-0170">Cobalt</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="312"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="488" mass="55567" checksum="16C981229B65B349" modified="2019-05-08" version="1">MAAASSELLTGWCLFGLSLLAILAFCWVYVRKYQSRRESEVVSTITAIFALAIALITSALLPVDIFLVSYMKNQNGTFKEWADANVKRQIEDTVLYGYYTLYSIVLFSVFLWIPFVYFYYEEKDEDDTSSCSQFQAALKYTLGFLMVCTVLLLIGAFVPLDVPQKRNSTEWEKVKFLFEELGSSHGLAALSFSISSLTLIGMLAAVTYTAYGMSALPLNLIKGTRSPTYERLENTEDIEEVEQRILRIKSKVWCLLHNISNFSIYIFPNYCSLDKALHSAGINSGFIILGTNLTNPLNMLLPVLQTVFPLDYILITTIIMYFIFTSMAGIRNMGIWFFWIRLYKIRRGKTRPQALLFLCMILLLIVLHTSYMIYSLAPQYVMYGSQKYLVQHNMTLVDGQPGNVTTYVPKVCDADAPKDQCTITRTYLFLHKFWFFSAAYYFGNWAFIAVFLIGLIVSCCKGKKSVIEGEVDEDDSDISDDEPCVYYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I689</accession>
    <name>A0A452I689_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquinone biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Membrane-associated protein that warps the membrane surface to access and bind aromatic isoprenes with high specificity, including ubiquinone (CoQ) isoprene intermediates and presents them directly to Coq7, therefore facilitating the Coq7-mediated hydroxylase step. Participates in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Heterodimer; two heterodimers of COQ7:COQ9 come together on the same side of the lipid pseudo-bilayer and form a curved tetramer with a hydrophobic surface suitable for membrane interaction. These two tetramers assemble into a soluble octamer with a pseudo-bilayer of lipids captured within. Interacts with COQ7; this interaction allows ubiquinone (CoQ) isoprene intermediates presentation to COQ7 and facilitates the COQ7-mediated hydroxylase step.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the COQ9 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000023124"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026350.1">
        <property type="protein sequence ID" value="ENSGAGP00000023124.1"/>
        <property type="gene ID" value="ENSGAGG00000016948.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.10:FF:000004">
        <property type="entry name" value="Ubiquinone biosynthesis protein COQ9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.10">
        <property type="entry name" value="Tetracycline Repressor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013718">
        <property type="entry name" value="COQ9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012762">
        <property type="entry name" value="Ubiq_biosynth_COQ9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02396">
        <property type="entry name" value="diverge_rpsU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08511">
        <property type="entry name" value="COQ9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 9" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="COQ9 C-terminal" evidence="10">
        <location>
            <begin position="77"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="144" mass="16617" checksum="38BD9BE41ED339BD" modified="2019-05-08" version="1">MFNNDGSELILHFVSQCNTELSNLLEEQQKLVQLGQAEKKKTDQFLKDAVEARLRMLIPYIEKWPQAVSILLLPHNIPSSLNLLTSMVDDMWHYAGDQSTDINWYTRRTMLAGIYNTTELVMMQDXKNTRDKIVALPMIHVLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6P4</accession>
    <name>A0A452I6P4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I6P4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023201"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026436.1">
        <property type="protein sequence ID" value="ENSGAGP00000023201.1"/>
        <property type="gene ID" value="ENSGAGG00000017010.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013302">
        <property type="entry name" value="Wnt10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF76">
        <property type="entry name" value="PROTEIN WNT-10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01893">
        <property type="entry name" value="WNT10PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019111174" description="Protein Wnt" evidence="12">
        <location>
            <begin position="18"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="374" mass="41642" checksum="BA542BBB22FC40F4" modified="2019-05-08" version="1">MGLAPLLALCLWSRVLSNDILGLKPPSDPVLTPNTVCLTLPGLSKRQMGLCVRSPDVTASALQGIQLAIQECQRQLWDQRWNCSSLEGVGQVLQDSAILKRGFRESAFAFSLLAAGVMHSVATACSLGRLQGCGCAWGEHGSQEKQRLKLSQLQALSRGKGLPPHLASLLGEPGPQDTWQWGGCGPDIRFGERFSRDFLDSREAPRDIQARMRIHNNWVGRQVVAENMKRKCKCHGTSGSCQFRTCWPVVPEFWLVGAVLRERMQHAAFISAHNRNSGVFGPRLGQRRLARELVYFEKSPDFCERDPALDSPGTRGRACNHTSPRTDGCASLCCGRGHNILRQTRRERCRCHFQWCCSVLCEQCRLSTWLSVCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7M9</accession>
    <name>A0A452I7M9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I7M9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023642"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026937.1">
        <property type="protein sequence ID" value="ENSGAGP00000023642.1"/>
        <property type="gene ID" value="ENSGAGG00000017325.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042382">
        <property type="term" value="C:paraspeckles"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12948">
        <property type="entry name" value="NOPS_PSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12587">
        <property type="entry name" value="RRM1_PSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12590">
        <property type="entry name" value="RRM2_PSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000043">
        <property type="entry name" value="paraspeckle component 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000126">
        <property type="entry name" value="paraspeckle component 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012975">
        <property type="entry name" value="NOPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034526">
        <property type="entry name" value="PSF_NOPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034525">
        <property type="entry name" value="PSF_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08075">
        <property type="entry name" value="NOPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="232"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="306"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="518"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="145"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="518"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="569"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="606"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="631"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="645" mass="69379" checksum="D9CA8DD2CCAA362C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7W0</accession>
    <name>A0A452I7W0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histone-lysine N-methyltransferase, H3 lysine-36 specific</fullName>
            <ecNumber evidence="20">2.1.1.357</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">H3-K36-HMTase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Nuclear receptor-binding SET domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-lysyl(36)-[histone H3] + 2 S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(36)-[histone H3] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60308"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:9787"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="2.1.1.357"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.357" evidence="20"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023721"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027020.1">
        <property type="protein sequence ID" value="ENSGAGP00000023721.1"/>
        <property type="gene ID" value="ENSGAGG00000016731.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140954">
        <property type="term" value="F:histone H3K36 dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016922">
        <property type="term" value="F:nuclear receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15648">
        <property type="entry name" value="PHD1_NSD1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15650">
        <property type="entry name" value="PHD2_NSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15653">
        <property type="entry name" value="PHD3_NSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20164">
        <property type="entry name" value="PWWP_NSD1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19210">
        <property type="entry name" value="SET_NSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000002">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000004">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000025">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000093">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000201">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000153">
        <property type="entry name" value="Histone-lysine N-methyltransferase NSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006560">
        <property type="entry name" value="AWS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041306">
        <property type="entry name" value="C5HCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055198">
        <property type="entry name" value="NSD_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059153">
        <property type="entry name" value="NSD_PHD-1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047426">
        <property type="entry name" value="PHD1_NSD1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047428">
        <property type="entry name" value="PHD2_NSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047429">
        <property type="entry name" value="PHD3_NSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055197">
        <property type="entry name" value="PHDvar_NSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047423">
        <property type="entry name" value="PWWP_NSD1_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050777">
        <property type="entry name" value="SET2_Histone-Lys_MeTrsfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047433">
        <property type="entry name" value="SET_NSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22884">
        <property type="entry name" value="SET DOMAIN PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17907">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17982">
        <property type="entry name" value="C5HCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23011">
        <property type="entry name" value="PHD-1st_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22908">
        <property type="entry name" value="PHD_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23004">
        <property type="entry name" value="PHDvar_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00570">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00508">
        <property type="entry name" value="PostSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51215">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0010">Activator</keyword>
    <keyword evidence="14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 23" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="25">
        <location>
            <begin position="357"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="25">
        <location>
            <begin position="521"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="PWWP" evidence="27">
        <location>
            <begin position="570"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="AWS" evidence="29">
        <location>
            <begin position="704"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="26">
        <location>
            <begin position="756"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="28">
        <location>
            <begin position="880"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="276"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1294"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00066810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00080495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00081785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1404" mass="157260" checksum="9E8A5B9E1F9586ED" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I892</accession>
    <name>A0A452I892_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">F-box/WD repeat-containing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">F-box and WD-40 domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Directly interacts with SKP1 and CUL1.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I892"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023867"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027184.1">
        <property type="protein sequence ID" value="ENSGAGP00000023867.1"/>
        <property type="gene ID" value="ENSGAGG00000017466.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22131">
        <property type="entry name" value="F-box_FBXW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000151">
        <property type="entry name" value="F-box/WD repeat-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042627">
        <property type="entry name" value="FBXW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44436">
        <property type="entry name" value="F-BOX/WD REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44436:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="10">
        <location>
            <begin position="54"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="144"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="222"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00079856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="454" mass="51736" checksum="46DE9D59B2AD05F6" modified="2019-05-08" version="1">MEKKDFEAWLDNISVTFLSLTDLQKNESLDHLISLSGAVQLRHLSNNLEILLKRDFLKLLPLELSFYLLKWLDPRTLLTCCLVSKQWNKVISACTEVWQTACKNLGWQIDDSVQDPLHWKKVYLKAILRMKQLKDHEAFETSSLIGHSARVYALYYKDGLLCTGSDDLSAKLWDVSTGQCIYGIQTHTCATVKFDEQKLVTGSFDNTIACWEWSSGAKIQHFRGHTGAVFSVDYNDELDVLVSGSADFTLKVWALSTGMCLNTLTGHTEWVTKVVLQKCKVKSLMHSPGDYILLSADKYEIKIWPIGREINCKCLKTLSVSEDRSISLQPRLHFDGKYIVCSSALGLYQWDFASYDILRVIKPPDFANLSLLGFGDIFALLFDNKYLYIMDLRTEKLISRWPLPEYRKSKRGSSFLAGEMSWLNGLDGQNDMGLVFATSMPDHSIHLVLWKEHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I952</accession>
    <name>A0A452I952_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Repulsive guidance molecule BMP co-receptor b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the repulsive guidance molecule (RGM) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I952"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024197"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027547.1">
        <property type="protein sequence ID" value="ENSGAGP00000024197.1"/>
        <property type="gene ID" value="ENSGAGG00000017692.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1000.10:FF:000001">
        <property type="entry name" value="Repulsive guidance molecule BMP co-receptor a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040287">
        <property type="entry name" value="RGM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009496">
        <property type="entry name" value="RGM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010536">
        <property type="entry name" value="RGM_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428:SF7">
        <property type="entry name" value="RGM DOMAIN FAMILY ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428">
        <property type="entry name" value="RGM DOMAIN FAMILY MEMBER DRAG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06534">
        <property type="entry name" value="RGM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06535">
        <property type="entry name" value="RGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019262002" description="Repulsive guidance molecule BMP co-receptor b" evidence="11">
        <location>
            <begin position="45"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Repulsive guidance molecule N-terminal" evidence="13">
        <location>
            <begin position="47"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Repulsive guidance molecule C-terminal" evidence="12">
        <location>
            <begin position="150"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="46821" checksum="603C82FCC91F486A" modified="2019-05-08" version="1">MVMGPGSLPPAAVERLRGPRRRPLLSVVVLLVSVCLAPPSAVHGQQCRIQRCNADYVAATSPSHVLPEETPLDMDYCIALRAYSVCTRKTAKSCRGDLVYHSAVFRIKELFTQHNCSSDGPTSSAKAPGTPDPLVSELCNYESRSGFQKKFAHCGLFGDPHLRTFKDEFQTCKVEGAWPLIDNQYLSVQVTNVPVVLGSSATATSKITLIFKSYQGCTEQKVYQATTEDLPLAFSDGTRTGGWQEGASSLRILEKPDAGQVEIQANYIGSTVIIRQVGRYLTFAIRVPEETLNLSEESTGLQLCLHGCPKNELIQEHRLSLGSSSPLWPSSRRAYTVEMATEQCRHILQVEDVYFQSCVFDLLTTGDPEFSMAAHGALEDLKALYPSRLKLHTVSNTINVATGAPGPGQPAASLVWCGLAFLKLFWCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9B9</accession>
    <name>A0A452I9B9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PLA2c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I9B9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024279"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027636.1">
        <property type="protein sequence ID" value="ENSGAGP00000024279.1"/>
        <property type="gene ID" value="ENSGAGG00000017744.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF39">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2 3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLA2c" evidence="5">
        <location>
            <begin position="5"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="297"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="491" mass="54897" checksum="6D28E935D047A13A" modified="2019-05-08" version="1">MKIFHKHKGEGKIRISHELSEGEKLGTSNRKVRVKKCLDSLGIPCNLDDVPNIAILGAGGGLRAMIALHGTLQELQKHGLLDTIMYLCGVSGSTWCMSYLYKNGDWTEKLQALEESMCARLSKYTWSVPKATKLLLEAAKDENYSLTEFWAYTVVYGMLHELDKGHLSEQQSASENGNNPYPIYAAVDERSFSKVTEYSPATWFEFTPHEASFLGYGASVPMEYFGSEYKNGELKKQKEEKNMSYLQGLWGSAIGSKTENEKVIKDIFFGFFKGEKESYSPSKESCPEGIVKGFPIPSTEPEAGDMEGSLHGEPAGASPGGFSPDTKNKPITENIKDGVGSVVDFARMWQKTTHGLLTWKWGTNNNFLYKCSALDSAELVKDKVISLVDAGIAINCAFPLVLRPERKVKLILSFDYGPFEPFKALKQTAKYCKENCIPFPEIDEKLLLEPDNPSDCYIFRGEGAPTVMHFPLFNRVNCPGNFRFAYFLKCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9X5</accession>
    <name>A0A452I9X5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I9X5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024332"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027696.1">
        <property type="protein sequence ID" value="ENSGAGP00000024332.1"/>
        <property type="gene ID" value="ENSGAGG00000017780.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000054">
        <property type="entry name" value="Nuclease-sensitive element-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050181">
        <property type="entry name" value="Cold_shock_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11544">
        <property type="entry name" value="COLD SHOCK DOMAIN CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="2">
        <location>
            <begin position="77"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="354" mass="38429" checksum="8794AEB1F065A206" modified="2019-05-08" version="1">MSEAAQTSPTAAAPPSPTGPPAAAAPASPAQDAKPKSPASPSAPAGSASGDAAPASDSSSSSAAPPADTEKKVLATKVLGTVKWFNVRNGYGFINRNDTKEDVFVHQTAIKKNNPRKYLRSVGDGETVEFDVVEGEKGAEAANVTGPDGVPVEGSRYAADRRRYRRGYYGRRRGPPRNYPGEEEEEEGSGSSEGLDPLITEGHIPGSRNQLRRPQYRQRFFPPYRAGLTFDRRSRVYSHADGIQGGDGEIKDGVPEGAQLHQQIHRNPSYRARYRRGPPRPRPASVVGEAENKENQHEANAMSQQPSRRGYRRPYNYRRRPRLPNAPSQDGNEIKVAETPPENPAPVTEQSSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB90</accession>
    <name>A0A452IB90_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IB90"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024780"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028198.1">
        <property type="protein sequence ID" value="ENSGAGP00000024780.1"/>
        <property type="gene ID" value="ENSGAGG00000018093.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="46" mass="5222" checksum="3B3945139FCFA7C2" modified="2019-05-08" version="1">MFNSPQIMQSPVGAGQEVGRSCIILEFKGRKIMVIICKHLNYKGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICQ0</accession>
    <name>A0A452ICQ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Elongator complex protein 3</fullName>
            <ecNumber evidence="15">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Catalytic tRNA acetyltransferase subunit of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15 16">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A452ICQ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025543"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029069.1">
        <property type="protein sequence ID" value="ENSGAGP00000025543.1"/>
        <property type="gene ID" value="ENSGAGG00000018662.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000003">
        <property type="entry name" value="Elongator complex protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="68"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="382"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="533" mass="60630" checksum="D6E6B7DA3804CA60" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFW4</accession>
    <name>A0A452IFW4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IFW4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026635"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030273.1">
        <property type="protein sequence ID" value="ENSGAGP00000026635.1"/>
        <property type="gene ID" value="ENSGAGG00000019396.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595:SF1">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="3"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="128" mass="14141" checksum="454B1E2F996DBE7C" modified="2019-05-08" version="1">ICKAMVKKGHTRDSVQCRVKVKDLRQAYQKTKEANRRSGAGPKTCHFYTELHAILGGSATTTPPLSVDSEVGLVISAKPEDSVDGEDEEEDGLAERTQHPNSQELFLTQMELPSQPLKPLAQTVKPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IG74</accession>
    <name>A0A452IG74_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IG74"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030549.1">
        <property type="protein sequence ID" value="ENSGAGP00000026874.1"/>
        <property type="gene ID" value="ENSGAGG00000019567.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019036412" evidence="1">
        <location>
            <begin position="20"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="61" mass="6845" checksum="6DE85AE3B355ACB7" modified="2019-05-08" version="1">FLFLVCLLGFDSEFKQLVAWVWWCTAVTPATWEAETGRSLELRGSGLQCAMPIRCPVPLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGP3</accession>
    <name>A0A452IGP3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IGP3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026939"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030612.1">
        <property type="protein sequence ID" value="ENSGAGP00000026934.1"/>
        <property type="gene ID" value="ENSGAGG00000019611.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000030614.1">
        <property type="protein sequence ID" value="ENSGAGP00000026936.1"/>
        <property type="gene ID" value="ENSGAGG00000019611.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000030617.1">
        <property type="protein sequence ID" value="ENSGAGP00000026939.1"/>
        <property type="gene ID" value="ENSGAGG00000019611.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00032">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08341">
        <property type="entry name" value="DED_Caspase_10_r1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08814">
        <property type="entry name" value="DED_Caspase_10_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000016">
        <property type="entry name" value="CASP8 and FADD-like apoptosis regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000038">
        <property type="entry name" value="Caspase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000014">
        <property type="entry name" value="Caspase 10, apoptosis-related cysteine peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035701">
        <property type="entry name" value="CASP10_DED2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033139">
        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016129">
        <property type="entry name" value="Caspase_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001875">
        <property type="entry name" value="DED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48169:SF7">
        <property type="entry name" value="CASPASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48169">
        <property type="entry name" value="DED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01335">
        <property type="entry name" value="DED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00031">
        <property type="entry name" value="DED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50168">
        <property type="entry name" value="DED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="DED" evidence="9">
        <location>
            <begin position="6"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="DED" evidence="9">
        <location>
            <begin position="101"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="11">
        <location>
            <begin position="293"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="10">
        <location>
            <begin position="442"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="541" mass="61728" checksum="831C612C45E19D88" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452II93</accession>
    <name>A0A452II93_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Carbonic anhydrase</fullName>
            <ecNumber evidence="3 8">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3 8"/>
    <dbReference type="AlphaFoldDB" id="A0A452II93"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027574"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031328.1">
        <property type="protein sequence ID" value="ENSGAGP00000027574.1"/>
        <property type="gene ID" value="ENSGAGG00000020035.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045177">
        <property type="term" value="C:apical part of cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015670">
        <property type="term" value="P:carbon dioxide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000001">
        <property type="entry name" value="Carbonic anhydrase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF120">
        <property type="entry name" value="CARBONIC ANHYDRASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="260" mass="29355" checksum="DB4F9C03A0D1E4E8" modified="2019-05-08" version="1">MSHHWGYASHNGPAHWHENFPIANGKRQSPIDINTESAQYDSSLKPLHFSYDPSTARNIVNNGHSFNVEFDDSADKSVLRGGALEGTYRLIQFHIHWGSCEGQGSEHTVDGVQYDAELHLVHWNTKYGRFGEAVKHPDGLTVVGVFLQVGSDRPGIQKIIDALDSIQTKGKQASFTDFDPKNLLPTSLDYWTYQGSLTTPPLLECVIWHVLREPIHVSSEQLCKLRGLCFSAENETPYHMMDNWRPCQPLKGREVRASFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKE2</accession>
    <name>A0A452IKE2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="40">Polyunsaturated fatty acid 5-lipoxygenase</fullName>
            <ecNumber evidence="39">1.13.11.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="41">Arachidonate 5-lipoxygenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="49 50">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="50" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="49" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="49" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>(12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + O2 = (5S)-hydroperoxy-(12S)-hydroxy-(6E,8Z,10E,14Z)-eicosatetraenoate</text>
            <dbReference type="Rhea" id="RHEA:62648"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:90680"/>
            <dbReference type="ChEBI" id="CHEBI:145837"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>(15S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + O2 = (5S)-hydroperoxy-(15S)-hydroxy-(6E,8Z,11Z,13E)-eicosatetraenoate</text>
            <dbReference type="Rhea" id="RHEA:48624"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57409"/>
            <dbReference type="ChEBI" id="CHEBI:131564"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:48625"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>(18R)-hydroxy-(5Z,8Z,11Z,14Z,16E)-eicosapentaenoate + O2 = (5S)-hydroperoxy-(18R)-hydroxy-(6E,8Z,11Z,14Z,16E)-eicosapentaenoate</text>
            <dbReference type="Rhea" id="RHEA:51968"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:90818"/>
            <dbReference type="ChEBI" id="CHEBI:132218"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:51969"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>(18S)-hydroxy-(5Z,8Z,11Z,14Z,16E)-eicosapentaenoate + O2 = (5S)-hydroperoxy-(18S)-hydroxy-(6E,8Z,11Z,14Z,16E)-eicosapentaenoate</text>
            <dbReference type="Rhea" id="RHEA:50204"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:132083"/>
            <dbReference type="ChEBI" id="CHEBI:132091"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:50205"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (14S)-hydroperoxy-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate</text>
            <dbReference type="Rhea" id="RHEA:41332"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:77016"/>
            <dbReference type="ChEBI" id="CHEBI:78048"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:41333"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (17S)-hydroperoxy-(4Z,7Z,10Z,13Z,15E,19Z)-docosahexaenoate</text>
            <dbReference type="Rhea" id="RHEA:50840"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:77016"/>
            <dbReference type="ChEBI" id="CHEBI:133795"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:50841"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (7S)-hydroperoxy-(4Z,8E,10Z,13Z,16Z,19Z)-docosahexaenoate</text>
            <dbReference type="Rhea" id="RHEA:64668"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:77016"/>
            <dbReference type="ChEBI" id="CHEBI:156049"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:64669"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>(5S)-hydroperoxy-(18R)-hydroxy-(6E,8Z,11Z,14Z,16E)-eicosapentaenoate = (5S,6S)-epoxy-(18R)-hydroxy-(7E,9E,11Z,14Z,16E)-eicosapentaenoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:50268"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:132218"/>
            <dbReference type="ChEBI" id="CHEBI:132219"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:50269"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(5S)-hydroperoxy-(18S)-hydroxy-(6E,8Z,11Z,14Z,16E)-eicosapentaenoate = (5S,6S)-epoxy-(18S)-hydroxy-(7E,9E,11Z,14Z,16E)-eicosapentaenoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:39107"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:132091"/>
            <dbReference type="ChEBI" id="CHEBI:134661"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:39108"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate = leukotriene A4 + H2O</text>
            <dbReference type="Rhea" id="RHEA:17961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57450"/>
            <dbReference type="ChEBI" id="CHEBI:57463"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:17962"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>(5S)-hydroperoxy-18-hydroxy-(7E,9E,11Z,14Z,16E)-eicosapentaenoate = (5S,6S)-epoxy-18-hydroxy-(7E,9E,11Z,14Z,16E)-eicosapentaenoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:50844"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:90826"/>
            <dbReference type="ChEBI" id="CHEBI:133812"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:50845"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>(5Z,8Z)-eicosadienoate + O2 = (5S)-hydroperoxy-(6E,8Z)-eicosadienoate</text>
            <dbReference type="Rhea" id="RHEA:62644"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:145835"/>
            <dbReference type="ChEBI" id="CHEBI:145836"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate</text>
            <dbReference type="Rhea" id="RHEA:10428"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:57444"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:10429"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate</text>
            <dbReference type="Rhea" id="RHEA:17485"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:57450"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:17486"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = (8S)-hydroperoxy-(5Z,9E,11Z,14Z)-eicosatetraenoate</text>
            <dbReference type="Rhea" id="RHEA:38675"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:75322"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:38676"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = leukotriene A4 + H2O</text>
            <dbReference type="Rhea" id="RHEA:32307"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:57463"/>
            <dbReference type="EC" id="1.13.11.34"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:32308"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + O2 = (5S)-hydroperoxy-(6E,8Z,11Z,14Z,17Z)-eicosapentaenoate</text>
            <dbReference type="Rhea" id="RHEA:62600"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:58562"/>
            <dbReference type="ChEBI" id="CHEBI:195399"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:62601"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>18-HEPE + O2 = (5S)-hydroperoxy-18-hydroxy-(7E,9E,11Z,14Z,16E)-eicosapentaenoate</text>
            <dbReference type="Rhea" id="RHEA:48860"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:90825"/>
            <dbReference type="ChEBI" id="CHEBI:90826"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:48861"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="42">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="42">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="37">Lipid metabolism; leukotriene A4 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="38">Homodimer. Interacts with ALOX5AP and LTC4S. Interacts with COTL1, the interaction is required for stability and efficient catalytic activity. Interacts with PIK3R1; this interaction bridges ALOX5 with CD40 after CD40 ligation in B cells and leads to the production of reactive oxygen species (ROS). Interacts (via PLAT domain) with DICER1 (via Dicer dsRNA-binding fold domain); this interaction enhances arachidonate 5-lipoxygenase activity and modifies the miRNA precursor processing activity of DICER1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="36">Nucleus intermembrane space</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 46">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="45">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.34" evidence="39"/>
    <dbReference type="AlphaFoldDB" id="A0A452IKE2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028361"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032219.1">
        <property type="protein sequence ID" value="ENSGAGP00000028361.1"/>
        <property type="gene ID" value="ENSGAGG00000020533.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005641">
        <property type="term" value="C:nuclear envelope lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004051">
        <property type="term" value="F:arachidonate 5-lipoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019370">
        <property type="term" value="P:leukotriene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01753">
        <property type="entry name" value="PLAT_LOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.245.10:FF:000001">
        <property type="entry name" value="Arachidonate 5-lipoxygenase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000002">
        <property type="entry name" value="Arachidonate 5-lipoxygenase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.60:FF:000003">
        <property type="entry name" value="Arachidonate 5-lipoxygenase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001885">
        <property type="entry name" value="LipOase_mml"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042062">
        <property type="entry name" value="PLAT_LOX_verte"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00467">
        <property type="entry name" value="MAMLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00711">
        <property type="entry name" value="LIPOXYGENASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 43" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 46" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 42 46" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0434">Leukotriene biosynthesis</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 42 46" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13 46" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="50" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="47">
        <location>
            <begin position="2"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="48">
        <location>
            <begin position="118"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="binding site" evidence="43">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="43">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="43">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="43">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="42">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="42">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="42">
        <location>
            <position position="551"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Essential for stabilizing binding to COTL1" evidence="44">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00050123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00050244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00050388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00050497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00050644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00050940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00051092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00051202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00051319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00051433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00051841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00051888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00051931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00052496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00052592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00052606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00060474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00060632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00061792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00066705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00071074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00078096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="45">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="46">
        <source>
            <dbReference type="RuleBase" id="RU003974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="47">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="48">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="49">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="50">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="674" mass="78552" checksum="BDFA9BED81137663" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILI2</accession>
    <name>A0A452ILI2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC45 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILI2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028696"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032593.1">
        <property type="protein sequence ID" value="ENSGAGP00000028696.1"/>
        <property type="gene ID" value="ENSGAGG00000020757.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003874">
        <property type="entry name" value="CDC45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10507">
        <property type="entry name" value="CDC45-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10507:SF0">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 45 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02724">
        <property type="entry name" value="CDC45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="583" mass="67117" checksum="EA65CED3EEA0637C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILZ0</accession>
    <name>A0A452ILZ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Myosin tail domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILZ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028870"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032798.1">
        <property type="protein sequence ID" value="ENSGAGP00000028870.1"/>
        <property type="gene ID" value="ENSGAGG00000020916.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349:SF6">
        <property type="entry name" value="MYOSIN-6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="5" id="KW-0518">Myosin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin tail" evidence="9">
        <location>
            <begin position="4"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="220" mass="25620" checksum="A58F2E11FF14189E" modified="2019-05-08" version="1">LLHSQNTSLINTRKKLEADMSQLQSEVDHTIQEARNAEEKAKKEITDVMALMVEELKKEQDTSAHLERMKKNLDQTVKDLQLRLNEAEQLALKGGKKQIQELKARVQELENELDAEQKRGIEAIKGVRKYKRRLKEITYQSEEDKKNVVRLQDLVDKLQLKVKAYKRQAEEEEQANTHLSWCHKAQHELEEAEERANITESQVNKLQAKSRNIGSKKSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IM99</accession>
    <name>A0A452IM99_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA-binding protein 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">RNA-binding motif protein 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the nuclear exosome targeting (NEXT) complex composed of MTREX, ZCCHC8, and RBM7 that directs a subset of non-coding short-lived RNAs for exosomal degradation. Interacts with ZCCHC8 and SF3B2/SAP145. Binds to MTREX through ZCCHC8. Interacts with YWHAE and YWHAZ; these interactions are stress-dependent and RBM7 phosphorylation dependent; release RNA from the NEXT complex and may affect RNA targeting to the nuclear RNA exosomome for degradation. Interacts with MEPCE and LARP7, the core subunits of 7SK snRNP; upon genotoxic stress this interaction is enhanced, triggering the release of inactive P-TEFb complex from the core and P-TEFb complex activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IM99"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029207"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033171.1">
        <property type="protein sequence ID" value="ENSGAGP00000029207.1"/>
        <property type="gene ID" value="ENSGAGG00000021127.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12592">
        <property type="entry name" value="RRM_RBM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000261">
        <property type="entry name" value="RNA-binding motif protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052285">
        <property type="entry name" value="NEXT_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034500">
        <property type="entry name" value="RBM7_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13798">
        <property type="entry name" value="RNA BINDING MOTIF RBM PROTEIN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13798:SF4">
        <property type="entry name" value="RNA-BINDING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="10"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="86"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="160"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="106"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="219"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00080345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="299" mass="34236" checksum="276E85F3FE66A0F7" modified="2019-05-08" version="1">MGAAAAEANRTLFVGNLDPRVTEELIFELFHQAGPVIKVKIPKDRDGKPKQFAFVNFKHEESVPYGMSLLNGIKLFGRPIKIQFRSGSSHASQDGNSSYSQHGTANTSPSSTPHSTPNSNSRYDRSTDNTLATGFTSVQMLQRSFSSPDNLQRQVMNSGTWQQSQYSGKYGSPHSDQSTYTSPGQQQSHSFNQSSGSQMQRRPDGSSAQRKNRLNSHPYMDRHFNREQRFGDYGSDHHYRGNRDEYSYEDRSPHDPGSDHYSRGNRDEYCYEDRSHDGWSQDYNRRENYRDGKWRPSWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452INA4</accession>
    <name>A0A452INA4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452INA4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029375"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033356.1">
        <property type="protein sequence ID" value="ENSGAGP00000029375.1"/>
        <property type="gene ID" value="ENSGAGG00000021237.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070915">
        <property type="term" value="F:lysophosphatidic acid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15155">
        <property type="entry name" value="7tmA_LPAR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000017">
        <property type="entry name" value="lysophosphatidic acid receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF41">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01067">
        <property type="entry name" value="P2Y5ORPHANR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="35"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="349" mass="39354" checksum="1D7F683A1AE4CB36" modified="2019-05-08" version="1">MGNHSNNHTCLTDDSFKYNLYGAVYSVVFILGLITNCASLFVFCCRMKMRSETTIFMTNLAVSDLLFVFTLPFKIFYNFNRHWPFGDTLCKISGTAFLTNIYGSMLFLTCISVNRFLAIVYPFRSRTIRTRRNSAIVCAGVWILVLSGGILASLFSTTNVSNTSTTCFEGFSKIVWKTYLSKITIFIEVVGFIIPLLINLTCSSLVLRTLRKPATLSQIGTNKMKVLKMIIVHVAIFVVCFVPYNSILFLYALVRSQAIANCSLERFARTMYPITLCIATMNCCFDPFIYYFTSESFQKSFNIHPQIQMDSISKTETPLTMKTALPAIQEEVNDQAITNGGDPIAESHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRE5</accession>
    <name>A0A452IRE5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gypsy retrotransposon integrase-like protein 1</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452IRE5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030554"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034672.1">
        <property type="protein sequence ID" value="ENSGAGP00000030554.1"/>
        <property type="gene ID" value="ENSGAGG00000022002.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000003">
        <property type="entry name" value="Protein CBG25708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000026">
        <property type="entry name" value="Transposon Ty3-G Gag-Pol polyprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="10">
        <location>
            <begin position="285"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="831" mass="92883" checksum="04ADCB9F20ABBBAB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IX90</accession>
    <name>A0A452IX90_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IX90"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032668"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037025.1">
        <property type="protein sequence ID" value="ENSGAGP00000032668.1"/>
        <property type="gene ID" value="ENSGAGG00000023353.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097060">
        <property type="term" value="C:synaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027947">
        <property type="entry name" value="TMEM240"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28666">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28666:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15207">
        <property type="entry name" value="TMEM240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="149" mass="17872" checksum="783606FC9091B356" modified="2019-05-08" version="1">MDMNALLDRFHNYILPHLRGEDRVCHCNCGRHHVHYVIPYDGDQSVVDSSENYFVTDNVTKQEIDLMLGLLLGFCISWFLVWMDGVLHYAVRAWRTSRRYDNSWSWIPKFCNLKEFRKRQHRQYEEATGNMVHIKQKLYHNGHPSPRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IY57</accession>
    <name>A0A452IY57_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCA7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IY57"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033010"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037425.1">
        <property type="protein sequence ID" value="ENSGAGP00000033010.1"/>
        <property type="gene ID" value="ENSGAGG00000023580.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052237">
        <property type="entry name" value="Ataxin-7-like_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013243">
        <property type="entry name" value="SCA7_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15117">
        <property type="entry name" value="ATAXIN 7 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15117:SF9">
        <property type="entry name" value="ATAXIN-7-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08313">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51505">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCA7" evidence="2">
        <location>
            <begin position="207"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="527"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="540"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="571"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="634"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="695" mass="74958" checksum="35CAA5662DAC9DD2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYW8</accession>
    <name>A0A452IYW8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger MYND domain-containing protein 10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Plays a role in axonemal structure organization and motility. Involved in axonemal pre-assembly of inner and outer dynein arms (IDA and ODA, respectively) for proper axoneme building for cilia motility. May act by indirectly regulating transcription of dynein proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriolar satellite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Dynein axonemal particle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ZMYND10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYW8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033257"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037684.1">
        <property type="protein sequence ID" value="ENSGAGP00000033257.1"/>
        <property type="gene ID" value="ENSGAGG00000023706.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2220:FF:000009">
        <property type="entry name" value="Zinc finger MYND domain-containing protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052298">
        <property type="entry name" value="ZMYND10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13244">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13244:SF7">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="15">
        <location>
            <begin position="400"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="454" mass="52267" checksum="25444A37F90FF103" modified="2019-05-08" version="1">MAEMAAVLLPGEAEALVQALRSGPLRNTGDRGWLRQHEAIEKLNMQAILSASAGQEQLLIELLVNYAKIPTLIEELISVEIWKHKVFPVLCQLEDFKPKSTFPIYLVLYHEASIVNLLETVFFHKEICESAEDTILDLIDYSYRKLTLLAAWSTNREFPTHEKLVPEDLTNASSMQELQKQAEMMEFKISLRALSVLRFITDQVDSLPLSALTRMLNTHNVPCLLIELLEHCPWSCREAGKLKKFENGMWHVVPPEDHVKMTKLDGQVWIALYNLLLSPECQRKYNLDGFNKSQLLKLRAFLTDVLIDQLPNLVEMQRFLSHLAVTEPVPPKKDLILEQVPVIRDHIIRENSGKWQAIAKHQVKHMFSPSEDELKDLACRWAQTYNLDVMEALVPEKPKCGLCGSEATKRCSRCQNEWYCKRECQVQHWQKHKKACNLMADALKKIKEDLKVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZC3</accession>
    <name>A0A452IZC3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GRB2-associated and regulator of MAPK1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GAREM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZC3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033444"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037887.1">
        <property type="protein sequence ID" value="ENSGAGP00000033444.1"/>
        <property type="gene ID" value="ENSGAGG00000023832.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070064">
        <property type="term" value="F:proline-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071364">
        <property type="term" value="P:cellular response to epidermal growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007173">
        <property type="term" value="P:epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09525">
        <property type="entry name" value="SAM_GAREM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000042">
        <property type="entry name" value="GRB2-associated and regulator of MAPK protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025946">
        <property type="entry name" value="CABIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052281">
        <property type="entry name" value="GAREM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14454:SF6">
        <property type="entry name" value="GRB2-ASSOCIATED AND REGULATOR OF MAPK PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14454">
        <property type="entry name" value="GRB2-ASSOCIATED AND REGULATOR OF MAPK PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12736">
        <property type="entry name" value="CABIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0497">Mitogen</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CABIT" evidence="6">
        <location>
            <begin position="32"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="506"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="532"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="560"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00077585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="883" mass="98858" checksum="8905C70BA3A201F7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZV6</accession>
    <name>A0A452IZV6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZV6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033638"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038103.1">
        <property type="protein sequence ID" value="ENSGAGP00000033638.1"/>
        <property type="gene ID" value="ENSGAGG00000023947.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004940">
        <property type="term" value="F:beta1-adrenergic receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015052">
        <property type="term" value="F:beta3-adrenergic receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051379">
        <property type="term" value="F:epinephrine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002025">
        <property type="term" value="P:norepinephrine-epinephrine-mediated vasodilation involved in regulation of systemic arterial blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045823">
        <property type="term" value="P:positive regulation of heart contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15959">
        <property type="entry name" value="7tmA_Beta3_AR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000057">
        <property type="entry name" value="Beta-1 adrenergic receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002233">
        <property type="entry name" value="ADR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000507">
        <property type="entry name" value="ADRB1_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF3">
        <property type="entry name" value="BETA-3 ADRENERGIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01103">
        <property type="entry name" value="ADRENERGICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00561">
        <property type="entry name" value="ADRENRGCB1AR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="45"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="364"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="371"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="445" mass="48973" checksum="59A8699741FC81C1" modified="2019-05-08" version="1">MAPWPESNATSWGDATTNCTGAGSLSQQWAVGAALSLTVLVIAAGNLLVILAIAKTPRLQTMTNVFVTSLACADLIMGLLVVPPGATIVLSGHWPYGTTVCELWTSLDVLCVTASIETLCAIAVDRYLAITSPLQYEALVTKGRARAVVCLVWAISAFVSFLPIMNHWWRDGADEEALRCYQNPRCCDFVTNMPYAILSSIISFYVPLLVMLFVYARVFAVASRQISLIERDKVRFLQEPPSPGAKGPRRRRPSRLMAMREHKALKTLGIIMGTFTLCWLPFFVANIVKVFYRSLIPGQVFLFLNWLGYVNSAFNPIIYCRSPDFRSAFKKLLCCPRSADRRLHAVSRDLRRCPCAFASQEASPADSKPLEQGQQPGQAAEEDGGTSSSSSRSSSRTEENNLSYRQSNGHGNGQRPLREAQLQGTQITLCLQLEEFTCNEAKAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0C4</accession>
    <name>A0A452J0C4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G protein-coupled receptor kinase</fullName>
            <ecNumber evidence="11">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[G-protein-coupled receptor] + ATP = [G-protein-coupled receptor]-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12008"/>
            <dbReference type="Rhea" id="RHEA-COMP:11260"/>
            <dbReference type="Rhea" id="RHEA-COMP:11261"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A452J0C4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033807"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038295.1">
        <property type="protein sequence ID" value="ENSGAGP00000033807.1"/>
        <property type="gene ID" value="ENSGAGG00000024060.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004703">
        <property type="term" value="F:G protein-coupled receptor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05605">
        <property type="entry name" value="STKc_GRK4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000009">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000074">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001100">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000239">
        <property type="entry name" value="GPCR_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF26">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00717">
        <property type="entry name" value="GPCRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RGS" evidence="13">
        <location>
            <begin position="84"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="211"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="474"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600239-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="633" mass="72890" checksum="6D40B7789CC05217" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J177</accession>
    <name>A0A452J177_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 6">Anaphase-promoting complex subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cyclosome subunit 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the APC4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J177"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034131"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038648.1">
        <property type="protein sequence ID" value="ENSGAGP00000034131.1"/>
        <property type="gene ID" value="ENSGAGG00000024170.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030071">
        <property type="term" value="P:regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024789">
        <property type="entry name" value="APC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056358">
        <property type="entry name" value="APC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024790">
        <property type="entry name" value="APC4_long_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017169">
        <property type="entry name" value="APC4_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260">
        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX SUBUNIT 4 APC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12896">
        <property type="entry name" value="ANAPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23405">
        <property type="entry name" value="WD40_APC4_C-half"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037303">
        <property type="entry name" value="APC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 6" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="9">
        <location>
            <begin position="19"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4 long" evidence="10">
        <location>
            <begin position="198"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4 C-terminal half WD40" evidence="11">
        <location>
            <begin position="546"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="719"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="719"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="774" mass="87684" checksum="3A7E43D47DF137CF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4E4</accession>
    <name>A0A452J4E4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bcl-2/adenovirus E1B 19 kDa-interacting protein 2-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May be a bridge molecule between BCL2 and ARHGAP1/CDC42 in promoting cell death.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer. Interacts with BCL2, ARHGAP1, MIF and GFER.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J4E4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035333"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040007.1">
        <property type="protein sequence ID" value="ENSGAGP00000035333.1"/>
        <property type="gene ID" value="ENSGAGG00000025119.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000012">
        <property type="entry name" value="bcl-2/adenovirus E1B 19 kDa-interacting protein 2-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022181">
        <property type="entry name" value="Bcl2-/adenovirus-E1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF21">
        <property type="entry name" value="BCL-2_ADENOVIRUS E1B 19 KDA-INTERACTING PROTEIN 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12496">
        <property type="entry name" value="BNIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="6">
        <location>
            <begin position="194"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00055513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00065286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="362" mass="41530" checksum="862B3E2340330599" modified="2019-05-08" version="1">MGSYADCDEKLQPQRDQKDLEKQDLAEQEEGAVSIQDMELKEEWQDEEFPGPLPEEVSEEDLEDSSGEPNPVAPNTLELCGKRHMKKRLPAPDLSLSLDRAEGSVQSGDYPECTPDGSLDINVDELETPSESELLDGPESGHDFEWEDELPRARCLDSSLLEEKLGEGRIVDVEDKDGRKWRIFLTDEREQKVDLSVVEPYKRVISHGGYYGEGLNAIILFASCYLPHSSIPDYQYVMEHLFRYIIGTLEVMVAESYILVCLNGATLRSQIPSFGWIKQCYQTIDRRLRKNLKAMIIVHPTWYIKALMVVFRPFLSSKFGRKVQFVDSLWELSQQIPLEQVHIPDCIRQLDQSWNGSRDTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4Q2</accession>
    <name>A0A452J4Q2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J4Q2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035311"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039985.1">
        <property type="protein sequence ID" value="ENSGAGP00000035311.1"/>
        <property type="gene ID" value="ENSGAGG00000025100.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000039987.1">
        <property type="protein sequence ID" value="ENSGAGP00000035313.1"/>
        <property type="gene ID" value="ENSGAGG00000025100.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22136">
        <property type="entry name" value="F-box_FBXW10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051075">
        <property type="entry name" value="SCF_subunit_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872:SF7">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING PROTEIN 10B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872">
        <property type="entry name" value="UBIQUITIN LIGASE SPECIFICITY FACTOR/HREP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="302"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="472"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="514"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="593"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="816"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="829"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1144" mass="129188" checksum="449BD4680AB6165D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4T8</accession>
    <name>A0A452J4T8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000035475"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040170.1">
        <property type="protein sequence ID" value="ENSGAGP00000035475.1"/>
        <property type="gene ID" value="ENSGAGG00000025213.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="167"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="364" mass="38320" checksum="0A8DC95CCE560781" modified="2019-05-08" version="1">MIAGQVSKPLLSLRSEMNAELRGEDKAATSDNELNEPLLAPVESNDSEDTPSKLFGARGNPVLSDTSTADQHQPSQSHPAFPVGPQPLLTAQQLASAVAGVMPGGTPALNQPILIPFNMAGQLGGQQGLVLTLPTANLTNIQGLVAAAAAGGIMTLPLQNLQATSSLNSQLQQLQHLQQLQQQQQQQQQQGQQQQQAQQTQQAQQPQTPQQQQQPPQQQNQTQSQSQTQQPPAQQASNSPQKPNQSPGHGLPSPLTSPNPLQVQCNSXPQKPNQSPGHGLPSPLTSPNPLQIIGTIPLMPNPVPSNQAAGGAQGLQVQPITPQLLTNAQGQIIATVIGNQILPVINTQGITLSPLKPGQQVNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GF51</accession>
    <name>A0A452GF51_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GF51"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000131"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000153.1">
        <property type="protein sequence ID" value="ENSGAGP00000000131.1"/>
        <property type="gene ID" value="ENSGAGG00000000101.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008116">
        <property type="entry name" value="SSDP_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12610">
        <property type="entry name" value="SINGLE STRANDED DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12610:SF30">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01743">
        <property type="entry name" value="SSDNABINDING"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="117" mass="12415" checksum="18548AFBE3F3D001" modified="2019-05-08" version="1">MYAKGKGSAVPSDSQAREKLALYVYEYLLHVGAQKSAQTFLSEVRLGWPLAGGRLRTPGRAHVQGSSLSFPRSLTGLGVEPGLVLGCSDGLGLVARSSHPHLLSCPRPVLLVCTMTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GF96</accession>
    <name>A0A452GF96_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GF96"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000232"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000264.1">
        <property type="protein sequence ID" value="ENSGAGP00000000232.1"/>
        <property type="gene ID" value="ENSGAGG00000000148.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15230">
        <property type="entry name" value="7tmA_OR5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="303" mass="34165" checksum="3365D29EF10CA19D" modified="2019-05-08" version="1">MAEGNHTMVTDFIFQGFMDHPEHQVPLFVSFLMIYVITLAGNLGMIMLIRFNSQLHTPMYYFLCNLSLADVGNSSVVAPRLLMTFVIQAKPISLAACSAQFFFVCNFLTNEACLLAVMAYDRFIAICNPLLYSIVMSKRLCVLLVVASYICGFVNAVVQTPFIFTLSFCDSNVINHFFCDIPPILKLSCSDTHNANMVHFTLSSIVVMTTILIVLFSYMYILIAILKIHSAKGRYKTFSTCASHLTAVTIFYGTVIFMYLRPSSGYTTDPDKIISVFYILIIPMLNPLIYSLRNKEVKDLMLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIM3</accession>
    <name>A0A452GIM3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP complex mu/sigma subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GIM3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001440"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001630.1">
        <property type="protein sequence ID" value="ENSGAGP00000001440.1"/>
        <property type="gene ID" value="ENSGAGG00000001155.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14834">
        <property type="entry name" value="AP3_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000001">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027155">
        <property type="entry name" value="APS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="10">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="193" mass="21807" checksum="30DE2896638578E7" modified="2019-05-08" version="1">MIKAILIFNNHGKPRLSKFYQRYSEDTQQQIIRETFHLVSKRDENVCNFLEGGLLIGGSDNKLIYRHYATLYFVFCVDSSESELGILDLIQVFVETLDKCFENVCELDLIFHVDKVHNILSEMVMGGMVLETNMNEIVTQIDAQNKLEKSEAGLAGAPARAVSAVKNMNLPEIPRNINIGDISIKVPNLPSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIU3</accession>
    <name>A0A452GIU3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Vitamin K-dependent protein C</fullName>
            <ecNumber evidence="22">3.4.21.69</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Anticoagulant protein C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Autoprothrombin IIA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Blood coagulation factor XIV</fullName>
        </alternativeName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Degradation of blood coagulation factors Va and VIIIa.</text>
            <dbReference type="EC" id="3.4.21.69"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="28">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.69" evidence="22"/>
    <dbReference type="AlphaFoldDB" id="A0A452GIU3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001595"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001814.1">
        <property type="protein sequence ID" value="ENSGAGP00000001595.1"/>
        <property type="gene ID" value="ENSGAGG00000001280.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030195">
        <property type="term" value="P:negative regulation of blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012224">
        <property type="entry name" value="Pept_S1A_FX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF0">
        <property type="entry name" value="VITAMIN K-DEPENDENT PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001143">
        <property type="entry name" value="Factor_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="18 28" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 28" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="11 29" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 29" id="KW-0645">Protease</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="13 29" id="KW-0720">Serine protease</keyword>
    <keyword evidence="17" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Gla" evidence="32">
        <location>
            <begin position="29"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="30">
        <location>
            <begin position="75"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="31">
        <location>
            <begin position="182"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="28">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="28">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00037553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00038995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00040219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00041306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00042403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00042906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR001143-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="424" mass="47852" checksum="98773627B5B16DB9" modified="2019-05-08" version="1">MSPMLVLFSRLQVFYSSKDANQILKIQKRANSFLEELKPGSVERECNEEQCDLEEAHEIFETREATLNFWTKYVDGDQCLSNPCFNGTCLDNIGRFNCICNQGWEGRLCQHEANYTNCSTYNGGCEHFCHEDPKQSQHRYCSCASGYQLMDDHSKCEPVVEFPCGRVKADRMEFKSGIQIRLIDGKMGRKGDSPWQTMLLDGRGKFMCGGVLIHPSWVLTAAHCIEKKGRFKVKLGEYHRLRNEETEQTIVVDKLVSHENYSKKTSDNDIALLHLAQPMIFNKFVLPVCLPTKQLAEQELMANGKQMVVTGWGSKSGDSSNYSAVLSYIEIPMVPQNDCAQAMRHDISENMLCAGIIGDKQDACGGDSGGPMITKFKNTWFLVGLVSWGEGCGKLEKFGVYTKVSQYLEWIHQQIKTMDVSSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIU6</accession>
    <name>A0A452GIU6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GIU6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001589"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001808.1">
        <property type="protein sequence ID" value="ENSGAGP00000001589.1"/>
        <property type="gene ID" value="ENSGAGG00000001275.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="59" mass="6540" checksum="EE0DA059BE004BBE" modified="2019-05-08" version="1">THTVTCHSPPFPLIMTLGYVLELRVACKGISDRDALSKPDPCVILKMQSHGQWFEVSGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJ13</accession>
    <name>A0A452GJ13_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GJ13"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001648"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001873.1">
        <property type="protein sequence ID" value="ENSGAGP00000001648.1"/>
        <property type="gene ID" value="ENSGAGG00000001320.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049218">
        <property type="entry name" value="DUF5575_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049217">
        <property type="entry name" value="DUF5575_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040854">
        <property type="entry name" value="ZSWIM9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048315">
        <property type="entry name" value="ZSWIM9_RNaseH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47086">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47086:SF5">
        <property type="entry name" value="SWIM-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17738">
        <property type="entry name" value="DUF5575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20784">
        <property type="entry name" value="DUF5575_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20783">
        <property type="entry name" value="DUF5575_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="482"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="534" mass="59899" checksum="AFFB657BDEA828C0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJ14</accession>
    <name>A0A452GJ14_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GJ14"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001602"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001822.1">
        <property type="protein sequence ID" value="ENSGAGP00000001602.1"/>
        <property type="gene ID" value="ENSGAGG00000001285.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="84" mass="9766" checksum="12BE9A64A53341D5" modified="2019-05-08" version="1">MPGKSFFVHPSSAKSLEELSFLMQIEQYLEDVPEEIERYILDGKPKTAIEVRETGAKWVEVAEKKKTTSSWSEYQRGYKPTMLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJH6</accession>
    <name>A0A452GJH6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the clarin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GJH6"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002074.1">
        <property type="protein sequence ID" value="ENSGAGP00000001823.1"/>
        <property type="gene ID" value="ENSGAGG00000001460.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026748">
        <property type="entry name" value="Clarin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31548">
        <property type="entry name" value="CLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31548:SF5">
        <property type="entry name" value="CLARIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25807">
        <property type="entry name" value="Clarin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="182" mass="20427" checksum="1E4CB2F8DF31DB62" modified="2019-05-08" version="1">QGENWYGLGKGTGTRTQRLDGERFELQTSGDRVLKKIYALNICFLPVFPHLVKKLNASLHVMILIFLCAAISFALVSFGFCILNAIKVPYRAIKGPAGICLWNLLAASVKLHRLTERIANFQENVFQFVILEEQYESSIWLCVASATSHVVNLLLIAVSGVRFPEFRTKTEEATVTAEDIMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKD1</accession>
    <name>A0A452GKD1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GKD1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002194"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002503.1">
        <property type="protein sequence ID" value="ENSGAGP00000002194.1"/>
        <property type="gene ID" value="ENSGAGG00000001771.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="19"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="177" mass="20067" checksum="F5071718AA827224" modified="2019-05-08" version="1">WYQKWGLCPNVDVSLETFRQQFQSLAYNAGAHPRLVAQELRDLCQRWLQPDRQNPEELMQQIVLEQFLHILPSRGRAWVLRRVNTRTKPRTPPRGQGPRYRCPKSPPRQTPVGPETGASRTQRSPATSQKIPDGWSGPRSVTVPSGPSETAFAANPSCTALVFPEDSSSRPTPRKWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKK3</accession>
    <name>A0A452GKK3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSGAGT00000002481.1">
        <property type="protein sequence ID" value="ENSGAGP00000002174.1"/>
        <property type="gene ID" value="ENSGAGG00000001757.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001297">
        <property type="entry name" value="Zinc finger and SCAN domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039938">
        <property type="entry name" value="Sp4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14947">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14947:SF24">
        <property type="entry name" value="ZINC FINGER PROTEIN 781-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="295" mass="32431" checksum="0E2A8B6197F2B630" modified="2019-05-08" version="1">MTCEKEEQNSQQKNVQQLDKHRALLQRSKSNVSRSHEEEKSCEIQHRPEREQGNQPREKLGKRISSQGTQKDLKETTTKQEIIIGKRKNTCTECGKNICDYSALLNHQRIHTGDWPYECCERGKTSLTDHPFLFIGESTQGTGPMNAVSVGKGSLKDQPFLYIRESTQGKGPLNAVSVGKDSLAAQPFLIIRESTQRTGPMNAVSVGKFSLTDQAFVDIRESTQGRCPTNAVSVGKPSLAAQPFLSIRESTQGTGPMNAVSVGKPSLAAQPFLSIRESTQGTGPMXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLS2</accession>
    <name>A0A452GLS2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GLS2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002757"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003156.1">
        <property type="protein sequence ID" value="ENSGAGP00000002757.1"/>
        <property type="gene ID" value="ENSGAGG00000002212.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06880">
        <property type="entry name" value="PX_SNX22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052467">
        <property type="entry name" value="Sorting_nexin_PX-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15813:SF8">
        <property type="entry name" value="SORTING NEXIN-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15813">
        <property type="entry name" value="SORTING NEXIN-22 AND 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="185" mass="21077" checksum="38E5444F1BC5BF60" modified="2019-05-08" version="1">MIAVSIPAVGPGAEAEPSPEKTHTVFTVEVLCNGRKHTIEKRYSEFHALHKRIKKMCKVPDFPPKRVPNWMSKVQEQRRQGLEVYIQGILYYNKELPKELLDFLKLRHFQQDAKASSLDHGFLLSHRPVMIFHKDPYVLPSSTELLPDIVLSGVLRGFYRPASCCWEKAALKCLAGPHMLSQQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GM64</accession>
    <name>A0A452GM64_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc finger protein 135</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GM64"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002775"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003175.1">
        <property type="protein sequence ID" value="ENSGAGP00000002775.1"/>
        <property type="gene ID" value="ENSGAGG00000002214.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003095">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000029">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001772">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000258">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000218">
        <property type="entry name" value="Zinc finger protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000352">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000953">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF68">
        <property type="entry name" value="ZINC FINGER PROTEIN 358-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="14"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="424"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="452"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="480"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="508"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="536"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="564"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="592"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="620"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="647" mass="73920" checksum="2CC0DFC14C365C27" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPB7</accession>
    <name>A0A452GPB7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Monocarboxylate transporter 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Solute carrier family 16 member 9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Extracellular pH-and Na(+)-sensitive low-affinity creatine transporter. Also functions as a pH-independent carnitine efflux transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPB7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003749"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004332.1">
        <property type="protein sequence ID" value="ENSGAGP00000003744.1"/>
        <property type="gene ID" value="ENSGAGG00000003043.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000004337.1">
        <property type="protein sequence ID" value="ENSGAGP00000003749.1"/>
        <property type="gene ID" value="ENSGAGG00000003043.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000422">
        <property type="entry name" value="Monocarboxylate transporter 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000127">
        <property type="entry name" value="Monocarboxylate transporter 9 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF158">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="371"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="394"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="459"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="15"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="508" mass="55832" checksum="8E808E177146587E" modified="2019-05-08" version="1">MVLLKSPDGGWGWVIVLVSFFTQFFCYGSPLAVGVLYLEWLDTFGEGKGKTAWIGSLASGVGLLASPVCSACVSSFGARPVTIFSGFMVAGGLMMSSFAPNIYFLFFSYGIIVGLGCGLLYTATVTITCQYFDKRRGLALGLISTGSSVGLFIYAALQRELIELYGLDGCLLIVGALSLNILACGSLMRPLELSNSPMPEKSCLEKVPDQYLIYYEKENVEENTSILEKGYNEEKCVNNMSSHDCKQDSILNKNGLISIHAKETDTYKQKVVEQTFFCKQLAKKKWQLYLNYWEETLFLFKNKVFSSLFIAILLFDIGGFPPLLLMEDVARSSNISEEEYVMPLISIIGIMTAVGKLVLGVLADFKWINTLYLYVITLVMTGVALVAIPFAKSYIMLAILSGILGFLTGNWSIFPYVTTKTVGIEKLAHAYGILMFFAGLGNSLGPPIVGWFYDWTQTYDIAFCFSGLCVLLGGLLLLVAALPCWDNCNNQSAKPPPNTYSYMVASSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQ06</accession>
    <name>A0A452GQ06_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homomultimers may associate with EGR1 bound to DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQ06"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003780"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004373.1">
        <property type="protein sequence ID" value="ENSGAGP00000003780.1"/>
        <property type="gene ID" value="ENSGAGG00000003074.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.2010:FF:000001">
        <property type="entry name" value="NGFI-A-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.2010">
        <property type="entry name" value="NAB conserved domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006989">
        <property type="entry name" value="NAB_co-repressor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039040">
        <property type="entry name" value="NAB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006988">
        <property type="entry name" value="Nab_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038398">
        <property type="entry name" value="NCD2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12623">
        <property type="entry name" value="NGFI-A BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12623:SF6">
        <property type="entry name" value="NGFI-A-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04905">
        <property type="entry name" value="NCD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04904">
        <property type="entry name" value="SAM_NCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Nab N-terminal" evidence="9">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="NAB co-repressor" evidence="10">
        <location>
            <begin position="183"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="108"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="302"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="426"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="454"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="469" mass="50775" checksum="8C63465EA3ADA30C" modified="2019-05-08" version="1">MCLPLAMALPRTLGELQLYRVLQRANLLSYYETFIQQGGDDVQQLCEAGEEEFLEIMALVGMATKPLHVRRLQKALREWAANPGLFSQPMASLPVSSIPLFKISESGGRKSLSNGHASPGEAPGKATPPACSPSEPGEKLSPLPTPQWPGRSTPESEGGGAEEDGGPPPFSPGAGGDPAPEQLDPELARTVAECVERLLQSCPRGGEPGLRTLMKLNKKLAKAVGHIFQLDDGDRRKEAEIRRHSAIYGRGEARRRDGKPLTLHELTINEAAAQFCLRDHTLLLRRVELFSLSRQVARESTYLSSLKGARSHPDESGAVQPKKLKQEVGEQSRPEPLQLPGGSESYAPPYRASQEEDTASLSGESLDGHLQAFSSCPRLTPPPSAAPDLPLSLPPHGLWSRHILQQTLMDEGLRLARLVSHERVGRLSPCLPGKSPGPEFEDGLAEGAPPAQAESHRSSVKVEQETSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQQ6</accession>
    <name>A0A452GQQ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear envelope phosphatase-regulatory subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Transmembrane protein 188</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNEP1R1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQQ6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004265"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005008.1">
        <property type="protein sequence ID" value="ENSGAGP00000004265.1"/>
        <property type="gene ID" value="ENSGAGG00000003542.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071595">
        <property type="term" value="C:Nem1-Spo7 phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019168">
        <property type="entry name" value="NEP1-R1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20996">
        <property type="entry name" value="NUCLEAR ENVELOPE PHOSPHATASE-REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20996:SF1">
        <property type="entry name" value="NUCLEAR ENVELOPE PHOSPHATASE-REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09771">
        <property type="entry name" value="Tmemb_18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="198" mass="22154" checksum="C4E7EF1A6B9990BB" modified="2019-05-08" version="1">MPNGERRCCGGRGIAEGAEGGTRSRLSGLRCERVRGAEEGAAVRSDRPPPPAMNSLEQAEDLKAFERRLTEYISCLQPATGRWRMILIVVSVCTATGAWNWLIDPETQKVSFFTSLWNHPFFTISCITLIGLFFAGIHKRVVAPSIIAARCRTVLAEYNMSCDDVSDSGLTDYMYMFKAEINAKKMKLLFLNRHCIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRG8</accession>
    <name>A0A452GRG8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GRG8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005354.1">
        <property type="protein sequence ID" value="ENSGAGP00000004570.1"/>
        <property type="gene ID" value="ENSGAGG00000003772.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="152" mass="16453" checksum="B1EF7679E5EF7A47" modified="2019-05-08" version="1">MLGREINAEIEQWTRTPPDGLKYGWKGARLPLVPNHQARPLDPVDPGRGGRQLHGLASLHQFTQAALTGPSPCADRRESVPEHSASSRISQSRGEKVCIEGPGPLVHLCRIREGNAVLYQEAGDVLLGPPLFLQETRKNPLSASLPRATSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRQ6</accession>
    <name>A0A452GRQ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GRQ6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004669"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005466.1">
        <property type="protein sequence ID" value="ENSGAGP00000004669.1"/>
        <property type="gene ID" value="ENSGAGG00000003849.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15227">
        <property type="entry name" value="7tmA_OR14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000037">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="34"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="100"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="50"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="303" mass="33657" checksum="B3E6E9FBA8359016" modified="2019-05-08" version="1">MPSTECTVPGVPWNQIESDSRLFLLFIPSKTSSILHFVVFLVIYLAAVMGNLLIITAVAIDHHLHSPMYFFPDNLSFLDACYISVTVPKSMVNSLTNTRLISFSGCVAQVYFGIFAFSELVLLAAMAYDRYVAICHPLRYRVIMTRGACAQMAAGSWLSSCIYSLLHSANTFLLPFCGSNVLDQFFCNIPELLKISCADTSANEIVDILCSAVVGLFFLISILSAVLRIPSAKGTYKAFSTCLPHLVVFSLFISTGIYTYLRLGSQSSQYQGMLATMLYSVRYQNIPILRMVQKHFPRITGTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUK0</accession>
    <name>A0A452GUK0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A452GUK0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005620"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006548.1">
        <property type="protein sequence ID" value="ENSGAGP00000005620.1"/>
        <property type="gene ID" value="ENSGAGG00000004565.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="9">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="119"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="340"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="454" mass="49886" checksum="F0EE3B65BE952D5C" modified="2019-05-08" version="1">MQLRLARGPPRRGRELPVVPTRAGKLLLRSRRPQLSQPRSLTLGRWERPLLVSAGWKHRRSCGDYFQLERSQEQAPALQPPRAAGGDSSFRALGLGPALVSALQSLSITQPTAVQRRAIPALLRGGNALCAAETGSGKTLAYLLPLFQKLLSRPLLGPAWPPCPRSLVLLPSRELAEQVRTVASSLGHSVGLRVQEIGGGRGMASVKNQLRSDPDADLLVSTPGALCKALRKRMVRLERLCCLVLDEADTLLDPTFLDLVEEVLMQAPIAHSPKEVADQWNTKTQLVVVGATFPEGLGQFLSKVTNLGHFTTLTSQSLHHLLPHVQQKFIRIKGSDKASELLQLIKDQGRSNGALLIFCNNASTVNWLGYILDDHKIKHLRLQGQMPAAMRAGIFNTFQKGQRDVLICTDLASRGLDTSRVALVVNYDFPSTLQDYLHRPQNVHLGQSWANYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUK6</accession>
    <name>A0A452GUK6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GUK6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005776"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006735.1">
        <property type="protein sequence ID" value="ENSGAGP00000005776.1"/>
        <property type="gene ID" value="ENSGAGG00000004698.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13954">
        <property type="entry name" value="7tmA_OR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="220"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="64"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="333" mass="37413" checksum="F6DE99D6B0B8B027" modified="2019-05-08" version="1">CSLPLHPVLPRTTVSKLTLSVKMTNMDNQSTVTEFRLLGLSSFPEMQPLLFMCFLIIYLLTLAGNLSICLAIWADTTLHTPMYFFLVNLSLLDISYSSVTLPNMLVTLVAKIKSLSFSSCMAQLYFLISFAGSESFLLALMAYDRYMAICQPLHYATIMNHRACMCYAIAIWIGGFVYSMLHTFFIARLPFCGPNEINHFFCEIPPLIKLACMDTYFNEVLVFLLSGVVGGSCFLLISTSYAYILSTIMKIRSTQGRCKTFSTCASHLLTVLLFYGPGFFTYLHPSSSYSMDTGKVISVFYTVVTPMLNPMIYSLRNKDVQATFKKAVGRSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYM5</accession>
    <name>A0A452GYM5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipocalin/cytosolic fatty-acid binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GYM5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007156"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008281.1">
        <property type="protein sequence ID" value="ENSGAGP00000007156.1"/>
        <property type="gene ID" value="ENSGAGG00000005751.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="2">
        <location>
            <begin position="11"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="132" mass="14889" checksum="BDB61E3E9C121823" modified="2025-10-08" version="2">MPPYHALSRWPDAVSFAGVNVMLRKIAVAAASKPAVEIKQDGETFYIKTSTTVRTTEINFKIGEEFEEQTVDGRPCKSLAKWESENKMVCEQRLLKGEGPKTGWSREMTNDGELILTMTADDVVCTRIYIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1J2</accession>
    <name>A0A452H1J2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H1J2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008490"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009773.1">
        <property type="protein sequence ID" value="ENSGAGP00000008490.1"/>
        <property type="gene ID" value="ENSGAGG00000006749.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15911">
        <property type="entry name" value="7tmA_OR11A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF253">
        <property type="entry name" value="OLFACTORY RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="44"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="320" mass="36081" checksum="AF76E4574EC035B8" modified="2019-05-08" version="1">MEKGEGENQTSISEFILLGFGNLPGLQFLFFLLFLGIYIITMAANLLIVALVAADQHLHNSMYFFLGNLSCLEICYSSTILPRMLASFLTGDRTVSVTGCMVQFYWFGVLLTVECYLLASMSYDRYLAICNPLHYGTLMNVRFCMQLAAGSWLSSFIILATIIYLVSQLAFCGPNEIDHFFCDLTQMINLSCSDTGLVNLVTLIFSSTNIILPFLLTLTSYVYIISTILRISSTTGKQKAFSTCSSHLIVVTIYYGTLITVYMLPNTGALRTLNKFVSMLYTVLTPLINPLIYSLRNKEVKEALRRAQQKYWVFPYILRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2A2</accession>
    <name>A0A452H2A2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein axonemal assembly factor 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Dynein axonemal-associated protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">In cyliated cells, dynein axonemal particle-specific protein required for deployment of ODA to the axoneme. Interacts with outer dynein arm (ODA) subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Dynein axonemal particle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000008633"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009925.1">
        <property type="protein sequence ID" value="ENSGAGP00000008633.1"/>
        <property type="gene ID" value="ENSGAGG00000006842.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031531">
        <property type="entry name" value="DNAAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35977">
        <property type="entry name" value="CHROMOSOME 16 OPEN READING FRAME 71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35977:SF1">
        <property type="entry name" value="DYNEIN AXONEMAL ASSEMBLY FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15773">
        <property type="entry name" value="DAAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="8">
        <location>
            <begin position="1373"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="384"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="493"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="404"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="515"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1712" mass="190697" checksum="3BEBB2B28EC28613" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2Y4</accession>
    <name>A0A452H2Y4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H2Y4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008880"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010206.1">
        <property type="protein sequence ID" value="ENSGAGP00000008880.1"/>
        <property type="gene ID" value="ENSGAGG00000007025.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="92"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="220" mass="24966" checksum="EC84C8D21927DF17" modified="2019-05-08" version="1">MSPMRKSSPGTAAPTKERKRCPWLSFWVILVCALVLKACVIYGCMVTLLMGNPSCEEHKALPQGSAEWHCVMGRDEGKGRVWTCCPLGWEPFQTSCYYFSKDIMNWDNSQRNCTGMGSHLVVINTGAEQDFISNYVKRTVIGIRVENYYIGLSLKKGHWRWVDVTPYNETAVFWIPGEPNNLSVEKCVAVDTSQKDNRNWNNFPCIAPFHRICETAATNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H583</accession>
    <name>A0A452H583_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H583"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009664"/>
    <dbReference type="GlyCosmos" id="A0A452H583">
        <property type="glycosylation" value="1 site, No reported glycans"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000011101.1">
        <property type="protein sequence ID" value="ENSGAGP00000009664.1"/>
        <property type="gene ID" value="ENSGAGG00000007628.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:monoatomic cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032732">
        <property type="term" value="P:positive regulation of interleukin-1 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000990">
        <property type="entry name" value="Innexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039099">
        <property type="entry name" value="Pannexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15759">
        <property type="entry name" value="PANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15759:SF7">
        <property type="entry name" value="PANNEXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51013">
        <property type="entry name" value="PANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="408"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="413"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="12">
        <location>
            <position position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600990-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="616" mass="69973" checksum="4746F67ADF0FA889" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H665</accession>
    <name>A0A452H665_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the chemokine-like receptor (CMKLR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H665"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011661.1">
        <property type="protein sequence ID" value="ENSGAGP00000010163.1"/>
        <property type="gene ID" value="ENSGAGG00000007967.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004875">
        <property type="term" value="F:complement receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000826">
        <property type="entry name" value="Formyl_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225:SF48">
        <property type="entry name" value="C3A ANAPHYLATOXIN CHEMOTACTIC RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225">
        <property type="entry name" value="CHEMOTACTIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="24"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="306" mass="35009" checksum="293F929E3B89B888" modified="2019-05-08" version="1">MDQGATHLAAAMLLFTTLPVGVVGNGLYLWVLGLKMRRMVTKLFFLHLVSCYLLFTLLIPFFAEVLRSRWPFGQALCKLHSFTRYLVVFAGVFVLTLISLHRCLLVAQPVWVRNHHRPRLGCWLITGAWILAICFSIPYLVVRDVENKRGESFCVYRTDLRHFVEMPLRLSRFLGGFLVPFAIIATSYVVLIWKLRRRSWVGSQRTFALVLAVVALFFVCWLPHHVLVLLSTYQNKKEIWGVALKLANALAYLHSCLNPVLYGLVGFTRSKGRRRGSFLGIFRRALAEEEEGSSEMEASQSTRRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H704</accession>
    <name>A0A452H704_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-crystallin A4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Beta-A4 crystallin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homo/heterodimer, or complexes of higher-order. The structure of beta-crystallin oligomers seems to be stabilized through interactions between the N-terminal arms.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H704"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010496"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012049.1">
        <property type="protein sequence ID" value="ENSGAGP00000010496.1"/>
        <property type="gene ID" value="ENSGAGG00000008208.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000004">
        <property type="entry name" value="Crystallin beta A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000002">
        <property type="entry name" value="Crystallin, beta B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF19">
        <property type="entry name" value="BETA-CRYSTALLIN A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="15"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="55"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="199" mass="23100" checksum="175F02669E97A0A0" modified="2019-05-08" version="1">MMPEQPPDPDCRPPPQIVVWDEEFFQGRRQEFTTECYDVRTCGFQTLRSLKIESGAWAGFEHVGFQGQQFVLERGEYPRWEAWSGSHAYHMERMSSFRPIACANHRNSKMTIFEWENFLGRKGDLSDDYPSLPAMGWGSSAVSSFHAHSGAWVCSQFPGYRGFQYILECDCHAGEYKHFREWGSHAQTSQVQSIRRIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7S9</accession>
    <name>A0A452H7S9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Cleavage and polyadenylation specificity factor subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 8">Cleavage and polyadenylation specificity factor 100 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H7S9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010614"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012176.1">
        <property type="protein sequence ID" value="ENSGAGP00000010614.1"/>
        <property type="gene ID" value="ENSGAGG00000008287.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006398">
        <property type="term" value="P:mRNA 3'-end processing by stem-loop binding and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16293">
        <property type="entry name" value="CPSF2-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000008">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027075">
        <property type="entry name" value="CPSF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025069">
        <property type="entry name" value="Cpsf2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035639">
        <property type="entry name" value="CPSF2_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45922">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45922:SF1">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13299">
        <property type="entry name" value="CPSF100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Beta-Casp" evidence="10">
        <location>
            <begin position="243"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="407"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="495"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="407"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="417"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="783" mass="88774" checksum="90492DB271798D9C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H804</accession>
    <name>A0A452H804_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H804"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010692"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012261.1">
        <property type="protein sequence ID" value="ENSGAGP00000010692.1"/>
        <property type="gene ID" value="ENSGAGG00000008344.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050446">
        <property type="entry name" value="FAD-oxidoreductase/Apoptosis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557:SF7">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="4">
        <location>
            <begin position="64"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="246" mass="26824" checksum="3BFC93B55A5A2E41" modified="2019-05-08" version="1">MAHWRLGMAELQTGNRNVLPPCIWVQRADWAGGTRCLCGYALRVLSHAVERSSEPNTACCPSLLQVVSVDMQGQKAHFQDGAFQRYDQLLIATGSSPRQLQCPGSDLENVCSLQTPEDANRILHLATGKRVVILGASFIGMEVAAFLSDKSSAICVVEREEFPFQAVLGPQVGGVAMKMLQNKSVKFYMKAEISELRGENRKVTKSLTPAPHLDCSQNLLPIWAYTIPLPSHVPSTGHVISSLPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H931</accession>
    <name>A0A452H931_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Insulin gene enhancer protein ISL-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H931"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011074"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012681.1">
        <property type="protein sequence ID" value="ENSGAGP00000011074.1"/>
        <property type="gene ID" value="ENSGAGG00000008574.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048665">
        <property type="term" value="P:neuron fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09366">
        <property type="entry name" value="LIM1_Isl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09374">
        <property type="entry name" value="LIM2_Isl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000034">
        <property type="entry name" value="Insulin gene enhancer protein ISL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000041">
        <property type="entry name" value="insulin gene enhancer protein ISL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000056">
        <property type="entry name" value="insulin gene enhancer protein ISL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047169">
        <property type="entry name" value="ISL1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047244">
        <property type="entry name" value="ISL1/2-like_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24204">
        <property type="entry name" value="INSULIN GENE ENHANCER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24204:SF4">
        <property type="entry name" value="INSULIN GENE ENHANCER PROTEIN ISL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9 15 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 15 17" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 16" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="19">
        <location>
            <begin position="15"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="19">
        <location>
            <begin position="78"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="20">
        <location>
            <begin position="179"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="15">
        <location>
            <begin position="181"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="312"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="349" mass="39036" checksum="DBE095D70E84BEE4" modified="2019-05-08" version="1">MGDMGDPPKKKRLISLCVGCGNQIHDQYILRVSPDLEWHAACLKCAECNQYLDETCTCFVRDGKTYCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALRDDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEPISARQPALRPHVHKQPEKTTRVRTVLNEKQLHTLRTCYAANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQANPVEVQSYQPPWKVLSDFALQSDIDQPAFQQLVNFSEGGPGSNSTGSEVASMSSQLPDTPNSMVASPIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HA33</accession>
    <name>A0A452HA33_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Delta-1-pyrroline-5-carboxylate synthase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="20">Glutamate 5-kinase</fullName>
                <shortName evidence="20">GK</shortName>
                <ecNumber evidence="20">2.7.2.11</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="20">Gamma-glutamyl kinase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="20">Gamma-glutamyl phosphate reductase</fullName>
                <shortName evidence="20">GPR</shortName>
                <ecNumber evidence="20">1.2.1.41</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="20">Glutamate-5-semialdehyde dehydrogenase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="20">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 20">
            <text>L-glutamate + ATP = L-glutamyl 5-phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14877"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 20">
            <text>L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 20">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 20">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Can form homodimers/multimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">In the N-terminal section; belongs to the glutamate 5-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.11" evidence="20"/>
    <dbReference type="EC" id="1.2.1.41" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A452HA33"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011574"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013250.1">
        <property type="protein sequence ID" value="ENSGAGP00000011574.1"/>
        <property type="gene ID" value="ENSGAGG00000008932.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00359"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004349">
        <property type="term" value="F:glutamate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04256">
        <property type="entry name" value="AAK_P5CS_ProBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000010">
        <property type="entry name" value="Delta-1-pyrroline-5-carboxylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000011">
        <property type="entry name" value="Delta-1-pyrroline-5-carboxylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00456">
        <property type="entry name" value="ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041744">
        <property type="entry name" value="G5K_ProBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005715">
        <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019797">
        <property type="entry name" value="Glutamate_5-kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005766">
        <property type="entry name" value="P5_carboxy_syn"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01092">
        <property type="entry name" value="P5CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001221">
        <property type="entry name" value="PRK00197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01027">
        <property type="entry name" value="proB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063:SF8">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063">
        <property type="entry name" value="GLUTAMATE SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036429">
        <property type="entry name" value="P5C_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00902">
        <property type="entry name" value="GLUTAMATE_5_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 20" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12 20" id="KW-0521">NADP</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 20" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="22">
        <location>
            <begin position="76"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="21">
        <location>
            <begin position="368"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR036429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="758" mass="82999" checksum="D83B1F52C1DADFFA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAT0</accession>
    <name>A0A452HAT0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SKA complex subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Spindle and kinetochore-associated protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SKA1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HAT0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011840"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013556.1">
        <property type="protein sequence ID" value="ENSGAGP00000011840.1"/>
        <property type="gene ID" value="ENSGAGG00000009123.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000940">
        <property type="term" value="C:outer kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031110">
        <property type="term" value="P:regulation of microtubule polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12958">
        <property type="entry name" value="SKA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1890">
        <property type="entry name" value="Ska1 microtubule binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009829">
        <property type="entry name" value="SKA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042031">
        <property type="entry name" value="SKA1_MBD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28573">
        <property type="entry name" value="SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28573:SF1">
        <property type="entry name" value="SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07160">
        <property type="entry name" value="SKA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00047182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="208" mass="23838" checksum="6F294E5961EFB545" modified="2019-05-08" version="1">MASSDLEDLCSHINTKILTIKKTLQLRNMGQEPSLKSVLCKIGHEMFLLNDLLNKMEIEVEHQEKLKNSLKEMQESFERDYNEAQHLCENIPPHLPKAIPKCTVGLIVKSEEQCKAAEPECVKKPTKEPKFIKEAPLITAQEFEGVPAYMRGRLTYVQINGVVQDLNKAVVSKYKILHQPMKSMSSAVRNLYHRFLEEETKDTKGILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAZ4</accession>
    <name>A0A452HAZ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452HAZ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011901"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013632.1">
        <property type="protein sequence ID" value="ENSGAGP00000011901.1"/>
        <property type="gene ID" value="ENSGAGG00000009178.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000013636.1">
        <property type="protein sequence ID" value="ENSGAGP00000011905.1"/>
        <property type="gene ID" value="ENSGAGG00000009178.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000761">
        <property type="entry name" value="SRSF protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000105">
        <property type="entry name" value="SRSF protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000163">
        <property type="entry name" value="SRSF protein kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF4">
        <property type="entry name" value="SRSF PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="114"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="40"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="272"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="421"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="495"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="332"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="421"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="442"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="503"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="695" mass="78825" checksum="A6869BFFCB82B3DD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCI5</accession>
    <name>A0A452HCI5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000012496"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014322.1">
        <property type="protein sequence ID" value="ENSGAGP00000012496.1"/>
        <property type="gene ID" value="ENSGAGG00000009601.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06745">
        <property type="entry name" value="PDZ_SIPA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050989">
        <property type="entry name" value="Rap1_Ran_GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021818">
        <property type="entry name" value="SIPA1L_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711">
        <property type="entry name" value="RAP GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711:SF7">
        <property type="entry name" value="SIGNAL-INDUCED PROLIFERATION-ASSOCIATED 1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21022">
        <property type="entry name" value="Rap-GAP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11881">
        <property type="entry name" value="SPAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="6">
        <location>
            <begin position="588"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="969"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="353"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1120"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1305"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1344"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1676"/>
            <end position="1724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="353"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1138"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1183"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1213"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1238"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1314"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1356"/>
            <end position="1376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1740" mass="193279" checksum="B9BCC17614668FC3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDB0</accession>
    <name>A0A452HDB0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">vesicle-fusing ATPase</fullName>
            <ecNumber evidence="3">3.6.4.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452HDB0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012781"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014648.1">
        <property type="protein sequence ID" value="ENSGAGP00000012781.1"/>
        <property type="gene ID" value="ENSGAGG00000009807.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02678">
        <property type="entry name" value="MIT_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19521">
        <property type="entry name" value="RecA-like_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000043">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000015">
        <property type="entry name" value="vacuolar protein sorting-associated protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045253">
        <property type="entry name" value="VPS4_MIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF83">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MIT" evidence="16">
        <location>
            <begin position="2"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="159"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="437" mass="48979" checksum="CB2E3232E16CB3C5" modified="2019-05-08" version="1">MSTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCMQYLDRAEKLKDYLRNKDKQSKKPVKESPNDSKGSDSDSEGENPEKKKLQEQLMGAIVMEKPNVRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAGRRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGNTPRLLTEANIHELARKTDGYSGADISVIVRDALMQPVRKVQSATHFKKVRGPSRTNPSITVDDLLTPCSPGDPGAIEMTWMEVPSDKLLEPVICMSDMLRSLATTRPTVNAEDLLKVKKFTEDFGQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDE6</accession>
    <name>A0A452HDE6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutathione transferase</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Theta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452HDE6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012813"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014687.1">
        <property type="protein sequence ID" value="ENSGAGP00000012813.1"/>
        <property type="gene ID" value="ENSGAGG00000009836.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03183">
        <property type="entry name" value="GST_C_Theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03050">
        <property type="entry name" value="GST_N_Theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000008">
        <property type="entry name" value="Glutathione S-transferase theta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000086">
        <property type="entry name" value="Glutathione S-transferase theta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040077">
        <property type="entry name" value="GST_C_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040075">
        <property type="entry name" value="GST_N_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051369">
        <property type="entry name" value="GST_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917:SF9">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE THETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="9">
        <location>
            <begin position="88"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="239" mass="27543" checksum="1369FDC7777E1213" modified="2019-05-08" version="1">MVVELYLDLLSQPCRALFIFAKKNNIPFEFKHVELLKGQHLSEEFGKVNVLKKVPALKDGAFTLAESIAILLYLSRKFNTPDHWYPSDLQKRARVDEYLSWQHTTIREKGCQIFIIKVLVPLLIGQPFSPEKLEEAVKELNSVLNQFEEKFLQNKPFIVGNEISLADLMAIVELMEPVGCGCDIFENRPKLVEWRSRVEEAVGKELFQQAHEILLNAKNLSTTQLPPELRESMKRLLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDG2</accession>
    <name>A0A452HDG2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calcineurin-binding protein cabin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Calcineurin inhibitor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000012839"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014714.1">
        <property type="protein sequence ID" value="ENSGAGP00000012839.1"/>
        <property type="gene ID" value="ENSGAGG00000009851.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13839">
        <property type="entry name" value="MEF2_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000654">
        <property type="entry name" value="Calcineurin-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000076">
        <property type="entry name" value="calcineurin-binding protein cabin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033053">
        <property type="entry name" value="Hir3/CABIN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015134">
        <property type="entry name" value="MEF2-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502:SF7">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09047">
        <property type="entry name" value="MEF2_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="90"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="124"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-binding protein cabin-1 MEF2-binding" evidence="11">
        <location>
            <begin position="2121"/>
            <end position="2155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="364"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1295"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1424"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1746"/>
            <end position="1836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1954"/>
            <end position="1984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2056"/>
            <end position="2088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2163"/>
            <end position="2185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1298"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1329"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1374"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1398"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1759"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1784"/>
            <end position="1794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1796"/>
            <end position="1813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1959"/>
            <end position="1974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="2056"/>
            <end position="2065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="2071"/>
            <end position="2082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="2172"/>
            <end position="2185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2185" mass="246067" checksum="C700D955455B05AD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HE42</accession>
    <name>A0A452HE42_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HE42"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013073"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014974.1">
        <property type="protein sequence ID" value="ENSGAGP00000013073.1"/>
        <property type="gene ID" value="ENSGAGG00000010031.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000028">
        <property type="entry name" value="Protocadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000034">
        <property type="entry name" value="Protocadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000043">
        <property type="entry name" value="Protocadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000082">
        <property type="entry name" value="Protocadherin 7b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000005">
        <property type="entry name" value="Protocadherin 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000016">
        <property type="entry name" value="Protocadherin 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000073">
        <property type="entry name" value="protocadherin-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013585">
        <property type="entry name" value="Protocadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF253">
        <property type="entry name" value="PROTOCADHERIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08374">
        <property type="entry name" value="Protocadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="875"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="55"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="147"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="294"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="415"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="522"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="632"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="742"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="186"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="906"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="907"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="927"/>
            <end position="941"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1088" mass="118362" checksum="7F86AF0DAD1F290C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGB7</accession>
    <name>A0A452HGB7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">PR domain zinc finger protein 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">PR domain-containing protein 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGB7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013915"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015934.1">
        <property type="protein sequence ID" value="ENSGAGP00000013915.1"/>
        <property type="gene ID" value="ENSGAGG00000010605.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014003">
        <property type="term" value="P:oligodendrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000012">
        <property type="entry name" value="PR domain zinc finger protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052296">
        <property type="entry name" value="TR-Histone_Methyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16516">
        <property type="entry name" value="AGAP007109-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16516:SF5">
        <property type="entry name" value="ZINC FINGER PROTEIN 488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="19">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="471"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="513"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="427"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="536" mass="60550" checksum="FF955E8706311FEF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHI3</accession>
    <name>A0A452HHI3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HHI3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014347"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016415.1">
        <property type="protein sequence ID" value="ENSGAGP00000014347.1"/>
        <property type="gene ID" value="ENSGAGG00000010907.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="78" mass="8323" checksum="E77494ED809AEC04" modified="2019-05-08" version="1">IFCVLSLTSALTIHILGNFNVVGNLVLSHLLFGSHLSGLSYAGISLTLSGMFLYHNCDLIASYWGSRLALGQGQAKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHL2</accession>
    <name>A0A452HHL2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HHL2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014378"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016449.1">
        <property type="protein sequence ID" value="ENSGAGP00000014378.1"/>
        <property type="gene ID" value="ENSGAGG00000010927.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000034">
        <property type="entry name" value="SH2 domain-containing protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14388:SF5">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14388">
        <property type="entry name" value="T CELL-SPECIFIC ADAPTER PROTEIN TSAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="4">
        <location>
            <begin position="342"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="120"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="269"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="448" mass="50664" checksum="43EFEEB619B100EE" modified="2019-05-08" version="1">MLQQILADMYIDPELLAELSEEQKQILFLKMRQEQVRRWKETEAAADKNSAKKVTQRKAHGKSVKWKLGADSEVWVWVMGEHTSDKPYDVICEEIQAWRAQQEAGEARGSQTFLEPAAPWSLHSQPGALGGADAKRKEEDMITAAKKERSWDRAQAAPSKGRDVQQMLADSKAQTRKYDFQQGKDLARRRPGGMRASAERALHTNTDRKEAQESGQKSDKDDPEWQESLRKSKAADERRRSIAKQARDDYNRLSLQGIRKGKVAEVSKNFGSKERSLPPRPSLPPKPKTLNSGVANGRPHRNPGVQRTISTSTQESIVRWFKEEQFPLRAGYEKSTDRIAPWFHGIVTLRQAEELLSKSVPGSFLVRVSEKIKGYALSLLSPEGCKHFLIDASSNSYSFLGVDQLQHSTLADLVNYHKEEPITSLGKELLLYPCGQQGPEPDYLELFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHL7</accession>
    <name>A0A452HHL7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Transcobalamin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Transcobalamin II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with CD320 (via LDL-receptor class A domains).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic cobalamin transport proteins family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HHL7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014341"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016408.1">
        <property type="protein sequence ID" value="ENSGAGP00000014341.1"/>
        <property type="gene ID" value="ENSGAGG00000010902.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015889">
        <property type="term" value="P:cobalamin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002157">
        <property type="entry name" value="Cbl-bd_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051588">
        <property type="entry name" value="Cobalamin_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027954">
        <property type="entry name" value="Transcobalamin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10559">
        <property type="entry name" value="TRANSCOBALAMIN-1/GASTRIC INTRINSIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10559:SF14">
        <property type="entry name" value="TRANSCOBALAMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01122">
        <property type="entry name" value="Cobalamin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14478">
        <property type="entry name" value="DUF4430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3" id="KW-0171">Cobalt transport</keyword>
    <keyword evidence="9 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019350866" description="Transcobalamin-2" evidence="17">
        <location>
            <begin position="22"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Transcobalamin-like C-terminal" evidence="18">
        <location>
            <begin position="357"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="148"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>cyanocob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:17439"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>cyanocob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:17439"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>cyanocob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:17439"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>cyanocob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:17439"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="404"/>
            <end position="406"/>
        </location>
        <ligand>
            <name>cyanocob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:17439"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>cyanocob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:17439"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>cyanocob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:17439"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="22"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="161"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR602157-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602157-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="436" mass="48510" checksum="66DCF0FAAB906CF8" modified="2019-05-08" version="1">MEPRMLQLLFVLHVLALPVQLCEIPRGNPALIKCLNMELLRLSQDLSEELNPSVYVALRLSADHSLEKEAQYLQRLKNVFQPSASSSKAGGQQKQPNTGRLALYLLALRAACQDMETLLERRLVTRLKHHLHKEKEQIAFKRNGRPITSYYQYSLGVLALCVHGKKIDSHVIQKLLNAEQHNKFTHGSKLSVDTTAMAGLAFACLKQANLYEPKLVAAISLAVQRVTNRILQAQTTRGTFGNIFSSPLAMQLLIATRMSKKPVCPKGMDALLGSLEQGHFQNPMIMSQLLPVLDGKSYLDIATMECQAEQDTLELDTQCPGSRTVILGTKMITVQLAVEHPPNPETLYNHSIQVLSGSSLQDMLRTAAEQGPGHFTFETQNTLHGPFLTAVMGVKASNGERRYWQLLQAPDTSLQKGISDYHPQDGESIILKFSPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHR1</accession>
    <name>A0A452HHR1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HHR1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014390"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016461.1">
        <property type="protein sequence ID" value="ENSGAGP00000014390.1"/>
        <property type="gene ID" value="ENSGAGG00000010938.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15222">
        <property type="entry name" value="7tmA_OR51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000002">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF29">
        <property type="entry name" value="OLFACTORY RECEPTOR FAMILY 51 SUBFAMILY AF MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="48"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="336" mass="37511" checksum="0CE90567CBE36F3F" modified="2019-05-08" version="1">QTEECLSNSCPSSGQTPMLLLTGIPGLEDIHLWISIPFCLMYVISIAGNSVILFVIKTDPSLHEPMYIFLSMLAITDLGLSVTTMPTILGIFLFKSREISLDACFAQLFFIHSLSYIESSVLLLMAFDRFVAICNPLRYTSILTLPKIAKMGLVCVLRAVALIFPLLFLLKRFQYCRANVLSHSYCVHQEVMKLACSDITVNSIYGLSIALLTVGLDSLLICLSYIMILRAVRSITSNLESLRALNTCVAHLSAVLLFYTPMLGLPVIHRFGNSSSHSLQIFLGYVYLLVPPLMNPIVYSVKSKHLRVRIIKVFFKDKKHGPQPPIPSWTVTSEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HII4</accession>
    <name>A0A452HII4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDNF/GAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GDNFR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HII4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014679"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016786.1">
        <property type="protein sequence ID" value="ENSGAGP00000014679.1"/>
        <property type="gene ID" value="ENSGAGG00000011144.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.110">
        <property type="entry name" value="GDNF binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016017">
        <property type="entry name" value="GDNF/GAS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037193">
        <property type="entry name" value="GDNF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003438">
        <property type="entry name" value="GDNF_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269:SF3">
        <property type="entry name" value="GDNF FAMILY RECEPTOR ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269">
        <property type="entry name" value="GDNF RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02351">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01316">
        <property type="entry name" value="GDNFRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00907">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110035">
        <property type="entry name" value="GDNF receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GDNF/GAS1" evidence="8">
        <location>
            <begin position="26"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="8">
        <location>
            <begin position="115"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="289" mass="32000" checksum="2E297CF53C2DF29C" modified="2019-05-08" version="1">MELTRCVGWAYRANKLEPALSKGNNCLDAAKACNLNDTCKRFRSAYITPCTSSTSNEICNKRKCHKALRLFFDKVPPKHSYGMLFCSCRDIACTERRRQTIVPVCSYEDKEKPNCLNLQDSSVRVKPAPQPPVPALSPPQTQSPLLHPRSLIPWLTPEPGSPNPMCCSLLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDIEECLKFLNFFQDNTCLKNAIQAFGNGTDVTVWQPILPVQTTTATTTTASRFKSMGSESTNNEISTHNDLPACANLQVRLLHKCYTKIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIT9</accession>
    <name>A0A452HIT9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HIT9"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016935.1">
        <property type="protein sequence ID" value="ENSGAGP00000014822.1"/>
        <property type="gene ID" value="ENSGAGG00000011224.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15787" checksum="9C6ADF305FFC6E5B" modified="2019-05-08" version="1">KRCFLRVTERFYTVDQYHQCIKAKTEHWGNDLHRIMALSWTIAAENLAFLRYRCFRANPTQQLEYICCLNNMASVFPNTFIALRILLMLHITVASAEHIFSKPKPKLKCVIFHVSNVVSGLETICQKCFTRSTGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKN5</accession>
    <name>A0A452HKN5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GRB7/10/14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HKN5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015477"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017679.1">
        <property type="protein sequence ID" value="ENSGAGP00000015477.1"/>
        <property type="gene ID" value="ENSGAGG00000011686.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005158">
        <property type="term" value="F:insulin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046627">
        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01259">
        <property type="entry name" value="PH_APBB1IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16141">
        <property type="entry name" value="RA_GRB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10415">
        <property type="entry name" value="SH2_Grb10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000062">
        <property type="entry name" value="growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000056">
        <property type="entry name" value="growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000015">
        <property type="entry name" value="Growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015042">
        <property type="entry name" value="BPS-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039664">
        <property type="entry name" value="GRB/APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035037">
        <property type="entry name" value="Grb10_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039665">
        <property type="entry name" value="PH_APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243:SF4">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08947">
        <property type="entry name" value="BPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="Ras-associating" evidence="9">
        <location>
            <begin position="166"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="292"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="7">
        <location>
            <begin position="495"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="596" mass="67953" checksum="2147B94C0D7A6E32" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HME2</accession>
    <name>A0A452HME2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cysteine-rich protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Interacts with TGFB1I1.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HME2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016114"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018401.1">
        <property type="protein sequence ID" value="ENSGAGP00000016114.1"/>
        <property type="gene ID" value="ENSGAGG00000012083.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09401">
        <property type="entry name" value="LIM_TLP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000025">
        <property type="entry name" value="Cysteine-rich protein 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46074:SF2">
        <property type="entry name" value="CYSTEINE-RICH PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46074">
        <property type="entry name" value="CYSTEINE-RICH PROTEIN CRIP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 9" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="11">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="11">
        <location>
            <begin position="138"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="79"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="222" mass="24430" checksum="07AB3BE338F1093F" modified="2019-05-08" version="1">MQTKCRTHLFSEKVTSLGKDWHKFCLKCERCNKTLTPGGHAEHDGKPFCHKPCYATLFGPKGVNIGGAGSYIYEKPQMEEQPAPGPIEHISKVEEKKVSGPPKGPSRGECRRGQEAGKPKLFSCLSASSVTTFTGEPNMCPRCGKKVYFAEKVTSLGKDWHRPCLRCERCGKTLTPGGHAEHDEQPYCHKPCYGILFGPKGVNTGAVGSYIYDKDPEAKDQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMH5</accession>
    <name>A0A452HMH5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fructose-2,6-bisphosphatase TIGAR</fullName>
            <ecNumber evidence="2">3.1.3.46</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">TP53-induced glycolysis and apoptosis regulator</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 2,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <dbReference type="EC" id="3.1.3.46"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.46" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452HMH5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016195"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018490.1">
        <property type="protein sequence ID" value="ENSGAGP00000016195.1"/>
        <property type="gene ID" value="ENSGAGG00000012153.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045820">
        <property type="term" value="P:negative regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043456">
        <property type="term" value="P:regulation of pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051695">
        <property type="entry name" value="Phosphoglycerate_Mutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517:SF1">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="7">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="275" mass="30394" checksum="41041CC36470DAB6" modified="2019-05-08" version="1">MVRFALTIVRHGETRYNKDKILQGQGVDEPLSATGFRQADAAGIFLSNVKFTHVFSSDLLRAKQTASTIIGKNKFCKDIVIQYDARLRERKYGIAEGRPLSDLKAMANAAGEQCPSFTPSGGETLDEVRTRAKNFFEFLCQLSIEEECQKEQAVLGMAGNGLETLEGKPVFPLRNHCCGLELNSDTDKDTKMLNPNILVVSHGAYMRNWFGYFVLDLKCTLPSALKKSQLSSVSPNTGVSHFIINLGNGDVIKPEISCICLNRDDHLADVNTENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HN40</accession>
    <name>A0A452HN40_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Collagen alpha-1(XII) chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Trimer of identical chains each containing 190 kDa of non-triple-helical sequences.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000016369"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018679.1">
        <property type="protein sequence ID" value="ENSGAGP00000016369.1"/>
        <property type="gene ID" value="ENSGAGG00000011657.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005614">
        <property type="term" value="C:interstitial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035987">
        <property type="term" value="P:endodermal cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="CDD" id="cd01482">
        <property type="entry name" value="vWA_collagen_alphaI-XII-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000018">
        <property type="entry name" value="collagen alpha-1(XII) chain isoform X1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000489">
        <property type="entry name" value="collagen alpha-1(XII) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000554">
        <property type="entry name" value="collagen alpha-1(XII) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000639">
        <property type="entry name" value="collagen alpha-1(XII) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000704">
        <property type="entry name" value="collagen alpha-1(XII) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000816">
        <property type="entry name" value="collagen alpha-1(XII) chain isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000008">
        <property type="entry name" value="Collagen type XII alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000121">
        <property type="entry name" value="Collagen type XII alpha 1 chain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000234">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000480">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000001">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF17">
        <property type="entry name" value="COLLAGEN ALPHA-1(XII) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0176">Collagen</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="7" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="14"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="123"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="319"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="423"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="617"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="708"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="803"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="890"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="981"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1071"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="1180"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1368"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1458"/>
            <end position="1549"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1550"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1637"/>
            <end position="1727"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1728"/>
            <end position="1822"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1909"/>
            <end position="1999"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="2000"/>
            <end position="2090"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="2091"/>
            <end position="2180"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="2181"/>
            <end position="2269"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="2297"/>
            <end position="2470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1054"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1711"/>
            <end position="1730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1984"/>
            <end position="2003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="2734"/>
            <end position="2805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="2841"/>
            <end position="3016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1986"/>
            <end position="1998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="2734"/>
            <end position="2749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="2763"/>
            <end position="2773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="2774"/>
            <end position="2805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="2850"/>
            <end position="2859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="2866"/>
            <end position="2875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="2916"/>
            <end position="2929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="2954"/>
            <end position="2963"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="3016" mass="330310" checksum="F739465BD0235B25" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNB2</accession>
    <name>A0A452HNB2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transmembrane protein 201 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HNB2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016437"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018760.1">
        <property type="protein sequence ID" value="ENSGAGP00000016437.1"/>
        <property type="gene ID" value="ENSGAGG00000012300.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005521">
        <property type="term" value="F:lamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040041">
        <property type="entry name" value="TMEM201"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018861">
        <property type="entry name" value="TMEM201_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28646">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28646:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10476">
        <property type="entry name" value="DUF2448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transmembrane protein 201 C-terminal" evidence="1">
        <location>
            <begin position="105"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="446" mass="48575" checksum="5965E49AF0E27F9E" modified="2025-10-08" version="2">MILGSAQGWKCSNWQHWPPVPLQCSQAIPQNGQISSLYCSLMNGAVVYQSRWGALQLIIDWEVAYNLLQSVHPRLPPTSPPQCLPPGPWLVCRHVALTRSLVFWHSRLRRIDAFASVLWLLVMGLHLAEKYLKADMPSWLDTAKFGTTSLCCLVGFTAAVATRKSTGQRRYRPRRFLSGDSVALFPNGSGIGFPCSAASPSVFIPTPPSILQLTNQQFFRSPRRASSSSLPGRLNRALSLGTMPSLARTDSGYLFSGSRPASQGSQSKESPASEYYSLLSGSCAPSPIPSPAPSVTGSITSSSGSLRYRRPLISPARLNLKGQKLLLFPSQSDALHTSISLDEPTHPDSNVFAAELTPFPRKNLNERGMHDKRSVVERGSICSNNSIKKEDNSSHSSTCVVDTTTKGEDLAGWRGHVGNSVIRGLLAMSLTANAIFTSAYLYQSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNY5</accession>
    <name>A0A452HNY5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin interacting protein 3-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000016681"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019047.1">
        <property type="protein sequence ID" value="ENSGAGP00000016681.1"/>
        <property type="gene ID" value="ENSGAGG00000012471.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1540:FF:000001">
        <property type="entry name" value="SRC kinase signaling inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1540">
        <property type="entry name" value="Actin interacting protein 3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022782">
        <property type="entry name" value="AIP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051825">
        <property type="entry name" value="SRCIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741">
        <property type="entry name" value="P140CAP/SNIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741:SF11">
        <property type="entry name" value="SICKLE TAIL PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03915">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Actin interacting protein 3-like C-terminal" evidence="8">
        <location>
            <begin position="92"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="20"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="486"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="947"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1001"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1058"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1518"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1590"/>
            <end position="1853"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="551"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1065"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1098"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1118"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1525"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1545"/>
            <end position="1556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1639"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1675"/>
            <end position="1687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1715"/>
            <end position="1737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1741"/>
            <end position="1781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1803"/>
            <end position="1818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1826"/>
            <end position="1853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1853" mass="204531" checksum="41B1C0FCEACF7048" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSC5</accession>
    <name>A0A452HSC5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Protein N-terminal glutamine amidohydrolase</fullName>
            <ecNumber evidence="4 8">3.5.1.122</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein NH2-terminal glutamine deamidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>N-terminal L-glutaminyl-[protein] + H2O = N-terminal L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:50680"/>
            <dbReference type="Rhea" id="RHEA-COMP:12668"/>
            <dbReference type="Rhea" id="RHEA-COMP:12777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:64721"/>
            <dbReference type="ChEBI" id="CHEBI:64722"/>
            <dbReference type="EC" id="3.5.1.122"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NTAQ1 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.122" evidence="4 8"/>
    <dbReference type="AlphaFoldDB" id="A0A452HSC5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017968"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020494.1">
        <property type="protein sequence ID" value="ENSGAGP00000017968.1"/>
        <property type="gene ID" value="ENSGAGG00000013320.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008418">
        <property type="term" value="F:protein-N-terminal asparagine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070773">
        <property type="term" value="F:protein-N-terminal glutamine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.620.10:FF:000001">
        <property type="entry name" value="Blast:Protein N-terminal glutamine amidohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.10">
        <property type="entry name" value="Protein N-terminal glutamine amidohydrolase, alpha beta roll"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037132">
        <property type="entry name" value="N_Gln_amidohydro_ab_roll_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039733">
        <property type="entry name" value="NTAQ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023128">
        <property type="entry name" value="Prot_N_Gln_amidohydro_ab_roll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13035">
        <property type="entry name" value="PROTEIN N-TERMINAL GLUTAMINE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13035:SF0">
        <property type="entry name" value="PROTEIN N-TERMINAL GLUTAMINE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09764">
        <property type="entry name" value="Nt_Gln_amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein N-terminal glutamine amidohydrolase alpha beta roll" evidence="9">
        <location>
            <begin position="25"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="206" mass="23735" checksum="85BB5599E28F0CA3" modified="2019-05-08" version="1">MEQPGVAAAVAGYQSVVPARHACAYTSCYCEENVWKLCEYIRSYDQYPLEEFYAVFISNERRMIPLWKQQSGCGDEPVVWDYHVILLHVASGDQNFIYDLDSVLPFPCPFDTYIEESFKSDDILLPGFRRKIRLIQADLYLKTFASDRSHMKDASGHWQKPPPSYPCIETADSKMNLDDFISMNPQVGWGSVFLLPDFVDRYGRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTX2</accession>
    <name>A0A452HTX2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">adenylate cyclase</fullName>
            <ecNumber evidence="5">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452HTX2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018492"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021080.1">
        <property type="protein sequence ID" value="ENSGAGP00000018492.1"/>
        <property type="gene ID" value="ENSGAGG00000013693.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000001">
        <property type="entry name" value="Adenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF7">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="18">
        <location>
            <begin position="135"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="287" mass="32516" checksum="E9A588EB03B8FA66" modified="2019-05-08" version="1">MLIIEFIYVLIVEVPEVNLFDNADLLVAANSFEFSNATSTCSPKASRVALKIVTPIVITVFVLALYLHAQQVESTARLDFLWKLQATEEKEEMEELQAYNRRLLHNILPKDVAAHFLAQERRNDELYYQSCECVAVMFASISNFSEFYVELEANNEGVECLRLLNEIIADFDEIISEDQFRQLEKIKTIGSTYMAASGLNDSTYDKVGKSHIKALADFAMRLMDQMKYINEHSFNNFQMKIGLNIGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGVPDRIQVSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWQ8</accession>
    <name>A0A452HWQ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HWQ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019576"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022289.1">
        <property type="protein sequence ID" value="ENSGAGP00000019576.1"/>
        <property type="gene ID" value="ENSGAGG00000014433.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05726">
        <property type="entry name" value="IgI_4_Robo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20952">
        <property type="entry name" value="IgI_5_Robo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000053">
        <property type="entry name" value="Roundabout guidance receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000055">
        <property type="entry name" value="roundabout homolog 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000065">
        <property type="entry name" value="roundabout homolog 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000008">
        <property type="entry name" value="roundabout homolog 2 isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000058">
        <property type="entry name" value="roundabout homolog 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF242">
        <property type="entry name" value="ROUNDABOUT HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="677"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="37"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="130"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="233"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="341"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="454"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="556"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="849"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="937"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1049"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1121"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="875"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="958"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1059"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1090"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1137"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1148"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1197"/>
            <end position="1221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1279" mass="139369" checksum="284B89E6C29091DA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZC0</accession>
    <name>A0A452HZC0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZC0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020585"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023445.1">
        <property type="protein sequence ID" value="ENSGAGP00000020585.1"/>
        <property type="gene ID" value="ENSGAGG00000015155.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15912">
        <property type="entry name" value="7tmA_OR6C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047132">
        <property type="entry name" value="Olfact_rcpt_6C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454:SF31">
        <property type="entry name" value="OLFACTORY RECEPTOR 214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="309" mass="34756" checksum="B35B35644253CD16" modified="2019-05-08" version="1">MVLANQSTVKEFVLLGFPHLHHLGFLLFLVHLMIYLLTLAGNVVIIAITWADHRLHTPMYFFLRSFSFLEICFTSVIVPKMLANFVSEDKTISFAGCIAQAFFYLSLGVSEFLLLAAMSVDRYVAICYPLRYMAIMNGRVCTFMVLTSWFGGFFFIIGLIMLISRLSFCGPNVLNHFFCDLGPLVSLSCSNTHLVESMDFLVAAVVLLGSLAITVLSYSYIVATVIRIPSAKERRKAFSTCSSHIIVLSIFYGSCIFMYVRPKQRDGLDITKGVAIVNTVITPMLNPFIYSLRNKQVIQVHLAQYPVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1E4</accession>
    <name>A0A452I1E4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I1E4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024246.1">
        <property type="protein sequence ID" value="ENSGAGP00000021290.1"/>
        <property type="gene ID" value="ENSGAGG00000015630.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="158" mass="18491" checksum="62AAE68E8007317B" modified="2019-05-08" version="1">SKSRKTFLSQKAILLFILNTTLFKITVSIFFCTFIMKTGCLQLCKLACIFPRGSSSCSNIRYIKGCPNMLMVCLRALVDNMCDLYPYIFGQIIYRFVEWFFCFFVFFLVLSSLWARHLDASDIQKLKHIIVLFSLRNTPVPQNVFLEHKMETIPQTHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1M9</accession>
    <name>A0A452I1M9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deoxynucleoside kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2'-deoxycytidine + a ribonucleoside 5'-triphosphate = dCMP + a ribonucleoside 5'-diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15698"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="EC" id="2.7.1.74"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2'-deoxyguanosine + ATP = dGMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19201"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57673"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.113"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCK/DGK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I1M9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021374"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024346.1">
        <property type="protein sequence ID" value="ENSGAGP00000021374.1"/>
        <property type="gene ID" value="ENSGAGG00000015694.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004136">
        <property type="term" value="F:deoxyadenosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004137">
        <property type="term" value="F:deoxycytidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004138">
        <property type="term" value="F:deoxyguanosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01673">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000461">
        <property type="entry name" value="Deoxycytidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050566">
        <property type="entry name" value="Deoxyribonucleoside_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513:SF8">
        <property type="entry name" value="DEOXYGUANOSINE KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513">
        <property type="entry name" value="DEOXYNUCLEOSIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Deoxynucleoside kinase" evidence="9">
        <location>
            <begin position="33"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="320" mass="36985" checksum="E672CD13FAC839E8" modified="2019-05-08" version="1">MRPLLSLRLRRAAAGALPRCFGKARRGGSAWRLSIEGNIAVGKSTFLRLLGKTFPEWQMATEPVAKWQKVQASSTRETSSSQNFGNLLQMVYQEPSRWSYTFQTYSCMSRLKAQLEPLSEKLLKTPDPVQIFERSVYSDRYIFAKNLFELGHLTEIEWTIYQDLHTFLLQEFGDRTALHGFLYLQATPEKCLERLHRRGRPEEKAIQLEYLEQLHTQHENWLVKKTTEVHFENLRNAPVFVLNVNHDFEDDPAEQEELMRKPSVMYCPISLDCSCSNITLFSDPISPHKLISQARSAIGQEISRCFVCMLATVSSILAWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I253</accession>
    <name>A0A452I253_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Postsynaptic cell membrane</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the LRRTM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I253"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021565"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024558.1">
        <property type="protein sequence ID" value="ENSGAGP00000021565.1"/>
        <property type="gene ID" value="ENSGAGG00000015822.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000005">
        <property type="entry name" value="leucine-rich repeat transmembrane neuronal protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019378821" evidence="13">
        <location>
            <begin position="34"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="516" mass="59305" checksum="DDC25D47776B1B68" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2E9</accession>
    <name>A0A452I2E9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein NDRG2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I2E9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021676"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024694.1">
        <property type="protein sequence ID" value="ENSGAGP00000021676.1"/>
        <property type="gene ID" value="ENSGAGG00000015907.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000034">
        <property type="entry name" value="NDRG2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019446808" description="Protein NDRG2" evidence="10">
        <location>
            <begin position="21"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="362"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="375"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="399"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="410" mass="44342" checksum="B3EE386977FA6178" modified="2019-05-08" version="1">MAKGPDAWVLSLALGGGAGAAMTELHEVQITEEKPLLLPPLQPIPGKKHSVETPYGLVAFTVHGTPKPKRPAILTYHDVGLNHQSCFETLFRFEDMQEIVKNFVVVHVDAPGMEEGAPLFPLGYQYPSLDQLADMIPCILQYLNFTSIIGIGVGAGAYILARYSLSHPDTVEGLVLINIDPNAKGWMDWAAHKLSGLTSSIPEMILAHLFSQEELSSCTELVQQYREALVGAPNLSNIQLYWSSYNTRRDLNFERGGDVTLKCPVMLVVGDQAPHEDAVVECNSKLDPTQTSFLKMADSGGQPQLTQPGKLTEAFKYFVQGMGYIPYVLDRKLSMGGLLLHDAPVALPHRLAVQRCLRGGEPVPLPHPVPEQRVGEPPAAATAGPGERSPHHGGLLLSRGGGGDGDASRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4S7</accession>
    <name>A0A452I4S7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA excision repair protein ERCC-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERCC1/RAD10/SWI10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I4S7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022545"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025685.1">
        <property type="protein sequence ID" value="ENSGAGP00000022545.1"/>
        <property type="gene ID" value="ENSGAGG00000016537.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070522">
        <property type="term" value="C:ERCC4-ERCC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032204">
        <property type="term" value="P:regulation of telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20079">
        <property type="entry name" value="XPF_nuclease_ERCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000017">
        <property type="entry name" value="DNA excision repair protein ERCC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000001">
        <property type="entry name" value="DNA excision repair protein ERCC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047260">
        <property type="entry name" value="ERCC1-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004579">
        <property type="entry name" value="ERCC1/RAD10/SWI10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00597">
        <property type="entry name" value="rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749:SF0">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749">
        <property type="entry name" value="EXCISION REPAIR CROSS-COMPLEMENTING 1 ERCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03834">
        <property type="entry name" value="Rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC1-like central" evidence="10">
        <location>
            <begin position="123"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="67"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="320" mass="34615" checksum="24AEB83740BFF149" modified="2019-05-08" version="1">MEPMEAAQAPEGRKRFTVQSQDPEHTMVRPIFKSSCSAAEPRAPALPGATSYAEYVVAQVSASSASMPVSRPARMTAASSGSREGAASGALLPSTEANTVPPAASGDSDPSPTLKLGAKSNCIIVSARQRGNPILKFVRNVPWEFGEIIPDYVLGQSTCALFLSLRYHNLNPNYIHARLQLLGKTYAVQVLLVQVDVKDPHQALKELAKMCILADCTLILAWSPEEAGRYLETYKAYEQKPADLLKEKVDQDFLSIVTDCLTSVKSVNKTDALTLLSTFGSLANIAQASKEDLSLCPGIGPQKAKRLFDTLHEPFLKIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I527</accession>
    <name>A0A452I527_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc finger protein 777</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I527"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022629"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025781.1">
        <property type="protein sequence ID" value="ENSGAGP00000022629.1"/>
        <property type="gene ID" value="ENSGAGG00000016594.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000151">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000250">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001530">
        <property type="entry name" value="Zinc finger protein 268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001271">
        <property type="entry name" value="Zinc finger protein 282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF158">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="128"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="50421" checksum="B89766D607858E95" modified="2019-05-08" version="1">RLVPGAAPQLHPGFDTRPADHHATEISLWTVVAAIQAVERKVNSHATQLLNLEGRTGTTEKKLTDCERTAMEFGNQLESKWTILGTLIQENNFLQRRLENMENLLRNRNFWILRLPPGTKGEVPKVPLTFDDVSVYFNEQEWENLEEWQKELYKNVMKGNYETLISLEEEKPQEGCLEKLKPKEEVLQSLQQGVAAQLSNPAVIRMEQSTQCGRDFSLLKGFVVQPGEKPFACSECGKSFRLKGNLVKHLRSHAKVRPYKCSECEKSFNCQSELLRHQMIHRGEKPYKCSECEKSYSRKVYLLNHQRVHTGERPFQCTVCEKSFRQKAVLISHQRLHSGERPFKCTECDNSFSEKSKLNNHYRIHTGEWPYKCTKCDKSYSRKVYLLNHQRLHTGERPFQCTACSKSFMLKTSFMKGTYCYIAASLQVSATAFRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8P7</accession>
    <name>A0A452I8P7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MANSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I8P7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024042"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027383.1">
        <property type="protein sequence ID" value="ENSGAGP00000024042.1"/>
        <property type="gene ID" value="ENSGAGG00000017590.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008544">
        <property type="term" value="P:epidermis development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013980">
        <property type="entry name" value="MANSC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011106">
        <property type="entry name" value="MANSC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46750">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46750:SF2">
        <property type="entry name" value="MANSC DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07502">
        <property type="entry name" value="MANEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00765">
        <property type="entry name" value="MANEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50986">
        <property type="entry name" value="MANSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019412407" description="MANSC domain-containing protein" evidence="6">
        <location>
            <begin position="18"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="MANSC" evidence="7">
        <location>
            <begin position="37"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="347" mass="39067" checksum="A2BD1CFFDD97872E" modified="2019-05-08" version="1">MFLLVAVAEVLFVLGLAWKSDSLCSPTTFYKNCWIRRFPGLLIDLEESQKRGAQVLKIYTEVTAEQCSRTCCLLKNVSCNLAVFYLETIHENINCLHIYCPALESCILRARTNVILYNITTGIDPDLLVFEKLSYKDPNTRSSFNKWERQNSSRIADSEKCRHNNVTLRSLLLQYPPSTTSQGLVANGSHSHSTSGLIPNTKPITRSWARSLPLDDHFAKEMELTSESTGLTSNSDKKMSVSTKMLSHVPSPARLNISQQHFNETKGYSGRNYTSDNEGQRPAWVAVDTGAWFVPLVLCSSFIFLCCCTVLLATGRCRKRRGHYKPVQRGEGGSKQYIKYTLVKDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9M3</accession>
    <name>A0A452I9M3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the Deltex family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452I9M3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024242"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027598.1">
        <property type="protein sequence ID" value="ENSGAGP00000024242.1"/>
        <property type="gene ID" value="ENSGAGG00000017723.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09633">
        <property type="entry name" value="Deltex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039396">
        <property type="entry name" value="Deltex_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039399">
        <property type="entry name" value="Deltex_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039398">
        <property type="entry name" value="Deltex_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048418">
        <property type="entry name" value="DTX3L_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048409">
        <property type="entry name" value="DTX3L_KH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057051">
        <property type="entry name" value="PARP14_RPM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12622">
        <property type="entry name" value="DELTEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18102">
        <property type="entry name" value="DTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21717">
        <property type="entry name" value="DTX3L_a-b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21718">
        <property type="entry name" value="KH_DTX3L"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23222">
        <property type="entry name" value="RRM_PARP14_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="573"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="91"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="544"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="546"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="752" mass="84739" checksum="8DB8EC3CF745EF9A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB13</accession>
    <name>A0A452IB13_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Palmitoyl-protein thioesterase ABHD10, mitochondrial</fullName>
            <ecNumber evidence="7">3.1.1.93</ecNumber>
            <ecNumber evidence="3">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Acyl-protein thioesterase ABHD10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Alpha/beta hydrolase domain-containing protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Mycophenolic acid acyl-glucuronide esterase, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as an acyl-protein thioesterase that hydrolyzes fatty acids from acylated residues in proteins. Regulates the mitochondrial S-depalmitoylation of the nucleophilic active site residue of peroxiredoxin-5/PRDX5, a key antioxidant protein, therefore modulating mitochondrial antioxidant ability. Also catalyzes the deglucuronidation of mycophenolic acid acyl-glucuronide, an active metabolite of the immunosuppressant drug mycophenolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19234"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>mycophenolic acid O-acyl-beta-D-glucuronide + H2O = mycophenolate + D-glucuronate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:34179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="ChEBI" id="CHEBI:62932"/>
            <dbReference type="ChEBI" id="CHEBI:66982"/>
            <dbReference type="EC" id="3.1.1.93"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:34180"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.93" evidence="7"/>
    <dbReference type="EC" id="3.1.2.22" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452IB13"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024916"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028363.1">
        <property type="protein sequence ID" value="ENSGAGP00000024916.1"/>
        <property type="gene ID" value="ENSGAGG00000018208.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102390">
        <property type="term" value="F:mycophenolic acid acyl-glucuronide esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052382">
        <property type="entry name" value="ABHD10_acyl-thioesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16138">
        <property type="entry name" value="MYCOPHENOLIC ACID ACYL-GLUCURONIDE ESTERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16138:SF7">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE ABHD10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="15">
        <location>
            <begin position="147"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="401" mass="42923" checksum="E58B3D146FD7B1E1" modified="2019-05-08" version="1">MSTLSISGITPSDTYAASPAALQGRSEGLGYQARGCTSFCSRGARLLGTPACGASNPVCFICCSSAYCSPRPQPRSSSIFNVVPLEGGQAWPCWVYPLTFEVAPLLRAAHRERAVRPSSTMASPLFLSRGALPRLAYHRLAGRSPGIVFLSGFNDTRANPKCLALEQFSLHLCPLSFDYTGCGSSDGCFEDCLIGHWKNDVLTVLDELTSGPQILVGVSMGGWLMLLAALERPGRVAGLVATGIGADAFITLYRELTPEVKGEVEQTGVFNYVMAGARSVLLKREFFMEAEKHSVLGCPSIPIICPVRLLHGMQDQLVPWGRAVQVAEQLESQDVRITLFKDGQHALKGAEEVKVLLGMVRDLAESLQRELGLGERLEAAMGTEPADGAAVTRQGALSGPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB84</accession>
    <name>A0A452IB84_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 17">NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial</fullName>
            <ecNumber evidence="4 17">7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:29092"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Core subunit of respiratory chain NADH dehydrogenase (Complex I) which is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme. Interacts with RAB5IF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Mitochondrion inner membrane</location>
            <topology evidence="17">Peripheral membrane protein</topology>
            <orientation evidence="17">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="4 17"/>
    <dbReference type="AlphaFoldDB" id="A0A452IB84"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024994"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028454.1">
        <property type="protein sequence ID" value="ENSGAGP00000024994.1"/>
        <property type="gene ID" value="ENSGAGG00000017436.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.230:FF:000001">
        <property type="entry name" value="Mitochondrial NADH dehydrogenase flavoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.600:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11540:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050837">
        <property type="entry name" value="ComplexI_51kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011537">
        <property type="entry name" value="NADH-UbQ_OxRdtase_suF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054765">
        <property type="entry name" value="SLBB_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01959">
        <property type="entry name" value="nuoF_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010120">
        <property type="entry name" value="PRK13596.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE FLAVOPROTEIN 1 NDUFV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22461">
        <property type="entry name" value="SLBB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="Nqo1 FMN-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142984">
        <property type="entry name" value="Nqo1 middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="Nqo1C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00644">
        <property type="entry name" value="COMPLEX1_51K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="17" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 17" id="KW-0288">FMN</keyword>
    <keyword evidence="11 17" id="KW-0408">Iron</keyword>
    <keyword evidence="12 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13 17" id="KW-0520">NAD</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="17" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="17" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 51kDa subunit iron-sulphur binding" evidence="19">
        <location>
            <begin position="363"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU364066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="462" mass="50776" checksum="A086E8839F3691F1" modified="2019-05-08" version="1">RAHGLKQDGWSPKALSRRWGGHVTAPKKTQFGSLKDVDRIFTNLYGRHDWRLKGALSRGDWYKTKEILLKGVDWILNEIKTSGLRGRGGAGFPTGLKWSFMNKPSDGRPKYLVVNADEGEPGTCKDREIMRHDPHKLVEGCLVAGRSMGARAAYIYIRGEFYNEASNLQVAIREAYEAGLLGKDACSSGYDFDVFVVRGAGAYICGEETALIESIEGKQGKPRLKPPFPADVGVFGCPTTVANVETVAVAPTICRRGGAWFASFGRERNSGTKLFNISGHVNTPCTVEEEMSVPLKELIEKHAGGVRGGWDNLLAVIPGGSSTPLIPKSVCETVPMDFDGLVQAQTGLGTAAVIVMDKSTDIVRAIARLIEFYKHESCGQCTPCREGVDWMNKVMWRFVQGNAQAAEIDALWEISKQIEGHTICALGDGAAWPVQGLIRHFRPELEERMRRHEETQARQASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBL7</accession>
    <name>A0A452IBL7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHHC U11-48K-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IBL7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024918"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028365.1">
        <property type="protein sequence ID" value="ENSGAGP00000024918.1"/>
        <property type="gene ID" value="ENSGAGG00000018216.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051591">
        <property type="entry name" value="UPF0224_FAM112_RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21402:SF5">
        <property type="entry name" value="GAMETOCYTE SPECIFIC FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21402">
        <property type="entry name" value="GAMETOCYTE SPECIFIC FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="CHHC U11-48K-type" evidence="4">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="115" mass="13273" checksum="85181F00FD2B24E6" modified="2019-05-08" version="1">MDPEQLIPCPYDKNHQIRASSFLYHLVKCRENNKKRAKELATCPYNARHRVPKRELQLHISACTDNRASELFSEPVGNFFCQKTLCFTDLHEHLMGKGSLGFDTHCNLENLSLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ID22</accession>
    <name>A0A452ID22_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Hexosyltransferase</fullName>
            <ecNumber evidence="16">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14389"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:133507"/>
            <dbReference type="ChEBI" id="CHEBI:134090"/>
            <dbReference type="EC" id="2.4.1.149"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with B3GNT8; this interaction greatly increases B3GNT2 catalytic activity, independently of B3GNT8 enzymatic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Golgi apparatus membrane</location>
            <topology evidence="2 16">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A452ID22"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025417"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028925.1">
        <property type="protein sequence ID" value="ENSGAGP00000025417.1"/>
        <property type="gene ID" value="ENSGAGG00000018584.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008532">
        <property type="term" value="F:N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016262">
        <property type="term" value="F:protein N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030311">
        <property type="term" value="P:poly-N-acetyllactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000010">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF87">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019330115" description="Hexosyltransferase" evidence="17">
        <location>
            <begin position="24"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="405" mass="44739" checksum="772FACEB66590617" modified="2019-05-08" version="1">PTCSTCCLLTVALALLTLLALKAYMDWSLEQPEGLLPAEPAWPTTPDPLAEPLGDDPAGLPLQLNASLQRLRSTVPEADAYWNRQQHGLFRALEGGPANGTRGCGDGPAAAAEISDFASYPEQHRRFVLHAECRSFPLLVNQPHKCAGNRTFLLLAIKSVPGNFAARQAVRETWGREGEHGGRPVRTLFLMGAAHGQHQPDLRRLVAYESQAYGDVLVWDFEDTFFNLTLKDVLFLGWVLAHCPSARFVLKGDDDVFINTPRVLAYLGALPGRRAQALYMGQVMAHAAPFRQASSKYYIPESFYAGPYPAYAGGGGYIFSGRLAPALFLVAQHVALYPIDDVYAGLCFHALGVRAEAHPAFQTFDIAEKDRADPCAHRQLFLVHRRSPQQTLRLWRQLHDPQLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IE06</accession>
    <name>A0A452IE06_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20 21">Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase</fullName>
            <shortName evidence="21">GNT-I</shortName>
            <shortName evidence="21">GlcNAc-T I</shortName>
            <ecNumber evidence="17 21">2.4.1.101</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 21">N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16 21">Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 21">
            <text>N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + UDP-N-acetyl-alpha-D-glucosamine = N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11456"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="Rhea" id="RHEA-COMP:14368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60625"/>
            <dbReference type="EC" id="2.4.1.101"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="21">The cofactor is mostly bound to the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 21">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 21">Golgi apparatus membrane</location>
            <topology evidence="1 21">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the glycosyltransferase 13 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.101" evidence="17 21"/>
    <dbReference type="AlphaFoldDB" id="A0A452IE06"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025930"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029497.1">
        <property type="protein sequence ID" value="ENSGAGP00000025930.1"/>
        <property type="gene ID" value="ENSGAGG00000018939.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003827">
        <property type="term" value="F:alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02514">
        <property type="entry name" value="GT13_GLCNAC-TI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.20:FF:000001">
        <property type="entry name" value="alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000055">
        <property type="entry name" value="Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.20">
        <property type="entry name" value="N-Acetylglucosaminyltransferase I, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004139">
        <property type="entry name" value="Glyco_trans_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052261">
        <property type="entry name" value="Glycosyltransferase_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10468:SF0">
        <property type="entry name" value="ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10468">
        <property type="entry name" value="PROTEIN O-LINKED-MANNOSE BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE 1/ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03071">
        <property type="entry name" value="GNT-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 21" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 21" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 21" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 21" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00069743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU368119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="453" mass="52718" checksum="B914DFADCA2D5C5B" modified="2019-05-08" version="1">MLKKSSLVLWGVVLFVAWNALLLFFLWTRPPAFDNHSHLTEEVIRLAQDAELELEHQKDLLRQIHRYSALWSKKRKKVEETGRLPLPAAASDTGALPATEVVLHRSRASPDAVLPVVVIACDRSTVRRCLDKLLHYRPSRERFPIIVSQDCGHEETARVIASYGDAVMHIKQPDLSDIPVPTDHRKFQGYYKIARHYRWALSQVFRTFQYQAAIVVEDDLEVAPDFFEYFQAAFPLLLADPSLWCVSAWNDNGKEQMVEAGRPELLYRTDFFPGLGWLLLSELWDELEPKWPRAFWDDWMRQPEQRRGRSCIRPEVSRTMTFGRKGVSHGQFFDQYLKFIKLNDRFVPFTQLDLSYLKKEEYERLFLPKVYDAPELRVEDLQGNQHRELGTVRVQYSSRDSFKAFAKALGVMDDLKSGVPRAGYRGIVSFLYRGRRVYLAPPSDWTGYDPSWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IER4</accession>
    <name>A0A452IER4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IER4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026410"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030022.1">
        <property type="protein sequence ID" value="ENSGAGP00000026410.1"/>
        <property type="gene ID" value="ENSGAGG00000019250.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20062">
        <property type="entry name" value="FH_FOXO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000032">
        <property type="entry name" value="Forkhead box protein O4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047409">
        <property type="entry name" value="FH_FOXO4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032067">
        <property type="entry name" value="FOXO-TAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032068">
        <property type="entry name" value="FOXO_KIX-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45767">
        <property type="entry name" value="FORKHEAD BOX PROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45767:SF3">
        <property type="entry name" value="FORKHEAD BOX PROTEIN O4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16676">
        <property type="entry name" value="FOXO-TAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16675">
        <property type="entry name" value="FOXO_KIX_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="10">
        <location>
            <begin position="109"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="8">
        <location>
            <begin position="109"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="184"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="566" mass="60288" checksum="4ACCD742628B504F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFZ5</accession>
    <name>A0A452IFZ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">poly(ADP-ribose) glycohydrolase</fullName>
            <ecNumber evidence="2">3.2.1.143</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the poly(ADP-ribose) glycohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.143" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452IFZ5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026670"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030312.1">
        <property type="protein sequence ID" value="ENSGAGP00000026670.1"/>
        <property type="gene ID" value="ENSGAGG00000019416.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
        <property type="term" value="F:poly(ADP-ribose) glycohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990966">
        <property type="term" value="P:ATP generation from poly-ADP-D-ribose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046372">
        <property type="entry name" value="PARG_cat_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048362">
        <property type="entry name" value="PARG_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007724">
        <property type="entry name" value="Poly_GlycHdrlase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837">
        <property type="entry name" value="POLY ADP-RIBOSE GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837:SF15">
        <property type="entry name" value="POLY(ADP-RIBOSE) GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05028">
        <property type="entry name" value="PARG_cat_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20811">
        <property type="entry name" value="PARG_cat_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PARG helical" evidence="8">
        <location>
            <begin position="589"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="PARG catalytic Macro" evidence="7">
        <location>
            <begin position="713"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="189"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="278"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="321"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="340"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="744"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="762"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="763"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="747"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="761"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="802"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR607724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR607724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="985" mass="111240" checksum="390711DDC291C399" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHG8</accession>
    <name>A0A452IHG8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Heparan-sulfate 6-O-sulfotransferase</fullName>
            <ecNumber evidence="9">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>alpha-D-glucosaminyl-[heparan sulfate](n) + 3'-phosphoadenylyl sulfate = 6-sulfo-alpha-D-glucosaminyl-[heparan sulfate](n) + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56604"/>
            <dbReference type="Rhea" id="RHEA-COMP:9830"/>
            <dbReference type="Rhea" id="RHEA-COMP:14621"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:58388"/>
            <dbReference type="ChEBI" id="CHEBI:140604"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sulfotransferase 6 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452IHG8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027355"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031074.1">
        <property type="protein sequence ID" value="ENSGAGP00000027355.1"/>
        <property type="gene ID" value="ENSGAGG00000019890.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017095">
        <property type="term" value="F:heparan sulfate 6-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000852">
        <property type="entry name" value="Heparan-sulfate 6-O-sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010635">
        <property type="entry name" value="Heparan_SO4-6-sulfoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12812">
        <property type="entry name" value="HEPARAN SULFATE 6-O-SULFOTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12812:SF6">
        <property type="entry name" value="HEPARAN-SULFATE 6-O-SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="191"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="195"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="233" mass="27871" checksum="D82B5A09E1A1EA91" modified="2019-05-08" version="1">RNFYYITILRDPVSRYLSEWRHVQRGATWKASLHVCDGRSPTTEELPSCYTGDDWSGCSLKEFMDCPYNLANNRQVRMLSDLSLVGCYNLSVMPEEQRNKVLLDSAKENLKRMAFFGLTEFQRKTQYLFEKTFNMNFIAPFTQYNSTRASSVEIDEQTQNHIETLNFLDMELYDYAKDLFLQRYQYMRQKEHQEARRKRQEQRKILKAKQAHNKEQSDDNSTTDYIGNVERWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHI8</accession>
    <name>A0A452IHI8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IHI8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026928"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030604.1">
        <property type="protein sequence ID" value="ENSGAGP00000026928.1"/>
        <property type="gene ID" value="ENSGAGG00000019605.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000380">
        <property type="entry name" value="CTD small phosphatase-like protein isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="119" mass="13809" checksum="C512FB6461EE2AD5" modified="2019-05-08" version="1">MGELFECVLFTASLAKYADPVADLLDRWGVFRARLFRESCVFHRGNYVKDLSRLGRELSKVIIVDNSPASYIFHPENAVPVQSWFDDMTDTELLDLIPFFEGLSREEEVYSMLHKLCNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJ54</accession>
    <name>A0A452IJ54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ADAM metallopeptidase domain 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJ54"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031710.1">
        <property type="protein sequence ID" value="ENSGAGP00000027919.1"/>
        <property type="gene ID" value="ENSGAGG00000020274.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002693">
        <property type="term" value="P:positive regulation of cellular extravasation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051044">
        <property type="term" value="P:positive regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022407">
        <property type="term" value="P:regulation of cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000002">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018358">
        <property type="entry name" value="Disintegrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF20">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00289">
        <property type="entry name" value="DISINTEGRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00427">
        <property type="entry name" value="DISINTEGRIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019539859" description="ADAM metallopeptidase domain 8" evidence="11">
        <location>
            <begin position="22"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="680"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="14">
        <location>
            <begin position="203"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="13">
        <location>
            <begin position="411"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="635"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="753"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="811"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="469"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="639"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="657"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="869" mass="94661" checksum="F95C72BEC5817D11" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILK6</accession>
    <name>A0A452ILK6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Integrin alpha-V/beta-5 (ITGAV:ITGB5) is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Heterodimer of an alpha and a beta subunit. Beta-5 (ITGB5) associates with alpha-V (ITGAV). Interacts with MYO10. Interacts with DAB2. Integrin ITGAV:ITGB5 interacts with FBLN5 (via N-terminus). ITGAV:ITGB5 interacts with CCN3. Interacts with tensin TNS3; TNS3 also interacts with PEAK1, thus acting as an adapter molecule to bridge the association of PEAK1 with ITGB5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 22">Cell membrane</location>
            <topology evidence="1 22">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 22">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILK6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028754"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032667.1">
        <property type="protein sequence ID" value="ENSGAGP00000028754.1"/>
        <property type="gene ID" value="ENSGAGG00000020556.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034684">
        <property type="term" value="C:integrin alphav-beta5 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043149">
        <property type="term" value="P:stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000006">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000043">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000075">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000258">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1510:FF:000021">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.30:FF:000001">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040622">
        <property type="entry name" value="EGF_integrin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057073">
        <property type="entry name" value="EGF_integrin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033760">
        <property type="entry name" value="Integrin_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014836">
        <property type="entry name" value="Integrin_bsu_cyt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012896">
        <property type="entry name" value="Integrin_bsu_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036349">
        <property type="entry name" value="Integrin_bsu_tail_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002369">
        <property type="entry name" value="Integrin_bsu_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057243">
        <property type="entry name" value="Integrin_I-EGF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF26">
        <property type="entry name" value="INTEGRIN BETA-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23105">
        <property type="entry name" value="EGF_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18372">
        <property type="entry name" value="I-EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08725">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00362">
        <property type="entry name" value="Integrin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17205">
        <property type="entry name" value="PSI_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002512">
        <property type="entry name" value="Integrin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00187">
        <property type="entry name" value="INB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01241">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01242">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69687">
        <property type="entry name" value="Integrin beta tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00243">
        <property type="entry name" value="I_EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52047">
        <property type="entry name" value="I_EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 22" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 22" id="KW-0401">Integrin</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 24" id="KW-0732">Signal</keyword>
    <keyword evidence="6 22 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019564660" description="Integrin beta" evidence="24">
        <location>
            <begin position="17"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="743"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="26">
        <location>
            <begin position="53"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit VWA" evidence="25">
        <location>
            <begin position="61"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit tail" evidence="28">
        <location>
            <begin position="661"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit cytoplasmic" evidence="27">
        <location>
            <begin position="766"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="65"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="285"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="427"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="459"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="487"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="510"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="515"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="526"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="554"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="556"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="561"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="576"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="595"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="597"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="602"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="613"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="634"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="636"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="641"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="654"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="661"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="667"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="688"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00059582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00063697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR002512-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="SMART" id="SM00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="SMART" id="SM01241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="SMART" id="SM01242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="822" mass="91139" checksum="B7D9E652BD4513B0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IN07</accession>
    <name>A0A452IN07_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">FAD-dependent oxidoreductase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Required for the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). Involved in mid-late stages of complex I assembly.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IN07"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029240"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033212.1">
        <property type="protein sequence ID" value="ENSGAGP00000029240.1"/>
        <property type="gene ID" value="ENSGAGG00000021155.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.9.10:FF:000155">
        <property type="entry name" value="FAD-dependent oxidoreductase domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF287">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="16">
        <location>
            <begin position="82"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="440" mass="49000" checksum="F6A0425A9D12EB90" modified="2019-05-08" version="1">MIRAGSLCRGLRLLRALPLPGPALGCAPPSRRLRTGHPQRRDLFSDLEKEVVKFRKKLKETLPGSDWDPLGTTKGLPPERADVVIVGGGVMGWSVAYWLKALEPKRDALRVVVIEKDPTYSRASTVLSAGGIRQQFSLPENIQMSLYSAFFLRTINEHLGVVNEPPIDIQFNPSGYLFLASEDEAAKLENNVRIQRAEGAEVSLLSPAQLKKKYPWINTDGVAVASYGLENEGWFDPWSLLNAFRRKAMSMGVYQCFGEVISFTISSREIMVPEGELTFSHINYVRVQMPNSLEYQPVECSLVVNAAGPWSQKLAEMAGVGIGPPDTPEGIKLPVEPRKRYIFVWHCPDGPGLDCPMLVDATGTYFRREGLGGNYLGGLSPAEEEEPDIQDLEVDHDFFQEKIWPCLAHRVPVFESLKVKSAWAGYYDYNTFDQNAVLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IN90</accession>
    <name>A0A452IN90_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="20">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19713"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:19714"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(E)-hexadec-2-enoate + ATP + CoA = (2E)-hexadecenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36139"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:72745"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:36140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + ATP + CoA = 12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52112"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:90718"/>
            <dbReference type="ChEBI" id="CHEBI:136408"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:52113"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + ATP + CoA = 15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52116"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:78832"/>
            <dbReference type="ChEBI" id="CHEBI:136409"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:52117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = 5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65341"/>
            <dbReference type="ChEBI" id="CHEBI:136407"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:52109"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>hexadecanoate + ATP + CoA = hexadecanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30751"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:30752"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A452IN90"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029423"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033412.1">
        <property type="protein sequence ID" value="ENSGAGP00000029423.1"/>
        <property type="gene ID" value="ENSGAGG00000021268.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047676">
        <property type="term" value="F:arachidonate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000006">
        <property type="entry name" value="long-chain-fatty-acid--CoA ligase 6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF54">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 20" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4 20" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="22">
        <location>
            <begin position="143"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00024565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU369030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="724" mass="81362" checksum="0B36B8332742437C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452INR7</accession>
    <name>A0A452INR7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452INR7"/>
    <dbReference type="SMR" id="A0A452INR7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029544"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033549.1">
        <property type="protein sequence ID" value="ENSGAGP00000029544.1"/>
        <property type="gene ID" value="ENSGAGG00000021344.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000101">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="147" mass="16735" checksum="C942BE7853CBC355" modified="2019-05-08" version="1">MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPB9</accession>
    <name>A0A452IPB9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">small monomeric GTPase</fullName>
            <ecNumber evidence="2">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452IPB9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029685"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033706.1">
        <property type="protein sequence ID" value="ENSGAGP00000029685.1"/>
        <property type="gene ID" value="ENSGAGG00000021430.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051065">
        <property type="entry name" value="Ras-related_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45704">
        <property type="entry name" value="RAS-LIKE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="218" mass="24708" checksum="40D846DDF97ADDD3" modified="2019-05-08" version="1">MSFSRPLRRSVSLSQGRTLRLVVLGQGAVGKTEMIYRHTAVIDGEMVHFEILDTAGQEEDSLQIEEKIKWGDGFAVVYSVTDRCSFDEVMRLCFLINHIHSSPKRSGGSDQPPVVIVGNKKDLQFDRMVSCEDGENLSKALKLPFYEISTRDSYEETVVVFNTLYNELMRQGHFSPGSFKRRTVSKLMEKIPKMHANSTLNSGGRSLSFNSFRDYIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQR5</accession>
    <name>A0A452IQR5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IQR5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030451"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034556.1">
        <property type="protein sequence ID" value="ENSGAGP00000030451.1"/>
        <property type="gene ID" value="ENSGAGG00000021929.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="237"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="507" mass="57957" checksum="8343CAC9AA0E6B61" modified="2019-05-08" version="1">MDFRKVDFGKLRELIGKVPWESRLRGKTTEESWQFFKGTLLRAQKQAIPMVRKDRKCGKRPPWLNHEILRDLQNKKASYKKWKLGQITKDEYRQITQECRGKIRKAKAQNELKLAMGIKGNKKTFYQYIRSKRKTKDRVGPLLSEEGETVTGDLEMAEMLNDFFVSVFTEKSEGMSNIVNAYGKRVGLEDKIKKEQVKNHLEKLDACKSPGPDEMHPRILKELIEEVSEPLAIIFGKSWETGEIPEDWKGANIVPIYKKGNKNNPGNYRPVSLTSVPGKIMEQVIKEIICKHLEGGKVIGNSQHGFVKNKSCQTNLIAFFDRITSLVDKGEAVDVIYLDFSKAFDTVSHDILIDKLGKYNLDGATIRWVHNWLDNRTQRVVVNGSQSCWKGITSGVPQGSVLGPVLFNIFINDLDVGIESTLIKFADDTKLGGIATALEDRVKIQNDLDKLEKWSEVNRMKFNKDKCKVLHLGRNNQFHTYRMGRDCLGRSMAERDLGVIVDHKLNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRQ0</accession>
    <name>A0A452IRQ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Kelch domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of a CRL2(KLHDC2) E3 ubiquitin-protein ligase complex, also named ECS(KLHDC2) complex, composed of CUL2, Elongin BC (ELOB and ELOC), RBX1 and substrate-specific adapter KLHDC2. May form oligomers as a KLHDC2-ELOB-ELOC complex; this interaction is autoinhibitory for the E3 ligase complex as the substrate-binding site of KLHDC2 is blocked in the oligomer. Interacts with CREB3; interaction is direct and specific as it does not interact with CREB1, ATF4, ATF6, JUN, FOS, CEBPA or herpes simplex virus transactivator VP16.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IRQ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030532"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034648.1">
        <property type="protein sequence ID" value="ENSGAGP00000030532.1"/>
        <property type="gene ID" value="ENSGAGG00000021987.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140627">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the C-end degron rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000012">
        <property type="entry name" value="Kelch domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000043">
        <property type="entry name" value="Kelch domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46228">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46228:SF3">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="407" mass="46052" checksum="012A9C58B50B6CD4" modified="2019-05-08" version="1">MADDNEELQADEELPAPAEDNFEQLENNSPAERSGHVAVTDGHCMFVWGGYKNAQVRGFYDFYLPRDEIWIYSMETGRWNKKKTEGDVPPSMSGSCAVCVDKVLYLFGGHHARGNTNKFYMLNSRSKEKVLQWVRVECQGVPPSSKDKLGVWVYKNKLIFFGGYGYSPEGIPIGTFEFDETSFWNSGQPRGWNDHVHVLDTETFSWSQPITTGKSPSPRAAHACATVGNRGYVFGGRYRDSRMNDLYHLNLDTWEWTEIITQGVCPVGRSWHSLTPVSSDHLFLFGGFTTDKQPLSDAWIYCISKNEWKQFEHNYSEKPRLWHTACASEEGEVIVFGGCANNLLAHHKAAHSNEILVFSLQPKSLVRLCLEAVICFKEILANSWTCLPKHLLHSVNQRFGSNNTSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRV5</accession>
    <name>A0A452IRV5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Probable zinc metalloprotease.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M54 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IRV5"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034704.1">
        <property type="protein sequence ID" value="ENSGAGP00000030583.1"/>
        <property type="gene ID" value="ENSGAGG00000022024.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11375">
        <property type="entry name" value="Peptidase_M54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052009">
        <property type="entry name" value="Archaemetzincin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012962">
        <property type="entry name" value="Pept_M54_archaemetzincn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32205:SF4">
        <property type="entry name" value="ARCHAEMETZINCIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32205">
        <property type="entry name" value="ARCHAEMETZINCIN-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="593" mass="66727" checksum="9D9A1A7ECCEE3486" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ISC3</accession>
    <name>A0A452ISC3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Complement component C9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Pore-forming component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Constitutes the pore-forming subunit of the MAC complex: during MAC assembly, C9 associates with the C5b8 intermediate complex, and polymerizes to complete the pore.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homooligomer; about 20 C9 chains oligomerize to give rise to a huge beta-barrel that forms a 100 Angstrom diameter pore in target membranes. Component of the membrane attack complex (MAC), composed of complement C5b, C6, C7, C8A, C8B, C8G and multiple copies of the pore-forming subunit C9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ISC3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030828"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034961.1">
        <property type="protein sequence ID" value="ENSGAGP00000030828.1"/>
        <property type="gene ID" value="ENSGAGG00000022159.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005579">
        <property type="term" value="C:membrane attack complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044218">
        <property type="term" value="C:other organism cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006957">
        <property type="term" value="P:complement activation, alternative pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000001">
        <property type="entry name" value="semaphorin-5A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001862">
        <property type="entry name" value="MAC_perforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF3">
        <property type="entry name" value="COMPLEMENT COMPONENT C9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00764">
        <property type="entry name" value="COMPLEMENTC9"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0179">Complement alternate pathway</keyword>
    <keyword evidence="13" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="11" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="16 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0473">Membrane attack complex</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-1052">Target cell membrane</keyword>
    <keyword evidence="19" id="KW-1053">Target membrane</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="MACPF" evidence="24">
        <location>
            <begin position="131"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="544"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00093294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00093512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="571" mass="64522" checksum="770E5DE1DE5A1159" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ISD4</accession>
    <name>A0A452ISD4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Endonuclease V</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine. Active against both single-stranded and double-stranded RNAs. Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs). Cleaves mRNAs and tRNAs containing inosine. Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3'. Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing. Binds branched DNA structures.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Interacts with PABPC1; the interaction is RNA-dependent and stimulates ENDOV activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the endonuclease V family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ISD4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030777"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034906.1">
        <property type="protein sequence ID" value="ENSGAGP00000030777.1"/>
        <property type="gene ID" value="ENSGAGG00000022130.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06559">
        <property type="entry name" value="Endonuclease_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2170.10:FF:000002">
        <property type="entry name" value="Endonuclease V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007581">
        <property type="entry name" value="Endonuclease-V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511:SF1">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04493">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="269" mass="29931" checksum="7741C3CBDA2AB2D2" modified="2019-05-08" version="1">MARRETEPPEQETLRRWEREQAQLKANVIEEDTEEWQKDSTFAGLERVGGVDLSYVKGNDTVACASLVVLSYPELEVLYEDCQMVTVSAPYLAGYLAFREVPFLVEAAQRLQEREPGLRPQVLLVDGNGVLHHRAGFGVACHLGVLTGLPCIGVAKNLLQVDGLAKDEQHKGQIRDLQMGGDVFPLRGTSGRVLGMALRSYNKSTKPIYVSVGHRTCLESAVRLVQSCCRYRIPEPIRQVRRQGQLGGEDPSLSWETWPVREGVTGICE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ISU0</accession>
    <name>A0A452ISU0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ovomucoid</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ISU0"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035377.1">
        <property type="protein sequence ID" value="ENSGAGP00000031192.1"/>
        <property type="gene ID" value="ENSGAGG00000022418.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005520">
        <property type="term" value="F:insulin-like growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011390">
        <property type="entry name" value="IGFBP_rP_mac25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14186">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14186:SF14">
        <property type="entry name" value="KAZAL-TYPE SERINE PROTEASE INHIBITOR DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00050">
        <property type="entry name" value="Kazal_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018239">
        <property type="entry name" value="IGFBP_rP_mac25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="IGFBP N-terminal" evidence="7">
        <location>
            <begin position="24"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="8">
        <location>
            <begin position="94"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="145"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27803" checksum="46995A04EBF4A73A" modified="2019-05-08" version="1">MPERALAEGQKMQNSLSWLRLLREGDSCGKCNLELCSKPTHCPAGTVLDQCGCCPECGNVEGQICDLDKVNHFYGQCGENLECRLDADETKFGEIPEPQCVCKSQESVCGPEGKTYANICQFNEAYSEKRRNINMKHKGPCESAPVISLPPQDAQNFTGNDIIFGCEVSAYPMPHLEWKKKGNKMFLPGDDAHISIQARGGPKKYGVTGWLQIQGIKKSDEGIYICQTKNKYGIAYASARLKVIDGKVFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITC7</accession>
    <name>A0A452ITC7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Histone H4 transcription factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Histone nuclear factor P</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">MBD2-interacting zinc finger protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Methyl-CpG-binding protein 2-interacting zinc finger protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Transcriptional repressor that binds to the consensus sequence 5'-CGGACGTT-3' and to the RB1 promoter. Transcriptional activator that promotes histone H4 gene transcription at the G1/S phase transition in conjunction with NPAT. Also activates transcription of the ATM and PRKDC genes. Autoregulates its expression by associating with its own promoter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Binds MBD2 and a histone deacetylase complex. Interacts with NPAT.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ITC7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031296"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035497.1">
        <property type="protein sequence ID" value="ENSGAGP00000031296.1"/>
        <property type="gene ID" value="ENSGAGG00000022490.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002166">
        <property type="entry name" value="Histone H4 transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002279">
        <property type="entry name" value="Histone H4 transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051574">
        <property type="entry name" value="ZnF_E-box_Homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24391:SF26">
        <property type="entry name" value="HISTONE H4 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24391">
        <property type="entry name" value="HISTONE H4 TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="162"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="248"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="305"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="338"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="439"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00055361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="546" mass="63448" checksum="94E59CA33E3D7F0E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITV7</accession>
    <name>A0A452ITV7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RAF proto-oncogene serine/threonine-protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Raf-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452ITV7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031406"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035622.1">
        <property type="protein sequence ID" value="ENSGAGP00000031406.1"/>
        <property type="gene ID" value="ENSGAGG00000022566.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20870">
        <property type="entry name" value="C1_A_C-Raf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17135">
        <property type="entry name" value="RBD_CRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14149">
        <property type="entry name" value="STKc_C-Raf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000015">
        <property type="entry name" value="B-Raf proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000024">
        <property type="entry name" value="B-Raf proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000004">
        <property type="entry name" value="B-Raf proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000036">
        <property type="entry name" value="RAF proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003116">
        <property type="entry name" value="RBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF22">
        <property type="entry name" value="RAF PROTO-ONCOGENE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02196">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00455">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50898">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RBD" evidence="23">
        <location>
            <begin position="56"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="22">
        <location>
            <begin position="138"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="369"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="237"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="304"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="239"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="667" mass="75360" checksum="B34A5DC634446F30" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUF7</accession>
    <name>A0A452IUF7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM53 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IUF7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031750"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036005.1">
        <property type="protein sequence ID" value="ENSGAGP00000031749.1"/>
        <property type="gene ID" value="ENSGAGG00000022806.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000036006.1">
        <property type="protein sequence ID" value="ENSGAGP00000031750.1"/>
        <property type="gene ID" value="ENSGAGG00000022806.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029356">
        <property type="entry name" value="FAM53"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28567:SF2">
        <property type="entry name" value="PROTEIN FAM53A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28567">
        <property type="entry name" value="PROTEIN FAM53A-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15242">
        <property type="entry name" value="FAM53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="198"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="456" mass="51014" checksum="078D75D5805E2026" modified="2019-05-08" version="1">MVTLITEKLQNQSLDDLTCKTYNIHLYSSEKLNKSGSLFPFEINEESPWKALNGGCPIQADATKNSAYPFPVCPFSRGTANNVSLQWQQESSSTSMVSGWISELNLNENSGQPLAPPTKRHCRSLSEPDELARCRSPWKPSSSKVWTPVSKRRCNSGGSATLQRCNSHGSANLQRSTSISLPQNVLSLNNVFTITSFNTSPVPRPSSASSGFVDSSEGSTSSSIRWNSGGPCDFNPRRRLSLSQEHITETGNLLPSANSTPTSTPELSRRQGLLRCRSQPCVLNEKKSRLKRRREEDVRWNRPSLDFFKMTRTLTTSCFKQTSLNRAPHIIWKNELELVYMEKFFHKKTLKNSKSLCSLDYEEDDDDDDTQMKTIVSSPCDSHDFMNITPGSSPVKEQLDEIRHHGPCQVAFSVRDYKKAAAVCESDEDTSDCDSTEEGIFPLDCGDLDLEQIENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUX0</accession>
    <name>A0A452IUX0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IUX0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031670"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035919.1">
        <property type="protein sequence ID" value="ENSGAGP00000031670.1"/>
        <property type="gene ID" value="ENSGAGG00000022751.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="228"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="586" mass="67343" checksum="0F1DA5ECC88FEEFC" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IVZ9</accession>
    <name>A0A452IVZ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Lissencephaly-1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PAFAH1B1</name>
        <name evidence="14" type="synonym">LIS1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. May be required for proliferation of neuronal precursors and neuronal migration.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Can self-associate. Interacts with dynein, dynactin, NDE1 and NDEL1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
            <location evidence="14">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
            <location evidence="14">Cytoskeleton</location>
            <location evidence="14">Microtubule organizing center</location>
            <location evidence="14">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPI-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="14">Localizes to the plus end of microtubules and to the centrosome.</text>
    </comment>
    <comment type="domain">
        <text evidence="14">Dimerization mediated by the LisH domain may be required to activate dynein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the WD repeat LIS1/nudF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IVZ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032211"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036516.1">
        <property type="protein sequence ID" value="ENSGAGP00000032211.1"/>
        <property type="gene ID" value="ENSGAGG00000023060.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051012">
        <property type="term" value="P:microtubule sliding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000038">
        <property type="entry name" value="Lissencephaly-1 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.30:FF:000002">
        <property type="entry name" value="Platelet-activating factor acetylhydrolase ib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03141">
        <property type="entry name" value="lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017252">
        <property type="entry name" value="Dynein_regulator_LIS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037190">
        <property type="entry name" value="LIS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056795">
        <property type="entry name" value="PAC1-like_LisH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24951">
        <property type="entry name" value="LisH_PAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037647">
        <property type="entry name" value="Dynein_regulator_Lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109925">
        <property type="entry name" value="Lissencephaly-1 protein (Lis-1, PAF-AH alpha) N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 14" id="KW-0132">Cell division</keyword>
    <keyword evidence="11 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9 14" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 14" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 14" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10 14" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 14" id="KW-0813">Transport</keyword>
    <keyword evidence="4 15" id="KW-0853">WD repeat</keyword>
    <feature type="initiator methionine" description="Removed" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="PAC1-like LisH-like dimerisation" evidence="16">
        <location>
            <begin position="5"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="104"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="146"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="188"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="230"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="334"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="376"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF24951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="410" mass="46652" checksum="E88ADEFDF84D0D9E" modified="2019-05-08" version="1">MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDVNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFDCIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESSYSTISEATGSETKKSGKPGPFLLSGSRDKTIKMWDISTGMCLMTLVGHDNWVRGVLFHSGGKFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWW7</accession>
    <name>A0A452IWW7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Exportin-7/Ran-binding protein 17 TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IWW7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032411"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036746.1">
        <property type="protein sequence ID" value="ENSGAGP00000032411.1"/>
        <property type="gene ID" value="ENSGAGG00000023189.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000042">
        <property type="entry name" value="exportin-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057947">
        <property type="entry name" value="TPR_XPO7/RBP17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044189">
        <property type="entry name" value="XPO4/7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596">
        <property type="entry name" value="EXPORTIN 4,7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596:SF11">
        <property type="entry name" value="EXPORTIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25795">
        <property type="entry name" value="TPR_XPO7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-7/Ran-binding protein 17 TPR repeats" evidence="9">
        <location>
            <begin position="372"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1027" mass="116665" checksum="FFB81E8A89AFEF12" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYF4</accession>
    <name>A0A452IYF4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYF4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033114"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037535.1">
        <property type="protein sequence ID" value="ENSGAGP00000033114.1"/>
        <property type="gene ID" value="ENSGAGG00000023637.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052838">
        <property type="entry name" value="Myosin-XVI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029353">
        <property type="entry name" value="NYAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039482">
        <property type="entry name" value="NYAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47335">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-XVI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47335:SF1">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-XVI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15452">
        <property type="entry name" value="NYAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15439">
        <property type="entry name" value="NYAP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 6" id="KW-0518">Myosin</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="8">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="6">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="556"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="597"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="825"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="620"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="829"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="867"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="897"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="954"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1018"/>
            <end position="1055"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1163" mass="128717" checksum="EFA7800E38D1BBF4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZ95</accession>
    <name>A0A452IZ95_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Probable ATP-dependent RNA helicase DDX47</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DEAD box protein 47</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with AGO1 and AGO2. Interacts with GABARAP. Interacts with NOL8; the interaction is RNA-dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX47/RRP3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452IZ95"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033404"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037845.1">
        <property type="protein sequence ID" value="ENSGAGP00000033404.1"/>
        <property type="gene ID" value="ENSGAGG00000023801.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17954">
        <property type="entry name" value="DEADc_DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000626">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000681">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044765">
        <property type="entry name" value="DDX47/Rrp3_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF20">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="100"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="282"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="459"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="459"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="484"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="499" mass="55947" checksum="E8DA535818BF500E" modified="2019-05-08" version="1">MLCVRLAGPRDRPAPPRRHNTRCDVIGKRCFSGNHVSRRSPRWGSKVKWKMAAADAEQGPLEVEAEEPRSFKDLGVTDVLCETCDQLGWKTPTKIQVEAIPMALQGRDIIGLAETGSGKTGAFALPILQTLLETPQRLFALVLTPTRELAFQISEQFEALGSSIGVQSAVIVGGIDMMAQALTLAKKPHVIIATPGRLIDHLENTKGFNLRALKYLVMDEADRILNMDFETEVDKILKVIPRDRKTFLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQHYIFIPSRFKDSYLVYILNELAGNSFMIFCSTCNNTQRTALLLRNLGFTAISLHGQMSQNKRLGSLNKFKAKSRSILLATDVASRGLDVPHVDVVINFDIPTHSKDYIHRVGRTARAGRSGKSITFVTQYDVELFQRIEHLIGKKLPVFPTQEEEVMMLTERVAEAQRCARMELREQGERKKRSRDEANDDDTEGATGVRKKVAGGKMKKRKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZY4</accession>
    <name>A0A452IZY4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sphingomyelin phosphodiesterase 2</fullName>
            <ecNumber evidence="6">3.1.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Lyso-platelet-activating factor-phospholipase C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Neutral sphingomyelinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1-O-hexadecyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycerol + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36087"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:34115"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:36088"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1-O-octadecyl-sn-glycero-3-phosphocholine + H2O = 1-O-octadecyl-sn-glycerol + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39923"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74001"/>
            <dbReference type="ChEBI" id="CHEBI:75216"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:39924"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycerol + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:75542"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:41120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a sphingosylphosphocholine + H2O = a sphingoid base + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:84410"/>
            <dbReference type="ChEBI" id="CHEBI:85171"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:45297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>an N-(acyl)-sphingosylphosphocholine + H2O = an N-acyl-sphingoid base + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45300"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64583"/>
            <dbReference type="ChEBI" id="CHEBI:83273"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:45301"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the neutral sphingomyelinase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.12" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452IZY4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033624"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038089.1">
        <property type="protein sequence ID" value="ENSGAGP00000033624.1"/>
        <property type="gene ID" value="ENSGAGG00000023942.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000033">
        <property type="entry name" value="sphingomyelin phosphodiesterase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF24">
        <property type="entry name" value="PHOSPHODIESTERASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="24">
        <location>
            <begin position="27"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00068543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00075307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="408" mass="45939" checksum="C48EDA907F467799" modified="2019-05-08" version="1">MDTDPPLQLRVFNLNCWAIRYLSKLRQERIGLIGDTLRQEGFDLALLQEVWSERDYCELKQKLTACYPYSHYFKSGVIGSGLCVFSRYPILDTFLYQYSLNGYPYMLQHGDWFCGKAVGLLVIKICGIIFHVYVTHLHAEYCRDKDAYLPHRVVQAWELAQFIQHTSKGADVVLLGGDLNMHPGDVGIRLLRGWTGLRDSFPDTERFEGCEDGCTLVPTNCFINKEELQPFPLGIRIDYILYKGLSHFAVKCDSLVTTTGTAPGKSIPYSDHEAVMATLCVKPREEAKGSSSAIEPELVDVVNEARTEVRVGLHTAERQRYSNGRMAILALLLLLLQAAMGLSSLVGWDSPQPFPKLSFSLLSLLATVVLLLSMVVYVFHTIEVKMLQGTEEQMRLALQALQDKLSSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZZ4</accession>
    <name>A0A452IZZ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZZ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033705"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038177.1">
        <property type="protein sequence ID" value="ENSGAGP00000033705.1"/>
        <property type="gene ID" value="ENSGAGG00000023991.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990430">
        <property type="term" value="F:extracellular matrix protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051505">
        <property type="entry name" value="C-type_lectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789:SF5">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 14 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789">
        <property type="entry name" value="CHONDROLECTIN VARIANT CHODLFDELTAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019263909" description="C-type lectin domain-containing protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="10">
        <location>
            <begin position="32"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="301"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="376"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="309"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="431" mass="44957" checksum="4CE07128EDC77B0E" modified="2019-05-08" version="1">MRGAIPLCVLLHASSVLCRAEEPRGNYTWCEGSGACYSVHLAQVSFGRAQEACAVRGGALSTASGEAEVRAILALLRVVAAEPGSWVFWLGLVRRQQQCTQMEWPLRGFSWLAGPQQAQGEPKELDKWVKEPSRSCLTRRCAGLQVTAGRQDLEPWGWKERTCKGNTTQGYVCKYQYNGTCPAPRPQGARSLLYSLPYQLHSAALDFSPPGTELVVACAGPQGDVRLLCELGRGGYNWTGAGEQLCPCPSGYQSPSTGACMEPEDCTSAQGAFLCVCARGFLLGADKKSCVRPGHIGGSATAAPDPTLSAGGTVASSSSGAPSPPSSPQPSTAGEVQSSSSSPTYVFILVALAVVTVVILVGAVLVVFKLCFTKSPSSASGAGKEPAPAAAPESDPEATTTSSENSLEAQGDTAERLQGEPSPEEEAPPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J339</accession>
    <name>A0A452J339_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J339"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034827"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039422.1">
        <property type="protein sequence ID" value="ENSGAGP00000034827.1"/>
        <property type="gene ID" value="ENSGAGG00000024739.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009954">
        <property type="term" value="P:proximal/distal pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000018">
        <property type="entry name" value="Homeobox A10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006711">
        <property type="entry name" value="Hox9_activation_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017112">
        <property type="entry name" value="HXA9/HXB9/HXC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45970">
        <property type="entry name" value="AGAP004664-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45970:SF3">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04617">
        <property type="entry name" value="Hox9_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037109">
        <property type="entry name" value="Homeobox_Hox9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="193"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="195"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="147"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="261" mass="29813" checksum="ED58FB0DC21F8CC1" modified="2019-05-08" version="1">MSTSGTLSNYYVDSFIIHESEDLVQSRYASAPLAPPTRQPALSEHPEFTPCSFQSKTSVFSTSWNPVHPPSANNVPTVYHPYVHHQAPMAAPDGRYMRSWLEPMPGSLSFPGLPSSRHYGIKPEPLAARRGDCTTFDTHTLSLSDYACGSPPADRDKQPSEGAFSENNGENETNGEKPQIDPNNPAANWLHARSTRKKRCPYTKHQTLELEKEFLFNMYLTRDRRYEVARLLNLTERQVKIWFQNRRMKMKKINKDRAKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J3D4</accession>
    <name>A0A452J3D4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J3D4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034948"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039572.1">
        <property type="protein sequence ID" value="ENSGAGP00000034948.1"/>
        <property type="gene ID" value="ENSGAGG00000024846.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031937">
        <property type="entry name" value="PNISR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31518">
        <property type="entry name" value="ARGININE/SERINE-RICH PROTEIN PNISR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15996">
        <property type="entry name" value="PNISR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="516"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="618"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="639"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="742"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="764"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="781"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="799"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="824" mass="94685" checksum="B372EBE69A6E5E17" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J453</accession>
    <name>A0A452J453_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Anaphase-promoting complex subunit CDC26</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cell division cycle protein 26 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CDC26 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J453"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035213"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039873.1">
        <property type="protein sequence ID" value="ENSGAGP00000035213.1"/>
        <property type="gene ID" value="ENSGAGG00000025034.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018860">
        <property type="entry name" value="APC_suCDC26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28579">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT CDC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28579:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT CDC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10471">
        <property type="entry name" value="ANAPC_CDC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="85" mass="9879" checksum="48A79C84B3565793" modified="2019-05-08" version="1">MLRRKPTRLELKLDDIEEFESIRKDLESCKKQREDVDVVGATDGEGAIGLNNDYKSREQMINDRIGYKPQPKPNNRSSQFGSFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5E9</accession>
    <name>A0A452J5E9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J5E9"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040439.1">
        <property type="protein sequence ID" value="ENSGAGP00000035712.1"/>
        <property type="gene ID" value="ENSGAGG00000025358.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="80" mass="8496" checksum="40782881F56763B9" modified="2019-05-08" version="1">MPCHGKHGAHSAHRHCTSPGCCFQSLDISCNPRDTVDAHLLHPTSLYLLHTLAHNVRGSMGTMALATALGAMGQVLVLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J618</accession>
    <name>A0A452J618_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J618"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035940"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040682.1">
        <property type="protein sequence ID" value="ENSGAGP00000035940.1"/>
        <property type="gene ID" value="ENSGAGG00000025488.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02872">
        <property type="entry name" value="GH18_chitolectin_chitotriosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000007">
        <property type="entry name" value="Acidic mammalian chitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000001">
        <property type="entry name" value="Chitinase 3-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF188">
        <property type="entry name" value="ACIDIC MAMMALIAN CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="7">
        <location>
            <begin position="48"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="495" mass="54476" checksum="85A6C95287EB335F" modified="2019-05-08" version="1">MTGTNWRYLSPRLVNCLCILHFIQIVSLLLLFLTGLALLLNAQLGSSYLLTCYFTNWAQYRPGLGHFMPDNIDTCLCTHLIYAFAVMKNNQITTIEWNDLTLYKSFNGLKNQNGQLKTLLAIRGWNFGTPCSNLTPFPFFLPRFTAMVSTPETHQTFISSVITFLPQYEFDGIDFDWEYPGSRGSTAQDKGLFTVLEMLEAFEQEAKQVNKPRLLVTAAVAAGLSNIESGYQIPQLGQYLDYFHLMTYDFHGSWEGYTGENSPLFQGPADTGSNIYLNIEYAMNYWKNNGAPVEKLIVVFPTYGHTFILTDPSNTAVGAPTSGPGPAGAYTKQCGFWAYYEICTFLKNGATQAWDTPQDVSYAYQSTEWVGYDNIKSFNLKAQWLTKNNFGSAMVWTIDLDDFTGTFCNQGKFPLISTLKTALGLQSTMSCTAPAQPISPITEAPSGGGSGSGSSGSGSSSSGGSGFCAGKANGLYSVANNRNAFWNCVNGITYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J6W5</accession>
    <name>A0A452J6W5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ferritin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J6W5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036103"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040869.1">
        <property type="protein sequence ID" value="ENSGAGP00000036103.1"/>
        <property type="gene ID" value="ENSGAGG00000025591.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="47" mass="5366" checksum="6826CFB0CE534639" modified="2019-05-08" version="1">LNSASTSQVRQNFHAECEASVNHLVNLECYASYVYLCPTTLSRMMWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J701</accession>
    <name>A0A452J701_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J701"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036266"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041055.1">
        <property type="protein sequence ID" value="ENSGAGP00000036266.1"/>
        <property type="gene ID" value="ENSGAGG00000025697.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000645">
        <property type="entry name" value="Zinc finger and BTB domain containing 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000696">
        <property type="entry name" value="Zinc finger and BTB domain containing 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000917">
        <property type="entry name" value="Zinc finger and BTB domain containing 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001640">
        <property type="entry name" value="Zinc finger and BTB domain containing 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001792">
        <property type="entry name" value="Zinc finger and BTB domain-containing 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003097">
        <property type="entry name" value="Zinc finger and BTB domain-containing 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000909">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001061">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001143">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050888">
        <property type="entry name" value="ZnF_C2H2-type_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24406">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR CTCFL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="358"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="429"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="486"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="515"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="543"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="572"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="600"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="628"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="668"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="697"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="757"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="858" mass="95658" checksum="2657710EB913D2FB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJ69</accession>
    <name>A0A452GJ69_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GJ69"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001657"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001882.1">
        <property type="protein sequence ID" value="ENSGAGP00000001657.1"/>
        <property type="gene ID" value="ENSGAGG00000001326.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15005">
        <property type="entry name" value="7tmA_SREB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051509">
        <property type="entry name" value="GPCR_Orphan/Phoenixin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19268">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19268:SF8">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 27-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="38"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="369" mass="41118" checksum="203692342AADFAA8" modified="2025-10-08" version="2">MAEAGGGGEEVGGSMPAWSASGLKLATLGLILCVSLAGNVLFAWLILKDRHLHRAPYYLLLDLCLADALRSLACFPFVMLSVRSGAAAWPHGPLSCKVLAFLAVLFCFHAAFLLFCVGVTRYMAIAHHRFYAKRMTGWTCLAMVCMVWTLAMAMAFPPVFDVGTYKFIREEEQCIFEHRYVKANDTLGFMLMLAAVIAATHLVYGKLLFFIHGHRKMKPAQLVPAISQNWTFHGPGATGQAAANWTAGFGRGPTPPTLVGIRQATHSQIKRLLVLEEFKMEKRICKMFYMITLLFLLLWSPYIVACYLRVFIKASTIPQAYLTASVWMTFAQAGVNPILCFVFNKEFRVCFRAHFPCSGGLTFSSDFKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKJ5</accession>
    <name>A0A452GKJ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GKJ5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002255"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002579.1">
        <property type="protein sequence ID" value="ENSGAGP00000002255.1"/>
        <property type="gene ID" value="ENSGAGG00000001832.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15231">
        <property type="entry name" value="7tmA_OR5V1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="316" mass="35258" checksum="3AA2C863077BA292" modified="2019-05-08" version="1">MENQTQVTEFIFLGFASLLHGQAFLFLVFLVIYLVTLVGNSMIFTLIQLDSHLHSPMYFLLSHLSCLDICFSSVTVPKILVNFLREQETISYWECMAQMFFLMSCAGAECALLAVMAYDRYAAICNPLRYTDIMSRRVCIPLAMGSWLWGLLDSAIHTFMASSLSFCGANQLHHIFCDVPPLLKIACSDTYVNEMGLHAASIFVGLGPFLLIVISYLYILAAILKIRSNTGRHKAFSTCASHLIVVTIYFGMANLNYNRPSAGYSMGVDTLVSTLYCIVTPMLNPLIYSLRNQEVKGALRKALGSQRRECASPGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GL51</accession>
    <name>A0A452GL51_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methanethiol oxidase</fullName>
            <shortName evidence="3">MTO</shortName>
            <ecNumber evidence="3">1.8.3.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Selenium-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the oxidation of methanethiol, an organosulfur compound known to be produced in substantial amounts by gut bacteria. Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 3">
            <text>methanethiol + O2 + H2O = hydrogen sulfide + formaldehyde + H2O2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16007"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="EC" id="1.8.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Organosulfur degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="3">May associate with Golgi membrane. May associate with the membrane of autophagosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the selenium-binding protein family.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.4" evidence="3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002393"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002744.1">
        <property type="protein sequence ID" value="ENSGAGP00000002393.1"/>
        <property type="gene ID" value="ENSGAGG00000001587.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018549">
        <property type="term" value="F:methanethiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008430">
        <property type="term" value="F:selenium binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008826">
        <property type="entry name" value="Se-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300:SF0">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05694">
        <property type="entry name" value="SBP56"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0711">Selenium</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="483" mass="53894" checksum="89D726AAB6E0B48A" modified="2019-05-08" version="1">MLPLGKSNPVRESFKACFGDSAELICVDLNKLSALQGPREEIVYLPCIYRNTGIEKPDYLATVDVDPKSSHYCQVIHRLPMPNLRDELHHSGWNTCSSSFGDVTKKRNRLLLPSLISSRIYVVDVGTDMRAPRIHKVCLRRLQEMSHXRLPMPNLRDELHHSGWNTCSSSFGDVTKKRNRLLLPSLISSRIYVVDVGTDMRAPRIHKMIEPVDVFWKCGLANPHTTHCLGNGEIMISSMGDPSGNGKGSYGRHINVWDWTTHVFTQSIDLGEDSIPLEIRFLHNPDAAEGYVGCALSSAIHRFYKTGNGTWAAEKVVQVPSKKVNGWLFPDMPGVPGRQDSKRWANRCGGRPRAEVSARAFCDQGEEGARWTTDDPAELGWQEIICLHFPLQCMGQAILPRPCQVRNLSWMGNSRASHRQLCLQQKSIFMSPAAEVGGFCTTSKGPWMCAGGLALLAHCTLLTHISVSHTSENQPTLNRHNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMH5</accession>
    <name>A0A452GMH5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Surfactant protein C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pulmonary surfactant-associated protein C</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Surface film</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMH5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003048"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003498.1">
        <property type="protein sequence ID" value="ENSGAGP00000003048.1"/>
        <property type="gene ID" value="ENSGAGG00000002444.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000003501.1">
        <property type="protein sequence ID" value="ENSGAGP00000003050.1"/>
        <property type="gene ID" value="ENSGAGG00000002444.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007585">
        <property type="term" value="P:respiratory gaseous exchange by respiratory system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007084">
        <property type="entry name" value="BRICHOS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001729">
        <property type="entry name" value="SP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015091">
        <property type="entry name" value="Surfactant_protein_propep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10800">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10800:SF4">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04089">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08999">
        <property type="entry name" value="SP_C-Propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01039">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00019">
        <property type="entry name" value="SF_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50869">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0305">Gaseous exchange</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0767">Surface film</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="BRICHOS" evidence="12">
        <location>
            <begin position="157"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="255" mass="28710" checksum="01CFB99E3360DB68" modified="2019-05-08" version="1">MSKWHRRSSKMEISIKEALIEDPLHYNALDSEMDICPSCTDCCPSCPSCTNCCPGCPSCKSCCSCWKRVFCCLPKLLCHLPKFPCCPLHIKRILIIVVVIVLIVVVVLGALLLGLHVTQEHTETVLRMTIEGLEGEESRLRLSMDWEEEVATFHIDAGTHDPGTVVYDFSNLLISYKSWQGRACYITKMDKENIQGLDTVVKAFHQHLQLKSLIPTPEQGEEEEKEKGFPMPLADRSILGATVNILCSNIPIYWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GN69</accession>
    <name>A0A452GN69_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GN69"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003294"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003795.1">
        <property type="protein sequence ID" value="ENSGAGP00000003294.1"/>
        <property type="gene ID" value="ENSGAGG00000002663.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000094">
        <property type="entry name" value="Homeobox protein Hox-A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025281">
        <property type="entry name" value="DUF4074"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664:SF5">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664">
        <property type="entry name" value="PROTEIN ZERKNUELLT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13293">
        <property type="entry name" value="DUF4074"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="178"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="180"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="61"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="383"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="394"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="415" mass="44886" checksum="744F8FC11918BC75" modified="2019-05-08" version="1">MLFEQGPQALAIPESKMQKTAYYDNSGLFGGYTYSKADVYSYSSAHQPYAQAALDTDYPSSACSIQSSAPIRAPTHKSSELNGSCMRTNGGSQAGSQPPGIGEQQQPPALPPSSPPNPTNAAPAKKNKGGPNSSSSSTATISKQIFPWMKESRQNSKQKNTCAASAGDNCEDKSPPGPASKRVRTAYTSAQLVELEKEFHFNRYLCRPRRVEMANLLNLTERQIKIWFQNRRMKYKKDQKAKGIMHSPVGQSPDRSPPLSGPNHVGYSSQLPTVNSLSYDAPSPTSFAKSQQNMYGLAAYTAPLSSCLPQQKRYPGAEYDHHTMQSSGGFANPNLQGSPVYVGGNFVDSMPAPGPMFNIGHLSHPSSASVDYSCAAQIPGNHHHGPCDPHPTYTDLTSHHTSQGRIQEAPKLTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNA0</accession>
    <name>A0A452GNA0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GNA0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003348"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003858.1">
        <property type="protein sequence ID" value="ENSGAGP00000003348.1"/>
        <property type="gene ID" value="ENSGAGG00000002706.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042805">
        <property type="term" value="F:actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060537">
        <property type="term" value="P:muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09481">
        <property type="entry name" value="LIM1_CRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09482">
        <property type="entry name" value="LIM2_CRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000001">
        <property type="entry name" value="Cysteine and glycine-rich protein 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215:SF1">
        <property type="entry name" value="CYSTEINE AND GLYCINE-RICH PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="118"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="193" mass="20789" checksum="4BB514555B496DE6" modified="2019-05-08" version="1">MPNWGGGAKCGACDKSVYHAEEIQCNGRSFHKTCFHCMACRKALDSTTVAAHESEIYCKTCYGRKYGPKGIGFGQGAGCLSTDTGEHLGLDLQHSPKPARPSTPTNPSKFTKKLGDVDKCPRCGKSVYAAEKIMGGGKPWHKTCFRCAICGKSLESTNVTDKDGEIYCKVCYAKNFGPKGIGFGGLTQVEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GP58</accession>
    <name>A0A452GP58_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dynein assembly factor with WD repeat domains 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Required for axonemal dynein assembly and ciliary motility in ciliated organs, including Kupffer's vesicle, during embryogenesis. Facilitates the onset of robust cilia motility during development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with IFT46.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
            <location evidence="9">Cytoskeleton</location>
            <location evidence="9">Flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the WD repeat WDR69 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GP58"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003694"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004273.1">
        <property type="protein sequence ID" value="ENSGAGP00000003694.1"/>
        <property type="gene ID" value="ENSGAGG00000001916.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000227">
        <property type="entry name" value="Dynein assembly factor with WDR repeat domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000250">
        <property type="entry name" value="Dynein assembly factor with WDR repeat domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001051">
        <property type="entry name" value="dynein assembly factor with WDR repeat domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846:SF0">
        <property type="entry name" value="PRE-MRNA PROCESSING FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="110"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="152"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="195"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="237"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="279"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="321"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="363"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="405"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="437" mass="48409" checksum="A0AD5F49E8CF7B7C" modified="2019-05-08" version="1">DGRRRKQMGEGVRNEWVAEEAPRTLAGLIVYCFFKGIILEYVQGGELKTKSIDLLDLSPGTDVGALVEEIRKAEPLITASRKEQVIHLVLRLQEKLGQQEDHKFYLFKVLRAHILPLTNVAFNKSGSCFITGSYDRTCKIWDTASGEELRTLEGHRNVVYAIAFNNPYGDKIATGSFDKTCKLWSAETGKCYHTFRGHTAEIVCLSFNLQSTLIATGSMDTTAKLWDIQNGEEVVMLTGHSAEIIALSFNTTGDRIITGSFDHTVAVWDVGTGRKLHTLIGHRAEISSAQFNWDCSLIVTGSMDKTCMLWNAVTGTRVATLTGHDDEVLDVCFDYTGQRIATSSADGSARVYNAETKKCIAKMEGHGGEISKICFNPQGSRILTASSDKTARLWDPHTGQCIQILEGHTDEIFSCAFNYKGDIIITGSKDNTCRIWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQS3</accession>
    <name>A0A452GQS3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Sodium/potassium-transporting ATPase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">The catalytic subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Uses ATP as an energy source to pump H(+) ions to the gastric lumen while transporting K(+) ion from the lumen into the cell. Remarkably generates a million-fold proton gradient across the gastric parietal cell membrane, acidifying the gastric juice down to pH 1. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). The release of the H(+) ion in the stomach lumen is followed by binding of K(+) ion converting the pump conformation back to the E1 state.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>K(+)(out) + ATP + H2O + H(+)(in) = K(+)(in) + ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:22044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:22045"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">The gastric H(+)/K(+) ATPase pump is composed of the catalytic alpha subunit ATP4A and the regulatory beta subunit ATP4B. Interacts (via the P-domain) with ATP4B (via N-terminus); this interaction stabilizes the lumenal-open E2 conformation state and prevents the reverse reaction of the transport cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Cell membrane</location>
            <topology evidence="21">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQS3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004190"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004910.1">
        <property type="protein sequence ID" value="ENSGAGP00000004190.1"/>
        <property type="gene ID" value="ENSGAGG00000003136.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008900">
        <property type="term" value="F:P-type potassium:proton transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02608">
        <property type="entry name" value="P-type_ATPase_Na-K_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000079">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000003">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000001">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000004">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000095">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015127">
        <property type="entry name" value="ATPase_P-typ_H/K-transp_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005775">
        <property type="entry name" value="P-type_ATPase_IIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01106">
        <property type="entry name" value="ATPase-IIC_X-K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF10">
        <property type="entry name" value="POTASSIUM-TRANSPORTING ATPASE ALPHA CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09040">
        <property type="entry name" value="H-K_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="16 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 21" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 21" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 21" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="293"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="792"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="957"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="988"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="54"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU362084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1026" mass="114130" checksum="D2127A66BAE24DBB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GS50</accession>
    <name>A0A452GS50_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vacuolar protein sorting-associated protein VTA1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with VPS4B. Interacts with CHMP1B. Interacts with CHMP2A; the interaction probably involves the open conformation of (polymerized) CHMP2A. Interacts with CHMP3. Interacts with CHMP5; the interaction involves soluble CHMP5. Interacts with IST1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VTA1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GS50"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004788"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005606.1">
        <property type="protein sequence ID" value="ENSGAGP00000004788.1"/>
        <property type="gene ID" value="ENSGAGG00000003936.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.420:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein VTA1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.270:FF:000001">
        <property type="entry name" value="vacuolar protein sorting-associated protein VTA1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.270">
        <property type="entry name" value="Vacuolar protein sorting-associated protein vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044538">
        <property type="entry name" value="Vta1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039431">
        <property type="entry name" value="Vta1/CALS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023175">
        <property type="entry name" value="Vta1/CALS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041212">
        <property type="entry name" value="Vta1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04652">
        <property type="entry name" value="Vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18097">
        <property type="entry name" value="Vta1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vta1/callose synthase N-terminal" evidence="13">
        <location>
            <begin position="55"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Vta1 C-terminal" evidence="14">
        <location>
            <begin position="302"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="196"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="268"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="343" mass="37264" checksum="66E9BC8C13918A08" modified="2019-05-08" version="1">MRASAGGCPCTGSGSGPRGVGVVLVVVASAAAAGRVWGVMAAAALPPLPEQFKSLQHHLRTGQELDKREPVVAYYCRLYAMQTGMKLDSKTPECRKFLSKLMDQLETMKKQLGDNEAITQEIVGCAHVENYALKMFLYADNEDRAGRFHKNMIKSFYTASLLLDVLSVFGELTEENVQHRKYARWKAAYIHNCLKNGETPQPGPIGMEEEDDDAENEEGSSSALPTQVSQPSSSTYDPNMPTSNYSGIHIPPGAHAPANTPAEVPHSTGVTSNTIQPTPQNIPAVDPSLYNAQSAGEIRLTPEDFARAQKYCKYAGSALQYEDVSTAVQNLQKALKLLTTGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSC0</accession>
    <name>A0A452GSC0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein S100</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">S100 calcium-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy. Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9. Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis. Also modulates the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels. Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes. In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the S-100 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GSC0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004786"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005600.1">
        <property type="protein sequence ID" value="ENSGAGP00000004782.1"/>
        <property type="gene ID" value="ENSGAGG00000003931.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000005604.1">
        <property type="protein sequence ID" value="ENSGAGP00000004786.1"/>
        <property type="gene ID" value="ENSGAGG00000003931.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00213">
        <property type="entry name" value="S-100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000044">
        <property type="entry name" value="Protein S100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034325">
        <property type="entry name" value="S-100_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001751">
        <property type="entry name" value="S100/CaBP7/8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013787">
        <property type="entry name" value="S100_Ca-bd_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639:SF51">
        <property type="entry name" value="PROTEIN S100-A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639">
        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01023">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01394">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00303">
        <property type="entry name" value="S100_CABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="101" mass="11727" checksum="B3A4B95DF87CDE86" modified="2019-05-08" version="1">MAIPLEQALAVMVSTFHKYSARQGDKFKLSKAELKELLTKELPSFQSKQMDDAEFQKIMKDLDVNKDNEVDFQEFACFLACVAMGFNDFFKDHCQKQLHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUN7</accession>
    <name>A0A452GUN7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GUN7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005800"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006763.1">
        <property type="protein sequence ID" value="ENSGAGP00000005800.1"/>
        <property type="gene ID" value="ENSGAGG00000004719.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046329">
        <property type="term" value="P:negative regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18470">
        <property type="entry name" value="BACK_KLHL31_KBTBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18260">
        <property type="entry name" value="BTB_POZ_KLHL31_KBTBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030604">
        <property type="entry name" value="BTB_POZ_KLHL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF17">
        <property type="entry name" value="KELCH-LIKE PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="73"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="634" mass="70722" checksum="CB867E8B0D7F7827" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWB9</accession>
    <name>A0A452GWB9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GWB9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006377"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007418.1">
        <property type="protein sequence ID" value="ENSGAGP00000006377.1"/>
        <property type="gene ID" value="ENSGAGG00000005147.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029134">
        <property type="entry name" value="DUF4647"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36130">
        <property type="entry name" value="RIKEN CDNA 4933430I17 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36130:SF1">
        <property type="entry name" value="RIKEN CDNA 4933430I17 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15504">
        <property type="entry name" value="DUF4647"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="320" mass="36481" checksum="E86969BF633855B0" modified="2019-05-08" version="1">MVTADDSQWDETICDMAVCQHPQCWDTIRRIEQGLPRIRLRNFDSLCRASSSESKDELPTLKIINFPSNCSPRGRIRCSNCNRTISTFTALETSFSPSLYETSDYDFSDRNSEVSSERTHFPGLNSAVESHTIPQTEMNFTYLNRVGNLQVMDLSEVAALKQDYHPDSGNLIVKWIPNIRKKSQRPEVEAVTELKPVRRLGVKTLTSASLLSLKEWQRKGRDLDMKRKKSKKVPSGGQPYQLLTNRSQKINLVTKKIKSIPSHRGDRHLQTATIVELQDQGALQLGNWRVDVPDATEKHTRSLLVQKVPSLYKQINVMRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWX5</accession>
    <name>A0A452GWX5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Neuronal-specific septin-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GWX5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006605"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007680.1">
        <property type="protein sequence ID" value="ENSGAGP00000006605.1"/>
        <property type="gene ID" value="ENSGAGG00000005342.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000387">
        <property type="entry name" value="neuronal-specific septin-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008114">
        <property type="entry name" value="Septin3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01741">
        <property type="entry name" value="SEPTIN3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="7">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="293" mass="33826" checksum="A8960BFC02501A5E" modified="2019-05-08" version="1">MLHSAPLHLSGQSGLGKSTLVNTLFKSQVSRKSSGWNREEKIPKTVEIKAIGHVIEEGGVKMKLTVIDTPGFGDQINNENCWEPIEKYINEQYEKFLKEEVNIARKKRIPDTRVHCCLYFISPTGHSLRPLDLEFMKHLSKVVNIIPVIAKADTMTLEEKTEFKQRVRKELEVNGIEFYPQKEFDEDLEDKTENDKIRQESMPFAVVGSDKEYQVNGKRILGRKTPWGVIEVENLNHCEFALLRDFVIRTHLQDLKEVTHNIHYETYRAKRLNDNGGLPPVTTETEENHESNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GY55</accession>
    <name>A0A452GY55_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GY55"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007092"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008212.1">
        <property type="protein sequence ID" value="ENSGAGP00000007092.1"/>
        <property type="gene ID" value="ENSGAGG00000005702.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="49" mass="5324" checksum="1B1EA752455DC5E8" modified="2019-05-08" version="1">WSQTGVIKCKAAVAWEVGKPLSIEEVEVAPPKAHELSKTRCKNVFPSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYC8</accession>
    <name>A0A452GYC8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GYC8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007065"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008183.1">
        <property type="protein sequence ID" value="ENSGAGP00000007065.1"/>
        <property type="gene ID" value="ENSGAGG00000005676.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001594">
        <property type="entry name" value="Immunoglobulin heavy variable 9-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="15"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="139" mass="15518" checksum="4AFD42449C75684E" modified="2019-05-08" version="1">IDVVCETSVFCQLTPADVQSVALTQSGPALKKPGESHTLQCATSGFALSSAWMGWVRQEPGKGLERLAEYYDDKYYASSIQGRFTASKDTSNFYLHMTSLKGEDTAVYYCARDTVRGSESELRQKPSRQLQRKFSAWGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZ35</accession>
    <name>A0A452GZ35_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZ35"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007439"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008594.1">
        <property type="protein sequence ID" value="ENSGAGP00000007439.1"/>
        <property type="gene ID" value="ENSGAGG00000005967.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000008598.1">
        <property type="protein sequence ID" value="ENSGAGP00000007443.1"/>
        <property type="gene ID" value="ENSGAGG00000005967.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098797">
        <property type="term" value="C:plasma membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026501">
        <property type="entry name" value="Limbin/EVC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16795:SF13">
        <property type="entry name" value="EVC COMPLEX MEMBER EVC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16795">
        <property type="entry name" value="LIMBIN/ELLIS-VAN CREVELD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="99"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="259"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1104"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="880"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1002"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1105"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1115"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1125"/>
            <end position="1136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1136" mass="128689" checksum="79C6ABA45CC4DA2C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H203</accession>
    <name>A0A452H203_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pancreatic polypeptide</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the NPY family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H203"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008530"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009817.1">
        <property type="protein sequence ID" value="ENSGAGP00000008530.1"/>
        <property type="gene ID" value="ENSGAGG00000006775.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031841">
        <property type="term" value="F:neuropeptide Y receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007631">
        <property type="term" value="P:feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00126">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001955">
        <property type="entry name" value="Pancreatic_hormone-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020392">
        <property type="entry name" value="Pancreatic_hormone-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10533">
        <property type="entry name" value="NEUROPEPTIDE Y/PANCREATIC HORMONE/PEPTIDE YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10533:SF5">
        <property type="entry name" value="PRO-NEUROPEPTIDE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00159">
        <property type="entry name" value="Hormone_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00278">
        <property type="entry name" value="PANCHORMONE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00309">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00265">
        <property type="entry name" value="PANCREATIC_HORMONE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50276">
        <property type="entry name" value="PANCREATIC_HORMONE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0027">Amidation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019309330" description="Pancreatic polypeptide" evidence="7">
        <location>
            <begin position="24"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="8696" checksum="BFB0A2A5FE76B5A9" modified="2019-05-08" version="1">MRWASLFSLTCCVALLLGHLGSAAPMHPKYPGDNAPVEDLVQFYNELQQYLNMVTRPRYGKRSSSRTLCGDPYDPSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H235</accession>
    <name>A0A452H235_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flavodoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)H dehydrogenase (quinone) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H235"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008624"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009916.1">
        <property type="protein sequence ID" value="ENSGAGP00000008624.1"/>
        <property type="gene ID" value="ENSGAGG00000006835.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000054">
        <property type="entry name" value="NAD(P)H dehydrogenase, quinone 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003680">
        <property type="entry name" value="Flavodoxin_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051545">
        <property type="entry name" value="NAD(P)H_dehydrogenase_qn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10204">
        <property type="entry name" value="NAD P H OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10204:SF33">
        <property type="entry name" value="RIBOSYLDIHYDRONICOTINAMIDE DEHYDROGENASE [QUINONE]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02525">
        <property type="entry name" value="Flavodoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like fold" evidence="3">
        <location>
            <begin position="20"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="225" mass="25369" checksum="7B9D3DA9672D2045" modified="2019-05-08" version="1">MYPSSWYLGDSLFFLTPGRKVLVFYAHQEPKSLNGSLKNCAVEELSKQGCNIIMSDLYAMRFEPRATRNDIIGCLHNSEHVDYGVESWEAYKRRCLASDLIEEQRKVQEAKLVIFQFPLYLFSMPAIMKDWMDTVLVQGFAHNFPQCYDDDLLKVRSLDCYIRPGIGNLWPAAHQGKQIMALTGHGSLSQANGGSGKQRGSRDVLAAAPHCPHWPGAVNCGQWEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H297</accession>
    <name>A0A452H297_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LRRNT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H297"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008627"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009919.1">
        <property type="protein sequence ID" value="ENSGAGP00000008627.1"/>
        <property type="gene ID" value="ENSGAGG00000006839.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000146">
        <property type="entry name" value="podocan isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF20">
        <property type="entry name" value="PODOCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019451548" description="LRRNT domain-containing protein" evidence="6">
        <location>
            <begin position="24"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="7">
        <location>
            <begin position="49"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="568"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="577"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="589" mass="67448" checksum="F182D787C9020FF8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2G0</accession>
    <name>A0A452H2G0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H2G0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008768"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010075.1">
        <property type="protein sequence ID" value="ENSGAGP00000008768.1"/>
        <property type="gene ID" value="ENSGAGG00000006931.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="214" mass="23502" checksum="F886F57B5A1748D7" modified="2019-05-08" version="1">VVLPYIMSSTLRRLSVTSVTSSVVSTSSTSSDSTSSRPGSDGSILEPLLERRLSTASKAEELPVKEDADNRINKFKRKDWSISKSQVIVEKEPEAELTSKTRPKSLQLGDSRLTLLQASSLQQSTPLSPPPITPKTPRTHSFPSLQTDGLATANFQSTPPPPPPKSKPYENSNSRNSLEVGKLELTLFWKRALEQATPVELSQLLLGWCYLKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2Q4</accession>
    <name>A0A452H2Q4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WAS/WASL interacting protein family member 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000008801"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010117.1">
        <property type="protein sequence ID" value="ENSGAGP00000008801.1"/>
        <property type="gene ID" value="ENSGAGG00000006963.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019450694" description="WAS/WASL interacting protein family member 2" evidence="2">
        <location>
            <begin position="17"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16276" checksum="FD7DEDF29BE402FF" modified="2019-05-08" version="1">MAVLLCSFFHEHLGLAATRDWLLGWLGHRSYCSCVLICADHGFYCSPSTPAPXXXXXXXXXXXXXXXPPPTRTPAGPPPPPPPVRNGHRDSIATVRSFLDDFESKYSFHPVEDFPAPEEYKHFQRTYPSKTNRASRGAPPLPPIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H366</accession>
    <name>A0A452H366_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H366"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008975"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010309.1">
        <property type="protein sequence ID" value="ENSGAGP00000008975.1"/>
        <property type="gene ID" value="ENSGAGG00000007050.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000145">
        <property type="entry name" value="WD repeat domain phosphoinositide-interacting protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="386"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="387"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="437"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="447" mass="49380" checksum="74CCBE145E7C439B" modified="2019-05-08" version="1">MEAAAGAGPAEAAGPEALSCFAYNQDCTSLAIGTKTGYRLFSLSSVEQLDQVHANNEIPDVYIVERLFSSSLVVVVSHTKPRQMNVYHFKKGTEICNYSYSSNILSIRLNRQRLIVCLEESIYIHNIKDMKLLKTILDTPPNTTGLCALSINHSNSYLAYPGSSTIGEIVLYDGNNLRTVCTIPAHDGPLAAITFNSMGSKLASASEKGTVIRVFSVPDGQKLYEFRRGMKRYVNISSLVFSMDSQFLCASSNTETVHIFKLEHITDSRPEEPPTWSGYMGKMFMAATNYLPAQVSDMMNQDRAFATVRLNFSGQRNICTLSTIQKLPRLLVTTSDGHLYIYNLDPQDGGECVLIKKHNLLSSEKTDENKENDLRPPLPQSYAATVARQSTMPSSSSMPGYSEDGGALRGEVIPEHEFATGPVRLDDENEFPPVSIRRMRNQRGKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3D7</accession>
    <name>A0A452H3D7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase M12B propeptide domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H3D7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008932"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010263.1">
        <property type="protein sequence ID" value="ENSGAGP00000008932.1"/>
        <property type="gene ID" value="ENSGAGG00000007063.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF256">
        <property type="entry name" value="PEPTIDASE M12B DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M12B propeptide" evidence="2">
        <location>
            <begin position="23"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="211" mass="24125" checksum="579302D07018CFC3" modified="2019-05-08" version="1">KAKISPLLFFPEHFIRTLQEYHVVGPAKVDASGHFLSYSLHHHTSDARRKRDFSRKENLVYYKINYKEKELFFNLTVHRGFLSANYVLERRYGNHTGAKIVPYTGTSCHLIGTVLQPNSGTGPAAISTCNGLTGYFRLPQGDYFIEPVKKHPLEEGIPYPHIIYRANILQNALRQRRETTTDKKQACGLNGMNFVGDFCLLVAEFFLTQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3E4</accession>
    <name>A0A452H3E4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H3E4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009150"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010509.1">
        <property type="protein sequence ID" value="ENSGAGP00000009150.1"/>
        <property type="gene ID" value="ENSGAGG00000007232.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000019">
        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000056">
        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF7">
        <property type="entry name" value="SIMILAR TO MYOSIN, LIGHT POLYPEPTIDE 6, ALKALI, SMOOTH MUSCLE AND NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="1" id="KW-0518">Myosin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="151" mass="16931" checksum="5E61613E369B7369" modified="2019-05-08" version="1">MCDFSEDQTAEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSDEMNMKTLSFEQFLPMMQTIAKNKDQGCFEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEVEILVAGHEDSNGCINYEAFVRHILSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3T5</accession>
    <name>A0A452H3T5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3T5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009105"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010447.1">
        <property type="protein sequence ID" value="ENSGAGP00000009105.1"/>
        <property type="gene ID" value="ENSGAGG00000007182.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020066">
        <property type="entry name" value="Cortexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16736:SF3">
        <property type="entry name" value="CORTEXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16736">
        <property type="entry name" value="CORTEXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11057">
        <property type="entry name" value="Cortexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="82" mass="9122" checksum="4F114D35D8EFD1FE" modified="2019-05-08" version="1">WGGGISLQVSVTPLGEPPATGLGMDAEQKTVFAFVIFLLVFLVMLMVRCFRILLDPYSRMPASSWTDHKEGLERGQFDYALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3U9</accession>
    <name>A0A452H3U9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Arachidonate lipoxygenase 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="9">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3U9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009258"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010626.1">
        <property type="protein sequence ID" value="ENSGAGP00000009258.1"/>
        <property type="gene ID" value="ENSGAGG00000007252.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01753">
        <property type="entry name" value="PLAT_LOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000002">
        <property type="entry name" value="Arachidonate 5-lipoxygenase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001885">
        <property type="entry name" value="LipOase_mml"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042062">
        <property type="entry name" value="PLAT_LOX_verte"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00467">
        <property type="entry name" value="MAMLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="14">
        <location>
            <begin position="2"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="15">
        <location>
            <begin position="98"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="644"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="542"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Essential for stabilizing binding to COTL1" evidence="11">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="672" mass="75020" checksum="7CCA568C043C7A58" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H5R8</accession>
    <name>A0A452H5R8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H5R8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010005"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011487.1">
        <property type="protein sequence ID" value="ENSGAGP00000010005.1"/>
        <property type="gene ID" value="ENSGAGG00000007858.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="7">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="57"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="231" mass="25387" checksum="4C2132FB9CA2371C" modified="2019-05-08" version="1">MQLEHCLSPSIMLSKKFLNVSSSYPHAGGSELALHDHPIISTTDNLERSSPLKKITRGMTNQSDTDNFPDSKDTPGDVQRNKLSPVLDGVSELRHSFDGSAADRYLLSQSSQPQSAAAAPSTMFPYPSQHGPAHPAFSIASPSRYMAHHPVITNGAYNSLLSNSSPQGYPTAGYPYPQQYGHSYQGAPFYQFSSTQPGLVPGKAQVYLCNRPLWLKFHRHQTEMIITKQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H5W3</accession>
    <name>A0A452H5W3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein SYS1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYS1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H5W3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010038"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011521.1">
        <property type="protein sequence ID" value="ENSGAGP00000010038.1"/>
        <property type="gene ID" value="ENSGAGG00000007879.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019185">
        <property type="entry name" value="Integral_membrane_SYS1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952:SF0">
        <property type="entry name" value="PROTEIN SYS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09801">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="16974" checksum="760E7E7E0421929E" modified="2019-05-08" version="1">MAGQFRSYVWDPVLILTQIVLMQAVYYSSLGLWLALVDSLVQNSPSLDQIFNYEILGFSTPPGRLSMIAFILNLLFIHTGIGHLLSGISIRRHFYGNWLRCWCRSRRALRTLWSALLSRGGPGCEVAVTVSSRVSQSAARGGGGGLCPGGVGSGIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7X3</accession>
    <name>A0A452H7X3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear receptor subfamily 0 group B member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nuclear receptor DAX-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Nuclear receptor that lacks a DNA-binding domain and acts as a corepressor that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer. Interacts with NR5A1, NR5A2, NR0B2 and with COPS2. Interacts with ESRRB; represses ESRRB activity at the GATA6 promoter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nuclear hormone receptor family. NR0 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H7X3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010818"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012395.1">
        <property type="protein sequence ID" value="ENSGAGP00000010818.1"/>
        <property type="gene ID" value="ENSGAGG00000008416.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030325">
        <property type="term" value="P:adrenal gland development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008584">
        <property type="term" value="P:male gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07350">
        <property type="entry name" value="NR_LBD_Dax1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000027">
        <property type="entry name" value="nuclear receptor subfamily 0 group B member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033544">
        <property type="entry name" value="NR0B1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025900">
        <property type="entry name" value="Nuclear_receptor_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24081">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 0 GROUP B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24081:SF1">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 0 GROUP B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14046">
        <property type="entry name" value="NR_Repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NR LBD" evidence="16">
        <location>
            <begin position="15"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="267" mass="29761" checksum="E7D4299D1D2B1A47" modified="2019-05-08" version="1">MACVAGCRCCTERRRQSSILYNILKSEERPEAAQQPPPQEPPGPGCSCGSRRRVALRSPQVAGKAASAVLVKTLRFVKSVPCFQELPLDEQLVLVRSCWAPLLVLGLAQERVHFETVETAEPSMLQRILTDRRQEQQGQQHPDSSSPQLPSAGEIQTIKGFLAKCWSLDISTKEYAYLKGTVLFNPDLPGLQCVQYIQGLQREAQQALNEHVRLIHRGDQARFAKLNVVLSLLRSINANVIAELFFKPIIGTVNMDDMLLEMLCAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8C2</accession>
    <name>A0A452H8C2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Vesicle-associated membrane protein 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Synaptobrevin-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="22">Cytoplasmic vesicle</location>
            <location evidence="22">Phagosome membrane</location>
            <topology evidence="22">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Cytoplasmic vesicle</location>
            <location evidence="19">Secretory vesicle membrane</location>
            <topology evidence="19">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Golgi apparatus</location>
            <location evidence="18">trans-Golgi network membrane</location>
            <topology evidence="18">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Late endosome membrane</location>
            <topology evidence="20">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Lysosome membrane</location>
            <topology evidence="21">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Synapse</location>
            <location evidence="17">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H8C2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010969"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012563.1">
        <property type="protein sequence ID" value="ENSGAGP00000010969.1"/>
        <property type="gene ID" value="ENSGAGG00000008504.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15871">
        <property type="entry name" value="R-SNARE_VAMP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000004">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000006">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF179">
        <property type="entry name" value="VESICLE ASSOCIATED MEMBRANE PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 25" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="27" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <keyword evidence="4" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="5 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019233881" description="Vesicle-associated membrane protein 7" evidence="27">
        <location>
            <begin position="17"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="28">
        <location>
            <begin position="7"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="29">
        <location>
            <begin position="125"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00037845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00037863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00037875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00039269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00042194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="220" mass="24733" checksum="01BF0A11EBE25448" modified="2019-05-08" version="1">MAILFVVVARGTTILAKHAWCGGNFLEVTEQILAKIPSENNKLTYSHGNYLFHYICQDRIIYLCITDDNFERSRAFSFLNEIKKRFQTTYGSRAQTALPYAMNSEFSSILAAQLKHHSENKGTDQMVETQAQVDELKGIMVRNIDLVAQRGEKLELLIDKTENLVDSSVTFKTTSRNLARAMCMKNLKLTIIITVVSIVVIYVIVSAACGGLTWPSCVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8F1</accession>
    <name>A0A452H8F1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H8F1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011005"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012602.1">
        <property type="protein sequence ID" value="ENSGAGP00000011005.1"/>
        <property type="gene ID" value="ENSGAGG00000008522.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF208">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN TYPE 10 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="Ion transport" evidence="12">
        <location>
            <begin position="143"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="204" mass="23771" checksum="8A7022102734D92B" modified="2019-05-08" version="1">MADFSLPCSNNFCKFTRESLAAIEKRIAAKKECIGQKPEEKPRPQLDLKAFQKLPALYGNPPQELIGEPLEDLDPYYSDHKTFMVLNKGKTIFRFTATRALCILSPFHSIRRAAIKILVHKYPFSWLYSFYFFNNYTRIMGVILCLKSKSLDSPGWSKYVEYTFTGIYTFEVLIKILARGFCLNEFTFLRDLWNWLDFSVIVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9C7</accession>
    <name>A0A452H9C7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H9C7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011188"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012813.1">
        <property type="protein sequence ID" value="ENSGAGP00000011188.1"/>
        <property type="gene ID" value="ENSGAGG00000008657.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001946">
        <property type="term" value="P:lymphangiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001570">
        <property type="term" value="P:vasculogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22048">
        <property type="entry name" value="HMG-box_SoxF_SOX18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000008">
        <property type="entry name" value="transcription factor SOX-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033392">
        <property type="entry name" value="Sox7/17/18_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021934">
        <property type="entry name" value="Sox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF204">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12067">
        <property type="entry name" value="Sox17_18_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51516">
        <property type="entry name" value="SOX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="7">
        <location>
            <begin position="103"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Sox C-terminal" evidence="8">
        <location>
            <begin position="300"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="5">
        <location>
            <begin position="103"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="199"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="199"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="420" mass="46639" checksum="E6C6DDB74D4238BC" modified="2019-05-08" version="1">MNRPEPSYCREEISQPRGGRSWGPTAAPAAEPGLAYQPTPGADSASSRTPSPEPGFGFRPGAAGGEPGALGSAPSRSPSPDPGYGYSPTSGRAEGKPGEDSRIRRPMNAFMVWAKDERKRLAQQNPDLHNAVLSKMLGQSWKALSAGDKRPFVEEAERLRIQHLQDHPNYKYRPRRKKQAKKIKRMEPNILLHNLSQPCSDSFSMNHHSGSQPGHHQPPPLNHFRELHSMGSDIENYGLPTPEMSPLDVLEQTEPAFFPPHMQDDCSMIPFRGYHPHHQMEFPPEKPMGRDMALPYAQTPSHLADTMRTPHPSSMYYNQMCSGPQNILSAHLGQLSPPPEAHHVDSVDHLNQTELWTDVDRNEFDQYLNMSRTRPETSGLPYHVSLSKVTPRSISCEESSLISALSDASSAVYYSSCITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9P2</accession>
    <name>A0A452H9P2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H9P2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011486"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013149.1">
        <property type="protein sequence ID" value="ENSGAGP00000011486.1"/>
        <property type="gene ID" value="ENSGAGG00000008860.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12138">
        <property type="entry name" value="PRIMATE-EXPANDED PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12138:SF75">
        <property type="entry name" value="SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="61" mass="6690" checksum="E4A7C22607B330E2" modified="2019-05-08" version="1">IYLLIYLFSRQTLALLPRLECSGVISAHCKLRLPGSRHSPASASQVAEAGESLEPGKRRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HB29</accession>
    <name>A0A452HB29_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mineralocorticoid receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Nuclear receptor subfamily 3 group C member 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HB29"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011950"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013688.1">
        <property type="protein sequence ID" value="ENSGAGP00000011950.1"/>
        <property type="gene ID" value="ENSGAGG00000009209.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005496">
        <property type="term" value="F:steroid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07172">
        <property type="entry name" value="NR_DBD_GR_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07075">
        <property type="entry name" value="NR_LBD_MR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000004">
        <property type="entry name" value="Androgen receptor variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000029">
        <property type="entry name" value="mineralocorticoid receptor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050200">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48092">
        <property type="entry name" value="KNIRPS-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0754">Steroid-binding</keyword>
    <keyword evidence="11 17" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 17" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="19">
        <location>
            <begin position="23"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="20">
        <location>
            <begin position="118"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="369" mass="42258" checksum="A11A572EADEDAB35" modified="2019-05-08" version="1">GGLWVHKESTVKYIVGICFRIPSKVCLVCGDEASGCHYGVVTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKNCPACRLQKCLQAGMNLGARKSKKLGKLKGMHEEQQQQQQQQPPPQTLALSPSMVLESIEPEIVYAGYDNSKPDTAEHLLSTLNRLAGKQMVQVVKWAKILPGFRNLPLEDQITLIQYSWMCLSSFALSWRSYKHTNSQFLYFAPDLVFNEERMRQSAMFELCQGMHQISLQFVRLQISFEEYTIMKVLLLLSTVPKDGLKSQAAFEEMRANYIKELRKMVTKCPSNSGQSWQRFYQLTKLLDSIDLLEFCFYTFRESQALKVEFPAMLVEIISDQLPKVESGNAKALYFHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBV3</accession>
    <name>A0A452HBV3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ring finger protein 220</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HBV3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012242"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014022.1">
        <property type="protein sequence ID" value="ENSGAGP00000012242.1"/>
        <property type="gene ID" value="ENSGAGG00000009421.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052443">
        <property type="entry name" value="E3_ubiq-ligase_RNF220-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13459:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13459">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF220 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="24700" checksum="2C8A7ED783EC3195" modified="2019-05-08" version="1">MDLHRAAFKMENSSYLPNPLASPALMVLASTAEASRDASIPCQQPRPFGVPVSVDKDVHIPFTNGSYTFASMYHRQGGVPGAFANRDFPPSLLHLHPQFAPPNLDCTPISMLNHSGVGAFRPFASTEDRESYQSAFTPAKRLKNCHDTDSPHLRFSDADGKEYEFGTQLPSSSPGSLKLDDTGKKIFAVSGLISDRETSSSPEDRNDRCKYLRYPSQLYFAVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCA2</accession>
    <name>A0A452HCA2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Receptor for retinol uptake STRA6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HCA2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012404"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014196.1">
        <property type="protein sequence ID" value="ENSGAGP00000012404.1"/>
        <property type="gene ID" value="ENSGAGG00000009521.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016918">
        <property type="term" value="F:retinal binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019841">
        <property type="term" value="F:retinol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034632">
        <property type="term" value="F:retinol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071939">
        <property type="term" value="P:vitamin A import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026612">
        <property type="entry name" value="STRA6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21444">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21444:SF16">
        <property type="entry name" value="RECEPTOR FOR RETINOL UPTAKE STRA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14752">
        <property type="entry name" value="RBP_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0683">Retinol-binding</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0845">Vitamin A</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="430"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="475"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="516"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="666" mass="75209" checksum="4551BC2D75FC91BE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGU7</accession>
    <name>A0A452HGU7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Serine/threonine-protein kinase NIM1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">NIM1 serine/threonine-protein kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452HGU7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014097"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016139.1">
        <property type="protein sequence ID" value="ENSGAGP00000014097.1"/>
        <property type="gene ID" value="ENSGAGG00000010738.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14075">
        <property type="entry name" value="STKc_NIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000346">
        <property type="entry name" value="Serine/threonine-protein kinase NIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049571">
        <property type="entry name" value="NIM1K_STKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF49">
        <property type="entry name" value="NIM1 SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="74"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="419"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="436" mass="49661" checksum="BE5FBF12497CA7B0" modified="2019-05-08" version="1">MTTSYMNGGGLGNHRYARWNREDSGEDYGRAECYGEDEEGRKRRLTPFEKLTQDMSQDEKVVKELTLGKRIGFYRVRGEIGSGNFSQVKLGIHSLTKEKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNIIRLYEVVETLSKLHLVMEYAGGGELFIKISTEGKLAEMESKLIFSQIASAVKHMHDNNIIHRDLKAENVFYTSNTCVKVGDFGFSTISKKEETLNTFCGSPPYAAPELFRDENYVGAYVDIWALGILLYFMTTGTMPFRADTVAKLKKCILDGTYSLPPYLSDTCQKLIRGVLQPVPTARYSIECIMKNEWMQGIQHPKALDPFKLDPKYLAETSTLKEEEIEVKNTLQDLGITEEHIQNNRGRDSRSSITGIYRIVLHRVQKKRALETVPIVTHPEPKEQDLKKGHSSHRGIRHTSKLCSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HI13</accession>
    <name>A0A452HI13_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Katanin p80 subunit C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HI13"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014496"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016583.1">
        <property type="protein sequence ID" value="ENSGAGP00000014496.1"/>
        <property type="gene ID" value="ENSGAGG00000011018.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028021">
        <property type="entry name" value="Katanin_C-terminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042404">
        <property type="entry name" value="KATNBL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14682">
        <property type="entry name" value="KATNB1-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14682:SF1">
        <property type="entry name" value="KATNB1-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13925">
        <property type="entry name" value="Katanin_con80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Katanin p80 subunit C-terminal" evidence="5">
        <location>
            <begin position="147"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="302" mass="34274" checksum="413F41B66845E841" modified="2019-05-08" version="1">MASEAHNVRKWNLLHTKGCHIDLGRRISHSTNKNMKEGKKSPKQLASYTNRTTVGQTVTSSPFLFKVVYCRKKVHCYTSKPCYRKKQFPKVRGRDMANKENELACAGNLPAKVHDSHMLLLNTSDSGSSQTQGPSSKYSGFFSEVSQDHDTMAQVLFSRNLRLNVAFTFWRRRSISELIAYLVRIQDLGVVVDCLPVLTSSLQEEKPYISVGCCVDLFPLVKSLLKSKYEEYVIVGLNWLLAVIKRWWSELSAHIEKVKDGNIQILKQQLSGLWEQENHLTLVPGYTGNIAKDVGAYLLQLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HI40</accession>
    <name>A0A452HI40_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HI40"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014560"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016655.1">
        <property type="protein sequence ID" value="ENSGAGP00000014560.1"/>
        <property type="gene ID" value="ENSGAGG00000011060.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15962">
        <property type="entry name" value="7tmA_GPR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001151">
        <property type="entry name" value="GPR6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000723">
        <property type="entry name" value="GPR_3/6/12_orphan"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00649">
        <property type="entry name" value="GPR6ORPHANR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00644">
        <property type="entry name" value="GPRORPHANR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="78"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="351" mass="38342" checksum="A82DD44EB48C4E67" modified="2025-10-08" version="2">MNANKTQVNVEDVISSAAHRASAEMNESGSLHGTGVPLARVQASDGNSSLELSSQLPVFAINPWDVVLCISGTIIACENAIVVAIIFYTPTLRTPMFVLIGSLATADLLAGVGLILNFVVQYVIQSETISLITVGFLVASFAASVSSLLAITVDRYLSLYNALTYYSEKTVLCIHMMLIVTWGVSLCLGLLPVLGWNCLDDYSSCSIVRPLTKSNVTLLSASFFLVFIIMLHLYIKICKIVCRHAHQIALQQHFLTASHYVATKKGVSTLAIIIGTFGASWLPFAIYCVVGDLDYPSVYTYATLLPATYNSMINPIIYAYRNQEIQRAMWVLFCGCFQSKVSFRSRSPSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIG1</accession>
    <name>A0A452HIG1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Multiple EGF like domains 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000014683"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016790.1">
        <property type="protein sequence ID" value="ENSGAGP00000014683.1"/>
        <property type="gene ID" value="ENSGAGG00000011146.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000005">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000017">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000002">
        <property type="entry name" value="Multiple epidermal growth factor-like domains 10"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:001129">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000037">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000041">
        <property type="entry name" value="Tyrosine protein kinase receptor tie-1, putative"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011489">
        <property type="entry name" value="EMI_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042635">
        <property type="entry name" value="MEGF10/SREC1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24043:SF8">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24043">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51041">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EMI" evidence="8">
        <location>
            <begin position="20"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="100"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="261"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="460"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="764"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="846"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="1059"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="1148"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="1228"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="485"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="789"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="871"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="1084"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="1173"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="1248"/>
            <end position="1257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1352" mass="145747" checksum="2C5E83F1C5E51929" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HL58</accession>
    <name>A0A452HL58_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HL58"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015663"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017891.1">
        <property type="protein sequence ID" value="ENSGAGP00000015663.1"/>
        <property type="gene ID" value="ENSGAGG00000011818.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000017892.1">
        <property type="protein sequence ID" value="ENSGAGP00000015664.1"/>
        <property type="gene ID" value="ENSGAGG00000011818.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000017893.1">
        <property type="protein sequence ID" value="ENSGAGP00000015665.1"/>
        <property type="gene ID" value="ENSGAGG00000011818.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056524">
        <property type="entry name" value="KIF6/9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF67">
        <property type="entry name" value="KINESIN MOTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23735">
        <property type="entry name" value="KIF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="593"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="610"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="663" mass="74634" checksum="615BC99F06186E5A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HM29</accession>
    <name>A0A452HM29_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ferlin family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000015999"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018279.1">
        <property type="protein sequence ID" value="ENSGAGP00000015999.1"/>
        <property type="gene ID" value="ENSGAGG00000011993.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030315">
        <property type="term" value="C:T-tubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002281">
        <property type="term" value="P:macrophage activation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002280">
        <property type="term" value="P:monocyte activation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050765">
        <property type="term" value="P:negative regulation of phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001778">
        <property type="term" value="P:plasma membrane repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033292">
        <property type="term" value="P:T-tubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08373">
        <property type="entry name" value="C2A_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04011">
        <property type="entry name" value="C2B_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04018">
        <property type="entry name" value="C2C_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04017">
        <property type="entry name" value="C2D_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04037">
        <property type="entry name" value="C2E_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08374">
        <property type="entry name" value="C2F_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000009">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000021">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000026">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000033">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000037">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037726">
        <property type="entry name" value="C2A_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037720">
        <property type="entry name" value="C2B_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037722">
        <property type="entry name" value="C2C_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037723">
        <property type="entry name" value="C2D_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037724">
        <property type="entry name" value="C2E_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037725">
        <property type="entry name" value="C2F_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012968">
        <property type="entry name" value="FerIin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037721">
        <property type="entry name" value="Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012560">
        <property type="entry name" value="Ferlin_A-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012561">
        <property type="entry name" value="Ferlin_B-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032362">
        <property type="entry name" value="Ferlin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055072">
        <property type="entry name" value="Ferlin_DSRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546:SF44">
        <property type="entry name" value="DYSFERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546">
        <property type="entry name" value="FER-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22901">
        <property type="entry name" value="dsrm_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08165">
        <property type="entry name" value="FerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08150">
        <property type="entry name" value="FerB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08151">
        <property type="entry name" value="FerI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16165">
        <property type="entry name" value="Ferlin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00693">
        <property type="entry name" value="DysFN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01200">
        <property type="entry name" value="FerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01201">
        <property type="entry name" value="FerB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01202">
        <property type="entry name" value="FerI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2064"/>
            <end position="2086"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="199"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="356"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1132"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1305"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1580"/>
            <end position="1698"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1814"/>
            <end position="1962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="129"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2015"/>
            <end position="2036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="174"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="190"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="2017"/>
            <end position="2036"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2099" mass="239637" checksum="F13300219111B3BB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HM43</accession>
    <name>A0A452HM43_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HM43"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016057"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018341.1">
        <property type="protein sequence ID" value="ENSGAGP00000016057.1"/>
        <property type="gene ID" value="ENSGAGG00000012067.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="11002" checksum="FE87548AE19E9280" modified="2019-05-08" version="1">LESLPLLPTKADSLRVLHYNYTGKFILREKPTDLIDLTNVAFLIICTFIALDNWIVLLALSKNNRFHNHVLFISSLTRCDLLTGLVYKQMTKPAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNZ2</accession>
    <name>A0A452HNZ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Keratin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the avian keratin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HNZ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016739"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019110.1">
        <property type="protein sequence ID" value="ENSGAGP00000016739.1"/>
        <property type="gene ID" value="ENSGAGG00000012518.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003461">
        <property type="entry name" value="Keratin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31203">
        <property type="entry name" value="BETA-KERATIN-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31203:SF1">
        <property type="entry name" value="BETA-KERATIN-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02422">
        <property type="entry name" value="Keratin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0416">Keratin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="12306" checksum="866E1216DC50160C" modified="2019-05-08" version="1">MSCPLRKMTFSSLCYPECRVARPCPVTDSYNEPCVRQCPDSKVVIRPSPVVMTLPGPILRAPVVGPSSWDTFSFGGLNGSGGHYGGLSALEGYDGYGALCGLVSCHRYLSGNSVPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQX9</accession>
    <name>A0A452HQX9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Polymeric immunoglobulin receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. Binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Through its N-linked glycans ensures anchoring of secretory IgA (sIgA) molecules to mucus lining the epithelial surface to neutralize extracellular pathogens. On its own (free form) may act as a non-specific microbial scavenger to prevent pathogen interaction with epithelial cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts (mainly via CDR1-like domain) with dimeric IgA. Interacts (mainly via CDR2-like domain) with pentameric IgM.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQX9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017407"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019856.1">
        <property type="protein sequence ID" value="ENSGAGP00000017407.1"/>
        <property type="gene ID" value="ENSGAGG00000012946.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05716">
        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF82">
        <property type="entry name" value="POLYMERIC IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019009368" description="Polymeric immunoglobulin receptor" evidence="18">
        <location>
            <begin position="18"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="503"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="258"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="353"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="464"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="621" mass="68696" checksum="F1E4230A767FF44E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HR25</accession>
    <name>A0A452HR25_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
            <ecNumber evidence="9">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 17">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 17">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EC" id="3.1.3.48" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452HR25"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017470"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019925.1">
        <property type="protein sequence ID" value="ENSGAGP00000017470.1"/>
        <property type="gene ID" value="ENSGAGG00000012980.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14643">
        <property type="entry name" value="DSP_DUSP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000011">
        <property type="entry name" value="Dual specificity phosphatase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000011">
        <property type="entry name" value="Dual specificity phosphatase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008343">
        <property type="entry name" value="MKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF305">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000939">
        <property type="entry name" value="MAPK_Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01764">
        <property type="entry name" value="MAPKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="14">
        <location>
            <begin position="34"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="208"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="272"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="10">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="383" mass="42727" checksum="7CFE796D5520C7AC" modified="2019-05-08" version="1">MKNHLWGSSPRAPMAAAVPWKSVEWLQEELESGGGSSLLLLDCRPHELFESSHIETAINLAIPGLMLRRLKKGNLPIRSIIPNHEDKERFAKRCKADTVLLYDEATAEWQQDNGAPSSVLGLLLQKLRDDGCKAYYLKGGFNKFQTEYSEHCETNLDSSSPSNSPPASVLGLGGLRINSDCSDGESDREPSSATESDGSPIPNNQPAFPVQILPYLYLGCAKDSTNLDVLGKYGIKYILNVTPNLPNMFEHNGEFKYKQIPISDHWSQNLSQFFPEAIAFIDEARTKKCGILVHCLAGISRSVTVTVAYLMQKMNLSLNDAYDFVKRKKSNISPNFNFMGQLLDFERTLGLNSPCDNRSPNEQLYFTTPTNHNLFQLNTLEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSB3</accession>
    <name>A0A452HSB3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polyribonucleotide 5'-hydroxyl-kinase Clp1</fullName>
            <ecNumber evidence="7">2.7.1.78</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Polyadenylation factor Clp1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Polynucleotide kinase Clp1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Pre-mRNA cleavage complex II protein Clp1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CLP1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end dephospho-2'-deoxyribonucleoside-DNA + ATP = a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15669"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:13184"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:136416"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end dephospho-ribonucleoside-RNA + ATP = a 5'-end 5'-phospho-ribonucleoside-RNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54580"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:15179"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the tRNA splicing endonuclease complex. Component of pre-mRNA cleavage complex II (CF-II).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the Clp1 family. Clp1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.78" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452HSB3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017906"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020425.1">
        <property type="protein sequence ID" value="ENSGAGP00000017904.1"/>
        <property type="gene ID" value="ENSGAGG00000013279.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000020427.1">
        <property type="protein sequence ID" value="ENSGAGP00000017906.1"/>
        <property type="gene ID" value="ENSGAGG00000013279.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046404">
        <property type="term" value="F:ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051736">
        <property type="term" value="F:ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070922">
        <property type="term" value="P:RISC complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.330:FF:000001">
        <property type="entry name" value="Protein CLP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000454">
        <property type="entry name" value="Protein CLP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1030:FF:000001">
        <property type="entry name" value="Protein CLP1 homolog 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1030">
        <property type="entry name" value="Clp1, DNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.330">
        <property type="entry name" value="Pre-mRNA cleavage complex subunit Clp1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03035">
        <property type="entry name" value="Clp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028606">
        <property type="entry name" value="Clp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010655">
        <property type="entry name" value="Clp1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038238">
        <property type="entry name" value="Clp1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032324">
        <property type="entry name" value="Clp1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038239">
        <property type="entry name" value="Clp1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF6">
        <property type="entry name" value="POLYRIBONUCLEOTIDE 5'-HYDROXYL-KINASE CLP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06807">
        <property type="entry name" value="Clp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16573">
        <property type="entry name" value="CLP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Clp1 N-terminal" evidence="9">
        <location>
            <begin position="16"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 P-loop" evidence="10">
        <location>
            <begin position="121"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 C-terminal" evidence="8">
        <location>
            <begin position="313"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="124"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="425" mass="47556" checksum="A621758B80B0AAEC" modified="2019-05-08" version="1">MAEEAGDEKKQVAKFELERETELRFEVEASQTVQLELLSGMAEIFGTELTRNKKFTFDAGAKVAVFTWHGCTVQLSGRTEVAYISKDTPMLLYLNTHTALEQMRRQAEREDERGPRVMVVGPTDVGKSTVCRLLLNYAVRLGRRPTFVELDVGQGSVSIPGTMGALYIERPADVEEGFSVQAPLVYHFGSTTPGTNIKLYNKITSRLADVFNQRCEVNRRASVSGCVINTCGWVKGSGYHALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLPKSGGVVERSKDFRRECRDDRIREYFYGFRGCFYPHAFDVKFSDVKIYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRDMVHHLLSVSTADGSEENISETSVAGFIVVTGVDVDRQVFTVLSPAPRPLPKNFLLIMDIRFMDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTN8</accession>
    <name>A0A452HTN8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HTN8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018487"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021075.1">
        <property type="protein sequence ID" value="ENSGAGP00000018487.1"/>
        <property type="gene ID" value="ENSGAGG00000013689.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061343">
        <property type="term" value="P:cell adhesion involved in heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007508">
        <property type="term" value="P:larval heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395:SF22">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395">
        <property type="entry name" value="TRANSCRIPTASE, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15746" checksum="B318614FEFF1B737" modified="2019-05-08" version="1">TSSLSIMISQILDCDIPEGIYKVENEVGSEAKIGEEQVKNYLDKLDVFKSPEPDERHPRILKELTEEIYEPLAIISEKSWKTGEIPEDWKRANTVPIYKKRNKDNLGNYRPLSLTSVLGKRMEQIIKQSVCKNLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HU44</accession>
    <name>A0A452HU44_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HU44"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018660"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021274.1">
        <property type="protein sequence ID" value="ENSGAGP00000018660.1"/>
        <property type="gene ID" value="ENSGAGG00000013813.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19697">
        <property type="entry name" value="bHLH-PAS_NPAS4_PASD10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056192">
        <property type="entry name" value="bHLH_NPAS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF24">
        <property type="entry name" value="NEURONAL PAS DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23183">
        <property type="entry name" value="bHLH_NPAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="11">
        <location>
            <begin position="69"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="385"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="665"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="738" mass="81262" checksum="8F3617874816EED0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HU91</accession>
    <name>A0A452HU91_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SMB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HU91"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018692"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021309.1">
        <property type="protein sequence ID" value="ENSGAGP00000018692.1"/>
        <property type="gene ID" value="ENSGAGG00000013829.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000019">
        <property type="entry name" value="Thrombospondin type 1 domain containing 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039942">
        <property type="entry name" value="SBSPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056801">
        <property type="entry name" value="SBSPON_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036024">
        <property type="entry name" value="Somatomedin_B-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044004">
        <property type="entry name" value="TSP1_spondin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20920">
        <property type="entry name" value="RPE-SPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20920:SF2">
        <property type="entry name" value="SOMATOMEDIN-B AND THROMBOSPONDIN TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25031">
        <property type="entry name" value="SBSPON_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19028">
        <property type="entry name" value="TSP1_spondin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90188">
        <property type="entry name" value="Somatomedin B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00524">
        <property type="entry name" value="SMB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019542910" description="SMB domain-containing protein" evidence="4">
        <location>
            <begin position="27"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="5">
        <location>
            <begin position="30"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="270" mass="30533" checksum="EE4610EA19498E33" modified="2019-05-08" version="1">MSARAPASVLWLVLLALWRLWHRAEAGCSEERRCCPGRDPSCASSGWRLDRVYGTCFCDEACKLTGDCCYDYSQACPALPCIVGEWSHWSGCAEQCKPNLRMRRRYVKQEPKNGGEPCPLLEEKSGCLEYLTYQGQDCGHEHVPAFITTSEYSKERKRRAVSPLWSSDTEEAGYCVEFKTESLSHHCTVETRPYARWMQYLREGYTVCVACQPPAMNTGNHRCSGDGLGSDGNKVLRWQAVGNPRCQGTWKKVRQVEQCSCPSVHSFIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HX24</accession>
    <name>A0A452HX24_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein piccolo</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aczonin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Presynaptic active zone</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000019729"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022477.1">
        <property type="protein sequence ID" value="ENSGAGP00000019729.1"/>
        <property type="gene ID" value="ENSGAGG00000014550.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048788">
        <property type="term" value="C:cytoskeleton of presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098982">
        <property type="term" value="C:GABA-ergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098882">
        <property type="term" value="F:structural constituent of presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904071">
        <property type="term" value="P:presynaptic active zone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035418">
        <property type="term" value="P:protein localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04031">
        <property type="entry name" value="C2A_RIM1alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06714">
        <property type="entry name" value="PDZ_RIM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000137">
        <property type="entry name" value="Piccolo presynaptic cytomatrix protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000149">
        <property type="entry name" value="Piccolo presynaptic cytomatrix protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052098">
        <property type="entry name" value="Presynaptic_Scaffold_Bsn/Pclo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14113">
        <property type="entry name" value="PICCOLO/BASSOON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14113:SF6">
        <property type="entry name" value="PROTEIN PICCOLO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PDZ" evidence="15">
        <location>
            <begin position="3056"/>
            <end position="3150"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="3253"/>
            <end position="3384"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="3627"/>
            <end position="3724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="137"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="955"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1070"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1111"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1966"/>
            <end position="1985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2000"/>
            <end position="2058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2118"/>
            <end position="2185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2215"/>
            <end position="2252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2265"/>
            <end position="2294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2428"/>
            <end position="2454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2839"/>
            <end position="2911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2952"/>
            <end position="2974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="3199"/>
            <end position="3252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="3392"/>
            <end position="3468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="3545"/>
            <end position="3617"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1767"/>
            <end position="1794"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1844"/>
            <end position="1871"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="2319"/>
            <end position="2356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="241"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="263"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="322"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="336"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="390"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="962"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1113"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="2032"/>
            <end position="2041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="2123"/>
            <end position="2143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="2847"/>
            <end position="2866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="2900"/>
            <end position="2911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="3210"/>
            <end position="3233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="3242"/>
            <end position="3252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="3399"/>
            <end position="3409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="3438"/>
            <end position="3448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="3456"/>
            <end position="3468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="3563"/>
            <end position="3591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="3603"/>
            <end position="3613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="3724" mass="411759" checksum="33BE0586DE1B4E17" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HX25</accession>
    <name>A0A452HX25_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HX25"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019679"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022419.1">
        <property type="protein sequence ID" value="ENSGAGP00000019679.1"/>
        <property type="gene ID" value="ENSGAGG00000014520.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000022424.1">
        <property type="protein sequence ID" value="ENSGAGP00000019683.1"/>
        <property type="gene ID" value="ENSGAGG00000014520.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057598">
        <property type="entry name" value="Fn3_PTPRU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051622">
        <property type="entry name" value="R-tyr_protein_phosphatases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051:SF5">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23144">
        <property type="entry name" value="Fn3_PTPRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0768">Sushi</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="839"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="11">
        <location>
            <begin position="20"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="11">
        <location>
            <begin position="83"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="11">
        <location>
            <begin position="143"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="11">
        <location>
            <begin position="203"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="11">
        <location>
            <begin position="263"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="904" mass="101121" checksum="FF13B5CC9E82BA44" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXG8</accession>
    <name>A0A452HXG8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Fidgetin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HXG8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019881"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022654.1">
        <property type="protein sequence ID" value="ENSGAGP00000019881.1"/>
        <property type="gene ID" value="ENSGAGG00000014642.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008568">
        <property type="term" value="F:microtubule severing ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19523">
        <property type="entry name" value="RecA-like_fidgetin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000022">
        <property type="entry name" value="Fidgetin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000495">
        <property type="entry name" value="Fidgetin like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047828">
        <property type="entry name" value="Fidgetin_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF14">
        <property type="entry name" value="FIDGETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="507"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="194"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="321"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="321"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="370"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="748" mass="81136" checksum="F9F149AFC1AB43D8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYS6</accession>
    <name>A0A452HYS6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HYS6"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023172.1">
        <property type="protein sequence ID" value="ENSGAGP00000020336.1"/>
        <property type="gene ID" value="ENSGAGG00000014993.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="90" mass="10640" checksum="B0775345D739F778" modified="2019-05-08" version="1">MFLSALVDWMIPDIPKDISEQIKKEKSMLVDFFLKEEHEKLKLIESFIMRDKHRNKGETKGRRNRAASFCQFSRSQRGSFTSFSSQHTEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYS9</accession>
    <name>A0A452HYS9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYS9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020328"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023164.1">
        <property type="protein sequence ID" value="ENSGAGP00000020328.1"/>
        <property type="gene ID" value="ENSGAGG00000014988.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15225">
        <property type="entry name" value="7tmA_OR10A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="203"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="47"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="321" mass="36374" checksum="FD441D732EA5069D" modified="2019-05-08" version="1">MKTRQETVGRNSTTITGFILLGFSDIPSLQHLFFWVFLVIYIVILAGNLLIIVLTLADPALHTPMYFFLRNLSFLEMCYTSVNVPKMLRNVLSWDKTISFIGCAMQTYFTLFLGGSECFLLASMAYDRYVAICKPLHYPVVMNRKVSTGLVIGSWLSGLLVYFGLTSMVFILPFCRSHEINHFFCDIPPLLKLACGDTSRNEMVVFMVALFFVTFPFMLILMSYVCIISTILRVPSGAGRRKSFSTCSSHLMVVTLFFGSVSIMYLKPNSSYSPDTDKFLSLSYTIITPLLNPIIYSLRNKEVKAKFLLLTRHRQASLCPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZ38</accession>
    <name>A0A452HZ38_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">THAP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HZ38"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020504"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023357.1">
        <property type="protein sequence ID" value="ENSGAGP00000020504.1"/>
        <property type="gene ID" value="ENSGAGG00000015096.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021896">
        <property type="entry name" value="THAP9-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055035">
        <property type="entry name" value="THAP9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048366">
        <property type="entry name" value="TNP-like_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048367">
        <property type="entry name" value="TNP-like_RNaseH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048365">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47577:SF2">
        <property type="entry name" value="THAP DOMAIN CONTAINING 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47577">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22824">
        <property type="entry name" value="THAP9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21788">
        <property type="entry name" value="TNP-like_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21789">
        <property type="entry name" value="TNP-like_RNaseH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21787">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12017">
        <property type="entry name" value="Tnp_P_element"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="875" mass="99440" checksum="D4668BF7CD032FED" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0I4</accession>
    <name>A0A452I0I4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I0I4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020959"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023873.1">
        <property type="protein sequence ID" value="ENSGAGP00000020959.1"/>
        <property type="gene ID" value="ENSGAGG00000015403.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019449121" description="KRAB domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="2">
        <location>
            <begin position="38"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="83" mass="9246" checksum="E64BE91F30198734" modified="2019-05-08" version="1">MCIPELLFTWCCCSVNSGVVSGAAGTECWTLFLFQGPMTFEEVAVYFTREEWALLDPGQRAVHRDLSPCPDLNSITFPSLNKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1H6</accession>
    <name>A0A452I1H6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I1H6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021320"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024284.1">
        <property type="protein sequence ID" value="ENSGAGP00000021320.1"/>
        <property type="gene ID" value="ENSGAGG00000015655.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15231">
        <property type="entry name" value="7tmA_OR5V1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="320" mass="35643" checksum="69696E514ACC0A18" modified="2019-05-08" version="1">MGNQTHIMEFIFLGFSNHPNLQMLFFLVFLVIYTVTLLGNTLILTLTRADPTLHTPMYFFLSNLSFLDICYTSATVPVMLVNFFRHRKTISYAGCMAQLFFLITCAGTECVLLAVMAYDRYVAVCKPLSYPSIMSNRLCVQLAGFSWLCGLVNSLVHTVLTSAIVICKSNQLSHFFCDVPLLLKISCTDTSVNEAVLHLASALIGLSPCLFTVVSYGHIISAILKIQSIKGRFKAFSTCTSHLIVVIIFYSTSNFNYNRPSLGYSLDIDTLVSSLYCIVTPMLNPVIYSLRNKEVKEPSSHLSCLVTSYLLSDLIKKSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2Q3</accession>
    <name>A0A452I2Q3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Ubiquitin carboxyl-terminal hydrolase 39</fullName>
            <ecNumber evidence="4">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">U4/U6.U5 tri-snRNP-associated 65 kDa protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">The U4/U6-U5 tri-snRNP complex is a building block of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452I2Q3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021813"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024848.1">
        <property type="protein sequence ID" value="ENSGAGP00000021813.1"/>
        <property type="gene ID" value="ENSGAGG00000016011.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02669">
        <property type="entry name" value="Peptidase_C19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000068">
        <property type="entry name" value="U4/U6.U5 tri-snRNP-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000030">
        <property type="entry name" value="U4/U6.U5 tri-snRNP-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033809">
        <property type="entry name" value="USP39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF16">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="16" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="25">
        <location>
            <begin position="89"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="24">
        <location>
            <begin position="211"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00079185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="551" mass="63918" checksum="B902C824B3571F2F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3Q7</accession>
    <name>A0A452I3Q7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Centrosomal protein of 78 kDa</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CEP78 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3Q7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022155"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025254.1">
        <property type="protein sequence ID" value="ENSGAGP00000022155.1"/>
        <property type="gene ID" value="ENSGAGG00000016274.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000057">
        <property type="entry name" value="Centrosomal protein of 78 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000070">
        <property type="entry name" value="Centrosomal protein of 78 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026212">
        <property type="entry name" value="Cep78"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24110">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 78 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24110:SF3">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 78 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02062">
        <property type="entry name" value="CENTROSOME78"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="582"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="647"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="458"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="582"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="669"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="682"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="740"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="762"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="771" mass="85611" checksum="3666E2582527A1EE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I428</accession>
    <name>A0A452I428_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I428"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022292"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025407.1">
        <property type="protein sequence ID" value="ENSGAGP00000022292.1"/>
        <property type="gene ID" value="ENSGAGG00000016377.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000025408.1">
        <property type="protein sequence ID" value="ENSGAGP00000022293.1"/>
        <property type="gene ID" value="ENSGAGG00000016377.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000003">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.320:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.30:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.320">
        <property type="entry name" value="Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038502">
        <property type="entry name" value="M1_LTA-4_hydro/amino_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034015">
        <property type="entry name" value="M1_LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015211">
        <property type="entry name" value="Peptidase_M1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726:SF2">
        <property type="entry name" value="AMINOPEPTIDASE RNPEPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09127">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01263">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal" evidence="9">
        <location>
            <begin position="297"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634015-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="486" mass="55250" checksum="AB8F38CE2381C4EF" modified="2019-05-08" version="1">MSATQSTYLEQEGIYQFYMEYPVPAYLVALVAGDLISADVGPRSRVWAEPCLLPTAISKLSGMVEHWLSAAESLYGRYIWGRYDIVFLPPSFPIVAMENPCLTFIISSILESDDFLIIDVIHEIAHSWFGNAVTNATWEEMWLSEGLATYAQRRITTETYGAAFTCLETAFRLDALHRQMKLLGEDNPVSKLQVKLEPGVNPSNMMNLFTYEKGYCFVYYLSQLCGDPKHFDSFLRAYIEKYKFTSVVAQDLLDSFLNFFPELKEQSVDNKAGLEFERWLNATGPPLAEPDLSQGSSLIRPVETLFKLWTTEPLDAVAAASSVDITKWRPFQTVLFLDRLLDGSPLPPEVVKKLSECYSSQLDTMNAEIRIRWLQIVVRNDYYPDFYKVRHFLEYQMSRMYTIPLYEDICTGTLKSFALEVFYQTQNQLHPNLRKTIQQILTQGLNPLPTTDTTAVATDTPTVVLEDKPSETANSAISLRDVNVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I782</accession>
    <name>A0A452I782_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine/arginine repetitive matrix protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I782"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023482"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026765.1">
        <property type="protein sequence ID" value="ENSGAGP00000023482.1"/>
        <property type="gene ID" value="ENSGAGG00000017218.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000026766.1">
        <property type="protein sequence ID" value="ENSGAGP00000023483.1"/>
        <property type="gene ID" value="ENSGAGG00000017218.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042551">
        <property type="term" value="P:neuron maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029360">
        <property type="entry name" value="SRRM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052109">
        <property type="entry name" value="SRRM_Domain-Containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34755">
        <property type="entry name" value="SERINE/ARGININE REPETITIVE MATRIX PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34755:SF1">
        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15230">
        <property type="entry name" value="SRRM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/arginine repetitive matrix protein C-terminal" evidence="2">
        <location>
            <begin position="465"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="397"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="418"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="527"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="561"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="600"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="611" mass="68839" checksum="78B1D41162365E8E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7R9</accession>
    <name>A0A452I7R9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I7R9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023679"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026976.1">
        <property type="protein sequence ID" value="ENSGAGP00000023679.1"/>
        <property type="gene ID" value="ENSGAGG00000017342.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10780">
        <property type="entry name" value="MITOCHONDRIAL CARRIER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10780:SF20">
        <property type="entry name" value="MITOCHONDRIAL CARRIER HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="116"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="282" mass="30823" checksum="72C2ED76196F8B4B" modified="2019-05-08" version="1">MADSASQVLLGSGLTALSQPLMYVKVLVQVGYEPLPPTLGRNLFGRQVYQLPGLFAYAKHIMKIDGRAGLFKGLAPRLCSGAIGTIVHSQVLQRYQEADQEEEPGSSLKEPLSSLEQVIKETSREMVARSAATLVTHPFHVITLRCMVQFIGRETKYSGIFSPFVTIYREEGILGFFAGLVPRLLGDVLSLWLCNMLAYLINTYALENGVSAISEMKSYSQAVTGFFASMLTYPFVLVSNLMAINNCGLAGGLPPYAPNYSSWLDCWNQLLKEVCPGLAPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8X0</accession>
    <name>A0A452I8X0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I8X0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024101"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027445.1">
        <property type="protein sequence ID" value="ENSGAGP00000024101.1"/>
        <property type="gene ID" value="ENSGAGG00000017631.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000018">
        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF118">
        <property type="entry name" value="PROTOCADHERIN BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019396637" description="Cadherin domain-containing protein" evidence="14">
        <location>
            <begin position="29"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="688"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="134"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="244"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="349"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="454"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="570"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="804" mass="88986" checksum="6A7593EB8E52B88D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9K3</accession>
    <name>A0A452I9K3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I9K3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024364"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027733.1">
        <property type="protein sequence ID" value="ENSGAGP00000024364.1"/>
        <property type="gene ID" value="ENSGAGG00000017802.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05716">
        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF87">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019217606" description="Ig-like domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="5"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="225" mass="24601" checksum="A1C824DF574F17B8" modified="2019-05-08" version="1">MRIVPVLVWILFPGCWAVTGPGTAHGQLGGSVSVECQYGAGYEAFPKFWCKRKVVLCFNHLIFETTGLEAEETWGRVSIRDNHTQRVITVTLENLTLADAGTYLCGVARIGLPNPRESVKVIISPAAALPSSISTEKAPQATDQPAAPAFTWISTGSHVSSPFTASSNSCLDRSDIQAQTQPNILYPLFLIVPIFLCIVCAVIWVSIQYRRNSREMVPNRHGESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAH9</accession>
    <name>A0A452IAH9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mineralocorticoid receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IAH9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024536"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027920.1">
        <property type="protein sequence ID" value="ENSGAGP00000024536.1"/>
        <property type="gene ID" value="ENSGAGG00000017921.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="605" mass="64342" checksum="6C48EA53EC11EF09" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAL3</accession>
    <name>A0A452IAL3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TAFII55 protein conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IAL3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024741"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028152.1">
        <property type="protein sequence ID" value="ENSGAGP00000024741.1"/>
        <property type="gene ID" value="ENSGAGG00000018060.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08047">
        <property type="entry name" value="TAF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037817">
        <property type="entry name" value="TAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006751">
        <property type="entry name" value="TAFII55_prot_cons_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228:SF0">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID 55 KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04658">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01370">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII55 protein conserved region" evidence="7">
        <location>
            <begin position="19"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="195"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="346"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="360" mass="41406" checksum="09D4D3EB629A84EB" modified="2019-05-08" version="1">MTSTKLKMSKNKDDAPHELESQFILRLPPEYASTVRRAVQSGSVNLKDRLTIELHPDGRHGIVRVDRVPLAAKLVDLPCITESLKTIDKKTFYKTADICQMLVCTVDGDLYPPLEEPTVSTDPKANKKKDKDREKKFIWNHGITLPLKNVRKRRFRKTAKKKYIESPDVEKEVKRLLSTDAEAVSVRWEVIAEDETKEADNHGSLTGLDISSPGMSGHKQGHGSSEHDELREIFNDISSSSEDEDERDHHDDEDLNIMDTEEDLERQLQDKLNESDGQQQENEGTNQIAMGIQKQIDNLKGKLQETQERRKRQEDLIMKVENLALKTRLQAVLDEFRQQEDREKQQLTSLQEQLESLMEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB64</accession>
    <name>A0A452IB64_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prune exopolyphosphatase 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IB64"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024973"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028431.1">
        <property type="protein sequence ID" value="ENSGAGP00000024973.1"/>
        <property type="gene ID" value="ENSGAGG00000018263.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1640.10:FF:000003">
        <property type="entry name" value="Prune homolog 2 with BCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF49">
        <property type="entry name" value="DHHA2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="31428" checksum="FF12BEAD2231C3A4" modified="2019-05-08" version="1">MLIEKFLQLCKTFRYFNCKRLGRVHVVLGNKPCDLDSLISALTYAYFLDKVSPPDVLCLPVLNIPRREFSYYPETRFILEELNIPESFHIFQDEINLHQLNDEGKLSLTLVNSNMLASDDKSLESAVVKVINPDERCDVNLGLQASSSSLVVKEILQEAPELITQQLAHLLRGSILFQCMSVEPERITEQQEEILSILEEKFPELPPREDIISVFQQTPFKAQGLNIEEVMLKDLKELSDGEIKVAVITMLCSNTSRTLSGSQCLTEAHKRTVVGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICG0</accession>
    <name>A0A452ICG0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ICG0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025493"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029014.1">
        <property type="protein sequence ID" value="ENSGAGP00000025493.1"/>
        <property type="gene ID" value="ENSGAGG00000018526.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034334">
        <property type="term" value="P:adherens junction maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032233">
        <property type="term" value="P:positive regulation of actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22060">
        <property type="entry name" value="WH2_MTSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030127">
        <property type="entry name" value="MTSS1/MTSS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708">
        <property type="entry name" value="ACTIN BUNDLING/MISSING IN METASTASIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708:SF10">
        <property type="entry name" value="PROTEIN MTSS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08397">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51338">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="2">
        <location>
            <begin position="683"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="594"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="178"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="270"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="569"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="711" mass="77126" checksum="842F0DFEE17FEAC7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICQ5</accession>
    <name>A0A452ICQ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ICQ5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025478"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028998.1">
        <property type="protein sequence ID" value="ENSGAGP00000025478.1"/>
        <property type="gene ID" value="ENSGAGG00000018623.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042352">
        <property type="entry name" value="EFCAB14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15717:SF2">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15717">
        <property type="entry name" value="PROTEIN KIAA0494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="436"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="385"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="497" mass="55128" checksum="7C1E6F3888AD8C0F" modified="2019-05-08" version="1">MKKRKELNALIGLAGDGRRKKLPKKGSGHRLLRTEPPASDSESSSEGDEDEFGPPGTGSRFAKGDYLRCCKFCYPLCAFVILAACVVACVGLVWMQVALKEDLDALKEKFRTMESYQKTSFQEIPKLNEDLLDKQKHLEKIETGDMGLNKIWINITEINKQILFLTSAVNHLKVNIKSASDLITLPVTVEELQKSVATIGSTLTSVAHDVDAMQVTIENHRKTTEKLQNDLNEYIKENGEKQISSLSTTVSPRISNRNQTENCKQDNQFLHATVEEINASLVVYQKLNDLKLLSVDTAISNITQRVAWLESSVVTVNALENRANLSTSAVGNTTTSTKVESQDQPENETATEKAQNAKKIENVDPQVSKLREKLQLINALTSKPENEKSLEASIKGGKMQTATSKPTNLPKLLSRSVGGNMERNGHPRRLSLPGIATIKDLQDLFEKTDQGTDGKLSYEDLQTLVGLAIQEPQSFKEFDTDRDEKYSLPELRLALGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDD9</accession>
    <name>A0A452IDD9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDD9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025860"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029416.1">
        <property type="protein sequence ID" value="ENSGAGP00000025860.1"/>
        <property type="gene ID" value="ENSGAGG00000018885.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000706">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000759">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000551">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF52">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF26.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="390"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="102"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="477" mass="53656" checksum="1C45FE840C11BFFC" modified="2019-05-08" version="1">MGTRCPPAIDSQCRSLEVPASGNAAWHPWDLPLISSAWEQLLSLLRLGTNGCATERLGSWHLAAFCDEHWKFPCPRTEPSIHDWKVQHTPGNITALQRNVVAPEGPQTNPEPKGKVPTAPTSSPSQKSLSASDRGGAVATSTEPMNFQHRGNLITHLRVHTGEKPFTCTECGKSFSQKGDLMRHQRIHTGEKPFECNVCGKSFCSKQTFILHQRIHTGEKPFSCTECGKCFNRKANFITHQKIHRGERPFVCAECGKGFCAKKTFILHQKIHIGDRPFGCSECGKSFSRNGDLTRHQRIHTGERPFACADCGKCFSHNGELIKHQRIHTGEKPFSCTECGKSFNRKGTLITHQRIHTGERPFVCAECGKTFNLKTTLMKHKRIHTGERPFTCVECGKSFKYKGNLRTHHLTHTVERVYPCTECGKIFSHKKELTMHQAVHTEERLLSCYGDGESFNPFLQMHPLLLSVPQAKCLWKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFD4</accession>
    <name>A0A452IFD4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Synaptosomal-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Synapse</location>
            <location evidence="6">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the SNAP-25 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IFD4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026438"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030052.1">
        <property type="protein sequence ID" value="ENSGAGP00000026438.1"/>
        <property type="gene ID" value="ENSGAGG00000019263.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031629">
        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15884">
        <property type="entry name" value="SNARE_SNAP23C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000018">
        <property type="entry name" value="Synaptosomal-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000928">
        <property type="entry name" value="SNAP-25_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF22">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00835">
        <property type="entry name" value="SNAP-25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="2" id="KW-0771">Synaptosome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="154"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="220" mass="23876" checksum="6BD16F08F2D5F2F6" modified="2019-05-08" version="1">MQPPHTPASQGAWGARGSPVRSDTSQVWGATLWDQTLAGFGGRPVRSDPSRVWGQPCGIRLQLGSGATLWGLTPAKEKGGPGCPGWGQTPQACVPLAPPILVLLRLKNIKASEAYKAVWGNTQDGVVSTQPSRVADEREQMIMSGDYIHRITNDAREDEMEENLVQVGSILGNLKSMALDVGNEIDIQNKQVDKIMEKVTVNEDRIQEANTRAKKILKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHN0</accession>
    <name>A0A452IHN0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IHN0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026999"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030681.1">
        <property type="protein sequence ID" value="ENSGAGP00000026999.1"/>
        <property type="gene ID" value="ENSGAGG00000019645.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000166">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF47">
        <property type="entry name" value="KALLIKREIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019271787" description="Peptidase S1 domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="28"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="184" mass="20654" checksum="6F32604B57E6F6EB" modified="2019-05-08" version="1">MTWWFGCSAGTWKTWLLISASDTEGNQVTGGSFCLFRSRPYQVVLLRNGRIYCVGSLIAPKWVLTAAHCGRRVRRISSLRVHLGDYKLPAKEGTEQIRRIHNFFMHPGYNLHPRDNDFMLLELDEPAQLNNYVNTINMTTHCPSPGTRCSVSGWGTIKAWVSHNSPAAEQADKDTISLNVCEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHS4</accession>
    <name>A0A452IHS4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4939 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IHS4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027125"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030820.1">
        <property type="protein sequence ID" value="ENSGAGP00000027125.1"/>
        <property type="gene ID" value="ENSGAGG00000019729.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032549">
        <property type="entry name" value="DUF4939"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16297">
        <property type="entry name" value="DUF4939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4939" evidence="1">
        <location>
            <begin position="25"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="140" mass="16370" checksum="CE9A3A9B35873337" modified="2019-05-08" version="1">MAFEEWRLPLEGAQFQVQVLMDHKNLESLQMARHHNQLTQAPPELQIPIPDKFDSDHRKFHKFLNQCPLLFLMDLQFLPTDRTRVGLIINLLAGEALAWASQLLEKSSPLLKYLQKNLEKGLTLLIRIWEGDEWKSAFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJE8</accession>
    <name>A0A452IJE8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJE8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027956"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031755.1">
        <property type="protein sequence ID" value="ENSGAGP00000027956.1"/>
        <property type="gene ID" value="ENSGAGG00000020306.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13954">
        <property type="entry name" value="7tmA_OR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="147"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="198"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="308" mass="33944" checksum="0D05B05F9903F38D" modified="2019-05-08" version="1">MGKENNTSVTEFILLGLSSNPAEELVLFEVFTAIYLVALMGNVLILLLVSLDSQLHMPMYFLLGNLSVVDIGYTSSTVPRMLANYLLGDKSISWAGCLSQMFFFISFGGVECLILGVMVYDCYLAICHPLHYGMFMSPRVCTLLGEAAWVMGLTNSAVHSSLMSILSFCWGNILHHFFCDIPPLFQLSCSDTRANQAVTIVVGGAIIFSSLLGTLVSYVHITMAVLRICTREESLNAFSTCASHLTVVSLYFGTILFTYLCPNSTYSQEQDRALPVLYGIIIPMLNPIIYSLRNKDVKGDLQKSLARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKD6</accession>
    <name>A0A452IKD6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C-X-C chemokine receptor type 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IKD6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028428"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032291.1">
        <property type="protein sequence ID" value="ENSGAGP00000028428.1"/>
        <property type="gene ID" value="ENSGAGG00000020612.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016494">
        <property type="term" value="F:C-X-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042113">
        <property type="term" value="P:B cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15181">
        <property type="entry name" value="7tmA_CXCR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000143">
        <property type="entry name" value="C-X-C chemokine receptor type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001053">
        <property type="entry name" value="Chemokine_CXCR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF618">
        <property type="entry name" value="C-X-C CHEMOKINE RECEPTOR TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00564">
        <property type="entry name" value="CXCCHMKINER5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0075">B-cell activation</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="71"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="372" mass="42354" checksum="E900B00235103A37" modified="2019-05-08" version="1">MGPYSISMKSYDMNLLGEFSDYNDNDNTTRSYDDYFCPETVSSLASDPTHPFKKIFMPLVYLLIFLLGTLGNALVLIILERYKRARTTTENFLFHLALANLVLLLTLPFGVTESLTGWIFGTFLCKVLSAVHKINFYCSSMLLGCISVDRYLAIVYAIHTYRKRRVRSIHLTCLAIWFVSLVLTLPDLVLMEVWSDKSNHSVCNFQQVGIHGSNMWLATRFLYHIVGFFLPLLVMCYCYTAIVRTLCQSQRLQRQKAVRVAILVTGVFLLCWSPYHMVIFLDTLDKLETLPNDCALADQLGTSIMVTEVIGFTHCCLNPILYAFVGVKFRNDFFRILRDLGCISQETLQEILDVTRKGSGIESDTITSVSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPY3</accession>
    <name>A0A452IPY3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Structural maintenance of chromosomes protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Forms a heterodimer with smc6. Component of the SMC5-SMC6 complex which consists at least of smc5, smc6, nsmce2, nsmce1 and nsmce4a.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC5 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IPY3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029969"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034036.1">
        <property type="protein sequence ID" value="ENSGAGP00000029969.1"/>
        <property type="gene ID" value="ENSGAGG00000021632.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001301">
        <property type="entry name" value="Structural maintenance of chromosomes 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000793">
        <property type="entry name" value="Structural maintenance of chromosomes protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="14">
        <location>
            <begin position="49"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="216"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="387"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="677"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="869"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1079" mass="126336" checksum="4DF79C2ABA0BDAEE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQK1</accession>
    <name>A0A452IQK1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rho-associated protein kinase 2</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Rho-associated, coiled-coil-containing protein kinase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Rho-associated, coiled-coil-containing protein kinase II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">p164 ROCK-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="38 39">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="39" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="38" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030269"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034355.1">
        <property type="protein sequence ID" value="ENSGAGP00000030269.1"/>
        <property type="gene ID" value="ENSGAGG00000021817.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000034358.1">
        <property type="protein sequence ID" value="ENSGAGP00000030271.1"/>
        <property type="gene ID" value="ENSGAGG00000021817.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072518">
        <property type="term" value="F:Rho-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010825">
        <property type="term" value="P:positive regulation of centrosome duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901888">
        <property type="term" value="P:regulation of cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006939">
        <property type="term" value="P:smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11638">
        <property type="entry name" value="HR1_ROCK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01242">
        <property type="entry name" value="PH_ROCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22250">
        <property type="entry name" value="ROCK_SBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000047">
        <property type="entry name" value="Rho-associated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000016">
        <property type="entry name" value="Rho-associated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.730:FF:000001">
        <property type="entry name" value="rho-associated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000072">
        <property type="entry name" value="Rho-associated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001759">
        <property type="entry name" value="Rho-associated, coiled-coil-containing protein kinase 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.730">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057529">
        <property type="entry name" value="MRCK/ROCK_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037311">
        <property type="entry name" value="ROCK2_HR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015008">
        <property type="entry name" value="ROCK_Rho-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF28">
        <property type="entry name" value="RHO-ASSOCIATED PROTEIN KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25346">
        <property type="entry name" value="PH_MRCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08912">
        <property type="entry name" value="Rho_Binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103652">
        <property type="entry name" value="G protein-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51859">
        <property type="entry name" value="RHO_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 31" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="21" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20 30 32" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="23" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="22" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 31" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="39" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0862">Zinc</keyword>
    <keyword evidence="15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Protein kinase" evidence="34">
        <location>
            <begin position="94"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="35">
        <location>
            <begin position="359"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="37">
        <location>
            <begin position="503"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="RhoBD" evidence="36">
        <location>
            <begin position="985"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="32">
        <location>
            <begin position="441"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="32">
        <location>
            <begin position="647"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="32">
        <location>
            <begin position="1062"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00030038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00031784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00032261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS51859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1316" mass="152205" checksum="39ED226FE7045F3E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQN3</accession>
    <name>A0A452IQN3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ras-related and estrogen-regulated growth inhibitor</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452IQN3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030301"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034390.1">
        <property type="protein sequence ID" value="ENSGAGP00000030301.1"/>
        <property type="gene ID" value="ENSGAGG00000021838.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04146">
        <property type="entry name" value="RERG_RasL11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000942">
        <property type="entry name" value="Ras-related and estrogen-regulated growth inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051065">
        <property type="entry name" value="Ras-related_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45704">
        <property type="entry name" value="RAS-LIKE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="199" mass="22524" checksum="5A0092F7FA381C70" modified="2019-05-08" version="1">MAKSAEVKLAIFGRAGVGKSALVVRFLTKRFIWEYDPTLESTYRHQATIDDEVVSMEILDTAGQEDTIQKEGHVRWGEGFVLVYDITDRSSFEEVLPLKNLLDEVKKPKNVTLILVGNKADLDHSRQVSTEEGEKLATELACAFYECSACTGEGNIMEAFYELCREVRRRKMVQGKTRRRSSTTHVKQAINKMLTKISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITV6</accession>
    <name>A0A452ITV6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ITV6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031320"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035524.1">
        <property type="protein sequence ID" value="ENSGAGP00000031320.1"/>
        <property type="gene ID" value="ENSGAGG00000022508.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07277">
        <property type="entry name" value="PX_RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17689">
        <property type="entry name" value="RUN_SNX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047329">
        <property type="entry name" value="RUN_SNX29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037916">
        <property type="entry name" value="SNX29_PX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47194:SF4">
        <property type="entry name" value="SORTING NEXIN-29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47194">
        <property type="entry name" value="SORTING NEXIN-29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="4">
        <location>
            <begin position="97"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="720"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="846"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="530"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="879" mass="99263" checksum="24D420253952736F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUD5</accession>
    <name>A0A452IUD5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A452IUD5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031725"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035978.1">
        <property type="protein sequence ID" value="ENSGAGP00000031725.1"/>
        <property type="gene ID" value="ENSGAGG00000022788.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030311">
        <property type="term" value="P:poly-N-acetyllactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000001">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF234">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="396" mass="45143" checksum="AF50A30CEA9F0376" modified="2019-05-08" version="1">MRLRYIKAYVWLSCVFVANVIVYTVVTLSRRPLSLQASGRSAFIGGHLRLWQHRDYHGLPLWDLLLLYMDRLENPILRNATSQFNLTLPGTCGESFHEASQRLADFSSYPSHLQAFILSMHCRDYPLLIGPADDEACPNGTQLLVAVRSQMANFDRRQAIRQTWGRTLLVRNVTVQTLFLLGKQGSEDGHPDLEALLELERQKNRDLLLWDFQDTFYNLSLKDILFLGWLRGHCPSVELIFLGDDDVFLRVETLVAYAGNFDTITSRSLFAGHVIQDAAPVHDCKHKYYVPESFYQGTYPPYLGGGGILCSRDVATRLYEASCHLPLFPISNIFLGICLRELALVPVNHPGFWTFNMEAEGQEDLCAYKELILVDHRTPQDIIRLWRGLHDPALAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IVV4</accession>
    <name>A0A452IVV4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000032027"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036311.1">
        <property type="protein sequence ID" value="ENSGAGP00000032027.1"/>
        <property type="gene ID" value="ENSGAGG00000022957.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="8102" checksum="C9088B0F69D84F99" modified="2019-05-08" version="1">MDDQPRLMHSHAGVGMAGHPGLSQHMQDGAGGTEGEGGRKQDIGDILQQIMTITDQSLXLGGGSVILLSEEKLLRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IW81</accession>
    <name>A0A452IW81_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Beta-tectorin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">One of the major non-collagenous components of the tectorial membrane. The tectorial membrane is an extracellular matrix of the inner ear that covers the neuroepithelium of the cochlea and contacts the stereocilia bundles of specialized sensory hair cells. Sound induces movement of these hair cells relative to the tectorial membrane, deflects the stereocilia and leads to fluctuations in hair-cell membrane potential, transducing sound into electrical signals.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
            <orientation evidence="1">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IW81"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032356"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036681.1">
        <property type="protein sequence ID" value="ENSGAGP00000032356.1"/>
        <property type="gene ID" value="ENSGAGG00000023154.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000006">
        <property type="entry name" value="beta-tectorin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017977">
        <property type="entry name" value="ZP_dom_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002:SF13">
        <property type="entry name" value="BETA-TECTORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002">
        <property type="entry name" value="ENDOGLIN/TGF-BETA RECEPTOR TYPE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00682">
        <property type="entry name" value="ZP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018974274" description="Beta-tectorin" evidence="14">
        <location>
            <begin position="18"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="15">
        <location>
            <begin position="19"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="330" mass="36877" checksum="8F2EC428F59C9FBD" modified="2019-05-08" version="1">MVIVALYLLDILVQAFAAPCSPNKADIILVYCYPKTIITKIPECPYGWEVNQLALGGICYNGVHDSGYYQFTIPDLSPKNKSYCGTQSEFKNPIYHFYNSIVSNDSSVIVKSQPVNYSFTCTYHANYLVNQAAFDQRVATVHVKNGSSGSFESQLSLNFYSNAKFSSKKEAPFVIETSEIGSDVFAGVEAKGLSNRFKVVLNNCWATPSSEYFYQIQWPLITKGCATDNSILVHENGKNSRATFQFNAFRFQNIPKLSKVWLHCETHVCDSEKFSCPVPCEKRKRRTEQTGGVLVAEFAVHSKCGCFLFGKEFLSLTKCHRGSQSFSGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IX75</accession>
    <name>A0A452IX75_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Beta-ureidopropionase</fullName>
            <ecNumber evidence="9">3.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Beta-alanine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">N-carbamoyl-beta-alanine amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes a late step in pyrimidine degradation. Converts N-carbamoyl-beta-alanine (3-ureidopropanoate) into beta-alanine, ammonia and carbon dioxide. Likewise, converts N-carbamoyl-beta-aminoisobutyrate (3-ureidoisobutyrate) into beta-aminoisobutyrate, ammonia and carbon dioxide.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; beta-alanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer, homotetramer, homooctamer; can also form higher homooligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the carbon-nitrogen hydrolase superfamily. BUP family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.6" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452IX75"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032624"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036976.1">
        <property type="protein sequence ID" value="ENSGAGP00000032624.1"/>
        <property type="gene ID" value="ENSGAGG00000023319.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003837">
        <property type="term" value="F:beta-ureidopropionase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033396">
        <property type="term" value="P:beta-alanine biosynthetic process via 3-ureidopropionate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07587">
        <property type="entry name" value="ML_beta-AS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000009">
        <property type="entry name" value="Beta-ureidopropionase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050345">
        <property type="entry name" value="Aliph_Amidase/BUP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674:SF2">
        <property type="entry name" value="BETA-UREIDOPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674">
        <property type="entry name" value="NITRILASE C965.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="13">
        <location>
            <begin position="71"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="383" mass="42330" checksum="E4343955394E752D" modified="2019-05-08" version="1">MAGSLDSLEKCLERHLPPEELGEVKRILYGSLSRKLALPAAALSAASQRAFELQGCGFGAAPEQLRQPRVVRVGLVQNQIPLPTDTPVAEQVAALHRRIEEIVEVAAMCGVNVICFQEAWTMPFAFCTREKLPWTEFAESAEDGLTTRFCQKLAKKHNMVVVSPILERDDVHGGTLWNTAVVISNSGSVLGKSRKNHIPRIGDFNESTYYMEGNTGHPVFQTQFGRIAVNICFGRHHPLNWLMYSLNGAEIIFNPSATTGELSESLWPIEARNAAIANHCFTCAINRVGTEYYVNAFTSGDGGKAHHDLGYFYGSSYVAAPDGSRTPGLSRTQDGLLVAEVDLNLCRQVSDKWSFKMTGRYEMYAEELMKAVQLNFAPNIIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYW1</accession>
    <name>A0A452IYW1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Inward rectifier potassium channel C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYW1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033279"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037708.1">
        <property type="protein sequence ID" value="ENSGAGP00000033279.1"/>
        <property type="gene ID" value="ENSGAGG00000023724.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF16">
        <property type="entry name" value="G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 12" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 12" id="KW-0813">Transport</keyword>
    <keyword evidence="5 12" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="13">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="106" mass="11986" checksum="CC8536703F499581" modified="2019-05-08" version="1">MTCQARTSYTEDEVLWGHRFFPVISLEEGFFKVDYSQFHATFEVPTTPYSVKEQEEMLLISSPLIGPAVSNSKIPISFNGAGFSLSTFGTQSCKLLLVHHWDYLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J012</accession>
    <name>A0A452J012_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000033695"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038165.1">
        <property type="protein sequence ID" value="ENSGAGP00000033695.1"/>
        <property type="gene ID" value="ENSGAGG00000023983.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002142">
        <property type="term" value="C:stereocilia ankle link complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032426">
        <property type="term" value="C:stereocilium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060088">
        <property type="term" value="P:auditory receptor cell stereocilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10833">
        <property type="entry name" value="PDZ1_PDZD7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10834">
        <property type="entry name" value="PDZ2_PDZD7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000090">
        <property type="entry name" value="PDZ domain containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000092">
        <property type="entry name" value="PDZ domain containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051844">
        <property type="entry name" value="USH2_Complex_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116">
        <property type="entry name" value="PDZ DOMAIN CONTAINING WHIRLIN AND HARMONIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116:SF29">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="84"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="208"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="323"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="378"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="436"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="481"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="394"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="506"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="519"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="551" mass="58913" checksum="32CD73E59034CDE0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J081</accession>
    <name>A0A452J081_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Interferon alpha/beta receptor 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J081"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033759"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038240.1">
        <property type="protein sequence ID" value="ENSGAGP00000033759.1"/>
        <property type="gene ID" value="ENSGAGG00000024030.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042018">
        <property type="term" value="F:interleukin-22 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015373">
        <property type="entry name" value="Interferon/interleukin_rcp_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050650">
        <property type="entry name" value="Type-II_Cytokine-TF_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859:SF84">
        <property type="entry name" value="INTERFERON ALPHA_BETA RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859">
        <property type="entry name" value="INTERFERON/INTERLEUKIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09294">
        <property type="entry name" value="Interfer-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01108">
        <property type="entry name" value="Tissue_fac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019437347" description="Interferon alpha/beta receptor 2" evidence="3">
        <location>
            <begin position="30"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="239"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="14"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Interferon/interleukin receptor" evidence="5">
        <location>
            <begin position="130"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="55339" checksum="EC6E590CDC19E4BA" modified="2019-05-08" version="1">MAVLIGPLSFCQLVYLSILTTILFSLTEASVINPPQKLTMNSQNFEHILSWEPGSNTTMPTYYDVMYIGLSGSENKWKIAKECSNTARLFCNLTKEYEECTYTYIAMVQSITGNRVLNSSILEFSPYRSTYLGPPAVNLTACPTCINVTVKLPATYLKESSLIDIYQKLDYTITVESFDRKEKIPIKNETSEESFSYVVGSLHSNTNYCVSVAVAASLNHHSIPSALKCIITRSNTQPGYGIIPILNGGLVSLVVGIFLLGLYKGGFICLKSKPWPKVLETTNKLHCSVHEPDPENVCSVQIMYKEMEKKEWEYSYDDSESESENSGDYTRRVILGRDPSCHAKSNTFVHQTIDCKSAESSSQATELLSSDTENSEEHQSVIRENERISRVFFHPSSEVNSSSTSELSNSACFNINLNTVMLGDPDKTWDDSATLISHQKDAVDLQDSSASDALESKNFTNTVDVKKPDCHNISHEWQNPSVSDESDASDSEYIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J2V0</accession>
    <name>A0A452J2V0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TLS1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J2V0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034643"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039218.1">
        <property type="protein sequence ID" value="ENSGAGP00000034643.1"/>
        <property type="gene ID" value="ENSGAGG00000024619.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010756">
        <property type="entry name" value="Tls1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13486:SF2">
        <property type="entry name" value="SPLICING FACTOR C9ORF78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13486">
        <property type="entry name" value="TELOMERE LENGTH AND SILENCING PROTEIN 1 TLS1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07052">
        <property type="entry name" value="Hep_59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="232"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="232"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="289" mass="33532" checksum="7E66558DF6E61DED" modified="2019-05-08" version="1">MPSGKVFRRRRADSEDEEEDEQVTEEVRLKLEEAKEVQSLRRRPNGVSAAALLVGEKLQEETTLADDPFKIKTGGMVDMKKLKERGKDRINEEEDLNLGTSFSAETNRRDEDADMMKYIETELKKRKGIVESEEQKVKLKNAEDCLYELPESIRVSSAKKTEEMLSNQMLSGIPEVDLGIDAKIKNIIFTEDAKARLLAEQQNKKKDSETSFVPTNMAVNYVQHNRFYHEELSAPVRRNKEEPKARPLRVGDTEKPEPERSPPNRKRPPNEKATDDYHYEKFKKMNRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J315</accession>
    <name>A0A452J315_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the interleukin-1 receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J315"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034798"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039392.1">
        <property type="protein sequence ID" value="ENSGAGP00000034798.1"/>
        <property type="gene ID" value="ENSGAGG00000024718.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000284">
        <property type="entry name" value="interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000004">
        <property type="entry name" value="X-linked interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF10">
        <property type="entry name" value="X-LINKED INTERLEUKIN-1 RECEPTOR ACCESSORY PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="10">
        <location>
            <begin position="147"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="433" mass="49512" checksum="BDEBB34905F92FA0" modified="2019-05-08" version="1">MLSSGNPLSIACKAFFGFSGESGPVIYWMRGEKFIEELEGHIREGEIRLLKEHLGEKEVELTLTFDAVGEADLANYTCHVENRNGRKHASVLLRKKDLIYKIELAGGLGAILLLLILLVTIYKCYNIELMLFYRQNFGGDESMDDNKEYDAYLSYTKVDPDALDCDDNEEEQFALEILPDVLEKHYGYKLFIPDRDLIPSGTYIEDLTRCVEQSRRLIIVLTPDYVLRRGWSIFEMENRLHNMLVSGEIKVILIECTDLKGKVNYHEVESLKHTIKLLSVVKWKGPKSSKLNSKFWKRLVYEMPAKKKEVLSRRQVLDSAEQGLFGDLQTVPSLAMTGASATFVASQADLANYHQTDSMQMRHYCRGYEYDMSAATMPIASISNHHTYCNIPLTLLNGQLPLNNTMKDTHEFHRNNPLLPLSSQELSFTSDIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J353</accession>
    <name>A0A452J353_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Syntaxin binding protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000034843"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039451.1">
        <property type="protein sequence ID" value="ENSGAGP00000034843.1"/>
        <property type="gene ID" value="ENSGAGG00000024753.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.830.10:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="53854" checksum="5580F5C81969DD22" modified="2019-05-08" version="1">MLGPESPGAGNGLGPSGSREGARLVLTTPLPLLQLVQALISDFQGTPTNYKAAHVFFISREYQGRKSQISKAIKTLKEINLAFLPYESQVYSLDRPQTFYNWFSPCRFWEKNKELEMMAEQIATLCETLEEYPAIRYRKAGPQGTSQLAWAVSLGQDPSAGGPDKAHSQLLIVDRSFDLVFPLLQKLTYQAMAYDLLSIENDTYKYETMGTSDSQEKEALLDEDDKLWVQLRHMHIADVSKKVTELLHAFSESKKLTTDKANTEDLSQMLKKMPQYLKELNKVTGLELXKGSVEKLCGVEQDLAMGMDVDREKIKDPMKIIIPILLDPSVQAYDKLRIILLYVLKNGNATACRGQHWGVGRALPPFPSHTLSLLPLTPSPFLPQSEQLQPERKVLPESTYQLSSWTPRLKDIMEDIIEDRLDRELWPFVSDPFPSTSSQAAVSACFGHWHKSKPAAEYRTGPRLLIYVLGGVSMSDMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J465</accession>
    <name>A0A452J465_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BolA-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Acts as a mitochondrial iron-sulfur (Fe-S) cluster assembly factor that facilitates (Fe-S) cluster insertion into a subset of mitochondrial proteins. Probably acts together with the monothiol glutaredoxin GLRX5. May protect cells against oxidative stress.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with GLRX5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J465"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035241"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039902.1">
        <property type="protein sequence ID" value="ENSGAGP00000035241.1"/>
        <property type="gene ID" value="ENSGAGG00000025051.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990229">
        <property type="term" value="C:iron-sulfur cluster assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.90:FF:000001">
        <property type="entry name" value="Transcriptional regulator BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050961">
        <property type="entry name" value="BolA/IbaG_stress_morph_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46229">
        <property type="entry name" value="BOLA TRANSCRIPTION REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46229:SF2">
        <property type="entry name" value="BOLA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="88"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00053549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00064144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="124" mass="13430" checksum="A19C806097D6062B" modified="2019-05-08" version="1">ARGPLISMGKPVESSIRAKLLQALQPVHLEVHNESSMHAVPRGSETHFKVVIASQRFAGLPLLQRHRLVNEILQAELAGPVHALSIQAKTPQQWEENQSVSQSPGCLGGSRHDPHMATKLGSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4H5</accession>
    <name>A0A452J4H5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Serine/threonine-protein kinase</fullName>
            <ecNumber evidence="19">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="19">Activated by DAG and phorbol esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 19">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A452J4H5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035342"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040020.1">
        <property type="protein sequence ID" value="ENSGAGP00000035342.1"/>
        <property type="gene ID" value="ENSGAGG00000025126.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20839">
        <property type="entry name" value="C1_PKD1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20842">
        <property type="entry name" value="C1_PKD1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01239">
        <property type="entry name" value="PH_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14082">
        <property type="entry name" value="STKc_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000151">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000056">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000137">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000007">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000019">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015727">
        <property type="entry name" value="Protein_Kinase_C_mu-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968:SF9">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000552">
        <property type="entry name" value="PKC_mu_nu_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 19 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="71"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="203"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="24">
        <location>
            <begin position="355"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="516"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="270"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="639"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="522"/>
            <end position="530"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 22">
        <location>
            <position position="545"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000552-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000552-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="845" mass="95356" checksum="0A59CDBD3DF0CCC5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J537</accession>
    <name>A0A452J537_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J537"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035474"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040169.1">
        <property type="protein sequence ID" value="ENSGAGP00000035474.1"/>
        <property type="gene ID" value="ENSGAGG00000025214.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032332">
        <property type="term" value="P:positive regulation of chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22042">
        <property type="entry name" value="HMG-box_EGL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000003">
        <property type="entry name" value="Putative transcription factor SOX-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051356">
        <property type="entry name" value="SOX/SOX-like_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45789">
        <property type="entry name" value="FI18025P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45789:SF3">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="11">
        <location>
            <begin position="583"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="583"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="114"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="380"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="555"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="746"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="213"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="480"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="568"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="762"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="790" mass="86863" checksum="2944E673F1A549E5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5T8</accession>
    <name>A0A452J5T8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ATP synthase F(1) complex subunit delta, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP synthase F1 subunit delta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">F-ATPase delta subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Subunit delta, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. With the central stalk subunit gamma, is essential for the biogenesis of F(1) catalytic part of the ATP synthase complex namely in the formation of F1 assembly intermediate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the ATP synthase complex composed at least of ATP5F1A/subunit alpha, ATP5F1B/subunit beta, ATP5MC1/subunit c (homooctomer), MT-ATP6/subunit a, MT-ATP8/subunit 8, ATP5ME/subunit e, ATP5MF/subunit f, ATP5MG/subunit g, ATP5MK/subunit k, ATP5MJ/subunit j, ATP5F1C/subunit gamma, ATP5F1D/subunit delta, ATP5F1E/subunit epsilon, ATP5PF/subunit F6, ATP5PB/subunit b, ATP5PD/subunit d, ATP5PO/subunit OSCP. ATP synthase complex consists of a soluble F(1) head domain (subunits alpha(3) and beta(3)) - the catalytic core - and a membrane F(0) domain - the membrane proton channel (subunits c, a, 8, e, f, g, k and j). These two domains are linked by a central stalk (subunits gamma, delta, and epsilon) rotating inside the F1 region and a stationary peripheral stalk (subunits F6, b, d, and OSCP). Component of a complex composed at least by ATPIF1, ATP5F1A, ATP5F1B, ATP5F1C AND ATP5F1E.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase epsilon chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J5T8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035849"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040587.1">
        <property type="protein sequence ID" value="ENSGAGP00000035849.1"/>
        <property type="gene ID" value="ENSGAGG00000025436.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12152">
        <property type="entry name" value="F1-ATPase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.440:FF:000002">
        <property type="entry name" value="ATP synthase subunit delta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.15.10:FF:000004">
        <property type="entry name" value="ATP synthase subunit delta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.440">
        <property type="entry name" value="ATP synthase delta/epsilon subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.15.10">
        <property type="entry name" value="F0F1 ATP synthase delta/epsilon subunit, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00530">
        <property type="entry name" value="ATP_synth_epsil_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036794">
        <property type="entry name" value="ATP_F1_dsu/esu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048937">
        <property type="entry name" value="ATPD_C_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01216">
        <property type="entry name" value="ATP_synt_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822">
        <property type="entry name" value="ATP SYNTHASE DELTA/EPSILON CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822:SF7">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT DELTA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21335">
        <property type="entry name" value="ATPD_C_metazoa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46604">
        <property type="entry name" value="Epsilon subunit of F1F0-ATP synthase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="Epsilon subunit of F1F0-ATP synthase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11" id="KW-0139">CF(1)</keyword>
    <keyword evidence="4" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP synthase F1 complex delta/epsilon subunit N-terminal" evidence="18">
        <location>
            <begin position="24"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="F1F0-ATP synthase delta subunit C-terminal" evidence="19">
        <location>
            <begin position="102"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00070799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF21335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="145" mass="14983" checksum="CA35EF23A071D38C" modified="2019-05-08" version="1">GPLPGTASCCRGRELLLTALPLSQVLYNGATVKQVDVPTLSGSFGILASHVPTLQVLKPGVVTVFAEDGTATKYFVSSGSITVNADCSVQLLAEEVATLDMLDLATARSNLDKALSQLAAAPDETAKVEAQINVEATEALVKALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5W3</accession>
    <name>A0A452J5W3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidyl-prolyl cis-trans isomerase D</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">40 kDa peptidyl-prolyl cis-trans isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Cyclophilin-40</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Rotamase D</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Identified in ESR1 or NR3C1/GCR steroid receptor-chaperone complexes. Found in HSP90 chaperone complexes with kinase clients LCK or EIF2AK1. Two monomers associate with one HSP90 homodimer. Interacts with HSP90AA1. Interacts with HSP90AB1; PPID and FKBP4 compete for binding to HSP90AB1 and the interaction is mutually exclusive with the PPID:HSPA8 interaction. Interacts with HSPA8; PPID and STIP1 but not FKBP4 compete for binding to HSPA8 and the interaction is mutually exclusive with the PPID:HSP90AB1 interaction. Interacts with S100A1 and S100A2; the interactions dissociate the PPID:HSP90AA1 interaction. Interacts with S100A6. Interacts with MYB, ILF2, XRCC6, RACK1 and RPS3. Interacts with cytoplasmic dynein 1 intermediate chain (DYNC1I1 or DYNC1I2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452J5W3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035851"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040589.1">
        <property type="protein sequence ID" value="ENSGAGP00000035851.1"/>
        <property type="gene ID" value="ENSGAGG00000025438.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000029">
        <property type="entry name" value="peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000009">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6 14" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="15">
        <location>
            <begin position="13"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="14">
        <location>
            <begin position="301"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00077823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="364" mass="39771" checksum="08ECD43DAB9320FE" modified="2019-05-08" version="1">MAPPSPVANTRVFFEVDIGGERVGRIVLELFADVVPKTAENFRALCTGEKGIGPTTGKPLHYKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDQAGLLSMANAGPNTNGSQFFITTVPTPHLDGKHVVFGRVIKGMGVAKILENVEVIGEKPAQLCIIAECGELKEGDNWGISPMDGSGDTHPDFPEDSDVDLKDVDKIVSIAEDIKNIGNTFFKSQNWAMATKKYTKGLRYVEASKAIAEESDTAKLNPAALTCLLNIAACKLKVSDWQGAIESCCEALALDPANTKALYRRAQGWQGIRDFDQALADLKKAQEITPEDKAIQTEALKVKQKIKAQKEKEKAAYAKMFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J6X4</accession>
    <name>A0A452J6X4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J6X4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036241"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041028.1">
        <property type="protein sequence ID" value="ENSGAGP00000036241.1"/>
        <property type="gene ID" value="ENSGAGG00000025677.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="237"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="510" mass="58282" checksum="F7AA6B9373B696C0" modified="2019-05-08" version="1">MDFRKADFGKLRELIGKVPWELRLRGKTTEESWQFFKGTLLRAQKQVIPMVRKDRKCGKRPPWLTLEILHDLQNKKASYKKWKLGQITKDEYRQITQECRGKIRKAKAQNELKLAMGIKGNKKTFYQYIRSKRKTKDRVGPLLNEEGVTVTGDLEMAEMLNDFFVSVFTEKSEGMSSIVNAYGKRVGLEEKIRKEQVKNHLEKLDACKSPGPDEMHPRILKELIEEVSEPLAIIFGKSWETGEIPEDWKGANIVPIYKKGNKNNPGNYRPVSLTSVPGKIMEQVIKEIICKHLEGGKVIGNSQHGFVKNKSCQTNLIAFFDRITSLVDKGEAVDVIYLDFSKAFDTVSHDILIDKLGKYNLDGATIRWVHNWLDNRTQRVVVNGSQSCWKGITSGVPQGSVLGPVLFNIFINDLDVGIESTLIKFADDTKLGGIATALEDRVKIQNDLDKLEKWSEVNRMKFNKDKCKVLHLGRNNQFHTYRMGRDCLGRSMAERDLGVIVDHKLNMSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7B5</accession>
    <name>A0A452J7B5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rho GTPase-activating protein SYDE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Synapse defective protein 1 homolog 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase activator for the Rho-type GTPases. As a GCM1 downstream effector, it is involved in placental development and positively regulates trophoblast cells migration. It regulates cytoskeletal remodeling by controlling the activity of Rho GTPases including RHOA, CDC42 and RAC1.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J7B5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036388"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041209.1">
        <property type="protein sequence ID" value="ENSGAGP00000036388.1"/>
        <property type="gene ID" value="ENSGAGG00000025797.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097060">
        <property type="term" value="C:synaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000051">
        <property type="entry name" value="Synapse defective Rho GTPase homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052118">
        <property type="entry name" value="Rho-GAP_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057459">
        <property type="entry name" value="SYDE1/2_C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46150">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 100F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46150:SF2">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN SYDE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25336">
        <property type="entry name" value="C2_SYDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0564">Palmitate</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="10">
        <location>
            <begin position="506"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="11">
        <location>
            <begin position="659"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="120"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="269"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="370"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00074687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1012" mass="110655" checksum="488D525563DC3FB4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7C1</accession>
    <name>A0A452J7C1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J7C1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036259"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041047.1">
        <property type="protein sequence ID" value="ENSGAGP00000036259.1"/>
        <property type="gene ID" value="ENSGAGG00000025694.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001072">
        <property type="entry name" value="Immunoglobulin heavy variable V1-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="11" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="2"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="116" mass="12662" checksum="29AF55B5ECC75436" modified="2019-05-08" version="1">LPGGQSQVQLVQSGAEIRKPGESLKILCKTSGFTLTSYYVNWLQQAPGKGLVWVGRTDPANGATIYSQSFKDRFTITTDNSISTMYLQLGSLRADDTAVYYCARHTNCTSTSQDQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7C4</accession>
    <name>A0A452J7C4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase 4 regulatory subunit 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Putative regulatory subunit of serine/threonine-protein phosphatase 4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complex PPP4C-PPP4R4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J7C4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036402"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041226.1">
        <property type="protein sequence ID" value="ENSGAGP00000036402.1"/>
        <property type="gene ID" value="ENSGAGG00000025810.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008287">
        <property type="term" value="C:protein serine/threonine phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000097">
        <property type="entry name" value="Serine/threonine-protein phosphatase 4 regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039918">
        <property type="entry name" value="PPP4R4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21467">
        <property type="entry name" value="PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4 PPP4R4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21467:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="132"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="171"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="632"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="752"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="648"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="659"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="752"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="781"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="792" mass="90202" checksum="DA4F57EDBEDB55E5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7L0</accession>
    <name>A0A452J7L0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cysteine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="2">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tRNA(Cys) + L-cysteine + ATP = L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:17774"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452J7L0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036342"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041149.1">
        <property type="protein sequence ID" value="ENSGAGP00000036342.1"/>
        <property type="gene ID" value="ENSGAGG00000025758.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10310">
        <property type="entry name" value="GST_C_CysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA synthetases class I catalytic" evidence="16">
        <location>
            <begin position="124"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="738"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="234"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="738"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="830" mass="95827" checksum="43D8735BB5A07B11" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GEQ8</accession>
    <name>A0A452GEQ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AP-3 complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GEQ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000013"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000014.1">
        <property type="protein sequence ID" value="ENSGAGP00000000013.1"/>
        <property type="gene ID" value="ENSGAGG00000000007.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026740">
        <property type="entry name" value="AP3_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056314">
        <property type="entry name" value="AP3B1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029394">
        <property type="entry name" value="AP3B1_Ser"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029390">
        <property type="entry name" value="AP3B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015151">
        <property type="entry name" value="B-adaptin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14796">
        <property type="entry name" value="AP3B1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24080">
        <property type="entry name" value="AP3B1_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14797">
        <property type="entry name" value="SEEEED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037096">
        <property type="entry name" value="AP3_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01355">
        <property type="entry name" value="AP3B1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01020">
        <property type="entry name" value="B2-adapt-app_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP-3 complex subunit beta C-terminal" evidence="14">
        <location>
            <begin position="816"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-adaptin appendage C-terminal subdomain" evidence="13">
        <location>
            <begin position="987"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="673"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="681"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="700"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="729"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="764"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="778"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="804"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1099" mass="122180" checksum="F83D4C6E62C708B0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GF56</accession>
    <name>A0A452GF56_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GF56"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000178"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000207.1">
        <property type="protein sequence ID" value="ENSGAGP00000000178.1"/>
        <property type="gene ID" value="ENSGAGG00000000108.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF180">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="6">
        <location>
            <begin position="77"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="81"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="352" mass="38077" checksum="8849C080DC5B153F" modified="2019-05-08" version="1">GASDGRNSLGIRCSLTPCHSALGGIWAARGLQPTMASFSRSGVTLGPSSRLFSGNASIVGPGLGGGARERGLLSMNEKDTMQNLNDRLAAYLDMVRSLEEANGQLERQIREVCAKRALVGCQDLSGYFSTIAELRSQETAALRGHLGGSVSVEVDSAPGVDLVKTLAEIRDQYEDVIERNHREAAAWHKEQVGASQQQATSWSATQGARTKITELRRVVQGLEIELQSLHSMALEGTLAETQAGYGDKLTQLRGRGARKEAELVQLRTDAQRQAEEHQQLLDLKTRLEMEIATYRCLLEGDDVRDWLPLHPAGNGCSPRSPGRLGQCGGGFCSRCRPGENPGRDPGPIRGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFT5</accession>
    <name>A0A452GFT5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Target of rapamycin complex subunit lst8</fullName>
            <shortName evidence="11">TORC subunit lst8</shortName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Subunit of TORC1 and TORC2, which regulate cell growth and survival in response to nutrient and hormonal signals.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of TORC1 complex. Part of the TORC2 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the WD repeat LST8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GFT5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000436"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000490.1">
        <property type="protein sequence ID" value="ENSGAGP00000000436.1"/>
        <property type="gene ID" value="ENSGAGG00000000371.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000086">
        <property type="entry name" value="target of rapamycin complex subunit LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037588">
        <property type="entry name" value="MLST8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842">
        <property type="entry name" value="G BETA-LIKE PROTEIN GBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842:SF0">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="81"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="216"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="277"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="326" mass="36092" checksum="D75814320D2903B7" modified="2019-05-08" version="1">MNAGQGTVGSDPVILATAGYDHTVRFWQAHSGICTRTVQHQDSQVNALEITPDRSMIAAAGYQHIRMYDLNSNNPNPIINYDGVSKNITSVGFHEDGRWMYTGGEDCMARIWDLRSRNLQCQRIFQVNAPINCVCLHPNQAELIVGDQSGAIHIWDLKTDHNEQLIPEPEVSVNSVHIDPDASYMAAVNSSGNCYVWNLTGGIGEEVTQLIPKTKIPAHNRYALQCKFSPDSTLLATCSADQTCKIWRTSNFSLMTELSIKSNNPGETSRGWMWDCAFSGDSQYIVTASSDNLARLWCVETGEIKREYSGHQKAVVCLAFNDSVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GG86</accession>
    <name>A0A452GG86_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CYRIA/CYRIB Rac1 binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CYRI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GG86"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000590"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000663.1">
        <property type="protein sequence ID" value="ENSGAGP00000000590.1"/>
        <property type="gene ID" value="ENSGAGG00000000506.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039789">
        <property type="entry name" value="CYRI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009828">
        <property type="entry name" value="CYRIA/CYRIB_Rac1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12422">
        <property type="entry name" value="GH09096P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07159">
        <property type="entry name" value="CYRIA-B_Rac1-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYRIA/CYRIB Rac1 binding" evidence="5">
        <location>
            <begin position="72"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="376" mass="42819" checksum="616656088B480339" modified="2019-05-08" version="1">KKQKWWGEQSRFRVLTQTPRGRGTQAAQTAVLRTGACPGFTAAHLLLHLEVRSQVGRQRETPLYLQNRYQLYLAQADAQPTEAETAVWNQVNAVLEEAQTILAELQSYTGAGLEIREAIQNPNDLQLQEKAWNAVCPLVAKLKRFYEFSLRLENALRSLLEALTSPPYAPTQHLEREQALAKQFAEILHFTLTFDEFKMTNPAIQNDFSYYRRTLSRNRINNLQLDAESDVNNEMANRMSLFYAEATPMLKTLSNATTKFVSENKTLPIEDTTDCLSTMACVCRVMLETPEYRSRFTNTETLLFCMRVMVGVIILYDHVHPVGAFAKTSKIDMKGCIKVLKDQPSTSTEGLLNALRYTTRHLNDDTTSKQIRALLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHF6</accession>
    <name>A0A452GHF6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GHF6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001041"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001173.1">
        <property type="protein sequence ID" value="ENSGAGP00000001041.1"/>
        <property type="gene ID" value="ENSGAGG00000000843.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoxygenase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="132" mass="14690" checksum="1BCA96EBE104218E" modified="2025-10-08" version="2">MGVPGCSDCEDLSGLQYEFGAWMPNFPASMRNPPPQAKGTSSLGNYLDTIPEVNSTSNGLFALWVICCKPGDSRSLGTHPDEHFTEDEPKRLIKTFQGRLAQISQEIQERNKSLPIPYRYLDPLQIENSTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHN3</accession>
    <name>A0A452GHN3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GHN3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001129"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001264.1">
        <property type="protein sequence ID" value="ENSGAGP00000001129.1"/>
        <property type="gene ID" value="ENSGAGG00000000913.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048633">
        <property type="entry name" value="ITGAX-like_Ig_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF84">
        <property type="entry name" value="INTEGRIN ALPHA-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21520">
        <property type="entry name" value="ITGAX-like_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 16" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0401">Integrin</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018819912" description="VWFA domain-containing protein" evidence="16">
        <location>
            <begin position="29"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1097"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="157"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="445"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="508"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="570"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1136" mass="124880" checksum="3633706037EE2E97" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GID5</accession>
    <name>A0A452GID5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CUB and Sushi multiple domains 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GID5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001412"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001597.1">
        <property type="protein sequence ID" value="ENSGAGP00000001412.1"/>
        <property type="gene ID" value="ENSGAGG00000001131.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000001">
        <property type="entry name" value="CUB and sushi domain-containing protein 3 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF37">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="20"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="5">
        <location>
            <begin position="139"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="204"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="35049" checksum="2FB52641756DB34F" modified="2019-05-08" version="1">FTSFPKLFRWHLLMINKVFCRGKLCIHTFYVFQGPNGTIQSPGFPYGYPNYANCTWTVTAEEQNRIQLVFQSFALEEDFDVLSVYDGLPQQGNLRTRLTGFQLPAPIVSTGVVLSLWLVSDYAVSAQGFQANYEVLPSHTCGNPGRLQNGIQQGMTFSIGDKVRYSCNPGFFLEGHALLTCHASSENSASWDFPLPSCRADDACGGTLRGQSGIISSPHYPSEYGNNADCTWTILAEPGDTIALVFMDFQLEDGYDFLEVTGTEGSSLWFTGMNLPAPVISSKNWLRLHFTSDGNHRQKGFSAQYQGNCLLTILNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJ83</accession>
    <name>A0A452GJ83_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein 196</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GJ83"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001672"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001900.1">
        <property type="protein sequence ID" value="ENSGAGP00000001672.1"/>
        <property type="gene ID" value="ENSGAGG00000001339.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037661">
        <property type="entry name" value="TMEM196"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28681">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28681:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="185" mass="19906" checksum="8D4D3B3C3A90E48F" modified="2019-05-08" version="1">MCTSSHTIGSLLVLSVLEIGLGVSSVAVGAVSFSLVLAEHKPQLGDSSPVWSGVCFLLCGICGILCAKKKSGLVMILFSACCICGLIGGILNFQFLRALTKKSSTLYSLHLTSMSLACIGIGGCTLSSWLTCRLASYEQRRMFSEREHSLHHSHEMAEKRLRGIEIIDLPSCPVIPPTPELPQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJ95</accession>
    <name>A0A452GJ95_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Spermatogenesis-associated protein 16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Acrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the SPATA16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GJ95"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001682"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001912.1">
        <property type="protein sequence ID" value="ENSGAGP00000001682.1"/>
        <property type="gene ID" value="ENSGAGG00000001348.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000281">
        <property type="entry name" value="Spermatogenesis associated 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029161">
        <property type="entry name" value="SPATA16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47228">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47228:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15015">
        <property type="entry name" value="NYD-SP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0744">Spermatogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="356" mass="40049" checksum="1B9981F1F06A04BE" modified="2019-05-08" version="1">MESASNTGLGNIINKVCQNETSPKANASRERTVAANKLNDIPEALQEVKKHPWETAVENVMERIKMAKNGKEKQSHDLDRAGFKRKLESEEKPAVKKEARMLELDNQLATMPLPHIPLKSLMDVEIKLVYIDEEDITYEFVGSMASTSTQSTCRDVEIVDSLSAPNFSFLPQIDKWLQVALKDASTCYRQKKYAVAAGQFRTALELCSKGAALGKPFDASPQDISSIASFIETKLVTCYLRMRKPDLALNHSHRSIILNPAYFLNHLRQAAVFRWLERYSEAARSAMIADYMYWLSGGSEQHISKLIKLYWQPLSQTHPCVHTHTHTHIYALETLLTPVTACESLLLPVSLFLGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GL88</accession>
    <name>A0A452GL88_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein mS38</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mitochondrion-specific ribosomal protein mS38 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GL88"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002369"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002718.1">
        <property type="protein sequence ID" value="ENSGAGP00000002369.1"/>
        <property type="gene ID" value="ENSGAGG00000001927.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013177">
        <property type="entry name" value="Ribosomal_mS38_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32035">
        <property type="entry name" value="AURORA KINASE A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32035:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08213">
        <property type="entry name" value="COX24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01155">
        <property type="entry name" value="DUF1713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein mS38 C-terminal" evidence="5">
        <location>
            <begin position="128"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="201" mass="23647" checksum="76329C06726F4E15" modified="2019-05-08" version="1">MLISRLTSQLAKTSRFAGHFLPRSVSSVLCSGPTPANYSTQPSDKNGAQPQHWYALDPELEEMLVPRKMSISPLESWLTVRYSLPKVEIINVHEKLGYEPTQQYDCPPCEREADVEEEEGEVSTNKVECKNVLKIRRRKMNRHKYKKLLKRTRFLRRKILDGRRKKRQAKFERDLKRIWRKAGLKKPSEGWQLPKIFVRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLR8</accession>
    <name>A0A452GLR8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Methionine synthase reductase</fullName>
            <ecNumber evidence="14">1.16.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Aquacobalamin reductase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2 cob(II)alamin + A + 2 H2O + 2 H(+) = 2 aquacob(III)alamin + AH2</text>
            <dbReference type="Rhea" id="RHEA:20752"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15852"/>
            <dbReference type="ChEBI" id="CHEBI:16304"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:20754"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Forms a multiprotein complex with MMACHC, MMADHC and MTR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.16.1.8" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A452GLR8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002618"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002992.1">
        <property type="protein sequence ID" value="ENSGAGP00000002618.1"/>
        <property type="gene ID" value="ENSGAGG00000002099.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030586">
        <property type="term" value="F:[methionine synthase] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009235">
        <property type="term" value="P:cobalamin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046655">
        <property type="term" value="P:folic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050667">
        <property type="term" value="P:homocysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06203">
        <property type="entry name" value="methionine_synthase_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000007">
        <property type="entry name" value="Methionine synthase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000025">
        <property type="entry name" value="methionine synthase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000018">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF84">
        <property type="entry name" value="METHIONINE SYNTHASE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="13" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="19">
        <location>
            <begin position="9"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="20">
        <location>
            <begin position="277"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00062273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00075467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="704" mass="78950" checksum="510E91FDAA5943B0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLX7</accession>
    <name>A0A452GLX7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nipped-B protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000002812"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003216.1">
        <property type="protein sequence ID" value="ENSGAGP00000002812.1"/>
        <property type="gene ID" value="ENSGAGG00000002239.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048565">
        <property type="term" value="P:digestive tract development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048703">
        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23958">
        <property type="entry name" value="SCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000225">
        <property type="entry name" value="Nipped-B protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026003">
        <property type="entry name" value="Cohesin_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024986">
        <property type="entry name" value="Nipped-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12765">
        <property type="entry name" value="Cohesin_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12830">
        <property type="entry name" value="Nipped-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="9 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sister chromatid cohesion C-terminal" evidence="12">
        <location>
            <begin position="2282"/>
            <end position="2462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="131"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="269"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="484"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="992"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1033"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1696"/>
            <end position="1720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2479"/>
            <end position="2535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2667"/>
            <end position="2711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="131"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="269"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="484"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="564"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="593"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="613"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="631"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="690"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="933"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1062"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1083"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1145"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1696"/>
            <end position="1718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="2479"/>
            <end position="2491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="2492"/>
            <end position="2511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2819" mass="318500" checksum="58EE6BF93EAF1990" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GML8</accession>
    <name>A0A452GML8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TAFH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GML8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003070"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003528.1">
        <property type="protein sequence ID" value="ENSGAGP00000003070.1"/>
        <property type="gene ID" value="ENSGAGG00000002411.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08045">
        <property type="entry name" value="HFD_TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000015">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1110:FF:000002">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1110">
        <property type="entry name" value="TAFH/NHR1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045144">
        <property type="entry name" value="TAF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007900">
        <property type="entry name" value="TAF4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037249">
        <property type="entry name" value="TAFH/NHR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003894">
        <property type="entry name" value="TAFH_NHR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15138">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15138:SF17">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05236">
        <property type="entry name" value="TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07531">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00549">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158553">
        <property type="entry name" value="TAFH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51119">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFH" evidence="8">
        <location>
            <begin position="204"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="643"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="774" mass="83500" checksum="64AF9D200FBE2963" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNC8</accession>
    <name>A0A452GNC8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sister chromatid cohesion protein DCC1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GNC8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003362"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003874.1">
        <property type="protein sequence ID" value="ENSGAGP00000003362.1"/>
        <property type="gene ID" value="ENSGAGG00000002716.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034088">
        <property type="term" value="P:maintenance of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019128">
        <property type="entry name" value="Dcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395:SF6">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09724">
        <property type="entry name" value="Dcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="389" mass="44704" checksum="D73697B32E02DFCC" modified="2019-05-08" version="1">MRSREEVEATLRIAKLNPAELLPPVHCLRFNPRASAGECCLLQLEPGLCAELEAGHSLVIRGEKDEQAVLCSKDKTYDMKIADTSNMLLFIPGCKTPEQLLADQASCNIIHTQIAGFSNNYWELRKCRPKLKKLKKLLMENPYEGPDSEREKALTDSKYTMEDLLDRIQASEEEILHQLQVINACEIEGYWRVLEFDYEMKLLNHVTQLIDSESWSFSKVPLSICLQELGPLEPEEMIQHILLSYGMKYTDEDEVYFEMKADKICTATAQMLLQNAVKFNLAEFQEVWQQSVPEGLTTRLDQLKGLALLDRNSRPETIFLLKVEELPEDNQERFNKLFTIREKWTEADIAPYIQDLCAEKQTVAALLTKYARSSMQNGVKVYNSRRPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNY7</accession>
    <name>A0A452GNY7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="45">Autotaxin</fullName>
            <ecNumber evidence="44">3.1.4.39</ecNumber>
            <ecNumber evidence="5">3.1.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="46">Ectonucleotide pyrophosphatase/phosphodiesterase family member 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="47">Extracellular lysophospholipase D</fullName>
        </alternativeName>
    </protein>
    <organism evidence="50 51">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="51" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="50" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>1,2-didecanoyl-sn-glycero-3-phosphocholine + H2O = 1,2-didecanoyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41412"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:78226"/>
            <dbReference type="ChEBI" id="CHEBI:78227"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:41413"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O = 1,2-dioctanoyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41416"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:78228"/>
            <dbReference type="ChEBI" id="CHEBI:78229"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:41417"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41139"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74344"/>
            <dbReference type="ChEBI" id="CHEBI:74938"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:41140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>1-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphocholine + H2O = 1-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41135"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28733"/>
            <dbReference type="ChEBI" id="CHEBI:74547"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:41136"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + H2O = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + L-serine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38931"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74617"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:38932"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38915"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28610"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:38916"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ethanolamine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38927"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74971"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:38928"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>1-O-(9Z-octadecenyl)-sn-glycero-3-phosphocholine + H2O = 1-O-(9Z-octadecenyl)-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41684"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64396"/>
            <dbReference type="ChEBI" id="CHEBI:78402"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:41685"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1-O-hexadecyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41143"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
            <dbReference type="ChEBI" id="CHEBI:77580"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:41144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-decanoyl-sn-glycero-3-phosphocholine + H2O = 1-decanoyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41131"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:77724"/>
            <dbReference type="ChEBI" id="CHEBI:77726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:41132"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1-dodecanoyl-sn-glycero-3-phosphocholine + H2O = 1-dodecanoyl-sn-glycerol 3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38991"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72682"/>
            <dbReference type="ChEBI" id="CHEBI:74966"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:38992"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38975"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57518"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:38976"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-hexanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexanoyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41400"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:78215"/>
            <dbReference type="ChEBI" id="CHEBI:78223"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:41401"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>1-octadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-octadecanoyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38979"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:74565"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:38980"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>1-tetradecanoyl-sn-glycero-3-phosphocholine + H2O = 1-tetradecanoyl-sn-glycerol 3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38983"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64489"/>
            <dbReference type="ChEBI" id="CHEBI:72683"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:38984"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:14446"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine + H2O = a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41588"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:77283"/>
            <dbReference type="ChEBI" id="CHEBI:77287"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:41589"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 1-O-alkyl-sn-glycero-3-phosphocholine + H2O = a 1-O-alkyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39927"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30909"/>
            <dbReference type="ChEBI" id="CHEBI:58014"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:39928"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 1-O-alkyl-sn-glycero-3-phosphoethanolamine + H2O = a 1-O-alkyl-sn-glycero-3-phosphate + ethanolamine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:58014"/>
            <dbReference type="ChEBI" id="CHEBI:76168"/>
            <dbReference type="EC" id="3.1.4.39"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>a 1-acyl-sn-glycero-3-phospho-L-serine + H2O = a 1-acyl-sn-glycero-3-phosphate + L-serine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38999"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:64379"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:39000"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38995"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:38996"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>a 1-acyl-sn-glycero-3-phosphoethanolamine + H2O = a 1-acyl-sn-glycero-3-phosphate + ethanolamine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39003"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:64381"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:39004"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>a 2-acyl-sn-glycero-3-phospho-L-serine + H2O = a 2-acyl-sn-glycerol 3-phosphate + L-serine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41716"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:64982"/>
            <dbReference type="ChEBI" id="CHEBI:65214"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:41717"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>a 2-acyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycerol 3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41712"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57875"/>
            <dbReference type="ChEBI" id="CHEBI:64982"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:41713"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>sphing-4-enine-phosphocholine + H2O = sphing-4-enine 1-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38919"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58906"/>
            <dbReference type="ChEBI" id="CHEBI:60119"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:38920"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.39" evidence="44"/>
    <dbReference type="EC" id="3.1.4.4" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452GNY7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003462"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003992.1">
        <property type="protein sequence ID" value="ENSGAGP00000003462.1"/>
        <property type="gene ID" value="ENSGAGG00000002768.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000004007.1">
        <property type="protein sequence ID" value="ENSGAGP00000003474.1"/>
        <property type="gene ID" value="ENSGAGG00000002768.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047391">
        <property type="term" value="F:alkylglycerophosphoethanolamine phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004528">
        <property type="term" value="F:phosphodiesterase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034638">
        <property type="term" value="P:phosphatidylcholine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00091">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.570.10:FF:000001">
        <property type="entry name" value="Ectonucleotide pyrophosphatase/phosphodiesterase family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000006">
        <property type="entry name" value="Ectonucleotide pyrophosphatase/phosphodiesterase family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.20:FF:000001">
        <property type="entry name" value="Ectonucleotide pyrophosphatase/phosphodiesterase family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.20:FF:000002">
        <property type="entry name" value="Ectonucleotide pyrophosphatase/phosphodiesterase family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020436">
        <property type="entry name" value="SMB_chordata"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036024">
        <property type="entry name" value="Somatomedin_B-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF21">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01033">
        <property type="entry name" value="Somatomedin_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00022">
        <property type="entry name" value="SOMATOMEDINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00201">
        <property type="entry name" value="SO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90188">
        <property type="entry name" value="Somatomedin B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00524">
        <property type="entry name" value="SMB_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="8" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="48" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="51" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="48" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="48" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="48">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="49">
        <location>
            <begin position="55"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="49">
        <location>
            <begin position="99"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00050165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00050281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00050325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00050335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00050409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00050678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00050734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00050999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00051129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00051145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00051174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00051204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00051248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00051348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00051367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00051368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00051488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00051825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00051847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00052276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00052301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00052470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="ARBA" id="ARBA00052908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
            <dbReference type="ARBA" id="ARBA00066852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="45">
        <source>
            <dbReference type="ARBA" id="ARBA00071948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="46">
        <source>
            <dbReference type="ARBA" id="ARBA00075924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="47">
        <source>
            <dbReference type="ARBA" id="ARBA00081337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="48">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="49">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="50">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="51">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="858" mass="98263" checksum="BE93AF217480D966" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GP47</accession>
    <name>A0A452GP47_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GP47"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003664"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004233.1">
        <property type="protein sequence ID" value="ENSGAGP00000003664.1"/>
        <property type="gene ID" value="ENSGAGG00000002971.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06603">
        <property type="entry name" value="GH31_GANC_GANAB_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000046">
        <property type="entry name" value="Glucosidase alpha, neutral C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000039">
        <property type="entry name" value="Glucosidase, alpha neutral C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000023">
        <property type="entry name" value="neutral alpha-glucosidase AB isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF60">
        <property type="entry name" value="NEUTRAL ALPHA-GLUCOSIDASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="7">
        <location>
            <begin position="72"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="6">
        <location>
            <begin position="358"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="8">
        <location>
            <begin position="694"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="919" mass="105467" checksum="9C926198AFEF9DB4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPX2</accession>
    <name>A0A452GPX2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPX2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003949"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004596.1">
        <property type="protein sequence ID" value="ENSGAGP00000003949.1"/>
        <property type="gene ID" value="ENSGAGG00000003231.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07861">
        <property type="entry name" value="STKc_CDK1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000231">
        <property type="entry name" value="Cyclin-dependent kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000027">
        <property type="entry name" value="Putative Cyclin-dependent kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF334">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="4"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="303" mass="34667" checksum="523D5E14A184C9DA" modified="2019-05-08" version="1">MDDYTKIEKIGEGTYGVVYKGRHKSTGQVVAMKKIRLESEEEGVPSTAIREISLLKELHHPNIVCLQDVLMQDSRLYLIFEFLSMDLKKYLDSVPSGQFVDSMLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGVIKLADFGLARAFGIPVRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAEMATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDEDGLDLLSKMLIYDPAKRISGKMALNHPYFDDLDKSNLPANQIKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPX5</accession>
    <name>A0A452GPX5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">BTB/POZ domain-containing protein TNFAIP1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the BACURD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPX5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003881"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004505.1">
        <property type="protein sequence ID" value="ENSGAGP00000003881.1"/>
        <property type="gene ID" value="ENSGAGG00000003175.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035024">
        <property type="term" value="P:negative regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18401">
        <property type="entry name" value="BTB_POZ_TNFAIP1_BACURD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000013">
        <property type="entry name" value="BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF17">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="12">
        <location>
            <begin position="31"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="313" mass="35961" checksum="5048479ACED8984A" modified="2019-05-08" version="1">MSGDTCLTTICQSSGAKPKTCSFREGSLGNKYVRLNVGGSLYYTTVQVLTRHDTMLKAMFSGRMEVLTDKEGWILIDRCGKHFGVILNYLRDDTIALPKNRQEIKELMAEAKYYLIQGLVDMCQAALQDKKDSYEPVCSIPIITSPKEEERLIESSMKPVVKLLYNRSNNKYSYTSNSDDNLLKNIELFDKLSLRFNGRVLFLKDVIGDEICCWSFYGQGRKLAEVCCTSIVYATEKKQTKVEFPEARIYEETLNVLLYETPRVPDNSLLEATSRNRSQASHSEDDEGFELRDRVRRIHVKRYSTYDDRQLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQ17</accession>
    <name>A0A452GQ17_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQ17"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004003"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004662.1">
        <property type="protein sequence ID" value="ENSGAGP00000004003.1"/>
        <property type="gene ID" value="ENSGAGG00000003276.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000812">
        <property type="entry name" value="zinc finger protein 23 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001437">
        <property type="entry name" value="Zinc finger protein 594"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000009">
        <property type="entry name" value="zinc finger protein 79 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050826">
        <property type="entry name" value="Krueppel_C2H2_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24377">
        <property type="entry name" value="IP01015P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="239"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="286" mass="33561" checksum="A0D0FD718831D513" modified="2019-05-08" version="1">MRESTQGRDPMNASLSTSENPHRGKPYECCECGKCFTQGSALIRHQRIHTGDRPYECRECRKTFSRSSHLIGHQRIHTGERSYECSECGKTFNHSSNLIRHHRIHTGERPYECRECGKTFTQRSGLFHHQKIHTGVRTISALSKDWRIHTGERSYECRKCGKTFSRSSHLIWHQRIHTRERPYECRECGKCFTSSSDLFVHHRIHTGERPYECCECGKCVTHGSALIRHQRIHTGERPYECRDECGKTFNHSSNLIRHHRIHTGERPYECRGWLISPCFKIQGVWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQC9</accession>
    <name>A0A452GQC9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GQC9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004144"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004846.1">
        <property type="protein sequence ID" value="ENSGAGP00000004144.1"/>
        <property type="gene ID" value="ENSGAGG00000003417.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000021019.1">
        <property type="protein sequence ID" value="ENSGAGP00000018444.1"/>
        <property type="gene ID" value="ENSGAGG00000013653.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604451" evidence="1">
        <location>
            <begin position="22"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="92" mass="10859" checksum="C2B9BC1E74A6FD1F" modified="2019-05-08" version="1">MRTCYSLSFLFWTCMLHVVHMTPSPKRTIKYSQNGRILVLSQDDGKTLHRSKRGWMWNQFFLLEEYTGSDTQYVGKVSFAFKKPQTVDKSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQR2</accession>
    <name>A0A452GQR2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Terminal nucleotidyltransferase 4B</fullName>
            <ecNumber evidence="6">2.7.7.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Non-canonical poly(A) RNA polymerase PAPD5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">PAP-associated domain-containing protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">Terminal guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Terminal uridylyltransferase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Topoisomerase-related function protein 4-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452GQR2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004291"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005038.1">
        <property type="protein sequence ID" value="ENSGAGP00000004291.1"/>
        <property type="gene ID" value="ENSGAGG00000003561.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070568">
        <property type="term" value="F:guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060212">
        <property type="term" value="P:negative regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905870">
        <property type="term" value="P:positive regulation of 3'-UTR-mediated mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000006">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000015">
        <property type="entry name" value="Terminal nucleotidyltransferase 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF51">
        <property type="entry name" value="TERMINAL NUCLEOTIDYLTRANSFERASE 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="23" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="11" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="21" id="KW-0464">Manganese</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="20" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="33">
        <location>
            <begin position="169"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="32">
        <location>
            <begin position="357"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="41"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="518"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="31">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="31">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="31">
        <location>
            <begin position="527"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="31">
        <location>
            <begin position="562"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="31">
        <location>
            <begin position="587"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="31">
        <location>
            <begin position="599"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00067210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00076411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00076530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00079354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00081892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00082009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="669" mass="74064" checksum="2DBDC9B5C7A7ECED" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GT35</accession>
    <name>A0A452GT35_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GT35"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005167"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006026.1">
        <property type="protein sequence ID" value="ENSGAGP00000005167.1"/>
        <property type="gene ID" value="ENSGAGG00000004203.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021884">
        <property type="term" value="P:forebrain neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09381">
        <property type="entry name" value="LIM1_Lhx7_Lhx8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09383">
        <property type="entry name" value="LIM2_Lhx7_Lhx8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000050">
        <property type="entry name" value="LIM homeobox 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000023">
        <property type="entry name" value="LIM homeobox 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000031">
        <property type="entry name" value="LIM homeobox 6, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF117">
        <property type="entry name" value="LIM_HOMEOBOX PROTEIN LHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="124"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="185"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="274"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="276"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="44"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="407" mass="44447" checksum="CA6020709E29E91C" modified="2019-05-08" version="1">MYWKSDQMFVCKLEDKDIPALGISTEKFPGLMAEECGRAAALAGGRRRKGAGEGALVSEGGAGAGGEGAGGAGRSERANRPPALPQVSPEAAGDEDSCSSSTPLSPSSSPRSMASASGCAPSKCLCSSCGLEIVDKYLLKVNDLCWHVRCLSCSVCRTSLGRHTSCYIKDKDIFCKLDYFRRYGTRCSRCGRHIHSTDWVRRAKGNVYHLACFACFSCKRQLSTGEEFALVEEKVLCRVHYDCMLDNLKREVENGNGISMEGALLTEQDVNHPKPAKRARTSFTADQLQVMQAQFAQDNNPDAQTLQKLAERTGLSRRVIQVWFQNCRARHKKHVSPNHSSTAPVTAVQPSRLSPPMLEEMAYSAYVPQDGTMLTALHTYMDAHSPTALGLQPLLPHSMTQLPISHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTH8</accession>
    <name>A0A452GTH8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTH8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005346"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006221.1">
        <property type="protein sequence ID" value="ENSGAGP00000005346.1"/>
        <property type="gene ID" value="ENSGAGG00000004319.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015129">
        <property type="term" value="F:lactate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF15">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="182"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="269"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="329"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="376"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="500"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="586"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="600"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="611" mass="66728" checksum="ED642905369F2904" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTS0</accession>
    <name>A0A452GTS0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome b-c1 complex subunit 2, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Complex III subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Core protein II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquinol-cytochrome-c reductase complex core protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="15">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)). Interacts with RAB5IF. Interacts with STMP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTS0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005120"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005968.1">
        <property type="protein sequence ID" value="ENSGAGP00000005120.1"/>
        <property type="gene ID" value="ENSGAGG00000004163.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000018">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000026">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF226">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="16">
        <location>
            <begin position="53"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="17">
        <location>
            <begin position="203"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="457" mass="48667" checksum="6B07DDCE65C67D19" modified="2019-05-08" version="1">MKGIPTIARSLSKRFYSLSVAPKVEASATAERGKLRLQPQDLEITRLPNGLVIASLENYSPASRIGVFIKAGSRYETTNNLGTSHLLRLASNLTTKGASSFKITRGIEAVGGNLSVTSTRENMIYSVECLRDYVDTVMEYLINVTTAPEFRPWEVTELQAQLKVDKAVAFQNPQVSVLENLHAAAYRNALANSLYCPDYAVGKITPEQLHQFVQNNFTSARMALVGLGVSHSELKQVGEQFLNIRSGAGSSGVKAQYRGGEIREQNGESLVHAAIVAEGVATGSAEANAFSVLQHILGAGPLIKRGSNISSKLFQAIAKATSQPFDAAAFNVNYSDSGLFGIYTISQASAAGEVIKAALNQVKAIAQGSITDEDVTRAKNHLKATFLMLVETSDGLLDEIGSEALASGTYTSPTAVIQNIDSIATADIVNAAKKFVDGKKSMAASGNLGNTPFVDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUF7</accession>
    <name>A0A452GUF7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GUF7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005397"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006283.1">
        <property type="protein sequence ID" value="ENSGAGP00000005397.1"/>
        <property type="gene ID" value="ENSGAGG00000004379.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="180" mass="17737" checksum="2DBEB652BD76BC4B" modified="2019-05-08" version="1">PTPSRFFHTSLSCPLTLGAPRPTAPSCPLTPGAPRPTAPSCPLTPGAPRPTAPSCPLTPGAPRPTAPSCPLTPGAPRPTAPSCPLTPGAPRPTAPSCPLTPGAPRPTAPSCPLTPGAPRPTAPSCPLTPGAPRPTAPSCLPGALRPATPGCPLTPGALHPTAPNCPLTPEHHVQQPLAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GV20</accession>
    <name>A0A452GV20_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Headcase middle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GV20"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005601"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006528.1">
        <property type="protein sequence ID" value="ENSGAGP00000005601.1"/>
        <property type="gene ID" value="ENSGAGG00000004547.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026066">
        <property type="entry name" value="Headcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031947">
        <property type="entry name" value="Headcase_mid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13425">
        <property type="entry name" value="HEADCASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13425:SF3">
        <property type="entry name" value="HEADCASE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16002">
        <property type="entry name" value="Headcase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Headcase middle" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="156" mass="17584" checksum="065E678DD87C7715" modified="2019-05-08" version="1">MEDDAQVGQGEDLRKFILAALSASHRNVVNCALCHRALPVFEQFPLVDGTLFLSPSRHDEIEYDVPCHLQGRLMHLYAVCVDCLEGVHKIICIKCKSRWDGSWHQLGTMYTYDILAASPCCQVSIMHNKVRLQQSRSLCLFSLLAPAFLKSPSLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXU0</accession>
    <name>A0A452GXU0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel HCN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXU0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006857"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007956.1">
        <property type="protein sequence ID" value="ENSGAGP00000006857.1"/>
        <property type="gene ID" value="ENSGAGG00000005530.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000031">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000002">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000007">
        <property type="entry name" value="Putative potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013621">
        <property type="entry name" value="Ion_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF11">
        <property type="entry name" value="POTASSIUM_SODIUM HYPERPOLARIZATION-ACTIVATED CYCLIC NUCLEOTIDE-GATED CHANNEL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08412">
        <property type="entry name" value="Ion_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0114">cAMP</keyword>
    <keyword evidence="7" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="19" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="16 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="4" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="8 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="25">
        <location>
            <begin position="382"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="619"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="619"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="657"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="684"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="725"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="760"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="803" mass="90228" checksum="0692D41853EF1B45" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GY33</accession>
    <name>A0A452GY33_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000006962"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008069.1">
        <property type="protein sequence ID" value="ENSGAGP00000006962.1"/>
        <property type="gene ID" value="ENSGAGG00000005610.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024854">
        <property type="entry name" value="Kinectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007794">
        <property type="entry name" value="Rib_rcpt_KP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18864">
        <property type="entry name" value="KINECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18864:SF1">
        <property type="entry name" value="KINECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05104">
        <property type="entry name" value="Rib_recp_KP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="9">
        <location>
            <begin position="744"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="48"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="307"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="407"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="566"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1116"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1373" mass="157133" checksum="A99EC68284997B9C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYM9</accession>
    <name>A0A452GYM9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endoplasmic reticulum resident protein 44</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Thioredoxin domain-containing protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protein folding in the endoplasmic reticulum. Required to retain ERO1A and ERO1B in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Forms mixed disulfides with both ERO1A and ERO1B and cargo folding intermediates; the interactions with ERO1A and ERO1B result in their retention in the endoplasmic reticulum. Directly interacts with ITPR1 in a pH-, redox state- and calcium-dependent manner, but not with ITPR2 or ITPR3. The strength of this interaction inversely correlates with calcium concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GYM9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007161"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008286.1">
        <property type="protein sequence ID" value="ENSGAGP00000007161.1"/>
        <property type="gene ID" value="ENSGAGG00000005755.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02996">
        <property type="entry name" value="PDI_a_ERp44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03072">
        <property type="entry name" value="PDI_b'_ERp44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03070">
        <property type="entry name" value="PDI_b_ERp44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000051">
        <property type="entry name" value="endoplasmic reticulum resident protein 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000074">
        <property type="entry name" value="endoplasmic reticulum resident protein 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000128">
        <property type="entry name" value="endoplasmic reticulum resident protein 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052643">
        <property type="entry name" value="ERP44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041870">
        <property type="entry name" value="ERp44_PDI_b_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041862">
        <property type="entry name" value="ERp44_PDI_b_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46295">
        <property type="entry name" value="ENDOPLASMIC RETICULUM RESIDENT PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46295:SF1">
        <property type="entry name" value="ENDOPLASMIC RETICULUM RESIDENT PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019574112" description="Endoplasmic reticulum resident protein 44" evidence="11">
        <location>
            <begin position="30"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="12">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="361"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="378"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="406" mass="46724" checksum="7C139BDB0D46F936" modified="2019-05-08" version="1">MRPAIFLPPPDPGCVLLLLVSWLLNPARSEITSLDSGNIDDILNNADVALVNFYADWCRFSQMLHPIFEEASNVIKEEYPDNNQVVFARVDCDQHSDIAQRYRISKYPTLKLFRNGMMMKREYRGQRSVTAIADYIRQQKSNPIREVNDLEEIKSLDRSKRNIIGYFEQKDTNNYKTFERVANILHDDCVFLSAFGPVSKPERFSGDNIIYKPPGENAPDMVYLGSMTNFDLTYAWTQDKCVPLVREITFENGEELTEEGLPFLILFHMKDDLESLEKFQQEVARQLISEKGTINFLHADCDKFRHPLLHIQKTPADCPVIAIDSFRHMYVFPDFSDLAIPGKLKQFVLDLHSGKLHREFHHGPDPTDVAPGQPIQDLASSPPESSFQKLAPSEHRYTLLREKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZI4</accession>
    <name>A0A452GZI4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Calcium-activated potassium channel subunit beta-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">BK channel subunit beta-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Calcium-activated potassium channel, subfamily M subunit beta-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Charybdotoxin receptor subunit beta-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">K(VCA)beta-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Maxi K channel subunit beta-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Slo-beta-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB2 per KCNMA1 tetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZI4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008636.1">
        <property type="protein sequence ID" value="ENSGAGP00000007478.1"/>
        <property type="gene ID" value="ENSGAGG00000005986.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015269">
        <property type="term" value="F:calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005513">
        <property type="term" value="P:detection of calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.81.20:FF:000001">
        <property type="entry name" value="Calcium-activated potassium channel beta 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.81.20">
        <property type="entry name" value="KCNMB2, ball/chain domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003930">
        <property type="entry name" value="K_chnl_Ca-activ_BK_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037096">
        <property type="entry name" value="KCNMB2_ball/chain_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015382">
        <property type="entry name" value="KCNMB2_ball_chain_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10258">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10258:SF5">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03185">
        <property type="entry name" value="CaKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09303">
        <property type="entry name" value="KcnmB2_inactiv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01450">
        <property type="entry name" value="BKCHANNELB"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="KCNMB2 ball/chain" evidence="19">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00084024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF09303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="235" mass="27223" checksum="A5EFEB7C435F435F" modified="2019-05-08" version="1">MFIWTSGRTSTSYRHDEKRNIYQKIRDHDLLDKRKTVTALKAGEDRAILLGLTMMVCSIMMYFLLGITLLRSYMQSVWTEEAQCTLLNASITEAFNCSFSCGPDCWKISQYPCLQVYVNLTSSGQKLLLYHTEETIKINSECSYIPKCGKNYEESMSLVSVVMENFRKYQRFSCFYDPEGIQKNVILTKLYSSNVLFHSLFWPTCMMIGGVAIVAMVKLTQYLSLLCERIQRINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0X4</accession>
    <name>A0A452H0X4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cation-transporting P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000008010"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009229.1">
        <property type="protein sequence ID" value="ENSGAGP00000008010.1"/>
        <property type="gene ID" value="ENSGAGG00000006371.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000065">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="195" mass="21994" checksum="3B5EFE01AA931AE2" modified="2019-05-08" version="1">MDKPPRSPKEPLISGWLFFRYMAIGGYVGAATVGAAAWWFLYAEDGPGVSYHQLSHFMQCTEENVDFEGLDCEVFESPVPMTMALSVLVTXLALLSENQSLVRMPPWVNIWLLSSICLSMSLHFVILYIDPLPMIFKLTPLELTHWLMVLKISFPVILLDEVLKFIARDYLEGKAASLPWSRPGYPRASQQDSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2I8</accession>
    <name>A0A452H2I8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MCRIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H2I8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008729"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010025.1">
        <property type="protein sequence ID" value="ENSGAGP00000008729.1"/>
        <property type="gene ID" value="ENSGAGG00000006880.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029428">
        <property type="entry name" value="MCRIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14799">
        <property type="entry name" value="FAM195"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="159" mass="17790" checksum="756C1053646A8D99" modified="2019-05-08" version="1">PATLVPCPSLCLDSQSQSPATKSTSSHYTMPPAPYPTACWLWMQTDMSFQPLILSLYLSSPTPKLVFNRVNGKRPLVTTQPTAATEESYTLAHEENVRFVYETWQQVEQQLDGSQPGESGRGPVQYAEKTPNPVLTNFVPIDLEEWWAQQFLAKIENGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H332</accession>
    <name>A0A452H332_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Hepatic and glial cell adhesion molecule</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Hepatocyte cell adhesion molecule</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation. In glia, associates and targets CLCN2 at astrocytic processes and myelinated fiber tracts where it may regulate transcellular chloride flux involved in neuron excitability.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer. Dimer formation occurs predominantly through cis interactions on the cell surface. Part of a complex containing MLC1, TRPV4, AQP4 and ATP1B1. Interacts with CLCN2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cell membrane</location>
            <topology evidence="17">Single-pass type I membrane protein</topology>
            <orientation evidence="17">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H332"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009019"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010355.1">
        <property type="protein sequence ID" value="ENSGAGP00000009019.1"/>
        <property type="gene ID" value="ENSGAGG00000007124.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000922">
        <property type="entry name" value="hepatocyte cell adhesion molecule isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000786">
        <property type="entry name" value="hepatocyte cell adhesion molecule isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052280">
        <property type="entry name" value="HEPACAM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44888">
        <property type="entry name" value="HEPACAM FAMILY MEMBER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44888:SF2">
        <property type="entry name" value="HEPATIC AND GLIAL CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0338">Growth arrest</keyword>
    <keyword evidence="5" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 23" id="KW-0732">Signal</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019289245" description="Hepatic and glial cell adhesion molecule" evidence="23">
        <location>
            <begin position="34"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="148"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="315"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00056251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00062787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00069546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="414" mass="46007" checksum="FA87779E3FF9A36D" modified="2019-05-08" version="1">MKREREALSRVPSAPGFVHLVCLLLIHPGLLEGVNITSPVLLIHGTVGKPALLSVRYTSTSSDKPVVKWQLKRDKPTTVVQSIGTEIIGNLRPDYKDRIKILENGSLLISHLQLSDEGTYEVEISITDDTFTGEKNINLTVDIPISKPHVVLASSTVLELSEYFTLNCSHENGTKPTYTWLKDGKPLSNDSRLLLSHDQKVLTITRVLMSDDDIYSCLVENPISRGRSVPIKLTVYRRSSLYIILSTGGIFLLVTLVTVCACWKPSKKDKQKLDKQSSSEYVDQNEEHLKHEVEIVPRSGEHECRNPVALYILKDKDSPEAEEESFQQARTAAEQGPPSYSSSPPGRSPGPPVRSARRYHRSPAQSPASSRTHKSPPRSPSSPVRSRSATRMLRTAGVHIIREQEEANSVEIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H5T3</accession>
    <name>A0A452H5T3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome non-ATPase regulatory subunit 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">26S Proteasome regulatory subunit RPN11</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD4, a base containing 6 ATPases and few additional components. Within the complex, PSMD4 interacts with subunit PSMD7 through their respective MPN domain. Interacts with TXNL1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M67A family. PSMD14 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H5T3"/>
    <dbReference type="SMR" id="A0A452H5T3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010008"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011490.1">
        <property type="protein sequence ID" value="ENSGAGP00000010008.1"/>
        <property type="gene ID" value="ENSGAGG00000007861.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022624">
        <property type="term" value="C:proteasome accessory complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08069">
        <property type="entry name" value="MPN_RPN11_CSN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000001">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056263">
        <property type="entry name" value="RPN11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23594">
        <property type="entry name" value="RPN11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="13">
        <location>
            <begin position="31"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="310" mass="34567" checksum="B7A9F966D37822EB" modified="2019-05-08" version="1">MDRLLRLGGGMPGLGQGPPTDAPAVDTAEQVYISSLALLKMLKHGRAGVPMEVMGLMLGEFVDDYTVRVIDVFAMPQSGTGVSVEAVDPVFQAKMLDMLKQTGRPEMVVGWYHSHPGFGCWLSGVDINTQQSFEALSERAVAVVVDPIQSVKGKVVIDAFRLINANMMVLGHEPRQTTSNLGHLNKPSIQALIHGLNRHYYSITINYRKNELEQKMLLNLHKKSWMEGLTLQDYSEHCKLNETVVKEMLELAKNYNKAVEEEDKMTPEQLAIKNVGKQDPKRHLEEHVDVLMTSNIVQCLAAMLDTVVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7P4</accession>
    <name>A0A452H7P4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H7P4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010572"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012134.1">
        <property type="protein sequence ID" value="ENSGAGP00000010572.1"/>
        <property type="gene ID" value="ENSGAGG00000008263.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009838">
        <property type="term" value="P:abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044878">
        <property type="term" value="P:mitotic cytokinesis checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19817">
        <property type="entry name" value="Bbox1_ANCHR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15749">
        <property type="entry name" value="FYVE_ZFY19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044553">
        <property type="entry name" value="Bbox1_ANCHR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46603">
        <property type="entry name" value="ABSCISSION/NOCUT CHECKPOINT REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46603:SF1">
        <property type="entry name" value="ABSCISSION_NOCUT CHECKPOINT REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22586">
        <property type="entry name" value="ANCHR-like_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="231"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="417" mass="46242" checksum="3995F8530C3F5491" modified="2019-05-08" version="1">MRGSQQCGCKNCGRAFCSGCLGFSALVPRYGNTQQKVCKQCHGELSRGVSQTSTARWSPPENYKKRVAVLEAKQNQPMGLQKGPVKPQGGAGSRYQGLSKEDRAIAERLERLKEETKPKSIPSQAEIESRLAALKDDPSKPIPSTREMEDRLAALQGRALPSKTPRPVHQPPDTRTQVQQSDDLLIQLAEEVAIDESYSPGAQPQAVNTQTLNYLNRRSEGGSSTADLDPKQLEEEKNKLLAEAAAELREENTREEKILEVAKKLAALRGQDPEKVTLEDYKLPDSDEELTEEEAIQRVLKQLTEEAALDEASGFNIAADQATHPATSQQNTAKKAKLKIQAPTATASAKKPIPIAKAEDSDEEELPWCCICNENATLRCHDCDDDLYCQRCFREGHDEFDRKEHCTSSYRPPCKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9D6</accession>
    <name>A0A452H9D6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Synthesis and degradation of fructose 2,6-bisphosphate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H9D6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011187"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012810.1">
        <property type="protein sequence ID" value="ENSGAGP00000011187.1"/>
        <property type="gene ID" value="ENSGAGG00000008654.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043540">
        <property type="term" value="C:6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000001">
        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF15">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE_FRUCTOSE-2,6-BISPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="8">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="8">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="227" mass="26224" checksum="3019F7DAA69DA32A" modified="2019-05-08" version="1">MNIHVTPRAIYLTRHGESQHNLQGRIGGDSGLSPRGKQFALALASFIESQNIRELKVWTSHMKRTIQTAEALHVPYEQWKALNEIDAGVCEEMSYEEIQAHHPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSAEELPYLKCPLHTVLKLTPVAYGCQVESIFLNVEAVNTHRERPQNVDVTREPEEALGTVPPHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAI6</accession>
    <name>A0A452HAI6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Arylsulfatase K</fullName>
            <ecNumber evidence="12">3.1.6.1</ecNumber>
            <ecNumber evidence="14">3.1.6.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Glucuronate-2-sulfatase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the hydrolysis of pseudosubstrates such as p-nitrocatechol sulfate and p-nitrophenyl sulfate. Catalyzes the hydrolysis of the 2-sulfate groups of the 2-O-sulfo-D-glucuronate residues of chondroitin sulfate, heparin and heparitin sulfate. Acts selectively on 2-sulfoglucuronate and lacks activity against 2-sulfoiduronate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Hydrolysis of the 2-sulfate groups of the 2-O-sulfo-D-glucuronate residues of chondroitin sulfate, heparin and heparitin sulfate.</text>
            <dbReference type="EC" id="3.1.6.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>an aryl sulfate + H2O = a phenol + sulfate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17261"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:33853"/>
            <dbReference type="ChEBI" id="CHEBI:140317"/>
            <dbReference type="EC" id="3.1.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.6.1" evidence="12"/>
    <dbReference type="EC" id="3.1.6.18" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A452HAI6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011740"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013447.1">
        <property type="protein sequence ID" value="ENSGAGP00000011740.1"/>
        <property type="gene ID" value="ENSGAGG00000009059.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015024">
        <property type="term" value="F:glucuronate-2-sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16171">
        <property type="entry name" value="ARSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000039">
        <property type="entry name" value="arylsulfatase K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047892">
        <property type="entry name" value="ARSK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051849">
        <property type="entry name" value="GAG-degrading_sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032506">
        <property type="entry name" value="SGSH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46615">
        <property type="entry name" value="ARYLSULFATASE K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46615:SF1">
        <property type="entry name" value="ARYLSULFATASE K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16347">
        <property type="entry name" value="SGSH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019016867" description="Arylsulfatase K" evidence="19">
        <location>
            <begin position="32"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="20">
        <location>
            <begin position="48"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="N-sulphoglucosamine sulphohydrolase C-terminal" evidence="21">
        <location>
            <begin position="411"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF16347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="578" mass="66542" checksum="68D66854D4A547DF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HB79</accession>
    <name>A0A452HB79_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HB79"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012010"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013754.1">
        <property type="protein sequence ID" value="ENSGAGP00000012010.1"/>
        <property type="gene ID" value="ENSGAGG00000009226.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030172">
        <property type="term" value="F:troponin C binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031013">
        <property type="term" value="F:troponin I binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000001">
        <property type="entry name" value="Troponin T, fast skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027707">
        <property type="entry name" value="TNNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521">
        <property type="entry name" value="TROPONIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521:SF5">
        <property type="entry name" value="TROPONIN T, CARDIAC MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="107"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="107"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="165"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="267" mass="31384" checksum="4559F5603ED092C8" modified="2019-05-08" version="1">MEQRGSGGARPITDIYFEVESAINSVFLFGSQGSAGESKPKPKLFMPNLVPPKIPGGEKVDFDDIHRKRMEKDLNELQGLIDAHFENRKKEEEELISLKDRIEHRRAERAEQQRIRSEREKERQARMAEERARREEEEARKKAEEEARKKKAFSNMMHFGGYLQKTEKKGGKKQTEREKKKKILSERRKPLNIDHLNEDKLRDKAKELWQSIHDLEAEKFELQEKFKRQKYEVRVVLTSASSCGPISAVGRRLRLLYPACHLHHRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBN7</accession>
    <name>A0A452HBN7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxiredoxin-6</fullName>
            <ecNumber evidence="12">1.11.1.27</ecNumber>
            <ecNumber evidence="13">2.3.1.23</ecNumber>
            <ecNumber evidence="2">3.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">1-Cys peroxiredoxin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Acidic calcium-independent phospholipase A2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Glutathione-dependent peroxiredoxin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Lysophosphatidylcholine acyltransferase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Non-selenium glutathione peroxidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41223"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:41224"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + CoA</text>
            <dbReference type="Rhea" id="RHEA:35983"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:35984"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphocholine + CoA</text>
            <dbReference type="Rhea" id="RHEA:12937"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="2.3.1.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a hydroperoxide + 2 glutathione = an alcohol + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62632"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.27" evidence="12"/>
    <dbReference type="EC" id="2.3.1.23" evidence="13"/>
    <dbReference type="EC" id="3.1.1.4" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452HBN7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012175"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013944.1">
        <property type="protein sequence ID" value="ENSGAGP00000012175.1"/>
        <property type="gene ID" value="ENSGAGG00000009370.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1020.10:FF:000001">
        <property type="entry name" value="1-Cys peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000011">
        <property type="entry name" value="Peroxiredoxin PRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503">
        <property type="entry name" value="MCG48959-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503:SF4">
        <property type="entry name" value="PEROXIREDOXIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 21" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 21" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7 21" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="23">
        <location>
            <begin position="4"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="22">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="223" mass="25057" checksum="D62AA8570A14E7C2" modified="2019-05-08" version="1">MPGLLLGDEAPNFEADTTQGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFRKRNVKMIALSIDKVSDHLAWSKDINAYNGDEPTEELPFPIIADAKRELSIQLGMLDPDERDKEGLPLTARVVFIFGPDKKLKLSILYPATTGRNFDEILRVVDSLQLTAKSKVATPVDWKRGDSVMVIPSMPDDEARKLFPEGVFTKELPSGKKYLRYTPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HC26</accession>
    <name>A0A452HC26_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">VWFD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000012324"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014113.1">
        <property type="protein sequence ID" value="ENSGAGP00000012324.1"/>
        <property type="gene ID" value="ENSGAGG00000009479.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000055">
        <property type="entry name" value="alpha-tectorin isoform X1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000153">
        <property type="entry name" value="MUC5B isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050780">
        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339">
        <property type="entry name" value="EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339:SF244">
        <property type="entry name" value="IGGFC-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="VWFD" evidence="6">
        <location>
            <begin position="19"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="6">
        <location>
            <begin position="422"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="6">
        <location>
            <begin position="815"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="6">
        <location>
            <begin position="1198"/>
            <end position="1365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1545" mass="167338" checksum="8F8FCD2D93E73C06" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCM2</accession>
    <name>A0A452HCM2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Importin-11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Ran-binding protein 11</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with UBE2E3 and RPL12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HCM2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012536"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014364.1">
        <property type="protein sequence ID" value="ENSGAGP00000012536.1"/>
        <property type="gene ID" value="ENSGAGG00000008880.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000116">
        <property type="entry name" value="importin-11 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF7">
        <property type="entry name" value="IMPORTIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="14">
        <location>
            <begin position="28"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="887" mass="102299" checksum="407008EB12BAF8B6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HD48</accession>
    <name>A0A452HD48_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HD48"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012745"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014609.1">
        <property type="protein sequence ID" value="ENSGAGP00000012745.1"/>
        <property type="gene ID" value="ENSGAGG00000009778.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036038">
        <property type="term" value="C:MKS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019170">
        <property type="entry name" value="Meckelin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21274">
        <property type="entry name" value="MECKELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21274:SF2">
        <property type="entry name" value="MECKELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09773">
        <property type="entry name" value="Meckelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019566622" evidence="2">
        <location>
            <begin position="24"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="552"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="677"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="718"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="927"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="983" mass="110054" checksum="57E5B1CCB117C450" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HD75</accession>
    <name>A0A452HD75_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HD75"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012727"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014589.1">
        <property type="protein sequence ID" value="ENSGAGP00000012727.1"/>
        <property type="gene ID" value="ENSGAGG00000009766.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47303">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47303:SF1">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="138"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="252"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="286"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="375" mass="39637" checksum="10C73FD8FAA08FAB" modified="2019-05-08" version="1">MAELGEAKRPEAGEDWCDSGLGSLSEGQLSQIQDGLGAAPHPPAAAVTSDPGPGGRPLTSDPGGPRTPDSCGSGCERLDSALGEEEAGWGAAVGRAVGAITQGVEAVRLGEPAARAGETPAVAPQAWLHHVLGFVTEDGDTALHLAVIHEHEAFLDSILQYTAGTEYLDLQNDLGQTALHIAVILGASNFVRKLMAAGAGLCMQEKAGHTALHLACREGWRDCAQWLLASLSGHRAGEGNDACAQLDCTNYDGYTPLHVAVLRKDLEVIKLLVGAGADLNKAELSCGRSPLHLAVESQSPEVVECLLRAGADPRARMYVGYTPIYSAVHRPNQKILQLLREFGSEEPDWDLEESLADSSDEEYDDIVINSGHYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHI1</accession>
    <name>A0A452HHI1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">PR domain-containing protein 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">May be involved in transcription regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HHI1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014336"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016402.1">
        <property type="protein sequence ID" value="ENSGAGP00000014335.1"/>
        <property type="gene ID" value="ENSGAGG00000010898.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000016403.1">
        <property type="protein sequence ID" value="ENSGAGP00000014336.1"/>
        <property type="gene ID" value="ENSGAGG00000010898.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19195">
        <property type="entry name" value="PR-SET_PRDM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000014">
        <property type="entry name" value="PR domain-containing protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044405">
        <property type="entry name" value="PRDM11_PR/SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057456">
        <property type="entry name" value="Znf_C17orf113"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880:SF11">
        <property type="entry name" value="PR_SET DOMAIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25431">
        <property type="entry name" value="zf-C17orf113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="SET" evidence="16">
        <location>
            <begin position="114"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="251"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="251"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00055438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1184" mass="135575" checksum="4CA146707EE8651E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJD1</accession>
    <name>A0A452HJD1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cx9C motif-containing protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mature T-cell proliferation 1 neighbor protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CMC4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJD1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015020"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017156.1">
        <property type="protein sequence ID" value="ENSGAGP00000015020.1"/>
        <property type="gene ID" value="ENSGAGG00000011351.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1130:FF:000002">
        <property type="entry name" value="cx9C motif-containing protein 4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027179">
        <property type="entry name" value="CMC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15590">
        <property type="entry name" value="CX9C MOTIF-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15590:SF0">
        <property type="entry name" value="CX9C MOTIF-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08991">
        <property type="entry name" value="CMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00080130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR627179-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="75" mass="8475" checksum="6728141C33A38EEF" modified="2019-05-08" version="1">MPQKDPCQKQACEIQKCLQANNYLEARCEAVLQEMRRCCAQYPKGRSICCSGFEKEGREREKLKAASNGIHAAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJP8</accession>
    <name>A0A452HJP8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase I</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HJP8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015141"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017298.1">
        <property type="protein sequence ID" value="ENSGAGP00000015141.1"/>
        <property type="gene ID" value="ENSGAGG00000011433.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="8008" checksum="6DDE311561555C88" modified="2019-05-08" version="1">MDHSNREKDERQRPAKGIPGRSGHGSRPSSATSSGVLMVGPNFRVGKKIGCGNFGELRLGKNIAVLVACIIGAYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJR2</accession>
    <name>A0A452HJR2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anamorsin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HJR2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015133"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017287.1">
        <property type="protein sequence ID" value="ENSGAGP00000015133.1"/>
        <property type="gene ID" value="ENSGAGG00000011423.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000085">
        <property type="entry name" value="Anamorsin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007785">
        <property type="entry name" value="Anamorsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049011">
        <property type="entry name" value="Anamorsin_N_metazoan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273:SF14">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20922">
        <property type="entry name" value="Anamorsin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anamorsin N-terminal" evidence="8">
        <location>
            <begin position="9"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="190" mass="20332" checksum="AC24B01209493535" modified="2019-05-08" version="1">MAEYGISAGQHVAVIWDSSSPVEGLKNMVAKIQALVGPESRVSVENIDQLSHSAHAKSSFDVVLSGMVPGSMTLHSAEVLAEIARILKPGGCVLLKEPVAAQIGDNSRIKTAARLLTALMLSGLVEVKEVCVYALTTSRELPFQDCMVFLHAMLTCGYGNSVSSPQRMILVLKRGQPCGLSENPLPSWWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNK0</accession>
    <name>A0A452HNK0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Uridine-cytidine kinase</fullName>
            <ecNumber evidence="10">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452HNK0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016557"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018895.1">
        <property type="protein sequence ID" value="ENSGAGP00000016557.1"/>
        <property type="gene ID" value="ENSGAGG00000012381.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043771">
        <property type="term" value="F:cytidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000297">
        <property type="entry name" value="Uridine-cytidine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="276" mass="31078" checksum="CECE00D84FF6D6CF" modified="2019-05-08" version="1">MASAGSSLGGGEAERPHPRPFLIGVSGGTASGKSTVCEKIMELLGQNEVDHHQRKLIILSQDRFYKVLTADQKAKALKGQYNFDHPGAFDNDLMHTTLKNIVEGKTVEVPTYDFVTHSRLAETTVVYPADVVLFEGILVFYNQDIRDMFHLRLFVDTDSDVRLSRRVLRDMKRGRDLEQILTQYTTFVKPAFEEFCLPTKKYADVIIPRGVDNMVAINLIVQHIQDILNGDICKWQRGAMNGHGRTYKRSFPEQAEGSTMLAAGKRSHLESSSRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HP63</accession>
    <name>A0A452HP63_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Netrin-G2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Laminet-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with LRRC4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
            <orientation evidence="1">Extracellular side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HP63"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016764"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019137.1">
        <property type="protein sequence ID" value="ENSGAGP00000016764.1"/>
        <property type="gene ID" value="ENSGAGG00000012528.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000112">
        <property type="entry name" value="Netrin G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000005">
        <property type="entry name" value="Netrin G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000180">
        <property type="entry name" value="Netrin G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000439">
        <property type="entry name" value="Netrin G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:001530">
        <property type="entry name" value="Netrin-G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF27">
        <property type="entry name" value="NETRIN-G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="13 19" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 20" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019143819" description="Netrin-G2" evidence="20">
        <location>
            <begin position="18"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="23">
        <location>
            <begin position="35"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="22">
        <location>
            <begin position="410"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="453"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="410"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="430"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="478"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="521" mass="59154" checksum="11E8614F4615CE4A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQA7</accession>
    <name>A0A452HQA7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Endoplasmic reticulum aminopeptidase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">ARTS-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Adipocyte-derived leucine aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Aminopeptidase PILS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Puromycin-insensitive leucyl-specific aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="26">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="26">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Monomer. May also exist as a heterodimer; with ERAP2. Interacts with RBMX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQA7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017215"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019642.1">
        <property type="protein sequence ID" value="ENSGAGP00000017215.1"/>
        <property type="gene ID" value="ENSGAGG00000012832.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000007">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000003">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF156">
        <property type="entry name" value="ENDOPLASMIC RETICULUM AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0391">Immunity</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 26" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9 26" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="30">
        <location>
            <begin position="189"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="28">
        <location>
            <begin position="410"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="29">
        <location>
            <begin position="731"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="25">
        <location>
            <position position="483"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="486"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="505"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="27">
        <location>
            <position position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00063353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00069447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00080909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00081523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00081894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00083408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1071" mass="121596" checksum="066243C2933A2136" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQB6</accession>
    <name>A0A452HQB6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQB6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017225"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019652.1">
        <property type="protein sequence ID" value="ENSGAGP00000017225.1"/>
        <property type="gene ID" value="ENSGAGG00000012835.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001997">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000056">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000566">
        <property type="entry name" value="zinc finger protein 133 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000759">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000987">
        <property type="entry name" value="Zinc finger protein 275"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000352">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001035">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408:SF34">
        <property type="entry name" value="ZINC FINGER PROTEIN 672-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="213"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="241"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="381"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="409"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="437"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="465"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="493"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="521"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="549"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="577"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="605"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="55"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="637" mass="72118" checksum="DB063ECB35BCAE49" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQH1</accession>
    <name>A0A452HQH1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HQH1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017276"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019706.1">
        <property type="protein sequence ID" value="ENSGAGP00000017276.1"/>
        <property type="gene ID" value="ENSGAGG00000012861.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000019713.1">
        <property type="protein sequence ID" value="ENSGAGP00000017278.1"/>
        <property type="gene ID" value="ENSGAGG00000012861.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045111">
        <property type="term" value="C:intermediate filament cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026081">
        <property type="entry name" value="DISC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14332">
        <property type="entry name" value="DISRUPTED IN SCHIZOPHRENIA 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14332:SF3">
        <property type="entry name" value="DISRUPTED IN SCHIZOPHRENIA 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="233"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="744"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="475"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="624"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="744"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="853" mass="96270" checksum="721FD589197947F5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQR3</accession>
    <name>A0A452HQR3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HQR3"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019833.1">
        <property type="protein sequence ID" value="ENSGAGP00000017385.1"/>
        <property type="gene ID" value="ENSGAGG00000012937.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019553658" evidence="1">
        <location>
            <begin position="28"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="222" mass="24962" checksum="C7B222BA2CC570C2" modified="2019-05-08" version="1">MKYNSHLLWRMLFFISTVWLITDFIEAGTADCKNADLSDLKHHVKKFSQCFLEMLPDDKRTEINSLAWSLQQVLDRLREVQGKACNAFMTKNCTVPEVPKNGGLVCVNIDSVHYCKPMCNQGYDFGFLRRSRVYEKCGASTGYSWTTQLVGENRLADCISSPIAVSGVKSAYFPAHMCCQQTIANATAESEQIEIFLGELAEAEEDVSQKREMAIDCILCGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HR23</accession>
    <name>A0A452HR23_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translocon-associated protein subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Signal sequence receptor subunit gamma</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterotetramer of TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAP-gamma family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HR23"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017449"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019900.1">
        <property type="protein sequence ID" value="ENSGAGP00000017449.1"/>
        <property type="gene ID" value="ENSGAGG00000012966.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009779">
        <property type="entry name" value="SSR3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13399:SF3">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13399">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN TRAP , GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07074">
        <property type="entry name" value="TRAP-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="187" mass="21221" checksum="737D89358A40E0E8" modified="2019-05-08" version="1">MAPKGGSGARQQSEEDLLLQDFSRNLSAKSSALFFGNAFIVSAIPIWLYWRIWHMDLVQSAVLYSVMTLVSTYLVAFAYKNVKFVLKHKVAQKREDAVSKEVTRKLSEADNRKMSRKEKDERILWKKNEVADYEATTFSIFYNNTLFLVLVIIASFFLLKNFNPTVNYILSISASSGLIALLSTGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTF0</accession>
    <name>A0A452HTF0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Microtubule-associated protein 1B/S N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HTF0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018392"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020960.1">
        <property type="protein sequence ID" value="ENSGAGP00000018392.1"/>
        <property type="gene ID" value="ENSGAGG00000013616.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031114">
        <property type="term" value="P:regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026074">
        <property type="entry name" value="MAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056617">
        <property type="entry name" value="MAP1B/S_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13843">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13843:SF11">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN 1S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23415">
        <property type="entry name" value="MAPB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019399114" description="Microtubule-associated protein 1B/S N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Microtubule-associated protein 1B/S N-terminal" evidence="2">
        <location>
            <begin position="43"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="103" mass="10831" checksum="1FBBC56E21C3245B" modified="2019-05-08" version="1">NAKISPWESLRWLVSVSLVPLLAVSGWDGLCTPLRAVGADCSLSPGIRCWDIDLASCNLDEQLKLFVSRHSATFSSIVKASLSSDMGRAEAPVGLGSNAIGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUP5</accession>
    <name>A0A452HUP5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HUP5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018797"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021428.1">
        <property type="protein sequence ID" value="ENSGAGP00000018797.1"/>
        <property type="gene ID" value="ENSGAGG00000013909.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17211">
        <property type="entry name" value="RA_RGL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF367">
        <property type="entry name" value="RAL GUANINE NUCLEOTIDE DISSOCIATION STIMULATOR-LIKE 2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="22"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="5">
        <location>
            <begin position="405"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="354"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="392"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="526" mass="57427" checksum="842E672CCF280FC2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HW83</accession>
    <name>A0A452HW83_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HW83"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019419"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022117.1">
        <property type="protein sequence ID" value="ENSGAGP00000019419.1"/>
        <property type="gene ID" value="ENSGAGG00000014331.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040091">
        <property type="entry name" value="LRRC56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22708">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22708:SF0">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="417" mass="46065" checksum="E381A6E50F7297B1" modified="2019-05-08" version="1">MNKDWLIVKESIKEGSLAKDISQFDAHPEATRRRPGSGLRPTTAHFMSASRPWTAQRPASAGRSSNVHLLSCGSTLPDSTVSDEIFLEDDSSDLTHGISRVICGNPIKALRARRQKLGSVAMNPFQMPSPKMEHTYDSEEAEDLSREDVFTELRAWREQHNQCLQIIQMEKVPQVLKIHSDEEEDEECSMSDSCEDELRETYDEDLIERISPDSSCQSRLSQSSSDSSHVQESVLPASLNRSLVPSPPKCPSPASIQGVAARPSKVRDIRVRRLKVPNQKEAAQLKEYPQSSVSTEIAAQFVGEEIAILSLHSTLAACEASPLRAEGRYWTSESVGRQTRRPISGPAAVGSTSVRATVDRSPPKVINHHQPVVRSSTQPPERFALRNVVHPLTAKAALQRLPSRPSVSTATQSKMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWU8</accession>
    <name>A0A452HWU8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dehydrogenase/reductase SDR family member 7C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Short-chain dehydrogenase/reductase family 32C member 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HWU8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019677"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022417.1">
        <property type="protein sequence ID" value="ENSGAGP00000019677.1"/>
        <property type="gene ID" value="ENSGAGG00000014519.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05332">
        <property type="entry name" value="11beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000122">
        <property type="entry name" value="Dehydrogenase/reductase SDR family member 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052148">
        <property type="entry name" value="SDR_family_member_7C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44668">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44668:SF2">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="310" mass="34374" checksum="E18943FAA5912CC0" modified="2019-05-08" version="1">MGVLAVLIFPLLLFGISGIIYIYRAVGRLMSKSAVQNKVVVITDAISGLGKECSRVFHSEGARLVLCGKTWEKLEALYNALISVADPSVTFTPKLVLLDLSDINCIQDVAKEILDCYGCVDILINNASLKVKGPVQSISLELDKKIMDANYFGPITLTKALLPSMISRRTGQVVLVNNIQGKLGVPFRAAYAASKHAAQGFFDCLRAEVEEFDVCISTVSPTFIRSYHVQPEPGNWEASIWKFFFRKLAYGVHPMEVAEEVLRTVSRKKQEVFMANAIPKAALYIRTFFPELFFAIIASGVKEKQKVEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXF6</accession>
    <name>A0A452HXF6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HXF6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019896"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022674.1">
        <property type="protein sequence ID" value="ENSGAGP00000019896.1"/>
        <property type="gene ID" value="ENSGAGG00000014658.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034113">
        <property type="term" value="P:heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150079">
        <property type="term" value="P:negative regulation of neuroinflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047164">
        <property type="entry name" value="OX2G-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46841:SF7">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46841">
        <property type="entry name" value="OX-2 MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019317894" description="Ig-like domain-containing protein" evidence="9">
        <location>
            <begin position="24"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="27"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="139" mass="15721" checksum="8244A4615F636FB8" modified="2019-05-08" version="1">MMFTCIFFSWIWAMAAVHRKVQSSKTGEKVTFHCQLVMDHEVLQVTWQKENGEGKGNIATYSRINGHRILGNYSSRVNFTQSELTVSAITVHAVTLQDEGCYRCIFNTFPMGSITGRTCLKVYGTPNSCLQLLHTIVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXU9</accession>
    <name>A0A452HXU9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein transport protein Sec61 subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC61-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HXU9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019948"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022728.1">
        <property type="protein sequence ID" value="ENSGAGP00000019948.1"/>
        <property type="gene ID" value="ENSGAGG00000014692.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030671">
        <property type="entry name" value="Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13509">
        <property type="entry name" value="SEC61 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="9470" checksum="F04133518CAFAD02" modified="2019-05-08" version="1">MPVLPSGAIVGASSGKAVWPKGAGWSVKQRKNNCSASLSTSSSSTKGVWSFYTDASHGIKVGPFTVLIISILFIAFVIILHIWAKYTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXX9</accession>
    <name>A0A452HXX9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribonuclease H2 subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ribonuclease HI subunit B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase H2 subunit B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HXX9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019983"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022767.1">
        <property type="protein sequence ID" value="ENSGAGP00000019983.1"/>
        <property type="gene ID" value="ENSGAGG00000014722.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09270">
        <property type="entry name" value="RNase_H2-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.120:FF:000001">
        <property type="entry name" value="Ribonuclease H2 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.530:FF:000001">
        <property type="entry name" value="Ribonuclease H2 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040456">
        <property type="entry name" value="RNase_H2_suB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019024">
        <property type="entry name" value="RNase_H2_suB_wHTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041195">
        <property type="entry name" value="Rnh202_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383:SF11">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09468">
        <property type="entry name" value="RNase_H2-Ydr279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17745">
        <property type="entry name" value="Ydr279_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rnh202 triple barrel" evidence="9">
        <location>
            <begin position="9"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonuclease H2 subunit B wHTH" evidence="8">
        <location>
            <begin position="67"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="279" mass="31792" checksum="DB78850B01F1800F" modified="2019-05-08" version="1">MKPDRGPLFTRLRNPSTGEAALYLFNSSAQQLFEVKAFHEEYRSWFIGQTVQHDGRLLFATPMDPLFLVLFYLIKAEKEKGKFQPLDQVVVDSEFSSCTMLLQCARVMDSLHHVIEEKEISNKKFYKYSQEKTLKWLKKKVNQTVKVLKSSNVCVGGRVQSATFISGKQITDVKEDDYVRYAHGLISEYIPEDLSVELSKYLQLPELTSPEADPPLKKRKFSDAPLEAEEDYTKFNSSDLKNKKTTSKMSAAQKALAKVDKTGMKTMSAFFSAKPKATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYK4</accession>
    <name>A0A452HYK4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dynein heavy chain, cytosolic</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000020251"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023074.1">
        <property type="protein sequence ID" value="ENSGAGP00000020251.1"/>
        <property type="gene ID" value="ENSGAGG00000014928.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000122">
        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000002">
        <property type="entry name" value="cytoplasmic dynein 1 heavy chain 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000001">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000005">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000003">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000001">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000071">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000517">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000003">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000004">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000004">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000373">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001956">
        <property type="entry name" value="Dynein cytoplasmic 1 heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002655">
        <property type="entry name" value="Dynein cytoplasmic 1 heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532:SF13">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 HEAVY CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532">
        <property type="entry name" value="MALE FERTILITY FACTOR KL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="1823"/>
            <end position="1967"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="2138"/>
            <end position="2289"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="2490"/>
            <end position="2640"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="2832"/>
            <end position="2998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2312"/>
            <end position="2332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1095"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="3108"/>
            <end position="3156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="3305"/>
            <end position="3402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="4549" mass="522527" checksum="C5AA9941C6BD5D9C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZV7</accession>
    <name>A0A452HZV7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="24">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(out) + 2 chloride(out) + Na(+)(out) = K(+)(in) + 2 chloride(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72395"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:72396"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC12A transporter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZV7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020730"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023610.1">
        <property type="protein sequence ID" value="ENSGAGP00000020730.1"/>
        <property type="gene ID" value="ENSGAGG00000015263.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008511">
        <property type="term" value="F:sodium:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055078">
        <property type="term" value="P:sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000005">
        <property type="entry name" value="Solute carrier family 12 member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013612">
        <property type="entry name" value="AA_permease_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002445">
        <property type="entry name" value="Slc12a1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002443">
        <property type="entry name" value="SLC12A1/SLC12A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00930">
        <property type="entry name" value="2a30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF93">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08403">
        <property type="entry name" value="AA_permease_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01207">
        <property type="entry name" value="NAKCLTRNSPRT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01209">
        <property type="entry name" value="NAKCLTRSPRT2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0868">Chloride</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0915">Sodium</keyword>
    <keyword evidence="15" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="579"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="612"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease N-terminal" evidence="23">
        <location>
            <begin position="87"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="21">
        <location>
            <begin position="192"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="22">
        <location>
            <begin position="703"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="833"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF08403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1108" mass="122694" checksum="1276850B32984A2B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I313</accession>
    <name>A0A452I313_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I313"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021902"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024949.1">
        <property type="protein sequence ID" value="ENSGAGP00000021902.1"/>
        <property type="gene ID" value="ENSGAGG00000016073.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003495">
        <property type="entry name" value="Ras homolog family member Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="168" mass="19085" checksum="972F543463A136A7" modified="2019-05-08" version="1">GIRTCLLISPQHARVKFQFRIADNNIFLPQEDYDRLRPLSYPMTDVFLICFSVVNPASFQNVKEEWVPELKEYAPNVPFLLVGTQIDLRDDPKTLARLNDLKEKPVSMEQGQKLAKEIGACCYVECSALTQKGLKTVFDEAIIAILTPKKHTVKKRIGSRCINCCLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3R0</accession>
    <name>A0A452I3R0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 2 subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 2 subunit beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3R0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022152"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025250.1">
        <property type="protein sequence ID" value="ENSGAGP00000022152.1"/>
        <property type="gene ID" value="ENSGAGG00000016270.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="7">
        <location>
            <begin position="200"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="334" mass="38496" checksum="CB2A8010C31E3E31" modified="2019-05-08" version="1">MSGDEMIFDPTMSKKKKKKKKPFVLDEEGGETQAEETQLSETKEVEPEPTEDKNIEADEEDSRKKDASDDLDDLNFFNQKKKKKKTKKMFDIDEAEEGIKDLKIEGDFQELVEPEDDLDIMLGNKKKKKKNMKFPDEDEILEKDEALEDEDSKKDDGISFSFHTGPAWAGSERDYTYDELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4K0</accession>
    <name>A0A452I4K0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I4K0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022491"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025621.1">
        <property type="protein sequence ID" value="ENSGAGP00000022491.1"/>
        <property type="gene ID" value="ENSGAGG00000016495.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050769">
        <property type="entry name" value="NAT_camello-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13947:SF58">
        <property type="entry name" value="8B (PUTATIVE,_PSEUDO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13947">
        <property type="entry name" value="GNAT FAMILY N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="31"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="177" mass="19807" checksum="A13CFD8A76754E57" modified="2019-05-08" version="1">MLRCLQTQLLFLGLFLAVLAASESLLVSFLALLLTLIGGWFCIRSLWTDYVHQSLRSDLLDIRRTYLEAADSCLWVAEVEGAVVGAHVSVRREHRGRGIGRALCRTVVRFTQECGYSAVVLSTSMVQYSALQHGLPEGLGEVPIPPRHRATDHYPLSPTIKPAFYPPYNLFIQPIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5H6</accession>
    <name>A0A452I5H6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000022817"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025997.1">
        <property type="protein sequence ID" value="ENSGAGP00000022817.1"/>
        <property type="gene ID" value="ENSGAGG00000016725.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46459:SF1">
        <property type="entry name" value="E1A-BINDING PROTEIN P400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46459">
        <property type="entry name" value="E1A-BINDING PROTEIN P400-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="503"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="666" mass="77358" checksum="6A7CA706568EA724" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5K5</accession>
    <name>A0A452I5K5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I5K5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022823"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026004.1">
        <property type="protein sequence ID" value="ENSGAGP00000022823.1"/>
        <property type="gene ID" value="ENSGAGG00000016723.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126:SF14">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126">
        <property type="entry name" value="ANKYRIN REPEAT, PH AND SEC7 DOMAIN CONTAINING PROTEIN SECG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="24"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="70"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="103"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="136"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="169"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="202"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="235"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="268"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="301"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="335"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="368"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="401"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="434"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="467"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="500"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="579"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="612"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="646"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="682"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="715"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="748"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="785"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="852"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="885"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="955"/>
            <end position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1083" mass="116308" checksum="EAAB7E1B5EB7418F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6P0</accession>
    <name>A0A452I6P0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000023299"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026540.1">
        <property type="protein sequence ID" value="ENSGAGP00000023299.1"/>
        <property type="gene ID" value="ENSGAGG00000017082.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056306">
        <property type="entry name" value="Ig-CFAP74_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056307">
        <property type="entry name" value="Ig-CFAP74_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056310">
        <property type="entry name" value="Ig-CFAP74_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22538">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22538:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24770">
        <property type="entry name" value="Ig-CFAP74_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24778">
        <property type="entry name" value="Ig-CFAP74_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24798">
        <property type="entry name" value="Ig-CFAP74_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24771">
        <property type="entry name" value="Ig_CFAP74_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CFAP74 second Ig-like" evidence="3">
        <location>
            <begin position="655"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP74 third Ig-like" evidence="4">
        <location>
            <begin position="863"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP74 fourth Ig-like" evidence="5">
        <location>
            <begin position="981"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="720"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="106"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="726"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1368" mass="154563" checksum="3BB11301A4F3A9F7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7E1</accession>
    <name>A0A452I7E1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Inward rectifier potassium channel 16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Inward rectifier K(+) channel Kir5.1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Potassium channel, inwardly rectifying subfamily J member 16</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance. In the kidney, together with KCNJ10, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">It forms heteromeric channels with Kir4.1/KCNJ10; this interaction is required for KCNJ16 localization to the basolateral membrane in kidney cells. As a heteromer with KCNJ10, may interact with MAGI1; this interaction may facilitate KCNJ10/KCNJ16 potassium channel expression at the basolateral membrane in kidney cells. May form heteromers with Kir2.1/KCNJ2. Can form heteromeric channels with Kir4.2/KCNJ15.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 22">Membrane</location>
            <topology evidence="1 22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ16 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I7E1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023549"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026836.1">
        <property type="protein sequence ID" value="ENSGAGP00000023549.1"/>
        <property type="gene ID" value="ENSGAGG00000017263.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000063">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000003">
        <property type="entry name" value="Potassium voltage-gated channel subfamily J member 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008061">
        <property type="entry name" value="K_chnl_inward-rec_Kir5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF24">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01678">
        <property type="entry name" value="KIR5CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 22" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 22" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 22" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 22 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 22" id="KW-0813">Transport</keyword>
    <keyword evidence="8 22" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="24">
        <location>
            <begin position="29"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="25">
        <location>
            <begin position="173"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="21">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00055471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00080035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00080884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR005465-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="409" mass="47268" checksum="6601150265AB0105" modified="2019-05-08" version="1">MQENKLCCQNSSQECTIVRAKKMQKRFLHKDGSCNVYFKHIFGEWESYVADIFTTLVDIKWRHMFVIFSLSYILSWLFFGLVFWLIAIQHGDLVHDEEITPCVAKVHSFTGAFLFSLETQTTIGFGFRCVTEECSVAILMVVLQSVLSCIIDTFIIGAALAKMATARKRAQTIRFSYYATVGLRDDKLCLMWRIGDFRPNHMVEGTVRAQLLRYMEDMEGRMTMEYKDLKLLNDQVILVTPVTIVHEIDHDSPLYDLDRKALAKDSFEILVTFVYTGDSTGTSHQSRSSYVPREILWGHWFNNVLQVKKKYYNVDCLQFEETTEVYAPYCSAKQLDRKEHEWNRTKKTLDRGTSTSTLEVRETSFKAVALISSSEDPEDLMKAVNQDSRELPYQKDPLTLNRISAKSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7S9</accession>
    <name>A0A452I7S9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I7S9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023587"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026875.1">
        <property type="protein sequence ID" value="ENSGAGP00000023587.1"/>
        <property type="gene ID" value="ENSGAGG00000017288.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001230">
        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="89"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="212" mass="22511" checksum="46254B94EC69F50A" modified="2019-05-08" version="1">MTQTPETLAVSPGASVTINCKASSSIGNAIAWYQQKSGQAPKLFVYGPSTRPSGIPDRFSGTGNGNTDYSLTIRSVEADDNGDYYYSYGQIVLTQTPESLAVFPGDRVTINCKATSTSVGSSVHWYQQKPGQAPKLLIYGASNLQSGVPARFSGSGSGTDFTFTISSVEAEDAGDYFCQQSSSWPLTVIQTNTKTSLLSAACISVTICCRYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9P0</accession>
    <name>A0A452I9P0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fibroblast growth factor</fullName>
            <shortName evidence="6">FGF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I9P0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024404"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027775.1">
        <property type="protein sequence ID" value="ENSGAGP00000024404.1"/>
        <property type="gene ID" value="ENSGAGG00000017830.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23326">
        <property type="entry name" value="beta-trefoil_FGF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000004">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0339">Growth factor</keyword>
    <keyword evidence="5" id="KW-0497">Mitogen</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="207" mass="23693" checksum="CCF267E38E14B293" modified="2019-05-08" version="1">MAEVGGFLGSLDLDLHGFSSSLGNVALADSPGFLNERLGQIEGRLQRGSPTDFAHLKGILRRRQLYCRTGFHLEIFPNGTVHGTRQDHSRFGILEFISLAVGLVSIRGVDSGLYLGMNERGELYGSKKLTRECVFREQFEENWYNTYASTLYKHSDSERQYYVALNKDGSPREGYRTKRHQKFTHFLPRPVDPAKIPAMFRDLFHYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAK7</accession>
    <name>A0A452IAK7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>3-sulfino-L-alanine(out) + L-aspartate(in) = 3-sulfino-L-alanine(in) + L-aspartate(out)</text>
            <dbReference type="Rhea" id="RHEA:70975"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3-sulfino-L-alanine(out) + L-glutamate(in) + H(+)(in) = 3-sulfino-L-alanine(in) + L-glutamate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70967"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-aspartate(in) + L-glutamate(out) + H(+)(out) = L-aspartate(out) + L-glutamate(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70783"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer (via N-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IAK7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024748"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028160.1">
        <property type="protein sequence ID" value="ENSGAGP00000024748.1"/>
        <property type="gene ID" value="ENSGAGG00000018066.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015183">
        <property type="term" value="F:L-aspartate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043490">
        <property type="term" value="P:malate-aspartate shuttle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000004">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000064">
        <property type="entry name" value="calcium-binding mitochondrial carrier protein Aralar1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051028">
        <property type="entry name" value="Mito_Solute_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678:SF12">
        <property type="entry name" value="ELECTROGENIC ASPARTATE_GLUTAMATE ANTIPORTER SLC25A13, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="17">
        <location>
            <begin position="325"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="17">
        <location>
            <begin position="425"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="17">
        <location>
            <begin position="517"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="675" mass="74002" checksum="3A5230357651FFC9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IEU5</accession>
    <name>A0A452IEU5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IEU5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026388"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030000.1">
        <property type="protein sequence ID" value="ENSGAGP00000026388.1"/>
        <property type="gene ID" value="ENSGAGG00000019237.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF38">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="235" mass="26431" checksum="53131EE479409721" modified="2019-05-08" version="1">MELPNKAAEFPNSAYPPGYAGNQVPYGYPQYPFQILTGFETNNKYEIKNSLGQRVYFAAEENDCCTRNCCGPSRPFTMKIIDNMGQEVIALQRPLRCSACCCPCCLQELEVQAPPGTPVGYIKQNWHPCLPKFTVQNEAKIDVFKIIGPCVVCSCYADINFEVKTMDESSVVGRISKQWSGFVREAFTDADNFGIQFPLDLDVKMKAVMIGACFLIDFMFFEQTGNKQQRAGVWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IF09</accession>
    <name>A0A452IF09_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IF09"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026459"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030074.1">
        <property type="protein sequence ID" value="ENSGAGP00000026459.1"/>
        <property type="gene ID" value="ENSGAGG00000019278.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017156">
        <property type="term" value="P:calcium-ion regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08385">
        <property type="entry name" value="C2A_Synaptotagmin-1-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08403">
        <property type="entry name" value="C2B_Synaptotagmin-3-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000005">
        <property type="entry name" value="Synaptotagmin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000011">
        <property type="entry name" value="Synaptotagmin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF46">
        <property type="entry name" value="SYNAPTOTAGMIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="C2" evidence="10">
        <location>
            <begin position="49"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="10">
        <location>
            <begin position="181"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="341" mass="38827" checksum="CDF4E3DBBCE66519" modified="2019-05-08" version="1">MQVSSVDFNMGTDPILQRGETTTSIGRIKPELYKQKSVDSEDKQEDVKICGKLNFTLRYDYENELLVVTIVKALDLPAKDFTGTSDPYVKIYLLPDRKKKFQTRVHRKTLNPVFDETFQFPVAYDQLSNRKLHFSVYDFDRFSRHDMIGEVILDNFFEVSDLSREATVWKDIHCATTESIDLGEIMFSLCYLPTAGRMTLTVIKCRNLKAMDVTGASDPYVKVSLMCEGRRLKKRKTTTKKNTLNPVYNEAIIFDIPPENVDQVSLSIAVMDYDRVGHNEVIGVCRTGLDAEGLGRDHWNEMLAYPRKPITHWHPLVELPGQATSFDSQGSCSSPKPPPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFT1</accession>
    <name>A0A452IFT1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM MRD1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IFT1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026719"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030366.1">
        <property type="protein sequence ID" value="ENSGAGP00000026719.1"/>
        <property type="gene ID" value="ENSGAGG00000019457.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12567">
        <property type="entry name" value="RRM3_RBM19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12569">
        <property type="entry name" value="RRM4_RBM19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12318">
        <property type="entry name" value="RRM5_RBM19_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12571">
        <property type="entry name" value="RRM6_RBM19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000240">
        <property type="entry name" value="RNA binding motif protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000277">
        <property type="entry name" value="RNA binding motif protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000297">
        <property type="entry name" value="RNA binding motif protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000389">
        <property type="entry name" value="RNA binding motif protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034419">
        <property type="entry name" value="RBM19_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034420">
        <property type="entry name" value="RBM19_RRM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034423">
        <property type="entry name" value="RBM19_RRM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034421">
        <property type="entry name" value="RBM19_RRM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051945">
        <property type="entry name" value="RRM_MRD1_RNA_proc_ribogen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039:SF5">
        <property type="entry name" value="RNA-BINDING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="45"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="230"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="358"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="460"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="310"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="585" mass="65364" checksum="3193C43253454AA3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IG07</accession>
    <name>A0A452IG07_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">IP5PC-F beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IG07"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026890"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030566.1">
        <property type="protein sequence ID" value="ENSGAGP00000026890.1"/>
        <property type="gene ID" value="ENSGAGG00000019580.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1490:FF:000014">
        <property type="entry name" value="AGAP008095-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056454">
        <property type="entry name" value="Beta-prop_IP5PC_F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052993">
        <property type="entry name" value="CFA-57"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23754">
        <property type="entry name" value="Beta-prop_IP5PC_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="IP5PC-F beta-propeller" evidence="3">
        <location>
            <begin position="66"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="103"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="163"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="372"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="580"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="679"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="731" mass="87309" checksum="6A3BF26E425CCC10" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGM9</accession>
    <name>A0A452IGM9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IGM9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027076"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030767.1">
        <property type="protein sequence ID" value="ENSGAGP00000027076.1"/>
        <property type="gene ID" value="ENSGAGG00000019700.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01518">
        <property type="entry name" value="RHOD_YceA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000022">
        <property type="entry name" value="Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022111">
        <property type="entry name" value="Rhodanese_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020936">
        <property type="entry name" value="TrhO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040503">
        <property type="entry name" value="TRHO_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057944">
        <property type="entry name" value="TSTD2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43268">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE/RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43268:SF6">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12368">
        <property type="entry name" value="Rhodanese_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23949">
        <property type="entry name" value="TSTD2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17773">
        <property type="entry name" value="UPF0176_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="302"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00069711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="494" mass="55319" checksum="362BE9961DE7DC6A" modified="2019-05-08" version="1">MISSSVLLDGGDEVECSILRFPDLDLKDPLPLTAKSNLKAESDTSERKKYAFAKKKAFALFVKAKEVPATACCPPAVCRAGLGWKCCRQTFKDLTGIHIHVATQHSSDIQQQTGLILKQLAAEARTSSNPASADETGSLSSPTCNIQELSYRLPDTNHISFDELTSGVGEVLLYYCYHEVKDPLRLCAWQKSLCQHLHLTGKVRIASEGINGTVGGSKVATSLYIQAMLSHPLFKTILCREDFKSSAGGAHCFPDLRVGVFEEIVPLGINPNKVSYKETGIHLSPEEFHKEVEKYLSQAPQAQSDTILLDCRNFYESKIGHFQGCLAPDIRKFSYFPSYVDENLELFKNKRVLMYCTGGIRCERGSAYLRTKAACREVYQLKGGIHKYLEEFPDGFYRGKLFVFDQRYTISSNEDIIAECRYCGTLWDQYRVCSSQHCRQLVLTCPSCHAKGFTACCPICQEKGLKLASNPSGWMVKEECECTDRRQRVPVKHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGN6</accession>
    <name>A0A452IGN6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G protein-regulated inducer of neurite outgrowth C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in neurite outgrowth.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IGN6"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030272.1">
        <property type="protein sequence ID" value="ENSGAGP00000026634.1"/>
        <property type="gene ID" value="ENSGAGG00000019395.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026646">
        <property type="entry name" value="GPRIN2-like/GPRIN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032745">
        <property type="entry name" value="GRIN_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15718:SF5">
        <property type="entry name" value="G PROTEIN-REGULATED INDUCER OF NEURITE OUTGROWTH 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15718">
        <property type="entry name" value="G PROTEIN-REGULATED INDUCER OF NEURITE OUTGROWTH C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15235">
        <property type="entry name" value="GRIN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G protein-regulated inducer of neurite outgrowth C-terminal" evidence="3">
        <location>
            <begin position="504"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="560"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="560"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="601" mass="65758" checksum="82D79637AE45CB71" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IID0</accession>
    <name>A0A452IID0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Calcium and integrin-binding family member 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Cilium</location>
            <location evidence="4">Photoreceptor outer segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Stereocilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Photoreceptor inner segment</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IID0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027586"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031340.1">
        <property type="protein sequence ID" value="ENSGAGP00000027586.1"/>
        <property type="gene ID" value="ENSGAGG00000020045.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032420">
        <property type="term" value="C:stereocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055074">
        <property type="term" value="P:calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000035">
        <property type="entry name" value="Calcium and integrin-binding family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051433">
        <property type="entry name" value="CIBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45791">
        <property type="entry name" value="CALCIUM AND INTEGRIN BINDING FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45791:SF5">
        <property type="entry name" value="CALCIUM AND INTEGRIN-BINDING FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="30"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="67"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="108"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="151" mass="17315" checksum="F1A2EDD9DFF512B0" modified="2019-05-08" version="1">MAPNIVPMDYTKDPDVKLPIQLIINMPELKENPFKERIVEAFSEDGDGNLSFNDFVDMFSVLSEMAPRELKAIYAFKIYDFNTDNFICKSDLEKTLNKLTREELTAEEITLVCEKVIEEADMDGDGKLAFADFENMISKAPDFLSTFHIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IK33</accession>
    <name>A0A452IK33_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000028306"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032162.1">
        <property type="protein sequence ID" value="ENSGAGP00000028306.1"/>
        <property type="gene ID" value="ENSGAGG00000020558.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000032163.1">
        <property type="protein sequence ID" value="ENSGAGP00000028307.1"/>
        <property type="gene ID" value="ENSGAGG00000020558.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019459">
        <property type="entry name" value="GRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000237">
        <property type="entry name" value="GRIP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921">
        <property type="entry name" value="MYOSIN HEAVY CHAIN - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921:SF2">
        <property type="entry name" value="THYROID RECEPTOR-INTERACTING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10375">
        <property type="entry name" value="GRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50913">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRIP" evidence="6">
        <location>
            <begin position="1783"/>
            <end position="1832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1904"/>
            <end position="1935"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="66"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="228"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="387"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1026"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1134"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1214"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1347"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1425"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1534"/>
            <end position="1603"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1647"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1910"/>
            <end position="1926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1991" mass="228842" checksum="2813823269993E52" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IL14</accession>
    <name>A0A452IL14_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IL14"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028705"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032602.1">
        <property type="protein sequence ID" value="ENSGAGP00000028705.1"/>
        <property type="gene ID" value="ENSGAGG00000020796.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="4">
        <location>
            <begin position="397"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="123"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="587"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="183"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="616" mass="67862" checksum="037A04D944B3B76D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IL59</accession>
    <name>A0A452IL59_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">small monomeric GTPase</fullName>
            <ecNumber evidence="5">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Phagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452IL59"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028577"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032456.1">
        <property type="protein sequence ID" value="ENSGAGP00000028577.1"/>
        <property type="gene ID" value="ENSGAGG00000020702.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000085">
        <property type="entry name" value="Putative ras-related protein rab-11a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="216" mass="24233" checksum="D902BC966569B0B7" modified="2019-05-08" version="1">MRGKDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDNKTVKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKYLTYENAERWLKELQDHADANIVIMLVGNKSDLRHLRAVPTDEAKSFAEKNGLSFIETSALDSTNVETAFHSILAEIYRIVSQRQMTGQQEPEFGSSTAIDPIKVLPTHQEGKQAPCCQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILA6</accession>
    <name>A0A452ILA6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein delta homolog 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Monomer. Interacts with SH3RF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILA6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028623"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032510.1">
        <property type="protein sequence ID" value="ENSGAGP00000028623.1"/>
        <property type="gene ID" value="ENSGAGG00000020736.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000018">
        <property type="entry name" value="Delta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000095">
        <property type="entry name" value="Notch, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000321">
        <property type="entry name" value="Protein delta homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000486">
        <property type="entry name" value="Protein delta homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000118">
        <property type="entry name" value="protein delta homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049:SF22">
        <property type="entry name" value="DROSOPHILA CRUMBS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21700">
        <property type="entry name" value="EGF_DL_JAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019026291" description="Protein delta homolog 1" evidence="16">
        <location>
            <begin position="33"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="101"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="140"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="183"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="221"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="248"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="395" mass="42650" checksum="C77962D90A6533CE" modified="2019-05-08" version="1">MQVGSAPLICWVMDFQAVTLICCCCCFPLILPVTPDVVCKPGCHPVNGFCEIPSECRCQPGWQGALCNQCVPFPGCVHGSCAKPWQCVCEEGWVGSLCDIDIHPCSSKPCTNNSTCIETGDGGYICLCAKGFTGKNCHLKKGPCIINGSPCQNGGTCVDDNGFAPHASCLCLPSFAGNFCEIDVDDCEPNPCENGGTCTDIGGGFNCHCPIGYMGKSCSSRVIFCASSPCENGGTCHEHPEGGFECICKPEFVGVTCTYLNRNTSLHGVNTEAKHGQSYNLPPNAHPKSVPHQEYKVLKITMKETIQNTDPLLNKSQVICFIVLGLLTCLVVLGTTAIVFFSKCEMWLANAKYSHLLRKKKNCFLQSNNGENLSVNIIFPEKIKLTNYTKNYTAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMB0</accession>
    <name>A0A452IMB0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Mannose receptor C-type 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000029050"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033001.1">
        <property type="protein sequence ID" value="ENSGAGP00000029050.1"/>
        <property type="gene ID" value="ENSGAGG00000020766.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23407">
        <property type="entry name" value="beta-trefoil_Ricin_MRC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00062">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000032">
        <property type="entry name" value="macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000014">
        <property type="entry name" value="Macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000016">
        <property type="entry name" value="macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000023">
        <property type="entry name" value="Macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000031">
        <property type="entry name" value="macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000025">
        <property type="entry name" value="Mannose receptor C-type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000030">
        <property type="entry name" value="Mannose receptor C-type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000027">
        <property type="entry name" value="Mannose receptor, C type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.10">
        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000562">
        <property type="entry name" value="FN_type2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036943">
        <property type="entry name" value="FN_type2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24562">
        <property type="entry name" value="CysR_MRC2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00040">
        <property type="entry name" value="fn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00013">
        <property type="entry name" value="FNTYPEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00059">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51092">
        <property type="entry name" value="FN2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0430">Lectin</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019057165" description="Mannose receptor C-type 1" evidence="16">
        <location>
            <begin position="19"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1382"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="18">
        <location>
            <begin position="163"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="17">
        <location>
            <begin position="228"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="17">
        <location>
            <begin position="371"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="17">
        <location>
            <begin position="513"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="17">
        <location>
            <begin position="657"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="17">
        <location>
            <begin position="807"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="17">
        <location>
            <begin position="951"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="17">
        <location>
            <begin position="1096"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="17">
        <location>
            <begin position="1234"/>
            <end position="1352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1451" mass="164823" checksum="D8BC0504EA715DBF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMP0</accession>
    <name>A0A452IMP0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">IRS-type PTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IMP0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029147"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033109.1">
        <property type="protein sequence ID" value="ENSGAGP00000029147.1"/>
        <property type="gene ID" value="ENSGAGG00000021091.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005104">
        <property type="term" value="F:fibroblast growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005068">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008543">
        <property type="term" value="P:fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01202">
        <property type="entry name" value="PTB_FRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000169">
        <property type="entry name" value="Fibroblast growth factor receptor substrate 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050996">
        <property type="entry name" value="Docking_Protein_DOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038742">
        <property type="entry name" value="FRS2_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258">
        <property type="entry name" value="DOCKING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258:SF40">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR SUBSTRATE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00310">
        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRS-type PTB" evidence="7">
        <location>
            <begin position="13"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="121"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="270"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="441"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="482"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="276"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="325"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="508" mass="57241" checksum="C065434D90C5F94A" modified="2019-05-08" version="1">MGSCCSCPDKETVPDNHRNKFKVINVDDDGNELGSGVMELTDTELILYTRKRDSVKWHYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCARAEELFNMLQEIMQNNSINVVEEPVVERNHHQTELEVPRTPRTPTTPGFSAQSLPNGYPRYPSFGDASSHPSSRHPSVGSARLPSVGEESTHPLLVAEEQVHTYVNTTGVQEEKKNRSSVHVPLELRLSTVETSKTTEDQNCTDDRDAQVLLEPEGVKFVLGPTPVQRQLMEREQLEQLGRDQVSGSSTNTSEWDTGYDSDERKETHSGNKLVYENLNRLSIPSASGVRRGRLTSTSTSDTQNINNSAQRRTALLNYENLPSLPPVWEARKLSRDEDDNLGPKTPSLNGYHNNLDPMHNYVNTENVTVPASAHKVEFTRRRDCTPTVFNFDIRRPSLEHRQLNYIQVDLEGGSDSDNPQTPKTPTTPLPQTPTRRTELYAVIDIERTAAMSSLQKALPRDDGTSRKTRHNSTDLPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRX3</accession>
    <name>A0A452IRX3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IRX3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030608"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034729.1">
        <property type="protein sequence ID" value="ENSGAGP00000030608.1"/>
        <property type="gene ID" value="ENSGAGG00000021876.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000002">
        <property type="entry name" value="Type I keratin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF369">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="7">
        <location>
            <begin position="121"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="222"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="335"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="517" mass="54796" checksum="5BB119344B1514A8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IS20</accession>
    <name>A0A452IS20_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IS20"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030810"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034939.1">
        <property type="protein sequence ID" value="ENSGAGP00000030810.1"/>
        <property type="gene ID" value="ENSGAGG00000022151.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15911">
        <property type="entry name" value="7tmA_OR11A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF366">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="47"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="316" mass="35693" checksum="19FCC6BA549EDE0E" modified="2019-05-08" version="1">GKPNDHHRGENQMTITEFILLGFGDLHELKIPVFMVFLAIYIVTLTGNILIIVMVSYIHNLHNPMYFFLGNLSVLEVCYTTSVAPKMLKTLLVVQEDISFSSFLLQFYIFGSLVVAECFLLAAMSYDRDLAICKPLHYASTMTFQLCFQLAVGSWVTGFLSLVVTMPMVSRLPFCASKEIDHFFCDLTPIIKLSCGDTYMVRILAFIIASFVSFLSFLLILLSYYKIISTILKIPSISGKQKAFSTCSSHLIVMSVFYGTLMVVYAAPIANQWPNLNKIFSVLYTMVTPLINAIVYSLRNMEVKETLRKLFSKNPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ISW2</accession>
    <name>A0A452ISW2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22 24">E3 ubiquitin-protein ligase CBL</fullName>
            <ecNumber evidence="7 24">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6 24">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="24">The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="7 24"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031217"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035406.1">
        <property type="protein sequence ID" value="ENSGAGP00000031217.1"/>
        <property type="gene ID" value="ENSGAGG00000022423.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042059">
        <property type="term" value="P:negative regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16708">
        <property type="entry name" value="RING-HC_Cbl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09920">
        <property type="entry name" value="SH2_Cbl-b_TKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14393">
        <property type="entry name" value="UBA_c-Cbl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000022">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000030">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.20:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000015">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000154">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024162">
        <property type="entry name" value="Adaptor_Cbl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014741">
        <property type="entry name" value="Adaptor_Cbl_EF_hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003153">
        <property type="entry name" value="Adaptor_Cbl_N_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014742">
        <property type="entry name" value="Adaptor_Cbl_SH2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024159">
        <property type="entry name" value="Cbl_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23007">
        <property type="entry name" value="CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23007:SF5">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02262">
        <property type="entry name" value="Cbl_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02761">
        <property type="entry name" value="Cbl_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02762">
        <property type="entry name" value="Cbl_N3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47668">
        <property type="entry name" value="N-terminal domain of cbl (N-cbl)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51506">
        <property type="entry name" value="CBL_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="17 24" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="21" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="18" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="15 24" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="16 24" id="KW-0862">Zinc</keyword>
    <keyword evidence="14 23 24" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Cbl-PTB" evidence="28">
        <location>
            <begin position="30"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="27">
        <location>
            <begin position="368"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="26">
        <location>
            <begin position="854"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="465"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="669"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="742"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="808"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="492"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="25">
        <location>
            <begin position="520"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="626"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="742"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="25">
        <location>
            <begin position="767"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00067639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU367001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="904" mass="99723" checksum="F010BC0905F68855" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IVD7</accession>
    <name>A0A452IVD7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Splicing factor, arginine/serine-rich 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IVD7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032039"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036324.1">
        <property type="protein sequence ID" value="ENSGAGP00000032039.1"/>
        <property type="gene ID" value="ENSGAGG00000022965.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12597">
        <property type="entry name" value="RRM1_SRSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12767">
        <property type="entry name" value="RRM2_SRSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000053">
        <property type="entry name" value="Serine/arginine-rich splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000170">
        <property type="entry name" value="Serine/arginine-rich splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034520">
        <property type="entry name" value="SRSF1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029538">
        <property type="entry name" value="SRSF1_RRM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF66">
        <property type="entry name" value="SERINE_ARGININE-RICH SPLICING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="16"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="121"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="88"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="193"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="205"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="253" mass="28376" checksum="B4AC05DB82E3F351" modified="2019-05-08" version="1">MSGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGGGGGGAPRGRYGPPSRRSEYRVIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSRSNSRSRSYSPRRSRGSPRYSPRHSRSRSHISEEMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IVT1</accession>
    <name>A0A452IVT1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HMG box-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">HMG box transcription factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">High mobility group box transcription factor 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the histone H1.0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IVT1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032190"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036494.1">
        <property type="protein sequence ID" value="ENSGAGP00000032190.1"/>
        <property type="gene ID" value="ENSGAGG00000023042.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21988">
        <property type="entry name" value="HMG-box_HBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000020">
        <property type="entry name" value="HMG box-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003652">
        <property type="entry name" value="Ataxin_AXH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036096">
        <property type="entry name" value="Ataxin_AXH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039655">
        <property type="entry name" value="HBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15499">
        <property type="entry name" value="HMG BOX-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15499:SF3">
        <property type="entry name" value="HMG BOX-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08517">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00536">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102031">
        <property type="entry name" value="AXH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51148">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="AXH" evidence="16">
        <location>
            <begin position="215"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="15">
        <location>
            <begin position="448"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="13">
        <location>
            <begin position="448"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="163"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="528" mass="58951" checksum="8BB5187CD3BD45FB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWC0</accession>
    <name>A0A452IWC0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IWC0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032427"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036765.1">
        <property type="protein sequence ID" value="ENSGAGP00000032427.1"/>
        <property type="gene ID" value="ENSGAGG00000023204.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20148">
        <property type="entry name" value="PWWP_HDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000017">
        <property type="entry name" value="hepatoma-derived growth factor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047363">
        <property type="entry name" value="PWWP_HDGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550:SF41">
        <property type="entry name" value="HEPATOMA-DERIVED GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550">
        <property type="entry name" value="HEPATOMA-DERIVED GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="12"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="236" mass="26364" checksum="E0537D56D44293D2" modified="2019-05-08" version="1">MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSSSNKYQVFFFGTHETAFLGPKDLFPYEECKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQPTQKKSCPEDAEPEQEPEGDGEKKGNAEGSSDEEGKLVIDEQSKEKNEKAGIKRKAEDALEDSPKRAKEAEGQEGEKKVENEEAPKEGEKQKPSPPEGEKEKNSDPAGVPDAREAKSEGKEKGAAEEIRDHKESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYF7</accession>
    <name>A0A452IYF7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Signal peptide, CUB and EGF-like domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Forms homooligomers. Forms heterooligomers with SCUBE1 and SCUBE2. Interacts with TGFBR2 through the CUB domain; this interaction does not affect TGFB1-binding to TGFBR2. Interacts with BMP2, BMP4 and BMP7; the interaction is mediated by the CUB domain. Interacts with BMPR1A, BMPR1B and BMPR2; the interaction with BMPR1A and BMPR1B is BMP2- and BMP4-dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYF7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033086"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037506.1">
        <property type="protein sequence ID" value="ENSGAGP00000033086.1"/>
        <property type="gene ID" value="ENSGAGG00000023618.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045669">
        <property type="term" value="P:positive regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000032">
        <property type="entry name" value="signal peptide, CUB and EGF-like domain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000002">
        <property type="entry name" value="signal peptide, CUB and EGF-like domain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000006">
        <property type="entry name" value="Signal peptide, CUB domain and EGF like domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000110">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000028">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000030">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000035">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000037">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000002">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000124">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000161">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000008">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000008">
        <property type="entry name" value="Signal peptide, CUB domain, EGF-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052071">
        <property type="entry name" value="SCUB_EGF-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24046">
        <property type="entry name" value="SIGNAL PEPTIDE, CUB AND EGF-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24046:SF2">
        <property type="entry name" value="SIGNAL PEPTIDE, CUB AND EGF-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019559325" description="Signal peptide, CUB and EGF-like domain-containing protein 3" evidence="13">
        <location>
            <begin position="24"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="29"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="112"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="293"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="334"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="14">
        <location>
            <begin position="812"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1001" mass="110759" checksum="7AA32882FCA06DFD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZH6</accession>
    <name>A0A452IZH6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SOWAHA-C winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the SOWAH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZH6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033486"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037938.1">
        <property type="protein sequence ID" value="ENSGAGP00000033486.1"/>
        <property type="gene ID" value="ENSGAGG00000023859.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058889">
        <property type="entry name" value="WHD_SOWAHA-C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491:SF2">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN SOWAHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491">
        <property type="entry name" value="SOSONDOWAH, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25877">
        <property type="entry name" value="WHD_SOWAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SOWAHA-C winged helix-turn-helix" evidence="6">
        <location>
            <begin position="17"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="281"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="320"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="86"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="199"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="482" mass="52352" checksum="0A36F5BC18DE1294" modified="2019-05-08" version="1">ELESRPSSCAGASAGRAALLGFLREHGGQVRSAELRGRFKPLLEAAEPGERAARRERFTALVNSVAVVKELDGAKFVVLRKKLRAQAGGEPPEAPPEGTGRSGAGGQGRASPPLAAEESRSPKPVSELRGLFQSQDGETPGPPPGVAVPPRREAPQKPCMLPVRCPQPARGAEELPRQQEQLEDRARLQVGTLLDLPELASPRSPHMKRRQVDEAGARSPHLRRVSKTQKVSEETGCTAAVPLESLEHEWLVKATAGQWSQRLHGLLLTNANLAGKRDFMSGFTALHWAAKSGNCDMVGKIIEVAKKGGTEIDVNAKSYGGYTPLHIAAIHGQEDVITTLVKTYNVKVNLRDYSGKKPHHYLKEGSSYAVRHLLGDPDLHNSIGHAFPIKKNPKIAASILSSTSTFLGVLSDDMPFYDLSWGLKKTSSLNKLLNASAGSRKKPKTRRTFSSYSSLVEAVEEEQEEEEATVKHRPTSELFFSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J382</accession>
    <name>A0A452J382_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myosin light chain kinase, smooth muscle</fullName>
            <ecNumber evidence="5">2.7.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Telokin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.18" evidence="5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034765"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039355.1">
        <property type="protein sequence ID" value="ENSGAGP00000034765.1"/>
        <property type="gene ID" value="ENSGAGG00000024612.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004687">
        <property type="term" value="F:myosin light chain kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055013">
        <property type="term" value="P:cardiac muscle cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060298">
        <property type="term" value="P:positive regulation of sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045989">
        <property type="term" value="P:positive regulation of striated muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05762">
        <property type="entry name" value="IgI_8_hMLCK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20973">
        <property type="entry name" value="IgI_telokin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14191">
        <property type="entry name" value="STKc_MLCK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000425">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000175">
        <property type="entry name" value="Myosin light chain kinase, smooth muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000080">
        <property type="entry name" value="Myosin light chain kinase, smooth muscle"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000372">
        <property type="entry name" value="Myosin light chain kinase, smooth muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000580">
        <property type="entry name" value="Myosin light chain kinase, smooth muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000198">
        <property type="entry name" value="Myosin light chain kinase, smooth muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000107">
        <property type="entry name" value="Myosin, light chain kinase a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001127">
        <property type="entry name" value="Myosin, light chain kinase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015725">
        <property type="entry name" value="MLCK1_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF1">
        <property type="entry name" value="MYOSIN LIGHT CHAIN KINASE, SMOOTH MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16620">
        <property type="entry name" value="23ISL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="18" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="123"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="377"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="476"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="585"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="683"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="1020"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="1160"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="1256"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="1386"/>
            <end position="1641"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="1730"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="237"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="777"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="922"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="976"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1114"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1342"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="781"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="955"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="981"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1342"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="1353"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1415"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1834" mass="203519" checksum="6DE0A2C021D20CEB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J3Y4</accession>
    <name>A0A452J3Y4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Chitinase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J3Y4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035141"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039790.1">
        <property type="protein sequence ID" value="ENSGAGP00000035141.1"/>
        <property type="gene ID" value="ENSGAGG00000024979.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070492">
        <property type="term" value="F:oligosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02876">
        <property type="entry name" value="GH18_SI-CLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000002">
        <property type="entry name" value="Chitinase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000028">
        <property type="entry name" value="Chitinase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.360">
        <property type="entry name" value="3,6-anhydro-alpha-l-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46066:SF2">
        <property type="entry name" value="CHITINASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46066">
        <property type="entry name" value="CHITINASE DOMAIN-CONTAINING PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019388875" description="Chitinase domain-containing protein 1" evidence="8">
        <location>
            <begin position="20"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="9">
        <location>
            <begin position="79"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="393" mass="45067" checksum="5EF037772D1DA854" modified="2019-05-08" version="1">MRLLCTALSLTLIYQLVDATLSKTDAKKMASKTLEEKILHSDKIVQDRGLVVTDPKAKDIVLEHKSYCAKKTKERHFWGDVLGYITPWNNHGYDIAKMFGNKFTLISPVWLQVKRKGKEMFQFTGLHDADQDWIKDVKKNSKNIKIVPRILFDGWTYQDFESIFGSEDEIEELSKTMVQIAKEENFDGYVVEVWSQLGNQKQTELIHLLTHLSEALHEAQLKLILVIPPAVTPGTNQLGMFTKKEFDQLAPVMDSFSLMTYDYSTPQRPGPNSPLPWVRACVQVLDPESKWRSKILLGLNFYGMDYSALGASGEPILGNRYIETLKENKPKIVWDEQIAEHYLEYKKNKGGKHVVFYPTLKSIQLRLELAKELGTGIAIWELGQGLDYFYDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4F0</accession>
    <name>A0A452J4F0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J4F0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035312"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039986.1">
        <property type="protein sequence ID" value="ENSGAGP00000035312.1"/>
        <property type="gene ID" value="ENSGAGG00000025101.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="177"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="333" mass="38257" checksum="4CD64B2355948E2F" modified="2019-05-08" version="1">MKGDAAYSTVGSRQKQEGKLWDSMKTTGFILGTSLLLFAAFRNSVTWHLQKFWGASGDFWQAQWEKVLHLLGGNEWAIFFLGMAVVPSLVYWCFNGLLMVADVTGKPNFITRYRIQLGKNDPVDPEKLRQAVHTVLRNQVFISLPMMMFMLPIMKWRGQPCSKELPTFHWFLLELSIFILVEEILFYYSHRLVHHPLLYKRIHKQHHEWTAPVGVVSLYAHPVEHVFSNMLPLLVGPIILGSHVASIMVWLCLAILATSISHCGYHLPFLPSPEFHDFHHLKFNQCYGVLGVLDRLHGTDTLFKQTKAYERHIILLSLTPLTETIPDSPKKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J694</accession>
    <name>A0A452J694_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Secreted frizzled-related protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the secreted frizzled-related protein (sFRP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J694"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036012"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040765.1">
        <property type="protein sequence ID" value="ENSGAGP00000036012.1"/>
        <property type="gene ID" value="ENSGAGG00000025531.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000026">
        <property type="term" value="P:regulation of multicellular organismal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07443">
        <property type="entry name" value="CRD_SFRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03580">
        <property type="entry name" value="NTR_Sfrp1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.120:FF:000003">
        <property type="entry name" value="Secreted frizzled-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000001">
        <property type="entry name" value="secreted frizzled-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041760">
        <property type="entry name" value="SFRP1_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF87">
        <property type="entry name" value="SECRETED FRIZZLED-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00643">
        <property type="entry name" value="C345C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019577895" description="Secreted frizzled-related protein 1" evidence="12">
        <location>
            <begin position="32"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="13">
        <location>
            <begin position="53"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="14">
        <location>
            <begin position="186"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="68"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="314" mass="35508" checksum="DC4FA256636ACB85" modified="2019-05-08" version="1">MGRVQSPGGPSWSGAGALLALAAGLFACSHANEYDYVSFQSDIGSYQSGRFYTKPPQCVAIPADLRLCHSVGYDKMVLPNLLDHETMAEVKQQASSWVPLLNKNCHIGTQVFLCSLFAPVCLDRPIYPCRWLCEAVRDSCEPVMQFFGFYWPEMLKCDQFPQDDVCIAMTTPNATEASKPQGTAVCPPCDNEMKSDAIIEHLCASEFAFKMKIKEVKKENGDKKIVPKKKKPLKLGTIKRKDLKKLVLYLKNGADCPCHQLDNLSNHFLIMGRKVKTQYLLMAIHKWDKKNKEFKKFMKKMKTLECPTFQSLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J6K3</accession>
    <name>A0A452J6K3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J6K3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036117"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040885.1">
        <property type="protein sequence ID" value="ENSGAGP00000036117.1"/>
        <property type="gene ID" value="ENSGAGG00000025599.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19728">
        <property type="entry name" value="bHLH-PAS_HIF2a_PASD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000005">
        <property type="entry name" value="Hypoxia-inducible factor 1 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000015">
        <property type="entry name" value="Hypoxia-inducible factor 1-alpha isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000076">
        <property type="entry name" value="hypoxia-inducible factor 3-alpha isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014887">
        <property type="entry name" value="HIF-1_CTAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021537">
        <property type="entry name" value="HIF_alpha-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001067">
        <property type="entry name" value="Nuc_translocat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF8">
        <property type="entry name" value="ENDOTHELIAL PAS DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23171">
        <property type="entry name" value="bHLH_HIF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11413">
        <property type="entry name" value="HIF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08778">
        <property type="entry name" value="HIF-1a_CTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00785">
        <property type="entry name" value="NCTRNSLOCATR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="20"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="12">
        <location>
            <begin position="98"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="12">
        <location>
            <begin position="297"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="480"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="803"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="480"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="806"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-hydroxyproline" evidence="10">
        <location>
            <position position="439"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-hydroxyproline" evidence="10">
        <location>
            <position position="564"/>
        </location>
    </feature>
    <feature type="modified residue" description="(3S)-3-hydroxyasparagine" evidence="10">
        <location>
            <position position="879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR621537-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="902" mass="101683" checksum="B639A6BFDB4E8F17" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7B0</accession>
    <name>A0A452J7B0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Keratin, type I cytoskeletal 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytokeratin-14</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Keratin-14</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J7B0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036383"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041202.1">
        <property type="protein sequence ID" value="ENSGAGP00000036383.1"/>
        <property type="gene ID" value="ENSGAGG00000025790.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000002">
        <property type="entry name" value="Type I keratin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF368">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="4" id="KW-0416">Keratin</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="14">
        <location>
            <begin position="121"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="224"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="376"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="479" mass="51986" checksum="0C3944DB7EEE81DA" modified="2019-05-08" version="1">MTTSVRQYSSSTSLKGLGGLSGLGGGSTRVSSVNLGGPYRAPSIHGGSGGFSVSSSRYVSGVGSSLGGGYGGSYSSSFGGGYGGGLGGGYGGCLGGGLGGGYGVGFGGGFGGGDGILPAGEKETMQNLNDRLASYLDKVRALEEANSDLEIKIKEWYKKQGPSPDRDYSPYYRTIEDLRSKILAATIDNANIVLQIDNARLAADDFRTKFETELSLRMSVEADINGLRRVLDELTLARADLEMQIENLKEELAYLKKNHEEEMNILRSQLGGDITVEMDAAPGVDLTKILADMREQYESLAEKNRKEAEQWFFTKTEELNREVAMNTEQLQSGKSEITELRRTVQGLEIELQSQLSMKAALEGTLAETEARYGTQLAQLQALITSVEEQLAELRCDMERQNHEYKILVDVKTRLEQEIATYRRLLEGEDAHIASQYSAALSSQGGRDVISSARQVRTIVEEVQDGKVVSSREQVQLSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFN1</accession>
    <name>A0A452GFN1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">cAMP-dependent protein kinase type II-alpha regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GFN1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000371"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000416.1">
        <property type="protein sequence ID" value="ENSGAGP00000000371.1"/>
        <property type="gene ID" value="ENSGAGG00000000320.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd12103">
        <property type="entry name" value="DD_RIIalpha_PKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000017">
        <property type="entry name" value="cAMP-dependent protein kinase type II regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.890.10:FF:000002">
        <property type="entry name" value="cAMP-dependent protein kinase type II-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000027">
        <property type="entry name" value="Protein kinase cAMP-dependent type II regulatory subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF153">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE II-ALPHA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0114">cAMP</keyword>
    <keyword evidence="7 14" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="136"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="258"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="50"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="67"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="400" mass="45347" checksum="0C81F42402A561ED" modified="2019-05-08" version="1">MSIEIPPGLTELLQGYTVEVLRHRPPDLLGFAADYFTRLRDARDQEAAAAARKGVNFDGEPMQTESNGEEEPGRDDEDSDSDFEPPVINRFNRRVSVCAEAFNPDEEEEDTEPRVVHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERKVKPQEHVIDQGDDGDNFYVVERGLYDIFVTRDSQPRCVGRYDNHGSFGELALMYNTPRAATIVATTEGALWGLDRVTFRRIILKNNAKKRKTFELFIESVPLLKSLEVSERMKIVDVIGEKVYRDGERIISQGDKADSFYIVESGEVKILIKSKTMTSKDANQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNITHYEEQLVAMFGSSMDLLDPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGM0</accession>
    <name>A0A452GGM0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gremlin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DAN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GGM0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000707"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000797.1">
        <property type="protein sequence ID" value="ENSGAGP00000000707.1"/>
        <property type="gene ID" value="ENSGAGG00000000600.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036122">
        <property type="term" value="F:BMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038098">
        <property type="term" value="P:sequestering of BMP from receptor via BMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000013">
        <property type="entry name" value="Gremlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004133">
        <property type="entry name" value="DAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017159">
        <property type="entry name" value="Gremlin-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15283">
        <property type="entry name" value="GREMLIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15283:SF3">
        <property type="entry name" value="GREMLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03045">
        <property type="entry name" value="DAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037254">
        <property type="entry name" value="Gremlin_precursor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018825087" description="Gremlin" evidence="8">
        <location>
            <begin position="25"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="11">
        <location>
            <begin position="96"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="25"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="94"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="108"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="118"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="122"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR037254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="184" mass="20928" checksum="8AA2D1F70D53A241" modified="2019-05-08" version="1">MVRTVYAVGGLLLLAGILLPTAEGRKKNRGSQGAIPPPDKDQPNDSEQTQTKQQPGSRHRDRGTGTPMPAEEVLESSQEALHVTERKYLKRDWCKTQPLKQTIHEEGCNSHTIINRFCYGQCNSFYIPRHVRKEEGSFQSCSFCKPKKFTTMTVTLNCPELQPPRKKKRITRVKECRCISIDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHP6</accession>
    <name>A0A452GHP6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NAD-dependent protein deacylase sirtuin-5, mitochondrial</fullName>
            <ecNumber evidence="12">2.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Regulatory protein SIR2 homolog 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">SIR2-like protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SIRT5</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>N(6)-glutaryl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-glutaryl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11875"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87828"/>
            <dbReference type="ChEBI" id="CHEBI:87829"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-malonyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-malonyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11878"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87831"/>
            <dbReference type="ChEBI" id="CHEBI:87833"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-succinyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-succinyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11877"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87830"/>
            <dbReference type="ChEBI" id="CHEBI:87832"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
            <location evidence="2 12">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
        <text evidence="12">Mainly mitochondrial. Also present extramitochondrially, with a fraction present in the cytosol and very small amounts also detected in the nucleus.</text>
    </comment>
    <comment type="domain">
        <text evidence="12">In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the sirtuin family. Class III subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452GHP6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001097"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001226.1">
        <property type="protein sequence ID" value="ENSGAGP00000001092.1"/>
        <property type="gene ID" value="ENSGAGG00000000882.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000001232.1">
        <property type="protein sequence ID" value="ENSGAGP00000001097.1"/>
        <property type="gene ID" value="ENSGAGG00000000882.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036054">
        <property type="term" value="F:protein-malonyllysine demalonylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036055">
        <property type="term" value="F:protein-succinyllysine desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01412">
        <property type="entry name" value="SIRT5_Af1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1600.10:FF:000005">
        <property type="entry name" value="NAD-dependent protein deacylase sirtuin-5, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01121">
        <property type="entry name" value="Sirtuin_ClassIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027546">
        <property type="entry name" value="Sirtuin_class_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 12" id="KW-0520">NAD</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 12 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="14">
        <location>
            <begin position="33"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12 13">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="140"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="249"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="275"/>
            <end position="277"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="310" mass="34252" checksum="13F7960FD311BEFF" modified="2019-05-08" version="1">MSLFQFTSRRLVSQAYCGLKPPSSKKQRICLEMARPNSNMADFREVFAKAKHIAIITGAGVSAESGVPTFRGTGGYWRKWQAQELATPEAFARNSSRVWEFYHYRRELMLSKQPNPAHIAIAECEARLSKQGRSVVVITQNIDELHRKAGTKHLLEIHGSLFKTRCTSCGNVAKNYKSPICPALAGKGAPDPEIEDAKIPVENLPRCEEQGCNGLLRPHVVWFGETLNSDILTEVENELEICDLCLVVGTSSVVYPAAMFAPQVSARGVPVAEFNMQATPATNRFRFHFPGPCGTTLPPALARHKSEIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJ80</accession>
    <name>A0A452GJ80_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Aminopeptidase</fullName>
            <ecNumber evidence="18">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Release of an N-terminal amino acid, preferentially alanine, from a wide range of peptides, amides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 18">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="16 18">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A452GJ80"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001733"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001969.1">
        <property type="protein sequence ID" value="ENSGAGP00000001733.1"/>
        <property type="gene ID" value="ENSGAGG00000001389.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000001979.1">
        <property type="protein sequence ID" value="ENSGAGP00000001744.1"/>
        <property type="gene ID" value="ENSGAGG00000001389.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016285">
        <property type="term" value="F:alanyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000004">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 18" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 18" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0944">Nitration</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 18" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16 18" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="21">
        <location>
            <begin position="16"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="19">
        <location>
            <begin position="236"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="20">
        <location>
            <begin position="534"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="836" mass="94188" checksum="C9C356EBD0E3FE58" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMD4</accession>
    <name>A0A452GMD4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Microtubule associated protein 1 light chain 3 gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMD4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002996"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003430.1">
        <property type="protein sequence ID" value="ENSGAGP00000002996.1"/>
        <property type="gene ID" value="ENSGAGG00000002390.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17236">
        <property type="entry name" value="Ubl_ATG8_MAP1LC3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000149">
        <property type="entry name" value="microtubule-associated proteins 1A/1B light chain 3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027731">
        <property type="entry name" value="MAP1A/MAP1B_LC3C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019359246" description="Microtubule associated protein 1 light chain 3 gamma" evidence="11">
        <location>
            <begin position="19"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="9">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15659" checksum="8F8B6A0DEEA1CD2C" modified="2019-05-08" version="1">MCIIFVFFFYTATRLEEGAGIRAKFPTKIPVIVERYQKEKYLPLLDKTKFLVPQELTMTQFITIIRSRMALNATQAFYLLVNNKSLASMSLTLAEVYRDYKDEDGFVYMTYASQEMFGCLLPTAQGKLKEHLLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GR26</accession>
    <name>A0A452GR26_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transient receptor potential cation channel subfamily V member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Osm-9-like TRP channel 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Vanilloid receptor 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="36 37">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="37" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>Mg(2+)(in) = Mg(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29827"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendritic spine membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000004299"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005052.1">
        <property type="protein sequence ID" value="ENSGAGP00000004299.1"/>
        <property type="gene ID" value="ENSGAGG00000003572.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032591">
        <property type="term" value="C:dendritic spine membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22196">
        <property type="entry name" value="TRPV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000074">
        <property type="entry name" value="Transient receptor potential cation channel subfamily V member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000018">
        <property type="entry name" value="Transient receptor potential cation channel subfamily V member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024862">
        <property type="entry name" value="TRPV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008347">
        <property type="entry name" value="TrpV1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008348">
        <property type="entry name" value="TrpV4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00870">
        <property type="entry name" value="trp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582:SF17">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL SUBFAMILY V MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL ION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01768">
        <property type="entry name" value="TRPVRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01769">
        <property type="entry name" value="VRL2RECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 32" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="7" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="15" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="24" id="KW-0966">Cell projection</keyword>
    <keyword evidence="22" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="25" id="KW-0407">Ion channel</keyword>
    <keyword evidence="20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="21 34" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="19" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0770">Synapse</keyword>
    <keyword evidence="9 34" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 34" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="484"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="523"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="549"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="589"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="669"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="32">
        <location>
            <begin position="212"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="35">
        <location>
            <begin position="445"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="33">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="33">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00031766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00033256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00034269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="822" mass="94278" checksum="E3481EB5ACC7FBEE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRZ3</accession>
    <name>A0A452GRZ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Complex III assembly factor LYRM7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">LYR motif-containing protein 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Assembly factor required for Rieske Fe-S protein UQCRFS1 incorporation into the cytochrome b-c1 (CIII) complex. Functions as a chaperone, binding to this subunit within the mitochondrial matrix and stabilizing it prior to its translocation and insertion into the late CIII dimeric intermediate within the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with UQCRFS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GRZ3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004757"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005571.1">
        <property type="protein sequence ID" value="ENSGAGP00000004757.1"/>
        <property type="gene ID" value="ENSGAGG00000003911.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20267">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045298">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050435">
        <property type="entry name" value="MZM1/LYRM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749:SF1">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="10">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="104" mass="11966" checksum="0A9E5D14964FB8E6" modified="2019-05-08" version="1">MGSRAEVLKLFRALHRTRQQVFKNDARALEAARQKINEEFRNHQDETSSERIAELMKIGSDVEFLLRTSVIQGLHTGSDSLMLIPRKDLLLDNVPYCDSPTRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GT84</accession>
    <name>A0A452GT84_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein P2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">60S acidic ribosomal protein P2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the elongation step of protein synthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GT84"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004929"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005756.1">
        <property type="protein sequence ID" value="ENSGAGP00000004929.1"/>
        <property type="gene ID" value="ENSGAGG00000004036.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05833">
        <property type="entry name" value="Ribosomal_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="80"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="113" mass="11664" checksum="9EE093FF9135EC4A" modified="2019-05-08" version="1">MRYVAAYLLAVLGGNESPASKDLKKILDSVGIETDDERLNKVIGELNGKNIEDVIAQGNGKLASMPVGGAVAVSAGPASAAPAAAPAAAEEKKEEKKEESEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUM1</accession>
    <name>A0A452GUM1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GUM1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005792"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006755.1">
        <property type="protein sequence ID" value="ENSGAGP00000005792.1"/>
        <property type="gene ID" value="ENSGAGG00000004712.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002521">
        <property type="term" value="P:leukocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21995">
        <property type="entry name" value="HMG-box_TOX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000005">
        <property type="entry name" value="TOX high mobility group box family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051365">
        <property type="entry name" value="TOX_HMG-box_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45781">
        <property type="entry name" value="AGAP000281-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45781:SF4">
        <property type="entry name" value="THYMOCYTE SELECTION-ASSOCIATED HIGH MOBILITY GROUP BOX PROTEIN TOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="240"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="240"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="171"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="171"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="505" mass="55230" checksum="FB4F9E79D7B958C4" modified="2019-05-08" version="1">MDVRFYSPPPPPQPAATPDTPCLGPSPCLDPYYCNKSFPGPSLESEDFNIPPITPPSLPDHSLVHLNEVESGYHSLCHPMNHNGLLPFHPQNMDLPEITVSNMLGQDGTLLSNSLSVMQDIRNTEGAQYSSHPQMAAMRPRVQPAEIRQPGMMQHGQLTTINQSQLSAQLGLNMGGNNVPHNSPSPPGSKSATPSPSSSVHEDEGDDTSKVNGGEKRPASDMGKKPKTPKKKKKKDPNEPQKPVSAYALFFRDTQAAIKGQNPNATFGEVSKIVASMWDGLGEEQKQVYKKKTEAAKKEYLKQLAAYRASLVSKSYSEPVDVKASQPPQMINSKQSVFHGPTQAHSALYLSSHYHQQSGINPHITAMHANIPRNIAPKPNNQMPVTVSIANMAVSPPPPLQISPPLHQHLNIQQHQPITMQQSIGNQLPMQVQSALHSPTMQQGFTLQPDYQNIINPTSTAAQVVTQAMEYVRSGCRNPPAQTVDWNNDYCSNGGMQRDKALYLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUY7</accession>
    <name>A0A452GUY7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GUY7"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006918.1">
        <property type="protein sequence ID" value="ENSGAGP00000005933.1"/>
        <property type="gene ID" value="ENSGAGG00000004823.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070652">
        <property type="term" value="C:HAUS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029327">
        <property type="entry name" value="HAUS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026214">
        <property type="entry name" value="HAUS4_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16219">
        <property type="entry name" value="AUGMIN SUBUNIT 4 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16219:SF1">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02090">
        <property type="entry name" value="HAUSAUGMINL4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="290" mass="31275" checksum="CD7D47991DE95483" modified="2019-05-08" version="1">WLPRPRRWETWAWSCWDQVSGGGVRLGGGCPSLPLTVSPHSCWCPPAPVPVDRGGCECPSWLGQPAAWPDPLSGPQWAQHPPGPPAGAGTCGDAPREGGCPAAACSPPSHLLQVGKELQLRRAAWLRWEALHRLLQEALIEQRAGRGDASPALEHRKFLETLEQRLLLGELSRILEPGPSLRGDRPPLLGLGAPDLRELLPPSQVTQPPWTRSGRDPGVRALSPLSVWPPPVQDLALLQQHLPQEIEERLRRKGLALLSYHRPDSGEWVGAGEQGGGSGEGVSSDPCAPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVM0</accession>
    <name>A0A452GVM0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Corticotropin-releasing factor-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Corticotropin-releasing hormone-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRF-binding protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GVM0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006077"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007083.1">
        <property type="protein sequence ID" value="ENSGAGP00000006077.1"/>
        <property type="gene ID" value="ENSGAGG00000004937.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000007086.1">
        <property type="protein sequence ID" value="ENSGAGP00000006080.1"/>
        <property type="gene ID" value="ENSGAGG00000004937.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051424">
        <property type="term" value="F:corticotropin-releasing hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051460">
        <property type="term" value="P:negative regulation of corticotropin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008435">
        <property type="entry name" value="CRF-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056178">
        <property type="entry name" value="CRF-BP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056177">
        <property type="entry name" value="CRF-BP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10278">
        <property type="entry name" value="CORTICOTROPIN-RELEASING FACTOR-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10278:SF0">
        <property type="entry name" value="CORTICOTROPIN-RELEASING FACTOR-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23541">
        <property type="entry name" value="CRF-BP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05428">
        <property type="entry name" value="CRF-BP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009279">
        <property type="entry name" value="CRF_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604494" description="Corticotropin-releasing factor-binding protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Corticotropin-releasing factor binding protein N-terminal" evidence="11">
        <location>
            <begin position="52"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Corticotropin-releasing factor binding protein C-terminal" evidence="12">
        <location>
            <begin position="185"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="282" mass="31552" checksum="92FE553A58BDDCFD" modified="2019-05-08" version="1">MASAFRLQCHCILIFLAALKGETRYLEAKGAGEDEPFLLLSADLKRELAGRQLYHRALRCIDMLSVEGQFTFTAEQPQLHCAAFFIGDPEELITLDYDFVNIDCQGGDFLKVFDGWILKGEKFPSSLDHPLPTSERYIDFCESGNIRRSFRSSQNVAMIFFRIHEPGNGFTFTIKKNANLFPCNVISQTPTGRFTMVIPHQHRNCSFSIIYPVVIKISDLILGHLNSLHLKKPSTGCAGVGDFVELLGGTGLDPSKMFPLADLCQSFHGSGEIFCLNLCMRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWR5</accession>
    <name>A0A452GWR5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GWR5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006464"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007521.1">
        <property type="protein sequence ID" value="ENSGAGP00000006464.1"/>
        <property type="gene ID" value="ENSGAGG00000005231.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="67" mass="7510" checksum="4ADEE71BF381BD73" modified="2019-05-08" version="1">SLLNSRGLILIFSLSKPDMISQLEQGEEPWVLDLQGLEEREILTGACTDEGSLNQYGQLMQIMNIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GX59</accession>
    <name>A0A452GX59_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Polyamine-transporting ATPase 13A2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">ATPase which acts as a lysosomal polyamine exporter with high affinity for spermine. Also stimulates cellular uptake of polyamines and protects against polyamine toxicity. Plays a role in intracellular cation homeostasis and the maintenance of neuronal integrity. Contributes to cellular zinc homeostasis. Confers cellular protection against Mn(2+) and Zn(2+) toxicity and mitochondrial stress. Required for proper lysosomal and mitochondrial maintenance. Regulates the autophagy-lysosome pathway through the control of SYT11 expression at both transcriptional and post-translational levels. Facilitates recruitment of deacetylase HDAC6 to lysosomes to deacetylate CTTN, leading to actin polymerization, promotion of autophagosome-lysosome fusion and completion of autophagy. Promotes secretion of exosomes as well as secretion of SCNA via exosomes. Plays a role in lipid homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>spermidine(out) + ATP + H2O = spermidine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29999"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>spermine(out) + ATP + H2O = spermine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63368"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:45725"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="22">Interacts with MYCBP2; the interaction inhibits the ubiquitination of TSC2 by MYCBP2. Interacts with HDAC6; the interaction results in recruitment of HDAC6 to lysosomes to promote CTTN deacetylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Autophagosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Endosome</location>
            <location evidence="21">Multivesicular body membrane</location>
            <topology evidence="21">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GX59"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006689"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007767.1">
        <property type="protein sequence ID" value="ENSGAGP00000006689.1"/>
        <property type="gene ID" value="ENSGAGG00000004981.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015417">
        <property type="term" value="F:ABC-type polyamine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061909">
        <property type="term" value="P:autophagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903543">
        <property type="term" value="P:positive regulation of exosomal secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061462">
        <property type="term" value="P:protein localization to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016243">
        <property type="term" value="P:regulation of autophagosome size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905165">
        <property type="term" value="P:regulation of lysosomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010821">
        <property type="term" value="P:regulation of mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900180">
        <property type="term" value="P:regulation of protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07542">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000023">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000060">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000026">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000045">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047821">
        <property type="entry name" value="P5B-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF2">
        <property type="entry name" value="POLYAMINE-TRANSPORTING ATPASE 13A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="810"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="835"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="873"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="27">
        <location>
            <begin position="96"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="26">
        <location>
            <begin position="179"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1039"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="24">
        <location>
            <begin position="1042"/>
            <end position="1052"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00053898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00060400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00065284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00074227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1102" mass="121007" checksum="5258DFFDBF6FC7CA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXR5</accession>
    <name>A0A452GXR5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">E3 ubiquitin-protein ligase FANCL</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">RING-type E3 ubiquitin transferase FANCL</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452GXR5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006902"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008004.1">
        <property type="protein sequence ID" value="ENSGAGP00000006902.1"/>
        <property type="gene ID" value="ENSGAGG00000005562.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23832">
        <property type="entry name" value="DRWD-C_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23831">
        <property type="entry name" value="DRWD-N_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16490">
        <property type="entry name" value="RING-CH-C4HC3_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000081">
        <property type="entry name" value="E3 ubiquitin-protein ligase FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.20:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000221">
        <property type="entry name" value="E3 ubiquitin-protein ligase FANCL isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.20">
        <property type="entry name" value="RWD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026848">
        <property type="entry name" value="Fancl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026850">
        <property type="entry name" value="FANCL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043898">
        <property type="entry name" value="FANCL_d2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044037">
        <property type="entry name" value="FANCL_d3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043003">
        <property type="entry name" value="FANCL_d3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13206:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13206">
        <property type="entry name" value="UBIQUITIN LIGASE PROTEIN PHF9 FANCONI ANEMIA GROUP L PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11793">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18890">
        <property type="entry name" value="FANCL_d2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18891">
        <property type="entry name" value="FANCL_d3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="245"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00073910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="313" mass="35209" checksum="5FD3979E4FE2FA9A" modified="2019-05-08" version="1">MRVTRAEAGFAAARGIRFEEEPAPPARARGGRWRVPGRFCASARCSCLRTARGASTRGSSPCRVVYVDTGLNTIKLKGEDSCGRQHLITLKLNAKYPAEPPDCCVDFPVEFAISWIPQSSLVDIYNQFLAALESLKEFWNAMDEIDEKTWVLEPENPTRSATRRRIAIGNNVSINLEVDPRHPTMLPECYFLGADHVVNPLRTKLNNNMHLWDPDITLLQNLKEVLETDFPSRAVLEKTDFTMDCGICYAYRLDGAIPDQVCGDSRCGQPFHQACLFEWLQGLPSSRQSFNVIFGECPYCNKPISVKMSMKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GY07</accession>
    <name>A0A452GY07_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Neurexophilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May be signaling molecules that resemble neuropeptides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the neurexophilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GY07"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006999"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008109.1">
        <property type="protein sequence ID" value="ENSGAGP00000006999.1"/>
        <property type="gene ID" value="ENSGAGG00000005638.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010450">
        <property type="entry name" value="Nxph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026845">
        <property type="entry name" value="NXPH/NXPE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17103">
        <property type="entry name" value="NEUREXOPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17103:SF13">
        <property type="entry name" value="NEUREXOPHILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06312">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038019">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019435803" description="Neurexophilin" evidence="5">
        <location>
            <begin position="22"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="271" mass="31062" checksum="B16950405EF0122C" modified="2019-05-08" version="1">MQAVYWYAVLLLQPTLYLVTCANLTNGGKSELLKSGNSKSTLKHIWTESSKDLSISRLLSQTFHGKENDTDLDLQYDAPETYSEQDLWDWLRNSTDLQEPRPRAKRRPIVKTGKFKKMFGWGDFHSNIKTVKLNLLITGKIVDHGNGTFSVYFRHNSTGQGNVSVSLVPPTKIVEFDLAQQTVIDAKDSKSFNCRIEYEKVDKATKNTLCNYDPSKTCYQEQTQSHVSWLCSKPFKVICIYISFYSTDYKLVQKVCPDYNYHSDTPYFPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYN1</accession>
    <name>A0A452GYN1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GYN1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007340"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008484.1">
        <property type="protein sequence ID" value="ENSGAGP00000007340.1"/>
        <property type="gene ID" value="ENSGAGG00000005895.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF163">
        <property type="entry name" value="ZINC FINGER PROTEIN 589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="3"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="122" mass="13898" checksum="768340A66F5DF6DD" modified="2019-05-08" version="1">MELPLFEVAVYFTREEGTLLDPTQRALYRDVMQQNYENVTSLAGFLVFQHDVISQLEQGEEPWVPDLQGSEEREILRSSCTDEKTLNQLRICKCLKETSGVPNKALGEFPPFPLGIWMGCEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYR4</accession>
    <name>A0A452GYR4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GYR4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007389"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008537.1">
        <property type="protein sequence ID" value="ENSGAGP00000007389.1"/>
        <property type="gene ID" value="ENSGAGG00000005932.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="186" mass="20761" checksum="50304E1AC11AB761" modified="2019-05-08" version="1">MTSEGPGARKDPRLGRQKPWRRSWDAPTSDPRVRLLGERVLRSLRLKPEHWERCAGSPEVQPLLWGSVESAAGQPPLLLVTLSPAGQLALSTEFPASPGRGKALFFLELLYGDLPASSLEHFAALVEEVRGPRLLRDNNAKLEHTQHSVTPCILVHQPHTSLFPDTIHSQRKAALLIHIHRPPRYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZY7</accession>
    <name>A0A452GZY7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZY7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007849"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009048.1">
        <property type="protein sequence ID" value="ENSGAGP00000007849.1"/>
        <property type="gene ID" value="ENSGAGG00000006251.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.930:FF:000004">
        <property type="entry name" value="Integrin subunit alpha M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000012">
        <property type="entry name" value="Integrin, alpha 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048633">
        <property type="entry name" value="ITGAX-like_Ig_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF118">
        <property type="entry name" value="INTEGRIN ALPHA-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00357">
        <property type="entry name" value="Integrin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21520">
        <property type="entry name" value="ITGAX-like_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 16" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0401">Integrin</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="935"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="18">
        <location>
            <begin position="85"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="324"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="379"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="440"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="499"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="973"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="982"/>
            <end position="993"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="999" mass="109889" checksum="CC456CDA7840B3FC" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H020</accession>
    <name>A0A452H020_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452H020"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007791"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008980.1">
        <property type="protein sequence ID" value="ENSGAGP00000007791.1"/>
        <property type="gene ID" value="ENSGAGG00000006207.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13195">
        <property type="entry name" value="FERM_C_MYLIP_IDOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17104">
        <property type="entry name" value="FERM_F1_MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16523">
        <property type="entry name" value="RING-HC_MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000019">
        <property type="entry name" value="E3 ubiquitin-protein ligase MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000129">
        <property type="entry name" value="E3 ubiquitin-protein ligase MYLIP isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000164">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000175">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041790">
        <property type="entry name" value="MYLIP_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF13">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FERM" evidence="12">
        <location>
            <begin position="6"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="392"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="450" mass="50513" checksum="D4B5C2B78364B515" modified="2019-05-08" version="1">MSAPAMLCYVTRPDAVVMEVEVEAKANGEDCLNQVCRRLGIIEVDYFGLQFTGSKGESLWLNLRNRISQQMDGLAPYRLKLRVKFFVEPHLILQEQTRHMFFLHIREDLLAGNLQCSSEHAIELSALLAQMKFGDYNQNTAKYSYEELCAKELTTATLDSITAKHKELEGLSQASAEYQVLQIVSTLENYGVEWHSVRDSEGQKLLIGVGPEGISICKDDFSPINRIAYPVVQMATQSGKNVYLTVTNESGNSVVLLFKMISTRAASGLYRAITETHAFYRCDTVTSAVMMQYSRDLKGHLASLFLNENINLGKKYVFDIKRTSKEVYDHARRTLYNAGIVDLVSRSDQTPPNSPLKSSESNMNCDSCEGLSCQQTKALQEKLRKLKESMLCMVCCEEEINSTFCPCGHTVCCETCAAQLQSCPVCRSRVEHVQHVYLPTHTSLLNLTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0K6</accession>
    <name>A0A452H0K6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc finger protein RFP-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000007971"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009187.1">
        <property type="protein sequence ID" value="ENSGAGP00000007971.1"/>
        <property type="gene ID" value="ENSGAGG00000006346.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19762">
        <property type="entry name" value="Bbox2_TRIM7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16594">
        <property type="entry name" value="RING-HC_TRIM7-like_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="93"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="237"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="168"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="45549" checksum="E95674AAE15C2947" modified="2019-05-08" version="1">MEKSPLESLQEEATCPVCLEYFTEPVTLECGHNFCRACIAQCWEGSDTAASCPQCRETVQQRNLRPNRQLANILEIAKRLSLQAAKGAGGDGVCGEHQEALKLFCEEDQSPICVICRESRAHRTHTVVPIQEAAQEYKVPSSCSRSQEYLKQTETERQKIVSEFQQLHQFLEEQERLLLAQLKKLDEEILKIQNENITKLSEEISCLSELISEMEGKCQKPASEFLQVRLNWKHLESQHRDRTIPELGAQGSMTVTVTLDPETAHPKLILSEDRKHVRYGDIWQQLPSNPERFDTALCVLGCEGFTSGRHCWEVEVGDGGRWAVGVARESVRRKXGRKGWISRSPEGGIWAVGWRWGGQFRVGMHYKYGSRKSVHLHTHREYCPVQLLATGFWLKPPAALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H130</accession>
    <name>A0A452H130_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H130"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008163"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009400.1">
        <property type="protein sequence ID" value="ENSGAGP00000008163.1"/>
        <property type="gene ID" value="ENSGAGG00000006483.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060340">
        <property type="term" value="P:positive regulation of type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19808">
        <property type="entry name" value="Bbox1_TRIM42_C-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF299">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="154"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="6">
        <location>
            <begin position="229"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="8">
        <location>
            <begin position="299"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="616"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="731" mass="84231" checksum="6F51F18B2A43EBFB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H215</accession>
    <name>A0A452H215_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1</fullName>
            <ecNumber evidence="5">3.1.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">HD domain-containing protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Metazoan SpoT homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Penta-phosphate guanosine-3'-pyrophosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">ppGpp hydrolyzing enzyme involved in starvation response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>guanosine 3',5'-bis(diphosphate) + H2O = GDP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14253"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:77828"/>
            <dbReference type="EC" id="3.1.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MESH1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.7.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452H215"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008445"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009721.1">
        <property type="protein sequence ID" value="ENSGAGP00000008445.1"/>
        <property type="gene ID" value="ENSGAGG00000006713.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008893">
        <property type="term" value="F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000012">
        <property type="entry name" value="HD domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052194">
        <property type="entry name" value="MESH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46246">
        <property type="entry name" value="GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46246:SF1">
        <property type="entry name" value="GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13328">
        <property type="entry name" value="HD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="13">
        <location>
            <begin position="46"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="193" mass="21684" checksum="A186A3ADC91F5135" modified="2019-05-08" version="1">MHEVHGSWARSSPSWNWAAVLTGLCQREAPGPPPDGLSGAWLPADTPTPCLGVARILSQEAGVMDIAVLQAALLHDTVEDTDTTFSEIEEQFGEEVRRIVEEVTDDKMLPKMERKRLQIERAPHGSRGAKLVKLADKLYNLRDLNRCTPAGWSEQRVQEYFLWASQVVKGLRETSPALEEKLQQLFRERGLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2H9</accession>
    <name>A0A452H2H9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Neuroendocrine protein 7B2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 7B2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H2H9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008788"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010101.1">
        <property type="protein sequence ID" value="ENSGAGP00000008788.1"/>
        <property type="gene ID" value="ENSGAGG00000006951.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046883">
        <property type="term" value="P:regulation of hormone secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007945">
        <property type="entry name" value="Secretogranin_V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12738">
        <property type="entry name" value="NEUROENDOCRINE PROTEIN 7B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12738:SF0">
        <property type="entry name" value="NEUROENDOCRINE PROTEIN 7B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05281">
        <property type="entry name" value="Secretogranin_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0765">Sulfation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019067933" description="Neuroendocrine protein 7B2" evidence="10">
        <location>
            <begin position="25"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="212" mass="23582" checksum="8E88A396EDB07D25" modified="2019-05-08" version="1">MAIRMTTTLLYNMVFLLAFGLALASSHSPRTPDKVSEADIQRLLHGVMEQLGIARPRVEYPAHQAMNLVGPQSIEGGAHEGLQHLGPYGNIPNIVAELTGDNIPKDFSEDQGYPDPPNPCPIGKTADDGCLQNTPDTAEFSREYQLHQHLFDPEHDYPSLGKWNKNFLFEKIKGGPKRRKRSVNPYLQGQRLDNVVAKKSVPHFSDEDKGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H355</accession>
    <name>A0A452H355_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Synphilin-1 alpha-Synuclein-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H355"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009047"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010385.1">
        <property type="protein sequence ID" value="ENSGAGP00000009047.1"/>
        <property type="gene ID" value="ENSGAGG00000007143.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040133">
        <property type="entry name" value="SNCAIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032027">
        <property type="entry name" value="SNCAIP_SNCA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22882">
        <property type="entry name" value="SYNPHILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22882:SF3">
        <property type="entry name" value="SYNPHILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16700">
        <property type="entry name" value="SNCAIP_SNCA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="454"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Synphilin-1 alpha-Synuclein-binding" evidence="4">
        <location>
            <begin position="510"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="547"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="671"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="733"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="823"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="889"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="520"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="554"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="672"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="684"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="734"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="755"/>
            <end position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="958" mass="105360" checksum="204FDA3074107922" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H387</accession>
    <name>A0A452H387_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H387"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009079"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010420.1">
        <property type="protein sequence ID" value="ENSGAGP00000009079.1"/>
        <property type="gene ID" value="ENSGAGG00000007172.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000010423.1">
        <property type="protein sequence ID" value="ENSGAGP00000009082.1"/>
        <property type="gene ID" value="ENSGAGG00000007172.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000002">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="13">
        <location>
            <begin position="49"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="14">
        <location>
            <begin position="248"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="449" mass="49894" checksum="B9F8554E5DF9C907" modified="2019-05-08" version="1">MRECISVHVGQAGVQIGNACWELYCLEHGIQPNGTMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPAVIDEVRNGTYKQLFHPEQLISGKEDAANNYARGHYTVGKEIIDLVLERIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYSPIISAEKAYHEQLSVSEITNACFEPSNQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIAAIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGTDSIDGEDEGEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3W5</accession>
    <name>A0A452H3W5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="23">Cell junction</location>
            <location evidence="23">Hemidesmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 24">Cell membrane</location>
            <topology evidence="2 24">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 24">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000009305"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010689.1">
        <property type="protein sequence ID" value="ENSGAGP00000009305.1"/>
        <property type="gene ID" value="ENSGAGG00000006910.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030056">
        <property type="term" value="C:hemidesmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000212">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000215">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000287">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000424">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2030:FF:000004">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000036">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.30:FF:000003">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003644">
        <property type="entry name" value="Calx_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040622">
        <property type="entry name" value="EGF_integrin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057073">
        <property type="entry name" value="EGF_integrin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033760">
        <property type="entry name" value="Integrin_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012896">
        <property type="entry name" value="Integrin_bsu_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036349">
        <property type="entry name" value="Integrin_bsu_tail_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002369">
        <property type="entry name" value="Integrin_bsu_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057243">
        <property type="entry name" value="Integrin_I-EGF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF42">
        <property type="entry name" value="INTEGRIN BETA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03160">
        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23105">
        <property type="entry name" value="EGF_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18372">
        <property type="entry name" value="I-EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07965">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00362">
        <property type="entry name" value="Integrin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17205">
        <property type="entry name" value="PSI_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00237">
        <property type="entry name" value="Calx_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00187">
        <property type="entry name" value="INB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01242">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69687">
        <property type="entry name" value="Integrin beta tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00243">
        <property type="entry name" value="I_EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52047">
        <property type="entry name" value="I_EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 24" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="14" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 24" id="KW-0401">Integrin</keyword>
    <keyword evidence="22" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 27" id="KW-0732">Signal</keyword>
    <keyword evidence="7 24 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019175707" description="Integrin beta" evidence="27">
        <location>
            <begin position="25"/>
            <end position="1713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="703"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="28">
        <location>
            <begin position="1117"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="28">
        <location>
            <begin position="1210"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="28">
        <location>
            <begin position="1501"/>
            <end position="1630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1396"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1400"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="1416"/>
            <end position="1429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00060371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1713" mass="191648" checksum="B3B2759AB016831D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4H1</accession>
    <name>A0A452H4H1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">REM-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000009508"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010924.1">
        <property type="protein sequence ID" value="ENSGAGP00000009508.1"/>
        <property type="gene ID" value="ENSGAGG00000007522.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11630">
        <property type="entry name" value="HR1_PKN1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11622">
        <property type="entry name" value="HR1_PKN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000002">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000003">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.160">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037317">
        <property type="entry name" value="PKN1_HR1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037313">
        <property type="entry name" value="PKN_HR1_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="REM-1" evidence="4">
        <location>
            <begin position="35"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="4">
        <location>
            <begin position="119"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="17"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="310" mass="34321" checksum="9A78292AF899C475" modified="2019-05-08" version="1">PTARVARGEQLLRQLLTEGQKRGRSCPPPPPCSGSLPSSDSVQQRLDEEKERIRREIRKELKIKEGAENLRRATTDRRNLASVESLLRGSDRKLQQLHCRLQHLDAHIMVKEPEESGDAPQSPNSLSRSAANKHRLAGLEKQLNIELKVKQGAENMIQMYANGSTKDRKLLQTAQQMLQDSKTKINIIRMQIRRAAQADVVPPEGEDVQGERGSGGRGNRLGSHGAWGSWGAVSYTHLDVYKRQPPPFSXPAVDTHPAQTPTILCSPWPRSLDRCGSGGGTWWADPGALSTWEVFIQQTHPLVSRGPATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HB87</accession>
    <name>A0A452HB87_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HB87"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012024"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013768.1">
        <property type="protein sequence ID" value="ENSGAGP00000012024.1"/>
        <property type="gene ID" value="ENSGAGG00000009261.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF142">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="17"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="155" mass="16903" checksum="2F5E306B1F96C87C" modified="2019-05-08" version="1">DRKRRLLPGPGPGPGPVTFEEVAVYFTREEGALLDPTRRALYRDVMRENYETVTSLGFPVSKPSVISQLEGGEEPWILDLQGSEESKILRSPWTGEEPSNQPRNYKCLRDPAGITHTDSVSSGQSPADLTPGFLQMVIDISAGAASLPTECLDGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCJ5</accession>
    <name>A0A452HCJ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Adenosine deaminase</fullName>
            <ecNumber evidence="7">3.5.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Adenosine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>2'-deoxyadenosine + H2O + H(+) = 2'-deoxyinosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:28190"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17256"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:28997"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:28191"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>adenosine + H2O + H(+) = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:24409"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.4" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452HCJ5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012523"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014349.1">
        <property type="protein sequence ID" value="ENSGAGP00000012523.1"/>
        <property type="gene ID" value="ENSGAGG00000009615.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000014351.1">
        <property type="protein sequence ID" value="ENSGAGP00000012525.1"/>
        <property type="gene ID" value="ENSGAGG00000009615.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060205">
        <property type="term" value="C:cytoplasmic vesicle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043103">
        <property type="term" value="P:hypoxanthine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060169">
        <property type="term" value="P:negative regulation of adenosine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009168">
        <property type="term" value="P:purine ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042110">
        <property type="term" value="P:T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000038">
        <property type="entry name" value="Adenosine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01430">
        <property type="entry name" value="aden_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF43">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0458">Lysosome</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Adenosine deaminase" evidence="22">
        <location>
            <begin position="20"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00018099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="366" mass="41518" checksum="45A5204267F52455" modified="2019-05-08" version="1">MISVSGSLSMFDSLQSLLLVELHVHLDGAIKLETILHFGKKRGIPLPGDTVEDLLELISYSEPLTLTQFLEKFALYMPAIAGDRDAVRRIAYEFVEMKAKEGVIYVEVRYSPHLLANCKVHPVPWDQEEGDLTPDEVVHLVNQGLQDGERDFNIKARSILCCMRHMPSWSLEVVELCKKYQNNTVVAIDLAGDELVSETFPEHRKAYEEAERCGIHRTVHAGEVGPPSVIKEAINVLKTERIGHGYHVLEDPALYSELLSKKMHFETCPWSSILTGACDPDFRKHPLIQFRKDKANYSLNTDDPLVFNSSIETDYRIAKQSLDFTEEEFKRVNINAAQSSFLPEKEKKELLSKLYEAYGMVPNNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDY8</accession>
    <name>A0A452HDY8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HDY8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014924.1">
        <property type="protein sequence ID" value="ENSGAGP00000013027.1"/>
        <property type="gene ID" value="ENSGAGG00000010000.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.75.10">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036645">
        <property type="entry name" value="Elafin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008197">
        <property type="entry name" value="WAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00095">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00217">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57256">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51390">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019286042" description="WAP domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="3">
        <location>
            <begin position="24"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="101" mass="10792" checksum="08AFA95CABF5C515" modified="2019-05-08" version="1">MRSCIVIVLHGTDVVTWALVSCTATAKDGECPAEMKGPTRPPQAYCLSDESCPGNEKCCSQGDRRVCILPDSGTGESAALSDRAENRGFAHHLKSETRGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFQ1</accession>
    <name>A0A452HFQ1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA cross-link repair 1A protein</fullName>
            <ecNumber evidence="4">3.5.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">SNM1 homolog A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a beta-lactam + H2O = a substituted beta-amino acid</text>
            <dbReference type="Rhea" id="RHEA:20401"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35627"/>
            <dbReference type="ChEBI" id="CHEBI:140347"/>
            <dbReference type="EC" id="3.5.2.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.6" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452HFQ1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013633"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015604.1">
        <property type="protein sequence ID" value="ENSGAGP00000013633.1"/>
        <property type="gene ID" value="ENSGAGG00000010394.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12650:FF:000001">
        <property type="entry name" value="DNA cross-link repair 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000010">
        <property type="entry name" value="DNA cross-link repair 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF6">
        <property type="entry name" value="DNA CROSS-LINK REPAIR 1A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="11">
        <location>
            <begin position="407"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="109"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="530" mass="59631" checksum="FB93CCC131210260" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGK6</accession>
    <name>A0A452HGK6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HGK6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013993"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016014.1">
        <property type="protein sequence ID" value="ENSGAGP00000013993.1"/>
        <property type="gene ID" value="ENSGAGG00000010654.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008046">
        <property type="term" value="F:axon guidance receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000022">
        <property type="entry name" value="Cardiac titin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050958">
        <property type="entry name" value="Cell_Adh-Cytoskel_Orgn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45080">
        <property type="entry name" value="CONTACTIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45080:SF8">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="108"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="255" mass="28319" checksum="A324B9E8205DAAE7" modified="2019-05-08" version="1">MLKGTDVSLECEISGTPPFEVMWYKDKRQIRSSKKYKVTSKNYHASIHILNVDASDIGEYQCKAQNEVGSEFICTESVSGPLFFGKCSCLLNRCGFVTFSYTDRIIPPSFTRKLKETNGVLGSSALLECKVSGSPPISIAWFQDGKKIISGVKHQTTFSDNLCVLQLNSLTSSDTGSYSCKATNVAGSDECRAVLNVQGQYLTANQLFAVNPLLYNSLFHLVFQAVMSESVNFLYCICKRVNCSLNSSWHYTCFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGS5</accession>
    <name>A0A452HGS5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Interferon alpha inducible protein 27 like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFI6/IFI27 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGS5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014053"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016092.1">
        <property type="protein sequence ID" value="ENSGAGP00000014053.1"/>
        <property type="gene ID" value="ENSGAGG00000010706.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000016099.1">
        <property type="protein sequence ID" value="ENSGAGP00000014060.1"/>
        <property type="gene ID" value="ENSGAGG00000010706.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097193">
        <property type="term" value="P:intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009311">
        <property type="entry name" value="IFI6/IFI27-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038213">
        <property type="entry name" value="IFI6/IFI27-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16932">
        <property type="entry name" value="INTERFERON ALPHA-INDUCIBLE PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16932:SF18">
        <property type="entry name" value="INTERFERON, ALPHA-INDUCIBLE PROTEIN 27-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06140">
        <property type="entry name" value="Ifi-6-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="12931" checksum="B5C54F0D6FFFA726" modified="2019-05-08" version="1">MLDWSIPKSFPSYSAILTLAEARHGLPGLSCSLLASEMLLTETGKPSDSLVTLAGAGIAAGVAFIGIPVVVGAAGFTGAGIAAGTLAAKMMSAAAIANGGGVAAGSTVAVLQSIGAAGLPAAAKAVIVSVGAAAGALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJ49</accession>
    <name>A0A452HJ49_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BORG/CEP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJ49"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014932"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017057.1">
        <property type="protein sequence ID" value="ENSGAGP00000014932.1"/>
        <property type="gene ID" value="ENSGAGG00000011287.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031274">
        <property type="term" value="P:positive regulation of pseudopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029273">
        <property type="entry name" value="Cdc42_effect-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051296">
        <property type="entry name" value="Cdc42_Effector_BORG/CEP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15344:SF3">
        <property type="entry name" value="CDC42 EFFECTOR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15344">
        <property type="entry name" value="CDC42 EFFECTOR PROTEIN BORG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14957">
        <property type="entry name" value="BORG_CEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="3">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="256" mass="28106" checksum="CF1B89DEB807E76E" modified="2019-05-08" version="1">MPAKTPIYLKAANTKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQGNYELLPGNEGTPRVGQFGGHNEFLRANSTCDSMFTETPSPVLKNAISLPAIGGSQALVLPLLSPVTFNSKRDSFGPSKNPRLSCEPVMEEKLQEKSKQLENGEMYKDDILWEPNGSGSHYTNGRESHSSSLSEQCTDWQTVDLLDDSHLSCELTKAETKSEESLSDLAGSLLSLQLDLGPSLLDEVLNVMDKNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HK94</accession>
    <name>A0A452HK94_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CELF/BRUNOL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HK94"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015337"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017516.1">
        <property type="protein sequence ID" value="ENSGAGP00000015337.1"/>
        <property type="gene ID" value="ENSGAGG00000011562.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12631">
        <property type="entry name" value="RRM1_CELF1_2_Bruno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12634">
        <property type="entry name" value="RRM2_CELF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12638">
        <property type="entry name" value="RRM3_CELF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000013">
        <property type="entry name" value="CUGBP Elav-like family member 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000015">
        <property type="entry name" value="CUGBP Elav-like family member 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000016">
        <property type="entry name" value="CUGBP Elav-like family member 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034196">
        <property type="entry name" value="CELF1/2_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034198">
        <property type="entry name" value="CELF1/2_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034199">
        <property type="entry name" value="CELF1/2_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="28"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="120"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="415"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="292"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="500" mass="53495" checksum="131539F809751A2F" modified="2019-05-08" version="1">MERIEGENNSKKMNGTLDHPDQPDLDAIKMFVGQVPRSWCEKDLRELFEQYGAVYEINVLRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNMKILPGMHHPIQMKPADSEKNNAVEDRKLFIGMISKKCNENDIRVMFSSFGQIEECRILRGPDGLSRGCAFVTFTTRAMAQTAIKAMHQAQTMEGCSSPIVVKFADTQKDKEQKRIAQQLQQQMQQISAASVWGNLAGLNTLGPQYLALYLQLLQQTAAASSGNLNTLSSLHPMGGLNAMQLQNLAALAAAASAAQNTPSGTAALTSSSSPLSVLTSSGSSPSSSSSSSVNPMASLGALQTLAGATAGLNVSSLAGMAALNGGLGSGGLSNGTGSTMEALTQAYSGIQQYAAAALPTLYSQSLLTQQSIGAAGSQKEGPEGANLFIYHLPQEFGDQDLLQMFMPFGNVVSAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQSMNGFQIGMKRLKVQLKRSKNDSKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLA4</accession>
    <name>A0A452HLA4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alpha-2-macroglobulin like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HLA4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015718"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017947.1">
        <property type="protein sequence ID" value="ENSGAGP00000015718.1"/>
        <property type="gene ID" value="ENSGAGG00000011844.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF182">
        <property type="entry name" value="ALPHA-2-MACROGLOBULIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="9">
        <location>
            <begin position="216"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="10">
        <location>
            <begin position="494"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="11">
        <location>
            <begin position="1119"/>
            <end position="1199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1241" mass="137517" checksum="02F67C0877F74800" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLJ7</accession>
    <name>A0A452HLJ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPP phosphatase family. PP-2B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452HLJ7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015803"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018048.1">
        <property type="protein sequence ID" value="ENSGAGP00000015803.1"/>
        <property type="gene ID" value="ENSGAGG00000011890.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033192">
        <property type="term" value="F:calmodulin-dependent protein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097720">
        <property type="term" value="P:calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07416">
        <property type="entry name" value="MPP_PP2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041751">
        <property type="entry name" value="MPP_PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043360">
        <property type="entry name" value="PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45673">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="14">
        <location>
            <begin position="150"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="489"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="502"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="518" mass="58388" checksum="5589434328F9D6E1" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLM7</accession>
    <name>A0A452HLM7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgin subfamily A member 7/ERF4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ERF4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HLM7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015836"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018093.1">
        <property type="protein sequence ID" value="ENSGAGP00000015836.1"/>
        <property type="gene ID" value="ENSGAGG00000011915.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002178">
        <property type="term" value="C:palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051371">
        <property type="entry name" value="Ras_palmitoyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254">
        <property type="entry name" value="GOLGI AUTOANTIGEN, GOLGIN SUBFAMILY A, 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254:SF2">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="4">
        <location>
            <begin position="20"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="176" mass="19479" checksum="AA615389576C151C" modified="2019-05-08" version="1">SPQAHDLQELRRSASLATKVFVQRDYSDGTLCQFQTKFPLELDSRLERQLFEETVKTLNGFYAEAEKIGGSSYLEGCLACATAYFIFLCMETHYEKVLKKISKYIQEQNEQVYAPRGLLLTDPVERGMRVVSSWAGQEGGPRGGRGCGAGGFWGDPSVDLLLEWGEGASGQAPWGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HM36</accession>
    <name>A0A452HM36_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fatty acid synthase</fullName>
            <ecNumber evidence="5">1.1.1.100</ecNumber>
            <ecNumber evidence="2">1.3.1.39</ecNumber>
            <ecNumber evidence="8">2.3.1.38</ecNumber>
            <ecNumber evidence="9">2.3.1.39</ecNumber>
            <ecNumber evidence="7">2.3.1.41</ecNumber>
            <ecNumber evidence="4">2.3.1.85</ecNumber>
            <ecNumber evidence="3">3.1.2.14</ecNumber>
            <ecNumber evidence="6">4.2.1.59</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="73 74">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="74" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="73" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="36">Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>(2E)-butenoyl-[ACP] + NADPH + H(+) = butanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41812"/>
            <dbReference type="Rhea" id="RHEA-COMP:9627"/>
            <dbReference type="Rhea" id="RHEA-COMP:9628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78453"/>
            <dbReference type="ChEBI" id="CHEBI:78454"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:41813"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="67">
            <text>(2E)-decenoyl-[ACP] + NADPH + H(+) = decanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41864"/>
            <dbReference type="Rhea" id="RHEA-COMP:9639"/>
            <dbReference type="Rhea" id="RHEA-COMP:9640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78467"/>
            <dbReference type="ChEBI" id="CHEBI:78468"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="67">
            <dbReference type="Rhea" id="RHEA:41865"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="50">
            <text>(2E)-dodecenoyl-[ACP] + NADPH + H(+) = dodecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41880"/>
            <dbReference type="Rhea" id="RHEA-COMP:9643"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="ChEBI" id="CHEBI:78472"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="50">
            <dbReference type="Rhea" id="RHEA:41881"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="45">
            <text>(2E)-hexadecenoyl-[ACP] + NADPH + H(+) = hexadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41912"/>
            <dbReference type="Rhea" id="RHEA-COMP:9651"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78481"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="45">
            <dbReference type="Rhea" id="RHEA:41913"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="46">
            <text>(2E)-hexenoyl-[ACP] + NADPH + H(+) = hexanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41832"/>
            <dbReference type="Rhea" id="RHEA-COMP:9631"/>
            <dbReference type="Rhea" id="RHEA-COMP:9632"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78458"/>
            <dbReference type="ChEBI" id="CHEBI:78459"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="46">
            <dbReference type="Rhea" id="RHEA:41833"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="60">
            <text>(2E)-octadecenoyl-[ACP] + NADPH + H(+) = octadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41928"/>
            <dbReference type="Rhea" id="RHEA-COMP:9655"/>
            <dbReference type="Rhea" id="RHEA-COMP:9656"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78489"/>
            <dbReference type="ChEBI" id="CHEBI:78495"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="60">
            <dbReference type="Rhea" id="RHEA:41929"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="53">
            <text>(2E)-octenoyl-[ACP] + NADPH + H(+) = octanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41848"/>
            <dbReference type="Rhea" id="RHEA-COMP:9635"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78462"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="53">
            <dbReference type="Rhea" id="RHEA:41849"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="62">
            <text>(2E)-tetradecenoyl-[ACP] + NADPH + H(+) = tetradecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41896"/>
            <dbReference type="Rhea" id="RHEA-COMP:9647"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78475"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="62">
            <dbReference type="Rhea" id="RHEA:41897"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>(3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41808"/>
            <dbReference type="Rhea" id="RHEA-COMP:9626"/>
            <dbReference type="Rhea" id="RHEA-COMP:9627"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78451"/>
            <dbReference type="ChEBI" id="CHEBI:78453"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:41809"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>(3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41860"/>
            <dbReference type="Rhea" id="RHEA-COMP:9638"/>
            <dbReference type="Rhea" id="RHEA-COMP:9639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78466"/>
            <dbReference type="ChEBI" id="CHEBI:78467"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:41861"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>(3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41876"/>
            <dbReference type="Rhea" id="RHEA-COMP:9642"/>
            <dbReference type="Rhea" id="RHEA-COMP:9643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78470"/>
            <dbReference type="ChEBI" id="CHEBI:78472"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:41877"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>(3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41908"/>
            <dbReference type="Rhea" id="RHEA-COMP:9650"/>
            <dbReference type="Rhea" id="RHEA-COMP:9651"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78480"/>
            <dbReference type="ChEBI" id="CHEBI:78481"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:41909"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>(3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41828"/>
            <dbReference type="Rhea" id="RHEA-COMP:9630"/>
            <dbReference type="Rhea" id="RHEA-COMP:9631"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78457"/>
            <dbReference type="ChEBI" id="CHEBI:78458"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:41829"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>(3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41924"/>
            <dbReference type="Rhea" id="RHEA-COMP:9654"/>
            <dbReference type="Rhea" id="RHEA-COMP:9655"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78488"/>
            <dbReference type="ChEBI" id="CHEBI:78489"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:41925"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>(3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41844"/>
            <dbReference type="Rhea" id="RHEA-COMP:9634"/>
            <dbReference type="Rhea" id="RHEA-COMP:9635"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78461"/>
            <dbReference type="ChEBI" id="CHEBI:78462"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:41845"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>(3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41892"/>
            <dbReference type="Rhea" id="RHEA-COMP:9646"/>
            <dbReference type="Rhea" id="RHEA-COMP:9647"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78474"/>
            <dbReference type="ChEBI" id="CHEBI:78475"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:41893"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="47">
            <text>3-oxobutanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxybutanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41804"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="Rhea" id="RHEA-COMP:9626"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
            <dbReference type="ChEBI" id="CHEBI:78451"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="47">
            <dbReference type="Rhea" id="RHEA:41805"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>3-oxodecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxydecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41856"/>
            <dbReference type="Rhea" id="RHEA-COMP:9637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9638"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78464"/>
            <dbReference type="ChEBI" id="CHEBI:78466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:41857"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="63">
            <text>3-oxododecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxydodecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41872"/>
            <dbReference type="Rhea" id="RHEA-COMP:9641"/>
            <dbReference type="Rhea" id="RHEA-COMP:9642"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78469"/>
            <dbReference type="ChEBI" id="CHEBI:78470"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="63">
            <dbReference type="Rhea" id="RHEA:41873"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="64">
            <text>3-oxohexadecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyhexadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9650"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78478"/>
            <dbReference type="ChEBI" id="CHEBI:78480"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="64">
            <dbReference type="Rhea" id="RHEA:41905"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="55">
            <text>3-oxohexanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyhexanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41824"/>
            <dbReference type="Rhea" id="RHEA-COMP:9629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9630"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78456"/>
            <dbReference type="ChEBI" id="CHEBI:78457"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="55">
            <dbReference type="Rhea" id="RHEA:41825"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>3-oxooctadecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyoctadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41920"/>
            <dbReference type="Rhea" id="RHEA-COMP:9653"/>
            <dbReference type="Rhea" id="RHEA-COMP:9654"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78487"/>
            <dbReference type="ChEBI" id="CHEBI:78488"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:41921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="65">
            <text>3-oxooctanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyoctanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41840"/>
            <dbReference type="Rhea" id="RHEA-COMP:9633"/>
            <dbReference type="Rhea" id="RHEA-COMP:9634"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78460"/>
            <dbReference type="ChEBI" id="CHEBI:78461"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="65">
            <dbReference type="Rhea" id="RHEA:41841"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="59">
            <text>3-oxotetradecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxytetradecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41888"/>
            <dbReference type="Rhea" id="RHEA-COMP:9645"/>
            <dbReference type="Rhea" id="RHEA-COMP:9646"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78473"/>
            <dbReference type="ChEBI" id="CHEBI:78474"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="59">
            <dbReference type="Rhea" id="RHEA:41889"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="40">
            <dbReference type="Rhea" id="RHEA:17399"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:13098"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="56">
            <text>a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22564"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.39"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="56">
            <dbReference type="Rhea" id="RHEA:22566"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="54">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="54">
            <dbReference type="Rhea" id="RHEA:22837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H(+) = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.85"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="48">
            <text>acetyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxobutanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41800"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="48">
            <dbReference type="Rhea" id="RHEA:41801"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="66">
            <text>butanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41820"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9628"/>
            <dbReference type="Rhea" id="RHEA-COMP:9629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78454"/>
            <dbReference type="ChEBI" id="CHEBI:78456"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="66">
            <dbReference type="Rhea" id="RHEA:41821"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="61">
            <text>decanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41868"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9641"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78468"/>
            <dbReference type="ChEBI" id="CHEBI:78469"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="61">
            <dbReference type="Rhea" id="RHEA:41869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>dodecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41884"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="Rhea" id="RHEA-COMP:9645"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78473"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="44">
            <dbReference type="Rhea" id="RHEA:41885"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="58">
            <text>hexadecanoyl-[ACP] + H2O = hexadecanoate + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41932"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
            <dbReference type="EC" id="3.1.2.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="58">
            <dbReference type="Rhea" id="RHEA:41933"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="49">
            <text>hexadecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctadecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9653"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
            <dbReference type="ChEBI" id="CHEBI:78487"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="49">
            <dbReference type="Rhea" id="RHEA:41917"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>hexanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9632"/>
            <dbReference type="Rhea" id="RHEA-COMP:9633"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78459"/>
            <dbReference type="ChEBI" id="CHEBI:78460"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:41837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="57">
            <text>holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41788"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="EC" id="2.3.1.38"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="57">
            <dbReference type="Rhea" id="RHEA:41789"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="52">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="52">
            <dbReference type="Rhea" id="RHEA:41793"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="68">
            <text>octanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41852"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78464"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="68">
            <dbReference type="Rhea" id="RHEA:41853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="51">
            <text>tetradecanoyl-[ACP] + H2O = tetradecanoate + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30123"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
            <dbReference type="EC" id="3.1.2.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="51">
            <dbReference type="Rhea" id="RHEA:30124"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>tetradecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexadecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41900"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="Rhea" id="RHEA-COMP:9649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
            <dbReference type="ChEBI" id="CHEBI:78478"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:41901"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="5"/>
    <dbReference type="EC" id="1.3.1.39" evidence="2"/>
    <dbReference type="EC" id="2.3.1.38" evidence="8"/>
    <dbReference type="EC" id="2.3.1.39" evidence="9"/>
    <dbReference type="EC" id="2.3.1.41" evidence="7"/>
    <dbReference type="EC" id="2.3.1.85" evidence="4"/>
    <dbReference type="EC" id="3.1.2.14" evidence="3"/>
    <dbReference type="EC" id="4.2.1.59" evidence="6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016001"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018281.1">
        <property type="protein sequence ID" value="ENSGAGP00000016001.1"/>
        <property type="gene ID" value="ENSGAGG00000012026.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000018294.1">
        <property type="protein sequence ID" value="ENSGAGP00000016014.1"/>
        <property type="gene ID" value="ENSGAGG00000012026.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000018302.1">
        <property type="protein sequence ID" value="ENSGAGP00000016020.1"/>
        <property type="gene ID" value="ENSGAGG00000012026.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004313">
        <property type="term" value="F:[acyl-carrier-protein] S-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141148">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08954">
        <property type="entry name" value="KR_1_FAS_SDR_x"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000013">
        <property type="entry name" value="Fatty acid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.110:FF:000002">
        <property type="entry name" value="Fatty acid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000005">
        <property type="entry name" value="Fatty acid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000033">
        <property type="entry name" value="Fatty acid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000059">
        <property type="entry name" value="Fatty acid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000105">
        <property type="entry name" value="Fatty acid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000249">
        <property type="entry name" value="Fatty acid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000015">
        <property type="entry name" value="Fatty acid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000209">
        <property type="entry name" value="Polyketide synthase Pks12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049391">
        <property type="entry name" value="FAS_pseudo-KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020807">
        <property type="entry name" value="PKS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001031">
        <property type="entry name" value="Thioesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF7">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21149">
        <property type="entry name" value="FAS_pseudo-KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00975">
        <property type="entry name" value="Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00826">
        <property type="entry name" value="PKS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="20" id="KW-0007">Acetylation</keyword>
    <keyword evidence="24" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="17" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="23" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="25" id="KW-0456">Lyase</keyword>
    <keyword evidence="26" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="22" id="KW-0520">NAD</keyword>
    <keyword evidence="18" id="KW-0521">NADP</keyword>
    <keyword evidence="21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="74" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0702">S-nitrosylation</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="71">
        <location>
            <begin position="1"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="72">
        <location>
            <begin position="844"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="70">
        <location>
            <begin position="2124"/>
            <end position="2201"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="69">
        <location>
            <begin position="844"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="69">
        <location>
            <begin position="984"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="69">
        <location>
            <position position="878"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="69">
        <location>
            <position position="1034"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00013258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00018769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00023373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00023388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00023394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00023398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00023399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00023401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00023402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00023442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00044883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00047300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00047394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00047400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00047440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00047451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="ARBA" id="ARBA00047500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
            <dbReference type="ARBA" id="ARBA00047578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="45">
        <source>
            <dbReference type="ARBA" id="ARBA00047810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="46">
        <source>
            <dbReference type="ARBA" id="ARBA00047897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="47">
        <source>
            <dbReference type="ARBA" id="ARBA00047953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="48">
        <source>
            <dbReference type="ARBA" id="ARBA00047961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="49">
        <source>
            <dbReference type="ARBA" id="ARBA00048051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="50">
        <source>
            <dbReference type="ARBA" id="ARBA00048281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="51">
        <source>
            <dbReference type="ARBA" id="ARBA00048289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="52">
        <source>
            <dbReference type="ARBA" id="ARBA00048404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="53">
        <source>
            <dbReference type="ARBA" id="ARBA00048420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="54">
        <source>
            <dbReference type="ARBA" id="ARBA00048506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="55">
        <source>
            <dbReference type="ARBA" id="ARBA00048571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="56">
        <source>
            <dbReference type="ARBA" id="ARBA00048650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="57">
        <source>
            <dbReference type="ARBA" id="ARBA00048691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="58">
        <source>
            <dbReference type="ARBA" id="ARBA00048704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="59">
        <source>
            <dbReference type="ARBA" id="ARBA00048935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="60">
        <source>
            <dbReference type="ARBA" id="ARBA00049019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="61">
        <source>
            <dbReference type="ARBA" id="ARBA00049109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="62">
        <source>
            <dbReference type="ARBA" id="ARBA00049171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="63">
        <source>
            <dbReference type="ARBA" id="ARBA00049263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="64">
        <source>
            <dbReference type="ARBA" id="ARBA00049414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="65">
        <source>
            <dbReference type="ARBA" id="ARBA00049422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="66">
        <source>
            <dbReference type="ARBA" id="ARBA00049449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="67">
        <source>
            <dbReference type="ARBA" id="ARBA00049521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="68">
        <source>
            <dbReference type="ARBA" id="ARBA00049533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="69">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="70">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="71">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="72">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="73">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="74">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2513" mass="276181" checksum="344A013434A43F4A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HP24</accession>
    <name>A0A452HP24_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SOCS box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HP24"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016721"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019089.1">
        <property type="protein sequence ID" value="ENSGAGP00000016721.1"/>
        <property type="gene ID" value="ENSGAGG00000012503.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF187">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX CONTAINING 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="113"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="179"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="212"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="245"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="310"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="352"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="382"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="5">
        <location>
            <begin position="536"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="591" mass="66212" checksum="A52B81B903EFBD0C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HPY3</accession>
    <name>A0A452HPY3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cell adhesion molecule-related/down-regulated by oncogenes</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000017063"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019471.1">
        <property type="protein sequence ID" value="ENSGAGP00000017063.1"/>
        <property type="gene ID" value="ENSGAGG00000012690.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000205">
        <property type="entry name" value="Cell adhesion associated, oncogene regulated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000327">
        <property type="entry name" value="Cell adhesion associated, oncogene regulated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000352">
        <property type="entry name" value="Cell adhesion molecule-related/down-regulated by oncogenes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000273">
        <property type="entry name" value="contactin-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF1">
        <property type="entry name" value="CELL ADHESION MOLECULE-RELATED_DOWN-REGULATED BY ONCOGENES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019530137" description="Cell adhesion molecule-related/down-regulated by oncogenes" evidence="14">
        <location>
            <begin position="26"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="956"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="29"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="131"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="225"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="310"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="405"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="574"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="712"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="816"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="660"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="921"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1243"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="666"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1267" mass="138011" checksum="40C8B57411C07E5E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQ46</accession>
    <name>A0A452HQ46_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine-threonine kinase receptor-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQ46"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017141"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019563.1">
        <property type="protein sequence ID" value="ENSGAGP00000017141.1"/>
        <property type="gene ID" value="ENSGAGG00000012803.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030512">
        <property type="term" value="P:negative regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000133">
        <property type="entry name" value="Serine-threonine kinase receptor-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF13">
        <property type="entry name" value="SERINE-THREONINE KINASE RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="55"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="96"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="139"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="319"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="335"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="350" mass="38312" checksum="A40865E3E7E0A3E3" modified="2019-05-08" version="1">MAMRQTPLTCSGHTRPVVDLAFSDITPYGYFLISACKDGKPMLRQGDTGDWIGTFLGHKGAVWGATLNRDATKAATAAADFTAKVWDAVSGDELITLAHKHIVKSVDFTEDSNYLLTGGQDKLLRIYDLSKPEAEPQVVSGHTSGIKKALWSSDDKQILSADDKTVRLWDRNTMTEVKAINVSMSVSSMEYVPEGEILVVTYGRTIAFHSAETLEQIKSFEAPATVNSASLHPEKEFLVAGGEDFKLYKYDYNTGEELESYKGHFGPIHCVRFSPDGELYASGSEDGTLRLWQTTVGKTYGLWKCVVPEEEGGELAKARVSLPGTAEEELEDLASENSDSIYSSTPEVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRC5</accession>
    <name>A0A452HRC5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HRC5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017551"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020013.1">
        <property type="protein sequence ID" value="ENSGAGP00000017549.1"/>
        <property type="gene ID" value="ENSGAGG00000013034.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000020016.1">
        <property type="protein sequence ID" value="ENSGAGP00000017551.1"/>
        <property type="gene ID" value="ENSGAGG00000013034.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072383">
        <property type="term" value="P:plus-end-directed vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901098">
        <property type="term" value="P:positive regulation of autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15745">
        <property type="entry name" value="FYVE_RUFY4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059036">
        <property type="entry name" value="RUFY4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46753">
        <property type="entry name" value="FYVE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46753:SF5">
        <property type="entry name" value="RUN AND FYVE DOMAIN CONTAINING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25366">
        <property type="entry name" value="RUFY4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="912"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="295"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="369"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="583"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="812"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="313"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="968" mass="107676" checksum="CD17D8B0A0D7E03F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HST4</accession>
    <name>A0A452HST4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HST4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018154"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020692.1">
        <property type="protein sequence ID" value="ENSGAGP00000018154.1"/>
        <property type="gene ID" value="ENSGAGG00000013447.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001872">
        <property type="term" value="F:(1-&gt;3)-beta-D-glucan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071226">
        <property type="term" value="P:cellular response to molecule of fungal origin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042808">
        <property type="entry name" value="CLEC7A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47218">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 7 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47218:SF2">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="77"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="216" mass="24490" checksum="3B34988BE7D50198" modified="2019-05-08" version="1">MILSEHWNSLSSFEGQKHSDAVHCTEIYIPNFLKYNWLSSNFHSTSPFSFTCNVQFLNACIFKGNKSTHYPEKWMQHRQDCYHFSNSWKPCKALCSSLDSRFLKIESKEELGFIIRSAQSYSSYSLWIALSRKGADGSWLWEDGRAFSTDLISAAPWVPGGGHWCQWVTNFRPDCKVPKSICEAITPCSPAAHWGNHFLQITCQGLAVCSGARGMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTB6</accession>
    <name>A0A452HTB6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytohesin Ubiquitin Protein Inducing domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HTB6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018337"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020900.1">
        <property type="protein sequence ID" value="ENSGAGP00000018337.1"/>
        <property type="gene ID" value="ENSGAGG00000013581.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034334">
        <property type="term" value="P:adherens junction maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043447">
        <property type="entry name" value="CCDC120/INAVA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021774">
        <property type="entry name" value="CUPID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16093">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 120 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16093:SF4">
        <property type="entry name" value="INNATE IMMUNITY ACTIVATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11819">
        <property type="entry name" value="CUPID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytohesin Ubiquitin Protein Inducing" evidence="6">
        <location>
            <begin position="2"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="231"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="318"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="383"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="258"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="383"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="611" mass="67308" checksum="B9FDC33B63CC4503" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTN5</accession>
    <name>A0A452HTN5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HTN5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018459"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021040.1">
        <property type="protein sequence ID" value="ENSGAGP00000018459.1"/>
        <property type="gene ID" value="ENSGAGG00000013666.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15229">
        <property type="entry name" value="7tmA_OR8S1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="310" mass="34961" checksum="31AEDE66060D2DA9" modified="2019-05-08" version="1">MKNQTTVTEFILVGLSSEPQMQIFLFLVFLVIYLITLVGNIVIMVVIRAHFHLHTPMYFFLFHLSFVDICYSSVTVPNGLRNFLAEHKTISVNGCIAQMFFILLSGAAESFILSAMAYDRYAAICDPLRYMEIMRKGICVQLVSGAWTIGLIYALLNTIFTLKLHFCGSNQIHHFSCELPPLLQLACTETLTNQVVLLTSVVIFGSISFLLTLISYIYIIITILRIRSAEGRHKAFSTCSSHLIVVGLLYLTGFLQYTKPSSVSSVLLDEIFSIQYSVLTPMLNPIIYSLKNKEVKTALRKTLGKLKFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HU31</accession>
    <name>A0A452HU31_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Regulation of nuclear pre-mRNA domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Associates with the RNA polymerase II complex.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000018610"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021217.1">
        <property type="protein sequence ID" value="ENSGAGP00000018610.1"/>
        <property type="gene ID" value="ENSGAGG00000013770.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099122">
        <property type="term" value="F:RNA polymerase II C-terminal domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17001">
        <property type="entry name" value="CID_RPRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000020">
        <property type="entry name" value="regulation of nuclear pre-mRNA domain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047885">
        <property type="entry name" value="RPRD2_CID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460:SF40">
        <property type="entry name" value="REGULATION OF NUCLEAR PRE-MRNA DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="7">
        <location>
            <begin position="18"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="311"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="470"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="555"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="701"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="905"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1020"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1192"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1373"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="352"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="379"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="474"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="555"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="580"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="635"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="701"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="744"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="760"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="912"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="945"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="979"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1039"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1223"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1238"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1404"/>
            <end position="1421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1448" mass="156175" checksum="2F2B06EC5AE0C0C4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUS4</accession>
    <name>A0A452HUS4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000018827"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021462.1">
        <property type="protein sequence ID" value="ENSGAGP00000018827.1"/>
        <property type="gene ID" value="ENSGAGG00000013933.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000005">
        <property type="entry name" value="Zinc finger protein 14 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000362">
        <property type="entry name" value="Zinc finger protein 606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000011">
        <property type="entry name" value="zinc finger protein 615 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="18"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="435" mass="48758" checksum="5F8BF77599EA2457" modified="2019-05-08" version="1">MIENLEKVLGMDRKVTLHKHPLPSPHVSVSPRAAAPGTPRLTPVSSGMELQGTFLRRVDGNFSQCLEQRKGWSNWRGSERKLGNCRRKKVDESIDCGGGGKDPKETTAQQTSHKEKKPYKCLDCGKSFNYPSVLINHKIIHTRDRLYKYVECGKSYNQRSALVTHGRIHTGERPYKCLDNGKNFNVSSHLITHRRVHTGERPYKCLESGKSFSQCSHLITHQRLHTGERPYKCLGCGESFSQQSVLINHGRIHTGERPYKCLDGGKSFSQSSHLITHQRVHTGERPYKCLECWKTNQNSLHIRESTWERNHINAWMMGKVSVPKLPLPGVPPLQSLPRCDAPSMERDTWARPHIYSGHLSSSSSCPWTRLPHLPGAGPCRASVGLQGIKCGGTRRNGLWGANWXLTPHGTSETPPQRTEVLCALTSAGHSKNSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HV28</accession>
    <name>A0A452HV28_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HV28"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018999"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021658.1">
        <property type="protein sequence ID" value="ENSGAGP00000018999.1"/>
        <property type="gene ID" value="ENSGAGG00000014057.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF30">
        <property type="entry name" value="THAP-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="241" mass="26675" checksum="A0EE07AF69E41521" modified="2019-05-08" version="1">MPYGKCCVGFSVHINLISIIRLLHDGMTATILCNNSQTEPFVIHTGVKQGCVIAPTLFSIYLVVILILIRDHLPGGIGIEIDITDLRYAVDCIILAHTEADLQSTLNFFADAYHSLGLSLNTGKTKVLYQPSPAQTTFHTSQMTISGNPLENVDHFPYLGSHFSQTASIDTEIEYRVCCASTSFGRLLKRVFNDRALQTGTKILVYKTVVIPTLLYGCEIWHDNIPDQSNAQLSFLAIQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWF3</accession>
    <name>A0A452HWF3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UBX domain-containing protein 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">UBX domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May negatively regulate the ATPase activity of VCP, an ATP-driven segregase that associates with different cofactors to control a wide variety of cellular processes. As a cofactor of VCP, it may play a role in the transport of CAV1 to lysosomes for degradation. It may also play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Together with VCP and other cofactors, it may play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with VCP through the PUB domain (via C-terminus) and VIM motif (via N-terminus); the interaction is direct. Forms a ternary complex with CAV1 and VCP. Interacts with SYVN1. Interacts with HERPUD1. Interacts with VCPKMT. May interact with DERL1. Interacts with PLAA, VCP and YOD1; may form a complex involved in macroautophagy. Interacts with LMAN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HWF3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019488"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022191.1">
        <property type="protein sequence ID" value="ENSGAGP00000019488.1"/>
        <property type="gene ID" value="ENSGAGG00000014369.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10460">
        <property type="entry name" value="PUB_UBXD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16119">
        <property type="entry name" value="UBX_UBXN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000185">
        <property type="entry name" value="UBX domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036339">
        <property type="entry name" value="PUB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018997">
        <property type="entry name" value="PUB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042774">
        <property type="entry name" value="UBXN6_PUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23153:SF38">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23153">
        <property type="entry name" value="UBX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09409">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143503">
        <property type="entry name" value="PUG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBX" evidence="8">
        <location>
            <begin position="245"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00059509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="354" mass="40486" checksum="54E059B843291738" modified="2019-05-08" version="1">MAEAGASERGLSTDHKGHVSAVEEETAGLSVSGVYFICPLTGATVRKDQKEAHIKEAILLHSSRDPVAASIMEIHTFNRDREKVKLGVETIAKYLDNIHLHPEEEKYRKIKLQNKVFQERINCLEGTHEFFQAIGFEKEMLPVPGQETTEEYYVLKKEALTKLEDLKDYKEQLLSSEPVKARLDRQLCVFKPSPQAAQFELPHDFYNLTAEELKREQRLRTEAVEKASMLRTKAMREREEQREMRKYNYTLLRVRFPDGYILQGTFYAREAVSELYRFVREALKDDWMPFELLAPGGLKLTEENLAFNECGLVPSALLTFAWDAAVMADIKASGEEQKGSPLKPELLSAVQILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I141</accession>
    <name>A0A452I141_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452I141"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021177"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024118.1">
        <property type="protein sequence ID" value="ENSGAGP00000021177.1"/>
        <property type="gene ID" value="ENSGAGG00000015562.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14165">
        <property type="entry name" value="STKc_TSSK1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000443">
        <property type="entry name" value="Testis-specific serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF102">
        <property type="entry name" value="TESTIS-SPECIFIC SERINE_THREONINE-PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="17" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="12"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="361" mass="41558" checksum="00D25C41A1BF29AD" modified="2019-05-08" version="1">MDDAAILKKRGYVLGINLGEGSYAKVKSAYSDRLKFNVAVKIINKKKAPRDFLERFLPREIEILAKVNHRAIVKTYEIFETSDGKVYIVMELGVQGDLLEFIKSRGAISEDVARKMFHQLSSAIKYCHDLDVVHRDLKCENLLLDKDFNIKLSDFGFSRRLTRDENGKPILSKTFCGSAAYAAPEVLQGIPYEPKIYDIWSLGVILFIMVCGSMPYDDSNIGKMLKIQKEHRVHFPKSKSLTVECKDLIYRMLQPDVARRLHIEEVFNHMWMQAPKPRVPLATSPVKEGECSRNLREFKSDMKLRGKYWQLYHSVNVKVTYHLGEESRVWVDHHLILVKDGEWKLSHEGTEFTQGPGNLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I337</accession>
    <name>A0A452I337_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I337"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021929"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024988.1">
        <property type="protein sequence ID" value="ENSGAGP00000021929.1"/>
        <property type="gene ID" value="ENSGAGG00000016102.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18388">
        <property type="entry name" value="BTB_POZ_KCTD15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000003">
        <property type="entry name" value="BTB/POZ domain-containing protein KCTD6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048595">
        <property type="entry name" value="KCTD1-15-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045904">
        <property type="entry name" value="KCTD15_T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF27">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20871">
        <property type="entry name" value="KCTD1-15_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="35"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="261" mass="29784" checksum="D9FF55CF0F32CED6" modified="2019-05-08" version="1">MEGRSMSRLSLTRSPVSPLAAQGIPLPAQLTKSNAPVHIDVGGHMYTSSLATLTKYPDSRISRLFNGTEPIVLDSLKQHYFIDRDGEIFRYILSFLRTSKLLLPDDFKDFNLLYEEAKYYQLQPMIKELERWKQEKEQRKHFQPCDCLVVRVTPDLGERIALSGEKALIEEIFPETGDVMCNSVNAGWNQDPTHVIRFPLNGYCRLNSVQVLERLFQKGFNVAASCGGGVDSSQFSEYVLCREDRRPQPTPTIRIKQEPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3D7</accession>
    <name>A0A452I3D7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Thioredoxin domain-containing protein 5</fullName>
            <ecNumber evidence="4">5.3.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Endoplasmic reticulum resident protein 46</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Thioredoxin-like protein p46</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Protein disulfide isomerase of the endoplasmic reticulum lumen involved in the formation of disulfide bonds in proteins. Can reduce insulin disulfide bonds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452I3D7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022003"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025068.1">
        <property type="protein sequence ID" value="ENSGAGP00000022003.1"/>
        <property type="gene ID" value="ENSGAGG00000016140.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03005">
        <property type="entry name" value="PDI_a_ERp46"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000146">
        <property type="entry name" value="Thioredoxin domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000164">
        <property type="entry name" value="Thioredoxin domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000147">
        <property type="entry name" value="Thioredoxin domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019458225" description="Thioredoxin domain-containing protein 5" evidence="18">
        <location>
            <begin position="36"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="19">
        <location>
            <begin position="30"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="19">
        <location>
            <begin position="157"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="19">
        <location>
            <begin position="286"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="277"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="414" mass="46753" checksum="8B30BA0921E69B8C" modified="2019-05-08" version="1">MAAALCRGASPRPGPARLLLLLGGLLCAAPARGDAEPEPELDPHARHRYNAEMLRHGAQIAPHFVMFFAPWCGHCQRLQPTWNDLGDKYNNMENPSVYVVKVDCTIDIPLCSEFGIRGYPTLKLLKPGQEPVKYQGPRDFQALESWMLQTLNEEPAEQESDLQPPKLPEPKQGMHDLSAANFKMHIEEGNHFIKFFAPWCGHCKALAPTWEQLASVLEHSETVKIGRVDCTQHYEVCSENQVRGYPTLLWFSNGEKADQYKGKRDLDSLKEYVDSQLQNAEKAPDADKPPEVPQQPAEPTQDERTVLSLSEKDFDQTIAKGVTFIKFYAPWCGHCKNLAPTWENLSKEQFPGLTDVKIAKVDCTIERNVCNRFSVNGYPTLLLFRAGEKVSEHSGARDLETLHKFVMRQARDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4P1</accession>
    <name>A0A452I4P1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dynein axonemal assembly factor 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the assembly of axonemal inner and outer dynein arms. Involved in preassembly of dyneins into complexes before their transport into cilia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Dynein axonemal particle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNAAF3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I4P1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022485"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025615.1">
        <property type="protein sequence ID" value="ENSGAGP00000022485.1"/>
        <property type="gene ID" value="ENSGAGG00000016492.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039304">
        <property type="entry name" value="DNAAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028235">
        <property type="entry name" value="DNAAF3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027974">
        <property type="entry name" value="DUF4470"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22118">
        <property type="entry name" value="DYNEIN ASSEMBLY FACTOR 3, AXONEMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22118:SF14">
        <property type="entry name" value="DYNEIN AXONEMAL ASSEMBLY FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14737">
        <property type="entry name" value="DUF4470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14740">
        <property type="entry name" value="DUF4471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4470" evidence="8">
        <location>
            <begin position="14"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein assembly factor 3 C-terminal" evidence="9">
        <location>
            <begin position="152"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="323"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="427" mass="47942" checksum="845DE9E4CDB76151" modified="2019-05-08" version="1">MTTGAGSSFGTTCWWGFSPALDLQRECLESSMAHLCLSQDDLPELNILLVGSVDGRHVLRTMSQAHRWPQRRINFYIVENNLEVLGRQLLFLSLALESPEKMGLQEKSEMFLELLGNSLIRSPTAAYLQEKSDLFIRYVTDPDFQQRHLGAVDLSALKFKERDQLESIFRFWRNPDPQAFPIARLWDLRVRQYLGTRYDARRGVCDWDLTMKLHERGARTIGGREFSRWRDTGVAFELREGVYHIPNKTLASGRLLRHKGELVPSRGYWGDIGTGPFLPFGIQSEEPSLLKTINGIPAKSAQEISLYNVTSLFHELAARAPFPPPPPIPEGASGAALAQESPAPNPDPDLQDHKAACPEHVRIHFLPLGCTARLPCKSQYHQHFHLLFFSCSMVHALTPELQLVSAPRATLITGVAAGICPQGYADH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5R5</accession>
    <name>A0A452I5R5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dapper family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I5R5"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026136.1">
        <property type="protein sequence ID" value="ENSGAGP00000022935.1"/>
        <property type="gene ID" value="ENSGAGG00000016825.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000026138.1">
        <property type="protein sequence ID" value="ENSGAGP00000022937.1"/>
        <property type="gene ID" value="ENSGAGG00000016825.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900108">
        <property type="term" value="P:negative regulation of nodal signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024843">
        <property type="entry name" value="Dapper"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15919:SF13">
        <property type="entry name" value="DAPPER HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15919">
        <property type="entry name" value="DAPPER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15268">
        <property type="entry name" value="Dapper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="131"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="304"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="402"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="402"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="686" mass="73957" checksum="5D4BB723BE104E4A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5V1</accession>
    <name>A0A452I5V1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I5V1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022974"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026180.1">
        <property type="protein sequence ID" value="ENSGAGP00000022974.1"/>
        <property type="gene ID" value="ENSGAGG00000016854.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000026182.1">
        <property type="protein sequence ID" value="ENSGAGP00000022976.1"/>
        <property type="gene ID" value="ENSGAGG00000016854.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000026184.1">
        <property type="protein sequence ID" value="ENSGAGP00000022978.1"/>
        <property type="gene ID" value="ENSGAGG00000016854.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000013">
        <property type="entry name" value="cell adhesion molecule 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003585">
        <property type="entry name" value="Neurexin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889:SF5">
        <property type="entry name" value="CELL ADHESION MOLECULE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00294">
        <property type="entry name" value="4.1m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604546" description="Ig-like domain-containing protein" evidence="11">
        <location>
            <begin position="24"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="29"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="129"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="229"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="394" mass="42837" checksum="455D194621DBC971" modified="2019-05-08" version="1">MLPPARTLLLLLGCLAAGSGTLSQDESQPTTSDETVVAGGTVVLKCKVEDPDDSSLQWSNPAQQTLYFGEKRALRDNRIQLVNSTPKELAISISNVVLADEGEYTCSIFTMPVRTAKALVTVLGIPQNPQIFGYELPIRETETAQLTCRSSGSKPAAQLRWKKGNKELQGEAPEVTEDSNGKTFTVTSRVEFTVTKEDNDVEVTCTVDHESLQNSERSTSQKLQVYFKPTATIEPDPPYPKEGDALRLQCEAQGNPIPQEFLWTKEGSDVPLHLNTNRVLTLPFLNKSDSGTYICMATSTMGSVVVKYNLDINDASRMSSPSSTYHAVIGGVGAVIVFLLLSLLIVLAQYLIRHKGTYLTHEAKGSDDAPDADTAIINAEGGQASGDDKKEYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I647</accession>
    <name>A0A452I647_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="12">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C19 family. USP10 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452I647"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023038"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026255.1">
        <property type="protein sequence ID" value="ENSGAGP00000023038.1"/>
        <property type="gene ID" value="ENSGAGG00000016089.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030330">
        <property type="term" value="P:DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009818">
        <property type="entry name" value="PAM2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF687">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07145">
        <property type="entry name" value="PAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="15">
        <location>
            <begin position="432"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="145"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="198"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="323"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="572"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="217"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="323"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="582"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="816" mass="90053" checksum="23C8E7C7975BA0C4" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6N8</accession>
    <name>A0A452I6N8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">28S rRNA (cytosine-C(5))-methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">NOL1/NOP2/Sun domain family member 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I6N8"/>
    <dbReference type="SMR" id="A0A452I6N8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023258"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026497.1">
        <property type="protein sequence ID" value="ENSGAGP00000023258.1"/>
        <property type="gene ID" value="ENSGAGG00000017057.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009383">
        <property type="term" value="F:rRNA (cytosine-C5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1170:FF:000004">
        <property type="entry name" value="probable 28S rRNA (Cytosine-C(5))-methyltransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000139">
        <property type="entry name" value="probable 28S rRNA (Cytosine-C(5))-methyltransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049561">
        <property type="entry name" value="NSUN5_7_fdxn-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048889">
        <property type="entry name" value="NSUN5_RCM1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF4">
        <property type="entry name" value="28S RRNA (CYTOSINE-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21148">
        <property type="entry name" value="NSUN5_fdxn-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21153">
        <property type="entry name" value="NSUN5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="14">
        <location>
            <begin position="72"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="373"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="403"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="180"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="417" mass="45918" checksum="60EE4376C4BC44E4" modified="2019-05-08" version="1">MDGAALLKAEKKLPPYLAKVLVYDLLFGKGLKCGGRWKVLVLKHRARLQAELARLKVHRKVSCNEDLLAPAEGVIQAPQVPRYVRVNTLKTCKDDVVDYFKRQGYSYLGRAARMEELSSLSGKQFLLDPHLPELLVFPPQTDFHENRLYVAGHIILQDKASCLPAFLLSPPSGSHVIDACAAPGNKTSHVAAILKNKGQIFAFDLDPNRLATMSTMLVRAGASCCELANQDFLTVDPSDPKYSHVTYILLDPSCSGSGMVKRLPAEEGAPSSERLRALARFQRKALTHALRFPALQRLAYSTCSVQQEENEDVVWDVLQEHGTAFRLVNLLPSWPQRGLATFPGAECCLRACPAATLTNGFFVAVLERQQECNSQDSSSSLPAEADVDSGAERSQNGEGRGPHAARKKRRKKSQCVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I704</accession>
    <name>A0A452I704_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I704"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023415"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026669.1">
        <property type="protein sequence ID" value="ENSGAGP00000023415.1"/>
        <property type="gene ID" value="ENSGAGG00000017157.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000026670.1">
        <property type="protein sequence ID" value="ENSGAGP00000023416.1"/>
        <property type="gene ID" value="ENSGAGG00000017157.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="10">
        <location>
            <begin position="9"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="75" mass="8500" checksum="B2800321484B5D08" modified="2019-05-08" version="1">MKEIMSNNNTTNISQARKAVEQLKMEAYMDRIKVSKAAADLLAYCDAYIGEDPLIIPVPASENPFREKKFFCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7C8</accession>
    <name>A0A452I7C8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways. AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons. AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Adaptor protein complex 4 (AP-4) is a heterotetramer composed of two large adaptins (epsilon-type subunit AP4E1 and beta-type subunit AP4B1), a medium adaptin (mu-type subunit AP4M1) and a small adaptin (sigma-type AP4S1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I7C8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023534"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026820.1">
        <property type="protein sequence ID" value="ENSGAGP00000023534.1"/>
        <property type="gene ID" value="ENSGAGG00000017254.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000026822.1">
        <property type="protein sequence ID" value="ENSGAGP00000023536.1"/>
        <property type="gene ID" value="ENSGAGG00000017254.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14832">
        <property type="entry name" value="AP4_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000010">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="9">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="144" mass="17167" checksum="1AA82C854306B01D" modified="2019-05-08" version="1">MIKFFLMVNKQGQTRLSRYYEHVEIHKRTMLEAEVIKNCLSRSREQCSFIEYKDFKLVYRQYAALFIVVGINETENEMAVYELIHNFVEVLDRYFSRVSELDIMFNLDRVHIILDEMVLNGCIVETNKNRILAPLFVLDKMAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8L4</accession>
    <name>A0A452I8L4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Peptidase metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="19">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="19">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I8L4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024000"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027333.1">
        <property type="protein sequence ID" value="ENSGAGP00000024000.1"/>
        <property type="gene ID" value="ENSGAGG00000017555.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000005">
        <property type="entry name" value="Matrix metallopeptidase 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000001">
        <property type="entry name" value="Matrix metallopeptidase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021805">
        <property type="entry name" value="Pept_M10A_metallopeptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF26">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11857">
        <property type="entry name" value="DUF3377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="15" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="507"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="24">
        <location>
            <begin position="64"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="21">
        <location>
            <begin position="283"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="21">
        <location>
            <begin position="332"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="21">
        <location>
            <begin position="379"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="21">
        <location>
            <begin position="428"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="224"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="22">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="active site" evidence="17">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="19">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine; by PKDCC" evidence="20">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="550" mass="63027" checksum="12CC52F02D04A42D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8Z9</accession>
    <name>A0A452I8Z9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000024149"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027497.1">
        <property type="protein sequence ID" value="ENSGAGP00000024149.1"/>
        <property type="gene ID" value="ENSGAGG00000017663.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000106">
        <property type="entry name" value="Neuroblast differentiation-associated protein AHNAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052082">
        <property type="entry name" value="Myelin_sheath_structural"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23348:SF41">
        <property type="entry name" value="NEUROBLAST DIFFERENTIATION-ASSOCIATED PROTEIN AHNAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23348">
        <property type="entry name" value="PERIAXIN/AHNAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="10"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="213"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="648"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="843"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="917"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="978"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1032"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1253"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1630"/>
            <end position="1661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1777"/>
            <end position="1802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2036"/>
            <end position="2058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2114"/>
            <end position="2144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2251"/>
            <end position="2274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2311"/>
            <end position="2333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2446"/>
            <end position="2477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2593"/>
            <end position="2620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2782"/>
            <end position="2806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2910"/>
            <end position="2934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3032"/>
            <end position="3063"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3106"/>
            <end position="3136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3179"/>
            <end position="3210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3254"/>
            <end position="3285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3327"/>
            <end position="3358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3584"/>
            <end position="3617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3792"/>
            <end position="3814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3833"/>
            <end position="3866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3969"/>
            <end position="3991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="4048"/>
            <end position="4087"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="4129"/>
            <end position="4160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="4331"/>
            <end position="4362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="4480"/>
            <end position="4508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="4541"/>
            <end position="4571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="4592"/>
            <end position="4640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="4754"/>
            <end position="4785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="5540"/>
            <end position="5708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="863"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="923"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1267"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2045"/>
            <end position="2054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2114"/>
            <end position="2128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2446"/>
            <end position="2458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2468"/>
            <end position="2477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3106"/>
            <end position="3118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3179"/>
            <end position="3192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3254"/>
            <end position="3266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3276"/>
            <end position="3285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3327"/>
            <end position="3340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3606"/>
            <end position="3617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3797"/>
            <end position="3806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3976"/>
            <end position="3991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="4052"/>
            <end position="4065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="4078"/>
            <end position="4087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="4129"/>
            <end position="4142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="4331"/>
            <end position="4344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="4480"/>
            <end position="4492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="4548"/>
            <end position="4571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="4630"/>
            <end position="4640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="4762"/>
            <end position="4772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="5708" mass="611285" checksum="0FF20CC9AE3CD892" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IA89</accession>
    <name>A0A452IA89_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Bifunctional glutamate/proline--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.15</ecNumber>
            <ecNumber evidence="5">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Bifunctional aminoacyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:23541"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:14306"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23">In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="4"/>
    <dbReference type="EC" id="6.1.1.17" evidence="5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024609"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028007.1">
        <property type="protein sequence ID" value="ENSGAGP00000024609.1"/>
        <property type="gene ID" value="ENSGAGG00000017949.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097452">
        <property type="term" value="C:GAIT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071346">
        <property type="term" value="P:cellular response to type II interferon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140212">
        <property type="term" value="P:regulation of long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00807">
        <property type="entry name" value="GlnRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10309">
        <property type="entry name" value="GST_C_GluProRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00862">
        <property type="entry name" value="ProRS_anticodon_zinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00936">
        <property type="entry name" value="WEPRS_RNA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000004">
        <property type="entry name" value="bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000006">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.130:FF:000004">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000005">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.30:FF:000001">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000007">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000070">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000005">
        <property type="entry name" value="bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1160.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.800.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1160.10">
        <property type="entry name" value="Glutamyl-trna Synthetase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.800.10">
        <property type="entry name" value="Glutamyl-tRNA Synthetase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020061">
        <property type="entry name" value="Glu_tRNA_lig_a-bdl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000738">
        <property type="entry name" value="WHEP-TRS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382:SF2">
        <property type="entry name" value="BIFUNCTIONAL GLUTAMATE_PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00458">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00991">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00762">
        <property type="entry name" value="WHEP_TRS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51185">
        <property type="entry name" value="WHEP_TRS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0007">Acetylation</keyword>
    <keyword evidence="19" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="20" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="14" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="WHEP-TRS" evidence="28">
        <location>
            <begin position="759"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="WHEP-TRS" evidence="28">
        <location>
            <begin position="835"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="WHEP-TRS" evidence="28">
        <location>
            <begin position="905"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="domain" description="WHEP-TRS" evidence="28">
        <location>
            <begin position="990"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="domain" description="WHEP-TRS" evidence="28">
        <location>
            <begin position="1068"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="27">
        <location>
            <begin position="1223"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="714"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1038"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1129"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="714"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="1046"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="1129"/>
            <end position="1144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00050792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00061295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00067786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00076053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1681" mass="188980" checksum="3BEF356460871AEE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAE7</accession>
    <name>A0A452IAE7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL4m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">39S ribosomal protein L4, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IAE7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024669"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028071.1">
        <property type="protein sequence ID" value="ENSGAGP00000024669.1"/>
        <property type="gene ID" value="ENSGAGG00000018010.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000005">
        <property type="entry name" value="39S ribosomal protein L4, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_B">
        <property type="entry name" value="Ribosomal_uL4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03953">
        <property type="entry name" value="rplD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746:SF6">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL4M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00082711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="301" mass="33440" checksum="B0D6D1361A695D6B" modified="2019-05-08" version="1">MIRAGSRLWSRGAAARALATSAGRLGLTEAPVPSPHVVDAASQAEKPPLVTPSAPVLRDCCLAIPSHRTAVQAWVESLRHYEDRHVGLADLHPDVFAVMPRVDILHTVAVWQRNYKRISYAKVKTRAEVRGGGKKPWAQKGSGRARHGSIRSPLWRGGGIIHGPRGPTSYYYMLPMKVRVLGLKVALTTKLMQDNLHIVDGLDMPTCDPQYLADLARYRHWGQSVLIVDVNKMPENIMKATASLKTITLVPALGLNVHSILKHETLVLTLDTVAFLEEKLLWHDSRYTALHPFRLPYSDFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBR0</accession>
    <name>A0A452IBR0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lysosomal alpha-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Lysosomal acid alpha-mannosidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Mannosidase alpha class 2B member 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Mannosidase alpha-B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.24" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452IBR0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025196"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028685.1">
        <property type="protein sequence ID" value="ENSGAGP00000025196.1"/>
        <property type="gene ID" value="ENSGAGG00000018430.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10810">
        <property type="entry name" value="GH38N_AMII_LAM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000002">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF3">
        <property type="entry name" value="LYSOSOMAL ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="16">
        <location>
            <begin position="411"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="650" mass="72261" checksum="40B973F548281EA8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IC06</accession>
    <name>A0A452IC06_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">receptor protein serine/threonine kinase</fullName>
            <ecNumber evidence="5">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452IC06"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025223"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028714.1">
        <property type="protein sequence ID" value="ENSGAGP00000025223.1"/>
        <property type="gene ID" value="ENSGAGG00000018450.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14220">
        <property type="entry name" value="STKc_BMPR1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23612">
        <property type="entry name" value="TFP_LU_ECD_BMPR1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000018">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000001">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000055">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003605">
        <property type="entry name" value="GS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF50">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN RECEPTOR TYPE-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08515">
        <property type="entry name" value="TGF_beta_GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00467">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51256">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="20" id="KW-0464">Manganese</keyword>
    <keyword evidence="17 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="GS" evidence="24">
        <location>
            <begin position="193"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="223"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="521" mass="58928" checksum="4A404046346B1A63" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IC71</accession>
    <name>A0A452IC71_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative WW-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IC71"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025390"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028898.1">
        <property type="protein sequence ID" value="ENSGAGP00000025390.1"/>
        <property type="gene ID" value="ENSGAGG00000018564.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000299">
        <property type="entry name" value="Protein phosphatase 4 regulatory subunit 1-like (pseudogene)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000179">
        <property type="entry name" value="Serine/threonine-protein phosphatase 4 regulatory subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000358">
        <property type="entry name" value="Serine/threonine-protein phosphatase 4 regulatory subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033461">
        <property type="entry name" value="WRNPLPNID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF7">
        <property type="entry name" value="WW-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15017">
        <property type="entry name" value="WRNPLPNID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="212"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Putative WW-binding" evidence="4">
        <location>
            <begin position="457"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="877"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="958" mass="107965" checksum="51F8EC24C1916982" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICS1</accession>
    <name>A0A452ICS1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ICS1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025563"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029090.1">
        <property type="protein sequence ID" value="ENSGAGP00000025563.1"/>
        <property type="gene ID" value="ENSGAGG00000018675.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF14">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="23"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="88" mass="9786" checksum="408EA1FA1FAB9DF9" modified="2019-05-08" version="1">MDISAATMAFVNPLEKLQEEAICSICLEYMSDPVSIDCGHNFCRVCITTYCQDKGFGARGPVSCPQCRAAFCKTPLYQWPGPRQCECH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDC5</accession>
    <name>A0A452IDC5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methyl-CpG-binding domain protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Methyl-CpG-binding protein MBD4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Mismatch-specific DNA N-glycosylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with MLH1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDC5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025520"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029043.1">
        <property type="protein sequence ID" value="ENSGAGP00000025520.1"/>
        <property type="gene ID" value="ENSGAGG00000018644.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008263">
        <property type="term" value="F:pyrimidine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01396">
        <property type="entry name" value="MeCP2_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000051">
        <property type="entry name" value="Methyl-CpG-binding domain protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017352">
        <property type="entry name" value="MBD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045138">
        <property type="entry name" value="MeCP2/MBD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15074:SF7">
        <property type="entry name" value="METHYL-CPG-BINDING DOMAIN PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15074">
        <property type="entry name" value="METHYL-CPG-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038005">
        <property type="entry name" value="Methyl_CpG_bd_MBD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00391">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MBD" evidence="14">
        <location>
            <begin position="59"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="217"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="571" mass="64918" checksum="FB3BC359F9E8E12F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDQ0</accession>
    <name>A0A452IDQ0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha-actinin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDQ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025637"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029171.1">
        <property type="protein sequence ID" value="ENSGAGP00000025637.1"/>
        <property type="gene ID" value="ENSGAGG00000018720.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21214">
        <property type="entry name" value="CH_ACTN_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21216">
        <property type="entry name" value="CH_ACTN_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000005">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000005">
        <property type="entry name" value="Actinin alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000018">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000002">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000003">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014837">
        <property type="entry name" value="EF-hand_Ca_insen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08726">
        <property type="entry name" value="EFhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01184">
        <property type="entry name" value="efhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="38"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="151"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="753"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="789"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="671"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="894" mass="103862" checksum="6B3ED4B7AD1DB6B5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IE30</accession>
    <name>A0A452IE30_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CDC20/Fizzy WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IE30"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026106"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029690.1">
        <property type="protein sequence ID" value="ENSGAGP00000026106.1"/>
        <property type="gene ID" value="ENSGAGG00000019072.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF4">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 20 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="5">
        <location>
            <begin position="185"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="229"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="312"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="446"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="480" mass="53495" checksum="445B1CB7F457F83B" modified="2019-05-08" version="1">MPDSGKPTYSRFKSCIVKKLTCSVPVASSPIVTRWQQTHARNHAEQTFTECQPINLFPADGSELVRTPEKISTIPVSFQVPIKNSASEPYIITNATARCHETTYLQGGNKRRAEIAKKRKCLTQLLMIQEGGFGIPENDSIKICGNTECIWKGCQNGMHNEFTAHRVSTNSESFIPLEPEVRLHITGLRNDYYLNILDWNQENLIAIALGSAAHIWNGETHRSTEGVNLNSSLKYISSVAWIKEGTCLAVGTSDGEVQLWDIETKKRLRNMFGHLSVVGAMSWNHYILSSGSRLGLIHHHDVRISQHHVGTLCHNKQSICSLKWSTNNKLLASGSSDGLLNIWPNDPGATVHCKPLKAIPHASAVKAMNWCPWQSEILTTGGGMKDGFLRVWDVSTGKTIKTTDTKSQICSLFWLPKTNELMTGQGLPKYQMKIWKYPTLTNSTDLYGHKGRVLHLALSPDHSRIFSLAADGMACVWKYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IEJ4</accession>
    <name>A0A452IEJ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Transcription elongation factor SPT5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SPT5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IEJ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026251"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029848.1">
        <property type="protein sequence ID" value="ENSGAGP00000026251.1"/>
        <property type="gene ID" value="ENSGAGG00000019112.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06081">
        <property type="entry name" value="KOW_Spt5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06082">
        <property type="entry name" value="KOW_Spt5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06083">
        <property type="entry name" value="KOW_Spt5_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06084">
        <property type="entry name" value="KOW_Spt5_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06085">
        <property type="entry name" value="KOW_Spt5_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06086">
        <property type="entry name" value="KOW_Spt5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09888">
        <property type="entry name" value="NGN_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000013">
        <property type="entry name" value="Transcription elongation factor SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000016">
        <property type="entry name" value="Transcription elongation factor SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000017">
        <property type="entry name" value="Transcription elongation factor SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.940:FF:000003">
        <property type="entry name" value="Transcription elongation factor SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041973">
        <property type="entry name" value="KOW_Spt5_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041975">
        <property type="entry name" value="KOW_Spt5_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041976">
        <property type="entry name" value="KOW_Spt5_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041977">
        <property type="entry name" value="KOW_Spt5_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041978">
        <property type="entry name" value="KOW_Spt5_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041980">
        <property type="entry name" value="KOW_Spt5_6_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057934">
        <property type="entry name" value="KOW_Spt5_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057936">
        <property type="entry name" value="KOWx_Spt5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039385">
        <property type="entry name" value="NGN_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039659">
        <property type="entry name" value="SPT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024945">
        <property type="entry name" value="Spt5_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022581">
        <property type="entry name" value="Spt5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017071">
        <property type="entry name" value="TF_Spt5_eukaryote"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125">
        <property type="entry name" value="SUPPRESSOR OF TY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125:SF7">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23042">
        <property type="entry name" value="KOW1_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23284">
        <property type="entry name" value="KOW2_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23291">
        <property type="entry name" value="KOW4_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23290">
        <property type="entry name" value="KOW5_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23288">
        <property type="entry name" value="KOW6_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23287">
        <property type="entry name" value="KOW7_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23037">
        <property type="entry name" value="KOWx_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11942">
        <property type="entry name" value="Spt5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036945">
        <property type="entry name" value="Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01104">
        <property type="entry name" value="CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusG-like N-terminal" evidence="13">
        <location>
            <begin position="119"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="14">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="14">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="14">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="14">
        <location>
            <begin position="536"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="14">
        <location>
            <begin position="641"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="Spt5 C-terminal" evidence="15">
        <location>
            <begin position="710"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="14">
        <location>
            <begin position="972"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="611"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="685"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="623"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="709"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="772"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="795"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="815"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="834"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR036945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1025" mass="113265" checksum="B9E8AA3FA80E5D5D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IG54</accession>
    <name>A0A452IG54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Groucho/TLE N-terminal Q-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat Groucho/TLE family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000026854"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030525.1">
        <property type="protein sequence ID" value="ENSGAGP00000026854.1"/>
        <property type="gene ID" value="ENSGAGG00000019548.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005617">
        <property type="entry name" value="Groucho/TLE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009146">
        <property type="entry name" value="Groucho_enhance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814">
        <property type="entry name" value="TRANSDUCIN-LIKE ENHANCER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814:SF4">
        <property type="entry name" value="TRANSDUCIN-LIKE ENHANCER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03920">
        <property type="entry name" value="TLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01850">
        <property type="entry name" value="GROUCHOFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Groucho/TLE N-terminal Q-rich" evidence="9">
        <location>
            <begin position="13"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="557"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="599"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="681"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="166"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="243"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="757" mass="81874" checksum="9C9EA709C2C42C26" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452II55</accession>
    <name>A0A452II55_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bestrophin homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Forms chloride channels.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the anion channel-forming bestrophin (TC 1.A.46) family. Calcium-sensitive chloride channel subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452II55"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027520"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031260.1">
        <property type="protein sequence ID" value="ENSGAGP00000027520.1"/>
        <property type="gene ID" value="ENSGAGG00000019999.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000615">
        <property type="entry name" value="Bestrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021134">
        <property type="entry name" value="Bestrophin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10736">
        <property type="entry name" value="BESTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10736:SF55">
        <property type="entry name" value="BESTROPHIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01062">
        <property type="entry name" value="Bestrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0868">Chloride</keyword>
    <keyword evidence="7" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="428"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="505" mass="58129" checksum="90F7DB5A6B6665E4" modified="2019-05-08" version="1">MTVSYTLKVANAQFGGFSKLLFRWKGSIYKLLYKEFFVFVMLYAMLSITYRRLLTEEQKRVYVKVAQYCNRSTDLIPMSFVLGFYVTLIVNRWWAQYTSIPLPDQLMCVISSTVHGKDEKGRILRRSLIRYANLSSVLILRSVSTRVLKRFPTMDHLVEAGFMTQEERKKFESLHSDFNKYWIPCVWFTNLAAQARRDGRVRDDVALRLLIDELNLYRAKCSMLFHYDWISIPLVYTQVVTIAVYSFFAFCVIGRQFLEPLDPEQEQEQDQDLDLYVPLTTLLQFFFYAGWLKVAEQIINPFGEDDDDFETNKLIDRNLQVSLLSVDDMYQNLPLTGKDKYWNESTAQPPYTIATAAETLKPSFLGSTFDMRMSDDPEQSQQVEASPSLPRMHTPLLNRFLTAAASPAVSLKNFGRSNRAHHHLLRLRGEGGFPSPNPRCGEGPELVGRIEEEETEDEASEPPTPGTRLKAPNRLEASQGPRKENPQIRVRQPSDESINIDQSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJB0</accession>
    <name>A0A452IJB0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12 13">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452IJB0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027981"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031783.1">
        <property type="protein sequence ID" value="ENSGAGP00000027981.1"/>
        <property type="gene ID" value="ENSGAGG00000020322.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000031786.1">
        <property type="protein sequence ID" value="ENSGAGP00000027984.1"/>
        <property type="gene ID" value="ENSGAGG00000020322.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01927">
        <property type="entry name" value="cyclophilin_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000003">
        <property type="entry name" value="Peptidylprolyl isomerase domain and WD repeat-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000368">
        <property type="entry name" value="Peptidylprolyl isomerase domain and WD repeat-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000119">
        <property type="entry name" value="peptidylprolyl isomerase domain and WD repeat-containing protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="114"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="9">
        <location>
            <begin position="475"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="631" mass="71547" checksum="330477883DBACF9A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKG0</accession>
    <name>A0A452IKG0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc finger CCCH domain-containing protein 10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Specific regulator of miRNA biogenesis. Binds, via the C3H1-type zinc finger domains, to the binding motif 5'-GCAGCGC-3' on microRNA pri-MIR143 and negatively regulates the processing to mature microRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IKG0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028376"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032235.1">
        <property type="protein sequence ID" value="ENSGAGP00000028376.1"/>
        <property type="gene ID" value="ENSGAGG00000020590.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000003">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675">
        <property type="entry name" value="MUSCLEBLIND-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675:SF6">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="210"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="394" mass="43920" checksum="E85ACF76379B8785" modified="2019-05-08" version="1">MPDRDNYNNGSSSDEAGANSDDICRDFLRNVCKRGKRCRFRHPDISEVTNLGVRKNEFIFCHDFQNKECVRLNCRFIHGTKEDEDCYKKTGELPPRLRQKVAAGLGLSPADLPNSKEEVPICRDFLKGDCQRGAKCKFQHLQREYEYDARVIAARDPGIATTVRRYDPYDVMYDPDRYDDHDPVLKRRRVDGLHFETYEYSFTSPRTVEYRLLEEENIMLRKRLEDLKKQVNNLLATNEVLLEQNAQFRNQAKVMTLSSTATATEQTLAPTVGTVTNYNHSIAQTHTTLSSQALQPRPVTQQDLVAPAGAPAAPPTNAAPPMNPEITPLSAALAQTIAQGMAPPVSMAPVAVSVAPVAVSMAQPLGGITMSHATTPMVTYPIASQSMRITAMPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILL8</accession>
    <name>A0A452ILL8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bcl-2-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Apoptosis regulator Bcl-X</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
            <location evidence="4">Secretory vesicle</location>
            <location evidence="4">Synaptic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the Bcl-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILL8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028743"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032653.1">
        <property type="protein sequence ID" value="ENSGAGP00000028742.1"/>
        <property type="gene ID" value="ENSGAGG00000020834.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000032654.1">
        <property type="protein sequence ID" value="ENSGAGP00000028743.1"/>
        <property type="gene ID" value="ENSGAGG00000020834.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06845">
        <property type="entry name" value="Bcl-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.437.10:FF:000003">
        <property type="entry name" value="Bcl-2-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013279">
        <property type="entry name" value="Apop_reg_BclX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004725">
        <property type="entry name" value="Bcl2/BclX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020717">
        <property type="entry name" value="Bcl2_BH1_motif_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020726">
        <property type="entry name" value="Bcl2_BH2_motif_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020728">
        <property type="entry name" value="Bcl2_BH3_motif_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003093">
        <property type="entry name" value="Bcl2_BH4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00865">
        <property type="entry name" value="bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF12">
        <property type="entry name" value="BCL-2-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02180">
        <property type="entry name" value="BH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01864">
        <property type="entry name" value="APOPREGBCLX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01862">
        <property type="entry name" value="BCL2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00337">
        <property type="entry name" value="BCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00265">
        <property type="entry name" value="BH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01080">
        <property type="entry name" value="BH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01258">
        <property type="entry name" value="BH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01259">
        <property type="entry name" value="BH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50063">
        <property type="entry name" value="BH4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0770">Synapse</keyword>
    <keyword evidence="10 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Apoptosis regulator Bcl-2 family BH4" evidence="23">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="BH4" evidence="20">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00030811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="229" mass="25620" checksum="1C792CDB5FFC2E4E" modified="2019-05-08" version="1">MSNTNRELVIDFLSYKLSQRGYSWSRFEGEDEIRTDSAEEAEMASVPNGSPSWHPGASHIVNGAAGHSNSLEAHERVPATGVRQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVGRIVSWMTTYLTDHLDPWIQENGGWERFVDLYGNDAAAKSRKGQEQFNRWLLTGATLAGVLLLGSLLSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILU0</accession>
    <name>A0A452ILU0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphatidylinositol-3,4-bisphosphate 4-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.66</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1D-myo-inositol 3,4-bisphosphate + H2O = 1D-myo-inositol 3-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43388"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:83241"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Signal transduction; phosphatidylinositol signaling pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inositol 3,4-bisphosphate 4-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.66" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452ILU0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028878"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032806.1">
        <property type="protein sequence ID" value="ENSGAGP00000028878.1"/>
        <property type="gene ID" value="ENSGAGG00000020920.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016316">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000143">
        <property type="entry name" value="Type II inositol 3,4-bisphosphate 4-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039034">
        <property type="entry name" value="INPP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12187">
        <property type="entry name" value="AGAP000124-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12187:SF3">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE 4-PHOSPHATASE TYPE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="909"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="484"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="502"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="938" mass="106466" checksum="97D4B73AFC78289D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMF4</accession>
    <name>A0A452IMF4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FAM111A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IMF4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029268"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033242.1">
        <property type="protein sequence ID" value="ENSGAGP00000029268.1"/>
        <property type="gene ID" value="ENSGAGG00000021172.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14389:SF3">
        <property type="entry name" value="PROTEIN FAM111A-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14389">
        <property type="entry name" value="SI:CH1073-475A24.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="66587" checksum="E5962F068641ABB5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452INR4</accession>
    <name>A0A452INR4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sorting nexin-16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Homooligomer. Interacts with EGFR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Late endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452INR4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029498"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033494.1">
        <property type="protein sequence ID" value="ENSGAGP00000029498.1"/>
        <property type="gene ID" value="ENSGAGG00000021306.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000033496.1">
        <property type="protein sequence ID" value="ENSGAGP00000029500.1"/>
        <property type="gene ID" value="ENSGAGG00000021306.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006622">
        <property type="term" value="P:protein targeting to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07276">
        <property type="entry name" value="PX_SNX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000011">
        <property type="entry name" value="Putative sorting nexin-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037911">
        <property type="entry name" value="SNX16_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051837">
        <property type="entry name" value="SortingNexin/PXDomain-PKLike"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999">
        <property type="entry name" value="PX SERINE/THREONINE KINASE PXK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999:SF23">
        <property type="entry name" value="SORTING NEXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="18">
        <location>
            <begin position="105"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="279"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="230"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="348" mass="39257" checksum="D67B92254EAF28DD" modified="2019-05-08" version="1">MATPYVPVPIPIGSSSSNFTTNRNQRSSSFGSISTNSNSSKGQLEDSTIGNFKKMSVPEQIGSTSSICSSPLVRTKFTGVDTSIEYCTQPMEDVEQNTDSGSWEDRPSTPTILGYEVMEERAKFTVYKILVKRSPEESWVVFRRYTDFSRLNDKLKDMFPGFRLALPPKRWFKDNYNPDFLEDRQLGLQAFLQNLVAHKDIANCLAVREFLCLDDPPGPFDSLEESRAFCETLEETNYRLQKELIEKQREVESLKKLLSEKQLHIDTIEKRIRDLSLGNGKTRRMSGEESECSGEVESSAIEADQGALRDGSCSDKENHETCWSGSLAENSVPEIEVAEVAYTADEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPC1</accession>
    <name>A0A452IPC1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cortactin-binding protein-2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IPC1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029740"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033769.1">
        <property type="protein sequence ID" value="ENSGAGP00000029740.1"/>
        <property type="gene ID" value="ENSGAGG00000021471.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050719">
        <property type="entry name" value="Cortactin-Actin_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019131">
        <property type="entry name" value="Cortactin-binding_p2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23166:SF9">
        <property type="entry name" value="CTTNBP2 N-TERMINAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23166">
        <property type="entry name" value="FILAMIN/GPBP-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09727">
        <property type="entry name" value="CortBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cortactin-binding protein-2 N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="375"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="465"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="512"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="101"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="200"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="407"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="468"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="512"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="542"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="589"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="636" mass="69838" checksum="EBD6A51AD341D580" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPT6</accession>
    <name>A0A452IPT6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytosolic fatty-acid binding proteins domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IPT6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030113"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034190.1">
        <property type="protein sequence ID" value="ENSGAGP00000030113.1"/>
        <property type="gene ID" value="ENSGAGG00000021676.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19443">
        <property type="entry name" value="FABP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000001">
        <property type="entry name" value="Fatty acid-binding protein, adipocyte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00214">
        <property type="entry name" value="FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytosolic fatty-acid binding proteins" evidence="3">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="134" mass="15210" checksum="8EFDA3EC25EC2002" modified="2019-05-08" version="1">YIMCELFLGSWKLISSENFESYMKELGVGFATRKLGSLAKPSVVISTNDDIITIKTESTFKNTEISFKLGEEFEETTADDRKTKSIITLDNGALIHVQKWDGKETTIKRELVDSKLVVECTMNKVTSTRVYERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPW1</accession>
    <name>A0A452IPW1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IPW1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030144"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034222.1">
        <property type="protein sequence ID" value="ENSGAGP00000030144.1"/>
        <property type="gene ID" value="ENSGAGG00000021746.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050460">
        <property type="entry name" value="Distal-less_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327:SF72">
        <property type="entry name" value="HOMEOBOX PROTEIN NANOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="105"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="107"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="39"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="318" mass="35189" checksum="DCDA45A374466540" modified="2019-05-08" version="1">MSAHLAMPPYQPYPAGVGTGIRYGDYYWNCPAEMDNVPHKEAADADAPVPEPEEKTLHNPELSPASSSSGTRMQYTPDSATSPNTEPPSPHPAIRMGGVESGGGVKKAKSRTAFSQEQLQTLHQRFQSQKYLSPQQIRELGSALGLTYKQVKTWFQNQRMKFKRCQKETQWMEKGTCLSQVRRGFIMGSYLDLNPSYHQGCPVSASRNIQTVTNVHQSYGSSQTYGSGQSLYPFVAIEEEGFFGKAGGACSAQQAMGFFSQQKMNFYHGFPANMDYASMETEDGYHFQSASTSFPGTAGCQQYQPAWHPQGTQSNYNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPZ4</accession>
    <name>A0A452IPZ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Colony stimulating factor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IPZ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030020"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034090.1">
        <property type="protein sequence ID" value="ENSGAGP00000030020.1"/>
        <property type="gene ID" value="ENSGAGG00000021668.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030316">
        <property type="term" value="P:osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045651">
        <property type="term" value="P:positive regulation of macrophage differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008001">
        <property type="entry name" value="MCSF-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10058">
        <property type="entry name" value="MACROPHAGE COLONY STIMULATING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10058:SF0">
        <property type="entry name" value="MACROPHAGE COLONY-STIMULATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05337">
        <property type="entry name" value="CSF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="654"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="570"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="397"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="543"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="719" mass="78299" checksum="6F5364F3796BAA13" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRY1</accession>
    <name>A0A452IRY1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IRY1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030878"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035019.1">
        <property type="protein sequence ID" value="ENSGAGP00000030878.1"/>
        <property type="gene ID" value="ENSGAGG00000022207.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000084">
        <property type="entry name" value="ralBP1-associated Eps domain-containing protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000039">
        <property type="entry name" value="RalBP1-associated Eps domain-containing protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF63">
        <property type="entry name" value="RALBP1-ASSOCIATED EPS DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EH" evidence="8">
        <location>
            <begin position="10"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="8">
        <location>
            <begin position="286"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="319"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="116"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="391"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="480"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="519"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="645"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="761"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="414"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="519"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="532"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="569"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="581"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="591"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="677"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="802" mass="87621" checksum="C4C3A20513D86F44" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ISP0</accession>
    <name>A0A452ISP0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Probable ATP-dependent RNA helicase DDX49</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DEAD box protein 49</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase that plays a role in various aspects of RNA metabolism including the regulation of mRNA export and the levels of pre-ribosomal RNA. Regulates the stability and synthesis of pre-ribosomal RNA and thereby regulates cell proliferation. Also possesses antiviral activity by recognizing gammaherpesvirus transcripts in the context of lytic reactivation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX49/DBP8 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452ISP0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030890"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035031.1">
        <property type="protein sequence ID" value="ENSGAGP00000030890.1"/>
        <property type="gene ID" value="ENSGAGG00000022213.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17955">
        <property type="entry name" value="DEADc_DDX49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000892">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000993">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF25">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="33"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="218"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="444"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="466"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="496" mass="56332" checksum="9967CB6037DFF311" modified="2019-05-08" version="1">MSSFRELGLPAWLAEQAQQLGIGRPTPVQAACIPPILQGRDCMGCAKTGSGKTAAFVLPILQKLSEEPYGIFCLVVTPTRELAYQIAEQFRVLGKPLGLKDCIIVGGMDMVAQALELSHKPHVVVATPGRLADHLRSSNTFSLRKIKFLVLDEADRLLEQDCADFTKDLEVILGAVPATRQTLLFSATLTDTLKELKSLAMNKPFFWESQAEVRTVDELVQHYLLVPEKVKDAYLVHLIQTFQDEHEDWSIIIFTSTCKNCQILTMMLRRFNFPSVALHSMMKQKQRFAALARFKSSVFKILIATDVASRGLDIPTVQVVINYNTPGLPKIYIHRVGRTARAGRHGLAITLVTQYDIHLVHAIEQQIKTKLQEFSVEERAVLRILTQVNVTRRECEIKLEATDFDEKKEINKRKQMILEGKDPELEAKRKAELAKIKKKNKQFRARVQQTLQEKKQHQLKRKLQKKAQPQQRQEQRFPSPEPGFSPSVQLDQVRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUT1</accession>
    <name>A0A452IUT1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain containing 30</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IUT1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031882"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036160.1">
        <property type="protein sequence ID" value="ENSGAGP00000031882.1"/>
        <property type="gene ID" value="ENSGAGG00000022891.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052825">
        <property type="entry name" value="CCD-Prefoldin_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031476">
        <property type="entry name" value="DUF4686"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34479">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34479:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15742">
        <property type="entry name" value="DUF4686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1186"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="392"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="713"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="828"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="887"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1042"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1199"/>
            <end position="1214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1214" mass="142395" checksum="EDA9658115DBE447" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYZ8</accession>
    <name>A0A452IYZ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IYZ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033285"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037716.1">
        <property type="protein sequence ID" value="ENSGAGP00000033285.1"/>
        <property type="gene ID" value="ENSGAGG00000023638.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20868">
        <property type="entry name" value="C1_VAV2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01223">
        <property type="entry name" value="PH_Vav"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10406">
        <property type="entry name" value="SH2_Vav2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11980">
        <property type="entry name" value="SH3_VAV2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11977">
        <property type="entry name" value="SH3_VAV2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000009">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000015">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000019">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000050">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000024">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000039">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037832">
        <property type="entry name" value="PH_Vav"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035880">
        <property type="entry name" value="VAV2_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035733">
        <property type="entry name" value="VAV2_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035732">
        <property type="entry name" value="VAV2_SH3_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818:SF4">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR VAV2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818">
        <property type="entry name" value="PROTEIN VAV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 9" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 10" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="15">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="14">
        <location>
            <begin position="170"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="377"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="16">
        <location>
            <begin position="490"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="553"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="11">
        <location>
            <begin position="640"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="783"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="844" mass="97928" checksum="7A2E7AF6D51B6E79" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZ01</accession>
    <name>A0A452IZ01_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RNA-binding protein 8A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer with MAGOH. Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains CASC3, EIF4A3, MAGOH and RBM8A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the RBM8A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZ01"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033344"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037782.1">
        <property type="protein sequence ID" value="ENSGAGP00000033344.1"/>
        <property type="gene ID" value="ENSGAGG00000023769.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12324">
        <property type="entry name" value="RRM_RBM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000157">
        <property type="entry name" value="RNA-binding protein 8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008111">
        <property type="entry name" value="RNA-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033744">
        <property type="entry name" value="RRM_RBM8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45894">
        <property type="entry name" value="RNA-BINDING PROTEIN 8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01738">
        <property type="entry name" value="RNABINDINGM8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 13" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="73"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="174" mass="19857" checksum="70BBC46CD2AEF7FE" modified="2019-05-08" version="1">MADVLDLHEAGGEDFAMDEDGDESIHKLKEKAKKRKGRGFGSEEGSRARVREDYDSVEQDGDEPGPQRSVEGWILFVTGVHEEATEEDIHDKFAEYGEIKNIHLNLDRRTGYLKGYTLVEYETYKEAQAAMEGLNGQDLMGQPISVDWCFVRGPPKGKRRGGRRRSRSPDRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZ94</accession>
    <name>A0A452IZ94_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IZ94"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037853.1">
        <property type="protein sequence ID" value="ENSGAGP00000033412.1"/>
        <property type="gene ID" value="ENSGAGG00000023807.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41693">
        <property type="entry name" value="HEME-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41693:SF1">
        <property type="entry name" value="SI:CH211-243A20.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019227035" evidence="2">
        <location>
            <begin position="19"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="151" mass="18023" checksum="9BB188DB3CC76757" modified="2019-05-08" version="1">MGVLPVLLFFLLLLNCSCQKDFLNQLQRWNYREGADKVNIHGINSVTRMLEKWGNGIFWQMKHSLLSHPNTLLPEFSSLHPISEALDDLLKEVRSMKKRLEELNERLSAMSRAFSPIRFNALRGRRISSMQLRRLPFQRRRMYIRRRVQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J014</accession>
    <name>A0A452J014_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sideroflexin-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Mitochondrial serine transporter that mediates transport of serine into mitochondria, an important step of the one-carbon metabolism pathway. Mitochondrial serine is converted to glycine and formate, which then exits to the cytosol where it is used to generate the charged folates that serve as one-carbon donors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-serine(in) = L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:35031"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sideroflexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J014"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033688"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038155.1">
        <property type="protein sequence ID" value="ENSGAGP00000033688.1"/>
        <property type="gene ID" value="ENSGAGG00000023977.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140300">
        <property type="term" value="P:serine import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF20">
        <property type="entry name" value="SIDEROFLEXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="268" mass="29058" checksum="6615256A8E6D89F8" modified="2019-05-08" version="1">MGPWCGRGGLSRTNSTSHMSWRALGPFASCFPSARAELPGPRLSLLRRALPPLPTGRPLLPAVLFWQWVNQSFNAIVNYTNRSGDAPITVGQLGTAYISATTGAVVTALGLKSLTKHLPSIIGRYVPFAAVAAANCINIPLMRQRELKFGIPVTDKDGNRLGESRAAAQQAIAQVVVSRIGMAAPAMAIPPVIMNALEKRAFLKRYPWLNAPLQVGLVGFCLVFATPLCCALFPQKSSMRVSRLEPEVQALVREKNPHMEIVYFNKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J037</accession>
    <name>A0A452J037_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">WD repeat-containing protein 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Intraflagellar transport 144 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">As component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is involved in cilia function and/or assembly. Essential for functional IFT-A assembly and ciliary entry of GPCRs. Associates with the BBSome complex to mediate ciliary transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the IFT complex A (IFT-A) complex. IFT-A complex is divided into a core subcomplex composed of IFT122:IFT140:WDR19 which is associated with TULP3 and a peripheral subcomplex composed of IFT43:WDR35:TTC21B. Interacts (via C-terminal region) with IFT122 (via C-terminal region). Interacts with BBS1. Interacts with TTC25.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium</location>
            <location evidence="3">Photoreceptor outer segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000033682"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038148.1">
        <property type="protein sequence ID" value="ENSGAGP00000033682.1"/>
        <property type="gene ID" value="ENSGAGG00000023973.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030991">
        <property type="term" value="C:intraciliary transport particle A"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000242">
        <property type="entry name" value="WD repeat domain 19, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000006">
        <property type="entry name" value="WD repeat-containing protein 19 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000009">
        <property type="entry name" value="WD repeat-containing protein 19 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057855">
        <property type="entry name" value="Beta-prop_WDR19_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056168">
        <property type="entry name" value="TPR_IF140/IFT172/WDR19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040379">
        <property type="entry name" value="WDR19/dyf-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039468">
        <property type="entry name" value="WDR19_WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056170">
        <property type="entry name" value="Znf_IFT121-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14920">
        <property type="entry name" value="OSMOTIC AVOIDANCE ABNORMAL PROTEIN 1/WD REPEAT MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14920:SF0">
        <property type="entry name" value="WD REPEAT DOMAIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23389">
        <property type="entry name" value="Beta-prop_WDR19_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15911">
        <property type="entry name" value="Beta-prop_WDR19_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24762">
        <property type="entry name" value="TPR_IF140-IFT172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23145">
        <property type="entry name" value="Zf_2nd_IFT121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23146">
        <property type="entry name" value="Zf_IFT144_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR19 first beta-propeller" evidence="19">
        <location>
            <begin position="17"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="WDR19 WD40 repeat" evidence="17">
        <location>
            <begin position="361"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="IF140/IFT172/WDR19 TPR" evidence="20">
        <location>
            <begin position="662"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="domain" description="IFT121-like zinc finger" evidence="18">
        <location>
            <begin position="1277"/>
            <end position="1323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF15911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF23145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF23389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF24762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1345" mass="152203" checksum="A12AF5F218F9C785" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0M7</accession>
    <name>A0A452J0M7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 17">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with KIT.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A452J0M7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033919"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038413.1">
        <property type="protein sequence ID" value="ENSGAGP00000033919.1"/>
        <property type="gene ID" value="ENSGAGG00000024121.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd09937">
        <property type="entry name" value="SH2_csk_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11811">
        <property type="entry name" value="SH3_CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000376">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000145">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000023">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035027">
        <property type="entry name" value="Csk-like_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3 15" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 17" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="7"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="18">
        <location>
            <begin position="81"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="194"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="447" mass="50556" checksum="68122FE2B81E7B3D" modified="2019-05-08" version="1">MSTKCWLPGTQCITKHDHTKPKARELAFRKGDIVTIVEVCEQKGWYQAKHNDTGEEGLLSASSVREREAIRVDPKLSLMPWFHGKISGVEAVQALQPPEDGLFLVRESIRHPGDYVLCVSFGKEVIHYRVIYQENRLSIDASICFYNLIDMIEHYMKDQGAICTKLVKPKPKPGLKSAEEELAKAGWLLKLQHLTLGDKIGEGEFGAVLQGEYMGQKVAVKNIKCDVTAQAFLAETAAMTKVRHKNLVRLLGVILHNGLYIVMEYMSKGNLVNFLRTRGRSVLQPKQLIQFALDISQGMDYLESKKLVHRDLAARNILISEENVAKVSDFGLAKVDPKGVDATKLPVKWTAPEALKNNKFSTKSDVWSYGILLWELFSYGRAPYSKMTLKEVTEKVEKGYRMEAPEECPPSLYTLMKSCWETEPGKRPSFRKLTEKLQKELKHIGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1L4</accession>
    <name>A0A452J1L4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dynactin subunit 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Subunit of dynactin, a multiprotein complex part of a tripartite complex with dynein and a adapter, such as BICDL1, BICD2 or HOOK3. The dynactin complex is built around ACTR1A/ACTB filament and consists of an actin-related filament composed of a shoulder domain, a pointed end and a barbed end. Its length is defined by its flexible shoulder domain. The soulder is composed of 2 DCTN1 subunits, 4 DCTN2 and 2 DCTN3. The 4 DCNT2 (via N-terminus) bind the ACTR1A filament and act as molecular rulers to determine the length. The pointed end is important for binding dynein-dynactin cargo adapters. Consists of 4 subunits: ACTR10, DCNT4, DCTN5 and DCTN6. The barbed end is composed of a CAPZA1:CAPZB heterodimers, which binds ACTR1A/ACTB filament and dynactin and stabilizes dynactin. Interacts with ATP7B, but not ATP7A, in a copper-dependent manner. Interacts with ANK2; this interaction is required for localization at costameres. Interacts with N4BP2L1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Stress fiber</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the dynactin subunit 4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J1L4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034193"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038719.1">
        <property type="protein sequence ID" value="ENSGAGP00000034193.1"/>
        <property type="gene ID" value="ENSGAGG00000024291.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000038722.1">
        <property type="protein sequence ID" value="ENSGAGP00000034196.1"/>
        <property type="gene ID" value="ENSGAGG00000024291.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000038729.1">
        <property type="protein sequence ID" value="ENSGAGP00000034203.1"/>
        <property type="gene ID" value="ENSGAGG00000024291.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008603">
        <property type="entry name" value="DCTN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13034">
        <property type="entry name" value="DYNACTIN P62 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13034:SF2">
        <property type="entry name" value="DYNACTIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05502">
        <property type="entry name" value="Dynactin_p62"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="523" mass="59332" checksum="1C1D16EB4C460FAF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J2Y2</accession>
    <name>A0A452J2Y2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WD40 repeat-containing protein SMU1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Smu-1 suppressor of mec-8 and unc-52 protein homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Component of the spliceosome B complex. Interacts with IK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat SMU1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J2Y2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034763"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039352.1">
        <property type="protein sequence ID" value="ENSGAGP00000034763.1"/>
        <property type="gene ID" value="ENSGAGG00000024695.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000140">
        <property type="entry name" value="SMU1, DNA replication regulator and spliceosomal factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045184">
        <property type="entry name" value="SMU1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054532">
        <property type="entry name" value="TPL_SMU1_LisH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17814">
        <property type="entry name" value="LisH_TPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 11" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CTLH" evidence="12">
        <location>
            <begin position="26"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="196"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="246"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="289"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="331"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="470" mass="52588" checksum="8EEB1E39CD4F5E7D" modified="2019-05-08" version="1">MQYLKENSLHRALATLQEETTVSLNTVDSIESFVADINSGHWDTVLQAIQSLKLPDKTLIDLYEQVVLELIELRELGAARSLLRQTDPMIMLKQTQPERYIHLENLLARSYFDPREAYPDGSSKEKRRAAIAQALAGEVSVVPPSRLMALLGQALKWQQHQGLLPPGMTIDLFRGKAAVKDVEEEKFPTQLSRHIKFGQKSHVECARFSPDGQYLVTGSVDGFIEVWNFTTGKIRKDLKYQAQDNFMMMDDAVLCMCFSRDTEMLATGAQDGKIKVWKIQSGQCLRRFERAHSKGVTCLSFSKDSSQILSASFDQTIRIHGLKSGKTLKEFRGHSSFVNEATFTQDGHYIISASSDGTVKIWNMKTTECSNTFKSLGSTAGTDITVNSVVLLPKNPEHFVVCNRSNTVVIMNMQGQIVRSFSSGKREGGDFVCCALSPRGEWIYCVGEDFVLYCFSTVTGKLERTLTVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J3B0</accession>
    <name>A0A452J3B0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Stimulator of interferon genes protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>H(+)(in) = H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34979"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Autophagosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the STING family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J3B0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034923"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039547.1">
        <property type="protein sequence ID" value="ENSGAGP00000034923.1"/>
        <property type="gene ID" value="ENSGAGG00000024828.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061507">
        <property type="term" value="F:2',3'-cyclic GMP-AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035438">
        <property type="term" value="F:cyclic-di-GMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032481">
        <property type="term" value="P:positive regulation of type I interferon production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22658">
        <property type="entry name" value="STING_C_metazoan-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5200:FF:000001">
        <property type="entry name" value="Stimulator of interferon genes protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12100:FF:000001">
        <property type="entry name" value="Stimulator of interferon genes protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12100">
        <property type="entry name" value="Stimulator of interferon genes protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029158">
        <property type="entry name" value="STING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047191">
        <property type="entry name" value="STING_C_chordates"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038623">
        <property type="entry name" value="STING_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055432">
        <property type="entry name" value="STING_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055434">
        <property type="entry name" value="STING_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34339">
        <property type="entry name" value="STIMULATOR OF INTERFERON GENES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34339:SF1">
        <property type="entry name" value="STIMULATOR OF INTERFERON GENES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15009">
        <property type="entry name" value="STING_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23417">
        <property type="entry name" value="STING_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="STING transmembrane" evidence="15">
        <location>
            <begin position="49"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="STING ligand-binding" evidence="14">
        <location>
            <begin position="161"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF15009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="381" mass="44203" checksum="EF1BBE935293BA6A" modified="2019-05-08" version="1">FPHGILSEFMQIRKEPKVQDIGLAPSVLLAWEHCKILWQLLQSWHHLAEYVTFYFAALQIGALLRGVCNLMEEIWHVQSRYRSSWWRVVVACLSPSLRRHMLLLLISICAYMALFGDTGLQLFLNLILLCLCQLLSFAFGLQVRSPSAVEVSEICEKNNRNVAQGLAWSYYVGYLKLVLPRLKDLISEFNRANNNLLKCKETWKLHILLPVSCEIYDDLQKADSHIQYWKDLPALQLDRAGTKWRSYKQSIYTILGEDKKLYYCIVEYAPPLQSLYAMSQDESAAFSREDRLEQAQLFCRTLGEILQHSKECVGCYRLIVYEGEDDSHLLSKEILRHIRQQHLEEYTMCEGNHESTTFLSTEPSILISDSELPRPLRSDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J491</accession>
    <name>A0A452J491_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452J491"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035160"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039809.1">
        <property type="protein sequence ID" value="ENSGAGP00000035160.1"/>
        <property type="gene ID" value="ENSGAGG00000024981.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05066">
        <property type="entry name" value="PTKc_EphR_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09546">
        <property type="entry name" value="SAM_EPH-A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000041">
        <property type="entry name" value="ephrin type-A receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000019">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000045">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050449">
        <property type="entry name" value="Ephrin_rcpt_TKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF13">
        <property type="entry name" value="EPHRIN TYPE-A RECEPTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14575">
        <property type="entry name" value="EphA2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="96"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="207"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="414"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="19">
        <location>
            <begin position="704"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="776" mass="86308" checksum="2FEAB713392AAAAA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4L3</accession>
    <name>A0A452J4L3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metalloproteinase inhibitor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Tissue inhibitor of metalloproteinases 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I35 (TIMP) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J4L3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035379"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040057.1">
        <property type="protein sequence ID" value="ENSGAGP00000035379.1"/>
        <property type="gene ID" value="ENSGAGG00000025145.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051045">
        <property type="term" value="P:negative regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034097">
        <property type="term" value="P:response to cytokine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03585">
        <property type="entry name" value="NTR_TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.120:FF:000007">
        <property type="entry name" value="Metalloproteinase inhibitor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.370.10:FF:000001">
        <property type="entry name" value="Metalloproteinase inhibitor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.370.10">
        <property type="entry name" value="Tissue inhibitor of metalloproteinase-1. Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001820">
        <property type="entry name" value="TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027465">
        <property type="entry name" value="TIMP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030490">
        <property type="entry name" value="TIMP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844">
        <property type="entry name" value="METALLOPROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844:SF24">
        <property type="entry name" value="METALLOPROTEINASE INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00965">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00206">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00288">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0481">Metalloenzyme inhibitor</keyword>
    <keyword evidence="5" id="KW-0483">Metalloprotease inhibitor</keyword>
    <keyword evidence="6" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="8 12" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019563260" description="Metalloproteinase inhibitor 2" evidence="15">
        <location>
            <begin position="27"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="16">
        <location>
            <begin position="27"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared with metalloproteinase partner</note>
        </ligand>
    </feature>
    <feature type="site" description="Involved in metalloproteinase-binding" evidence="13">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="site" description="Involved in metalloproteinase-binding" evidence="13">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="27"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="29"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="39"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="154"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="159"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="220" mass="24388" checksum="E97814387685C0AA" modified="2019-05-08" version="1">MRAALPSLLAWLAMLLLWRAARPADACSCSPVHPQQAFCNADVVIRAKAVSGKEVDSGNDVYGNPIKRIQYEIKQIKMFKGPDKDIELIYTAPSSAMCGVSLDTGGKKEYLIAGKSEGNGKMHVTLCDFIVPWDSLSATQKKSLNQRYQMGCECKISRCPSIPCFVSSSDECLWTDWVTEKNINGRQAKHYACIKRSDGSCAWYRGVAPPKQEFLDIEDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J714</accession>
    <name>A0A452J714_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">L antigen family member 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. LAGE3 functions as a dimerization module for the complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the EKC/KEOPS complex composed of at least GON7, TP53RK, TPRKB, OSGEP and LAGE3; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J714"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041071.1">
        <property type="protein sequence ID" value="ENSGAGP00000036278.1"/>
        <property type="gene ID" value="ENSGAGG00000025709.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000005">
        <property type="entry name" value="L antigen family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31283">
        <property type="entry name" value="EKC/KEOPS COMPLEX SUBUNIT PCC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31283:SF5">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT LAGE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="141" mass="14478" checksum="D5B297AD2F9F03D9" modified="2019-05-08" version="1">RGSGVPPCRGGGRAVDARGSQLEVAVHGGGVPAGGSQGGRVPGGGGEGWTCARAGPGCKTPTLSIPFPSPLEAQIAHGSLAPDPEPRKGGISKELTVTESFLHVHWKADEARILRVSISSFLDHLSLVIETMEMFGPPVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7K0</accession>
    <name>A0A452J7K0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J7K0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036331"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041134.1">
        <property type="protein sequence ID" value="ENSGAGP00000036331.1"/>
        <property type="gene ID" value="ENSGAGG00000025748.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000001">
        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000008">
        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000007">
        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF23">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="45"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="309" mass="36024" checksum="F889131C5DE7E510" modified="2019-05-08" version="1">MEQVLEEVPLEVLRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLEQNAGRFDELDLSPGDPKWLDVIERDLHRQFPFHEMFVSRGGHGQQDLLRVLKAYTLHRPEEGYCQAQAPVAAVLLMHMPAEQAFWCLVQICEKYLPGYYSEKLEAIQLDGEILFSLLHRFAPLAYKHLSKQKIDPILYMTEWFMCAFSRTLPWSSVLRVWDMFFCEGVKILFRVGLVLLKHTLGSSEKLKSCQGQYETMERLRALNPKIMQEAFLVGQLAGPRLRIDSLLQPKWGEGLSLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GEX4</accession>
    <name>A0A452GEX4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Transient receptor ion channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GEX4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000086"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000098.1">
        <property type="protein sequence ID" value="ENSGAGP00000000086.1"/>
        <property type="gene ID" value="ENSGAGG00000000070.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034703">
        <property type="term" value="C:cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015279">
        <property type="term" value="F:store-operated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000157">
        <property type="entry name" value="short transient receptor potential channel 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000041">
        <property type="entry name" value="Transient receptor potential cation channel subfamily C member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013555">
        <property type="entry name" value="TRP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005459">
        <property type="entry name" value="TRPC3_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002153">
        <property type="entry name" value="TRPC_channel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00870">
        <property type="entry name" value="trp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117:SF8">
        <property type="entry name" value="SHORT TRANSIENT RECEPTOR POTENTIAL CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08344">
        <property type="entry name" value="TRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01097">
        <property type="entry name" value="TRNSRECEPTRP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01644">
        <property type="entry name" value="TRPCHANNEL3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01420">
        <property type="entry name" value="TRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="420"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="508"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="545"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="651"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="722"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Transient receptor ion channel" evidence="18">
        <location>
            <begin position="269"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="922" mass="105788" checksum="077770F0DA915E60" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFM9</accession>
    <name>A0A452GFM9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GFM9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000310"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000351.1">
        <property type="protein sequence ID" value="ENSGAGP00000000310.1"/>
        <property type="gene ID" value="ENSGAGG00000000263.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="218"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="26764" checksum="1D592AD2DBA3A402" modified="2019-05-08" version="1">MATGSMVLGLVVAPMGWVLLLAATVTPRWREFSRRPGFPWDVSFSDGLWESCVEVISVPGRACQAIPQETAISWPMQMLRALTVISVLTGVLSYSLGHVGVRWWTDSPNPKLTGTAGLLLVLSGAMYLCATSYMAYKVLENMANPQTPAGDKFWLGTCLYLGWSGGVAEILAGICLAVNFQRKEESSAGNVAAPYELPRRLGRATILSPFAELRLTPATQHRPPASAKGGPRADPMSHHTRPGLCPAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GG95</accession>
    <name>A0A452GG95_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hippocalcin-like protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the calcium-dependent regulation of rhodopsin phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GG95"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000568"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000638.1">
        <property type="protein sequence ID" value="ENSGAGP00000000568.1"/>
        <property type="gene ID" value="ENSGAGG00000000482.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000009">
        <property type="entry name" value="Visinin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF84">
        <property type="entry name" value="HIPPOCALCIN-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="146"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="191" mass="22202" checksum="40EAE0DA00728DB0" modified="2019-05-08" version="1">MGKHNSKLAPEMLDDLVRSTEFSEQELKQWYKGFLKDCPTGILNLDEFQQLYIKFFPYGDASKFAQHAFRTFDKNGDGTIDFREFICALSVTSRGSFEQKLNWAFEMYDLDGDGKITRLEMLEIIEAIYKMVGTVIMMRMNQDGLTPQQRVDKIFTKMDKDKDDQITLEEFKEAAKSDPSIVLLLQCDMQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHL2</accession>
    <name>A0A452GHL2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GHL2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001094"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001229.1">
        <property type="protein sequence ID" value="ENSGAGP00000001094.1"/>
        <property type="gene ID" value="ENSGAGG00000000883.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031510">
        <property type="entry name" value="DLEU7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36961">
        <property type="entry name" value="LEUKEMIA-ASSOCIATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36961:SF1">
        <property type="entry name" value="LEUKEMIA-ASSOCIATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15760">
        <property type="entry name" value="DLEU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019065175" evidence="2">
        <location>
            <begin position="18"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="229" mass="25617" checksum="1A987BEA1B40496C" modified="2019-05-08" version="1">MARSVPLFISLNHQLVALQTLQLLHHQQQGEDARACHPHYKLDQASAPLSIGMYGPTLHDMGLGLDAARSSRAQEQEEGRRAASKTSEEEEECGSQALSNPGEPRPKDSRQLGPARPRTLTQIAVHCMLSRIVDSTSQLVSVEQTLLLPLFQEHPFPIHLKDSIEFRNICSHMALQLEGQRFDRDLHAAHQCLRIIVEKLIDSLTVFPSDLYAIALASLRQILQNLPEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKE3</accession>
    <name>A0A452GKE3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Neprilysin</fullName>
            <ecNumber evidence="5">3.4.24.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Atriopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Enkephalinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Neutral endopeptidase 24.11</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Skin fibroblast elastase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1'.</text>
            <dbReference type="EC" id="3.4.24.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>neurotensin + H2O = neurotensin(1-10) + L-tyrosyl-L-isoleucyl-L-leucine</text>
            <dbReference type="Rhea" id="RHEA:71479"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:147362"/>
            <dbReference type="ChEBI" id="CHEBI:190705"/>
            <dbReference type="ChEBI" id="CHEBI:190707"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:71480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>neurotensin + H2O = neurotensin(1-11) + L-isoleucyl-L-leucine</text>
            <dbReference type="Rhea" id="RHEA:71475"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:147362"/>
            <dbReference type="ChEBI" id="CHEBI:190704"/>
            <dbReference type="ChEBI" id="CHEBI:190706"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:71476"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>substance P + H2O = substance P(1-7) + L-Phe-Gly-L-Leu-L-Met-NH2</text>
            <dbReference type="Rhea" id="RHEA:71467"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:190692"/>
            <dbReference type="ChEBI" id="CHEBI:190695"/>
            <dbReference type="ChEBI" id="CHEBI:190698"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:71468"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>substance P + H2O = substance P(1-9) + L-Leu-L-Met-NH2</text>
            <dbReference type="Rhea" id="RHEA:71459"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:190692"/>
            <dbReference type="ChEBI" id="CHEBI:190693"/>
            <dbReference type="ChEBI" id="CHEBI:190700"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:71460"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M13 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.11" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452GKE3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002214"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002530.1">
        <property type="protein sequence ID" value="ENSGAGP00000002214.1"/>
        <property type="gene ID" value="ENSGAGG00000001798.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097242">
        <property type="term" value="P:amyloid-beta clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08662">
        <property type="entry name" value="M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF114">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00786">
        <property type="entry name" value="NEPRILYSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18 30" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="11" id="KW-0519">Myristate</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10 30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 30" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="32">
        <location>
            <begin position="79"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="31">
        <location>
            <begin position="543"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00031127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00031362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00031486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00032584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00049470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="750" mass="85505" checksum="2FC7419BA13B1872" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKI1</accession>
    <name>A0A452GKI1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitotic spindle assembly checkpoint protein MAD2B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Mitotic arrest deficient 2-like protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GKI1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002267"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002593.1">
        <property type="protein sequence ID" value="ENSGAGP00000002267.1"/>
        <property type="gene ID" value="ENSGAGG00000001763.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.900.10:FF:000003">
        <property type="entry name" value="Mitotic spindle assembly checkpoint protein MAD2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF10">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="6">
        <location>
            <begin position="13"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="209" mass="24170" checksum="808CD4B85FFD601B" modified="2019-05-08" version="1">MTTLTRQDLNFGQVVADVLSEFLEVAVHLILYVREVYPIGIFQKRKKYNVPVQMSCHPELNQYIQDTLHCVKPLLEKNDVEKVVVVILDKEHHPVERFVFEITHPVQLVSDSLLSHVEQLLRAFILKISVCDAVLDNNPPGCTFTVLVHTREAATRNMEKIQVIKDFPWILADEQDVHMHDPRLIPLKTMTSDILKMQLYVEERAHKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKU9</accession>
    <name>A0A452GKU9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GKU9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002283"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002612.1">
        <property type="protein sequence ID" value="ENSGAGP00000002283.1"/>
        <property type="gene ID" value="ENSGAGG00000001861.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036151">
        <property type="term" value="P:phosphatidylcholine acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF7">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE LPCAT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="15">
        <location>
            <begin position="115"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="501" mass="54919" checksum="998F4238CFCA6A8E" modified="2019-05-08" version="1">MEGGSRGQDGGGAPNPFVHELRLTGAQRAKFYLLGTVLAPLRVALAFVVLFLMWPFALLQVLHRSEEELKEPLTDWRSSVSHSSVYLLSRLMFFLLGFMRIRVRGQRASRLEAPILVAAPHSTFFDPIILLPCDLPMVVSRTENLHVPVIGALLRFNQAILVSRHDPASRKKVVEEVKRRATSQGKWPQVLFFPEGTCSNKKALLKFKPGAFIAGVPIQPILVRYPNSLDSTTWAWQGPGVLKVIWLTASQLCTTVEVEFLPVYQPTAEESVNPTLYASNVQRAMAQALGVPATECELVGTLPVRAVGRLRVALEPRLWELERALRRVRWVAGGPLPDGGAGTLGCQELAHWLGLPHPEAPEELCAYFQQDMGGAVDARAVRLALQVLEGVHSPEELTRLAFELFCEVRAGLGAPCLPPEGFCAILHLLLGTPWADGAKLFAALGGAEPHHGLSIGQFQDFALRHPVYAPLFSTYLCLPVPAHRAPNPHANGLPHTKSKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GN27</accession>
    <name>A0A452GN27_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dynactin subunit 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the dynactin subunits 5/6 family. Dynactin subunit 5 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GN27"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003112"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003586.1">
        <property type="protein sequence ID" value="ENSGAGP00000003112.1"/>
        <property type="gene ID" value="ENSGAGG00000002514.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03359">
        <property type="entry name" value="LbH_Dynactin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000014">
        <property type="entry name" value="dynactin subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047125">
        <property type="entry name" value="DCTN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46126">
        <property type="entry name" value="DYNACTIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46126:SF1">
        <property type="entry name" value="DYNACTIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21711">
        <property type="entry name" value="DCTN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="187" mass="20619" checksum="ECF9C22C079D690A" modified="2019-05-08" version="1">MELSEMLYNKSEYIETASGNKVSRQSVLCGSQNIVLNGKTIVMNDCIIRGDLANVRVGRHCVVKSRSVIRPPFKKFSKGVAFFPLHIGDHVFIEEDCVVNAAQIGSYVHIGKNCVIGRRCVLKDCCKILANTVLPPETVVPPFTVFSGCPGLFSGELPECTQELMMDVTKSYYQKFLPLTQVASARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNB0</accession>
    <name>A0A452GNB0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the vestigial family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GNB0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003339"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003846.1">
        <property type="protein sequence ID" value="ENSGAGP00000003339.1"/>
        <property type="gene ID" value="ENSGAGG00000002698.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011520">
        <property type="entry name" value="Vg_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15950">
        <property type="entry name" value="TRANSCRIPTION COFACTOR VESTIGIAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15950:SF20">
        <property type="entry name" value="TRANSCRIPTION COFACTOR VESTIGIAL-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07545">
        <property type="entry name" value="Vg_Tdu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="177"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="177"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="231" mass="25433" checksum="3274008DF9F981F8" modified="2019-05-08" version="1">MEAERKNSSTLSKSKQPVKTEWGSQSVVFTYFQGDLNSVIDEHFSRALRNAKNPQDLSTKHKSEDVTLKNDSHMSTHQWNFSSHWTKSYQSSLATNTSNSDLNLSAAATDHRQASVLRNPPIQPAELWHFPSIANPHLTDSGYPRSLPDPDMVQGPMSDGKYGSFLDFLQQERCPASSQESVVKQSSNSACITGSARLQNMSQSLTPGEERKASSYQSPGNPSITPASSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNV1</accession>
    <name>A0A452GNV1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GNV1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003579"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004125.1">
        <property type="protein sequence ID" value="ENSGAGP00000003579.1"/>
        <property type="gene ID" value="ENSGAGG00000002887.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF45">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019586987" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="17"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="120" mass="13335" checksum="546F6483A1D17047" modified="2019-05-08" version="1">FLTHGSYSASIALLLGPVAGNSVTQIRASASAREGRPVKPNCQYSTTYTYYALGWYQQLPGQQPLFLVYRNERGNEYKPRSVAPRFSSELSTDVKSFTLSIEGAQWADSAVYFCALWDPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPU0</accession>
    <name>A0A452GPU0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPU0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003919"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004558.1">
        <property type="protein sequence ID" value="ENSGAGP00000003919.1"/>
        <property type="gene ID" value="ENSGAGG00000003211.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008484">
        <property type="term" value="F:sulfuric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16029">
        <property type="entry name" value="4-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.10:FF:000002">
        <property type="entry name" value="Arylsulfatase family member J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000007">
        <property type="entry name" value="Arylsulfatase family, member J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047115">
        <property type="entry name" value="ARSB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342:SF68">
        <property type="entry name" value="ARYLSULFATASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="7">
        <location>
            <begin position="50"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="573" mass="65256" checksum="D9077A7DE505BC29" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQ64</accession>
    <name>A0A452GQ64_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GQ64"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003840"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004450.1">
        <property type="protein sequence ID" value="ENSGAGP00000003840.1"/>
        <property type="gene ID" value="ENSGAGG00000003131.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018790">
        <property type="entry name" value="DUF2358"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31094">
        <property type="entry name" value="RIKEN CDNA 2310061I04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31094:SF2">
        <property type="entry name" value="RIKEN CDNA 2310061I04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10184">
        <property type="entry name" value="DUF2358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="320" mass="36050" checksum="D766B7216D739158" modified="2019-05-08" version="1">MALKFHLSLVEQQVEAARTLLLPLPGANLVGGTFLSSSEQKLSLHELLQGLEQGCPRQPTALSPLENMEASVITVDGQQEDDITVREGTGLDSASLDSLYSLFYGKPCWMPFQATAGTGTSATSGLPGNHDPSMEEHLAVMYEKLQHELPNFFLKIPDYGIYSQDVEFVNEILHMRTRGRAMYQLALALCRLVAWNYFANMRLEVLKLTQHPENWSVQARWRIVGLPFHVLMLRFYKRDKRELYRTYDAYSTFFLDSRGLIRCHRIDKLMPSQPPVVKVKKLLVATLVGLGLSEHRTSLQLLLSALAGKQQGVPVGYRDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GS48</accession>
    <name>A0A452GS48_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Myelin basic protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Myelin membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the myelin basic protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GS48"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004702"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005509.1">
        <property type="protein sequence ID" value="ENSGAGP00000004702.1"/>
        <property type="gene ID" value="ENSGAGG00000003862.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043218">
        <property type="term" value="C:compact myelin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033269">
        <property type="term" value="C:internode region of axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019911">
        <property type="term" value="F:structural constituent of myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000548">
        <property type="entry name" value="Myelin_BP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11429">
        <property type="entry name" value="MYELIN BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11429:SF0">
        <property type="entry name" value="MYELIN BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01669">
        <property type="entry name" value="Myelin_MBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00212">
        <property type="entry name" value="MYELINMBP"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00569">
        <property type="entry name" value="MYELIN_MBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="253"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="299"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="311" mass="33557" checksum="535F90E1144F901C" modified="2019-05-08" version="1">MGNHVGKRDQTAEKPIKETKVEEVEEAENKASPKSTRLTKQPSEDNEVFGEADVNQNNGTSTKSTAVTDSKGTADSKNAWNEANPADTVGRPHLIRLFSRDAPGREDNTFKDRPSESDELQTIQEDSAATSENSDLMASQKRSSFRHGSKMATASTIDHARHGSPRHRDSGLLDSLGRFFGGEKQVPRRGSGKDIHAARASHVGSMPQRSQHGRPGDDNPVVHFFKNIVSPRTPPPVQAKGRGLSLARFSWGGEGHKPGYGGSGKFSEHKSAYKGHKGSYHDGQGTLSKIFKLGGSGSRPGSRSGSPVARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GST1</accession>
    <name>A0A452GST1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromodomain Y-like protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts (via chromo domain) with histone H3K9me3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GST1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005054"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005895.1">
        <property type="protein sequence ID" value="ENSGAGP00000005054.1"/>
        <property type="gene ID" value="ENSGAGG00000004122.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18634">
        <property type="entry name" value="CD_CDY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000018">
        <property type="entry name" value="Chromodomain Y like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000012">
        <property type="entry name" value="Chromodomain Y-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051053">
        <property type="entry name" value="ECH/Chromodomain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684:SF2">
        <property type="entry name" value="CHROMODOMAIN Y-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="6">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="63"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00063797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="507" mass="56867" checksum="6AD38BC4EFC9D290" modified="2019-05-08" version="1">MASGDLYEVERIVDKRKNKKGKWEYLIRWKGYGSNEDTWEPEHHLLHCEEFIDEFNGLRVTREKRAKHGKQTSAPKFLRESRGSSVEKISHRPSESGKAKGSTHKRKRINPSLQKQKRGYTAKPAPAGDRAAKTVTYRTTPSGLQIMPLKKPHNGLQNGDAGHEKDSRHFGNGSQQQNMDLNGHEGEHDLPSMLEVNNDVPVVNGIGSSLANGGLNLHSTVKRKLDGEKDYVFDKRLRYSVRQNESNCRFRDIVVRKEDGFTHILLSSQTSDNNALTPEIMKEVRRALCNASADDSKLLLLSAVGSVFCSGLDYSYLIGRLSNDRRKESTRIAEAIRDFVKAFIQFKKPIVVAINGPALGLGASILPLCDIVWASEKAWFQTPYATIRLTPAGCSSYTFPQILGVALANEMLFCGRKLTAQEACSRGLVSQVFWPTTFSQEVMLRVKEMASCSAVVLEESKCLVRSFLKSGLEDVNEKECQMLKQLWSSSKGLDSLFSYLQDKIYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSU7</accession>
    <name>A0A452GSU7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Copine-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Copine II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes. Exhibits calcium-dependent cell membrane binding properties.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copine family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GSU7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004802"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005621.1">
        <property type="protein sequence ID" value="ENSGAGP00000004802.1"/>
        <property type="gene ID" value="ENSGAGG00000003944.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01459">
        <property type="entry name" value="vWA_copine_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000103">
        <property type="entry name" value="Copine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000042">
        <property type="entry name" value="Copine 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF3">
        <property type="entry name" value="COPINE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="134"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="544" mass="60890" checksum="860BAC7B6220C01A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GT08</accession>
    <name>A0A452GT08_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mesothelin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mesothelin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GT08"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005146"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006001.1">
        <property type="protein sequence ID" value="ENSGAGP00000005146.1"/>
        <property type="gene ID" value="ENSGAGG00000004187.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010335">
        <property type="entry name" value="Mesothelin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026664">
        <property type="entry name" value="Stereocilin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23412:SF6">
        <property type="entry name" value="MESOTHELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23412">
        <property type="entry name" value="STEREOCILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06060">
        <property type="entry name" value="Mesothelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1030" mass="114213" checksum="B91A3C89D5EA773F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTU3</accession>
    <name>A0A452GTU3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GTU3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005463"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006359.1">
        <property type="protein sequence ID" value="ENSGAGP00000005463.1"/>
        <property type="gene ID" value="ENSGAGG00000004431.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026523">
        <property type="entry name" value="PNMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048270">
        <property type="entry name" value="PNMA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048271">
        <property type="entry name" value="PNMA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095:SF17">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN MA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14893">
        <property type="entry name" value="PNMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20846">
        <property type="entry name" value="PNMA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Paraneoplastic antigen Ma-like N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Paraneoplastic antigen Ma-like C-terminal" evidence="1">
        <location>
            <begin position="182"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="344" mass="38690" checksum="E061811588C8FF21" modified="2019-05-08" version="1">MERSLLEDLCRGARIPVTKAVLMAGFPEKMEPNEIEESLDAAEILGRIKVHTCIYNRKVQGMVALCTHSKETDLNKVPKEVQVEGISWTILGAKQSPPSVPVSLEVDSLAQKLQSLMVDEKQTREELILALGGAPTPTAPSLVGWAKELGSSLKDALKLVYDSAPYKRLRSFSGVLHPSCTLIPSPGPEDDYEIWSDFTVPFVQEWEFSDADKWKGVLEALKDSQPAATVQDYLTALESVYGATDSSEDLYYRFRHTYQKRLEDLLQQVVQKQGIPIKCIDSARLDPILRGTRQDGLMLWRLQLRERAPTPPPFHKLIQEIREEEERLLQADAVLQIALAAFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GV06</accession>
    <name>A0A452GV06_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NIF system FeS cluster assembly NifU N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GV06"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005950"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006937.1">
        <property type="protein sequence ID" value="ENSGAGP00000005950.1"/>
        <property type="gene ID" value="ENSGAGG00000004838.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU N-terminal" evidence="1">
        <location>
            <begin position="39"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="100" mass="10931" checksum="C12648944282066F" modified="2019-05-08" version="1">IGVAPLDLAGSTMGNMGSLDETGKNLWYLISGCSCLWRCDETTGSSSLAMEWIKGKTVGKDFTIRNFDIAKEVCHPPVKLLCSMLAKDEIKVALTDYKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GV55</accession>
    <name>A0A452GV55_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GV55"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005866"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006845.1">
        <property type="protein sequence ID" value="ENSGAGP00000005866.1"/>
        <property type="gene ID" value="ENSGAGG00000004772.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="156" mass="17241" checksum="96CA66E358BB222A" modified="2019-05-08" version="1">MCQCHGLVPPLCHHCVLLTPVCHCHSLAPSLCHLHGPAPPMCHRHSTEPPPLCHHHSLAQPECQPQSPARPLCQPRSPARPLCHRHSLAQPECQTQSPARPLCQPQSPARPLCHRHSLAQPECQPRSLAYPLCHRHSLALLARALCHHHSLALSEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVR7</accession>
    <name>A0A452GVR7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GVR7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006074"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007077.1">
        <property type="protein sequence ID" value="ENSGAGP00000006074.1"/>
        <property type="gene ID" value="ENSGAGG00000004926.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001450">
        <property type="entry name" value="zinc finger protein 774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="207" mass="23712" checksum="FEA26028DA4D3041" modified="2019-05-08" version="1">IECGKSFTQRGYLKIHLRLHTGEKPFPCTECGKRFPSKGDLRMHQKKHTGERPFLCSECGKSFTRKVDLKVHQRKHTGERPFACTECGKSFTSKGYLKIHLGQHKGKRPFTCLECGKSYLKIHQRLHTGETPYPCSECGKRFTTKGYLKIHQKIHTDGTPFICSDCGKSFATMRYLKMHQRMTLPSKPGDDFPQKQTLCQAPLEICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GW97</accession>
    <name>A0A452GW97_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class II family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GW97"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006355"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007393.1">
        <property type="protein sequence ID" value="ENSGAGP00000006355.1"/>
        <property type="gene ID" value="ENSGAGG00000005128.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042613">
        <property type="term" value="C:MHC class II protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002504">
        <property type="term" value="P:antigen processing and presentation of peptide or polysaccharide antigen via MHC class II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05767">
        <property type="entry name" value="IgC1_MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.320.10">
        <property type="entry name" value="Class II Histocompatibility Antigen, M Beta Chain, Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014745">
        <property type="entry name" value="MHC_II_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001003">
        <property type="entry name" value="MHC_II_a_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF86">
        <property type="entry name" value="HLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DR ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00993">
        <property type="entry name" value="MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00920">
        <property type="entry name" value="MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0491">MHC II</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019237181" description="Ig-like domain-containing protein" evidence="13">
        <location>
            <begin position="26"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="116"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="258" mass="28853" checksum="3E0DC83C6DD0FCC1" modified="2019-05-08" version="1">MGAGRGVPMAQLALLTLLALPGAGAVTGDHMHSQVNFYQRTDQSQRGSGEYMQDFDHEEMFHVDLERKETVWRLPDFSKFTSFEAQGALSNMAVLKNNLEILIQRSNHTRAENVPPEVTVFPEDPVELGEPNVLICFVDKFFPPVLSVTWLKNGQEVTEGVSETDFYPRPDYSFRKFSYLPFLPSQGDFYDCRVEHWGLPEPFTKHWEAQVPTPVPETTETLVCALGLAVGIVGIIAGTILIIKGMKMNAARNPRGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXJ2</accession>
    <name>A0A452GXJ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GXJ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006864"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007964.1">
        <property type="protein sequence ID" value="ENSGAGP00000006864.1"/>
        <property type="gene ID" value="ENSGAGG00000005535.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002426">
        <property type="entry name" value="Immunoglobulin heavy variable V15-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019489397" description="Ig-like domain-containing protein" evidence="5">
        <location>
            <begin position="30"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="31"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="132"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="169" mass="18673" checksum="028690EB2F3016E5" modified="2019-05-08" version="1">ASLLHETRMKSLLLFLSLLSVQLIQSGLGTVKPGETLTLSCAVSGYSIASGYYWHWIRQPPGKGLEWMGYVYPYSGSTGYAPSLQGRVTISADTAKNQFSLQLRSLTDADTGTYYCARHTVTQSRAGTLQKEEAGFKQPSDAREAGGTEFLRDKDTHTRLNMNNPCRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYK3</accession>
    <name>A0A452GYK3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus lamina</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GYK3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007197"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008325.1">
        <property type="protein sequence ID" value="ENSGAGP00000007197.1"/>
        <property type="gene ID" value="ENSGAGG00000005781.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005652">
        <property type="term" value="C:nuclear lamina"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051664">
        <property type="term" value="P:nuclear pore localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090435">
        <property type="term" value="P:protein localization to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1260">
        <property type="entry name" value="Lamin Tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001322">
        <property type="entry name" value="Lamin_tail_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036415">
        <property type="entry name" value="Lamin_tail_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45721">
        <property type="entry name" value="LAMIN DM0-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45721:SF2">
        <property type="entry name" value="LAMIN-B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00932">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74853">
        <property type="entry name" value="Lamin A/C globular tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51841">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 5" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="9">
        <location>
            <begin position="25"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="LTD" evidence="8">
        <location>
            <begin position="447"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="379"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="570"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="22"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="228"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="436"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="571"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="590"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="601" mass="67955" checksum="646E4B080B6BB654" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYM4</accession>
    <name>A0A452GYM4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452GYM4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007232"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008362.1">
        <property type="protein sequence ID" value="ENSGAGP00000007232.1"/>
        <property type="gene ID" value="ENSGAGG00000005810.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000007">
        <property type="entry name" value="Cyclin-dependent kinase 14, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF174">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="189"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="98"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="305" mass="33435" checksum="3A486A907AAD9376" modified="2019-05-08" version="1">MIPPIYSPIYSPICMVPPTQPPHPLVCDCPPPRIFFSLWAGLPYVSCPPGLAGKSGRPWGASRSVYSFLQRRPRLGMTRSLSAHLARASRLEMVQEDVKMGSDGESDQASGTSSDEVQSPVRVRMRHSSARKISSEDINKRLSLPADIRLPEGCLEKLALNSPLFEQPLSRRLRRVSLSEIGFGKLETYVKLDKLGEGTYATVYKGKSKLTDNLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHDIIHTEKSLTLVFEYLDKDLKQYLDDCGNLINMHNVKVSAGGAGGAPGAPPGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYU9</accession>
    <name>A0A452GYU9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Golgi SNAP receptor complex member 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GYU9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007234"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008364.1">
        <property type="protein sequence ID" value="ENSGAGP00000007234.1"/>
        <property type="gene ID" value="ENSGAGG00000005811.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="17457" checksum="BEEF9956ED91023F" modified="2019-05-08" version="1">MTCVEKLNKEVLFTLFHKTRTRNHQMKLMGSRFKTNKRKFFFTQHTVSLWNSLPEDAVKAKSTIGRVDQLKYDVQHLQSALRNFQHRRYSREQQEKQREELLARTFTANVNIPCSTTWGSQTLSLLYIKVWGGDGVEDTGLDHKAGVGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZ65</accession>
    <name>A0A452GZ65_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Telomere-length maintenance and DNA damage repair domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GZ65"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007472"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008628.1">
        <property type="protein sequence ID" value="ENSGAGP00000007472.1"/>
        <property type="gene ID" value="ENSGAGG00000005983.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038980">
        <property type="entry name" value="ATM_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021668">
        <property type="entry name" value="TAN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11640">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01342">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomere-length maintenance and DNA damage repair" evidence="1">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="303" mass="35348" checksum="C5A2ACD722F0C539" modified="2019-05-08" version="1">MSLVLHELLVCCRQLENDKITERRKEVEKFKRLIRDSETINQLDHNSDYKQRKQLNWDAVFRFLQKYILKETDSIRLAKPNVSASVQASRQKKMQEISGLVKYFIRCANKRAPRLKCQELLNYVMDIVKDAPSCAIYGADCSSILLKDVLSVRKYWCEISKQQWSELLTLYCKLYLKPSRDINRVLVARIIHTLIRGCCFQTDELNSNLFCFFEKALQCARQENASAGLDHILAAINVVFSVYAVNCRMRICKLGEEILPTVLYIWTQYRPKESVKELIIQLLQLQVRVHHPKGAKTQEKGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0U7</accession>
    <name>A0A452H0U7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Metalloendopeptidase</fullName>
            <ecNumber evidence="7">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6 7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452H0U7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008200"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009441.1">
        <property type="protein sequence ID" value="ENSGAGP00000008200.1"/>
        <property type="gene ID" value="ENSGAGG00000006391.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF824">
        <property type="entry name" value="MEPRIN A SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00740">
        <property type="entry name" value="MAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="11">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="9">
        <location>
            <begin position="165"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="10">
        <location>
            <begin position="329"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="539"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="611" mass="69099" checksum="8440871B71653529" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1Z2</accession>
    <name>A0A452H1Z2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H1Z2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008644"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009938.1">
        <property type="protein sequence ID" value="ENSGAGP00000008644.1"/>
        <property type="gene ID" value="ENSGAGG00000006827.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019304220" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="92"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="203"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="287"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="462" mass="50812" checksum="BAD31B98052A8A56" modified="2019-05-08" version="1">RDWWWNVHCNLMCLELINWWSFRTQEGIAVIGMSPGGSWPKPPPSGRAEQLEREAKAGFKDCESWRVHYILFPLFDGFSCILFISLANPTPPSIFPLVPCCTKTDNAPPDTSLACLVMGYFPSPVEIKWNSGKVTQGVTTFPEVTVRNGRFTQSSLLIIPARPRQGNTYQCDVTHQGTATTESKKFPQECKTHLLPPASSLDPTIYLTKPSYEDVIKNSGHVTCLVLGYDLAASRIAWKVDGQVSFAVKTEPPKKNSNGTTSLVSSHPVSLAQWGQGTQFTCKVTTPCSGELTQDITMSNTGEEPWHLKGKDKLQVSLTLICEASGFYPAEISISWLKNNSPELKSSYNNGPVSGSVISKLEVSRSRWILGTSYSCLVAHLSSEGIIARSVNVHTGKTHLSCFIRAPKSTLHLNLNIPLLAAPHAFQSGLCLFPMCVSFDSVQMKTSFTCLLCVHSTSLLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6G8</accession>
    <name>A0A452H6G8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">3-keto-steroid reductase/17-beta-hydroxysteroid dehydrogenase 7</fullName>
            <ecNumber evidence="27">1.1.1.210</ecNumber>
            <ecNumber evidence="15">1.1.1.270</ecNumber>
            <ecNumber evidence="16">1.1.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="30">17-beta-hydroxysteroid dehydrogenase 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">3-keto-steroid reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Dihydrotestosterone oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Estradiol 17-beta-dehydrogenase 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>17beta-estradiol + NADP(+) = estrone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24616"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16469"/>
            <dbReference type="ChEBI" id="CHEBI:17263"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.62"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="24">
            <dbReference type="Rhea" id="RHEA:24618"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3-dehydro-4alpha-methylzymosterol + NADPH + H(+) = 4alpha-methylzymosterol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:36379"/>
            <dbReference type="ChEBI" id="CHEBI:1949"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:136486"/>
            <dbReference type="EC" id="1.1.1.270"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:36380"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>4alpha-methyl-5alpha-cholest-7-en-3beta-ol + NADP(+) = 4alpha-methyl-5alpha-cholest-7-en-3-one + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18409"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16495"/>
            <dbReference type="ChEBI" id="CHEBI:18378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.270"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:18411"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>4alpha-methyl-5alpha-cholest-8-en-3-one + NADPH + H(+) = 4alpha-methyl-5alpha-cholest-8-en-3beta-ol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46832"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:87050"/>
            <dbReference type="ChEBI" id="CHEBI:87051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:46833"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>5alpha-androstane-3beta,17beta-diol + NADP(+) = 17beta-hydroxy-5alpha-androstan-3-one + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16297"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16330"/>
            <dbReference type="ChEBI" id="CHEBI:18329"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.210"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:16299"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>5alpha-cholest-8-en-3-one + NADPH + H(+) = 5alpha-cholest-8-en-3beta-ol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46852"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16608"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:87056"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:46853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 3beta-hydroxysteroid + NADP(+) = a 3-oxosteroid + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:34787"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36836"/>
            <dbReference type="ChEBI" id="CHEBI:47788"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.270"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:34789"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>zymosterone + NADPH + H(+) = zymosterol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:33459"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18252"/>
            <dbReference type="ChEBI" id="CHEBI:52386"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:33460"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="17">Steroid biosynthesis; estrogen biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="26">Binds to the short form of prolactin receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.210" evidence="27"/>
    <dbReference type="EC" id="1.1.1.270" evidence="15"/>
    <dbReference type="EC" id="1.1.1.62" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A452H6G8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010117"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011610.1">
        <property type="protein sequence ID" value="ENSGAGP00000010117.1"/>
        <property type="gene ID" value="ENSGAGG00000007933.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000253">
        <property type="term" value="F:3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047024">
        <property type="term" value="F:5-alpha-androstane-3-beta,17-beta-diol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004303">
        <property type="term" value="F:estradiol 17-beta-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006703">
        <property type="term" value="P:estrogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000289">
        <property type="entry name" value="Hydroxysteroid 17-beta dehydrogenase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052834">
        <property type="entry name" value="3KSR/17beta-HSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44442">
        <property type="entry name" value="3-KETO-STEROID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44442:SF1">
        <property type="entry name" value="3-KETO-STEROID REDUCTASE_17-BETA-HYDROXYSTEROID DEHYDROGENASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00037929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00052439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00052448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00052450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00052561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00063141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00066807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00071031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00077091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00081545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00083156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00083257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="320" mass="35560" checksum="FEEBA54E7A130CCD" modified="2019-05-08" version="1">MQPVVLVTGASSGIGSALCKRLLREDAGVRLCLACRSMQKASATKAVLLAAHPTAQVTLVRLDVSSLESVLRAASEIQRRFQRLDYLFLNAGIMPNTHVNFKALISGLFSGKAVHMLSTAEGLITQEDCITADGLQAVFETNFFGHFILIRQLEPLLCCLEKPSLLIWTSSSNARKSAFSLTDYQHSKGKESYSSSKYATDLASVALNKHFNKQGLYSSVVCPGLVMTNLTYGILPSFFWKLLMPIMWIIRFFTKTYTLTPYNGAEAQVWLFRQKPESLNALMKYHSCISPMGKNYVESRKVNTAKTLKSNLLLMVFCTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HA71</accession>
    <name>A0A452HA71_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HA71"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011617"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013312.1">
        <property type="protein sequence ID" value="ENSGAGP00000011617.1"/>
        <property type="gene ID" value="ENSGAGG00000008973.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901224">
        <property type="term" value="P:positive regulation of non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31450:SF4">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31450">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 19 LRRC19 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15176">
        <property type="entry name" value="LRR19-TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019517592" description="LRRCT domain-containing protein" evidence="5">
        <location>
            <begin position="24"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="256"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="6">
        <location>
            <begin position="166"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="356" mass="40187" checksum="B540427641DACD37" modified="2019-05-08" version="1">GSKIICNFLCLYCFSLSLQQVTCEESAKNYSSIPTSLSKNVTKLYLSNNNITLNDTDKKVLQLFINLTELYLNENAITVLHNNTFCNLSELTILDISSNYISTIEQAAFKGLNQLSILYLNHNKISQLDSDTITSLKKLMILSLQDNLLKYFDVKASFKLITLNGNPWNCSCGLLSLQKWLNASNVTMEHENDTLCAYPDVWNKSSIKTAPIQKADCDSRRGSIAITTPSTSAILTSSLNSSNNNIRSSPPLGKTWVFLLGVLVFVLSTSLLIFIAIKFPLWYVYLISYNHRRLKEYEPEMFDKEFTTDLSTFPSISNTNEQDSIAVFEQTHTFVLDEDGFIEDKYIDDPGLTEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAF4</accession>
    <name>A0A452HAF4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HAF4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011705"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013410.1">
        <property type="protein sequence ID" value="ENSGAGP00000011705.1"/>
        <property type="gene ID" value="ENSGAGG00000009040.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042612">
        <property type="term" value="C:MHC class I protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002474">
        <property type="term" value="P:antigen processing and presentation of peptide antigen via MHC class I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000204">
        <property type="entry name" value="Major histocompatibility complex, class I-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF242">
        <property type="entry name" value="MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I-RELATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0490">MHC I</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="161"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="310" mass="34649" checksum="051C72D7CCFD354A" modified="2019-05-08" version="1">MERGLPACLGSHLKGAGRGRTQQHKWEPHFSGEGERSSKEKNYWAPCSAALLRFSERLLLTLSCVSGFHIIQTMFGCDLREDGIIQGFYQDSYDGRDFLTFDKETMTWVAADIGAQITKRRWHAEVDDNQGWTHFLEETCISWLRSALEFGKETLQRRVRPTARVSDRSSHDGLTTLSCKVSGFYPRDITVTWLKNGENRQQETYSEGILPSGDGTYQTWVTMEIDPTIRAHYSCWVEHESLLEPLSISWAPKNSLIPIVAGVITAVILVGVIIGVVIWKKKCPGRKGDGYAVAQADDQGSSGSDMAAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HB01</accession>
    <name>A0A452HB01_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452HB01"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011921"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013654.1">
        <property type="protein sequence ID" value="ENSGAGP00000011921.1"/>
        <property type="gene ID" value="ENSGAGG00000009190.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07877">
        <property type="entry name" value="STKc_p38alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000063">
        <property type="entry name" value="Mitogen-activated protein kinase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000769">
        <property type="entry name" value="Mitogen-activated protein kinase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038784">
        <property type="entry name" value="MAPK14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008352">
        <property type="entry name" value="MAPK_HOG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01773">
        <property type="entry name" value="P38MAPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0346">Stress response</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="24"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR638784-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="360" mass="41501" checksum="56DF339F7C9F2407" modified="2019-05-08" version="1">MSQERPKFYRQELNKTVWEVPERYQNLSPVGSGAYGSVCSAFDRKTGLRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFTPAKSLEEFNDVYLVTHLMGADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGPELLKKISSESARNYIQSLSYMPKMNFENVFIGANPLAVDLLEKMLVLDTDKRITAAEALAHGYFSQYHDPDDEPVADPYDQSFESRELEIEEWKSLTYDEVVSFVPPPLDQEEMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HB78</accession>
    <name>A0A452HB78_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000012011"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013755.1">
        <property type="protein sequence ID" value="ENSGAGP00000012011.1"/>
        <property type="gene ID" value="ENSGAGG00000009253.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004487">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF7">
        <property type="entry name" value="BIFUNCTIONAL METHYLENETETRAHYDROFOLATE DEHYDROGENASE_CYCLOHYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="2">
        <location>
            <begin position="22"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="108" mass="12015" checksum="FC428A0ADBF4125A" modified="2019-05-08" version="1">GKTKKCRPQHLTXLYSDEATIISGTQLAKQVYKEVQRDVESWMSLGNRRPHLTVILVGDNPASHTYVRNKMKAATALIVSAVECPISRPQHLTTTPHNSSNSFALSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBX7</accession>
    <name>A0A452HBX7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000012277"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014053.1">
        <property type="protein sequence ID" value="ENSGAGP00000012269.1"/>
        <property type="gene ID" value="ENSGAGG00000009441.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000014061.1">
        <property type="protein sequence ID" value="ENSGAGP00000012277.1"/>
        <property type="gene ID" value="ENSGAGG00000009441.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14365">
        <property type="entry name" value="CUE_N4BP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013899">
        <property type="entry name" value="DUF1771"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056718">
        <property type="entry name" value="DUF7816"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056719">
        <property type="entry name" value="DUF7817"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056720">
        <property type="entry name" value="DUF7818"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052772">
        <property type="entry name" value="Endo/PolyKinase_Domain-Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041801">
        <property type="entry name" value="N4BP2_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535:SF1">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08590">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25124">
        <property type="entry name" value="DUF7816"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25125">
        <property type="entry name" value="DUF7817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25126">
        <property type="entry name" value="DUF7818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01162">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SMR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="3">
        <location>
            <begin position="45"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Smr" evidence="2">
        <location>
            <begin position="1761"/>
            <end position="1840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="941"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1000"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1041"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1399"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="946"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1041"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1405"/>
            <end position="1418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1840" mass="207253" checksum="CD03059F7C27416A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCL9</accession>
    <name>A0A452HCL9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Intraflagellar transport protein 27 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Putative GTP-binding protein RAY-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Rab-like protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Small GTPase-like component of the intraflagellar transport (IFT) complex B that promotes the exit of the BBSome complex from cilia via its interaction with ARL6. Not involved in entry of the BBSome complex into cilium. Prevents aggregation of GTP-free ARL6. Required for hedgehog signaling. Forms a subcomplex within the IFT complex B with IFT25. Its role in intraflagellar transport is mainly seen in tissues rich in ciliated cells such as kidney and testis. Essential for male fertility, spermiogenesis and sperm flagella formation. Plays a role in the early development of the kidney. May be involved in the regulation of ureteric bud initiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HCL9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012531"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014358.1">
        <property type="protein sequence ID" value="ENSGAGP00000012531.1"/>
        <property type="gene ID" value="ENSGAGG00000009623.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001095">
        <property type="entry name" value="Intraflagellar transport protein 27 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="186" mass="21073" checksum="B51C96A8C160475A" modified="2019-05-08" version="1">MVKLAAKCILTGDSAVGKSALAQMFCNDGAHFQKNYTLTTGVELLVKTISVPETSDSVELFLFDSAGKELFSEMMEKLWEQPNVLCIVYDVTNEQSFNNCAKWLEKLRAQTLGMHLPGVLVGNKTDLIGRRVVEQKWAKEWADIHGLQYCETSVKEMENIEAPFHILANSFHRLYREKVETFHSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HD89</accession>
    <name>A0A452HD89_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HD89"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014627.1">
        <property type="protein sequence ID" value="ENSGAGP00000012761.1"/>
        <property type="gene ID" value="ENSGAGG00000009791.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="72" mass="8308" checksum="E6072E85B52BB2E6" modified="2019-05-08" version="1">MPQLNSQGGFSAFSGVVTFQTYFFPPEEPEYSRQRFYLFNELTILHDEREAIQAPAFVFTKARLEGRGRKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDA5</accession>
    <name>A0A452HDA5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Clathrin-coated pit</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HDA5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012805"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014679.1">
        <property type="protein sequence ID" value="ENSGAGP00000012805.1"/>
        <property type="gene ID" value="ENSGAGG00000009831.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018808">
        <property type="entry name" value="Muniscin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF8">
        <property type="entry name" value="F-BAR DOMAIN ONLY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10291">
        <property type="entry name" value="muHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0168">Coated pit</keyword>
    <keyword evidence="3" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD" evidence="7">
        <location>
            <begin position="497"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="141"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="288"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="329"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="375"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="774" mass="83769" checksum="FD77623723805009" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDT5</accession>
    <name>A0A452HDT5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HDT5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012968"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014862.1">
        <property type="protein sequence ID" value="ENSGAGP00000012968.1"/>
        <property type="gene ID" value="ENSGAGG00000009953.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12242">
        <property type="entry name" value="RRM_SLIRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000494">
        <property type="entry name" value="28 kDa ribonucleoprotein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034152">
        <property type="entry name" value="SLIRP_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="52"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="145" mass="16669" checksum="E22CCB06E365CE0D" modified="2019-05-08" version="1">MTTSHPPLPRPEGVKADGRLGSDWLSGKRSEVRIHSSAFKMAATRASSRRVFELFVSRIHWTLATKELRDYFAQFGTVRRCMLPFDKETGFHKGYCWVGFSSEEGLRNALQKESHILDGIKLQVRQQKPRSFRSQYTNEGETDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEB4</accession>
    <name>A0A452HEB4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">eIF-2-alpha kinase GCN2</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Eukaryotic translation initiation factor 2-alpha kinase 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">GCN2-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="23">Homodimer; homodimerization is important for kinase activation by uncharged tRNAs. Interacts with GCN1; this interaction stimulates EIF2AK4/GCN2 kinase activity and is impaired by IMPACT upon a variety of stress conditions, such as amino acid depletion, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Interacts with DNAJC3; this interaction inhibits EIF2AK4/GCN2 kinase activity during endoplasmic reticulum (ER), hypothermic and amino acid-starving stress conditions. Interacts with MAP3K20; activates EIF2AK4/GCN2 kinase activity in response to moderate ribotoxic stress.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013161"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015066.1">
        <property type="protein sequence ID" value="ENSGAGP00000013161.1"/>
        <property type="gene ID" value="ENSGAGG00000010083.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14012">
        <property type="entry name" value="PK_eIF2AK_GCN2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23823">
        <property type="entry name" value="RWD_GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14046">
        <property type="entry name" value="STKc_EIF2AK4_GCN2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000338">
        <property type="entry name" value="Eukaryotic translation initiation factor 2-alpha kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2-alpha kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000353">
        <property type="entry name" value="Eukaryotic translation initiation factor 2-alpha kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000057">
        <property type="entry name" value="eukaryotic translation initiation factor 2-alpha kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000308">
        <property type="entry name" value="Eukaryotic translation initiation factor 2-alpha kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000031">
        <property type="entry name" value="Eukaryotic translation initiation factor 2-alpha kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016255">
        <property type="entry name" value="Gcn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024435">
        <property type="entry name" value="HisRS-related_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF136">
        <property type="entry name" value="EIF-2-ALPHA KINASE GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12745">
        <property type="entry name" value="HGTP_anticodon2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000660">
        <property type="entry name" value="Ser/Thr_PK_GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="18" id="KW-0010">Activator</keyword>
    <keyword evidence="11 28 29" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0338">Growth arrest</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="8 28 29" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="16" id="KW-0346">Stress response</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="RWD" evidence="32">
        <location>
            <begin position="23"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="31">
        <location>
            <begin position="302"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="31">
        <location>
            <begin position="597"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="140"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="666"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="764"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="143"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="30">
        <location>
            <begin position="242"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="679"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="711"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="777"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="27">
        <location>
            <position position="850"/>
        </location>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <begin position="603"/>
            <end position="611"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28 29">
        <location>
            <position position="626"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00065481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00073598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00076428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00083151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR000660-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR000660-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1652" mass="188622" checksum="24F6F0D5458D062B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HG41</accession>
    <name>A0A452HG41_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apolipoprotein C-III</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Apolipoprotein C3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the apolipoprotein C3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HG41"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013819"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015823.1">
        <property type="protein sequence ID" value="ENSGAGP00000013819.1"/>
        <property type="gene ID" value="ENSGAGG00000010532.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042627">
        <property type="term" value="C:chylomicron"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.90.10">
        <property type="entry name" value="Apolipoprotein CIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008403">
        <property type="entry name" value="Apo-CIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038195">
        <property type="entry name" value="Apo_CIII_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14225">
        <property type="entry name" value="APOLIPOPROTEIN C-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14225:SF0">
        <property type="entry name" value="APOLIPOPROTEIN C-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05778">
        <property type="entry name" value="Apo-CIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0162">Chylomicron</keyword>
    <keyword evidence="8" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0850">VLDL</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="134" mass="14960" checksum="EA8A985C6D67ED06" modified="2019-05-08" version="1">MAWSPGGCHLGQSQRIIRSRATLLLLSLPGGSSITRQSSFPCGPCCRLPAAAVLVLPLLPAAAPREEETIVKKVQDYVHQVAQTAKDALTKVQESEVAQQARDWVNMRAEDIHQFWDVLKDKFSTLWEQQPPWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGE5</accession>
    <name>A0A452HGE5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prefoldin subunit 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Represses the transcriptional activity of MYC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGE5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013934"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015954.1">
        <property type="protein sequence ID" value="ENSGAGP00000013934.1"/>
        <property type="gene ID" value="ENSGAGG00000010617.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990113">
        <property type="term" value="P:RNA polymerase I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990114">
        <property type="term" value="P:RNA polymerase II core complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990115">
        <property type="term" value="P:RNA polymerase III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23157">
        <property type="entry name" value="Prefoldin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000004">
        <property type="entry name" value="Probable prefoldin subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00308">
        <property type="entry name" value="PfdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00293">
        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674:SF2">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00057914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="155" mass="17251" checksum="291D975980F07874" modified="2019-05-08" version="1">MAQQVNITELTLPQLEVLKNQLDQEVEFLSSSIAQLKVVQTKYVEAKDCLNVLSKSNEGKELLVPLTSSMYVPGKLSDVGHVLVDVGTGYYVEKTADNARDFFKRKIDFLTKQMEKIQPALQEKHAMKQAVMEMMSQKIQQLTAMGASQAAATKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHY5</accession>
    <name>A0A452HHY5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HHY5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014465"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016548.1">
        <property type="protein sequence ID" value="ENSGAGP00000014465.1"/>
        <property type="gene ID" value="ENSGAGG00000010994.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028074">
        <property type="entry name" value="PHTB1_GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028073">
        <property type="entry name" value="PHTB1_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026511">
        <property type="entry name" value="PTHB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055364">
        <property type="entry name" value="PTHB1_CtH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055363">
        <property type="entry name" value="PTHB1_hp_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055362">
        <property type="entry name" value="PTHB1_pf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20991">
        <property type="entry name" value="PARATHYROID HORMONE-RESPONSIVE B1 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20991:SF0">
        <property type="entry name" value="PROTEIN PTHB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14727">
        <property type="entry name" value="PHTB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23339">
        <property type="entry name" value="PTHB1_CtH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14728">
        <property type="entry name" value="PTHB1_GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23338">
        <property type="entry name" value="PTHB1_hp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23337">
        <property type="entry name" value="PTHB1_pf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PTHB1 N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="PTHB1 GAE" evidence="3">
        <location>
            <begin position="435"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="PTHB1 platform" evidence="4">
        <location>
            <begin position="530"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="PTHB1 hairpin" evidence="5">
        <location>
            <begin position="640"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="PTHB1 C-terminal helix bundle" evidence="6">
        <location>
            <begin position="744"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="890" mass="100010" checksum="E349ABDC7E4F86F0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HID2</accession>
    <name>A0A452HID2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Formin GTPase-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HID2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014664"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016766.1">
        <property type="protein sequence ID" value="ENSGAGP00000014664.1"/>
        <property type="gene ID" value="ENSGAGG00000011130.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF16">
        <property type="entry name" value="DISHEVELED-ASSOCIATED ACTIVATOR OF MORPHOGENESIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formin GTPase-binding" evidence="1">
        <location>
            <begin position="46"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="102" mass="11882" checksum="A969F65F7523D352" modified="2019-05-08" version="1">MAPRKRSGRGISFIFCCFRSNDHPEITYRLRNDSNFALQTMEPALPMPPVEELDVMFSELVDELDLTDKHREAMFALPAEKKWQIYCSKKKTPLLVSVEEMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJQ3</accession>
    <name>A0A452HJQ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HJQ3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015148"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017305.1">
        <property type="protein sequence ID" value="ENSGAGP00000015148.1"/>
        <property type="gene ID" value="ENSGAGG00000011437.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="56"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="108" mass="11876" checksum="BFAF5C5B0F6596E2" modified="2019-05-08" version="1">NEDLPGTDSPAPLWLVGVMDLLGETRGSLFVPTPWCFQDALSSWGWDSVDCSPPTVLSVGCLLFQGPEEWALLDPAQRALYRAIMQENYENVTSLGKDSCPLCSWKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKU0</accession>
    <name>A0A452HKU0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1</fullName>
            <shortName evidence="9">Oligosaccharyl transferase subunit DAD1</shortName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the DAD/OST2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HKU0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015554"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017768.1">
        <property type="protein sequence ID" value="ENSGAGP00000015554.1"/>
        <property type="gene ID" value="ENSGAGG00000011733.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003038">
        <property type="entry name" value="DAD/Ost2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02109">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005588">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="120" mass="12596" checksum="9238F91FD2323E7F" modified="2019-05-08" version="1">MSGAAGSGGGSVGSVVRRFLAEYSSGTASRLKVLDAYLLYVLLSGALQFGYCLGVGTFPFNSFLSGFISAVGSFILGVCLRIQINPQNKGDFQGISPERAFADFLFASTILHLVVINFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLX2</accession>
    <name>A0A452HLX2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HLX2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015943"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018218.1">
        <property type="protein sequence ID" value="ENSGAGP00000015943.1"/>
        <property type="gene ID" value="ENSGAGG00000012000.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071787">
        <property type="term" value="P:endoplasmic reticulum tubular network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2480:FF:000001">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046964">
        <property type="entry name" value="RTN1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799:SF4">
        <property type="entry name" value="RETICULON-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="8">
        <location>
            <begin position="44"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU210713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="232" mass="25342" checksum="25432E67268848E4" modified="2019-05-08" version="1">MADPATQSPYISSSAGGGPSEPGTTPIPAGGRDCKGTSSQSCAVHDLIFWRDVKKTGFVFGTSLIMLLSLAAFSVISVVSYLILALLSVTISFRVYKSVIQAVQKSEEGHPFKAYLDLDITLSSEAFHNYVNDAMVHVNRALKLIIRLFLVEDLVDSLKLAVVMWLMTYVGAVFNGITLLILGELLIFGIPVVYEKYKTQIDRYVGIIRDQTKSIIAKIQAKLPGAVKKKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMF9</accession>
    <name>A0A452HMF9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">S-formylglutathione hydrolase</fullName>
            <ecNumber evidence="3 8">3.1.2.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 8">Serine hydrolase involved in the detoxification of formaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>S-formylglutathione + H2O = formate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="3.1.2.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the esterase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.12" evidence="3 8"/>
    <dbReference type="AlphaFoldDB" id="A0A452HMF9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016175"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018469.1">
        <property type="protein sequence ID" value="ENSGAGP00000016175.1"/>
        <property type="gene ID" value="ENSGAGG00000012141.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018738">
        <property type="term" value="F:S-formylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000002">
        <property type="entry name" value="S-formylglutathione hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014186">
        <property type="entry name" value="S-formylglutathione_hydrol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02821">
        <property type="entry name" value="fghA_ester_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061:SF0">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0719">Serine esterase</keyword>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR614186-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="329" mass="37077" checksum="EF6CD1B1590FBAB9" modified="2019-05-08" version="1">MLRAALLCPPRFRLLRLRRPPAPPSPRPAPEVPYFCLKIERSQPAEIMALKQISSNKCFEGFQKVFEHDSIELKCKMRFGIYLPPKAEIGKCPVLYWLSGLTCTEQNFITKSGFHQAAAEHGLIVVAPDTSPRGCNIEGEEESWDFGTGAGFYVDATEEPWKTNYRMYSYIKDELPKLIKANFPADSERMSIFGHSMGGHGALILALKNPGKYKSVSAFAPICNPVQCPWGKKAFGGYLGSDLTKWEAYDATNLVKSYPDSHLDILIDQGKDDQFLSAGQLLPDNFIAACTERKIPVVFRLQQGYDHSYFFITTFINDHIRHHAKYLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HP98</accession>
    <name>A0A452HP98_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Annexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Zymogen granule membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HP98"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016827"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019211.1">
        <property type="protein sequence ID" value="ENSGAGP00000016827.1"/>
        <property type="gene ID" value="ENSGAGG00000012570.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034704">
        <property type="term" value="C:calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042589">
        <property type="term" value="C:zymogen granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055074">
        <property type="term" value="P:calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000003">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000004">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000022">
        <property type="entry name" value="Annexin A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002391">
        <property type="entry name" value="ANX4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF28">
        <property type="entry name" value="ANNEXIN A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00200">
        <property type="entry name" value="ANNEXINIV"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7 11" id="KW-0041">Annexin</keyword>
    <keyword evidence="4 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 11" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="321" mass="35979" checksum="A1C4F4513727E76B" modified="2019-05-08" version="1">MAKAGNRGTIREALGFSAEEDAQKLRKAMKGLGTDEDAIIDIVAKRSASQRQQILITYKSTIGRDLIGDLKSELSGNFEKVIIGLMTSITLYDVQELKRAMKGAGTDEGCLIEILATRSSEEIQRINETYHRQYGTTLEKDIVSDTSSMFRRVLVSLATGNRDQGNYVDAGLAQQDAQCLYEAGEKKWGTNEGQFLTILCSRNTNHLLKVFDEYRRIAKKDITESIKSEMSGDLEDALLAVVKCIRNKHAYFAERLYGSMKGLGTDDDTLIRVMVSRCEIDMLDIRHEFKRMYGKSLYSFIQGDTSGDYRKVLLLLCGGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQQ8</accession>
    <name>A0A452HQQ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription factor AP-2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQQ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017351"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019795.1">
        <property type="protein sequence ID" value="ENSGAGP00000017351.1"/>
        <property type="gene ID" value="ENSGAGG00000012913.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004979">
        <property type="entry name" value="TF_AP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013854">
        <property type="entry name" value="TF_AP2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812:SF13">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2-EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03299">
        <property type="entry name" value="TF_AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01748">
        <property type="entry name" value="AP2TNSCPFCT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor AP-2 C-terminal" evidence="8">
        <location>
            <begin position="208"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="434" mass="46791" checksum="C42ED6F878131E4F" modified="2019-05-08" version="1">MLVHTYSAMDRPDGLNGSSTGGRLSQLSSLNQAAYSSAPPLCHTPASDFQPPYFPPPYPQPPMPYSQSQEAGYPHLGDPYSAINPIHQHQQPGWHSQRTRQEEAGLLSQTHRALSLDPRREYPGVPRLLHGLADGGHGLGDGPLGLHGLGHPSLEDIQAVDEAGLNLLDQSVIKKVPIPSKANGTTISALSMNKDSLIGGVTNPNEVFCSVPGRLSLLSSTSKYKVTVGEVQRRLSPPECLNASLLGGVLRRAKSKNGGRCLRERLEKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYVCETEFPAKAAAEYLCRQHSDPSELHTRKNMLLATKQICKEFADLMAQDRSPLGNSRPSLILEPGVQSCLTHFSLITHGFGGPAICAALTAFQNYLVESLKGLDKMFMNSTGNGHAAGDSKTSEKEVKHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQY2</accession>
    <name>A0A452HQY2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cysteine protease</fullName>
            <ecNumber evidence="12">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylserine + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phospho-L-serine</text>
            <dbReference type="Rhea" id="RHEA:67576"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="Rhea" id="RHEA-COMP:17326"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172942"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67577"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase C54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452HQY2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017463"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019914.1">
        <property type="protein sequence ID" value="ENSGAGP00000017463.1"/>
        <property type="gene ID" value="ENSGAGG00000012972.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF36">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12" id="KW-0645">Protease</keyword>
    <keyword evidence="8 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="14">
        <location>
            <begin position="104"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="490" mass="54993" checksum="93D23F72B2CFC785" modified="2019-05-08" version="1">MNSVSPASVQYVSQDELRHSDGRKLFNPRAGGQDGSCNGLFPGGAQSRQPNEPDEVDKIKNKLLSAWNNVKYGWTVRTKTNFSKLSPIYLFGHGYCFEVEEDVERFQKDFASRIWLTYRREFQQLEGTMWTTDCGWGCMLRSGQMILAQGLLMHFLGRNWTWPDALFTTSLVEMDAMKPISPVRPGSAGLQQLPALAVDRDSDPWGIWAPLRPLSLQELEKEHSHRTIVSWFADHPRAPFGIHQLVELGRSSGKKAGDWYGPSIAAHIVRKAVEGCSEASGLAVYVSQDCTVYKGDVARLVSGSDGRTVPDPGARRKAVIILVPMRLGGETLNPVYVDCVKELLKLELCIGIIGGKPKHSLYFVGYQDDFLLYLDPHYCQPFVDTTKENFPLESFHCSSPRKMAFSKMDPSCTIGFYARDGQELDTLCSELTRVLNSSSAKEKYPMFTVTEGQAQEYGLAELCSRFSQQTVQLPRRRKPKVATSDEFVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HT80</accession>
    <name>A0A452HT80_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DET1 partner of COP1 E3 ubiquitin ligase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HT80"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018311"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020867.1">
        <property type="protein sequence ID" value="ENSGAGP00000018311.1"/>
        <property type="gene ID" value="ENSGAGG00000013551.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019138">
        <property type="entry name" value="De-etiolated_protein_1_Det1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13374:SF3">
        <property type="entry name" value="DET1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13374">
        <property type="entry name" value="DET1 HOMOLOG DE-ETIOLATED-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09737">
        <property type="entry name" value="Det1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="64011" checksum="2A80BE081E7D674C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUI9</accession>
    <name>A0A452HUI9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HUI9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018762"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021386.1">
        <property type="protein sequence ID" value="ENSGAGP00000018762.1"/>
        <property type="gene ID" value="ENSGAGG00000013880.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03593">
        <property type="entry name" value="CLECT_NK_receptors_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033992">
        <property type="entry name" value="NKR-like_CTLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47498">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 2 MEMBER L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47498:SF1">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 2 MEMBER L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="80"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="185" mass="20424" checksum="2A159D9F5DEB88E1" modified="2019-05-08" version="1">MLQRPAARPPIPSPDTNSPYPPEGHKFRAGLGSSRKPALGDLTGRNVMVCCCFLAVNSSSGKGSYGSPPPEACPADWLYHQRKCYYFSETEKDWDSSWSFCSSHNSSLTVIENQQELNFIMKITKQDPWIGLHKTGEEFHWVNGTPLDRNLLAVKGSGECAYIESDVVSSSGCSLTRGWVCKKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYH5</accession>
    <name>A0A452HYH5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYH5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020229"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023047.1">
        <property type="protein sequence ID" value="ENSGAGP00000020229.1"/>
        <property type="gene ID" value="ENSGAGG00000014919.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015370">
        <property type="term" value="F:solute:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF63">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="6" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="417"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="473"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="508"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="551"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="591"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="633" mass="70303" checksum="2F428A18C6FBD4CE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZ51</accession>
    <name>A0A452HZ51_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZ51"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020463"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023312.1">
        <property type="protein sequence ID" value="ENSGAGP00000020463.1"/>
        <property type="gene ID" value="ENSGAGG00000015073.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF51">
        <property type="entry name" value="AMINOTRANSFERASE CLASS I_CLASSII DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795">
        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="4">
        <location>
            <begin position="62"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="434"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="445"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="481" mass="54372" checksum="FF583D8ADF73717C" modified="2019-05-08" version="1">MSSGGAGGNGRVAALSARGSRIASGARGLLDKGFDLYMADPFDRERNPRGILNLGTSENKLCYDLIQERLTRPDMNYLEPHLLQYSDPQGIENFREEIAKFLTEYAKAPKALNPEHIVVMNGCCAVFATLSTVLCDPGDGYLIPTPYYGGINSKTWLYGGIQPVHVPLFSEVTDEESHPFQLTVEKLETALQRAKEQGIRVRALILINPHNPLGDIYPAQLLKECLEFAHRYQLHVIIDEIYMLSVYDDTTFTSVLSLDCLPDPERTHFMWGFSKDFGMCGIRVGVLYARSHEIRKAVNQLAVFHGCPGPVQYVLSQFLSDRDWLDNVYFPTNKKRLREAQNILVDGLTKIGIPVLKSSGGLYVWADFRKFLKSQTFEAEMELWWKFLDEKLLISPGKAFCCYEPGWFRMVFSDSIDKIYLGIQRLEQMLQTETAEPVANKTSAAEDELSNLEDETSVNHTNTPLDEVSEAYSDMKKIVVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I084</accession>
    <name>A0A452I084_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone-lysine N-methyltransferase EZH2</fullName>
            <ecNumber evidence="2">2.1.1.356</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60292"/>
            <dbReference type="Rhea" id="RHEA-COMP:15535"/>
            <dbReference type="Rhea" id="RHEA-COMP:15548"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.356"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.356" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452I084"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020898"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023799.1">
        <property type="protein sequence ID" value="ENSGAGP00000020898.1"/>
        <property type="gene ID" value="ENSGAGG00000015364.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140951">
        <property type="term" value="F:histone H3K27 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19218">
        <property type="entry name" value="SET_EZH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000001">
        <property type="entry name" value="Putative histone-lysine N-methyltransferase EZH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026489">
        <property type="entry name" value="CXC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045318">
        <property type="entry name" value="EZH1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048358">
        <property type="entry name" value="EZH1/2_MCSS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021654">
        <property type="entry name" value="EZH1/EZH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044439">
        <property type="entry name" value="EZH2_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041343">
        <property type="entry name" value="PRC2_HTH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041355">
        <property type="entry name" value="Pre-SET_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747:SF3">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE EZH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21358">
        <property type="entry name" value="Ezh2_MCSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11616">
        <property type="entry name" value="EZH2_WD-Binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18118">
        <property type="entry name" value="PRC2_HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18264">
        <property type="entry name" value="preSET_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51633">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CXC" evidence="16">
        <location>
            <begin position="504"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="15">
        <location>
            <begin position="613"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="181"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="342"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="182"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="360"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="747" mass="85505" checksum="F96794DEBAC4416E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0R5</accession>
    <name>A0A452I0R5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000021054"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023976.1">
        <property type="protein sequence ID" value="ENSGAGP00000021054.1"/>
        <property type="gene ID" value="ENSGAGG00000015459.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="32"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="138"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="273" mass="29060" checksum="694AB766FE314423" modified="2019-05-08" version="1">MAESHSSPQRPLYPLPPAEWTCRRCLIKSVVPQVQLVQSGLGVVKSGQTLILTCAVSGVSITDSRYSWDWFRQPLGAGLECMAGIYPHGGNTNYAPSLQSRVTVSADTAKNQLSSLTAADTGTYYCARDTVTQSKVGVAQKEETVSKQPSEAXGGRGGAHCSTPGSATGPAPTPPVPTLSPEPALPSLPLPQEHPAHHKTADREVRGGRGRLLSLFPVLCYHKGSVLSLQLWLLPHWAFPGEHCSTSWLLGFASHPRTTGVTGFPTHQTALPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0S4</accession>
    <name>A0A452I0S4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000021103"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024038.1">
        <property type="protein sequence ID" value="ENSGAGP00000021103.1"/>
        <property type="gene ID" value="ENSGAGG00000015498.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="69"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="198" mass="22475" checksum="DA86901BBF612858" modified="2019-05-08" version="1">MRGTSINNLLPSHIHPHLSPLGSDTLLAILTKMGLGIFHLSLLRVLLLDFLFPHKASXWTCCPMGLKHFQSSCYYFSRDIMNWGDSETNCTGMGSHLVVINKGTEQVTLWGLDSLGNQAKEGQWLWVDQTPFVQHLKLIPICISHVSYRFWRQNEPSNGNMENCAVMHIETKANRKNWNDIPCSTKVYRICETAATSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1P5</accession>
    <name>A0A452I1P5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452I1P5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021389"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024362.1">
        <property type="protein sequence ID" value="ENSGAGP00000021389.1"/>
        <property type="gene ID" value="ENSGAGG00000015704.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000318">
        <property type="entry name" value="ATP-dependent RNA helicase DDX19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000357">
        <property type="entry name" value="ATP-dependent RNA helicase DDX19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="119"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="152"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="333"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="57"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="119"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="506" mass="57043" checksum="14F3A1413F045204" modified="2019-05-08" version="1">MSLFPRRRHCGSWSRACERPACRHHRTMATDSWALAVDEQEAAAESLSNLHLKEAKTKPDSNGAVVKTDDNAEKTEDEEKEDRAAQSLLNKLIRSNLVDTTNQVEVLQRDPTSPLYSVKSFEELRLKPQLLQGVYAMGFNRPSKIQENALPMMLAEPPQNLIAQSQSGTGKTAAFVLAMLSRVEPGNKYPQCLCLSPTYELALQTGKVIEQMGKFHPELSLAYAVRGNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIATQGHQDQSIRIQRMLPRDCQMLLFSATFEDSVWKFAQKVVPDPNIIKLKREEETLDTIKQYYVLCNDRGEKFQALCNIYGAITIAQAMIFCHTRKTAGWLAGELSKEGHQVALLSGEMMVEQRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRTGRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIEKLDTDDLDEIEKIAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1P9</accession>
    <name>A0A452I1P9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mTERF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I1P9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021394"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024368.1">
        <property type="protein sequence ID" value="ENSGAGP00000021394.1"/>
        <property type="gene ID" value="ENSGAGG00000015709.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006393">
        <property type="term" value="P:termination of mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000003">
        <property type="entry name" value="transcription termination factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15437:SF1">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15437">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="394" mass="45700" checksum="94DC6B2CAF7C8C86" modified="2019-05-08" version="1">MLRGIANSATHMFTFSLTRSQHCKPLLTLSSLWKNIKDASFLQHFSYTTDIKSKVENKRTVENLYRLAVDIKKIRRLKEWVLFQDAAYVKETANTLREMGADKTAIANILERCPEAILRTPAEINSQRDLWQSVCQNEKQLVKLIEQFPESFFTVEHHENQKSNVQFFQELGLKNNIISRFFTSAPDIFYKPVEKNKHIVETLQRMYLNLGGSNANMKIWLLKLLSQNPFILLNSTTAVQENLEFLQKKDFTDSQVLQLLSRLKGFIFNLSPDTMQRSMFFSKDVFKCSDQELKELVLKCPALLYNSVLVLEERLRGLLKEGISVDQIKETPMVLELTTQIVQYRIKKLAALGYNIKNGNLENLNGTKRDFEATYCRIQAKRERPMFNPVAPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2S0</accession>
    <name>A0A452I2S0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Saposin B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I2S0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021835"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024874.1">
        <property type="protein sequence ID" value="ENSGAGP00000021835.1"/>
        <property type="gene ID" value="ENSGAGG00000016031.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044194">
        <property type="term" value="C:cytolytic granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061844">
        <property type="term" value="P:antimicrobial humoral immune response mediated by antimicrobial peptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038847">
        <property type="entry name" value="Granulysin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15541:SF2">
        <property type="entry name" value="GRANULYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15541">
        <property type="entry name" value="GRANULYSIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saposin B-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="85" mass="9718" checksum="9BC219565F94859A" modified="2019-05-08" version="1">QGWHVQEQALSVRCTICTNILKKLKSMVGDDPDEDSIVEAEDKLCGVVGRSLQRLCRYVMKKYKPKITEALQDGYHSETRFLGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3E7</accession>
    <name>A0A452I3E7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD-dependent protein deacylase</fullName>
            <ecNumber evidence="5">2.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Regulatory protein SIR2 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">NAD-dependent protein deacylase. Catalyzes the NAD-dependent hydrolysis of acyl groups from lysine residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-acetyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-acetyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:43636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="ChEBI" id="CHEBI:83767"/>
            <dbReference type="EC" id="2.3.1.286"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the sirtuin family. Class II subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452I3E7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022057"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025131.1">
        <property type="protein sequence ID" value="ENSGAGP00000022057.1"/>
        <property type="gene ID" value="ENSGAGG00000016184.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01409">
        <property type="entry name" value="SIRT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01967">
        <property type="entry name" value="Sirtuin_ClassII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026587">
        <property type="entry name" value="Sirtuin_class_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003738">
        <property type="entry name" value="PRK05333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="7">
        <location>
            <begin position="40"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5 6">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="263"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="289"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="317" mass="35214" checksum="31F1A2E4CBD8D4FD" modified="2019-05-08" version="1">MSIYCALKVPEGCRALGLHHYRSHSVAKASPNLAFVPASPPPDAVEVEALQRFVSHSQRLLVMTGAGISTESGIPDYRSEGVGLYARTDRRPIQHAEFVRSASARQRYWARNFVGWPQFSSHQPNAAHLALRNWEKLGKLHWLVTQNVDALHTKAGSQRVMELHGCTHRVLCLGCGEKTPRSELQKHFEALNPTWRAEAHGIAPDGDVFLTEEQVHNFRVPACSRCGGILKPDVTFFGDTVSQEKVDFVHKRLAESDSMLVAGSSLQVYSGYRFALAAHEKKLPIVIINIGPTRSDHFASMKLNSRCGELLPLIVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I425</accession>
    <name>A0A452I425_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I425"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025401.1">
        <property type="protein sequence ID" value="ENSGAGP00000022287.1"/>
        <property type="gene ID" value="ENSGAGG00000016372.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000183">
        <property type="entry name" value="Myelin-oligodendrocyte glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF149">
        <property type="entry name" value="BG-LIKE ANTIGEN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="84" mass="10087" checksum="154C66C7D942417C" modified="2019-05-08" version="1">MEVRWYRSQFYSLVHLYRDGDNQNERQMPEYQGRTEFLRDDIANGSVALRIRHVRPSDEGQYKCFFRSSVFHEESLLELKVAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I472</accession>
    <name>A0A452I472_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cell membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I472"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022334"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025450.1">
        <property type="protein sequence ID" value="ENSGAGP00000022334.1"/>
        <property type="gene ID" value="ENSGAGG00000016395.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000001">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000004">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1190:FF:000001">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF278">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN TYPE 2 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 15" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 15" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="13 15" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 15" id="KW-0813">Transport</keyword>
    <keyword evidence="7 15" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="17">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="SCN5A-like C-terminal IQ motif" evidence="18">
        <location>
            <begin position="361"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="415"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="415"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="442"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="468" mass="53608" checksum="3B3EF94DD44BB92D" modified="2019-05-08" version="1">MILICLNMVTMMVETDDQSDEMQYNLWWINLVFIVLFTGECILKLISLRYYYFTIGWNIFDFVVVILSIVGMFLADLIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGEPDCDPYKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEYSKLSDFAASLDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDTLRIQMEDRFMAANPSKVSYEPITTTLKRKQEEVSAVIIQRAFRRYLLKQKIKKVSCMFNQVKDKDEDDLPIKEDMIIDKLNENSTPEKTDMTPSTTSPPSYDSVTKPDKEKYEKDKSEKEDKGKDIRESKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6G5</accession>
    <name>A0A452I6G5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I6G5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023212"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026447.1">
        <property type="protein sequence ID" value="ENSGAGP00000023212.1"/>
        <property type="gene ID" value="ENSGAGG00000017018.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051016">
        <property type="entry name" value="Diverse_Substrate_AcTransf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545">
        <property type="entry name" value="DIAMINE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545:SF51">
        <property type="entry name" value="THIALYSINE N-EPSILON-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14672" checksum="2262AA8337816BB8" modified="2019-05-08" version="1">AMSYVVRPWALRDLKDVMRLIRVSSGGKRHTIVGYQFHYFRYCTWTGRIIFGEDLYVMPEFRGRGPFGSSVLIYVLLRRFMSANWNHPATALYEKLGAVDVTARDNWVIWHIEGQAVQEMAQQATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I744</accession>
    <name>A0A452I744_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAM69 protein-kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DIPK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I744"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026739.1">
        <property type="protein sequence ID" value="ENSGAGP00000023457.1"/>
        <property type="gene ID" value="ENSGAGG00000017200.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020519">
        <property type="entry name" value="DIPK2A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022049">
        <property type="entry name" value="FAM69_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32073:SF8">
        <property type="entry name" value="DIVERGENT PROTEIN KINASE DOMAIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32073">
        <property type="entry name" value="GH11358P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12260">
        <property type="entry name" value="PIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019194683" description="FAM69 protein-kinase domain-containing protein" evidence="5">
        <location>
            <begin position="33"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="FAM69 protein-kinase" evidence="6">
        <location>
            <begin position="209"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="431" mass="49259" checksum="99383F23868529CD" modified="2019-05-08" version="1">MGRWMYYFCSNVLGLLILLQFLTLNWSPSLEATVFPSSVPHVKPSYSFGRTFLGLDKCNACIGTSICKKFFKEEIRFDNWLASHLKLPPNYLPSYSGNYTDDAKSWRLVEISRLTTKYQHDQADKRICASLSKAKSCSIEHVLRKTERFQKWLKAKRLTPDLVQGLPSPMLRCPSQRLLDRIVRRYAEVPDAGSIYMDHLTDRDKLRLLYTLSVNAHPILLQIFPEAEGWPFPKYLGSCGRLIVTASTRPLKEFYSSPPEVAADLALQLLSIVNSMRNNDMNYFFYFVNVDAGTFGIFNNGLLFIRDASTLGIIDKQEGLQLTDGQQEYKDIFSCLASDCQSAFPSCNSVTEKQSLIMVCQELLPKLLKGKFLQPVQEKIDRFLRLCADSFRADQEVSDSAGKLIELLKSLQSCDSHFAYRYPDCKYSDKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I750</accession>
    <name>A0A452I750_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homeobox protein Nkx-3.2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Bagpipe homeobox protein homolog 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NK-3 homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I750"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023463"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026745.1">
        <property type="protein sequence ID" value="ENSGAGP00000023463.1"/>
        <property type="gene ID" value="ENSGAGG00000017204.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000225">
        <property type="entry name" value="NK3 homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF34">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX-3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="151"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="153"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="17"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00081047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="280" mass="30700" checksum="C11CE115CD0BF48E" modified="2019-05-08" version="1">MAVRGGNALTPFSIQAILNKKEERAQHLAGRPPPATPTAPSPASPAGWDSDSALSDDHEGERRSEEEGTGVQQGPPSSTARSREAPGRGRPAALVSGSGGGISQGSRIRIVMGSRRTHRKLAVERYRPCEDGGSKCEKLLAGEEEQAAPKPRKKRSRAAFSHAQVFELERRFNHQRYLSGPERADLAASLKLTETQVKIWFQNRRYKTKRRQMAADLLASAPAAKKVAVKVLVRDDQRQYHPGEMLRPPSLLSLQPSYYYPYYCLPGWALSTCTAAAGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7T9</accession>
    <name>A0A452I7T9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DMRT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I7T9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023698"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026995.1">
        <property type="protein sequence ID" value="ENSGAGP00000023698.1"/>
        <property type="gene ID" value="ENSGAGG00000017352.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007548">
        <property type="term" value="P:sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1040.10:FF:000001">
        <property type="entry name" value="doublesex- and mab-3-related transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1040.10">
        <property type="entry name" value="DM DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001275">
        <property type="entry name" value="DM_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036407">
        <property type="entry name" value="DM_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026607">
        <property type="entry name" value="DMRT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322">
        <property type="entry name" value="DOUBLESEX AND MAB-3 RELATED TRANSCRIPTION FACTOR DMRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322:SF122">
        <property type="entry name" value="DOUBLESEX- AND MAB-3-RELATED TRANSCRIPTION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00751">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00301">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82927">
        <property type="entry name" value="Cysteine-rich DNA binding domain, (DM domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS40000">
        <property type="entry name" value="DM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50809">
        <property type="entry name" value="DM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DM" evidence="8">
        <location>
            <begin position="90"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="DM" evidence="6">
        <location>
            <begin position="90"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="32"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="521" mass="58719" checksum="9EC30FB15381A422" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I847</accession>
    <name>A0A452I847_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Choline dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.99.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>choline + A = betaine aldehyde + AH2</text>
            <dbReference type="Rhea" id="RHEA:17433"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15710"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.1.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.1" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A452I847"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023816"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027123.1">
        <property type="protein sequence ID" value="ENSGAGP00000023812.1"/>
        <property type="gene ID" value="ENSGAGG00000017425.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000027125.1">
        <property type="protein sequence ID" value="ENSGAGP00000023814.1"/>
        <property type="gene ID" value="ENSGAGG00000017425.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000027128.1">
        <property type="protein sequence ID" value="ENSGAGP00000023816.1"/>
        <property type="gene ID" value="ENSGAGG00000017425.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000027132.1">
        <property type="protein sequence ID" value="ENSGAGP00000023819.1"/>
        <property type="gene ID" value="ENSGAGG00000017425.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00529">
        <property type="reaction ID" value="UER00385"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008812">
        <property type="term" value="F:choline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019285">
        <property type="term" value="P:glycine betaine biosynthetic process from choline"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011533">
        <property type="entry name" value="BetA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01810">
        <property type="entry name" value="betA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002550">
        <property type="entry name" value="PRK02106.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0274">FAD</keyword>
    <keyword evidence="3 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="9">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="10">
        <location>
            <begin position="316"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="154"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="613" mass="68439" checksum="3C70A3B41BEB4DD1" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9W6</accession>
    <name>A0A452I9W6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I9W6"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027686.1">
        <property type="protein sequence ID" value="ENSGAGP00000024322.1"/>
        <property type="gene ID" value="ENSGAGG00000017777.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="243" mass="24199" checksum="C6EFBB88B7577357" modified="2019-05-08" version="1">GCGPLRPRPRACPSAWGCGPLQSHPGTCPAAWGCDSLRTCAGACPSAWGCSLLWPRAGACPSAWGCGPLQSHPGTCPAAWGCDSLRTRAGACPSVWGCGPLWPRAGGLSLCLGLWPPPAPPRGLSLCLGLWPPLALPVGLSLCLGLWPPPAPRRGPVLLPGAVAPSGPTRDPVPLPGAVAPSGPAQGPVPLPGAVVPSGPVLGACPSAWGCDPLRPRPGACPSAWGCGPLWPRAGGLSICLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB42</accession>
    <name>A0A452IB42_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Perilipin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the perilipin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IB42"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024953"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028403.1">
        <property type="protein sequence ID" value="ENSGAGP00000024953.1"/>
        <property type="gene ID" value="ENSGAGG00000018244.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010890">
        <property type="term" value="P:positive regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.340">
        <property type="entry name" value="Flagellar protein FliS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.170">
        <property type="entry name" value="Perilipin, alpha-beta domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004279">
        <property type="entry name" value="Perilipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024">
        <property type="entry name" value="PERILIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024:SF51">
        <property type="entry name" value="PERILIPIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03036">
        <property type="entry name" value="Perilipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036881">
        <property type="entry name" value="PAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109775">
        <property type="entry name" value="Mannose-6-phosphate receptor binding protein 1 (Tip47), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="410" mass="44881" checksum="654B7716999C01EC" modified="2019-05-08" version="1">MQKPDTTPHKASLSQPEPQPQGNSATPLQQPRINVVSRVANMPLVSSALTAKGVKTMTEVIISCAQPVLTKLKPQIAAANKFACKGLDKLEEKLPILHQPTDKVLLDAKELVTGTRDAMNSRVTEVLDKTMEAAKSTVTSSMSTVMGSRLGLGAVSRAEAVQEKSADVVDHSLPITDDELAKLAASVKGFEVTSVEQQQKYFVHLGSLSTELCPWTAGRHTGEVRLLHQSMQENLSQIQHTIDLIQNVKQSVGKELHQDHQKLYQLWLEWTKKELAVIDKGSARPEQVDTLVLVMSWRITQQLQTMCLNLAISIQGPPASIQDKMQQAHHNLHKVHAVFSKASSFQGLSTSILTCSKQNLLKGGDRLENPASILVVLHANGTYQTCSSHLSVMYCIASHLIKAVCKGIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBH5</accession>
    <name>A0A452IBH5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger AN1-type containing 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IBH5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025093"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028570.1">
        <property type="protein sequence ID" value="ENSGAGP00000025093.1"/>
        <property type="gene ID" value="ENSGAGG00000018356.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4770:FF:000001">
        <property type="entry name" value="Zinc finger AN1-type containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1110.10:FF:000001">
        <property type="entry name" value="Zinc finger AN1-type containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050652">
        <property type="entry name" value="AN1_A20_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634:SF27">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN 6 ISOFORM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01754">
        <property type="entry name" value="zf-A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00259">
        <property type="entry name" value="ZnF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51036">
        <property type="entry name" value="ZF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="A20-type" evidence="8">
        <location>
            <begin position="8"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="9">
        <location>
            <begin position="141"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="41"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="22449" checksum="4E107AB07D5ED59A" modified="2019-05-08" version="1">MAQETNQTQVPMLCTTGCGFYGSPRTNGMCSVCYKEYLQRRQSSGRISPPASGPSNSPAASDSIPGQGTEGDPTPEDVKTSTQTPVTHQMTAMSISREDTSSETVGYISTDETSSASSSSETLLEISQNSAEGEKTSEKPKQKKNRCFTCRKKIGLTGFDCRCGNLFCAIHRYSDMHSCPYDYKAEAAEKIRKENPIIVAEKIQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBS7</accession>
    <name>A0A452IBS7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histamine H3 receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IBS7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025216"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028707.1">
        <property type="protein sequence ID" value="ENSGAGP00000025216.1"/>
        <property type="gene ID" value="ENSGAGG00000018443.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016907">
        <property type="term" value="F:G protein-coupled acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004969">
        <property type="term" value="F:histamine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007197">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15048">
        <property type="entry name" value="7tmA_Histamine_H3R_H4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000138">
        <property type="entry name" value="histamine H3 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003980">
        <property type="entry name" value="Histamine_H3_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF199">
        <property type="entry name" value="HISTAMINE H4 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01471">
        <property type="entry name" value="HISTAMINEH3R"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="172"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="29"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="210"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="348" mass="39622" checksum="C943F4DAD973320D" modified="2019-05-08" version="1">CRRGAPGGPSPAPLSWILMVLIALITVLGNGLVVLAFLVNKDLRHRSNYLFLNLAISDFLVGAFCIPLYIPYFLTGKWMFGRFLCKLWLVVDYLMCTASAFSIVLISYDRFLSVTKAVTYRIQQGMTSKTVVKMVAVWVFAFLIYGPAILIWDRVAGYSIILDGECNAEFYYNWYFLLCASTFEFFTPCISVAYFNMHIYRDIQKRKRNRLQNVSSPDSETEDSLSTSRTQVQAKTRSKLNRDKKIAKSLAIIVCVFAICWAPYSLLMIIRAACCGECVNEFLYEITFWLLWLNSSVNPFLYPLCHAGFRKAFMKILCPKSLTIFPHCQKAPLHTTCVCIMVLGLVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDA1</accession>
    <name>A0A452IDA1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Synaptotagmin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Synaptotagmin I</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Chromaffin granule membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDA1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025785"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029330.1">
        <property type="protein sequence ID" value="ENSGAGP00000025785.1"/>
        <property type="gene ID" value="ENSGAGG00000018819.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042584">
        <property type="term" value="C:chromaffin granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031045">
        <property type="term" value="C:dense core granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08385">
        <property type="entry name" value="C2A_Synaptotagmin-1-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08402">
        <property type="entry name" value="C2B_Synaptotagmin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000007">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000016">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF249">
        <property type="entry name" value="SYNAPTOTAGMIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="107"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="238"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="396" mass="44934" checksum="1A70749B36E23540" modified="2019-05-08" version="1">MANKMSSNSTTAPSALMTTAALTTAKPGFIDGIINKIPLPKWALIAIAVAAGLLLILFLICIIKCCCCKKKHKKKEKLNLQNINGSTTASLVQPDMEDLESGLENEKRGRLQYSLEYNFRSQEMKVGVKQAADLKAMDSGGTSDPYVIVYLTSDMRKKYESKVYRKTLNPVFNETFTFQIPQAEMSESTLVMQIYDFNRFAKHDIIGEVRLPLGDFDLQHVIEEWQELTAASKMEQERLGEICFSLRYVPSTSKLTVVILEAKKLKRMDSSGLSDPFVKVQLILNKKKWKKKKTGVKKSTLSPYFNEAFNFEVPFNQIQNIDLVISVWDHDKVTKNQQIGKLFLGCRATGNQLRHWSDMLANPRRPIAQWHSLQPVEEVDNALRLKSHFKLPLPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDP5</accession>
    <name>A0A452IDP5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Rhodopsin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 22">Membrane</location>
            <topology evidence="1 22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="21">Contains one covalently linked retinal chromophore.</text>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDP5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025632"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029165.1">
        <property type="protein sequence ID" value="ENSGAGP00000025632.1"/>
        <property type="gene ID" value="ENSGAGG00000018676.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016037">
        <property type="term" value="P:light absorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000018">
        <property type="entry name" value="Rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000732">
        <property type="entry name" value="Rhodopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019477">
        <property type="entry name" value="Rhodopsin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10413">
        <property type="entry name" value="Rhodopsin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00579">
        <property type="entry name" value="RHODOPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 21 22" id="KW-0157">Chromophore</keyword>
    <keyword evidence="11 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 22" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13 19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="2 22" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="12 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21 22" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="4 22" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="14 22" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0844">Vision</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="102"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="24">
        <location>
            <begin position="54"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in the conformation switch to the active conformation" evidence="17">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(retinylidene)lysine" evidence="21">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-palmitoyl cysteine" evidence="20">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-palmitoyl cysteine" evidence="20">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="19">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="19">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="110"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600732-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR600732-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600732-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR600732-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU004951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="355" mass="39931" checksum="635D70391B5782D3" modified="2019-05-08" version="1">MNGTEGINFYVPFSNKTGLVRSPFEYPQYYLAEPWKYRIVCCYIFFLIFTGLPINFLTLLVTFKHKKLRQPLNYILVNLAVADLFMACFGFTVTFSTAWNGYFIFGPIGCAVEGFFATLGGQVALWSLVVLAIERYIVVCKPMGNFRFSATHALMGISFTWAMAFSCAAPPLFGWSRYIPEGMQCSCGPDYYTLNPDYHNESYVVYMFAVHFVIPVVVIFFSYGRLICKVREAAAQQQESASTQKAEREVTRMVILMVLGFLMAWTPYALVAFWIFTNKGVDFSATLMSVPAFFSKSSSLYNPVIYVLMNKQFRNCMITTICCGKNPFGDDDVSSTVSQSKTEVSSVSSSQVSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IEA1</accession>
    <name>A0A452IEA1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000026033"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029609.1">
        <property type="protein sequence ID" value="ENSGAGP00000026033.1"/>
        <property type="gene ID" value="ENSGAGG00000019016.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028104">
        <property type="entry name" value="DUF4553"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14931">
        <property type="entry name" value="GENE 340-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14931:SF2">
        <property type="entry name" value="LIGAND DEPENDENT NUCLEAR RECEPTOR COREPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15090">
        <property type="entry name" value="DUF4553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="658"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="973"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1343"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1404"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1455"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="434"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="581"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="879"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="911"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="985"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1063"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1093"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1103"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1150"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1353"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1406"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1490"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1530"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1632"/>
            <end position="1647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1647" mass="177987" checksum="17CD565289E17A79" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFE9</accession>
    <name>A0A452IFE9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Speriolin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IFE9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026668"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030310.1">
        <property type="protein sequence ID" value="ENSGAGP00000026668.1"/>
        <property type="gene ID" value="ENSGAGG00000019417.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026715">
        <property type="entry name" value="SPATC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029384">
        <property type="entry name" value="Speriolin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22192">
        <property type="entry name" value="SPERIOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22192:SF1">
        <property type="entry name" value="SPERIOLIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15059">
        <property type="entry name" value="Speriolin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Speriolin C-terminal" evidence="1">
        <location>
            <begin position="194"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="339" mass="38587" checksum="2195123C47CC6862" modified="2019-05-08" version="1">MTDDNEWMKTIQNENAYLKNQVRLLRENYELRSLLSQHYENSNEGQIVTSYPAPVYPNACSPRQGGKENLDDLVLNAPDLLSQPQPSSLGEFISSNPLSISNKEGAQAQLVFPGYFKTSEPFSPGIPPRKPVPLINSTRDIVGEKMSLDLEEMHRKKKVWFSESPGGDEPRKPHTYYLNEDRSISGYYKKNGRIVGEIAFQLDRRILAYVFPGVTRLYGYTVSNIPEKIKQASMKCLDGSMDEKKHRAMMQQYLSLTARLEKIGYNRDVHPVFSEFLINTYGILKQRPDVHSSPLHSSPADLRKVVIDIVPSKFLGDTLLLLNCLCELSKEDSRPLFAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFI9</accession>
    <name>A0A452IFI9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Succinyl-CoA:3-ketoacid-coenzyme A transferase</fullName>
            <ecNumber evidence="9">2.8.3.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 3-oxo acid + succinyl-CoA = a 3-oxoacyl-CoA + succinate</text>
            <dbReference type="Rhea" id="RHEA:24564"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35973"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.8.3.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:24565"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>acetoacetate + succinyl-CoA = acetoacetyl-CoA + succinate</text>
            <dbReference type="Rhea" id="RHEA:25480"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="2.8.3.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:25481"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the 3-oxoacid CoA-transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.3.5" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452IFI9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026705"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030351.1">
        <property type="protein sequence ID" value="ENSGAGP00000026705.1"/>
        <property type="gene ID" value="ENSGAGG00000019449.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00929">
        <property type="reaction ID" value="UER00894"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008260">
        <property type="term" value="F:succinyl-CoA:3-oxo-acid CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046952">
        <property type="term" value="P:ketone body catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000001">
        <property type="entry name" value="Succinyl-coa:3-ketoacid-coenzyme a transferase subunit b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000002">
        <property type="entry name" value="Succinyl-CoA:3-ketoacid-coenzyme A transferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012792">
        <property type="entry name" value="3-oxoacid_CoA-transf_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012791">
        <property type="entry name" value="3-oxoacid_CoA-transf_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014388">
        <property type="entry name" value="3-oxoacid_CoA-transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004164">
        <property type="entry name" value="CoA_transf_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004163">
        <property type="entry name" value="CoA_transf_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02429">
        <property type="entry name" value="pcaI_scoA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02428">
        <property type="entry name" value="pcaJ_scoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="KETOACID-COENZYME A TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707:SF23">
        <property type="entry name" value="SUCCINYL-COA:3-KETOACID-COENZYME A TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000858">
        <property type="entry name" value="SCOT-t"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01273">
        <property type="entry name" value="COA_TRANSF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01274">
        <property type="entry name" value="COA_TRANSF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="active site" description="5-glutamyl coenzyme A thioester intermediate" evidence="10">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000858-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="530" mass="57403" checksum="6C9B4313F7F2C2CD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHB1</accession>
    <name>A0A452IHB1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IHB1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026856"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030527.1">
        <property type="protein sequence ID" value="ENSGAGP00000026856.1"/>
        <property type="gene ID" value="ENSGAGG00000019553.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052346">
        <property type="entry name" value="O-mannosyl-transferase_TMTC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44227">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44227:SF3">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE TMTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="257"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="346" mass="38574" checksum="88FE874CA4A8F52F" modified="2019-05-08" version="1">MAQSEFNLDHEISWFVLPSYWAKIIVGLISCMCFANSYDGDFVFDDSEAIINNKDLRAETPLGDLWHHDFWGGKLSSNTSHKSYRPLTVLTFRINYLFAGGFHPVGFHVINIILHCAISVLMVDVFSILLGGLQYSSKGRRLNLAPKASLLAASLFAVHPVHTECVAGIVGRADLLCALFFLLSFLGYCKALRENKEGNHFSILWVLASVLLGAIALLCKEQGITVLGLNAVFDALVINKLNIVELIQKVLRKDKSLEVHYNVGKNLADKGNQTAAIKYYREAVRLNPKYVHAMNNLGNILKERNELQEAEGLLSLAVQIQYVKRMASIVASTDHKQYNGILYSKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452II91</accession>
    <name>A0A452II91_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA binding protein fox-1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">RNA-binding protein that regulates alternative splicing events.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452II91"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027575"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031329.1">
        <property type="protein sequence ID" value="ENSGAGP00000027575.1"/>
        <property type="gene ID" value="ENSGAGG00000020025.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12407">
        <property type="entry name" value="RRM_FOX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000004">
        <property type="entry name" value="RNA binding fox-1 homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025670">
        <property type="entry name" value="Fox-1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034237">
        <property type="entry name" value="FOX1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017325">
        <property type="entry name" value="RBFOX1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047131">
        <property type="entry name" value="RBFOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597">
        <property type="entry name" value="ATAXIN 2-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597:SF25">
        <property type="entry name" value="RNA BINDING PROTEIN FOX-1 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12414">
        <property type="entry name" value="Fox-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037932">
        <property type="entry name" value="Ataxin_2_bd_A2BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019242384" description="RNA binding protein fox-1 homolog" evidence="9">
        <location>
            <begin position="19"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="177"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="69"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="150"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037932-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="403" mass="43872" checksum="FD7F8EE700AB320D" modified="2019-05-08" version="1">MLCAHLVVFLYLGWSGESRSPGLCGVFVRFLFQGLAALSLEVQASQQQQQQQQPSHGVCAWITSPKLLGNQDATAPPEAMAQPYPPAQYPPPPQNGIPAEYAPPHPHPTQDYSGQSTVPEHPMTLYTPAQSHAEQPGADGSTQSIAGTQTVSQTDEAAQTESQQLHSSENTDKQQPKRLHVSNIPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGFVTFETSTDADRAREKLNGTIVEGRKIEVNNATARVMTNKKAANPYINGWKLNPVVGAVYSPEFYAVTGFPYPATGTAVAYRGAHLRGRGRAVYNTFRAAPPPPPIPTYGAVVYQDGFYGAEIYGGYAAYRYAQPAAAAAYSDSYGRVYAAADPYHHTIGPAATYSIGTMASLYRGGYSRFTPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJ44</accession>
    <name>A0A452IJ44_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase RNF185</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">RING finger protein 185</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452IJ44"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027909"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031698.1">
        <property type="protein sequence ID" value="ENSGAGP00000027909.1"/>
        <property type="gene ID" value="ENSGAGG00000020268.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000031700.1">
        <property type="protein sequence ID" value="ENSGAGP00000027910.1"/>
        <property type="gene ID" value="ENSGAGG00000020268.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16744">
        <property type="entry name" value="RING-HC_RNF185"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000062">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF185"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045103">
        <property type="entry name" value="RNF5/RNF185-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12313">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="41"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="92"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="194" mass="20829" checksum="5BDE709DD241127C" modified="2019-05-08" version="1">MASKGPTASTSTENSSTGGTSGSSSSNGAGDNTNQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGFGDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPGTPQYVDEQFLSRLFLFVALVIMFWLLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJK8</accession>
    <name>A0A452IJK8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IJK8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028112"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031943.1">
        <property type="protein sequence ID" value="ENSGAGP00000028112.1"/>
        <property type="gene ID" value="ENSGAGG00000020431.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="50" mass="5102" checksum="E8F8BDC0B7968176" modified="2019-05-08" version="1">MAAPLRVSHVACCANRAGGGAVSWGRGGLLAFGTCRSVALYEPEVRGGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IK01</accession>
    <name>A0A452IK01_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IK01"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028209"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032061.1">
        <property type="protein sequence ID" value="ENSGAGP00000028209.1"/>
        <property type="gene ID" value="ENSGAGG00000020505.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019205">
        <property type="term" value="F:nucleobase-containing compound kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22967">
        <property type="entry name" value="DD_AK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047499">
        <property type="entry name" value="DD_AK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007858">
        <property type="entry name" value="Dpy-30_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05186">
        <property type="entry name" value="Dpy-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="616"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="728" mass="83255" checksum="D748ADDF9260DE05" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKN1</accession>
    <name>A0A452IKN1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IKN1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028455"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032323.1">
        <property type="protein sequence ID" value="ENSGAGP00000028455.1"/>
        <property type="gene ID" value="ENSGAGG00000020626.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000032324.1">
        <property type="protein sequence ID" value="ENSGAGP00000028457.1"/>
        <property type="gene ID" value="ENSGAGG00000020626.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000032325.1">
        <property type="protein sequence ID" value="ENSGAGP00000028458.1"/>
        <property type="gene ID" value="ENSGAGG00000020626.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12729">
        <property type="entry name" value="RRM1_hnRNPH_hnRNPH2_hnRNPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12731">
        <property type="entry name" value="RRM2_hnRNPH_hnRNPH2_hnRNPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12734">
        <property type="entry name" value="RRM3_hnRNPH_hnRNPH2_hnRNPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000071">
        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein H isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000075">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein H1 (H)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000031">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein h3 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012996">
        <property type="entry name" value="Znf_CHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08080">
        <property type="entry name" value="zf-RNPHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="78"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="178"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="349"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="525" mass="57233" checksum="43A82D46EB71A327" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILQ0</accession>
    <name>A0A452ILQ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA topoisomerase</fullName>
            <ecNumber evidence="3">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028835"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032760.1">
        <property type="protein sequence ID" value="ENSGAGP00000028835.1"/>
        <property type="gene ID" value="ENSGAGG00000020890.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03488">
        <property type="entry name" value="Topoisomer_IB_N_htopoI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.41:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.11.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.11.10">
        <property type="entry name" value="DNA Topoisomerase I, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.15.10">
        <property type="entry name" value="Topoisomerase I, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.41">
        <property type="entry name" value="Yeast DNA topoisomerase - domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013034">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013030">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001631">
        <property type="entry name" value="TopoI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025834">
        <property type="entry name" value="TopoI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014711">
        <property type="entry name" value="TopoI_cat_a-hlx-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014727">
        <property type="entry name" value="TopoI_cat_a/b-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013500">
        <property type="entry name" value="TopoI_cat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008336">
        <property type="entry name" value="TopoI_DNA-bd_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036202">
        <property type="entry name" value="TopoI_DNA-bd_euk_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013499">
        <property type="entry name" value="TopoI_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018521">
        <property type="entry name" value="TopoIB_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048045">
        <property type="entry name" value="Topoisomer_I_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051062">
        <property type="entry name" value="Topoisomerase_IB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290">
        <property type="entry name" value="DNA TOPOISOMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290:SF1">
        <property type="entry name" value="DNA TOPOISOMERASE I, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14370">
        <property type="entry name" value="Topo_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02919">
        <property type="entry name" value="Topoisom_I_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00416">
        <property type="entry name" value="EUTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00435">
        <property type="entry name" value="TOPEUc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46596">
        <property type="entry name" value="Eukaryotic DNA topoisomerase I, dispensable insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56741">
        <property type="entry name" value="Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00176">
        <property type="entry name" value="TOPO_IB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52038">
        <property type="entry name" value="TOPO_IB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="DNA topoisomerase I eukaryotic-type" evidence="9">
        <location>
            <begin position="251"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="450"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="7">
        <location>
            <position position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="571" mass="66408" checksum="3C8C5435E43699F7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IM38</accession>
    <name>A0A452IM38_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centrosomal protein of 19 kDa</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IM38"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028917"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032848.1">
        <property type="protein sequence ID" value="ENSGAGP00000028917.1"/>
        <property type="gene ID" value="ENSGAGG00000020947.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034454">
        <property type="term" value="P:microtubule anchoring at centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097712">
        <property type="term" value="P:vesicle targeting, trans-Golgi to periciliary membrane compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029412">
        <property type="entry name" value="CEP19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31539:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 19 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31539">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 19K CEP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14933">
        <property type="entry name" value="CEP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="163" mass="19134" checksum="82D231E7BC5F3BB5" modified="2019-05-08" version="1">MTYVAKKCGICFQPPSVILIYEDKNKDRARQRIMPIRNFSKFSDCSRAAEQLKNNPRHKAYLQGVSLQQLRKLYSLLRGHLQGKSLAQSLEQIQQEETIDPEEDLNKLDDKELAKRKSIMDELFEKNRKKKDDPDFTYNVEVEFPQDEQLESCGWDAESDNEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IN71</accession>
    <name>A0A452IN71_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A452IN71"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029309"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033288.1">
        <property type="protein sequence ID" value="ENSGAGP00000029309.1"/>
        <property type="gene ID" value="ENSGAGG00000021204.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18050">
        <property type="entry name" value="DEADc_DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046330">
        <property type="entry name" value="DDX17_ATP-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="86"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="117"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="320"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="86"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="508"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="644" mass="71876" checksum="1E08B8BC7DE1045F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPZ2</accession>
    <name>A0A452IPZ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IPZ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029979"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034046.1">
        <property type="protein sequence ID" value="ENSGAGP00000029979.1"/>
        <property type="gene ID" value="ENSGAGG00000021641.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="12">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="53"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="69" mass="7882" checksum="3685C39C4C9E95ED" modified="2019-05-08" version="1">MAQDLTEKELLKMELDQLKKEVKNERQMVSKTGKELKEYIESMAAEDPLLKGVPEDKNPFKEKGGCIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQX1</accession>
    <name>A0A452IQX1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PKI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IQX1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030251"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034334.1">
        <property type="protein sequence ID" value="ENSGAGP00000030251.1"/>
        <property type="gene ID" value="ENSGAGG00000021805.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004171">
        <property type="entry name" value="cAMP_dep_PKI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15416">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE INHIBITOR/PKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02827">
        <property type="entry name" value="PKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="114"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="121"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="153" mass="16622" checksum="39491FC74D43B8CB" modified="2019-05-08" version="1">MSKPEKRKGNPLKKCLKCQAELGRASFSMQGLCPHCNAKAGEDQKNQKGKRDSYAFGNASEVMEIEPSYSDFISCDRTGRRNAIHDIQGDATSISMRKLAGDIGELSIEGAGNQAAAAAASEKEPEVRLEGQDGTPHHEHHRSGGEEGRRKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IS06</accession>
    <name>A0A452IS06_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamate ionotropic receptor AMPA type subunit 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IS06"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030799"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034928.1">
        <property type="protein sequence ID" value="ENSGAGP00000030799.1"/>
        <property type="gene ID" value="ENSGAGG00000022143.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019585380" description="Glutamate ionotropic receptor AMPA type subunit 4" evidence="1">
        <location>
            <begin position="22"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9214" checksum="02437B73632AD483" modified="2019-05-08" version="1">MRIICRQLVFLFSGFCGLAMGAFPSSVQIGGLFIRNTDQEYTAFRLAIFLHNTSPNASEAPFNLVPHVDNIETANSFAVTNACK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IS07</accession>
    <name>A0A452IS07_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PA-PLA1 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000030904"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035045.1">
        <property type="protein sequence ID" value="ENSGAGP00000030904.1"/>
        <property type="gene ID" value="ENSGAGG00000022179.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004177">
        <property type="entry name" value="DDHD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058055">
        <property type="entry name" value="PA-PLA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509">
        <property type="entry name" value="PA-PL1 PHOSPHOLIPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509:SF32">
        <property type="entry name" value="PHOSPHOLIPASE DDHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02862">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01127">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51043">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDHD" evidence="3">
        <location>
            <begin position="512"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="606"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="667"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="614"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="679"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="791" mass="88570" checksum="3CB1E8159811B865" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUN7</accession>
    <name>A0A452IUN7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Structural maintenance of chromosomes protein 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination and mediates sumoylation of shelterin complex (telosome) components.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Forms a heterodimer with smc5. Component of the SMC5-SMC6 complex which consists at least of smc5, smc6, nsmce2, nsmce1 and nsmce4a.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IUN7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031595"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035833.1">
        <property type="protein sequence ID" value="ENSGAGP00000031595.1"/>
        <property type="gene ID" value="ENSGAGG00000022691.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000035837.1">
        <property type="protein sequence ID" value="ENSGAGP00000031599.1"/>
        <property type="gene ID" value="ENSGAGG00000022691.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003232">
        <property type="entry name" value="Structural maintenance of chromosomes 6, gene 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000959">
        <property type="entry name" value="structural maintenance of chromosomes protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306:SF6">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="18">
        <location>
            <begin position="58"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="751"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="312"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="829"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="753"/>
            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1096" mass="127491" checksum="E2F49905BCDA41D0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IVJ4</accession>
    <name>A0A452IVJ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GKAP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IVJ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032142"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036441.1">
        <property type="protein sequence ID" value="ENSGAGP00000032142.1"/>
        <property type="gene ID" value="ENSGAGG00000023021.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026109">
        <property type="entry name" value="GKAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14899">
        <property type="entry name" value="G KINASE ANCHORING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14899:SF0">
        <property type="entry name" value="G KINASE-ANCHORING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02083">
        <property type="entry name" value="GKINASEAP1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="144"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="205"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="331" mass="37402" checksum="8CA2B91FDE2A4F15" modified="2019-05-08" version="1">MASAVISSVPTTASRFALLQVDSGSGSDSETGKGRSGRGAGKSQASGGKSTTNEKKKEKRRKKKEQQQSEANELRNLAFKKIPQKSSHGGCLSQHEQTLHSPVQKDSQEENWQEWRQRDEQLTSEMFEADLEKALLLSKLEYEEHKKDSENASPPSKSANKKEKRKNQQGKDKPLTVSLKDFQSDINIDPLAKKHEEAVLKDGKTERLKLELEKKDTEIQQLKSIITQWEVKYKEVKARNAQLLKMLQEGEMKDKAEILLQVDESQSIKNELTLQVTTLHAALEQERSKVKLLQAELTKYQVFIFVLSLEINSPMSSIILIVCGNIDTRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IW45</accession>
    <name>A0A452IW45_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IW45"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036420.1">
        <property type="protein sequence ID" value="ENSGAGP00000032123.1"/>
        <property type="gene ID" value="ENSGAGG00000023014.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000140">
        <property type="entry name" value="CUGBP Elav-like family member 4 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="233"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="297" mass="31286" checksum="2F9EB1F5A59C7BA6" modified="2019-05-08" version="1">LPPLAGCAFVKYSSHAEAQAAINALHGSQTMPGASSSLVVKFADTDKERTMRRMQQMAGQMGMFNPMAIQFGAYGAYAQALMQQQAAIMASVAQGGYLNPMAAFAAAQMQQMAALNMNGLAAAPMTPTSGGSTPPGITAPAVPSIPSPIGVNGFTGLPPQANGQPTAEAVFANGIHPYPAQSPTAADPLQQAYAGVQQYAAAYPAAYGQISQAFPQPPPMIPQQQREGPEGCNLFIYHLPQEFGDAELMQMFLPFGFVSFDNPASAQAAIQAMNGFQIGMKRLKVQLKRPKDANRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J324</accession>
    <name>A0A452J324_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J324"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034811"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039406.1">
        <property type="protein sequence ID" value="ENSGAGP00000034811.1"/>
        <property type="gene ID" value="ENSGAGG00000024728.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="326"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="52"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="454" mass="49537" checksum="DFB5AC54301EAB59" modified="2019-05-08" version="1">MSQAVDLPTAELPVQYKGWSGMWTFAVYDNYPIPMLLGEDLANQVRRAKRVGMVTRSQTRQASRPIPVPEPSTEAPSVLPETQTEVVDPDSMPTTETATASPVPGPELEQQPAPASATTSSNSTPEGASEPQLAEAQDSHTQKAQPEPEIPSGAPAESGSPATETTPSPTSLPEGPSPSPQSEEELVTPASREQFQAEQEADDSLQKAWAAARSTPPPLSSSNRSRFVIDQGLLYKEILSGGHREEWQPQKQLVVPTKYRGKLLSLAHDHPSGHAGVNRTKDRLGKSFHWEGMGKDVAKYVRSCEVCQRVGKPQDQVKAPLQPLPIIEVPFQRVAVDILGPFPKKTPRGKQYVLTLVDFATRWPEAVALGNTRANTVCLALTDIFARVGWPSDILTDSGSNFLAGTMEKLWETHGVNHLVATPYHHQTNGLVERFNGTLGAMIHKFVNEYSNNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J3Z0</accession>
    <name>A0A452J3Z0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J3Z0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035146"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039795.1">
        <property type="protein sequence ID" value="ENSGAGP00000035146.1"/>
        <property type="gene ID" value="ENSGAGG00000024982.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001851">
        <property type="term" value="F:complement component C3b binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006956">
        <property type="term" value="P:complement activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000026">
        <property type="entry name" value="Complement inhibitory factor H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000060">
        <property type="entry name" value="Complement inhibitory factor H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051503">
        <property type="entry name" value="ComplSys_Reg/VirEntry_Med"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785:SF7">
        <property type="entry name" value="COMPLEMENT FACTOR H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785">
        <property type="entry name" value="COMPLEMENT FACTOR H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="1 4" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019213491" description="Sushi domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="24"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="26"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="219" mass="24866" checksum="04B95313AEDCA9A7" modified="2019-05-08" version="1">MTLLRGIIIQILWAFYAKGQESAGKCGHPPAIDNGDVLVFLQEEYDSGSRVEYKCQSFYRMKGSAFVSCESGQWTNPPVCLEPCTITSEVMEKNNIRLKWSSKTRLYSESGDFVEFDCKNGYVKDPTSSAFRVQCVEGKLAYPKCKREELCITFPEDMEKNNIGLKWSSRTRLNLRSGDFVEFDCKNGYVRDPTSSAFRVQCVEGKLAYPKCKRIGTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4C2</accession>
    <name>A0A452J4C2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">F-box/LRR-repeat protein 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">F-box and leucine-rich repeat protein 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. During mitosis, it mediates the ubiquitination and subsequent proteasomal degradation of AURKA, causing mitotic arrest. It also regulates mitochondrial function by mediating the ubiquitination and proteasomal degradation of the apoptosis inhibitor BIRC5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXL7) composed of CUL1, SKP1, RBX1 and FBXL7. Interacts with AURKA; interaction takes place during mitosis but not in interphase. Interacts with BIRC5; this interaction allows BIRC5 to be polyubiquitinated by the SCF(FBXL7) E3 ubiquitin-protein ligase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the FBXL7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J4C2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035288"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039956.1">
        <property type="protein sequence ID" value="ENSGAGP00000035288.1"/>
        <property type="gene ID" value="ENSGAGG00000025082.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22120">
        <property type="entry name" value="F-box_FBXL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000122">
        <property type="entry name" value="F-box/LRR-repeat protein 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000126">
        <property type="entry name" value="F-box/LRR-repeat protein 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000018">
        <property type="entry name" value="F-box/LRR-repeat protein 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF50">
        <property type="entry name" value="F-BOX_LRR-REPEAT PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="16">
        <location>
            <begin position="102"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="482" mass="54277" checksum="B76D6AB460A6930A" modified="2019-05-08" version="1">MRLPVKQTKELALGISQEEVIVAGVVTERQKAKDSDLSMRTLSTPSPALICPQNSHGFQNGRGSSSSSSSVTGETVAMVHSPPPTRLTHPLIRLASRQQKEQANIDRLPDHSMIQIFSFLPTNQLCRCARVCRRWYNLAWDPRLWRTIRLTGETINVDRALKVLTRRLCQDTPNVCLMLETVIVSGCRRLTDRGLYTIAQCCPELRRLEVSGCYNISNEAVFDVVSLCPNLEHLDVSGCSKVTCISLTREASIKLSPLHGKQISIRYLDMTDCFVLEDEGLHTIAAHCTQLTHLYLRRCVRITDEGLRYLMIYCTSIKELSVSDCRFVSDFGMREIAKLESRLRYLSIAHCGRITDVGIRYIAKYCSKLRYLNARGCEGITDHGVEYLAKNCTKLKSLDIGKCPLVSDTGLEFLALNCFNLKRLSLKSCESITGQGLQIVAANCFDLQMLNVQDCEVSVDALRFVKRHCKRCIIEHTNPAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5U8</accession>
    <name>A0A452J5U8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Syndecan</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Cell surface proteoglycan.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Membrane</location>
            <topology evidence="1 10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the syndecan proteoglycan family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J5U8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035731"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040458.1">
        <property type="protein sequence ID" value="ENSGAGP00000035731.1"/>
        <property type="gene ID" value="ENSGAGG00000025367.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003585">
        <property type="entry name" value="Neurexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001050">
        <property type="entry name" value="Syndecan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027789">
        <property type="entry name" value="Syndecan/Neurexin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030479">
        <property type="entry name" value="Syndecan_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10915">
        <property type="entry name" value="SYNDECAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10915:SF6">
        <property type="entry name" value="SYNDECAN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01034">
        <property type="entry name" value="Syndecan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00294">
        <property type="entry name" value="4.1m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00964">
        <property type="entry name" value="SYNDECAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019478340" description="Syndecan" evidence="13">
        <location>
            <begin position="20"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Neurexin/syndecan/glycophorin C" evidence="14">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="41"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="67"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="204" mass="22298" checksum="36E7C823D6EE1058" modified="2019-05-08" version="1">MHGGFCLWTARLSLKLSLAGRAELTSDKDLYLDNSSVEEASGVYPIDDDDYSSGSGSGAEDDEDNTVIATSRTLPKIPSTSDAPKAETTTLKTQTKIPAQTKSPEESDDKEKIQKTNSNTNKNKNDPGDDADVFTEKHSENLFQRTEVLAAVIAGGVIGFLFAIFLILLLVYRMRKKDEGSYDLGERKPSSAAYQKAPTKEFYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J725</accession>
    <name>A0A452J725_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the COLEC10/COLEC11 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000036304"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041102.1">
        <property type="protein sequence ID" value="ENSGAGP00000036304.1"/>
        <property type="gene ID" value="ENSGAGG00000025728.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03591">
        <property type="entry name" value="CLECT_collectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000005">
        <property type="entry name" value="collectin-11 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051663">
        <property type="entry name" value="CLec_Tetranectin-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033990">
        <property type="entry name" value="Collectin_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22799:SF1">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 11 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22799">
        <property type="entry name" value="TETRANECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-0465">Mannose-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="C-type lectin" evidence="11">
        <location>
            <begin position="132"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="253" mass="28017" checksum="2931E2575056EB47" modified="2019-05-08" version="1">GFCYSRLSLRRSGFXEEKFLLVVLVSGFCLVGDDGEKGDQGEVGKHGKVGPKGPKGTCGSFDSIDLGILIQIKQNNIGIKGLTGVPGGKGKAGTVCECGRYRKVVGQLDINVARLKTAMKFVKNVIAGIRETDEKFYYIVQEEKSYKEALTHCRIRGGMLAMPKDEAANALIADYISKSGLFRVFIGVNDMEKEGQFMYADNTPLQNYSNWKEGEPKNTSVHEDCVEMLSTGEWNDSECHLAIYFVCEFKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7D2</accession>
    <name>A0A452J7D2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Outer dense fiber of sperm tails 3 like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J7D2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036407"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041234.1">
        <property type="protein sequence ID" value="ENSGAGP00000036407.1"/>
        <property type="gene ID" value="ENSGAGG00000025817.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF3">
        <property type="entry name" value="OUTER DENSE FIBER PROTEIN 3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="29926" checksum="720EFBE3C33B599A" modified="2019-05-08" version="1">MSKSIGPNRAGNYGASTCRTSATAPGGVKQYPPISAKIKGPGAGKYGRQPCTGIRNHDFTMFAEPAYTMHCRGVYKRTHINFTHRKSTGDIKICTKLTNTCMQIPLSSVLTILRDLTPAPNEYHTEKVHPQGEPYAPAYSLADRTRYCENDPNPAPNAYTLPGTLGPRLPIKPSAPCYSMASKMDMQRNAEMLARVPGPASFVVPEPNVYLNRQPAYSMWRKYKFIRDSTPGPADYKTSRVTIIKPRAPDFSLGIRHSEYLTPLVIDVME</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7H7</accession>
    <name>A0A452J7H7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-processing factor 39</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">PRP39 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PRP39 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J7H7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036463"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041296.1">
        <property type="protein sequence ID" value="ENSGAGP00000036463.1"/>
        <property type="gene ID" value="ENSGAGG00000025861.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000063">
        <property type="entry name" value="Pre-mRNA processing factor 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000091">
        <property type="entry name" value="Pre-mRNA-processing factor 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204">
        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF24">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 39-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="644"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="759" mass="86126" checksum="5141A48B420D24DB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GF54</accession>
    <name>A0A452GF54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATPase H+ transporting V1 subunit E1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GF54"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000182"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000211.1">
        <property type="protein sequence ID" value="ENSGAGP00000000182.1"/>
        <property type="gene ID" value="ENSGAGG00000000159.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2320.30:FF:000001">
        <property type="entry name" value="V-type proton atpase subunit e 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="25898" checksum="6F0F0E780913F14F" modified="2019-05-08" version="1">MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKIQMSNLMNQARLKVLKARDDLISDLLAEAKQRLAKVVKDGARYQTLLDGLVLQGFYQLLEPRLNVRCRKQDLSMVKAAVQKSIPIYKAATKKDIDVHIDQQTFLPEDIAGGVEIYNSDGKIKVSNTLESRLDLIAQQMMPEIRGALFGANANRKFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGV2</accession>
    <name>A0A452GGV2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Folate receptor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the folate receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GGV2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000795"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000896.1">
        <property type="protein sequence ID" value="ENSGAGP00000000795.1"/>
        <property type="gene ID" value="ENSGAGG00000000517.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004269">
        <property type="entry name" value="Folate_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018143">
        <property type="entry name" value="Folate_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10517">
        <property type="entry name" value="FOLATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10517:SF14">
        <property type="entry name" value="FOLATE RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03024">
        <property type="entry name" value="Folate_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018999031" description="Folate receptor-like domain-containing protein" evidence="4">
        <location>
            <begin position="28"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Folate receptor-like" evidence="5">
        <location>
            <begin position="35"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="254" mass="28733" checksum="BC182572CBA7D229" modified="2019-05-08" version="1">QSQRLTSREMAVWWAVLGLLAACVASAAKESVLNVCMDAKHHKTKPGPEGALHGQCALWKDNACCTAETSTGAHQDQSYLYNFNWNHCGVMPEMCKRHFIQDTCLYECSPNLGPWIDQADTSWRRERILNVPLCKEDCELWWEACKDAVTCKENWHKGWNWTSGTNQCPHSSTCQLFKYIFPRPADLCEKIWSNSYKYTTEHWGSGRCIQMWFDPANGNPNVAVAKYYAQNGRDASPAPQAVLLLLPAALLSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGW2</accession>
    <name>A0A452GGW2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GGW2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000806"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000908.1">
        <property type="protein sequence ID" value="ENSGAGP00000000806.1"/>
        <property type="gene ID" value="ENSGAGG00000000678.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475:SF135">
        <property type="entry name" value="EOSINOPHIL PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475">
        <property type="entry name" value="OXIDASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="93" mass="10550" checksum="F89A8204E65A7603" modified="2019-05-08" version="1">KHSYFGTSNHGYARWLPAEYEDGVSLPKGFTEGKLYNGFPLPLVRKVSNEIIHTANENVTQDQQRSVIFVHWGQWVDHDLDLAPAPVTKITNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GH89</accession>
    <name>A0A452GH89_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GH89"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000974"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001101.1">
        <property type="protein sequence ID" value="ENSGAGP00000000974.1"/>
        <property type="gene ID" value="ENSGAGG00000000789.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000207">
        <property type="entry name" value="paired mesoderm homeobox protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF303">
        <property type="entry name" value="PAIRED MESODERM HOMEOBOX PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="90"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="92"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="148"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="280" mass="30558" checksum="0C5C34A150E19388" modified="2019-05-08" version="1">MYKMDYSYLNSYDSCMAAMEASAYADFNSCSQANTFQYNPIRTGFGSNPGCPPLSTANCTLGALRDHQPSPYSTVPYKFFSDPSGINEKRKQRRIRTTFTSSQLKELERVFAETHYPDIYTREELALKIDLTEARVQVWFQNRRAKFRKQERAANAKGVNGSSSANKKSDPRSSSEDDESKESNCSPTPDSTASLPPTGSLNSPGSSLSPSPGTGQGLGPSHATQSLKAPIWPGVSTSNGATNTAELLKAWQPAEAMPGPFSGVLSSFHRKPNVLKTNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHM2</accession>
    <name>A0A452GHM2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 12">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 12">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 12">Cell membrane</location>
            <topology evidence="3 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the connexin family. Alpha-type (group II) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GHM2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001109"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001244.1">
        <property type="protein sequence ID" value="ENSGAGP00000001109.1"/>
        <property type="gene ID" value="ENSGAGG00000000901.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034634">
        <property type="entry name" value="Connexin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF54">
        <property type="entry name" value="GAP JUNCTION ALPHA-4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118220">
        <property type="entry name" value="Connexin43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0303">Gap junction</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="15">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="16">
        <location>
            <begin position="165"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="295"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="334" mass="37795" checksum="9EB8237A1954BA87" modified="2019-05-08" version="1">MGDWGFLEKLLDQVQEHSTVIGKIWLTVLFIFRILILGLAGESVWGDEQSDFLCNTKQPGCVNVCYDKAFPISHIRYWVLQFLFVSTPTLIYLGHVIYLSGREEKLKQRESELRAVQIKDLKVEQALSAVQKKISKICVAEDGRIKIRGSLMWTYVVSVICKSIFEAGFLLGQWYLYGFFMPPVFVCERAPCPHKVDCFVSRPTEKTIFIIFMMVVGLISLGLNLLELFHLCCKNLLSSMREASSSPSPAVDIDSFPDSKQCHYIPLSESHSPPYLAYNKLSSEQNWANFNTEENLALRSGNKPSPESYAPGAQPLQERQTSRPGSSASKKQYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJA5</accession>
    <name>A0A452GJA5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small integral membrane protein 15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMIM15 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GJA5"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002001.1">
        <property type="protein sequence ID" value="ENSGAGP00000001759.1"/>
        <property type="gene ID" value="ENSGAGG00000001411.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027877">
        <property type="entry name" value="Smim15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28644">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28644:SF1">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15086">
        <property type="entry name" value="UPF0542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="74" mass="8669" checksum="5FA69D18A508498B" modified="2019-05-08" version="1">MFDIKAWAEYVVEWAAKDPYGFLTTVILALTPLFLASAVLSWKLAKMIEAREREQKKKQKRQENIAKAKRTKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJX1</accession>
    <name>A0A452GJX1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GJX1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001899"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002158.1">
        <property type="protein sequence ID" value="ENSGAGP00000001899.1"/>
        <property type="gene ID" value="ENSGAGG00000001525.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005307">
        <property type="term" value="F:choline:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008292">
        <property type="term" value="P:acetylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052244">
        <property type="entry name" value="Choline_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45897:SF2">
        <property type="entry name" value="HIGH AFFINITY CHOLINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45897">
        <property type="entry name" value="HIGH-AFFINITY CHOLINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="337" mass="37227" checksum="CF879F22E2FC8709" modified="2019-05-08" version="1">MLGGIPWQAYFQRVLSSSSATYAQVLSFLAAFGCLVMALPAILIGAIGASTDWNKTMYGLPDPTERQEADMILPIVLQYLCPVYISFFGLGAVSAAVMSSADSSILSASSMFARNIYQLSFRQNASDREIIWVMRITIFVFGAAATAMALLAKSVYGLWYLSSDLIYIIIFPQLLCVLFIKGTNTYGAIAGYLFGLLLRITGGEPYLYLQPLIFYPGYYADKNHIYVQRFPFKTLAMLTSFFTNIAVSYLAKYLFESGSLPPKLDFLDAVVARYSQENMDKATLVKSDNIILNELAPVNPRCSLTLNSTFTNKEAFSDVDSSPELSNAEDNLVTNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMJ7</accession>
    <name>A0A452GMJ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Inositol 1,4,5-trisphosphate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 19">Endoplasmic reticulum membrane</location>
            <topology evidence="1 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="19">The ITPR1 structure has a large solenoid CY assembly built around the central helical bundle made of the C-terminal domains from four ITPR1 subunits. The solenoid scaffold includes domains responsible for binding of ligands and regulatory proteins and is connected via an allosteric nexus at the cytosolic-membrane interface to the transmembrane channel assembly. Six transmembrane helices from each subunit form the central ion-conduction pore.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the InsP3 receptor family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000003047"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003495.1">
        <property type="protein sequence ID" value="ENSGAGP00000003047.1"/>
        <property type="gene ID" value="ENSGAGG00000002436.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005220">
        <property type="term" value="F:inositol 1,4,5-trisphosphate-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23287">
        <property type="entry name" value="beta-trefoil_MIR_ITPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000002">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.30:FF:000001">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor, type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.30">
        <property type="entry name" value="IP3 receptor type 1 binding core, RIH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000493">
        <property type="entry name" value="InsP3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035910">
        <property type="entry name" value="RyR/IP3R_RIH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF2">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00779">
        <property type="entry name" value="INSP3RECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100909">
        <property type="entry name" value="IP3 receptor type 1 binding core, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 19" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="5 19" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16 19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 19" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 19" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="2273"/>
            <end position="2295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="2307"/>
            <end position="2325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="2331"/>
            <end position="2357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="2393"/>
            <end position="2418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="2439"/>
            <end position="2461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="2573"/>
            <end position="2596"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="21">
        <location>
            <begin position="112"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="21">
        <location>
            <begin position="231"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="21">
        <location>
            <begin position="379"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1006"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1137"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1753"/>
            <end position="1786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1879"/>
            <end position="1900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1928"/>
            <end position="1952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2723"/>
            <end position="2752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1006"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1149"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="1769"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1881"/>
            <end position="1895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1928"/>
            <end position="1939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="2740"/>
            <end position="2752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU368044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2752" mass="313599" checksum="25392BCF56C3A1EF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMK9</accession>
    <name>A0A452GMK9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KAP NTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GMK9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003067"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003523.1">
        <property type="protein sequence ID" value="ENSGAGP00000003067.1"/>
        <property type="gene ID" value="ENSGAGG00000002459.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011646">
        <property type="entry name" value="KAP_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052754">
        <property type="entry name" value="NTPase_KAP_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22674:SF4">
        <property type="entry name" value="NTPASE KAP FAMILY P-LOOP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22674">
        <property type="entry name" value="NTPASE, KAP FAMILY P-LOOP DOMAIN-CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07693">
        <property type="entry name" value="KAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="KAP NTPase" evidence="2">
        <location>
            <begin position="49"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="589" mass="67206" checksum="A68547F03F13F517" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMP3</accession>
    <name>A0A452GMP3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Keratin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the avian keratin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMP3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003098"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003563.1">
        <property type="protein sequence ID" value="ENSGAGP00000003098.1"/>
        <property type="gene ID" value="ENSGAGG00000002493.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003461">
        <property type="entry name" value="Keratin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31203">
        <property type="entry name" value="BETA-KERATIN-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31203:SF1">
        <property type="entry name" value="BETA-KERATIN-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02422">
        <property type="entry name" value="Keratin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0416">Keratin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="17399" checksum="C45BD561E6DC21E4" modified="2019-05-08" version="1">MIPNILFPLGPSFDSSPCCAFQVYLHPRKITLSSLCYPECGVARPWPVTGACNQPCVRQCPDSELVIRPSLVVVTHPGPILSNFSQHSAVGAVGAPAIRPGFGCSFGWVGYGGRYGGWYGLGGYGGYGSPYGYWGHGGYLGDYGYGGICGSGVSCHRYLSGSCGPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNI5</accession>
    <name>A0A452GNI5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GNI5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003437"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003962.1">
        <property type="protein sequence ID" value="ENSGAGP00000003437.1"/>
        <property type="gene ID" value="ENSGAGG00000002782.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="20"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="90" mass="10163" checksum="123BDDDC7A7AE920" modified="2019-05-08" version="1">MELDKDRCIKMNDGKKIPALGFGTYCSDVLKSKCEEDIKVAIEVGFRHIDGAFVYGTEEEVGRAVHEKIADGTVKREDIFYTGKVKFLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNT4</accession>
    <name>A0A452GNT4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the smoothelin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GNT4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003548"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004091.1">
        <property type="protein sequence ID" value="ENSGAGP00000003548.1"/>
        <property type="gene ID" value="ENSGAGG00000002861.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd21258">
        <property type="entry name" value="CH_SMTNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000009">
        <property type="entry name" value="smoothelin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022189">
        <property type="entry name" value="SMTN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF52">
        <property type="entry name" value="SMOOTHELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12510">
        <property type="entry name" value="Smoothelin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="831"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="202"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="650"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="450"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="532"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="557"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="601"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="650"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="680"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="706"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="739"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="949" mass="104209" checksum="DA6152DA902F234D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPL0</accession>
    <name>A0A452GPL0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c oxidase subunit 5B, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GPL0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003735"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004321.1">
        <property type="protein sequence ID" value="ENSGAGP00000003735.1"/>
        <property type="gene ID" value="ENSGAGG00000003034.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.11.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002124">
        <property type="entry name" value="Cyt_c_oxidase_su5b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036972">
        <property type="entry name" value="Cyt_c_oxidase_su5b_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122:SF11">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01215">
        <property type="entry name" value="COX5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51359">
        <property type="entry name" value="COX5B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602124-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12083" checksum="C4013D0F1A054D4E" modified="2019-05-08" version="1">MASRLLLRVLQRALPGGGRAAAPARAMSAGGIPSDEEQATGLERKTLEAMKKGQDPYSILKPKGYAGTREDPHIVPSVNKKRLVGCICMYQNNFVYVLLGHILSNVIKHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GR51</accession>
    <name>A0A452GR51_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSGAGT00000004901.1">
        <property type="protein sequence ID" value="ENSGAGP00000004184.1"/>
        <property type="gene ID" value="ENSGAGG00000003459.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="59" mass="6518" checksum="EDB2C1CA01035054" modified="2019-05-08" version="1">MWMVLSKIKVPCHSNGALRFGVIAKGITVLHAVPLGGNHTVIXSRNSEPNLLDYLARFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSJ9</accession>
    <name>A0A452GSJ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Large ribosomal subunit protein uL2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">60S ribosomal protein L8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GSJ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004968"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005805.1">
        <property type="protein sequence ID" value="ENSGAGP00000004968.1"/>
        <property type="gene ID" value="ENSGAGG00000004064.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000006">
        <property type="entry name" value="60S ribosomal protein L8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000581">
        <property type="entry name" value="Ribosomal protein L8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_A">
        <property type="entry name" value="Ribosomal_uL2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_uL2_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007180">
        <property type="entry name" value="PRK09612.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="13">
        <location>
            <begin position="11"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="12">
        <location>
            <begin position="96"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="257" mass="28023" checksum="662B6AF7DDADBDD0" modified="2019-05-08" version="1">MGRVIRGQRKGAGSVFRAHVKHRKGPAKLRAIDFAERHGYIKGIVKDIIHDPGRGAPLAKVAFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGIVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQEKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSS8</accession>
    <name>A0A452GSS8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GSS8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005048"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005889.1">
        <property type="protein sequence ID" value="ENSGAGP00000005048.1"/>
        <property type="gene ID" value="ENSGAGG00000004079.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003131">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000638">
        <property type="entry name" value="Zinc finger protein 184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000128">
        <property type="entry name" value="zinc finger protein 268 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000829">
        <property type="entry name" value="zinc finger protein 510"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000384">
        <property type="entry name" value="Zinc finger protein 550"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001437">
        <property type="entry name" value="Zinc finger protein 594"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF377">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="7"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="276"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="332"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="360"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="416"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="472"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="504" mass="57699" checksum="E6FE9A5B0D43B844" modified="2019-05-08" version="1">MLSPGPVTFEEVAVYFTREEWALLDPAQRALYRHVMQENYKNVTSLGKDSHPLTVGPRFPGLRGKRDPERQLHDKDLVRSLGSGWSSAGVLSSGQILFLKIHTGVRPYGCCECGKTFAERSTFIRHQRIHIGVRPYGCCECGKTFTERSTLIRHQRIHTGEKPYGCCECGKTFAQRSQLLSHQRIHTGVRPYGCCECGKTFTEHSRLIRHQRSHTGEKPHGCCECGKSFAQRSHLIRHQKIHTGEKPYGCYECEKTFTERSHLIHHQRSHTGEKPHECCECGKTFTDHSCLIRHQRSHTGEKPYGCCECGKTFARRSHLIRHQRIHTGEKPYGCCECGKTFARRSHLIRHQRSHTGEKPYECCECGKTFARSSHLITHQRSHTGEKPYECRECGKTFTQISGLISHQRSHTGEKPYGCRECGKTFAHFSNLITHQRIHTGEKPYKCRECGKTFAQHPHLIAHQRIHTGEKPYECHECGKTFTGRSGLTTHQRNHTGEKPYGCCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTN6</accession>
    <name>A0A452GTN6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="6">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTN6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005406"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006294.1">
        <property type="protein sequence ID" value="ENSGAGP00000005406.1"/>
        <property type="gene ID" value="ENSGAGG00000004388.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF142">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="7">
        <location>
            <begin position="28"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="6">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="155" mass="17149" checksum="505C3F8008D9CBAA" modified="2019-05-08" version="1">MMDQPRCSLPDIIGTSELMRRRKRYAHSGSVWPVKELTWRVRSYPQASGLPRDHVRTLLFYALQAWANASALTFREAPGAGADLLVEFSRSFHEDGYPFDGPGSTLAHAFFPGEHPISGDTHFDDEETWIYDPQAKQQGQCVKGGVGGGAVGQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUR6</accession>
    <name>A0A452GUR6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GUR6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005841"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006816.1">
        <property type="protein sequence ID" value="ENSGAGP00000005841.1"/>
        <property type="gene ID" value="ENSGAGG00000004521.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000004">
        <property type="entry name" value="Unconventional myosin ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF353">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 8" id="KW-0518">Myosin</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin motor" evidence="9">
        <location>
            <begin position="27"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="10">
        <location>
            <begin position="835"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="8">
        <location>
            <begin position="575"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="120"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1012" mass="116472" checksum="B4D5D86EAC19DD88" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVC3</accession>
    <name>A0A452GVC3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the INSM1 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000006073"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007075.1">
        <property type="protein sequence ID" value="ENSGAGP00000006073.1"/>
        <property type="gene ID" value="ENSGAGG00000004927.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010564">
        <property type="term" value="P:regulation of cell cycle process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001458">
        <property type="entry name" value="INSM transcriptional repressor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000488">
        <property type="entry name" value="Insulinoma-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042972">
        <property type="entry name" value="INSM1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15065">
        <property type="entry name" value="INSULINOMA-ASSOCIATED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15065:SF5">
        <property type="entry name" value="INSULINOMA-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="378"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="406"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="41"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="120"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="273"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="347"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="282"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="447" mass="48136" checksum="FBF81BF7B75BE55E" modified="2019-05-08" version="1">MPRGFLVKRNRRVTPISYRVRCGQEGEPELLLFAGRQQLCSPPLPSAGPDPAAPTAPREPPLPPHKPVQFGTPEAVCQALYSPTRPVSKEHEKKSLERSFNLGSPVSAESFPTPALKLWASSSSSSPAETSGPHATLFSGQTSAGTRSLSALLPASSARPQPKRPPAGSEPGKHKPPAAKKAKAIRKLNFEDEVTTSPVLGLKIKEGPVEPPRARGAGGPGPRPLGEFICQLCKEEYADPFALAQHKCSRIVRVEYRCPECDKVFSCPANLASHRRWHKPRPAPSAPPPPAKAPEEQLQPKEAGGSSSSERDTPSPGSGGGXSEEGLYECPTCARRFRRQAYLRKHLLGHEPGPGPERTSPEESPSPSPGAPTASECHLCPVCGETFPSKGGQERHLRLLHSAQVFPCKYCPATFYSSPGLTRHINKCHPSENRQVILLQLPVRPAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVR0</accession>
    <name>A0A452GVR0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GVR0"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007032.1">
        <property type="protein sequence ID" value="ENSGAGP00000006038.1"/>
        <property type="gene ID" value="ENSGAGG00000004902.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="157" mass="16842" checksum="F54C74FA3F5FEE0E" modified="2025-10-08" version="2">MPTPLSPNLPPHCYPSPGIPAHTPLPTPLSPDLPPRCYPSPGIPAHTPLPTPLSPDLPPRCYPSPGIPAHTPLPTPLSPDLPPRCYPSPGIPAHTPLPTPLSPDLPPRCYPSPGIPAQARTQQCHPQLPRQPGSMGLRVMCWLNPYRVQPRPKCLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXD3</accession>
    <name>A0A452GXD3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GXD3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006801"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007895.1">
        <property type="protein sequence ID" value="ENSGAGP00000006801.1"/>
        <property type="gene ID" value="ENSGAGG00000005489.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF118">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="424"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="443"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="555" mass="64203" checksum="24E5370B6A627D8A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXR3</accession>
    <name>A0A452GXR3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GXR3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006944"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008047.1">
        <property type="protein sequence ID" value="ENSGAGP00000006944.1"/>
        <property type="gene ID" value="ENSGAGG00000005597.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF142">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="19"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="85" mass="10004" checksum="FED69D6896C08DCE" modified="2019-05-08" version="1">QPAAAALRLPSSEGNTGQLTFEEVAVWFSREEWEMLADWQKELYRAVMLENYENLFLLGNKPGHRFMFRRVQNPFDKHLKYPGMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZN8</accession>
    <name>A0A452GZN8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GZN8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008795.1">
        <property type="protein sequence ID" value="ENSGAGP00000007619.1"/>
        <property type="gene ID" value="ENSGAGG00000006095.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001230">
        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="98" mass="10663" checksum="82220026263EE71A" modified="2019-05-08" version="1">DIVLTQTPESLAVSPGDRVTINCKASSSVSSYMAWYQQKSGQAPKLLIYSASTRPSGIPDRFSGSGSGTDFTLTITSFQADDAGSYYCQQYYDWPLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0U4</accession>
    <name>A0A452H0U4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nuclear protein localization protein 4 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="5">Protein degradation; proteasomal ubiquitin-dependent pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NPL4 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000008120"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009353.1">
        <property type="protein sequence ID" value="ENSGAGP00000008120.1"/>
        <property type="gene ID" value="ENSGAGG00000005771.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08061">
        <property type="entry name" value="MPN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000084">
        <property type="entry name" value="nuclear protein localization protein 4 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000012">
        <property type="entry name" value="nuclear protein localization protein 4 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016563">
        <property type="entry name" value="Npl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007717">
        <property type="entry name" value="NPL4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024682">
        <property type="entry name" value="Npl4_Ub-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007716">
        <property type="entry name" value="NPL4_Zn-bd_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710:SF0">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05021">
        <property type="entry name" value="NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11543">
        <property type="entry name" value="UN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05020">
        <property type="entry name" value="zf-NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010052">
        <property type="entry name" value="Polyub_prc_Npl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MPN" evidence="8">
        <location>
            <begin position="228"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="572"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00074519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="628" mass="70801" checksum="F9AB394D64CDF988" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H175</accession>
    <name>A0A452H175_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">E3 ubiquitin-protein ligase CCNB1IP1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000008346"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009608.1">
        <property type="protein sequence ID" value="ENSGAGP00000008346.1"/>
        <property type="gene ID" value="ENSGAGG00000006633.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000795">
        <property type="term" value="C:synaptonemal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042448">
        <property type="entry name" value="CCNB1IP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14305">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CCNB1IP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14305:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CCNB1IP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="100"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27655" checksum="81EFDA9E902EAEA7" modified="2019-05-08" version="1">MQVNLTLRGVPICFFYRLDSXPPSHPRSYCPLFQFLSSMSLERLSFELWSKKPVTPVIISTWQVHQEHLYQEYTYSKAEGHLKQIEKVYTQQLQSKDMELTSMKSEVSSLKKMLEEYKKKFSELSEKLMERNRQYQKLQGLYDSLRLRNIAMANQEAAHEPPMMPQSAVFGFPLGNAAKFPGHATPVRGRCGEGDFHFKPFFATSPPADESSNSFFTFASPSNELEPHPGASRAYKMKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1M4</accession>
    <name>A0A452H1M4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease A-domain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pancreatic ribonuclease family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H1M4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009546.1">
        <property type="protein sequence ID" value="ENSGAGP00000008290.1"/>
        <property type="gene ID" value="ENSGAGG00000006583.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.130.10">
        <property type="entry name" value="Ribonuclease A-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001427">
        <property type="entry name" value="RNaseA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036816">
        <property type="entry name" value="RNaseA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023412">
        <property type="entry name" value="RNaseA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11437:SF10">
        <property type="entry name" value="ANGIOGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11437">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00074">
        <property type="entry name" value="RnaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00092">
        <property type="entry name" value="RNAse_Pc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54076">
        <property type="entry name" value="RNase A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019124580" description="Ribonuclease A-domain domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonuclease A-domain" evidence="6">
        <location>
            <begin position="20"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="121" mass="13711" checksum="F6D35C7CC62F3970" modified="2019-05-08" version="1">SCLSHVIFLVRVFCAYVLLHVNNPKNEAPSNRAYCKMMMVDRGVIWKYTNTFIHEPINTINAVCKDDGIPVGGFNRQSKKTFKITTCTFNILTFSFNGLNGTSKIVLTCLNGLPVRFVRYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1U9</accession>
    <name>A0A452H1U9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">3'(2'), 5'-bisphosphate nucleotidase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Inositol monophosphatase domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Myo-inositol monophosphatase A3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Phosphoadenosine phosphate 3'-nucleotidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Exhibits 3'-nucleotidase activity toward adenosine 3',5'-bisphosphate (PAP), namely hydrolyzes adenosine 3',5'-bisphosphate into adenosine 5'-monophosphate (AMP) and a phosphate. May play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3',5'-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation. Has no activity toward 3'-phosphoadenosine 5'-phosphosulfate (PAPS) or inositol phosphate (IP) substrates including I(1)P, I(1,4)P2, I(1,3,4)P3, I(1,4,5)P3 and I(1,3,4,5)P4.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine 3',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 23">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Golgi apparatus</location>
            <location evidence="16">trans-Golgi network membrane</location>
            <topology evidence="16">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.7" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452H1U9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008474"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009757.1">
        <property type="protein sequence ID" value="ENSGAGP00000008474.1"/>
        <property type="gene ID" value="ENSGAGG00000006739.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008254">
        <property type="term" value="F:3'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01640">
        <property type="entry name" value="IPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000012">
        <property type="entry name" value="Blast:Putative inositol monophosphatase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000007">
        <property type="entry name" value="Blast:Putative inositol monophosphatase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050725">
        <property type="entry name" value="CysQ/Inositol_MonoPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028:SF6">
        <property type="entry name" value="GOLGI-RESIDENT ADENOSINE 3',5'-BISPHOSPHATE 3'-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 23" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" evidence="23">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00046205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="356" mass="38740" checksum="500B2AA95A9DA855" modified="2019-05-08" version="1">MAPMGIRLSPLGVAVFCLLGLGVLYHLYSGLLASRLALFVLGAGHDVPPDTLPEGTVDLRELLAVSVLAAVRGGEEVKRVRESNVLNEKAKGKTREGAEEKMTSGDVLSNRKMFYLLKNSFPGVQINTEEHVDAPDKEIIFWDRSIPEDIKEKIQPKEVPAESVTVWIDPLDATHEYTEDLRQYVTTMVCVAVNGEPVIGVIHKPFSAYTAWAMVDGGSNVKARSSYSERTPRIIVSRSHAGKVEQVARQTFGNKTVIIPAGGAGYKVLALLDVPDKKQEKADVYIHVTYIKKWDICAGNAVLKALGGHMTTLTGEEISYTGSDGNEGGLLASINVDHQALVNKLPDLEKASHKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2E9</accession>
    <name>A0A452H2E9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H2E9"/>
    <dbReference type="SMR" id="A0A452H2E9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008586"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009872.1">
        <property type="protein sequence ID" value="ENSGAGP00000008583.1"/>
        <property type="gene ID" value="ENSGAGG00000006799.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000009875.1">
        <property type="protein sequence ID" value="ENSGAGP00000008586.1"/>
        <property type="gene ID" value="ENSGAGG00000006799.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12762">
        <property type="entry name" value="RRM1_hnRNPA2B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12581">
        <property type="entry name" value="RRM2_hnRNPA2B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000040">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein A2/B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000108">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoproteins A2/B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021662">
        <property type="entry name" value="HnRNPA1/A2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034486">
        <property type="entry name" value="hnRNPA2B1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48026:SF13">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS A2_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48026">
        <property type="entry name" value="HOMOLOGOUS TO DROSOPHILA SQD (SQUID) PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11627">
        <property type="entry name" value="HnRNPA1_LC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="32"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="123"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="200"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="317"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="331"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="364" mass="38377" checksum="76655C11452FDE78" modified="2019-05-08" version="1">MEGSKRTGSLSGPAKRRDQAGEAQMREKEQFRKLFIGGLSFETTEESLRNYYEQWGKLTDCVVMRDPASKRSRGFGFVTFSSMAEVDAAMAARPHSIDGRVVEPKRAVAREESGKPGAHVTVKKLFVGGIKEDTEEHHLRDYFEEYGKIDTIEIITDRQSGKKRGFGFVTFDDHDPVDKIVLQKYHTINGHNAEVRKALSRQEMQEVQSSRSGRGGNFGFGDSRGGGGNFGPGPGSNFRGGSDGYGSGRGFGDGYNGYGGGPGGGNFGGSPGYGGGRGGYGGGGPGYGNQGGGYGGGYDNYGGGNYGSGNYNDFGNYNQQPSNYGPMKSGNFGGSRNMGGPYGGGNYGPGGSGGSGGYGGRSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2S7</accession>
    <name>A0A452H2S7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the bZIP family. ATF subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H2S7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008929"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010259.1">
        <property type="protein sequence ID" value="ENSGAGP00000008929.1"/>
        <property type="gene ID" value="ENSGAGG00000007060.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035497">
        <property type="term" value="F:cAMP response element binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14689">
        <property type="entry name" value="bZIP_CREB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000054">
        <property type="entry name" value="Cyclic AMP-responsive element-binding protein 3-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46004">
        <property type="entry name" value="CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46004:SF1">
        <property type="entry name" value="CYCLIC AMP-RESPONSIVE ELEMENT-BINDING PROTEIN 3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00041">
        <property type="entry name" value="LEUZIPPRCREB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0834">Unfolded protein response</keyword>
    <feature type="domain" description="BZIP" evidence="11">
        <location>
            <begin position="284"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="193"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="204"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="482" mass="52597" checksum="0F524255ED8E8C1E" modified="2019-05-08" version="1">MDSILEPFPSERLFPGGGSGFLDLGDLNEADFLSNVHFSENLDHFSENMEDFSNELFSSFFDDPMLAEKNPLLDMDLDPPTPGIQAEHSYSLSGDSAPQSPLMPIKTEDNANDLESGVWSLGHKLCSIMVKQEQNLAPELPESQLAASLVPALNLNPLQRLPAPEEAPVEMTPAPVIKAEPKEVNQFLNVPADDLVQMPPTPPSSHGSDSDGSQSPRSLPPSSPARPAARSSSAISTSPLLTAPHKLQGTSGPLLLTEEEKRTLIAEGYPIPTKLPLTKAEEKALKRVRRKIKNKISAQESRRKKKEYVECLEKKVETFTSENNELWKKVETLENANRTLLQQLQKLQALVAGKVSRPYKMASTQTGTCLMVLVLCFVLVLGSMMPCLPEFSSTSQTVKAAPAPDIYTTSKSESDMTHSSNPLEVLPIPLLLIDIQQRELSHNSALSMPAWALPGRAVSQQCSVHRHRLCGCWGAGAPTGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3F7</accession>
    <name>A0A452H3F7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the actin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3F7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008952"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010285.1">
        <property type="protein sequence ID" value="ENSGAGP00000008952.1"/>
        <property type="gene ID" value="ENSGAGG00000007075.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.36.70:FF:000001">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000131">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000047">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0558">Oxidation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019363786" evidence="9">
        <location>
            <begin position="22"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="395" mass="43978" checksum="EE0B9793C45BC6EF" modified="2019-05-08" version="1">RSVLLLRRAIINLLCLLIAMADEEIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGCPRHQGVMIGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLSEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDVKEKLCYVALDFEQEMAILASSSSLEKNYELPDGQVITLGNERFRCPEALFQPVFLGMESCGIHETNFNSIMKCDVDIRKDLYANTVLSGGSTMYPGIADRMQKEIASLAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKLEYDESGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3K1</accession>
    <name>A0A452H3K1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3K1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009219"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010585.1">
        <property type="protein sequence ID" value="ENSGAGP00000009219.1"/>
        <property type="gene ID" value="ENSGAGG00000007283.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 6 REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="628"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="628"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="677"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="738"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="807" mass="88491" checksum="77925667A0772E95" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3V5</accession>
    <name>A0A452H3V5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Asparagine-linked glycosylation protein 5 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452H3V5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009295"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010676.1">
        <property type="protein sequence ID" value="ENSGAGP00000009295.1"/>
        <property type="gene ID" value="ENSGAGG00000007351.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000068">
        <property type="entry name" value="ALG5, dolichyl-phosphate beta-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="15">
        <location>
            <begin position="68"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="323" mass="36606" checksum="B990B9551BDC04A0" modified="2019-05-08" version="1">MEPLLADAVAALAALAAAALSLIIIVAHVTATKMPNFHRHEEEKFFVNAEGKKESIPSIHDPPTKELSVVVPSYNEEDRLPLMMDEALDYLEKRQKRDPAFTYEVIVVDDGSKDQTAKVAMKYCKKYGSDKVRVLTLVKNRGKGGAVRMGVFSCRGGKILMADADGATKFADIEKVEEGLRNLQPWPDQMAIACGSRAHLEQDSIAQRSYFRTLLMYGFHFLVWFLCVKEIRDTQCGFKLLTREAASQTFSALHIERWAFDVELLYIAQRLKIPIAEVAVNWTEIEGSKLVPFWSWLQMGRDLLFIRLRYLTGAWRLETSKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H438</accession>
    <name>A0A452H438_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H438"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009415"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010818.1">
        <property type="protein sequence ID" value="ENSGAGP00000009415.1"/>
        <property type="gene ID" value="ENSGAGG00000007450.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029193">
        <property type="entry name" value="TEX12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37349">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37349:SF1">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15219">
        <property type="entry name" value="TEX12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="122" mass="13948" checksum="11B29A73FD4CC4C0" modified="2019-05-08" version="1">MASNTVKVDENRSKRRKEMENEASESPQLSSLERPDLALSESSQSLYKPEALEKVLNDMNKEINNLLSKYANILSERAAMDASYVQELDGILKEARIIENHLKQKRESLKHRFTVIANTLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6L2</accession>
    <name>A0A452H6L2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NECAP PHear domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NECAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H6L2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010171"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011670.1">
        <property type="protein sequence ID" value="ENSGAGP00000010171.1"/>
        <property type="gene ID" value="ENSGAGG00000007973.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13228">
        <property type="entry name" value="PHear_NECAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000064">
        <property type="entry name" value="Adaptin ear-binding coat-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012466">
        <property type="entry name" value="NECAP_PHear"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12847:SF16">
        <property type="entry name" value="ADAPTIN EAR-BINDING COAT-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12847">
        <property type="entry name" value="ATP-BINDING CASSETTE ABC TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07933">
        <property type="entry name" value="DUF1681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NECAP PHear" evidence="8">
        <location>
            <begin position="6"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="169"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="266" mass="28539" checksum="98A0099016F8417F" modified="2019-05-08" version="1">MADSEYESVLCVKPEVHVYRVPPRASNRGYRAAEWQLDQPSWSGRLRITSKGKMAFIKLEDKNSGELFAQAPVDQFPSIAVEGVTDSSRYFVIRVEDESGRRAFIGVGFVDRGDAFDFNVAIQDHFKWVKQQSELAKQAQNPDQGPKLDLGFKEGQTIKLNIANMKKKEGAAGNSKLRPAGPGGLSLLPPPPAGRSPALIPPPGEPLPSCPADSQPTSGASTDVSLSWPQPGGATTAASADIWGDFATASGSASTQNQQSPGWVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H811</accession>
    <name>A0A452H811_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G1/S-specific cyclin-E1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for the control of the cell cycle at the G1/S (start) transition.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family. Cyclin E subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H811"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010854"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012433.1">
        <property type="protein sequence ID" value="ENSGAGP00000010854.1"/>
        <property type="gene ID" value="ENSGAGG00000008439.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20579">
        <property type="entry name" value="CYCLIN_CCNE1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20581">
        <property type="entry name" value="CYCLIN_CCNE1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000024">
        <property type="entry name" value="G1/S-specific cyclin-E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000140">
        <property type="entry name" value="G1/S-specific cyclin-E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 11" id="KW-0195">Cyclin</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="13">
        <location>
            <begin position="147"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="14">
        <location>
            <begin position="241"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="393"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="407" mass="46754" checksum="D89EEEF1F476D37E" modified="2019-05-08" version="1">MRGESDYVDEKVPSKGEGAADLAMRARKRKADVATFLQDPDEEITKMEMNKKKQYESQPSWNKDVQKSPHMLIPTPDKDEPVNIGFSHFVDLRLSPARTSPLPILGWANRDDVWKNMLNKEQTYVKDKHYMQKHPLLQPKMRTILLDWLMEVCEVYKLHRETFYLAQDFFDRFMATQQNVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACAEDEILSMELIIMKALNWNLNPLTVVSWLNIYMQVAYLNELYEVLLPQYPQQIFVQIAELLDLCVLDIGCLEYTYGVLAASALYHFSSSELMQKVSGYEWYDIEECVKWMVPFAIAIREAGSSKLKHFRGIASEDLHNIQTHINSLDLLDKAQAKQAMLAEQNRTSPLPSGVLTPPQSSKKQSTGLKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8P3</accession>
    <name>A0A452H8P3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NUC153 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H8P3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011103"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012711.1">
        <property type="protein sequence ID" value="ENSGAGP00000011103.1"/>
        <property type="gene ID" value="ENSGAGG00000008589.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039754">
        <property type="entry name" value="Esf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202:SF0">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NUC153" evidence="3">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="143" mass="17001" checksum="94DE8C2CBD40C18A" modified="2019-05-08" version="1">MALLMMDDEDDRRHFNYEKIVKQQNLSKTKKKQLMKKKELLEDDFQVNVADTRFQALYTSHLFNLDPCDPNFKKTKAVEKFLEEKARQREQKQQNLAKQIQENEIGKKENITKKAVDPALSMLIKSVKNKTEEFQARKKLKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H913</accession>
    <name>A0A452H913_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H913"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011238"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012871.1">
        <property type="protein sequence ID" value="ENSGAGP00000011238.1"/>
        <property type="gene ID" value="ENSGAGG00000008690.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001819">
        <property type="term" value="P:positive regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039499">
        <property type="entry name" value="LURA1/LRA25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037443">
        <property type="entry name" value="LURAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33767:SF2">
        <property type="entry name" value="LEUCINE RICH ADAPTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33767">
        <property type="entry name" value="LEUCINE RICH ADAPTOR PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14854">
        <property type="entry name" value="LURAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="221" mass="24516" checksum="FD825F14CD24DB04" modified="2019-05-08" version="1">MEAPDCGDPLPPDLKELESKVGRRPPEGLVRWLREDPAAALLRESPVRGQRRGLAGKIKALKLELAYLRAIDVKILQQLVVVNEGIEAVKWLLEEKGTLTSHCSSLASSQYSLVESQETSRRGSWTSLQDPGEKLDSISVGSYLDTLADEMDEPGIPPARLEQDWSRSSPDRGLPSKPASKEAQGARLSKGSPTRMLNGKLHLEYDAHWHWVQSQDDVTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H963</accession>
    <name>A0A452H963_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H963"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011122"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012737.1">
        <property type="protein sequence ID" value="ENSGAGP00000011122.1"/>
        <property type="gene ID" value="ENSGAGG00000008604.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18220">
        <property type="entry name" value="BTB_POZ_ZBTB34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000119">
        <property type="entry name" value="Zinc finger and BTB domain containing 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000422">
        <property type="entry name" value="Zinc finger and BTB domain containing 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000009">
        <property type="entry name" value="Zinc finger and BTB domain-containing 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000364">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF26">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="12">
        <location>
            <begin position="32"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="428"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="499" mass="55334" checksum="1226D6B95E6117D9" modified="2019-05-08" version="1">MDSSSFIQFDVPDYSNTVLSQLNELRLQGKLCDIIVHIQGQPFRAHKAVLAASSPYFRDHSALSTMSGLSISVIKNPNVFEQLLSFCYTGRMSLQLKDVVSFLTAASFLQMQCVIDKCTQILESIHSKISVGDVDSVTVGAEETQESHNGVKDSSYFANPVEISPPYCSQVPQPTAGSDLRMETTTGKILRSRLHEEGHSDRGSSGSVSEYEIQIEGDPEQGDLIVRESQIAEVKVKMEKSDRPSCSDSSSLGDDGYHTEMVDGEQVVAVNVGSYGSVLQHVYSFSHASSQATSMSETFGSLSNSSPSRSMLSCFRGGRARQKRVSSGHLHSDVQGLMQGADGETTVSNPGYESSPRERNARGHWYPYNERLICIYCGKSFNQKGSLDRHMRLHMGITPFVCKFCGKKYTRKDQLEYHIRGHTDDKPFRCEICGKCFPFQGTLNQHLRKNHPGVAEVRNRIESPERTEAFLEQKIDNDASVSEAMDSSMEIHTMSNTPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H983</accession>
    <name>A0A452H983_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sel-1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H983"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011132"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012747.1">
        <property type="protein sequence ID" value="ENSGAGP00000011132.1"/>
        <property type="gene ID" value="ENSGAGG00000008610.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050767">
        <property type="entry name" value="Sel1_AlgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102:SF53">
        <property type="entry name" value="PROTEIN SEL-1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102">
        <property type="entry name" value="SEL-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019415058" evidence="3">
        <location>
            <begin position="19"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="605"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="625" mass="70729" checksum="53E00B5E2ECB928F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9P9</accession>
    <name>A0A452H9P9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Defensin beta 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H9P9"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013150.1">
        <property type="protein sequence ID" value="ENSGAGP00000011487.1"/>
        <property type="gene ID" value="ENSGAGG00000008862.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019149061" description="Defensin beta 1" evidence="1">
        <location>
            <begin position="22"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="62" mass="6805" checksum="2A91E293893506D7" modified="2019-05-08" version="1">MRILYLVFAALLLVLATPGYGQYGYHKGGCAGRCAKSCAKHELTSKTYDCKHLKCCTHPKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBL5</accession>
    <name>A0A452HBL5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HBL5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012157"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013923.1">
        <property type="protein sequence ID" value="ENSGAGP00000012157.1"/>
        <property type="gene ID" value="ENSGAGG00000009354.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20201">
        <property type="entry name" value="T-box_TBX19-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000002">
        <property type="entry name" value="T-box transcription factor Brachyury"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002070">
        <property type="entry name" value="TF_Brachyury"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF114">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR TBX19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00938">
        <property type="entry name" value="BRACHYURY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="7">
        <location>
            <begin position="43"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="392" mass="43049" checksum="0F62497245B03494" modified="2019-05-08" version="1">MTDLGCKKPSDCTVSRLLNVVESELQAGRDKGDPTEKQLQLVLEEAALWQRFREVTNEMIVTKNGRRMFPVLKISVSGLDPNAMYSFLLDFAPTDGHRWKYVNGEWVPAGKPEPPSHSCVYIHPDSPNFGAHWMKASVSFSKVKLTNKLNGSGQIMLNSLHKYEPQVHIVRVGGPHRMVMNCSFPETQFIAVTAYQNEEITALKIKYNPFAKAFLDAKERNHPRDTPENVLEGQHMTYSHCKGCEHYAALRGHRAAPYPASYMQRNHSPTVNLIESSSNHLQMFPGHDSWASLPSTPHANLLSVPHAKGTATAGPSPYPCLWTVSNSALNVANPGSDMNGSAPGTFLRGNIPLPTTSASIPLQGAAASNMETPGEPALARLADSAWTSLHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HC28</accession>
    <name>A0A452HC28_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HC28"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012334"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014123.1">
        <property type="protein sequence ID" value="ENSGAGP00000012334.1"/>
        <property type="gene ID" value="ENSGAGG00000009485.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000014124.1">
        <property type="protein sequence ID" value="ENSGAGP00000012335.1"/>
        <property type="gene ID" value="ENSGAGG00000009485.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046931">
        <property type="term" value="P:pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03158">
        <property type="entry name" value="penumbra_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000007">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF154">
        <property type="entry name" value="TETRASPANIN-33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="61"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="158"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="285" mass="31775" checksum="CC99CE62CDDC09B4" modified="2019-05-08" version="1">MPRRLAGPPVGPHGEDFSFVSPLVKYLLFFFNLLFWMISMVMVAVGIYARLMKHAEAAMACLAVDPAILLIVVGILMFLITFCGCIGSLRENICLLQTFSICLTVVFLLQLAAGVLGFVFSDKARGKVSEIINNAVVHYRDDLDLQNLIDYGQKEFSCCGGISYKDWSQNMYFNCTADNPSRERCSVPYSCCLHAADQAVINTMCGQGMQAMNYLEASEFIYTNGCIDKLVNWIHSNLFLLGGIALGLAIPQLVGILLSQILISQIKDQIKLQLYNQQHRADPWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEW4</accession>
    <name>A0A452HEW4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Follistatin-related protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Follistatin-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation. Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter. Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes. Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with SCN10A. Interacts with DIP2A; DIP2A may act as a cell surface receptor for FSTL1. Interacts with BMP4. Interacts with CD14; this interaction promotes TL4-mediated signaling cascade.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HEW4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013347"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015275.1">
        <property type="protein sequence ID" value="ENSGAGP00000013347.1"/>
        <property type="gene ID" value="ENSGAGG00000010213.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030510">
        <property type="term" value="P:regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16233">
        <property type="entry name" value="EFh_SPARC_FSTL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000017">
        <property type="entry name" value="Follistatin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057020">
        <property type="entry name" value="EF-hand_FSTL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003645">
        <property type="entry name" value="Fol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015369">
        <property type="entry name" value="Follistatin/Osteonectin_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050653">
        <property type="entry name" value="Prot_Inhib_GrowthFact_Antg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913">
        <property type="entry name" value="FOLLISTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913:SF13">
        <property type="entry name" value="FOLLISTATIN-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23564">
        <property type="entry name" value="EF-hand_FSTL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09289">
        <property type="entry name" value="FOLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23244">
        <property type="entry name" value="VWF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00274">
        <property type="entry name" value="FOLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Kazal-like" evidence="13">
        <location>
            <begin position="71"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="381" mass="43206" checksum="921F7F23132B67AB" modified="2019-05-08" version="1">PGSACLRRTETSCSWRVSSCRGGPWQQWQLGSSGPCWRQEEPKSKSKICANVFCGAGRECAVTEKGEPSCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKERKSENPAASPVVCYQSDRDELRRRIIQWLEAEIVPDGWFSKGSNYSEVLDKYFKSFDGGDSRLDSNEFLKFVEQNETAINITTYVDQETNKLLRGLCVDALIELSDENADWKLSFNEFLKCLNPSFNPPEKKCALEDETYEDGAETQVECNRCVCACGNWVCTAMTCEGKSQKVPVGGQQPEEELTEEELARYVQELQKHQVRLESLCPESVWKFLLARDLLPLYLRYFLAKRGSTLAFGRGELPFSLWDGPSMAGARHIRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HF86</accession>
    <name>A0A452HF86_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SOCS box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HF86"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013492"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015446.1">
        <property type="protein sequence ID" value="ENSGAGP00000013492.1"/>
        <property type="gene ID" value="ENSGAGG00000010309.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF176">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX CONTAINING 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="118"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="151"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="217"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="315"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="392"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="5">
        <location>
            <begin position="531"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="589" mass="65723" checksum="6AC98BA3E7EFB50E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHB1</accession>
    <name>A0A452HHB1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Doublecortin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HHB1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014270"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016331.1">
        <property type="protein sequence ID" value="ENSGAGP00000014270.1"/>
        <property type="gene ID" value="ENSGAGG00000010859.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902412">
        <property type="term" value="P:regulation of mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17157">
        <property type="entry name" value="DCX2_DCDC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17155">
        <property type="entry name" value="DCX_DCDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.230">
        <property type="entry name" value="Doublecortin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043188">
        <property type="entry name" value="DCDC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056415">
        <property type="entry name" value="DCX2_DCDC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003533">
        <property type="entry name" value="Doublecortin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036572">
        <property type="entry name" value="Doublecortin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46302">
        <property type="entry name" value="DOUBLECORTIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46302:SF3">
        <property type="entry name" value="DOUBLECORTIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24478">
        <property type="entry name" value="DCX2_DCDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89837">
        <property type="entry name" value="Doublecortin (DC)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50309">
        <property type="entry name" value="DC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Doublecortin" evidence="2">
        <location>
            <begin position="258"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="595" mass="66240" checksum="938BF635BFB4FFFE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HI38</accession>
    <name>A0A452HI38_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Growth/differentiation factor 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Bone morphogenetic protein 14</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction. Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG. Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HI38"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014572"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016669.1">
        <property type="protein sequence ID" value="ENSGAGP00000014572.1"/>
        <property type="gene ID" value="ENSGAGG00000011070.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007178">
        <property type="term" value="P:cell surface receptor protein serine/threonine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032332">
        <property type="term" value="P:positive regulation of chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19399">
        <property type="entry name" value="TGF_beta_GDF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000001">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000011">
        <property type="entry name" value="Growth/differentiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF44">
        <property type="entry name" value="GROWTH_DIFFERENTIATION FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00669">
        <property type="entry name" value="INHIBINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0339">Growth factor</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019566212" description="Growth/differentiation factor 5" evidence="17">
        <location>
            <begin position="32"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="18">
        <location>
            <begin position="377"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="31"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="86"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="103"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="500" mass="56306" checksum="AF9163F4D5015468" modified="2019-05-08" version="1">MKISHFLTLLLWHLTWFYLVLVSVVLSNSEASQSNPGSRLGLLKTEGKETNPLPRAGTFRTGNHGYSTGTLKARAKSNTAQAAALLAKNNESKKSLSREGTTDTKAGQSPNRQSGVRTVTPKVQNLGSKASLRKTGTGNIDASSYKTKKTKEPVTLREPKETFKHPPITPHEYMLSLYRTLSDAERKGINGSVKLETGLANTITSFIDKGQDDRAPAIRKQKYVFDISALEKDGLLGAELRILRKKPSETWKPHSPGKTSQVKLFSCPTNRQAATLLDSRTVSIADTPKWEVFDIWKLFRNFKNSANLCFELEAFDRGRAIDLRSMGLNRTGRQVNEKALFLVFGRTKKRDLFFNEIKARSGQDDKTVYEYLFNQRRKRRAPLATRQGKRPNKNLKARCSKKALHVNFKDMGWDDWIIAPLEYEAYHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HI68</accession>
    <name>A0A452HI68_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HI68"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014591"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016690.1">
        <property type="protein sequence ID" value="ENSGAGP00000014591.1"/>
        <property type="gene ID" value="ENSGAGG00000011086.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019815">
        <property type="term" value="C:B cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030183">
        <property type="term" value="P:B cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050853">
        <property type="term" value="P:B cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14334">
        <property type="entry name" value="B-CELL ANTIGEN RECEPTOR COMPLEX-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14334:SF2">
        <property type="entry name" value="B-CELL ANTIGEN RECEPTOR COMPLEX-ASSOCIATED PROTEIN BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="9"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="202" mass="22427" checksum="E1AE87EF913AA325" modified="2019-05-08" version="1">SQKTLWELPTDVVSQEPRSLRTTAGENVTLSCTFQNRNGSRAKIIWLRGSGEDLELDSNHPFYRGQLRVFRMDEHRQGKATLTLAELQERDSGLYQCCIKLDHGETVMGGGTELRVMWRHLSATGESRGQGMGPVPSRRWGRELCRDSGPLFYRAVISLALVVLFSLGIVLSLRPWKGTGSARVPPSLPIPNGYMASLCGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIY3</accession>
    <name>A0A452HIY3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ciliary microtubule inner protein 2A-C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CIMIP2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HIY3"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016951.1">
        <property type="protein sequence ID" value="ENSGAGP00000014836.1"/>
        <property type="gene ID" value="ENSGAGG00000011234.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052683">
        <property type="entry name" value="CIMIP2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018902">
        <property type="entry name" value="CMI2A-C-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47299">
        <property type="entry name" value="PROTEIN FAM166A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47299:SF1">
        <property type="entry name" value="PROTEIN FAM166A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10629">
        <property type="entry name" value="CMI2B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ciliary microtubule inner protein 2A-C-like" evidence="6">
        <location>
            <begin position="13"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="227" mass="25147" checksum="D145095FF692FFAB" modified="2019-05-08" version="1">MAAPRKHGLFTPDPYYIPGYGGFYPQIRYQIGETYGKTTSRLLTDPSVLKSPCSVLAPLVKPRFLEDFSGTKHPSNQMLDQNYSYFPGYTGFIPYQNAQFGPYYPTPATTSRAPLLMLLPAQVQQSWVDSDTGDSTQLREQRKYIPCHPGIRLACGHGWKSSSSPTGMHLASEQEKRKAPCYPDIALACGQQKNHGPCHPDVALACGQGWRSSPCLRGTKQVCGRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJ89</accession>
    <name>A0A452HJ89_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJ89"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014940"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017066.1">
        <property type="protein sequence ID" value="ENSGAGP00000014940.1"/>
        <property type="gene ID" value="ENSGAGG00000011290.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042269">
        <property type="term" value="P:regulation of natural killer cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03593">
        <property type="entry name" value="CLECT_NK_receptors_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051527">
        <property type="entry name" value="KLR_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033992">
        <property type="entry name" value="NKR-like_CTLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46784">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR SUBFAMILY B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46784:SF1">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR SUBFAMILY B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="C-type lectin" evidence="6">
        <location>
            <begin position="100"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="215" mass="24247" checksum="1A21953D9FC9DA89" modified="2019-05-08" version="1">MTEEIIYTDINVLEDPSPACQPSPLKHHVFQGRSLQTDTDAVSSVPKNSSVAQRHCPGRECNRSLEDFVSRLKQSLCHSAQSPTADSSSCKLCPMDWLSHRGKCFWFSKDSKNWTESRADCSAKSSRKLVIQDQEEMEFIQNVTRGKYHVWLGLSVTSAEKNWTWLDSSVLDQTLFPVTGPTDVNSCGVIKGNRIHSETCSAEFKWICQKEAMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJT6</accession>
    <name>A0A452HJT6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL34</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L34</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJT6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015176"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017333.1">
        <property type="protein sequence ID" value="ENSGAGP00000015176.1"/>
        <property type="gene ID" value="ENSGAGG00000011457.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.370.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038562">
        <property type="entry name" value="Ribosomal_eL34_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_eL34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46595">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="117" mass="13309" checksum="FC11B00B98CB770C" modified="2019-05-08" version="1">MVQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLKAQAQSQKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HM16</accession>
    <name>A0A452HM16_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HM16"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015970"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018248.1">
        <property type="protein sequence ID" value="ENSGAGP00000015970.1"/>
        <property type="gene ID" value="ENSGAGG00000012015.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000076">
        <property type="entry name" value="Leucine-rich repeat and Ig domain-containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000014">
        <property type="entry name" value="Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF207">
        <property type="entry name" value="LEUCINE RICH REPEAT AND IG DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019289136" description="Ig-like domain-containing protein" evidence="12">
        <location>
            <begin position="27"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="540"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="409"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="605" mass="68268" checksum="F5C86849C55B9F0B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNQ0</accession>
    <name>A0A452HNQ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SNARE-associated protein Snapin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Biogenesis of lysosome-related organelles complex 1 subunit 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Synaptic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SNAPIN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HNQ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016613"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018963.1">
        <property type="protein sequence ID" value="ENSGAGP00000016613.1"/>
        <property type="gene ID" value="ENSGAGG00000012425.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000300">
        <property type="term" value="P:regulation of synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016079">
        <property type="term" value="P:synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017246">
        <property type="entry name" value="Snapin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028119">
        <property type="entry name" value="Snapin/Pallidin/Snn1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31305">
        <property type="entry name" value="SNARE-ASSOCIATED PROTEIN SNAPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31305:SF2">
        <property type="entry name" value="SNARE-ASSOCIATED PROTEIN SNAPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14712">
        <property type="entry name" value="Snapin_Pallidin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037631">
        <property type="entry name" value="Snapin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0770">Synapse</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="80"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR037631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="132" mass="14553" checksum="B448D55E835456C1" modified="2019-05-08" version="1">MAALAGPGAGTPGSGARDLFAEGLLEFLRPAARQLDSHVHAVRESQVELREHIDNLATELCRINEDQKVALDLDPYVKKLLNARRRVVLVNNILQNAQERLRRLNHNVAKETARRKAILEASGGYPQGSPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNZ5</accession>
    <name>A0A452HNZ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">V-set and immunoglobulin domain containing 10 like 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HNZ5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016691"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019057.1">
        <property type="protein sequence ID" value="ENSGAGP00000016691.1"/>
        <property type="gene ID" value="ENSGAGG00000012467.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052598">
        <property type="entry name" value="IgSF_CEA-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337:SF20">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="656"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="200"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="295"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="472"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="563"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="270"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="270"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="718" mass="74784" checksum="1AFC2F46D627968B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQU6</accession>
    <name>A0A452HQU6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Prealbumin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HQU6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017421"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019872.1">
        <property type="protein sequence ID" value="ENSGAGP00000017421.1"/>
        <property type="gene ID" value="ENSGAGG00000011511.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160064">
        <property type="term" value="C:multi-pass translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160063">
        <property type="term" value="P:multi-pass transmembrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055073">
        <property type="entry name" value="NOMO1-like_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056191">
        <property type="entry name" value="NOMO_12th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056190">
        <property type="entry name" value="NOMO_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056188">
        <property type="entry name" value="NOMO_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056319">
        <property type="entry name" value="NOMO_7th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056187">
        <property type="entry name" value="NOMO_8th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051417">
        <property type="entry name" value="SDr/BOS_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041033">
        <property type="entry name" value="SpaA_PFL_dom_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23303:SF14">
        <property type="entry name" value="BOS COMPLEX SUBUNIT NOMO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23303">
        <property type="entry name" value="CARBOXYPEPTIDASE REGULATORY REGION-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23141">
        <property type="entry name" value="Ig_NOMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22902">
        <property type="entry name" value="NOMO1-like_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23192">
        <property type="entry name" value="NOMO_12th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23194">
        <property type="entry name" value="NOMO_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23196">
        <property type="entry name" value="NOMO_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23660">
        <property type="entry name" value="NOMO_8th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17802">
        <property type="entry name" value="SpaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="NOMO fifth transthyretin-like" evidence="7">
        <location>
            <begin position="92"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO sixth transthyretin-like" evidence="8">
        <location>
            <begin position="183"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO seventh transthyretin-like" evidence="5">
        <location>
            <begin position="276"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO eighth prealbumin-like" evidence="9">
        <location>
            <begin position="426"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO-like ninth beta-sandwich" evidence="4">
        <location>
            <begin position="480"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="SpaA-like prealbumin fold" evidence="3">
        <location>
            <begin position="555"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO C-terminal transthyretin-like" evidence="6">
        <location>
            <begin position="735"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="386"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="932" mass="103116" checksum="4D86C045F057EA9C" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HR20</accession>
    <name>A0A452HR20_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Reelin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Extracellular matrix serine protease secreted by pioneer neurons that plays a role in layering of neurons in the cerebral cortex and cerebellum by coordinating cell positioning during neurodevelopment. Regulates microtubule function in neurons and neuronal migration. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Oligomer of disulfide-linked homodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the reelin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000017473"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019928.1">
        <property type="protein sequence ID" value="ENSGAGP00000017473.1"/>
        <property type="gene ID" value="ENSGAGG00000012984.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000019949.1">
        <property type="protein sequence ID" value="ENSGAGP00000017493.1"/>
        <property type="gene ID" value="ENSGAGG00000012984.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070325">
        <property type="term" value="F:lipoprotein particle receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10037">
        <property type="entry name" value="Reelin_repeat_1_subrepeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10045">
        <property type="entry name" value="Reelin_repeat_1_subrepeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10038">
        <property type="entry name" value="Reelin_repeat_2_subrepeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10046">
        <property type="entry name" value="Reelin_repeat_2_subrepeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10039">
        <property type="entry name" value="Reelin_repeat_3_subrepeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10047">
        <property type="entry name" value="Reelin_repeat_3_subrepeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10040">
        <property type="entry name" value="Reelin_repeat_4_subrepeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10048">
        <property type="entry name" value="Reelin_repeat_4_subrepeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10041">
        <property type="entry name" value="Reelin_repeat_5_subrepeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10049">
        <property type="entry name" value="Reelin_repeat_5_subrepeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10042">
        <property type="entry name" value="Reelin_repeat_6_subrepeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10050">
        <property type="entry name" value="Reelin_repeat_6_subrepeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10043">
        <property type="entry name" value="Reelin_repeat_7_subrepeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10051">
        <property type="entry name" value="Reelin_repeat_7_subrepeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10044">
        <property type="entry name" value="Reelin_repeat_8_subrepeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10052">
        <property type="entry name" value="Reelin_repeat_8_subrepeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10036">
        <property type="entry name" value="Reelin_subrepeat_Nt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000003">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000028">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000030">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000036">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000039">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000040">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000041">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000042">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000044">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000045">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000047">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000052">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000053">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000055">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000056">
        <property type="entry name" value="reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000057">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034968">
        <property type="entry name" value="Reelin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049419">
        <property type="entry name" value="Reelin_subrepeat-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036278">
        <property type="entry name" value="Sialidase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11841">
        <property type="entry name" value="REELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11841:SF1">
        <property type="entry name" value="REELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21471">
        <property type="entry name" value="Reelin_subrepeat-B"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50939">
        <property type="entry name" value="Sialidases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="15 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 21" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="1935"/>
            <end position="1967"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="3034"/>
            <end position="3066"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="1939"/>
            <end position="1949"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="1957"/>
            <end position="1966"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="3038"/>
            <end position="3048"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="3056"/>
            <end position="3065"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00046064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="3234" mass="363907" checksum="AA6B4564877E9BE5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRK9</accession>
    <name>A0A452HRK9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HRK9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017687"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020181.1">
        <property type="protein sequence ID" value="ENSGAGP00000017687.1"/>
        <property type="gene ID" value="ENSGAGG00000013141.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03872">
        <property type="entry name" value="M14_CPA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000001">
        <property type="entry name" value="Carboxypeptidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033843">
        <property type="entry name" value="CPAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF18">
        <property type="entry name" value="CARBOXYPEPTIDASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M14" evidence="12">
        <location>
            <begin position="108"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="408" mass="47508" checksum="A28327A8B33D18A7" modified="2019-05-08" version="1">LYNVENLIQKSGGYLNISWIMMPGNSDQEEVKKIVDLWQPSSIFHISEGTVTDVRVPGNSSQILLPYLQQANIKYTIIISDLQKAIEKQSRLRSCRNRRSVPGYNYEVYHSLEEIQSWMYRLNKTHSDLIHMFSVGKSYEGRPLFVLKLGKRSRPYKRAVWIDCGIHAREWIGPAFCQWFVKEALQTYQSDPAMRRMLNQLYFYIMPVFNVDGYHFSWTHDRFWRKTRSKNSQFHCHGVDANRNWKVKWCDEGASFHPCDDTYCGPFPESEPEVKAVAHFLRKHRKQIKAYLSFHAYAQMLLYPYSYKYATIPNFSCVESAAYNAVNALQSAYGIRYRYGPASSTLYVSSGSSMDWAYKNGIPYAFAFELRDTGHFGFLLPEALIKPTCTETMLAVKNITMHLLKKCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRV7</accession>
    <name>A0A452HRV7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM114 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HRV7"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020319.1">
        <property type="protein sequence ID" value="ENSGAGP00000017808.1"/>
        <property type="gene ID" value="ENSGAGG00000013221.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000020322.1">
        <property type="protein sequence ID" value="ENSGAGP00000017810.1"/>
        <property type="gene ID" value="ENSGAGG00000013221.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007998">
        <property type="entry name" value="DUF719"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12842">
        <property type="entry name" value="FI01459P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12842:SF3">
        <property type="entry name" value="PROTEIN FAM114A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05334">
        <property type="entry name" value="DUF719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="119"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="502" mass="53844" checksum="6A5E6910CCD6F559" modified="2019-05-08" version="1">MSEKAGSESLKAGASQKDQNGQRAEEAGSVESSGREEDESEPVALTRKRPEAKPSNEPIVAEEPVDQALKVSDSTDASQTGWGYWGSWGKSLLSTASATVATVGQGISNVIEKAETSLGIPSPSEISSEAKVVTGGSQSSEASSTGGVDDSSSSPISGAFGVLSTLSTAVQSTGKSVITGGLDALEFIGKKTMDVIAEGDPGFKKTKGLMNRNSTLSQVLREAKEKEEQRTATEVTMATEKKAHYGLLFDEFQGLSHLEALEMLSRESDSKVKSVINALSGEELDMLKVELEQLKEAFSLAEFYDDEEEEKKGDEDFTKQITALFFELHISAKPDKLAAARKSAHEWIERLNVEAPKEGKMSEENQQLDSGDANQDVQTSVEDIHAFAIRSLAELTACSIEMFHKTAALVLHGQKQEVSATGRAKALSQMTIVLCKELSSLSKEFTTCLTTAGVKEKADVLNPLITGVFLEASNSASYIQDAFQLLLPVLQISLVQTRTELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSM7</accession>
    <name>A0A452HSM7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pepsin A</fullName>
            <ecNumber evidence="3">3.4.23.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452HSM7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018050"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020585.1">
        <property type="protein sequence ID" value="ENSGAGP00000018050.1"/>
        <property type="gene ID" value="ENSGAGG00000013354.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000006">
        <property type="entry name" value="Cathepsin E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000004">
        <property type="entry name" value="Pepsin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012848">
        <property type="entry name" value="Aspartic_peptidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF22">
        <property type="entry name" value="PEPSIN A-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07966">
        <property type="entry name" value="A1_Propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-0222">Digestion</keyword>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="12">
        <location>
            <begin position="88"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="119"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="280"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="392" mass="42134" checksum="7E9BA2DAADC752E9" modified="2019-05-08" version="1">MCLLIRSLAVLNGKFAYCLFVIDWSLSPPLLIRVSLKKGKSLRQNLKEHGLLEDFLKKNPYNLASKYFPSLANDAASEPLTNYMDIEYYGTISIGTPAQEFSVLFDTGSSNLWVPSVYCSSTACTNHNRFNPSDSSTYEATNESLSIEYGSGSMTGILAYDTVRVGGIVDTKQMFGLSETEPGTAFEYSEFDGILGLAFPSISASGATPVFDNMMNEGLVSQDLFSVYLSSDEQSGSFVMFGGIDSSYYSGSLNWIPLSAETYWEITMDSITINGETIACSGGCQAIIDTGTSLLAGPSTGISNIESYIGASDGTISCSAISSLPNIVFTINDIEFPVPPSAYILDVTTTQAGSIDLPTSSGELWILGDVFISQYYVVFDRANNKVALASVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTN7</accession>
    <name>A0A452HTN7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000018456"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021032.1">
        <property type="protein sequence ID" value="ENSGAGP00000018456.1"/>
        <property type="gene ID" value="ENSGAGG00000013661.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051645">
        <property type="term" value="P:Golgi localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01253">
        <property type="entry name" value="PH_ARHGAP21-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04395">
        <property type="entry name" value="RhoGAP_ARHGAP21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000014">
        <property type="entry name" value="Rho GTPase activating protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000101">
        <property type="entry name" value="Rho GTPase activating protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23175">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23175:SF16">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="704"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="14">
        <location>
            <begin position="924"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="78"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="422"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="559"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="632"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="679"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="835"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="449"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="565"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="648"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="835"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="853"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="876"/>
            <end position="899"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1223" mass="137393" checksum="DA34A142879E2536" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HW78</accession>
    <name>A0A452HW78_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TBC1 domain family member 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Akt substrate of 160 kDa</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HW78"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019414"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022111.1">
        <property type="protein sequence ID" value="ENSGAGP00000019414.1"/>
        <property type="gene ID" value="ENSGAGG00000013759.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01269">
        <property type="entry name" value="PTB_TBC1D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000003">
        <property type="entry name" value="Putative TBC1 domain family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000001">
        <property type="entry name" value="TBC1 domain family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2750:FF:000002">
        <property type="entry name" value="TBC1 domain family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000076">
        <property type="entry name" value="TBC1 domain family member 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021785">
        <property type="entry name" value="DUF3350"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF14">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11830">
        <property type="entry name" value="DUF3350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PID" evidence="12">
        <location>
            <begin position="167"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="13">
        <location>
            <begin position="758"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="453"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="575"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="623"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="693"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1039"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="465"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="515"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="577"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="624"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1138" mass="129068" checksum="9D504ED5322EFE61" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HW80</accession>
    <name>A0A452HW80_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">guanidinoacetate N-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis; creatine biosynthesis; creatine from L-arginine and glycine: step 2/2.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.2" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452HW80"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019374"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022068.1">
        <property type="protein sequence ID" value="ENSGAGP00000019374.1"/>
        <property type="gene ID" value="ENSGAGG00000014295.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000022070.1">
        <property type="protein sequence ID" value="ENSGAGP00000019375.1"/>
        <property type="gene ID" value="ENSGAGG00000014295.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030731">
        <property type="term" value="F:guanidinoacetate N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006601">
        <property type="term" value="P:creatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000096">
        <property type="entry name" value="Guanidinoacetate N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051038">
        <property type="entry name" value="RMT2/GAMT_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026480">
        <property type="entry name" value="RMT2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379:SF1">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51559">
        <property type="entry name" value="SAM_RMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RMT2" evidence="7">
        <location>
            <begin position="65"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="290" mass="32754" checksum="156E1A8F5ED1CB42" modified="2019-05-08" version="1">MYSSPSVCISRHTGETVRAQPDESGAPCPQISHLPRRHQACWPFRCGGSTSRSMSSSEAARPIFLQGENCKPSWKEADAGFNQTDTHLEILGKPVMERWETPYMHSLATVAASKGGCVLEVGFGMAIAATKLEEFDIEEHWIIECNEGVFQRLQEWAKKQPHKVVPLKGLWEDVAPTLPDGHFNGILYDTYPLSAETWHTHQFSFIKGHAFRLLKAGGVLTYCNLTSWGELLKTEYTDIEKMFEETQVAHLMEAGFKKENISTSVMDLIPPEDCRYYSFHKMITPTILKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HX49</accession>
    <name>A0A452HX49_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Immunoglobulin I-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HX49"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019788"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022548.1">
        <property type="protein sequence ID" value="ENSGAGP00000019788.1"/>
        <property type="gene ID" value="ENSGAGG00000014591.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015631">
        <property type="entry name" value="CD2/SLAM_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12080">
        <property type="entry name" value="SIGNALING LYMPHOCYTIC ACTIVATION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019088618" description="Immunoglobulin I-set domain-containing protein" evidence="7">
        <location>
            <begin position="19"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin I-set" evidence="8">
        <location>
            <begin position="44"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="236"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="323" mass="35903" checksum="1128773A983838FB" modified="2019-05-08" version="1">SFAFQFTLCALIILQVHSDVTTVNEILGGFVTFHLDYPDFEYPAHITWKKGNTLLHNRTYHRLTISSNGTLALKNIEKKDEGQYTASVYNITGNLVYQRIFQLYIHEPTQIPAVHVSGHSDETDLLCERANRSGLRFHWTLNGHPVDLTETEDDGKRIILAKEVAGKLDCQIHHKNSTNECSIIELKCKERDLLQQPLFLYTLAACGGGAIVLAVIASLITCCCLKSKQQFIPVPSEEEKEEGMTMSVISNEGTKSPPNGDHHEAPSAPDDSPPNPESTETCQDLEAEPKMEAASEVTPDPEVVVDINNEEFLCDSFPDPIDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXE9</accession>
    <name>A0A452HXE9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Neurogenic differentiation factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HXE9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019817"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022582.1">
        <property type="protein sequence ID" value="ENSGAGP00000019817.1"/>
        <property type="gene ID" value="ENSGAGG00000014608.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000006">
        <property type="entry name" value="Neurogenic differentiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022575">
        <property type="entry name" value="NeuroD_DUF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016637">
        <property type="entry name" value="TF_bHLH_NeuroD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF83">
        <property type="entry name" value="NEUROGENIC DIFFERENTIATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12533">
        <property type="entry name" value="Neuro_bHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015618">
        <property type="entry name" value="bHLH_NeuroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="1 9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2 9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="11">
        <location>
            <begin position="104"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="356" mass="40138" checksum="1A2E0869E5A2C9B4" modified="2019-05-08" version="1">MLTRLFSEPSLLPEVQKFPGWAEECEEDEPSDKEGRKGKTCPQQEEPREGSLGESKEEGDLGGDEEEEEEEEEEGVEEAEGDRPKKRGPKKRKMTKARLERSKLRRQKANARERNRMHDLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEILRSGKRPDLVSYVQTLCKGLSQPTTNLVAGCLQLNSRNFLTEQGQEAARYHGPNSSFALHPYAYQCSRLSGTPCQSGPMASSHALRTHTYCASYETLYGNASPDYNSSEYDAPLSPPLCINGNFSLKQDSSPDHEKNYHYSMHYSALPSSRPAGHNLVFGSSGVRGGVHSENILPYDMHLHHDRGPMYEELNAFFHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HY55</accession>
    <name>A0A452HY55_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endothelin-like toxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the endothelin/sarafotoxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HY55"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020057"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022846.1">
        <property type="protein sequence ID" value="ENSGAGP00000020057.1"/>
        <property type="gene ID" value="ENSGAGG00000014777.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031708">
        <property type="term" value="F:endothelin B receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003100">
        <property type="term" value="P:regulation of systemic arterial blood pressure by endothelin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019229">
        <property type="term" value="P:regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014826">
        <property type="term" value="P:vein smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020475">
        <property type="entry name" value="Endothelin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019764">
        <property type="entry name" value="Endothelin_toxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001928">
        <property type="entry name" value="Endothln-like_toxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13874">
        <property type="entry name" value="ENDOTHELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13874:SF9">
        <property type="entry name" value="ENDOTHELIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00322">
        <property type="entry name" value="Endothelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00365">
        <property type="entry name" value="ENDOTHELIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00272">
        <property type="entry name" value="END"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00270">
        <property type="entry name" value="ENDOTHELIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0838">Vasoactive</keyword>
    <keyword evidence="5" id="KW-0839">Vasoconstrictor</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019126797" description="Endothelin-like toxin domain-containing protein" evidence="6">
        <location>
            <begin position="25"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Endothelin-like toxin" evidence="7">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Endothelin-like toxin" evidence="7">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="183" mass="20469" checksum="43F7A9EC095941C3" modified="2019-05-08" version="1">MVNHPAGFFSLAITLCILLGEGMGRPPLESHLAAISSRHPRTKRCSCNSWQDKECIYFCHLDIIWVNTPGQTAPYGLGSPPRRRKRSLNRCECSHSKDSICATFCQGWYSPRFYPSAGQGMLCKGCTLAREQMGVQDVKWKPTERRAQYLSAFGRAVIASQASFKRDGLRQKPGSQPRAVLEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZZ6</accession>
    <name>A0A452HZZ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HDGF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZZ6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020830"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023725.1">
        <property type="protein sequence ID" value="ENSGAGP00000020830.1"/>
        <property type="gene ID" value="ENSGAGG00000015328.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20151">
        <property type="entry name" value="PWWP_PSIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000017">
        <property type="entry name" value="hepatoma-derived growth factor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.10:FF:000005">
        <property type="entry name" value="PC4 and SFRS1-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036218">
        <property type="entry name" value="HIVI-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021567">
        <property type="entry name" value="LEDGF_IBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550">
        <property type="entry name" value="HEPATOMA-DERIVED GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550:SF42">
        <property type="entry name" value="PC4 AND SFRS1-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11467">
        <property type="entry name" value="LEDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140576">
        <property type="entry name" value="HIV integrase-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PWWP" evidence="9">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="110"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="491"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="255"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="333"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="351"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="491"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="543"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="579" mass="65630" checksum="D87B4C40735F8B19" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0F3</accession>
    <name>A0A452I0F3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">P-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I0F3"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023904.1">
        <property type="protein sequence ID" value="ENSGAGP00000020986.1"/>
        <property type="gene ID" value="ENSGAGG00000015423.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019085947" description="P-type domain-containing protein" evidence="3">
        <location>
            <begin position="16"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="4">
        <location>
            <begin position="24"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="62" mass="6898" checksum="E33A9BF77F5F4F9B" modified="2019-05-08" version="1">MVIQILALLLSLGRSTETDLKPKRQCAVSSKERKDCGKLGINAVECQTKRYCFDASIKGVPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0L9</accession>
    <name>A0A452I0L9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SOWAHA-C winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the SOWAH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I0L9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021009"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023930.1">
        <property type="protein sequence ID" value="ENSGAGP00000021009.1"/>
        <property type="gene ID" value="ENSGAGG00000015438.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058889">
        <property type="entry name" value="WHD_SOWAHA-C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491:SF4">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN SOWAHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491">
        <property type="entry name" value="SOSONDOWAH, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25877">
        <property type="entry name" value="WHD_SOWAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SOWAHA-C winged helix-turn-helix" evidence="6">
        <location>
            <begin position="7"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="77"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="375"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="459" mass="49614" checksum="4A10FADAD270FF4E" modified="2019-05-08" version="1">MAEEPALSSESVLRFLAARGGRARNTELLEHFRGWLSPPEPERRARARQRFKELVNAVATVRLEPGSGAKYVQLRRRYRHGGTPAANKPDSPPGDRPLEPGDSETAAPLDPRAAPGCPQQPPQAGSGSDLPRLGQGAEAGSGSPYSPGSSGGGATPRSGRKNFRELMMGSSPQLKRSFFPSAPRARGGDSDSASLASSTAEEESGGGSSVALDPLEHAWMLSAADGRWESLEGLLSSEPGLLSKRDFITGFTCLHWAAKHGRHELLAMLVNFAHKQRLPININARTSGGYTALHLAAMHGHLEVVKLLVGAYDADVDVRDYSGRKASNYLSQGTTEEMRSLVGGLQEEGEGTVGNGSGRWRLSKVLPSNLITHKLSHHHQGQGEETESSEVAAAQGREMSRKASNSGRMKPRLNKIRFRTQIIHTTPSFRGEAEEEEEEQEEKPLKTSFKLRPKSNVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0W1</accession>
    <name>A0A452I0W1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>12-(9Z-hexadecenoyloxy)-octadecanoate + H2O = 12-hydroxyoctadecanoate + (9Z)-hexadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52072"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136312"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:52073"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>12-(9Z-octadecenoyloxy)-octadecanoate + H2O = 12-hydroxyoctadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52060"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136302"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:52061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>12-hexadecanoyloxy-octadecanoate + H2O = 12-hydroxyoctadecanoate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52056"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83677"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:52057"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>12-octadecanoyloxy-octadecanoate + H2O = 12-hydroxyoctadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136330"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:52081"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>13-(9Z-hexadecenoyloxy)-octadecanoate + H2O = 13-hydroxy-octadecanoate + (9Z)-hexadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52076"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
            <dbReference type="ChEBI" id="CHEBI:136315"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:52077"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>13-(9Z-octadecenoyloxy)-octadecanoate + H2O = 13-hydroxy-octadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:136303"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:52065"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>13-octadecanoyloxy-octadecanoate + H2O = 13-hydroxy-octadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52084"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
            <dbReference type="ChEBI" id="CHEBI:136335"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:52085"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>9-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-hexadecenoate + 9-hydroxy-octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52068"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
            <dbReference type="ChEBI" id="CHEBI:136309"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:52069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>9-(9Z-octadecenoyloxy)-octadecanoate + H2O = 9-hydroxy-octadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52048"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:136282"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:52049"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>9-hexadecanoyloxy-octadecanoate + H2O = 9-hydroxy-octadecanoate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52052"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83670"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:52053"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>9-octadecanoyloxy-octadecanoate + H2O = 9-hydroxy-octadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
            <dbReference type="ChEBI" id="CHEBI:136373"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:52097"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIG1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I0W1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021101"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024036.1">
        <property type="protein sequence ID" value="ENSGAGP00000021101.1"/>
        <property type="gene ID" value="ENSGAGG00000015499.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006838">
        <property type="entry name" value="ADTRP_AIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989:SF11">
        <property type="entry name" value="ANDROGEN-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989">
        <property type="entry name" value="ANDROGEN-INDUCED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04750">
        <property type="entry name" value="Far-17a_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="238" mass="27424" checksum="6FE04A730F335D07" modified="2019-05-08" version="1">MALVPCQVLRVAILLSYFSILCAYNAIDMPAHQTYGGSWKFLTFIDLVIQAVFFGICVLTDLSSLLTKGSDSQEQERQLKKLISLRDWIMAVLAFPIGVFVVTMFWSLYIYDREVVYPKLLDNFIPSWLNHGMHTTVLPFILIEMRTTHHQYPSRSCGLAAVCTFSVGYILWVCWIHQVTGVWVYPLLDYISPGAKIIFFATVTLVLNIFYLLGEVLNNYIWDTQKCIEERKEKPKMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2X0</accession>
    <name>A0A452I2X0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">M-phase inducer phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the MPI phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452I2X0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021857"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024899.1">
        <property type="protein sequence ID" value="ENSGAGP00000021857.1"/>
        <property type="gene ID" value="ENSGAGG00000015997.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01530">
        <property type="entry name" value="Cdc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000004">
        <property type="entry name" value="M-phase inducer phosphatase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000751">
        <property type="entry name" value="MPI_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF48">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06617">
        <property type="entry name" value="M-inducer_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00716">
        <property type="entry name" value="MPIPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5 7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="9">
        <location>
            <begin position="257"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="161"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="406" mass="47324" checksum="5E09E1FCF0ADB419" modified="2019-05-08" version="1">KNISNAKELGSPETHDRPLRRKENKENAGFVFKIPLRPTNHCRLHPFDGSSERDPFAQRPNSAPDLLCGTPEKENMGVEAESPVCLRRSSLTSFVTNEEDDGFMEILDEEELKVQRARMVPHGSNRSSLSSVQRSVRPSKCRQLFRSPSMPSSVIRPILKRLDRPQDKDTPVKTKRRKSVAGTPIEEKAEEPKSRLLRSRSFCHDEIENILDNDHRELIGDFSKAYLLQTVEGRHQDLKYISPEMMVAVLNGQFNSLIENCVIVDCRYPYEYEGGHIKGAVNLPLEQDIEDFLLKNPIVSLDVQKRVIVIFHCEFSSERGPRMCRFVREKDRACNEYPNLHYPELYILKGGYKEFFPQYQAHCEPRDYRPMHHEDFKEDLRKFRQKSRTWAGEKSKRELYSRLKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3A4</accession>
    <name>A0A452I3A4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">TGF-beta family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3A4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022000"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025065.1">
        <property type="protein sequence ID" value="ENSGAGP00000022000.1"/>
        <property type="gene ID" value="ENSGAGG00000016139.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19396">
        <property type="entry name" value="TGF_beta_BMP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000003">
        <property type="entry name" value="Bone morphogenetic protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000016">
        <property type="entry name" value="Bone morphogenetic protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF137">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0891">Chondrogenesis</keyword>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0339">Growth factor</keyword>
    <keyword evidence="6" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019045215" description="TGF-beta family profile domain-containing protein" evidence="13">
        <location>
            <begin position="20"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="14">
        <location>
            <begin position="358"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="81"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="337"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="477" mass="53761" checksum="BB2FFE59FC610510" modified="2019-05-08" version="1">MPLRLGWLWWGGLLCSCCCSPPPPVLPLLPAGGALLADSSREKPQAPAASSPGFLYRRLKSHEKREMQKEILSVLGLQHRPRPLHGLQPRPPPPQQQQQQQQQLPPPDRLNSAPLFMLDLYNALSSEEEQGPGAERRPLQRPTAHTLQRKTLIAQPGPSGGSPSASSQDSAFLNDADMVMSFVNLVEYDKEFTPHQRHHKEFKFNLSQIPEGEAVTAAEFRIYKDCVMGSFKNQTFLINIYQVLQEHQNRDSDLFLLDTRAVWASEEGWLEFDITATSNMWVMNPQHNMGLQLSVVTRDGLSVNPRAAGLIGRNGPYDKQPFMVAFFKVSEVHVRTTRSATSRRRQQNRNRSTQSQDVSRVSSITDYNSSDLKTACRKHELFVSFQDLGWQDWIIAPKGYAANYCDGECSFPLNAHMNATNHAIVQTLVHLMNPEYVPKPCCAPTKLNAISVLYFDDNSNVILKKYRNMVVRACGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3G5</accession>
    <name>A0A452I3G5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3G5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022068"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025153.1">
        <property type="protein sequence ID" value="ENSGAGP00000022068.1"/>
        <property type="gene ID" value="ENSGAGG00000016204.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043542">
        <property type="term" value="P:endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09336">
        <property type="entry name" value="LIM1_Paxillin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09407">
        <property type="entry name" value="LIM2_Paxillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000008">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000018">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047072">
        <property type="entry name" value="Paxillin_Lim_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047075">
        <property type="entry name" value="Paxillin_TGFB1I1_LIM_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF11">
        <property type="entry name" value="PAXILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03535">
        <property type="entry name" value="Paxillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00832">
        <property type="entry name" value="PAXILLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 10" id="KW-0440">LIM domain</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="12">
        <location>
            <begin position="943"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="12">
        <location>
            <begin position="1003"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="18"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="77"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="156"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="390"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="536"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="754"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="889"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="80"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="428"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="545"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="585"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="754"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="766"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1132" mass="123594" checksum="3B37647C2CD706B7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4X0</accession>
    <name>A0A452I4X0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I4X0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022616"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025763.1">
        <property type="protein sequence ID" value="ENSGAGP00000022616.1"/>
        <property type="gene ID" value="ENSGAGG00000016582.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051860">
        <property type="entry name" value="Plasmodium_CSP_Invasion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44826">
        <property type="entry name" value="SPORE COAT PROTEIN SP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44826:SF3">
        <property type="entry name" value="SPORE COAT PROTEIN SP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="420" mass="46770" checksum="E368AE9A4BEE95F9" modified="2019-05-08" version="1">MRQHGAGLIMVLTKSTRMTPTRKHQRRPSAPTLSCCSISRGLLFHGEHHSSTFPWSSPSIRPSIELTIHPPLHGDHHPSSLPQRSPSICPSMELTPHLSFHGDHHPPALPQRSPSILPSTEITTHPPFHGDHHPSSLPQTSPSIRPSMELTTHPSFHRDHHPSFLPRRSPSIRPSTELTTHPPLHGDYHPSSLPQRSPSICPSTEITIHPPLHGAHHPSVLPRRSSSIHHSMEITIHLPFHRDHHPSSLPLRSPPIRPSTEITIHPPFHRAHHPPAPPWSSPPIRPSMEITIHPPLHGDHHPPSLPQRSSSILPSTEITIHLPLHGAHPPSVLPRRSPSILPSTEITIHPPVHGDYYFVQSLLRMLVREVCFQSSLQFLVWAIFLWNLPTGSLEGLPPDLRSSGRSPGFLARCPTPDPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5V5</accession>
    <name>A0A452I5V5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I5V5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022916"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026116.1">
        <property type="protein sequence ID" value="ENSGAGP00000022916.1"/>
        <property type="gene ID" value="ENSGAGG00000016804.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11816">
        <property type="entry name" value="SH3_Eve1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11817">
        <property type="entry name" value="SH3_Eve1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11818">
        <property type="entry name" value="SH3_Eve1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035835">
        <property type="entry name" value="Eve1_SH3_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF48">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="409"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="483"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="559"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="635"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="699"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="219"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="330"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="756" mass="83225" checksum="B4AB216D81CDB303" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I674</accession>
    <name>A0A452I674_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein 100</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I674"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023082"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026300.1">
        <property type="protein sequence ID" value="ENSGAGP00000023082.1"/>
        <property type="gene ID" value="ENSGAGG00000016912.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071773">
        <property type="term" value="P:cellular response to BMP stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032536">
        <property type="entry name" value="TMEM100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100">
        <property type="entry name" value="PHOSPHOINOSITIDE-INTERACTING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16311">
        <property type="entry name" value="TMEM100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="13456" checksum="57018B8276569908" modified="2019-05-08" version="1">TNPSIHPSPLGEPRSPVNMATEEVQIQLCPDPRLVSTATGGVEGACHSCLLPFSVVAALIGVAVTALAYARDAQGLVLGVLGLSLLGAGVLGLAGSYMTYRCRGRKIRGWRRGSFTLLVGDQQQKKAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I803</accession>
    <name>A0A452I803_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I803"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023767"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027064.1">
        <property type="protein sequence ID" value="ENSGAGP00000023764.1"/>
        <property type="gene ID" value="ENSGAGG00000017392.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000027070.1">
        <property type="protein sequence ID" value="ENSGAGP00000023767.1"/>
        <property type="gene ID" value="ENSGAGG00000017392.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000027075.1">
        <property type="protein sequence ID" value="ENSGAGP00000023772.1"/>
        <property type="gene ID" value="ENSGAGG00000017392.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000027083.1">
        <property type="protein sequence ID" value="ENSGAGP00000023777.1"/>
        <property type="gene ID" value="ENSGAGG00000017392.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006017">
        <property type="entry name" value="Caldesmon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006018">
        <property type="entry name" value="Caldesmon_LSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18949">
        <property type="entry name" value="CALDESMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18949:SF0">
        <property type="entry name" value="CALDESMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02029">
        <property type="entry name" value="Caldesmon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01076">
        <property type="entry name" value="CALDESMON"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="755"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="785"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="577"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="601"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="789"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="833"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="880" mass="103133" checksum="D4AC898A71D2E2F2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IC19</accession>
    <name>A0A452IC19_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000025311"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028813.1">
        <property type="protein sequence ID" value="ENSGAGP00000025311.1"/>
        <property type="gene ID" value="ENSGAGG00000018517.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF15">
        <property type="entry name" value="CERAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="8">
        <location>
            <begin position="34"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="289" mass="32178" checksum="AC9161DE01A630A2" modified="2019-05-08" version="1">MFHALVAGGIVFPGLFLLAKNSLKRLPWLQWDEADAVIISARLVSSVQAVMASTAGYIVFSSCQHVLDDQHWLTSAYTQFAVPYFIYDIYAMFLCHWHKYRVKGHEGAGPRSLRTVTCAFLHKDFLMVLHHLCMVLVCFPVSVLWRQGKGDFFLGCMLMAELSTPFVCLGKVLILYKRQHTVLHKVNGLLMLVTFFCCRILLFPYMYWAYGRHAGLPLHRVPLALPPACNLGAALLLAPQLYWFGLICRGAARLFRRARPKDTTAAALRNGHGPGGAGTGLSPCPADGX</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IE36</accession>
    <name>A0A452IE36_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IE36"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026155"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029745.1">
        <property type="protein sequence ID" value="ENSGAGP00000026155.1"/>
        <property type="gene ID" value="ENSGAGG00000019100.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15947">
        <property type="entry name" value="7tmA_OR2B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000005">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="42"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="327" mass="36378" checksum="C12773E4618398CA" modified="2019-05-08" version="1">MWEDGNQSSLGEFILLGVSDWPQFELLLFVLILISYTMTLLGNTTIILVSSLDPHLHTPMYFFLSNLSFLDLCYTTSVGPQMLLNFRSTHKSISWAGCVAQLYIALSLGSTECLMLAIMAYDRYASICQPLRYTAIMSHRFCLQLAATAWLCSFGNSMLHTIRTMRLPRCGRNQIDSLFCEVPALLKLACVDTSANEAEVLASSVIILLVPLGFILMSYGYIGAAVLKISSAKGRIKAFNTCASHLAVVLLFYGTAISMYLQPPSSYSQHRGKIVSLFYTMVTPMLNPIIYTLRNKEVHGALQRKVLGRSPTTQWAQTLKLWLVAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IEZ8</accession>
    <name>A0A452IEZ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sestrin 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a hydroperoxide + L-cysteinyl-[protein] = S-hydroxy-L-cysteinyl-[protein] + an alcohol</text>
            <dbReference type="Rhea" id="RHEA:67124"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:17193"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:61973"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:67125"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sestrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IEZ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026404"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030016.1">
        <property type="protein sequence ID" value="ENSGAGP00000026404.1"/>
        <property type="gene ID" value="ENSGAGG00000019248.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070728">
        <property type="term" value="F:L-leucine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016684">
        <property type="term" value="F:oxidoreductase activity, acting on peroxide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071233">
        <property type="term" value="P:cellular response to L-leucine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990253">
        <property type="term" value="P:cellular response to leucine starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901031">
        <property type="term" value="P:regulation of response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1290.10:FF:000001">
        <property type="entry name" value="Sestrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006730">
        <property type="entry name" value="Sestrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12474">
        <property type="entry name" value="P53 REGULATED PA26 NUCLEAR PROTEIN SESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12474:SF1">
        <property type="entry name" value="SESTRIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04636">
        <property type="entry name" value="PA26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="52297" checksum="CB37BEF62088973C" modified="2019-05-08" version="1">MILCPPSTEHPRGNQCQRLQGQVVKMSGSDQERPQFLFVKALASRGRLEAVTQQMGYHPQYLDSFLKMQHYLLHMDGPLPFACRHYIAIMAAARHQCWYLVNLHMLQFLRVGGDPQWLRGLDFIPPKLRNLNEINKILAHRPWLISKEHIEKLLKISEQSWSLAELVHAVVLVAHYHALASFVFGCGCEQEWLDGRSILKSTLPGNQCFCESASGNSCNLELLHINRKRSLDSCVELESLRERIQRIHLETEGREETRPPPQDREEDFDGEIVRATDLSCYVQDPNFGYQDFARRDEEQTQIFRVQDYSWEDHGFSLVNRLYSDVGHLLDEKFRTVDSLQSNAMARRQGCESSVFKRGIWNYIHCMFGIRYDDYDYAEVNHFLERMLKVYIKTVTCYPEKTNPEMFSRFWKQFKHGEKVHVNLLILEARMQAELLYALQAITQYMIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IG81</accession>
    <name>A0A452IG81_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">E3 ubiquitin-protein ligase RNF14</fullName>
            <ecNumber evidence="5">2.3.2.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">RING finger protein 14</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with GCN1; interaction takes place in response to ribosome collisions and is required for ubiquitination of EEF1A1/eEF1A. Interacts with the ubiquitin-conjugating enzymes UBE2E1 and UBE2E2. Interacts with AR/androgen receptor. Interacts with TCF7/TCF1, TCF7L1/TCF3 and TCF7L2/TCF4; promoting Wnt signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the RBR family. RNF14 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452IG81"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026879"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030555.1">
        <property type="protein sequence ID" value="ENSGAGP00000026879.1"/>
        <property type="gene ID" value="ENSGAGG00000019573.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060828">
        <property type="term" value="P:regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20341">
        <property type="entry name" value="BRcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20354">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16628">
        <property type="entry name" value="RING-HC_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23820">
        <property type="entry name" value="RWD_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000011">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000007">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000049">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000186">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047548">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031128">
        <property type="entry name" value="RNF14_RING-HC_Zfn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RWD" evidence="24">
        <location>
            <begin position="11"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="25">
        <location>
            <begin position="216"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="23">
        <location>
            <begin position="220"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00067098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00075528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="474" mass="53906" checksum="031AD0CB974E10E4" modified="2019-05-08" version="1">MSSEDKEAQEDELLALASIYDEDEFKRADSAQGGETRICLELPQNFKIFMSGSPTECLQNRGFEYTICFLPPLVLNFELPPDYPSVSPPVFTLSSKWLSPTQLTALCKHLDNLWEENRGCVVLFAWMQFLKEETLAYLNITSPYELKLCHQENRQSRTSLFPLNAELDDCGAAGAATAQEEILDERAVQDVESLSSLIGEILDFDQAQQKKCFNSKMFLCSICFSEKLGSECMYFMECRHIYCKSCLKDYFEIQIRDGQVHCLSCPEPKCSSVATPGQVKELVGEELFARYDRLLLQSSLDLMADVVYCPRPCCQTPVMQEPGCTMGICSSCNYAFCTVCKMTYHGVSPCKVTAEKLMALRNEYQEADEATKIFLEQRYGKRVIQKALEEVESRKWLEKNSKACPCCGTPIEKLDGCNKMTCTGCRQYFCWLCMGSLSRVNPYRHFNDTSSPCFNRLFQAMNVDGDFWDAENED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHV1</accession>
    <name>A0A452IHV1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IHV1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027284"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030992.1">
        <property type="protein sequence ID" value="ENSGAGP00000027284.1"/>
        <property type="gene ID" value="ENSGAGG00000019839.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050290">
        <property type="term" value="F:sphingomyelin phosphodiesterase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006685">
        <property type="term" value="P:sphingomyelin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024129">
        <property type="entry name" value="Sphingomy_SMPD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12988">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12988:SF6">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14724">
        <property type="entry name" value="mit_SMPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="775"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="807" mass="92434" checksum="9A934709413D70D6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJ31</accession>
    <name>A0A452IJ31_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJ31"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027894"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031683.1">
        <property type="protein sequence ID" value="ENSGAGP00000027894.1"/>
        <property type="gene ID" value="ENSGAGG00000020228.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000316">
        <property type="entry name" value="Neogenin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000101">
        <property type="entry name" value="Neogenin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000106">
        <property type="entry name" value="Neogenin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000133">
        <property type="entry name" value="Neogenin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000216">
        <property type="entry name" value="neogenin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010560">
        <property type="entry name" value="Neogenin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF14">
        <property type="entry name" value="NEOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06583">
        <property type="entry name" value="Neogenin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="692"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="36"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="133"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="228"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="332"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="447"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="548"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="29"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="654"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="726"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="762"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="875"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="675"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="779"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="887"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1045" mass="114146" checksum="8D558B08BC6F17B1" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKG4</accession>
    <name>A0A452IKG4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Bifunctional apoptosis regulator</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Membrane-bound E3 ubiquitin ligase that plays a role in several processes including apoptosis regulation or reticulum endoplasmic stress. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors. Contributes to the dynamic control of IRE1/ERN1 signaling during ER stress by inducing BAX inhibitor 1/TMBIM6 proteasomal degradation. Promotes the activation of TGF-beta signaling by mediating the 'Lys-63'-linked ubiquitination of TGFBR1 which is critical to activate the pathway. Together with NGFR, negatively regulates NF-kappa-B and JNK-related signaling pathways. Promotes the proteasome-mediated degradation of PNPLA3, a protein involveld in lipid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with CASP8, BCL2 and BCL2L1 through SAM domain and also with HIP1, IFT57, ESRRBL1 and BCAP31. Interacts with NGFR; this interaction inhibits NF-kappa-B and JNK-related signaling pathways.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452IKG4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028460"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032327.1">
        <property type="protein sequence ID" value="ENSGAGP00000028460.1"/>
        <property type="gene ID" value="ENSGAGG00000020630.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16497">
        <property type="entry name" value="RING-HC_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09513">
        <property type="entry name" value="SAM_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000053">
        <property type="entry name" value="Bifunctional apoptosis regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000331">
        <property type="entry name" value="Bifunctional apoptosis regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15898">
        <property type="entry name" value="BIFUNCTIONAL APOPTOSIS REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15898:SF13">
        <property type="entry name" value="BIFUNCTIONAL APOPTOSIS REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="404"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="40"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="21">
        <location>
            <begin position="187"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00055004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="452" mass="53463" checksum="A6C9C44E7D47B345" modified="2019-05-08" version="1">MHKGKMEEDVKLQRENEKLKVKADASPEISRLISVSEFSCHCCYDVLVNPTTLNCGHSFCRHCLALWWVSSKKNECPECREKWEGFPKVNILLRDAIEKLFPDDIEQRKEDIQQNSDVAHSLEAFQKHGSDQTYAPPNVRRINPRGGGFFSGVLTALTCVAVVLLGYHWSSREYEDDLLVHKPVAKWTPEEVILWLEQLGPWASLYRERFLLVNGRLLLTITDEDFTKTPYHIENSSHRKAIIMELERVKTLGVKPPQNLWEYKAVNPGKSLFLLYALKSSPRLSMLYLYLFDYTEIFLPFIYTVCPVQEDEYEDTITKLLDLKDPTWKQWREFIVKYSFLPYQLIAEFAWDWLEIHYWTSRFIIVNAMLLSVLELFSLWRLWSRRELKTIPQRMWSHFWKVSTQGLFVAVFWPIIPQFVCNCLFYWALYFNPIINIDLVVKEVRRLETQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKH8</accession>
    <name>A0A452IKH8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IKH8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028475"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032343.1">
        <property type="protein sequence ID" value="ENSGAGP00000028475.1"/>
        <property type="gene ID" value="ENSGAGG00000020637.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051308">
        <property type="term" value="P:male meiosis chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007127">
        <property type="term" value="P:meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033587">
        <property type="entry name" value="M1AP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28642">
        <property type="entry name" value="MEIOSIS 1 ARREST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28642:SF1">
        <property type="entry name" value="MEIOSIS 1 ARREST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="452" mass="50802" checksum="18C2BE1A9810AD8B" modified="2019-05-08" version="1">LHPYISKGYTAGKAYATAHSWQLPQILIVDVKPPFWANTCSKLCEALENFFCLACSLAGPCRIPLLSLYVVQNQHECLLPFVQVKGSFPRLQTCISELRSLPREGSFQQKGDRVMQAVQDGLQQFKQYTRHMLVATALDLCLLFQITVLTSQPGKDMVKQLETGLREIDLVSLRKLQIIEISQGDLLESADVEWHIHAEEEVSNSAILGMDIDLQIIENDVVSLETFFKTWLHDHGTDREQLHLLLPSGDFRHFTAPRTSLTCVKCDIQERLLSPALLPGAADVTVKTDDVNTPYQMAASPATTLYKLRVIKALKAEGVCESALFGLPFIIKPTSCWQLDWDELEMNQHNFHALCHSLLKRDWMLLAKREPQSVSPSWRITVNSYYVIIPSDSFTLLVKAIAVRELLLPCNIPALPADLPETALSTIEASDEWLHTSLDKEAVQVLQVQDTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKR4</accession>
    <name>A0A452IKR4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000028422"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032283.1">
        <property type="protein sequence ID" value="ENSGAGP00000028422.1"/>
        <property type="gene ID" value="ENSGAGG00000020606.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000032288.1">
        <property type="protein sequence ID" value="ENSGAGP00000028425.1"/>
        <property type="gene ID" value="ENSGAGG00000020606.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000032292.1">
        <property type="protein sequence ID" value="ENSGAGP00000028429.1"/>
        <property type="gene ID" value="ENSGAGG00000020606.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000019">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000031">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000036">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000048">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000068">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR GLI3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="482"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="520"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="548"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="578"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="609"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="373"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="622"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="865"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1112"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="634"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="660"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="686"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="702"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="865"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1129"/>
            <end position="1149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1575" mass="171971" checksum="7E91748051198C8E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILM8</accession>
    <name>A0A452ILM8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the repulsive guidance molecule (RGM) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILM8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028753"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032666.1">
        <property type="protein sequence ID" value="ENSGAGP00000028753.1"/>
        <property type="gene ID" value="ENSGAGG00000020841.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1000.10:FF:000001">
        <property type="entry name" value="Repulsive guidance molecule BMP co-receptor a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040287">
        <property type="entry name" value="RGM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009496">
        <property type="entry name" value="RGM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010536">
        <property type="entry name" value="RGM_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428:SF5">
        <property type="entry name" value="REPULSIVE GUIDANCE MOLECULE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428">
        <property type="entry name" value="RGM DOMAIN FAMILY MEMBER DRAG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06534">
        <property type="entry name" value="RGM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06535">
        <property type="entry name" value="RGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Repulsive guidance molecule N-terminal" evidence="13">
        <location>
            <begin position="83"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Repulsive guidance molecule C-terminal" evidence="12">
        <location>
            <begin position="189"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="466" mass="51254" checksum="AFA0C58673DE17EB" modified="2019-05-08" version="1">MMRKRRKPGGRLACSCSSPVPPPPESRRPAWTGMGRAAPSCAAAGAEQRHRRLHQPPPPAALFLLFNLWLLLRTGDCQLQTPCRIQKCTTDFVSLTSHLNSALDGFDLEFCKALRAYSACTYRNSKVCRGNLVYHSAVLGISDLMSQRNCSKDGPTSSTNPEVTHDPCNYSGRIGARELRGGEQTPPTYLFCGLFGDPHLRTFKDHFQTCKVEGAWPLIDNNYLSVQVTNVPLVPGSSATATNKITIIFKSYQDCTEQKVYQAVTDDLPAAFVDGTTSGGDSDTKSLRIVERVSGRSVEMHARYIGATVFVRQVGRYLTLAIRMPEELAMAYEESQDLQLCVNGCPSSERIDDSGHLPLPVMGQLLPHAASTQPWSVYTLESATTKCHEKMLVKDIYFYSCVFDLLTTGDANFTAAAHSALQDVEALHPRKERWHIFPNSGNGDMGKNSKLAVSLGLVCLILLAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMZ1</accession>
    <name>A0A452IMZ1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IMZ1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029321"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033300.1">
        <property type="protein sequence ID" value="ENSGAGP00000029321.1"/>
        <property type="gene ID" value="ENSGAGG00000021213.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="418" mass="47086" checksum="9E925DE8D5A9E954" modified="2019-05-08" version="1">MIVVTGIMSAPELGSTATTPVAAPNFSWMSDDGSPTAMEQPIFLMTTAAQAISGFFVWTALLITCHQIYMHLRCYSCPNEQRYIVRILFIVPIYAFDSWLSLLFFTNDQYYVYFGTVRDCYEAFVIYNFLSLCYEYLGGESSIMSEIRGKSIESSCMYGTCCLWGKTYSIGFLRFCKQATLQFCVVKPLMAISTVILQAFGKYRDGDFDVTRGYLYVTIIYNISVSLALYALFLFYFATRELLSPYSPVLKFFMVKSVIFLSFWQGMLLAILEKCGAIPKIHSANVSVGEGTVAAGYQDFIICVEMFFAALALRHAFTYKVYADKHLDAQAVPSYGPYGRCAPMKSISSSLKETMNPHDIVQDAIHNFSPAYQQYTQQSTLEHGPPWRNGSHIISRSHSCSGARDNEKTLLLSSDDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPE9</accession>
    <name>A0A452IPE9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IPE9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029861"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033905.1">
        <property type="protein sequence ID" value="ENSGAGP00000029861.1"/>
        <property type="gene ID" value="ENSGAGG00000021552.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="364" mass="40400" checksum="9DEBC56389BD96CA" modified="2019-05-08" version="1">MAAVGSPAGVTPGAEGAAEPDGSAGATGARPKGGRVVKARYLQYDRKPVGKSISANSSVVSGVKRSEKGETPTGRAGLFQKCKATTGTATSTLNRTVLGKDDLQSTLLEGHKIARPDLDLSAINDKSLLKKIPGSKSLFRAESRTRKKEQNSKSSDPDDVIGMLESQALLLNYASMKIEKNIAQLEEKGERNLSILCEEKEKQQKKLYELKRQLLLKKREQKLEEVLDKQVEVLTPLLTVCEQFKEEYKTFAAALDTTRHELPVKNIHIEGNRHTHLEDLKKHLAVTQSLLVEAAPGYLEENSKAFPVLKELKEVALKMDAELERSFKQVQDLSFEISKEVSLHNQKVCEDTHGIETLQHWYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPU4</accession>
    <name>A0A452IPU4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and might also direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IPU4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029970"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034037.1">
        <property type="protein sequence ID" value="ENSGAGP00000029970.1"/>
        <property type="gene ID" value="ENSGAGG00000021634.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21507">
        <property type="entry name" value="PPP2R3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.220:FF:000001">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000628">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.230:FF:000001">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041534">
        <property type="entry name" value="EF-hand_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048855">
        <property type="entry name" value="P2R3A_B_D_EF-hand"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14095">
        <property type="entry name" value="PHOSPHATASE 2A REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14095:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17958">
        <property type="entry name" value="EF-hand_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21161">
        <property type="entry name" value="P2R3B_EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="397"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="82"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00093310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="582" mass="67428" checksum="CBF6CED2452ADE27" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPV3</accession>
    <name>A0A452IPV3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nck-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Lamellipodium membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the HEM-1/HEM-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IPV3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029939"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033998.1">
        <property type="protein sequence ID" value="ENSGAGP00000029939.1"/>
        <property type="gene ID" value="ENSGAGG00000019065.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031258">
        <property type="term" value="C:lamellipodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019137">
        <property type="entry name" value="Nck-associated_protein-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12093">
        <property type="entry name" value="NCK-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12093:SF11">
        <property type="entry name" value="NCK-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09735">
        <property type="entry name" value="Nckap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="530"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="541"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1018" mass="116231" checksum="00420911DB37003A" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQ11</accession>
    <name>A0A452IQ11_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM9 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IQ11"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029999"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034068.1">
        <property type="protein sequence ID" value="ENSGAGP00000029999.1"/>
        <property type="gene ID" value="ENSGAGG00000021656.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000034069.1">
        <property type="protein sequence ID" value="ENSGAGP00000030000.1"/>
        <property type="gene ID" value="ENSGAGG00000021656.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008853">
        <property type="entry name" value="TMEM9/TMEM9B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13064:SF1">
        <property type="entry name" value="PROTON-TRANSPORTING V-TYPE ATPASE COMPLEX ASSEMBLY REGULATOR TMEM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13064">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05434">
        <property type="entry name" value="Tmemb_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604585" evidence="8">
        <location>
            <begin position="48"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="209" mass="23684" checksum="2307C8B3BB846065" modified="2019-05-08" version="1">MGILPPTHSFIFLFQAPGMLTQHSWENMKILWLAAVAWCVLAPPAQASKSSEDIRCKCICPPYRNISGHIYNKNVSQKDCNCLHVVEPMPVPGNDVEAYCLLCECKYEERSTTTIKIIIIIYLSVVGALLLYMAFLMLVDPLIRKPDAYTQPLHNEEENEEARSLAAAPAPTGARANTVLERVEGAQQRWKRQVQEQRKTVFDRHKMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQ64</accession>
    <name>A0A452IQ64_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ciliary neurotrophic factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CNTF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IQ64"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030050"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034121.1">
        <property type="protein sequence ID" value="ENSGAGP00000030050.1"/>
        <property type="gene ID" value="ENSGAGG00000021682.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005127">
        <property type="term" value="F:ciliary neurotrophic factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048143">
        <property type="term" value="P:astrocyte activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070120">
        <property type="term" value="P:ciliary neurotrophic factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048680">
        <property type="term" value="P:positive regulation of axon regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.10:FF:000022">
        <property type="entry name" value="ciliary neurotrophic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000151">
        <property type="entry name" value="Ciliary_neurotrophic_fac_CNTF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15196">
        <property type="entry name" value="CILIARY NEUROTROPHIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15196:SF0">
        <property type="entry name" value="CILIARY NEUROTROPHIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01110">
        <property type="entry name" value="CNTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="196" mass="21333" checksum="56CD49E8D3BCEEA2" modified="2019-05-08" version="1">MASAEQSPVPLQHRSLCSRVILLARKIHSDVASLLESYVERQGLDRTISLDSVDGVPAAAMEQGGELTVAERLDANLQAYRAFQVLLGEVLEEQRSHLTPLDTDFHASIQSVLLQVVALACQLGELLALLGHGRPAWEAASPEDPGHRSLFEMKLQGLKVLQELAHWTVRSVRDLRQVAKHSQGSGAAHGGQAQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRW7</accession>
    <name>A0A452IRW7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein ABHD14A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Alpha/beta hydrolase domain-containing protein 14A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Possible role in granule neuron development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the AB hydrolase superfamily. ABHD14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IRW7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030759"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034886.1">
        <property type="protein sequence ID" value="ENSGAGP00000030759.1"/>
        <property type="gene ID" value="ENSGAGG00000022119.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000093">
        <property type="entry name" value="protein ABHD14A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46197:SF1">
        <property type="entry name" value="PROTEIN ABHD14A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46197">
        <property type="entry name" value="PROTEIN ABHD14B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="15">
        <location>
            <begin position="97"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="271" mass="29746" checksum="CF323E366413FDFE" modified="2019-05-08" version="1">MLPERLTMALIRTRLGLLVLGVLVTFVLYLLLPAIQHERFTSRAGSHKAQARAEEKGQRDVNVTVLTGTIAGSPSILFREGFLLQRDGTPSPKRLEVIFLHGQAFTSKTWEDLGTLTLLSEEGYRSIAIDLPGYGNSLPSDAVSTEQGRVAFLQHVLKELGIQRPVLISPSMSGRYSIPFVLVHGAQLKGFVPIAPVGTQDYTSQQYQQVQTPTLIVYGERDTGLGAQSLQSLQQLPKHKAVALPDAGHACYLEKPHEFHEALLAFLGELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IS84</accession>
    <name>A0A452IS84_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IS84"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030788"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034917.1">
        <property type="protein sequence ID" value="ENSGAGP00000030788.1"/>
        <property type="gene ID" value="ENSGAGG00000021787.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032835">
        <property type="term" value="P:glomerulus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11827">
        <property type="entry name" value="SH3_MyoIe_If_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000004">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000007">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000010">
        <property type="entry name" value="Unconventional myosin-Ie"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035507">
        <property type="entry name" value="Ie/If_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF341">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 11" id="KW-0518">Myosin</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="14">
        <location>
            <begin position="1"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="15">
        <location>
            <begin position="490"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="812"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="11">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="678"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="779"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="693"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="796"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="869" mass="100138" checksum="ED4B2E2F31BA0834" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IT42</accession>
    <name>A0A452IT42_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IT42"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031046"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035198.1">
        <property type="protein sequence ID" value="ENSGAGP00000031046.1"/>
        <property type="gene ID" value="ENSGAGG00000022298.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021987">
        <property type="term" value="P:cerebral cortex development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18524">
        <property type="entry name" value="BACK_BTBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18348">
        <property type="entry name" value="BTB_POZ_BTBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000003">
        <property type="entry name" value="BTB/POZ domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.820:FF:000001">
        <property type="entry name" value="BTB/POZ domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000015">
        <property type="entry name" value="BTB/POZ domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.820">
        <property type="entry name" value="PHR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012983">
        <property type="entry name" value="PHR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038648">
        <property type="entry name" value="PHR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774:SF2">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08005">
        <property type="entry name" value="PHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="126"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="528" mass="58938" checksum="54601B50963463A1" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUA6</accession>
    <name>A0A452IUA6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">26S proteasome non-ATPase regulatory subunit 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1.</text>
    </comment>
    <comment type="function">
        <text evidence="1 8">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases and few additional components including PSMD2. Interacts with RPGRIP1L. Interacts with CRY1 in a KDM8-dependent manner. Interacts (via C-terminus) with phosphatase UBLCP1 (via ubiquitin-like domain); the interaction recruits UBLCP1 to the 19S regulatory particle where it dephosphorylates 19S subunit PSMC2/RPT1 which impairs PSMC2 ATPase activity and disrupts 26S proteasome assembly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the proteasome subunit S2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IUA6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031584"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035819.1">
        <property type="protein sequence ID" value="ENSGAGP00000031584.1"/>
        <property type="gene ID" value="ENSGAGG00000022682.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000035822.1">
        <property type="protein sequence ID" value="ENSGAGP00000031586.1"/>
        <property type="gene ID" value="ENSGAGG00000022682.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000026">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016643">
        <property type="entry name" value="26S_Psome_Rpn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041433">
        <property type="entry name" value="RPN1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040892">
        <property type="entry name" value="RPN1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18051">
        <property type="entry name" value="RPN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17781">
        <property type="entry name" value="RPN1_RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015965">
        <property type="entry name" value="26S_Psome_Rpn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RPN1 N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit RPN1 C-terminal" evidence="11">
        <location>
            <begin position="796"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="566"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="566"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR015965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="851" mass="94077" checksum="398EB362E22FB53F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYC1</accession>
    <name>A0A452IYC1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Nonsense-mediated mRNA decay factor SMG8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SMG8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYC1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033051"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037471.1">
        <property type="protein sequence ID" value="ENSGAGP00000033051.1"/>
        <property type="gene ID" value="ENSGAGG00000023604.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019354">
        <property type="entry name" value="SMG8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13091">
        <property type="entry name" value="AMPLIFIED IN BREAST CANCER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13091:SF0">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY FACTOR SMG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10220">
        <property type="entry name" value="Smg8_Smg9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019177498" description="Nonsense-mediated mRNA decay factor SMG8" evidence="6">
        <location>
            <begin position="18"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="575"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="644"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="666"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="676"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="997" mass="110200" checksum="3B25A7FE6E08F3DA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYD1</accession>
    <name>A0A452IYD1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IYD1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033088"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037508.1">
        <property type="protein sequence ID" value="ENSGAGP00000033088.1"/>
        <property type="gene ID" value="ENSGAGG00000023620.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF277">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="112"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="245" mass="27637" checksum="C2BA0D639297A6B9" modified="2019-05-08" version="1">MRPSGEHGRAVPRRSPGFAFSPSRQGMESSPSEKGRQLTETWHPRCCCCGPKPCCFCWCCCSSCSRGWRGWVLKRERVVQLPGLCLEVTSLSLTLSSSPRLTLEEASSWKHSFDCVLASPAGRSIFVEFLRTEHSDENMAFWLACEELKREQSQEQVCEKAKMIYLDYISILSPKEVSIDATVRESINSSLARPSAHIFNEAQAQVYALMHRDSYPRFLASPLYRSLEQRLSALASPIASSREMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYJ2</accession>
    <name>A0A452IYJ2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCLAF1/THRAP3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYJ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033127"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037549.1">
        <property type="protein sequence ID" value="ENSGAGP00000033127.1"/>
        <property type="gene ID" value="ENSGAGG00000023647.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000037552.1">
        <property type="protein sequence ID" value="ENSGAGP00000033130.1"/>
        <property type="gene ID" value="ENSGAGG00000023647.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029199">
        <property type="entry name" value="THRAP3_BCLAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15268:SF4">
        <property type="entry name" value="BCL-2-ASSOCIATED TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15268">
        <property type="entry name" value="THRAP3/BCLAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15440">
        <property type="entry name" value="THRAP3_BCLAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="469"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="673"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="865"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="223"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="397"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="446"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="472"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="673"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="751"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="764"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="816"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="898"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="909"/>
            <end position="921"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="928" mass="106610" checksum="619F5F9F2BA418A4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J2S4</accession>
    <name>A0A452J2S4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">M-phase inducer phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the MPI phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452J2S4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034706"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039287.1">
        <property type="protein sequence ID" value="ENSGAGP00000034706.1"/>
        <property type="gene ID" value="ENSGAGG00000024653.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01530">
        <property type="entry name" value="Cdc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000004">
        <property type="entry name" value="M-phase inducer phosphatase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000751">
        <property type="entry name" value="MPI_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF64">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06617">
        <property type="entry name" value="M-inducer_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00716">
        <property type="entry name" value="MPIPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5 7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="8">
        <location>
            <begin position="365"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="514" mass="58542" checksum="B5624281BE715FFA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5W2</accession>
    <name>A0A452J5W2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J5W2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035874"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040613.1">
        <property type="protein sequence ID" value="ENSGAGP00000035874.1"/>
        <property type="gene ID" value="ENSGAGG00000025453.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000040614.1">
        <property type="protein sequence ID" value="ENSGAGP00000035875.1"/>
        <property type="gene ID" value="ENSGAGG00000025453.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF318">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="260"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="425"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="454"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="500"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="556"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="587"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="631"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="676"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="525"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="528"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="751" mass="83265" checksum="EBD49441554F22FA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHV1</accession>
    <name>A0A452GHV1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21 22">Vacuolar protein sorting-associated protein 11 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 22">Cytoplasmic vesicle</location>
            <location evidence="2 22">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 22">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Late endosome membrane</location>
            <topology evidence="22">Peripheral membrane protein</topology>
            <orientation evidence="22">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 22">Lysosome membrane</location>
            <topology evidence="3 22">Peripheral membrane protein</topology>
            <orientation evidence="3 22">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Cytoplasmic vesicle</location>
            <location evidence="22">Clathrin-coated vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the VPS11 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GHV1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001199"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001347.1">
        <property type="protein sequence ID" value="ENSGAGP00000001199.1"/>
        <property type="gene ID" value="ENSGAGG00000000489.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048284">
        <property type="term" value="P:organelle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16688">
        <property type="entry name" value="RING-H2_Vps11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000164">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 11 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000570">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 11 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000258">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 11 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057308">
        <property type="entry name" value="CHCR_PEP5_VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057307">
        <property type="entry name" value="PEP5_VPS11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016528">
        <property type="entry name" value="VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024763">
        <property type="entry name" value="VPS11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF24">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23341">
        <property type="entry name" value="PEP5_VPS11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23356">
        <property type="entry name" value="TPR_PEP5_VPS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12451">
        <property type="entry name" value="VPS11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007860">
        <property type="entry name" value="VPS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16 22" id="KW-0072">Autophagy</keyword>
    <keyword evidence="17 25" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="20 22" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11 22" id="KW-0967">Endosome</keyword>
    <keyword evidence="19 22" id="KW-0458">Lysosome</keyword>
    <keyword evidence="18 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14 22" id="KW-0653">Protein transport</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 22" id="KW-0813">Transport</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 23" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="CHCR" evidence="24">
        <location>
            <begin position="403"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="24">
        <location>
            <begin position="568"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="26">
        <location>
            <begin position="813"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="763"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00073716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRNR" id="PIRNR007860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="932" mass="106793" checksum="FFA7CCC8C96F6626" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJ12</accession>
    <name>A0A452GJ12_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GJ12"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001810.1">
        <property type="protein sequence ID" value="ENSGAGP00000001591.1"/>
        <property type="gene ID" value="ENSGAGG00000001278.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022227">
        <property type="entry name" value="DUF3754"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16095:SF10">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16095">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 143 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12576">
        <property type="entry name" value="DUF3754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="456" mass="51424" checksum="3335D6BF1D320D4E" modified="2019-05-08" version="1">MVPWRNLKSLPRPDSWERTQQRNRLALAKDMAAGRRMLGVAWAALGTGVPGPPCRGTASLASRMAEYRRMWKPVEPQGWTEQYRERFIPFSKGQLVSALLKEFHSSSDAEHASFLAFVARVDSSLLHHYHSLLGRLQALYDPINPDRDTLPEMALSDTERLAKERQVLAELEPVLDQANFNSLSEDALAYALIVHHPQDDVQVSVNLDQYEYIRFWALGQRVGVLPIKSTLGPRKGLFGTARTPAERRYFKRVLVAARPRNAHLVLKCFKDIPLEALEQLLPAVRIRTSIFYKTLLNVTLVVSGLALFVNVGMVVLSDLKIGTSFLLLCFGAFMTFRAWKVFGQRRNIHSLELAYMLYYRSTSNNSELLGALVLRAQEEHAKELILAHSFLRRQPAWPPHGPADPETVASLQEHVQSWLRLHSGLEVSFCANRACRHLLSLEGGHRAHPAVAPGSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJ56</accession>
    <name>A0A452GJ56_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Superoxide dismutase [Cu-Zn]</fullName>
            <ecNumber evidence="7">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="7">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the C-terminal section; belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452GJ56"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001706"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001941.1">
        <property type="protein sequence ID" value="ENSGAGP00000001706.1"/>
        <property type="gene ID" value="ENSGAGG00000001369.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006801">
        <property type="term" value="P:superoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000004">
        <property type="entry name" value="Copper chaperone for superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00332">
        <property type="entry name" value="SOD_CU_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="8">
        <location>
            <begin position="102"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="277" mass="29106" checksum="6DA96BEE145AAA60" modified="2019-05-08" version="1">MEATPGPGEAGGPSCRLEFAVQMNCQECVDAVQASLQGATGLRVLDIRLDSQSVLVETSMSAGKVKGLLESTGRRAVLKGMGSSEPQNLGAAVAMMSGSSLVQGVVRFLQVSPEKCLIEGTIDGLEPGLHGLHVHEFGDVTNSCDSCGDHFNPDGERHGGPQDAHRHLGDLGNVLAGSDGRADFQMEDTRLKVWDIIGRSLVVDSGEDDLGRGGHPLSKVCGNSGERLACGIIARSAGLFENPKQICTCDGVTLWEEREKPVAGPGRRMLGQPASHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJD8</accession>
    <name>A0A452GJD8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SAM-dependent MTase RsmB/NOP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GJD8"/>
    <dbReference type="SMR" id="A0A452GJD8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001793"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002042.1">
        <property type="protein sequence ID" value="ENSGAGP00000001793.1"/>
        <property type="gene ID" value="ENSGAGG00000001438.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009383">
        <property type="term" value="F:rRNA (cytosine-C5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1170:FF:000001">
        <property type="entry name" value="Ribosomal RNA methyltransferase Nop2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023273">
        <property type="entry name" value="RCMT_NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054728">
        <property type="entry name" value="RsmB-like_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF30">
        <property type="entry name" value="28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22458">
        <property type="entry name" value="RsmF-B_ferredox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02012">
        <property type="entry name" value="RCMTNOP2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="11">
        <location>
            <begin position="305"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="603"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="610"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="667"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="522"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="397"/>
            <end position="403"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="679" mass="75730" checksum="1251767AB4EB4427" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKS1</accession>
    <name>A0A452GKS1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Josephin-1</fullName>
            <ecNumber evidence="4">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Josephin domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Josephin domain-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Josephin-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Cleaves 'Lys-63'-linked poly-ubiquitin chains, and with lesser efficiency 'Lys-48'-linked poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with beta-actin/ACTB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452GKS1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002367"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002714.1">
        <property type="protein sequence ID" value="ENSGAGP00000002367.1"/>
        <property type="gene ID" value="ENSGAGG00000001926.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.40:FF:000003">
        <property type="entry name" value="josephin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040053">
        <property type="entry name" value="JOSD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006155">
        <property type="entry name" value="Josephin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13291">
        <property type="entry name" value="JOSEPHIN 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13291:SF1">
        <property type="entry name" value="JOSEPHIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02099">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01246">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50957">
        <property type="entry name" value="JOSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Josephin" evidence="21">
        <location>
            <begin position="23"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="active site" evidence="20">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="active site" evidence="20">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="active site" evidence="20">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="202" mass="23335" checksum="CDBCC1D552090C2A" modified="2019-05-08" version="1">MSCVPWKGDKTKLESPEPPQLAPLCIYHEKQRRELCALHALNNVFQDSNAFTRETLQEIFQRLSPNTMVTPHKKSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVNVIALSNVMGFIMNLPSSLCWGPLKLPLKRQHWICVREVGGTYYNLDSKLKMPEWIGGESELRKFLKHQLRGKNCELLLVVPEEVEAHQSWRADM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLK8</accession>
    <name>A0A452GLK8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Latent transforming growth factor beta binding protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the LTBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GLK8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002674"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003066.1">
        <property type="protein sequence ID" value="ENSGAGP00000002674.1"/>
        <property type="gene ID" value="ENSGAGG00000002074.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000003">
        <property type="entry name" value="fibrillin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000068">
        <property type="entry name" value="Latent transforming growth factor beta binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000014">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000001">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000002">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000302">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000317">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000096">
        <property type="entry name" value="Putative fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050751">
        <property type="entry name" value="ECM_structural_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034:SF46">
        <property type="entry name" value="LATENT-TRANSFORMING GROWTH FACTOR BETA-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00683">
        <property type="entry name" value="TB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="95"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="14">
        <location>
            <begin position="275"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="353"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="14">
        <location>
            <begin position="401"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="567"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="609"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="653"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="736"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="777"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="236"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="480"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="916" mass="96844" checksum="CF7952A87FD7D9D7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMB7</accession>
    <name>A0A452GMB7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Leucine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="2">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Aminoacyl-tRNA synthetase that catalyzes the specific attachment of leucine to its cognate tRNA (tRNA(Leu)). It performs tRNA aminoacylation in a two-step reaction: Leu is initially activated by ATP to form a leucyl-adenylate (Leu-AMP) intermediate; then the leucyl moiety is transferred to the acceptor 3' end of the tRNA to yield leucyl-tRNA. To improve the fidelity of catalytic reactions, it is also able to hydrolyze misactivated aminoacyl-adenylate intermediates (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-methionyl-tRNA(Leu) + H2O = tRNA(Leu) + L-methionine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:77535"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:18931"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:77536"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:11689"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452GMB7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002950"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003381.1">
        <property type="protein sequence ID" value="ENSGAGP00000002950.1"/>
        <property type="gene ID" value="ENSGAGG00000002353.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000003392.1">
        <property type="protein sequence ID" value="ENSGAGP00000002959.1"/>
        <property type="gene ID" value="ENSGAGG00000002353.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07959">
        <property type="entry name" value="Anticodon_Ia_Leu_AEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000326">
        <property type="entry name" value="Leucine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000001">
        <property type="entry name" value="Leucine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000084">
        <property type="entry name" value="Leucyl-tRNA synthetase b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054509">
        <property type="entry name" value="LARS1_ULD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055416">
        <property type="entry name" value="RBD_LARS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24810">
        <property type="entry name" value="RBD_LARS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22947">
        <property type="entry name" value="ULD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="14">
        <location>
            <begin position="19"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="14">
        <location>
            <begin position="188"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="15">
        <location>
            <begin position="794"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine--tRNA ligase RagD-binding" evidence="17">
        <location>
            <begin position="940"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine--tRNA ligase ubiquitin-like" evidence="16">
        <location>
            <begin position="1064"/>
            <end position="1175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF22947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF24810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1176" mass="134304" checksum="3D4C962B85AB728F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMK5</accession>
    <name>A0A452GMK5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Protein Hikeshi</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Acts as a specific nuclear import carrier for HSP70 proteins following heat-shock stress: acts by mediating the nucleoporin-dependent translocation of ATP-bound HSP70 proteins into the nucleus. HSP70 proteins import is required to protect cells from heat shock damages. Does not translocate ADP-bound HSP70 proteins into the nucleus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Forms an asymmetric homodimer; required for binding and nuclear import of HSP70 proteins. Interacts with ATP-bound HSP70 proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the OPI10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMK5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002916"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003336.1">
        <property type="protein sequence ID" value="ENSGAGP00000002916.1"/>
        <property type="gene ID" value="ENSGAGG00000002331.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048364">
        <property type="entry name" value="Hikeshi-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008493">
        <property type="entry name" value="Hikeshi-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031318">
        <property type="entry name" value="OPI10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12925">
        <property type="entry name" value="HIKESHI FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12925:SF0">
        <property type="entry name" value="PROTEIN HIKESHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21057">
        <property type="entry name" value="Hikeshi-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05603">
        <property type="entry name" value="Hikeshi-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Hikeshi-like N-terminal" evidence="4">
        <location>
            <begin position="6"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Hikeshi-like C-terminal" evidence="5">
        <location>
            <begin position="136"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="197" mass="21688" checksum="EEBB515B81C4C854" modified="2019-05-08" version="1">MFGCLVAGRLVQAVAQQVAQDKFVFDLPDYENINHVVVFMLGTIPFPDGMGGSVYFCFPDQSGMAVWQLLGFVTNEKPSAIFKISGLKSGKGSQHPFGAMNIPQTPSVAQIGISVELLEHLAQQTPVASAAVSSVDSFTQFTQKMLDSFYNFASSFAVTQAQMTPNPSEAFIPANVVLKWYENFQRRLAQNPFFWKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMM8</accession>
    <name>A0A452GMM8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMM8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002937"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003363.1">
        <property type="protein sequence ID" value="ENSGAGP00000002937.1"/>
        <property type="gene ID" value="ENSGAGG00000002344.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18203">
        <property type="entry name" value="BTB_POZ_ZBTB12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001450">
        <property type="entry name" value="zinc finger protein 774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF29">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="13">
        <location>
            <begin position="33"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="386"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="221"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="430" mass="47305" checksum="21E79BD0495AA9E2" modified="2019-05-08" version="1">MASGVELLRFQLPGHEAATLRNMNQLRSEERFCDVTIVAESLKFRGHKVILAACSPFLRDQFLLNPSSELQVSLMHSAKIVADLLLSCYTGALEFAVRDIVNYLTAASYLQMEHVVEKCRNALSQFIQPQIGLKEDAPPAKGGAPRAPAGPSAQERARHRLPAWPLKLELEELELKDEEEEEDYGDDDGVSDICIVKVESALEVAQRQKKQQAVAVAWAKEGSPPPGWAKEQQQEAQVNSTVAEGPALPEPAPQGVVKACYSLAEDAEGEGLLIFPGGTYAEEGGGRPGAGRGGPAGPHPLRSIRCAKCEEAFQGVEKLVFHMRAQHFIFMCPRCGKQFNHSSNLNRHMNVHRGVKSHSCAVCGKSFTQKSTLHDHMNLHSGQRPYRCSYCDVRFAHKPAIRRHLKEQHGKTTAENVLEASVSEINVLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMR2</accession>
    <name>A0A452GMR2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMR2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002973"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003406.1">
        <property type="protein sequence ID" value="ENSGAGP00000002973.1"/>
        <property type="gene ID" value="ENSGAGG00000002375.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001953">
        <property type="entry name" value="NHE-2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032103">
        <property type="entry name" value="NHE_CaM-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF89">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16644">
        <property type="entry name" value="NEXCaM_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01086">
        <property type="entry name" value="NAHEXCHNGR2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="11 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019144371" description="Sodium/hydrogen exchanger" evidence="15">
        <location>
            <begin position="42"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="483"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="511"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="16">
        <location>
            <begin position="93"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="16">
        <location>
            <begin position="383"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/hydrogen exchanger regulatory region" evidence="17">
        <location>
            <begin position="627"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="804"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="864" mass="97533" checksum="4AA18E18E7D4A993" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMT4</accession>
    <name>A0A452GMT4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2HC/C3H-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GMT4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003140"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003617.1">
        <property type="protein sequence ID" value="ENSGAGP00000003140.1"/>
        <property type="gene ID" value="ENSGAGG00000002539.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026319">
        <property type="entry name" value="ZC2HC1A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049899">
        <property type="entry name" value="Znf_C2HC_C3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555">
        <property type="entry name" value="C2H2 ZINC FINGER CGI-62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555:SF68">
        <property type="entry name" value="ZINC FINGER PROTEIN 474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13913">
        <property type="entry name" value="zf-C2HC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52027">
        <property type="entry name" value="ZF_C2HC_C3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="18"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="53"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="93" mass="10905" checksum="7FA4960E241A8F55" modified="2019-05-08" version="1">MLLYCNCCLQPPVMRRPPTVVCYICGREFGTKSIGIHEPQCLKKWHNENDMLPKHLRRPEPKKPEVRSLGGKLQTKQKELRGCKLEKNRFKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNI3</accession>
    <name>A0A452GNI3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GNI3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003298"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003801.1">
        <property type="protein sequence ID" value="ENSGAGP00000003298.1"/>
        <property type="gene ID" value="ENSGAGG00000002668.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040247">
        <property type="entry name" value="DUF5524"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31097:SF2">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31097">
        <property type="entry name" value="SI:DKEY-276J7.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17662">
        <property type="entry name" value="DUF5524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="320" mass="36812" checksum="644AF0D69AF082D9" modified="2019-05-08" version="1">MRNMSKEPSGPPNRYASCEWYYHVPLKRSEKTVNSEISIPPPSQIPGLSNLGEHHNEITFGSRRKWIKDTDSEYVKLAKQGGRPDLLKHFTPSTRKTSPVAYAVPDWYTHHSKPLTDDESKAQVSSMPDYMVHEEFKSDQLNGNYETKRGPFDFDMKSIWQRDAEDKENKEKKKENAAQVEVHVGNTKEVKLPAINPKYPNRIPPSSANKEFHGGNRLYFPPMPGQKNNEPVNFSKLISNGYRNDWIQQRNDWEKKIQQTSKNNEQPEETLPPMEAGLCASLERSISGNEEKVHTLSLRACMFLIFDFWFIVVYLKLGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVB2</accession>
    <name>A0A452GVB2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G protein-activated inward rectifier potassium channel 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Inward rectifier K(+) channel Kir3.1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Potassium channel, inwardly rectifying subfamily J member 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GVB2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006062"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007061.1">
        <property type="protein sequence ID" value="ENSGAGP00000006062.1"/>
        <property type="gene ID" value="ENSGAGG00000004922.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015467">
        <property type="term" value="F:G-protein activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000019">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000006">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003274">
        <property type="entry name" value="K_chnl_inward-rec_Kir3.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF99">
        <property type="entry name" value="G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01327">
        <property type="entry name" value="KIR31CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 16" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 16" id="KW-0813">Transport</keyword>
    <keyword evidence="7 16" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="18">
        <location>
            <begin position="30"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="19">
        <location>
            <begin position="177"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="490" mass="55971" checksum="262E850944A9720F" modified="2019-05-08" version="1">MSALRRHLWDEYQAVGSARGSARRKRQRFVDKNGRCNVQHGNLGGESSRYLSDLFTTLVDLKWRWNLLIFLLTYTVAWLVMASMWWGIAYLRGDLHRAHDQSYHPCVANVYNFPSAFLFFIETEATIGYGHRYITERCPEGIVLFLFQSLLGSIVDAFLIGCMFIKMSQPKKRAETLMFSRAAVISQRDGRLCLMFRVGNLRNSHMVSAQIRCKLIKSRQTPEGEFLPLDQCELDVGFGTGADQLFLVSPLTICHEINPKSPFFCLSKRSLRSEQFEIVVILEGIVETTGMTCQARTSYTEDEVLWGHRFLPVMSLEDGFFRVDYSQFHATFEVPTPPYSVKEQEESLSLPSPFNTPIGNNRNRREHICSLDCIDILEEKTNKFPSKLQKISSRKAGLQRRVMRMTSSNVEKACSTGDLLKIQQISSVPSNEDGDERIQLKAFRIKSESLTQSTGDLELQKKPQGMLTLGTRLEDNLPAKLRKMNSDRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVF5</accession>
    <name>A0A452GVF5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GVF5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006108"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007114.1">
        <property type="protein sequence ID" value="ENSGAGP00000006108.1"/>
        <property type="gene ID" value="ENSGAGG00000003932.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051893">
        <property type="term" value="P:regulation of focal adhesion assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032886">
        <property type="term" value="P:regulation of microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21240">
        <property type="entry name" value="CH_MACF1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000009">
        <property type="entry name" value="dystonin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000002">
        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000027">
        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000089">
        <property type="entry name" value="microtubule-actin cross-linking factor 1 isoform X9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049538">
        <property type="entry name" value="PCN-like_spectrin-like_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169:SF25">
        <property type="entry name" value="MICROTUBULE-ACTIN CROSS-LINKING FACTOR 1, ISOFORMS 1_2_3_4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21019">
        <property type="entry name" value="Spectrin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21020">
        <property type="entry name" value="Spectrin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="383"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="278"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="666"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="280"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="299"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1076" mass="121738" checksum="0326E4194CE5A78B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWS3</accession>
    <name>A0A452GWS3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GWS3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006554"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007626.1">
        <property type="protein sequence ID" value="ENSGAGP00000006554.1"/>
        <property type="gene ID" value="ENSGAGG00000005310.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021088">
        <property type="entry name" value="Osteocrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35353">
        <property type="entry name" value="OSTEOCRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35353:SF1">
        <property type="entry name" value="OSTEOCRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11037">
        <property type="entry name" value="Musclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019517174" evidence="1">
        <location>
            <begin position="26"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="133" mass="14524" checksum="80E466FB86DD5709" modified="2019-05-08" version="1">MLQVRFGVVHSVLALSLLRWHTGTAVLAEAAPQPFDSSIVLSMESHSASSEEKSATDLAAKLLLLDELVSLENNVIETKKKRSFPGFGSPLDRLSASSVDLKGKQRKVVELPKRRFGIPLDRIGTNRLTSTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GX58</accession>
    <name>A0A452GX58_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF4371 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GX58"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006695"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007773.1">
        <property type="protein sequence ID" value="ENSGAGP00000006695.1"/>
        <property type="gene ID" value="ENSGAGG00000005399.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="3">
        <location>
            <begin position="41"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="557"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="644" mass="73640" checksum="D95D8A81CB7E62CB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0W1</accession>
    <name>A0A452H0W1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H0W1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008215"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009458.1">
        <property type="protein sequence ID" value="ENSGAGP00000008215.1"/>
        <property type="gene ID" value="ENSGAGG00000006520.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="352" mass="37684" checksum="294B445453E07E09" modified="2019-05-08" version="1">MWGVSQVGDEGTKPDNQSSCFFPPAQKSLLKPSGSEHEHEHFPFGARDVVVCLDEELIQQLSVCQKDTVQPHSTDSGSNPAQQPPDKACTFLTSPDDHEHLLELESLAGGDSSCTSTDSGICLQDSSSGLSQFSGSESQDYKRQLPGSDDSGINLVRHSLCLTLSSSGRDYTSVDEGQDQRERESDCSPLARGLSSEAVEFRGYLKQTKGTVERQQDRAEGQLPTQSPGGTDVTLEMGCSEPALAKGYLKQASPGLTCGNADTVLSRESGFQGFISRLECNSSSLLNYGALSISVPSKSLPELPKAPFALGIFNTDLLGTLPLVSSLHSNERLCLEIDSLSLLGSECKDSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1B2</accession>
    <name>A0A452H1B2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Monoacylglycerol O-acyltransferase 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H1B2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008244"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009492.1">
        <property type="protein sequence ID" value="ENSGAGP00000008244.1"/>
        <property type="gene ID" value="ENSGAGG00000006538.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF78">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="29068" checksum="F08C4DCF9CD0CB78" modified="2019-05-08" version="1">GGLLHPGGPPTLWCLDTPIFPCPLVKTAPLDPSRNYLFGFHPHGVLVAGAFGNFCTEATGFGELFPGLTPHLLMLPFWFRLPLFRDYIMSGGLVSSDKASVSYLLGHEGGGQVAVIAVGGPPESLEARPGALTLQILSRKGFIKAALQHGAALVPVFSFGENELFHQVPNPRGSLVRAVQERLQKLMGLALPLFHARGVFQYSFGLLPFRRPIHTVVGAPILVPRTPCPSHQAVDRLHATYLERLIALFEEHKTKYGVPPEKHLNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H360</accession>
    <name>A0A452H360_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H360"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009052"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010390.1">
        <property type="protein sequence ID" value="ENSGAGP00000009052.1"/>
        <property type="gene ID" value="ENSGAGG00000007014.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043843">
        <property type="term" value="F:ADP-specific glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01938">
        <property type="entry name" value="ADPGK_ADPPFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007666">
        <property type="entry name" value="ADP_PFK/GK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21208">
        <property type="entry name" value="ADP-DEPENDENT GLUCOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21208:SF0">
        <property type="entry name" value="ADP-DEPENDENT GLUCOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04587">
        <property type="entry name" value="ADP_PFK_GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51255">
        <property type="entry name" value="ADPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="485" mass="53337" checksum="97D899D1873A6E79" modified="2019-05-08" version="1">MGQKHLSVKAMLDAFLEAACKILWNPSANNFNKPKHSNFLPKLFDVPPSLSFSVFPAHFFFLSSFSRVNACVDVIVSGVKLLQALGLDPEDGKDHAILNSRKDLKEAFIHFMQKGAAAERFFSDAETFDHIAQAASEYPGAQLYVGGNAALIGQKLATNPNLKILLCGPIGPKLHELLDDNVIVPPESMQETDEFHLILEYQAGEEWGKLRAPNANRFIFSHDLSNGAMNMLEVFVSSLDEFQPDLVVLSGLHMMEGQSREVREKRLLEAVTSISDIPTDIPIHLELASMTDQDLMSKIMNQQVFPLVNSVGLNEQELLFLTQSASGPHASLGSWNGVPDVGIVSDILFWILKKHGRTMDKASDLTRIHFHTLAYHILVTVDGYWGNQIAAVAAGARVAGTQACATETIDTDRVFLKAPLEFVTSHTEAPSKISLNPGDPVAQWHREGISFHFTPVLVCKDPIRTVGLGDAISAEGLLYSELYPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3J1</accession>
    <name>A0A452H3J1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000009129"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010483.1">
        <property type="protein sequence ID" value="ENSGAGP00000009129.1"/>
        <property type="gene ID" value="ENSGAGG00000007209.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="27"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="443" mass="49160" checksum="17E1EBFEAE84766C" modified="2019-05-08" version="1">MAPDVQLEKEQRMGFFQTILVALVFLPLLMVASHNFLQNFTAAVPRHWCYIPAGDNATTEVTGDLLKIYVPMDGNQEPDRCLRFSTPQWQLLAPNGTSTNATEPCLDGWAYDRSVFNSTIITEWNLVCGQRALKDFAQSMYMVGVLLGAPVLGGLADRLGRRALLLWSLLQMGVMGTSAAFAPNLAAYCAFRFFSGMRCRVSSYPLPPALEWIPSQFRAMVSTILACSLTLGQLLLAGLAYAIRDWRQLQLASSVPFFLFFLYSWWIPESAHWLIINHRPEKALRNLRRVAQINGEKPEGEGISLEVWGTGYTVGSSGQRWGGWVSSPRVLSPWLWRTRPVSYTHLDVXLALDLQRFEGISIFLVQLIFGAVDVPFRMVVAVVANRLGRRLTQAACLVLGGLFILASVPVPQGESWAFGQISIAFPVAQGTVSDRCRCPAMAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3Y7</accession>
    <name>A0A452H3Y7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DnaJ homolog subfamily C member 16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Endoplasmic reticulum DNA J domain-containing protein 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Plays an important role in regulating the size of autophagosomes during the formation process.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3Y7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009299"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010680.1">
        <property type="protein sequence ID" value="ENSGAGP00000009299.1"/>
        <property type="gene ID" value="ENSGAGG00000007353.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000010685.1">
        <property type="protein sequence ID" value="ENSGAGP00000009303.1"/>
        <property type="gene ID" value="ENSGAGG00000007353.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02963">
        <property type="entry name" value="TRX_DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052448">
        <property type="entry name" value="DnaJ_C16_autophagy_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043361">
        <property type="entry name" value="DNAJC16_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44303">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44303:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="14">
        <location>
            <begin position="77"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="15">
        <location>
            <begin position="175"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="608"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="609"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="827" mass="96204" checksum="2A42008E9FBC0B95" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H5B3</accession>
    <name>A0A452H5B3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PLAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H5B3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009849"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011312.1">
        <property type="protein sequence ID" value="ENSGAGP00000009849.1"/>
        <property type="gene ID" value="ENSGAGG00000007751.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01753">
        <property type="entry name" value="PLAT_LOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000002">
        <property type="entry name" value="Arachidonate 5-lipoxygenase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052970">
        <property type="entry name" value="Inner_ear_hair_cell_LOXHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042062">
        <property type="entry name" value="PLAT_LOX_verte"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901:SF3">
        <property type="entry name" value="LIPOXYGENASE HOMOLOGY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901">
        <property type="entry name" value="PROTEIN CBG12474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="2">
        <location>
            <begin position="2"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="137" mass="15632" checksum="A27315C6E2E94D1F" modified="2019-05-08" version="1">MAVYKVQVATGRDSLAGTFDTISITLVGSHGESPKHVLDGFGFDFQPGSVREYKVPSKRALGPILLIRLHKEPYSFFPENMWYCNTVRVTSPEGNTYCFPCYRWIQGYCTVELAESTGVQPWRSLVCRYRQRHDCRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6X8</accession>
    <name>A0A452H6X8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H6X8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010471"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012019.1">
        <property type="protein sequence ID" value="ENSGAGP00000010471.1"/>
        <property type="gene ID" value="ENSGAGG00000008191.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031085">
        <property type="term" value="C:BLOC-3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903232">
        <property type="term" value="P:melanosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043989">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026091">
        <property type="entry name" value="HPS4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14407:SF9">
        <property type="entry name" value="BLOC-3 COMPLEX MEMBER HPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14407">
        <property type="entry name" value="HERMANSKY-PUDLAK SYNDROME 4 PROTEIN LIGHT-EAR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19033">
        <property type="entry name" value="Intu_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCZ1/INTU/HSP4 first Longin" evidence="2">
        <location>
            <begin position="15"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="CCZ1/INTU/HPS4 third Longin" evidence="3">
        <location>
            <begin position="607"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="715" mass="77929" checksum="9306AA52117324E0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7H1</accession>
    <name>A0A452H7H1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Taste receptor type 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the G-protein coupled receptor T2R family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H7H1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010689"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012258.1">
        <property type="protein sequence ID" value="ENSGAGP00000010689.1"/>
        <property type="gene ID" value="ENSGAGG00000008338.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033038">
        <property type="term" value="F:bitter taste receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13950">
        <property type="entry name" value="7tm_TAS2R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000055">
        <property type="entry name" value="Taste receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007960">
        <property type="entry name" value="TAS2R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11394">
        <property type="entry name" value="TASTE RECEPTOR TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11394:SF149">
        <property type="entry name" value="TASTE RECEPTOR TYPE 2 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05296">
        <property type="entry name" value="TAS2R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="3 13" id="KW-0919">Taste</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="187"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="319" mass="35797" checksum="557FA72ED32E4A47" modified="2019-05-08" version="1">LLTAGLSPAAVFHLIILAIEFSAGVVTNGYIVALNCIDWAKSRTLTSYDKIITSLAFSRFCLQFFMISNVLSKVYPNIFDRFETLQPSLVIWMFTNQVSLCFASCLSVFYCVKIATFNQSLFSWLKLRLSKLVPWLLLGSILYCLVTTVAFTLFSYSYWVFSCNSTDCVSTNGTISDNKDNLLKFNFLIHSIGSISPFIVFIASSVLLIISLWRHIRKMNLNSDFNPSFRNPSTDAHVRALKSVVSFFIIYNIYYVVSTSLIGNLFYFNDELTIMVCTSIVAVYPSVHSIILIMGNPKLKLASAKILHSVSSCFGEVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7M8</accession>
    <name>A0A452H7M8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">DNA-directed RNA polymerase II subunit E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">RPB5 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H7M8"/>
    <dbReference type="SMR" id="A0A452H7M8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010713"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012283.1">
        <property type="protein sequence ID" value="ENSGAGP00000010713.1"/>
        <property type="gene ID" value="ENSGAGG00000008356.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1340.10:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1340.10">
        <property type="entry name" value="RNA polymerase, Rpb5, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005571">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036710">
        <property type="entry name" value="RNA_pol_Rpb5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03871">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000747">
        <property type="entry name" value="RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53036">
        <property type="entry name" value="Eukaryotic RPB5 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb5 N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="9">
        <location>
            <begin position="137"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="210" mass="24643" checksum="AF8C4FD1DE9C8989" modified="2019-05-08" version="1">MDDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKMYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAPKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVARYFGIKRGQVVKIIRPSETAGRYITYRLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8U9</accession>
    <name>A0A452H8U9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acylphosphatase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the acylphosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H8U9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010999"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012596.1">
        <property type="protein sequence ID" value="ENSGAGP00000010999.1"/>
        <property type="gene ID" value="ENSGAGG00000008519.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10029">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10029:SF23">
        <property type="entry name" value="ACYLPHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00112">
        <property type="entry name" value="ACYLPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="Acylphosphatase/BLUF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00151">
        <property type="entry name" value="ACYLPHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="3">
        <location>
            <begin position="31"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="123" mass="13868" checksum="E261E1C56383A84A" modified="2019-05-08" version="1">MSVQFPIPILILGIWEGSSHLQPTTLAHAVSWLPQDSGPWIMRSMGTCKYTEDQGKKLGVVGWVKNTPYGTVTGQVQGPKEKIEIMKNWLRNVGSPMSRIDRAVFSNEREISTLEFPNFTTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9E5</accession>
    <name>A0A452H9E5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H9E5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011199"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012826.1">
        <property type="protein sequence ID" value="ENSGAGP00000011199.1"/>
        <property type="gene ID" value="ENSGAGG00000008667.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="27"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="167" mass="18839" checksum="464E91B3AEDB3829" modified="2019-05-08" version="1">MEEARDYSWVKAAILDALDVSPETFWQRFRSQTYPSGIQPRLVAQALKEACRRWLQPETRTTEEIMEQVILEEFVHTLPARGQAWVLRHRLATLAAAVSLMEDFLTAETPVRPTFRPPNPRPECSNAKKKGDAPTRLRSHGCGQEACPGSRFRHPDPSPQPGPAPPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDZ7</accession>
    <name>A0A452HDZ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SERTA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HDZ7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013037"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014935.1">
        <property type="protein sequence ID" value="ENSGAGP00000013037.1"/>
        <property type="gene ID" value="ENSGAGG00000010007.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052262">
        <property type="entry name" value="E2F-SERTA_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009263">
        <property type="entry name" value="SERTA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16277">
        <property type="entry name" value="CELL DIVISION CYCLE ASSOCIATED PROTEIN 4/SERTA DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16277:SF16">
        <property type="entry name" value="SERTA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06031">
        <property type="entry name" value="SERTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51053">
        <property type="entry name" value="SERTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SERTA" evidence="1">
        <location>
            <begin position="55"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="307" mass="33968" checksum="C0D3222027AA514B" modified="2019-05-08" version="1">MLCNGAALKKRVLRKNYNTLKRFMLGRGLKRKLSDYEENMAGLSSAFDSSRNLPYPLKRQLVLNMCLTKLQTYKMLVEPNLHRSVLIANTVRQIQEEMRQESSQQPINVCSGIAPHPHSYPGIDSSGISLHLPSGSSQQESSCCNSWSAEGPIENSLLMVSDDDMSSAISSILKDLDFMEDISPPTSLIPAGDDEPLKSPENTCLKLEDDRQDLKGAECVFGSFEISNSTSYLKDLAIDDIFEDIDTSMYDSDFCSPLLMAPRPPSAATEETLKTFPSCNSPSTNNIPICRTDLSELDHIMEILVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFE3</accession>
    <name>A0A452HFE3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000013560"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015521.1">
        <property type="protein sequence ID" value="ENSGAGP00000013560.1"/>
        <property type="gene ID" value="ENSGAGG00000010352.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000021">
        <property type="entry name" value="Adenylate cyclase type 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16305:SF33">
        <property type="entry name" value="GUANYLATE CYCLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16305">
        <property type="entry name" value="TESTICULAR SOLUBLE ADENYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="617"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1257" mass="142635" checksum="C276F989A995C266" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HG11</accession>
    <name>A0A452HG11_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HG11"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013750"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015746.1">
        <property type="protein sequence ID" value="ENSGAGP00000013750.1"/>
        <property type="gene ID" value="ENSGAGG00000010480.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF1">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B, KIDNEY ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="6">
        <location>
            <begin position="104"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="7">
        <location>
            <begin position="300"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="415" mass="46674" checksum="1A4D642D0CBB788C" modified="2019-05-08" version="1">MEQFLDGVPEEIERYILDGKPKTVTEAGEIGAKWMEVAEKKKLLSRGVNTSGGHTDNKPYNRGQHKTPPTTQGKPQTTYYPTSPVSSNPPRPSDQSAGQCFKCNELGHIKIAAQICRQAGLVKKSKDVMDYHEENFAIVFAAMGVNMETARFFKSDFEENGSMENVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNDITHPIPDLTGFITEGQVYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHGDVSNQLYACYAIGKDVQAMKAVVGEEALSADDLLYLEFLQKFEKQFIAQGPYDNRTIFESLNIGWQLLRIFPKELLKRIPESILAEYYPREAKAPGLQHHDTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHG3</accession>
    <name>A0A452HHG3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Metalloendopeptidase</fullName>
            <ecNumber evidence="14">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13 14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasmic vesicle</location>
            <location evidence="11">Secretory vesicle</location>
            <location evidence="11">Cortical granule</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A452HHG3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014313"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016376.1">
        <property type="protein sequence ID" value="ENSGAGP00000014313.1"/>
        <property type="gene ID" value="ENSGAGG00000010817.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060473">
        <property type="term" value="C:cortical granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04283">
        <property type="entry name" value="ZnMc_hatching_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000040">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034039">
        <property type="entry name" value="ZnMP_hatching_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF899">
        <property type="entry name" value="ASTACIN-LIKE METALLOENDOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0278">Fertilization</keyword>
    <keyword evidence="5 13 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13 14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 13 14" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6 13 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="16">
        <location>
            <begin position="62"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="15">
        <location>
            <begin position="264"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="264"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="429" mass="46943" checksum="85D0AFEE689A49E9" modified="2019-05-08" version="1">QASFGWQPLRVCGQASCTNNPREEQVWEGWSFLVNTKQAPLKASCFSQRLREGDIVVTKSRSAIGCSDHSCFWPQSSDGIVRVPYVFSPDYDEAQIRWINEAMAEFEILTCIDFVNHTTESDYLSIKSGKGCWSHYGKIGGGQILSLMKKGCMWKGVIQHELNHALGFLHEQSRSDRDSYVKIMWEYISAGNKGNFKKSENSNNLGLQYDYSSVMHYGTNAFSNTPGRATIIPIPDASVPIGQRYGLSNLDVAKINKLYNCNRCSTVLDSPSGSLSSDNHTSKYSSSNATCFWLIRIPSKRVSLQFDAFSLKSSTDCKTEYVKIYDGVSQSSEVLLDKTCGTKSPPVVTASGRTVLVEFVRGGTGAATGFKISYSSGRSNPRKNGSINLYPSPGIAATSLAATLSAGHSELKGNMCPPSSTSSRFGILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHG9</accession>
    <name>A0A452HHG9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HHG9"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016390.1">
        <property type="protein sequence ID" value="ENSGAGP00000014324.1"/>
        <property type="gene ID" value="ENSGAGG00000010891.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050350">
        <property type="entry name" value="Compl-Cell_Adhes-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325:SF570">
        <property type="entry name" value="COMPLEMENT COMPONENT 4 BINDING PROTEIN, MEMBRANE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325">
        <property type="entry name" value="COMPLEMENT COMPONENT-RELATED SUSHI DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0768">Sushi</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="9"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="83"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="136"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="197"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="199"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="372" mass="40822" checksum="93108904D3770A8B" modified="2019-05-08" version="1">MCSYVFLVGQCRAPPTLSRAVPRDTNGRESFPIRTEVTYKCHDGFVKIPGKSDTVVCLSTSEWSTIGEFCNASCGAAPRLKSAALRKEDEAKNYCPPGSTMNYVCRPGYESTELRPVITCLENLTWSEAPEFCRGKSCGVPKGPEHGRAVVLTNDQYGARADIICDEGFRLHGQTFIQCLLKGDQVEWRQLPTCEAITCSPPPNIAKGMHNGSNVEIFVYNSLVTYKCDHNYSLTGEASIHCTAKDNISGVWNGSAPECKGQCGPNQRLHRVVHDTNLIGGFTTNREVICSALNTAGNSAEPEGGGPGFDTRIGIPAIAIAFAISVIIIVYIRKYQKKKCSYSVNPDSSKHQITMEHDFHMNESKHNLIFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHK9</accession>
    <name>A0A452HHK9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="10">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the quiescin-sulfhydryl oxidase (QSOX) family.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452HHK9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014381"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016451.1">
        <property type="protein sequence ID" value="ENSGAGP00000014381.1"/>
        <property type="gene ID" value="ENSGAGG00000010928.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02992">
        <property type="entry name" value="PDI_a_QSOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1960:FF:000001">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000001">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000073">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000080">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1960">
        <property type="entry name" value="QSOX sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040986">
        <property type="entry name" value="QSOX_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042568">
        <property type="entry name" value="QSOX_FAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041269">
        <property type="entry name" value="QSOX_Trx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039798">
        <property type="entry name" value="Sulfhydryl_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22897">
        <property type="entry name" value="QUIESCIN Q6-RELATED SULFHYDRYL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22897:SF7">
        <property type="entry name" value="SULFHYDRYL OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18371">
        <property type="entry name" value="FAD_SOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18108">
        <property type="entry name" value="QSOX_Trx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0274">FAD</keyword>
    <keyword evidence="3 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019220117" description="Sulfhydryl oxidase" evidence="11">
        <location>
            <begin position="25"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="649"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="13">
        <location>
            <begin position="17"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="12">
        <location>
            <begin position="404"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="682" mass="77267" checksum="A9ECBA6E9E4C918F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIL3</accession>
    <name>A0A452HIL3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LIM domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the zyxin/ajuba family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HIL3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014708"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016816.1">
        <property type="protein sequence ID" value="ENSGAGP00000014708.1"/>
        <property type="gene ID" value="ENSGAGG00000011157.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000016817.1">
        <property type="protein sequence ID" value="ENSGAGP00000014709.1"/>
        <property type="gene ID" value="ENSGAGG00000011157.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035331">
        <property type="term" value="P:negative regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09352">
        <property type="entry name" value="LIM1_Ajuba_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09355">
        <property type="entry name" value="LIM2_Ajuba_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09438">
        <property type="entry name" value="LIM3_Ajuba_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000028">
        <property type="entry name" value="LIM domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000036">
        <property type="entry name" value="LIM domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000037">
        <property type="entry name" value="LIM domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047172">
        <property type="entry name" value="Ajuba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047245">
        <property type="entry name" value="Ajuba-like_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047247">
        <property type="entry name" value="Ajuba-like_LIM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047248">
        <property type="entry name" value="Ajuba-like_LIM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24219:SF3">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24219">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN JUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 14" id="KW-0440">LIM domain</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="16">
        <location>
            <begin position="503"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="16">
        <location>
            <begin position="568"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="16">
        <location>
            <begin position="629"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="175"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="434"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="252"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="266"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="314"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="443"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="709" mass="77769" checksum="8DA78F8DCA2127A3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIW0</accession>
    <name>A0A452HIW0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cysteine-rich and transmembrane domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CYSTM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HIW0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014844"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016961.1">
        <property type="protein sequence ID" value="ENSGAGP00000014844.1"/>
        <property type="gene ID" value="ENSGAGG00000011240.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043240">
        <property type="entry name" value="CYSTM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028144">
        <property type="entry name" value="CYSTM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47564">
        <property type="entry name" value="CYSTEINE-RICH AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47564:SF1">
        <property type="entry name" value="CYSTEINE-RICH AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12734">
        <property type="entry name" value="CYSTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cysteine-rich transmembrane" evidence="6">
        <location>
            <begin position="79"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="113" mass="12150" checksum="4D2D655DB8BBF9E8" modified="2019-05-08" version="1">MNYENPPPYPGPGPTAPYPPYTQQPGGPPGPSPYPGYPPGPAGPYQPGQPGYQGYPQYGWQNAPPPPGPMYADGPKNTVYVVEEQRRDNTGESACLTACWTALCCCCLWDMLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJ51</accession>
    <name>A0A452HJ51_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJ51"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014903"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017023.1">
        <property type="protein sequence ID" value="ENSGAGP00000014903.1"/>
        <property type="gene ID" value="ENSGAGG00000011269.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033549">
        <property type="term" value="F:MAP kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14576">
        <property type="entry name" value="DSP_iDUSP27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020405">
        <property type="entry name" value="Atypical_DUSP_subfamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682">
        <property type="entry name" value="AGAP008228-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682:SF4">
        <property type="entry name" value="SERINE_THREONINE_TYROSINE-INTERACTING-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01909">
        <property type="entry name" value="ADSPHPHTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="132"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="201"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="357"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="500"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="570"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="677"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="808"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="885"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="955"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="509"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="687"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="700"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="817"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="892"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="955"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1008"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1019"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1048"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1094"/>
            <end position="1106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1153" mass="129853" checksum="1B697D1C1B70D812" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJC6</accession>
    <name>A0A452HJC6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FERM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJC6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015015"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017150.1">
        <property type="protein sequence ID" value="ENSGAGP00000015015.1"/>
        <property type="gene ID" value="ENSGAGG00000011346.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13194">
        <property type="entry name" value="FERM_C_ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17187">
        <property type="entry name" value="FERM_F1_ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000003">
        <property type="entry name" value="Radixin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000002">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000013">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.450:FF:000001">
        <property type="entry name" value="radixin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011174">
        <property type="entry name" value="ERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011259">
        <property type="entry name" value="ERM_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041789">
        <property type="entry name" value="ERM_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046810">
        <property type="entry name" value="ERM_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008954">
        <property type="entry name" value="Moesin_tail_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23281">
        <property type="entry name" value="MERLIN/MOESIN/EZRIN/RADIXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00769">
        <property type="entry name" value="ERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20492">
        <property type="entry name" value="ERM_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002305">
        <property type="entry name" value="ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48678">
        <property type="entry name" value="Moesin tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="6">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="373"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="373"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="455"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="523"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="49"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR002305-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="569" mass="67078" checksum="7284FAAAC331812F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJE9</accession>
    <name>A0A452HJE9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJE9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015036"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017180.1">
        <property type="protein sequence ID" value="ENSGAGP00000015036.1"/>
        <property type="gene ID" value="ENSGAGG00000011366.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000321">
        <property type="entry name" value="myeloid zinc finger 1 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000058">
        <property type="entry name" value="Zinc finger protein 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000895">
        <property type="entry name" value="Zinc finger protein 597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000628">
        <property type="entry name" value="zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000933">
        <property type="entry name" value="zinc finger protein 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF218">
        <property type="entry name" value="ZINC FINGER PROTEIN 502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="379"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="454"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="482"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="538"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="566"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="590" mass="68387" checksum="BDCFDB3F1E5ED59D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HM39</accession>
    <name>A0A452HM39_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tyrosine 3-monooxygenase</fullName>
            <ecNumber evidence="6">1.14.16.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Tyrosine 3-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.2" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452HM39"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015995"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018275.1">
        <property type="protein sequence ID" value="ENSGAGP00000015995.1"/>
        <property type="gene ID" value="ENSGAGG00000012021.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00747">
        <property type="reaction ID" value="UER00733"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004511">
        <property type="term" value="F:tyrosine 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006585">
        <property type="term" value="P:dopamine biosynthetic process from tyrosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04930">
        <property type="entry name" value="ACT_TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03345">
        <property type="entry name" value="eu_TyrOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.800.10:FF:000002">
        <property type="entry name" value="Tyrosine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000024">
        <property type="entry name" value="Tyrosine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.800.10">
        <property type="entry name" value="Aromatic amino acid hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001273">
        <property type="entry name" value="ArAA_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018301">
        <property type="entry name" value="ArAA_hydroxylase_Fe/CU_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036951">
        <property type="entry name" value="ArAA_hydroxylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036329">
        <property type="entry name" value="Aro-AA_hydroxylase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019774">
        <property type="entry name" value="Aromatic-AA_hydroxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041903">
        <property type="entry name" value="Eu_TyrOH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049321">
        <property type="entry name" value="TH_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005962">
        <property type="entry name" value="Tyr_3_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019773">
        <property type="entry name" value="Tyrosine_3-monooxygenase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021164">
        <property type="entry name" value="Tyrosine_hydroxylase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01269">
        <property type="entry name" value="Tyr_3_monoox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473">
        <property type="entry name" value="AROMATIC AMINO ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473:SF18">
        <property type="entry name" value="TYROSINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00351">
        <property type="entry name" value="Biopterin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21417">
        <property type="entry name" value="TH_ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12549">
        <property type="entry name" value="TOH_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000336">
        <property type="entry name" value="TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00372">
        <property type="entry name" value="FYWHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56534">
        <property type="entry name" value="Aromatic aminoacid monoxygenases, catalytic and oligomerization domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00367">
        <property type="entry name" value="BH4_AAA_HYDROXYL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51410">
        <property type="entry name" value="BH4_AAA_HYDROXYL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 18" id="KW-0408">Iron</keyword>
    <keyword evidence="11 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="12" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biopterin-dependent aromatic amino acid hydroxylase family profile" evidence="20">
        <location>
            <begin position="174"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="520" mass="59197" checksum="389B845ECEE9BF1D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMD0</accession>
    <name>A0A452HMD0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome c oxidase assembly factor 6 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the maturation of the mitochondrial respiratory chain complex IV subunit MT-CO2/COX2. Thereby, may regulate early steps of complex IV assembly. Mitochondrial respiratory chain complex IV or cytochrome c oxidase is the component of the respiratory chain that catalyzes the transfer of electrons from intermembrane space cytochrome c to molecular oxygen in the matrix and as a consequence contributes to the proton gradient involved in mitochondrial ATP synthesis. May also be required for efficient formation of respiratory supercomplexes comprised of complexes III and IV.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c oxidase subunit 6B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HMD0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016102"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018388.1">
        <property type="protein sequence ID" value="ENSGAGP00000016102.1"/>
        <property type="gene ID" value="ENSGAGG00000012090.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042775">
        <property type="term" value="P:mitochondrial ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008535">
        <property type="term" value="P:respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.140:FF:000002">
        <property type="entry name" value="Cytochrome c oxidase assembly factor 6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042289">
        <property type="entry name" value="COA6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46690">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46690:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="115" mass="13123" checksum="8A27F8EE0A27B401" modified="2019-05-08" version="1">RHPGAPCCSPGGGAAFSPPRGSGGHLQIYFILPVIKMSAPSMEERKACWGARDEYWKCLDENMEDASRCQKLRCSFESRCPQQWVKYFDKRRDFLKYKEQLQAAEYQPPGATEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMH0</accession>
    <name>A0A452HMH0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Pro-neuregulin-2, membrane-bound isoform</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. May also promote the heterodimerization with the EGF receptor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with ERBB3 and ERBB4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the neuregulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HMH0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016147"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018437.1">
        <property type="protein sequence ID" value="ENSGAGP00000016147.1"/>
        <property type="gene ID" value="ENSGAGG00000012118.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05750">
        <property type="entry name" value="Ig_Pro_neuregulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000116">
        <property type="entry name" value="pro-neuregulin-2, membrane-bound isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000354">
        <property type="entry name" value="Pro-neuregulin-2, membrane-bound isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040180">
        <property type="entry name" value="Neuregulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002154">
        <property type="entry name" value="Neuregulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057911">
        <property type="entry name" value="NRG2_Ig-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11100">
        <property type="entry name" value="HEREGULIN-NEUREGULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11100:SF20">
        <property type="entry name" value="PRO-NEUREGULIN-2, MEMBRANE-BOUND ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02158">
        <property type="entry name" value="Neuregulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0339">Growth factor</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="21">
        <location>
            <begin position="123"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="227"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="378"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="586"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="380"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="402"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="414"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="618"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="639"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="669"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="683"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="694" mass="77712" checksum="7DFED99A1DF81377" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMX6</accession>
    <name>A0A452HMX6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Integral membrane protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the ITM2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HMX6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016312"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018619.1">
        <property type="protein sequence ID" value="ENSGAGP00000016312.1"/>
        <property type="gene ID" value="ENSGAGG00000012228.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042985">
        <property type="term" value="P:negative regulation of amyloid precursor protein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007084">
        <property type="entry name" value="BRICHOS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040145">
        <property type="entry name" value="ITM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10962:SF4">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10962">
        <property type="entry name" value="INTEGRAL TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04089">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01039">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50869">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="BRICHOS" evidence="15">
        <location>
            <begin position="131"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="261" mass="29953" checksum="6BF461AB1B9B8ACF" modified="2019-05-08" version="1">MVKVSFNSALAQKEAAKKDEENNQVLILGPDPKDPEAVVPVGQRRAWCWCMCFGVAFMLAGVILGGAYLYKYFAFQQGGVYFCGIKYIEDDLTLTEPDGRAPAALYQTIEQNIQILEEEDVEFISVPVPEFADSDPADIVHDFHRRLTAYLDLSLDKCYVIPLNTSIVMPPKNFMELLINIKAGTYLPQSYLIHEQMIVTDRIENVDQLGFFIYRLCRGKETYKLQRKETMKGIQKREATNCRKIRHFENRFAIETLICEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HP36</accession>
    <name>A0A452HP36_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HP36"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016733"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019104.1">
        <property type="protein sequence ID" value="ENSGAGP00000016733.1"/>
        <property type="gene ID" value="ENSGAGG00000012502.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000434">
        <property type="entry name" value="Transcription termination factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053078">
        <property type="entry name" value="TTF1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46760">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46760:SF1">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="465"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="465"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="707" mass="82228" checksum="7D4CFBE79BEC50FA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQ49</accession>
    <name>A0A452HQ49_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts preferentially with RAC2. Interacts with RAC1. Interacts with AUTS2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000017146"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019569.1">
        <property type="protein sequence ID" value="ENSGAGP00000017146.1"/>
        <property type="gene ID" value="ENSGAGG00000012802.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045321">
        <property type="term" value="P:leukocyte activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072593">
        <property type="term" value="P:reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04440">
        <property type="entry name" value="DEP_2_P-Rex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01224">
        <property type="entry name" value="PH_Collybistin_ASEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000055">
        <property type="entry name" value="Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000018">
        <property type="entry name" value="Phosphatidylinositol-3,4, 5-trisphosphate-dependent Rac exchange factor 2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000090">
        <property type="entry name" value="Phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000094">
        <property type="entry name" value="Phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000099">
        <property type="entry name" value="Phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051832">
        <property type="entry name" value="mTOR-Rac_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22829">
        <property type="entry name" value="DEP DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22829:SF6">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE-DEPENDENT RAC EXCHANGER 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DH" evidence="14">
        <location>
            <begin position="27"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="249"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="15">
        <location>
            <begin position="398"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="15">
        <location>
            <begin position="506"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1097"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1649" mass="187377" checksum="2C7BC37361B59483" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRT3</accession>
    <name>A0A452HRT3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sodium/potassium-transporting ATPase subunit beta-1-interacting protein</fullName>
            <shortName evidence="7">Na(+)/K(+)-transporting ATPase subunit beta-1-interacting protein</shortName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NKAIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HRT3"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020272.1">
        <property type="protein sequence ID" value="ENSGAGP00000017772.1"/>
        <property type="gene ID" value="ENSGAGG00000013194.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002028">
        <property type="term" value="P:regulation of sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008516">
        <property type="entry name" value="Na/K-Atpase_Interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13084:SF3">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-1-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13084">
        <property type="entry name" value="T-CELL LYMPHOMA BREAKPOINT-ASSOCIATED TARGET 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05640">
        <property type="entry name" value="NKAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="81" mass="9537" checksum="7B0E7FFB5F5FFA38" modified="2019-05-08" version="1">MQIFLVCVLERQIFDFLGYQWAPILANFVHIIIVILGLFGTIQYRPRYITGVSIIYVFFSESLMILSQVSYLEFFIKSHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRX9</accession>
    <name>A0A452HRX9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">V-type proton ATPase subunit C</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5 6">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and two accessory subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the V-ATPase C subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HRX9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017805"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020316.1">
        <property type="protein sequence ID" value="ENSGAGP00000017805.1"/>
        <property type="gene ID" value="ENSGAGG00000013219.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14785">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1460.10:FF:000004">
        <property type="entry name" value="V-type proton ATPase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000002">
        <property type="entry name" value="V-type proton ATPase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1460.10">
        <property type="entry name" value="subunit c (vma5p) of the yeast v-atpase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1180">
        <property type="entry name" value="Vacuolar atp synthase subunit c, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004907">
        <property type="entry name" value="ATPase_V1-cplx_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036132">
        <property type="entry name" value="Vac_ATP_synth_c_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03223">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118203">
        <property type="entry name" value="Vacuolar ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="392" mass="44888" checksum="5C783136CB1920B6" modified="2019-05-08" version="1">MSEFWLISAPGDKVNLQALERMNTVTSKANLSSNNKFLIPDLKVGTLDALVGLSDELGKLDSFAESVIKKIAQYIGEVMEDSKDKVQENLLANGGLKNKMKCLKIDLISYLTRFEWDMAKYPIKQPLKNISEALAKQITQIETDLKSRTAAYNNIKGNLQSLERKTVGNLLTRTLADIVNKEDFVLNSEYLITLLVVVPKSNYAQWQKTYESLSDMVVPRSTKMIAEDAEGGLFTVTLFRKVMDDFKAKARENRFMVREFYFDEKELKCEKEEIMKLASDKKQQYGPLLRWLKVNFSEAFVAWIHVKALRVFVESVLRYGLPVNFQAMLLQPSKKSVKRLRDVLNAVFKHLDEVAAASIMDMSMDIPGLQLSNQEYYPYVYFKIDLSLLDYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSY8</accession>
    <name>A0A452HSY8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000018205"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020751.1">
        <property type="protein sequence ID" value="ENSGAGP00000018205.1"/>
        <property type="gene ID" value="ENSGAGG00000013489.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000020754.1">
        <property type="protein sequence ID" value="ENSGAGP00000018208.1"/>
        <property type="gene ID" value="ENSGAGG00000013489.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040010">
        <property type="entry name" value="ZN608/ZN609"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21564">
        <property type="entry name" value="BRAKELESS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21564:SF4">
        <property type="entry name" value="ZINC FINGER PROTEIN 608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="549"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="611"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="771"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="922"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1110"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1246"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1416"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="520"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="643"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="775"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="812"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="922"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="945"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="955"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="974"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1002"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1048"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1118"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1139"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1175"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1191"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1224"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1246"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1284"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1430"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1451"/>
            <end position="1460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1505" mass="162694" checksum="BE6FBFB639490E2B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUF5</accession>
    <name>A0A452HUF5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NALF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HUF5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018691"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021308.1">
        <property type="protein sequence ID" value="ENSGAGP00000018691.1"/>
        <property type="gene ID" value="ENSGAGG00000013837.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015275">
        <property type="term" value="F:stretch-activated, monoatomic cation-selective, calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055288">
        <property type="entry name" value="NALCN_aux_factor_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15819:SF9">
        <property type="entry name" value="NALCN CHANNEL AUXILIARY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15819">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN FAM155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="68"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="325"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="381" mass="42385" checksum="49FA4B8B5A972138" modified="2019-05-08" version="1">MTRGAWMCSRQYDDGLKICFAPRENEKPFTDSERAQKWRLSLASLLFFTVLLSDHLWFCAEAKLTRTRDKEQQQQQQPEHPERQLLSSMGEPSPAAQAAHRLLSSSLPPLPPSPSSSCSSGGGSSNNNNSNNNNKAIFLGNSTKPFWRLETCYPQSSASGQCFSVEDADSVCRRRWSREQQVTGNHPTPSWNLTDFYLSFCNSYTLWELFTGLSNPDTLNCSLDVVLEGEGSCSQCVQAYQRYDQHAQEKYEEFEVMLQKYLQSDEYSVNSCPEDCKVGALLCFMLSFIDGPAGCFPFCVWRRPWGSRPGSCSCHAAVTRGSIDSCNPQGPGLEPGVEGGPGFPPNLPTPDQTQEELTQTVGKTVGKITEVSKSANKHRTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUR6</accession>
    <name>A0A452HUR6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JIP scaffold family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HUR6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018863"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021502.1">
        <property type="protein sequence ID" value="ENSGAGP00000018863.1"/>
        <property type="gene ID" value="ENSGAGG00000013951.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007254">
        <property type="term" value="P:JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046328">
        <property type="term" value="P:regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01212">
        <property type="entry name" value="PTB_JIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11943">
        <property type="entry name" value="SH3_JIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000108">
        <property type="entry name" value="C-Jun-amino-terminal kinase-interacting protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000032">
        <property type="entry name" value="Putative C-Jun-amino-terminal kinase-interacting protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047178">
        <property type="entry name" value="JIP1_scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035638">
        <property type="entry name" value="JIP1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47437:SF3">
        <property type="entry name" value="C-JUN-AMINO-TERMINAL KINASE-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47437">
        <property type="entry name" value="JNK-INTERACTING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="389"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="8">
        <location>
            <begin position="467"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="186"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="613" mass="68962" checksum="6AFFA0B5F6183E15" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUX1</accession>
    <name>A0A452HUX1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Diacylglycerol kinase</fullName>
            <shortName evidence="12">DAG kinase</shortName>
            <ecNumber evidence="12">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:40328"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; glycerolipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452HUX1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018910"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021565.1">
        <property type="protein sequence ID" value="ENSGAGP00000018910.1"/>
        <property type="gene ID" value="ENSGAGG00000013179.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00230"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20845">
        <property type="entry name" value="C1_DGKbeta_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000003">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000013">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000016">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000003">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047471">
        <property type="entry name" value="C1_DGKbeta-like_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF32">
        <property type="entry name" value="DIACYLGLYCEROL KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019201674" description="Diacylglycerol kinase" evidence="14">
        <location>
            <begin position="22"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="15">
        <location>
            <begin position="76"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="15">
        <location>
            <begin position="141"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="DAGKc" evidence="16">
        <location>
            <begin position="254"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="493"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="498"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="624" mass="70117" checksum="93CB1816D7AC43A4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVB4</accession>
    <name>A0A452HVB4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL15m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L15, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HVB4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019086"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021754.1">
        <property type="protein sequence ID" value="ENSGAGP00000019086.1"/>
        <property type="gene ID" value="ENSGAGG00000014120.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000006">
        <property type="entry name" value="39S ribosomal protein L15, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_uL15_bac-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934:SF11">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="10">
        <location>
            <begin position="170"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="99"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="371" mass="41371" checksum="F2B39E14387EC994" modified="2019-05-08" version="1">MDTHYTTRHLTRPPLPRSPPALSHRPARPPRHGRVATVGRGGGSGGRKATRCSPPLLPLQAGCVRQRERAAMAAASRGGGGGGPKALELLRALPRVSLVNLRPNPGAKKSEKRRGRGRHGGKKSGRGHKGQRQRGTRPRLGFEGGQTPFYLIIPKYGYNEGHSRRRQYHPLSLQKLQYLIDLGRVDPTQPIDLTQLTNARGVTIQPLKRDYGVQLVEEGADIFSAKVNIEVQMASELAIAAIEKNGGVITTGFYDPRSLEILCKPIPFFLRGQPIPKRMLPPEDLVRYYTDARNRGYLANPSNIPEARLELAKKYGYVLPDISKDELFRMLSTRKDPRQIFFGLAPGWVVNMSEKKILKPTDERLLRYYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVD8</accession>
    <name>A0A452HVD8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm protein F</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HVD8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019106"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021774.1">
        <property type="protein sequence ID" value="ENSGAGP00000019106.1"/>
        <property type="gene ID" value="ENSGAGG00000014132.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01722">
        <property type="entry name" value="Sm_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034100">
        <property type="entry name" value="Sm_F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 10" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="6"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="83" mass="9376" checksum="83589802BBBD2A14" modified="2019-05-08" version="1">MSLSLNPNPFLNGLTGKLVMVKLKWRMEYKGYLVSVNGYVNMQLANTEEYIDGALSGHLGEVLIKCNDMSSTSEEEKEEKMEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWV9</accession>
    <name>A0A452HWV9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="4">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019693"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022434.1">
        <property type="protein sequence ID" value="ENSGAGP00000019693.1"/>
        <property type="gene ID" value="ENSGAGG00000014526.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003990">
        <property type="term" value="F:acetylcholinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006581">
        <property type="term" value="P:acetylcholine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019695">
        <property type="term" value="P:choline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050654">
        <property type="entry name" value="AChE-related_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918">
        <property type="entry name" value="ACETYLCHOLINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918:SF4">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018818344" description="Carboxylic ester hydrolase" evidence="4">
        <location>
            <begin position="26"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="5">
        <location>
            <begin position="30"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="403" mass="43601" checksum="D8E44122970945C4" modified="2019-05-08" version="1">MLGLGLLPALPGLLLLSLLCPGSSSDDDGTVVLTTSGPIWGKHLQAGSGTVTAFLGIPYAEPPVGALRFQKPLPHQPWSEVLETTRFGKPCHQAQFPSHPDXPGVPFPSHPDAYMWTPKMPQSKDCLFLNVWFFGGAASVELQDGHFLAATENMIMASMNYWLGALGFLSLPPAAPGNAGLWDQRLAQRWLQDNAAAFRGDPARLLLFGQSAGAASVGFHLLSLGSLFTRTTLQSGAPTSPWAWFSQEVAKKIGWQYSQEGQGPAWHQRTMPHVISDYHGVCPLAKTAGQMAEAGSPVCVYTFTHRPSGLSSPKWTAVPHASEMPYLFRTLASVGRANHPHTEDEMALSCRVMLATLSASQPGSTCTFSFPLMDQSSMNLSNYLKTQLYFWPSQHPLARSSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXY8</accession>
    <name>A0A452HXY8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 17">Alpha-(1,6)-fucosyltransferase</fullName>
            <ecNumber evidence="4 17">2.4.1.68</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 17">Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + GDP-beta-L-fucose = an N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-[alpha-L-Fuc-(1-&gt;6)]-beta-D-GlcNAc}-L-asparaginyl-[protein] + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12985"/>
            <dbReference type="Rhea" id="RHEA-COMP:13526"/>
            <dbReference type="Rhea" id="RHEA-COMP:13532"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:60651"/>
            <dbReference type="ChEBI" id="CHEBI:137207"/>
            <dbReference type="EC" id="2.4.1.68"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17 22">Belongs to the glycosyltransferase 23 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.68" evidence="4 17"/>
    <dbReference type="AlphaFoldDB" id="A0A452HXY8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019995"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022779.1">
        <property type="protein sequence ID" value="ENSGAGP00000019995.1"/>
        <property type="gene ID" value="ENSGAGG00000014729.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008424">
        <property type="term" value="F:glycoprotein 6-alpha-L-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11300">
        <property type="entry name" value="Fut8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11792">
        <property type="entry name" value="SH3_Fut8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1060:FF:000003">
        <property type="entry name" value="Alpha-(1,6)-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000070">
        <property type="entry name" value="Alpha-(1,6)-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11350:FF:000001">
        <property type="entry name" value="Alpha-(1,6)-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015827">
        <property type="entry name" value="Fut8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045573">
        <property type="entry name" value="Fut8_N_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035653">
        <property type="entry name" value="Fut8_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027350">
        <property type="entry name" value="GT23_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13132">
        <property type="entry name" value="ALPHA- 1,6 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13132:SF29">
        <property type="entry name" value="ALPHA-(1,6)-FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19745">
        <property type="entry name" value="FUT8_N_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000472">
        <property type="entry name" value="Alpha1_6FUT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51659">
        <property type="entry name" value="GT23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 17 22" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 17 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="13" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8 17 22" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="GT23" evidence="26">
        <location>
            <begin position="207"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="25">
        <location>
            <begin position="503"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Important for donor substrate binding" evidence="18 22">
        <location>
            <begin position="366"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="43"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="SH3-binding" evidence="19">
        <location>
            <begin position="300"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="205"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="219"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="466"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000472-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000472-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000472-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="576" mass="66491" checksum="51D1C6168081A5BB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYF0</accession>
    <name>A0A452HYF0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000020161"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022975.1">
        <property type="protein sequence ID" value="ENSGAGP00000020161.1"/>
        <property type="gene ID" value="ENSGAGG00000014744.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46726">
        <property type="entry name" value="TWO PORE CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46726:SF1">
        <property type="entry name" value="TWO-PORE CALCIUM CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="468"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="529"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="612"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="637"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="66"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="411"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="777" mass="90246" checksum="C84B207D175DB079" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZ20</accession>
    <name>A0A452HZ20_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DnaJ homolog subfamily C member 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Required for mitochondrial inner membrane organization. Seems to function through its association with the MICOS complex and the mitochondrial outer membrane sorting assembly machinery (SAM) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DNAJC11 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZ20"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020430"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023279.1">
        <property type="protein sequence ID" value="ENSGAGP00000020430.1"/>
        <property type="gene ID" value="ENSGAGG00000015056.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000027">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily C, member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024586">
        <property type="entry name" value="DnaJ-like_C11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055225">
        <property type="entry name" value="DNAJC11-like_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052243">
        <property type="entry name" value="Mito_inner_membrane_organizer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44157">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44157:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11875">
        <property type="entry name" value="DnaJ-like_C11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22774">
        <property type="entry name" value="DNAJC11_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="13">
        <location>
            <begin position="14"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="417"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="559" mass="63252" checksum="10A6D44838A54D3C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0H2</accession>
    <name>A0A452I0H2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Neuronal cell adhesion molecule</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Neuronal surface protein Bravo</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">NgCAM-related cell adhesion molecule</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000021006"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023927.1">
        <property type="protein sequence ID" value="ENSGAGP00000021006.1"/>
        <property type="gene ID" value="ENSGAGG00000015436.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd05731">
        <property type="entry name" value="Ig3_L1-CAM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05868">
        <property type="entry name" value="Ig4_NrCAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05874">
        <property type="entry name" value="IgI_NrCAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000057">
        <property type="entry name" value="neural cell adhesion molecule L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000363">
        <property type="entry name" value="neurofascin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000512">
        <property type="entry name" value="neurofascin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000005">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000038">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000078">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000114">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000347">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000836">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000100">
        <property type="entry name" value="Neuronal cell adhesion molecule a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000332">
        <property type="entry name" value="neuronal cell adhesion molecule isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026966">
        <property type="entry name" value="Neurofascin/L1/NrCAM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF257">
        <property type="entry name" value="NEURAL CELL ADHESION MOLECULE L1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13882">
        <property type="entry name" value="Bravo_FIGEY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019363761" description="Neuronal cell adhesion molecule" evidence="18">
        <location>
            <begin position="25"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1186"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="41"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="140"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="263"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="356"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="449"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="540"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="644"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="741"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="843"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="949"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="1081"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1216"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1216"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1262"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1309"/>
            <end position="1325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1325" mass="146602" checksum="777C6011278936ED" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0L5</accession>
    <name>A0A452I0L5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FHF complex subunit HOOK-interacting protein 1B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FHIP1B, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. The FHF complex associates with the homotypic vesicular sorting complex (the HOPS complex).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FHIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I0L5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021046"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023968.1">
        <property type="protein sequence ID" value="ENSGAGP00000021046.1"/>
        <property type="gene ID" value="ENSGAGG00000015456.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070695">
        <property type="term" value="C:FHF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019384">
        <property type="entry name" value="FHIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045669">
        <property type="entry name" value="FHIP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045668">
        <property type="entry name" value="FHIP_KELAA_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21705:SF4">
        <property type="entry name" value="FHF COMPLEX SUBUNIT HOOK-INTERACTING PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21705">
        <property type="entry name" value="RAI16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19314">
        <property type="entry name" value="DUF5917"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19311">
        <property type="entry name" value="KELAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10257">
        <property type="entry name" value="RAI16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FHF complex subunit HOOK-interacting protein C-terminal" evidence="8">
        <location>
            <begin position="709"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="428"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="491"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="642"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="399"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="555"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF19314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="908" mass="98895" checksum="127F1CCD1B6F51D1" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3L2</accession>
    <name>A0A452I3L2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Neurotransmitter-gated ion-channel transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3L2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022081"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025168.1">
        <property type="protein sequence ID" value="ENSGAGP00000022081.1"/>
        <property type="gene ID" value="ENSGAGG00000016214.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19059">
        <property type="entry name" value="LGIC_TM_GABAAR_rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000005">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit rho-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="18">
        <location>
            <begin position="108"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="297" mass="34612" checksum="411DF128371D91F9" modified="2019-05-08" version="1">MPSLACVPQVANPWNKSWTESILFGIIALHQRFTPIFLDAYTEDDLMLYWKNGNDSLKTDERISLSQFLIQEFHTTTKLAFYSSTGWYNRLYINFTLRRHIFFFLLQTYFPATLMVMLSWVSFWIDRRAVPARVPLGITTVLTMSTIITGVNASMPRVSYIKAVDIYLWVSFVFVFLSVLEYAAVNYLTTVQERKERKLRDKLPCTCGLPQPRPMMMDGSYSDGDVNELGHYVSENGDKPDRMMMQLTLGSERSSSRRKSQRYVSMRIDTHAIDKYSRIIFPGSYILFNLIYWSIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4B0</accession>
    <name>A0A452I4B0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BLOC-1-related complex subunit 6 C-terminal helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I4B0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022355"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025472.1">
        <property type="protein sequence ID" value="ENSGAGP00000022355.1"/>
        <property type="gene ID" value="ENSGAGG00000016407.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019314">
        <property type="entry name" value="BORCS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046465">
        <property type="entry name" value="BORCS6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13440">
        <property type="entry name" value="BLOC-1 RELATED COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13440:SF7">
        <property type="entry name" value="BLOC-1 RELATED COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10157">
        <property type="entry name" value="BORCS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BLOC-1-related complex subunit 6 C-terminal helix" evidence="2">
        <location>
            <begin position="105"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="208" mass="22537" checksum="CA56822CDAE8683C" modified="2019-05-08" version="1">MDIAKEQQAAAAGDRLAGGRLLDSRSLDGISQAYGASRAQEGRRATISSALELEGTVSHDGDLTHFVANNLQLKIKLSSRGSLEEPEPPGQPFAGLGRSKAADIPPIDPAVLAELERLTQEVAHKVDQMMRSLSGSIQNMTALSVGYIQTYRDAVDSLGESVDMSIKGMYTLMARGEELDRSMQPVHALAKQIREIKRTLEIFESLCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4F9</accession>
    <name>A0A452I4F9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Actin-like protein 6A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">BRG1-associated factor 53A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the actin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I4F9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022439"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025566.1">
        <property type="protein sequence ID" value="ENSGAGP00000022439.1"/>
        <property type="gene ID" value="ENSGAGG00000016463.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071564">
        <property type="term" value="C:npBAF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033044">
        <property type="term" value="P:regulation of chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13395">
        <property type="entry name" value="ASKHA_NBD_Arp4_ACTL6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.36.70:FF:000004">
        <property type="entry name" value="Actin-like 6A, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000009">
        <property type="entry name" value="Actin-like 6A, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000375">
        <property type="entry name" value="Actin-related protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.36.70">
        <property type="entry name" value="Actin, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00072655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00076522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="429" mass="47485" checksum="D52DAF6EC43E896F" modified="2019-05-08" version="1">MSGGVYGGDEVGALVFDIGSYTVRAGYAGEDCPKVDFPTAIGVVLERDDGSTLMEIDGDKGKQGGPTYYIDTNALRVPRENMEAISPLKNGMIEDWDSFQAILDHTYKMHIKSEASLHPVLMSEAPWNTRAKREKLTELMFEHYNIPAFFLCKTAVLTAFANGRSTGLILDSGATHTTAIPVHDGYVLQQGIVKSPLAGDFITMQCRELFQEMNIELIPPYMIASKEAVREGSPANWKRKEKLPQVTRSWHNYMCNCVIQDFQASVLQVSDSTYDEQVAAQMPTVHYEFPNGYNCDFGAERLKIPEGLFDPSNVKGLSGNTMLGVSHVVTTSVGMCDIDIRPGLYGSVIVAGGNTLIQSFTDRLNRELSQKTPPSMRLKLIANNTTVERRFSSWIGGSILASLGTFQQMWISKQEYEEGGKQCVERKCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I804</accession>
    <name>A0A452I804_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bcl-2 Bcl-2 homology region 1-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Bcl-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I804"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023656"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026951.1">
        <property type="protein sequence ID" value="ENSGAGP00000023656.1"/>
        <property type="gene ID" value="ENSGAGG00000017332.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06845">
        <property type="entry name" value="Bcl-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020726">
        <property type="entry name" value="Bcl2_BH2_motif_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF47">
        <property type="entry name" value="BCL-2-LIKE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01862">
        <property type="entry name" value="BCL2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00337">
        <property type="entry name" value="BCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01258">
        <property type="entry name" value="BH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="8">
        <location>
            <begin position="4"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="146" mass="15638" checksum="6CD86FC8B57988D5" modified="2019-05-08" version="1">AETLRRAASELESRERPFFRACRPLALDERQEPAALLSRVAEQLEAEGGLNWGRVLALVVFAGSLAAALAEQGSREEAGRLAEVLASYLAEEKREWLEAHGGWDGFCHFFNGHGSQPTDQSSTISSAIMAAAGFGLAGLAVLLAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8L5</accession>
    <name>A0A452I8L5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000023999"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027332.1">
        <property type="protein sequence ID" value="ENSGAGP00000023999.1"/>
        <property type="gene ID" value="ENSGAGG00000017165.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030056">
        <property type="term" value="C:hemidesmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031581">
        <property type="term" value="P:hemidesmosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000350">
        <property type="entry name" value="Dystonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000392">
        <property type="entry name" value="Dystonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000074">
        <property type="entry name" value="dystonin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000077">
        <property type="entry name" value="dystonin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000093">
        <property type="entry name" value="dystonin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000114">
        <property type="entry name" value="dystonin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000121">
        <property type="entry name" value="dystonin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000122">
        <property type="entry name" value="dystonin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.920.20:FF:000002">
        <property type="entry name" value="dystonin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000085">
        <property type="entry name" value="dystonin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000094">
        <property type="entry name" value="dystonin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000098">
        <property type="entry name" value="dystonin isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000013">
        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000001">
        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000008">
        <property type="entry name" value="microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000012">
        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000014">
        <property type="entry name" value="microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000016">
        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000021">
        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000022">
        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000025">
        <property type="entry name" value="microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000031">
        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.20">
        <property type="entry name" value="Gas2-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003108">
        <property type="entry name" value="GAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036534">
        <property type="entry name" value="GAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169:SF24">
        <property type="entry name" value="DYSTONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02187">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SMART" id="SM01129">
        <property type="entry name" value="DELLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00243">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143575">
        <property type="entry name" value="GAS2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51460">
        <property type="entry name" value="GAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="3666"/>
            <end position="3701"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="3702"/>
            <end position="3737"/>
        </location>
    </feature>
    <feature type="domain" description="GAR" evidence="9">
        <location>
            <begin position="3742"/>
            <end position="3822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="3827"/>
            <end position="3848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="3870"/>
            <end position="3967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="3987"/>
            <end position="4078"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="810"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1027"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1241"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1491"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1673"/>
            <end position="1743"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="2663"/>
            <end position="2690"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="2779"/>
            <end position="2813"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="2915"/>
            <end position="2953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="3833"/>
            <end position="3844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="3881"/>
            <end position="3907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="3930"/>
            <end position="3949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="3997"/>
            <end position="4011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="4026"/>
            <end position="4043"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="4078" mass="463894" checksum="1E267D779F92EE26" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8U0</accession>
    <name>A0A452I8U0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 13">Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="13">Binds 1 heme group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the cytochrome c family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I8U0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024079"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027422.1">
        <property type="protein sequence ID" value="ENSGAGP00000024079.1"/>
        <property type="gene ID" value="ENSGAGG00000017618.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000001">
        <property type="entry name" value="Cytochrome c iso-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002327">
        <property type="entry name" value="Cyt_c_1A/1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11961">
        <property type="entry name" value="CYTOCHROME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00604">
        <property type="entry name" value="CYTCHRMECIAB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 13" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 11 13" id="KW-0349">Heme</keyword>
    <keyword evidence="10 11 13" id="KW-0408">Iron</keyword>
    <keyword evidence="7 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="14">
        <location>
            <begin position="2"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="105" mass="11691" checksum="0F425B0F1740A5C1" modified="2019-05-08" version="1">MGDAEKGKKLFVQKCSQCHTVEKGGPHKTGPNLWGMFSRKTGQAAGYSYSDSNKNKGVVWNENTLMEYLENPKKYIPGTKMIFAGIKKKNERADVIAYLKKATSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8Z8</accession>
    <name>A0A452I8Z8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I8Z8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024140"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027488.1">
        <property type="protein sequence ID" value="ENSGAGP00000024140.1"/>
        <property type="gene ID" value="ENSGAGG00000017655.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009615">
        <property type="term" value="P:response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053270">
        <property type="entry name" value="Fv1_restriction_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48195">
        <property type="entry name" value="FRIEND VIRUS SUSCEPTIBILITY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48195:SF1">
        <property type="entry name" value="RIKEN CDNA 2410002F23 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="290" mass="31413" checksum="F059B2CFD1400C73" modified="2019-05-08" version="1">VAKALILPTEIGDKQDRELQEIVKSFRQKPGEGVLSWLVRVWDNAGNTVLLLYYELRQLGVMAQDAQVRAEMSTLGPPAEGQPESPSLYRWLAEAVGAAYPSVGELEALVSPWKTISEGIQALRQLGMAWAVSTGGTEGPDDMLLGMAWAVSTGGTEGPDDMLVSKSTKAAFIRTAPDELKPSLLAVVMAADGRVGDLAATLMQLGTALAGAWPKGEIRVPRRVLWSDLLQAGVPREDIDRQPTVDLYKRWMQLPAAKRSMGPEGEKSVEQSKPAEPSAPPADWQLPRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9Q9</accession>
    <name>A0A452I9Q9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Glutaredoxin-2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer; active form. Homodimer; inactive form. The homodimer is probably linked by 1 2Fe-2S cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I9Q9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024272"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027629.1">
        <property type="protein sequence ID" value="ENSGAGP00000024272.1"/>
        <property type="gene ID" value="ENSGAGG00000017740.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000026">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46679">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46679:SF1">
        <property type="entry name" value="GLUTAREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12" id="KW-0318">Glutathionylation</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="17">
        <location>
            <begin position="45"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="144" mass="15749" checksum="E629A2CD6FB3CE04" modified="2019-05-08" version="1">MMALSGALRAAGSRLRMGNSPSTSMGLSNAATVNKIQETISDNCVVIFSKTTCGYCKMAKKLFQDMNVNYTAVELDMYENGSQFQDILHQMTGGRTVPRVFINGAFIGGATDTQRLHQEGKLLPLVHQCQMRRAKNSDLPCSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9X9</accession>
    <name>A0A452I9X9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Syntaxin-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein unc-18 homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein unc-18 homolog A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I9X9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024497"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027879.1">
        <property type="protein sequence ID" value="ENSGAGP00000024497.1"/>
        <property type="gene ID" value="ENSGAGG00000017896.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017075">
        <property type="term" value="F:syntaxin-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903305">
        <property type="term" value="P:regulation of regulated secretory pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.60:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2060:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.830.10:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="603" mass="68813" checksum="2E35C8FFBDFAB425" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ID81</accession>
    <name>A0A452ID81_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Troponin I, fast skeletal muscle</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Troponin I, fast-twitch isoform</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Binds to actin and tropomyosin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ID81"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025797"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029346.1">
        <property type="protein sequence ID" value="ENSGAGP00000025797.1"/>
        <property type="gene ID" value="ENSGAGG00000018830.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000002">
        <property type="entry name" value="troponin I, fast skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050875">
        <property type="entry name" value="Troponin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738:SF15">
        <property type="entry name" value="TROPONIN I, FAST SKELETAL MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="37"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="162" mass="18826" checksum="F10613A3D3641B48" modified="2019-05-08" version="1">MLQIAATLIEKETAAKEAEKANYLAEHCPPLSLPHSMQELQDLCRKLHAKIEVVDEERYDTEAKLQKTTKELEDLTQKLFDLRGKFKRPPLRRVRMSADAMLRALLGSKHKVCMDLRANLKQVKKEDTEKEKDLRDVGDWRKNIEEKSGMEGRKKMFETSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDX3</accession>
    <name>A0A452IDX3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDX3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025700"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029240.1">
        <property type="protein sequence ID" value="ENSGAGP00000025700.1"/>
        <property type="gene ID" value="ENSGAGG00000018764.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046975">
        <property type="term" value="F:histone H3K36 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010844">
        <property type="term" value="F:recombination hotspot binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010845">
        <property type="term" value="P:positive regulation of reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000029">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003017">
        <property type="entry name" value="Si:cabz01054396.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001772">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000506">
        <property type="entry name" value="Zinc finger protein 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001271">
        <property type="entry name" value="Zinc finger protein 282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000200">
        <property type="entry name" value="zinc finger protein 510 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF10">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE PRDM9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="388" mass="44432" checksum="DD8B0DFCAA4E7407" modified="2019-05-08" version="1">MAADTWLTSHEKNKEQDSQELGPHWTAAQRPEENIYHSAEHGLPSNGPDMTLAVHMPEKLTQHERDFGPLKASTVCQGAAVTERPYLCSECGKCFSRREHFVQHQCTHTGERSYVCPECGKGFTQQSNLTNHYRTHTGERAHVCPECGRGFIHQSTLTTHLRTHTGEKPYKCSVCAKCFSRLSTLLEHQRTHTGEKPYKCLECEKRFSRLSTLVEHRRTHTGEKPYKCAQCEKSFTRLTNLTVHQDTHAGERAYRCTQCGKSFAQKPYFLKHLRNHTKEKLYKCLECGKSFVCHSWLVRHQMIHTGERPYQCGECGKSFVQKEHLLKHQRVHTGEQPFQCSACAKSFRCQQSLRLHQCTHASQQGGTPATVGAEFKNQLLYLKMENTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IEF5</accession>
    <name>A0A452IEF5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RNA-splicing ligase RtcB homolog</fullName>
            <ecNumber evidence="10">6.5.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">3'-phosphate/5'-hydroxy nucleic acid ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RTCB</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10 13">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Catalytic component of the tRNA-splicing ligase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the RtcB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.8" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452IEF5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026213"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029810.1">
        <property type="protein sequence ID" value="ENSGAGP00000026213.1"/>
        <property type="gene ID" value="ENSGAGG00000019133.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170057">
        <property type="term" value="F:RNA ligase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1860.10:FF:000001">
        <property type="entry name" value="tRNA-splicing ligase RtcB homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="entry name" value="tRNA-splicing ligase RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03144">
        <property type="entry name" value="RtcB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027513">
        <property type="entry name" value="RtcB_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118:SF1">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="Hypothetical protein PH1602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01288">
        <property type="entry name" value="UPF0027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0819">tRNA processing</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="10 11">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <begin position="226"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <begin position="353"/>
            <end position="354"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <begin position="402"/>
            <end position="405"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <begin position="428"/>
            <end position="431"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="481" mass="52726" checksum="1003D8407F5109A6" modified="2019-05-08" version="1">MSRSYNDELQYLDKLNRTSWRIRKGFVPNMNVEGVFYVNEPLEKLMFEELRNACRGGGAGGFLPAMKQIGNVAALPGIIHRSIGLPDVHSGYGFAIGNMAAFDMSDLDAVVSPGGVGFDINCGVRLLRTNLDESDVQPVKEQLAQAMFDHIPVGVGSKGVIPMNAKDLEEALEMGVDWSLREGYAWAEDKEHCEEYGRMLQADPNKVSSRAKKRGLPQLGTLGAGNHYAEIQVVDDIYNEYAARKMGIDHKGQVCVMIHSGSRGLGHQVATDALVAMEKAMKRDKIIVNDRQLACARIASPEGQDYLKGMAAAGNYAWVNRSSMTFLTRQAFAKVFNTTPDDLDLHVIYDVSHNIAKVEQHVVDGKERTLLVHRKGSTRAFPPHHPLIAVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMTETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLADMGIAIRVASPKLVMEEVSFKLLIGCVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IG14</accession>
    <name>A0A452IG14_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IG14"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026691"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030335.1">
        <property type="protein sequence ID" value="ENSGAGP00000026691.1"/>
        <property type="gene ID" value="ENSGAGG00000019438.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050637">
        <property type="entry name" value="NLRP_innate_immun_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45690">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45690:SF19">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="103"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="622" mass="72541" checksum="F2F5EC3DBF0F02C3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IG44</accession>
    <name>A0A452IG44_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Calpain small subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IG44"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026442"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030056.1">
        <property type="protein sequence ID" value="ENSGAGP00000026442.1"/>
        <property type="gene ID" value="ENSGAGG00000019265.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110158">
        <property type="term" value="C:calpain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46735:SF3">
        <property type="entry name" value="CALPAIN SMALL SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46735">
        <property type="entry name" value="CALPAIN, SMALL SUBUNIT 1 A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11317" checksum="55924DD5959D6546" modified="2019-05-08" version="1">EAAGKEPLRGGIGGGWVGTHKGFRLNEQLYQMIVRRYSDENSHMDFDNFISCLVRLDAMFRAFKSLDKDGDGQIRVNIQEVSEAGGWPPPPAFSFLLSSKGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGL9</accession>
    <name>A0A452IGL9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IGL9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026619"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030257.1">
        <property type="protein sequence ID" value="ENSGAGP00000026619.1"/>
        <property type="gene ID" value="ENSGAGG00000019389.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="106" mass="10634" checksum="5F5CF5744BCF675D" modified="2019-05-08" version="1">MGNTVTCCVSPDTSPKLGRARAGGGGGAAPGSERRADAYQVAAAAAEAESGDSDPGGGPHLQHISDREFPDGERRSERPEGEGDRTGPGGCSEGPGRLLPSQSPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IH24</accession>
    <name>A0A452IH24_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">R3H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IH24"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027072"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030762.1">
        <property type="protein sequence ID" value="ENSGAGP00000027072.1"/>
        <property type="gene ID" value="ENSGAGG00000019698.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000030765.1">
        <property type="protein sequence ID" value="ENSGAGP00000027075.1"/>
        <property type="gene ID" value="ENSGAGG00000019698.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02325">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025952">
        <property type="entry name" value="R3H-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039629">
        <property type="entry name" value="R3HDM4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32019">
        <property type="entry name" value="R3H DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32019:SF2">
        <property type="entry name" value="R3H DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13902">
        <property type="entry name" value="R3H-assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="1">
        <location>
            <begin position="181"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="262" mass="30416" checksum="880B1BDE38E350B9" modified="2019-05-08" version="1">MVVLTGREAAGGQTLEEPYLRIEDCLPPVGSSPSKRVSPSKRKQYYINQAIRNSDLIPKAKGRKSLQRLENTRYLMTLLERDECGNDDGELTHSAAPSIFTEACNNEMYMEIWNDFMNRSGEEQERVLLYLEEEAKKQHKRKLPVKAEDKWKEHPAYTPKECFQRISRRLRTTLRRGRIPMGTLECLEEELLAFFSVTPHSVYTAMMDNSFERLLLHALCQYMDLVSASSDFEGKRQMKVSNKHRDFLPPELLLSAYLEQMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHS0</accession>
    <name>A0A452IHS0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc-finger CCCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IHS0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027104"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030796.1">
        <property type="protein sequence ID" value="ENSGAGP00000027104.1"/>
        <property type="gene ID" value="ENSGAGG00000019714.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040943">
        <property type="entry name" value="DUF5571"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041686">
        <property type="entry name" value="Znf-CCCH_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15725:SF1">
        <property type="entry name" value="RIKEN CDNA 1700017N19 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15725">
        <property type="entry name" value="ZN-FINGER, C-X8-C-X5-C-X3-H TYPE-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17732">
        <property type="entry name" value="DUF5571"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15663">
        <property type="entry name" value="zf-CCCH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc-finger CCCH" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="353" mass="39518" checksum="5538BB00C781D0EC" modified="2019-05-08" version="1">MEIQQKYTRISCFWETQPLGCVRISCVFHHSKPRNINGLFLPPSSNATLQKEVQEGTLHPAQTQEPLKGQESILRPIHPPLIINISLEEEEDEEEEENYASYLLTKTPADIEEEKAIKEMCYKSGEYYRIQTPQENQSAKIMSSTLDNELLKTMETGRDLQEGDGVTVPTKFNIIERQGEVTASLDGNTRADLGAFENGGGDCYVPQRSIFVGGKQSEILTGEKELITSRRSDVKATSHTEPVKKRHFKGVKKKKWISEEPKNSFSMFAGKAMHASNSKGKANCQQNYQNKDAENTYVPSQRATGRSISLNSPEPGRSTNMTYSNVSVTKESKLNPSTGELLITQVSLPRRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJ32</accession>
    <name>A0A452IJ32_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L27a</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJ32"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027810"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031595.1">
        <property type="protein sequence ID" value="ENSGAGP00000027810.1"/>
        <property type="gene ID" value="ENSGAGG00000020213.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027386">
        <property type="entry name" value="Rbsml_uL15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="64" mass="7158" checksum="F8BB96304DB93BB7" modified="2019-05-08" version="1">MGGSPRSGEGPSRLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYGHFEPFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJA2</accession>
    <name>A0A452IJA2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IJA2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027881"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031670.1">
        <property type="protein sequence ID" value="ENSGAGP00000027881.1"/>
        <property type="gene ID" value="ENSGAGG00000020259.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF81">
        <property type="entry name" value="GRANZYME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="109" mass="12014" checksum="B5B3C6DCEB7F4AFF" modified="2019-05-08" version="1">RAPFIMWLFFEPGLSPGEIIGGWEAKPHSRSYMAHLAIQCKKKTYICGGFLVSEKFVLMAGDSGGPLVCGKTAQSVVTWGPVNGSPPRVYAKVSTFIPWIQAMMRRLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKB9</accession>
    <name>A0A452IKB9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase subunit g</fullName>
            <shortName evidence="10">ATPase subunit g</shortName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATPase g subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IKB9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028335"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032192.1">
        <property type="protein sequence ID" value="ENSGAGP00000028335.1"/>
        <property type="gene ID" value="ENSGAGG00000020573.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016702">
        <property type="entry name" value="ATP5MG_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006808">
        <property type="entry name" value="ATP_synth_F0_gsu_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12386">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04718">
        <property type="entry name" value="ATP-synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017835">
        <property type="entry name" value="ATP-synth_g_mitoch_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4 10" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR017835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="107" mass="11755" checksum="A4E70EEE45C9C6BB" modified="2019-05-08" version="1">MAQAAQRLVQRVATKGPQLVSAAVTYSRPRLATFWHYAKVELVPPTPAEIPKAIDSMKAMVKSFQSGHLAQLTVKEALRNGLVATEVLMWFYIGEIIGRGSLIGYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IML7</accession>
    <name>A0A452IML7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the interleukin-1 receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IML7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029114"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033070.1">
        <property type="protein sequence ID" value="ENSGAGP00000029114.1"/>
        <property type="gene ID" value="ENSGAGG00000021065.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045920">
        <property type="term" value="P:negative regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000188">
        <property type="entry name" value="Interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF22">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR ACCESSORY PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="150" mass="17682" checksum="B79B50B3A17131EA" modified="2019-05-08" version="1">FMYLVMRLKMCPHGLKQVQAKLSREHQKGTVMCHYLLKNEKTKINTITILFEKIQSVEPLFLFVLYYRNSTYCMKVSISLTVGENDTGLCYNSKMKYFEKAELSKSKEISCPDIEDFLIPYREPEILWYKACPNLERIWHSAIQGKIILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IP50</accession>
    <name>A0A452IP50_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IP50"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029687"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033708.1">
        <property type="protein sequence ID" value="ENSGAGP00000029687.1"/>
        <property type="gene ID" value="ENSGAGG00000021428.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="20"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="207" mass="24321" checksum="2B8531477AB9ED3E" modified="2019-05-08" version="1">IFPAKNKAFRVQNKVFHKDNQALRAENQALSEDNKILQERKMDYFEEKKVLEKQEKALRKQNKALWEEIKALQEQEIAFQMEEKALQEETVALWEENKAFQVQIKALQKDEIALQEEKKALQKQGKALQEEKIALREENRALHEENKALQNEKKALDEENKPKCYLTSCVLGFHNVPSAYPLLFAYRWNSGAESWEFAVKRLNDTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPQ5</accession>
    <name>A0A452IPQ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">26S proteasome non-ATPase regulatory subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">26S proteasome regulatory subunit RPN7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD6, a base containing 6 ATPases and few additional components.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the proteasome subunit S10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IPQ5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029930"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033989.1">
        <property type="protein sequence ID" value="ENSGAGP00000029930.1"/>
        <property type="gene ID" value="ENSGAGG00000021605.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005838">
        <property type="term" value="C:proteasome regulatory particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000004">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049549">
        <property type="entry name" value="RPN7_PSMD6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21154">
        <property type="entry name" value="RPN7_PSMD6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="169"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="43"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00075096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="365" mass="43002" checksum="D5C6B93D1EBBCB8B" modified="2019-05-08" version="1">MQRLNPGQRELSLLHNWRMSGVGEDMAPYYEALCKPLDWQMDMELLNKMKKANEEELKRLDNELEDAEKILGESEIRDAMMAKAEYLCRIGDKEGALTAFRKTYDKTVALGHRLDIVFYLLRIGLFYMDNDLITRNIEKAKSLIEEGGDWDRRNRLKVYQGLYCVAIRDFKQAAELFLDTVSTFTSYELMDYKTFVTYTVYVSMIALDRPDLREKVIKGAEILEVLHSLPTVRQYLFSLYECRYSVFFQSLAIVEQEMKKDWLFAPHYRYYVREMRIHAYSQLLESYRSLTLGYMAEAFGVGVEFIDQELSRFIAAGRLHCKIDKVNEIVETNRPDSKNWQYQETIKKGDLLLNRVQKLSRVINM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQN5</accession>
    <name>A0A452IQN5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000030280"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034367.1">
        <property type="protein sequence ID" value="ENSGAGP00000030280.1"/>
        <property type="gene ID" value="ENSGAGG00000021821.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000003">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000021">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000009">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000010">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF50">
        <property type="entry name" value="MYOSIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 10" id="KW-0518">Myosin</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="13">
        <location>
            <begin position="35"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="12">
        <location>
            <begin position="88"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="10">
        <location>
            <begin position="649"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1175"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1175"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1190"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="181"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1939" mass="223875" checksum="0D7A58DACEC3639C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ISB0</accession>
    <name>A0A452ISB0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030752"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034879.1">
        <property type="protein sequence ID" value="ENSGAGP00000030752.1"/>
        <property type="gene ID" value="ENSGAGG00000022110.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000051">
        <property type="term" value="P:negative regulation of non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16561">
        <property type="entry name" value="RING-HC_RNF213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000488">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000491">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000804">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031248">
        <property type="entry name" value="RNF213"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046439">
        <property type="entry name" value="ZF_RZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22605:SF16">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22605">
        <property type="entry name" value="RZ-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20173">
        <property type="entry name" value="ZnF_RZ-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51981">
        <property type="entry name" value="ZF_RZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <keyword evidence="13 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="24">
        <location>
            <begin position="3629"/>
            <end position="3668"/>
        </location>
    </feature>
    <feature type="domain" description="RZ-type" evidence="25">
        <location>
            <begin position="4112"/>
            <end position="4191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="188"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="80"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="4842" mass="553997" checksum="DF7754A439C7D517" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ISJ4</accession>
    <name>A0A452ISJ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Adhesion G-protein coupled receptor D1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">G-protein coupled receptor 133</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Adhesion G-protein coupled receptor (aGPCR) for androgen hormone 5alpha-dihydrotestosterone (5alpha-DHT), also named 17beta-hydroxy-5alpha-androstan-3-one, the most potent hormone among androgens. Also activated by methenolone drug. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. ADGRD1 is coupled to G(s) G proteins and mediates activation of adenylate cyclase activity. Acts as a 5alpha-DHT receptor in muscle cells, thereby increasing intracellular cyclic AMP (cAMP) levels and enhancing muscle strength.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment and the membrane-bound C-terminal fragment predominantly remain associated and non-covalently linked. Interacts with ESYT1; interaction takes place in absence of cytosolic calcium and inhibits the G protein-coupled receptor activity of ADGRD1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ISJ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030992"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035140.1">
        <property type="protein sequence ID" value="ENSGAGP00000030992.1"/>
        <property type="gene ID" value="ENSGAGG00000022270.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014819">
        <property type="term" value="P:regulation of skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000073">
        <property type="entry name" value="Adhesion G-protein coupled receptor D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000104">
        <property type="entry name" value="adhesion G-protein coupled receptor D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.50:FF:000008">
        <property type="entry name" value="Adhesion G-protein coupled receptor D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF216">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="584"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="619"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="649"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="688"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="727"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="19">
        <location>
            <begin position="94"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="17">
        <location>
            <begin position="399"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="18">
        <location>
            <begin position="581"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="815" mass="91075" checksum="C19655A13446BDB9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITF4</accession>
    <name>A0A452ITF4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein sorting-associated protein 54</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. EIPR1 interacts with GARP complex and mediates its recruitment to the trans-Golgi network. Interacts with VPS51 in an EIPR1-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS54 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ITF4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031332"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035527.1">
        <property type="protein sequence ID" value="ENSGAGP00000031323.1"/>
        <property type="gene ID" value="ENSGAGG00000022507.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000035540.1">
        <property type="protein sequence ID" value="ENSGAGP00000031332.1"/>
        <property type="gene ID" value="ENSGAGG00000022507.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.130:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039745">
        <property type="entry name" value="Vps54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012501">
        <property type="entry name" value="Vps54_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019515">
        <property type="entry name" value="VPS54_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07928">
        <property type="entry name" value="Vps54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10475">
        <property type="entry name" value="Vps54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="943"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 N-terminal" evidence="14">
        <location>
            <begin position="207"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 C-terminal" evidence="13">
        <location>
            <begin position="736"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="529"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="559"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="978" mass="110874" checksum="8D18BF87D80DDD24" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITT2</accession>
    <name>A0A452ITT2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452ITT2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031519"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035745.1">
        <property type="protein sequence ID" value="ENSGAGP00000031519.1"/>
        <property type="gene ID" value="ENSGAGG00000022639.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000035746.1">
        <property type="protein sequence ID" value="ENSGAGP00000031520.1"/>
        <property type="gene ID" value="ENSGAGG00000022639.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000035748.1">
        <property type="protein sequence ID" value="ENSGAGP00000031522.1"/>
        <property type="gene ID" value="ENSGAGG00000022639.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07830">
        <property type="entry name" value="STKc_MAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000104">
        <property type="entry name" value="serine/threonine-protein kinase MAK isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000071">
        <property type="entry name" value="serine/threonine-protein kinase MAK isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="4"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="22">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="599" mass="67658" checksum="CBE6F4023DC77A36" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUD8</accession>
    <name>A0A452IUD8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IUD8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031616"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035856.1">
        <property type="protein sequence ID" value="ENSGAGP00000031616.1"/>
        <property type="gene ID" value="ENSGAGG00000022707.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000012">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="11"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="146" mass="16514" checksum="70F3C4328B4F56B8" modified="2019-05-08" version="1">PARSAGRVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQCYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IY04</accession>
    <name>A0A452IY04_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IY04"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032960"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037356.1">
        <property type="protein sequence ID" value="ENSGAGP00000032960.1"/>
        <property type="gene ID" value="ENSGAGG00000023532.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021475">
        <property type="entry name" value="Pants/Emi1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11326">
        <property type="entry name" value="PANTS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="93" mass="11116" checksum="0E60E08CF2581CE7" modified="2019-05-08" version="1">MTDRGMWRPPQTCEDYWSEWKHSKGIRNLFHKYYTYGEAPFCEQWKIDYGNCRKWEKNASPIKMCVALPHILILMVIPAMPPPRHVTYICIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYY7</accession>
    <name>A0A452IYY7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydroxyacid-oxoacid transhydrogenase, mitochondrial</fullName>
            <ecNumber evidence="4">1.1.99.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alcohol dehydrogenase iron-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-3-hydroxybutanoate + 2-oxoglutarate = (R)-2-hydroxyglutarate + acetoacetate</text>
            <dbReference type="Rhea" id="RHEA:23048"/>
            <dbReference type="ChEBI" id="CHEBI:11047"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="EC" id="1.1.99.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>4-hydroxybutanoate + 2-oxoglutarate = (R)-2-hydroxyglutarate + succinate semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:24734"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16724"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="EC" id="1.1.99.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.24" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452IYY7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033274"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037702.1">
        <property type="protein sequence ID" value="ENSGAGP00000033274.1"/>
        <property type="gene ID" value="ENSGAGG00000023719.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047988">
        <property type="term" value="F:hydroxyacid-oxoacid transhydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08190">
        <property type="entry name" value="HOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1090.10:FF:000003">
        <property type="entry name" value="Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000010">
        <property type="entry name" value="Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056798">
        <property type="entry name" value="ADH_Fe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042157">
        <property type="entry name" value="HOT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496:SF83">
        <property type="entry name" value="HYDROXYACID-OXOACID TRANSHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25137">
        <property type="entry name" value="ADH_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA" evidence="11">
        <location>
            <begin position="51"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-containing alcohol dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="272"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="440" mass="47683" checksum="56BABF4C2F322988" modified="2019-05-08" version="1">MAGGRERVAHLLRQLQRAACQCPAHSHTYSQAPEHAPLRRTDYAFEMAVSNIRYGEGVTKEVGMDLHNLGARKVCVMTDQNLCQLPPVKAVLNSLTKNGITFQMYDDVRVEPTDQSFMDAITFAKKGEFDAYVAVGGGSVIDTCKAANLYSSSPESDFLDYVNAPIGKGKSVTVPLKPFIAVPTTAGTGSETTGIAIFDYKELKVKTGIASRAIKPTLGMIDPLHTIHMPERVVANSGFDVLCHALEAYTALPYKMRSPCPSNPINRPAYQGSNPISDVWAVHALRIVAEYLKRAVRMPDDHEARANMHLASAFAGIGFGNAGVHLCHGMSYPISGLVKSYRAKDYNVDHPLVPHGLSVVLTSPAVFTFTAEMCPERHLEAAEILGADIRTAKIKDAGLILADTLRKFLFDLNVDDGLAAIGYSKADIPALVKGTLPQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J003</accession>
    <name>A0A452J003_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DOMON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000033645"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038110.1">
        <property type="protein sequence ID" value="ENSGAGP00000033645.1"/>
        <property type="gene ID" value="ENSGAGG00000023950.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900449">
        <property type="term" value="P:regulation of glutamate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09628">
        <property type="entry name" value="DOMON_SDR_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042789">
        <property type="entry name" value="FRRS1L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46902">
        <property type="entry name" value="DOMON DOMAIN-CONTAINING PROTEIN FRRS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46902:SF1">
        <property type="entry name" value="DOMON DOMAIN-CONTAINING PROTEIN FRRS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="3">
        <location>
            <begin position="161"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="335" mass="36962" checksum="685ADB86DF7CC31A" modified="2019-05-08" version="1">MGALCTGHGEREAWEPPIPAGGRTAPRSPLPLPSQDAASSQRRRRALLHASLGASPGCGAAMSPLRWGAWLLLLAGPAASTASPADESGGAXGDEPQRQHHDSSYGTFASEFYDLRYLSEEGYPFPTAPPVDPFAKIKVDDCGKTKGCFRYGKPGCNAETCDYFLSYRRIGADVEFELSADTDGWVAVGFSSDKKMGGDDVMACVHDDNGRVRIQHFYNVGQWAKEIQRNPARDEEGIFENNRVTCRFKRPVYVPRDETIVDLHLSWYYLFAWGPAIQGSITRHDIDSPPVSERVVSIYKYEDIFMPSAAYQTFSSPFCLLLIVALTFYLLMGTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0X0</accession>
    <name>A0A452J0X0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LisH domain and HEAT repeat-containing protein KIAA1468</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000034004"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038504.1">
        <property type="protein sequence ID" value="ENSGAGP00000034004.1"/>
        <property type="gene ID" value="ENSGAGG00000023990.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032367">
        <property type="term" value="P:intracellular cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000080">
        <property type="entry name" value="lisH domain and HEAT repeat-containing protein KIAA1468 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000218">
        <property type="entry name" value="lisH domain and HEAT repeat-containing protein KIAA1468 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040362">
        <property type="entry name" value="RELCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32059">
        <property type="entry name" value="RAB11-BINDING PROTEIN RELCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32059:SF0">
        <property type="entry name" value="RAB11-BINDING PROTEIN RELCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="HEAT" evidence="10">
        <location>
            <begin position="995"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="399"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="182"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="343"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="405"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00084053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1205" mass="133719" checksum="AD6FC294968A2CEF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0X9</accession>
    <name>A0A452J0X9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Roadblock/LAMTOR2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GAMAD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J0X9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034014"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038514.1">
        <property type="protein sequence ID" value="ENSGAGP00000034014.1"/>
        <property type="gene ID" value="ENSGAGG00000024173.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000002">
        <property type="entry name" value="Dynein light chain roadblock"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10779">
        <property type="entry name" value="DYNEIN LIGHT CHAIN ROADBLOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Roadblock/LAMTOR2" evidence="9">
        <location>
            <begin position="68"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="160" mass="17610" checksum="EDFA23C107B5D118" modified="2019-05-08" version="1">MVAARSSVGQGTYWPLLPAAPIGYWEPQSTEPAWQVNKPAQPARGFPHTSGGTSLGTTALTAWLQAEVEETLKRIQSQKGVQGIIVVNSEGIPIKSTVDNSTTVQYAGLMHSFIMKARSTVRDIDPQNDLTFLRIRSKKNEIMIAPDKDYFLIVIQNPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1N6</accession>
    <name>A0A452J1N6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="2">2.3.1.234</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452J1N6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034311"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038852.1">
        <property type="protein sequence ID" value="ENSGAGP00000034311.1"/>
        <property type="gene ID" value="ENSGAGG00000024386.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd24134">
        <property type="entry name" value="ASKHA_NBD_OSGEPL1_QRI7_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000106">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="11">
        <location>
            <begin position="74"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="437" mass="47662" checksum="4004A2E14E884297" modified="2019-05-08" version="1">MFWVQFFSRHASVQKILAISSVTRCTSKSVKHGQSAWLRSIFFNSVKYYPTKLVLGIETSCDDTAAAVVDDTGKILGEALHSQNEVHLKTGGIIPPVAQQLHRENIERIVKETLCVSGISLDELSAVATTVKPGLALSLGVGLEYSLKLVDKYKKPFIPIHHMEAHALTIRLINQVEFPFLVLLISGGHCILVVAQGVSDFLLLGQSVDIAPGDMLDKVARRLSLTKHPECCRMSGGKAIEHLAQQGNRLHYELGAPMRHHPNCNFSFSGLRSKATRIIMQKEKEEGLQEGHLLSCVTDIAAAVQHAVALHIAQRTHRAILFCIKSGILSQTNATLVVSGGVASNQYIRRALQIVTDATNFNLLCPPPRLCTDNGVMIAWNGIERMRAGSGVLHSTDGIRYEPKASLGIDISERVGEVAIKVPSLEVSLNELSAINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J242</accession>
    <name>A0A452J242_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J242"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034466"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039022.1">
        <property type="protein sequence ID" value="ENSGAGP00000034466.1"/>
        <property type="gene ID" value="ENSGAGG00000024490.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005903">
        <property type="term" value="C:brush border"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070837">
        <property type="term" value="P:dehydroascorbic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17431">
        <property type="entry name" value="MFS_GLUT_Class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF27">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="27"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="506" mass="55113" checksum="B6667B31BE966E66" modified="2025-10-08" version="2">MEGSSKSCYSWPQAFLSAYPPYTPFTGFLAVIEAHYGRVLGIAPIDRSAIDFTDTNGSMIFPDLEELDVGKSTLTLYWSLSVSIFAIGGMISSFTVGWVGDKLGRVKAMLAVNVLSIIGNLLMGLAKFGPSHILIIAGRAITGLYCGLSSGLVPMYVGEISPTALRGALGTLHQLAIVTGILLSQVLGLDFLLGNDDMWPLLLGLSGAAALLQFFLLLLCPESPRYLYIKLGKVEEAKKSLKRLRGDCDPTKEITEMEKEKQEAASEKKVSIRKLFSSSSYRQPIIVALMVQISQQFSGINAIFYYSTNIFVRAGVDQPVYATIGVGVVNTIFTIISVFLVERTGRRSLFIAGLIGMLASAVAMTVGLVLLGTFAWMSYVSMVAIFLFVIFFEVGPGPIPWFIVAELFSQGPRPAAIAIAGFCNWTCNFIIGMCFQYIADLCGPYVFTIFAVLLLGFILFAHFKVPETKGKSFEEISAAFRRKRRSAIKGHKAATELEDLRGSEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4S9</accession>
    <name>A0A452J4S9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the Crumbs protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000035486"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040190.1">
        <property type="protein sequence ID" value="ENSGAGP00000035486.1"/>
        <property type="gene ID" value="ENSGAGG00000025228.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120025">
        <property type="term" value="C:plasma membrane bounded cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000348">
        <property type="entry name" value="Crumbs 1, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000081">
        <property type="entry name" value="Crumbs 1, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000208">
        <property type="entry name" value="Crumbs 2, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000130">
        <property type="entry name" value="Crumbs 2, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000039">
        <property type="entry name" value="Crumbs cell polarity complex component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000400">
        <property type="entry name" value="Crumbs cell polarity complex component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000413">
        <property type="entry name" value="Crumbs cell polarity complex component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000055">
        <property type="entry name" value="Crumbs cell polarity complex component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000282">
        <property type="entry name" value="Crumbs cell polarity complex component 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000123">
        <property type="entry name" value="Crumbs homolog 1 (Drosophila)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000247">
        <property type="entry name" value="Delta/notch like EGF repeat containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000066">
        <property type="entry name" value="FAT atypical cadherin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000279">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000031">
        <property type="entry name" value="neurogenic locus notch homolog protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000327">
        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF4">
        <property type="entry name" value="UNINFLATABLE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 15" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019451729" evidence="17">
        <location>
            <begin position="28"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1343"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="32"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="109"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="147"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="185"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="223"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="261"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="300"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="338"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="396"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="440"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="18">
        <location>
            <begin position="484"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="670"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="18">
        <location>
            <begin position="712"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="886"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="18">
        <location>
            <begin position="949"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="1138"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="1176"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="1213"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="1254"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="1296"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="384"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="696"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="912"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="1164"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="1180"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="1201"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="1239"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="1284"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="1322"/>
            <end position="1331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1405" mass="155049" checksum="2D968691D8343D9F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J6B8</accession>
    <name>A0A452J6B8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Gamma-aminobutyric acid type A receptor subunit alpha6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J6B8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036021"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040775.1">
        <property type="protein sequence ID" value="ENSGAGP00000036021.1"/>
        <property type="gene ID" value="ENSGAGG00000025536.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19039">
        <property type="entry name" value="LGIC_ECD_GABAAR_A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19052">
        <property type="entry name" value="LGIC_TM_GABAAR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000001">
        <property type="entry name" value="Gamma-aminobutyric acid A receptor subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000002">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit alpha-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001390">
        <property type="entry name" value="GABAAa_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005436">
        <property type="entry name" value="GABBAa6_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047024">
        <property type="entry name" value="Gabra-1-6_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01079">
        <property type="entry name" value="GABAARALPHA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01619">
        <property type="entry name" value="GABAARALPHA6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="20">
        <location>
            <begin position="28"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="21">
        <location>
            <begin position="242"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="360"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="360"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="396"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="51576" checksum="6C01FAFA8A4BAC26" modified="2019-05-08" version="1">MAWLFSWIVDKALGSQTDEGKLYSENITRILDKLLDGYDNRLRPGFGGAVTEVKTDIYVTSFGPVSDVEMEYTMDVFFRQTWTDERLKFSGPTEILRLNNLMVSKIWTPDTFFRNGKKSIAHNMTTPNKLFRIMQNGTILYTMRLTINADCPMRLVNFPMDGHACPLKFGSYAYPKSEIIYTWKKGPLYSVEVPQESSSLLQYDLIGQTVSSETIKSNTGEYVIMTVYFHLQRKMGYFMIQIYTPCIMTVILSQVSFWINKESVPARTVFGITTVLTMTTLSISARHSLPKVSYATAMDWFIAVCFAFVFSALIEFAAVNYFTNLQTQRAMRKAARAAALSAALSAATVPAEDEIVSHSDSNCNLKKRMNSTTSQTDQSPEASIMSNSASQGQPTSIPPPVPPLPPPPPAIGGTSKIDQYSRILFPVAFAGFNLVYWVVYLSKDTMEVSTSLLSSLQHGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J6J5</accession>
    <name>A0A452J6J5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CCR4-NOT transcription complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">CCR4-associated factor 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT3. Interacts with NCOR1, NCOR2. HDAC3 and GPS2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J6J5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036107"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040874.1">
        <property type="protein sequence ID" value="ENSGAGP00000036107.1"/>
        <property type="gene ID" value="ENSGAGG00000025593.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000036">
        <property type="term" value="P:regulation of stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1020:FF:000001">
        <property type="entry name" value="Putative CCR4-NOT transcription complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="17">
        <location>
            <begin position="404"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="103"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="283"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="307"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="328"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="547" mass="60516" checksum="11F4B3B4466B7E57" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J6L5</accession>
    <name>A0A452J6L5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J6L5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035990"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040742.1">
        <property type="protein sequence ID" value="ENSGAGP00000035990.1"/>
        <property type="gene ID" value="ENSGAGG00000025522.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="82"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="341" mass="38907" checksum="1A1178C6A51CF9BC" modified="2019-05-08" version="1">MAQYLNKYFASVFNKANDELRDNGRMTNGNEDMEVHITTSEVEAKLEQLNGTKSEGPDNLHPRILKELEHEIASPLARIFNESVNSEVVPYDWRIANIVPIFKKEKKSDPSNYRPVSLTSVVCKVLEKILKEKVVKDIEVNGNWDKLQHGFTKGRSCQTNLISFFEKVTDYLDKGNAVDLIYLDFSKAFDTVPHGELLVKLEKMGINMKIERWIRNWLKGRLQRVVLKGELSGWKEVTSGVPQGSVLGPILFNLFITDLGTKSGNVLIKFADDTKLGGIANTEKDRDIIQEDLDDLVNWSNSNRMKFNSEKCKVMHLGINNKNFRYTLGTHQLEATEEEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J766</accession>
    <name>A0A452J766_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Synaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000036214"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040995.1">
        <property type="protein sequence ID" value="ENSGAGP00000036214.1"/>
        <property type="gene ID" value="ENSGAGG00000025654.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099151">
        <property type="term" value="P:regulation of postsynaptic density assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905606">
        <property type="term" value="P:regulation of presynapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099560">
        <property type="term" value="P:synaptic membrane adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000012">
        <property type="entry name" value="Leucine rich repeat containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000076">
        <property type="entry name" value="Leucine-rich repeat and Ig domain-containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF102">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019548027" description="Ig-like domain-containing protein" evidence="15">
        <location>
            <begin position="39"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="352"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="666" mass="74492" checksum="06AF5FDFF671563E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7P1</accession>
    <name>A0A452J7P1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J7P1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036533"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041371.1">
        <property type="protein sequence ID" value="ENSGAGP00000036533.1"/>
        <property type="gene ID" value="ENSGAGG00000025904.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019375">
        <property type="entry name" value="Ribosomal_bS1m"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13447">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13447:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10246">
        <property type="entry name" value="MRP-S35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="171" mass="18740" checksum="64364EB2F4AFDD95" modified="2019-05-08" version="1">MARPSWSRAASRSRAWQLLGAARRGSSYGTGDSLGPGVSPPEPEKRPSSLTSALGAGSRQVPSKNVESFASMLRRSPLVQMGPAKDKIVLGQIFHIVEDDLYIDFGGKFHCVCKRPEVDGEKYQKGSRVRLRLVDLELTSRFLGGKTDTTLLEADAVLLGLLESREIRQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGC9</accession>
    <name>A0A452GGC9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GGC9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000603"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000677.1">
        <property type="protein sequence ID" value="ENSGAGP00000000603.1"/>
        <property type="gene ID" value="ENSGAGG00000000494.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18450">
        <property type="entry name" value="BACK_KLHL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18240">
        <property type="entry name" value="BTB_POZ_KLHL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000041">
        <property type="entry name" value="kelch-like protein 10 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030608">
        <property type="entry name" value="KLHL10_BTB/POZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00501">
        <property type="entry name" value="KELCHREPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="37"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="606" mass="68690" checksum="61340BBE05C3284E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GH29</accession>
    <name>A0A452GH29_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Signal peptidase complex catalytic subunit SEC11</fullName>
            <ecNumber evidence="13">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the signal peptidase complex paralog A (SPC-A) composed of a catalytic subunit SEC11A and three accessory subunits SPCS1, SPCS2 and SPCS3. Within the complex, interacts with SPCS2 and SPCS3. The complex induces a local thinning of the ER membrane which is used to measure the length of the signal peptide (SP) h-region of protein substrates. This ensures the selectivity of the complex towards h-regions shorter than 18-20 amino acids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase S26B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A452GH29"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000907"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001024.1">
        <property type="protein sequence ID" value="ENSGAGP00000000904.1"/>
        <property type="gene ID" value="ENSGAGG00000000749.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000001027.1">
        <property type="protein sequence ID" value="ENSGAGP00000000907.1"/>
        <property type="gene ID" value="ENSGAGG00000000749.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009003">
        <property type="term" value="F:signal peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000003">
        <property type="entry name" value="Signal peptidase complex catalytic subunit SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019756">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF28">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00501">
        <property type="entry name" value="SPASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S24/S26A/S26B/S26C" evidence="14">
        <location>
            <begin position="36"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="179" mass="20603" checksum="7C414C6B6E8B16E6" modified="2019-05-08" version="1">MLSLDFLDDVRRMNKRQLYYQVLNFGMIVSSALMIWKGLMVVTGSESPIVVVLSGSMEPAFHRGDLLFLTNRIEDPIRVGEIVVFRIEGREIPIVHRVLKIHEKQNGDIKFLTKGDNNAVDDRGLYKQGQHWLEKKDVVGRARGFVPYIGIVTILMNDYPKFKYAVLFLLGLFVLVHRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHB2</accession>
    <name>A0A452GHB2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AGRL2-4 GAIN subdomain A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GHB2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000997"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001126.1">
        <property type="protein sequence ID" value="ENSGAGP00000000997.1"/>
        <property type="gene ID" value="ENSGAGG00000000809.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032471">
        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16489">
        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AGRL2-4 GAIN subdomain A" evidence="2">
        <location>
            <begin position="43"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="144" mass="15828" checksum="EED0D42E7C64A3C4" modified="2019-05-08" version="1">MRAGLQRQQDLSHEASPQPRPHSVWDSPAFLVPGPEPPRPRSNLLRVGSALLDPSNKRHWELIQQTEGGTAWLLKRYQDYASTLGQNLRQTYLNPFTLITPNIGEGVVAAWGRGRCMGVWLSRGGHGHCMGGLSLSGPSCAHVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJT4</accession>
    <name>A0A452GJT4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Peptidase metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="14">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GJT4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001858"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002112.1">
        <property type="protein sequence ID" value="ENSGAGP00000001858.1"/>
        <property type="gene ID" value="ENSGAGG00000001483.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000007">
        <property type="entry name" value="Collagenase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF267">
        <property type="entry name" value="MACROPHAGE METALLOELASTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="16">
        <location>
            <begin position="78"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cysteine switch" evidence="15">
        <location>
            <begin position="63"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="14">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="336" mass="37411" checksum="DB195451470D4BDD" modified="2019-05-08" version="1">AHSPRPCTPIFCLSPYLLPYPKLLNPRAIGKPITDKIREMQEFFGLKVTGELDSNTLEVMMQPRCGIPDVGQFRTFPGSPRWTKTDLKYRILNYTPDMEPADVDEAIEKAWNVWSSVTPLKFTRVYEGDADIMISFAAGFHGDFFSFDGVGGTLAHAYAPGEGIGGDAHFDEDEYWTKDLKGFNLFLVAAHEFGHSLGLHHSNVLGSLMYPAYLQREIRNYRLPQDDVEGIQTLYGKEETSLFFKVSLIQVTNNLMRMCNQEPYCVLPVGGVKGVRHLRPKSSVQGMAVTVVLALTFTQGLCSLLCPLGANHSQRDEKLVMPWLTLTPSARASSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GM21</accession>
    <name>A0A452GM21_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GM21"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002732"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003128.1">
        <property type="protein sequence ID" value="ENSGAGP00000002732.1"/>
        <property type="gene ID" value="ENSGAGG00000002191.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="37"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="116" mass="12605" checksum="FC0496E8224FC0F7" modified="2019-05-08" version="1">MGAGGSALLLRACQSVSAGDLRSSADPCCHVYARVQDHRTALHWACSAGHTDIVHFLLGLGVPVNDQDDVTSRNRQEIALMLLENRADPDAMDHLESTPLHRAATFLHVSQVKHKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GME7</accession>
    <name>A0A452GME7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000003013"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003453.1">
        <property type="protein sequence ID" value="ENSGAGP00000003013.1"/>
        <property type="gene ID" value="ENSGAGG00000002402.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16269">
        <property type="entry name" value="GBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1000.10">
        <property type="entry name" value="Guanylate-binding protein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037684">
        <property type="entry name" value="GBP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003191">
        <property type="entry name" value="Guanylate-bd/ATL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036543">
        <property type="entry name" value="Guanylate-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02841">
        <property type="entry name" value="GBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48340">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1 (GBP1), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GB1/RHD3-type G" evidence="8">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="437"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="575" mass="64671" checksum="9C7780D0CC5AD918" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNY4</accession>
    <name>A0A452GNY4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Synaptic cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GNY4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003598"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004144.1">
        <property type="protein sequence ID" value="ENSGAGP00000003598.1"/>
        <property type="gene ID" value="ENSGAGG00000002902.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000014">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor alpha4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000005">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor alpha4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000001">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022265467" evidence="18">
        <location>
            <begin position="24"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="239"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="598"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="20">
        <location>
            <begin position="32"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="21">
        <location>
            <begin position="245"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="499"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="499"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="624" mass="71317" checksum="58FB2D42F3A173E6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPV3</accession>
    <name>A0A452GPV3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">LIM domain kinase 2</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452GPV3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003937"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004583.1">
        <property type="protein sequence ID" value="ENSGAGP00000003937.1"/>
        <property type="gene ID" value="ENSGAGG00000003224.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09465">
        <property type="entry name" value="LIM2_LIMK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06754">
        <property type="entry name" value="PDZ_LIMK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14222">
        <property type="entry name" value="STKc_LIMK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000038">
        <property type="entry name" value="LIM domain kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000090">
        <property type="entry name" value="LIM domain kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000038">
        <property type="entry name" value="LIM domain kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000197">
        <property type="entry name" value="LIM domain kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000082">
        <property type="entry name" value="LIM domain kinase 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050940">
        <property type="entry name" value="Actin_reg-Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485:SF1">
        <property type="entry name" value="LIM DOMAIN KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="15 20" id="KW-0440">LIM domain</keyword>
    <keyword evidence="9 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13 20" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="24">
        <location>
            <begin position="12"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="24">
        <location>
            <begin position="73"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="25">
        <location>
            <begin position="154"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="333"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="283"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="644" mass="72634" checksum="CC6F8ADAC4A73186" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQA4</accession>
    <name>A0A452GQA4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Heparan sulfate 2-O-sulfotransferase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">2-O-sulfotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">HS 2-O-sulfotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Heparan sulfate 2-sulfotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQA4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004115"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004808.1">
        <property type="protein sequence ID" value="ENSGAGP00000004115.1"/>
        <property type="gene ID" value="ENSGAGG00000003387.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004394">
        <property type="term" value="F:heparan sulfate 2-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000534">
        <property type="entry name" value="Heparan sulfate 2-O-sulfotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007734">
        <property type="entry name" value="Heparan_SO4_2-O-STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129:SF17">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="356" mass="41751" checksum="0D62235423A4E5D1" modified="2019-05-08" version="1">MGLLRIMLPPKLQLLAVLTFGVAVLFLENQIQKLEESRGKLERAIARHEVREIEQRHTIDSPRQDATLDEDDDVVIIYNRVPKTASTSFTNIAYDLCAKNKYHVLHINTTKNNPVMSLQDQVRFVKNVTSWKEMKPGFYHGHISYLDFAKFGVKKKPIYINVIRDPIERLVSYYYFLRFGDDYRPGLRRRKQGDKKTFDECVAAGGSDCAPEKLWLQIPFFCGHSSECWNVGSRWALEQAKYNLINEYFLVGVTEELEDFIMLLEATLPRFFRGATELYRTGKKSHLRKTTEKKLPTKETIAKLQLSEIWKMENEFYEFALEQFQFVRAHAVREKDGELYILAQNFFYEKIYPKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GS65</accession>
    <name>A0A452GS65_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM98 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000004836"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005657.1">
        <property type="protein sequence ID" value="ENSGAGP00000004836.1"/>
        <property type="gene ID" value="ENSGAGG00000003971.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018797">
        <property type="entry name" value="FAM98"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31353">
        <property type="entry name" value="FAM98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31353:SF11">
        <property type="entry name" value="PROTEIN FAM98B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10239">
        <property type="entry name" value="DUF2465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="390" mass="43274" checksum="BA87F613174EF78C" modified="2019-05-08" version="1">MRAREQELKMESDVLDALEALGYKGSLLEEEALSKAVENGLSSPDFSELCVWLSSEIKSVCDLEESITSTAGGEDVESFQLEISGFLKEMACPYSTLVSGDIKDRLKKKEDCLKLLLFLSTELQALQILHSKELKSSHLEKNNEIYQEVQTLCDSLGLSKPSSSDIPLLLSNVESKIKDILSKVKNTHVGKPLLTTPLNPDQTERLEKINDALRSEYECRRRMLMKRLDVTVQSFGWSDRAKVKTDDIARIYQPKRYALSPKTTITFAHLLAAREDLSKIIRTSSGSTREKTTCAINKVLMGRVPDRGGRPTEIEPPPPEMPPWQXGGVEVAEEEAVASVVVVEVEVVSKDGETMGAGGVTVAEVAMEIRMVEEEEDTEDTNNNIVNTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTM4</accession>
    <name>A0A452GTM4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GTM4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005067"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005910.1">
        <property type="protein sequence ID" value="ENSGAGP00000005067.1"/>
        <property type="gene ID" value="ENSGAGG00000002028.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057847">
        <property type="entry name" value="ZMIZ1/ZMIZ2_GBD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF7">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25527">
        <property type="entry name" value="GBD-like_ZMIZ1_ZMIZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="6">
        <location>
            <begin position="672"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="202"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="291"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="796"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="392"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="796"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="812"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="878"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="906"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="963"/>
            <end position="990"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="990" mass="107055" checksum="BB75A6A84876FA9B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTV0</accession>
    <name>A0A452GTV0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in protection from oxidative damage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTV0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005468"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006365.1">
        <property type="protein sequence ID" value="ENSGAGP00000005468.1"/>
        <property type="gene ID" value="ENSGAGG00000004437.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.350.10:FF:000002">
        <property type="entry name" value="Oxidation resistance protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF69">
        <property type="entry name" value="OXIDATION RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="7">
        <location>
            <begin position="37"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="8">
        <location>
            <begin position="662"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="250"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="823" mass="92869" checksum="32A4E95D54B926DA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXK1</accession>
    <name>A0A452GXK1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box/LRR-repeat protein 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">F-box and leucine-rich repeat protein 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Directly interacts with SKP1 and CUL1.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXK1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006846"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007943.1">
        <property type="protein sequence ID" value="ENSGAGP00000006846.1"/>
        <property type="gene ID" value="ENSGAGG00000005520.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22121">
        <property type="entry name" value="F-box_FBXL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000005">
        <property type="entry name" value="F-box/LRR-repeat protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000260">
        <property type="entry name" value="F-box/LRR-repeat protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20872">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20872:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="6"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00062469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00070268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00077971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="379" mass="44100" checksum="785F1AC121B35045" modified="2019-05-08" version="1">MPAPRSEFWNYVPEEILVHIFYYLSLRDRYTAFQVCKHWAAAVSTSSVWHFTEISCDSENEEGMLQSLHQFLSQIKHLRIVFDQSKEINRKNVTHILDMLARQNHKLQKLCIVCKGENPYFYSGQDILQSIRNICQRENQIDLQHIDFRRMPFTLDDGLVGLIATSSPNLRSLFINNRTLVCNVKPETIREVLNVCPKLSTLGVYYASLSNDVFAELIKPEREAFTRLDIFCERLDKYIPVISEELWAAVIQKHPQLCVDLEFDHTVPAWKIPRILKPNIPVGTLQLNTFTYMVNQVRFVTSSYSRTLNRLVLHTTPSDDLNSSLIDLARKCVNLTEIHCYCVVSQEVVDTFLVHCPSLKRYTLKITKERHPWKPTVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXV1</accession>
    <name>A0A452GXV1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polycystin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXV1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006984"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008091.1">
        <property type="protein sequence ID" value="ENSGAGP00000006984.1"/>
        <property type="gene ID" value="ENSGAGG00000005626.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015269">
        <property type="term" value="F:calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000055">
        <property type="entry name" value="Polycystic kidney disease 2-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000052">
        <property type="entry name" value="polycystic kidney disease 2-like 1 protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000405">
        <property type="entry name" value="Polycystin 2 like 1, transient receptor potential cation channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000020">
        <property type="entry name" value="polycystin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013122">
        <property type="entry name" value="PKD1_2_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003915">
        <property type="entry name" value="PKD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051223">
        <property type="entry name" value="Polycystin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046791">
        <property type="entry name" value="Polycystin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877">
        <property type="entry name" value="POLYCYSTIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877:SF196">
        <property type="entry name" value="POLYCYSTIN-2-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18109">
        <property type="entry name" value="Fer4_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08016">
        <property type="entry name" value="PKD_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20519">
        <property type="entry name" value="Polycystin_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01433">
        <property type="entry name" value="POLYCYSTIN2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 17" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="17" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="361"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="497"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="559"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="650"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="672"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="663"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="665"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="674"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="230"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR603915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR603915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="801" mass="92794" checksum="4E4F06C8750AEBF8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GY90</accession>
    <name>A0A452GY90_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GY90"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007148"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008272.1">
        <property type="protein sequence ID" value="ENSGAGP00000007148.1"/>
        <property type="gene ID" value="ENSGAGG00000005744.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000003">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CR-type" evidence="8">
        <location>
            <begin position="54"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="7">
        <location>
            <begin position="54"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="333" mass="37604" checksum="9F178E8D9CB68F0D" modified="2019-05-08" version="1">MKEGGMGGRGGFGSPMDIFNMFFGGGVRMPGRPERRGKTVVHPLPVTLEDLYNGTTRKLSLQKNVICSKCNGCGIREGVDSRCPKCHGTGMEFHVHQLGPSMIQQIQTMCSQCQGQGEWVRPLDRCLTCNGRRVVREKKILNVHLDRGMQDGQRITFHEEGDQVPGLEPGDVVIVLDQKEHPVFQRVGDNLVLRKEISLVDALCGFRQVVRTLDNRTLLVSSQRGDVIRPGSVKCVPNEGMPVYRSPAQKGRLIIHFQVRFPEPGWLSPDRLRQLRTFFPPQEEVMATEDMQEVELSDYFSQPGHWWQPHAGEAYHEDDFEEASHPHVQCHTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYF5</accession>
    <name>A0A452GYF5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">tRNA (guanine(10)-N(2))-methyltransferase TRMT11</fullName>
            <ecNumber evidence="12">2.1.1.214</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">tRNA methyltransferase 11 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of the TRMT11-TRM112 methyltransferase complex, that specifically mediates the S-adenosyl-L-methionine-dependent N(2)-methylation of guanosine nucleotide at position 10 (m2G10) in tRNAs. This is one of the major tRNA (guanine-N(2))-methyltransferases.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>guanosine(10) in tRNA + S-adenosyl-L-methionine = N(2)-methylguanosine(10) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43128"/>
            <dbReference type="Rhea" id="RHEA-COMP:10355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74481"/>
            <dbReference type="EC" id="2.1.1.214"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:43129"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the heterodimeric TRMT11-TRM112 methyltransferase complex; this complex forms an active tRNA methyltransferase, where TRMT112 acts as an activator of the catalytic subunit TRMT11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM11 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.214" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452GYF5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007236"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008366.1">
        <property type="protein sequence ID" value="ENSGAGP00000007236.1"/>
        <property type="gene ID" value="ENSGAGG00000005813.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000069">
        <property type="entry name" value="tRNA (Guanine(10)-N2)-methyltransferase homolog isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016691">
        <property type="entry name" value="TRMT11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059073">
        <property type="entry name" value="TRMT11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25904">
        <property type="entry name" value="Tmrp11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017259">
        <property type="entry name" value="tRNA_mtfrase_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51627">
        <property type="entry name" value="SAM_MT_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019039435" description="tRNA (guanine(10)-N(2))-methyltransferase TRMT11" evidence="16">
        <location>
            <begin position="17"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (guanine(10)-N(2))-methyltransferase TRMT11 N-terminal" evidence="18">
        <location>
            <begin position="22"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="17">
        <location>
            <begin position="203"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF25904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="473" mass="54560" checksum="B696B066E1746D17" modified="2019-05-08" version="1">MRGWAERFMASGGAMARGLLPSYLLLLAQENLEFRLPEIKSLLSLCGGQFSSQQEIRINSPFWILNIPSEEMARGLMKRTVCAKSVFELWGQGKSAEELYTSLKNYPMEKMVPYLQSNSTYKIHIHAFNKTLTQDKKIRRIDALEFLPFEGKVNLKNPEHIFWILEDYGMDPNNVPEDPLDLYFGRWIANGQRELIESYSVKRRHFIGNTSMDACLSFIMANHGRVKANDVVFDPFVGTGGLLISSARFGAYVCGTDIDYNTVHGLGKASRKNQKWRGPDENIRANLRQYGLEKYYLDALVSDASKPIWRKEMLFDAIITDPPYGIREATRRTGSQKEILKATERGTENHISISSNYHLSDIFFDLLNSAAEYLVMGGRLVYWLPVYKPEYTEEIIPRHPCLKLISNCEQMLSSHTSRRLITMEKVKEFEDEDQYSHLLDYQSLLYKGHNSFREKYFSGVTKRIAKEEKDNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H087</accession>
    <name>A0A452H087_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">IMP dehydrogenase/GMP reductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H087"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007766"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008955.1">
        <property type="protein sequence ID" value="ENSGAGP00000007766.1"/>
        <property type="gene ID" value="ENSGAGG00000006195.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050139">
        <property type="entry name" value="GMP_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43170">
        <property type="entry name" value="GMP REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43170:SF3">
        <property type="entry name" value="GMP REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="14042" checksum="13B32B97CB5190C8" modified="2019-05-08" version="1">GKKYQFRHVSSYAGVTFRCLRAKLKSHIVDLIRTFTFRNSKQTYTGIPIMVANMDTVGTFEMAKVMTKVSADFHLSLQKQGNHLFGDRMSCSKSITYFIYVHWELGCKGNCCDKIKNRMVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0P0</accession>
    <name>A0A452H0P0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Latent transforming growth factor beta binding protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the LTBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H0P0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008050"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009273.1">
        <property type="protein sequence ID" value="ENSGAGP00000008050.1"/>
        <property type="gene ID" value="ENSGAGG00000006401.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00053">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000004">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000046">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 1 isoform x2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000056">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050751">
        <property type="entry name" value="ECM_structural_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034:SF204">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00683">
        <property type="entry name" value="TB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 10" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="2" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TB" evidence="13">
        <location>
            <begin position="64"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="266"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="307"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="207"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="207"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="39150" checksum="03DE73ABD74D00D4" modified="2019-05-08" version="1">MEQHCQACALSPTCTHSLHTTVCLTCSITPTCTHSSHAHGTVRLTHSVTHTHHTLVSPPDENQAVCWQEVGPDLVCSRPRLDRQVTYTECCCLYGEAWGMDCALCPARDSDDFEALCNVLRPPSYGPARPGLGLPYEYGPEFVPSYGLPYGPELFTSSAARGPQPGLRPDYDPYALGGYTGRRDSLYGPPTYEAGDFEDLAYVDARPEEPPAPYRRPDSPRIYRPRSPPDPEPDPAWHYPPQPASPFPEQLGQASETHTERFEGLRAEECGVLNGCENGQCVRVPHGFTCRCDPGFRLDTAHMACLDIDECAEAEGPPALCLNGRCLNTDGSYRCLCPRGYVLSQPPPSCVPARPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0X2</accession>
    <name>A0A452H0X2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H0X2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008153"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009387.1">
        <property type="protein sequence ID" value="ENSGAGP00000008153.1"/>
        <property type="gene ID" value="ENSGAGG00000006474.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24112">
        <property type="entry name" value="ASKHA_NBD_NTPDase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.150:FF:000002">
        <property type="entry name" value="Ectonucleoside triphosphate diphosphohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000068">
        <property type="entry name" value="Ectonucleoside triphosphate diphosphohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF38">
        <property type="entry name" value="ECTONUCLEOSIDE TRIPHOSPHATE DIPHOSPHOHYDROLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="485"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="222"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="528" mass="59540" checksum="C138C421ECD335D2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1Q0</accession>
    <name>A0A452H1Q0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H1Q0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008552"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009839.1">
        <property type="protein sequence ID" value="ENSGAGP00000008552.1"/>
        <property type="gene ID" value="ENSGAGG00000006783.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019546985" description="GOLD domain-containing protein" evidence="10">
        <location>
            <begin position="27"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="36"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="224" mass="25339" checksum="5AB141DCDFE68C0F" modified="2019-05-08" version="1">MRVPRPGPGLLGPLLLPLLLAGGCGALSFQLPAQTRKCLKEEMHRDVMVTGEYEVSEAGAPGARTDLRVTDSSGHLLYSKEDAKKGKFAFTTDDYEIYEICFESHAQSVGFRVPDQLIILNVKHGVEARNYEDIAKAEKLKPLEVDLRRLEDLSESIVKDFAYMKQREEEMRDTNESTSTRVLYFSIFSMLCLVGLATWQVCYLRRFFKAKKLIEFATTSCLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2D3</accession>
    <name>A0A452H2D3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 10">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H2D3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008787"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010100.1">
        <property type="protein sequence ID" value="ENSGAGP00000008787.1"/>
        <property type="gene ID" value="ENSGAGG00000006950.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009954">
        <property type="term" value="P:proximal/distal pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000018">
        <property type="entry name" value="Homeobox A10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006711">
        <property type="entry name" value="Hox9_activation_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017112">
        <property type="entry name" value="HXA9/HXB9/HXC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45970">
        <property type="entry name" value="AGAP004664-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45970:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-C9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04617">
        <property type="entry name" value="Hox9_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037109">
        <property type="entry name" value="Homeobox_Hox9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="190"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="192"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="142"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="260" mass="29628" checksum="0FF2525A532E106F" modified="2019-05-08" version="1">MSASGPISNYYVDSLISHENEELLASRFPATGSHPAAARPSGLVPDCADFPSCSFAPKPAVFTTSWAPVHSQSSVVYHPYTHQPHIGADTRYMRTWLEPISGAVSFPGFPPSSRHYGLKPDAFPGRRAECGPVDGRSYPDYMYGSPGDLRDRTPQTIPSPESEAVASSKHKEEKNELDPSNPVANWIHARSTRKKRCPYTKYQTLELEKEFLFNMYLTRDRRYEVARVLNLTERQVKIWFQNRRMKMKKMNKEKTDKEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H433</accession>
    <name>A0A452H433_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Interleukin-18</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H433"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009410"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010812.1">
        <property type="protein sequence ID" value="ENSGAGP00000009410.1"/>
        <property type="gene ID" value="ENSGAGG00000007447.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23298">
        <property type="entry name" value="beta-trefoil_IL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000975">
        <property type="entry name" value="IL-1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10078">
        <property type="entry name" value="INTERLEUKIN-1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10078:SF35">
        <property type="entry name" value="INTERLEUKIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00340">
        <property type="entry name" value="IL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="31562" checksum="427A5EABED18CEF4" modified="2019-05-08" version="1">MQLPSSRNRAGELRAGLASSIAAAKQPKSESGALQGMGRTPGAGSRAASSPPPAVSMRSALPASSRAALPSPPFAAHCAPDSAAGGRVKMSCEEIRVFAVKFGQDFDLYFADDEGLECDYWKEVFPLQHQIFRNVSNQVLVVRLGPENNVMSVFESMTDQEMIAGCGVHFNIHYYRDNRLEDGMSVAFSIMVENKTYCMYCTHEGGEKIVRFREEVVPKEILENSSDIIFIQKSISPRDTQAFKFESSLMRGYFLAFQKGEDLSKLILKRCDEDQVDETTHIIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H462</accession>
    <name>A0A452H462_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H462"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009439"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010846.1">
        <property type="protein sequence ID" value="ENSGAGP00000009439.1"/>
        <property type="gene ID" value="ENSGAGG00000007471.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051567">
        <property type="entry name" value="Unconventional_Myosin_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692">
        <property type="entry name" value="MYOSIN VII, XV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692:SF21">
        <property type="entry name" value="MYOSIN XVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="2">
        <location>
            <begin position="64"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="100" mass="11627" checksum="791545C384B582AE" modified="2019-05-08" version="1">MPGEQLRVLRGYSYTDILFVTIPSKHMLEFNLTNEKLILFSPRAPQVKAMIDYFITELKKDSQYVVAVKSYVTDDRSLLSFHKGDIIHLQPLEQPERGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4Y3</accession>
    <name>A0A452H4Y3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribonuclease H</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452H4Y3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009553"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010981.1">
        <property type="protein sequence ID" value="ENSGAGP00000009553.1"/>
        <property type="gene ID" value="ENSGAGG00000007552.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="6">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="5">
        <location>
            <begin position="308"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="640" mass="72541" checksum="D1E99FFF2C83D9CC" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H5G0</accession>
    <name>A0A452H5G0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H5G0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009881"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011351.1">
        <property type="protein sequence ID" value="ENSGAGP00000009881.1"/>
        <property type="gene ID" value="ENSGAGG00000007778.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF207">
        <property type="entry name" value="PYROKININ-1 RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="34"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="287"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="321" mass="35691" checksum="171B449C09764293" modified="2019-05-08" version="1">MERCSFTPVGIWPITPSTAAGVPHGLLFVFGLLANGLSVPTLLQSGRMRASAMRFYLLSLTTADVLMLLTVPVTLYRYFWQYYPWALSDAICKLYFTARQLCCATTSWTIVAFTMERYVAICHPMWAITGLRQLRMAHLLALIWLLALVSSAPLTVVYGQASACILEYTSTRREKSVFLSTVCEMLEPEPYAIYKSIIEARSVLCFLLPLAAIVTFHLLIFRHLSQTCRQREEMGLTQPCSGGFSVQVNQPPGSMPPSERKARHLMGERQPLNGAQRCPRALQHFPEAGQGLQPMSGGEVEGGRPHASRRGLSSPVLRGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H5M5</accession>
    <name>A0A452H5M5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RILP-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Rab-interacting lysosomal-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the RILPL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H5M5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009947"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011420.1">
        <property type="protein sequence ID" value="ENSGAGP00000009947.1"/>
        <property type="gene ID" value="ENSGAGG00000007819.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14445">
        <property type="entry name" value="RILP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1770:FF:000002">
        <property type="entry name" value="RILP-like protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051241">
        <property type="entry name" value="DZIP_RILPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034743">
        <property type="entry name" value="RH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034744">
        <property type="entry name" value="RH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021563">
        <property type="entry name" value="RILP_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21502:SF6">
        <property type="entry name" value="RILP-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21502">
        <property type="entry name" value="ZINC FINGER PROTEIN DZIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09744">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11461">
        <property type="entry name" value="RILP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161256">
        <property type="entry name" value="RILP dimerisation region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51776">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51777">
        <property type="entry name" value="RH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RH1" evidence="16">
        <location>
            <begin position="7"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="RH2" evidence="17">
        <location>
            <begin position="288"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="74"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="423" mass="49378" checksum="98A0F08D6016D4F2" modified="2019-05-08" version="1">MDAGGGVSSSALEKNVAELTVMDVYDIASLVGQEFERVIDQHGCEAIARLMPKVVRVLEILEVLVSRNHINPEMEELRLELDRLRLERMDRIEKEKKHQKELELVEDVWRGEAQDLLTQIAQLQEENKQLMTNLSHKDINFTEEEFQKHEGMSERERQVMKKLKEVVDKQRDEIRARDRELGLKNEDVEALQQQQNRLMKINHDLRHRITVVEAQGKALIEQKVELEAYLQTKEQEMGTLRAELGKLREKLQGEHSENGEEIKVREPNNDDYLSESEKMALDLKDPNRPRFTLQELRDVLHERNELKSRVFLLQEELLYYKSEETEEETRSPQPTPIINSKTSTQPESGIKRLIFTAIMPMVAAGLIPDDPTLQPIRRLVSLVGIFFKCFILCFVLFCFFVGSFLVLVFCFFCMTLMYFNINC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6D6</accession>
    <name>A0A452H6D6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AarF domain containing kinase 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H6D6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010079"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011568.1">
        <property type="protein sequence ID" value="ENSGAGP00000010079.1"/>
        <property type="gene ID" value="ENSGAGG00000007910.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF19">
        <property type="entry name" value="AARF DOMAIN-CONTAINING PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21113" checksum="11FF66310AD5C42A" modified="2019-05-08" version="1">MARRALKLVSLAAASSGFYLYSSKYLDPNDFGAVRVGRAVATTAVITYDYLTSLRSVPYGSQEYAYLKSQVHLRSAERLRDLCCANRGTFIKVGQHLGALDYLLPEEYTRTLKVLHSQAPQSSMQEIEQVIREDLGKESYGVPRDWNAGVLQLRSEIHWQICVWADSTAPGCSLPWVPGSDSPLLSQAPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6E1</accession>
    <name>A0A452H6E1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RNA methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme by protecting tRNA(His) from cleavage by DICER1. Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Was also reported to mediate dimethylation of pre-miR-145; however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end 5'-phospho-ribonucleoside-RNA + 2 S-adenosyl-L-methionine = a 5'-end (5'-bismethylphospho)-ribonucleoside-RNA + 2 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:58640"/>
            <dbReference type="Rhea" id="RHEA-COMP:15179"/>
            <dbReference type="Rhea" id="RHEA-COMP:15182"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:142777"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5'-end 5'-phospho-ribonucleoside-RNA + S-adenosyl-L-methionine = a 5'-end (5'-methylphospho)-ribonucleoside-RNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:58656"/>
            <dbReference type="Rhea" id="RHEA-COMP:15179"/>
            <dbReference type="Rhea" id="RHEA-COMP:15181"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:142776"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A452H6E1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010084"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011573.1">
        <property type="protein sequence ID" value="ENSGAGP00000010084.1"/>
        <property type="gene ID" value="ENSGAGG00000007915.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000632">
        <property type="term" value="P:negative regulation of pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000138">
        <property type="entry name" value="BCDIN3 domain containing RNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039772">
        <property type="entry name" value="Bin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010675">
        <property type="entry name" value="Bin3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024160">
        <property type="entry name" value="BIN3_SAM-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315">
        <property type="entry name" value="BICOID-INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315:SF1">
        <property type="entry name" value="RNA 5'-MONOPHOSPHATE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06859">
        <property type="entry name" value="Bin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51515">
        <property type="entry name" value="BIN3_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Bin3-type SAM" evidence="12">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="251" mass="28402" checksum="FDC064F759DB8B4C" modified="2019-05-08" version="1">MAHLCCHNCFSAYKSQSQQWWVASRAITSVAQVLASELSVALYRHLLGLQENEASPDQPVVGKILNLLCCDIDAGLIERAQRCSPFPGSISYATLDVMDASARELFLSSYLSRFSRSTFDICFCMSVTMWIHLNHGDDGLVEFLSYLSSRCNYLLIEPQPWKCYRAAARRLRKLGRNDFDHFRSLAINGDMAERITQILTEDGGMELVCCFGSTSWDRSLLLFKSNRLTQNQPIPQSLPCRFFSSAYHQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H847</accession>
    <name>A0A452H847_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ornithine decarboxylase antizyme 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ODC antizyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H847"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010897"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012484.1">
        <property type="protein sequence ID" value="ENSGAGP00000010897.1"/>
        <property type="gene ID" value="ENSGAGG00000008468.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008073">
        <property type="term" value="F:ornithine decarboxylase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075523">
        <property type="term" value="P:viral translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.60:FF:000001">
        <property type="entry name" value="Ornithine decarboxylase antizyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002993">
        <property type="entry name" value="ODC_AZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038581">
        <property type="entry name" value="ODC_AZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279:SF8">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02100">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01337">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0688">Ribosomal frameshifting</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="206" mass="23318" checksum="870957C761B65596" modified="2019-05-08" version="1">MVKSSLQRILNSHCFAREKEGNKSTIIIMPAVLSLRAGQSSSRVSFNCCSNLGPGPRWCGRGNSQRDHNLSANLFYSDNRLNVTEELTSNNRTRILNVQSRLTDAKHVNWRAVLNSNNLYIEIPSGALPEGSKDSFAVLLEFAEEQLQVDHVFICFHKNRDDRAALLRTFSFLGFEIVRPGHPLVPKRPDACFMAYTFERDSSEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8W4</accession>
    <name>A0A452H8W4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Anaphase-promoting complex subunit 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cyclosome subunit 11</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Together with the cullin protein ANAPC2, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins. May recruit the E2 ubiquitin-conjugating enzymes to the complex.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5. Interacts with the cullin domain of ANAPC2. Interacts with UBE2D2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RING-box family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H8W4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011016"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012614.1">
        <property type="protein sequence ID" value="ENSGAGP00000011016.1"/>
        <property type="gene ID" value="ENSGAGG00000008534.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16456">
        <property type="entry name" value="RING-H2_APC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000111">
        <property type="entry name" value="Anaphase-promoting complex subunit 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024991">
        <property type="entry name" value="RING-H2_APC11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12861">
        <property type="entry name" value="zf-ANAPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="34"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="84" mass="9803" checksum="EABA7A7DAFE855FA" modified="2019-05-08" version="1">MKVKIKSWNGVASWLWVANDENCGICRMAFNGCCPDCKVPGDDCPLVWGQCSHCFHMHCILKWLNSQQVQQHCPMCRQEWKFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9L0</accession>
    <name>A0A452H9L0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Syntaxin binding protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H9L0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011503"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013172.1">
        <property type="protein sequence ID" value="ENSGAGP00000011503.1"/>
        <property type="gene ID" value="ENSGAGG00000008876.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019428599" description="Syntaxin binding protein 3" evidence="3">
        <location>
            <begin position="25"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="29206" checksum="D8B57BF2F1DDD455" modified="2019-05-08" version="1">MCFFLYVFKLMCVWFFFLTELKLSVFDDCRKEEEWKIIILDDFTTKLLSSCGKMTDLLAEGITAVENVYKNREPVPHMKAIYFITPTKKSIDGLINDFVSKSSSKYKAAYVYFTDFCPDSLFNKMKSSCSRAIRRCKEINISFFPYESQVFTLDVPDAFFCCYSPTVEKANGKDAIMEAMAEQIVTLCATLEENPGVRYKRICMHPVPQVLHECFTSNLATELLLLNLSLGECLLFLGSLHGFGKQQEPEERGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBC4</accession>
    <name>A0A452HBC4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000012051"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013801.1">
        <property type="protein sequence ID" value="ENSGAGP00000012051.1"/>
        <property type="gene ID" value="ENSGAGG00000009276.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000013815.1">
        <property type="protein sequence ID" value="ENSGAGP00000012062.1"/>
        <property type="gene ID" value="ENSGAGG00000009276.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000013828.1">
        <property type="protein sequence ID" value="ENSGAGP00000012075.1"/>
        <property type="gene ID" value="ENSGAGG00000009276.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08697">
        <property type="entry name" value="C2_Dock-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11698">
        <property type="entry name" value="DHR2_DOCK9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13267">
        <property type="entry name" value="PH_DOCK-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000001">
        <property type="entry name" value="dedicator of cytokinesis protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000016">
        <property type="entry name" value="dedicator of cytokinesis protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000015">
        <property type="entry name" value="dedicator of cytokinesis protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.410:FF:000001">
        <property type="entry name" value="dedicator of cytokinesis protein 9 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037809">
        <property type="entry name" value="C2_Dock-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021816">
        <property type="entry name" value="DOCK_C/D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF77">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11878">
        <property type="entry name" value="DOCK_C-D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="170"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="C2 DOCK-type" evidence="6">
        <location>
            <begin position="636"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="7">
        <location>
            <begin position="1639"/>
            <end position="2066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1184"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1255"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1264"/>
            <end position="1277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2104" mass="240620" checksum="49D2DD01D8203A4C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBG6</accession>
    <name>A0A452HBG6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Corticotropin-releasing factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Binds with high affinity to CRF receptors 2-alpha and 2-beta.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HBG6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012097"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013857.1">
        <property type="protein sequence ID" value="ENSGAGP00000012097.1"/>
        <property type="gene ID" value="ENSGAGG00000009309.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051431">
        <property type="term" value="F:corticotropin-releasing hormone receptor 2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031669">
        <property type="term" value="P:cellular response to nutrient levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000187">
        <property type="entry name" value="CRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024270">
        <property type="entry name" value="Urocortin_II/III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17575">
        <property type="entry name" value="UROCORTIN-2 AND 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17575:SF1">
        <property type="entry name" value="UROCORTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00473">
        <property type="entry name" value="CRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019275153" description="Corticotropin-releasing factor domain-containing protein" evidence="9">
        <location>
            <begin position="22"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Corticotropin-releasing factor" evidence="10">
        <location>
            <begin position="110"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="42"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="151" mass="17101" checksum="6BFECA73D5DFC627" modified="2019-05-08" version="1">MSPTRLVLLLLVACAVQTGLSLKLYKAESLFSCINTALSDAEKSRLEENSLVDRRSFDSPPGKEAPSQQEEEEKEKRTFPGDARYKYLSQAQLKRKMYQNRAKPDRRTKFTLSLDVPTNIMNILFDIAKAKNLRAKAAANAHLMAQIGRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCL7</accession>
    <name>A0A452HCL7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Parvalbumin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the parvalbumin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HCL7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012533"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014360.1">
        <property type="protein sequence ID" value="ENSGAGP00000012533.1"/>
        <property type="gene ID" value="ENSGAGG00000009624.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16254">
        <property type="entry name" value="EFh_parvalbumin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000060">
        <property type="entry name" value="Parvalbumin, thymic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008080">
        <property type="entry name" value="Parvalbumin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653">
        <property type="entry name" value="PARVALBUMIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653:SF2">
        <property type="entry name" value="PARVALBUMIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01697">
        <property type="entry name" value="PARVALBUMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="78"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR608080-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="110" mass="12160" checksum="D5719B4505823CD6" modified="2019-05-08" version="1">MAMRDLLSAEEIEAAVGAFSAAESFCHKKFFEMVGLKKKSQEDIKKIFHILDKDQSGFIEENELKFILKGFTPNGRDLSDKETKTLLAAGDKDGDGKIGTDEFITLVAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDR7</accession>
    <name>A0A452HDR7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HDR7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012957"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014851.1">
        <property type="protein sequence ID" value="ENSGAGP00000012957.1"/>
        <property type="gene ID" value="ENSGAGG00000009945.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140416">
        <property type="term" value="F:transcription regulator inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060395">
        <property type="term" value="P:SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000004">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013790">
        <property type="entry name" value="Dwarfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703:SF67">
        <property type="entry name" value="MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703">
        <property type="entry name" value="SMAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MH2" evidence="4">
        <location>
            <begin position="78"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="242" mass="26314" checksum="A4FDB9C1052A2F5D" modified="2019-05-08" version="1">MFPSSQPVLGEGSLYKHPPRPTPEGLPPSAGEGPWVVCSFSGGLVGVSGGCSVRGLRGSLLTSADPSLAWSTTRDGCWCKLAYWEHRTRVGRLYAVHETSVNIFCELPQGSGFSLGQLQAERRSAAVRRARSKIGRGLLLSQERDGVWAYNRSEHPIFASSPTLGPPGARGLPVRKVLPGYSLQVFDYERAGGQASWRRPGDGPCDPNSIRISFAKGWGPCYSRQFITACPCWLEILLTKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEE0</accession>
    <name>A0A452HEE0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Talin N-terminal F0 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HEE0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013190"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015100.1">
        <property type="protein sequence ID" value="ENSGAGP00000013190.1"/>
        <property type="gene ID" value="ENSGAGG00000010103.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030160">
        <property type="term" value="F:synaptic receptor adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000029">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032425">
        <property type="entry name" value="FERM_f0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051569">
        <property type="entry name" value="SHANK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24135">
        <property type="entry name" value="SH3 AND MULTIPLE ANKYRIN REPEAT DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24135:SF17">
        <property type="entry name" value="SH3 AND MULTIPLE ANKYRIN REPEAT DOMAINS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16511">
        <property type="entry name" value="FERM_f0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Talin N-terminal F0" evidence="2">
        <location>
            <begin position="56"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="134" mass="15353" checksum="50331F23F7765C43" modified="2019-05-08" version="1">MPRSPTSSEDEMAQSFSDYSVESESDSSKEETIYDTIRATSEKPSSRMEDNRSNTLVIRIIIPDLQQTKCIRFNPEASVWVAKQRILCTLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVNQEESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEH2</accession>
    <name>A0A452HEH2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IRG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HEH2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013263"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015183.1">
        <property type="protein sequence ID" value="ENSGAGP00000013263.1"/>
        <property type="gene ID" value="ENSGAGG00000010164.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000541">
        <property type="entry name" value="Immunity related GTPase M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051515">
        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341:SF10">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="IRG-type G" evidence="6">
        <location>
            <begin position="33"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="389" mass="43644" checksum="320B6018AFE45602" modified="2019-05-08" version="1">MALDEFRTAIRHGNLAEAISIVQQRPPQWFNSHTLSIAVTGESGSGKSSLINAMLGRRAGDEGAAETGMLNTMMEPMAYPDPFLPGITFWDMPGMGTSYFPLNTYCEQLNLSRFDFFIIVGSQRFRSEHARLVHEIQRMGKRFYFVRSKADMDLDASRRQRPSSYNEERILEQIREDCRGGIAAEGVGHRKVFVVSSWEPNRYDFPLLRQTLQTELLHLKRHAFLLSLTAVASPIINEKKATLKGEIWKTALFSCLLAVIPIPGLAFLYTFFSFRKHLFQYYSSFGVDDRALSALARQVGNPVWELTAVMTSLGTTPMTALKLLADSVGAAVMVVEYSWKRLPVFGAMVSGGVVLVTTYFMLRKCLASVADDTQRVLSKALEAEREKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HF62</accession>
    <name>A0A452HF62_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mediator of RNA polymerase II transcription subunit 22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 22</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Surfeit locus protein 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HF62"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013465"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015410.1">
        <property type="protein sequence ID" value="ENSGAGP00000013465.1"/>
        <property type="gene ID" value="ENSGAGG00000010288.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009332">
        <property type="entry name" value="Med22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12434">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12434:SF6">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06179">
        <property type="entry name" value="Med22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="172"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="203" mass="22942" checksum="3C542B64BF27D8C2" modified="2019-05-08" version="1">MSQQRVLPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATHSEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAINQRNQQLRSLQEECDKKLISLRDEISVDLYELEEEYYSSSYSLCDTSDLPLCEAYWREDPAVLSPEGLSMPLAAATAEQNITAPQTSTPSHPHVNGHGAGPTEHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFD5</accession>
    <name>A0A452HFD5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFD5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013516"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015473.1">
        <property type="protein sequence ID" value="ENSGAGP00000013516.1"/>
        <property type="gene ID" value="ENSGAGG00000010328.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15404">
        <property type="entry name" value="7tmA_GPR63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000080">
        <property type="entry name" value="probable G-protein coupled receptor 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051880">
        <property type="entry name" value="GPC_Orphan_Receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24245">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24245:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 63-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="98"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="419" mass="47702" checksum="B38EBDC83DFDE868" modified="2019-05-08" version="1">MVFSAVLTSAHSRTLNATFIVYENAYMNFTTPQFLLRSGTTQPLNHSSGAMVTTEISTLLVNPTAILPTQDVFKSLSLPLQIILSAAMIFILLVSFLGNFVVCLMVYQKAAMRSAINILLASLAFADMLLAVLNMPFALITIITTRWIFGDIFCRVSAMFFWLFVIEGVAILLIISIDRFLIIVQRQDKLNPYRAKILIAVSWAASFVVAFPLSVGNPNLQIPSRAPQCVFGYTTSPGYQAYVILVVLISFFIPFMIMLYSFMGILNTVRHNAVRIHSHPDSICLSQASKLGLMSLQRPFQMNIDMSFKTRAFTTILILFIVFIVCWAPFTTYSLIATFNSHFYNKHNFFEISTWLLWLCYLKSALNPLIYYWRIKKFHDACLDLMPKYFKFLPQFPGHTRRRIRPSAIYVCGEHRSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGW2</accession>
    <name>A0A452HGW2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGW2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014113"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016160.1">
        <property type="protein sequence ID" value="ENSGAGP00000014113.1"/>
        <property type="gene ID" value="ENSGAGG00000010741.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13220">
        <property type="entry name" value="PH-GRAM_GRAMDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF1">
        <property type="entry name" value="PROTEIN ASTER-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="VASt" evidence="6">
        <location>
            <begin position="234"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="562" mass="64647" checksum="475CC921FFAC460E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHU2</accession>
    <name>A0A452HHU2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.222</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3-O-(alpha-L-fucosyl)-L-seryl-[EGF-like domain protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-alpha-L-fucosyl)-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70511"/>
            <dbReference type="Rhea" id="RHEA-COMP:17919"/>
            <dbReference type="Rhea" id="RHEA-COMP:17920"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:189632"/>
            <dbReference type="ChEBI" id="CHEBI:189633"/>
            <dbReference type="EC" id="2.4.1.222"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>3-O-(alpha-L-fucosyl)-L-threonyl-[EGF-like domain protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-alpha-L-fucosyl)-L-threonyl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70531"/>
            <dbReference type="Rhea" id="RHEA-COMP:17922"/>
            <dbReference type="Rhea" id="RHEA-COMP:17923"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:189631"/>
            <dbReference type="ChEBI" id="CHEBI:189634"/>
            <dbReference type="EC" id="2.4.1.222"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.222" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452HHU2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014420"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016492.1">
        <property type="protein sequence ID" value="ENSGAGP00000014420.1"/>
        <property type="gene ID" value="ENSGAGG00000010960.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033829">
        <property type="term" value="F:O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000003">
        <property type="entry name" value="Beta-1,3-N-acetylglucosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003378">
        <property type="entry name" value="Fringe-like_glycosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10811">
        <property type="entry name" value="FRINGE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02434">
        <property type="entry name" value="Fringe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="21">
        <location>
            <begin position="116"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="384" mass="43250" checksum="D77FB1D40D94D304" modified="2019-05-08" version="1">MRPAWGSQTLGCGGRSSLSLVGFLPWRWKLTVMRRHLLHCLSGVALLLVTMAFLSKRHRGTQEVGRYQRVGDGTSDILEHQGKMFPADVLGDSSKRQELKEPPGPSKVVSSVWRGLELGEVFIAVKTTKRFHQTRLELLLDTWISKAKEQTYVFTDEEDEALQKRMGDHVIFTNCSTEHSHPALSCKMAAEFDAFLASGLSWFCHLDDDNYLNPQALLKLLSSYSPAQDVYIGKPSLNRPIHTSETMLNNQTRSVRFWFATGGAGFCISRRLAIKMAPWASGSNFLSTSELIRLPDDCTVGYIVECKLGGRLLPNMLFHSHLENLQLIPRPQLLQQVTLSYGVFEKKLNTVELAGPFSQHEDPSRFQSLHCLLYPDTSWCPQPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHW1</accession>
    <name>A0A452HHW1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="12">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">PPIases accelerate the folding of proteins during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452HHW1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014484"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016569.1">
        <property type="protein sequence ID" value="ENSGAGP00000014484.1"/>
        <property type="gene ID" value="ENSGAGG00000011010.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000102">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000002">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051989">
        <property type="entry name" value="FKBP-like_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46046:SF2">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46046">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 12" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019360076" description="peptidylprolyl isomerase" evidence="13">
        <location>
            <begin position="31"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="14">
        <location>
            <begin position="65"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="14">
        <location>
            <begin position="177"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="14">
        <location>
            <begin position="289"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="14">
        <location>
            <begin position="400"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="506"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="544"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="583" mass="64710" checksum="CE9F7A0C8A8479D3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIT5</accession>
    <name>A0A452HIT5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the syntrophin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HIT5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014819"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016931.1">
        <property type="protein sequence ID" value="ENSGAGP00000014819.1"/>
        <property type="gene ID" value="ENSGAGG00000011221.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
        <property type="term" value="C:dystrophin-associated glycoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06801">
        <property type="entry name" value="PDZ_syntrophin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01258">
        <property type="entry name" value="PHsplit_syntrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000304">
        <property type="entry name" value="Beta-1-syntrophin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000307">
        <property type="entry name" value="Beta-1-syntrophin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000052">
        <property type="entry name" value="Syntrophin beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041428">
        <property type="entry name" value="PHsplit_syntrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015482">
        <property type="entry name" value="Syntrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055108">
        <property type="entry name" value="Syntrophin_4th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10554:SF11">
        <property type="entry name" value="BETA-1-SYNTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10554">
        <property type="entry name" value="SYNTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18012">
        <property type="entry name" value="PH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23012">
        <property type="entry name" value="Syntrophin_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="13"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="15">
        <location>
            <begin position="97"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="307"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="523" mass="56846" checksum="ADFFAE9076F592E7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HK14</accession>
    <name>A0A452HK14_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HK14"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015262"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017435.1">
        <property type="protein sequence ID" value="ENSGAGP00000015262.1"/>
        <property type="gene ID" value="ENSGAGG00000011513.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008584">
        <property type="term" value="P:male gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000003">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF158">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="8">
        <location>
            <begin position="113"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="7">
        <location>
            <begin position="319"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="266"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="633" mass="70846" checksum="6E4A20B04DECB6C3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNH2</accession>
    <name>A0A452HNH2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HNH2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016513"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018845.1">
        <property type="protein sequence ID" value="ENSGAGP00000016513.1"/>
        <property type="gene ID" value="ENSGAGG00000012353.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001772">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000056">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002026">
        <property type="entry name" value="Zinc finger protein 160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001532">
        <property type="entry name" value="Zinc finger protein 483"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000038">
        <property type="entry name" value="Zinc finger protein 624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000990">
        <property type="entry name" value="zinc finger protein 629 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000933">
        <property type="entry name" value="zinc finger protein 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376:SF252">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="13">
        <location>
            <begin position="33"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="415"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="443"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="471"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="499"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="527"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="555"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="592" mass="67699" checksum="30EAD28DADCF61EA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNX6</accession>
    <name>A0A452HNX6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">F-box only protein 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the SCF(FBXO11) complex consisting of CUL1, RBX1, SKP1 and FBXO11. Interacts with CIITA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HNX6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016688"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019054.1">
        <property type="protein sequence ID" value="ENSGAGP00000016688.1"/>
        <property type="gene ID" value="ENSGAGG00000012476.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22091">
        <property type="entry name" value="F-box_FBXO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19676">
        <property type="entry name" value="UBR-box_UBR6_FBXO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000003">
        <property type="entry name" value="F-box only protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000005">
        <property type="entry name" value="F-box only protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000006">
        <property type="entry name" value="F-box only protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000007">
        <property type="entry name" value="F-box only protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006633">
        <property type="entry name" value="Carb-bd_sugar_hydrolysis-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047505">
        <property type="entry name" value="F-box_FBXO11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047504">
        <property type="entry name" value="FBXO11_UBR-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022441">
        <property type="entry name" value="Para_beta_helix_rpt-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051550">
        <property type="entry name" value="SCF-Subunits/Alg-Epimerases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03804">
        <property type="entry name" value="para_beta_helix"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990">
        <property type="entry name" value="F-BOX ONLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990:SF20">
        <property type="entry name" value="F-BOX ONLY PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00722">
        <property type="entry name" value="CASH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="F-box" evidence="15">
        <location>
            <begin position="111"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="UBR-type" evidence="16">
        <location>
            <begin position="791"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="13">
        <location>
            <begin position="791"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="885" mass="98743" checksum="03CBFA98F4B4FBB5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRJ8</accession>
    <name>A0A452HRJ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PX domain-containing protein kinase-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Modulator of Na,K-ATPase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Binds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HRJ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017675"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020167.1">
        <property type="protein sequence ID" value="ENSGAGP00000017675.1"/>
        <property type="gene ID" value="ENSGAGG00000013136.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000020171.1">
        <property type="protein sequence ID" value="ENSGAGP00000017678.1"/>
        <property type="gene ID" value="ENSGAGG00000013136.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043271">
        <property type="term" value="P:negative regulation of monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006622">
        <property type="term" value="P:protein targeting to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06871">
        <property type="entry name" value="PX_MONaKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000010">
        <property type="entry name" value="PX domain-containing protein kinase-like protein isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000830">
        <property type="entry name" value="PX domain-containing serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037903">
        <property type="entry name" value="MONaKA_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051837">
        <property type="entry name" value="SortingNexin/PXDomain-PKLike"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999:SF40">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999">
        <property type="entry name" value="PX SERINE/THREONINE KINASE PXK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="13">
        <location>
            <begin position="98"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="172"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="14">
        <location>
            <begin position="626"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="523"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="541"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="567"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="598"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="656" mass="73895" checksum="D4943CEFBEA8C229" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRK3</accession>
    <name>A0A452HRK3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ras-related protein Rab-19</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452HRK3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017682"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020175.1">
        <property type="protein sequence ID" value="ENSGAGP00000017682.1"/>
        <property type="gene ID" value="ENSGAGG00000013139.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01864">
        <property type="entry name" value="Rab19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000887">
        <property type="entry name" value="Ras-related protein Rab-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048040">
        <property type="entry name" value="Rab19/43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00057434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="220" mass="24920" checksum="4EF5C97B89E01F93" modified="2019-05-08" version="1">MMPFSSSGSEEAFDYLFKIILIGDSNVGKTCVVHRFKSGQYHEKQQNTIGVDFTVRSLEIDGKKVKIQVWDTAGQERFRTITQSYYRSAHGAILAYDLTRRSTFESIPHWINEIEKYGAANLVLMLIGNKSDVAENRQVLFEDACTLAEKHGLLAVLETSAKEAQNVDEVFTLMAKELIARNTLQLHGGHPPNNIYLDSRQPPLSLPHWNLRMKVPLSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HS01</accession>
    <name>A0A452HS01_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CXXC-type zinc finger protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with DVL1. Interacts with RBPJ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HS01"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017789"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020290.1">
        <property type="protein sequence ID" value="ENSGAGP00000017789.1"/>
        <property type="gene ID" value="ENSGAGG00000013203.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008327">
        <property type="term" value="F:methyl-CpG binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040388">
        <property type="entry name" value="CXXC4/CXXC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002857">
        <property type="entry name" value="Znf_CXXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13419:SF2">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13419">
        <property type="entry name" value="ZINC FINGER-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02008">
        <property type="entry name" value="zf-CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51058">
        <property type="entry name" value="ZF_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CXXC-type" evidence="13">
        <location>
            <begin position="211"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="262"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="288" mass="31584" checksum="F495B954A27FC6AA" modified="2019-05-08" version="1">MSNSGTPQDADNKPNTLDRNNQEDSQPPINSERRNKSGIISEPLNKSLKKSRPLSHYSTFSSSSLVSEHSEKGTSLANGNEAIVDKSHSTSKHKNISSMLSKSDRASEISSEGQISLQQFAQSTDMLKRVVQEHIPLPNDHGTGISDMEAVSAAETMNSPSDFPYLGAFPINPGLFIMTPAGVFLAESALHMAGLAEYPMQNELASAINSGKKKRKRCGMCPPCRRRINCEQCSSCRNRKTGHQICKFRKCEELKKKPSAALEAATAKRRKSPPQDKSSCIVTEPQRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTH7</accession>
    <name>A0A452HTH7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HTH7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018393"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020961.1">
        <property type="protein sequence ID" value="ENSGAGP00000018393.1"/>
        <property type="gene ID" value="ENSGAGG00000013617.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF11">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="4">
        <location>
            <begin position="36"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="306"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="323"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="343"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="41446" checksum="AAD5377995DC0BD3" modified="2019-05-08" version="1">MYRKKFWSQGKTREVTYLQLVNQAQGYARKWTAGAQTKEDLLDLFILEHLYEQCPSDLRLWLMDQKPENPQHAGQLADQFVDSRAGDGREESRRSRPASTQRESHHGTSQRGPMENPPKRGTSSSRSLRPTQGDPRDMGCYRCGQRGHIRAQCPKLRDRPSRPNPQRVDWVKTQSEEGLHSQETGAGNIPPVDAPGSGFLVYRVGAGLPLRKECIVSLEVDGRKVTGYWDTGTEVTLARPEVVASDRMVPDTYLTLMGVGGTPFKVPVARVHLKWGAKEGPKDVGVHRYLPTDVLMGGDLEDWPSNTQSSLVVTRSQSRQRALHPDNGEGTRPEVQDPNPGARCREAVASDPASKREPVPIPVPAAEFQAISC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTK2</accession>
    <name>A0A452HTK2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MPV17 mitochondrial inner membrane protein like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HTK2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018418"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020990.1">
        <property type="protein sequence ID" value="ENSGAGP00000018418.1"/>
        <property type="gene ID" value="ENSGAGG00000013631.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061668">
        <property type="term" value="P:mitochondrial ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF39">
        <property type="entry name" value="MPV17-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019318643" description="MPV17 mitochondrial inner membrane protein like" evidence="7">
        <location>
            <begin position="32"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22106" checksum="6678E145477EAFA1" modified="2019-05-08" version="1">MAGPLLRSGVRRFPWLCNVLLYGSLFSAGDAAQQLLRRRPGQEPDWAQTRHVALVALSFHGNFNYVWLRGLERALPGRAPGAVLAKVLCDQLVGAPVAILAFYTGMSILQGKENIFSDCKKKFWNTYKTGLMYWPFVQLSNFVLVPVYLRTAYTGLCGFLWAIFICFSQQSGDGTAKSAFLWFRREKVNADGEPSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVE6</accession>
    <name>A0A452HVE6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bridge-like lipid transfer protein family member 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000019098"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021766.1">
        <property type="protein sequence ID" value="ENSGAGP00000019098.1"/>
        <property type="gene ID" value="ENSGAGG00000014055.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033616">
        <property type="entry name" value="BLTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056742">
        <property type="entry name" value="BLTP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056741">
        <property type="entry name" value="BLTP1_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31640:SF1">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31640">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN KIAA1109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25040">
        <property type="entry name" value="BLTP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25039">
        <property type="entry name" value="BLTP1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01220">
        <property type="entry name" value="FSA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bridge-like lipid transfer protein family member 1 C-terminal" evidence="2">
        <location>
            <begin position="2791"/>
            <end position="3394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="676"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="814"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1010"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1340"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2029"/>
            <end position="2070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2118"/>
            <end position="2158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2224"/>
            <end position="2255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2300"/>
            <end position="2319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2496"/>
            <end position="2549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2746"/>
            <end position="2801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="779"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="814"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1010"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1031"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1055"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1361"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2144"/>
            <end position="2153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2224"/>
            <end position="2246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2300"/>
            <end position="2314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2505"/>
            <end position="2518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2522"/>
            <end position="2549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2746"/>
            <end position="2762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2764"/>
            <end position="2777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2785"/>
            <end position="2801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="3409" mass="374965" checksum="0ACC08989906611F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXN6</accession>
    <name>A0A452HXN6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HXN6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019951"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022731.1">
        <property type="protein sequence ID" value="ENSGAGP00000019951.1"/>
        <property type="gene ID" value="ENSGAGG00000014693.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="45"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="319" mass="36011" checksum="097A78034750B9A9" modified="2019-05-08" version="1">MAPSVREWNCTAVMEFILLGFGNGPGCQMIPSVLFLVIYTVTMLGNTTLVLIIRVNSRLHTPMYFFFMNLSLLDLYFTSTIAPKAMASFLSGRKAISYNECATQFFLFCFFLATEGFLLAGMAFDRYTAICNPLLYPVTMSKWVCIQMVVGSYICSCVNSMLQTGFTFMLHYCGCKEIEHFFCDGPPLNSLSCNDTYINNLVMFTLYGLIIAITALIVLISYIYIISTVLRICSAEGRHKAFSTCTSHMLVVTLFYGTTAFMYAQPTWLSSLYPRKALSVFYTFYSILTPMLNPIIYSLKNKEMKTAVGKTLGKFKFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYB1</accession>
    <name>A0A452HYB1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Iodothyronine deiodinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>3'-iodothyronamine + iodide + A + H(+) = 3',5'-diiodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83803"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:233339"/>
            <dbReference type="ChEBI" id="CHEBI:233342"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="4">
            <dbReference type="Rhea" id="RHEA:83805"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>3,3',5'-triiodo-L-thyronine sulfate + iodide + A + H(+) = L-thyroxine sulfate + AH2</text>
            <dbReference type="Rhea" id="RHEA:83835"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:176512"/>
            <dbReference type="ChEBI" id="CHEBI:176513"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="2">
            <dbReference type="Rhea" id="RHEA:83837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3,3'-diiodo-L-thyronine sulfate + iodide + A + H(+) = 3,3',5'-triiodo-L-thyronine sulfate + AH2</text>
            <dbReference type="Rhea" id="RHEA:83831"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:176513"/>
            <dbReference type="ChEBI" id="CHEBI:176515"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="5">
            <dbReference type="Rhea" id="RHEA:83833"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3,3'-diiodo-L-thyronine sulfate + iodide + A + H(+) = 3,3',5-triiodo-L-thyronine sulfate + AH2</text>
            <dbReference type="Rhea" id="RHEA:83751"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:176511"/>
            <dbReference type="ChEBI" id="CHEBI:176515"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="3">
            <dbReference type="Rhea" id="RHEA:83753"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3,3'-diiodothyronamine + iodide + A + H(+) = 3,3',5'-triiodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83795"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:233341"/>
            <dbReference type="ChEBI" id="CHEBI:233343"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="6">
            <dbReference type="Rhea" id="RHEA:83797"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-iodo-L-thyronine + iodide + A + H(+) = 3,3'-diiodo-L-thyronine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83783"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:176514"/>
            <dbReference type="ChEBI" id="CHEBI:232627"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:83785"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>3-iodothyronamine + iodide + A + H(+) = 3,3'-diiodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83827"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:231647"/>
            <dbReference type="ChEBI" id="CHEBI:233341"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="7">
            <dbReference type="Rhea" id="RHEA:83829"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the iodothyronine deiodinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYB1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020155"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022969.1">
        <property type="protein sequence ID" value="ENSGAGP00000020155.1"/>
        <property type="gene ID" value="ENSGAGG00000014870.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004800">
        <property type="term" value="F:thyroxine 5'-deiodinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042404">
        <property type="term" value="P:thyroid hormone catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000643">
        <property type="entry name" value="Iodothyronine_deiodinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11781">
        <property type="entry name" value="IODOTHYRONINE DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11781:SF22">
        <property type="entry name" value="TYPE I IODOTHYRONINE DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00837">
        <property type="entry name" value="T4_deiodinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="8" id="KW-0893">Thyroid hormones biosynthesis</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00093186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00093202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00093206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00093210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="114" mass="13096" checksum="C8DC4D1457A4D446" modified="2019-05-08" version="1">SHEPGPGLGSPKTLILLYVSLCVAVGKILIILFPEMMKRYILKRFSYENWGPTFFSLKYLLFVLKVKWKRLEDEAFLGHNAPNTPVADFRGEVHHILDFMQDNRPLVLNFGSCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYN2</accession>
    <name>A0A452HYN2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HYN2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020255"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023078.1">
        <property type="protein sequence ID" value="ENSGAGP00000020255.1"/>
        <property type="gene ID" value="ENSGAGG00000014941.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="84" mass="9146" checksum="76B087F2589D8A4A" modified="2019-05-08" version="1">MEAYHKPDQQALQALKDAANRLRISSIRATTAAGSGTSLLPSSVLPLSQPAGHLASERKRARFCPLVYLHRPLESLDLSSVRSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYS1</accession>
    <name>A0A452HYS1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYS1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020388"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023230.1">
        <property type="protein sequence ID" value="ENSGAGP00000020388.1"/>
        <property type="gene ID" value="ENSGAGG00000015022.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000171">
        <property type="term" value="P:negative regulation of dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000037">
        <property type="entry name" value="B-cell lymphoma/leukemia 11A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000106">
        <property type="entry name" value="B-cell lymphoma/leukemia 11A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057448">
        <property type="entry name" value="BCL-11A_Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051497">
        <property type="entry name" value="Dev/Hematopoietic_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45993">
        <property type="entry name" value="B-CELL LYMPHOMA/LEUKEMIA 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45993:SF5">
        <property type="entry name" value="B-CELL LYMPHOMA_LEUKEMIA 11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25491">
        <property type="entry name" value="CCHC_BCL-11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="289"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="387"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="533"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="635"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="321"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="407"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="422"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="448"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="535"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="575"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="643"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="667"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="704"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="734" mass="80079" checksum="4D88F546710BDAE6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZ74</accession>
    <name>A0A452HZ74_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBF1-interacting co-repressor CIR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HZ74"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020476"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023326.1">
        <property type="protein sequence ID" value="ENSGAGP00000020476.1"/>
        <property type="gene ID" value="ENSGAGG00000015086.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000023327.1">
        <property type="protein sequence ID" value="ENSGAGP00000020477.1"/>
        <property type="gene ID" value="ENSGAGG00000015086.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040014">
        <property type="entry name" value="CIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019339">
        <property type="entry name" value="CIR_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13151">
        <property type="entry name" value="CBF1 INTERACTING COREPRESSOR CIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13151:SF2">
        <property type="entry name" value="COREPRESSOR INTERACTING WITH RBPJ 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10197">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01083">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBF1-interacting co-repressor CIR N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="461" mass="53734" checksum="C0713A5F83A1AD26" modified="2019-05-08" version="1">MGKSFANFMCKKDFHPASKSNIKKVWMAEQKISYDKKKQEELMQQYLKEQESYDNRLLMGDERVKNGLNFMYEAPPGAKKEETKEQEGETEYKFEWQKVAPREKYAKDDMNIRDQPFGIQVRNVRCIKCHKWGHVNTDRECPLFGLSGINASSVSSDGSGPSMHPSELIAEMRNSGFALKQNVLGRNLTANDPSQEFVASEGDDDPEVEFLKSLSTKQKQKLLRKLDRLEKKKKKDRKKKKQQKKKSKRKHKKHKISSSSSSSSETSVSSSDSETDSKAIQKKMYSKKGKKDKFSEANSSDSEGKAKTKKEKLYDEPSSSHGNKDKDREKHRLLKQDSSGENNKWSSYESERKSQSRNQSTDKKETERNERDSRSQSKDERSGRSSCTSHSNYRQRQIRRQPSRSPSEEWHRKNETRSHSTDVYREKKCRESCGDKCNKVDHKERGRNSRSRSRERDRKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZK6</accession>
    <name>A0A452HZK6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZK6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020630"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023503.1">
        <property type="protein sequence ID" value="ENSGAGP00000020630.1"/>
        <property type="gene ID" value="ENSGAGG00000015201.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000035">
        <property type="entry name" value="C-C chemokine receptor type 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF664">
        <property type="entry name" value="C-C CHEMOKINE RECEPTOR TYPE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="56"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="351" mass="39991" checksum="07B532698781F376" modified="2019-05-08" version="1">VQITGFPGPIPVHPWVLHGSCSVLPPYNNLFNYTDLICEKSHTLQFAQTFLPGFFWIVFVILVYWQYRYRKSITERYLLYLPIADLLLVFTLPFWAKAVSDGWIFKNVMCKIVNSMYKINFYSCMLFLTCISFDRHSTIAQAMKAKNSKRKRLLCSKLVCFGVWLIAIVLCIPEIVYSESKQVSDTTTCKMVCPATVTRTIEVTVLALKITKGFLLPLFVMVTCYAFIIHTLLQGKKSPKHKSLKIIIVIITAFLLSQLPYNDILLVKIIDAYTMVIYDCKISDNIDLGFQITQSIAFLHSCLNPFLYVFAGKGFCKALFKILEDASHCKSQIITAGQILQSLLKQTSIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I009</accession>
    <name>A0A452I009_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">THAP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I009"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020779"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023666.1">
        <property type="protein sequence ID" value="ENSGAGP00000020779.1"/>
        <property type="gene ID" value="ENSGAGG00000015295.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47577">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47577:SF1">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="112" mass="12702" checksum="B653B746BBD974D2" modified="2019-05-08" version="1">MPKACSAINCPNRDTRENRARGLSFHSFPKAHELRKRWMLAVKRIEPGSRRLWIPGAGACLCSQHFTQDEFELYGGQKRLKAGVIPSVFSFKVLLFSGSDRCHISTGQFCFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0T4</accession>
    <name>A0A452I0T4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I0T4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021065"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023991.1">
        <property type="protein sequence ID" value="ENSGAGP00000021065.1"/>
        <property type="gene ID" value="ENSGAGG00000015471.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000506">
        <property type="entry name" value="Zinc finger protein 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000340">
        <property type="entry name" value="zinc finger protein 473 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001289">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000188">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF58">
        <property type="entry name" value="ZINC FINGER PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="204"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="276"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="332"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="360"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="234"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="387" mass="44111" checksum="43283D637492D271" modified="2019-05-08" version="1">MMEVEVVWESNNDTCDTGEEHSGDSSHSSERVGRKSSLQLEVNLDYSQPCSPGCSPAGPSGRQELPVELQCEDKETYETYYQKQGETTAGVFVTSNTNFDLQCEDEDLEFCSSEEPQGGLSEDDEKIILVDAFDEEDLEPITGEALPYRCKKCGASFQDLGELQEHNRIHLTEHSYRCPICSKEFFHAANLRMHKLIHSSDRPHKCLECDKGFIRTADVWRHLRNVHKIERSKGVLGNGMARSRGSTLHQNKHGGGDSDQQCSENKKPAGEESTPYICSMCGKGFRKPNLLSRHKVIHRQDKPHKCQECGKSFVERLKLKRHQQIHSGERPFYCEECGRTFTQLVTLQCHQRIHTGEKPYSCAYCSRRFTVSATLRKHERTHKVDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1F8</accession>
    <name>A0A452I1F8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pleckstrin homology and RhoGEF domain containing G3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000021332"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024297.1">
        <property type="protein sequence ID" value="ENSGAGP00000021332.1"/>
        <property type="gene ID" value="ENSGAGG00000015662.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000114">
        <property type="term" value="P:regulation of establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45924">
        <property type="entry name" value="FI17866P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45924:SF4">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="589"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="698" mass="75294" checksum="9348993709A06705" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1G2</accession>
    <name>A0A452I1G2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I1G2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021305"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024264.1">
        <property type="protein sequence ID" value="ENSGAGP00000021305.1"/>
        <property type="gene ID" value="ENSGAGG00000015640.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14990">
        <property type="entry name" value="7tmA_GPR146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047143">
        <property type="entry name" value="GPER1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037487">
        <property type="entry name" value="GPR146"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226:SF3">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 146-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 182 AND ESTROGEN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="44"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="333" mass="38134" checksum="7107C14370EC2C20" modified="2019-05-08" version="1">MWHCEAFNGTKNSENQHLCHDFHLVLFIFSLLYLIICFPVGLCYNALLVLVNLCNKATMTMPDVYFVNIAIAGLIISAMAPMYLLGPANTKWVIWNINNEVYITLLILFNVSSLVIMYSTTLLSLDYYIERALPRTYMSSVYNTKHVCGFIWGGAMLTSFSSLLFYVCNHVSTKIIECSKMQNKEAADAIMVFIGYVVPALAVLYAFVLILRIRHEATPLDQDTGRLDPSVHRLLIATVCTQFTLWTPYYVTLLVNTFINVQGKLTDENYIRILRFMKGLSKLLAFSSSFVMPLLYRYINKNFPHKLRRLLKRIHCGNQGCSHERTVVQQVMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I240</accession>
    <name>A0A452I240_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I240"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021545"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024535.1">
        <property type="protein sequence ID" value="ENSGAGP00000021545.1"/>
        <property type="gene ID" value="ENSGAGG00000015811.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="259" mass="28992" checksum="0BCA00D79DD08746" modified="2019-05-08" version="1">MSPACSETVSCRSSPYPLQMAPQYSMHPLIGPDVPCTPRLLSCSSLPIMVSYPPPTCQLMPPLAHLALCPQEKIKTMFEQLVVVDHPNIVKLHKYWLDVKDSKARVIFITEYVSSGSLKQFLKKTKKNHKAMNARAWKRWCTQILSALSFLHSCDPPIIHGNLTSDTVFIQHNGLIKIGSVWHRVFANAIPDDLRSPIRIERDEQRNLHFFPPEYGHVADGTAVDIFSFGMCALEVGARRPHYGLQELPEGLSPLGLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I358</accession>
    <name>A0A452I358_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="8">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A452I358"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021953"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025015.1">
        <property type="protein sequence ID" value="ENSGAGP00000021953.1"/>
        <property type="gene ID" value="ENSGAGG00000016118.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd10361">
        <property type="entry name" value="SH2_Fps_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000212">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035849">
        <property type="entry name" value="Fes/Fps/Fer_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH2" evidence="9">
        <location>
            <begin position="21"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="121"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="382" mass="42568" checksum="3E7CD80A8215467A" modified="2019-05-08" version="1">KRNRLGLLFPAETGPLGQQDWYHGSIPRQEAQALLTSEGDFLVCASQGRPGAHVLSVLAGGQCRHFIIQCQKARYQFEPGGVGAPTIPSLIHHHLQSRQVLTTKCPFLLRNPVAKVSPLHCCMPGTPGQGNFGEVYSGRLSYDNTPVAVKTCREHLAPETKHKFLMEARIVRRYSHLNVVRIIGVCARKQPVFIVMELVSGGDFLSFLRSEGSRLWVQDLIHFAVQAAAGMAYLESNGCIHRDLAARNCLVGEGNVLKISDFGMSRQEANGIYASQGGMKHIPIKWTAPEALRYGQYSTESDVWSYGVLLWEIFSLGAAPYPGMSNQQVVNQVERGFRMRAPPRCPVEVYDVMLRCWEASPSLRPKFSAIHQEMTQLHWNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3Q8</accession>
    <name>A0A452I3Q8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NF-X1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFX1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3Q8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022151"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025249.1">
        <property type="protein sequence ID" value="ENSGAGP00000022151.1"/>
        <property type="gene ID" value="ENSGAGG00000016090.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034078">
        <property type="entry name" value="NFX1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360:SF1">
        <property type="entry name" value="NF-X1-TYPE ZINC FINGER PROTEIN NFXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR, X-BOX BINDING 1 NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01422">
        <property type="entry name" value="zf-NF-X1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="446"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="7">
        <location>
            <begin position="530"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="734" mass="81346" checksum="B4D63606DA7A81E8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5Q9</accession>
    <name>A0A452I5Q9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-N-acetylglucosaminidase</fullName>
            <ecNumber evidence="7">3.2.1.50</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides.</text>
            <dbReference type="EC" id="3.2.1.50"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 89 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.50" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452I5Q9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022909"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026102.1">
        <property type="protein sequence ID" value="ENSGAGP00000022909.1"/>
        <property type="gene ID" value="ENSGAGG00000016798.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004561">
        <property type="term" value="F:alpha-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000107">
        <property type="entry name" value="Alpha-N-acetylglucosaminidase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.670">
        <property type="entry name" value="N-acetyl-b-d-glucoasminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007781">
        <property type="entry name" value="NAGLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024732">
        <property type="entry name" value="NAGLU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024733">
        <property type="entry name" value="NAGLU_tim-barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12872">
        <property type="entry name" value="ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12872:SF1">
        <property type="entry name" value="ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05089">
        <property type="entry name" value="NAGLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12972">
        <property type="entry name" value="NAGLU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Alpha-N-acetylglucosaminidase tim-barrel" evidence="9">
        <location>
            <begin position="5"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-N-acetylglucosaminidase C-terminal" evidence="10">
        <location>
            <begin position="348"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="620" mass="70954" checksum="2D802E9E2193A93D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I621</accession>
    <name>A0A452I621_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DDRGK domain containing 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I621"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023012"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026228.1">
        <property type="protein sequence ID" value="ENSGAGP00000023012.1"/>
        <property type="gene ID" value="ENSGAGG00000016885.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044389">
        <property type="term" value="F:ubiquitin-like protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903895">
        <property type="term" value="P:negative regulation of IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990592">
        <property type="term" value="P:protein K69-linked ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019153">
        <property type="entry name" value="DDRGK_dom-contain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050899">
        <property type="entry name" value="DDRGK_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48176">
        <property type="entry name" value="DDRGK DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48176:SF1">
        <property type="entry name" value="DDRGK DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09756">
        <property type="entry name" value="DDRGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019533345" description="DDRGK domain containing 1" evidence="2">
        <location>
            <begin position="21"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="17950" checksum="C215D4CE59541E75" modified="2019-05-08" version="1">MDPVLYVLAAALLAALIVFASRFRGQAQAADEEHRRNVAAVVARPQPHTDERGEGMPRRRRNMGSRMLAQRRAQQAEDGMEEEDDGDEAAEFQLSGKIGAKKQRKLEEKQARKAQREAEEAEREERKKLESKREEERRKEEERIRLEEERQVGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I717</accession>
    <name>A0A452I717_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitoferrin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Mitochondrial iron transporter 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Solute carrier family 25 member 37</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells, thereby playing an essential role in heme biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Fe(2+)(in) = Fe(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28486"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I717"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023417"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026671.1">
        <property type="protein sequence ID" value="ENSGAGP00000023417.1"/>
        <property type="gene ID" value="ENSGAGG00000017159.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048250">
        <property type="term" value="P:iron import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000027">
        <property type="entry name" value="mitoferrin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000031">
        <property type="entry name" value="mitoferrin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758:SF4">
        <property type="entry name" value="MITOFERRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758">
        <property type="entry name" value="MITOFERRIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0410">Iron transport</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 19" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="18">
        <location>
            <begin position="31"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="18">
        <location>
            <begin position="129"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="18">
        <location>
            <begin position="220"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="326" mass="35952" checksum="BD616FFA24F81AC0" modified="2019-05-08" version="1">MELSCGRPAAALPGMEAESPGEDYESLPSGASLSTHMTAGAVAGILEHTVMYPVDSVKTRMQSLQPDPKAQYGSVYGALKKIIHTEGFWRPLRGINVTMLGAGPAHAMYFACYEKMKRTLSDIIHHGGNSHLANGIAGSMATLLHDAVMNPAEVVKQRMQMYNSPYKTVLECIQTVHRTEGLGAFYRSYTTQLTMNVPFQAIHFITYEFMQEQINPRREYNPRSHILSGAIAGAVAAAATTPLDVCKTLLNTQENMALNSVNISGHLSGMANAFRTVYQLGGIPGYFRGVQARVIYQMPSTAIAWSVYEFFKYFLTKHKLEKRAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAY9</accession>
    <name>A0A452IAY9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IAY9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024849"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028272.1">
        <property type="protein sequence ID" value="ENSGAGP00000024849.1"/>
        <property type="gene ID" value="ENSGAGG00000018143.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000002">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="13">
        <location>
            <begin position="51"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="14">
        <location>
            <begin position="250"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="451" mass="50566" checksum="323BACF0F418ADE7" modified="2019-05-08" version="1">MVNRECISIHVGQAGVQIGNACWELFCLEHGIQPDGTFKNQPNKVNNDDSFNTFFSETVTEKHVPRAVMVDLEPTVVDEVRAGAYRQLFHPEQLITGKEDAANNYARGHYTIGKESIDLVLDRIRKLTDACSGLQGFLIFHSFGGGTGSGFTSLLMERLSLDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPIISSERAYHEQLSVAEITSSCFEPNNQMVKCDPRHGKYMACCMLYRGDVVPKDVNVAIAAIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAQVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFAEAREDLAALEKDYEEVGTDSFEEENDGEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBK5</accession>
    <name>A0A452IBK5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leucine rich repeat containing 3C</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IBK5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024903"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028347.1">
        <property type="protein sequence ID" value="ENSGAGP00000024903.1"/>
        <property type="gene ID" value="ENSGAGG00000018200.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF171">
        <property type="entry name" value="LEUCINE RICH REPEAT NEURONAL 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019275952" description="Leucine rich repeat containing 3C" evidence="4">
        <location>
            <begin position="23"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="28898" checksum="B0D2DEA6A1A53E35" modified="2019-05-08" version="1">RHTVSPFTMWLLLQSFVLMTFCFHSATTFSKGCYHAEEGGYKTFRCSNAQLTEVPKDIPNDTNKLYLDFNQIAFLPIDAFRNLPVLLELDLSHNAIVRIESGAFRGLSEHLHSLDLSSNKLVSVNTDVFSNLKAKVNLSSNPWLCDCRLQELIRTVDLVAGSSGSIVCDSSAKEEHVGKPFLQVVTDMDFCSVYKKTTDIAMLVTMFGWFAMVISYLVYYVRQNQEDARRHLEYLKSLPSKQRRSEESSTISTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICB1</accession>
    <name>A0A452ICB1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; polyunsaturated fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ICB1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025441"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028949.1">
        <property type="protein sequence ID" value="ENSGAGP00000025441.1"/>
        <property type="gene ID" value="ENSGAGG00000018594.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00658"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03506">
        <property type="entry name" value="Delta6-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353:SF12">
        <property type="entry name" value="ACYL-COA 6-DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="FATTY ACID DESATURASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015921">
        <property type="entry name" value="FA_sphinglp_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="13">
        <location>
            <begin position="18"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="444" mass="52480" checksum="B1E67C688A17A1C1" modified="2019-05-08" version="1">MGKGGEKGEEVGEREAQNRLYSWEEIRRHNLRTDKWLVIERKVYNITKWAARHPGGSRVISHYAGEDATDAFWAFHLNPSFVQKFLKPLLIGELAPGEPSQDRGKNPQLVDDFRALRKTAEDMNLFKADPLFFSLYLAHIIMMEALAWLLVSYYGTSWITTFILSCILATSQAQAGWLQHDFGHLSVFKKSKWNHIVHKFVIGHLKGASANWWNHRHFQHHAKPNIFKKDPDVNMLHIFVLGDSQPVEYGKKKLKYLPYHHQHEYFFLVFPPFLIPVYFQIQIIMTMIRRRFWADLAWAFSYYMRYFIMYIPFYGVLGSLFLLTFVRFLESHWFVWVTQMNHIPMDIDCEKHRDWLSAQLAATCNIEQSFFNDWFTGHLNFQIEHHLFPMMPRHNYWKIAPLVKSLCTKYGVHYEEKSLGKAFRDIVGSLKKSGALWLDAYLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDI8</accession>
    <name>A0A452IDI8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Paralemmin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Paralemmin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="20">Interacts with dopamine receptor DRD3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Apicolateral cell membrane</location>
            <topology evidence="18">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Basolateral cell membrane</location>
            <topology evidence="19">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Filopodium membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the paralemmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDI8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029314.1">
        <property type="protein sequence ID" value="ENSGAGP00000025769.1"/>
        <property type="gene ID" value="ENSGAGG00000018810.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016327">
        <property type="term" value="C:apicolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031527">
        <property type="term" value="C:filopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004965">
        <property type="entry name" value="Paralemmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498:SF6">
        <property type="entry name" value="PARALEMMIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498">
        <property type="entry name" value="PARALEMMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03285">
        <property type="entry name" value="Paralemmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0133">Cell shape</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0488">Methylation</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0636">Prenylation</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="116"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="245"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="357"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00038823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00040790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00041963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="419" mass="46281" checksum="F92D6FCD24E6C95D" modified="2019-05-08" version="1">MWRRLWGDRVHPAICLSRIAEAGAIQQERLQAIAEKRKRQIEIENKRRQLEDDRRQLQHLKSKALRERWLLEGAPSSASKEDEAMKKQMQEDEVKTKELEETIQRLERELEILENGDSAPSTKEKLGKEVTAKEEKAANAQKTPLGTAKEQKICNSPMKSVEGTDMMKAAMYSVEITVEKDRVTGETKVLSSTTLLPKNHCLQGVKVYEDEMKVVHAVHMEDGALENGVQPLSSSEVDELIHKADEANLSEVSQRDSKTKEHEEAKKGGPGSNLPSQKTTPRKEITGVQAKPMDSPRILASGEGAEPSKEQPVTMIFMGYQNVEDEDETKKVLGLGGTIKAELVVIEDADNKPAVAGAADCTGKDHAPPNGSTVEPMAALPQKEDGQVGEMPGTNITDPKGADQDLDMKKQRCKCCTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDU5</accession>
    <name>A0A452IDU5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDU5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026001"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029572.1">
        <property type="protein sequence ID" value="ENSGAGP00000026001.1"/>
        <property type="gene ID" value="ENSGAGG00000018992.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15225">
        <property type="entry name" value="7tmA_OR10A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="53"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="329" mass="37405" checksum="87E80BA47101E88B" modified="2019-05-08" version="1">MDTPCGTHESRNQTKGNLTTVTEFTLLGFSNHRNLQVPLFSIYLFIYIITLMGNILIILITIDTALQTPMYFFLQVLSFLEICYTSVTIPKMLVDFLSDNRSISFVGCAAQMYFLLFLGISECFLLAAMAYDRYVAICNPLRYRLIMNRRVCLSLVVLSWFSGNMVSLVQTVWVFTLLFCGSNQINYFFCDVLPLIKLSCTDITSYEMQLFTATILVNFTPFSLILVSYVVIISTILKMASTDGRHKAFSTCSSHLIVVTLYYGSRGLIYLRPKSIKSLDSNKVVALMYTTITPILNPIVYSLRNSEVKGAVWRLLWDGLKGKIFSQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IG55</accession>
    <name>A0A452IG55_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">RNA helicase aquarius</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Intron-binding protein of 160 kDa</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Involved in pre-mRNA splicing as component of the spliceosome. Intron-binding spliceosomal protein required to link pre-mRNA splicing and snoRNP (small nucleolar ribonucleoprotein) biogenesis. Plays a key role in position-dependent assembly of intron-encoded box C/D small snoRNP, splicing being required for snoRNP assembly. May act by helping the folding of the snoRNA sequence. Binds to intron of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns (40 nucleotides upstream of the branchpoint) during the late stages of splicing. Has ATP-dependent RNA helicase activity and can unwind double-stranded RNA molecules with a 3' overhang (in vitro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Identified in the spliceosome C complex. Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE. Identified in a pentameric intron-binding (IB) complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is incorporated into the spliceosome as a preassembled complex. The IB complex does not contain PRPF19. Within the spliceosome, interacts with SNRPA1, SF3B1, SF3B3, SF3A1 and SF3A2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15 19">Belongs to the CWF11 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026888"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030564.1">
        <property type="protein sequence ID" value="ENSGAGP00000026888.1"/>
        <property type="gene ID" value="ENSGAGG00000019581.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17935">
        <property type="entry name" value="EEXXQc_AQR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000396">
        <property type="entry name" value="RNA helicase aquarius"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048966">
        <property type="entry name" value="Aquarius_b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048967">
        <property type="entry name" value="Aquarius_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032174">
        <property type="entry name" value="Aquarius_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026300">
        <property type="entry name" value="CWF11_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF5">
        <property type="entry name" value="RNA HELICASE AQUARIUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16399">
        <property type="entry name" value="Aquarius_N_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21143">
        <property type="entry name" value="Aquarius_N_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21144">
        <property type="entry name" value="Aquarius_N_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038901">
        <property type="entry name" value="AQR_cwf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 19" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11 19" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RNA helicase aquarius N-terminal" evidence="23">
        <location>
            <begin position="28"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="RNA helicase aquarius beta-barrel" evidence="24">
        <location>
            <begin position="497"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="RNA helicase aquarius insertion" evidence="25">
        <location>
            <begin position="711"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="21">
        <location>
            <begin position="806"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="22">
        <location>
            <begin position="1119"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="754"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1395"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="765"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1427"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1448"/>
            <end position="1460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR038901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF16399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF21143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF21144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1460" mass="168873" checksum="3B55B10CCE915532" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGE0</accession>
    <name>A0A452IGE0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pro-adrenomedullin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">ADM function is mediated by the CALCRL-RAMP2 and CALCRL-RAMP3 receptor complexes with ADM showing the highest potency for the CALCRL-RAMP2 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenomedullin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IGE0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027033"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030715.1">
        <property type="protein sequence ID" value="ENSGAGP00000027033.1"/>
        <property type="gene ID" value="ENSGAGG00000019662.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031700">
        <property type="term" value="F:adrenomedullin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990410">
        <property type="term" value="P:adrenomedullin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010460">
        <property type="term" value="P:positive regulation of heart rate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003073">
        <property type="term" value="P:regulation of systemic arterial blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051665">
        <property type="entry name" value="Adrenomedullin-reg_peptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021116">
        <property type="entry name" value="Calcitonin/adrenomedullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001710">
        <property type="entry name" value="Pro-ADM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23414">
        <property type="entry name" value="ADRENOMEDULLIN, ADM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23414:SF3">
        <property type="entry name" value="PRO-ADRENOMEDULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00214">
        <property type="entry name" value="Calc_CGRP_IAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00801">
        <property type="entry name" value="ADRENOMEDULN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0027">Amidation</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019441012" description="Pro-adrenomedullin" evidence="12">
        <location>
            <begin position="22"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="135"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="185" mass="20558" checksum="A47BFFDD767BD55B" modified="2019-05-08" version="1">MKLVHVALLYLGSATFLGVHAARLDVASDFKRKWTKWALSRAKRDATPPGTFSGQVAAADVQPLIRSQDVKEDPRVSHPSSPEDARIRVKRYRQSMNGFPHFQAMRLGCRFGTCTIQQLAHQIYQLTDKDKDGAAPVNKISPLGYGRRRRSVPAPSRTQSQSWAWPASGAAPLERVWSAQGRAHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IH20</accession>
    <name>A0A452IH20_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IH20"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027283"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030991.1">
        <property type="protein sequence ID" value="ENSGAGP00000027283.1"/>
        <property type="gene ID" value="ENSGAGG00000019837.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006112">
        <property type="term" value="P:energy reserve metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000058">
        <property type="entry name" value="Adhesion G protein-coupled receptor F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057400">
        <property type="entry name" value="ADGRF3/5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051587">
        <property type="entry name" value="Adhesion_GPCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032471">
        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008078">
        <property type="entry name" value="GPCR_2_Ig-hepta-like_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45813:SF4">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45813">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25387">
        <property type="entry name" value="ADGRF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16489">
        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01695">
        <property type="entry name" value="IGHEPTARCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="899"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="938"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="979"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1014"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1056"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1103"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1130"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="189"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="279"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="375"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="11">
        <location>
            <begin position="726"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="12">
        <location>
            <begin position="897"/>
            <end position="1155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1181" mass="130199" checksum="1147EBA887F0C5D3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452II87</accession>
    <name>A0A452II87_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cadherin Y-type LIR-motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452II87"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027546"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031297.1">
        <property type="protein sequence ID" value="ENSGAGP00000027546.1"/>
        <property type="gene ID" value="ENSGAGG00000020021.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000003">
        <property type="entry name" value="Desmoglein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050971">
        <property type="entry name" value="Cadherin-domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009122">
        <property type="entry name" value="Desmosomal_cadherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025">
        <property type="entry name" value="DESMOGLEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025:SF1">
        <property type="entry name" value="DESMOGLEIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01818">
        <property type="entry name" value="DESMOCADHERN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01819">
        <property type="entry name" value="DESMOGLEIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin Y-type LIR-motif" evidence="12">
        <location>
            <begin position="80"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="346"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="347"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="379" mass="40711" checksum="B6E42F540BA6C855" modified="2019-05-08" version="1">MSNGRWEEQRHLLSGAKYGAMAAGGAMAAGGAMAAGGAMTMGAGTTMMMGAGRARTAGPEVGIDEEFLRDYFNEKADTFADEDEAQVAKDCLLLYCQEEAGSSHGSIGCCSFIEDDLDDHFLDDLGDKFKTLADICLGKHAEVEVGQCSSHFLGQQNAFSSEHTYSLGTSFQPPKPVPLVDPLANINIIVTETSSVAGPTVRPTTGVLDPQFLVTERVLAPASGLQEMLEIPDLPNGHNVRVTERLIRSDDTMPGVLKVHDFPDSQYVVVRERERLLVPSSDLKTSLSIPNLSERQNVVITERVVTPTSGLQASYTTPAEVLGMQSIRGQTTISGSGRQERILITDPLSNQTGLGSEDSSTISKSSNIRKYSTVQYTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJZ0</accession>
    <name>A0A452IJZ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">MAP/microtubule affinity-regulating kinase 3</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1. Phosphorylates PKP2 and KSR1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ. Interacts with PKP2 (via N-terminus). Interacts with CDC25C. Interacts with KSR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452IJZ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028252"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032107.1">
        <property type="protein sequence ID" value="ENSGAGP00000028252.1"/>
        <property type="gene ID" value="ENSGAGG00000020101.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14072">
        <property type="entry name" value="STKc_MARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14407">
        <property type="entry name" value="UBA_MARK3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001032">
        <property type="entry name" value="KP78b, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049508">
        <property type="entry name" value="MARK1-4_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF1">
        <property type="entry name" value="MAP_MICROTUBULE AFFINITY-REGULATING KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="56"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="19">
        <location>
            <begin position="326"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="370"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="538"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="547"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="610"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="665" mass="74536" checksum="466D679653553918" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IK86</accession>
    <name>A0A452IK86_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coiled-coil and C2 domain-containing protein 1B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CC2D1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IK86"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028291"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032147.1">
        <property type="protein sequence ID" value="ENSGAGP00000028291.1"/>
        <property type="gene ID" value="ENSGAGG00000020550.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000032148.1">
        <property type="protein sequence ID" value="ENSGAGP00000028292.1"/>
        <property type="gene ID" value="ENSGAGG00000020550.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08690">
        <property type="entry name" value="C2_Freud-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000104">
        <property type="entry name" value="coiled-coil and C2 domain-containing protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037772">
        <property type="entry name" value="C2_Freud"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039725">
        <property type="entry name" value="CC2D1A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006608">
        <property type="entry name" value="CC2D1A/B_DM14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13076">
        <property type="entry name" value="COILED-COIL AND C2 DOMAIN-CONTAINING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13076:SF5">
        <property type="entry name" value="COILED-COIL AND C2 DOMAIN-CONTAINING PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21528">
        <property type="entry name" value="CC2D1A-B_DM14"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00685">
        <property type="entry name" value="DM14"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="713"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="489"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="529"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00068693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="887" mass="98190" checksum="86D505DE7D88D1C5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IL38</accession>
    <name>A0A452IL38_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein RFT1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 9">Intramembrane glycolipid transporter that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. RFT1 could mediate the translocation of the cytosolically oriented intermediate DolPP-GlcNAc2Man5, produced by ALG11, into the ER lumen where dolichol-linked oligosaccharides assembly continues. However, the intramembrane lipid transporter activity could not be confirmed in vitro.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the RFT1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IL38"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028543"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032417.1">
        <property type="protein sequence ID" value="ENSGAGP00000028543.1"/>
        <property type="gene ID" value="ENSGAGG00000020682.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034203">
        <property type="term" value="P:glycolipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007594">
        <property type="entry name" value="RFT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MULTISPAN TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117:SF5">
        <property type="entry name" value="PROTEIN RFT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04506">
        <property type="entry name" value="Rft-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="405"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="471"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="505" mass="57310" checksum="0A4790E96A180C3E" modified="2019-05-08" version="1">MGSRDVLSETTRLASSSALLQILFRVITFGLNAFTLRYVSKEMIGLVNVRLTLLYSTIVFLAREAFRRACLSGSTKRNWTKTINLLWLTVPLGVCWSLFLGWIWLQLLEVPDPNSVPYYKIGVVAFGLSAVVELLGEPFWVLAQAHLFIRLKVIAESLSIISKCILTVILVVLYPQWGLYIFSLAQLLYTSILVLCYVMYFMKFLGSPEATKKSFPVSRMTDLLPNLIEDQIVVDWKEARLTWSFFKQSFLKQMLTEGERYVMTFLNVLNFGDQGVYDIVNNLGSLVARFIFLPIEESFYIFFAKVLERGTDVKLQKQDDIAMAAAVLESLLKLVLLVGLSITVFGYAYSQLALDIYGGSVLSSGSGPSLLRCYSLYVLLLAVNGVTECFTFASMCKEEVDRFNFVMLALSFTFLCVSYFLTQWQGSVGFILANCFNMSIRIAHSIRYIYNYFKESSYRPLKGLLLSPSLIFVYIISGVITVFSEVSVFFRFHYHSCKSLFISSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILB7</accession>
    <name>A0A452ILB7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILB7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028628"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032518.1">
        <property type="protein sequence ID" value="ENSGAGP00000028628.1"/>
        <property type="gene ID" value="ENSGAGG00000020745.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031434">
        <property type="term" value="F:mitogen-activated protein kinase kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000153">
        <property type="entry name" value="Tribbles homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024104">
        <property type="entry name" value="Tribbles/Ser_Thr_kinase_40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961">
        <property type="entry name" value="SER/THR PROTEIN KINASE-TRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961:SF14">
        <property type="entry name" value="TRIBBLES HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="170"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="424"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="449"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="460" mass="50019" checksum="DC5BE9677338B6CD" modified="2019-05-08" version="1">MLQSGVSPASAAPGAGRGGEASGAAVLISSGLHHPPGSGTRSQGAGRCEPAPRTKRPPRRCPSIAPGPGQWRVGASEAPGAGSSGIGPRGSPAPRLSPAGRPPKMSLNVQVIPVVSPLKKKRLDFEDTPDSEPSKPKRPRLGPVSGLTPCLQPLAQSPRAVEQDSRVLQIGPYILLEPREEGHSYRAVHRHTETEYSCKVYPARSYPETMAPYGNLSPHPNVAQVAEVIQGDQNIYVFFQSGRGDMHSHVRRCKRLPEREAAGLFRQMAEAVAHCHDHGVILRDLKLRKFLFVDRERTKLVLENLEDACLLSGPDDSLSDKHGCPAYVSPEILSSKNSYSGKAADVWSLGVVLYTMLVGRYPFQDTEPATLFSKIRRGVFSIPDGLSPKTRCLIQCLLRKTPSERLTAREILLHPWLANGDAASEDSCINPSGEQGMEQVVPDTWSQKDEEEEEEEDLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IM52</accession>
    <name>A0A452IM52_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphatidylinositol-glycan biosynthesis class X protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Stabilizing subunit of the glycosylphosphatidylinositol-mannosyltransferase I complex which catalyzes the transfer of the first mannose, via an alpha-1,4 bond from a dolichol-phosphate-mannose (Dol-P-Man) to the glucosaminyl acyl phosphatidylinositol (GlcN-(acyl)PI) intermediate to generate alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol and participates in the sixth step of the glycosylphosphatidylinositol-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the PIGX family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IM52"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028916"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032847.1">
        <property type="protein sequence ID" value="ENSGAGP00000028916.1"/>
        <property type="gene ID" value="ENSGAGG00000020945.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013233">
        <property type="entry name" value="PIG-X/PBN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040039">
        <property type="entry name" value="PIGX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28650">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28650:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08320">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00780">
        <property type="entry name" value="PIG-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025095843" description="Phosphatidylinositol-glycan biosynthesis class X protein" evidence="10">
        <location>
            <begin position="28"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="256" mass="28710" checksum="8C44A4561B785B50" modified="2019-05-08" version="1">MQWQWMERVFHWRSILIFRVSLLVCTAGNLSAQATCPDITVTQQLLKEGFHRDLLTKVELGAGDEAIGSCTVAIKEHLPTGLYVDPYELASLQQHNLTEALMIPDTVDVESPEYLATDLAIVVYTKPDPQCASCFKAMLPVHCRYHRPTENDGEALTVLKSPEILIHCHKSFPSVECWKYSEVEAPCSVRNRHTCHWNNVKYKHVNKEVILKVPVGLKQHGTLVCAVTLLTTVLCSSLVLAAVCKYGHFSLVHCSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMR5</accession>
    <name>A0A452IMR5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IMR5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029155"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033117.1">
        <property type="protein sequence ID" value="ENSGAGP00000029155.1"/>
        <property type="gene ID" value="ENSGAGG00000021097.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046642">
        <property type="term" value="P:negative regulation of alpha-beta T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002720">
        <property type="term" value="P:positive regulation of cytokine production involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000406">
        <property type="term" value="P:positive regulation of T cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008063">
        <property type="entry name" value="Fas_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46838">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46838:SF1">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00020">
        <property type="entry name" value="TNFR_c6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01680">
        <property type="entry name" value="TNFACTORR6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57586">
        <property type="entry name" value="TNF receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TNFR-Cys" evidence="2">
        <location>
            <begin position="80"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="1">
        <location>
            <begin position="80"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="187" mass="19659" checksum="61BB26408F18ED50" modified="2019-05-08" version="1">TLCPGSGIPQPFPSPSQSPCIRILLIIPPHRDLGQSHPSHSPAPGSCPASCCGHQHCPSPSLLNLPLIVGKACSSTTCMPCVDDTYTEHPNGLTECMRCKVCDAGARLMAKEKCTYTKNTVCGCAPGDFCRHGAGGDCEMCSASTVCLPGSKVKILGTEFNDNVCEVCPDGTFSATNMSHTCQPWTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IN24</accession>
    <name>A0A452IN24_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IN24"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029264"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033238.1">
        <property type="protein sequence ID" value="ENSGAGP00000029264.1"/>
        <property type="gene ID" value="ENSGAGG00000021170.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060079">
        <property type="term" value="P:excitatory postsynaptic potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008065">
        <property type="entry name" value="NPFF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15044">
        <property type="entry name" value="NEUROPEPTIDE FF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15044:SF0">
        <property type="entry name" value="PRO-FMRFAMIDE-RELATED NEUROPEPTIDE FF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15085">
        <property type="entry name" value="NPFF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038092">
        <property type="entry name" value="FMRFamid-rel_pep_precur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01682">
        <property type="entry name" value="FMRFAMIDEPEP"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="101" mass="11534" checksum="6129F961957E4E02" modified="2019-05-08" version="1">MLSHIHPHHPAPVSSTLCPYLEDHALWPLSDERPPGTLLHSLLYALQRPGRSPSFLFQPQRFARETRGSWGGEGRLSQRGWDSMASQFWSMAVPQRFGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452INK4</accession>
    <name>A0A452INK4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Haloacid dehalogenase-like hydrolase domain-containing 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452INK4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029445"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033435.1">
        <property type="protein sequence ID" value="ENSGAGP00000029445.1"/>
        <property type="gene ID" value="ENSGAGG00000021277.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000081">
        <property type="entry name" value="Haloacid dehalogenase like hydrolase domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006353">
        <property type="entry name" value="HAD-SF_hydro_IIA_CECR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01456">
        <property type="entry name" value="CECR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF43">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00069384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="424" mass="47870" checksum="A4AE5AB7E602F184" modified="2019-05-08" version="1">MCAVMEIITYFFLLQPNIPTFGFLFDIDGVVVRGKTPIPAAKKAFQKLVNSQGQFLVPVVFVTNAGNCLRQKKADQLSHILGVPISQDQVVMSHSPLRMFKCYHDKCVLVSGQGPLLDIAKYLGFCQPITIDILRETYPLLDMVDHDRWPKVLHLPAVELPKIEAVVLFGEPVRWETNLQLIIDVLLTGGWPGKPHHQASYPHIPVLACNMDLMWMAEAQSPRFGHGTFMVCLENIYKKITGKELKYEALMGKPSELTYHYAEYLIRMQAAQRHWKKPIQTLYAIGDNLMTDIYGANLYNRYLEEKNSRKGSKGRIQAKVAAGTRSATISQDDEIDHTWQNELAPATATSCRSVLVCTGVYNPHTEVPSDTRESITETVFHGHRDFRFDPALVEPDHIVPDVDAAVDLIYQLENFAPDQDTTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPB1</accession>
    <name>A0A452IPB1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="domain">
        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IPB1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029761"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033793.1">
        <property type="protein sequence ID" value="ENSGAGP00000029761.1"/>
        <property type="gene ID" value="ENSGAGG00000021484.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034704">
        <property type="term" value="C:calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055074">
        <property type="term" value="P:calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032506">
        <property type="term" value="P:cytokinetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000001">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000003">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000004">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008157">
        <property type="entry name" value="ANX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF29">
        <property type="entry name" value="ANNEXIN A11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01810">
        <property type="entry name" value="ANNEXINXI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 6" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="74"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="510" mass="55508" checksum="CE70A38DD401736B" modified="2019-05-08" version="1">MSYPGYPPAGGYPPAAPGSNPWGGSGYPPINPPPIGLENVANYANQFNPDYMSGMASNLSGTFGGANVPQSIYPPAPGGYPPVPPGGFGQPPSGQQASYGTYPPPGGNPPAGMPAYPGYPGSHMPPPGQQPMTYPGQQPMPLPGQQPVPLPGQQPMPSYPSGPAVNPSMPPYSGSVMPTITPGTSGNRGTITDAPGFDPLRDAEVLRKAMKGFGTDEQAIIDCLGSRSNKQRQKIILSFKTAYGKDLIKDLKSELSGNFEKTILAMLKTPVMYDVCEIKEAIKGAGTDEACLIEIMSSRSNEHIREISRTYKTEFKKTLEEAIRSDTSGHFQRLLISLSQGNRDESTNVDMSLVQRDVQELYAAGENRLGTDESKFNAILCTRSRAHLRAVFSEYQRMCNRDIEKSISREMSGDLESGMVAVVKCMKNTPAFFAERLHKAMKGAGTKDRTLIRIMVSRSEVDLLDIRTEYKRMYGKSLYADITVRLLFYYFSPTVDSCMYHKTPLPTLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRJ1</accession>
    <name>A0A452IRJ1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Platelet-derived growth factor subunit A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Platelet-derived growth factor A chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Platelet-derived growth factor alpha polypeptide</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the PDGF/VEGF growth factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IRJ1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030523"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034635.1">
        <property type="protein sequence ID" value="ENSGAGP00000030523.1"/>
        <property type="gene ID" value="ENSGAGG00000021981.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005161">
        <property type="term" value="F:platelet-derived growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048008">
        <property type="term" value="P:platelet-derived growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00135">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000017">
        <property type="entry name" value="Platelet derived growth factor subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023581">
        <property type="entry name" value="PD_growth_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000072">
        <property type="entry name" value="PDGF/VEGF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006782">
        <property type="entry name" value="PDGF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11633">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11633:SF3">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00341">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04692">
        <property type="entry name" value="PDGF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00141">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00249">
        <property type="entry name" value="PDGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50278">
        <property type="entry name" value="PDGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0339">Growth factor</keyword>
    <keyword evidence="9" id="KW-0497">Mitogen</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019467144" description="Platelet-derived growth factor subunit A" evidence="15">
        <location>
            <begin position="21"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Platelet-derived growth factor (PDGF) family profile" evidence="16">
        <location>
            <begin position="83"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="179"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="211" mass="24462" checksum="3E6F705856686AF1" modified="2019-05-08" version="1">MRTWACILLLGFCYLPFAFPEEAEIPQELIERLAHSEIHSIRDLQRLLEIDSVGYDDVSETNLRSHSVHSAKHVQENRPVPIRRKRSIEEAIPAVCKTRTVIYEIPRSQIDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRIHHRSVKVAKVEYVRKKPKLKEVLVRLEEHMECTCTSSNSNSDYREEETGRPRETGKKRKRKRLKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRY2</accession>
    <name>A0A452IRY2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IRY2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030774"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034902.1">
        <property type="protein sequence ID" value="ENSGAGP00000030774.1"/>
        <property type="gene ID" value="ENSGAGG00000022127.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000056">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000506">
        <property type="entry name" value="Zinc finger protein 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000069">
        <property type="entry name" value="Zinc finger protein 572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000213">
        <property type="entry name" value="Zinc finger protein 624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="401"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="496" mass="54984" checksum="4EAEF9C2437E93F2" modified="2019-05-08" version="1">ISGEIGLISPTHILVCSSLFPFFSQISFLSGAGSDLSLDSLCLPSGVGMVSENEEKPQQEDAEQVEPHATLSGSSKGNDSRSCALPEKAKAYESQHRPEENFSSHSDLITCEIINLEGTCYTCHECGKSFSGSSDLLIHQRIHRGGTPYTCSECGKSFSGSSDLLIHQRIHRGGAPYTCSECGKSFNWSSALITHRRIHTGETPYTCSECGKSFNQSSHLITHRRIHTGEKPYGCSECGKCFSRNSHLITHRKIHTGEKPYGCPECGKHFIDSSALISHQRIHTGERPYICSECGKSFSWHSHLITHHRIHTGEKPYACAECGKRFIGSSALLSHQRIHTSERPYTCSECGKSFNQSSALTTHQRSHTRERPYMCSECGKRFSYNSSLTTHQRIHTGEKPYGCSDCGKCFIDSSSSTLITHQRIHTGETPYTCSECGKSFSQSSSLITHRRIHTGEKPYGCSECGKCFNRSSQLITHRRIHTGEKPYGCSDIEIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IT93</accession>
    <name>A0A452IT93_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Microtubule-associated protein RP/EB family member 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAPRE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IT93"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031329"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035535.1">
        <property type="protein sequence ID" value="ENSGAGP00000031329.1"/>
        <property type="gene ID" value="ENSGAGG00000022515.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1430:FF:000001">
        <property type="entry name" value="microtubule-associated protein RP/EB family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000007">
        <property type="entry name" value="Microtubule-associated protein, RP/EB family, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 14" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="15">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="16">
        <location>
            <begin position="184"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="267" mass="30036" checksum="8A21657D6FDCE70C" modified="2019-05-08" version="1">MAVNVYSTSVTSDNLSRHDMLAWINESLQLNLTKIEHLCSGAAYCQFLDMLFPGSVALKKVKFQAKLEHEYIQNFKILQAGFKRMGVDKIIPVDKLVKGKFQDNFEFVQWFKKFFDANYDGKEYDPVAARQGQETAPAPTLVAPILNKPKKALSSGSAAPQRPIVTQRTTAAPKTGTGMVKKAPIGIVEDESAELIQQINVLKLTVEDLEKERDFYFGKLRNIELICQENEGESDPVLQRIVEILYATDEGFVIPDEGAPQEEQEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITA1</accession>
    <name>A0A452ITA1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Paired amphipathic helix protein Sin3a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Histone deacetylase complex subunit Sin3a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Transcriptional corepressor Sin3a</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression. Required for cortical neuron differentiation and callosal axon elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with ARID4B, BRMS1L, HCFC1, HDAC1, HDAC2, MXI1, SAP30L, SAP130, SFPQ and TOPORS. Interacts with OGT (via TPRs 1-6); the interaction mediates transcriptional repression in parallel with histone deacetylase. Interacts with BAZ2A, MXD1, MXD3, MXD4, MBD2, DACH1, NCOR1, NR4A2, REST, RLIM, SAP30, SETDB1, SMYD2, and SUDS3. Interacts with PHF12 in a complex composed of HDAC1, PHF12 and SAP30. Interacts with TET1; the interaction recruits SIN3A to gene promoters. The large PER complex involved in the histone deacetylation is composed of at least HDAC1, PER2, SFPQ and SIN3A. Interacts with KLF11. Interacts with PPHLN1. Found in a complex with YY1, GON4L and HDAC1. Interacts (via PAH2) with FOXK1. Interacts with FOXK2. Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1. Interacts with SINHCAF. Interacts with SPHK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ITA1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031271"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035468.1">
        <property type="protein sequence ID" value="ENSGAGP00000031271.1"/>
        <property type="gene ID" value="ENSGAGG00000022473.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000001">
        <property type="entry name" value="Paired amphipathic helix protein Sin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000002">
        <property type="entry name" value="Paired amphipathic helix protein SIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000004">
        <property type="entry name" value="Paired amphipathic helix protein Sin3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF2">
        <property type="entry name" value="PAIRED AMPHIPATHIC HELIX PROTEIN SIN3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="9 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="21">
        <location>
            <begin position="549"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="83"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="205"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="397"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1135"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="902"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="222"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="254"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="265"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="410"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1137"/>
            <end position="1156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1272" mass="145238" checksum="0DEF1083C30B09E5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITS3</accession>
    <name>A0A452ITS3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Single-pass membrane and coiled-coil domain-containing protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMCO4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ITS3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031443"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035661.1">
        <property type="protein sequence ID" value="ENSGAGP00000031443.1"/>
        <property type="gene ID" value="ENSGAGG00000022586.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027960">
        <property type="entry name" value="DUF4519"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34644">
        <property type="entry name" value="SINGLE-PASS MEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34644:SF2">
        <property type="entry name" value="SINGLE-PASS MEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15012">
        <property type="entry name" value="DUF4519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="38"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="184" mass="20257" checksum="AB4680123AF048BA" modified="2019-05-08" version="1">RVPLERSREEPPAPLGSARPVPSWCCHVLWAEPGLGRGAAAGAPRARGAGRDGGAAGAGRWEVGGGRARTSGGKDNSKVWHRQPFEVARTVTPLFKDLPWPISWQLNFCFVFCFGGGTTKEPKRRMRQLKGKPKKETSRDKKERKQAMQEARQQITTVVLPTMAVVVLLIVVFVYVATRPNTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWD2</accession>
    <name>A0A452IWD2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMGN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IWD2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032345"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036670.1">
        <property type="protein sequence ID" value="ENSGAGP00000032345.1"/>
        <property type="gene ID" value="ENSGAGG00000023151.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000036671.1">
        <property type="protein sequence ID" value="ENSGAGP00000032346.1"/>
        <property type="gene ID" value="ENSGAGG00000023151.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000079">
        <property type="entry name" value="HMGN_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23087:SF12">
        <property type="entry name" value="NON-HISTONE CHROMOSOMAL PROTEIN HMG-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23087">
        <property type="entry name" value="NONHISTONE CHROMOSOMAL PROTEIN HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01101">
        <property type="entry name" value="HMG14_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00925">
        <property type="entry name" value="NONHISHMG17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00527">
        <property type="entry name" value="HMG17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00355">
        <property type="entry name" value="HMG14_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="106" mass="11208" checksum="1A53352D09138CEF" modified="2019-05-08" version="1">MPRRKVSAADGEAQEEPKRRSARLSAKPAPAKVEPKPKKAAAAATAKGKSEDRKAQSKGKKGPKGKQIEGTNQEETKDNLPAENGDFNSAEAPGSDAAREKEAKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZ66</accession>
    <name>A0A452IZ66_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZ66"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033365"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037805.1">
        <property type="protein sequence ID" value="ENSGAGP00000033365.1"/>
        <property type="gene ID" value="ENSGAGG00000023783.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14510">
        <property type="entry name" value="PTP_VSP_TPTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000053">
        <property type="entry name" value="Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase TPTE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1110:FF:000004">
        <property type="entry name" value="Voltage-sensor containing phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045102">
        <property type="entry name" value="PTP_VSP_TPTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF60">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE TPTE2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase tensin-type" evidence="11">
        <location>
            <begin position="118"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="10">
        <location>
            <begin position="203"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="12">
        <location>
            <begin position="301"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="437" mass="50823" checksum="525C8A62A0088260" modified="2019-05-08" version="1">VDLAISDKEKSIRETLEGISLAIALFFLVDVLLRVFVEGFSNYFKSKLNILDAVIVVGTLLINMVYSFSDFSGADKIPRMVIILRALRIIILIRIFRLASQKKQLEKVTRRMVSENKRRYTKDGFDLDLTYVTDRIIAMSFPSSGKQSFYRNPIKEVARFLDTKHKDHYTIYNLCSEKGYDPVYFHYRVERVFIDDHNVPSLEDMLKFTANVREWMKKDEKNIIAIHCKGGKGRTGTMVCTWLIDSDQFESAKESLDYFGERRTDKSTSSKFQGVETPSQSRYVGYYEILKNKYNLKLPPERQLKIKNIKIYSINGVGKGNGSDLKVQIIVKKKIVFHCVCASQENCRLFFDAENDFAVIGLENCPVICGDVKVRFESRSDLPKGYDDCPFFFWFNTSFIENNRLYLPRSELDNPHKSKMWKIYRETFAVELNFVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZ98</accession>
    <name>A0A452IZ98_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GSG1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZ98"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037858.1">
        <property type="protein sequence ID" value="ENSGAGP00000033416.1"/>
        <property type="gene ID" value="ENSGAGG00000023811.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000037859.1">
        <property type="protein sequence ID" value="ENSGAGP00000033417.1"/>
        <property type="gene ID" value="ENSGAGG00000023811.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012478">
        <property type="entry name" value="GSG-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050579">
        <property type="entry name" value="PMP-22/EMP/MP20-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF43">
        <property type="entry name" value="GERM CELL-SPECIFIC GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07803">
        <property type="entry name" value="GSG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="320" mass="36153" checksum="821FF3AA0CE3CEF8" modified="2019-05-08" version="1">MELLKILPWHRAVLAVILNLLALCLSTTALLGSYWCVGTQKVPKPLCGRGKATKCIGVHVSADGGVTNSSSQDVVHYSWETGDDRFAFRYFHTGMWLSCEENMEGPDEKCRSFIELSPPAERGILWLALGSEMVYISLLVISFILLLIEMLYTGNPVCGLKLNAFAAVSSVLSGLLGMVAHMMYSQVFQATVNLGPEDWRPHSWDYGWAFYMAWASFTCCMASAVTTLNTYTKTVLEFKRNHIKGYDGSFRDQSQHHQCFMPQQQHREQMSSYYQQRDKSLHSISEGVDFYSELQQKVLQQEPGLELDEVLGQSMEEDHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZW4</accession>
    <name>A0A452IZW4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Centromere protein O</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-O/MCM21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZW4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033641"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038106.1">
        <property type="protein sequence ID" value="ENSGAGP00000033641.1"/>
        <property type="gene ID" value="ENSGAGG00000023948.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031511">
        <property type="term" value="C:Mis6-Sim4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd23836">
        <property type="entry name" value="DRWD-C_CENP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23835">
        <property type="entry name" value="DRWD-N_CENP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018464">
        <property type="entry name" value="CENP-O"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14582:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14582">
        <property type="entry name" value="INNER KINETOCHORE SUBUNIT MAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09496">
        <property type="entry name" value="CENP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="16"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="296" mass="33795" checksum="613C9B23C8456C10" modified="2019-05-08" version="1">MEEATAYLRDGVLSHLEKLEAHARKLALKQDETQRQQENLVRLRARIQELRLQRDELQTKVNLQQVRLIRQEGPTSSNVEPVQAAETRGQALLKWKVENVKAMLQAFHLTGLSGKLTKQGVCFCISTAYEGTYLDSYYLDLLIQQPVQIRHHSVPIFIPLEQIAKKYLQTNIKCFLAMLSDHLNAYAGRKYQADQLQDHYSAFLEGTLQRNSLHNVLFFNYDVETESKTFPFRAKLVYGDLTRSLPTEATITCKAGDVSDTLVEKTAAHSNLFRHTALHKAFDSFKRAAESLDQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J2L7</accession>
    <name>A0A452J2L7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J2L7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034652"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039228.1">
        <property type="protein sequence ID" value="ENSGAGP00000034652.1"/>
        <property type="gene ID" value="ENSGAGG00000024625.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000066">
        <property type="entry name" value="Paired mesoderm homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043378">
        <property type="entry name" value="PRRX1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46385">
        <property type="entry name" value="PAIRED MESODERM HOMEOBOX PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46385:SF3">
        <property type="entry name" value="PAIRED MESODERM HOMEOBOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="96"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="11">
        <location>
            <begin position="224"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="98"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="247" mass="27050" checksum="BFBF4EB4CCC19BB3" modified="2019-05-08" version="1">MDSAAAFGVDKQFGPSPGRIPEPPDNPQGRKNFSVSHLLDLEEVAAGMNGRKEPGAEARDGAKGLQEPSGGSSGSEAAPQDSESLSPSRGAAKRKKKQRRNRTTFNSSQLQALERVFERTHYPDAFVREELARRVNLSEARVQVWFQNRRAKFRRNERAMLANRSASLLKSYSQDAAIEQPMAPRPTALSPEYLSWTTTSPYSTVPSYSSSSTATPAQGVNMANSIASLRLKAKEFSLNQSHVPTVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J489</accession>
    <name>A0A452J489_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Splicing factor 3B subunit 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B4 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the 17S U2 SnRNP complex, a ribonucleoprotein complex that contains small nuclear RNA (snRNA) U2 and a number of specific proteins. Part of the SF3B subcomplex of the 17S U2 SnRNP complex. SF3B associates with the splicing subcomplex SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). SF3B4 has been found in complex spliceosome 'B' and 'C' as well. Component of the minor (U12-type spliceosome) spliceosome. Found in a complex with PRMT9, SF3B2 and SF3B4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J489"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035250"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039911.1">
        <property type="protein sequence ID" value="ENSGAGP00000035250.1"/>
        <property type="gene ID" value="ENSGAGG00000025055.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12334">
        <property type="entry name" value="RRM1_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12335">
        <property type="entry name" value="RRM2_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000059">
        <property type="entry name" value="splicing factor 3B subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000141">
        <property type="entry name" value="Splicing factor 3b subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034158">
        <property type="entry name" value="SF3B4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034159">
        <property type="entry name" value="SF3B4_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052084">
        <property type="entry name" value="SF3B4_spliceosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030:SF3">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="72"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="159"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="266"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="281"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="291"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="387"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="443"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="477" mass="50045" checksum="28FADB851F414188" modified="2019-05-08" version="1">MQVDFRPQPPWLPACTATSGLTQDGRCLHRPLSAAGAGSGGVKGRKSPAWPRGGGSAVEMAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFADAPPPPSVPTPVVTSLGPGVNPPGIPPPGSFPPPVPPPGAMPPGMPPAMPPPPMPPGAGAPGPPSGGTPSGGHPPHPHPFPPGGMHHPGMPPMQVHHGPPGMGQHHHPGPPGSGGQPPPRPPHGMPHPGPPPMGMPPRGPHFGSPMGHPGPMAHHGMRGPPPLMPPHGYSGPPRPPPYGYQRVPLPPRPAQRPPVPPRGPLRGPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J6Y0</accession>
    <name>A0A452J6Y0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J6Y0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036257"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041044.1">
        <property type="protein sequence ID" value="ENSGAGP00000036257.1"/>
        <property type="gene ID" value="ENSGAGG00000025691.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="14"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="141" mass="15989" checksum="B254FA09BE8EAE16" modified="2019-05-08" version="1">MSIFMIFFSTGVQSQVQLVQSGAEMKKPGESVKISCKTSGLVWVGRIDPADSETKYSESFKGRFTITTDNSISTTYLQLNSLRTEDTAVYYCARHTVRRSLFKAIQLWRKEKIREREFCVKSCGKNSDTTERKRGCVCGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J746</accession>
    <name>A0A452J746_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tripartite motif-containing protein 54</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May bind and stabilize microtubules during myotubes formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452J746"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036316"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041118.1">
        <property type="protein sequence ID" value="ENSGAGP00000036316.1"/>
        <property type="gene ID" value="ENSGAGG00000025737.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19833">
        <property type="entry name" value="Bbox2_MuRF3_C-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000014">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase MID2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017903">
        <property type="entry name" value="COS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033492">
        <property type="entry name" value="Trim54_Bbox2_Zfn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF17">
        <property type="entry name" value="TRIPARTITE MOTIF CONTAINING 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51262">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="15" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="26"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="20">
        <location>
            <begin position="121"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="COS" evidence="21">
        <location>
            <begin position="271"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="328"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="368" mass="41474" checksum="265FB15F936CCBBB" modified="2019-05-08" version="1">MNFAVGFKPMLGDSHSMDSLEKQLICPICLEMFSKPVVILPCQHNLCRKCANDIFQASNPLWQSRGSSSVSSGGRFRCPSCRHEVVLDRHGVYGLQRNLLVENIIDIYKQESTRPLHAKAEQQLMCDEHEDEKINIYCLSCEVPTCSLCKVFGAHKDCEVAPLPSVYKRQKSELSDGVAMLVAGNDRIQAIVTQMEEICKAIEENSRRQKQHLSQRFDSLYSTLEERKKELVQRISREQEERVSHVRSLIRQYGEHLEISAKLVESAIQAMEEPQMASYLQQSKELLQKITDLSKVSMSSRPEPGYEKMDHFSVSVDNLAEMLRTIEFQTDSLGEDDTDGFAGGDGGDAVADEDKPEALEEAEAVGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7J2</accession>
    <name>A0A452J7J2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinetochore protein NDC80</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
            <location evidence="10">Centromere</location>
            <location evidence="10">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the NDC80/HEC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J7J2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036481"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041314.1">
        <property type="protein sequence ID" value="ENSGAGP00000036481.1"/>
        <property type="gene ID" value="ENSGAGG00000025875.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.30:FF:000002">
        <property type="entry name" value="NDC80, kinetochore complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.30">
        <property type="entry name" value="Ncd80 complex, Ncd80 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040967">
        <property type="entry name" value="DUF5595"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005550">
        <property type="entry name" value="Kinetochore_Ndc80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055260">
        <property type="entry name" value="Ndc80_CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057091">
        <property type="entry name" value="NDC80_loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038273">
        <property type="entry name" value="Ndc80_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18077">
        <property type="entry name" value="DUF5595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03801">
        <property type="entry name" value="Ndc80_HEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24487">
        <property type="entry name" value="NDC80_loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 10" id="KW-0137">Centromere</keyword>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="4 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Ndc80 CH" evidence="13">
        <location>
            <begin position="55"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5595" evidence="14">
        <location>
            <begin position="215"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Kinetochore protein NDC80 loop region" evidence="15">
        <location>
            <begin position="378"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="329"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="466"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="607" mass="69925" checksum="909E9AC08D8E72CA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFZ3</accession>
    <name>A0A452GFZ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Transcription factor E4F1</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="29">E4F transcription factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Putative E3 ubiquitin-protein ligase E4F1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">RING-type E3 ubiquitin transferase E4F1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Transcription factor E4F</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">p120E4F</fullName>
        </alternativeName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000498"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000557.1">
        <property type="protein sequence ID" value="ENSGAGP00000000498.1"/>
        <property type="gene ID" value="ENSGAGG00000000413.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010564">
        <property type="term" value="P:regulation of cell cycle process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001557">
        <property type="entry name" value="Transcription factor E4F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000702">
        <property type="entry name" value="Transcription factor E4F1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000715">
        <property type="entry name" value="Transcription factor E4F1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000086">
        <property type="entry name" value="transcription factor E4F1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002233">
        <property type="entry name" value="transcription factor E4F1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="23" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="10" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0498">Mitosis</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="21" id="KW-0804">Transcription</keyword>
    <keyword evidence="19" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="18" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="16" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="17" id="KW-0862">Zinc</keyword>
    <keyword evidence="14 31" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="33">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="33">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="33">
        <location>
            <begin position="250"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="33">
        <location>
            <begin position="438"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="33">
        <location>
            <begin position="466"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="33">
        <location>
            <begin position="494"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="33">
        <location>
            <begin position="522"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="33">
        <location>
            <begin position="550"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="33">
        <location>
            <begin position="578"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="32">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00053485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00067631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00075006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00076827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00082967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00083030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00083844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="676" mass="75048" checksum="B4EAEC1008619D5D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GG78</accession>
    <name>A0A452GG78_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POU transcription factor family. Class-2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GG78"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000550"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000617.1">
        <property type="protein sequence ID" value="ENSGAGP00000000550.1"/>
        <property type="gene ID" value="ENSGAGG00000000399.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000005">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000001">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000972">
        <property type="entry name" value="TF_octamer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF46">
        <property type="entry name" value="POU DOMAIN, CLASS 2, TRANSCRIPTION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00029">
        <property type="entry name" value="OCTAMER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="POU-specific" evidence="12">
        <location>
            <begin position="113"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="213"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="215"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="55"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="273"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="193"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="302"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="352"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="386" mass="41641" checksum="54BD3D087E18BA4E" modified="2019-05-08" version="1">HTAPGPAPHIPPDPLSVPQDIQQLLQLQQLVLVPGHHLQPPAQFLLPQAQQGQQGLLPTPNLFQLPQQSPGGLLPAPPRAGLPAQPVTRPGLPESHLAHPQPPKCLEPPAHPEEPSDLEELEQFARTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAETMSVDSTLPSPNQLSSPSLGFDGLPGRRRKKRTSIETNVRFALEKSFLANQKPTSEEILLIAEQLNMEKEVIRVWFCNRRQKEKRINPCSSTPMLPAQGKPPGYGPHMVRPGPGVSLPLSQASSSLSTTVTTLSSAVGSLTPSRTGMGGGPPTINSATPPPPNSANPSPQSSSHSAIGLSGLNPSTGVGQWWNPAHYQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GH61</accession>
    <name>A0A452GH61_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="6">PPIase</shortName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Identified in the spliceosome C complex. Interacts with SNW1/SKIP. Interacts with CDC40/PRP17; this interaction leads to CDC40 isomerization. Interacts with RBM22.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452GH61"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000921"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001044.1">
        <property type="protein sequence ID" value="ENSGAGP00000000921.1"/>
        <property type="gene ID" value="ENSGAGG00000000758.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01922">
        <property type="entry name" value="cyclophilin_SpCYP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000008">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="7">
        <location>
            <begin position="17"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>cyclosporin A</name>
            <dbReference type="ChEBI" id="CHEBI:4031"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="70"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>cyclosporin A</name>
            <dbReference type="ChEBI" id="CHEBI:4031"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="99"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>cyclosporin A</name>
            <dbReference type="ChEBI" id="CHEBI:4031"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="109"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>cyclosporin A</name>
            <dbReference type="ChEBI" id="CHEBI:4031"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>cyclosporin A</name>
            <dbReference type="ChEBI" id="CHEBI:4031"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>cyclosporin A</name>
            <dbReference type="ChEBI" id="CHEBI:4031"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001467-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="166" mass="18062" checksum="7BCD1215829773DD" modified="2019-05-08" version="1">MAAVPPDSWQPPAVSMETTMGTVVLELYWKHAPKTCKNFAELARRGYYNGTKFHRIIKDFMIQGGDPTGTGRGGASIYGKQFEDELHPELKFTGAGILAMANAGPDTNGSQFFITLAPTQWLDGKHTIFGRVCQGIGVVNRVGMVETNAQDRPADDVKILKAYPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHE4</accession>
    <name>A0A452GHE4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GHE4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001163.1">
        <property type="protein sequence ID" value="ENSGAGP00000001031.1"/>
        <property type="gene ID" value="ENSGAGG00000000839.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF7">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="START" evidence="2">
        <location>
            <begin position="45"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="164" mass="18576" checksum="1573F3270BABAAE6" modified="2019-05-08" version="1">VLKPSSSPPPKKHVPSKTMSTRESVQIPDDRDFNNFRTECNSDVGWSQTYNKSGIVVWIQILEVERSLHKIKCRMECKDIAAETLYDVLHDIEYRKKWDTNVIETFDIGKLTVNSDVGYYASGPGTLKGQHCPLLKGWRWGQGISDDVGGLVVWDPSILRRSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIS5</accession>
    <name>A0A452GIS5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L11</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GIS5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001569"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001788.1">
        <property type="protein sequence ID" value="ENSGAGP00000001569.1"/>
        <property type="gene ID" value="ENSGAGG00000001266.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="7">
        <location>
            <begin position="56"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="166" mass="18551" checksum="D74DAAC87D9AF267" modified="2019-05-08" version="1">VAQDCEKENPMRKLRICKLCLNICVGESGDRLTRAAKVLEQLTGQTSVFSKENERIAVHCTVRGAKAEEILEKGLKVREYELRKNNFSDTGNFGFGIQEHIDLGIKCDPSIGIFGLDFYVVLGRPGFSTADKKCKTGSIGAKHRIGKEKAMRWFQQKYDGIILPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJW3</accession>
    <name>A0A452GJW3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SS18 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SS18 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GJW3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001989"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002264.1">
        <property type="protein sequence ID" value="ENSGAGP00000001989.1"/>
        <property type="gene ID" value="ENSGAGG00000001604.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007726">
        <property type="entry name" value="SS18_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23107:SF2">
        <property type="entry name" value="PROTEIN SSXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23107">
        <property type="entry name" value="SYNOVIAL SARCOMA ASSOCIATED SS18 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05030">
        <property type="entry name" value="SSXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SS18 N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="48"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="162"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="203"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="341" mass="37343" checksum="34B0EFE710DB58CE" modified="2019-05-08" version="1">MLDENNHLIQCIMDYQNKGKTSECSQYQQMLHTNLVYLATIADSNQNMQSLLPAPPTQNMPMGPGGMSQSGPPPPPRSHNMSSDGMVGGGPPAPHMQNQMNGQMPGPNHMPMQGPGPNQLSMTNSSMNMPSSSHGSMGGYNHSVPSSQSMPVQNQMTLSQGQPMGNYGPRPNINMQPNQGPMMHQQPPPSQQYNMPQGGGQHYQGQQPPMGMMGQVNQGNHMMGQRQIPPYRPPQQGNSQYGQQQDAYQGPPQQQGYPPQQQQYTGQQGYPGQQQGYGPSQSGPGPQYPNYPQGQGQQYAAYRPTQPGPPQPPQQRPYGYDQVQLCLVKCTNSCVFILLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKV3</accession>
    <name>A0A452GKV3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UPF3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RENT3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GKV3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002408"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002761.1">
        <property type="protein sequence ID" value="ENSGAGP00000002408.1"/>
        <property type="gene ID" value="ENSGAGG00000001952.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12727">
        <property type="entry name" value="RRM_like_Smg4_UPF3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000067">
        <property type="entry name" value="regulator of nonsense transcripts 3A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039722">
        <property type="entry name" value="Upf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005120">
        <property type="entry name" value="UPF3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112:SF2">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112">
        <property type="entry name" value="UPF3 REGULATOR OF NONSENSE TRANSCRIPTS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03467">
        <property type="entry name" value="Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="UPF3" evidence="8">
        <location>
            <begin position="18"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="176" mass="20301" checksum="A71B554707D2327A" modified="2019-05-08" version="1">AGQGGGEISQGMQIEPLVVIRRLPPSLTKEQLEEQLHPLPAHDYFEFCTADPSLYPHLYSRAYINFRNPDDILLFRDRFDGYVFIDNKGLEYPAVVEFAPFQKISKKKLKKKDAKAGSIEDDPEYRKFLESYCADEEKICANPETLLGEIEAKTRELIGLFSKGKGKLEEKWEEYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKX8</accession>
    <name>A0A452GKX8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 88B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM88 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GKX8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002768.1">
        <property type="protein sequence ID" value="ENSGAGP00000002413.1"/>
        <property type="gene ID" value="ENSGAGG00000001960.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030165">
        <property type="term" value="F:PDZ domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033355">
        <property type="entry name" value="TMEM88"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28628">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 88-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28628:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 88B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20395" checksum="31E6A995F497411F" modified="2019-05-08" version="1">MSDQEIEDFEVDNLSDTSRMIPSLPPYDMDDQLLPLEPRSTCCCWAWTLLIVTTNSLVFLVNLLLVVTIFAIVLLPTIVVVYFGFQCHSRVLHSSAYYCKTILDDNSSSALIILGFVIMSPLIVVAMAVYCSLARRLRLFLCFQPYARAVYKGVKWRWYEEGGLCSCAKEWSTQVKAWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GL11</accession>
    <name>A0A452GL11_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GL11"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002453"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002811.1">
        <property type="protein sequence ID" value="ENSGAGP00000002453.1"/>
        <property type="gene ID" value="ENSGAGG00000001991.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="183" mass="19596" checksum="158D0B774D86887F" modified="2019-05-08" version="1">MVRRSQESFSKNEMAFGVNLESTALHLLTPGQDPVALHPLAPGQDPMALHPLMPSRDPVALHPFMPGQDPVALHSLMPALDPVALHPLRPGQDPVALHWLMPGQDPVALHLLIPGQDSLALHPLMPGWYPVALHLLIPGQDSMALHPLMPGQDPVALHPLAPGLESASGVKCNSITGFYAVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLA0</accession>
    <name>A0A452GLA0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclin family. Cyclin L subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GLA0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002387"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002737.1">
        <property type="protein sequence ID" value="ENSGAGP00000002387.1"/>
        <property type="gene ID" value="ENSGAGG00000001938.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20590">
        <property type="entry name" value="CYCLIN_CCNL2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20593">
        <property type="entry name" value="CYCLIN_CCNL2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000014">
        <property type="entry name" value="cyclin-L1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000016">
        <property type="entry name" value="cyclin-L1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036580">
        <property type="entry name" value="Cyclin_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="11">
        <location>
            <begin position="117"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="12">
        <location>
            <begin position="228"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="11">
        <location>
            <begin position="232"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="350"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="412"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="491"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="526"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="556" mass="62621" checksum="18601E874B0011D2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLP5</accession>
    <name>A0A452GLP5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GLP5"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003098.1">
        <property type="protein sequence ID" value="ENSGAGP00000002703.1"/>
        <property type="gene ID" value="ENSGAGG00000002171.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33504:SF1">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 90, MEMBER A1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33504">
        <property type="entry name" value="NADH DEHYDROGENASE (UBIQUINONE) 1 BETA SUBCOMPLEX, 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="407" mass="46645" checksum="22ECC18A1C651349" modified="2019-05-08" version="1">MTSEPHSARAATEAERQKRPGKGRGWRGSPPPTHVSRSPSNRDAEAGPLSSRVTMLSGAARRAGCLSRPADDELAAKIIQRAWWSHIDRRLFRLLTHTIRAAEHCITYEIMRRVSPLEAELIKDPSMQCKVRFRFAGHEFPPFIVFKIFHNTGGQGSKYISGKRTISPASEAAVDACKLMGHRKYYDQMIWDELQYQNHKIIDEIDVATVKDYMQYISNLDETPAYFGGRDNCWRKLSLENFPRTMIMYDIMDYAQSGTLSNPLKEKLTFLLLRPQNEELRHDQLMTVSRVRSPTSCPPTATSSSRSYQPSSLPRPSVRRSRQARQKVAKMKRVHALEKEREEQKLDVINRSHLTEHQETMTAPTEDLEDHSVFSDEEWEHEAAKLYAWSQALSLEDIGESSLTPNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GM03</accession>
    <name>A0A452GM03_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class II family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GM03"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002675"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003067.1">
        <property type="protein sequence ID" value="ENSGAGP00000002675.1"/>
        <property type="gene ID" value="ENSGAGG00000001858.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042613">
        <property type="term" value="C:MHC class II protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002504">
        <property type="term" value="P:antigen processing and presentation of peptide or polysaccharide antigen via MHC class II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05767">
        <property type="entry name" value="IgC1_MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.320.10">
        <property type="entry name" value="Class II Histocompatibility Antigen, M Beta Chain, Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014745">
        <property type="entry name" value="MHC_II_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001003">
        <property type="entry name" value="MHC_II_a_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF86">
        <property type="entry name" value="HLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DR ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00993">
        <property type="entry name" value="MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00920">
        <property type="entry name" value="MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0491">MHC II</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="83"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="222" mass="25237" checksum="5BE975F3DD1C57C1" modified="2019-05-08" version="1">AAENMLSQVAFYQRTDPFMFEFDQDEIFHVDLERKETVWRLPDFGKFTIFEAQGALGNMAVLKKNMEIMIQRSNRTQAQTVPPEVTVFPEDPVELGEPNVLICFVDKFSPPVLSVTWLKNGQEVTGGVYETDFYPRQDNSFRKFSYLPFLPSQGDFYDCRVEHWGLPEPFTKHWEAQVPTPVPETTETLLCALGLAVGIIAGTILIIKGMKMNAARNPRGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNW7</accession>
    <name>A0A452GNW7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000003365"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003877.1">
        <property type="protein sequence ID" value="ENSGAGP00000003365.1"/>
        <property type="gene ID" value="ENSGAGG00000002659.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031116">
        <property type="term" value="P:positive regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000002">
        <property type="entry name" value="CAP-Gly domain containing linker protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000001">
        <property type="entry name" value="Putative CAP-Gly domain-containing linker protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032108">
        <property type="entry name" value="CLIP1_ZNF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF44">
        <property type="entry name" value="CAP-GLY DOMAIN-CONTAINING LINKER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16641">
        <property type="entry name" value="CLIP1_ZNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="79"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="235"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="134"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="310"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2069"/>
            <end position="2099"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="404"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="546"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1230"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1360"/>
            <end position="1559"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1602"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1851"/>
            <end position="1986"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="2103"/>
            <end position="2130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="2077"/>
            <end position="2093"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2216" mass="249935" checksum="1E5CABCF163DEEAD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQ11</accession>
    <name>A0A452GQ11_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein DPCD</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DPCD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQ11"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003787"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004381.1">
        <property type="protein sequence ID" value="ENSGAGP00000003787.1"/>
        <property type="gene ID" value="ENSGAGG00000003080.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026224">
        <property type="entry name" value="DPCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31921">
        <property type="entry name" value="PROTEIN DPCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31921:SF1">
        <property type="entry name" value="PROTEIN DPCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14913">
        <property type="entry name" value="DPCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02065">
        <property type="entry name" value="PROTEINDPCD"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="168" mass="19356" checksum="6BE4CA59689FB4A1" modified="2019-05-08" version="1">MAEEYDLKTSQLLARKWREKTALGSSGKWQVEVGEPTSHTVGALESELIKESSSNPIFVRRDTQTSFQWRIRNLPYPKEVYSVSVEKEQRCCVVRTTNKKYYKKFPIPDMDRYQLPLDAAALSFTYANATLIITYRKPQEILAVEEELQKELQKIKAANDGDGECKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GR79</accession>
    <name>A0A452GR79_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat and SOCS box protein 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Inhibits IFN-beta production through the IRF3 signaling pathway by targeting TBK1 via 'Lys-48'-linked ubiquitination, leading to its proteasomal degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with TBK1; this interaction promotes TBK1 proteasomal degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GR79"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004365"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005128.1">
        <property type="protein sequence ID" value="ENSGAGP00000004365.1"/>
        <property type="gene ID" value="ENSGAGG00000003626.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03727">
        <property type="entry name" value="SOCS_ASB8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037332">
        <property type="entry name" value="ASB8_SOCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24134:SF9">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24134">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN DDB_G0279043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="113"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="146"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="13">
        <location>
            <begin position="231"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="284" mass="31637" checksum="25C3F572043F237F" modified="2019-05-08" version="1">MWYIMQSIQSKYSLSERLIRTITAIRSFPHDNVEDLISRGADVNCMHGILKPLHCACMVADADCVELLLQKGAEVNALDGYNRTALHYAAEKDETCVEILLEYGANPSALDGNKDTPLHWAAFKNNAECVRTLLENGALVNARDYNDDTPLSWAAMKGNLESVSILLDFGAEVRVVNLKGQTPISRLVALLVRGLGTEREDSCFDLLHRAIGHFDLRKNGNMPREVMRDQQLCEKLTLLCSAPGTLKTLSRYALRHSLGVQFLPDAVKELPLPESLKEYLLLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRQ8</accession>
    <name>A0A452GRQ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GET4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GRQ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004540"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005316.1">
        <property type="protein sequence ID" value="ENSGAGP00000004540.1"/>
        <property type="gene ID" value="ENSGAGG00000003749.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071818">
        <property type="term" value="C:BAT3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045048">
        <property type="term" value="P:protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000060">
        <property type="entry name" value="Golgi to ER traffic protein 4 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007317">
        <property type="entry name" value="GET4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12875">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12875:SF0">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04190">
        <property type="entry name" value="GET4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="312"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="330" mass="36723" checksum="976B05BEB4CEDCC2" modified="2019-05-08" version="1">MAAAMMAAAAEQEASKGGGGRNRGGVQRVEGKLRASVEKGEYYEAHQMYRTLFFRYMSQGKHIEARELMYSGALLFFSHSQQNSAADLSMLVLESLEKSDAKVTDELLENLAKVFSLMDPNSPERVAFVSRALKWSSGGSGKLGHPKLHQLLAITLWKEQNYCESRYHFLHSTDGEGCANMLVEYSSSRGYRSEVDMFVAQAVLQFLCLKNKTSASVVFTTYTQKHPSIEKGPPFVQPLLNFIWFLLLAVDGGKLTVFTVLCEQYQPSLKRDPMYNEYLDRIGQLFFGVPPKQTSSYGGLLGNLLNSLMGTGEDDDAEDGQEDSSPIELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GT56</accession>
    <name>A0A452GT56_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Hydroxyacylglutathione hydrolase, mitochondrial</fullName>
            <ecNumber evidence="7">3.1.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Glyoxalase II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(R)-S-lactoylglutathione + H2O = (R)-lactate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25245"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:57474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:25246"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58896"/>
            <dbReference type="ChEBI" id="CHEBI:71261"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:21865"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.6" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452GT56"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005165"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006022.1">
        <property type="protein sequence ID" value="ENSGAGP00000005165.1"/>
        <property type="gene ID" value="ENSGAGG00000004201.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000019">
        <property type="entry name" value="Hydroxyacylglutathione hydrolase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01374">
        <property type="entry name" value="Glyoxalase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017782">
        <property type="entry name" value="Hydroxyacylglutathione_Hdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03413">
        <property type="entry name" value="GSH_gloB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF80">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005457">
        <property type="entry name" value="Glx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019467131" description="Hydroxyacylglutathione hydrolase, mitochondrial" evidence="18">
        <location>
            <begin position="25"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="19">
        <location>
            <begin position="61"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="310" mass="34385" checksum="7C8D361616737DCD" modified="2019-05-08" version="1">MVCGSRRRLGSVLAFLGAAAGLRAGATRPGAGFLHTNSGLRKSKIVTQTNMKVELLPALTDNYMYLIIDEETKEAAIVDPVQPQKVVEVVRKHGVKLTTVLTTHHHWDHAGGNEKLVKMESGLRVYGGDNRVGALTQKVSHSTSFQVGSLHVKCLYTPCHTSGHICYYVTKPNSSEPPAIFTGDTLFVAGCGKFFEGTPEEMYKALIEILGRLDPETRVYCGHEYTINNLKFARHVEPNNTAIQQKLAWAKAKYDSGEPTIPSTIAEEFTYNPFMRVREKTVQQHAGETDPIRTMGAIRKEKDNFRVPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUU2</accession>
    <name>A0A452GUU2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ORM1-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Plays an essential role in the homeostatic regulation of sphingolipid de novo biosynthesis by modulating the activity of the serine palmitoyltransferase (SPT) in response to ceramide levels. When complexed to SPT, the binding of ceramides to its N-terminus stabilizes a conformation that block SPT substrate entry, hence preventing SPT catalytic activity. Through this mechanism, maintains ceramide levels at sufficient concentrations for the production of complex sphingolipids, but which prevents the accumulation of ceramides to levels that trigger apoptosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Ceramide-sensitive subunit of the serine palmitoyltransferase (SPT) complex, which is also composed of SPTLC1, SPTLC2/3 and SPTSSA/B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ORM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GUU2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005526"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006430.1">
        <property type="protein sequence ID" value="ENSGAGP00000005526.1"/>
        <property type="gene ID" value="ENSGAGG00000004479.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000303">
        <property type="term" value="P:regulation of ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007203">
        <property type="entry name" value="ORMDL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12665">
        <property type="entry name" value="ORMDL PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04061">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018147">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR018147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="153" mass="17615" checksum="EF9870A07E7F1671" modified="2019-05-08" version="1">MNVGTAHSEVNPNTRVMNSRGIWLSYVLGIGLLHVILLSIPFFSVPVVWTLTNIIHNMSMYIFLHTVKGTPFETPDQGKARLLTHWEQMDYGVQFTASRKFLTITPIVLYFLTSFYTKYDRIHFIINTISLMSVLIPKLPQLHGVRIFGINKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWL2</accession>
    <name>A0A452GWL2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Ribosomal RNA-processing protein 8</fullName>
            <ecNumber evidence="15">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Probable methyltransferase required to silence rDNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
            <location evidence="1 15">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the methyltransferase superfamily. RRP8 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A452GWL2"/>
    <dbReference type="SMR" id="A0A452GWL2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006499"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007561.1">
        <property type="protein sequence ID" value="ENSGAGP00000006499.1"/>
        <property type="gene ID" value="ENSGAGG00000005261.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005677">
        <property type="term" value="C:chromatin silencing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046015">
        <property type="term" value="P:regulation of transcription by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2150:FF:000001">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000068">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2150">
        <property type="entry name" value="Ribosomal RNA-processing protein 8, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007823">
        <property type="entry name" value="RRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042036">
        <property type="entry name" value="RRP8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05148">
        <property type="entry name" value="Methyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 15" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="164"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="423" mass="46314" checksum="A433E810EF192165" modified="2019-05-08" version="1">MFDEGGWNKEDGGEALSQAILHPQRCPAQHCPQAPRGSLQKQRRRLLMTLQRLEASSCAKPPQSPGPLSGDSESESDSADQAGRKQPRKQRRGLKPRAASSPHIAPSGSGAPQAGPGPEPEPAGRRRRKDCAAEAPRNKAHTPNKEPPSRGAATGGPGPVLSRKQWRNKQKNKRRQKNKFKAGVGKDVGQSLGRGQDVEQRTSVLPPLELSGALRARMEERLQSARFRYINQQLYTSSSQEAARLFQHDPAAFAIYHRGFTRQVGRWPENPVHRIIQYLRGRPASLVVADFGCGDCKIATSVRNKVHSFDLVALSPRVTVCDMAKVPLADESVDVTVFCLALMGTNLREILEEANRVLRPGGMLKVAEVASRFADIRAFVSAVTQLGFQIVSKVRPTPSPHAAGRMGPQRRGLLWVGWACLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWL6</accession>
    <name>A0A452GWL6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GWL6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006501"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007563.1">
        <property type="protein sequence ID" value="ENSGAGP00000006501.1"/>
        <property type="gene ID" value="ENSGAGG00000005263.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="34"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="134" mass="13862" checksum="383E7EC2B517AB1C" modified="2019-05-08" version="1">MRGDWVRAGGWSQDSWVASHDLVEEKQSLSFLWGDFGGPLVCPKGNSFILAGIVGWGINCTASPPPHPGVYIRVSVYAKWIQEVTGSAGPSAFSPAVLSASTTPLGSLSNGLVCSCLALALVAPLLAGLLLPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWQ3</accession>
    <name>A0A452GWQ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GWQ3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006524"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007592.1">
        <property type="protein sequence ID" value="ENSGAGP00000006524.1"/>
        <property type="gene ID" value="ENSGAGG00000005283.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008112">
        <property type="entry name" value="Relaxin_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF70">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01739">
        <property type="entry name" value="RELAXINR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="37"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="209" mass="23235" checksum="7F3B97D82DA50014" modified="2019-05-08" version="1">MDLTQPNTDGISSFENLLANIILRVFVWVIACVTCFGNLFVICMRSFVVTENSQHIMAIKSLCCADGLMGIYLFFIGAFDLKFSGEYNKHAQVWMASIQCQLVGSLAMLSSEVSVLLLTYMTLEKYLCIVFPFSHYGAGKKRTLSMLVVIWLLGFSLTLVPFWCKESFGDYYGRNGVCFPLQSDETERPGARGYSTGIFLGKESSGHGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H142</accession>
    <name>A0A452H142_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Interleukin-6 receptor subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Interleukin-6 signal transducer</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Membrane glycoprotein 130</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Oncostatin-M receptor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H142"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008175"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009415.1">
        <property type="protein sequence ID" value="ENSGAGP00000008175.1"/>
        <property type="gene ID" value="ENSGAGG00000006495.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070102">
        <property type="term" value="P:interleukin-6-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000281">
        <property type="entry name" value="Cytokine receptor like factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000414">
        <property type="entry name" value="Interleukin-6 receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000524">
        <property type="entry name" value="Interleukin-6 receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000542">
        <property type="entry name" value="Interleukin-6 receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000563">
        <property type="entry name" value="interleukin-6 receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000855">
        <property type="entry name" value="Interleukin-6 receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003529">
        <property type="entry name" value="Hematopoietin_rcpt_Gp130_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010457">
        <property type="entry name" value="IgC2-like_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052672">
        <property type="entry name" value="Type1_Cytokine_Rcpt_Type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423:SF1">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06328">
        <property type="entry name" value="Lep_receptor_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01353">
        <property type="entry name" value="HEMATOPO_REC_L_F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="647"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="152"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="250"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="355"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="545"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="684"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="741"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="806"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="879"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="685"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00083382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="942" mass="104315" checksum="01FC2D0254F91177" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1N7</accession>
    <name>A0A452H1N7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Choline/carnitine acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H1N7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008374"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009643.1">
        <property type="protein sequence ID" value="ENSGAGP00000008374.1"/>
        <property type="gene ID" value="ENSGAGG00000006659.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004095">
        <property type="term" value="F:carnitine O-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF74">
        <property type="entry name" value="CARNITINE O-PALMITOYLTRANSFERASE 1, LIVER ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="100" mass="11442" checksum="1F6AAD3A41BFA44E" modified="2019-05-08" version="1">MVNSNYYAMDFLYVVPTSLQAARAGNVIHATLLYRKKLDGQEIKPILLMESTVPLCSAQWERMFNTSRIPGEETGKRGQPKTRNVRNSKQSAEYFRRVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H320</accession>
    <name>A0A452H320_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aquaporin 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H320"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008872"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010198.1">
        <property type="protein sequence ID" value="ENSGAGP00000008872.1"/>
        <property type="gene ID" value="ENSGAGG00000007017.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015670">
        <property type="term" value="P:carbon dioxide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF113">
        <property type="entry name" value="AQUAPORIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="128"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="17545" checksum="1CA8B911BCA1986B" modified="2019-05-08" version="1">LKKHSDNITSGQAVAVELILTFQLVLCYFASTDRHRNANSPATFIGISVALGHLIGIYFTGCSMNPARSFGPAVIVNKFTVHWIFWVGPMTGAILASLIYNVLLYPHLKSLSQRLAILKGTFEMEAEGMEEDHGRKRPKHRQLVPSHAGAGGPHHKGEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3B2</accession>
    <name>A0A452H3B2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger protein unc-98</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Uncoordinated protein 98</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3B2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008899"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010226.1">
        <property type="protein sequence ID" value="ENSGAGP00000008899.1"/>
        <property type="gene ID" value="ENSGAGG00000007033.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002530">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000566">
        <property type="entry name" value="zinc finger protein 133 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000384">
        <property type="entry name" value="Zinc finger protein 550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF178">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="196"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="103"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="392" mass="45369" checksum="39BE5F061A0CE777" modified="2019-05-08" version="1">MLAPFLYGEVQKAYFDMTTEAATDYPQLKVEILVRSGVTTAIRAQQFYEWKYRDSKAPRSQLFDLVHLAQKWLLIVLDRWVSQNDPPMTSKLDEPCLDSLFIPAGDGIMSENEEENPHQEDFQTEEPSVPLSEKSRRKDSQSPDWEEDCEDEWGTENQERNLPGKKWDESPPRERGFRKLKDIIAQQRPHTGEKPHKCSDCGKSFSRRSNLIHQHQTTHMEERPYQCADCLKSFRSSSALVQHQRSHTGEKPYQCSECRSRFLQSSDLIKHQRIHTGERPYQCPACGKCFSQSSSLTEHQRTHTGERPYHCTECGKRFCQSSTLIQHQRIHTGEKPYRCTECGKSFCRSSNLNQHLTIHMIKKPPGCTDCGRGFSQLTNLIIHQRIHSGETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H516</accession>
    <name>A0A452H516_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the OST3/OST6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H516"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009591"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011023.1">
        <property type="protein sequence ID" value="ENSGAGP00000009591.1"/>
        <property type="gene ID" value="ENSGAGG00000007574.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000011025.1">
        <property type="protein sequence ID" value="ENSGAGP00000009593.1"/>
        <property type="gene ID" value="ENSGAGG00000007574.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015693">
        <property type="term" value="P:magnesium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000009">
        <property type="entry name" value="Tumor suppressor candidate 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021149">
        <property type="entry name" value="OligosaccharylTrfase_OST3/OST6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692:SF1">
        <property type="entry name" value="TUMOR SUPPRESSOR CANDIDATE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04756">
        <property type="entry name" value="OST3_OST6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604491" evidence="13">
        <location>
            <begin position="35"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="340" mass="38386" checksum="93454B3FA023BDAA" modified="2019-05-08" version="1">MALGTRASRWLLHLPAGGCCLLVLLLLCVQLGGGQKKKENLLAEKVEQLMEWSSRRSVIRMNGDKFRRFVKAPPRNYSVIVMFTALQPQRQCSVCRQANEEYQVLANSWRYSSAFSNKLFFTIVDYDEGADVFQQLNMNSAPTFMHFPLKGKPKRADTFDLQRIGFAAEQLAKWIADRTDVHIRVFRPPNYSGTIALALLVSLVGGLLYLRRNNLEFIYNKTGWAMAALCVVFAMTSGQMWNHIRGPPYAHKNPQNGQVSYIHGSSQAQFVAESHIILLLNAAITMGMVLLNEAATSKGDVGKRRIICLVGLGLVVFFFSFLLSIFRSKYHGYPYSFLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H5T4</accession>
    <name>A0A452H5T4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Neuritin 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neuritin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H5T4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011502.1">
        <property type="protein sequence ID" value="ENSGAGP00000010020.1"/>
        <property type="gene ID" value="ENSGAGG00000007824.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990138">
        <property type="term" value="P:neuron projection extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026144">
        <property type="entry name" value="Neuritin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15902:SF5">
        <property type="entry name" value="NEURITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15902">
        <property type="entry name" value="NEURITIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15056">
        <property type="entry name" value="NRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17400" checksum="F9B93DF8D8303C71" modified="2019-05-08" version="1">FPAALPLEARAQRATCWRRQEGPHQSAEGYLVRSLASETKCENIYQGFSDCILKLGENMASYEEDDRDEVQGLHTVCRYWDEFHMCAMTALWECQKEAASIWETLKKESRKIKFQGSLFDLCSPNSSQSFSLTNVSNFVVLSISLMVTWLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8A8</accession>
    <name>A0A452H8A8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">E3 ubiquitin-protein ligase MARCHF5</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Membrane-associated RING finger protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Membrane-associated RING-CH protein V</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">RING-type E3 ubiquitin transferase MARCHF5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452H8A8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011002"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012599.1">
        <property type="protein sequence ID" value="ENSGAGP00000011002.1"/>
        <property type="gene ID" value="ENSGAGG00000008521.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16701">
        <property type="entry name" value="RING_CH-C4HC3_MARCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000262">
        <property type="entry name" value="E3 ubiquitin-protein ligase MARCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46283">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="20">
        <location>
            <begin position="9"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="281" mass="31437" checksum="64CE448D486ACC83" modified="2019-05-08" version="1">MSDQTGLALQQTLDRSCWVCFATDEDDRTAEWVRPCRCRGSTKWVHQTCLQRWVDEKQRGNSTARVACPQCNAEYLIVFPKLGPVVYVLDLADRLISKSCPFAAAGIMVGSIYWTAVTYGAVTVMQVVGHKEGLDVMERADPLFLLIGLPTIPVMLILGKMIRWEDYVLRLWRKYSNKLQILNSIFPGIGCPVPRIPAEANPLADHVSATRILCGALVFPTIATIVGKLMFSSVNSNLQRTILGGIAFVAIKGAFKVYFKQQQYLRQAHRKILNYPEQEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8S4</accession>
    <name>A0A452H8S4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Transcription elongation factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with EAF2. Associates with UBR5 and forms a transcription regulatory complex made of CDK9, Pol II, UBR5 and TCEA1/TFIIS. Part of TBP-based Pol II pre-initiation complex (PIC), in which Pol II core assembles with general transcription factors and other specific initiation factors including GTF2E1, GTF2E2, GTF2F1, GTF2F2, TCEA1, ERCC2, ERCC3, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2A1, GTF2A2, GTF2B and TBP; this large multi-subunit PIC complex mediates DNA unwinding and targets Pol II core to the transcription start site where the first phosphodiester bond forms.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17 18">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the TFS-II family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H8S4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011193"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012818.1">
        <property type="protein sequence ID" value="ENSGAGP00000011193.1"/>
        <property type="gene ID" value="ENSGAGG00000008660.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00183">
        <property type="entry name" value="TFIIS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13749">
        <property type="entry name" value="Zn-ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000001">
        <property type="entry name" value="Probable Transcription elongation factor S-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.30:FF:000001">
        <property type="entry name" value="Transcription elongation factor A (SII), 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.10:FF:000002">
        <property type="entry name" value="Transcription elongation factor A (SII), 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035100">
        <property type="entry name" value="TF_IIS-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006289">
        <property type="entry name" value="TFSII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01385">
        <property type="entry name" value="TFSII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF3">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR A PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006704">
        <property type="entry name" value="TF_IIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11 18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 17 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 18" id="KW-0804">Transcription</keyword>
    <keyword evidence="10 18" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="21">
        <location>
            <begin position="5"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS central" evidence="22">
        <location>
            <begin position="139"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="20">
        <location>
            <begin position="258"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="82"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU368078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="300" mass="33606" checksum="B36306DD14ACB8DC" modified="2019-05-08" version="1">MGGEDEIVRIAKRLDKMVSKKSADGAMDLLKELKSMPITLDLLQSTRIGMSVNALRKQSTDEEVISLAKSLIKSWKKLLDASEDKSEEKKRNSSLSASSSKESGDSRDQSSNKRQDPPKTPTTPKITTFPPVPVTCDAVRNKCREMLTSALQTDSDYIAIGADCEHLSAQIEECIYQDVKNTDMKYKNRVRSRISNLKDSKNPELRKNVLCGAITPEQIAVMTSEEMASNELKEIRKAMTKEAIREHQIARTGGTQTDLFTCGKCKKKNCTYTQVQTRSSDEPMTTFVVCNECGNRWKFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H925</accession>
    <name>A0A452H925_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H925"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011305"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012947.1">
        <property type="protein sequence ID" value="ENSGAGP00000011305.1"/>
        <property type="gene ID" value="ENSGAGG00000008745.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF380">
        <property type="entry name" value="TETRASPANIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="232" mass="25923" checksum="D15D1557C83DDB73" modified="2019-05-08" version="1">MAGVSSCMKYSMFIFNFLFWMCGCIILGVSIWMRVSKAAQEDLNLDSSLFSAVDLMIAVGSIIMVLGFLGCCGAMKESQCMLILFFIGLLLILILQVVAGILGAVYKSQVCKRETDLCDLFSVTILPFHHSISLLLQNQCCGLVKGSEDWGSNFNSPYGNFKICECAEKDQNTDLCIFYSNRYIYKKPCSIVIIDFFKHHMVILMGIAFGLAFIEIVGLGFSMSLYCQIQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H949</accession>
    <name>A0A452H949_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">von Willebrand factor A domain containing 5A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H949"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011330"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012974.1">
        <property type="protein sequence ID" value="ENSGAGP00000011330.1"/>
        <property type="gene ID" value="ENSGAGG00000008756.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737:SF6">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00609">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VIT" evidence="2">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="252"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="703" mass="76144" checksum="5F9795710081D0B3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBK5</accession>
    <name>A0A452HBK5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-actinin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HBK5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012147"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013913.1">
        <property type="protein sequence ID" value="ENSGAGP00000012147.1"/>
        <property type="gene ID" value="ENSGAGG00000009350.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21214">
        <property type="entry name" value="CH_ACTN_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21216">
        <property type="entry name" value="CH_ACTN_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000005">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000156">
        <property type="entry name" value="Actinin alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000005">
        <property type="entry name" value="Actinin alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000002">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000003">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014837">
        <property type="entry name" value="EF-hand_Ca_insen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08726">
        <property type="entry name" value="EFhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01184">
        <property type="entry name" value="efhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="43"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="156"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="758"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="799"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="442"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="904" mass="104274" checksum="F79D581E8C3A023F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDL4</accession>
    <name>A0A452HDL4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA damage-inducible transcript 4 protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DDIT4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HDL4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012900"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014785.1">
        <property type="protein sequence ID" value="ENSGAGP00000012900.1"/>
        <property type="gene ID" value="ENSGAGG00000009901.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071889">
        <property type="term" value="F:14-3-3 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.40:FF:000001">
        <property type="entry name" value="DNA damage-inducible transcript 4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.40">
        <property type="entry name" value="RTP801-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012918">
        <property type="entry name" value="RTP801-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038281">
        <property type="entry name" value="RTP801-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478:SF7">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE TRANSCRIPT 4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478">
        <property type="entry name" value="DNA-DAMAGE-INDUCIBLE TRANSCRIPT 4 PROTEIN DDIT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07809">
        <property type="entry name" value="RTP801_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="223" mass="24830" checksum="61AA9EEA6B6F4BAC" modified="2019-05-08" version="1">MPGVWDSFSSALPPIPEQRENQPSPIFSRGKSWQQEVPDRCVRAESSDCESLDSSLSCEEDAEYLDEVSLPDFDLLNDPEDELLCNNLMKLIQVSLNKANINSRRCSRLVMPSQLITQVSKELLHLAYSEPCGLRGALIDLCVEQGKGCHSVGQIAVDPNLVPTFQLTLVLRLDSRLWPKIQGLLSSGTSFTPGFSQSLKLSTGFRVIKKKLYSSEQLLIEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEY4</accession>
    <name>A0A452HEY4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HEY4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013384"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015317.1">
        <property type="protein sequence ID" value="ENSGAGP00000013384.1"/>
        <property type="gene ID" value="ENSGAGG00000010239.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021910">
        <property type="entry name" value="NGX6/PGAP6/MYMK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14319">
        <property type="entry name" value="FIVE-SPAN TRANSMEMBRANE PROTEIN M83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14319:SF7">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12036">
        <property type="entry name" value="DUF3522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="220" mass="24892" checksum="6ADDDD8DCC97BD1F" modified="2019-05-08" version="1">MGSLVAKLLLPTISSLVFLPTISIAAKRRFHMEAMVYFFTMFFVAIYHACDGPGLSVLCFMRYDILEYFSIYGTALSIWVSLMALAEFDEPKRSTFVMFGVLTIAVRIYHDRWGYGVYSGPIGTAVLVITVKWLQKMKEKRGLYPDKSVYTQQIGPGFCFGALALMLRFFFEEWDYTYVHSFYHCALAMSFVLLLPKVNKKAGNAGNPAKLDCSTLCCCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFA6</accession>
    <name>A0A452HFA6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Magnesium transporter that mediates the influx of magnesium into the mitochondrial matrix and regulates magnesium metabolism. Also permeable to calcium, sodium and potassium ions. Required for normal expression of the mitochondrial respiratory complex I subunits. May play a role in maintaining the inner mitochondrial membrane potential.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homopentamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion inner membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFA6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013484"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015437.1">
        <property type="protein sequence ID" value="ENSGAGP00000013484.1"/>
        <property type="gene ID" value="ENSGAGG00000010300.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045016">
        <property type="term" value="P:mitochondrial magnesium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000007">
        <property type="entry name" value="Magnesium transporter MRS2 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.330:FF:000003">
        <property type="entry name" value="Magnesium transporter MRS2 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF0">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="285"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="201"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="355" mass="41171" checksum="40E031B818FD0973" modified="2019-05-08" version="1">MKFDKEGNITSFERKKTELYQELGLQARDLRFQHLMSIATRNNRIIIRMEFLKAVVTPEFLLILDYRNLNMEHWLFQELASQLSGEGQLVTYSLPFEFRAIEAILQYWISNLQGRLNVLQPQIIETLEALVDPKLLSVDRSKLHILLQNGKSLSELETDLKVFKETILKILDEEELIEELCLTKWTDPQVFEESMSGIDHAEEMELLLENYYRQAEDLANEARELRVLIDDSESVIFINLDSHRNVMMRLNLQLTMGTFSLSLFGLIGVAFGMNLESSFEEDHRAFWLVTGIMFLGSGLIWRRLLSFLGRHLEPSLPPQVPTLLKKTHPTAGKIEVKNDFKTEAFMSSRSTLTNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFB4</accession>
    <name>A0A452HFB4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Interleukin-17 receptor D</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFB4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013518"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015475.1">
        <property type="protein sequence ID" value="ENSGAGP00000013518.1"/>
        <property type="gene ID" value="ENSGAGG00000010327.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030368">
        <property type="term" value="F:interleukin-17 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11530:FF:000003">
        <property type="entry name" value="Interleukin-17 receptor D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039465">
        <property type="entry name" value="IL-17_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031951">
        <property type="entry name" value="IL17R_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013568">
        <property type="entry name" value="SEFIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583:SF14">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16742">
        <property type="entry name" value="IL17R_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08357">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51534">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019373300" description="Interleukin-17 receptor D" evidence="10">
        <location>
            <begin position="18"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="SEFIR" evidence="11">
        <location>
            <begin position="357"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="686"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="686"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="740" mass="83988" checksum="7CC987EEC077313D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGD5</accession>
    <name>A0A452HGD5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HGD5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013922"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015941.1">
        <property type="protein sequence ID" value="ENSGAGP00000013922.1"/>
        <property type="gene ID" value="ENSGAGG00000010594.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000629">
        <property type="entry name" value="Titin b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000714">
        <property type="entry name" value="Titin novex-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015129">
        <property type="entry name" value="Titin_Z_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF4">
        <property type="entry name" value="MYOPALLADIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09042">
        <property type="entry name" value="Titin_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="6"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="104"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="627" mass="68181" checksum="E67B3BC0D24C12D8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIL7</accession>
    <name>A0A452HIL7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">A-kinase anchor protein 7 RI-RII subunit-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HIL7"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016846.1">
        <property type="protein sequence ID" value="ENSGAGP00000014738.1"/>
        <property type="gene ID" value="ENSGAGG00000011173.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="67" mass="7552" checksum="2D999C7D68437AB4" modified="2019-05-08" version="1">MVTNCEKSGNEQEEVALQNASKQIVQTAILQAVQQVSQESQQKEKRVNSSVSLQLERGELTKKHEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKL7</accession>
    <name>A0A452HKL7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Male-enhanced antigen 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May play an important role in spermatogenesis and/or testis development.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HKL7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015456"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017656.1">
        <property type="protein sequence ID" value="ENSGAGP00000015456.1"/>
        <property type="gene ID" value="ENSGAGG00000011669.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009685">
        <property type="entry name" value="MEA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17005">
        <property type="entry name" value="MALE-ENHANCED ANTIGEN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06910">
        <property type="entry name" value="MEA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0744">Spermatogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="181" mass="19099" checksum="126ADEF9BC58A874" modified="2019-05-08" version="1">MATELVAARTMGPERICPNEHEELGPQEVPEGAVDPPGDWSSEEPEEEEEGGSSGNGYFYQPLSQDPELGSWDQGSLAAEGTQGPAETAPGIQERLQMLRLHLPDPPVDSEEEEDDNGAAAQSSRSSIPMDPGQRIPLTLFYPEVAFVGDREGGSSLLLGIVSAPGPGCLEVEQSSQSPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKY0</accession>
    <name>A0A452HKY0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDV3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HKY0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015581"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017797.1">
        <property type="protein sequence ID" value="ENSGAGP00000015581.1"/>
        <property type="gene ID" value="ENSGAGG00000011751.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026806">
        <property type="entry name" value="CDV3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16284">
        <property type="entry name" value="PROTEIN CDV3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16284:SF13">
        <property type="entry name" value="PROTEIN CDV3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15359">
        <property type="entry name" value="CDV3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="156" mass="17347" checksum="5FB708B947DB1738" modified="2019-05-08" version="1">MSRSFLVLGLKDLQIDEKEDDEGEKREEPGDNWEEAGGGVEKASGPWNKTAPVQAPVAETIVTETPEPVQSSGVYRPPGARETRTRKAPQGPPEIYSDTQFPSLQSTAKHADSRKDKEMEKSFEVVKHKNRGRDEVSKNQALKLQLDNQYAVLGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLZ9</accession>
    <name>A0A452HLZ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Ephrin type-B receptor 2</fullName>
            <ecNumber evidence="4">2.7.10.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">EPH-like kinase 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452HLZ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015955"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018230.1">
        <property type="protein sequence ID" value="ENSGAGP00000015955.1"/>
        <property type="gene ID" value="ENSGAGG00000012008.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10477">
        <property type="entry name" value="EphR_LBD_B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05065">
        <property type="entry name" value="PTKc_EphR_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00185">
        <property type="entry name" value="TNFRSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000041">
        <property type="entry name" value="ephrin type-A receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000015">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000004">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000110">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1770">
        <property type="entry name" value="ephrin a2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034238">
        <property type="entry name" value="EphB2_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001090">
        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050449">
        <property type="entry name" value="Ephrin_rcpt_TKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016257">
        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001426">
        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF11">
        <property type="entry name" value="EPHRIN TYPE-B RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14575">
        <property type="entry name" value="EphA2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25599">
        <property type="entry name" value="Ephrin_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01404">
        <property type="entry name" value="Ephrin_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000666">
        <property type="entry name" value="TyrPK_ephrin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51550">
        <property type="entry name" value="EPH_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00790">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00791">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 26 28" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="27" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="15 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="10 26 28" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="16" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="565"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Eph LBD" evidence="33">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="32">
        <location>
            <begin position="301"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="32">
        <location>
            <begin position="412"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="30">
        <location>
            <begin position="644"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="31">
        <location>
            <begin position="936"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="25">
        <location>
            <position position="769"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <begin position="650"/>
            <end position="658"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26 28">
        <location>
            <position position="676"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="27">
        <location>
            <begin position="39"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="27">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00038546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00055849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00070277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00076438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="974" mass="108308" checksum="5CD456960B498592" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMX0</accession>
    <name>A0A452HMX0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HMX0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016313"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018620.1">
        <property type="protein sequence ID" value="ENSGAGP00000016313.1"/>
        <property type="gene ID" value="ENSGAGG00000012229.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15225">
        <property type="entry name" value="7tmA_OR10A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="40"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="318" mass="35803" checksum="B673FE6A00A63638" modified="2019-05-08" version="1">MEGNVSTVTEFIFLGFSDLQHLQLFLFVLVLFIYLIILIGNSLIVAVTVTDLTLQSPMYFFLRNLSVLEICYTSVIIPKTLSNLLSERQTISFLGCATQMYFFLLFGNSECCLLAVMSYDRYVAICNPMRYLLIMSRKVTIRLAFVVWIAGNVVAFEQTVTIFMLSFHGSNKIEHFFCDVIPVLKLASTDKSLNDVINTVLTVVFIIFPFVLIIISYFCILSTILKMRSAEGRHKAFSTCSSHIITVTLFFGSCFITYMRPGSSGSVDTNRAISLLYTVITPMFNPIIYSLRNKEVKEALRKLLGSKFLAPTRPGTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HR78</accession>
    <name>A0A452HR78_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HR78"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017522"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019982.1">
        <property type="protein sequence ID" value="ENSGAGP00000017522.1"/>
        <property type="gene ID" value="ENSGAGG00000013016.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="532"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="575"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="838" mass="92855" checksum="A66A15178736EC3B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRK0</accession>
    <name>A0A452HRK0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSGAGT00000020176.1">
        <property type="protein sequence ID" value="ENSGAGP00000017683.1"/>
        <property type="gene ID" value="ENSGAGG00000013111.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010811">
        <property type="term" value="P:positive regulation of cell-substrate adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049109">
        <property type="entry name" value="TARSH/FNDC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23197:SF10">
        <property type="entry name" value="TARGET OF NESH-SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23197">
        <property type="entry name" value="TARSH-RELATED FIBRONECTIN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21731">
        <property type="entry name" value="TARSH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018980244" description="Fibronectin type-III domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="116"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="1363"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="560"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1164"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1212"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1302"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1439"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="740"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="826"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="954"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1302"/>
            <end position="1315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1598" mass="176109" checksum="2BE30412F2CBE0DF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HS22</accession>
    <name>A0A452HS22_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HS22"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017870"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020387.1">
        <property type="protein sequence ID" value="ENSGAGP00000017870.1"/>
        <property type="gene ID" value="ENSGAGG00000013258.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036149">
        <property type="term" value="P:phosphatidylinositol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF2">
        <property type="entry name" value="ACYL-COA:LYSOPHOSPHATIDYLGLYCEROL ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="2">
        <location>
            <begin position="95"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="285" mass="32850" checksum="8117EC265467EF69" modified="2019-05-08" version="1">MAVTLEQAQRVGYTLLKGLLRFGFMVANNLVAIPSYILYLIILQPVRLLDNKRFWYIEGVLFKWLLAMVASWGWWAGYTVVEWGDDVKVVSEDEAMMLVNHQATGDVCTLMMCLQDKGTVVRQMMWLMDHIFKYTNFGIVSLIHGDFFIRQGKAHRDQQLVLLKEHLDKHYRSRDRKWIVLFPEGGFLRKRRETSQAFAKKNNLPFLTHVTLPRLGATQVILKSLVAQQENGIPAGGDAMVAESKSKGLQWVIDTTIAYPNAEPIDIQTWILGYRRPTVTHVHYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTC7</accession>
    <name>A0A452HTC7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="3">PPIase</shortName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 3">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452HTC7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018348"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020910.1">
        <property type="protein sequence ID" value="ENSGAGP00000018347.1"/>
        <property type="gene ID" value="ENSGAGG00000013587.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000020911.1">
        <property type="protein sequence ID" value="ENSGAGP00000018348.1"/>
        <property type="gene ID" value="ENSGAGG00000013587.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000024">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="4">
        <location>
            <begin position="128"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00058445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="294" mass="33300" checksum="94F1AA839B876B22" modified="2019-05-08" version="1">MGPLQLQQLVVVGLLKEPVFHMAKCTAEALRLNFPNKIAEPVVLPLLEFAWHEYLQEKKKELKGETWEYPSSVMCFIDGQLLGSEQELLTWAYDKWNYQDFKPVALYQAITEDFCTKHMQNSKHMFVYLDIAIQEQPTGTLLFELYSDMCPKTCANFRSLCTGEAGTSHSGMELTYKESVFHRLVKNGWIQGGDIAAGRGDGGESIYGPTFEDENFSIPHNKRGILGMANKGRHSNGSQFYITLQPAPYMDKKYVAFGQLIEGTEVLQKLEDVSTYNERPVVECKIINCGVFVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTQ9</accession>
    <name>A0A452HTQ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neuropeptide FF receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">G-protein coupled receptor 147</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">RFamide-related peptide receptor OT7T022</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HTQ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018514"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021103.1">
        <property type="protein sequence ID" value="ENSGAGP00000018514.1"/>
        <property type="gene ID" value="ENSGAGG00000013703.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15981">
        <property type="entry name" value="7tmA_NPFFR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000153">
        <property type="entry name" value="Neuropeptide FF receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005395">
        <property type="entry name" value="NPFF_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005396">
        <property type="entry name" value="NPFF_rcpt_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF4">
        <property type="entry name" value="NEUROPEPTIDE FF RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01570">
        <property type="entry name" value="NPFFRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01571">
        <property type="entry name" value="NPFFRECEPTR1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="307"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="59"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="436" mass="49728" checksum="7B1DE72DC9276824" modified="2019-05-08" version="1">MGGDTFLPSNSSSPYSKNDSRNRIFTEKYTFSSYYQHSSPIAAMFILAYIFIFLMCMIGNMLVGVIVLKNRQMRTVTNMFILNLAISDLLVGIFCMPTTLVDNLITGWPFDNAMCKMSGLVQGMSVSSSVFTLVAIAVERFRCIVYPFRQKLTLRKALISIVIIWVLALIIMCPSAVTLTVIRDEYHFMVDAYNNSYPLYSCWEAWPDKGMRKIYTTVLFSHIYVAPLTLIVIMYARIAFKLFKSSAPIGGSQSEENEGKRVSKRKVKVINMLIIVALFFTLSWLPLWTLMLLTDYGNLTEYQLNVITVYIFPFAHWLAFFNSSINPIIYGYFNENFRRGFQEAFKVQLCYVENKHKDTYSERNNIGFLFGARNRIFVEAHPSDSVQPSQSGQSNLSRIGLFPLRNGRIAHHGLMVEDLDNTGCSHNSVSVPAWDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HU48</accession>
    <name>A0A452HU48_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the myelin proteolipid protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HU48"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018630"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021238.1">
        <property type="protein sequence ID" value="ENSGAGP00000018630.1"/>
        <property type="gene ID" value="ENSGAGG00000013784.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043209">
        <property type="term" value="C:myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019911">
        <property type="term" value="F:structural constituent of myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022010">
        <property type="term" value="P:central nervous system myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001614">
        <property type="entry name" value="Myelin_PLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018237">
        <property type="entry name" value="Myelin_PLP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11683">
        <property type="entry name" value="MYELIN PROTEOLIPID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11683:SF11">
        <property type="entry name" value="MYELIN PROTEOLIPID PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01275">
        <property type="entry name" value="Myelin_PLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00214">
        <property type="entry name" value="MYELINPLP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00002">
        <property type="entry name" value="PLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00575">
        <property type="entry name" value="MYELIN_PLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01004">
        <property type="entry name" value="MYELIN_PLP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="277" mass="30293" checksum="FDEED21ADBFDAEF0" modified="2019-05-08" version="1">MGWLECCARCLIGAPFASLVATGLCFFGVALFCGCGHEALTGTERLIETYFSKNYQDYEYLIDVIHAFQYVIYGTASFFFLYGALLLAEGFYTTGAVRQIFGDYKTTICGKGLSATVTGGQKGRGSRGHHQAHSLERVCQCLGKWLGHPDKFVGITYALTVVWLLVFACSAVPVYIYFNTWTTCQSIAFPTKTSASIGTLCADARMYGVLPWNAFPGKVCGSNLLSICKTPEFQMTFHLFIATFVGAAATLVSLLTYMIAATYNFAVLKLMGRGTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HV64</accession>
    <name>A0A452HV64_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HV64"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019023"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021684.1">
        <property type="protein sequence ID" value="ENSGAGP00000019023.1"/>
        <property type="gene ID" value="ENSGAGG00000014076.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="341" mass="33659" checksum="EFC364372B9393CE" modified="2019-05-08" version="1">TGPAVLGTAQTQRQALGPTVPGAAQTQREMGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRETGPAVPGAAQTQRDGPCCAGRCADRETGPGPRRAGRCADTDRDGPRCAGRCVRSSQPTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVL7</accession>
    <name>A0A452HVL7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000019186"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021860.1">
        <property type="protein sequence ID" value="ENSGAGP00000019186.1"/>
        <property type="gene ID" value="ENSGAGG00000014174.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.20.50.160:FF:000002">
        <property type="entry name" value="Thioester-containing protein 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022041">
        <property type="entry name" value="Methyltransf_FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF139">
        <property type="entry name" value="C3 AND PZP-LIKE ALPHA-2-MACROGLOBULIN DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12248">
        <property type="entry name" value="Methyltransf_FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Kazal-like" evidence="10">
        <location>
            <begin position="1719"/>
            <end position="1766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1516"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1530"/>
            <end position="1546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1887" mass="210437" checksum="9268805072F39931" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWM1</accession>
    <name>A0A452HWM1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">cis-Golgi network membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HWM1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019540"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022248.1">
        <property type="protein sequence ID" value="ENSGAGP00000019540.1"/>
        <property type="gene ID" value="ENSGAGG00000014408.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="12">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="158" mass="18332" checksum="427FD0712099F47B" modified="2019-05-08" version="1">MTSRDTQSSEIFFFCPQITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HX00</accession>
    <name>A0A452HX00_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HX00"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019620"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022347.1">
        <property type="protein sequence ID" value="ENSGAGP00000019620.1"/>
        <property type="gene ID" value="ENSGAGG00000014466.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001187">
        <property type="entry name" value="Tyrosine-protein kinase ITK/TSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF631">
        <property type="entry name" value="SERINE_THREONINE_TYROSINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="182" mass="21115" checksum="1080F000F90C9B5A" modified="2019-05-08" version="1">MENGCLLNYLRQRQGRLNKGMLLSMCQDVCEGMEYLERNRFIHRDLAARNCLVNAKNIVKISDFGMTRYVLDDEYISSSGAKFPVKWSSPEVFHFNKYSSKSDVWSFGVLMWEVFTEGKMPFENKSNSEVVHEISEGYRLYQPYLASLTVYEVMYSCWHEKPDGRPAFAELLQTLTDIAETG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HX62</accession>
    <name>A0A452HX62_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the YAP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HX62"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019803"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022567.1">
        <property type="protein sequence ID" value="ENSGAGP00000019803.1"/>
        <property type="gene ID" value="ENSGAGG00000014601.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000019">
        <property type="entry name" value="Putative transcriptional coactivator YAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000012">
        <property type="entry name" value="transcriptional coactivator YAP1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053819">
        <property type="entry name" value="TEADIR3_omega_loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051583">
        <property type="entry name" value="YAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17616:SF9">
        <property type="entry name" value="TRANSCRIPTIONAL COACTIVATOR YAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17616">
        <property type="entry name" value="YES-ASSOCIATED PROTEIN YAP1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15238">
        <property type="entry name" value="TEADIR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WW" evidence="16">
        <location>
            <begin position="158"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="16">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="78"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="262"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="341"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="341"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="362"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="386"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="490" mass="52975" checksum="BC6116DDA4F00D1C" modified="2019-05-08" version="1">MDPGQPQQQAQPPAQQQAPPGAPSGAGQTPVAGPPPAGHQIVHVRGDSETDLEALFNAVMNPKGANVPHTLPMRLRKLPDSFFKPPEPKAHSRQASTDAGSAGALTPQHVRAHSSPASLQLGAVSPGTLTPSGVVTGPGASPSSQHLRQSSFEIPDDVPLPPGWEMAKTPSGQRYFLNHIDQTTTWQDPRKAILSQMNVTAPTSPPVQQNIMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGGSSNQQQQMRLQQLQMEKERLRLKHQELLRQALRNISPSTANSPKRQELALRSQLPTMEQDGGSQNPVSSPGMAQDLRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDISQSNIPSHQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXY2</accession>
    <name>A0A452HXY2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ribonuclease P</fullName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452HXY2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020055"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022844.1">
        <property type="protein sequence ID" value="ENSGAGP00000020055.1"/>
        <property type="gene ID" value="ENSGAGG00000014779.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030678">
        <property type="term" value="C:mitochondrial ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097745">
        <property type="term" value="P:mitochondrial tRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031595">
        <property type="entry name" value="PRORP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13547:SF1">
        <property type="entry name" value="MITOCHONDRIAL RIBONUCLEASE P CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16953">
        <property type="entry name" value="PRORP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PRORP" evidence="15">
        <location>
            <begin position="327"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="36"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="386" mass="43604" checksum="BB19942D0A8D338D" modified="2019-05-08" version="1">MFWPVPRCQQLFAVLRLLNRKHVKCFPFTTTSQSAIPMDPKATHMLSTETKSKGRDGEEKEGRARGDTSSPHPFSLFSAGAAKRRSKNQEELLMISKKNGICPPEKPLCVAEWEELKGEFKGSSKFEDVMLEQMINCNSSIDVAKSLLSMVAMRKGDISYNLLVKYLALCVRQEQMAEVYDVYDIMKVRFKTLETGAYSLLIKGLSGSNRWRETLLLLQEIKKVMTPSKGNYGDCIKGALNNQEINLACELYHEMLAKDLIPNLDTLQVFFDTGKCVKDDQLKSELLGILLYLRENQLYPGEALMQSIKLWFESIPGEKWEGHLTAIKNSGKCPVCNQNLEAIHLSQEEYNALKEKIIKDVIQGTDTFRKTSPQVGFKGLPLTGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HY14</accession>
    <name>A0A452HY14_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the copine family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HY14"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020046"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022834.1">
        <property type="protein sequence ID" value="ENSGAGP00000020046.1"/>
        <property type="gene ID" value="ENSGAGG00000014773.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000063">
        <property type="entry name" value="Copine 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF5">
        <property type="entry name" value="COPINE-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="121"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="531" mass="59246" checksum="4647783EADC9069D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYY8</accession>
    <name>A0A452HYY8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP synthase subunit gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Subunit gamma, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. With the central stalk subunit delta, is essential for the biogenesis of F(1) catalytic part of the ATP synthase complex namely in the formation of F1 assembly intermediate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the ATP synthase complex composed at least of ATP5F1A/subunit alpha, ATP5F1B/subunit beta, ATP5MC1/subunit c (homooctomer), MT-ATP6/subunit a, MT-ATP8/subunit 8, ATP5ME/subunit e, ATP5MF/subunit f, ATP5MG/subunit g, ATP5MK/subunit k, ATP5MJ/subunit j, ATP5F1C/subunit gamma, ATP5F1D/subunit delta, ATP5F1E/subunit epsilon, ATP5PF/subunit F6, ATP5PB/subunit b, ATP5PD/subunit d, ATP5PO/subunit OSCP. ATP synthase complex consists of a soluble F(1) head domain (subunits alpha(3) and beta(3)) - the catalytic core - and a membrane F(0) domain - the membrane proton channel (subunits c, a, 8, e, f, g, k and j). These two domains are linked by a central stalk (subunits gamma, delta, and epsilon) rotating inside the F1 region and a stationary peripheral stalk (subunits F6, b, d, and OSCP). Interacts with FLVCR2; this interaction occurs in the absence of heme and is disrupted upon heme binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYY8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020381"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023222.1">
        <property type="protein sequence ID" value="ENSGAGP00000020381.1"/>
        <property type="gene ID" value="ENSGAGG00000015019.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.80:FF:000007">
        <property type="entry name" value="ATP synthase gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.10:FF:000003">
        <property type="entry name" value="ATP synthase subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.80:FF:000013">
        <property type="entry name" value="ATP synthase subunit gamma, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.80">
        <property type="entry name" value="ATP synthase, gamma subunit, helix hairpin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="ATP SYNTHASE GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693:SF22">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039089">
        <property type="entry name" value="ATP_synthase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="ATP synthase (F1-ATPase), gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 15" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11 15" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5 15" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00065354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="294" mass="32514" checksum="7F1152818A202C33" modified="2019-05-08" version="1">MFSRGAAVLLYQPQWGQVRNMATLKDITRRLKSIKNIQKITKSMKMVAAAKYARAERELKPARVYGVGSLALYEKAEIKVPEDKKKHLIIGVSSDRGLCGAIHTSVAKAIKTEISTLSDAGKEVMVVGVGDKLRGLLQRTHSNHFLVTFKDVGRKPPSFGDASVIALELLNSGYEFDEGSVIYNRFRSVISYKTDEKPFYSLDTVAGAESISIYDDIDADVLRNYQEFTLANILFYSLKESTTSEQSARMTAMDNASKNASEIIDKLTLTFNRTRQAVITKELIEIISGAAALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZN9</accession>
    <name>A0A452HZN9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Myosin-binding protein C, cardiac-type</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">C-protein, cardiac muscle isoform</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the immunoglobulin superfamily. MyBP family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000020709"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023588.1">
        <property type="protein sequence ID" value="ENSGAGP00000020709.1"/>
        <property type="gene ID" value="ENSGAGG00000014743.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032036">
        <property type="term" value="F:myosin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055010">
        <property type="term" value="P:ventricular cardiac muscle tissue morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05894">
        <property type="entry name" value="Ig_C5_MyBP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000225">
        <property type="entry name" value="Myosin-binding protein C, cardiac-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000326">
        <property type="entry name" value="Myosin-binding protein C, cardiac-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000518">
        <property type="entry name" value="Myosin-binding protein C, cardiac-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000576">
        <property type="entry name" value="Myosin-binding protein C, cardiac-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000060">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000062">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000081">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000111">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040849">
        <property type="entry name" value="MyBP-C_THB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF20">
        <property type="entry name" value="MYOSIN-BINDING PROTEIN C, CARDIAC-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18362">
        <property type="entry name" value="THB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0787">Thick filament</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019405654" description="Myosin-binding protein C, cardiac-type" evidence="11">
        <location>
            <begin position="20"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="161"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="510"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="816"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="911"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1013"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="1110"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1223"/>
            <end position="1307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1324" mass="149013" checksum="38F1BC9427ECA0E4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I010</accession>
    <name>A0A452I010_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I010"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020766"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023652.1">
        <property type="protein sequence ID" value="ENSGAGP00000020766.1"/>
        <property type="gene ID" value="ENSGAGG00000015284.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050728">
        <property type="term" value="P:negative regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07160">
        <property type="entry name" value="NR_DBD_LXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06954">
        <property type="entry name" value="NR_LBD_LXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000014">
        <property type="entry name" value="Oxysterols receptor LXR-alpha isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000017">
        <property type="entry name" value="Oxysterols receptor LXR-alpha isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023257">
        <property type="entry name" value="Liver_X_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF259">
        <property type="entry name" value="OXYSTEROLS RECEPTOR LXR-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02034">
        <property type="entry name" value="LIVERXRECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="9 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="16">
        <location>
            <begin position="57"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="17">
        <location>
            <begin position="171"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="409" mass="46944" checksum="201FBB623F38DFF5" modified="2019-05-08" version="1">MGPTQLAAQDHGKRVASVFEMEEEGFSLFHGSEVPLEHAENPPLKRKKGPAPKMLGNEVCSVCGDKASGFHYNVLSCEGCKGFFRRSVIKGAQYVCKNGGKCEMDMYMRRKCQECRLRKCQEAGMREQCVLSEEQIRLKKLKKQEDDQARTVVVRPNPPSPPNTPYKLTPEQLGMIEKLVAAQQQCNQRSFTDRLKVTPWPQASDPHNREARQQRFAHFTELAIISVQEIVDFAKQLPGFLELTREDQIALLKTSTIEVMLLETSRRYNPETESITFLKDLSYNRDDFAKAGLQFEFINPIFEFSKGMNDLQLNDAEYALLIAINIFSADRPNVQDQSLVERLQHTYVEALHSYICIHRPNDHLMFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I083</accession>
    <name>A0A452I083_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP synthase F(0) complex subunit a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">F-ATPase protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Proton-conducting channel, ATP synthase F(0) complex subunit a</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>H(+)(in) = H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34979"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the ATP synthase complex composed at least of ATP5F1A/subunit alpha, ATP5F1B/subunit beta, ATP5MC1/subunit c (homooctomer), MT-ATP6/subunit a, MT-ATP8/subunit 8, ATP5ME/subunit e, ATP5MF/subunit f, ATP5MG/subunit g, ATP5MK/subunit k, ATP5MJ/subunit j, ATP5F1C/subunit gamma, ATP5F1D/subunit delta, ATP5F1E/subunit epsilon, ATP5PF/subunit F6, ATP5PB/subunit b, ATP5PD/subunit d, ATP5PO/subunit OSCP. ATP synthase complex consists of a soluble F(1) head domain (subunits alpha(3) and beta(3)) - the catalytic core - and a membrane F(0) domain - the membrane proton channel (subunits c, a, 8, e, f, g, k and j). These two domains are linked by a central stalk (subunits gamma, delta, and epsilon) rotating inside the F1 region and a stationary peripheral stalk (subunits F6, b, d, and OSCP). Interacts with DNAJC30; interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase A chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I083"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020864"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023762.1">
        <property type="protein sequence ID" value="ENSGAGP00000020864.1"/>
        <property type="gene ID" value="ENSGAGG00000015346.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00310">
        <property type="entry name" value="ATP-synt_Fo_a_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.220">
        <property type="entry name" value="ATP synthase, F0 complex, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000568">
        <property type="entry name" value="ATP_synth_F0_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023011">
        <property type="entry name" value="ATP_synth_F0_asu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045083">
        <property type="entry name" value="ATP_synth_F0_asu_bact/mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035908">
        <property type="entry name" value="F0_ATP_A_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11410">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11410:SF0">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00119">
        <property type="entry name" value="ATP-synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00123">
        <property type="entry name" value="ATPASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81336">
        <property type="entry name" value="F1F0 ATP synthase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00449">
        <property type="entry name" value="ATPASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="87" mass="9529" checksum="24F2F03475D89C46" modified="2019-05-08" version="1">ILIIIETIRLFIRPLALGVRLTANLTAGHLLIQLTSTAVLALLPTIITLSILTIIILLLLTILELAVAIIQAYVFVLLISLYLQENT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I189</accession>
    <name>A0A452I189_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ferritin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the ferritin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I189"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021262"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024214.1">
        <property type="protein sequence ID" value="ENSGAGP00000021262.1"/>
        <property type="gene ID" value="ENSGAGG00000015611.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01056">
        <property type="entry name" value="Euk_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000016">
        <property type="entry name" value="Ferritin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014034">
        <property type="entry name" value="Ferritin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF37">
        <property type="entry name" value="FERRITIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00540">
        <property type="entry name" value="FERRITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00204">
        <property type="entry name" value="FERRITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="2 8" id="KW-0409">Iron storage</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="9">
        <location>
            <begin position="10"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601519-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="182" mass="21244" checksum="8A1C21CD772DA7B8" modified="2019-05-08" version="1">MSSSPSQVRQNYNQDCEAAVNRQINLELYASYVYLSMSFYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDRDDWDNGLTAMECALHLEKNVNQSLLDLHKLATDKNDPHLCDFIETHYLDEQVKAIKQLGDYVTNLRKMGAPKYGMAEYLFDKHTLGDSDNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I247</accession>
    <name>A0A452I247_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000021559"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024552.1">
        <property type="protein sequence ID" value="ENSGAGP00000021559.1"/>
        <property type="gene ID" value="ENSGAGG00000015714.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059141">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021717">
        <property type="entry name" value="Nucleoporin_Nup160"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056547">
        <property type="entry name" value="NUP160_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056536">
        <property type="entry name" value="TPR_NUP160_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056535">
        <property type="entry name" value="TPR_NUP160_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21286">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21286:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11715">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23345">
        <property type="entry name" value="NUP160_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23354">
        <property type="entry name" value="TPR_NUP160_120_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23347">
        <property type="entry name" value="TPR_Nup160_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup120/160 beta-propeller" evidence="5">
        <location>
            <begin position="107"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 helical" evidence="6">
        <location>
            <begin position="602"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 middle TPR" evidence="8">
        <location>
            <begin position="876"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 C-terminal TPR" evidence="7">
        <location>
            <begin position="1131"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1386" mass="155077" checksum="35CA859DA667C234" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2N9</accession>
    <name>A0A452I2N9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginine(in) = L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:32143"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysine(in) = L-lysine(out)</text>
            <dbReference type="Rhea" id="RHEA:70935"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-ornithine(in) = L-ornithine(out)</text>
            <dbReference type="Rhea" id="RHEA:71199"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I2N9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021775"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024800.1">
        <property type="protein sequence ID" value="ENSGAGP00000021770.1"/>
        <property type="gene ID" value="ENSGAGG00000015978.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000024806.1">
        <property type="protein sequence ID" value="ENSGAGP00000021775.1"/>
        <property type="gene ID" value="ENSGAGG00000015978.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000024">
        <property type="entry name" value="High affinity cationic amino acid transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000009">
        <property type="entry name" value="Low affinity cationic amino acid transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004755">
        <property type="entry name" value="Cat_AA_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00906">
        <property type="entry name" value="2A0303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF20">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="287"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="336"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="492"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="557"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="14">
        <location>
            <begin position="555"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="636" mass="68624" checksum="6545089CEED55621" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I390</accession>
    <name>A0A452I390_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nonsense-mediated mRNA decay factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in nonsense-mediated mRNA decay.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I390"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021984"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025048.1">
        <property type="protein sequence ID" value="ENSGAGP00000021984.1"/>
        <property type="gene ID" value="ENSGAGG00000016131.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000025052.1">
        <property type="protein sequence ID" value="ENSGAGP00000021988.1"/>
        <property type="gene ID" value="ENSGAGG00000016131.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09884">
        <property type="entry name" value="PIN_Smg5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000017">
        <property type="entry name" value="protein SMG5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018834">
        <property type="entry name" value="DNA/RNA-bd_Est1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019458">
        <property type="entry name" value="Est1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045153">
        <property type="entry name" value="Est1/Ebs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696:SF7">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696">
        <property type="entry name" value="SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10374">
        <property type="entry name" value="EST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10373">
        <property type="entry name" value="EST1_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="9">
        <location>
            <begin position="891"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="410"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="566"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="645"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="850"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="422"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="455"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="505"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="569"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="655"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1052" mass="118342" checksum="39AFDCD41A1D868A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3G4</accession>
    <name>A0A452I3G4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I3G4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025154.1">
        <property type="protein sequence ID" value="ENSGAGP00000022069.1"/>
        <property type="gene ID" value="ENSGAGG00000016205.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="109" mass="11417" checksum="1E7A6BA21A602DEA" modified="2019-05-08" version="1">MQPTLRAVRACRPQCTLRSSSSTCAATCPANATRSRSAAGSTGGMGLAAGALSWIFRCRLLATKWVRRPSRRTLPSTSSAVAIVALRPPGGRAPAPHSRRAVPQCLCLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I412</accession>
    <name>A0A452I412_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I412"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022268"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025379.1">
        <property type="protein sequence ID" value="ENSGAGP00000022268.1"/>
        <property type="gene ID" value="ENSGAGG00000016357.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04980">
        <property type="entry name" value="IgV_L_kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001230">
        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="28"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="208" mass="22866" checksum="5EC39723B8FF4B38" modified="2019-05-08" version="1">MRTDTLPLETDPQLYFIWLFLQCFFSHPDASGDIVLTQTPESLAVSPGDRVTINCKASSSVSSYMAWYQQKSGQAPKILIYSASTRPSGVPDRFSGSGSGTDFTFTISRVEADNAGDYYCQQAQSLPLTVIQSNTKTSFLKLKYLSNCCCLKEMDQNSSIAAFDKNEKTLNETGILRGVTNIYSGPHAAVSNNITSEVNRKFCSDTDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6T6</accession>
    <name>A0A452I6T6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription factor E2F7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the E2F/DP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I6T6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023333"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026578.1">
        <property type="protein sequence ID" value="ENSGAGP00000023333.1"/>
        <property type="gene ID" value="ENSGAGG00000017103.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045935">
        <property type="term" value="P:positive regulation of nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000073">
        <property type="entry name" value="E2F transcription factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000100">
        <property type="entry name" value="E2F transcription factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015633">
        <property type="entry name" value="E2F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12081">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12081:SF25">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2F7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="13">
        <location>
            <begin position="143"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="13">
        <location>
            <begin position="282"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="19"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="575"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="678"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="901"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="697"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="916"/>
            <end position="937"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="937" mass="102830" checksum="47BF9599B64CB871" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6U1</accession>
    <name>A0A452I6U1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Syntaxin binding protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I6U1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023338"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026585.1">
        <property type="protein sequence ID" value="ENSGAGP00000023338.1"/>
        <property type="gene ID" value="ENSGAGG00000017109.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2060:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.830.10:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="588" mass="66690" checksum="9545AE8E1854F096" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7Q7</accession>
    <name>A0A452I7Q7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">CCR4-NOT transcription complex subunit 4</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">CCR4-associated factor 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">E3 ubiquitin-protein ligase CNOT4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Potential transcriptional repressor NOT4Hp</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">RING-type E3 ubiquitin transferase CNOT4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins. Involved in activation of the JAK/STAT pathway. Catalyzes ubiquitination of methylated RBM15. Plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA. As part of the PINK1-regulated signaling, upon mitochondria damage, ubiquitinates ABCE1 and thereby recruits autophagy receptors to the mitochondrial outer membrane to initiate mitophagy.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with CNOT1 via its C-terminus but does not stably associate with the CCR4-NOT complex. Interacts (via RING domain) with UBE2D2. Interacts with ABCE1, PINK1 and PELO.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452I7Q7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023655"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026950.1">
        <property type="protein sequence ID" value="ENSGAGP00000023655.1"/>
        <property type="gene ID" value="ENSGAGG00000017331.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16618">
        <property type="entry name" value="mRING-HC-C4C4_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12438">
        <property type="entry name" value="RRM_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000006">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000044">
        <property type="entry name" value="Putative ccr4-not transcription complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034261">
        <property type="entry name" value="CNOT4_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039780">
        <property type="entry name" value="Mot2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039515">
        <property type="entry name" value="NOT4_mRING-HC-C4C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14570">
        <property type="entry name" value="zf-RING_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16 27" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 26" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 25" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13 26" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 26" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="29">
        <location>
            <begin position="14"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="30">
        <location>
            <begin position="109"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="31">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="26">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="270"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="476"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="627"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="697"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="27">
        <location>
            <begin position="68"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="339"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="28">
        <location>
            <begin position="397"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="627"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="637"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="702"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="28">
        <location>
            <begin position="726"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="752"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00057081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00075062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00077837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00083547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00083942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="765" mass="84064" checksum="E7885C0D6318C57F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7T5</accession>
    <name>A0A452I7T5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I7T5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023592"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026880.1">
        <property type="protein sequence ID" value="ENSGAGP00000023592.1"/>
        <property type="gene ID" value="ENSGAGG00000017292.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029159">
        <property type="entry name" value="CA109-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16234:SF5">
        <property type="entry name" value="AFG2-INTERACTING RIBOSOME MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16234">
        <property type="entry name" value="SIMILAR TO HYPOTHETICAL PROTEIN FLJ20508"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15011">
        <property type="entry name" value="CA109-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="202" mass="23183" checksum="AEF7874C80CFD476" modified="2019-05-08" version="1">MSESSAILVLHQSLRKCFQAIQQQQEAWQTALTGCKPLLSSLSNLAEQMQACQKVTFAHTPLQGFPDLEEQLKYKQRCAAETLLEELGGKVADLQKVRDVVSGYVGTVFQLYEQHADVLGFEASVQRTALIPSLADMLEWLHDIERYYRHVYLESKLLLLQIRYENLPDMQTLPQSWEKVLEHNSRNMVQDTLLKVSFLETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8E9</accession>
    <name>A0A452I8E9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Fibrinogen C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I8E9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023935"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027259.1">
        <property type="protein sequence ID" value="ENSGAGP00000023935.1"/>
        <property type="gene ID" value="ENSGAGG00000017506.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003823">
        <property type="term" value="F:antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001867">
        <property type="term" value="P:complement activation, lectin pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF433">
        <property type="entry name" value="FICOLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0176">Collagen</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="6" id="KW-0430">Lectin</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="14">
        <location>
            <begin position="87"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="304" mass="34481" checksum="5232E5C151DB8AA6" modified="2019-05-08" version="1">DHNGHFWSWNLFLCLCLLLYGVAQHSAQWEVSLVGFNSKVSILQGCPGIPGATGPRGDPGNPGTQGTFTGKWELLLTVIQPIYIFQFLFQPGAKNCKELLTRGKVMSGWYTIYSRDCNAITVLCDMDTDGGGWIVFQRRMDGSVDFYHDWNSYKRGFGSRLSEFWLGNDNIHLLSSLGPQELRVDLRDFDNNYEFALFSSFKVAGETSKYMLFIGPFVNGTAGDSLTDHNGMMFTTYDQDNDRSEINCAVAYKGAWWYRECHMSNLNGLYLKGAHESYADGVNWASKKGYQYSYKMTEMKMRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9B0</accession>
    <name>A0A452I9B0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dolichol-phosphate mannosyltransferase subunit 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Stabilizer subunit of the dolichol-phosphate mannose (DPM) synthase complex; tethers catalytic subunit to the ER.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DPM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I9B0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024137"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027484.1">
        <property type="protein sequence ID" value="ENSGAGP00000024137.1"/>
        <property type="gene ID" value="ENSGAGG00000017652.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033185">
        <property type="term" value="C:dolichol-phosphate-mannose synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013174">
        <property type="entry name" value="DPM3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16433">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16433:SF0">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08285">
        <property type="entry name" value="DPM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019358572" description="Dolichol-phosphate mannosyltransferase subunit 3" evidence="9">
        <location>
            <begin position="20"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="98" mass="10861" checksum="7E3A4E69E1DE5ECA" modified="2019-05-08" version="1">MTKLVQWLCGLALLGTAWATLALDPLGLHLPLPCQQVLWPFPVYLLVAFGCYSLATIGYRLATFNDCEAAAKELQEQIREARADLSRRGLNRSGEQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBQ3</accession>
    <name>A0A452IBQ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Pyrroline-5-carboxylate reductase</fullName>
            <ecNumber evidence="14">1.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate (P5C) to L-proline using NAD(P)H. Proline is synthesized from either glutamate or ornithine; both are converted to P5C, and then to proline via pyrroline-5-carboxylate reductases (PYCRs). PYCR3 is exclusively linked to the biosynthesis of proline from ornithine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-proline + NAD(+) = (S)-1-pyrroline-5-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:14107"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:14111"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodecamer; composed of 5 homodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="14"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025191"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028680.1">
        <property type="protein sequence ID" value="ENSGAGP00000025191.1"/>
        <property type="gene ID" value="ENSGAGG00000018418.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000367">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000003">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053790">
        <property type="entry name" value="P5CR-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13 14" id="KW-0521">NADP</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 14" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="15">
        <location>
            <begin position="8"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="16">
        <location>
            <begin position="167"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="303" mass="32534" checksum="4C76F3CB8155D6F0" modified="2019-05-08" version="1">MEAREELRVGFVGAGRMAQGLAQGVVRAGRPVLLCWPFLSKPLTMSHLLGWQDLGCRTTHCNSEVLQHCTLVFLTTKPHLLPEVLQQIAPAVTRDHIIVSVAAGVTLQTLQQLLPAGTKVLRVVPNLPCVVQEGAVVFARGSCAGEQEATLLKSLLSACGLCEETPESYMDIHTGLSGSGVAYVSALALAQQGAVKMGMPSALANKIAAQTLLNQSGNRXAAKMILETGEHPAKLRNDVCTPGGTTIHALHELEKGALRATVMNAVEAATTRAHELVKDRFQSSSLVSLYPQKEQEYLWHLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDA0</accession>
    <name>A0A452IDA0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Rhomboid-related protein 2</fullName>
            <ecNumber evidence="4">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in regulated intramembrane proteolysis and the subsequent release of functional polypeptides from their membrane anchors. Known substrate: EFNB3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452IDA0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025819"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029370.1">
        <property type="protein sequence ID" value="ENSGAGP00000025819.1"/>
        <property type="gene ID" value="ENSGAGG00000018850.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000007">
        <property type="entry name" value="Rhomboid like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017213">
        <property type="entry name" value="Peptidase_S54_rhomboid_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051739">
        <property type="entry name" value="Rhomboid_IM_Serine_Proteases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840:SF6">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037470">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="17">
        <location>
            <begin position="106"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="14">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037470-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="291" mass="32705" checksum="19E424F6AD2E1216" modified="2019-05-08" version="1">MEMEKMNTDMSDEEEEKENNERNSRVCTSCDKIHESISKWMLPENARGTYLERANCIPPPLFIISISIVELAVFIDYAVWKPQKQWITLDTGVWNSPFIYRPDKREEAWRFISYMLVHAGIQHILGNLFLQLILGIPLEMVHKGHRVSLVYLAGVIGGSLASSVCDPLQALVGASGGVYALIGGYFMNVLVNFREMIPLFGVFRLLLAGIIVGTDMGFALYRRFIAPVNGPQVSFVAHIAGGLAGMSIGYVIFSCFDENFIKDPRFWISIVAYLVFLVFAVFFNIFLSPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDD8</accession>
    <name>A0A452IDD8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase RNF139</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">RING finger protein 139</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">RING-type E3 ubiquitin transferase RNF139</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Translocation in renal carcinoma on chromosome 8 protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDD8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025530"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029055.1">
        <property type="protein sequence ID" value="ENSGAGP00000025530.1"/>
        <property type="gene ID" value="ENSGAGG00000018654.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036513">
        <property type="term" value="C:Derlin-1 retrotranslocation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000166">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025754">
        <property type="entry name" value="TRC8_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF163">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13705">
        <property type="entry name" value="TRC8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="278"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="341"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="470"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="525"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="547"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="570"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="580" mass="66084" checksum="DFF571FA95E181ED" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDR1</accession>
    <name>A0A452IDR1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tyrosine-protein kinase STYK1</fullName>
            <ecNumber evidence="2">2.7.10.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Novel oncogene with kinase domain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Serine/threonine/tyrosine kinase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452IDR1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025649"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029183.1">
        <property type="protein sequence ID" value="ENSGAGP00000025649.1"/>
        <property type="gene ID" value="ENSGAGG00000018727.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000442">
        <property type="entry name" value="Serine/threonine/tyrosine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000450">
        <property type="entry name" value="Tyrosine-protein kinase STYK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF631">
        <property type="entry name" value="SERINE_THREONINE_TYROSINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="119"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00077455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="427" mass="48068" checksum="95AFA18EDA7761DF" modified="2019-05-08" version="1">MAGDIRRLRRMLLECNSSDKLCVVREYQTEVIIIPVLLVGVFVILLSVILWLRYRSWRSKQQQLLRPVAPLPAKDKNQKEHSESENTYIQLSETTVESLLRSAALTLKELEIPQDRLIPGSLELIHNGSFGGIYRAELETGNPGKTKTVILKALQDTAGPSEVKDFLGRIKFHQFLGHHDNMVELIGCCVVQLPLYMILEDVSHGDLLNFLWTCRRDVMTMDGIPYDLTERQVYKVGQQVAAALEFLQQKKLFHGDVAARNVLIQSDFTAKLCGLGLAYKTHTYGTSCVTQIVPLKWQAPERLLMKPPSIKSDIWSFGILLYEMITLGAPPFPEVPPSDILQHLQRLKIMKRPSTCQPAMYGIMKSCWRWSATDRPPPAELIKHLQTAAKSSNDQVVLQVPELVVPELYADVAGVGLGSLARDYSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDZ2</accession>
    <name>A0A452IDZ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IDZ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026108"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029695.1">
        <property type="protein sequence ID" value="ENSGAGP00000026108.1"/>
        <property type="gene ID" value="ENSGAGG00000019075.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000120">
        <property type="entry name" value="Putative serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF69">
        <property type="entry name" value="GRANZYME A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="34"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="265" mass="29125" checksum="1B59AF2942F66E74" modified="2019-05-08" version="1">MQESTAAIMGLLFTLSASATIILLVIPIDSCVDIIGGYAVTPHSRPYMALIKGKEYCGGALIKENWVLTAAHCKIRSKVVLGAHSLSKKEKEQQTFKIARPISFPCFNNQTKENDIMLLQLQQRAKINRAVKTIKIPNTDTDPKPGTKCQVAGWGITKNQERTPSDILREVNITIIDRKICNDQNHYNYNPMITMNMVCAGDENGGKDSCDGDSGGPLICNGELKGITSFGRAKKCGTPQYPGVYARLTKKHLLWIKQTIGGDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IE10</accession>
    <name>A0A452IE10_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IE10"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026042"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029618.1">
        <property type="protein sequence ID" value="ENSGAGP00000026042.1"/>
        <property type="gene ID" value="ENSGAGG00000019024.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514:SF4">
        <property type="entry name" value="NESPRIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514">
        <property type="entry name" value="PKA ANCHORING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="200" mass="23278" checksum="2645482C2ACD79D7" modified="2019-05-08" version="1">MLSEIEQKVASLLEDCRDRQPEDHAGPEKEAEALSLKLKDVKCNLEKVQVMLQDKYNEEQVKSLNTMQSPVRETFEQPRPSFEKNMVYTLYFIIHVVPYFQDLPLKPTEQNNLVDFIEFYVEKMQPWFDDSTTQKLESSSDVSIPRSEFAGPFLKDQTGDKWQYLQQELSSMMKSPLCQLADPQVCLCSFQFRCPTSSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IEC4</accession>
    <name>A0A452IEC4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Centromere protein M</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000025831"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029386.1">
        <property type="protein sequence ID" value="ENSGAGP00000025831.1"/>
        <property type="gene ID" value="ENSGAGG00000018862.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020987">
        <property type="entry name" value="Centromere_Cenp-M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34436">
        <property type="entry name" value="CENTROMERE PROTEIN M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34436:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11111">
        <property type="entry name" value="CENP-M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="320" mass="34523" checksum="3D24C203916893DC" modified="2019-05-08" version="1">MSLVKPFDKLPTLNAATILLVGTDESQQQQLAEAILKEKKTFKINIHVATSLPLPAERDHIRPRIDLIAFMINMQSKHSLRNVEASLSHMDANFFLGKVCFLITGVGRMNHCSVEMNAVRKLGNLYSSPVLFCELDVRMFNFVSGGVVQGGAGDGGQKILLFFCAALASCCAGDGTSIXCAFESLSFCAALASCCAGDGTSISLTSGLTGLYDVFFLSFSVSLRNVEASLSHMDANFFLGKVCFLITGVGRMNHCSVEMNAVRKLGNLYSSPVLFCELDSEGIRAATAQRLLRMLQICAGHMPGVSALFFSSLMKSSIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IG28</accession>
    <name>A0A452IG28_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IG28"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026427"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030039.1">
        <property type="protein sequence ID" value="ENSGAGP00000026427.1"/>
        <property type="gene ID" value="ENSGAGG00000019256.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000358">
        <property type="entry name" value="protein HEG homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000610">
        <property type="entry name" value="protein HEG homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24037">
        <property type="entry name" value="HEART DEVELOPMENT PROTEIN WITH EGF-LIKE DOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24037:SF3">
        <property type="entry name" value="PROTEIN HEG HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1075"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="815"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="855"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="33"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="197"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="490"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="515"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="552"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="213"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="342"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="552"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="565"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="586"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="619"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="824"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="843"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1208" mass="129493" checksum="DE21ED588B9B6179" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHA7</accession>
    <name>A0A452IHA7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IHA7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027338"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031050.1">
        <property type="protein sequence ID" value="ENSGAGP00000027338.1"/>
        <property type="gene ID" value="ENSGAGG00000019870.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007221">
        <property type="term" value="P:positive regulation of transcription of Notch receptor target"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046369">
        <property type="entry name" value="MAML1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15692">
        <property type="entry name" value="MASTERMIND-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15692:SF9">
        <property type="entry name" value="MASTERMIND-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="817"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="273"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="487"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="826"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="908" mass="99251" checksum="9504805468D3847D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHH5</accession>
    <name>A0A452IHH5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin--tyrosine ligase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IHH5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027257"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030962.1">
        <property type="protein sequence ID" value="ENSGAGP00000027257.1"/>
        <property type="gene ID" value="ENSGAGG00000019820.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000009">
        <property type="entry name" value="tubulin polyglutamylase TTLL5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF162">
        <property type="entry name" value="TUBULIN MONOGLUTAMYLASE TTLL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="138"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="445"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="909"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="180"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="468"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="909"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="924"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1128" mass="126913" checksum="FB53E519D3EEAE1B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHI2</accession>
    <name>A0A452IHI2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">XK-related protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the XK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IHI2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027376"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031103.1">
        <property type="protein sequence ID" value="ENSGAGP00000027376.1"/>
        <property type="gene ID" value="ENSGAGG00000019909.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902742">
        <property type="term" value="P:apoptotic process involved in development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043652">
        <property type="term" value="P:engulfment of apoptotic cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070782">
        <property type="term" value="P:phosphatidylserine exposure on apoptotic cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018629">
        <property type="entry name" value="XK-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050895">
        <property type="entry name" value="XK-related_scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16024">
        <property type="entry name" value="XK-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16024:SF8">
        <property type="entry name" value="XK-RELATED PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09815">
        <property type="entry name" value="XK-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="414" mass="46884" checksum="09D8EBB2CC7D1F54" modified="2019-05-08" version="1">MAGSGHGSGGPPLSSPHYRYLDLLFALLGAAAFLADLLADLWVASSYMQAGHCLWGGLVLALLCLSSLVMQFFSWAWYQTDDIEQDLPSGCCLALVHLLQLGYLYRCFRMLRVGWRVCRAETAPQQEQGYAVFLSHDISMLRLFETFLESTPQLTLALSIVLHTNKVELFQGLGICTAFLCVAWSLLDYHQSLRCFLLDKHELGLLSSTIYFLWNFLLICPRVLAVALFATLFPCYIGLHFLGIWAAMLLWVWLQGTAFMECPRWEWLYRATVAVILYFCWFNVVKGQTRQRSAIYHGFILVDSMLLVGSWLWYNAPLRERSYLLPVLLAALVCSVLGLLLRITYYKWLHPTLQTRCQASYDEVDTAEGLAGFRAGPAPAPVNRRMYQLSQNHFLVSLQAGQNWGNGVLDDMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIE3</accession>
    <name>A0A452IIE3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WD repeat and coiled-coil-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IIE3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027609"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031365.1">
        <property type="protein sequence ID" value="ENSGAGP00000027609.1"/>
        <property type="gene ID" value="ENSGAGG00000020065.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028041">
        <property type="entry name" value="WDCP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14897">
        <property type="entry name" value="WD REPEAT AND COILED-COIL-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14897:SF10">
        <property type="entry name" value="WD REPEAT AND COILED-COIL-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15390">
        <property type="entry name" value="WDCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
        <property type="entry name" value="Putative isomerase YbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="747"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="753"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00015683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="767" mass="84008" checksum="807DD1160955DA63" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIH1</accession>
    <name>A0A452IIH1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Lysine-specific demethylase</fullName>
            <ecNumber evidence="16">1.14.11.65</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>N(6),N(6)-dimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60188"/>
            <dbReference type="Rhea" id="RHEA-COMP:15541"/>
            <dbReference type="Rhea" id="RHEA-COMP:15546"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="1.14.11.65"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="16">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.</text>
    </comment>
    <comment type="domain">
        <text evidence="16">The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 16">Belongs to the JHDM2 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.65" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A452IIH1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027633"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031391.1">
        <property type="protein sequence ID" value="ENSGAGP00000027633.1"/>
        <property type="gene ID" value="ENSGAGG00000020083.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000031394.1">
        <property type="protein sequence ID" value="ENSGAGP00000027634.1"/>
        <property type="gene ID" value="ENSGAGG00000020083.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140683">
        <property type="term" value="F:histone H3K9me/H3K9me2 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070988">
        <property type="term" value="P:demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000004">
        <property type="entry name" value="Putative lysine-specific demethylase 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054294">
        <property type="entry name" value="DUF7030"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054503">
        <property type="entry name" value="KDM3AB_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054504">
        <property type="entry name" value="PWWP_KDM3B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF7">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22989">
        <property type="entry name" value="DUF7030"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22988">
        <property type="entry name" value="PWWP_KDM3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22987">
        <property type="entry name" value="Tudor_KDM3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="10 16" id="KW-0408">Iron</keyword>
    <keyword evidence="4 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjC" evidence="18">
        <location>
            <begin position="1069"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="245"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="370"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="245"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="317"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1270" mass="143320" checksum="C45198DF6865A22F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJ58</accession>
    <name>A0A452IJ58_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GP-PDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJ58"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027870"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031658.1">
        <property type="protein sequence ID" value="ENSGAGP00000027870.1"/>
        <property type="gene ID" value="ENSGAGG00000020249.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23344:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOINOSITOL INOSITOLPHOSPHODIESTERASE GDPD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23344">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="493"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="9">
        <location>
            <begin position="228"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="542" mass="61192" checksum="51B79613ADEFE205" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IK79</accession>
    <name>A0A452IK79_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine zipper like transcription regulator 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IK79"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028228"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032081.1">
        <property type="protein sequence ID" value="ENSGAGP00000028228.1"/>
        <property type="gene ID" value="ENSGAGG00000020511.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051568">
        <property type="entry name" value="LZTR1/Attractin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376:SF1">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12115" checksum="47D1C6D33E455849" modified="2019-05-08" version="1">MAAAGAPSGGGAGSSKVAPSVDFDHSCSDSVEYLTLNFGPFETVHRWRRLPPCDEFVGARRSKHTVVAYRDAIYVFGGDNGKTMLNDLLRFDVKDCSWCRYENISHVYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IL26</accession>
    <name>A0A452IL26_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic granule</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IL26"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028573"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032452.1">
        <property type="protein sequence ID" value="ENSGAGP00000028573.1"/>
        <property type="gene ID" value="ENSGAGG00000020698.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035196">
        <property type="term" value="P:miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000004">
        <property type="entry name" value="Pumilio homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF52">
        <property type="entry name" value="PUMILIO HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUM-HD" evidence="7">
        <location>
            <begin position="859"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="879"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="915"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="951"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="987"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="1023"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="1059"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="1095"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="1131"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="271"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="525"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="652"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="727"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="773"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="274"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="525"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="553"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="667"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="783"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1217" mass="129831" checksum="A1A35C59BAD0240A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMI8</accession>
    <name>A0A452IMI8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IMI8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029158"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033120.1">
        <property type="protein sequence ID" value="ENSGAGP00000029158.1"/>
        <property type="gene ID" value="ENSGAGG00000021098.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046642">
        <property type="term" value="P:negative regulation of alpha-beta T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002720">
        <property type="term" value="P:positive regulation of cytokine production involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000406">
        <property type="term" value="P:positive regulation of T cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000007">
        <property type="entry name" value="TNF receptor superfamily member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008063">
        <property type="entry name" value="Fas_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46838">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46838:SF1">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00020">
        <property type="entry name" value="TNFR_c6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01680">
        <property type="entry name" value="TNFACTORR6"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57586">
        <property type="entry name" value="TNF receptor-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019578593" description="TNFR-Cys domain-containing protein" evidence="3">
        <location>
            <begin position="36"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="1">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="4">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="4">
        <location>
            <begin position="73"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="1">
        <location>
            <begin position="73"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="4">
        <location>
            <begin position="116"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="1">
        <location>
            <begin position="116"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="74"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="307" mass="33383" checksum="299A1C682CE22DF7" modified="2019-05-08" version="1">MSSGGRSGQALRSVLRSMLRIFVIFLITQLPHLEALQCDPGEYEINGECCPLCSAGSRVVKHCKPSTSTTCMPCVDDTYTEHPNGLTECMRCKICEAGARLMVKEKCTYTKNTVCGCAPGDFCRHPAGGDCEMCSASTVCFPGFMVKEPGTEFRDNICEVCPDGTFSANNMSHTCQPWTKCEEEGMTEGKVGTATSDAVCVKRGLSVTGMALIIVFLVLSVPAVAGAVYFLWKRKKRKDERTPAQDIGACNLKPRCLSIYETDKVGEDELNEEVLENLIQTRKEGTDAALPVQETTKNSGTSTLHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IN94</accession>
    <name>A0A452IN94_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Kinesin-like protein KIF14</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Directly interacts with PRC1 within a complex also containing KIF4A, KIF20A and KIF23; targets to the central spindle. Directly interacts with CIT depending on the activation state of the kinase (stronger interaction with the kinase-dead form); targets to the midbody. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation. Interacts with AKT1; the interaction is detected in the plasma membrane upon INS stimulation and promotes AKT1 phosphorylation. Interacts with SVIL; at midbody during cytokinesis. Interacts with RADIL (via PDZ domain); recruits RADIL to the microtubule network restricting RADIL from interaction with activated RAP1A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000029365"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033346.1">
        <property type="protein sequence ID" value="ENSGAGP00000029365.1"/>
        <property type="gene ID" value="ENSGAGG00000021229.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22707">
        <property type="entry name" value="FHA_KIF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000020">
        <property type="entry name" value="Kinesin family member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000042">
        <property type="entry name" value="Kinesin family member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056523">
        <property type="entry name" value="4HB_KIF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF7">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23313">
        <property type="entry name" value="4HB_KIF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10 15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="19">
        <location>
            <begin position="373"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="18">
        <location>
            <begin position="797"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1552"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="688"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="901"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1573"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="459"/>
            <end position="466"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1589" mass="179368" checksum="CBB2DF57C80685F7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPH8</accession>
    <name>A0A452IPH8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neuritin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IPH8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033879.1">
        <property type="protein sequence ID" value="ENSGAGP00000029839.1"/>
        <property type="gene ID" value="ENSGAGG00000021529.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990138">
        <property type="term" value="P:neuron projection extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026144">
        <property type="entry name" value="Neuritin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15902:SF2">
        <property type="entry name" value="NEURITIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15902">
        <property type="entry name" value="NEURITIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15056">
        <property type="entry name" value="NRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019298639" evidence="9">
        <location>
            <begin position="26"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="161" mass="17662" checksum="EA5E1EAE5A99FE98" modified="2019-05-08" version="1">VFLGCSHPALHHWLTKCLTLVPCAAVAEDLVSTAGRRCDTIYKGFAECLISLGDSMDGQELDTICRSWDDFHTCANRVLSRCPDEAAAIWESLRQESRKIQFQGNLHDLCSARARPPGTVRGSAADETNKETLRGSARLPWPSLLATLPLLVLTSLLVPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQD5</accession>
    <name>A0A452IQD5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Arkadia family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452IQD5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030204"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034285.1">
        <property type="protein sequence ID" value="ENSGAGP00000030204.1"/>
        <property type="gene ID" value="ENSGAGG00000021784.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16681">
        <property type="entry name" value="RING-H2_RNF111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000058">
        <property type="entry name" value="E3 ubiquitin-protein ligase Arkadia isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029306">
        <property type="entry name" value="RNF111_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051073">
        <property type="entry name" value="ZNRF3_Arkadia_E3_ligases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16200">
        <property type="entry name" value="RING ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15303">
        <property type="entry name" value="RNF111_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="941"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="77"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="131"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="254"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="343"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="397"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="514"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="661"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="401"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="414"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="435"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="515"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="530"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="558"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="674"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="696"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="708"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="719"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="993" mass="108640" checksum="BE518AF37611FBA7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQW2</accession>
    <name>A0A452IQW2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IQW2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030379"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034477.1">
        <property type="protein sequence ID" value="ENSGAGP00000030379.1"/>
        <property type="gene ID" value="ENSGAGG00000021886.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030318">
        <property type="term" value="P:melanocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043313">
        <property type="entry name" value="LRMDA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46282">
        <property type="entry name" value="LEUCINE-RICH MELANOCYTE DIFFERENTIATION-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46282:SF2">
        <property type="entry name" value="LEUCINE-RICH MELANOCYTE DIFFERENTIATION-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="142" mass="16698" checksum="BEBF56A517A9A527" modified="2019-05-08" version="1">RSLEGLKTFSCLEELILDNNLLGNNLRLPRLPHLHTLTLNKNQISFQRNTYLESLLDHLADVVPSLEYLSLLGNMACPNELVSKDKDEDDYQRYRYFVLHKLTTLKFLDTRKVTKWEREEALLRGAFMKVVNQFKISNCNWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWE6</accession>
    <name>A0A452IWE6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E3 ubiquitin-protein ligase CHFR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RNF8</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CHFR family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the RNF8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IWE6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032360"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036685.1">
        <property type="protein sequence ID" value="ENSGAGP00000032360.1"/>
        <property type="gene ID" value="ENSGAGG00000023147.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22663">
        <property type="entry name" value="FHA_RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16535">
        <property type="entry name" value="RING-HC_RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000050">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000015">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000242">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03067">
        <property type="entry name" value="RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017335">
        <property type="entry name" value="RNF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FHA" evidence="17">
        <location>
            <begin position="13"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="380"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="114"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="257"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="465" mass="53831" checksum="087A678056CC61A7" modified="2019-05-08" version="1">PLLKWGPEPCRPVTIGRGFGLTYQLISKTCPLMISRYHCVFKQNAQGQWTVTDNKSLNGVWLNKERLDPLKAYPIGERDQIQLGVPLDNKETAEYEYEVIKEEWEKVSPFLAPRNDQLMGKPKGARTKRKLSLEESEASGAEGPSNSRSKRDRVSCDSEPLGRSCGRAELAKQPTENMDVKLPSPGPSVEEDKATVYSNPLNSEKATSLSHKDQKASNLAQSWTGLEMLRQTLGNIMKLKVKVEEKQTAVLRVKKSRKSGQKELRVMEQELRELQDQLCTEQEHQQQRVEQLERTFYEEQQQLLEKQQGEENLKEQLAQVLQEHRALMDELSRSKKDFEEIIQAKNKELEETKEEKEKARAQKEEVLSQMNDVLENELQCTICSEHFIEAVTLNCAHSFCSYCINEWMKRKVECPICRREIQSKTRSLVLDNCIDRMVENLNLEMKEHRLALIRERKGERWVDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWQ9</accession>
    <name>A0A452IWQ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IWQ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032489"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036836.1">
        <property type="protein sequence ID" value="ENSGAGP00000032489.1"/>
        <property type="gene ID" value="ENSGAGG00000023242.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050728">
        <property type="term" value="P:negative regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000117">
        <property type="entry name" value="Fibronectin type III domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052120">
        <property type="entry name" value="FNDC_type_III_4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14470">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14470:SF2">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018979866" description="Fibronectin type-III domain-containing protein" evidence="10">
        <location>
            <begin position="26"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="28"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="218" mass="24267" checksum="0F5079A70FFC27AB" modified="2019-05-08" version="1">MAPFPACLNSAVLVLFSCDLCLVRANRPPSPVNVTVTHLKANSATVSWDVPEGDVVIGYAISQQRQDGQMQRFIREVNTTNRACVLWDLAEDADYIIQVQSISLHGESQASKRVHFRTLKETDRLPSNSSNQGDITVEGLDKERQLQTGEIVIIVTVLLMWAAVIVLFCRQYDIIKDNDSNNNKEKTKPSSEHSTPERPTGGLLYSKKKSLSVNIIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J2W4</accession>
    <name>A0A452J2W4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000034658"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039234.1">
        <property type="protein sequence ID" value="ENSGAGP00000034658.1"/>
        <property type="gene ID" value="ENSGAGG00000024628.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000370">
        <property type="entry name" value="CMRF35-like molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF87">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Immunoglobulin V-set" evidence="6">
        <location>
            <begin position="6"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="175" mass="19332" checksum="82C6D17435295E0F" modified="2019-05-08" version="1">CLRAFPLCVSAHXGGSVSVQCQYNERYQNNTKYWCRGKEWLFCSIAVQIISAEAEVKQGRVSIQDNRIQCMFIVTMESLELGDQDVYWCGIEMVGLDQMFAVNVSVFPGKSFKCVTCCARVSALAHQLVCPSSTLLRTLPVQTSVLCSHQALSLDTHSKVIAFAGSKETEHALAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J418</accession>
    <name>A0A452J418_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WSCD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J418"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035193"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039849.1">
        <property type="protein sequence ID" value="ENSGAGP00000035193.1"/>
        <property type="gene ID" value="ENSGAGG00000025015.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000039851.1">
        <property type="protein sequence ID" value="ENSGAGP00000035195.1"/>
        <property type="gene ID" value="ENSGAGG00000025015.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051589">
        <property type="entry name" value="Sialate-O-sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964:SF7">
        <property type="entry name" value="SIALATE:O-SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964">
        <property type="entry name" value="WSCD FAMILY MEMBER CG9164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WSC" evidence="3">
        <location>
            <begin position="127"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="3">
        <location>
            <begin position="230"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="565" mass="64839" checksum="F8220CDD6C311020" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5J9</accession>
    <name>A0A452J5J9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit</fullName>
            <ecNumber evidence="5">2.4.1.255</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">O-GlcNAc transferase subunit p110</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">O-linked N-acetylglucosamine transferase 110 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.255" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452J5J9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035735"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040462.1">
        <property type="protein sequence ID" value="ENSGAGP00000035735.1"/>
        <property type="gene ID" value="ENSGAGG00000025371.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097363">
        <property type="term" value="F:protein O-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000013">
        <property type="entry name" value="UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000019">
        <property type="entry name" value="UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.150:FF:000001">
        <property type="entry name" value="UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11380:FF:000001">
        <property type="entry name" value="UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000012">
        <property type="entry name" value="UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037919">
        <property type="entry name" value="OGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029489">
        <property type="entry name" value="OGT/SEC/SPY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44366">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44366:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13844">
        <property type="entry name" value="Glyco_transf_41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="17" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 22" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="123"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="191"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="225"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="259"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="293"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="327"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="361"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="395"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="429"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="O-GlcNAc transferase C-terminal" evidence="23">
        <location>
            <begin position="476"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00029769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF13844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1045" mass="116848" checksum="3390DEDBE41200AD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5P7</accession>
    <name>A0A452J5P7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J5P7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035792"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040521.1">
        <property type="protein sequence ID" value="ENSGAGP00000035792.1"/>
        <property type="gene ID" value="ENSGAGG00000025403.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="92" mass="10502" checksum="51E8E8A2E496C77D" modified="2019-05-08" version="1">MAVVQCSFPVHSVWSTRRARTLAQSKWTLLGRILRSQVFAQLEVNRCIGTAPPSMVNNEQRQHAEHIFLSFRKSKSPFAVCKHILGKSSVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5Q2</accession>
    <name>A0A452J5Q2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Exportin-4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J5Q2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035790"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040519.1">
        <property type="protein sequence ID" value="ENSGAGP00000035790.1"/>
        <property type="gene ID" value="ENSGAGG00000025383.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000077">
        <property type="entry name" value="Exportin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000130">
        <property type="entry name" value="Exportin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044189">
        <property type="entry name" value="XPO4/7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596">
        <property type="entry name" value="EXPORTIN 4,7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596:SF1">
        <property type="entry name" value="EXPORTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exportin-1 C-terminal" evidence="9">
        <location>
            <begin position="991"/>
            <end position="1102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1180" mass="133770" checksum="4F39EFA67E9AA724" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J6K9</accession>
    <name>A0A452J6K9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mitochondrial transcription rescue factor 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J6K9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036136"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040906.1">
        <property type="protein sequence ID" value="ENSGAGP00000036136.1"/>
        <property type="gene ID" value="ENSGAGG00000025610.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903108">
        <property type="term" value="P:regulation of mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057896">
        <property type="entry name" value="MTRES1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13633">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION RESCUE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13633:SF3">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION RESCUE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25818">
        <property type="entry name" value="MTRES1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondrial transcription rescue factor 1 C-terminal" evidence="2">
        <location>
            <begin position="117"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="223" mass="26302" checksum="CF18840F52BA34F8" modified="2019-05-08" version="1">MTGFRLPITAFRKLNVWLGVWEKFPSNKLCVSWRRRIYSIHQSCIINYRKHFSISPVIHDVFLRPPSKCISVPCVRLKSDKSSRKKKQTLQEEEEEEERSDSEDEFENDPNIVKDYKDLDKVVQSLRYDIIMKSGLDIARNKVEDAFYNGELRLNGEKLWKKSRTVKVGDTLDLIVGEDKETETAVVMRVVFKKASEKTESEKYKVVLRRWKNLKVPKQDVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GF34</accession>
    <name>A0A452GF34_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000000121"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000142.1">
        <property type="protein sequence ID" value="ENSGAGP00000000121.1"/>
        <property type="gene ID" value="ENSGAGG00000000102.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042633">
        <property type="entry name" value="CRYZL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44461">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44461:SF1">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="18"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="380" mass="42421" checksum="B7FDF17803265E46" modified="2019-05-08" version="1">MLFQVTMKVLYCQQNSAGEEITCVFQERENLPVTRDNDVKIQVKACALSWTDIKLLSEMKMKKEFLPVGREIAGVVLEVGSKVSFFQPDDEVVGILPLDSEESGLCEVALVHEHYLVHKPEKVPWIEAAGTIRDGLRAYTALHYLSHVSPGKTVLVMXALHYLSHVSPGKTVLVMDGASPFGTIAIQLAQHRGAKVISTAYSLEDKQYLERLRPPVGMRQPLIARVIDVSNGKTDVAESCLEETGGLGVDIVLDAGVRLYSKEDESTLKPQLLPHKHDIITLLSVGGHWVTIEKNLQLDPPDSHCLFLKGATVSFLNDEVWNLSNMQQGKYLCILEDVMEKLSNSVFRPQLDEPVPLYEAKVSMEIVQKNQARKRQVIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGB5</accession>
    <name>A0A452GGB5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
            <location evidence="1 10">Extracellular space</location>
            <location evidence="1 10">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GGB5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000621"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000698.1">
        <property type="protein sequence ID" value="ENSGAGP00000000621.1"/>
        <property type="gene ID" value="ENSGAGG00000000503.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19352">
        <property type="entry name" value="Wnt_Wnt8b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013301">
        <property type="entry name" value="Wnt8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF94">
        <property type="entry name" value="PROTEIN WNT-8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01892">
        <property type="entry name" value="WNT8PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="327" mass="36193" checksum="033ECF6302C60215" modified="2025-10-08" version="2">MMTGPKAYLIYSSSVAAGAQSGIEECKFQFAWDRWNCPERALQLSSHGGLRSANRETAFVHAISSAGVMYTLTRNCSLGDFDNCGCDDSRNGQLGGQGWLWGGCSDNVGFGEAISKQFVDALETGQDARAAMNLHNNEAGRKAVKGTMKRTCKCHGVSGSCTTQTCWLQLPEFREVGTYLKEKYHKALKVDLLQGAGNSAASRGAIAETFSSISKKELVHLEDSPDYCLENKTLGLLGTEGRECLKRGKALSRWEKRSCRRLCGDCGLAVEERRAEMVSSCNCKFHWCCAVRCEQCRKRVTKYFCVRKEKRERSGGGRATRKLKRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGI1</accession>
    <name>A0A452GGI1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GGI1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000668"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000752.1">
        <property type="protein sequence ID" value="ENSGAGP00000000668.1"/>
        <property type="gene ID" value="ENSGAGG00000000528.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18451">
        <property type="entry name" value="BACK_KLHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18241">
        <property type="entry name" value="BTB_POZ_KLHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF420">
        <property type="entry name" value="KELCH-LIKE PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13964">
        <property type="entry name" value="Beta-prop_Calicin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="53"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="668" mass="76083" checksum="1C6E172D20F2F478" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GH30</accession>
    <name>A0A452GH30_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GH30"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000879"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000998.1">
        <property type="protein sequence ID" value="ENSGAGP00000000879.1"/>
        <property type="gene ID" value="ENSGAGG00000000736.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22656">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22656:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="259"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="259"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="284" mass="33144" checksum="2E7C9ED483B243A3" modified="2019-05-08" version="1">MSALPRKAMLEIVSYYRQKFLLSTGRRVWIDAGGSVEHGKGLRTLRKSPAPQRGRITPMSAFAGAARISVMNDKELEAYVETLKASSVPSDSPYAQVPIFPLLPNRDGMSIPKPKKDLQKLEMQRRKEPISSFENHFFHKRNWLREATAFKPPDYFRDKKNSLSLGPHLMERQHRLTIEDLREIRLDVKRVTEMYKQGMALKERNRMLKLWRRLHGGQIGLESGNHSFYHTFSTYSWSWNARQELVTPNELQQYDNKLYRREHSSARSDDSEVKSGIRKGKERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHS6</accession>
    <name>A0A452GHS6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FHOD1 N-terminal GTPase-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GHS6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001128"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001263.1">
        <property type="protein sequence ID" value="ENSGAGP00000001128.1"/>
        <property type="gene ID" value="ENSGAGG00000000900.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055003">
        <property type="term" value="P:cardiac myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041387">
        <property type="entry name" value="FHOD1_GBD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920:SF3">
        <property type="entry name" value="FH1_FH2 DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920">
        <property type="entry name" value="FORMIN HOMOLOGY 2 DOMAIN CONTAINING, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18382">
        <property type="entry name" value="Formin_GBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHOD1 N-terminal GTPase-binding" evidence="1">
        <location>
            <begin position="15"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="132" mass="14872" checksum="669EAC66D686B517" modified="2019-05-08" version="1">RGGGDDPGVLAALKQVQFLDDTDPFNSTNFPEPTRPPLYTFREDIALVTQLAGVHRLLRAPHKLDDCTLQLSHNGTYLDLESTLAEQRDELEGFQEDAGYNFNQTLSINGGLLNRKRFYICMQKLLCMKKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIC7</accession>
    <name>A0A452GIC7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GIC7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001334"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001494.1">
        <property type="protein sequence ID" value="ENSGAGP00000001334.1"/>
        <property type="gene ID" value="ENSGAGG00000001069.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051020">
        <property type="term" value="F:GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035024">
        <property type="term" value="P:negative regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22734">
        <property type="entry name" value="FHA_RAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15472">
        <property type="entry name" value="Myo5p-like_CBD_Rasip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17116">
        <property type="entry name" value="RA_Radil_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037983">
        <property type="entry name" value="CBD_Rasip1/Radil"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052072">
        <property type="entry name" value="Vascular_dev_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN YPR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027:SF4">
        <property type="entry name" value="RAS-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="2">
        <location>
            <begin position="133"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="3">
        <location>
            <begin position="570"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="936" mass="103278" checksum="915154E40B96F529" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIJ4</accession>
    <name>A0A452GIJ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001409"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001594.1">
        <property type="protein sequence ID" value="ENSGAGP00000001409.1"/>
        <property type="gene ID" value="ENSGAGG00000001114.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23790">
        <property type="entry name" value="UBCc_UBE2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000004">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="7">
        <location>
            <begin position="4"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="147" mass="16778" checksum="69E6ED7753C82A4D" modified="2019-05-08" version="1">MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAXTPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNDGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIV0</accession>
    <name>A0A452GIV0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GIV0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001528"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001743.1">
        <property type="protein sequence ID" value="ENSGAGP00000001528.1"/>
        <property type="gene ID" value="ENSGAGG00000001236.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044599">
        <property type="term" value="C:AP-5 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028222">
        <property type="entry name" value="AP5Z1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055450">
        <property type="entry name" value="AP5Z1_ARM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056856">
        <property type="entry name" value="TPR_AP5Z1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056857">
        <property type="entry name" value="TPR_AP5Z1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46488">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT ZETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46488:SF1">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT ZETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14764">
        <property type="entry name" value="SPG48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25153">
        <property type="entry name" value="TPR_AP5Z1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25154">
        <property type="entry name" value="TPR_AP5Z1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP-5 complex subunit zeta-1 N-terminal TPR" evidence="2">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="AP-5 complex subunit zeta-1 ARM repeats" evidence="1">
        <location>
            <begin position="321"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="AP-5 complex subunit zeta-1 C-terminal TPR" evidence="3">
        <location>
            <begin position="450"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="811" mass="90997" checksum="F7185FD018E72BCD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIY1</accession>
    <name>A0A452GIY1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gastrin-releasing peptide</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the bombesin/neuromedin-B/ranatensin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GIY1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001618"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001838.1">
        <property type="protein sequence ID" value="ENSGAGP00000001618.1"/>
        <property type="gene ID" value="ENSGAGG00000001289.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000874">
        <property type="entry name" value="Bombesin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16866">
        <property type="entry name" value="GASTRIN-RELEASING PEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16866:SF2">
        <property type="entry name" value="GASTRIN-RELEASING PEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02044">
        <property type="entry name" value="Bombesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00257">
        <property type="entry name" value="BOMBESIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0027">Amidation</keyword>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019079055" description="Gastrin-releasing peptide" evidence="10">
        <location>
            <begin position="29"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="156" mass="17587" checksum="2B5543C9D4E9DDFA" modified="2019-05-08" version="1">MRGGEMPRSLLSLGLFALVLLEVHWGAAAPLQANGGASPLAKIYPRGSHWAVGHLMGKKSTGDFPYVYEEENKIPFSALPENVKQLGEYLQWEETLKNLLRLLEGNENRSTQILREELPWYTKNTWETDDDSSFKDVSLIQEGTVNLNLKKKQISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJM2</accession>
    <name>A0A452GJM2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">TGF-beta family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GJM2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001874"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002132.1">
        <property type="protein sequence ID" value="ENSGAGP00000001874.1"/>
        <property type="gene ID" value="ENSGAGG00000001503.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13766">
        <property type="entry name" value="TGF_beta_GDF5_6_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000001">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF43">
        <property type="entry name" value="GROWTH_DIFFERENTIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00669">
        <property type="entry name" value="INHIBINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0339">Growth factor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="13">
        <location>
            <begin position="230"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="29"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="351" mass="38525" checksum="64ECD8F8B83EF964" modified="2019-05-08" version="1">TFPRKENRFLPCPSAVLLPGRPSAVLLPGWKPGKSVVRGSHPGGRSPAESRPHRSSPFPLLPWEGAPGSPGRGRGGRFGAGRAAGASLPCLPSAAGRSPGVKLTVIMCYLADDLSPAPLRRQKYLFDVSTLSDKEELVGAELRLFRKAPGHRLLQPPPGLSSMQLFPCLSPRLLGARTLAPREAPPAGWEVFDVWQGLQQQQPWKQLCLELRAPGGRGQLPLPDLRSLGFGRRGRAQQEKALLRKSRLRCSKKPLHVNFKELGWDDWIIAPLEYEAYHCEGVCDFPLRSHLEPTNHAIIQTLMNSMDPSSTPPSCCVPTKLTPISILYIDAGNNVVYKQYEDMVVESCGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLM4</accession>
    <name>A0A452GLM4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GLM4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002521"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002882.1">
        <property type="protein sequence ID" value="ENSGAGP00000002521.1"/>
        <property type="gene ID" value="ENSGAGG00000002027.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002014">
        <property type="entry name" value="Ig kappa chain C region, A allele"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="9"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="122" mass="13233" checksum="1BC99403B86C721F" modified="2019-05-08" version="1">PDMDSWALPSLSSELGDIVMTQTPESLAVSPGDTVTIRCKASSSLSSGSYHYLAWYQQKSGQAPKLLIYLASTLQSGVPARFSGSGSGTDYTLTISRVEAEDAGDYYCQQRYDTRHMIPLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLR4</accession>
    <name>A0A452GLR4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GLR4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002725"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003120.1">
        <property type="protein sequence ID" value="ENSGAGP00000002725.1"/>
        <property type="gene ID" value="ENSGAGG00000002187.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000703">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000739">
        <property type="entry name" value="Zgc:171418 protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000506">
        <property type="entry name" value="Zinc finger protein 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000247">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000620">
        <property type="entry name" value="Zinc finger protein 263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="324" mass="36482" checksum="CC3DB2C4B19EE2E2" modified="2019-05-08" version="1">RAHKCLDCGKSFFTKSALVKHQAIHIGEKPHKCLDCGKSFTDRTNLVNHQAIHTGLRAHKCLDCGKSYFRKSALVKHQAIHIGEKPHKCLDCGKSFTDKSNLVNHQAIHTGLRAHKCLDCGKSYFRKSALVKHQAIHIGEKPHKCLDCGKSFTDKSSLVRHRALHTGERPHKCLECGKSFIQRSHLVYHQALHTGKRPHKCLDCGKSFTDKSTLVKHQAIHTGEKPHKCLDCGKSFTERSNLVKHQVIHTGVRAHKCLDCGKSFFTKSALVKHQAIHIGEKPHKCLDCGKSFTDRSSLVRHQAIHTGARPHKLLGVWENFHKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GM61</accession>
    <name>A0A452GM61_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GM61"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002912"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003323.1">
        <property type="protein sequence ID" value="ENSGAGP00000002905.1"/>
        <property type="gene ID" value="ENSGAGG00000002320.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000003327.1">
        <property type="protein sequence ID" value="ENSGAGP00000002908.1"/>
        <property type="gene ID" value="ENSGAGG00000002320.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000003332.1">
        <property type="protein sequence ID" value="ENSGAGP00000002912.1"/>
        <property type="gene ID" value="ENSGAGG00000002320.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001038">
        <property type="entry name" value="Zinc finger protein 217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001201">
        <property type="entry name" value="Zinc finger protein 217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001687">
        <property type="entry name" value="Zinc finger protein 217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051967">
        <property type="entry name" value="Krueppel_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925:SF4">
        <property type="entry name" value="ZINC FINGER PROTEIN 217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="487"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="515"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="308"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="619"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="799"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="833"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="334"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="382"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="453"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="627"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="799"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="834"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1079" mass="120417" checksum="94D9DF00AD538C6F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GME4</accession>
    <name>A0A452GME4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">E3 ubiquitin-protein ligase SH3RF2</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Protein phosphatase 1 regulatory subunit 39</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">RING finger protein 158</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">RING-type E3 ubiquitin transferase SH3RF2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">SH3 domain-containing RING finger protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Has E3 ubiquitin-protein ligase activity. Acts as an anti-apoptotic regulator of the JNK pathway by ubiquitinating and promoting the degradation of SH3RF1, a scaffold protein that is required for pro-apoptotic JNK activation. Facilitates TNF-alpha-mediated recruitment of adapter proteins TRADD and RIPK1 to TNFRSF1A and regulates PAK4 protein stability via inhibition of its ubiquitin-mediated proteasomal degradation. Inhibits PPP1CA phosphatase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SH3RF family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452GME4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002984"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003417.1">
        <property type="protein sequence ID" value="ENSGAGP00000002984.1"/>
        <property type="gene ID" value="ENSGAGG00000002381.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16749">
        <property type="entry name" value="RING-HC_SH3RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11932">
        <property type="entry name" value="SH3_SH3RF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11784">
        <property type="entry name" value="SH3_SH3RF2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000077">
        <property type="entry name" value="E3 ubiquitin-protein ligase SH3RF1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000173">
        <property type="entry name" value="E3 ubiquitin-protein ligase SH3RF2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000191">
        <property type="entry name" value="E3 ubiquitin-protein ligase SH3RF2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000063">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase SH3RF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028511">
        <property type="entry name" value="SH3RF2_RING-HC_Zfn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035794">
        <property type="entry name" value="SH3RF2_SH3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035822">
        <property type="entry name" value="SH3RF2_SH3_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF60">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SH3RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 24" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 23" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="27">
        <location>
            <begin position="12"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="26">
        <location>
            <begin position="126"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="26">
        <location>
            <begin position="188"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="26">
        <location>
            <begin position="386"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="260"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="501"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="564"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="611"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="501"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00056451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00080287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00080383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00082659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00084054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="744" mass="81839" checksum="FEE4C37566A592C2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMZ7</accession>
    <name>A0A452GMZ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SOGA coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMZ7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003242"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003739.1">
        <property type="protein sequence ID" value="ENSGAGP00000003242.1"/>
        <property type="gene ID" value="ENSGAGG00000002624.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049885">
        <property type="entry name" value="MTCL1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027881">
        <property type="entry name" value="SOGA_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15742">
        <property type="entry name" value="GIRDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15742:SF2">
        <property type="entry name" value="PROTEIN SOGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11365">
        <property type="entry name" value="SOGA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SOGA coiled-coil" evidence="7">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="SOGA coiled-coil" evidence="7">
        <location>
            <begin position="103"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="205"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="53"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="379" mass="43626" checksum="F2D8AF917B21D3FB" modified="2019-05-08" version="1">MRKKMAKIDKEKDRFEHELQKYRSFYGDLDSPLPKGEAGGPPTTREAELKLRLRLVEEEANILGRKIVELEVENRGLKAELDDLRGDDFSGTANPLMGEQSESLSELRQHLQLVEDETELLRRNLSDLEEQNKRITTELNKYKYKSGTHESSRHHDNAKTEALQEELKAARMQINELSGKVMQLQYENRVLMSNMQRYDLASHLGIRGSPRDSDAESDAGKKESDDDSRPPHRKREGPIGGESDSEEVRNIRCLTPTRSFYPTSTGWQKSFTDRQQMKDIRSEAERLGKTIDRLISDTSTIITEARIYVANGDLFGLMDEEDDGSRIREHELLYRINAQMKAFRKELQTFIDRLEVPNPCLLPFPHPQSSGFLKLQCCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNF7</accession>
    <name>A0A452GNF7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GNF7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003395"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003916.1">
        <property type="protein sequence ID" value="ENSGAGP00000003395.1"/>
        <property type="gene ID" value="ENSGAGG00000002746.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15912">
        <property type="entry name" value="7tmA_OR6C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047132">
        <property type="entry name" value="Olfact_rcpt_6C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454:SF18">
        <property type="entry name" value="OLFACTORY RECEPTOR 6C76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="39"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="315" mass="35508" checksum="2ACFA602FC8861BA" modified="2019-05-08" version="1">MWNQTTVTEFILIGFLYSHPLECLIAVGFLASYLLILSGNIMIITIILLDRHLHSPMHYFLWNLSCLEILITSSVLPKVIASFLTGDRSISYLACITQCYFYFLLGCSEFVLLAVMSYDRYVAICNPLRYTMVKNAQLCFQLVVGSWATGFLATIIPTILVITLPFCNANQIDHFFCDRLALVKLSCVDTSFVELLSFMTSSAILMGSLILTVVSYTCIVATIFRITSHSGRNKAFSTCSSHFIIITMGYGSCIFMYVRPSGSEISLNKLVALLNTVVTPLMSPFIFSMRNQQMKDSLRTALKRSMNFSRKHFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPW8</accession>
    <name>A0A452GPW8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPW8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003952"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004599.1">
        <property type="protein sequence ID" value="ENSGAGP00000003952.1"/>
        <property type="gene ID" value="ENSGAGG00000003233.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF231">
        <property type="entry name" value="SODIUM-DEPENDENT PROLINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="445"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="477"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="518"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="552"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="657" mass="73953" checksum="37620679F99CC04E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQM2</accession>
    <name>A0A452GQM2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQM2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004229"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004961.1">
        <property type="protein sequence ID" value="ENSGAGP00000004229.1"/>
        <property type="gene ID" value="ENSGAGG00000003505.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071398">
        <property type="term" value="P:cellular response to fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15170">
        <property type="entry name" value="7tmA_FFAR2_FFAR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013312">
        <property type="entry name" value="GPR40-rel_orph"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45822:SF5">
        <property type="entry name" value="FREE FATTY ACID RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45822">
        <property type="entry name" value="FREE FATTY ACID RECEPTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01904">
        <property type="entry name" value="GPR40FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="35"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="430" mass="47336" checksum="535AFAEEB2763D7A" modified="2019-05-08" version="1">MSPFSPVGAEKMSLNVSIPLILTIYIFTFLTGLPSNLLASYTFLVKVRQKPTPVNILLLNLTVSDIFLLIFLPFKMVEAASGMEWSLPAFLCPLTNYVYFNSIHISSLFLMAVSVDRYLGVAFPSKYKLWRHPGYAAATSVVFWVVTCAHCSFVYIVQYEVLSPNGTVASNNMSHCYEDFSPTQLQILLPVRLEFFIVLFCLPFTITIFCYVNFVRILVALPNIPARRKQRAVGLAVATLFNFIVCFTPFNLSHVVGFVQNKSPSWRVYALLFSTLNAALDPANFYFSSSAIQRAFANCLATLWYKLSTILSRCHLPCFTRCGEGGREVKVTRDRLFASSTKPSLSHAPQKQSLLPSICGAPPVSPELSCLGLVQKPGQSQPGMGGGVTVVGAWLGPSMQEGPEGARAEQALAWLIAALPRGQSDSPPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRV7</accession>
    <name>A0A452GRV7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA methyltransferase 2, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">16S rRNA (uridine(1369)-2'-O)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">16S rRNA [Um1369] 2'-O-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Protein ftsJ homolog 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methyluridine at position 1369 (Um1369) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA. This activity may require prior 2'-O-methylguanosine modification at position 1370 (Gm1370) by MRM3. Essential for late-stage assembly of mtLSU required for efficient translation of mitochondrial DNA encoded proteins; methyltransferase activity is not required for this function. Essential for mitochondrial respiratory function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>uridine(1369) in 16S rRNA + S-adenosyl-L-methionine = 2'-O-methyluridine(1369) in 16S rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47764"/>
            <dbReference type="Rhea" id="RHEA-COMP:11903"/>
            <dbReference type="Rhea" id="RHEA-COMP:11904"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GRV7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004444"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005211.1">
        <property type="protein sequence ID" value="ENSGAGP00000004444.1"/>
        <property type="gene ID" value="ENSGAGG00000003680.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902775">
        <property type="term" value="P:mitochondrial large ribosomal subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000129">
        <property type="entry name" value="Mitochondrial rRNA methyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF18">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="16">
        <location>
            <begin position="52"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR005461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="247" mass="27950" checksum="2555D1E3383D434A" modified="2019-05-08" version="1">MSRYQRLVSTSLQCQRFHTAAGRLKSKTGAEHRWLERHLKDPFVRAAKQHNYRCRSAFKLLEIDDKHKVLHPGLSVIDCGAAPGAWSQVAVQRVNALGTDPNAPVGFVLGVDLLHISPLEGAVFLPNADITDPVTLKKIQELLPTERADVILSDIAPNATGIRELDHQKLINLCLSLLDLSQSILRPRGTILCKFWDGREAHLLQNRLMEHFQDVRTIKPQSSRKESAEIYYLAKLYNHGVKHQKHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVN5</accession>
    <name>A0A452GVN5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GVN5"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006920.1">
        <property type="protein sequence ID" value="ENSGAGP00000005935.1"/>
        <property type="gene ID" value="ENSGAGG00000004824.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001239">
        <property type="entry name" value="Prot_inh_Kazal-m"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21312:SF28">
        <property type="entry name" value="OVOINHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21312">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00050">
        <property type="entry name" value="Kazal_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00290">
        <property type="entry name" value="KAZALINHBTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00282">
        <property type="entry name" value="KAZAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Kazal-like" evidence="6">
        <location>
            <begin position="26"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="93" mass="10588" checksum="D1156A36E2B8AA1F" modified="2019-05-08" version="1">MLLNFHSNNNFFVWQIDCSRYPRTKDPRKIACPRNFEPVCGTDACPRIYAPVCGTNGVTYDNECLLCAEILKGATIRLAHKGQCMVKVMAHFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GX20</accession>
    <name>A0A452GX20_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Epithelial discoidin domain-containing receptor 1</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">CD167 antigen-like family member A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Discoidin receptor tyrosine kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Tyrosine kinase DDR</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="22">Homodimer. Interacts (via PPxY motif) with WWC1 (via WW domains) in a collagen-regulated manner. Forms a tripartite complex with WWC1 and PRKCZ, but predominantly in the absence of collagen. Interacts (tyrosine phosphorylated) with SHC1. Interacts with SRC. Interacts with MYH9. Interacts with CDH1. Interacts with PTPN11. Interacts with NCK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452GX20"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007718.1">
        <property type="protein sequence ID" value="ENSGAGP00000006641.1"/>
        <property type="gene ID" value="ENSGAGG00000005364.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038062">
        <property type="term" value="F:protein tyrosine kinase collagen receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000007">
        <property type="entry name" value="Discoidin domain receptor tyrosine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000589">
        <property type="entry name" value="Discoidin domain receptor tyrosine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000053">
        <property type="entry name" value="Epithelial discoidin domain-containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1190:FF:000002">
        <property type="entry name" value="epithelial discoidin domain-containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048525">
        <property type="entry name" value="DDR1-2_DS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002011">
        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF333">
        <property type="entry name" value="EPITHELIAL DISCOIDIN DOMAIN-CONTAINING RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21114">
        <property type="entry name" value="DDR1-2_DS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00239">
        <property type="entry name" value="RECEPTOR_TYR_KIN_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="19" id="KW-0421">Lactation</keyword>
    <keyword evidence="14 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0635">Pregnancy</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 28" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="30">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="29">
        <location>
            <begin position="609"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="341"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="351"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00061639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00063678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00074969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00078085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00079442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00082942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="906" mass="101411" checksum="61DABA74D34E4F61" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYG6</accession>
    <name>A0A452GYG6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CABIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the themis family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GYG6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007103"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008225.1">
        <property type="protein sequence ID" value="ENSGAGP00000007103.1"/>
        <property type="gene ID" value="ENSGAGG00000005713.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025946">
        <property type="entry name" value="CABIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039671">
        <property type="entry name" value="THEMIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15215">
        <property type="entry name" value="CABIT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15215:SF2">
        <property type="entry name" value="PROTEIN THEMIS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12736">
        <property type="entry name" value="CABIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CABIT" evidence="3">
        <location>
            <begin position="18"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="CABIT" evidence="3">
        <location>
            <begin position="259"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="533"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="592"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="620"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="658" mass="73735" checksum="E34A82C4A10D09D4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYW8</accession>
    <name>A0A452GYW8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GDP-fucose protein O-fucosyltransferase 2</fullName>
            <ecNumber evidence="3">2.4.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Peptide-O-fucosyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + GDP-beta-L-fucose = 3-O-(alpha-L-fucosyl)-L-seryl-[protein] + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63644"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:17914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:189632"/>
            <dbReference type="EC" id="2.4.1.221"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:63645"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + GDP-beta-L-fucose = 3-O-(alpha-L-fucosyl)-L-threonyl-[protein] + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70491"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:17915"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:189631"/>
            <dbReference type="EC" id="2.4.1.221"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:70492"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyltransferase 68 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.221" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452GYW8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007249"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008381.1">
        <property type="protein sequence ID" value="ENSGAGP00000007249.1"/>
        <property type="gene ID" value="ENSGAGG00000005822.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046922">
        <property type="term" value="F:peptide-O-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045130">
        <property type="entry name" value="OFUT2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13398">
        <property type="entry name" value="GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13398:SF0">
        <property type="entry name" value="GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019081094" description="GDP-fucose protein O-fucosyltransferase 2" evidence="13">
        <location>
            <begin position="26"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="159" mass="17963" checksum="F4B9345EE1CB8A2D" modified="2019-05-08" version="1">MAARGPGRLLLLLGLAAALFPPWAAQPPPGAFQAGHGAASLPAAAAGRRRYLLYDVNPPEGFNLRRDVYIRVASLIKTLLKSEAWVLVLPPWGRLYHWQSPDIHQVRIPWSEFFDLPSLNQNIPVIEYEQFLAGKVHLFYYLGGVNKSLRGFRHRYFCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZL0</accession>
    <name>A0A452GZL0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZL0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007643"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008824.1">
        <property type="protein sequence ID" value="ENSGAGP00000007643.1"/>
        <property type="gene ID" value="ENSGAGG00000006118.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13954">
        <property type="entry name" value="7tmA_OR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047132">
        <property type="entry name" value="Olfact_rcpt_6C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454:SF73">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="294" mass="33479" checksum="26BF40C2D1BB4CC0" modified="2019-05-08" version="1">MVKGNQTNVREFILLGFAGSRYVQISLFLLFLFIYLLTLMGNITIISLVGTHSHLHTPMYFFLCNLSFLDICKTISFSSCLLQMYFVFSLGCTEYLLLAVMAYDRYLAICQPLRYNTIMNSTFSIQLALGSWASSFLTTSVLAFLITGLSFCDSTIINHFFCDVDSWIVLSCTDTRLIEMVYITICFIVVLGSCAIILVSYIYIISTVLRIPSAQAWQKAFSTCSAHLTVVVIWYGCSIFLYVKPSKQNSLEINKVVTLLNTIMTPLLNPFIYTLRNKDIKEILRKAFRKHHCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZU5</accession>
    <name>A0A452GZU5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">small monomeric GTPase</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452GZU5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007804"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008995.1">
        <property type="protein sequence ID" value="ENSGAGP00000007804.1"/>
        <property type="gene ID" value="ENSGAGG00000006216.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04127">
        <property type="entry name" value="Rab27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000402">
        <property type="entry name" value="Ras-related protein Rab-27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041837">
        <property type="entry name" value="Rab27a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="218" mass="24574" checksum="7E62CAA1ED11512E" modified="2019-05-08" version="1">MTDGDYDYLIKLLALGDSGVGKTTFLYRYTDNKFNPKFITTVGIDFREKRVIYHSRGPNGSPGKAFKVHLQLWDTAGQERFRSLTTAFFRDAMGFLLMFDLTSQQSFLNVRNWMSQLQANAYCENPDIVLLGNKADLSDQREVNERQARDLADKYGIPYFETSAATGQNVDKAVEMLLDLIMKRMEQCVDKTQVSDTANGGSSGKLDSAKPEEKKCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1C1</accession>
    <name>A0A452H1C1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H1C1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008179"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009419.1">
        <property type="protein sequence ID" value="ENSGAGP00000008179.1"/>
        <property type="gene ID" value="ENSGAGG00000006496.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF188">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="250"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="658" mass="72472" checksum="7BF229441EFDC2A4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H230</accession>
    <name>A0A452H230_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NR LBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H230"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008619"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009911.1">
        <property type="protein sequence ID" value="ENSGAGP00000008619.1"/>
        <property type="gene ID" value="ENSGAGG00000006809.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06948">
        <property type="entry name" value="NR_LBD_COUP-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000003">
        <property type="entry name" value="Coup transcription factor 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01282">
        <property type="entry name" value="COUPTNFACTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NR LBD" evidence="5">
        <location>
            <begin position="56"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="292" mass="32607" checksum="75563338B9FF4AF0" modified="2019-05-08" version="1">MRDAPPAHLTPSTELQGCGELRQRTPAVQRGRMAHTQTSPGQYLLNNGDPYNGHSYLTGFISLLLRAEPYPTSRYGAQCLQSNIMGIENICELAARLLFSAIEWAKNIPFFPDFQLSDQVSLLRMTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRVFQEQVEKLKALHVDSAEYSCLKAIALFTPDAVGLSDLGPVESIQEKSQCALEEYVRNQYPNQPSRFGRLLLRMPSLRIVSAPVIEQLFFVRLVGKTPIETLIRDMLLSGSSFNWPYIPMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H307</accession>
    <name>A0A452H307_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H307"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008988"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010322.1">
        <property type="protein sequence ID" value="ENSGAGP00000008988.1"/>
        <property type="gene ID" value="ENSGAGG00000007100.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000522">
        <property type="entry name" value="zinc finger protein 285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000761">
        <property type="entry name" value="Zinc finger protein 449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000238">
        <property type="entry name" value="Zinc finger protein 485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000038">
        <property type="entry name" value="Zinc finger protein 624"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001035">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050826">
        <property type="entry name" value="Krueppel_C2H2_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24377">
        <property type="entry name" value="IP01015P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="273"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="472"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="519"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="33"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="440"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="459"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="575" mass="63820" checksum="07578C4A2CA52D07" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3U0</accession>
    <name>A0A452H3U0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Galactose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="11">2.7.7.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:13989"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.7.7.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.12" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A452H3U0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009279"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010650.1">
        <property type="protein sequence ID" value="ENSGAGP00000009279.1"/>
        <property type="gene ID" value="ENSGAGG00000007327.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000001">
        <property type="entry name" value="Galactose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000028">
        <property type="entry name" value="Galactose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001937">
        <property type="entry name" value="GalP_UDPtransf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019779">
        <property type="entry name" value="GalP_UDPtransf1_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005850">
        <property type="entry name" value="GalP_Utransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00209">
        <property type="entry name" value="galT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943:SF1">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02744">
        <property type="entry name" value="GalP_UDP_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00117">
        <property type="entry name" value="GAL_P_UDP_TRANSF_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 11" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase C-terminal" evidence="13">
        <location>
            <begin position="75"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="264" mass="30551" checksum="F7302E969DDCC76A" modified="2019-05-08" version="1">MCFHPWSDLTLPLMSLAEIRAVIDKWAELAVELGSSYPWVQIFENKGAMMGCSNPHPHCQVWASNFLPNEAQLEDRTQRQHLQERGVPLLLEYAQLEAQRKERLVVENADWLVVVPYWAVWPFQTLLLPRRQVCRLQELSDSERDSLASIMKRLLTKYDNLFEVSFPYSMGWHGAPTGPRLGADCGHWQLHAHYYPPLLRSAAVRKFMVGYEMLAQAQRDLTPEQSSKSNWTYYRYLHSPRRPKGGAHRGGSWSFLKGQAEILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3U8</accession>
    <name>A0A452H3U8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3U8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009122"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010470.1">
        <property type="protein sequence ID" value="ENSGAGP00000009122.1"/>
        <property type="gene ID" value="ENSGAGG00000007202.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17132">
        <property type="entry name" value="Ubl_TMUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040352">
        <property type="entry name" value="TMUB1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14557">
        <property type="entry name" value="PROTEIN C7ORF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14557:SF4">
        <property type="entry name" value="TRANSMEMBRANE AND UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="164"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="68"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="308" mass="32972" checksum="A85F8CC1AE3D995B" modified="2019-05-08" version="1">MKPPGMTIIEGVGDEVTVVTGVVVLVLALVLAWLSTYVADGNNQLLGTIVTTGESSVIHLSHVERYVGNSVTSEPTEPQGSADSAEEKAEEEGGAASNLGPAVEQGGNGSTSESSLDHLLNIQGLPKRTSSSESGALEQPGQESQGTSQMSHIREESEPGSGLIKVRLKFLNDTEEVAMVRPEDTVGVLKSKYFPGQENQMKFIYQGQLLQDQARTLRSLNIMDNCVIHCHLSQTASSPLPDSGVTSAEAAGVTVNIGNLMLPVFMVMLAVIWYCRINYRQFFTAPATVSLVGVTVFFSFLVFGMYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H425</accession>
    <name>A0A452H425_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16 17">Ubiquitin-protein ligase E3A</fullName>
            <ecNumber evidence="5 17">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 17">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 17">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="5 17"/>
    <dbReference type="AlphaFoldDB" id="A0A452H425"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009261"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010629.1">
        <property type="protein sequence ID" value="ENSGAGP00000009261.1"/>
        <property type="gene ID" value="ENSGAGG00000007313.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030518">
        <property type="term" value="P:nuclear receptor-mediated steroid hormone signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042752">
        <property type="term" value="P:regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000026">
        <property type="entry name" value="E3 ubiquitin-protein ligase HACE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000004">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000003">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000008">
        <property type="entry name" value="Putative ubiquitin-protein ligase E3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.130.10">
        <property type="entry name" value="Ubiquitin-protein ligase E3A, N-terminal zinc-binding domain (AZUL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032353">
        <property type="entry name" value="AZUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042556">
        <property type="entry name" value="AZUL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017134">
        <property type="entry name" value="UBE3A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF10">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16558">
        <property type="entry name" value="AZUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037201">
        <property type="entry name" value="Ubiquitin-protein_ligase_E6-AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="6 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 17" id="KW-0647">Proteasome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 17 18" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HECT" evidence="20">
        <location>
            <begin position="552"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="89"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="176"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="176"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR037201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="841" mass="96505" checksum="738954B2C29DF7DE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H491</accession>
    <name>A0A452H491_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the POU transcription factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H491"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009440"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010847.1">
        <property type="protein sequence ID" value="ENSGAGP00000009440.1"/>
        <property type="gene ID" value="ENSGAGG00000007470.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000056">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000007">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF44">
        <property type="entry name" value="BRAIN-SPECIFIC HOMEOBOX_POU DOMAIN PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="11">
        <location>
            <begin position="181"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="274"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="276"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="340" mass="37720" checksum="B834FAA6086A5473" modified="2019-05-08" version="1">MMSMNSKQAFSMHPILHEPKYTHLHTSSEAIRRACLPAPQIQGNIFAGFDETLLRGAEALAAVDIVSQKTHPFKPDATYHTMSSVSCTPTSSSVHLHHPSVLTTHHHHHHHQPSQGLEGELLDHLNSAIPLGGVPGPEVGSTPSHPHSHMPALNHMAHHPQSMNMSHPHGLASHAVISGPETETDPRELESFAERFKQRRIKLGVTQADVGSALANLKIPGVGCLSQSTICRFESLTLSHNNMVALKPILEAWLEEAERAQREKMTKPEIYTGGDKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSATY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H5E6</accession>
    <name>A0A452H5E6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H5E6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009863"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011330.1">
        <property type="protein sequence ID" value="ENSGAGP00000009863.1"/>
        <property type="gene ID" value="ENSGAGG00000007764.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="125" mass="14342" checksum="C5EB161610193A84" modified="2019-05-08" version="1">MLFSVPSRMVLALDKWFWWHEYWFPPGFSWEDIKESGSISYPKPRDLLHIVPYALLLVGVRYLFQRINSLEKSGAISMESLQRAVRRSVNASSAASDNLTRSGPRAFTRSVKGLALVAKWGINRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H689</accession>
    <name>A0A452H689_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FERM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000010210"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011718.1">
        <property type="protein sequence ID" value="ENSGAGP00000010210.1"/>
        <property type="gene ID" value="ENSGAGG00000008001.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13184">
        <property type="entry name" value="FERM_C_4_1_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17201">
        <property type="entry name" value="FERM_F1_EPB41L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000001">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000001">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000002">
        <property type="entry name" value="Erythrocyte protein band 4.1-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008379">
        <property type="entry name" value="Band_4.1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007477">
        <property type="entry name" value="SAB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF24">
        <property type="entry name" value="BAND 4.1-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05902">
        <property type="entry name" value="4_1_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04382">
        <property type="entry name" value="SAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="7">
        <location>
            <begin position="101"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="431"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="654"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1464"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1541"/>
            <end position="1660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1698"/>
            <end position="1755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1811"/>
            <end position="1839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="447"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="550"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="586"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="679"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="709"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="821"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="902"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="933"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="943"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1003"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1022"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1143"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1303"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1436"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1475"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1541"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1624"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1700"/>
            <end position="1713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1739"/>
            <end position="1752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1936" mass="210434" checksum="AF557736B47E0C27" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H824</accession>
    <name>A0A452H824_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Receptor-type tyrosine-protein phosphatase S</fullName>
            <ecNumber evidence="7">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="36 37">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="37" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Postsynaptic density</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="26">Synapse</location>
            <location evidence="26">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010908"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012501.1">
        <property type="protein sequence ID" value="ENSGAGP00000010908.1"/>
        <property type="gene ID" value="ENSGAGG00000008474.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd05738">
        <property type="entry name" value="IgI_2_RPTP_IIa_LAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05739">
        <property type="entry name" value="IgI_3_RPTP_IIa_LAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14624">
        <property type="entry name" value="R-PTPc-D-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000010">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000027">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000036">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000066">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000068">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000144">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000015">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000082">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000128">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000002">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000001">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase F isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000098">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase F isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045905">
        <property type="entry name" value="R-PTP-delta_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF11">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23" id="KW-0966">Cell projection</keyword>
    <keyword evidence="25" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="22" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="24" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="19 31" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="21" id="KW-0675">Receptor</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0770">Synapse</keyword>
    <keyword evidence="9" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="11 31" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 31" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="1138"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="34">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="34">
        <location>
            <begin position="107"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="196"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="291"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="389"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="483"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="585"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="694"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="792"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="35">
        <location>
            <begin position="891"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="32">
        <location>
            <begin position="1272"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="33">
        <location>
            <begin position="1447"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="32">
        <location>
            <begin position="1559"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="33">
        <location>
            <begin position="1736"/>
            <end position="1809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1169"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="1185"/>
            <end position="1194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00073611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1826" mass="204608" checksum="336FAA84E887F1E8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8X5</accession>
    <name>A0A452H8X5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H8X5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011249"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012884.1">
        <property type="protein sequence ID" value="ENSGAGP00000011249.1"/>
        <property type="gene ID" value="ENSGAGG00000008699.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF142">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="32"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="76" mass="9007" checksum="0097314D24A7EF7A" modified="2019-05-08" version="1">MFIKKNKKSQLPARVQFRQVLSGGPMSFQMPVMFEEVAVYFSEDEWALLDEKQKELYRDVMQENYETLLSLGEQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H903</accession>
    <name>A0A452H903_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H903"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012859.1">
        <property type="protein sequence ID" value="ENSGAGP00000011227.1"/>
        <property type="gene ID" value="ENSGAGG00000008684.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041404">
        <property type="entry name" value="DUF5588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31919">
        <property type="entry name" value="ZINC FINGERS AND HOMEOBOXES PROTEIN 1, ISOFORM 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31919:SF1">
        <property type="entry name" value="ZINC FINGERS AND HOMEOBOXES PROTEIN 1, ISOFORM 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17826">
        <property type="entry name" value="DUF5588"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="400" mass="44945" checksum="60D4DDA262EB13BB" modified="2019-05-08" version="1">MVPVGEPAGRAPHRLLARRCGGRSSPGFSRSRPGGALAGDSVAREAMELALGFEFEESVFAQSRDRGRGGGCAAYTAKRCEPQWFCEDANSEGDVEILTAKKFRGDLAYRQQEFQKALYEYSSCLLLLPSSNIAMRRDVQEGQARFAIYCRLKNIEKKITCLQQLISLHPFNPWNWKLLAETYMSFLQTLPPSFISEMKHLQCKDCTANNHVFQTSPGLFGKEMNLQCHKTLSGRQDVWSTLSTKTTRDNSVSYSDSHMVEGSLHITEECETRDKMKHATFESWGQEALKDVWIKACASFVRTRLLLQITQLQQSSFALAQNLKVQQETEDKVKGFGLKEESLLLITEVMGEDLIPEKLKEDGQGEIKCVGPSALTSLVTTSAIEFEGKWFKKLQDNLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H921</accession>
    <name>A0A452H921_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H921"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011299"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012940.1">
        <property type="protein sequence ID" value="ENSGAGP00000011299.1"/>
        <property type="gene ID" value="ENSGAGG00000008740.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048787">
        <property type="term" value="C:presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035612">
        <property type="term" value="F:AP-2 adaptor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08374">
        <property type="entry name" value="C2F_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037725">
        <property type="entry name" value="C2F_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037721">
        <property type="entry name" value="Ferlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546">
        <property type="entry name" value="FER-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546:SF32">
        <property type="entry name" value="OTOFERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="91"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="223" mass="25457" checksum="36DB0E08FBD8C436" modified="2019-05-08" version="1">MLCAPAFSCPGLAAGSTARPARLSARSAHTGQKKQTDEHLALAVLRQWEEIPRVGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKSYELWVIIWNTNEVQLEDNAFMTGEKMSDIYVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLMAEEKIVISKKASMFSWDETEYKIPARLTLQVWDADHFSADDFLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HA42</accession>
    <name>A0A452HA42_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the small GTPase superfamily. RasD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HA42"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011586"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013265.1">
        <property type="protein sequence ID" value="ENSGAGP00000011586.1"/>
        <property type="gene ID" value="ENSGAGG00000008945.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04143">
        <property type="entry name" value="Rhes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000475">
        <property type="entry name" value="GTP-binding protein Rhes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052236">
        <property type="entry name" value="Small_GTPase_RasD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149:SF4">
        <property type="entry name" value="DEXAMETHASONE-INDUCED RAS-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149">
        <property type="entry name" value="MIP08469P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="278" mass="31474" checksum="6BF5EF0F5E4FCA38" modified="2019-05-08" version="1">MRPAAMIKKLCQSESELSIPAKNCYRMVVLGSSKVGKTAIVSRFLTGRFEEQYTPTIEDFHRKFYSIRGEVYQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRDSFEEVQRLKLQILETKSCLKNKTKENMEVPLVICGNKGDRDFYREVEPREIEQLVGADPQKCAYFEISAKKNSSLDQMFQALFAMAKLPSEMSPDLHRKVSVQYCDMLHKKALKGKKLLKEGAEGSGGEAYGIVAPFARRPSVHSDLMYIREKAIGGSQAKDKERCVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAN6</accession>
    <name>A0A452HAN6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HAN6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011806"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013521.1">
        <property type="protein sequence ID" value="ENSGAGP00000011806.1"/>
        <property type="gene ID" value="ENSGAGG00000009101.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030055">
        <property type="term" value="C:cell-substrate junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004614">
        <property type="term" value="F:phosphoglucomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014706">
        <property type="term" value="P:striated muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000002">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000004">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000005">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000007">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045244">
        <property type="entry name" value="PGM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005737">
        <property type="entry name" value="PRK07564.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22573:SF27">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22573">
        <property type="entry name" value="PHOSPHOHEXOMUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24947">
        <property type="entry name" value="PGM1_C_vert_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="5">
        <location>
            <begin position="23"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="6">
        <location>
            <begin position="200"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="7">
        <location>
            <begin position="313"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="567" mass="62569" checksum="618C0DCE22C63ADB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEE6</accession>
    <name>A0A452HEE6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM163 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HEE6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013177"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015085.1">
        <property type="protein sequence ID" value="ENSGAGP00000013177.1"/>
        <property type="gene ID" value="ENSGAGG00000010097.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000015088.1">
        <property type="protein sequence ID" value="ENSGAGP00000013180.1"/>
        <property type="gene ID" value="ENSGAGG00000010097.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029379">
        <property type="entry name" value="FAM163"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040280">
        <property type="entry name" value="FAM163B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31396:SF2">
        <property type="entry name" value="PROTEIN FAM163B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31396">
        <property type="entry name" value="PROTEIN FAM163B MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15069">
        <property type="entry name" value="FAM163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="161" mass="17939" checksum="BA705ECDB66B8BF8" modified="2019-05-08" version="1">MTAGTVVITGGILATVILLCIIAVLCYCRLQYYCCKKEESEEDEEEPDFAVHSHIPPLHSNRNVVLTNGPSLYTSSSSPFSNQHSQCRTVYPACTHYEPPTFFLQQPDEIHNGGDRVSYKTISQEDIELPVNVTSLQALNPNRLSAMREAFSRSRSISTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEW9</accession>
    <name>A0A452HEW9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HEW9"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015303.1">
        <property type="protein sequence ID" value="ENSGAGP00000013370.1"/>
        <property type="gene ID" value="ENSGAGG00000010232.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="55" mass="6453" checksum="CD238B59893AB641" modified="2019-05-08" version="1">WDRGGAARNYSSRRLGLAEKKSFPAFSGPKDFSIWNEFVQRKKVFFLTSRRSYCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HI34</accession>
    <name>A0A452HI34_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHHC U11-48K-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HI34"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014564"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016659.1">
        <property type="protein sequence ID" value="ENSGAGP00000014564.1"/>
        <property type="gene ID" value="ENSGAGG00000011062.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051591">
        <property type="entry name" value="UPF0224_FAM112_RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21402:SF5">
        <property type="entry name" value="GAMETOCYTE SPECIFIC FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21402">
        <property type="entry name" value="GAMETOCYTE SPECIFIC FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="4">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="CHHC U11-48K-type" evidence="4">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="191" mass="22028" checksum="B3D1E4A2B8973A50" modified="2019-05-08" version="1">LEESSTDALDPEKLLQCPYDRYHQIRACRFPYHLIKCRKNHPDIAEQLATCPFNARHQVPREELRHHISSCDDKRCIEPDIVSQSNNCHREMMNTVSMWQPPPCDEDWDKGEEVDDTRLLSDNSYLISFSPGSSVVMEPKNNLMPGMRAPKSLPYDLSWKSSNHATFSLSYILNSGDWERGVFCVADANLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIV6</accession>
    <name>A0A452HIV6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Discoidin domain-containing receptor 2</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">CD167 antigen-like family member B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Neurotrophic tyrosine kinase, receptor-related 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Receptor protein-tyrosine kinase TKT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Tyrosine-protein kinase TYRO10</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">Binds hydroxyproline-rich sequence motifs in fibrillar, glycosylated collagen, such as the GQOGVMGFO motif, where O stands for hydroxyproline. Interacts with SRC. Interacts (tyrosine phosphorylated) with SHC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452HIV6"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016948.1">
        <property type="protein sequence ID" value="ENSGAGP00000014833.1"/>
        <property type="gene ID" value="ENSGAGG00000011230.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038062">
        <property type="term" value="F:protein tyrosine kinase collagen receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010715">
        <property type="term" value="P:regulation of extracellular matrix disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05097">
        <property type="entry name" value="PTKc_DDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000007">
        <property type="entry name" value="Discoidin domain receptor tyrosine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1190:FF:000001">
        <property type="entry name" value="Discoidin domain receptor tyrosine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000053">
        <property type="entry name" value="Epithelial discoidin domain-containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000082">
        <property type="entry name" value="Epithelial discoidin domain-containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048525">
        <property type="entry name" value="DDR1-2_DS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002011">
        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF297">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21114">
        <property type="entry name" value="DDR1-2_DS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00239">
        <property type="entry name" value="RECEPTOR_TYR_KIN_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 28" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 27" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="28">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019483624" description="Discoidin domain-containing receptor 2" evidence="28">
        <location>
            <begin position="18"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="30">
        <location>
            <begin position="24"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="29">
        <location>
            <begin position="579"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="509"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="522"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00065042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00070183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00076913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00078393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00079441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00079636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="873" mass="99436" checksum="28F8BFCA346F0B56" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HM06</accession>
    <name>A0A452HM06_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HM06"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015971"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018250.1">
        <property type="protein sequence ID" value="ENSGAGP00000015971.1"/>
        <property type="gene ID" value="ENSGAGG00000012017.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007099">
        <property type="term" value="P:centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045931">
        <property type="term" value="P:positive regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042481">
        <property type="entry name" value="CCDC57"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46725">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46725:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="606"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="849"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="137"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="378"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="709"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="861"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="989" mass="114500" checksum="5921033A0A8E8BD5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMW1</accession>
    <name>A0A452HMW1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HMW1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016292"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018598.1">
        <property type="protein sequence ID" value="ENSGAGP00000016292.1"/>
        <property type="gene ID" value="ENSGAGG00000012216.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026642">
        <property type="entry name" value="Glcci1/FAM117"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14972">
        <property type="entry name" value="AGAP011572-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14972:SF3">
        <property type="entry name" value="GLUCOCORTICOID-INDUCED TRANSCRIPT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15388">
        <property type="entry name" value="FAM117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="211"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="110"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="240"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="370" mass="39310" checksum="734D12535A52E811" modified="2019-05-08" version="1">PSTNISSSSSSTPSLTPPGHHPPQRMRRGAAGSPTAANGAAGNGAGGGAASSAGTSRLLQPIRATVPYQLLRGSQHSPTRSPSASVGSNTGPAGGGGGRGSSPPSPTPPVAVAAPPGSGVADPGRVKGRQRRSPESGGSRRSSSPERRSPSSPVYRVDRPKSQQVRTSGTIRRTSSLDTITGPYLTGQWPRDPHVHYPSCMKDKATQTPSCWAEEGSEKRSHQRSASWGSADQLKEQIAKLRQQLQRSKQSSRHSKEKERQSPLHGNHIAISQTQASISRSVPMPLSNISVPKSTVSRVPCNVEGISPELEKVFIKETSGKEEVSKVKFKFLGCFYIFYGQNLQRNSHRSSMPPIGWGLRLWAEAAPRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HN50</accession>
    <name>A0A452HN50_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HN50"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016403"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018707.1">
        <property type="protein sequence ID" value="ENSGAGP00000016392.1"/>
        <property type="gene ID" value="ENSGAGG00000012278.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000018711.1">
        <property type="protein sequence ID" value="ENSGAGP00000016396.1"/>
        <property type="gene ID" value="ENSGAGG00000012278.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000018716.1">
        <property type="protein sequence ID" value="ENSGAGP00000016401.1"/>
        <property type="gene ID" value="ENSGAGG00000012278.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000018718.1">
        <property type="protein sequence ID" value="ENSGAGP00000016403.1"/>
        <property type="gene ID" value="ENSGAGG00000012278.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20111">
        <property type="entry name" value="MBT_SFMBT1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20113">
        <property type="entry name" value="MBT_SFMBT1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20115">
        <property type="entry name" value="MBT_SFMBT1_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20117">
        <property type="entry name" value="MBT_SFMBT1_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09581">
        <property type="entry name" value="SAM_Scm-like-4MBT1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000027">
        <property type="entry name" value="scm-like with four MBT domains protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.190">
        <property type="entry name" value="SLED domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004092">
        <property type="entry name" value="Mbt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047352">
        <property type="entry name" value="MBT_SFMBT1_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047351">
        <property type="entry name" value="MBT_SFMBT1_rpt3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050548">
        <property type="entry name" value="PcG_chromatin_remod_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037604">
        <property type="entry name" value="Scm-like-4MBT1/2_SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021987">
        <property type="entry name" value="SLED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038348">
        <property type="entry name" value="SLED_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF77">
        <property type="entry name" value="SCM-LIKE WITH FOUR MBT DOMAINS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02820">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12140">
        <property type="entry name" value="SLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00561">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51079">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <feature type="repeat" description="MBT" evidence="5">
        <location>
            <begin position="20"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="MBT" evidence="5">
        <location>
            <begin position="128"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="MBT" evidence="5">
        <location>
            <begin position="242"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="MBT" evidence="5">
        <location>
            <begin position="357"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="7">
        <location>
            <begin position="794"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="642"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="664"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="700"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="867" mass="97263" checksum="9E6683B858F058E6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNT0</accession>
    <name>A0A452HNT0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HNT0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016626"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018979.1">
        <property type="protein sequence ID" value="ENSGAGP00000016626.1"/>
        <property type="gene ID" value="ENSGAGG00000012434.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000823">
        <property type="entry name" value="replication initiator 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000870">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000200">
        <property type="entry name" value="zinc finger protein 510 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002604">
        <property type="entry name" value="Zinc finger protein 715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF54">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF26.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="15">
        <location>
            <begin position="44"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="271"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="327"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="14">
        <location>
            <begin position="351"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="355"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="381"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="409"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="437"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="94"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="103"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="464" mass="52463" checksum="F62C6DCF61AC9C28" modified="2019-05-08" version="1">MGAAGSCGLHIPCPRCFLPPPLAWDGEPRPEGRQGRKMAAAEPVTFEEVAVYFSEEEWALLDLGQRALYRDVMQENYETVTSLGTDSCLDLLSVPAGDGMMSENKEENPQQEGSEQREPQGTVSGRAEGHVSQSPEQREACEIQRRPKRKQGNHSEETLGKSTHKSRDIKKIKETVQLRIPTGESPYSCGDCGKSLSQSLTLIHRQRIHMGEKPYKCNECGKSFSVSSKFLLHQRTHTGERPYDCPVCGKSFSVSSNLIQHQRIHTGEKPYKCPDCGKSFNQSSNLLSHQRIHTGEKPFECLECRKSFSRRSTLLSHQRIHTEEKPYTCAECGKSFGHSAHLTSHQRTHTGEKPYKCSECGKSFSQTLRKHQRIHSGERPYKCLHCGKSFCSSSALCNHQSTHLEDRPYKCNECRKSFSQSSNLIQHQRIHTGERPYNCPDCGKSFSRKSTLIKHQRTHVAERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HR96</accession>
    <name>A0A452HR96_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BEN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HR96"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017591"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020067.1">
        <property type="protein sequence ID" value="ENSGAGP00000017591.1"/>
        <property type="gene ID" value="ENSGAGG00000013065.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2590">
        <property type="entry name" value="BEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018379">
        <property type="entry name" value="BEN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053072">
        <property type="entry name" value="BEN_domain_protein_7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35068">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35068:SF1">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10523">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01025">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51457">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BEN" evidence="2">
        <location>
            <begin position="225"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="369" mass="40954" checksum="863E24BFAAC0010D" modified="2019-05-08" version="1">MRRLLNDSTGRIYQRVGKEGEKLKEEPQDLDLAWAQRLTALADSQASLLPSQNGAWNEVSSQASHFSGQYGTRSKSFQNETRTVGSNGEHPGISSSVGPNCCTCNCQSTLQAILQELKIMRKLMQIQAVGTQNRQQPPIPLICAQKSTISRKRNKKKKLPQKTLEPITVNKKPSTVEKEKKLSANSERSSLQAAEHTLKPETHVSGFGIILESSSSDPEVQLAEGFDVFMPKSQLDSILSNYTRSGSLLFRKLVCAFFDDKTLANSLPNGKRKRGLNDNRKGLDQNIVGAIKVFTEKYCTANHVDKLPGPRDWVQILQDQIKLARRRLKRGSAETTDCDEKLDISLLQTGSILEIATDKLVDSKPDFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUU8</accession>
    <name>A0A452HUU8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="21">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="function">
        <text evidence="19">RNA-dependent RNA polymerase that catalyzes the extension of a non-coding RNA (ncRNA) at the 3'-end using the four ribonucleoside triphosphates as substrates. An internal ncRNA sequence near the 3'-end serves as a template in a single-round Pol II-mediated RNA polymerization reaction. May decrease the stability of ncRNAs that repress Pol II-mediated gene transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:21249"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:21249"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:21250"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 3'-end ribonucleotidyl-ribonucleotide-RNA + H2O = a 3'-end ribonucleotide-RNA + a ribonucleoside 5'-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:77763"/>
            <dbReference type="Rhea" id="RHEA-COMP:17428"/>
            <dbReference type="Rhea" id="RHEA-COMP:18982"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:197502"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:77764"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="21"/>
    <dbReference type="AlphaFoldDB" id="A0A452HUU8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018852"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021490.1">
        <property type="protein sequence ID" value="ENSGAGP00000018852.1"/>
        <property type="gene ID" value="ENSGAGG00000013947.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000026">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1070.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000043">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 21" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="8 21" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 21" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="25">
        <location>
            <begin position="42"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="24">
        <location>
            <begin position="205"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="26">
        <location>
            <begin position="472"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="27">
        <location>
            <begin position="571"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="28">
        <location>
            <begin position="657"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="22">
        <location>
            <begin position="711"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="23">
        <location>
            <begin position="1086"/>
            <end position="1176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00058652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1178" mass="134350" checksum="67195A9B1005A689" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HV00</accession>
    <name>A0A452HV00_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leiomodin-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Myofibril</location>
            <location evidence="6">Sarcomere</location>
            <location evidence="6">M line</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tropomodulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HV00"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018970"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021627.1">
        <property type="protein sequence ID" value="ENSGAGP00000018970.1"/>
        <property type="gene ID" value="ENSGAGG00000014038.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005865">
        <property type="term" value="C:striated muscle thin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051694">
        <property type="term" value="P:pointed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000078">
        <property type="entry name" value="Leiomodin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004934">
        <property type="entry name" value="TMOD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901:SF3">
        <property type="entry name" value="LEIOMODIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901">
        <property type="entry name" value="TROPOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03250">
        <property type="entry name" value="Tropomodulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="104"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="466"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="408"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="161"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="484"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="580" mass="66807" checksum="3B41F591DDF26892" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWS1</accession>
    <name>A0A452HWS1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="27">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22 27">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="27">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="27"/>
    <dbReference type="AlphaFoldDB" id="A0A452HWS1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019636"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022364.1">
        <property type="protein sequence ID" value="ENSGAGP00000019636.1"/>
        <property type="gene ID" value="ENSGAGG00000014485.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01238">
        <property type="entry name" value="PH_Btk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05114">
        <property type="entry name" value="PTKc_Tec_Rlk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10396">
        <property type="entry name" value="SH2_Tec_Itk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11905">
        <property type="entry name" value="SH3_Tec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000052">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000210">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000151">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000041">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035572">
        <property type="entry name" value="Tec_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001562">
        <property type="entry name" value="Znf_Btk_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00779">
        <property type="entry name" value="BTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00402">
        <property type="entry name" value="TECBTKDOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00107">
        <property type="entry name" value="BTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51113">
        <property type="entry name" value="ZF_BTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="14 26 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="21" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-0391">Immunity</keyword>
    <keyword evidence="12 27" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 26 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16 23" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="4 24" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8 27" id="KW-0808">Transferase</keyword>
    <keyword evidence="20 27" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 25" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="31">
        <location>
            <begin position="4"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="30">
        <location>
            <begin position="181"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="29">
        <location>
            <begin position="249"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="32">
        <location>
            <begin position="372"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="631" mass="73635" checksum="A0448BC756797CA7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZ23</accession>
    <name>A0A452HZ23_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PHD finger protein 13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Modulates chromatin structure and DNA damage response by regulating key determinants of chromatin compaction and DNA damage response. Binds H3K4me3-containing chromatin and promotes DNA condensation by recruiting corepressors such as TRIM28 and H3K9 methyltransferase SETDB1. Required for normal chromosome condensation during the early stages of mitosis. Required for normal chromosome separation during mitosis. Increases both chromatin-associated levels and activity of H3K9 methyltransferases, such as SETDB1, thus enhancing H3K9 trimethylation. Essential for testicular stem-cell differentiation and sustained spermatogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZ23"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020419"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023267.1">
        <property type="protein sequence ID" value="ENSGAGP00000020419.1"/>
        <property type="gene ID" value="ENSGAGG00000015050.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15632">
        <property type="entry name" value="PHD_PHF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000039">
        <property type="entry name" value="PHD finger protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041947">
        <property type="entry name" value="PHD_PHF13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14571">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SET-26-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14571:SF13">
        <property type="entry name" value="PHD FINGER PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="14">
        <location>
            <begin position="330"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="396" mass="44694" checksum="485D747650DC5055" modified="2019-05-08" version="1">MLGAVVLSRTTIPSSPRAEPRRSRSRGGRTTAPSSPFSSHGARPVPRLPPTGTGRFEVSALPGEIELILRYRARAPGSTWSRLPDPGRLRWDMDPDPCASPFPPEDFSPSSKRHRTVEDFNKFCTFVLAYAGYIPYPQEETPLRSSPSPSNSTGGTIDSDSWDPRFNDLPSAVSLPVKSRKSQLKRAKPYVSLFQRAKPSSFLSERKQTDKIRKKKKMKRRETEAPMEEEYGEKLLKLEAEDSYVETPMSPSSEGDPQSVTDHCSVWDSDTPSNVSYPPETAEQGMEIQSVGKRTVIRQGKQVVFRDEDGTGDDEDIMVDSDDDSWDLVTCFCMKPFAGRPMIECNECHTWIHLSCAKIRKSNVPEVYVCQKCRDSKFDIRRSNRSRTGSRRRFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZ65</accession>
    <name>A0A452HZ65_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Synaptojanin-2-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mitochondrial outer membrane protein 25</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Binds (via the PDZ domain) to isoform 2A of SYNJ2 (via the unique motif in the C-terminus). Interacts (via C-terminus) with RALBP1. Interacts (via PDZ domain) with ACVR2A (via C-terminus) and ACVR2B (via C-terminus). Forms a ternary complex with ACVR2A and RALBP1. Interacts with MAPK12. Interacts with DLL1; enhances DLL1 protein stability, and promotes notch signaling in endothelial cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZ65"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020515"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023368.1">
        <property type="protein sequence ID" value="ENSGAGP00000020515.1"/>
        <property type="gene ID" value="ENSGAGG00000015102.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097120">
        <property type="term" value="P:receptor localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06709">
        <property type="entry name" value="PDZ_SYNJ2BP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000161">
        <property type="entry name" value="Synaptojanin-2-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF51">
        <property type="entry name" value="DISKS LARGE 1 TUMOR SUPPRESSOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="13"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00063547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="125" mass="13336" checksum="C49606E0F5FBE81B" modified="2019-05-08" version="1">MNGGVGWVLSEEVIDLTRGPSGLGFNIVGGMDQQYIANDSGIYVSRIKGDGAAALDGRLQEGDKILAINGRDLKNLLHKDAVELFRNAGCDVSLKVQHRVGIASSWQLSLRPCYHGLGQTVGDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZL9</accession>
    <name>A0A452HZL9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1-aminocyclopropane-1-carboxylate synthase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Does not catalyze the synthesis of 1-aminocyclopropane-1-carboxylate but is capable of catalyzing the deamination of L-vinylglycine.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZL9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020670"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023545.1">
        <property type="protein sequence ID" value="ENSGAGP00000020670.1"/>
        <property type="gene ID" value="ENSGAGG00000015226.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000102">
        <property type="entry name" value="1-aminocyclopropane-1-carboxylate synthase homolog (inactive)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000032">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF17">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795">
        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="6">
        <location>
            <begin position="12"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="5">
        <location>
            <begin position="419"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="209"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00057291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00068556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="888" mass="101757" checksum="9C9C0DC2A6C9B7C7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZQ8</accession>
    <name>A0A452HZQ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alpha-1-syntrophin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the syntrophin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZQ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020671"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023546.1">
        <property type="protein sequence ID" value="ENSGAGP00000020671.1"/>
        <property type="gene ID" value="ENSGAGG00000015083.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000023547.1">
        <property type="protein sequence ID" value="ENSGAGP00000020672.1"/>
        <property type="gene ID" value="ENSGAGG00000015083.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
        <property type="term" value="C:dystrophin-associated glycoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06801">
        <property type="entry name" value="PDZ_syntrophin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01258">
        <property type="entry name" value="PHsplit_syntrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000329">
        <property type="entry name" value="alpha-1-syntrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000052">
        <property type="entry name" value="Syntrophin beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041428">
        <property type="entry name" value="PHsplit_syntrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015482">
        <property type="entry name" value="Syntrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055108">
        <property type="entry name" value="Syntrophin_4th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10554:SF6">
        <property type="entry name" value="ALPHA-1-SYNTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10554">
        <property type="entry name" value="SYNTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18012">
        <property type="entry name" value="PH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23012">
        <property type="entry name" value="Syntrophin_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="17">
        <location>
            <begin position="73"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="16">
        <location>
            <begin position="278"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="490" mass="53413" checksum="CCC0A245208FCE59" modified="2019-05-08" version="1">MAAARRAPKSGLLELRAPGEQWLRVLVTLAEDFLTVSPGKGAGAEEPPPAPAQLNGGEPCAQVPESLTNVKRTVRIVKQDVGGLGISIKGGRENKMPILISKIFKGLAADQTEALYVGDAILSVNGADLSEATHDEAVQALKKTGKEVVLEVKYMKEISPYFKNSSSGATVSWDPSPAVSLQKQASPILSLRDLKEGKNMPLKMCYVSRKCLPNDPENRYLEVCSADGRIPLFLRAKDEVTAQSWFNTIHTNVNALVPRVKEELRALLAAAGVAGSKEIKHIGWLTEQLPGDGTKNILTILTEKDLLFYSSLPQTRDALNKPTHSYPLIATRLVHSGPSKGSHLYDSELSFALRTGTKKGVETHLFSVETHRDLAMWTRMLVDGCHSAAEFTQEVSAACTWNGRDCTLSIHIDKGFTIFTTEPGISRTILLQQPFEKLQMSSDDGAKMLYLDFGSAEGEIQLDLHSCPKTIVFIIHSFLSAKVTRLGLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0Y1</accession>
    <name>A0A452I0Y1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I0Y1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021112"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024049.1">
        <property type="protein sequence ID" value="ENSGAGP00000021112.1"/>
        <property type="gene ID" value="ENSGAGG00000015507.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000002">
        <property type="entry name" value="Scavenger receptor cysteine-rich type 1 protein M130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF487">
        <property type="entry name" value="SOLUBLE SCAVENGER RECEPTOR CYSTEINE-RICH DOMAIN-CONTAINING PROTEIN SSC5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="5">
        <location>
            <begin position="22"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="111" mass="11953" checksum="125DDEA978C6D3AE" modified="2019-05-08" version="1">VTPSCLFPWVFADAGISEVAQLRLVNSKSHCAGRIEVLHNQQWGTVCDDSWDLQDAGVVCRQLGCGTALSAPRGARFGQGSDHIWMDNMNCTGTEVALSDCRARIELGCGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1L3</accession>
    <name>A0A452I1L3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">Beta-galactosidase</fullName>
            <ecNumber evidence="5 16">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. May form higher multimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="5 16"/>
    <dbReference type="AlphaFoldDB" id="A0A452I1L3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021387"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024359.1">
        <property type="protein sequence ID" value="ENSGAGP00000021387.1"/>
        <property type="gene ID" value="ENSGAGG00000015700.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006689">
        <property type="term" value="P:ganglioside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042340">
        <property type="term" value="P:keratan sulfate proteoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000021">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000115">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000017">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026283">
        <property type="entry name" value="B-gal_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048912">
        <property type="entry name" value="BetaGal1-like_ABD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048913">
        <property type="entry name" value="BetaGal_gal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019801">
        <property type="entry name" value="Glyco_hydro_35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21317">
        <property type="entry name" value="BetaGal_ABD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21467">
        <property type="entry name" value="BetaGal_gal-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006336">
        <property type="entry name" value="B-gal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01182">
        <property type="entry name" value="GLYCOSYL_HYDROL_F35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 16" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019042386" description="Beta-galactosidase" evidence="18">
        <location>
            <begin position="21"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase 35 catalytic" evidence="19">
        <location>
            <begin position="38"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase 1-like first all-beta" evidence="20">
        <location>
            <begin position="400"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase galactose-binding" evidence="21">
        <location>
            <begin position="541"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR006336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF21317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF21467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="658" mass="73610" checksum="6F9B7D6521C67E6C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I443</accession>
    <name>A0A452I443_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1</fullName>
            <ecNumber evidence="3">1.14.11.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Lysyl hydroxylase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-lysyl-[collagen] + 2-oxoglutarate + O2 = (5R)-5-hydroxy-L-lysyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:16569"/>
            <dbReference type="Rhea" id="RHEA-COMP:12751"/>
            <dbReference type="Rhea" id="RHEA-COMP:12752"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:133442"/>
            <dbReference type="EC" id="1.14.11.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Rough endoplasmic reticulum membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.14.11.4" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452I443"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022307"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025422.1">
        <property type="protein sequence ID" value="ENSGAGP00000022307.1"/>
        <property type="gene ID" value="ENSGAGG00000016382.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000025423.1">
        <property type="protein sequence ID" value="ENSGAGP00000022308.1"/>
        <property type="gene ID" value="ENSGAGG00000016382.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030867">
        <property type="term" value="C:rough endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008475">
        <property type="term" value="F:procollagen-lysine 5-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23004">
        <property type="entry name" value="GT_LH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000004">
        <property type="entry name" value="Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050757">
        <property type="entry name" value="Collagen_mod_GT25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057589">
        <property type="entry name" value="GT_PLOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001006">
        <property type="entry name" value="Procol_lys_dOase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730:SF5">
        <property type="entry name" value="PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730">
        <property type="entry name" value="PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE/GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25342">
        <property type="entry name" value="GT_PLOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25238">
        <property type="entry name" value="OGFOD2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01325">
        <property type="entry name" value="LYS_HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="17">
        <location>
            <begin position="705"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="796" mass="91187" checksum="1FFE8F4BB1C2813D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I483</accession>
    <name>A0A452I483_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Binds to MUC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I483"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022343"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025459.1">
        <property type="protein sequence ID" value="ENSGAGP00000022343.1"/>
        <property type="gene ID" value="ENSGAGG00000016400.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000025460.1">
        <property type="protein sequence ID" value="ENSGAGP00000022344.1"/>
        <property type="gene ID" value="ENSGAGG00000016400.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14831">
        <property type="entry name" value="AP1_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000009">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044733">
        <property type="entry name" value="AP1_sigma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="14">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="156" mass="18534" checksum="8F116A75E43B487F" modified="2019-05-08" version="1">MQFMLLFSRQGKLRLQKWYVPLSDKEKKKITRELVQTVLARKPKMCSFLEWRDLKIVYKRYASLYFCCAIEDQDNELITLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFILDEFLLGGEVQETSKKNVLKAIEQADLLQESQNEDWGGLSEDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4D1</accession>
    <name>A0A452I4D1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 512</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I4D1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022409"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025534.1">
        <property type="protein sequence ID" value="ENSGAGP00000022409.1"/>
        <property type="gene ID" value="ENSGAGG00000016445.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000177">
        <property type="entry name" value="Zinc finger protein 512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000270">
        <property type="entry name" value="Zinc finger protein 512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000580">
        <property type="entry name" value="Zinc finger protein 512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052274">
        <property type="entry name" value="Krueppel_C2H2_Zn-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048408">
        <property type="entry name" value="ZNF512_C2HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048403">
        <property type="entry name" value="ZNF512_znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22979:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN 512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22979">
        <property type="entry name" value="ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21276">
        <property type="entry name" value="ZNF512_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21367">
        <property type="entry name" value="ZNF512_zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="285"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="438"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="484"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="491"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="567" mass="65100" checksum="2005BD437794714D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6A1</accession>
    <name>A0A452I6A1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small monomeric GTPase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I6A1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023108"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026331.1">
        <property type="protein sequence ID" value="ENSGAGP00000023108.1"/>
        <property type="gene ID" value="ENSGAGG00000016932.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002500">
        <property type="entry name" value="Ras-related protein Rap-2a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16787" checksum="F61838B95215AAD3" modified="2019-05-08" version="1">MREYKVVVLGSGGVGKSALTVQFVTGSFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYEKVPMILVGNKVDLEGEREVSSQHFRNWRRGFGVWVVARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I882</accession>
    <name>A0A452I882_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000023854"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027170.1">
        <property type="protein sequence ID" value="ENSGAGP00000023854.1"/>
        <property type="gene ID" value="ENSGAGG00000017458.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="47"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="248" mass="28681" checksum="4C6EF9D3C8FE2BEA" modified="2019-05-08" version="1">MAGEFPSLLVLSQTMTERQWVWDARKAGDSAKVRETILRHYDMEMQRQRFRAFCYQEAEGPWEIYGHLWKLSHRCLQPETLTKEQMLELLVLEQFLSLLPEEMQSWVQQGGPERGEEAVALAEDFQLIHLQPWKHQSQLGTWEVSGXWRLVPRPVPQAPTPSASDLWTGNQVMKTPFWMGSWILWTPCCQLQLLPLLFPHLLLAPFESGTCFLDLSSFSSCTMMSLAMLHLLSMQVCDGSMPFPHYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I928</accession>
    <name>A0A452I928_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I928"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024050"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027391.1">
        <property type="protein sequence ID" value="ENSGAGP00000024050.1"/>
        <property type="gene ID" value="ENSGAGG00000017596.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001872">
        <property type="term" value="F:(1-&gt;3)-beta-D-glucan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071226">
        <property type="term" value="P:cellular response to molecule of fungal origin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042808">
        <property type="entry name" value="CLEC7A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47218">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 7 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47218:SF2">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="18248" checksum="1D05B3C705A5E215" modified="2019-05-08" version="1">MQQLKAFFKLCLYIVNHNLVLKDTFWCSSIVNLTFFISHQANRWQEKFTQQQEILENLTHQQEILGNLTQKLQEIQEKTSQEKEDLQAKNAELSKSLQIYSKDDWMQNGKDCYYFSTKIKTWFECKEYCSSLSSRLLKIDSKEELVESILLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I937</accession>
    <name>A0A452I937_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein bS6m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">28S ribosomal protein S6, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I937"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024060"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027402.1">
        <property type="protein sequence ID" value="ENSGAGP00000024060.1"/>
        <property type="gene ID" value="ENSGAGG00000017604.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15465">
        <property type="entry name" value="bS6_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000008">
        <property type="entry name" value="28S ribosomal protein S6, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_bS6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00166">
        <property type="entry name" value="S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS6M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="Ribosomal protein S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="121" mass="13942" checksum="7089CF116A4CC44C" modified="2019-05-08" version="1">MPRYELALILKATQRPEMASALKRTIEALMERGAVVRNLENLGERSLPYKISKHNQDHRRGGYFLVDFDGPPTIVSTMMDHLGRDVDVIRPTFLKHRVSKTEECSGIIPVNYEDKLYTKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB22</accession>
    <name>A0A452IB22_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Proprotein convertase subtilisin/kexin type 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Prohormone convertase 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Proprotein convertase 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Subtilisin/kexin-like protease PC7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Serine endoprotease that processes various proproteins by cleavage at paired basic amino acids, recognizing the RXXX[KR]R consensus motif. Likely functions in the constitutive secretory pathway.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IB22"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024932"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028379.1">
        <property type="protein sequence ID" value="ENSGAGP00000024932.1"/>
        <property type="gene ID" value="ENSGAGG00000018225.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000026">
        <property type="entry name" value="proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000002">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000005">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF28">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 21" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 21" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="P/Homo B" evidence="23">
        <location>
            <begin position="492"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="202"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20 21">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20 21">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20 21">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00076733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00076944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="677" mass="74627" checksum="5D86B45147E96136" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBJ4</accession>
    <name>A0A452IBJ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IBJ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025114"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028594.1">
        <property type="protein sequence ID" value="ENSGAGP00000025114.1"/>
        <property type="gene ID" value="ENSGAGG00000018370.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15911">
        <property type="entry name" value="7tmA_OR11A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="308" mass="34764" checksum="0AE008A1E3BE43EA" modified="2019-05-08" version="1">IDWGNQTAITEFIILGFRDLPDLQILPFLMFQVIYMATVAGNTLIVVLIVADLHFHTPMYFFLGNLSCLETCYTSAIQPRLLLVTGDKTISVSGCITQLYFCSSLVCTECYLLAAMPYDRYLAICKPLHYSALMNIRFCVQLTARSWLNGCLAVIILVSFLSQLLFCGPNEIDHFYCDPIPLMELSCSDTHLVILLAFIIASIFTLPPLLLTLTFYMCIISTILRIPSTTGRQKAFSTCSSHLIVVTIFYGSIMIVYLLPKRDTLRDLNKVLSLCYTVLTPLVNPLIYSLRNREVKEALCKGKYMHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICI6</accession>
    <name>A0A452ICI6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ICI6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025475"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028995.1">
        <property type="protein sequence ID" value="ENSGAGP00000025475.1"/>
        <property type="gene ID" value="ENSGAGG00000018620.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000007">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0552">Olfaction</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="42"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="313" mass="35280" checksum="21D3E74B48B1587A" modified="2019-05-08" version="1">MEQQNLSSTVTEFVLLGLTQNAELKQCLFVVFFIVYLTTWLGNFTIITTVITSHRLHIPMYFLLANLAFLDVSDSSVNAPKLLLGLLSQCRTISFNECILQMFFFHFIASAMVVVLVVMAADRYIAIYKPLRYLTIMNHGVCTGLVAGAWLGGLAHSAVQIALILQLPFCGPNFLDNFYCDTPQVIKLACKDIYLVELLMVSNSGMLVIVIFIILLISYIIIFVKIKTHVTDGKHKALSTCGSQITVVCLQFIPCIFIYARPFWKLPMDKLLSIIYTVITPMLNPMIYTLRNDEMKKAIRKLISRILSSGKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ID03</accession>
    <name>A0A452ID03_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Collagen IV NC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
            <location evidence="2">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000025391"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028899.1">
        <property type="protein sequence ID" value="ENSGAGP00000025391.1"/>
        <property type="gene ID" value="ENSGAGG00000018566.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.240.10:FF:000001">
        <property type="entry name" value="Collagen IV alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.240.10">
        <property type="entry name" value="Collagen IV, non-collagenous"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001442">
        <property type="entry name" value="Collagen_IV_NC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036954">
        <property type="entry name" value="Collagen_IV_NC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1112">
        <property type="entry name" value="COL_CUTICLE_N DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01413">
        <property type="entry name" value="C4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00111">
        <property type="entry name" value="C4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51403">
        <property type="entry name" value="NC1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019071825" description="Collagen IV NC1 domain-containing protein" evidence="10">
        <location>
            <begin position="36"/>
            <end position="1718"/>
        </location>
    </feature>
    <feature type="domain" description="Collagen IV NC1" evidence="11">
        <location>
            <begin position="1496"/>
            <end position="1718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="85"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="209"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="328"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="638"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="689"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="961"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1017"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1107"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1166"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1233"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1299"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="151"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="442"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="484"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="504"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="579"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="691"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="760"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="812"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="822"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="837"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1392"/>
            <end position="1401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1718" mass="170004" checksum="8DA841753A30431E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDV5</accession>
    <name>A0A452IDV5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nicotinamide N-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDV5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026011"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029586.1">
        <property type="protein sequence ID" value="ENSGAGP00000026011.1"/>
        <property type="gene ID" value="ENSGAGG00000019002.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000065">
        <property type="entry name" value="Phenylethanolamine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000940">
        <property type="entry name" value="NNMT_TEMT_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10867:SF32">
        <property type="entry name" value="NICOTINAMIDE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10867">
        <property type="entry name" value="NNMT/PNMT/TEMT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01234">
        <property type="entry name" value="NNMT_PNMT_TEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000384">
        <property type="entry name" value="PNMTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51681">
        <property type="entry name" value="SAM_MT_NNMT_PNMT_TEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="68"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="142"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000384-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="29408" checksum="BE1BD227CBD8552D" modified="2019-05-08" version="1">MCRLGGSTMAEFTGGDDYQADFDPKAYLRHYKFGEDTFGEDYLNFSLKHYCKTFTSGVVTGDTLIDIGSGPTIHQLLSACESFKEIIASDYTYRNHQELEKWLKNEPGAFDWTPVVKYVCEGKEAEKEAKLRKTIKQVLKCDVHKSNPMDPVILPPADCLVSSLCLEGACKDLTTYRIALKNISSLLKPGGHLVLSGVLGCSFYMVGPKRFSSLVLGEEFLREALSDTGFIIQEFEVLIRDDNTDDSCDFSGKFFILALGLQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDW0</accession>
    <name>A0A452IDW0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome P450 26B1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>all-trans-retinoate + reduced [NADPH--hemoprotein reductase] + O2 = all-trans-18-hydroxyretinoate + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55856"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35291"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:139258"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:55857"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>all-trans-retinoate + reduced [NADPH--hemoprotein reductase] + O2 = all-trans-4-hydroxyretinoate + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51984"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35291"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:134178"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:51985"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDW0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025884"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029442.1">
        <property type="protein sequence ID" value="ENSGAGP00000025884.1"/>
        <property type="gene ID" value="ENSGAGG00000018896.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034653">
        <property type="term" value="P:retinoic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20637">
        <property type="entry name" value="CYP26B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000009">
        <property type="entry name" value="Cytochrome P450 26B1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF177">
        <property type="entry name" value="CYTOCHROME P450 26B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 17 18" id="KW-0349">Heme</keyword>
    <keyword evidence="10 17 18" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 18" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="17">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="511" mass="57899" checksum="852C3A448F77681F" modified="2019-05-08" version="1">MLFESFDLVSALATLAACLVSLTLLLAVSQQLWQLRWAATRDKSCKLPIPKGSMGFPLIGETFHWLLQGSCFQSSRREKYGNVFKTHLLGRPLIRVTGAENVRKILMGEHHLVSTEWPRSTRMLLGPNTVANSIGDIHRHKRKVFSKIFSHEALESYLPKIQLVIQDTLRMWSSTPEPINVYYEAQKLTFRMAIRVLLGFCISDEDLTRLFEVYQQFVENVFSLPVDLPFSGYRRGIRARETLQKGLEKAIREKLQNTQGKDYSDALDILIESGKEHGKELTMQELKDGTLELIFAAYATTASASTSLIMQLLKHPRVLEKLREELRSKGILHNGCICEGSLRLDTISSLHYLDCVIKEVLRLFTPISGGYRTVLQTFELDGFQIPKGWSVMYSIRDTHDTAPVFKDVDVFDPDRFGQDRTEDKDGRFHYLPFGGGVRTCLGKHLAKLFLKALAIELASTSRFELATRTFPRITLVPVVHPVDGLKVKFFGLDSNQNEILTETEAMLGATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IE91</accession>
    <name>A0A452IE91_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Solute carrier family 7 member 13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IE91"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029595.1">
        <property type="protein sequence ID" value="ENSGAGP00000026020.1"/>
        <property type="gene ID" value="ENSGAGG00000019006.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000036">
        <property type="entry name" value="Solute carrier family 7 member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF348">
        <property type="entry name" value="ASC-TYPE AMINO ACID TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="54410" checksum="84B3E3E45F1609E6" modified="2019-05-08" version="1">LGEKKMGKGKHDYLKEEVRGNENDRMQLKRTIGYFDGVNFIIGTIVGAGIFVSPTGVLQYSLLNVGVALSIWTACGVISLMGALCYAELGTALPLSGGEYSHIKRALGSLPAFIFIWTAMFTKPASNATRALLFAEYATQPFYGVCHAPELLKKCLALTVLWSLGILNGRSVKMATWVQTVFTVLKMMALSVIGIGGIVLLVRGRKENLVRFENAFSSEIPDASQIAEAFFQGLYAYGGWWSLNYMAEEMINPSRNIPLTVMTALPAVTLFYLLVNISYLTVLTPKEIVSSVAVAVTWADRVIPSVAWVIPVSVAASIFGALNSSMFTLGRLSYAGSQSGHLPVLISMLNVHYWTPAPAMIFSTIIASIFIIPADLITLTNYFGFSVWLMVGLTCASLIVLRYREPTLQRPYKVFLPVAFVMVAVSLFLVLAPIVWAPKMQYIYALLFMLGGLLVYLPFVHFKLHFDFVDKITCYLQLLLEVSPADVSAEDKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IEG0</accession>
    <name>A0A452IEG0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">RING-type E3 ubiquitin transferase BRCA1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="37 38">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="38" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="37" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="37" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 39">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026218"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029815.1">
        <property type="protein sequence ID" value="ENSGAGP00000026218.1"/>
        <property type="gene ID" value="ENSGAGG00000019135.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070531">
        <property type="term" value="C:BRCA1-A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031436">
        <property type="term" value="C:BRCA1-BARD1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070013">
        <property type="term" value="C:intracellular organelle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17735">
        <property type="entry name" value="BRCT_BRCA1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17721">
        <property type="entry name" value="BRCT_BRCA1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16498">
        <property type="entry name" value="RING-HC_BRCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000213">
        <property type="entry name" value="Breast cancer type 1 susceptibility protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000006">
        <property type="entry name" value="Breast cancer type 1 susceptibility protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000025">
        <property type="entry name" value="Breast cancer type 1 susceptibility protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011364">
        <property type="entry name" value="BRCA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031099">
        <property type="entry name" value="BRCA1-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025994">
        <property type="entry name" value="BRCA1_serine_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13763:SF0">
        <property type="entry name" value="BREAST CANCER TYPE 1 SUSCEPTIBILITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13763">
        <property type="entry name" value="BREAST CANCER TYPE 1 SUSCEPTIBILITY PROTEIN BRCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12820">
        <property type="entry name" value="BRCT_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001734">
        <property type="entry name" value="BRCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00493">
        <property type="entry name" value="BRSTCANCERI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="20" id="KW-0007">Acetylation</keyword>
    <keyword evidence="24" id="KW-0010">Activator</keyword>
    <keyword evidence="30" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="33" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="27" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="28" id="KW-0234">DNA repair</keyword>
    <keyword evidence="23" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="25" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="17" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="22" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="29" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="38" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="26" id="KW-0804">Transcription</keyword>
    <keyword evidence="21" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="19" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="16" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="18" id="KW-0862">Zinc</keyword>
    <keyword evidence="15 32" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="35">
        <location>
            <begin position="24"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="36">
        <location>
            <begin position="1698"/>
            <end position="1782"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="36">
        <location>
            <begin position="1802"/>
            <end position="1900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="318"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="413"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="496"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="660"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="966"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1020"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1219"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1357"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1510"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="33">
        <location>
            <begin position="1426"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="34">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="384"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="34">
        <location>
            <begin position="539"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="609"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="34">
        <location>
            <begin position="627"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="34">
        <location>
            <begin position="674"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="34">
        <location>
            <begin position="698"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="34">
        <location>
            <begin position="1024"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="34">
        <location>
            <begin position="1224"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="1363"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="34">
        <location>
            <begin position="1381"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="34">
        <location>
            <begin position="1523"/>
            <end position="1535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00031556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1903" mass="211202" checksum="B65A3BD243FAF860" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFX9</accession>
    <name>A0A452IFX9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 20">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="14">IMP dehydrogenase</shortName>
            <shortName evidence="14">IMPD</shortName>
            <shortName evidence="14">IMPDH</shortName>
            <ecNumber evidence="14 20">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">IMPDH</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14 20">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12 14 20">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14 19">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="14 20"/>
    <dbReference type="AlphaFoldDB" id="A0A452IFX9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026381"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029993.1">
        <property type="protein sequence ID" value="ENSGAGP00000026381.1"/>
        <property type="gene ID" value="ENSGAGG00000019229.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000007">
        <property type="entry name" value="Chromosome 19 SCAF14664, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF121">
        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0129">CBS domain</keyword>
    <keyword evidence="2 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 14 20" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="3 14 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14 16" id="KW-0520">NAD</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 14 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 14 17" id="KW-0630">Potassium</keyword>
    <keyword evidence="6 14 20" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="21">
        <location>
            <begin position="80"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="21">
        <location>
            <begin position="145"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="14 15">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14 15">
        <location>
            <position position="395"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <begin position="240"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <begin position="290"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14 17">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14 17">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14 17">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="330"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="353"/>
            <end position="354"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="377"/>
            <end position="381"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="515" mass="55439" checksum="9B8753E820232233" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGQ2</accession>
    <name>A0A452IGQ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IGQ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026944"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030624.1">
        <property type="protein sequence ID" value="ENSGAGP00000026944.1"/>
        <property type="gene ID" value="ENSGAGG00000019615.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF133">
        <property type="entry name" value="RIKEN CDNA 1700020N01 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="13"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="65" mass="7510" checksum="5064ABE9A1BD5BAA" modified="2019-05-08" version="1">MDFVGLLLFQGPVTFEVVAVYFTREQWALLDPTQRALYRDVMQENYDNVTSLGKDSHPLSSWKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIG1</accession>
    <name>A0A452IIG1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">General transcription factor II-I repeat domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">May be a transcription regulator involved in cell-cycle progression and skeletal muscle differentiation. May repress GTF2I transcriptional functions, by preventing its nuclear residency, or by inhibiting its transcriptional activation. May contribute to slow-twitch fiber type specificity during myogenesis and in regenerating muscles. Binds troponin I slow-muscle fiber enhancer (USE B1). Binds specifically and with high affinity to the EFG sequences derived from the early enhancer of HOXC8.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the retinoblastoma protein (RB1) via its C-terminus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IIG1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027651"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031414.1">
        <property type="protein sequence ID" value="ENSGAGP00000027651.1"/>
        <property type="gene ID" value="ENSGAGG00000020096.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000031425.1">
        <property type="protein sequence ID" value="ENSGAGP00000027659.1"/>
        <property type="gene ID" value="ENSGAGG00000020096.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000031428.1">
        <property type="protein sequence ID" value="ENSGAGP00000027662.1"/>
        <property type="gene ID" value="ENSGAGG00000020096.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1460.10:FF:000002">
        <property type="entry name" value="General transcription factor II-I isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1460.10:FF:000005">
        <property type="entry name" value="general transcription factor II-I repeat domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1460.10:FF:000006">
        <property type="entry name" value="General transcription factor II-I repeat domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1460.10">
        <property type="entry name" value="GTF2I-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004212">
        <property type="entry name" value="GTF2I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036647">
        <property type="entry name" value="GTF2I-like_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016659">
        <property type="entry name" value="TF_II-I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46304">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I REPEAT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46304:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I REPEAT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02946">
        <property type="entry name" value="GTF2I"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016441">
        <property type="entry name" value="TF_II-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117773">
        <property type="entry name" value="GTF2I-like repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51139">
        <property type="entry name" value="GTF2I"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="434"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="510"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="650"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="899"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="442"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="921"/>
            <end position="937"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="968" mass="108131" checksum="3D02DA8446533A0A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJG6</accession>
    <name>A0A452IJG6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJG6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027941"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031734.1">
        <property type="protein sequence ID" value="ENSGAGP00000027941.1"/>
        <property type="gene ID" value="ENSGAGG00000020289.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000012">
        <property type="entry name" value="Zinc finger MIZ domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057847">
        <property type="entry name" value="ZMIZ1/ZMIZ2_GBD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF38">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25527">
        <property type="entry name" value="GBD-like_ZMIZ1_ZMIZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="10">
        <location>
            <begin position="614"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="276"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="756"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="768"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="812"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="847"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="901"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="933"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="971" mass="102566" checksum="D68D8E2E68A5CAD0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJI5</accession>
    <name>A0A452IJI5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Surfactant protein C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pulmonary surfactant-associated protein C</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Surface film</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJI5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028078"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031905.1">
        <property type="protein sequence ID" value="ENSGAGP00000028078.1"/>
        <property type="gene ID" value="ENSGAGG00000020400.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007585">
        <property type="term" value="P:respiratory gaseous exchange by respiratory system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007084">
        <property type="entry name" value="BRICHOS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001729">
        <property type="entry name" value="SP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015091">
        <property type="entry name" value="Surfactant_protein_propep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10800">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10800:SF4">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04089">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08999">
        <property type="entry name" value="SP_C-Propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01039">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00019">
        <property type="entry name" value="SF_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50869">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0305">Gaseous exchange</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0767">Surface film</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="BRICHOS" evidence="12">
        <location>
            <begin position="96"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="185" mass="20733" checksum="8BFB3A6513365946" modified="2019-05-08" version="1">MDVSSKEALIEAPPDYTPIPRIPCLSPHLKRLLIIMVVVVIIVLVVLGFLLMGLHISEKHTETVLRMTIHGLDGEGSLQQLSMSGKDRTGTFHIKAAINSSATVVYDYHHLLICYKSWQGRACYITKMDKENIQGLDTIAKMFQHFQLEQGEEMEEVGFPVPQANRSILGTTINILCSNVPIYWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IL19</accession>
    <name>A0A452IL19_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">acylaminoacyl-peptidase</fullName>
            <ecNumber evidence="5">3.4.19.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S9C family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452IL19"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028522"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032393.1">
        <property type="protein sequence ID" value="ENSGAGP00000028522.1"/>
        <property type="gene ID" value="ENSGAGG00000020665.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000043">
        <property type="entry name" value="acylamino-acid-releasing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045550">
        <property type="entry name" value="AARE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF4">
        <property type="entry name" value="ACYLAMINO-ACID-RELEASING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19283">
        <property type="entry name" value="APEH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylamino-acid-releasing enzyme N-terminal" evidence="9">
        <location>
            <begin position="321"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="8">
        <location>
            <begin position="550"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF19283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="758" mass="81340" checksum="8F7B948C131AF6D5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILD0</accession>
    <name>A0A452ILD0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C65 family. Otulin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILD0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028639"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032531.1">
        <property type="protein sequence ID" value="ENSGAGP00000028639.1"/>
        <property type="gene ID" value="ENSGAGG00000020756.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023235">
        <property type="entry name" value="FAM105"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023236">
        <property type="entry name" value="OTULINL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33662:SF1">
        <property type="entry name" value="INACTIVE UBIQUITIN THIOESTERASE OTULINL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33662">
        <property type="entry name" value="OTU DEUBIQUITINASE WITH LINEAR LINKAGE-SPECIFICITY A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16218">
        <property type="entry name" value="Peptidase_C101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02055">
        <property type="entry name" value="PROTEINF105"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02056">
        <property type="entry name" value="PROTEINF105A"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="311" mass="37431" checksum="9906D27B9570D532" modified="2019-05-08" version="1">ILVMSFLLAALWYCRRLYAYLAQLLKWWSRYLQRKFKKNLSVLAEVDLLGYCAREWKGETKQAKQMKAAYEELFWRHHVKYIRQVRRDNYCALRAVLFQVFSKGIYFPSWMKERDILKLPEKLLYSQGCNWIQQYSFGPERYTGANVFGKLRKCIEILKTQWTEISGVKDHDERGNMCNILFSDESKEHKLYEAIKFIMLYQVVEAYEQMKNEQKRVPILFSLLFARDTSSDPLSFMMNHLNSIGDTGGLEQVEMFLLGYLLEVKIRVYRLYKFNTEEFQVNYPEEYQRDWQEVSLLTEDDHHYLIPIIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMN3</accession>
    <name>A0A452IMN3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IMN3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029097"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033053.1">
        <property type="protein sequence ID" value="ENSGAGP00000029097.1"/>
        <property type="gene ID" value="ENSGAGG00000021053.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="57"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="317" mass="35803" checksum="5D52AB9F6C9E406E" modified="2019-05-08" version="1">MINGNVDMEVDITATEVEAVLEQLDGTKSEGPDNLHPRILKELAREIASPLARIFKQSINSGVVPYDWRIANVVPIFKKGNKSDPGNYRPVSLTSVVCKVLEKILREKVVKDIEVNGNWDELQHGFTKGRSCQTNLISFFEKVTDYLDKGNAVDLIYLDFSKAFDTVPHGELLVKLEKMGMNMKVVRWIRNWLKGRLQRVVLKGELSGWKEVTSGVPQGSVLGPILFNLFITDLGTKSGNVLIKFADDTKLGGIANTEKDRDTIQEDLDHLVNWSNSNRMKYNSEKCKVMHLGINNKNFRYTLGTHQLEATEQEKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQJ4</accession>
    <name>A0A452IQJ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IQJ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030230"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034311.1">
        <property type="protein sequence ID" value="ENSGAGP00000030230.1"/>
        <property type="gene ID" value="ENSGAGG00000021795.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000034314.1">
        <property type="protein sequence ID" value="ENSGAGP00000030233.1"/>
        <property type="gene ID" value="ENSGAGG00000021795.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000034320.1">
        <property type="protein sequence ID" value="ENSGAGP00000030238.1"/>
        <property type="gene ID" value="ENSGAGG00000021795.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09929">
        <property type="entry name" value="SH2_BLNK_SLP-76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000016">
        <property type="entry name" value="B-cell linker protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051751">
        <property type="entry name" value="Immunoreceptor_sig_adapters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14098:SF3">
        <property type="entry name" value="B-CELL LINKER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14098">
        <property type="entry name" value="SH2 DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="4">
        <location>
            <begin position="511"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="123"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="233"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="255"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="342"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="377"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="621" mass="69742" checksum="FA4C19F11D4985A8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IR12</accession>
    <name>A0A452IR12_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell junction</location>
            <location evidence="5">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell projection</location>
            <location evidence="10">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell projection</location>
            <location evidence="8">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
            <location evidence="9">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Membrane</location>
            <location evidence="11">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IR12"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030413"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034512.1">
        <property type="protein sequence ID" value="ENSGAGP00000030413.1"/>
        <property type="gene ID" value="ENSGAGG00000021900.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11959">
        <property type="entry name" value="SH3_Cortactin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000087">
        <property type="entry name" value="Src substrate cortactin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035716">
        <property type="entry name" value="Cortactin_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003134">
        <property type="entry name" value="Hs1_Cortactin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF15">
        <property type="entry name" value="SRC SUBSTRATE CORTACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02218">
        <property type="entry name" value="HS1_rep"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51090">
        <property type="entry name" value="CORTACTIN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="19" id="KW-0965">Cell junction</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="25" id="KW-0966">Cell projection</keyword>
    <keyword evidence="23" id="KW-0168">Coated pit</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="24" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="16" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="22" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 26" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="28" id="KW-0732">Signal</keyword>
    <keyword evidence="20" id="KW-0770">Synapse</keyword>
    <feature type="signal peptide" evidence="28">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019225997" description="SH3 domain-containing protein" evidence="28">
        <location>
            <begin position="20"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="29">
        <location>
            <begin position="468"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="326"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="329"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="526" mass="59686" checksum="8B65867C7FABA964" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IS11</accession>
    <name>A0A452IS11_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IS11"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030645"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034768.1">
        <property type="protein sequence ID" value="ENSGAGP00000030645.1"/>
        <property type="gene ID" value="ENSGAGG00000022055.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04037">
        <property type="entry name" value="C2E_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08374">
        <property type="entry name" value="C2F_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037724">
        <property type="entry name" value="C2E_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037725">
        <property type="entry name" value="C2F_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037721">
        <property type="entry name" value="Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032362">
        <property type="entry name" value="Ferlin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055072">
        <property type="entry name" value="Ferlin_DSRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546">
        <property type="entry name" value="FER-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546:SF34">
        <property type="entry name" value="FER-1-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22901">
        <property type="entry name" value="dsrm_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16165">
        <property type="entry name" value="Ferlin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="725"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="232"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="468"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="792" mass="89681" checksum="01165F5F582E4D16" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ISJ7</accession>
    <name>A0A452ISJ7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ISJ7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031089"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035254.1">
        <property type="protein sequence ID" value="ENSGAGP00000031089.1"/>
        <property type="gene ID" value="ENSGAGG00000022340.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000016">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF116">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="2">
        <location>
            <begin position="59"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="3">
        <location>
            <begin position="212"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="533" mass="58764" checksum="D8C9AE167B224FCE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IW33</accession>
    <name>A0A452IW33_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IW33"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032232"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036538.1">
        <property type="protein sequence ID" value="ENSGAGP00000032232.1"/>
        <property type="gene ID" value="ENSGAGG00000023070.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="164"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="295"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="440" mass="49707" checksum="9322424880F73A1A" modified="2019-05-08" version="1">MNFRIYSAVYCFLCTGILKHHNGVATAGSTFSQSRQLSCLFETTETIPLLSEFKYIRQNAWLLLSGTERPHQENPPAADTLTFIVQESSLDILQHMDEDTDQLECKISRYFTDNTQILWPGLQTQLHKLDSWFTGTIRHLADKFTVTVFLVQSSASKEAENQVPEDVSQGNAERFYLSGVFLVRTGPSLIQSGLNKDVLLSCAFSVDHHTDVTIKWVFQQKGGHKKLIFAYNGFTRQVEHKDKWAEMFLAEMPKGNASLLLRTVGVRDAGTYSCSILVSSLLWAQNVQLEIVEKPTVTLNADVLSLVEGGEQKLVCDISHFYPHDADAQWLQEPVEQGMLPHVVTHVVSSSHRHNSDGTYSFSSYFLLKASLRDDGRRYTCRVEHQSLKHPIKKSLTVKVTESTSATWFLVILLVLLAGCLVVMLYYLYRGMSANKPKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IXW0</accession>
    <name>A0A452IXW0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphodiesterase</fullName>
            <ecNumber evidence="7">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="7">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452IXW0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032904"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037294.1">
        <property type="protein sequence ID" value="ENSGAGP00000032904.1"/>
        <property type="gene ID" value="ENSGAGG00000023451.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000009">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000007">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0140">cGMP</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="8">
        <location>
            <begin position="529"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="544"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="544"/>
            <end position="548"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="548"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="584"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="585"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="585"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="585"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="696"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="696"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="747"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="835" mass="92939" checksum="E14E2DDCB5F2EC41" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IY43</accession>
    <name>A0A452IY43_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Epithelial membrane protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="15">Interacts with PTK2; regulates PTK2 activation and localization. Interacts with ITGB3; regulates the levels of the heterodimer ITGA5-ITGB3 integrin surface expression. Interacts with P2RX7 (via C-terminus). Interacts with ITGB1; the interaction may be direct or indirect and ITGB1 has a heterodimer form.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 16">Belongs to the PMP-22/EMP/MP20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IY43"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032994"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037409.1">
        <property type="protein sequence ID" value="ENSGAGP00000032994.1"/>
        <property type="gene ID" value="ENSGAGG00000023566.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001046">
        <property type="term" value="P:positive regulation of integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001952">
        <property type="term" value="P:regulation of cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000023">
        <property type="entry name" value="Epithelial membrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003933">
        <property type="entry name" value="EMP-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050579">
        <property type="entry name" value="PMP-22/EMP/MP20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004032">
        <property type="entry name" value="PMP22_EMP_MP20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF32">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01453">
        <property type="entry name" value="EPMEMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01455">
        <property type="entry name" value="EPMEMPROT2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01222">
        <property type="entry name" value="PMP22_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="10 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019411436" description="Epithelial membrane protein 2" evidence="17">
        <location>
            <begin position="17"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="141"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="170" mass="19273" checksum="BE76C01E953641ED" modified="2019-05-08" version="1">MLILLAFIIVFHITSAALLFISTIDNVSFIPPTNSNSEPYVSPCYTDSTAFTIKPAFIFTEYQSVQAVQATMILSTILCCVAFLVFILQLFRLKQGERFVLTSTIQLLSCLCVMIAASIYTNRHEDLHKSYGYMFDIHSGQYGYSFILAWIAFAFTLISGIMYLVLRKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZ07</accession>
    <name>A0A452IZ07_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase III subunit RPC7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPC7 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZ07"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033326"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037764.1">
        <property type="protein sequence ID" value="ENSGAGP00000033326.1"/>
        <property type="gene ID" value="ENSGAGG00000023758.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024661">
        <property type="entry name" value="RNA_pol_III_Rpc31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15367">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15367:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11705">
        <property type="entry name" value="RNA_pol_3_Rpc31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="11390" checksum="3886771EB3577828" modified="2019-05-08" version="1">MAGRGGRGRGRSQMTFNVEAVGIGKGDSLPPSTLQPSPLFPPMEYKPVPLQTGEEVEYMLALKQELRGAMKNLPYYVKPAAPKKENKQSLTCACCSHKSSYLASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0H7</accession>
    <name>A0A452J0H7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tektin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Sperm-specific microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in flagellar axoneme. Forms an extensive interaction network in different conformations that reinforces the helix bundle composed by other tektin proteins (TEKT1 to TEKT4) and MIPs to anchor the tektin bundle onto the tubulin wall of A-tubule of the sperm flagellum.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Microtubule inner protein component of sperm flagellar doublet microtubules. Interacts with TEKT3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
            <location evidence="12">Cytoskeleton</location>
            <location evidence="12">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the tektin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J0H7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033859"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038350.1">
        <property type="protein sequence ID" value="ENSGAGP00000033859.1"/>
        <property type="gene ID" value="ENSGAGG00000024073.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048256">
        <property type="entry name" value="Tektin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000435">
        <property type="entry name" value="Tektins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960:SF23">
        <property type="entry name" value="TEKTIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03148">
        <property type="entry name" value="Tektin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00511">
        <property type="entry name" value="TEKTIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7 12" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 12" id="KW-0282">Flagellum</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="522" mass="60126" checksum="F134DA069FF65721" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0Q1</accession>
    <name>A0A452J0Q1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutaminyl-peptide cyclotransferase</fullName>
            <ecNumber evidence="4">2.3.2.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Glutaminyl cyclase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Glutaminyl-tRNA cyclotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-terminal L-glutaminyl-[peptide] = N-terminal 5-oxo-L-prolyl-[peptide] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23652"/>
            <dbReference type="Rhea" id="RHEA-COMP:11736"/>
            <dbReference type="Rhea" id="RHEA-COMP:11846"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:64722"/>
            <dbReference type="ChEBI" id="CHEBI:87215"/>
            <dbReference type="EC" id="2.3.2.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutaminyl-peptide cyclotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.5" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452J0Q1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033886"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038378.1">
        <property type="protein sequence ID" value="ENSGAGP00000033886.1"/>
        <property type="gene ID" value="ENSGAGG00000024105.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016603">
        <property type="term" value="F:glutaminyl-peptide cyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03880">
        <property type="entry name" value="M28_QC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000029">
        <property type="entry name" value="Glutaminyl-peptide cyclotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037457">
        <property type="entry name" value="M28_QC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040234">
        <property type="entry name" value="QC/QCL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283:SF5">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M28" evidence="16">
        <location>
            <begin position="139"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="379" mass="43189" checksum="71956692AE80B1F9" modified="2019-05-08" version="1">MHRKKSSSSCRRGMAGGSRHNKAAASFCLAWALYLATITFSQLADGREARADWTQEKYFHQPSILNISGIQQVAAHTDVSQMWQNDLHPILIERYPGSPGSYIVRQHIKRRIQRLQAGWEVEEDTFQGYTPYGYRTFSNIISTLNPSAKRHLVLACHHDSKYFGPQWDGRVFVGATDSAVPCAMLLELAHALDSQLQSIKTSSASRPDLSLQLIFFDGEEAFVRWSPTDSLYGSRHLAQKMESTPHPPGATNTNQLQSIDLFVLLDLIGASNPTFPNYFSNTARWFNRLQAIERKLHGLGLLKNHRSERQYFRGNATPRSIEDDHIPFLRRGVQILHLIPSPFPKVWHTMEDNEQNLHKPTVDNLNKILQVFVLEYLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1B4</accession>
    <name>A0A452J1B4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KATNIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000034105"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038617.1">
        <property type="protein sequence ID" value="ENSGAGP00000034105.1"/>
        <property type="gene ID" value="ENSGAGG00000024234.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026704">
        <property type="entry name" value="KATNIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027859">
        <property type="entry name" value="KATNIP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21534">
        <property type="entry name" value="KATANIN-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21534:SF0">
        <property type="entry name" value="KATANIN-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14652">
        <property type="entry name" value="DUF4457"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KATNIP" evidence="2">
        <location>
            <begin position="369"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="KATNIP" evidence="2">
        <location>
            <begin position="820"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="domain" description="KATNIP" evidence="2">
        <location>
            <begin position="1106"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1511" mass="171181" checksum="AC9405AE1F21094C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J3W9</accession>
    <name>A0A452J3W9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E3 ubiquitin-protein transferase MAEA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Macrophage erythroblast attacher</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J3W9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035124"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039771.1">
        <property type="protein sequence ID" value="ENSGAGP00000035124.1"/>
        <property type="gene ID" value="ENSGAGG00000024970.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043249">
        <property type="term" value="P:erythrocyte maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16659">
        <property type="entry name" value="RING-Ubox_Emp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045098">
        <property type="entry name" value="Fyv10_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044063">
        <property type="entry name" value="ZF_RING_GID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN TRANSFERASE MAEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170">
        <property type="entry name" value="MACROPHAGE ERYTHROBLAST ATTACHER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51867">
        <property type="entry name" value="ZF_RING_GID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0265">Erythrocyte maturation</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CTLH" evidence="11">
        <location>
            <begin position="159"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="RING-Gid-type" evidence="12">
        <location>
            <begin position="352"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="RING-Gid-type" evidence="10">
        <location>
            <begin position="352"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="434" mass="49697" checksum="833D6C386A5FDFEF" modified="2019-05-08" version="1">MAVQESAAQLSMTLKVQEYPTLKVPYETLNKRFRAAQKNIDRETSHVTMVVAELEKTLSSCPAVDSVVSLLDGVVEKLSVLKRKAVESIQAEDESAKLCKRRIEHLKEHSSDQPAAANMWKKKRMDRMMVEHLLRCGYYNTAVKLARQSGIEDLVNIEMFLTAKEVEESLERQETMTCLAWCHDNKSRLRKMKGRQNENEPKMGRKSKSACDYSKENDDLVMETIKGKPELSCLEFSLRIQEFIELIRQNKRLDAVRHARKHFSQAEGSQLDEVRQVMGMLAFPSDTHISPYKDLLDPARWRMLIQQFRYDNYRLHQLGNNSVFTITLQAGLSAIKTPQCYKEDGSSKNPDCPVCSKSLNKLAQPLPMAHCANSRLVCKISGDVMNENNPPMMLPNGYVYGYNSLLSIRQDDKVICPRTKEVFNFSQAEKVYIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J420</accession>
    <name>A0A452J420_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MEIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J420"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035177"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039830.1">
        <property type="protein sequence ID" value="ENSGAGP00000035177.1"/>
        <property type="gene ID" value="ENSGAGG00000024999.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032453">
        <property type="entry name" value="PKNOX/Meis_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16493">
        <property type="entry name" value="Meis_PKNOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MEIS N-terminal" evidence="3">
        <location>
            <begin position="69"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="265" mass="28931" checksum="322F4BD49B8EBC21" modified="2019-05-08" version="1">MQYDDLPHYGGMDGVGIPTTMYGDPHAARSMQPVHHLNHGPPLHSHQYPHTAHTNAMPPSMGSSVNDALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQIRAEKPLFSSNPELDNLVRVNSALFYFSVLWGEVVHELCDNFCHRYISCLKGKMPIDLVIDDREGGSKSDSEDITRSANLTDQHTSLPSPPGPAAVLRGWGCRLERRAAGGRGCTRTTERRGKGRGRIVGTGGRRVGECV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4J5</accession>
    <name>A0A452J4J5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J4J5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035370"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040048.1">
        <property type="protein sequence ID" value="ENSGAGP00000035370.1"/>
        <property type="gene ID" value="ENSGAGG00000025139.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000478">
        <property type="entry name" value="Spectrin repeat-containing, nuclear envelope 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514:SF3">
        <property type="entry name" value="NESPRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514">
        <property type="entry name" value="PKA ANCHORING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="453" mass="52221" checksum="E37C4A9ED0DE536A" modified="2019-05-08" version="1">HLNLYWLYELTNIPIALAPFTQAGGAEKESLHVEQKLYDSVAATSSWLDGVEEGLFVATALLPEETETCLHNQESLAKDIKEMSEEMDKIKNLFSQTFPENSDNRDVIEDTLDCLLRRLTMLESVVNQRCYQMKERLQQILTFQNDLKLLFTSLADHKYIVLQKLAEAAERPEMEQMKAILQAEDGLKELDAGISKLKKHGDKLQIDQPSMQELSKLQDMYDELMMIVGSRRSGLNQNLALKGQYEQALQDLADLIETGQEKMAGDQKIIVASKEEVQMLLDKHKEYFQGLESHMILTETLFRKISSFALLRETQSHTKQMAQASAVLKQAHKRGVELEYILETWTHLDEDYQELTRQLEAVESNIPSVGLVEETEERLTDRITLYQRLKSSLTEYQPKLYQVLDDGKRLLFSISCSTLESQLNQLGEHWLNNTSKVTKELHRLETILKHWTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4K0</accession>
    <name>A0A452J4K0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Soluble calcium-activated nucleotidase 1</fullName>
            <ecNumber evidence="6">3.6.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Apyrase homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a ribonucleoside 5'-diphosphate + H2O = a ribonucleoside 5'-phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36799"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the apyrase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.6" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452J4K0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035376"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040054.1">
        <property type="protein sequence ID" value="ENSGAGP00000035376.1"/>
        <property type="gene ID" value="ENSGAGG00000025143.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.100:FF:000001">
        <property type="entry name" value="Soluble calcium-activated nucleotidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.100">
        <property type="entry name" value="Apyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009283">
        <property type="entry name" value="Apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036258">
        <property type="entry name" value="Apyrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13023">
        <property type="entry name" value="APYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13023:SF3">
        <property type="entry name" value="SOLUBLE CALCIUM-ACTIVATED NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06079">
        <property type="entry name" value="Apyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101887">
        <property type="entry name" value="Apyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR609283-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="402" mass="44604" checksum="1CED760D285D568B" modified="2019-05-08" version="1">MPVLPSQRLEWSESMTPLRISVGGLPVLASMTKAADPRFRLRWKAILVSSLSVALVLLLVCFHRSATGRPAPANAHNWRLSLLPGDRYNDTYPLSPPQRNSEGVRYRIGVIADLDTGSKGPQEHTWFSYLKKGYLTLSASGDRVTVEWDRADNLLESHLAEKGRGMELSELVVFNGKLYAVDDRTGVVYLIESTAVVPWVILSDGDGTVGKGFKAEWLAVKDEHLYVGGLGKEWTTTTGEVMNENPEWVKVVGSKGNVGHENWVSNYKALRAAAGISPPGYLIHESAAWSETLQRWFFLPRRASQERYNEKADEHRGTNLLLRATQDFGDIAVSHVGAAVPTHGFSSFKFIPDTDDQLIVALKSEEDNGKIATYIMAFTLDGRFLLPETRIGAVKYEGIEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4M0</accession>
    <name>A0A452J4M0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J4M0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035403"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040083.1">
        <property type="protein sequence ID" value="ENSGAGP00000035403.1"/>
        <property type="gene ID" value="ENSGAGG00000025162.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038039">
        <property type="term" value="C:G protein-coupled receptor heterodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15294">
        <property type="entry name" value="7tmC_GABA-B-R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06366">
        <property type="entry name" value="PBP1_GABAb_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000072">
        <property type="entry name" value="Gamma-aminobutyric acid type B receptor subunit 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041689">
        <property type="entry name" value="GBR2_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002457">
        <property type="entry name" value="GPCR_3_GABA_rcpt_B2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF74">
        <property type="entry name" value="GAMMA-AMINOBUTYRIC ACID TYPE B RECEPTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18455">
        <property type="entry name" value="GBR2_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01178">
        <property type="entry name" value="GABAB2RECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="434"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="477"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="510"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="552"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="610"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="651"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="673"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="19">
        <location>
            <begin position="436"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="718"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="817"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="728"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="817"/>
            <end position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="896" mass="101569" checksum="ED64D8AD36E285C4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5K2</accession>
    <name>A0A452J5K2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serotonin N-acetyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Aralkylamine N-acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2-arylethylamine + acetyl-CoA = an N-acetyl-2-arylethylamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20497"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:55469"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:77827"/>
            <dbReference type="EC" id="2.3.1.87"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the acetyltransferase family. AANAT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.87" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452J5K2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035740"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040467.1">
        <property type="protein sequence ID" value="ENSGAGP00000035740.1"/>
        <property type="gene ID" value="ENSGAGG00000025374.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004059">
        <property type="term" value="F:aralkylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030187">
        <property type="term" value="P:melatonin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009416">
        <property type="term" value="P:response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000021">
        <property type="entry name" value="Serotonin N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051635">
        <property type="entry name" value="SNAT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10908">
        <property type="entry name" value="SEROTONIN N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10908:SF0">
        <property type="entry name" value="SEROTONIN N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0471">Melatonin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="33"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="205" mass="23513" checksum="7CDBE25365D8F7E5" modified="2019-05-08" version="1">MSVISALPFLKPIHQRPPRSSPGRQRRHTLPASEFRCLTPEDAISVFEIEREAFISVSGDCPLHLDEIRHFLNLCPELSLGWFEEGRLVAFIIGSLWDQEKLSQDALTLHKPGGTTVHIHALAVHRTFRQQGKGSILMWRYLQYLRCLPYVRRAVLMCEDFLTPFYRKCGFKVQGPCEVTVGSLAFIEMQYPVRGHAFMRRNSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5N7</accession>
    <name>A0A452J5N7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTC21 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000035798"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040527.1">
        <property type="protein sequence ID" value="ENSGAGP00000035798.1"/>
        <property type="gene ID" value="ENSGAGG00000025406.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030991">
        <property type="term" value="C:intraciliary transport particle A"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000279">
        <property type="entry name" value="Tetratricopeptide repeat domain 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000219">
        <property type="entry name" value="Tetratricopeptide repeat domain 21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000245">
        <property type="entry name" value="Tetratricopeptide repeat domain 21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000414">
        <property type="entry name" value="Tetratricopeptide repeat domain 21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:003438">
        <property type="entry name" value="Tetratricopeptide repeat domain 21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056832">
        <property type="entry name" value="ARM_TT21_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056836">
        <property type="entry name" value="ARM_TT21_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056835">
        <property type="entry name" value="ARM_TT21_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056834">
        <property type="entry name" value="ARM_TT21_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056833">
        <property type="entry name" value="ARM_TT21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040364">
        <property type="entry name" value="TTC21A/TTC21B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14699">
        <property type="entry name" value="STI2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14699:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25058">
        <property type="entry name" value="ARM_TT21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25060">
        <property type="entry name" value="ARM_TT21_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25068">
        <property type="entry name" value="ARM_TT21_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25064">
        <property type="entry name" value="ARM_TT21_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25063">
        <property type="entry name" value="ARM_TT21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25062">
        <property type="entry name" value="ARM_TT21_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B N-terminal ARM repeat" evidence="7">
        <location>
            <begin position="10"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B second ARM" evidence="6">
        <location>
            <begin position="269"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="324"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="720"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B fourth ARM" evidence="10">
        <location>
            <begin position="756"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="787"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B fifth ARM repeats" evidence="9">
        <location>
            <begin position="950"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="950"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B C-terminal ARM" evidence="8">
        <location>
            <begin position="1101"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1314" mass="150493" checksum="7231A7D29A624269" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5Z9</accession>
    <name>A0A452J5Z9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acidic leucine-rich nuclear phosphoprotein 32 family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Multifunctional protein that is involved in the regulation of many processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the ANP32 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J5Z9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035920"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040661.1">
        <property type="protein sequence ID" value="ENSGAGP00000035920.1"/>
        <property type="gene ID" value="ENSGAGG00000025479.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000040662.1">
        <property type="protein sequence ID" value="ENSGAGP00000035921.1"/>
        <property type="gene ID" value="ENSGAGG00000025479.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000131">
        <property type="entry name" value="acidic leucine-rich nuclear phosphoprotein 32-related protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045081">
        <property type="entry name" value="AN32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11375">
        <property type="entry name" value="ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11375:SF22">
        <property type="entry name" value="ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32 FAMILY MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="101"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="101"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="197"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="213" mass="24339" checksum="E014FACD06DBA572" modified="2019-05-08" version="1">MINVNLLSVSNLPKLNKLRKLELSDNRISGGLEVLAERTPNLTHLNLSGNKIKDINTLEPLKKLPNLHSLDLFNCEVTMLINYRESMFALLPQLTYLDGFDADDQEAPDSDPEADGDGLDDEYDENGEEGEEEDDDEEDELDEEVIDDEEDDEEDDLEGEEEEDGVDDEEEEDEEDEDDDEGEEDLPQGEKRKRDLEDEGEEDPEDEEDEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GEV7</accession>
    <name>A0A452GEV7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RED family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GEV7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000056"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000062.1">
        <property type="protein sequence ID" value="ENSGAGP00000000056.1"/>
        <property type="gene ID" value="ENSGAGG00000000040.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039896">
        <property type="entry name" value="Red-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012492">
        <property type="entry name" value="RED_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012916">
        <property type="entry name" value="RED_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12765">
        <property type="entry name" value="RED PROTEIN IK FACTOR CYTOKINE IK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07807">
        <property type="entry name" value="RED_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07808">
        <property type="entry name" value="RED_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RED-like N-terminal" evidence="7">
        <location>
            <begin position="76"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Protein RED C-terminal" evidence="6">
        <location>
            <begin position="445"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="294"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="332"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="557" mass="65463" checksum="E44FCDB3F316C447" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGK1</accession>
    <name>A0A452GGK1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphodiesterase</fullName>
            <ecNumber evidence="10">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.4.53"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452GGK1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000700"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000789.1">
        <property type="protein sequence ID" value="ENSGAGP00000000700.1"/>
        <property type="gene ID" value="ENSGAGG00000000592.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00762">
        <property type="reaction ID" value="UER00747"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006198">
        <property type="term" value="P:cAMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000001">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040844">
        <property type="entry name" value="PDE4_UCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18100">
        <property type="entry name" value="PDE4_UCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="12">
        <location>
            <begin position="162"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="238"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="473" mass="54154" checksum="57F452C59EC474F5" modified="2019-05-08" version="1">MKEQHSCVGTGHLSMAGYGRMDPLELAVSPVKRLRTEYSFPYLFAEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPKFGVKTEQEDVLAKELEDVNKWGLHVFKIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQTNNRSLHCAFVLTVELTLQPHPYSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIS8</accession>
    <name>A0A452GIS8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Platelet-derived growth factor (PDGF) family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer; disulfide-linked. Also found as heterodimer with PGF.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the PDGF/VEGF growth factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GIS8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001574"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001793.1">
        <property type="protein sequence ID" value="ENSGAGP00000001574.1"/>
        <property type="gene ID" value="ENSGAGG00000001262.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042056">
        <property type="term" value="F:chemoattractant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005172">
        <property type="term" value="F:vascular endothelial growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050930">
        <property type="term" value="P:induction of positive chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045766">
        <property type="term" value="P:positive regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060754">
        <property type="term" value="P:positive regulation of mast cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048010">
        <property type="term" value="P:vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038084">
        <property type="term" value="P:vascular endothelial growth factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00135">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.160.10:FF:000001">
        <property type="entry name" value="Vascular endothelial growth factor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000009">
        <property type="entry name" value="Vascular endothelial growth factor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.160.10">
        <property type="entry name" value="Vascular endothelial growth factor, heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023581">
        <property type="entry name" value="PD_growth_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000072">
        <property type="entry name" value="PDGF/VEGF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050507">
        <property type="entry name" value="PDGF/VEGF_growth_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027928">
        <property type="entry name" value="VEGF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036841">
        <property type="entry name" value="VEGF_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12025">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12025:SF5">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR A, LONG FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00341">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14554">
        <property type="entry name" value="VEGF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00141">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57593">
        <property type="entry name" value="Heparin-binding domain from vascular endothelial growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00249">
        <property type="entry name" value="PDGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50278">
        <property type="entry name" value="PDGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0339">Growth factor</keyword>
    <keyword evidence="8" id="KW-0497">Mitogen</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Platelet-derived growth factor (PDGF) family profile" evidence="13">
        <location>
            <begin position="67"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="160"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="238" mass="27788" checksum="514E41FB7F96092D" modified="2019-05-08" version="1">KKKKKKALNPADQTEPKSGRFQNTVQKAFGKSLPSPLKDNDKERRRQREKLSQAAPTLGDGERKPNEVIKFLEVYERSVCRTIETLVDIFQEYPDEVEYIFKPSCVPLLRCAGCCGDEGLECVPMEVHNVTMEIMRIKPHQSQHIVHMSFLQHSRCDCRPKKDVKAKQEKKSKRGKGKGQKRKRKKSRHCEPCSERRKHLFVQDPQTCKCSCKFTDSRCKSRQLELNERTCRCEKPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLE9</accession>
    <name>A0A452GLE9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sideroflexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GLE9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002444"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002800.1">
        <property type="protein sequence ID" value="ENSGAGP00000002444.1"/>
        <property type="gene ID" value="ENSGAGG00000001977.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015137">
        <property type="term" value="F:citrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF17">
        <property type="entry name" value="SIDEROFLEXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="236" mass="25582" checksum="2EA67437AA43E323" modified="2019-05-08" version="1">MPFRMSGYIPFGTPIVVGLLLPNQTLASTVFWQWLNQSHNACVNYANRNATKPSPTSKFIQGYLGAVISAVSIAVGLNVLVQSANKFTPATRLLIQRFVPFPAVASANICNVVLMRHTELKEGIDVLDSNGNIVGSSRIAAKHALIETALTRVVLPMPILVLPPMIMSIMEKTSLLRSRPRMVLPVQGLVCLAAFGLALPLAISLFPQMSEIETSRLEPEIAMATTSKTVIYNKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNL1</accession>
    <name>A0A452GNL1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP135/TSGA10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GNL1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003325"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003829.1">
        <property type="protein sequence ID" value="ENSGAGP00000003325.1"/>
        <property type="gene ID" value="ENSGAGG00000002683.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22292">
        <property type="entry name" value="cc_Cep135_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051877">
        <property type="entry name" value="Centriole_BasalBody_StrucProt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20544:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN 135KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20544">
        <property type="entry name" value="CENTROSOMAL PROTEIN CEP135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1103"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="68"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="199"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="270"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="451"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="980"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1108"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1128"/>
            <end position="1144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1144" mass="133372" checksum="E6EE03F4E6444013" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPZ8</accession>
    <name>A0A452GPZ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Thyroglobulin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPZ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003906"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004539.1">
        <property type="protein sequence ID" value="ENSGAGP00000003906.1"/>
        <property type="gene ID" value="ENSGAGG00000003196.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035718">
        <property type="term" value="F:macrophage migration inhibitory factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042289">
        <property type="term" value="F:MHC class II protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019882">
        <property type="term" value="P:antigen processing and presentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043518">
        <property type="term" value="P:negative regulation of DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902166">
        <property type="term" value="P:negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001961">
        <property type="term" value="P:positive regulation of cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060907">
        <property type="term" value="P:positive regulation of macrophage cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002830">
        <property type="term" value="P:positive regulation of type 2 immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070206">
        <property type="term" value="P:protein trimerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002286">
        <property type="term" value="P:T cell activation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.870.10">
        <property type="entry name" value="MHC class II-associated invariant chain, trimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043530">
        <property type="entry name" value="CD74_antigen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052001">
        <property type="entry name" value="MHC-II_Gamma/Thyroglobulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015386">
        <property type="entry name" value="MHC_II-assoc_invar/CLIP_MHC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022339">
        <property type="entry name" value="MHC_II-assoc_invar_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011988">
        <property type="entry name" value="MHC_II-assoc_invariant_trimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036613">
        <property type="entry name" value="MHCII_invariant_trimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14093">
        <property type="entry name" value="HLA CLASS II GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14093:SF17">
        <property type="entry name" value="HLA CLASS II HISTOCOMPATIBILITY ANTIGEN GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09307">
        <property type="entry name" value="MHC2-interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08831">
        <property type="entry name" value="MHCassoc_trimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001992">
        <property type="entry name" value="CD74_antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01990">
        <property type="entry name" value="CD74ANTIGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48305">
        <property type="entry name" value="Class II MHC-associated invariant chain ectoplasmic trimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="9">
        <location>
            <begin position="203"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5 6">
        <location>
            <begin position="235"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="313" mass="35027" checksum="AF0CFE328647E0D9" modified="2019-05-08" version="1">MADEDRRGLISTQERPAEDVTAPDSGAQRNYFSRGAVYSAVSILVALLIAGQAVTVYFVYQQSGQISKLTKTSQNLQLEALTRKLPQSAKPTSKLRMAKFSMPMVMRELPLAPKVDMTPLENTAKLNSSTTDQVKHLLLQAAPSKMFPELKNTLMENLNHLKNTMTDRDWQSFESWMYKWLLFEMAKTPQTVQPTEVPADEVQSQCQAESSFSGVYPGRFRPQCDEHGDYLPKQCYASTGYCWCVYKNGTKIEGTETREKLDCPVTPNGLETEELMYSGVDLLKLDSGKGEHLSQPRVGGYDSARGWGELAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTZ5</accession>
    <name>A0A452GTZ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000005519"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006422.1">
        <property type="protein sequence ID" value="ENSGAGP00000005519.1"/>
        <property type="gene ID" value="ENSGAGG00000004476.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="109" mass="12115" checksum="D93CCDD58B778DFF" modified="2019-05-08" version="1">LCLSCKASGFTFSNYQMTWVRQAPGKGLEWVARISSQRGTEQLYSPAVKGRFSISRDNPNNLLYLQXNLLYLQLTGLKPEDTARYRCAGHTVRGSGSELRQKSPGFTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUC3</accession>
    <name>A0A452GUC3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GUC3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005359"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006239.1">
        <property type="protein sequence ID" value="ENSGAGP00000005359.1"/>
        <property type="gene ID" value="ENSGAGG00000004343.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050769">
        <property type="term" value="P:positive regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016698">
        <property type="entry name" value="Numb/numb-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47368">
        <property type="entry name" value="NUMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47368:SF4">
        <property type="entry name" value="NUMB-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="4">
        <location>
            <begin position="39"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="79" mass="8860" checksum="AF1F96FF8E6D09C8" modified="2019-05-08" version="1">MNKLRQSLRRKKPAYVPEASRPHQWQADEEAVRRGKCSFPVRYLGHVEVEESRGMHVCEEAVKKLKTVSAAGTVGGGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUL5</accession>
    <name>A0A452GUL5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GUL5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005779"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006738.1">
        <property type="protein sequence ID" value="ENSGAGP00000005779.1"/>
        <property type="gene ID" value="ENSGAGG00000004702.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF14">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="251"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="7">
        <location>
            <begin position="555"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="213"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="907" mass="103815" checksum="68E61C4F50E2EEE4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUW2</accession>
    <name>A0A452GUW2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Synembryn</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein Ric-8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Chaperone that specifically binds and folds nascent G alpha proteins prior to G protein heterotrimer formation. Also acts as a guanine nucleotide exchange factor (GEF) for G alpha proteins by stimulating exchange of bound GDP for free GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with some GDP-bound G alpha proteins. Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the synembryn family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GUW2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005888"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006868.1">
        <property type="protein sequence ID" value="ENSGAGP00000005888.1"/>
        <property type="gene ID" value="ENSGAGG00000004789.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000447">
        <property type="entry name" value="RIC8 guanine nucleotide exchange factor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008376">
        <property type="entry name" value="Chaperone_Ric-8_A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019318">
        <property type="entry name" value="Gua_nucleotide_exch_fac_Ric8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12425">
        <property type="entry name" value="SYNEMBRYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12425:SF4">
        <property type="entry name" value="SYNEMBRYN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10165">
        <property type="entry name" value="Ric8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01802">
        <property type="entry name" value="SYNEMBRYN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="539" mass="61352" checksum="2765981BBE0FB43E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVN9</accession>
    <name>A0A452GVN9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TNF receptor superfamily member 12A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GVN9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005948"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006935.1">
        <property type="protein sequence ID" value="ENSGAGP00000005948.1"/>
        <property type="gene ID" value="ENSGAGG00000004836.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001238">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022316">
        <property type="entry name" value="TNFR_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32037">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32037:SF2">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12191">
        <property type="entry name" value="stn_TNFRSF12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01962">
        <property type="entry name" value="TNFACTORR12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019389858" description="TNF receptor superfamily member 12A" evidence="2">
        <location>
            <begin position="21"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="11403" checksum="AF6952F209E4CABA" modified="2019-05-08" version="1">METRGKLAMVLLVLLGGAWGEPAAGEGGPGPSCPGGQSWSPDLDKCMDCTICLHRTKNDFCATCGEPQPQDSRLWLAVGGTVGGVAVLGLVGGALLWARCRKREKFTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVR5</accession>
    <name>A0A452GVR5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="4">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452GVR5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006064"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007063.1">
        <property type="protein sequence ID" value="ENSGAGP00000006064.1"/>
        <property type="gene ID" value="ENSGAGG00000004920.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF10">
        <property type="entry name" value="GAMMA-ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="10">
        <location>
            <begin position="117"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="9">
        <location>
            <begin position="223"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="515" mass="56069" checksum="EE2A12F8C4594453" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXI3</accession>
    <name>A0A452GXI3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GXI3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006826"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007922.1">
        <property type="protein sequence ID" value="ENSGAGP00000006826.1"/>
        <property type="gene ID" value="ENSGAGG00000005503.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039938">
        <property type="entry name" value="Sp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14947">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14947:SF24">
        <property type="entry name" value="ZINC FINGER PROTEIN 781-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="327" mass="35725" checksum="08537DF9697B2D2E" modified="2019-05-08" version="1">MPYTCSDAGKALLGTHSLSHIRQSTQERNPTHALSVGKTSIGAQSLSYIRESIQEKSPTHALSVGKTSVRAQALSHIRESTQERRPTHALSVGKSLIGAQSLSNIRESTQERSPTHVPSVVKTSVSTQTLSHIRESTQERSRTHAPSVVKTSISAQTLSDMRESIQERHTTHALSVGKISVGTHTLSNIRESTQERCLTLALSAGKALVGAHTLSHMRESTQERNPTHAPRVVKTSISTHTLSHIIESTQERSPTHAPSVVKTSITTHTLSHIRESTQERNPTHVTSVRKASVIAQALIDIRKSTSERIVINPLTRAGQIFLVFNHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXY6</accession>
    <name>A0A452GXY6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ASPP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXY6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007031"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008147.1">
        <property type="protein sequence ID" value="ENSGAGP00000007031.1"/>
        <property type="gene ID" value="ENSGAGG00000005659.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17225">
        <property type="entry name" value="RA_ASPP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11953">
        <property type="entry name" value="SH3_ASPP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000008">
        <property type="entry name" value="Apoptosis-stimulating of p53 protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000030">
        <property type="entry name" value="Apoptosis-stimulating of p53 protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047163">
        <property type="entry name" value="ASPP1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048942">
        <property type="entry name" value="ASPP2-like_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047166">
        <property type="entry name" value="ASPP2_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24131">
        <property type="entry name" value="APOPTOSIS-STIMULATING OF P53 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24131:SF8">
        <property type="entry name" value="APOPTOSIS-STIMULATING OF P53 PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21801">
        <property type="entry name" value="ASPP2-like_RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01887">
        <property type="entry name" value="SPECTRNALPHA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0728">SH3 domain</keyword>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="990"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="1023"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="1089"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="98"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="421"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="515"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="567"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="756"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="799"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="143"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="436"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="574"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="594"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="799"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1160" mass="129236" checksum="92C29B01CEA3A9CF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZA8</accession>
    <name>A0A452GZA8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLPTM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZA8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007615"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008789.1">
        <property type="protein sequence ID" value="ENSGAGP00000007615.1"/>
        <property type="gene ID" value="ENSGAGG00000006090.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008429">
        <property type="entry name" value="CLPTM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347">
        <property type="entry name" value="CLEFT LIP AND PALATE ASSOCIATED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347:SF14">
        <property type="entry name" value="LIPID SCRAMBLASE CLPTM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05602">
        <property type="entry name" value="CLPTM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="497"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="628"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="664" mass="76253" checksum="754FCD6B8B586C8F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H044</accession>
    <name>A0A452H044_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H044"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007919"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009129.1">
        <property type="protein sequence ID" value="ENSGAGP00000007919.1"/>
        <property type="gene ID" value="ENSGAGG00000006313.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15277">
        <property type="entry name" value="7tmC_RAIG3_GPRC5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051753">
        <property type="entry name" value="RA-inducible_GPCR3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14511">
        <property type="entry name" value="G PROTEIN COUPLED RECEPTOR, CLASS C, GROUP 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14511:SF15">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY C GROUP 5 MEMBER C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019299751" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="9">
        <location>
            <begin position="43"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="429" mass="47495" checksum="4C58078D2C2293D8" modified="2019-05-08" version="1">MATPWFLLMSVWLPLRFLPVCRAQEPPPSGCGTDLPLLYYNLCDLSAAWGIVLEAVASFGAVSSFVLTIILVASLPFVQDPRKKSLVGTQAFFLLGTFGLFCLTFDFIVKRDFSTCTSRRFLFGVLFGICFSCLVAHAITLNFLARRNHGLQGWVTFGVALLLALVEVIINTEWLLITLVRSDGPSQDPCAVANEDFVMALIFVMFLLLAAFAAPWPALCGCYKHWRKHIIFILLTTSLSMTIWVVWIIMYLYGNQQRGGPAWDDPTLAIALVANACVFVVFYIIPEVSHVTRPGPEQTYENDMYPTRGVGYETILKEQKSQSMFVENKAFSMDEPSSAKKPVSPYSGYNGQLVTSVFQPTEMALMHKGPSEGNYDIILPRATANSQAISSANSTLRAEDAYAAQSWHAAAQKDGKGIQAPSPYSKSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1R2</accession>
    <name>A0A452H1R2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATM serine/threonine kinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H1R2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008401"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009673.1">
        <property type="protein sequence ID" value="ENSGAGP00000008401.1"/>
        <property type="gene ID" value="ENSGAGG00000006680.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038980">
        <property type="entry name" value="ATM_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14759" checksum="592D748FC96E4FE3" modified="2019-05-08" version="1">MTLHEPVNTTAGEGVDLSKFTGELDPAPNPPHFPSYVIKATLDYISSCHKTKLKSIVAVLSKKPDSFQKILLAICKQAADTNNIYKKHRILIIYYLFVNLLLKEIKDGLGGAWAFVLRDIIYTLIHYINSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2N7</accession>
    <name>A0A452H2N7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SH3 domain-binding protein 5</fullName>
            <shortName evidence="3">SH3BP-5</shortName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Functions as guanine nucleotide exchange factor (GEF) for RAB11A.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with GDP-bound and nucleotide-free forms of RAB11A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="3">Colocalizes with RAB11A on cytoplasmic vesicle membranes.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">The N-terminal half of the protein mediates interaction with RAB11A and functions as guanine nucleotide exchange factor. Four long alpha-helices (interrupted by a central kink) assemble into coiled coils, giving rise to a 'V' shape.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SH3BP5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H2N7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008886"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010212.1">
        <property type="protein sequence ID" value="ENSGAGP00000008886.1"/>
        <property type="gene ID" value="ENSGAGG00000007029.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007940">
        <property type="entry name" value="SH3BP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19423">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19423:SF1">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05276">
        <property type="entry name" value="SH3BP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="286"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="352"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="160"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="286"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="360"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="373"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="422" mass="47279" checksum="D3951C4B0A00B374" modified="2019-05-08" version="1">MEPAGRRSRPEEGDWPEEEEVDPRIQGELEKLNQSTDDINRRETELEDARQKFRSVLVEATVKLDELVKKIGKAVEDSKPYWEARRVARQAQLEAQKATQDFQRATEVLRAAKETISLAEQRLLEDDKRQFDSAWQEMLNHATQRVMEAEQTKTRSELVHKETAAKYNAAMGRMKQLEKKLKRAINKSKPYFELKAKYYVQLEQFKKTVDDLQAKLALAKGEYKTALKNLEMISDEIHERRRSSAMGPRGCGVGAEGSNTSLEDLSACKLESDSISMASEVFEDDNCSSFVSEEDSETQSVSSFSSGPTSPYEMPTQFPPATRPGSLDLPSPVSLSEFGMVFPVLGPRSECSGASSPECEVERGDRAEGAENKMSNKVNKTRSSSGNSTEGLALDNRMKQLSLQCMEVKEGIIVDRKAVQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2V5</accession>
    <name>A0A452H2V5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H2V5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008848"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010174.1">
        <property type="protein sequence ID" value="ENSGAGP00000008848.1"/>
        <property type="gene ID" value="ENSGAGG00000007000.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12888">
        <property type="entry name" value="SPRY_PRY_TRIM7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="91"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="278" mass="32044" checksum="ACAF2B9D6CC9F2A6" modified="2019-05-08" version="1">MKRQKIVSEFQQLRQFLEEQERLLLAQLEKLDEKIMRIQNENVSKLSKQISHLSELIREMEGKCRKPASEFLQGMFQQSEEISPELEEQVSDFSQKMIVLLETLEKFKVNVTLDPDTAHPELVLSEDRKSVRWKDTWQRLPNNPERFDTEHCVLGCEGFSSGRHCWEVEVGARQYWAVGVARESVGRKGEISHSPEGGIWAVERLDQFRALSSPVTPLPLSRVPSRIRVCLDCDRGQVTFIGAGDEAPIFTFPPGSVPGGRIRPWFWVWRGSRLRLCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3T2</accession>
    <name>A0A452H3T2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMARCC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3T2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009157"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010516.1">
        <property type="protein sequence ID" value="ENSGAGP00000009157.1"/>
        <property type="gene ID" value="ENSGAGG00000007236.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048858">
        <property type="term" value="P:cell projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000014">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049898">
        <property type="entry name" value="MARR_BRCT_CHROMO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032448">
        <property type="entry name" value="SWIRM-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15381:SF1">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15381">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 5 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16498">
        <property type="entry name" value="SWIRM-assoc_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52032">
        <property type="entry name" value="MARR_BRCT_CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="11">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="9">
        <location>
            <begin position="230"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="10">
        <location>
            <begin position="433"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="438"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="62"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="342"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="569"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="604"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="841"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="137"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="360"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="604"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="621"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="656"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="843"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="855"/>
            <end position="865"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="984" mass="108160" checksum="B2824968E2EC198D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H421</accession>
    <name>A0A452H421_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoribosyl pyrophosphate synthase-associated protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Seems to play a negative regulatory role in 5-phosphoribose 1-diphosphate synthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Binds to PRPS1 and PRPS2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H421"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009342"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010733.1">
        <property type="protein sequence ID" value="ENSGAGP00000009342.1"/>
        <property type="gene ID" value="ENSGAGG00000007390.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000012">
        <property type="entry name" value="Phosphoribosyl pyrophosphate synthase-associated protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000014">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF29">
        <property type="entry name" value="PHOSPHORIBOSYL PYROPHOSPHATE SYNTHASE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="8">
        <location>
            <begin position="28"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="369" mass="40713" checksum="4F372756C6A93B07" modified="2019-05-08" version="1">MFCVASTEIGAIMNITKGGLVLFSANSNSSCMELSRRIAERLGVEMGKVQVYQEPNRETRVQIQESVRGKDVFIIQTVSKDVNTTIMELLIMVYACKTSCAKSIIGVIPYFPYSKQCKMRKRGSIVSKLLASMMCKAGLTHLITMDLHQKEIQGFFNIPVDNLRASPFLLQYIQEEIPDYRNAVIVAKSPASAKRAQSFAERLRLGIAVIHGEAQDAESDLVDGRHSPPTAKSVAAIHPSLEIPMLIPKEKPPITVVGDVGGRIAIIVDDIIDDVDSFLAAAETLKERGAYKIFVMATHGLLSSDAPRLIEESAIDEVVVTNTIPHEIQKLQCPKIKTVDISMILSEAIRRIHNGESMSYLFRNIGLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4K6</accession>
    <name>A0A452H4K6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H4K6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009549"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010975.1">
        <property type="protein sequence ID" value="ENSGAGP00000009548.1"/>
        <property type="gene ID" value="ENSGAGG00000007550.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000010977.1">
        <property type="protein sequence ID" value="ENSGAGP00000009549.1"/>
        <property type="gene ID" value="ENSGAGG00000007550.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22117">
        <property type="entry name" value="F-box_FBXL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000050">
        <property type="entry name" value="F-box and leucine-rich repeat protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000628">
        <property type="entry name" value="F-box and leucine-rich repeat protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000152">
        <property type="entry name" value="F-box/LRR-repeat protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF152">
        <property type="entry name" value="F-BOX_LRR-REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="277"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="621" mass="70509" checksum="0333FB554F9201C2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4W5</accession>
    <name>A0A452H4W5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NEK6-subfamily protein kinase</fullName>
            <ecNumber evidence="14">2.7.11.34</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.34" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A452H4W5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009689"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011129.1">
        <property type="protein sequence ID" value="ENSGAGP00000009689.1"/>
        <property type="gene ID" value="ENSGAGG00000007649.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030071">
        <property type="term" value="P:regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08224">
        <property type="entry name" value="STKc_Nek6_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000148">
        <property type="entry name" value="Serine/threonine-protein kinase Nek7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000204">
        <property type="entry name" value="Serine/threonine-protein kinase Nek7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000240">
        <property type="entry name" value="Serine/threonine-protein kinase Nek7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43289">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43289:SF13">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="47"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="315" mass="36484" checksum="9785D3ACA6A2DDE6" modified="2019-05-08" version="1">MEGQHNHPHHLGDQNNPLCKVPGPEYQHDPQQRHLTSYPYRCTMADFQIEKKIGRGQFSEVYKATCLLDRKTVALKKVQIFEMMDAKARQDCIKEIDLLKQLNHPNIIKYLDSFIEDNELNIVLELADAGDLSQMIKYFKKQKRLIPERTVWKYFVQLCSAVEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLFSLCQKIEQCDYPPLPAEHYSEKLRELVSMCIYPDPDQRPDVGYVHQIAKQLHVWTSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7I7</accession>
    <name>A0A452H7I7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GPX4 peroxidase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H7I7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010710"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012280.1">
        <property type="protein sequence ID" value="ENSGAGP00000010710.1"/>
        <property type="gene ID" value="ENSGAGG00000008354.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="8424" checksum="62885F395D080C5C" modified="2019-05-08" version="1">RCRQGQPEGSLCTASSQVPAPLLPLAGPRIRSGCAQADDWRSAKSVYEFHARDIDGRDVSLEKYRGYVCIITNVASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8Q7</accession>
    <name>A0A452H8Q7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">poly(A)-specific ribonuclease</fullName>
            <ecNumber evidence="6">3.1.13.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452H8Q7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011125"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012740.1">
        <property type="protein sequence ID" value="ENSGAGP00000011125.1"/>
        <property type="gene ID" value="ENSGAGG00000008605.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000002">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 6 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000008">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 6 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF35">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="18" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="19" id="KW-0804">Transcription</keyword>
    <keyword evidence="17" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="16" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="21">
        <location>
            <begin position="188"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="517" mass="58922" checksum="7BE7175B352DF30D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8W5</accession>
    <name>A0A452H8W5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Fibrillin-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibrillin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000011184"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012807.1">
        <property type="protein sequence ID" value="ENSGAGP00000011184.1"/>
        <property type="gene ID" value="ENSGAGG00000008649.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000012822.1">
        <property type="protein sequence ID" value="ENSGAGP00000011195.1"/>
        <property type="gene ID" value="ENSGAGG00000008649.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000012837.1">
        <property type="protein sequence ID" value="ENSGAGP00000011210.1"/>
        <property type="gene ID" value="ENSGAGG00000008649.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001527">
        <property type="term" value="C:microfibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000005">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000023">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000044">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000049">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000058">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000071">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000086">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000087">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000097">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000107">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000141">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000159">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000196">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000223">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000005">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000006">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000007">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000009">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000010">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000011">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000133">
        <property type="entry name" value="Fibrillin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000003">
        <property type="entry name" value="Fibrillin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000008">
        <property type="entry name" value="Fibrillin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:001015">
        <property type="entry name" value="Fibrillin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000020">
        <property type="entry name" value="Fibrillin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000003">
        <property type="entry name" value="fibrillin-1 isoform X1"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000002">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000010">
        <property type="entry name" value="Pro-epidermal growth factor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000096">
        <property type="entry name" value="Putative fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000008">
        <property type="entry name" value="Signal peptide, CUB domain, EGF-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="46"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049388">
        <property type="entry name" value="FBN_EGF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040872">
        <property type="entry name" value="Fibrillin_U_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052080">
        <property type="entry name" value="vWF_C/EGF_Fibrillin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333:SF5">
        <property type="entry name" value="FIBRILLIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333">
        <property type="entry name" value="VON WILLEBRAND FACTOR C AND EGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21364">
        <property type="entry name" value="EGF_FBN_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18193">
        <property type="entry name" value="Fibrillin_U_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00683">
        <property type="entry name" value="TB"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036312">
        <property type="entry name" value="Fibrillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="47"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="44"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="42"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="40"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0372">Hormone</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604493" description="Fibrillin-1" evidence="16">
        <location>
            <begin position="19"/>
            <end position="2872"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="150"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="18">
        <location>
            <begin position="188"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="250"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="292"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="18">
        <location>
            <begin position="338"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="449"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="490"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="530"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="572"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="613"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="18">
        <location>
            <begin position="659"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="723"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="765"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="807"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="18">
        <location>
            <begin position="851"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="910"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="18">
        <location>
            <begin position="956"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1028"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1070"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1113"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1155"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1238"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1280"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1322"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1363"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1404"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1446"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1487"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="18">
        <location>
            <begin position="1532"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1606"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1648"/>
            <end position="1684"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="18">
        <location>
            <begin position="1693"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1766"/>
            <end position="1807"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1808"/>
            <end position="1848"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1849"/>
            <end position="1890"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1891"/>
            <end position="1926"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1930"/>
            <end position="1972"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1973"/>
            <end position="2012"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="2013"/>
            <end position="2054"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="18">
        <location>
            <begin position="2059"/>
            <end position="2111"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="2127"/>
            <end position="2163"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="2166"/>
            <end position="2205"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="2291"/>
            <end position="2328"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="18">
        <location>
            <begin position="2337"/>
            <end position="2390"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="2402"/>
            <end position="2443"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="2485"/>
            <end position="2521"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="2524"/>
            <end position="2566"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="2567"/>
            <end position="2606"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="2607"/>
            <end position="2643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2727"/>
            <end position="2749"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="494"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="811"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="1895"/>
            <end position="1905"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="2571"/>
            <end position="2581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2872" mass="312769" checksum="F3A461FEAA282915" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9B5</accession>
    <name>A0A452H9B5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan 2,3-dioxygenase</fullName>
            <shortName evidence="1">TDO</shortName>
            <ecNumber evidence="1">1.13.11.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tryptamin 2,3-dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Tryptophan oxygenase</fullName>
            <shortName evidence="1">TO</shortName>
            <shortName evidence="1">TRPO</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Tryptophan pyrrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Tryptophanase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">TDO2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Heme-dependent dioxygenase that catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring and converts L-tryptophan to N-formyl-L-kynurenine. Catalyzes the oxidative cleavage of the indole moiety.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-tryptophan + O2 = N-formyl-L-kynurenine</text>
            <dbReference type="Rhea" id="RHEA:24536"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58629"/>
            <dbReference type="EC" id="1.13.11.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="1">Binds 1 heme group per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer. Dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tryptophan 2,3-dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.11" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A452H9B5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011356"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013001.1">
        <property type="protein sequence ID" value="ENSGAGP00000011356.1"/>
        <property type="gene ID" value="ENSGAGG00000008779.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00333">
        <property type="reaction ID" value="UER00453"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004833">
        <property type="term" value="F:L-tryptophan 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019442">
        <property type="term" value="P:L-tryptophan catabolic process to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01972">
        <property type="entry name" value="T23O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037217">
        <property type="entry name" value="Trp/Indoleamine_2_3_dOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004981">
        <property type="entry name" value="Trp_2_3_dOase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10138">
        <property type="entry name" value="TRYPTOPHAN 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10138:SF0">
        <property type="entry name" value="TRYPTOPHAN 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03301">
        <property type="entry name" value="Trp_dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140959">
        <property type="entry name" value="Indolic compounds 2,3-dioxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0823">Tryptophan catabolism</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="212"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="379" mass="44434" checksum="1875AD4E8F4B71D9" modified="2025-10-08" version="2">MEDEEENKSQEGLNKASKGGLIYGDYLHLDKILNAQELQSEKKGNKIHDEHLFIVTHQAYELWFKQILWELDSVRVIFQNGRVRDERNMLKVVARMHRISMILKLLVEQFSILETMTALDFFDFRDYLSPASGFQSLQFRLLENKIGVPQSLRVPYNRRHYRDNFKGHENELLLKSEQEPTLLQLVEAWLERTPGLEPEGFNFWGKLEVNVLKSLEKELAMVQAKQESEEKEDLLAELQKQKEVLISLFDEKRHEHLLSKGERRLSYKALKGALMIYFYRYNKAIFLQDLVGMLTKGRNNHVCMVHRMIGSKAGTGGSSGYQYLRSTVSDRYKVFVDLFNLSTFLVPRHWIPKMNPTIHKFLYTAEYCDSSYFSSDDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9N0</accession>
    <name>A0A452H9N0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H9N0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011526"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013198.1">
        <property type="protein sequence ID" value="ENSGAGP00000011526.1"/>
        <property type="gene ID" value="ENSGAGG00000008899.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF42">
        <property type="entry name" value="VOLTAGE-GATED HYDROGEN CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="3">
        <location>
            <begin position="17"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="3">
        <location>
            <begin position="56"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="174" mass="20797" checksum="94DDA10E983E1741" modified="2019-05-08" version="1">MYLSVSLSSSSSATLVSRIIFYEDRDFQGRSYAVTADQPDMHAHLNRCNSIQVESGCWMIYERPHYIGHQYFLKKGEYPNYQQWMGFNDSVKSCLAHIPLCRWRQRRIRVYERNDVGGRMTESFCDYPSLCDEFHPRDIQSCHVFEGNWIFCGWPNYRAWQYLLRAREYWGVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HA89</accession>
    <name>A0A452HA89_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane channel-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TMC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HA89"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011637"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013332.1">
        <property type="protein sequence ID" value="ENSGAGP00000011637.1"/>
        <property type="gene ID" value="ENSGAGG00000008986.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012496">
        <property type="entry name" value="TMC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF45">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07810">
        <property type="entry name" value="TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="547"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="580"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="663"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="707"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="753"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="803"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="domain" description="TMC" evidence="8">
        <location>
            <begin position="794"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU310713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="826" mass="92981" checksum="D6AD25E707936656" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAD9</accession>
    <name>A0A452HAD9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HAD9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011686"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013388.1">
        <property type="protein sequence ID" value="ENSGAGP00000011686.1"/>
        <property type="gene ID" value="ENSGAGG00000009028.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001378">
        <property type="entry name" value="Immunoglobulin kappa variable 4-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="121"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="226" mass="24211" checksum="3DFEBCE590F5EA1E" modified="2019-05-08" version="1">GESPAENTSGQIVMTQTPESLAVSPGEKVTINCKASSSLTYEGKEYLAWYQQKTGQAPKLLIYYASTHASGLPDRFSGSGSGTDFTLTISRVEAEDSGDYYCLQSYGTPLTVIQTSTKTTLRCSVQGNSCFLCFPPVSQSITPTAVSQSRDLPVRQAPKQLIYLASTLQSGVPARFSGSGSGTDFTLTISKVEAEDAGDYYCQQSRSDPLTVIQTNTKTSILFRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAL2</accession>
    <name>A0A452HAL2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BOS complex subunit NCLN</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nicastrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HAL2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011751"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013462.1">
        <property type="protein sequence ID" value="ENSGAGP00000011751.1"/>
        <property type="gene ID" value="ENSGAGG00000009044.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03882">
        <property type="entry name" value="M28_nicalin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000021">
        <property type="entry name" value="Nicalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016574">
        <property type="entry name" value="Nicalin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31826">
        <property type="entry name" value="NICALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019013127" description="BOS complex subunit NCLN" evidence="10">
        <location>
            <begin position="42"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="11">
        <location>
            <begin position="216"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="441" mass="48923" checksum="96A980F14C420E06" modified="2019-05-08" version="1">MLEEAGEVLESVLKASCLPLSFLLFVPAVLLLLGPPPAAEAAHEFTVYRMQQYELGGQPYGTRNAVLNTEARTLEADVLSRRCVMMRLVDFSYEQYQKALRQSAGAVVIILPKAMSALPQDVVRQFMEIEPEMLAMETIVPVYFAVEDEELLSIYEQTRVASTSQGSASAAEVLLHTATANGFQMVTSGAQSKAVSDWLITSLEGRLTGLGGEDLPTIVIVAHYDSFGVAPWLSHGADSNGSGISGLLELARLFSRLYTYKRTHAGYNLLFFASGGGKFNYQGTKRWLEDNLDHTDSSLLQDNVAFVLCLDTLGRGNSLHLHVSKPPKEGTLQHAFLRELEMVVASQFPEVKFSMVHKKINLAEDMLAWEHERFAIRRLPAFTISHLESHRDGLRNSIMDLRSRVDSKTLTRNTRIIAEALTRVIYNLTEKGAPADLQIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAM7</accession>
    <name>A0A452HAM7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">OCEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ELL/occludin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HAM7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011788"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013500.1">
        <property type="protein sequence ID" value="ENSGAGP00000011788.1"/>
        <property type="gene ID" value="ENSGAGG00000009086.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2670:FF:000002">
        <property type="entry name" value="RNA polymerase II elongation factor ELL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019464">
        <property type="entry name" value="ELL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF8">
        <property type="entry name" value="RNA POLYMERASE II ELONGATION FACTOR ELL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10390">
        <property type="entry name" value="ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07303">
        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144292">
        <property type="entry name" value="occludin/ELL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OCEL" evidence="9">
        <location>
            <begin position="524"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="291"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="354"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="454"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="300"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="359"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="455"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="469"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="657" mass="74005" checksum="89206DB38B7A6DE7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDK8</accession>
    <name>A0A452HDK8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutathione hydrolase</fullName>
            <ecNumber evidence="3">2.3.2.2</ecNumber>
            <ecNumber evidence="3">3.4.19.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Gamma-glutamyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Gamma-glutamyltranspeptidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Cleaves the gamma-glutamyl peptide bond of glutathione and glutathione conjugates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>an N-terminal (5-L-glutamyl)-[peptide] + an alpha-amino acid = 5-L-glutamyl amino acid + an N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:28807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; glutathione metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.2" evidence="3"/>
    <dbReference type="EC" id="3.4.19.13" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452HDK8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012889"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014772.1">
        <property type="protein sequence ID" value="ENSGAGP00000012889.1"/>
        <property type="gene ID" value="ENSGAGG00000009892.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.40:FF:000002">
        <property type="entry name" value="gamma-glutamyltransferase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF54">
        <property type="entry name" value="GLUTATHIONE HYDROLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="141"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="577"/>
            <end position="579"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="600"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="652"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU368068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="748" mass="80122" checksum="ED0A5CE88BA05211" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDM2</accession>
    <name>A0A452HDM2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HDM2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012906"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014792.1">
        <property type="protein sequence ID" value="ENSGAGP00000012906.1"/>
        <property type="gene ID" value="ENSGAGG00000009905.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000014794.1">
        <property type="protein sequence ID" value="ENSGAGP00000012908.1"/>
        <property type="gene ID" value="ENSGAGG00000009905.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000004">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily B, member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF8">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="109"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="52"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="374" mass="42432" checksum="201D92C66CEE4F1B" modified="2019-05-08" version="1">MESNRDEAERCIAIAVAAIKASQPDKALRFLEKAQRLYPSQRVRVLIESLTKNEQSANGQSKSRESTNAHFRKMSGDFPSANGEAGGETSKGYTQDQVDAVKRVKQCKDYYEILGVNREASDEDLKKAYRKLALKFHPDKNHAPGATEAFKAIGNAYAVLSNPEKRTQYDQFGDEKVNPSRHGHSHTDFHRGFEADISPEDLFNMFFGGGFPSSNVHVYSNGRMRYTYHQRHDRREHQGDGGLGLFVQLMPILILIIVSALSQMMVSSPPYSLSHRPSVGHIHRRVTEHLKVAYYVAENFAEEYTGTNLKNVERSVEDDYIANLRNNCWKEKQQKEGLLYRARYFGDSDLYQRAQRMGTPSCSRLSEVQASMHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEU5</accession>
    <name>A0A452HEU5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000013344"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015272.1">
        <property type="protein sequence ID" value="ENSGAGP00000013344.1"/>
        <property type="gene ID" value="ENSGAGG00000010202.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071391">
        <property type="term" value="P:cellular response to estrogen stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032355">
        <property type="term" value="P:response to estradiol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.20:FF:000002">
        <property type="entry name" value="Vitellogenin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.230.10:FF:000002">
        <property type="entry name" value="Vitellogenin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.230.10">
        <property type="entry name" value="Lipovitellin, beta-sheet shell regions, chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.80.10">
        <property type="entry name" value="Lipovitellin-phosvitin complex, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.50.20">
        <property type="entry name" value="Lipovitellin. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.90.10">
        <property type="entry name" value="Vitellinogen, beta-sheet shell domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.20">
        <property type="entry name" value="Vitellinogen, superhelical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015819">
        <property type="entry name" value="Lipid_transp_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011030">
        <property type="entry name" value="Lipovitellin_superhlx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015816">
        <property type="entry name" value="Vitellinogen_b-sht_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015258">
        <property type="entry name" value="Vitellinogen_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037088">
        <property type="entry name" value="Vitellinogen_b-sht_shell_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015255">
        <property type="entry name" value="Vitellinogen_open_b-sht"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015817">
        <property type="entry name" value="Vitellinogen_open_b-sht_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050733">
        <property type="entry name" value="Vitellogenin/Apolipophorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001747">
        <property type="entry name" value="Vitellogenin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345:SF15">
        <property type="entry name" value="VITELLOGENIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345">
        <property type="entry name" value="VITELLOGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09175">
        <property type="entry name" value="Vit_b-sht_shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09172">
        <property type="entry name" value="Vit_open_b-sht"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01347">
        <property type="entry name" value="Vitellogenin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01169">
        <property type="entry name" value="DUF1943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01170">
        <property type="entry name" value="DUF1944"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00638">
        <property type="entry name" value="LPD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56968">
        <property type="entry name" value="Lipovitellin-phosvitin complex, beta-sheet shell regions"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48431">
        <property type="entry name" value="Lipovitellin-phosvitin complex, superhelical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51211">
        <property type="entry name" value="VITELLOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0758">Storage protein</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019379760" evidence="8">
        <location>
            <begin position="21"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="domain" description="Vitellogenin" evidence="9">
        <location>
            <begin position="17"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="10">
        <location>
            <begin position="1476"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1091"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1115"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1139"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1154"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="155"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="197"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1748" mass="193793" checksum="F3AC82F2CB59EBC5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFD9</accession>
    <name>A0A452HFD9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mRNA(cytosine(6666)) deaminase</fullName>
            <ecNumber evidence="4">3.5.4.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">mRNA(cytosine(6666)) deaminase 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Probable C to U editing enzyme whose physiological substrate is not yet known. Does not display detectable apoB mRNA editing. Has a low intrinsic cytidine deaminase activity. May play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>cytidine(6666) in apoB mRNA + H2O + H(+) = uridine(6666) in apoB mRNA + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21772"/>
            <dbReference type="Rhea" id="RHEA-COMP:13888"/>
            <dbReference type="Rhea" id="RHEA-COMP:13889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="3.5.4.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.36" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452HFD9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013543"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015504.1">
        <property type="protein sequence ID" value="ENSGAGP00000013543.1"/>
        <property type="gene ID" value="ENSGAGG00000010347.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016554">
        <property type="term" value="P:cytidine to uridine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01283">
        <property type="entry name" value="cytidine_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000031">
        <property type="entry name" value="Probable C-&gt;U-editing enzyme APOBEC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050610">
        <property type="entry name" value="APOBEC_Cyt_Deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13857:SF4">
        <property type="entry name" value="C-U-EDITING ENZYME APOBEC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13857">
        <property type="entry name" value="MRNA EDITING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18772">
        <property type="entry name" value="APOBEC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="13">
        <location>
            <begin position="46"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="221" mass="25837" checksum="CA8FDCFF65F13654" modified="2019-05-08" version="1">MAENQEKPSDTQTGEEQPENAEESEEKKKKELEKLPPFEIVGGERFPAFFFKFQFRNVEYSSGRNKTFLCYVVETQGKESGSFRGYLEDEHAAAHVEDAFFNNILPTCDPNLRYNVTWYVSSSPCVPCAGRIAQILQKNKNLRLSILVSRLFMWEEPEMQAALKKLKTSGCKLKIMKPQDFEYIWQNFVEQEEGEAKAFEPWEDIQENFLYYEEKLSEVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFU1</accession>
    <name>A0A452HFU1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Retinoic acid receptor RXR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nuclear receptor subfamily 2 group B member</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for retinoic acid that acts as a transcription factor. Forms homo- or heterodimers with retinoic acid receptors (rars) and binds to target response elements in response to their ligands, all-trans or 9-cis retinoic acid, to regulate gene expression in various biological processes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Heterodimer; with a rar molecule.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFU1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013740"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015734.1">
        <property type="protein sequence ID" value="ENSGAGP00000013740.1"/>
        <property type="gene ID" value="ENSGAGG00000010465.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003707">
        <property type="term" value="F:nuclear steroid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06956">
        <property type="entry name" value="NR_DBD_RXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000005">
        <property type="entry name" value="Retinoic acid receptor RXR-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021780">
        <property type="entry name" value="Nuc_recep-AF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000003">
        <property type="entry name" value="Retinoid-X_rcpt/HNF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11825">
        <property type="entry name" value="Nuc_recep-AF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00545">
        <property type="entry name" value="RETINOIDXR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="150"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="244"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="224"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="399" mass="42988" checksum="50F0EC29C55A1298" modified="2019-05-08" version="1">ARRRRWRSLDQAGARGGLRPGSPRQGAGTRGMGDRGRDCRSPDSSSLSSSPPPELSAPLHPSMISSAMTSSINSPISSLGSPFPVISSSLGSPGLPATPSSGYGPVSSPQINSTVNLSGMHSVSSSEDVKPPLGVRSLPCHPHGGMMPGKRLCAICGDRSSGKHYGVYSCEGCKGFFKRTIRKDLTYTCRDNKDCIVDKRQRNRCQYCRYQKCLATGMKREAVQEERQRGKEKDGEGDFAGGANEEMPVEKILEAELAVEQKSDQSVDGSGTGGSSPNDPVTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRSISVKDGILLATGLHVHRNSAHSAGVGAIFDRVLTELVSKMRDMRMDKTELGCLRAIILFNPGKGPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGY0</accession>
    <name>A0A452HGY0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HGY0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014133"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016181.1">
        <property type="protein sequence ID" value="ENSGAGP00000014133.1"/>
        <property type="gene ID" value="ENSGAGG00000010769.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051018">
        <property type="term" value="F:protein kinase A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12264">
        <property type="entry name" value="RRM_AKAP17A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056852">
        <property type="entry name" value="AK17A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12484:SF2">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 17A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12484">
        <property type="entry name" value="B-LYMPHOCYTE ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25015">
        <property type="entry name" value="RBD_AKAP-17A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="403" mass="47184" checksum="446F39E550FE46A7" modified="2019-05-08" version="1">MAAATIVHDASEAVELCASYGLYLKPITKMSISVALPQLKQPGKSISNWEVMERLKGMVQTHQFSTLRISKSTMDFIRFEGEVENKSLVKSFLACLDGKTIKLSGFSDILKVRAAEYKIDFPTRHDWDSFFRDAKDMNETLPGERPDTIHLEGLPCKWFALKDSGSEKPSEEVLIKVFKKFGEIRNVDIPMLDPYREEMTGRNFHTFSFGGHLNFEAYVQYHEYAGFIKAMNALRGMKLMYKGDDGKAVACNVKVSFDSTKHLSDASIKKRQLERQKLQELEQQREEQKRREKEAEEKKKEEERKQKELEELERERKREEKLRKREQKQKDREARRNKKKLEKMQAEEQKKLQEKIRLEERKLLLAQRNLQSIRLIAELLSRAKVTLESCLFRIISRANKNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HH49</accession>
    <name>A0A452HH49_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Metalloendopeptidase</fullName>
            <ecNumber evidence="15">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14 15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">Secretory vesicle</location>
            <location evidence="12">Cortical granule</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A452HH49"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014201"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016251.1">
        <property type="protein sequence ID" value="ENSGAGP00000014201.1"/>
        <property type="gene ID" value="ENSGAGG00000010811.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060473">
        <property type="term" value="C:cortical granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd04283">
        <property type="entry name" value="ZnMc_hatching_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000013">
        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000040">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017370">
        <property type="entry name" value="Hatching_enzyme_Uvs2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034039">
        <property type="entry name" value="ZnMP_hatching_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF899">
        <property type="entry name" value="ASTACIN-LIKE METALLOENDOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038057">
        <property type="entry name" value="Hatching_enzyme_Uvs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0278">Fertilization</keyword>
    <keyword evidence="6 14 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14 15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 14 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <keyword evidence="7 14 15" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018808581" description="Metalloendopeptidase" evidence="15">
        <location>
            <begin position="20"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="17">
        <location>
            <begin position="49"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="16">
        <location>
            <begin position="249"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="16">
        <location>
            <begin position="355"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="470" mass="52540" checksum="ABB8E9D09A07C671" modified="2019-05-08" version="1">MNLIPLSALLAFFYSFALCKPVQVATRNNEMDSETTLHESDIVLRRKRNAINCDSSCFWPKSRDGLVKVPVNVSTEFSVEERTWIVEAMQEFTTLTCVQFINRTTETNYISIVPGESCWSHFGKIGGMQQVGLMKTGCMSKGAIQHEMNHALGFLHEQARSDRDNYVKIMWQYITAGEQGNFGKVNSNNLGLPYDYSSVMHYGLYDFSNTFGKATIVPIPDPSVPIGQREGLSNLDVAKINKLYSCNCCSNVLHKPTDTFSSANYPSSYPDNSHCLWLIRIPQHQVFLQFDAFDLQPSSDCTSDYIRIYDGSSRNSSVLLDKLCGMGSLPSLVASRNVMLVEFVSDEATAATVKCGSTLMGTRGVITSPNYPNRYPKNQDCFWIISAPADLKISLTMVSFELEDTDECKYDYLLIHDGSRPTSPAVGPYCGTMQIADFTSSENIVLVEFHSDVAWVFPGFMLNYTFVMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJ22</accession>
    <name>A0A452HJ22_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Carnitine O-palmitoyltransferase 1, muscle isoform</fullName>
            <ecNumber evidence="4">2.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Carnitine O-palmitoyltransferase I, muscle isoform</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Carnitine palmitoyltransferase 1B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(R)-carnitine + hexadecanoyl-CoA = O-hexadecanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:12661"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:17490"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="EC" id="2.3.1.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:12662"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.21" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452HJ22"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014877"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016995.1">
        <property type="protein sequence ID" value="ENSGAGP00000014877.1"/>
        <property type="gene ID" value="ENSGAGG00000011258.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000016998.1">
        <property type="protein sequence ID" value="ENSGAGP00000014879.1"/>
        <property type="gene ID" value="ENSGAGG00000011258.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004095">
        <property type="term" value="F:carnitine O-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015909">
        <property type="term" value="P:long-chain fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000001">
        <property type="entry name" value="Carnitine O-palmitoyltransferase 1, liver isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000042">
        <property type="entry name" value="Carnitine Palmitoyl Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032476">
        <property type="entry name" value="CPT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF69">
        <property type="entry name" value="CARNITINE O-PALMITOYLTRANSFERASE 1, MUSCLE ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16484">
        <property type="entry name" value="CPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carnitine O-palmitoyltransferase N-terminal" evidence="23">
        <location>
            <begin position="17"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="22">
        <location>
            <begin position="190"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF16484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="774" mass="88269" checksum="A30DF4F001C449B0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HK33</accession>
    <name>A0A452HK33_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Bleomycin hydrolase</fullName>
            <ecNumber evidence="3 9">3.4.22.40</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.</text>
            <dbReference type="EC" id="3.4.22.40"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.40" evidence="3 9"/>
    <dbReference type="AlphaFoldDB" id="A0A452HK33"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015280"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017457.1">
        <property type="protein sequence ID" value="ENSGAGP00000015280.1"/>
        <property type="gene ID" value="ENSGAGG00000011528.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070005">
        <property type="term" value="F:cysteine-type aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043418">
        <property type="term" value="P:homocysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00585">
        <property type="entry name" value="Peptidase_C1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000021">
        <property type="entry name" value="Bleomycin hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004134">
        <property type="entry name" value="Peptidase_C1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363:SF2">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03051">
        <property type="entry name" value="Peptidase_C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005700">
        <property type="entry name" value="PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005700-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="455" mass="52285" checksum="C147D24ED2E51A64" modified="2019-05-08" version="1">MDAPGLSLEKIAAFTKKLASEPQFLLARNVATTSDPLEVCLDRQVVQETVHVFQHTVPVEGKPVTNQKSSGRCWIFSCLNVMRLPVMKKFNIEEFEFSQAYIYYWDKIERCYYFLNAFMETAQKNKPVEDRLTQFLLSNPSNDGGQWDMLVNIIEKHGVIPKKNFPESHTTEASRRMNEILNHKMREYCVRLRNMVASGSTKEELTAAKDSMIEEVFRIVSTCLGNPPETFSWEFRDKEKNYHKIGPVTPLQFYQEHIKPLFSMEDKICLVNDPRPQNHYNKLYTVAYLGNLVGGRKTLYNNQPVDLLKKLAAASIKGGEAVWFGCDVGKYFHSKLGIIDLNVFDHELVFGVSVKNMNKAERLIFGESMMTHAMVLTAFTEKDGQEGSFEKWKVENSWGEDRGIKGYLIMSDDWFSEYVYEVVVDKKYVPEDILAVMQQEPIVLPAWDPMGALAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HL29</accession>
    <name>A0A452HL29_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C-C motif chemokine</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the intercrine beta (chemokine CC) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HL29"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015626"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017846.1">
        <property type="protein sequence ID" value="ENSGAGP00000015626.1"/>
        <property type="gene ID" value="ENSGAGG00000011773.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048020">
        <property type="term" value="F:CCR chemokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061844">
        <property type="term" value="P:antimicrobial humoral immune response mediated by antimicrobial peptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070098">
        <property type="term" value="P:chemokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048245">
        <property type="term" value="P:eosinophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00272">
        <property type="entry name" value="Chemokine_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000002">
        <property type="entry name" value="C-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000827">
        <property type="entry name" value="Chemokine_CC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF103">
        <property type="entry name" value="C-C MOTIF CHEMOKINE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00472">
        <property type="entry name" value="SMALL_CYTOKINES_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="3 6" id="KW-0202">Cytokine</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018809420" description="C-C motif chemokine" evidence="6">
        <location>
            <begin position="23"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="7">
        <location>
            <begin position="30"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="91" mass="9949" checksum="A68054E02DBE8F61" modified="2019-05-08" version="1">MKVSVAALAVLLIAAFCSLASSAPIGSDPPTACCFTYTSRKIPRGLVVDYYDTNSMCSQTAIVFITKKGREVCANPKEDWVQEYRPNQEPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLF5</accession>
    <name>A0A452HLF5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">MHC class I-like antigen recognition-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HLF5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015791"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018027.1">
        <property type="protein sequence ID" value="ENSGAGP00000015791.1"/>
        <property type="gene ID" value="ENSGAGG00000011875.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042612">
        <property type="term" value="C:MHC class I protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002474">
        <property type="term" value="P:antigen processing and presentation of peptide antigen via MHC class I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000001">
        <property type="entry name" value="H-2 class I histocompatibility antigen, alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF242">
        <property type="entry name" value="MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I-RELATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0490">MHC I</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="MHC class I-like antigen recognition-like" evidence="11">
        <location>
            <begin position="31"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="233" mass="27136" checksum="2725770821E45065" modified="2019-05-08" version="1">LRDTFPGFSLGSLAGSLSLIHISPPTPPGFHSRRVLDTVVSEPGPGLPWYSRVVYVDDQVIYVYTSGMQRAEPRPVWMAQNEGPEFWDERTWWAQRHQKWFKASLNTLGQLYNQSKGFHIIQMKYGCDLREDNTTWGFYQDSYDGRDFLTFDTESMTLGAADIGAQITKRRWDAEVFDNQQWKRYLEERCIPWLRSSLEYGKETLQRKGKMGDKPYPEGHYKLYLHSPAPVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLK9</accession>
    <name>A0A452HLK9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HLK9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015871"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018134.1">
        <property type="protein sequence ID" value="ENSGAGP00000015871.1"/>
        <property type="gene ID" value="ENSGAGG00000011941.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46829">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46829:SF1">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="76" mass="8626" checksum="EBF0A83D08FE48AF" modified="2019-05-08" version="1">MGITDFDHMKEISWHVRELLGVEEPLWSRSIALRYRDNLGLFLEHKAPTGAKADALTFSQFVQEAGLEPYATIPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HM03</accession>
    <name>A0A452HM03_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HM03"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016012"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018292.1">
        <property type="protein sequence ID" value="ENSGAGP00000016012.1"/>
        <property type="gene ID" value="ENSGAGG00000012041.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01345">
        <property type="entry name" value="CERVTRCPTASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="313" mass="36396" checksum="3E409A5D9CC2BB57" modified="2019-05-08" version="1">MGMNMKVVRWIRNWLKGRLQRVVLKGELSGWKEVTSGVPQGSVLGPILFNLFITDLGTKSGNVLIKFADDTKLGGIANTEKDRDTIQEDLNHLVNWSNRNRMKYNSEKCKVMHLGINNKNFGYTLGAHQLEATEEEKDLGVLVDSRMTMSRQCDTAVKKANAILGCIRRGISCKDKEVLVPLYKALVRPHLEYCVQFWCPMFKKDEFKLEQVQRRATRMIRGMENLPYERRLKELGLFSLAKRRLRGDMLALYKYIRGVNVREGEELFKFSTNVGTRTNGYKLDIRKFRLEIRRRFLTIRGVKFWNSLPREVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HP85</accession>
    <name>A0A452HP85_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPATS2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HP85"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016784"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019154.1">
        <property type="protein sequence ID" value="ENSGAGP00000016780.1"/>
        <property type="gene ID" value="ENSGAGG00000012537.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000019158.1">
        <property type="protein sequence ID" value="ENSGAGP00000016784.1"/>
        <property type="gene ID" value="ENSGAGG00000012537.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009816">
        <property type="entry name" value="SPATS2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15623:SF8">
        <property type="entry name" value="SPATS2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15623">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED SERINE-RICH PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07139">
        <property type="entry name" value="SPATS2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="378"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="395"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="467"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="555" mass="62246" checksum="A6FEDDD1711FD32E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HPY1</accession>
    <name>A0A452HPY1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Neural-cadherin-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cell membrane</location>
            <topology evidence="1 15">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000017069"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019480.1">
        <property type="protein sequence ID" value="ENSGAGP00000017069.1"/>
        <property type="gene ID" value="ENSGAGG00000012743.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016327">
        <property type="term" value="C:apicolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035332">
        <property type="term" value="P:positive regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090251">
        <property type="term" value="P:protein localization involved in establishment of planar polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000007">
        <property type="entry name" value="Cadherin 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000020">
        <property type="entry name" value="Dachsous cadherin-related 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000033">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000024">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000562">
        <property type="entry name" value="Neural-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000125">
        <property type="entry name" value="Neural-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000136">
        <property type="entry name" value="Neural-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000157">
        <property type="entry name" value="Neural-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000204">
        <property type="entry name" value="Neural-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000040">
        <property type="entry name" value="neural-cadherin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000119">
        <property type="entry name" value="neural-cadherin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000035">
        <property type="entry name" value="Protocadherin Fat 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000215">
        <property type="entry name" value="Si:dkey-22o22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000196">
        <property type="entry name" value="Si:dkey-22o22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000224">
        <property type="entry name" value="Si:dkey-22o22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000234">
        <property type="entry name" value="Si:dkey-22o22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056370">
        <property type="entry name" value="Shg-like_Ig-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF432">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24811">
        <property type="entry name" value="Ig_Shg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 13 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 15" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019532702" description="Neural-cadherin-like" evidence="18">
        <location>
            <begin position="29"/>
            <end position="2307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="2111"/>
            <end position="2131"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="26"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="106"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="224"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="322"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="422"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="534"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="649"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="751"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="861"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="972"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1081"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1185"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="1549"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="19">
        <location>
            <begin position="1589"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="1793"/>
            <end position="1832"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="19">
        <location>
            <begin position="1835"/>
            <end position="2012"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="2057"/>
            <end position="2094"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="1578"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="1822"/>
            <end position="1831"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="2084"/>
            <end position="2093"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2307" mass="255946" checksum="1A2839013A9B3CF7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQL7</accession>
    <name>A0A452HQL7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FERM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQL7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017336"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019780.1">
        <property type="protein sequence ID" value="ENSGAGP00000017336.1"/>
        <property type="gene ID" value="ENSGAGG00000012907.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13186">
        <property type="entry name" value="FERM_C_NBL4_NBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000003">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF18">
        <property type="entry name" value="BAND 4.1-LIKE PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="4">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="389" mass="45429" checksum="4BFFD2B37109AB04" modified="2019-05-08" version="1">MDASQVTHWLDHSKPIKKQIKIGPPYTLHFRIKYYSSEPNNLHEEFTRYLFVLQLRQDILSGKLKCPYDTAVELAALCLQAELGECEHPEHTPELVSEFRFTPNQTEAMEFDIFQKWKECRGKTPAQAELCYLNKAKWLEMYGVDMHVVKGRDGCDYALGLTPTGVLIFEGANKIGLFFWPKITKMDFKKSKLTLVVVEDDQQGREQEHTFVFRLDNAKTCKHLWKCAVEHHAFFRLRIPANSKSSRSDFIRLGSRFRFSGRTEYQATHGSRLRRTSTFERKPSKRYPSRRHSTFKANNPVKAAQLCAKNNPEVHNYQSQYHPNIHPGPPHWHPHTPVNIRPPYHDDRPYWKPTTSGDDRNLHYIQDQNQKNLGGIQSMVYQDKLMTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HS67</accession>
    <name>A0A452HS67_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM half pint family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HS67"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017915"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020436.1">
        <property type="protein sequence ID" value="ENSGAGP00000017915.1"/>
        <property type="gene ID" value="ENSGAGG00000013289.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12370">
        <property type="entry name" value="RRM1_PUF60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12371">
        <property type="entry name" value="RRM2_PUF60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12648">
        <property type="entry name" value="RRM3_UHM_PUF60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000133">
        <property type="entry name" value="poly(U)-binding-splicing factor PUF60 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000136">
        <property type="entry name" value="poly(U)-binding-splicing factor PUF60 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000152">
        <property type="entry name" value="poly(U)-binding-splicing factor PUF60 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006532">
        <property type="entry name" value="PUF60-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051974">
        <property type="entry name" value="PUF60_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034209">
        <property type="entry name" value="PUF60_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034211">
        <property type="entry name" value="PUF60_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034212">
        <property type="entry name" value="PUF60_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01645">
        <property type="entry name" value="half-pint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47330:SF1">
        <property type="entry name" value="POLY(U)-BINDING-SPLICING FACTOR PUF60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47330">
        <property type="entry name" value="POLY(U)-BINDING-SPLICING FACTOR PUF60-B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="138"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="235"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="471"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="568" mass="61185" checksum="065190A25C06CDDE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSA6</accession>
    <name>A0A452HSA6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HSA6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017969"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020495.1">
        <property type="protein sequence ID" value="ENSGAGP00000017969.1"/>
        <property type="gene ID" value="ENSGAGG00000013321.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15227">
        <property type="entry name" value="7tmA_OR14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000037">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="321" mass="36366" checksum="B57BA4DA052CA048" modified="2019-05-08" version="1">MTNQTSVTEFRLLGFSDIRELQILHFLVFLFAYVAAFMGNFLIIVLVTLDHHLHSPMYFFLKNMSFLDLCYISATVPKSMVNAIMQCHSISFHGCFLQLFFFITFGAAELALLTVMAYDRYLAICSPLRYRTVMNSRACVQMAAGSCLSGALYSVLHVGNSFSLPFCGPNFVHQFFCDIPPLLQLSCSDTQPNKVHNIVLALCYGLICFTFIFVSYIHIFNTVVRIPSAEGRYKAFSTCLPHLIVVSLFIGTGFFMYMKPNPRVPSTQDMLAPVLYAVVPPVLNPIIYSLRNKEIKGALRKLLFNLFKVFIWNIPSKPFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSW5</accession>
    <name>A0A452HSW5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HSW5"/>
    <dbReference type="SMR" id="A0A452HSW5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018157"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020695.1">
        <property type="protein sequence ID" value="ENSGAGP00000018157.1"/>
        <property type="gene ID" value="ENSGAGG00000013450.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16127">
        <property type="entry name" value="Ubl_ATG8_GABARAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000037">
        <property type="entry name" value="Gamma-aminobutyric acid receptor-associated protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="10">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="117" mass="14044" checksum="7F56E345255F564B" modified="2019-05-08" version="1">MKFQYKEDHPFEYRKKEGEKIRKKYPDRVPVIVEKAPKARVPDLDKRKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNTIPPTSATMGQLYEDNHEEDYFLYVAYSDESVYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HT12</accession>
    <name>A0A452HT12_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Band 4.1-like protein 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Erythrocyte membrane protein band 4.1-like 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in the formation and organization of tight junctions during the establishment of polarity in epithelial cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of a complex composed of PALS1, CRB1 and EPB41L5. Within the complex, interacts (via FERM domain) with PALS1 (via HOOK domain) and with CRB1 (via intracellular domain). Interacts with CRB2 (via intracellular domain). Interacts with CRB3 (via intracellular domain).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Photoreceptor inner segment</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HT12"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018224"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020771.1">
        <property type="protein sequence ID" value="ENSGAGP00000018224.1"/>
        <property type="gene ID" value="ENSGAGG00000013498.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000020773.1">
        <property type="protein sequence ID" value="ENSGAGP00000018226.1"/>
        <property type="gene ID" value="ENSGAGG00000013498.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000020780.1">
        <property type="protein sequence ID" value="ENSGAGP00000018233.1"/>
        <property type="gene ID" value="ENSGAGG00000013498.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13186">
        <property type="entry name" value="FERM_C_NBL4_NBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17205">
        <property type="entry name" value="FERM_F1_EPB41L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000024">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1-like 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000003">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF15">
        <property type="entry name" value="BAND 4.1-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="14">
        <location>
            <begin position="43"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="353"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="766" mass="85165" checksum="7002176976B7364E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HW29</accession>
    <name>A0A452HW29_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HW29"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019342"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022028.1">
        <property type="protein sequence ID" value="ENSGAGP00000019342.1"/>
        <property type="gene ID" value="ENSGAGG00000014273.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00229">
        <property type="entry name" value="SGNH_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051532">
        <property type="entry name" value="Ester_Hydrolysis_Enzymes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30383:SF5">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30383">
        <property type="entry name" value="THIOESTERASE 1/PROTEASE 1/LYSOPHOSPHOLIPASE L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="33888" checksum="FD4A71BB79B2B88D" modified="2019-05-08" version="1">MMEHRYEEAEGISSDLQMEAGPKNSEERLLGEESGRWKHVTKRTRQRKRRASEGEIELRNRFAELENEEGAQQVVAEGVRARKKRRAASPTGRGEESMETTPNMSPRRIREGLRRTARVNRNREDLQPVGAGDRPENHTVTRKRQVYVIGDSLLRRIDRLVTRADPENRRVCCLPGAKIWDVHLRLKRILTGVGKNPLIVLHVGTNDTARFSLECIKGDYTRLWKTLKEIEAQVIFSGILPVPREGQQRCDRIMGFNRWLRQWCYKEGFRMYGHWEAFMDRRLFSGMDFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I258</accession>
    <name>A0A452I258_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I258"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021590"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024590.1">
        <property type="protein sequence ID" value="ENSGAGP00000021590.1"/>
        <property type="gene ID" value="ENSGAGG00000015843.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000165">
        <property type="entry name" value="TBC1 domain family member 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17006" checksum="27B0783161EB7F4D" modified="2019-05-08" version="1">MEPLSFKVKKLQAPTDISVDFSLQLVGSLPVHSLTTMPVLPWIVAEIRRLSMQSSIEEPSTSQIRLYVSPAGLRCEPDTGKSQQWDPLICSSLFEYKPQHVHKLIHNSHDPSYFACLIKDGAVNQHSICYVFRAADPTKVSKKAILRACFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I261</accession>
    <name>A0A452I261_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000021574"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024568.1">
        <property type="protein sequence ID" value="ENSGAGP00000021574.1"/>
        <property type="gene ID" value="ENSGAGG00000015819.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008024">
        <property type="term" value="C:cyclin/CDK positive transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07864">
        <property type="entry name" value="STKc_CDK12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000102">
        <property type="entry name" value="cyclin-dependent kinase 12 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000074">
        <property type="entry name" value="cyclin-dependent kinase 12 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF459">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="676"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="87"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="620"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="996"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1111"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1185"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1357"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1464"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="46"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="302"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="344"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="406"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="459"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="483"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="631"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1014"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1027"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1123"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1187"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="1233"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="705"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1490" mass="163126" checksum="F2BA421718C92985" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2B2</accession>
    <name>A0A452I2B2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HEXIM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I2B2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021629"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024636.1">
        <property type="protein sequence ID" value="ENSGAGP00000021629.1"/>
        <property type="gene ID" value="ENSGAGG00000015869.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097322">
        <property type="term" value="F:7SK snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024872">
        <property type="entry name" value="HEXIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13469">
        <property type="entry name" value="HEXAMETHYLENE BISACETAMIDE INDUCIBLE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13469:SF8">
        <property type="entry name" value="HEXIM P-TEFB COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15313">
        <property type="entry name" value="HEXIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02094">
        <property type="entry name" value="HEXIMFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="94"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="164"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="241" mass="28268" checksum="4429A864BC00C527" modified="2019-05-08" version="1">ISKSFMEPPNSPCRAPGGGEEEWRQRQLGKKKHRRRPSKKKRHWKPYYKLTWEEKKLFDERQSQRASRLRAEMFAKGQPVAPYNTTQFLMEDHDQEEPDLKTGLYPKKAAAKSDDTSEEDFLEEAADEDGGSDGMGGDGSEFLQRDFSETYERYHVESLQNMSKQELIKEYLELEKCLSRMEDENNRLRLESKKYGGGSAEDARVRELELELDRLRAENLKLLKENELHRQQEKSLSKFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2J5</accession>
    <name>A0A452I2J5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with PPP1CA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I2J5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021722"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024745.1">
        <property type="protein sequence ID" value="ENSGAGP00000021722.1"/>
        <property type="gene ID" value="ENSGAGG00000015943.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045184">
        <property type="term" value="P:establishment of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099560">
        <property type="term" value="P:synaptic membrane adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000047">
        <property type="entry name" value="protein phosphatase 1 regulatory subunit 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055106">
        <property type="entry name" value="ELFN_Fn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF204">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22986">
        <property type="entry name" value="Fn3_ELFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019153091" description="LRRCT domain-containing protein" evidence="14">
        <location>
            <begin position="23"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="15">
        <location>
            <begin position="185"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="584"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="801" mass="89390" checksum="13A6CF4B5579FD8E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5W2</accession>
    <name>A0A452I5W2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I5W2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022987"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026194.1">
        <property type="protein sequence ID" value="ENSGAGP00000022987.1"/>
        <property type="gene ID" value="ENSGAGG00000016861.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000109">
        <property type="entry name" value="WD repeat domain 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000139">
        <property type="entry name" value="WD repeat domain 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059157">
        <property type="entry name" value="WDR36-Utp21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007319">
        <property type="entry name" value="WDR36/Utp21_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840:SF12">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25171">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25168">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04192">
        <property type="entry name" value="Utp21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR36/Utp21 N-terminal" evidence="5">
        <location>
            <begin position="51"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="282"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="490"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="573"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="WDR36/Utp21 C-terminal" evidence="4">
        <location>
            <begin position="699"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="905" mass="99583" checksum="BB7041856A222C34" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5W9</accession>
    <name>A0A452I5W9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I5W9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022971"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026177.1">
        <property type="protein sequence ID" value="ENSGAGP00000022971.1"/>
        <property type="gene ID" value="ENSGAGG00000016819.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000279">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="36"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="274"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="377" mass="41610" checksum="073E08CFB70F633F" modified="2019-05-08" version="1">MLKVTMPSSSSSSAAAGRAPDYWIDGSNKDTLAHYFELESELGRGATSIVYRCRQKGTQKPYAVKMLKKTVDKKIVRTEIGVLLRLSHPNIIKLKEIFETPTEISLVLELVTGGELFDRIVEKGYYSERDAADAVKQILEAVAYLHANGIVHRDLKPENLLYATPAPDAPLKIADFGLSKIVEDQVTMKTVCGTPGYCAPEILRGCAYGPEVKKLIVFDAKKRLTTFQALQHPWVTGKAANFAHMDNAQKKLQEFNARRKLKAAVKAVVASTRLGSASGHSSHDSNKPSHNPSPVQDSEPEEKPTQEGEVICHFMHQLSCHSARKICKPGLSGRSALECKMHQLVVLTSMHVTAYENSKLSYQACHGYSVIFCNTNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6L8</accession>
    <name>A0A452I6L8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I6L8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023257"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026496.1">
        <property type="protein sequence ID" value="ENSGAGP00000023257.1"/>
        <property type="gene ID" value="ENSGAGG00000017056.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052220">
        <property type="entry name" value="METTL25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12496">
        <property type="entry name" value="CGI-41 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12496:SF9">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13679">
        <property type="entry name" value="Methyltransf_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="154"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="595" mass="66955" checksum="D46062777430F04A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6S9</accession>
    <name>A0A452I6S9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Butyrophilin subfamily 1 member A1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. BTN/MOG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I6S9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023342"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026589.1">
        <property type="protein sequence ID" value="ENSGAGP00000023342.1"/>
        <property type="gene ID" value="ENSGAGG00000017112.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000088">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000208">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053896">
        <property type="entry name" value="BTN3A2-like_Ig-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF149">
        <property type="entry name" value="BG-LIKE ANTIGEN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22705">
        <property type="entry name" value="C2-set_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="9"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="129"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="259"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="55616" checksum="0AC160C7A3B4F169" modified="2019-05-08" version="1">VLVSLPLSPVSFQVLVPAGPLSAPLGGTVLLSCHLSPALSAQAMQVKWSRPQLGQDVHVYLPDGTEVQGERYRGRTELLRDRIQSGSLALRIWNLTLRDEGRYLCDFQSDTYFSDATLELHLTSSGSDPLLDVSLYKDQGVSVTCLSAGWYPEPNVLWRNSHQKILTPESEQKLQSDDGIFNVSSTVVITESSDPALTCTLSPGSPGPEKVSAIVISGELLMKQRITELEPDLQWWRMAFIDSENFMTGTIPYCVSVQCPVQQALISITVCLCSARPDVTLDPRTAHPSLELSAERKSLRNTGPRQSLPDGLGRFDVYSCVLGSDGYESGTHYWDVEVASTGGWALGVTRESASRKGWFNFSPRQGTWAIQLSRGQYREVTAEWSLLDQSQSPGKIRVYLDYEGGVVCFYDADTMALLHTFTAFFTGKIYPFFWVWSIAAHANLAPPVLGSLCFPPPEGSACLPGAESPGIGAEAWPVLASCGGTVWGLGSAHPGTWVLQEPAQGRPWPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7I7</accession>
    <name>A0A452I7I7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I7I7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023590"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026878.1">
        <property type="protein sequence ID" value="ENSGAGP00000023590.1"/>
        <property type="gene ID" value="ENSGAGG00000017290.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001230">
        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="19"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="129" mass="13934" checksum="6C0FD77D3FB88B11" modified="2019-05-08" version="1">MELSVLYSALVNSFGQKVGQIVMTQTPESLAVSPGDRVTINCKASSSTSNYVAWYQQKSGQAPKLLIYAATSRYTGVPARFSGSGSGSGYTDYTLTISSVEVEDAGDYYCQYSYSSPLTKSGTKCEQWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I819</accession>
    <name>A0A452I819_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coiled-coil domain containing 107</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I819"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023782"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027088.1">
        <property type="protein sequence ID" value="ENSGAGP00000023782.1"/>
        <property type="gene ID" value="ENSGAGG00000017406.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038779">
        <property type="entry name" value="CCDC107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37345">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37345:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="20103" checksum="C1B98BD86064B654" modified="2019-05-08" version="1">MSNTLSSLLEHQLLELEQHLAQTEKILNSLLTQLDPLSNCVNALACEQKDEIMTQLQSIRQLMKESGLDKSEVKPKDVNHTYNEKLEDLIQSFGKHTQEKADDSEDRSIEDLLEDTAGCSRKEEHELCDCEFTLNQLIEPKVIEVQEIINREILDPEETELRRRNKYELNMHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IA79</accession>
    <name>A0A452IA79_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Paraneoplastic antigen Ma-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IA79"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024599"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027996.1">
        <property type="protein sequence ID" value="ENSGAGP00000024599.1"/>
        <property type="gene ID" value="ENSGAGG00000017963.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026523">
        <property type="entry name" value="PNMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048271">
        <property type="entry name" value="PNMA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095:SF18">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20846">
        <property type="entry name" value="PNMA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Paraneoplastic antigen Ma-like N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="77" mass="8533" checksum="6421E4B1DBECB6B2" modified="2019-05-08" version="1">MVCIMALHLLEDWCKGMNIDPRNCLLIMGVLEAVDEGSIEPILRSSIEYLCKCKMRGSIFVREEGAFAGLCELPSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAC1</accession>
    <name>A0A452IAC1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TBC1 domain family member 20</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IAC1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024613"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028011.1">
        <property type="protein sequence ID" value="ENSGAGP00000024613.1"/>
        <property type="gene ID" value="ENSGAGG00000017867.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000024">
        <property type="entry name" value="TBC1 domain family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1310:FF:000001">
        <property type="entry name" value="TBC1 domain family, member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045913">
        <property type="entry name" value="TBC20/Gyp8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913:SF11">
        <property type="entry name" value="RAB-GAP TBC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 20/GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="7">
        <location>
            <begin position="58"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="403" mass="45457" checksum="F3324962907E9A8E" modified="2019-05-08" version="1">SALGGQQFHHPVRWSRGSGGALLGSKRKQKLLLIHQALTSDPVAVETLRGAAVSEGGLLTDEIRRKVWPKLLSVNVYNLPPKPGRTVRQNHKDYTQVQLDVNRSTHRFPQGMRAEQRRVLQEQLIDLILHILRAHPELHYYQGYHDIAVTLLLVAGERMGSALLEKLSTHHLRDFMDPTMDSTKHILNYLMPILQQESLRLHDFMLRYQSRSWVGTIFALSWLITWYGHVLTSFPHILRLYDFFLASHPLMPVYFAAVIVLHREAEVLACDCDMPSVHQLLSQIPQNLPYERLISWALQLFQRHPHAELAKQAALQGSQTAAQAGSFSAFQLATTQQRPDAVLRRQRQQDSAHAETAALLPTAGHSPLVKAAVWGISATLGAVALAVTQTALEWAPEFLLQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB89</accession>
    <name>A0A452IB89_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tissue factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Coagulation factor III</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tissue factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IB89"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024957"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028410.1">
        <property type="protein sequence ID" value="ENSGAGP00000024957.1"/>
        <property type="gene ID" value="ENSGAGG00000018250.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000899">
        <property type="entry name" value="Tissue factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015373">
        <property type="entry name" value="Interferon/interleukin_rcp_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001187">
        <property type="entry name" value="Tissue_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050650">
        <property type="entry name" value="Type-II_Cytokine-TF_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859">
        <property type="entry name" value="INTERFERON/INTERLEUKIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859:SF22">
        <property type="entry name" value="TISSUE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09294">
        <property type="entry name" value="Interfer-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01108">
        <property type="entry name" value="Tissue_fac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00346">
        <property type="entry name" value="TISSUEFACTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0564">Palmitate</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019280092" description="Tissue factor" evidence="18">
        <location>
            <begin position="31"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="16"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Interferon/interleukin receptor" evidence="20">
        <location>
            <begin position="130"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF09294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="291" mass="32901" checksum="A6AE2FC177AA6C94" modified="2019-05-08" version="1">MASALDALAAAWRRALLLAALLSRLDPCSGNSELSTAVNITWSSINFKTILQWQPVPTNYVYTVKISGINSNWEKACVHTKETECDVTDKLEKVKDTYTAHIVSEMLSGTEKFEEPPYAISPKFTPYNQTTIGKPGIQTYELTDSKLKVLVEDPLTPYRFPNKTFKSIREIFKNDLEYTLYYWKDLSTGKKEATTKSNQFEISIDKGENYCFYVQATILSRRENRKSQESNMNCTRHWEDGPAAYATEVFIIIAAAAIVILITIIGLSVALYKCKKTKAAKEKETMPLNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICI1</accession>
    <name>A0A452ICI1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ICI1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025212"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028703.1">
        <property type="protein sequence ID" value="ENSGAGP00000025212.1"/>
        <property type="gene ID" value="ENSGAGG00000018444.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000681">
        <property type="entry name" value="zinc finger protein 205 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000269">
        <property type="entry name" value="Zinc finger protein 287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF377">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="54"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="410" mass="46471" checksum="8FB4C5E31CF6B0A7" modified="2019-05-08" version="1">MILWSDPVWPFLCFYVSIPLSEVPFLPGTGNDSCLDSLFCPAGDGMMSKIKEENLEREGPEEVEPHGTVLGRSKGNVSQRPKHGEACGSRFKSERQKGNQPRKRWTESTHPGEGLEDPKESMIQPKIYTKKKSYNCPECGKSFTQTSNLIIHERIHTGQRPYKCLECGKSFRQRSDLSKHQRIHTGETPYKCPDCGKSFKQRFSLIVHHRSHTGETPYKCNDCGKGFTQSTNLVSHQRIHTGETPYKCTDCGKSFKQRFSLIVHQRNHTGERAYKCADCGKSFKQSSELISHQRIHVGERPFKCTECGKNFSQSSGLIRHRRTHTGEKPYKCIDCGKGFSQSSGLIRHRRTHTGQKPYDCLECGKSFTQSSALIVHQRVHTGEAPYRCSVCGKSYKVKDSLISHQKLHTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ID97</accession>
    <name>A0A452ID97_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">F-actin-capping protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions. Forms, with CAPZB, the barbed end of the fast growing ends of actin filaments in the dynactin complex and stabilizes dynactin structure. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">Z line</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the F-actin-capping protein alpha subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ID97"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025680"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029215.1">
        <property type="protein sequence ID" value="ENSGAGP00000025680.1"/>
        <property type="gene ID" value="ENSGAGG00000018748.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030863">
        <property type="term" value="C:cortical cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008290">
        <property type="term" value="C:F-actin capping protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.60:FF:000001">
        <property type="entry name" value="F-actin-capping protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.210:FF:000002">
        <property type="entry name" value="F-actin-capping protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.60">
        <property type="entry name" value="F-actin capping protein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.210">
        <property type="entry name" value="F-actin capping protein, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002189">
        <property type="entry name" value="CapZ_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037282">
        <property type="entry name" value="CapZ_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042276">
        <property type="entry name" value="CapZ_alpha/beta_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042489">
        <property type="entry name" value="CapZ_alpha_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017865">
        <property type="entry name" value="F-actin_cap_asu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653:SF5">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01267">
        <property type="entry name" value="F-actin_cap_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00191">
        <property type="entry name" value="FACTINCAPA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90096">
        <property type="entry name" value="Subunits of heterodimeric actin filament capping protein Capz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00748">
        <property type="entry name" value="F_ACTIN_CAPPING_A_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00749">
        <property type="entry name" value="F_ACTIN_CAPPING_A_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0117">Actin capping</keyword>
    <keyword evidence="6 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="286" mass="33011" checksum="564B6D4D3DC3EB90" modified="2019-05-08" version="1">MADFEDRVSDEEKVRIAAKFITHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNMDQFTPVKIEGYDDQVLITEHGDLGNGRFLDPRNKLSFKFDHLRKEASDPHHEDTEAALKPWRDACDSALRAYVKDHYPNGFCTVYGKTIDGQQTIIACIESHQFQPKNFWNGRWRSEWKFTITPPAAQVVAVLKIQVHYYEDGNVQLVSHKDIQDSLTVSNDVQTAKEFIKIIEHAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IH50</accession>
    <name>A0A452IH50_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PEHE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IH50"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026794"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030456.1">
        <property type="protein sequence ID" value="ENSGAGP00000026794.1"/>
        <property type="gene ID" value="ENSGAGG00000019517.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044545">
        <property type="term" value="C:NSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035035">
        <property type="term" value="F:histone acetyltransferase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026180">
        <property type="entry name" value="NSL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029332">
        <property type="entry name" value="PEHE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22443:SF16">
        <property type="entry name" value="KAT8 REGULATORY NSL COMPLEX SUBUNIT 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22443">
        <property type="entry name" value="NON-SPECIFIC LETHAL 1, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15275">
        <property type="entry name" value="PEHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01300">
        <property type="entry name" value="PEHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52052">
        <property type="entry name" value="PEHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PEHE" evidence="2">
        <location>
            <begin position="807"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="524"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="705"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="542"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1010" mass="114588" checksum="A8BFF5EBA620D368" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IH96</accession>
    <name>A0A452IH96_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endothelin receptor type B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Endothelin receptor non-selective type</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IH96"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027276"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030984.1">
        <property type="protein sequence ID" value="ENSGAGP00000027276.1"/>
        <property type="gene ID" value="ENSGAGG00000019835.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004962">
        <property type="term" value="F:endothelin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048066">
        <property type="term" value="P:developmental pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048484">
        <property type="term" value="P:enteric nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008217">
        <property type="term" value="P:regulation of blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042310">
        <property type="term" value="P:vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15976">
        <property type="entry name" value="7tmA_ET-BR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000076">
        <property type="entry name" value="Endothelin receptor type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000499">
        <property type="entry name" value="Endthln_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001112">
        <property type="entry name" value="ETB_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051193">
        <property type="entry name" value="GPCR_endothelin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46099:SF3">
        <property type="entry name" value="ENDOTHELIN RECEPTOR TYPE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46099">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00571">
        <property type="entry name" value="ENDOTHELINBR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00366">
        <property type="entry name" value="ENDOTHELINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="11 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019249779" description="Endothelin receptor type B" evidence="17">
        <location>
            <begin position="22"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="355"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="112"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="435" mass="48860" checksum="94F021BBF138FC32" modified="2019-05-08" version="1">MHRPSLWSLLAPALLLSCCAAWEEKGSLRPRGVSQRSPLSPGAAVTHTARTPWLRGSASASNVSSSAVPGNDPGRARSLSPPMCTGQTEVKETFKYINTLVSCLVFVLGIIGNSTLLRIIYKNKCMRNGPNILIASLALGDLLHITIAIPVNVYKLLVMDWPFGVEMCKLVPFIQKASVGITVLSLCALSIDRYRAVASWSRIKGIGVPKWTAIEIVLIWVMSVVLAVPEAIGFDMIVMEYKGKHLRICMLHPTQKTSFMQFYKTAKDWWLFSFYFCLPLAITALFYTLMTCKMLRKKSGMQIALNDHLKQRREVAKTVFCLVLVFALCWLPLHLSRILKLTVYDQRDPNRCELLSFFLVLDYIGINMASLNSCINPIALYLVSKRFKNCFKSCLCCCCQSKDMLSLEEKQSCLKFKAEDHGYDNFRSSNKYSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHM2</accession>
    <name>A0A452IHM2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IHM2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026979"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030661.1">
        <property type="protein sequence ID" value="ENSGAGP00000026979.1"/>
        <property type="gene ID" value="ENSGAGG00000019636.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19839">
        <property type="entry name" value="Bbox1_TRIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12890">
        <property type="entry name" value="SPRY_PRY_TRIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF10">
        <property type="entry name" value="TRIPARTITE MOTIF-CONTAINING PROTEIN 16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="5">
        <location>
            <begin position="348"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="559" mass="63515" checksum="47EF83B5BDB2F2CE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHU6</accession>
    <name>A0A452IHU6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cohesin subunit SA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">SCC3 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Stromal antigen</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the cohesin complex which is composed of a heterodimer between a SMC1 protein (SMC1A or SMC1B) and SMC3, which are attached via their hinge domain, and RAD21 which link them at their heads, and one STAG protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the SCC3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IHU6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027252"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030957.1">
        <property type="protein sequence ID" value="ENSGAGP00000027252.1"/>
        <property type="gene ID" value="ENSGAGG00000019816.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000030961.1">
        <property type="protein sequence ID" value="ENSGAGP00000027256.1"/>
        <property type="gene ID" value="ENSGAGG00000019816.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056396">
        <property type="entry name" value="HEAT_SCC3-SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013721">
        <property type="entry name" value="STAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF6">
        <property type="entry name" value="COHESIN SUBUNIT SA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24571">
        <property type="entry name" value="HEAT_SCC3-SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21581">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08514">
        <property type="entry name" value="STAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51425">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCD" evidence="5">
        <location>
            <begin position="297"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1057"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="261"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1063"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1077"/>
            <end position="1088"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1258" mass="144342" checksum="53A8F55F587DFC84" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIF1</accession>
    <name>A0A452IIF1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MICOS complex subunit MIC60</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mitofilin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the MICOS complex subunit Mic60 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IIF1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027647"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031409.1">
        <property type="protein sequence ID" value="ENSGAGP00000027647.1"/>
        <property type="gene ID" value="ENSGAGG00000020093.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019133">
        <property type="entry name" value="MIC60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415:SF7">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415">
        <property type="entry name" value="MITOFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09731">
        <property type="entry name" value="Mitofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="118"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="285"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="508"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="764" mass="84583" checksum="BA65E10520A48CD5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452III2</accession>
    <name>A0A452III2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SOCS box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452III2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027665"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031431.1">
        <property type="protein sequence ID" value="ENSGAGP00000027665.1"/>
        <property type="gene ID" value="ENSGAGG00000020107.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03722">
        <property type="entry name" value="SOCS_ASB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050776">
        <property type="entry name" value="Ank_Repeat/CDKN_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037329">
        <property type="entry name" value="ASB3_SOCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201:SF2">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="78"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="111"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="145"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="178"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="211"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="5">
        <location>
            <begin position="458"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="540" mass="60517" checksum="118792600FC1B64C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJT5</accession>
    <name>A0A452IJT5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Nuclear transport factor 2</fullName>
            <shortName evidence="5">NTF-2</shortName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Has a role in nuclear-cytoplasmic transport of proteins and mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJT5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028196"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032042.1">
        <property type="protein sequence ID" value="ENSGAGP00000028196.1"/>
        <property type="gene ID" value="ENSGAGG00000020494.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000007">
        <property type="entry name" value="Nuclear transport factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="6">
        <location>
            <begin position="10"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="127" mass="14639" checksum="03368B12F236B4E8" modified="2019-05-08" version="1">MAERPMWEQVGTSFVHLYYQRFDSSRVELSALYTDASCLSWEGQQFQGKTAIMEKLMSLPFQKIQHSITSQDHQPAPDNCILSMVVGQLKVDNDPVMGFHQLFVLKNISDNWVCTNDIFRLALYNFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKX1</accession>
    <name>A0A452IKX1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAS-associated death domain protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">FAS-associating death domain-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Fas-associated death domain protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Apoptotic adapter molecule that recruits caspases CASP8 or CASP10 to the activated FAS/CD95 or TNFRSF1A/TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. Active CASP8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Can self-associate. Component of the AIM2 PANoptosome complex, a multiprotein complex that drives inflammatory cell death (PANoptosis). Component of the death-induced signaling complex (DISC) composed of cell surface receptor FAS/CD95 or TNFRSF1A, adapter protein FADD and the CASP8 protease; recruitment of CASP8 to the complex is required for processing of CASP8 into the p18 and p10 subunits. Interacts (via death domain) with FAS (via death domain). Interacts directly (via DED domain) with NOL3 (via CARD domain); inhibits death-inducing signaling complex (DISC) assembly by inhibiting the increase in FAS-FADD binding induced by FAS activation. Interacts with CFLAR, PEA15 and MBD4. When phosphorylated, part of a complex containing HIPK3 and FAS. May interact with MAVS/IPS1. Interacts with MOCV v-CFLAR protein and PIDD1. Interacts with RIPK1 and TRADD. Interacts with stimulated TNFRSF10B. Interacts with DDX24.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IKX1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028535"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032406.1">
        <property type="protein sequence ID" value="ENSGAGP00000028535.1"/>
        <property type="gene ID" value="ENSGAGG00000020668.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031265">
        <property type="term" value="C:CD95 death-inducing signaling complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089720">
        <property type="term" value="F:caspase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005123">
        <property type="term" value="F:death receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097191">
        <property type="term" value="P:extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001819">
        <property type="term" value="P:positive regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001238">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045089">
        <property type="term" value="P:positive regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08306">
        <property type="entry name" value="Death_FADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08336">
        <property type="entry name" value="DED_FADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000059">
        <property type="entry name" value="Fas-associated via death domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000062">
        <property type="entry name" value="Fas-associated via death domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001875">
        <property type="entry name" value="DED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016729">
        <property type="entry name" value="FADD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15077:SF10">
        <property type="entry name" value="FAS-ASSOCIATED DEATH DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15077">
        <property type="entry name" value="FAS-ASSOCIATING DEATH DOMAIN-CONTAINING PROTEIN FADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01335">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00031">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50168">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DED" evidence="13">
        <location>
            <begin position="3"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="12">
        <location>
            <begin position="102"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="191" mass="22181" checksum="578E64AC4C6AD7C9" modified="2019-05-08" version="1">MDPFLSLLHSLSVSLSDNELSALKFLCLSKIGKRKLESVKTGSDLFTILLEQREIEREKVDFLQLMIKTIKREDLIMQLQEFIEGGQGDVVNHLDEKEKRQQNVAFEVICDNVGKNWKMLVRRLGISDAKIDRILTANPYNLQEQLMQSLREWQKWKGKEAKAADLIKALRDCKMNLVADKVEHELLQRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IL03</accession>
    <name>A0A452IL03_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Extended synaptotagmin-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. Helps tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane. Acts as an inhibitor of ADGRD1 G-protein-coupled receptor activity in absence of cytosolic calcium. Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with ESYT2 and ESYT3. Interacts with ADGRD1; inhibiting the G-protein-coupled receptor activity of ADGRD1. Interaction with ADGRD1 is abolished when cytosolic calcium increases, relieving ADGRD1 G-protein-coupled receptor activity. Interacts (phosphorylated form) with SLC2A4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the extended synaptotagmin family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000028501"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032371.1">
        <property type="protein sequence ID" value="ENSGAGP00000028501.1"/>
        <property type="gene ID" value="ENSGAGG00000020597.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008429">
        <property type="term" value="F:phosphatidylethanolamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08391">
        <property type="entry name" value="C2A_C2C_Synaptotagmin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd04050">
        <property type="entry name" value="C2B_Synaptotagmin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd04030">
        <property type="entry name" value="C2C_KIAA1228"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000025">
        <property type="entry name" value="Extended synaptotagmin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000106">
        <property type="entry name" value="extended synaptotagmin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000120">
        <property type="entry name" value="extended synaptotagmin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000139">
        <property type="entry name" value="extended synaptotagmin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037752">
        <property type="entry name" value="C2C_KIAA1228"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037733">
        <property type="entry name" value="Ext_Synaptotagmin_C2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037749">
        <property type="entry name" value="Ext_Synaptotagmin_C2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051634">
        <property type="entry name" value="Extended_Synaptotagmin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039010">
        <property type="entry name" value="Synaptotagmin_SMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45761:SF3">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45761">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-LIKE PROTEIN 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17047">
        <property type="entry name" value="SMP_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="15" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="22">
        <location>
            <begin position="58"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="21">
        <location>
            <begin position="269"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="21">
        <location>
            <begin position="413"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="21">
        <location>
            <begin position="576"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="21">
        <location>
            <begin position="731"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="21">
        <location>
            <begin position="925"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="867"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="872"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00093357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00093580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1047" mass="116098" checksum="D1E9DF5B4A2B8FA5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILQ3</accession>
    <name>A0A452ILQ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILQ3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028767"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032682.1">
        <property type="protein sequence ID" value="ENSGAGP00000028767.1"/>
        <property type="gene ID" value="ENSGAGG00000020848.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000122">
        <property type="entry name" value="Chymotrypsin-like elastase family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050850">
        <property type="entry name" value="Peptidase_S1_Elastase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24257">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE ELASTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24257:SF0">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE ELASTASE FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="6"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="81" mass="9192" checksum="4816FA8F9FBC2C45" modified="2019-05-08" version="1">MRRVFISLQYYSGGQWYHTCGGTLVRQNWVMTAAHCVDRSLTFRVVVGEYNLNQNDGTEQYLSVASKFIHSSWNSNNVAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IM59</accession>
    <name>A0A452IM59_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SPOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IM59"/>
    <dbReference type="SMR" id="A0A452IM59"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028995"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032937.1">
        <property type="protein sequence ID" value="ENSGAGP00000028995.1"/>
        <property type="gene ID" value="ENSGAGG00000020995.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21550">
        <property type="entry name" value="SPOC_RBM15B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.10:FF:000003">
        <property type="entry name" value="RNA-binding motif protein 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010912">
        <property type="entry name" value="SPOC_met"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50917">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="SPOC" evidence="5">
        <location>
            <begin position="34"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="214" mass="23605" checksum="E2EAC2F8C51E5EBA" modified="2019-05-08" version="1">MPKRRDSERNHRTGESESKTHEEPKSETKKVKNLSEFAQTLQLAWNGLLVLKNSCFPTSMHILEGDLGVINGLLKDHSSGGKLTQLKIAQRLRLDQPKLDEVTRRIKQGSPNGYAVLLATQATQGGVGAEGTFPVVEPGLQRRLLRNLVSYLKQKQAAGVISLPVGGAKCRDSTGMLYAFPPCEFSQQYLQSALRTLGKLEEEHMVIVIVKDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452INY6</accession>
    <name>A0A452INY6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Neuropeptide W</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neuropeptide B/W family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452INY6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029616"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033630.1">
        <property type="protein sequence ID" value="ENSGAGP00000029616.1"/>
        <property type="gene ID" value="ENSGAGG00000021388.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007631">
        <property type="term" value="P:feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013297">
        <property type="entry name" value="Neuropept_BW_pre"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28553">
        <property type="entry name" value="NEUROPEPTIDE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28553:SF2">
        <property type="entry name" value="NEUROPEPTIDE W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15180">
        <property type="entry name" value="NPBW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01888">
        <property type="entry name" value="NROPEPTIDEBW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019386142" description="Neuropeptide W" evidence="7">
        <location>
            <begin position="29"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="61"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="140"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="18743" checksum="EF31D7F797E97344" modified="2019-05-08" version="1">MNLRQASGGAWKTLSLLGLMLLVDPVGAWYKHVASPRYHTVGRASGLLMGIRRSPYLWRRDLGDEPGESPGSPPTSVNREPRLLHSRRQDLRAAGPRTQASGMPAPSPARGELRGREPAALTRLGLRDLIARRTIAQHQAPLQTGASLLPSAASPAGSLEPRRGPGGEEASSRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IP90</accession>
    <name>A0A452IP90_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acrosin-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acrosin-binding protein, 60 kDa form</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Proacrosin-binding protein sp32</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Acrosomal protein that maintains proacrosin (pro-ACR) as an enzymatically inactive zymogen in the acrosome. Involved also in the acrosome formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Acrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IP90"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033731.1">
        <property type="protein sequence ID" value="ENSGAGP00000029709.1"/>
        <property type="gene ID" value="ENSGAGG00000021441.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009865">
        <property type="entry name" value="Proacrosin-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21362">
        <property type="entry name" value="ACROSIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21362:SF1">
        <property type="entry name" value="ACROSIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07222">
        <property type="entry name" value="PBP_sp32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Kazal-like" evidence="10">
        <location>
            <begin position="382"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="149"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="448" mass="50430" checksum="6E752354614C1FB1" modified="2025-10-08" version="2">MPKPRLLLPMVQPVWSMSSLVPGSPLSDSEYQVFFSALMPSWKAGVACQIRRTQGCHDPKVIRLDQFENHGWIPEGPICSDLPDASWFQTFCQFAHYRCSNRKYYTKVRRGGLEQWRRWLLGGGANSCFSATPVTRRFYKELDMILGGNPHPSSPSTTVDTSEPSSTSQEEEEESESEGAEEEEDTPESLDAGSQELQCSTLCSSLKEPAGNYRSQKLTCCSFLCFLLCISLLALERDEALVILCYAMLEGICLSSAVTQAWKRMEARTLGFGDSVCDSLGRHHMELCPDCAFCSLKREQCRGVSDLRRVHCDGHSPVSNAPLCLQVGSLEAMGYYGMEVYGGLRGDYWCGRLATHGCDDPRVILWLQVEYAFFQGGDFPNKICDSDRVQHPNYCAFKSHQCLQHSLSSQKVLRYGCRRNETYQVLSEEKGEEEVLLWSQKFFSLTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPY0</accession>
    <name>A0A452IPY0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Intracellular hyaluronan-binding protein 4 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IPY0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030005"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034074.1">
        <property type="protein sequence ID" value="ENSGAGP00000030005.1"/>
        <property type="gene ID" value="ENSGAGG00000021658.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032381">
        <property type="entry name" value="IHABP4_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16174">
        <property type="entry name" value="IHABP4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Intracellular hyaluronan-binding protein 4 N-terminal" evidence="2">
        <location>
            <begin position="10"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="146" mass="15291" checksum="B5C1EA94FD4491A3" modified="2019-05-08" version="1">LPGSPVGAAMQESFGCAVANRFHQLLDDESDPFDVLREAERRQQQRRKRDEAAAAAGGRKPAPGQRPGPAAGKRESQKERKVLQPESPAAPGAPPPPPAAGGRQLNSFFPVGGRGALGAGWAVRWVPAAQGWGRPRLPPSPVLAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IU97</accession>
    <name>A0A452IU97_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IU97"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031574"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035809.1">
        <property type="protein sequence ID" value="ENSGAGP00000031574.1"/>
        <property type="gene ID" value="ENSGAGG00000022676.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040774">
        <property type="entry name" value="DUF5580"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049247">
        <property type="entry name" value="DUF5580_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049246">
        <property type="entry name" value="DUF5580_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048316">
        <property type="entry name" value="DUF5580_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34830:SF1">
        <property type="entry name" value="GENE 12695-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34830">
        <property type="entry name" value="SIMILAR TO HYPOTHETICAL PROTEIN MGC34837"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17743">
        <property type="entry name" value="DUF5580"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20743">
        <property type="entry name" value="DUF5580_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20742">
        <property type="entry name" value="DUF5580_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5580" evidence="2">
        <location>
            <begin position="171"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5580" evidence="3">
        <location>
            <begin position="292"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5580" evidence="4">
        <location>
            <begin position="396"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="566" mass="64566" checksum="DBA90C8E620AF452" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUC2</accession>
    <name>A0A452IUC2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Leucine-rich repeat-containing protein 21</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Photoreceptor-associated LRR superfamily protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Retina-specific protein PAL</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Photoreceptor synaptic protein essential for normal vision. Involved in synapse formation in cone photoreceptor cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Endomembrane system</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IUC2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031601"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035839.1">
        <property type="protein sequence ID" value="ENSGAGP00000031601.1"/>
        <property type="gene ID" value="ENSGAGG00000022695.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000058">
        <property type="entry name" value="immunoglobulin superfamily containing leucine-rich repeat protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000744">
        <property type="entry name" value="Leucine rich repeat, Ig-like and transmembrane domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000928">
        <property type="entry name" value="Leucine rich repeat, Ig-like and transmembrane domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT, IMMUNOGLOBULIN-LIKE DOMAIN AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="6 24" id="KW-0732">Signal</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0844">Vision</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019057369" description="Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 1" evidence="24">
        <location>
            <begin position="19"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="516"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="25">
        <location>
            <begin position="254"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="26">
        <location>
            <begin position="419"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="596"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00080094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="615" mass="68167" checksum="B8480D1ADA8EEFCA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUI4</accession>
    <name>A0A452IUI4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SUN domain-containing ossification factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Membrane protein CH1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">SUN-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Required for bone modeling during late embryogenesis. Regulates type I collagen synthesis in osteoblasts during their postnatal maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Rough endoplasmic reticulum membrane</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IUI4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031695"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035945.1">
        <property type="protein sequence ID" value="ENSGAGP00000031695.1"/>
        <property type="gene ID" value="ENSGAGG00000022763.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000035948.1">
        <property type="protein sequence ID" value="ENSGAGP00000031698.1"/>
        <property type="gene ID" value="ENSGAGG00000022763.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030867">
        <property type="term" value="C:rough endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046850">
        <property type="term" value="P:regulation of bone remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000024">
        <property type="entry name" value="SUN domain containing ossification factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045120">
        <property type="entry name" value="Suco/Slp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953">
        <property type="entry name" value="MEMBRANE PROTEIN CH1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953:SF0">
        <property type="entry name" value="SUN DOMAIN-CONTAINING OSSIFICATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SUN" evidence="18">
        <location>
            <begin position="182"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="29"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="551"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1059"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="841"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="559"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1069"/>
            <end position="1078"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1160" mass="129012" checksum="7A844B13A95B8FE9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IVZ4</accession>
    <name>A0A452IVZ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IVZ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032189"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036493.1">
        <property type="protein sequence ID" value="ENSGAGP00000032189.1"/>
        <property type="gene ID" value="ENSGAGG00000023041.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002360">
        <property type="entry name" value="Ras-related protein Rab-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="219" mass="24328" checksum="2D022FAD5D0A6349" modified="2019-05-08" version="1">MSYAYLFKYIIIGDTGVEFGARMINIDSKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRETFNHLTSWLEDARQHSSSNMVIMLIGNKSDLESRRDVQKAEGEAFAREHGLVFMETSAKTSTNVEEAFIDTAKEIYRKIQQGLFDINNEVSQTTPLLVPPGPHCSSAAGPCAWGVPRHCNPGCLGSHFLCQAYVAGVSAPCCYLRCLGPFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IW66</accession>
    <name>A0A452IW66_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IW66"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032268"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036583.1">
        <property type="protein sequence ID" value="ENSGAGP00000032268.1"/>
        <property type="gene ID" value="ENSGAGG00000023096.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04390">
        <property type="entry name" value="RhoGAP_ARHGAP22_24_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000015">
        <property type="entry name" value="rho GTPase-activating protein 25 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051025">
        <property type="entry name" value="RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228:SF22">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228">
        <property type="entry name" value="SPERMATHECAL PHYSIOLOGY VARIANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="7">
        <location>
            <begin position="65"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="286"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="393"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="532"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="331"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="404"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="616" mass="68101" checksum="3772F37D6C6CB4F5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IX30</accession>
    <name>A0A452IX30_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IX30"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036974.1">
        <property type="protein sequence ID" value="ENSGAGP00000032622.1"/>
        <property type="gene ID" value="ENSGAGG00000023321.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052724">
        <property type="entry name" value="GT117_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021280">
        <property type="entry name" value="TMEM260-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16214">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16214:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11028">
        <property type="entry name" value="TMEM260-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="710" mass="79304" checksum="78D702AB77767EDA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IY52</accession>
    <name>A0A452IY52_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase RNF166</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">RING finger protein 166</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">RING-type E3 ubiquitin transferase RNF166</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452IY52"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033005"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037420.1">
        <property type="protein sequence ID" value="ENSGAGP00000033005.1"/>
        <property type="gene ID" value="ENSGAGG00000023574.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16549">
        <property type="entry name" value="RING-HC_RNF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051438">
        <property type="entry name" value="RNF_E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034734">
        <property type="entry name" value="ZF_C2HC_RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016">
        <property type="entry name" value="ZINC FINGER, RING/FYVE/PHD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18574">
        <property type="entry name" value="zf_C2HC_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51803">
        <property type="entry name" value="ZF_C2HC_RNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="42"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC RNF-type" evidence="17">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="246" mass="27040" checksum="5CB368523D5AC2F0" modified="2019-05-08" version="1">MFRSLLVSAAQQRPQAPGPGGTPREQPPGGPAPDALEAQFSCPICLEVYHRAVRVAGCGHTFCGECLQPCLQVPSPLCPLCRMPFDPKKVEKASNVEKQLSSYKAPCRGCSKKVTLAKMRSHVSSCTKVQEQMANCPKFAPVVPTSQPIPSNIPNRSTFVCPYCGARNLDQQELVKHCMENHRNDPNKVVCPVCSAMPWGDPSYKSANFLQHLLHRHKFSYDTFVDYNIDEEAALQAALALSLSEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYP3</accession>
    <name>A0A452IYP3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATPase family gene 2 protein homolog B</fullName>
            <ecNumber evidence="3">3.6.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Ribosome biogenesis protein SPATA5L1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Spermatogenesis-associated protein 5-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">ATP-dependent chaperone part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability. Required for replication fork progression, sister chromatid cohesion, and chromosome stability. The ATPase activity is specifically enhanced by replication fork DNA and is coupled to cysteine protease-dependent cleavage of replisome substrates in response to replication fork damage. Uses ATPase activity to process replisome substrates in S-phase, facilitating their proteolytic turnover from chromatin to ensure DNA replication and mitotic fidelity. Plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the AAA ATPase family. AFG2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.10" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452IYP3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033210"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037634.1">
        <property type="protein sequence ID" value="ENSGAGP00000033210.1"/>
        <property type="gene ID" value="ENSGAGG00000023678.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19503">
        <property type="entry name" value="RecA-like_CDC48_NLV2_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000061">
        <property type="entry name" value="ATPase family, AAA domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000075">
        <property type="entry name" value="Spermatogenesis-associated protein 5-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001081">
        <property type="entry name" value="Spermatogenesis-associated protein 5-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF194">
        <property type="entry name" value="ATPASE FAMILY GENE 2 PROTEIN HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="227"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="493"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00084032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="715" mass="78142" checksum="7D2E096DA71A02B6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZ19</accession>
    <name>A0A452IZ19_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gonadotropin-releasing hormone receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Type II GnRH receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZ19"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033336"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037774.1">
        <property type="protein sequence ID" value="ENSGAGP00000033336.1"/>
        <property type="gene ID" value="ENSGAGG00000023764.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016500">
        <property type="term" value="F:protein-hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15383">
        <property type="entry name" value="7tmA_GnRHR_vertebrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000199">
        <property type="entry name" value="Gonadotropin-releasing hormone II receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001658">
        <property type="entry name" value="GphnRH_fam_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF22">
        <property type="entry name" value="GONADOTROPIN-RELEASING HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00529">
        <property type="entry name" value="GNADOTRPHINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="70"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="394"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="411" mass="46075" checksum="B20ADAFEED5A9B0F" modified="2019-05-08" version="1">MTHRARSGLGGGGRQGGAAMLPEGKGLKGQPPLAACWVLPSEVFLLPTFSTAAKVRVAITFVLFLSSACFNIAVLWTVTQKYRKRPHIRILIVNLAAADLLVTFVVMPLDAAWNITVQWYAGDLACRALMFLKLVAMYASAFITVVISLDRQAAILHPLSVGDAKKKNKAMLCVAWALSVLLALPQMFVFHAVSRSQPIYFIQCATVGSFQAHWQETLYNMFTFSCLFLLPLLIMVLCYSRILIEISGKMKRAFSSKEVHLRRSYNNIPRARMRTLKMSIVIVLTFILCWTPYYLLGLWYWFSPEMLSRKKVPPSLSHILFLFGLFNACLDPLIYGLFTMHFRREIRCVCCCTHHRKEQASTLIGSFRASTTATPIRRAREDQGGSGKYELEVTANSQSSALSTTPHSLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZ46</accession>
    <name>A0A452IZ46_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein mS31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">28S ribosomal protein S31, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZ46"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033353"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037793.1">
        <property type="protein sequence ID" value="ENSGAGP00000033353.1"/>
        <property type="gene ID" value="ENSGAGG00000023778.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026299">
        <property type="entry name" value="MRP-S31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13231">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13231:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15433">
        <property type="entry name" value="MRP-S31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="105"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="397" mass="44636" checksum="F787FE1D3148CF13" modified="2019-05-08" version="1">MWSRVSAVGSRRSGACRCRLLSYPWGAQDRGPGSKTQKCFSTSTGFYNKEDESATSTDSSGEKASENQEKATPGNAKKNLLNIISEMKVELSSKKTFQILKTLKTKEQTKDQPESMESASSMFQKATADSKAKRSKPLNPELVEAASAVASSLPFDRKQTVSDLLAQLRKHEEATDAQKKGETTNISNVIADMKIGKRRLTRGVSRPSSQISFDDDDGQGDMFQTGVLSEFAGSKRRGLHLGRRLNIFPVSPTVTEGTPEAESSPSLWDLELANQIAAVNQKPPRNGFEEMIQWTKEGKLWEFPINNEAGIEDDVEFHEHIFLDKYLEDFPKQGPIRHFMELVTCGLSKNPYLSVKQKAEHIEWFRDYFQEKEELLKEIDDHEKETDISLEKKSLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0D7</accession>
    <name>A0A452J0D7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nav/unc-53 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000033821"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038310.1">
        <property type="protein sequence ID" value="ENSGAGP00000033821.1"/>
        <property type="gene ID" value="ENSGAGG00000024067.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000316">
        <property type="entry name" value="Putative neuron navigator 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057568">
        <property type="entry name" value="CortBP2_NAV1-like_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039041">
        <property type="entry name" value="Nav/unc-53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057126">
        <property type="entry name" value="NAV1-like_ubiquitin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12784:SF18">
        <property type="entry name" value="NEURON NAVIGATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12784">
        <property type="entry name" value="STEERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25408">
        <property type="entry name" value="AAA_lid_NAV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23092">
        <property type="entry name" value="Ubiquitin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="66"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="505"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="743"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1220"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1279"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1536"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1690"/>
            <end position="1721"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1433"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="66"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="384"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="508"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="748"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="777"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="792"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="848"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="884"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="943"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1026"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1056"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1075"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1122"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1133"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1165"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1220"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1305"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1315"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1326"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1541"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1706"/>
            <end position="1721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2206" mass="236694" checksum="8339013B5A9DF094" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0R8</accession>
    <name>A0A452J0R8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ankyrin repeat and SOCS box protein 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Plays a role in spindle dynamics and genome integrity by targeting the mitotic progression protein PSRC1 for proteasomal degradation in a cell cycle-dependent manner. Also participates in meiosis by mediating the proper attachment between kinetochores and microtubules.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with CUL5. Interacts with RNF7. Interacts with PSRC1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J0R8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033961"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038459.1">
        <property type="protein sequence ID" value="ENSGAGP00000033961.1"/>
        <property type="gene ID" value="ENSGAGG00000024147.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03726">
        <property type="entry name" value="SOCS_ASB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000147">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037326">
        <property type="entry name" value="ASB7_SOCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF74">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="116"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="149"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="213"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="10">
        <location>
            <begin position="273"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="318" mass="35965" checksum="A5FD78E6803EF83C" modified="2019-05-08" version="1">MLHHHCRRNPELQEELQIQAAVAAGDVHTVRKMLEQGYSPNGRDANGWTLLHFSAARGKERCVRVFLEHGADPTVKDLIGGFTALHYAAMHGRARIARLMLESEYRSDIINAKSNDGWTPLHVAAHYGRDSFVRLLLEFKAEVDPLSDKGTTPLQLAIIRERSSCVKILLDHNANIDIQNGFLLRYAVIKSNHSYCRMFLQRGADTNLGRLEDGQTPLHLSALRDDVLCAQMLYNYGADTNTRNYEGQTPLAVSISISGSSRPCLDFLQEVTRQPRNLQDLCRIKIRQCIGLQNLKLLDELPIAKVIKDYLKHKFDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J2H2</accession>
    <name>A0A452J2H2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J2H2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034601"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039175.1">
        <property type="protein sequence ID" value="ENSGAGP00000034601.1"/>
        <property type="gene ID" value="ENSGAGG00000024543.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002090">
        <property type="entry name" value="NHE-6/7/9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF94">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01088">
        <property type="entry name" value="NAHEXCHNGR6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="12 13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="208"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="362"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="445"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="16">
        <location>
            <begin position="81"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="681"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="701" mass="76994" checksum="ABB9073AB47D710D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J3S1</accession>
    <name>A0A452J3S1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J3S1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035088"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039731.1">
        <property type="protein sequence ID" value="ENSGAGP00000035088.1"/>
        <property type="gene ID" value="ENSGAGG00000024944.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18510">
        <property type="entry name" value="BACK_KLHL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18336">
        <property type="entry name" value="BTB_POZ_KLHL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000063">
        <property type="entry name" value="Kelch-like protein 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000086">
        <property type="entry name" value="Kelch-like protein 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000027">
        <property type="entry name" value="Kelch-like protein 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF26">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00501">
        <property type="entry name" value="KELCHREPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="182"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="704" mass="78944" checksum="98A5BE9415AB5D67" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4G1</accession>
    <name>A0A452J4G1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J4G1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035320"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039994.1">
        <property type="protein sequence ID" value="ENSGAGP00000035320.1"/>
        <property type="gene ID" value="ENSGAGG00000025107.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015165">
        <property type="term" value="F:pyrimidine nucleotide-sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007271">
        <property type="entry name" value="Nuc_sug_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00803">
        <property type="entry name" value="nst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10231">
        <property type="entry name" value="NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04142">
        <property type="entry name" value="Nuc_sug_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005799">
        <property type="entry name" value="UDP-gal_transpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="339" mass="37118" checksum="B5387277372DA7F8" modified="2019-05-08" version="1">MAAPKENVSLLFKLYCLMVMTLVAATYTVALRYTRIAETELYFSTTAVCITEVIKLFLSVGILAKETGSLGRLMTSLRENVLGSPKELLKLSVPSVVYAVQNNMAFLALSNLDAAVYQVTYQLKIPCTALCTVLMLNRSLSKLQWFSVFMLCGGVTLVQWKPAQVTKVQMEQNPWLGFGAIAIAVLCSGFAGVYFEKVLKSSDTSLWVRNIQMYLSGIVVTLVGVYMSEGAQVLERGFFYGYTYYVGFVILLASVGGLYTSVVVKYTDNIMKGFSAAAAIVLSTVASVVLFGLQITVSFALGALLVCISIYLYGLPRQDTTKIQPPLETVSSKEKLIHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4Q8</accession>
    <name>A0A452J4Q8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RING/Ubox-like zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J4Q8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035445"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040133.1">
        <property type="protein sequence ID" value="ENSGAGP00000035445.1"/>
        <property type="gene ID" value="ENSGAGG00000025199.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16627">
        <property type="entry name" value="RING-HC_RBR_parkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003977">
        <property type="entry name" value="Parkin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047535">
        <property type="entry name" value="RING-HC_RBR_parkin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041170">
        <property type="entry name" value="Znf-RING_14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17978">
        <property type="entry name" value="zf-RING_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01475">
        <property type="entry name" value="PARKIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RING/Ubox-like zinc-binding" evidence="1">
        <location>
            <begin position="38"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="130" mass="14554" checksum="E0A947083E54D7C4" modified="2019-05-08" version="1">ISGENISPLPDCCRLPEFYFKCGAHPTSDSETSVALNLITTNSQHITCITCTDIRSPVLVFQCLHRHVICLDCFHLYCVTMLNDRKFIHDPVLGYSLPCVGKFLGFLNYSCPNLKKNTVDFKSSCGILCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J6L4</accession>
    <name>A0A452J6L4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Non-structural maintenance of chromosomes element 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the SMC5-SMC6 complex, that promotes sister chromatid alignment after DNA damage and facilitates double-stranded DNA breaks (DSBs) repair via homologous recombination between sister chromatids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the SMC5-SMC6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NSE4 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000036141"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040911.1">
        <property type="protein sequence ID" value="ENSGAGP00000036141.1"/>
        <property type="gene ID" value="ENSGAGG00000025612.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027786">
        <property type="entry name" value="Nse4/EID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014854">
        <property type="entry name" value="Nse4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029225">
        <property type="entry name" value="Nse4_Nse3-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16140">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16140:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15412">
        <property type="entry name" value="Nse4-Nse3_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08743">
        <property type="entry name" value="Nse4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Nse4/EID protein Nse3/MAGE-binding" evidence="12">
        <location>
            <begin position="136"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Non-structural maintenance of chromosome element 4 C-terminal" evidence="11">
        <location>
            <begin position="288"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF15412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="387" mass="43821" checksum="716720370B265D62" modified="2019-05-08" version="1">MLEAAATPNRAGARTRXGRRQPLASTSRDGAVPEHQQRRLTGGQGRQRPPETANGEGDGEEEESEEEPGQSSVRAEPEGNHTDRRLLRQQYRQLINRVQQNREDMLSSKSNSLTDALQEANKLFSGVSQAREAALDAQFLVLASNLGKEKANELHSEMTVFDSPAFAEDLLTFMGLNRLEGEENDSDDESIASGFLPNNAWHKLGAEAERYFRRSPSFHYMLGSFKADPPVPRQRIERQKRKVGTEEKRAMPSQLKKMEESHQEATEKEVERILGLLQTYFKEDPNIPISFFDFVIDPSSFARTVENIFHVSFIIRDGFARIKLDQDKLPVIEPSHEEEGRENDQNAQVRNQAVISLSHQDWKDIVETFEITEPMIPPPGVSQENES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7N9</accession>
    <name>A0A452J7N9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Myogenin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J7N9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036539"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041377.1">
        <property type="protein sequence ID" value="ENSGAGP00000036539.1"/>
        <property type="gene ID" value="ENSGAGG00000025906.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043425">
        <property type="term" value="F:bHLH transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042693">
        <property type="term" value="P:muscle cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045663">
        <property type="term" value="P:positive regulation of myoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048743">
        <property type="term" value="P:positive regulation of skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035914">
        <property type="term" value="P:skeletal muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18935">
        <property type="entry name" value="bHLH_TS_MYOG_Myf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000005">
        <property type="entry name" value="Myogenic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002546">
        <property type="entry name" value="MyoD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039704">
        <property type="entry name" value="Myogenic_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11534">
        <property type="entry name" value="MYOGENIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11534:SF5">
        <property type="entry name" value="MYOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01586">
        <property type="entry name" value="Basic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00520">
        <property type="entry name" value="BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="82"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="227" mass="25877" checksum="CCB0BA23DA6CF88E" modified="2019-05-08" version="1">MELFETSPYFFPDQRFYDGENYLSSRLQGYEQGAYQERPGLGLCPEGRTGLEEKGSPLPEHCPGQCLPWACKICKRKTVSIDRRRAATLREKRRLKKVNEAFEALKRSTLLNPNQRLPKVEILRSAIQYIERLQSLLSTLNQQEREQRDLRYRSTASQPGVASECGSNSASCSPEWSTQLEFSSHPGDHLLNDESSEDHNLHSLSSIVESIAVEDVAVTFQEERVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GG32</accession>
    <name>A0A452GG32_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GG32"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000527"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000587.1">
        <property type="protein sequence ID" value="ENSGAGP00000000527.1"/>
        <property type="gene ID" value="ENSGAGG00000000441.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031650">
        <property type="entry name" value="CCDC73"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28660">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28660:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15818">
        <property type="entry name" value="CCDC73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="104" mass="11819" checksum="B820BA8AF92EC0D6" modified="2019-05-08" version="1">MDRDFKTTTPAYTFQSSSDTLLSIHLLDFKTSLLEAVEELRVRREGSSFDLLSSQYTSAFAKLKKKANRRLVSLTYTNRFFSTISLVINIVNQGQSQTITLETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHL9</accession>
    <name>A0A452GHL9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hepcidin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the hepcidin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GHL9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001121"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001256.1">
        <property type="protein sequence ID" value="ENSGAGP00000001121.1"/>
        <property type="gene ID" value="ENSGAGG00000000911.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010500">
        <property type="entry name" value="Hepcidin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06446">
        <property type="entry name" value="Hepcidin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0372">Hormone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019500099" description="Hepcidin" evidence="6">
        <location>
            <begin position="26"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="7627" checksum="E1EF1036455D45F2" modified="2019-05-08" version="1">RNITMKLQVACVILILVITAQNSCCFQDVPAGIRALSCQAAKRHNTHFPICTFCCKCCRNQGCGFCCRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIS4</accession>
    <name>A0A452GIS4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Dynein axonemal heavy chain 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000001556"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001775.1">
        <property type="protein sequence ID" value="ENSGAGP00000001556.1"/>
        <property type="gene ID" value="ENSGAGG00000000806.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000667">
        <property type="entry name" value="Dynein axonemal heavy chain 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000219">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000002">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000001">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000002">
        <property type="entry name" value="Dynein heavy chain 9, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.130:FF:000001">
        <property type="entry name" value="Dynein, axonemal, heavy chain 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF12">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain tail" evidence="15">
        <location>
            <begin position="61"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain linker" evidence="16">
        <location>
            <begin position="1051"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="17">
        <location>
            <begin position="1590"/>
            <end position="1916"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA 5 extension" evidence="18">
        <location>
            <begin position="2074"/>
            <end position="2192"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain 3 AAA+ lid" evidence="19">
        <location>
            <begin position="2409"/>
            <end position="2505"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1017"/>
            <end position="1044"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF17857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2618" mass="304321" checksum="B7768602905914A6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJU3</accession>
    <name>A0A452GJU3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sam68 tyrosine-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GJU3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001857"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002111.1">
        <property type="protein sequence ID" value="ENSGAGP00000001857.1"/>
        <property type="gene ID" value="ENSGAGG00000001484.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032335">
        <property type="entry name" value="Sam68-YY"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16568">
        <property type="entry name" value="Sam68-YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Sam68 tyrosine-rich" evidence="3">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="140" mass="16089" checksum="85CF9DF463FD8F8F" modified="2019-05-08" version="1">MSLGGSPYLWLSKCQGLQLLEIFFKGDMKATSYYSDFAILTWSSHFYVYPYCILIIFTVFKGYDDGYGGEYDDPSYEAYDNSYAAQTQSVPEYYDYGHGTSEDAYDSYAQEEWATTRSSLKAPPPRSARGGYREHPYGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPG7</accession>
    <name>A0A452GPG7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GPG7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003801"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004399.1">
        <property type="protein sequence ID" value="ENSGAGP00000003801.1"/>
        <property type="gene ID" value="ENSGAGG00000003089.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF398">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING G-PROTEIN COUPLED RECEPTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019203375" evidence="4">
        <location>
            <begin position="23"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="167" mass="17749" checksum="37695C0C7E5E5F20" modified="2019-05-08" version="1">MLCPTSPHLALLALLSVAGAVGQGEPCPSKMNLIKDRLQVNCSGQGLSVVPLALPRNTGILLLSTNRLELILSHNALEVLPTLQGLPALTRLTLAHNNISELLPGAFRDVGKLTELNLRGNQLRTLPEEAFEGLAGLKDLDLSDNALEELPQGLLAGLELNTLWLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPL3</accession>
    <name>A0A452GPL3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPL3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003740"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004328.1">
        <property type="protein sequence ID" value="ENSGAGP00000003740.1"/>
        <property type="gene ID" value="ENSGAGG00000003036.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="89"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="302" mass="33708" checksum="504CEF395FFB9283" modified="2019-05-08" version="1">MSLPCIRCIQSNTVLFGIWIPLGTGCLDAGDRRPAELFSVKACSFGGMNCTVNHTVVTHFILLGIPNTNGLQTILFITFLAFYLCTLLGNLLIFSAILTDARLHTLMYFFLCNLSVVDLGYSSVSTPKLLANIWAQSRTISPGRCMSQVFFWHFLGSTECLLYIVMAYDWYMDICHLLHYLLIMNRRVCALLATGTWIASSLHYTILTSLTFMLPYYGSNMVEYFFCDIFPVVKLACADTYVIETVTFSNNGKVSITCFPLVLASYIRIIYSVLKMNSAEGRSKAASTCTSHLAVVTLFFGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPP1</accession>
    <name>A0A452GPP1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GPP1"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004502.1">
        <property type="protein sequence ID" value="ENSGAGP00000003878.1"/>
        <property type="gene ID" value="ENSGAGG00000003172.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019045849" description="Secreted protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="10848" checksum="04BF80E4C2073E15" modified="2019-05-08" version="1">MTMCGEMFAVTVCGLLFASLTVNKVRWECSFFPLIYTCSDEITISGAPVLGIRTEPRVPSSIVWVQGGVYMGVTRGCFPFGISAGSGGHCTAGEGLRGGCQAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPR3</accession>
    <name>A0A452GPR3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPR3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003680"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004256.1">
        <property type="protein sequence ID" value="ENSGAGP00000003680.1"/>
        <property type="gene ID" value="ENSGAGG00000002374.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006700">
        <property type="term" value="P:C21-steroid hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042359">
        <property type="term" value="P:vitamin D metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20645">
        <property type="entry name" value="CYP24A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000006">
        <property type="entry name" value="Cytochrome P450 302a1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF125">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 24 SUBFAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="518" mass="59464" checksum="E17265657BDEA540" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQ68</accession>
    <name>A0A452GQ68_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Thioredoxin reductase 1, cytoplasmic</fullName>
            <ecNumber evidence="11">1.11.1.2</ecNumber>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Peroxidase TXNRD1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Thioredoxin reductase TR1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Reduces disulfideprotein thioredoxin (Trx) to its dithiol-containing form. Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation. Contains a selenocysteine residue at the C-terminal active site that is essential for catalysis. Also has reductase activity on hydrogen peroxide (H2O2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>H2O2 + NADPH + H(+) = NADP(+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:15173"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.11.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:15174"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:20347"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.2" evidence="11"/>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452GQ68"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004057"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004735.1">
        <property type="protein sequence ID" value="ENSGAGP00000004057.1"/>
        <property type="gene ID" value="ENSGAGG00000003332.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050137">
        <property type="term" value="F:NADPH peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000190">
        <property type="entry name" value="Thioredoxin reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000004">
        <property type="entry name" value="Thioredoxin reductase 1, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006338">
        <property type="entry name" value="Thioredoxin/glutathione_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01438">
        <property type="entry name" value="TGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF8">
        <property type="entry name" value="THIOREDOXIN-DISULFIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 18" id="KW-0274">FAD</keyword>
    <keyword evidence="4 18" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10 18" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0712">Selenocysteine</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="20">
        <location>
            <begin position="70"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="22">
        <location>
            <begin position="165"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="21">
        <location>
            <begin position="522"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="649" mass="71576" checksum="56972CF363E2DEFD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSB8</accession>
    <name>A0A452GSB8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX10 also regulates PEX5 recycling by acting as a E3 ubiquitin-protein ligase. When PEX5 recycling is compromised, PEX10 catalyzes polyubiquitination of PEX5 during its passage through the retrotranslocation channel, leading to its degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452GSB8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004864"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005686.1">
        <property type="protein sequence ID" value="ENSGAGP00000004864.1"/>
        <property type="gene ID" value="ENSGAGG00000003992.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16527">
        <property type="entry name" value="RING-HC_PEX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000332">
        <property type="entry name" value="Peroxisome biogenesis factor 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025654">
        <property type="entry name" value="PEX2/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350:SF0">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="246"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="299" mass="35031" checksum="D4371B8780141DD8" modified="2019-05-08" version="1">FWNKFYIVNLTFLGTKKWLEWRKEIELLSDVAYFSLTTLSGYQTLGEEYVNIVQVDSSKKRIPSVLRRAILISLHTVLPYLLDKGLIHLEHELQTETSGFRTLQSSGLCGRTLVRSWVQKQVGRLTEQQKKTLLQTVYVLKQCIPLLRRLHLAVFYINGLFYHISKRITGISYLRVPGLPGDDQSIRSSYKLLGIVSLLHLVLTVSVQIYSFQQRQRARQEWKLHRNLSYPKNQTEEKSVVSSSRCTLCLEKRRHATATPCGHLFCWECITEWCNTKAECPLCREKFHPQKLIYLRHYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSF3</accession>
    <name>A0A452GSF3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Glucosamine 6-phosphate N-acetyltransferase</fullName>
            <ecNumber evidence="6 14">2.3.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>D-glucosamine 6-phosphate + acetyl-CoA = N-acetyl-D-glucosamine 6-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="2.3.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the acetyltransferase family. GNA1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.4" evidence="6 14"/>
    <dbReference type="AlphaFoldDB" id="A0A452GSF3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004923"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005750.1">
        <property type="protein sequence ID" value="ENSGAGP00000004923.1"/>
        <property type="gene ID" value="ENSGAGG00000004031.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00529"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000031">
        <property type="entry name" value="Glucosamine 6-phosphate N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355:SF11">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="101"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="246" mass="27066" checksum="4752897FBDE7D564" modified="2019-05-08" version="1">MKCVINRSVCNPGVPISSRDSTVVLLPSALVFPGDQHSRMMSEGSGRSTPAATIAAMMPVATVMPDDTPMFDPNLLHELDWSQNTATFSPAISPLDPGDGLVMRPLCTADLNRGFFKVLGQLTAAGVVSPEQFIKTFEHMKRSGDYYVTVVEDTNLGQIVATATLVIEHKFTHSCAKRGRVEDVVVSDECRGKQLGKLLMSTLTLLSKRLNCYKITLECLPKNVAFYKKFGYSVSEEKYMGQKFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GT89</accession>
    <name>A0A452GT89_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="43">Triacylglycerol lipase</fullName>
            <ecNumber evidence="43">3.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="43">Pancreatic lipase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="45 46">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="46" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="45" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="45" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39955"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75937"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:39956"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = di-(9Z)-octadecenoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38575"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:38576"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>1,2,3-tributanoylglycerol + H2O = dibutanoylglycerol + butanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40475"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:35020"/>
            <dbReference type="ChEBI" id="CHEBI:76478"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:40476"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>1,2,3-trioctanoylglycerol + H2O = dioctanoylglycerol + octanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:76978"/>
            <dbReference type="ChEBI" id="CHEBI:88066"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:47865"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1,2,3-tripropanoylglycerol + H2O = dipropanoylglycerol + propanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48024"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17272"/>
            <dbReference type="ChEBI" id="CHEBI:88153"/>
            <dbReference type="ChEBI" id="CHEBI:88155"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:48025"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1,2-didecanoylglycerol + H2O = decanoylglycerol + decanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48596"/>
            <dbReference type="ChEBI" id="CHEBI:11152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27689"/>
            <dbReference type="ChEBI" id="CHEBI:90605"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:48597"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1,2-didodecanoyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + H2O = dodecanoyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + dodecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48516"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18262"/>
            <dbReference type="ChEBI" id="CHEBI:90337"/>
            <dbReference type="ChEBI" id="CHEBI:90359"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:48517"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>1,2-didodecanoyl-3-beta-D-galactosyl-sn-glycerol + H2O = dodecanoyl-3-beta-D-galactosyl-sn-glycerol + dodecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48540"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18262"/>
            <dbReference type="ChEBI" id="CHEBI:90340"/>
            <dbReference type="ChEBI" id="CHEBI:90515"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:48541"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>1,2-dioctanoyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + H2O = octanoyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + octanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48692"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:90457"/>
            <dbReference type="ChEBI" id="CHEBI:90768"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:48693"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>1,2-dioctanoyl-3-O-beta-D-galactosyl-sn-glycerol + H2O = octanoyl-3-(beta-D-galactosyl)-sn-glycerol + octanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48696"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:90453"/>
            <dbReference type="ChEBI" id="CHEBI:90769"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:48697"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38487"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:38488"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1-beta-D-galactosyl-2,3-didodecanoyl-sn-glycerol + H2O = 1-beta-D-galactosyl-dodecanoyl-sn-glycerol + dodecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48536"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18262"/>
            <dbReference type="ChEBI" id="CHEBI:90342"/>
            <dbReference type="ChEBI" id="CHEBI:90514"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:48537"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + 2 H2O = 3-beta-D-galactosyl-sn-glycerol + 2 a fatty acid + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13189"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15754"/>
            <dbReference type="ChEBI" id="CHEBI:17615"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.26"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:13190"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>a 1,2-diacyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + H2O = acyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48372"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28396"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:90310"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:48373"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a monoacyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44664"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:84465"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:44665"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:12045"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>di-(9Z)-octadecenoylglycerol + H2O = (9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47868"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75937"/>
            <dbReference type="ChEBI" id="CHEBI:75945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:47869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>long chain 1,2-diacyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + H2O = long chain acyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:90463"/>
            <dbReference type="ChEBI" id="CHEBI:90774"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:48709"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>long chain 1,2-diacyl-3-O-beta-D-galactosyl-sn-glycerol + H2O = long chain acyl-3-O-beta-D-galactosyl-sn-glycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:90477"/>
            <dbReference type="ChEBI" id="CHEBI:90770"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:48701"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycerolipid metabolism; triacylglycerol degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="18">Glycolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Neuron projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 43">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Zymogen granule membrane</location>
            <topology evidence="19">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 42">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="43"/>
    <dbReference type="AlphaFoldDB" id="A0A452GT89"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005121"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005969.1">
        <property type="protein sequence ID" value="ENSGAGP00000005121.1"/>
        <property type="gene ID" value="ENSGAGG00000004123.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042589">
        <property type="term" value="C:zymogen granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047714">
        <property type="term" value="F:galactolipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004465">
        <property type="term" value="F:lipoprotein lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01759">
        <property type="entry name" value="PLAT_PL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000033">
        <property type="entry name" value="Pancreatic triacylglycerol lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000003">
        <property type="entry name" value="Triacylglycerol lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016272">
        <property type="entry name" value="Lipase_LIPH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002331">
        <property type="entry name" value="Lipase_panc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF165">
        <property type="entry name" value="PANCREATIC LIPASE-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000865">
        <property type="entry name" value="Lipoprotein_lipase_LIPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00823">
        <property type="entry name" value="PANCLIPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 39" id="KW-0106">Calcium</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13 40 41" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 43" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11 43" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 39" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="46" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 43" id="KW-0964">Secreted</keyword>
    <keyword evidence="43" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="43">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018820627" description="Triacylglycerol lipase" evidence="43">
        <location>
            <begin position="18"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="44">
        <location>
            <begin position="356"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="38">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="38">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="38">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="39">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="39">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="39">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="40">
        <location>
            <begin position="21"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="40">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="40">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="40">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="40">
        <location>
            <begin position="317"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="40 41">
        <location>
            <begin position="452"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00036575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00047744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00048386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00048546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00049076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00049154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00049290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00049352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00049420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="RuleBase" id="RU362046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="44">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="46">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="468" mass="51425" checksum="471CBA9B63C43AD0" modified="2019-05-08" version="1">MLGIWILALFLLGTARGEEVCFERLGCFTDNKPWAGTTERPVAKLPWSPETINTRFLLYTKKNPNDFQEITAINPASIGYSNFDLSKITRFITHGFLGQGEETWLSDMCKRMFEVEDVNCISVDWSGGSRCLYSQAANNIRVVGAEVAYFVNVLKEQYGYSPSKVHLIGHSLGAHAAGEAGRRSPGLGRITGLDPAQPYFQDTPVGVRLDKTDAAFVDIIHTDTAPTIPNLGFGMSQAIGHLDFYPNGGEQMPGCTNNRVSQIVDIDGIWGGTVDFVACNHLRSFKYYFESIISPNGFLGYICDSYDIFQTENCFPCPKGGCPQMGHYADKFKDRVTSGIQKLYLNTGEASDFARWRYKVSVAIAGKSKVSGYVNIALYGAGGNTRQYEIVQGTLKPGETYAKFIDVEINVGRITKVKFLWDNHVVNPTLPTLGAAAVTVQAGEDGNVFNFCGSETVQENVLQTLTSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTM3</accession>
    <name>A0A452GTM3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DDB1- and CUL4-associated factor 15 WD40 repeat-containing domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GTM3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005372"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006255.1">
        <property type="protein sequence ID" value="ENSGAGP00000005372.1"/>
        <property type="gene ID" value="ENSGAGG00000004350.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20913">
        <property type="entry name" value="DCAF15-CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20917">
        <property type="entry name" value="DCAF15-NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038914">
        <property type="entry name" value="DCAF15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047319">
        <property type="entry name" value="DCAF15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032734">
        <property type="entry name" value="DCAF15_WD40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28541">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28541:SF1">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14939">
        <property type="entry name" value="DCAF15_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDB1- and CUL4-associated factor 15 WD40 repeat-containing" evidence="2">
        <location>
            <begin position="11"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="564" mass="63446" checksum="A007A6A294B531CA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTY3</accession>
    <name>A0A452GTY3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A452GTY3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005197"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006058.1">
        <property type="protein sequence ID" value="ENSGAGP00000005197.1"/>
        <property type="gene ID" value="ENSGAGG00000004207.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18051">
        <property type="entry name" value="DEADc_DDX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000160">
        <property type="entry name" value="ATP-dependent RNA helicase DDX3X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="243"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="274"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="477"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="21"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="644"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="243"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="668"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="685"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="730" mass="80338" checksum="985507ED25D4219E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWM2</accession>
    <name>A0A452GWM2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine-rich repeat-containing protein 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the tilB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GWM2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006502"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007564.1">
        <property type="protein sequence ID" value="ENSGAGP00000006502.1"/>
        <property type="gene ID" value="ENSGAGG00000005262.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00298">
        <property type="entry name" value="ACD_sHsps_p23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000052">
        <property type="entry name" value="Leucine rich repeat containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056496">
        <property type="entry name" value="CS_DNAAF11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 410-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23602">
        <property type="entry name" value="CS_DNAAF11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CS" evidence="11">
        <location>
            <begin position="308"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="192"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="226"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="478" mass="56657" checksum="71CB709967B79CA2" modified="2019-05-08" version="1">FTSLVTEELLRQRAEHNNCELFSLEEISLHQQEIEKLEYIDKLCRDLKILYLQNNLIPKIENVSKLKKLEYLNLALNNIERIENLEGCEELQKLDLTANFIGELSSIEILKHNIHLRELFLVGNPCTEFEGYRQFVVATLHQLKCLDSKEIERSERIQALQNYRQVEKQIRDQEQVYLVKRAREKEEALKKIQEKQEKKKEKQIKPKPGFDRRWYTDINNINPDPLEDKENHQQKETEKEYQEWEIDKDDEEDRAFWEEPTPYTPEYRLEAHRHLEEKRRVKENIRGQKMKEKPPRTLITTDGKVLNMNEPKLHFFLKDDEENNQFILDLAVYRHLDSSLIDVDVQPTYLRVMVKDKPFQLVLPEEVKPDSSSAKRSQTTGHLVISMPKVQEIIRANRKTPTLVKPSDSNKLQTNVKRERVEKLEVDHSKYSFPDVANIIQEKEQTGQGPINLQQQKVIDAENSYVDFEDNQDVPPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWU7</accession>
    <name>A0A452GWU7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000006570"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007644.1">
        <property type="protein sequence ID" value="ENSGAGP00000006570.1"/>
        <property type="gene ID" value="ENSGAGG00000005317.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003109">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000012">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000189">
        <property type="entry name" value="zinc finger protein 133 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000024">
        <property type="entry name" value="zinc finger protein 140 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000759">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000812">
        <property type="entry name" value="zinc finger protein 23 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000212">
        <property type="entry name" value="zinc finger protein 382 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002004">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001532">
        <property type="entry name" value="Zinc finger protein 483"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000495">
        <property type="entry name" value="zinc finger protein 668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000188">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002208">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="422"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="450"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="491"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="547"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="575"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="603"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="631"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="659"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="685" mass="77639" checksum="9792DDA81829A64E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWU9</accession>
    <name>A0A452GWU9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GWU9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006505"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007567.1">
        <property type="protein sequence ID" value="ENSGAGP00000006505.1"/>
        <property type="gene ID" value="ENSGAGG00000005265.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000024">
        <property type="entry name" value="Serine protease 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF146">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S1" evidence="5">
        <location>
            <begin position="2"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="331" mass="36064" checksum="A3461FDC5C1974A3" modified="2019-05-08" version="1">MRLGGEGLKVRTPGSLLFPFQCCSSPGCGQPRISGRIVERGDAARGQHHCCGRSLISAQWVVSAAHCFLFSFPQSAYRVNLGEHQQSNPSPSWVSSPVRQILVHPDYSRGTHASNITLVQLAEPVRHTDEILPVCLPGPSDSVPDNHMCWVTGWRRIDSEGKTIPWGRCYHSMLQEVQVQLIDTAACNVLYSIDPASNIGRDPIKPDMMCAGERPSLIPISLFLHDDSGGPLACDHNGTWCLMGVVSWGDGCDQPNHPSVYVRTVAYGEWIWGHVVSGEQSICLVGPWCFPVCLSVCLQLVSCVLACKGWDCLNGLARWGTNLGQGDLCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GX64</accession>
    <name>A0A452GX64_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sema domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GX64"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006681"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007759.1">
        <property type="protein sequence ID" value="ENSGAGP00000006681.1"/>
        <property type="gene ID" value="ENSGAGG00000005390.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000006">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF34">
        <property type="entry name" value="PLEXIN-A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019379757" description="Sema domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="4">
        <location>
            <begin position="19"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="492" mass="55127" checksum="F2939B77F4D11FB8" modified="2019-05-08" version="1">MSAAMWKWTCLISHLLLSPMMSSLARQQPPHPKRPFATFPGEQAEGTFNHLVVDERTGHIYLGAVNRIYKLSSDLKVLVTHQTGPDEDNPKCYPPRIVQTCNEPLTLTNNINKMLLIDYKENRLIACGSLYQGICKLLRLEDLFKLGEPFHKKEHYLSGVNESGSVFGVIVSYSHMDDKLFIATAVDGKPEYFPTISSRKLTKNSEADGMFAYVFHDEFVASMIKIPSDTFTIIPDFDIYYIYGFSSGNFVYFLTLQPEMISPPGSTTKEQVYTSKLVRLCKEDAAFNSYVEVPIGCEKNGVEYRLLQAAHLSKAGAILARSLGASPDDDILFTVFSKGQKRKMKSLDESALCIFVLKKINDRIKERLQSCYRGEGTLDLAWLKVKDIPCGSALLTIDDNFCGLDMNAPLGVSEMVRGLPIFTEDGDRMTSVIAYVYKNHSLAFVGTKSGKMKKVGLVQRGFPVSAASCYGNWLHRDEWGAFYRPGAWLPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXN1</accession>
    <name>A0A452GXN1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">tRNA-dihydrouridine(47) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="19">1.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">tRNA-dihydrouridine synthase 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the synthesis of dihydrouridine, a modified base, in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). Mainly modifies the uridine in position 47 (U47) in the D-loop of most cytoplasmic tRNAs. Also able to mediate the formation of dihydrouridine in some mRNAs, thereby regulating their translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>5,6-dihydrouridine(47) in tRNA + NAD(+) = uridine(47) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53364"/>
            <dbReference type="Rhea" id="RHEA-COMP:13539"/>
            <dbReference type="Rhea" id="RHEA-COMP:13540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.89"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:53366"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>5,6-dihydrouridine(47) in tRNA + NADP(+) = uridine(47) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53360"/>
            <dbReference type="Rhea" id="RHEA-COMP:13539"/>
            <dbReference type="Rhea" id="RHEA-COMP:13540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.89"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:53362"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the dus family. Dus3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A452GXN1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006870"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007970.1">
        <property type="protein sequence ID" value="ENSGAGP00000006870.1"/>
        <property type="gene ID" value="ENSGAGG00000005527.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106414">
        <property type="term" value="F:mRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102265">
        <property type="term" value="F:tRNA-dihydrouridine47 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000067">
        <property type="entry name" value="tRNA-dihydrouridine(47) synthase [NAD(P)(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000029">
        <property type="entry name" value="tRNA-dihydrouridine(47) synthase [NAD(P)(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45846">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45846:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25585">
        <property type="entry name" value="zf-CCCH_DUS3L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 19" id="KW-0288">FMN</keyword>
    <keyword evidence="6 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="9 18 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 18 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="21">
        <location>
            <begin position="99"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="18">
        <location>
            <begin position="99"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="20"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU291113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="609" mass="69740" checksum="5C94A1F7694AA34E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXY4</accession>
    <name>A0A452GXY4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GXY4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008007.1">
        <property type="protein sequence ID" value="ENSGAGP00000006904.1"/>
        <property type="gene ID" value="ENSGAGG00000005565.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="98" mass="11262" checksum="C65B92E66FF879E3" modified="2019-05-08" version="1">MLHLFTLCFQRQLESLFTASCQRLCVIHLQGFNFHVSDDSQIYPFILDLSHSVQTSSFFLTFIIETLLSLFKTLVTSILFQAHHSDHSSLPSSLISFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GY56</accession>
    <name>A0A452GY56_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Inward rectifier potassium channel 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Inward rectifier K(+) channel Kir7.1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Potassium channel, inwardly rectifying subfamily J member 13</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ13 has a very low single channel conductance, low sensitivity to block by external barium and cesium, and no dependence of its inward rectification properties on the internal blocking particle magnesium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 20">Membrane</location>
            <topology evidence="1 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GY56"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007110"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008232.1">
        <property type="protein sequence ID" value="ENSGAGP00000007110.1"/>
        <property type="gene ID" value="ENSGAGG00000005717.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000081">
        <property type="entry name" value="inward rectifier potassium channel 13 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000004">
        <property type="entry name" value="inward rectifier potassium channel 13 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008062">
        <property type="entry name" value="KCNJ13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF3">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01679">
        <property type="entry name" value="KIR7CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 20" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 20" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 20" id="KW-0813">Transport</keyword>
    <keyword evidence="8 20" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="22">
        <location>
            <begin position="22"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="23">
        <location>
            <begin position="172"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="19">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR005465-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="362" mass="40748" checksum="0C009D260D0BEA1E" modified="2019-05-08" version="1">MIDSNNTKSSAPLLDQRHLRMVTKDGHSTLQIDGTQRKGLAYLRDAWGILMDMRWRWMMLVFAASFVIHWLVFAVLWYLLAEMNGDLELDHDAPPDNHTICVKYVTSFTAAFSFSLETQLTIGYGTMFPSGDCPSAIALLAIQMVLGLMLEAFITGAFVAKIARPKNRAFSIRFTHSAVVAHTEGKPYLMFQVANTRPSPLTSVRVSAILYQEQENGQLHQTSVDFHLDSITFEECPFFIFPLTYHHSITPSSPLGVLLQGDTPRHFELVVFLSAMQEGTGETCQRRTSYLPSEIILHHRFASMLARGAKGEYQIKMENFDKTIPELPAAADSKSPKRTDMKIRINGQHIDSFQLSETGLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYT0</accession>
    <name>A0A452GYT0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GYT0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007412"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008561.1">
        <property type="protein sequence ID" value="ENSGAGP00000007412.1"/>
        <property type="gene ID" value="ENSGAGG00000005951.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd09941">
        <property type="entry name" value="SH2_Grb2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11949">
        <property type="entry name" value="SH3_GRB2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11946">
        <property type="entry name" value="SH3_GRB2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000067">
        <property type="entry name" value="Growth factor receptor-bound protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000076">
        <property type="entry name" value="Growth factor receptor-bound protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000022">
        <property type="entry name" value="Growth factor receptor-bound protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043539">
        <property type="entry name" value="Grb2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035643">
        <property type="entry name" value="GRB2_C_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035641">
        <property type="entry name" value="GRB2_N_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46037">
        <property type="entry name" value="PROTEIN ENHANCER OF SEVENLESS 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 12" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="5 13" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="14">
        <location>
            <begin position="60"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="156"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="217" mass="25163" checksum="203BA3441A56368E" modified="2019-05-08" version="1">MEAIAKYDFKATADDELSFKRGDVLKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLGKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEEGELGFRRGDFIQVLDNSDPNWWKGACHGQTGMFPRNYVTPVNRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZM9</accession>
    <name>A0A452GZM9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GZM9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007536"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008701.1">
        <property type="protein sequence ID" value="ENSGAGP00000007536.1"/>
        <property type="gene ID" value="ENSGAGG00000006029.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01345">
        <property type="entry name" value="CERVTRCPTASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="333" mass="38629" checksum="5662DEC3E562E0EF" modified="2019-05-08" version="1">MGMNMKVVSWIRNWLKGRLQRVVLKGELSGWKEVTSGVPQGSVLGPILFNLFITDLGTKSGNVLIKFADDTKLGGIANTEKDRDTIQEDLNHLVNWSNRNRMKYNSEKCKVMHLGINNKNFGYMLGAHQLEATEEEKDLGVLVDSRMTMSRQCDTAVKKANAILGCIRRGISCKDKEVLVPLYKALVRPHLEYCVQFWCPMFKKDEFKLEQVQRRATRMIRGMEKLPYERRLKELGLFSLAKRRLRGDMLALYKYIRGVNVREGEELFKFSTNVGTRTNGYKLDIRKFRLEIRRRFLTIRGVKFWNSLPREVVGAKDFPGFKTKLDKYMEGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1L8</accession>
    <name>A0A452H1L8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H1L8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008438"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009714.1">
        <property type="protein sequence ID" value="ENSGAGP00000008438.1"/>
        <property type="gene ID" value="ENSGAGG00000006709.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11516" checksum="02DFEA57A7C386A6" modified="2019-05-08" version="1">MVGGNCTEVTEFILKGFTDHPDIQVLLFMVFLVIYIITLLGNLGMIMIDPRLHTPMYYFLGNLAFVDLCYATVIAPKMLVDLLAERKSASYPVTMTLRDLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H265</accession>
    <name>A0A452H265_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Beta-1,4-galactosyltransferase</fullName>
            <shortName evidence="17">Beta-1,4-GalTase</shortName>
            <ecNumber evidence="17">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N-acetyl-D-glucosamine + UDP-alpha-D-galactose = beta-D-galactosyl-(1-&gt;4)-N-acetyl-D-glucosamine + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17745"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:60152"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:506227"/>
            <dbReference type="EC" id="2.4.1.90"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:17746"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Golgi apparatus membrane</location>
            <topology evidence="2 17">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A452H265"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008496"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009781.1">
        <property type="protein sequence ID" value="ENSGAGP00000008496.1"/>
        <property type="gene ID" value="ENSGAGG00000006754.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000009783.1">
        <property type="protein sequence ID" value="ENSGAGP00000008498.1"/>
        <property type="gene ID" value="ENSGAGG00000006754.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003945">
        <property type="term" value="F:N-acetyllactosamine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00899">
        <property type="entry name" value="b4GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000028">
        <property type="entry name" value="beta-1,4-galactosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF9">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14 17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 17" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="9 17" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604483" description="Beta-1,4-galactosyltransferase" evidence="18">
        <location>
            <begin position="31"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="20">
        <location>
            <begin position="83"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="19">
        <location>
            <begin position="219"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="353" mass="40939" checksum="13B8D09113EAB115" modified="2019-05-08" version="1">MGLSSVIFHLSYKFRLLLLFSLCMMVVAWATSSYLVDIMQETPKAKSLVENFRKATTLEKENQKKKAMTTVEVHTVKFTMSHCPALSPYLQGPSKLKFKASLTLEEIQKENPQVAKGRHRPAECSALQRVAILIPHRNREKHLLYLLEHLHPFLQRQQLDYGIYVIHQAGNTKFNRAKLLNVGYLEAVKEENWDCFIFHDVDLVPENDLNMYTCDNQPKHLVVGRNNTGYRLRYQGYFGGVTALTRDQFSKVNGFSNNYWGWGGEDDDLRIRVEMQKMRVMRPSPDVGKYTMIFHTRDRGNEENGERMKLLRQVSRVWKTDGLNSCSYKLLSVEHNPLYINVTVDFSMPPKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2S3</accession>
    <name>A0A452H2S3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain containing 13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H2S3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008710"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010007.1">
        <property type="protein sequence ID" value="ENSGAGP00000008710.1"/>
        <property type="gene ID" value="ENSGAGG00000006889.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038929">
        <property type="entry name" value="CCDC13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31935">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31935:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="405"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="517"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="611"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="137"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="311"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="431"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="414"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="616"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="719" mass="81146" checksum="617CAE10B90D81F7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3D0</accession>
    <name>A0A452H3D0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the chemokine-like receptor (CMKLR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3D0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009130"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010484.1">
        <property type="protein sequence ID" value="ENSGAGP00000009130.1"/>
        <property type="gene ID" value="ENSGAGG00000007211.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004875">
        <property type="term" value="F:complement receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004982">
        <property type="term" value="F:N-formyl peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000034">
        <property type="entry name" value="G-protein coupled receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000826">
        <property type="entry name" value="Formyl_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225">
        <property type="entry name" value="CHEMOTACTIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225:SF0">
        <property type="entry name" value="N-FORMYL PEPTIDE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00526">
        <property type="entry name" value="FMETLEUPHER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="46"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="351" mass="39290" checksum="89150144259E57BE" modified="2019-05-08" version="1">AEEVASCPLSQTHRLRPLLAPCTQREWIGVLFAILFSLGFLLGVIGNGLVIFIASFRMTKTVVSIWYLNLAVADFIFTSFLSIEVAYAALNFHWPFGRTLCKIHSAVSFLNLFSSVFLLTVISVDRCISVAWPVWAQNHRSPRLAFWVAVAVWLVALALSSPYIIFRDIRSFPLQGDITLCYNNYIPMGNFTSQEVTMVWKHRHQAMVLTSFVTGFLVPFVIILTCYGVMVAKLTRNHLVHSGRPYKIMVAVVTAFFLCWFPYHLSTLLAMSAEGVTPAIQVLLDVALPLAYLNSCLNPVLYAFMGWGCKDTLWHSVVLAFQRAFGEAEAQANLPSKSQDSSRLEAELPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H459</accession>
    <name>A0A452H459_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SMG7 nonsense mediated mRNA decay factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H459"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009406"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010808.1">
        <property type="protein sequence ID" value="ENSGAGP00000009406.1"/>
        <property type="gene ID" value="ENSGAGG00000006973.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045153">
        <property type="entry name" value="Est1/Ebs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696:SF5">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY FACTOR SMG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696">
        <property type="entry name" value="SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="681"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="799"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="681"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="692"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="729"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="757"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="811"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="831" mass="91915" checksum="EC0DEEBECD0B5482" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4I6</accession>
    <name>A0A452H4I6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the round spermatid basic protein 1 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000009551"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010979.1">
        <property type="protein sequence ID" value="ENSGAGP00000009551.1"/>
        <property type="gene ID" value="ENSGAGG00000007540.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354:SF9">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE RSBN1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="617" mass="69278" checksum="5EFBE9A7CE52BB79" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6G9</accession>
    <name>A0A452H6G9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BicD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H6G9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010299"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011824.1">
        <property type="protein sequence ID" value="ENSGAGP00000010299.1"/>
        <property type="gene ID" value="ENSGAGG00000008066.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008093">
        <property type="term" value="F:cytoskeletal anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034452">
        <property type="term" value="F:dynactin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072393">
        <property type="term" value="P:microtubule anchoring at microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018477">
        <property type="entry name" value="BICD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31233">
        <property type="entry name" value="BICAUDAL D FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31233:SF7">
        <property type="entry name" value="PROTEIN BICAUDAL D HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="47"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="92" mass="10552" checksum="B47B66E13C249258" modified="2019-05-08" version="1">MALATEEEEYAKLVMESEPEWLRSELKRLSQELGETTREKIQAAEYGLAVLEEKQQLKQQYDELEAEYETIRTEMEQLKEVSRGAGAVAGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6K2</accession>
    <name>A0A452H6K2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the p23/wos2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H6K2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010334"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011864.1">
        <property type="protein sequence ID" value="ENSGAGP00000010334.1"/>
        <property type="gene ID" value="ENSGAGG00000008101.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00237">
        <property type="entry name" value="p23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000003">
        <property type="entry name" value="prostaglandin E synthase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045250">
        <property type="entry name" value="p23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932:SF4">
        <property type="entry name" value="PROTEIN PTGES3L-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN P23 HSP90 CO-CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="2">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="211" mass="24107" checksum="5C91D1F2373341B3" modified="2019-05-08" version="1">MARQSAKTLWYDRPWYIFLEFCVEDSTDVKVDIEDHRVVFSCKNADGVEMYNEINLYARVNSKDSQQKRSDRSITCFVRKWKEKVAWPRLTKENIKPVWLFVDFDNWRDWEGDEEAEMAMTEQYAELLQKVTVKGTPPAMDDLDVSTVGHCLAETVSSGGFTQPGSLPLALPVPKPGSQLLHLCQPMGSLLSAWTKAKLLPGQRFSEIPLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H787</accession>
    <name>A0A452H787_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coagulation factor VII</fullName>
            <ecNumber evidence="3">3.4.21.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Serum prothrombin conversion accelerator</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa also converts factor IX to factor IXa in the presence of tissue factor and calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.</text>
            <dbReference type="EC" id="3.4.21.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.21" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452H787"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010557"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012119.1">
        <property type="protein sequence ID" value="ENSGAGP00000010557.1"/>
        <property type="gene ID" value="ENSGAGG00000008239.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000259">
        <property type="entry name" value="Coagulation factor VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000420">
        <property type="entry name" value="Coagulation factor VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000013">
        <property type="entry name" value="Coagulation factor X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012224">
        <property type="entry name" value="Pept_S1A_FX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF26">
        <property type="entry name" value="COAGULATION FACTOR VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001143">
        <property type="entry name" value="Factor_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="18 23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 23" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="13 24" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 24" id="KW-0645">Protease</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="14 24" id="KW-0720">Serine protease</keyword>
    <keyword evidence="11 25" id="KW-0732">Signal</keyword>
    <keyword evidence="17" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019230060" description="Coagulation factor VII" evidence="25">
        <location>
            <begin position="23"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Gla" evidence="28">
        <location>
            <begin position="56"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="26">
        <location>
            <begin position="104"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="27">
        <location>
            <begin position="210"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="22">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="22">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="22">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00056668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR001143-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="442" mass="49298" checksum="46BAC6F6092A2BB3" modified="2019-05-08" version="1">MVSRHYLVLLLCFLLLLPLLLAAGIEKMCMQSSNYFNFSVFLKQDQANSLLKRQRRANSLFEELKIGSLQRECLEEKCSYEEAREIFQDDKRTVTLLLLCVFPDANQCDSDPCQNGGTCVDQFQAYVCLCPEEYKGKNCEKGLNDKPRCINENGYCEHYCIDSPTMIRQCFCAEGYVLASDEVSCTPQVDYPCGKIPVLTKQSASPQGRIVGGYVCPPGECPWQALLIENEKAKCGGSLLGPSWVVTAAHCLEYTHPNQLRVKLGTIDHEDEGEQERKVSEIIMHEKYIPQKTDHDIALLRLEAPVNFSDYVVPICLPEKQFATSELSSIRFSTVSGWGRLIEGGAAAAVLMRVDLPRVKTQECIQKANLNITENMFCAGDLSGTKDSCKGDSGGPHATNYKNTSFLTGIVSWGKGCATEGTYGVYTRVSKYLEWLNNHMDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7Y8</accession>
    <name>A0A452H7Y8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Synaptotagmin-like protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H7Y8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010872"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012453.1">
        <property type="protein sequence ID" value="ENSGAGP00000010872.1"/>
        <property type="gene ID" value="ENSGAGG00000008450.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042043">
        <property type="term" value="F:neurexin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08393">
        <property type="entry name" value="C2A_SLP-1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04020">
        <property type="entry name" value="C2B_SLP_1-2-3-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000006">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000040">
        <property type="entry name" value="synaptotagmin-like protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043567">
        <property type="entry name" value="SYTL1-5_C2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716">
        <property type="entry name" value="BITESIZE, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716:SF8">
        <property type="entry name" value="SYNAPTOTAGMIN LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RabBD" evidence="9">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="666"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="803"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="78"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="316"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="403"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="510"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="149"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="341"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="410"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="518"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="969" mass="108681" checksum="AC2E871761D50202" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8B2</accession>
    <name>A0A452H8B2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lysophospholipase</fullName>
            <ecNumber evidence="3">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28610"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:40808"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-hexadecanoyl-sn-glycero-3-phosphate + H2O = sn-glycerol 3-phosphate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49092"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57518"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:49093"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40435"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:40436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:15178"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type III membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NTE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010959"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012553.1">
        <property type="protein sequence ID" value="ENSGAGP00000010959.1"/>
        <property type="gene ID" value="ENSGAGG00000008501.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd07225">
        <property type="entry name" value="Pat_PNPLA6_PNPLA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000010">
        <property type="entry name" value="neuropathy target esterase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000012">
        <property type="entry name" value="neuropathy target esterase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000001">
        <property type="entry name" value="neuropathy target esterase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000022">
        <property type="entry name" value="Patatin like phospholipase domain containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF23">
        <property type="entry name" value="PATATIN-LIKE PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 17" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11 17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="20">
        <location>
            <begin position="202"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="20">
        <location>
            <begin position="533"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="20">
        <location>
            <begin position="642"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="21">
        <location>
            <begin position="976"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="365"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1331"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="17">
        <location>
            <begin position="980"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="17">
        <location>
            <begin position="1007"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="17">
        <location>
            <begin position="1129"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="365"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="402"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1332"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="17">
        <location>
            <position position="1009"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="1129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1364" mass="152615" checksum="5CBA84A0ADE83BF2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HC95</accession>
    <name>A0A452HC95_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSGAGT00000014186.1">
        <property type="protein sequence ID" value="ENSGAGP00000012395.1"/>
        <property type="gene ID" value="ENSGAGG00000009518.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="34"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="365" mass="41233" checksum="DBBEE2C378936393" modified="2019-05-08" version="1">MGKDVAKYVRSCEVCQRVGKPQDQVKAPLQPLPIIEVPFQRVAVDILGPFPKKTPRGKQYVLTLVDFATRWPEAVALGNTRANTVCLALTDIFARVGWPSDILTDSGSNFLAGTMEKLWETHGVNHLVATPYHHQTNGLVERFNGTLGAMIRKFINEFSNNWDLVLQQLLFAYRAVPHPSLGFSPFELVYGHEVKGPLQLVKQQWEGFTPSPGTNILDFVSNLQSTLRHSLALARENLKDAQEEQKAWYDRHARERSFKVGDQVMVLKAQQAHKMEASWEGPFTVQERLGAVNYLIAFPNSSLKPKVYHVNSLKPFYSRDLQVCQFTVQGDDAEWPDGVYYDGKKDGGVEEVNLSTTLERLQRXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCS1</accession>
    <name>A0A452HCS1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">STI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HCS1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012587"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014428.1">
        <property type="protein sequence ID" value="ENSGAGP00000012587.1"/>
        <property type="gene ID" value="ENSGAGG00000009669.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14438">
        <property type="entry name" value="Hip_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000007">
        <property type="entry name" value="hsc70-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000080">
        <property type="entry name" value="hsc70-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3420:FF:000001">
        <property type="entry name" value="Hsc70-interacting protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034649">
        <property type="entry name" value="Hip_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041243">
        <property type="entry name" value="STI1/HOP_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45883">
        <property type="entry name" value="HSC70-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45883:SF2">
        <property type="entry name" value="HSC70-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18253">
        <property type="entry name" value="HipN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17830">
        <property type="entry name" value="STI1-HOP_DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="111"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="145"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="9">
        <location>
            <begin position="302"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="38"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="71"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="352" mass="39608" checksum="EFCAC3A509428C0E" modified="2019-05-08" version="1">MDPRKVSELRAFVKLCKESPGLLHSEELRFLREWVESMGGTIPPAPHNASAETVKDKTEKQPEEPVKPPEPESEESDLEIDNEGVIEPDKDDPQEMGDENVEVTEEMMDEADEKKMEGIHALGEGELQKAINLFTEAIKLNPHLAILYAKRASVFVKLQKPNAAIRDCDRAIQINPDSAQTYKWRGKAHRLLGHWEEAAHDLALACKLDYDEEASAMLKEVQPRAQKIAEHRRKYERKREEKEIKERLERVKKAREEHERAQREEEARQQAGGAQFGGFPGGFPGGMPGMAGMPGLNEILSDPEVLAAMQDPEVMVAFQDVAQNPANMSKYQNNPKVMNLISKLSAKFGSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCV6</accession>
    <name>A0A452HCV6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HCV6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012629"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014481.1">
        <property type="protein sequence ID" value="ENSGAGP00000012629.1"/>
        <property type="gene ID" value="ENSGAGG00000009698.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016037">
        <property type="term" value="P:light absorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15336">
        <property type="entry name" value="7tmA_Melanopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000083">
        <property type="entry name" value="Melanopsin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 14" id="KW-0157">Chromophore</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 14" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="12 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="4 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="44"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="406" mass="45638" checksum="A1EBB8C3E670536F" modified="2019-05-08" version="1">CRLGPAEMTVQDVPHAFPTLDVPDHAHYTIGTVILVVGITGTLGNFMVIYAFCRSRSLRTPANMFIINLAISDFLMSITQAPIFFTTSLHKHWIFGKKGCELYAFCGALFGITSMITLMAIALDRYFVITRPLASIVVMSKKKALIILLGVWLYSLAWSLPPFFGWSAYVPEGLLTSCSWDYVTFTPSVRAYTMLLFCFVFFIPLIAIIYSYVFIFKAIKNTNEAVQNIGSDANKVSQRQYQKMKNEWKMAKIALIIILLYVISWSPYSVVALVAFAGYSHLLTPFMNTVPAVIAKASAIHNPIIYAITHPKYRMAIAKYVPCLRPLLRVSRKDSRSCSRYLSTRRSTIISQSEISGLQKGKRRQSSLSDSESVRKTLQISSFKVVGRLDEKMHYGARYKGSLRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDG9</accession>
    <name>A0A452HDG9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FIIND domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HDG9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012847"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014722.1">
        <property type="protein sequence ID" value="ENSGAGP00000012847.1"/>
        <property type="gene ID" value="ENSGAGG00000009855.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061702">
        <property type="term" value="C:canonical inflammasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025307">
        <property type="entry name" value="FIIND_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051249">
        <property type="entry name" value="NLRP_Inflammasome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46985:SF4">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46985">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13553">
        <property type="entry name" value="FIIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51830">
        <property type="entry name" value="FIIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FIIND" evidence="5">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="201" mass="22494" checksum="D0EFE6A1E3FFEF65" modified="2019-05-08" version="1">TPEVATFQEIHPLVLNSFLPALCRVHFPRAGSFRCSETELGLEVRAAVTVHYGYDSWDQHLSASEKQQWMVAGPLFNIRVVEPAEAGAVAAVHLPHFLCLSAEADVSQLQVAHFVDCGMTLESPTRRRPFHAVLENPSFSPIGLLWKQICSTLFPPVHSLVLLYRSRRAADITLHFYLLPRDLSLVHQWLSAMNLSSSLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDP7</accession>
    <name>A0A452HDP7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">F-box only protein 43</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HDP7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012935"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014823.1">
        <property type="protein sequence ID" value="ENSGAGP00000012935.1"/>
        <property type="gene ID" value="ENSGAGG00000009924.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045835">
        <property type="term" value="P:negative regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007088">
        <property type="term" value="P:regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20365">
        <property type="entry name" value="BRcat_RBR_FBXO43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22171">
        <property type="entry name" value="F-box_FBXO43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000006">
        <property type="entry name" value="F-box only protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000046">
        <property type="entry name" value="F-box protein 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047147">
        <property type="entry name" value="FBX5_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044064">
        <property type="entry name" value="ZF_ZBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15493:SF1">
        <property type="entry name" value="F-BOX ONLY PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15493">
        <property type="entry name" value="F-BOX ONLY PROTEIN 5 AND 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51872">
        <property type="entry name" value="ZF_ZBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZBR-type" evidence="12">
        <location>
            <begin position="640"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="684"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="712" mass="79703" checksum="38BEFC42AAC1F9B0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEC3</accession>
    <name>A0A452HEC3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HEC3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013171"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015079.1">
        <property type="protein sequence ID" value="ENSGAGP00000013171.1"/>
        <property type="gene ID" value="ENSGAGG00000010091.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="292" mass="33249" checksum="AF96DD03894E775B" modified="2019-05-08" version="1">MEQVIKEIICKHLEGGKVIGNSQHGFVKNKSCQTNLIVFFDRITDLVDKGEAVDVIYLDFSKAFDTVSHDILIDKLGKYNLDGATIRWVHNWLDNRTQRVVVNGSQSCWKGITSGVPQGSVLGPVLFNIFINDLDVGIESTLIKFADDTKLGGIATALEDRVKIQNDLDKLEKWSEVNRMKFNKDKCKVLHLGRNNQFHTYRMGRDCLGRSMAERDLGVIVDHKLNMSEQCDTVAKKANVILGCINRCVVNKTREVILPLYSALVRPQLEYCVQFWAPHFKKDVEKLERVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFX3</accession>
    <name>A0A452HFX3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Peptidase M12B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000013706"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015699.1">
        <property type="protein sequence ID" value="ENSGAGP00000013706.1"/>
        <property type="gene ID" value="ENSGAGG00000010449.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000003">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF20">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="8 13 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="17">
        <location>
            <begin position="161"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1311"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="active site" evidence="12 15">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="169"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="273"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="284"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1331" mass="146164" checksum="F221628CE93C8BCB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGJ5</accession>
    <name>A0A452HGJ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Diacylglycerol kinase</fullName>
            <shortName evidence="21">DAG kinase</shortName>
            <ecNumber evidence="21">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:40328"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:10273"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="20">Glycerolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; glycerolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="21"/>
    <dbReference type="AlphaFoldDB" id="A0A452HGJ5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013989"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016010.1">
        <property type="protein sequence ID" value="ENSGAGP00000013989.1"/>
        <property type="gene ID" value="ENSGAGG00000010652.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000016015.1">
        <property type="protein sequence ID" value="ENSGAGP00000013994.1"/>
        <property type="gene ID" value="ENSGAGG00000010652.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00230"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20894">
        <property type="entry name" value="C1_DGKeta_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13274">
        <property type="entry name" value="PH_DGK_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09507">
        <property type="entry name" value="SAM_DGK-delta-eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000021">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000060">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000001">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000002">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000029">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000001">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047480">
        <property type="entry name" value="C1_DGKeta_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054474">
        <property type="entry name" value="DGKD_4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF33">
        <property type="entry name" value="DIACYLGLYCEROL KINASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22944">
        <property type="entry name" value="DGKD_4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="24">
        <location>
            <begin position="67"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="25">
        <location>
            <begin position="177"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="25">
        <location>
            <begin position="249"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="DAGKc" evidence="27">
        <location>
            <begin position="331"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="26">
        <location>
            <begin position="1092"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="17"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="574"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="544"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="609"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="937"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00060536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1156" mass="129087" checksum="C8463F45E7CECFF7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHE5</accession>
    <name>A0A452HHE5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HHE5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014301"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016362.1">
        <property type="protein sequence ID" value="ENSGAGP00000014301.1"/>
        <property type="gene ID" value="ENSGAGG00000010873.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19494">
        <property type="entry name" value="Elp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000623">
        <property type="entry name" value="Elongator acetyltransferase complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008728">
        <property type="entry name" value="Elongator_complex_protein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896">
        <property type="entry name" value="PAX6 NEIGHBOR PROTEIN PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05625">
        <property type="entry name" value="PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="374" mass="42234" checksum="FD620D473F130558" modified="2019-05-08" version="1">MAALATGGGMPSDGATSFQRKVPASSRLATIAGTRPSVRHGQLLLSSGLPSLDCVLEEDKYSIYSNLLFKYFLAEGIVCGHNLFVASAKEDPADILKELPAPLFDDAHKNQVNEKETAIKSKQESQESMKIAWRYQNLPRIEVSPAASARIGHYYDVSKMVSPELLQSTKWHRFFVPEEMSLHPEIKTCNMTCGYTRLLQSIQRIIYQEGFDGSYPQKKQKNILRIGIQSLGSVLWGDDICCTDNPEDIYSLTKFLYVLRGLLRMSLSACIITVPAHLIQNKAIMERVTNLSDTVVGLESFIGSERETNPLYKDYQGLIHVHKIPRLNSLICDVSGTKDLAFRLKRKLFTIEVRETVLFHYLEPERWSKSYHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHP5</accession>
    <name>A0A452HHP5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fanconi Anaemia group E protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HHP5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014413"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016485.1">
        <property type="protein sequence ID" value="ENSGAGP00000014413.1"/>
        <property type="gene ID" value="ENSGAGG00000010953.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07439">
        <property type="entry name" value="FANCE_c-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039685">
        <property type="entry name" value="FANCE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021025">
        <property type="entry name" value="Fanconi_anaemia_gr_E_prot_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32094">
        <property type="entry name" value="FANCONI ANEMIA GROUP E PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32094:SF5">
        <property type="entry name" value="FANCONI ANEMIA GROUP E PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11510">
        <property type="entry name" value="FA_FANCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fanconi Anaemia group E protein C-terminal" evidence="2">
        <location>
            <begin position="304"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="558" mass="62520" checksum="EF29C79D4229E13E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJN6</accession>
    <name>A0A452HJN6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HJN6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015127"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017281.1">
        <property type="protein sequence ID" value="ENSGAGP00000015127.1"/>
        <property type="gene ID" value="ENSGAGG00000011417.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF156">
        <property type="entry name" value="KRAB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="22"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="102" mass="11067" checksum="DE6DE2B8B3ABC945" modified="2019-05-08" version="1">ASLCFPLPVSAGGGRLFFQVPVTFADVAVHFTAAEWALLGDSQRELYRDTMLENYGNMASLGIPIHKPDIISCLERGEEPFVPAAQGLEEPEILRGIYTGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJV0</accession>
    <name>A0A452HJV0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000015191"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017349.1">
        <property type="protein sequence ID" value="ENSGAGP00000015191.1"/>
        <property type="gene ID" value="ENSGAGG00000011467.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21246">
        <property type="entry name" value="CH_SPTB-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000003">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000004">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000172">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="119"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="238"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="513"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="546" mass="61937" checksum="4C80C9E71B85C76C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJY6</accession>
    <name>A0A452HJY6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'(3')-deoxyribonucleotidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJY6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015205"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017366.1">
        <property type="protein sequence ID" value="ENSGAGP00000015205.1"/>
        <property type="gene ID" value="ENSGAGG00000011446.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000026">
        <property type="entry name" value="NT5DC3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008380">
        <property type="entry name" value="HAD-SF_hydro_IG_5-nucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016695">
        <property type="entry name" value="Pur_nucleotidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103:SF11">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05761">
        <property type="entry name" value="5_nucleotid"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017434">
        <property type="entry name" value="Purine_5'-nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR017434-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR017434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="468" mass="54505" checksum="C2BA8431BDE29E15" modified="2019-05-08" version="1">MTSSPSLPPPPPPGAAGTCSPIPGAMRGQGRQGPCLSPAANLLNPDAIFTNNEMSLSDIEIYGFDYDYTLVFYSKHLHTLIYNAARDLLINEHRYPAEIRKYDYDPNFAIRGLHYDVHRALLMKIDAFHYIQLGTVYRGFSVVPDEEVIAMYDGSHVPLEQMSDFYGKDSIRDVHIKGIMYRAIEADIEKYVCYAEQTRAVLRKLADHGKKIFLITNSPSSFVDKGMKFLVGKDWRDLFDVVIVQADKPNFFNDKRRPFRKVNERGVLLWDKIHKLQKGQIYKQGNLYEFLKLTGWRGSKVLYFGDHIYSDLADLTLKHGWRTGAIIPELRSEIKIMNTEQYIQTMTWLQTLTGLLEHMQIYQDPDSQMILQEWKKERKEMREMTRNFFNSQFGSLFRTDQNPTYFLRRLSRFADIYMASLSCLLNYDLNYTFYPRRTPLQHELPTWSDQLCSGAFRIPLLQEMAQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKC8</accession>
    <name>A0A452HKC8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GAB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HKC8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015374"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017558.1">
        <property type="protein sequence ID" value="ENSGAGP00000015374.1"/>
        <property type="gene ID" value="ENSGAGG00000011596.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13385">
        <property type="entry name" value="PH_Gab3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046355">
        <property type="entry name" value="Gab1-4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45960">
        <property type="entry name" value="GRB2-ASSOCIATED-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45960:SF3">
        <property type="entry name" value="GRB2-ASSOCIATED-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="5"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="436"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="598" mass="66613" checksum="612FAC7E20D55A66" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQ15</accession>
    <name>A0A452HQ15_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Diacylglycerol kinase</fullName>
            <shortName evidence="27">DAG kinase</shortName>
            <ecNumber evidence="27">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="25">Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle. Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1,2-di-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycerol + ATP = 1,2-di-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40351"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77125"/>
            <dbReference type="ChEBI" id="CHEBI:77126"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:40352"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycerol + ATP = 1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40355"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77127"/>
            <dbReference type="ChEBI" id="CHEBI:77128"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:40356"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:40328"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1-eicosanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycerol + ATP = 1-eicosanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40331"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77094"/>
            <dbReference type="ChEBI" id="CHEBI:87223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:40332"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40335"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:72864"/>
            <dbReference type="ChEBI" id="CHEBI:77096"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:40336"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1-octadecanoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycerol + ATP = 1-octadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40339"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77097"/>
            <dbReference type="ChEBI" id="CHEBI:77098"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:40340"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:10273"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="26">Glycerolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; glycerolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 27">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="27"/>
    <dbReference type="AlphaFoldDB" id="A0A452HQ15"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017115"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019534.1">
        <property type="protein sequence ID" value="ENSGAGP00000017115.1"/>
        <property type="gene ID" value="ENSGAGG00000012787.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00230"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20801">
        <property type="entry name" value="C1_DGKepsilon_typeIII_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20853">
        <property type="entry name" value="C1_DGKepsilon_typeIII_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000005">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000002">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000007">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF118">
        <property type="entry name" value="DIACYLGLYCEROL KINASE EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 27" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 27" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="30">
        <location>
            <begin position="57"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="30">
        <location>
            <begin position="122"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="DAGKc" evidence="31">
        <location>
            <begin position="213"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="557"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00050690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00050897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00051725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00052317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00053625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00060536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="569" mass="64360" checksum="B40D852CEA707031" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQE7</accession>
    <name>A0A452HQE7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1</fullName>
            <ecNumber evidence="5">2.4.99.20</ecNumber>
            <ecNumber evidence="4">3.2.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">2'-phospho-ADP-ribosyl cyclase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">2'-phospho-cyclic-ADP-ribose transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">ADP-ribosyl cyclase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Cyclic ADP-ribose hydrolase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADP-ribosyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.20" evidence="5"/>
    <dbReference type="EC" id="3.2.2.6" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452HQE7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017256"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019684.1">
        <property type="protein sequence ID" value="ENSGAGP00000017256.1"/>
        <property type="gene ID" value="ENSGAGG00000012850.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016849">
        <property type="term" value="F:phosphorus-oxygen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030890">
        <property type="term" value="P:positive regulation of B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04759">
        <property type="entry name" value="Rib_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.82.10">
        <property type="entry name" value="ADP Ribosyl Cyclase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003193">
        <property type="entry name" value="ADP-ribosyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912:SF5">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE_CYCLIC ADP-RIBOSE HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02267">
        <property type="entry name" value="Rib_hydrolayse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52309">
        <property type="entry name" value="N-(deoxy)ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00030418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00031355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="289" mass="32665" checksum="9E4FA7DF5C8E9BCF" modified="2019-05-08" version="1">MPLQNRSSKTRRRTILLVIIMVLIVTIVAVLAAVLLANETKETSTQLLQWKGRGTTKNLQEVILGRCYNYITTVNPELRDKDCQNIWETMQKAFIYKNPCNITTEDYQPLLDLTSHPIPCNKSLFWSKTNDLVHRYTKTSHNFLTLEDTLLGYMADGVSWCGNPSSPGINYESCPKWSECESNPSSVYWKMASKMFAEAACGVVQVMLNGSIEAGAFRSNSIFGSIEIFNLDPDKVSTVQIWLMQNIGGPQNCFLSVNYMQLQSYLHNPVIFKFCTSLCLCNPTEAQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQH4</accession>
    <name>A0A452HQH4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000017259"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019687.1">
        <property type="protein sequence ID" value="ENSGAGP00000017259.1"/>
        <property type="gene ID" value="ENSGAGG00000011343.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016525">
        <property type="term" value="P:negative regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15988">
        <property type="entry name" value="7tmB2_BAI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000016">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.610:FF:000002">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000003">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000004">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000012">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000002">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032471">
        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051867">
        <property type="entry name" value="Angio_Inhib/Adhesion_GPCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008077">
        <property type="entry name" value="GPCR_2_brain_angio_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10239:SF32">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10239">
        <property type="entry name" value="ISTHMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16489">
        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01694">
        <property type="entry name" value="BAIPRECURSOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0807">Transducer</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="717"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="750"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="788"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="821"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="859"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="932"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="959"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="19">
        <location>
            <begin position="322"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="18">
        <location>
            <begin position="565"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="20">
        <location>
            <begin position="715"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1373" mass="151568" checksum="F7F9273072159C19" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQT0</accession>
    <name>A0A452HQT0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mannosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Alpha-1,2-mannosyltransferase that catalyzes the transfer of the third mannose, via an alpha-1,2 bond, from a dolichol-phosphate-mannose (Dol-P-Man) to an alpha-D-Man-(1-&gt;6)-2-PEtn-alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol intermediate to generate an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)-2-PEtn-alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol (also termed H6) and participates in the nineth step of the glycosylphosphatidylinositol-anchor biosynthesis. May also add the third mannose to an alpha-D-Man-(1-&gt;6)-alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol (also termed H3) intermediate generating an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)-alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol (also termed H4).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452HQT0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017406"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019855.1">
        <property type="protein sequence ID" value="ENSGAGP00000017406.1"/>
        <property type="gene ID" value="ENSGAGG00000012947.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF4">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="369"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="391"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="419"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="573" mass="66271" checksum="F9BEA0334A84C333" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQY8</accession>
    <name>A0A452HQY8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin thioesterase OTU</fullName>
            <ecNumber evidence="10">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Cleaves both polyubiquitin and di-ubiquitin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452HQY8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017466"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019917.1">
        <property type="protein sequence ID" value="ENSGAGP00000017466.1"/>
        <property type="gene ID" value="ENSGAGG00000012974.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22745">
        <property type="entry name" value="OTU_OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17059">
        <property type="entry name" value="Ubl_OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000096">
        <property type="entry name" value="Ubiquitin thioesterase OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000006">
        <property type="entry name" value="Ubiquitin thioesterase OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048857">
        <property type="entry name" value="OTU1_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057766">
        <property type="entry name" value="Znf-C2H2_OTU1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312">
        <property type="entry name" value="HIV-INDUCED PROTEIN-7-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312:SF0">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21403">
        <property type="entry name" value="OTU1_UBXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24560">
        <property type="entry name" value="zf-C2H2_OTU1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0834">Unfolded protein response</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="OTU" evidence="11">
        <location>
            <begin position="105"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="304" mass="33772" checksum="EB14C15A7F8408C3" modified="2019-05-08" version="1">MLRLRCKARSGSHPLPGLTAHSRLRELQAALAALTGVPAPAQRLLLGFPPRSLDLSDGERRLGELGIHSGDTLIVEEDTTKPKTESPVVAKRSVPTLVREALPVLARRVVPADNSCLFTSIFYVVEGGIYDPACAPEMRSLIAQIVASDPESYCEAVLGKTNEEYCEWIRREDTWGGAIEISILSKFYQCEICVVDTQTVRIDRFGEDAGYARRVLLIYDGIHYDPLERKIPHSDIPPLTIFSTNDDIVLAQALELADEARRKRQFTDVNRFTLRCMVCQKGLTGQVEAREHAKETGHANFGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSN2</accession>
    <name>A0A452HSN2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dedicator of cytokinesis C/D N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HSN2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018055"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020590.1">
        <property type="protein sequence ID" value="ENSGAGP00000018055.1"/>
        <property type="gene ID" value="ENSGAGG00000013385.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021816">
        <property type="entry name" value="DOCK_C/D_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11878">
        <property type="entry name" value="DOCK_C-D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dedicator of cytokinesis C/D N-terminal" evidence="1">
        <location>
            <begin position="59"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="145" mass="16931" checksum="BC68BDD240622FE4" modified="2019-05-08" version="1">MGCAASIVLLQAFRSVVQRNCPHICRRRQEELSHEILPLDSDDEMNRPRTLTIMQEKPKLLEPLDYETIITEIERNIGDDPLKDLITFPNDDFSTDTIPWEIRTLYSSVPEDAEQNVENLFVKEVNIQHRYSLILNKSNHPFCYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWD8</accession>
    <name>A0A452HWD8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HWD8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019402"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022099.1">
        <property type="protein sequence ID" value="ENSGAGP00000019402.1"/>
        <property type="gene ID" value="ENSGAGG00000014318.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF30">
        <property type="entry name" value="GAP JUNCTION BETA-4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="11">
        <location>
            <begin position="27"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="12">
        <location>
            <begin position="126"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="259" mass="29979" checksum="46C42D97C69F3DD1" modified="2019-05-08" version="1">MNWSWLQELLSRVNKYSTAFGRIWLSVVFVFRFLVYMVAADQPGCSNVCYDHFFPVSHIRLWALQLILVTCPTLLVVMHVAYREAKQRKRQEAEGKNCRRLYPDLGKKRGGLWWTYLLSLVFKVAVDMTFLCIFHRLYANFNLPCVVQCTEAPCPNTVDCFIARPTEKKLFTYFMVATAILCILLGLCEMTYLVAKQCWELATWLHHPQGRLHSGPSLYIAERVLLVKGKEAPTSSPCIHYLLHRLQPPVQTSHQLHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HY16</accession>
    <name>A0A452HY16_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase/retrotransposon-derived protein RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HY16"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020022"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022810.1">
        <property type="protein sequence ID" value="ENSGAGP00000020022.1"/>
        <property type="gene ID" value="ENSGAGG00000014757.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13241" checksum="F7BA98A179DB8D61" modified="2019-05-08" version="1">MDLSPLSGDWLKPLHELTKEESEEPLHPNSEQIRAFKALKESLIRAPALGLPNYSKPFDLYVHEVKGVASGVLTQSFGNAPRPGHPGCLRNLAAAALLVEKSQEIVLGHPLTVQLPMQGKAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYU9</accession>
    <name>A0A452HYU9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYU9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020416"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023263.1">
        <property type="protein sequence ID" value="ENSGAGP00000020416.1"/>
        <property type="gene ID" value="ENSGAGG00000015047.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000044">
        <property type="entry name" value="Kelch-like family member 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF255">
        <property type="entry name" value="KELCH-LIKE PROTEIN 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="8">
        <location>
            <begin position="35"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="596" mass="66055" checksum="32BB3A75A0EA411F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZD0</accession>
    <name>A0A452HZD0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZD0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020598"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023462.1">
        <property type="protein sequence ID" value="ENSGAGP00000020598.1"/>
        <property type="gene ID" value="ENSGAGG00000015168.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF206">
        <property type="entry name" value="NUCLEASE HARBI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="9">
        <location>
            <begin position="204"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="421" mass="47962" checksum="BA6F167934F5D789" modified="2019-05-08" version="1">MDPEQLTTLLMNVASTSRLAVELFLQLQNESESESDSESEESDDDIESPHCEDSTLLVAVTDVLSSVDRRVWARETSTQWWDQIVLQSWDDEQWLQNFRMRKATFMALCAELAPTLRRRDTRLRAALPVEKRVAIAIWKLATPDCFRSVANQFGVGKSTVGMVLMQVCTAINRTLLRRTVTLGNVQDIVDGFAEMGFPNCGGAIDGTHIPILSPPHLASEYVNRKGYFSVVLQALVDHRGRFTDIYSGWPGKVHDARIFRNSALFRRLRAGTFFPDRKITVGDVEMPTVILGDPAYPLMPWLMKPYTGKLDRSKDRFNYRLSRCRMTVECAFGRLKARWRCLYGKLDLGESSISAVISACCTLHNICEGKGERFSEEWTSEVRRLEDEFAQPESRANREAQERASRIRDALREQFDAESQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0J2</accession>
    <name>A0A452I0J2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000020957"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023871.1">
        <property type="protein sequence ID" value="ENSGAGP00000020957.1"/>
        <property type="gene ID" value="ENSGAGG00000015401.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032770">
        <property type="entry name" value="DUF4537"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46785">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46785:SF1">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15057">
        <property type="entry name" value="DUF4537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="3">
        <location>
            <begin position="514"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1193"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="916"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1206"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1222"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1243"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1265"/>
            <end position="1274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1306" mass="148833" checksum="F2E7C0A668C8B86B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0K9</accession>
    <name>A0A452I0K9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the complex I LYR family. SDHAF1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I0K9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020978"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023895.1">
        <property type="protein sequence ID" value="ENSGAGP00000020978.1"/>
        <property type="gene ID" value="ENSGAGG00000015415.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20268">
        <property type="entry name" value="Complex1_LYR_SDHAF1_LYRM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045295">
        <property type="entry name" value="Complex1_LYR_SDHAF1_LYRM8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052687">
        <property type="entry name" value="SDHAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47046">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47046:SF1">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="6">
        <location>
            <begin position="9"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="119" mass="14024" checksum="CAB01699B62D85F8" modified="2019-05-08" version="1">MARHSKLQKQVLSLYRQFLRAGKEKPGFLPRIQAEFRKNASIPRMDVMHIEYLLRRGQRQLKQLRDVNTKQMGGFRSTLWLDSAAAPKNHRTVRLEGTWRDRHEKPPALRQGQVNPAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1V3</accession>
    <name>A0A452I1V3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell cycle exit and neuronal differentiation 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I1V3"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024444.1">
        <property type="protein sequence ID" value="ENSGAGP00000021462.1"/>
        <property type="gene ID" value="ENSGAGG00000015760.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021686">
        <property type="term" value="P:cerebellar granular layer maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021702">
        <property type="term" value="P:cerebellar Purkinje cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021933">
        <property type="term" value="P:radial glia guided migration of cerebellar granule cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020162">
        <property type="entry name" value="Cend1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36683">
        <property type="entry name" value="CELL CYCLE EXIT AND NEURONAL DIFFERENTIATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36683:SF1">
        <property type="entry name" value="CELL CYCLE EXIT AND NEURONAL DIFFERENTIATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15677">
        <property type="entry name" value="CEND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="13329" checksum="3785089FBE13F885" modified="2019-05-08" version="1">MESKGSTRSGSKPDAKAASSGKPEKPIPGPAMNADKKETPSKEQPTPVATTKKAASEATALNNHSNLKPNPAAPEVQEATGQSPDSEHKGNGTEESSGSVFDNMKPLAIVGGVVVAAVAIILGVAFLARKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2A8</accession>
    <name>A0A452I2A8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I2A8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021664"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024676.1">
        <property type="protein sequence ID" value="ENSGAGP00000021664.1"/>
        <property type="gene ID" value="ENSGAGG00000015900.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000040">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000383">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000056">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF416">
        <property type="entry name" value="FI01424P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="553"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="581"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="611"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="635" mass="67824" checksum="BD89855A89176FDD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4Z0</accession>
    <name>A0A452I4Z0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000022641"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025794.1">
        <property type="protein sequence ID" value="ENSGAGP00000022641.1"/>
        <property type="gene ID" value="ENSGAGG00000016605.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000052">
        <property type="entry name" value="Ankyrin repeat domain-containing protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053210">
        <property type="entry name" value="ANKRD12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24149">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24149:SF16">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="218"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="251"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="284"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="51"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="181"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="341"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="443"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="619"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="736"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="820"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="872"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1125"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1212"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1390"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1779"/>
            <end position="1804"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1654"/>
            <end position="1681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="207"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="487"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="501"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="514"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="581"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="629"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="639"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="669"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="688"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="741"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="824"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="872"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="907"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1005"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1089"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1147"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1779"/>
            <end position="1793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2099" mass="239428" checksum="643C14E9ECE4AFD0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6J4</accession>
    <name>A0A452I6J4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatase and actin regulator 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphatase and actin regulator family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I6J4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023245"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026482.1">
        <property type="protein sequence ID" value="ENSGAGP00000023245.1"/>
        <property type="gene ID" value="ENSGAGG00000017045.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043149">
        <property type="term" value="P:stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004018">
        <property type="entry name" value="RPEL_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751:SF6">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR PHACTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00707">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="105" mass="12335" checksum="B9716E39C57A8B92" modified="2019-05-08" version="1">TSNLQKKTSVAENGKRREEDFSVPFLPLYLQLSQRPTVEELREKKILIRFSDYVEVADAQDYDRRADKPWTRLSAADKAAIRKELNEFKSTEMEVHELSKHLTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I802</accession>
    <name>A0A452I802_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein phosphatase 1 regulatory subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">RNA polymerase II subunit 5-mediating protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival signaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity in mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homodimer. Component of the PAQosome complex which is responsible for the biogenesis of several protein complexes and which consists of R2TP complex members RUVBL1, RUVBL2, RPAP3 and PIH1D1, URI complex members PFDN2, PFDN6, PDRG1, UXT and URI1 as well as ASDURF, POLR2E and DNAAF10/WDR92. Interacts with POLR2E/RPB5, RUVBL2 and RUVBL1. Interacts with PFDN2, PFDN4 and STAP1; the interactions are phosphorylation-dependent and occur in a growth-dependent manner in the mitochondrion. Interacts with UXT. Interacts with PPP1CC; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner. Interacts (via the middle C-terminal region) with GTF2F1 and GTF2F2. Interacts with DMAP1. Interacts with TSC1 and TSC2. Interacts with PRPF8 and EFTUD2 in a ZNHIT2-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the RNA polymerase II subunit 5-mediating protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I802"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023768"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027071.1">
        <property type="protein sequence ID" value="ENSGAGP00000023768.1"/>
        <property type="gene ID" value="ENSGAGG00000017395.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23159">
        <property type="entry name" value="Prefoldin_URI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000008">
        <property type="entry name" value="unconventional prefoldin RPB5 interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052255">
        <property type="entry name" value="RNA_pol_II_subunit5-mediator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT 5-MEDIATING PROTEIN NNX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111:SF0">
        <property type="entry name" value="UNCONVENTIONAL PREFOLDIN RPB5 INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="148"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="473"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="501" mass="56805" checksum="A27497E529E7D57E" modified="2019-05-08" version="1">MQGPGTASPLSAAQVGRLRQEQGKVVTSCQEKIQHWRKVENDYEALQERLNTLPDKLSYDIMVPFGPLAFMPGKLVHTNEVTVLLGDNWFSKCSAKQAIGLVEHRKKHVRKALEDLQKVMKNFESRIEFTEDLQKMSDAAGDIVDIREEAENDGTETKGKRRTAHKPHSKPKVSDVFEVHFQENGMDTKFTGYSQSEEELWARLEELERREEMLGEFNRTPDTTEANGEDTTSSEEEKEDKTDLNVVHRVEDSTTQDNVQKEVTNSELFSGQVNGRSHYHSDDEDDLGVDHFVPTIFFSHTVEPKRVRINTGKNTTLKFSEKKEEAKRKRKNNNGNGHLVPELPNIKTPADIYRVFVDVVNGEYVPRKSILKSRSRENSVCSDTSESSAAEFDDRRGVLRSISYDEATYSDNSEGILEEEEEEGQEHLPKKLSTLSGTFEAFSGTVIEKEPLSPLIPHPSIAHPVLPTIPERKSEEVLSEASEETTKRISKFKAARMQQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I818</accession>
    <name>A0A452I818_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein 29</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Axonemal protein which is implicated in axonemal and/or peri-axonemal structure assembly and regulates flagellum assembly and beating and therefore sperm motility.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I818"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023781"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027087.1">
        <property type="protein sequence ID" value="ENSGAGP00000023781.1"/>
        <property type="gene ID" value="ENSGAGG00000017404.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051476">
        <property type="entry name" value="Bac_ResReg_Asp_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46630">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46630:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="354"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="470" mass="53574" checksum="19AB6319A13D7A2F" modified="2019-05-08" version="1">MSTLPPVLIQCPKRYTSLVEQMVCPSIKLARIHLIKDKDAIDHYLSMNIKGLSKQEAAAYRNSYKKNICIDMLRQGYHKSFSELFTLIQKWNALREAAGPGSAIWQQKSLEEQPDKLDQLHHFLTRAEAAQRAEYYEEVYNNQLKLAYCFNNTEDKWLRNYFYEQCLSTAQLIKIDGGKKESEAHANMGLVNEEQGLFMKAAEHYETFHQLTLGRMWNDETGHTYNSLACEHLWRIYTLLADKMLENKEHQQAIKTLIKAFEMAKKGGDKKMEGEAAYCLGLAYHSAGEEQTAIITLNTYKEISTELCDIVGLGRAYQAIAKVLVSQVNVTEAIEYLEKFVTVAKSAQLSRSLVDACISLGDIYNERGNYYKACEYFNQAFEAANTLTDLPLVDETKVYYGIAKAHNMMVAVNSHTDAADHVSIEYLLAWKENRSDMCTDPFTVELAKDTSDVLETNELFLQTPVQEPLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9T6</accession>
    <name>A0A452I9T6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the nuclear hormone receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I9T6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024413"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027787.1">
        <property type="protein sequence ID" value="ENSGAGP00000024413.1"/>
        <property type="gene ID" value="ENSGAGG00000017835.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050728">
        <property type="term" value="P:negative regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06968">
        <property type="entry name" value="NR_DBD_ROR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06939">
        <property type="entry name" value="NR_LBD_ROR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000005">
        <property type="entry name" value="Nuclear orphan receptor ROR-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000003">
        <property type="entry name" value="Nuclear orphan receptor ROR-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044101">
        <property type="entry name" value="NR_DBD_ROR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003079">
        <property type="entry name" value="ROR_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45805">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR HR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45805:SF3">
        <property type="entry name" value="NUCLEAR RECEPTOR ROR-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01293">
        <property type="entry name" value="RORNUCRECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="15"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="217"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="432" mass="49303" checksum="BD8DA97F2365B461" modified="2019-05-08" version="1">MMCFVIAAMKAQIEIIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQSNATYSCPRQKNCLIDRTSRNRCQHCRLQKCLAVGMSRDAVKFGRMSKKQRDSLYAEVQKHRMQQQQRDHQQQPGEAEPLTPTYSITTNGLTELHDDLSNYIDGHTPEGSKADSAVSSFYLDIQPSPDQSGLDINGIKPEPICDYTPASGFFPYCSFTNGETSPTVSMAELEHLAQNISKSHMETCQYLREELQQITWQTFLQEEIENYQNKQREVMWQLCAVKITEAIQYVVEFAKRIDGFMELCQNDQIVLLKAGSLEVVFIRMCRAFDSQNNTVYFDGKYASPDVFKSLGCEDFISFVFEFGKSLCSMHLTEDEIALFSAFVLMSADRSWLQEKVKIEKLQQKIQLALQHVLQKNHREDGILTKVSTKNCALSSEHESILHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IA65</accession>
    <name>A0A452IA65_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Actin related protein 2/3 complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARPC4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IA65"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024584"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027980.1">
        <property type="protein sequence ID" value="ENSGAGP00000024584.1"/>
        <property type="gene ID" value="ENSGAGG00000017952.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008384">
        <property type="entry name" value="ARPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22629:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22629">
        <property type="entry name" value="ARP2/3 COMPLEX 20 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05856">
        <property type="entry name" value="ARPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="21636" checksum="A5BC10B38A139992" modified="2019-05-08" version="1">MERSAHPGREEPPRLHPPLPLPGSRSDRSEAAAMTATLRPYLNAVRATLQAALCLENFSSQVVERHNKPEVEVRSSKELLLQPVIISRNEKEKVLIEGSINSVRVSIAVKQADEIEKILCHKFMRFMMMRAENFFILRRKPVEVRWLGWGANPVVQDRDTSTHLLWQLTLMSIFATVLSCQGGQRGFGGDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBM9</accession>
    <name>A0A452IBM9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IBM9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025142"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028628.1">
        <property type="protein sequence ID" value="ENSGAGP00000025142.1"/>
        <property type="gene ID" value="ENSGAGG00000018396.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004920">
        <property type="term" value="F:interleukin-10 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015373">
        <property type="entry name" value="Interferon/interleukin_rcp_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050650">
        <property type="entry name" value="Type-II_Cytokine-TF_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859">
        <property type="entry name" value="INTERFERON/INTERLEUKIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859:SF50">
        <property type="entry name" value="INTERLEUKIN-10 RECEPTOR SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09294">
        <property type="entry name" value="Interfer-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01108">
        <property type="entry name" value="Tissue_fac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018987701" description="Fibronectin type-III domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="21"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="112"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="359" mass="40891" checksum="CBE61967817933EA" modified="2019-05-08" version="1">MARRFLALVAGCLLCSAFGIVPEPKNVTINSVNLKSTLQWDPLTIHKENITYTVESISSLYNRNMCVNIVITECDFSSLPVYGEYTLRVRAESENEKSDWVNIIFKPLDDTIIGPPDVKVKSESGSLHVDFTGPVAVQESHVWPLNKYYGSWVYRVLYWKKGNSEEVITTDTKYNSEILSGLDPWTIYCLQVQAVIPEWNKTGKLSEEFCDRTTDNGVTPVWIILTVLLVSMLVVLVSVPACFFIFSYIYRQARYVFCPTYSFPQHLKEFLSKPSYSSQFLSPCSPEEDHSFDKLTVISEESENCSDETSNRKEQLQDSQEESSIAEKVSAFEKGPSSVLYLIQAENKYPNRQLNTTLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBX6</accession>
    <name>A0A452IBX6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box only protein 39</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Directly interacts with SKP1 and CUL1.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IBX6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025018"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028479.1">
        <property type="protein sequence ID" value="ENSGAGP00000025018.1"/>
        <property type="gene ID" value="ENSGAGG00000018289.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22108">
        <property type="entry name" value="F-box_FBXO39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000027">
        <property type="entry name" value="F-box only protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000237">
        <property type="entry name" value="F-box only protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045048">
        <property type="entry name" value="FBXO31/39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706">
        <property type="entry name" value="F-BOX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706:SF160">
        <property type="entry name" value="F-BOX ONLY PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="9"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00062469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="439" mass="51845" checksum="089F1242E69B241D" modified="2019-05-08" version="1">MEDDSEQEQSCWAYLPDVCLSNMFWWLDDRDRSRAALVCKKWNQAMYSGSLWRTRTITFSGRPSRSNTSEFESALWYVKRFGKYLEHLEIKFLNPYNAVLTRKFQVTMRGLLSRLGKCNSRLVSLTIQHLELDRLVWKNGIRTQFIKNISTFLKRMSKHLDYLNLKGARVMLEEGCEILSSLSYLKNKSFASEINIEDFFSHHLSIYSSPLFHQTMSTFRNLVILTLNYNCISDELLDILCENNAHSLWTINIKCHTHDPHGQVVWGMSWANLAKRAPKLKVNFFFERVMKHNSLARILLAEIPLRSISLRSCYFSDPDWSMRPTLTTLLPAYKHSLQKLTLEFNNDHELLDEELLQLVLLCERLFFLKVWAFLSVAFVERLLQYRAEGKCVLCTMKVRIYTTRHETSEEDRLLRDIYKKFRNLIDSELNYFVIAYPMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ID82</accession>
    <name>A0A452ID82_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ID82"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025477"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028997.1">
        <property type="protein sequence ID" value="ENSGAGP00000025477.1"/>
        <property type="gene ID" value="ENSGAGG00000018622.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031677">
        <property type="entry name" value="TEX38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37362">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37362:SF1">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15834">
        <property type="entry name" value="THEG4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="194" mass="22006" checksum="9E1EB90E8BEE3B1F" modified="2019-05-08" version="1">SPMSTALTLCSCFLGWLPLYFAGLGLCYILIFSSMFFFHWKKVVQREQRAQEWVELMRSETFFPNAVVHWANKRAHYGIKAEVCTPESSASPKQNGKSFDRDTTTGRDGAFFPSLTGVGSYKLVFQELPWACSLSSLPPLLHHSTSYPFSAIPSRTVPFSSLPDFVTLGNVRWFHFAKPFTDLTTSREYLLVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDM2</accession>
    <name>A0A452IDM2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDM2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025960"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029528.1">
        <property type="protein sequence ID" value="ENSGAGP00000025960.1"/>
        <property type="gene ID" value="ENSGAGG00000018957.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="60"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="235" mass="25353" checksum="A045B37A3ED4618E" modified="2019-05-08" version="1">PKAGSRVPGHGADSWGQQPGPGPGPGPGAELDGAPPSLQVGSGPGPTICLGIIGSLGFLNNLLVLILFCRNKVLWSPINLLLMNISVSDMMVCIMGTPFSFAASTQGRWLIGPGGCIWYGFINTLFGTVSLISLAVLSYERYCTMMGSTEADATNYRKIWMGIFLSWMYSLFWTLPPLFGWSSYGPEGPGTTCSVNWHSRDWLAPWNSWSTPTAAAVQIMKGQIIYPTYVYGPEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFT8</accession>
    <name>A0A452IFT8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Myelin transcription factor 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYT1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IFT8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026344"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029953.1">
        <property type="protein sequence ID" value="ENSGAGP00000026344.1"/>
        <property type="gene ID" value="ENSGAGG00000019082.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.320.30:FF:000001">
        <property type="entry name" value="Myelin transcription factor 1-like, a"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.30">
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013681">
        <property type="entry name" value="Myelin_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002515">
        <property type="entry name" value="Znf_C2H2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036060">
        <property type="entry name" value="Znf_C2H2C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816:SF11">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08474">
        <property type="entry name" value="MYT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01530">
        <property type="entry name" value="zf-C2HC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103637">
        <property type="entry name" value="CCHHC domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51802">
        <property type="entry name" value="ZF_CCHHC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Myelin transcription factor 1" evidence="14">
        <location>
            <begin position="618"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="342"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="386"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="447"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="660"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1074"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="88"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="358"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="397"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="447"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="492"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="663"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="681"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1202" mass="135073" checksum="053CFB9050045EAE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGA4</accession>
    <name>A0A452IGA4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U5 small nuclear ribonucleoprotein 40 kDa protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">WD repeat-containing protein 57</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for pre-mRNA splicing as component of the activated spliceosome. Component of the U5 small nuclear ribonucleoprotein (snRNP) complex and the U4/U6-U5 tri-snRNP complex, building blocks of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the pre-catalytic and catalytic spliceosome complexes. Component of the postcatalytic spliceosome P complex. Part of the U5 snRNP complex. Interacts with PRPF8. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, WDR57, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Component of the minor spliceosome, which splices U12-type introns.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IGA4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026795"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030457.1">
        <property type="protein sequence ID" value="ENSGAGP00000026795.1"/>
        <property type="gene ID" value="ENSGAGG00000019518.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000375">
        <property type="term" value="P:RNA splicing, via transesterification reactions"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000229">
        <property type="entry name" value="Small nuclear ribonucleoprotein U5 subunit 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052234">
        <property type="entry name" value="U5_snRNP_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006:SF1">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="63"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="106"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="148"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="190"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="232"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="293"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="324"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="358" mass="39175" checksum="CFCB6E322D8960E5" modified="2019-05-08" version="1">MIEQQKRKGPGPELALVAAVAAKRPRHELLLGPGPGGGPPPPGALLQAGPPRCSSLQAPIMLLSGHEGEVYCCKFHPNGATLASAGFDRLILLWHVYGDCDNFATLKGHSGAVMELHYNTDGSMLFSASTDKTVAVWDSETGERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWDIRKKAAVQTFQNTYQVLAVTFNDTSDQIISGGIDNDIKVWDLRQNKLTYTMRGHADSVTGLSLSSEGSYLLSNAMDNTVRIWDVRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGSKIAGGSADRFVYVWDTTSRRILYKLPGHAGSVNEVAFHPEEPIILSASSDKRLYMGEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHC8</accession>
    <name>A0A452IHC8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Microcephalin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Implicated in chromosome condensation and DNA damage induced cellular responses. May play a role in neurogenesis and regulation of the size of the cerebral cortex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with CDC27 and maybe other components of the APC/C complex. Interacts with histone variant H2AX under DNA damage conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IHC8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027359"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031081.1">
        <property type="protein sequence ID" value="ENSGAGP00000027359.1"/>
        <property type="gene ID" value="ENSGAGG00000019895.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021987">
        <property type="term" value="P:cerebral cortex development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17716">
        <property type="entry name" value="BRCT_microcephalin_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022047">
        <property type="entry name" value="Microcephalin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029504">
        <property type="entry name" value="Microcephalin_mammal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14625">
        <property type="entry name" value="MICROCEPHALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14625:SF3">
        <property type="entry name" value="MICROCEPHALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12258">
        <property type="entry name" value="Microcephalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BRCT" evidence="10">
        <location>
            <begin position="33"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="541"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="542"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="568"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="584" mass="65825" checksum="977CCED39C6D8383" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHG1</accession>
    <name>A0A452IHG1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452IHG1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027345"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031060.1">
        <property type="protein sequence ID" value="ENSGAGP00000027345.1"/>
        <property type="gene ID" value="ENSGAGG00000019881.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="57"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="283" mass="32831" checksum="A0BCC76AF1AE5CB0" modified="2019-05-08" version="1">MTTARKKFIERLDQAMATAEKMNPEDLLCSYKGVLYPVTHCSAETFKALESFEARSDDVLLVGYPKTGTNWIIHILRDLVTTSDEEIVEYTYLEFGDPGKFQRIKNLSSRRVFATHIFPHMLPTSIFKNNVKILMVIRNPKDIAVSYFHFSNSMPSLPSFQTWDEFFNAFLNGKVSWGSYFDYIIEWNKHFDDENIMFITYEELKENPNLGVKKIAEFFGFSVTDEESQAVADRSSFQTMKENSHKTHGAFGNVLFRKGSVSDWKNLFTEAQNQEMDKKFEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHI6</accession>
    <name>A0A452IHI6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IHI6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027430"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031160.1">
        <property type="protein sequence ID" value="ENSGAGP00000027430.1"/>
        <property type="gene ID" value="ENSGAGG00000019940.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09938">
        <property type="entry name" value="SH2_N-SH2_Zap70_Syk_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.930.10:FF:000001">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000031">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.930.10">
        <property type="entry name" value="Syk Kinase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043539">
        <property type="entry name" value="Grb2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023420">
        <property type="entry name" value="Kinase_SYK/ZAP-70_inter-SH2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035838">
        <property type="entry name" value="SYK/ZAP-70_N_SH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46037">
        <property type="entry name" value="PROTEIN ENHANCER OF SEVENLESS 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="2">
        <location>
            <begin position="10"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="177" mass="20049" checksum="6D0417845E87B402" modified="2019-05-08" version="1">MPDAASHLPFFYGSISRSEAEEYLKLAGMSDGLFLLRQCLRSLGGYVLSMVHNLQFYHYPIERQLNGTYAIAGGKAHCGPEELCKFYSKDADGLCCTLRKPCNRPSGVALQPGVFDSMRDNMVREYIRQTWKLEGDALEQAIISQAPQVEKLIATTAHEKMPWYHSNISREEAERRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHQ3</accession>
    <name>A0A452IHQ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IHQ3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027401"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031128.1">
        <property type="protein sequence ID" value="ENSGAGP00000027401.1"/>
        <property type="gene ID" value="ENSGAGG00000019922.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="10222" checksum="D46FCE80307D3D8E" modified="2019-05-08" version="1">MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEVMGPKKKHLDCEYRGVCGRGSPGSRGVCEYRGVCGRGSPGSRGVCECRGEGRGICEYRGGGVWGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJ84</accession>
    <name>A0A452IJ84_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000027895"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031684.1">
        <property type="protein sequence ID" value="ENSGAGP00000027895.1"/>
        <property type="gene ID" value="ENSGAGG00000019965.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019898">
        <property type="term" value="C:extrinsic component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13240">
        <property type="entry name" value="PH1_Kalirin_Trio_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11852">
        <property type="entry name" value="SH3_Kalirin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide exchange factor DBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000040">
        <property type="entry name" value="kalirin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000034">
        <property type="entry name" value="kalirin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000003">
        <property type="entry name" value="kalirin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000024">
        <property type="entry name" value="Kalirin RhoGEF kinase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000023">
        <property type="entry name" value="Kalirin RhoGEF kinase b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000032">
        <property type="entry name" value="Kalirin RhoGEF kinase b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000040">
        <property type="entry name" value="Kalirin RhoGEF kinase b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000015">
        <property type="entry name" value="triple functional domain protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047054">
        <property type="entry name" value="Kalirin_TRIO_PH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028570">
        <property type="entry name" value="Kalirin_TRIO_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058918">
        <property type="entry name" value="KALRN/TRIO-like_spectrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051336">
        <property type="entry name" value="RhoGEF_Guanine_NuclExch_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826">
        <property type="entry name" value="RHO GUANINE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826:SF106">
        <property type="entry name" value="TRIO, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23323">
        <property type="entry name" value="Spectrin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 12" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="18">
        <location>
            <begin position="29"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="17">
        <location>
            <begin position="1262"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="16">
        <location>
            <begin position="1449"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="1627"/>
            <end position="1692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1575"/>
            <end position="1623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1841"/>
            <end position="2132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="900"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1608"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1863"/>
            <end position="1874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1880"/>
            <end position="1898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="1907"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1982"/>
            <end position="2015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="2016"/>
            <end position="2028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="2032"/>
            <end position="2052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="2062"/>
            <end position="2080"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2132" mass="243222" checksum="B88AC4F1F3362A55" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJR6</accession>
    <name>A0A452IJR6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Receptor expression-enhancing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJR6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028068"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031884.1">
        <property type="protein sequence ID" value="ENSGAGP00000028068.1"/>
        <property type="gene ID" value="ENSGAGG00000020391.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071782">
        <property type="term" value="C:endoplasmic reticulum tubular network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF36">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="210" mass="24496" checksum="BA0A4C12EFE7E5EF" modified="2019-05-08" version="1">MVSWIICRVVVLVFGMLYPAYASYKAVKTKNIREYVRWMMYWIVFALFMATETLTDTFVSWFPFYYEIKMAFVVWLLSPYTKGASLLYRKFVHPTLSHREKEIDLYIAQAKERGYETMVNFGKKSLNIAATAAVQAAAKSQGALAGRLRSFSMQDLRAIPDDAPVHYEDPLYLEEQEMRRRPIGERRERGGERTKFWGSRVCKISNFPGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJW4</accession>
    <name>A0A452IJW4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(11Z)-octadecenoyl-CoA + cholesterol = cholesteryl (11Z)-octadecenoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:64324"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:75121"/>
            <dbReference type="ChEBI" id="CHEBI:88768"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:64325"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:42816"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:82751"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:42817"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(7Z)-octadecenoyl-CoA + cholesterol = cholesteryl (7Z)-octadecenoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:64328"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:152049"/>
            <dbReference type="ChEBI" id="CHEBI:152050"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:64329"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(9Z)-hexadecenoyl-CoA + cholesterol = cholesteryl (9Z)-hexadecenoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:64320"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61540"/>
            <dbReference type="ChEBI" id="CHEBI:84323"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:64321"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(9Z,12Z)-octadecadienoyl-CoA + cholesterol = cholesteryl (9Z,12Z)-octadecadienoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:42796"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:41509"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:42797"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>a sterol + a long-chain fatty acyl-CoA = a long-chain 3-hydroxysterol ester + CoA</text>
            <dbReference type="Rhea" id="RHEA:59816"/>
            <dbReference type="ChEBI" id="CHEBI:15889"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:232093"/>
            <dbReference type="EC" id="2.3.1.26"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:59817"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>cholesterol + (9Z)-octadecenoyl-CoA = cholesteryl (9Z-octadecenoate) + CoA</text>
            <dbReference type="Rhea" id="RHEA:41436"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:46898"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:41437"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>an acyl-CoA + cholesterol = a cholesterol ester + CoA</text>
            <dbReference type="Rhea" id="RHEA:17729"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17002"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:17730"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>cholesterol + hexadecanoyl-CoA = cholesteryl hexadecanoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:42792"/>
            <dbReference type="ChEBI" id="CHEBI:3663"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:42793"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>octadecanoyl-CoA + cholesterol = cholesteryl octadecanoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:42812"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:82750"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:42813"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 25">Endoplasmic reticulum membrane</location>
            <topology evidence="2 25">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 25">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJW4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028168"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032006.1">
        <property type="protein sequence ID" value="ENSGAGP00000028166.1"/>
        <property type="gene ID" value="ENSGAGG00000020468.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000032008.1">
        <property type="protein sequence ID" value="ENSGAGP00000028168.1"/>
        <property type="gene ID" value="ENSGAGG00000020468.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034736">
        <property type="term" value="F:cholesterol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033344">
        <property type="term" value="P:cholesterol efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030687">
        <property type="entry name" value="Sterol_acyltranf_meta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF6">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500230">
        <property type="entry name" value="Sterol_acyltranf_ACAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 25" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 25" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 25 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 28" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="177"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="364"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="504"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="active site" evidence="26">
        <location>
            <position position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00037015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRNR" id="PIRNR000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="553" mass="64584" checksum="FB8CE18AEF3339F5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IK59</accession>
    <name>A0A452IK59_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">iodotyrosine deiodinase</fullName>
            <ecNumber evidence="2">1.21.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>3-iodo-L-tyrosine + iodide + NADP(+) = 3,5-diiodo-L-tyrosine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:57506"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59898"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="8">
            <dbReference type="Rhea" id="RHEA:27459"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-tyrosine + chloride + NADP(+) = 3-chloro-L-tyrosine + NADPH</text>
            <dbReference type="Rhea" id="RHEA:70343"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:189422"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:70345"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>bromide + L-tyrosine + NADP(+) = 3-bromo-L-tyrosine + NADPH</text>
            <dbReference type="Rhea" id="RHEA:70347"/>
            <dbReference type="ChEBI" id="CHEBI:15858"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:189423"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="7">
            <dbReference type="Rhea" id="RHEA:70349"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>iodide + L-tyrosine + NADP(+) = 3-iodo-L-tyrosine + NADPH</text>
            <dbReference type="Rhea" id="RHEA:27453"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59898"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="6">
            <dbReference type="Rhea" id="RHEA:27455"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nitroreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.21.1.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452IK59"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028266"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032121.1">
        <property type="protein sequence ID" value="ENSGAGP00000028266.1"/>
        <property type="gene ID" value="ENSGAGG00000020537.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140616">
        <property type="term" value="F:iodotyrosine deiodinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006570">
        <property type="term" value="P:tyrosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02144">
        <property type="entry name" value="iodotyrosine_dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050627">
        <property type="entry name" value="Nitroreductase/BluB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23026:SF90">
        <property type="entry name" value="IODOTYROSINE DEIODINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23026">
        <property type="entry name" value="NADPH NITROREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="Nitroreductase" evidence="11">
        <location>
            <begin position="103"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="265" mass="30636" checksum="FC0BA802DFF8756E" modified="2019-05-08" version="1">MELFSSLTPVFVAIICVLIGIILKSNRKNSKKGKSETKNKSESRRWVDDDLRDSTDDLHVEEEDADGDWQGLDENIAHVSFSAEHYSEGEMIKRSKEFYELLNKRRSVRFISDEPVPREIINNVIKAAGTSPSGAHTEPWTFVVVQDPEVKHRIREIIEEEEEINYKKRMGDRWVNDLKRLRTNWIKEYLDTAPYLILIFKQVYGMLPNGQKKTHYYNEISVSIACGILLAALQVRIGLTLHPGRSQLWCIMAWSAQMCPASRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKS6</accession>
    <name>A0A452IKS6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat domain-containing protein 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Diversin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Recruits CKI-epsilon to the beta-catenin degradation complex that consists of AXN1 or AXN2 and GSK3-beta and allows efficient phosphorylation of beta-catenin, thereby inhibiting beta-catenin/Tcf signals.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with AXN1, AXN2 and CSNK1E/CKI-epsilon.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IKS6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028503"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032373.1">
        <property type="protein sequence ID" value="ENSGAGP00000028503.1"/>
        <property type="gene ID" value="ENSGAGG00000020653.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000293">
        <property type="entry name" value="Ankyrin repeat domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000370">
        <property type="entry name" value="Ankyrin repeat domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000357">
        <property type="entry name" value="ankyrin repeat domain-containing protein 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF185">
        <property type="entry name" value="ANKYRIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="41"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="74"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="107"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="140"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="173"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="206"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="277"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="602"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="675"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="760"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="280"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="356"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="686"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="699"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00081295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="792" mass="87817" checksum="3D4CE63096328AC0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMC7</accession>
    <name>A0A452IMC7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the kindlin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IMC7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029070"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033023.1">
        <property type="protein sequence ID" value="ENSGAGP00000029070.1"/>
        <property type="gene ID" value="ENSGAGG00000021034.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030055">
        <property type="term" value="C:cell-substrate junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033622">
        <property type="term" value="P:integrin activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007159">
        <property type="term" value="P:leukocyte cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033632">
        <property type="term" value="P:regulation of cell-cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13205">
        <property type="entry name" value="FERM_C_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17182">
        <property type="entry name" value="FERM_F0_KIND3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01237">
        <property type="entry name" value="PH_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000037">
        <property type="entry name" value="Fermitin family homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000057">
        <property type="entry name" value="Fermitin family homolog 2 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000035">
        <property type="entry name" value="Fermitin family homolog 2 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037843">
        <property type="entry name" value="Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040790">
        <property type="entry name" value="Kindlin_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037837">
        <property type="entry name" value="PH_Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160">
        <property type="entry name" value="FERMITIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160:SF1">
        <property type="entry name" value="FERMITIN FAMILY HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18124">
        <property type="entry name" value="Kindlin_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="359"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="659" mass="75228" checksum="D94D937EFB9DBD15" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IP08</accession>
    <name>A0A452IP08_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hexosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus</location>
            <location evidence="1 9">Golgi stack membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the chondroitin N-acetylgalactosaminyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452IP08"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029584"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033594.1">
        <property type="protein sequence ID" value="ENSGAGP00000029584.1"/>
        <property type="gene ID" value="ENSGAGG00000021368.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047238">
        <property type="term" value="F:glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008428">
        <property type="entry name" value="Chond_GalNAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051227">
        <property type="entry name" value="CS_glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369:SF40">
        <property type="entry name" value="CHONDROITIN SULFATE SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369">
        <property type="entry name" value="CHONDROITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05679">
        <property type="entry name" value="CHGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="361" mass="42329" checksum="3714ED4C77C9F958" modified="2019-05-08" version="1">MPAYQYRLHNYILSRKISELRYRTIQLHRESALMSKLSNSEINKEDQQLGMMPSFNRFQPRERNEVIEWEFLTGKLLYSVAENQPPRQSINSILRAALDDTVMQVMEMINENSKSRGRLIDFKEIQYGYRRVDPMHGVEYILDLLLLYKRHKGRKLTVPVRRHAYLQQLFSKPFFKEAEELDVRSLKIHILVPLTGRYDIFLRFMENFEKTCVIPRQNVKLAVILFSSDSGQDSSKHRELIKEYHNKYPKADMTLIPMTGDFSRGLGLEMASSQFDNDTLLLFCDVDLIFTSDFLQRCRDNTIQGQQVYYPIIFSQYDPKVIYGGNPPGDSNFQMSENEYSPVDVCHLAIALEELLICLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IP98</accession>
    <name>A0A452IP98_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Huntingtin-interacting protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Huntingtin-interacting protein I</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Regulates presynaptic nerve terminal activity. Enhances androgen receptor (AR)-mediated transcription. May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. May play a functional role in the cell filament networks. May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer. Binds actin. Binds HTT (via N-terminus). This interaction is restricted to the brain. Binds to IFT57. In normal conditions, it poorly interacts with IFT57, HIP1 being strongly associated with HTT. However, in mutant HTT proteins with a long poly-Gln region, interaction between HTT and HIP1 is inhibited, promoting the interaction between HIP1 and IFT57. Interacts with CLTB (via N-terminus). Interacts (via coiled coil domain) with AR. Interacts with AP2A1, AP2A2, CLTC and HIP1R. Interacts with GRIA1, GRIN2A and GRIN2B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SLA2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IP98"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029927"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033986.1">
        <property type="protein sequence ID" value="ENSGAGP00000029927.1"/>
        <property type="gene ID" value="ENSGAGG00000021602.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043325">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048260">
        <property type="term" value="P:positive regulation of receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010646">
        <property type="term" value="P:regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17013">
        <property type="entry name" value="ANTH_N_HIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1410.10:FF:000002">
        <property type="entry name" value="Huntingtin interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1700:FF:000002">
        <property type="entry name" value="Huntingtin interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000022">
        <property type="entry name" value="huntingtin-interacting protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032422">
        <property type="entry name" value="HIP1_clath-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035964">
        <property type="entry name" value="I/LWEQ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002558">
        <property type="entry name" value="ILWEQ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030224">
        <property type="entry name" value="Sla2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407:SF14">
        <property type="entry name" value="HUNTINGTIN-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16515">
        <property type="entry name" value="HIP1_clath_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01608">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00307">
        <property type="entry name" value="ILWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109885">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50945">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="10 21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ENTH" evidence="22">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="I/LWEQ" evidence="23">
        <location>
            <begin position="756"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="334"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="548"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="963"/>
            <end position="992"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00062629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00073538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00080332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1009" mass="113198" checksum="2018E18DF7C82AD8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPV8</accession>
    <name>A0A452IPV8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IPV8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029985"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034053.1">
        <property type="protein sequence ID" value="ENSGAGP00000029985.1"/>
        <property type="gene ID" value="ENSGAGG00000021647.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000055">
        <property type="entry name" value="Homeobox protein Hox-A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050296">
        <property type="entry name" value="Antp_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017995">
        <property type="entry name" value="Homeobox_antennapedia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00025">
        <property type="entry name" value="ANTENNAPEDIA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="188"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="190"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="61"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="114"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="265" mass="29375" checksum="58920CC6F8E80B0D" modified="2019-05-08" version="1">MSSYFVNSFSGRYPNGPDYQLLNYGTSSSMNGSYRDSSTMHSSSYGYNYNGMDLSINRSTSSSHFGAVGESSRSFPSPAQESRFRQASSCSLSSPESLPCSNSDNHGGKPAPSPSDQATSASTNTNFTEIDETSASSGTEEGTQINSSNISRAQAEPVATSTAATEGQTPQIFPWMRKLHISHDMTGPDGKRARTAYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLSERQIKIWFQNRRMKWKKDNKLKSMSVASAGSAFQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQW8</accession>
    <name>A0A452IQW8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RPAP1 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000030366"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034463.1">
        <property type="protein sequence ID" value="ENSGAGP00000030366.1"/>
        <property type="gene ID" value="ENSGAGG00000021786.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039913">
        <property type="entry name" value="RPAP1/Rba50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013929">
        <property type="entry name" value="RPAP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013930">
        <property type="entry name" value="RPAP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057989">
        <property type="entry name" value="TPR_RPAP1/MINIYO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21483">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21483:SF18">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08620">
        <property type="entry name" value="RPAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08621">
        <property type="entry name" value="RPAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25766">
        <property type="entry name" value="TPR_RPAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RPAP1 N-terminal" evidence="7">
        <location>
            <begin position="190"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="RPAP1 C-terminal" evidence="6">
        <location>
            <begin position="324"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="RPAP1/MINIYO-like TPR repeats" evidence="8">
        <location>
            <begin position="1076"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="229"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1373" mass="154285" checksum="6CB7DAFA9B694909" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IS48</accession>
    <name>A0A452IS48_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein MAK16 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MAK16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IS48"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030845"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034981.1">
        <property type="protein sequence ID" value="ENSGAGP00000030845.1"/>
        <property type="gene ID" value="ENSGAGG00000022177.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.110:FF:000003">
        <property type="entry name" value="Protein MAK16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006958">
        <property type="entry name" value="Mak16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029004">
        <property type="entry name" value="Ribosomal_eL28/Mak16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405">
        <property type="entry name" value="MAINTENANCE OF KILLER 16 MAK16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405:SF4">
        <property type="entry name" value="PROTEIN MAK16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04874">
        <property type="entry name" value="Mak16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01778">
        <property type="entry name" value="Ribosomal_L28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003352">
        <property type="entry name" value="MAK16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal eL28/Mak16" evidence="6">
        <location>
            <begin position="6"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="190"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="238"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="261"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR003352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="298" mass="35388" checksum="1D93311A21258E66" modified="2019-05-08" version="1">MHSDDIVWDSLGNKQFCSYKIKTKTHNFCRNEYNLTGLCNRSSCPLANSQYATIKEEKGQCYLYMKTIERAAFPSRLWERVRLSKNYEKALEEIDENLIYWPRFIRHKCKQRFTKITQYLIRIRKLTLKRQRKIVPLSKKVERREKRREEKALIAAQLDNAIEKELLERLKQETYGDIYNFPIHAFDKALEQQDAESKAESDTEEEEEDEDADKREFVEEDESDLSDFEDMEKLATSSEEEQDDKSSSEEEEKEMAERTYHSKSKGKTPVKGPARIKRAYIEIEYEQETEPTSKQKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITY2</accession>
    <name>A0A452ITY2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">E3 ubiquitin/ISG15 ligase TRIM25-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ITY2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031518"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035744.1">
        <property type="protein sequence ID" value="ENSGAGP00000031518.1"/>
        <property type="gene ID" value="ENSGAGG00000022637.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13737">
        <property type="entry name" value="SPRY_PRY_TRIM25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF41">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="46"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="8">
        <location>
            <begin position="180"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="9">
        <location>
            <begin position="398"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="67322" checksum="ACACC36F62959E7F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUB9</accession>
    <name>A0A452IUB9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000031490"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035714.1">
        <property type="protein sequence ID" value="ENSGAGP00000031490.1"/>
        <property type="gene ID" value="ENSGAGG00000022616.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000035717.1">
        <property type="protein sequence ID" value="ENSGAGP00000031493.1"/>
        <property type="gene ID" value="ENSGAGG00000022616.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071391">
        <property type="term" value="P:cellular response to estrogen stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032355">
        <property type="term" value="P:response to estradiol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.230.10">
        <property type="entry name" value="Lipovitellin, beta-sheet shell regions, chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.80.10">
        <property type="entry name" value="Lipovitellin-phosvitin complex, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.50.20">
        <property type="entry name" value="Lipovitellin. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.90.10">
        <property type="entry name" value="Vitellinogen, beta-sheet shell domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.20">
        <property type="entry name" value="Vitellinogen, superhelical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015819">
        <property type="entry name" value="Lipid_transp_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011030">
        <property type="entry name" value="Lipovitellin_superhlx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015816">
        <property type="entry name" value="Vitellinogen_b-sht_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015258">
        <property type="entry name" value="Vitellinogen_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037088">
        <property type="entry name" value="Vitellinogen_b-sht_shell_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015255">
        <property type="entry name" value="Vitellinogen_open_b-sht"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015817">
        <property type="entry name" value="Vitellinogen_open_b-sht_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050733">
        <property type="entry name" value="Vitellogenin/Apolipophorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001747">
        <property type="entry name" value="Vitellogenin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345:SF15">
        <property type="entry name" value="VITELLOGENIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345">
        <property type="entry name" value="VITELLOGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09175">
        <property type="entry name" value="Vit_b-sht_shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09172">
        <property type="entry name" value="Vit_open_b-sht"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01347">
        <property type="entry name" value="Vitellogenin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01169">
        <property type="entry name" value="DUF1943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01170">
        <property type="entry name" value="DUF1944"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00638">
        <property type="entry name" value="LPD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56968">
        <property type="entry name" value="Lipovitellin-phosvitin complex, beta-sheet shell regions"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48431">
        <property type="entry name" value="Lipovitellin-phosvitin complex, superhelical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51211">
        <property type="entry name" value="VITELLOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0758">Storage protein</keyword>
    <feature type="domain" description="Vitellogenin" evidence="8">
        <location>
            <begin position="1"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="9">
        <location>
            <begin position="1495"/>
            <end position="1673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="971"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="984"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1012"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1042"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1052"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1071"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1108"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1129"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1159"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1179"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1191"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1205"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1228"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1769" mass="195454" checksum="25D123C81C91DC16" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWX9</accession>
    <name>A0A452IWX9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">GPN-loop GTPase 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 8">Small GTPase required for proper localization of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimers with GPN1 or GPN3. Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IWX9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032560"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036908.1">
        <property type="protein sequence ID" value="ENSGAGP00000032560.1"/>
        <property type="gene ID" value="ENSGAGG00000023287.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17871">
        <property type="entry name" value="GPN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000338">
        <property type="entry name" value="GPN-loop GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030231">
        <property type="entry name" value="Gpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF3">
        <property type="entry name" value="GPN-LOOP GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="311" mass="35052" checksum="006B179CB2CDCA54" modified="2019-05-08" version="1">MSENHMSSLAFGQAVIGPPGSGKTTYCFGMQEFMSRIGRKVAVVNLDPANEGTPYQCAVDISELITLADVMDNLKLGPNGGLIYCMEYLETNFDWLQEKLAKLKGHYFLFDCPGQVELCTHHGALKNVFAQLAKWNFRLAAVHLVDSHYCTDPGKFISVLCTSLSTMLHVELPHVNVLSKMDLIEQYGKLAFNLDYYTEVLDLSYLVDHLAIDPFFKNYRRLNEKLVEVIEDYSLVSFVPLNVQDKESMRRVMQAVDKANGYSFGDLEQRSLEALMSAAVGADFHFTSTLAVQEKYVQPQEKTVEQEAMEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IX44</accession>
    <name>A0A452IX44_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 DOCK-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IX44"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032503"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036850.1">
        <property type="protein sequence ID" value="ENSGAGP00000032503.1"/>
        <property type="gene ID" value="ENSGAGG00000023250.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021816">
        <property type="entry name" value="DOCK_C/D_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF65">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11878">
        <property type="entry name" value="DOCK_C-D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 DOCK-type" evidence="3">
        <location>
            <begin position="542"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="147"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="162"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="639" mass="72105" checksum="1B6C2B15E029F67B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0L6</accession>
    <name>A0A452J0L6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sperm acrosome membrane-associated protein 3</fullName>
            <ecNumber evidence="5">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has strong bacteriolytic activity against M.luteus and V.cholerae, weak bacteriolytic activity against P.aeruginosa and no activity against A.hydrophila.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with ASTL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyl hydrolase 22 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452J0L6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033940"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038436.1">
        <property type="protein sequence ID" value="ENSGAGP00000033940.1"/>
        <property type="gene ID" value="ENSGAGG00000024132.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007342">
        <property type="term" value="P:fusion of sperm to egg plasma membrane involved in single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16897">
        <property type="entry name" value="LYZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000001">
        <property type="entry name" value="Lysozyme C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001916">
        <property type="entry name" value="Glyco_hydro_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000974">
        <property type="entry name" value="Glyco_hydro_22_lys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11407">
        <property type="entry name" value="LYSOZYME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11407:SF25">
        <property type="entry name" value="SPERM ACROSOME MEMBRANE-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00062">
        <property type="entry name" value="Lys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00137">
        <property type="entry name" value="LYSOZYME"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00135">
        <property type="entry name" value="LYZLACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00263">
        <property type="entry name" value="LYZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51348">
        <property type="entry name" value="GLYCOSYL_HYDROL_F22_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="9" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019328721" description="Sperm acrosome membrane-associated protein 3" evidence="16">
        <location>
            <begin position="20"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="127" mass="14275" checksum="25FC86B609169454" modified="2019-05-08" version="1">MAAFVLLSILACVLVGNMGKIFRRCELAQVLHQAGMDGFRGYNLADWLCMAFHESRFNTDMVDHEADGSTDNGIFQINSRQWCDDYRSSTRNLCHMHCSGDGKRVGENSSIFSVLQSDSKFSIMKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1F3</accession>
    <name>A0A452J1F3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Calcitonin peptide-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcitonin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J1F3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034217"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038744.1">
        <property type="protein sequence ID" value="ENSGAGP00000034217.1"/>
        <property type="gene ID" value="ENSGAGG00000024303.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031716">
        <property type="term" value="F:calcitonin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021118">
        <property type="entry name" value="Calcitonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021117">
        <property type="entry name" value="Calcitonin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021116">
        <property type="entry name" value="Calcitonin/adrenomedullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018360">
        <property type="entry name" value="Calcitonin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001693">
        <property type="entry name" value="Calcitonin_peptide-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10505:SF16">
        <property type="entry name" value="CALCITONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10505">
        <property type="entry name" value="CALCITONIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00214">
        <property type="entry name" value="Calc_CGRP_IAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00270">
        <property type="entry name" value="CALCITONINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00113">
        <property type="entry name" value="CALCITONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00258">
        <property type="entry name" value="CALCITONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0027">Amidation</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Calcitonin peptide-like" evidence="10">
        <location>
            <begin position="114"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="116"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR621116-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="172" mass="19389" checksum="01F25A5B46588A8F" modified="2019-05-08" version="1">MYLEKDCIFSRSVKIEEAGKWPLFDVTLCCREDIMVMLKISPFLAVYALVMCQMDSSQATPLRPGLDSITDRVTLSDYEARRLLNALVKEFVQMRAEDVEQETEGNSLDKPISKRCASLSTCVLGKLSQELHKLQTYPRTDVGAGTPGKKRNVLNDLENERYANYGESLGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1U3</accession>
    <name>A0A452J1U3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sclerostin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with LRP4 (via the extracellular domain); the interaction facilitates the inhibition of Wnt signaling. Interacts with LRP5 (via the first two YWTD-EGF repeat domains); the interaction inhibits Wnt-mediated signaling. Interacts with LRP6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sclerostin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J1U3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034264"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038798.1">
        <property type="protein sequence ID" value="ENSGAGP00000034264.1"/>
        <property type="gene ID" value="ENSGAGG00000024346.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036122">
        <property type="term" value="F:BMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008835">
        <property type="entry name" value="Sclerostin/SOSTDC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14903:SF4">
        <property type="entry name" value="SCLEROSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14903">
        <property type="entry name" value="SCLEROSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05463">
        <property type="entry name" value="Sclerostin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 14" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019460958" description="Sclerostin" evidence="14">
        <location>
            <begin position="24"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="15">
        <location>
            <begin position="80"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="38"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="180"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="213" mass="24552" checksum="1EE29F9804022139" modified="2019-05-08" version="1">MQISWTVCSICVLIQIAVHSVEAWQVFKNDATEIIPEIPENTETPVEQTNSNNNTMNQAKHGGRQPQPSLEPNDASDFSCRELRYTRYVIDGPCRSFKPIKELVCSGQCFPSHLLPNSIGRGKWWRQNALDYRCIPAHARTQRIQLACPQEETRTYKIRAVTACKCKRYTRYHNQSELKDFGKETIRPQKNKKPRLSRARGSKSNQPELENAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4X6</accession>
    <name>A0A452J4X6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">General transcription factor IIH subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Basic transcription factor 2 62 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">General transcription factor IIH polypeptide 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">TFIIH basal transcription factor complex p62 subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Interacts with PUF60.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J4X6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035533"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040241.1">
        <property type="protein sequence ID" value="ENSGAGP00000035533.1"/>
        <property type="gene ID" value="ENSGAGG00000025261.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000115">
        <property type="entry name" value="General transcription factor IIH subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="15">
        <location>
            <begin position="99"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="BSD" evidence="15">
        <location>
            <begin position="179"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="548" mass="62313" checksum="277936678864251A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J539</accession>
    <name>A0A452J539_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2 AKAP95-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AKAP95 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J539"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035574"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040286.1">
        <property type="protein sequence ID" value="ENSGAGP00000035574.1"/>
        <property type="gene ID" value="ENSGAGG00000025278.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000040287.1">
        <property type="protein sequence ID" value="ENSGAGP00000035575.1"/>
        <property type="gene ID" value="ENSGAGG00000025278.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000040289.1">
        <property type="protein sequence ID" value="ENSGAGP00000035577.1"/>
        <property type="gene ID" value="ENSGAGG00000025278.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007071">
        <property type="entry name" value="AKAP95"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034736">
        <property type="entry name" value="ZF_C2H2_AKAP95"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12190:SF4">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 8-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12190">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN AKAP 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04988">
        <property type="entry name" value="AKAP95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51799">
        <property type="entry name" value="ZF_C2H2_AKAP95"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2 AKAP95-type" evidence="9">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2 AKAP95-type" evidence="9">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="157"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="246"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="513"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="283"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="517"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="542"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="556"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="606"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="654" mass="74260" checksum="64D568DB1E7BB758" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J573</accession>
    <name>A0A452J573_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="9">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452J573"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035605"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040319.1">
        <property type="protein sequence ID" value="ENSGAGP00000035605.1"/>
        <property type="gene ID" value="ENSGAGG00000025292.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06617">
        <property type="entry name" value="PKc_MKK3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000126">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000158">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF12">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="53"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="394" mass="44598" checksum="7B151A3F90A4B08F" modified="2019-05-08" version="1">MSQPRGKKRNPGLKIPKEAFEQPQTSSTPPRDLDSKACISIGEKNFEVKADDLEPISELGRGAYGVVEKMRHMPSAQIMAVKRIRATVNSQEQKRLLMDLDISMRTVDCHFTVTFYGALFREGDVWICMELMDTSLDKFYKHVIDKGLTIPEDILGKIAVSIVKALEHLHSKLSVIHRDVKPSNVLINTQGQVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEPSPQLPADKFSAEFVDFTSQWWLPAREDSEMHVPQALHISSSHCFPVHGHLEEEFQRTANVPRAYATPFLHPARIQRDRRGFFCKTHPGRLRNGLVHDLPFCGLVRLGEEVCGGAWSRNHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J6M9</accession>
    <name>A0A452J6M9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J6M9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036150"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040927.1">
        <property type="protein sequence ID" value="ENSGAGP00000036150.1"/>
        <property type="gene ID" value="ENSGAGG00000025622.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF33">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="7">
        <location>
            <begin position="17"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="103"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="268" mass="28614" checksum="7B3578A24F40F3DF" modified="2019-05-08" version="1">SPAPHSHQPSPPSHARQGPITAQVRPLTCLERRRGAQRVVHCLGDNTVYVSAPRWTGQSWAAFRLSAVFDAGTSQEGVFEGSGMKRLIELAANGFSCTAFAFGQTGSGKTYTLMGSSAQSKGQPATPYLGGLMQRAFVCLLEQTQPYRPGLILSASYVEIYNEQVRDLLSPGPPRPLPVRWSKTRGFYIENLLVVEFGSLEAIMDLLQEGTAQGVSSLAGRWGGAQAGPPTGLCSPWARSPVQKGTPGGQVGSEFPFAAPSGWELLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GER6</accession>
    <name>A0A452GER6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GER6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000019"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000020.1">
        <property type="protein sequence ID" value="ENSGAGP00000000019.1"/>
        <property type="gene ID" value="ENSGAGG00000000010.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008484">
        <property type="term" value="F:sulfuric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16029">
        <property type="entry name" value="4-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000007">
        <property type="entry name" value="Arylsulfatase family, member J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047115">
        <property type="entry name" value="ARSB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342:SF274">
        <property type="entry name" value="ARYLSULFATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019119368" description="Sulfatase N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="31"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="8">
        <location>
            <begin position="32"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="522" mass="58820" checksum="10F444075CD23DEB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFC5</accession>
    <name>A0A452GFC5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 7">BH3-interacting domain death agonist</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Induces caspases and apoptosis. Counters the protective effect of BCL2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GFC5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000264"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000274.1">
        <property type="protein sequence ID" value="ENSGAGP00000000241.1"/>
        <property type="gene ID" value="ENSGAGG00000000215.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000000300.1">
        <property type="protein sequence ID" value="ENSGAGP00000000264.1"/>
        <property type="gene ID" value="ENSGAGG00000000215.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001238">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001244">
        <property type="term" value="P:positive regulation of intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090200">
        <property type="term" value="P:positive regulation of release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.437.10:FF:000010">
        <property type="entry name" value="BH3-interacting domain death agonist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010479">
        <property type="entry name" value="BID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35447">
        <property type="entry name" value="BH3-INTERACTING DOMAIN DEATH AGONIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35447:SF1">
        <property type="entry name" value="BH3-INTERACTING DOMAIN DEATH AGONIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06393">
        <property type="entry name" value="BID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038018">
        <property type="entry name" value="BID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00015802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="188" mass="21200" checksum="F0577D63C542186E" modified="2019-05-08" version="1">MDQGLRGDVQVECILVYSFLKNCHNCDFSKELDSLRSQVMVSSAKDFLCVESDDGELQTDGNRSGRFQNGEPGSGFDEDVFQLIGAQLAEIGDQLAREIDQRVVNDLVLQFVNENLSRAEIMRRLSNTVEGLMRTVPLEMEREKAMLVLAMVLARTVANKMPSLLHRVFNTTVNYINQNLHNYVINLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGD0</accession>
    <name>A0A452GGD0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Urokinase plasminogen activator surface receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GGD0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000636"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000714.1">
        <property type="protein sequence ID" value="ENSGAGP00000000636.1"/>
        <property type="gene ID" value="ENSGAGG00000000521.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23556">
        <property type="entry name" value="TFP_LU_ECD_uPAR_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23558">
        <property type="entry name" value="TFP_LU_ECD_uPAR_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000015">
        <property type="entry name" value="Urokinase plasminogen activator surface receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018363">
        <property type="entry name" value="CD59_antigen_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10624:SF6">
        <property type="entry name" value="UROKINASE PLASMINOGEN ACTIVATOR SURFACE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10624">
        <property type="entry name" value="UROKINASE PLASMINOGEN ACTIVATOR SURFACE RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00021">
        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00134">
        <property type="entry name" value="LU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00983">
        <property type="entry name" value="LY6_UPAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019489814" description="Urokinase plasminogen activator surface receptor" evidence="13">
        <location>
            <begin position="21"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="14">
        <location>
            <begin position="21"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="14">
        <location>
            <begin position="112"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="14">
        <location>
            <begin position="211"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="332" mass="35289" checksum="081D858E8CE04713" modified="2019-05-08" version="1">GGWDSLVLFQFLRVSPPAAALRCYSCDGARSCQETEDCGEQHGRCRTTVLTMSSEVSKRIQKGCDVEGKPNNSISFLSHGQIVTLAEEQCATDLCNQGAPDALSTFLSTSSLHCLSCSSSDHSCSSPSLMGIRCQDPREQCVDITAISMPEEFPQDERRIKGCGQISQCQEPLGFHNQGSFYLLQCCNTSLCNNDAHGNPGTESPLPLNGVTCYSCEGNSSHGCAPENVTPVQCQGPMTHCLQAVGKLTGPGTVLKGCATPAWCDAPYTSIYKRLAGVQAHCCHGNFCNSWIQAGALPPSRRSRAGHRLVAQPALLCGAALLAVLILLPSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GH66</accession>
    <name>A0A452GH66_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear factor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Binds DNA as a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the CTF/NF-I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GH66"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001072.1">
        <property type="protein sequence ID" value="ENSGAGP00000000947.1"/>
        <property type="gene ID" value="ENSGAGG00000000774.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000647">
        <property type="entry name" value="CTF/NFI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020604">
        <property type="entry name" value="CTF/NFI_DNA-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019739">
        <property type="entry name" value="CTF/NFI_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019548">
        <property type="entry name" value="CTF/NFI_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11492:SF3">
        <property type="entry name" value="NUCLEAR FACTOR 1 X-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11492">
        <property type="entry name" value="NUCLEAR FACTOR I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00859">
        <property type="entry name" value="CTF_NFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10524">
        <property type="entry name" value="NfI_DNAbd_pre-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00349">
        <property type="entry name" value="CTF_NFI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51080">
        <property type="entry name" value="CTF_NFI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0010">Activator</keyword>
    <keyword evidence="3 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CTF/NF-I" evidence="11">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="264"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="402" mass="44921" checksum="7EBCC4618C7048BC" modified="2019-05-08" version="1">MYSPYCLTQDEFHPFIEALLPHVRAFSYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEIKQKWASRLLAKLRKDIRPEFREDFVLTITGKKAPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLYKSPQCSNPGLCVQPHHIGVTIKELDLYLAYFVHTPESGQSDSSNQQGDVDIKPLPNGHLTFQDCFVTSGVWNVTELVRVSQTPVATASGPNFSLADLESPSYYNINQVTLGRRSITSPPSTSSTKRPKSLDDSEMESPVDEVFYPGTGRSPAAGSSQSSGWPNDVDAGPASLKKSGKLDFCSALSTHSTSPRMAFSHHPLPVLAGVRPGENQSRYPGGKGGQGPCPSKPQWFWQLISRFSSLSLSLLFRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GI80</accession>
    <name>A0A452GI80_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GI80"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001278"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001436.1">
        <property type="protein sequence ID" value="ENSGAGP00000001278.1"/>
        <property type="gene ID" value="ENSGAGG00000001026.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02123">
        <property type="entry name" value="PA_C_RZF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044744">
        <property type="entry name" value="ZNRF4/RNF13/RNF167_PA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PA" evidence="8">
        <location>
            <begin position="29"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="144" mass="15434" checksum="C74AD0B9CC133E02" modified="2019-05-08" version="1">MVSVHPHNFRALSACLGPPVPREGLRGYLIEAAPANACHPIGGPPAGGNSSAAFIVLISRYDCPLGVKILHAQQAGYQAAIVHNVNSEKLVTMVTNVEEIRQKVGIPSVFTGESASKQLRRVMGSEKGIHRRPIRNVCDLHGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIV9</accession>
    <name>A0A452GIV9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GIV9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001540"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001756.1">
        <property type="protein sequence ID" value="ENSGAGP00000001540.1"/>
        <property type="gene ID" value="ENSGAGG00000001234.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01381">
        <property type="entry name" value="MYSc_Myo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000008">
        <property type="entry name" value="Putative unconventional myosin-IXa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.530">
        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057130">
        <property type="entry name" value="Myosin_VII_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036106">
        <property type="entry name" value="MYSc_Myo7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051567">
        <property type="entry name" value="Unconventional_Myosin_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692">
        <property type="entry name" value="MYOSIN VII, XV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692:SF24">
        <property type="entry name" value="MYOSIN VIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24123">
        <property type="entry name" value="Myosin_VII_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="11">
        <location>
            <begin position="66"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="10">
        <location>
            <begin position="936"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="596"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="159"/>
            <end position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1102" mass="125547" checksum="FAE9267383C532B9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJ18</accession>
    <name>A0A452GJ18_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription factor AP-2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GJ18"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001596"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001816.1">
        <property type="protein sequence ID" value="ENSGAGP00000001596.1"/>
        <property type="gene ID" value="ENSGAGG00000001284.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004979">
        <property type="entry name" value="TF_AP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013854">
        <property type="entry name" value="TF_AP2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03299">
        <property type="entry name" value="TF_AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01748">
        <property type="entry name" value="AP2TNSCPFCT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor AP-2 C-terminal" evidence="8">
        <location>
            <begin position="223"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="427"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="448" mass="49220" checksum="B4D81E311E1B8E0D" modified="2019-05-08" version="1">MLWKLADNVKYEEDCEDRHDVSSNGNPRLPHLSAVSQHLYSPAPQLSHSGTSDFQPPYFPPPYQPLPYSQSSDPYSHLGDPFSINPLHQPPPPPPSQQQAAWPSRQSQDPTGLVHHARPGLVPHLSALESGSAGGRRETYRRSELLLPHGHGLDASTLAENLGLHEMAHQMEEVPNVEDQHLLMHDQTVIRKGPISLTKNSSLSLPCQKDGLIGVVINPNEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYVCETEFPSKAVAEYLTRPHLGRNEMANRKNMLLAAKQICKEFTDLLTQDRTPLGNTRPSPILDPGIQGCLTHFSLITHGFGSAAICAAMTSVQNYLNEALKIADKTYMNTGDQSPAETNKNIDKMDKHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GK17</accession>
    <name>A0A452GK17_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ODAD1 central coiled coil region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GK17"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001932"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002199.1">
        <property type="protein sequence ID" value="ENSGAGP00000001932.1"/>
        <property type="gene ID" value="ENSGAGG00000001553.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051876">
        <property type="entry name" value="ODA-DC/CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049258">
        <property type="entry name" value="ODAD1_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21694">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21694:SF35">
        <property type="entry name" value="OUTER DYNEIN ARM-DOCKING COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21773">
        <property type="entry name" value="ODAD1_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ODAD1 central coiled coil region" evidence="4">
        <location>
            <begin position="126"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="471"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="37"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="318"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="481"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="492"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="539" mass="61596" checksum="8AE78F7598631BE8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKP4</accession>
    <name>A0A452GKP4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged tRNA(Thr) via its editing domain, at the post-transfer stage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452GKP4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002168"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002474.1">
        <property type="protein sequence ID" value="ENSGAGP00000002168.1"/>
        <property type="gene ID" value="ENSGAGG00000001743.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01667">
        <property type="entry name" value="TGS_ThrRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000009">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000006">
        <property type="entry name" value="Threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000003">
        <property type="entry name" value="Threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF27">
        <property type="entry name" value="THREONINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGS" evidence="15">
        <location>
            <begin position="59"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="327"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="672"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="708" mass="79974" checksum="F33D6071F4C88055" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMF5</accession>
    <name>A0A452GMF5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRELI/MSF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GMF5"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003433.1">
        <property type="protein sequence ID" value="ENSGAGP00000002997.1"/>
        <property type="gene ID" value="ENSGAGG00000002393.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="176" mass="20320" checksum="36DF938BE892F6F4" modified="2019-05-08" version="1">MGVTVDVHQVFRYPFEQVVASYLRKYPSPMEKHVTAIKTMEEKTDLSTGIIYRRRIATCQNVIPEILRKVSVLKVSDIYLEEESWLNMQERNMVMKSHCLTWTQYASLSEESVFRESLENPNWTEFIQKGRISITGAGLLNCVLEAFAQTFLNQGVKKGIRIMETLLQEQCGCPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNJ4</accession>
    <name>A0A452GNJ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ENR1 protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GNJ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003309"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003813.1">
        <property type="protein sequence ID" value="ENSGAGP00000003309.1"/>
        <property type="gene ID" value="ENSGAGG00000002673.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018154">
        <property type="entry name" value="TLV/ENV_coat_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10424:SF68">
        <property type="entry name" value="ENDOGENOUS RETROVIRUS GROUP 3 MEMBER 1 ENV POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10424">
        <property type="entry name" value="VIRAL ENVELOPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58069">
        <property type="entry name" value="Virus ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11089" checksum="6D4A82EC61F12F6D" modified="2019-05-08" version="1">IEPIYNLNRIIRLQAVLEIITNQTAAALDLLADQSTQMRNAIYQHHMVLDYLLAEEGGVCAKLNESNCCIQIDDNGKAVKQLTKEMRKLAHVPVQTWGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQ54</accession>
    <name>A0A452GQ54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="3">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004042"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004716.1">
        <property type="protein sequence ID" value="ENSGAGP00000004042.1"/>
        <property type="gene ID" value="ENSGAGG00000003312.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01179">
        <property type="entry name" value="IPT_plexin_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000679">
        <property type="entry name" value="Macrophage stimulating 1 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000251">
        <property type="entry name" value="Macrophage stimulating 1 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000194">
        <property type="entry name" value="Macrophage-stimulating 1 receptor a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000006">
        <property type="entry name" value="Macrophage-stimulating 1 receptor b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000554">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF564">
        <property type="entry name" value="MACROPHAGE-STIMULATING PROTEIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 22" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019051651" description="receptor protein-tyrosine kinase" evidence="22">
        <location>
            <begin position="21"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="966"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="24">
        <location>
            <begin position="27"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="1086"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="1118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1320" mass="146063" checksum="0CDCA8DC9EED438C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GS27</accession>
    <name>A0A452GS27_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15 20">Postsynaptic cell membrane</location>
            <topology evidence="15 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 20">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GS27"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004805"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005624.1">
        <property type="protein sequence ID" value="ENSGAGP00000004805.1"/>
        <property type="gene ID" value="ENSGAGG00000003947.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13685">
        <property type="entry name" value="PBP2_iGluR_non_NMDA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000143">
        <property type="entry name" value="Probable glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000364">
        <property type="entry name" value="Si:dkey-183j2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 20" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 20" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 20" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0732">Signal</keyword>
    <keyword evidence="7 20" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 20" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027162920" description="Glutamate receptor" evidence="20">
        <location>
            <begin position="27"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="21">
        <location>
            <begin position="49"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="22">
        <location>
            <begin position="59"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="18">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="18">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="18">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="352"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="529" mass="58432" checksum="B621BF471B0C705E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSR2</accession>
    <name>A0A452GSR2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GSR2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005013"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005851.1">
        <property type="protein sequence ID" value="ENSGAGP00000005013.1"/>
        <property type="gene ID" value="ENSGAGG00000003169.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13969">
        <property type="entry name" value="ADCK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045307">
        <property type="entry name" value="ADCK1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF28">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019398968" description="ABC1 atypical kinase-like domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="3">
        <location>
            <begin position="127"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="537" mass="61350" checksum="2E8EA6A772834691" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GT21</accession>
    <name>A0A452GT21_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein arginine N-methyltransferase 3</fullName>
            <ecNumber evidence="3">2.1.1.319</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:48097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452GT21"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005044"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005884.1">
        <property type="protein sequence ID" value="ENSGAGP00000005044.1"/>
        <property type="gene ID" value="ENSGAGG00000004116.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000034">
        <property type="entry name" value="Protein arginine N-methyltransferase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000005">
        <property type="entry name" value="protein arginine N-methyltransferase 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049482">
        <property type="entry name" value="ANM3-like_C2H2_Zf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049009">
        <property type="entry name" value="ANM3_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF53">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21137">
        <property type="entry name" value="ANM3_C2H2_Zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21336">
        <property type="entry name" value="ANM3_zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 18" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00075283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="526" mass="58867" checksum="708752F9225E5E2D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GT94</accession>
    <name>A0A452GT94_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GT94"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005240"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006102.1">
        <property type="protein sequence ID" value="ENSGAGP00000005240.1"/>
        <property type="gene ID" value="ENSGAGG00000004252.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="20"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="107" mass="11878" checksum="4A5EB910B0375860" modified="2019-05-08" version="1">MELDKDRCIKMNDGNKIPALGFGTYCPDAVQKSKCEEATKVAIEVGFRHIDGAFVYGIEEEVGRAVHEKIADGTVKREDIFYTGKVSRFCFNTAASFRAESCSQTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTA7</accession>
    <name>A0A452GTA7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTA7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004949"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005779.1">
        <property type="protein sequence ID" value="ENSGAGP00000004949.1"/>
        <property type="gene ID" value="ENSGAGG00000004050.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11469">
        <property type="entry name" value="bHLH-PAS_ARNTL2_PASD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000006">
        <property type="entry name" value="aryl hydrocarbon receptor nuclear translocator-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000018">
        <property type="entry name" value="Aryl hydrocarbon receptor nuclear translocator-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000010">
        <property type="entry name" value="Aryl hydrocarbon receptor nuclear translocator-like, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050933">
        <property type="entry name" value="Circadian_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001067">
        <property type="entry name" value="Nuc_translocat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23042">
        <property type="entry name" value="CIRCADIAN PROTEIN CLOCK/ARNT/BMAL/PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00785">
        <property type="entry name" value="NCTRNSLOCATR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="11">
        <location>
            <begin position="85"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="10">
        <location>
            <begin position="156"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="10">
        <location>
            <begin position="356"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="461"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="617" mass="68787" checksum="968C15A0D3B9C2FF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GU85</accession>
    <name>A0A452GU85_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MHC class I-like antigen recognition-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GU85"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005603"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006531.1">
        <property type="protein sequence ID" value="ENSGAGP00000005603.1"/>
        <property type="gene ID" value="ENSGAGG00000004520.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030883">
        <property type="term" value="F:endogenous lipid antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030884">
        <property type="term" value="F:exogenous lipid antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071723">
        <property type="term" value="F:lipopeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048006">
        <property type="term" value="P:antigen processing and presentation, endogenous lipid antigen via MHC class Ib"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048007">
        <property type="term" value="P:antigen processing and presentation, exogenous lipid antigen via MHC class Ib"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001916">
        <property type="term" value="P:positive regulation of T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF160">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16497">
        <property type="entry name" value="MHC_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHC class I-like antigen recognition-like" evidence="2">
        <location>
            <begin position="25"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="230" mass="25348" checksum="C1BC6551A5D782F8" modified="2019-05-08" version="1">DPPPALLLLPNSLCPDWLGLAAAGTLTLRLLKTTVYHNASSTNMEGMALLGDLGTHSMDCSTCKIRFLQPWAHQGLTPKQWQGLETVLHNYVSKFILLMNKVVKDEDEHYPFVLQASIGCELHPNGTSRQFCDAAVNGEDFISFSVDEGTWVAQRGDKLALSIRDLLNQDKSAAAVAQFLLRTTCINELKSFVQYGKESLERQGEAGCHSPLTEMQPLLGWCCQRLTPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUL7</accession>
    <name>A0A452GUL7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Platelet-derived growth factor subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">PDGF-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Platelet-derived growth factor B chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Platelet-derived growth factor beta polypeptide</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Antiparallel homodimer; disulfide-linked. Antiparallel heterodimer with PDGFA; disulfide-linked. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1. Interacts with LRP1. Interacts with SORL1 (via the N-terminal ectodomain). Interacts with CD82; this interaction inhibits PDGFB-mediated signaling pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the PDGF/VEGF growth factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GUL7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005454"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006349.1">
        <property type="protein sequence ID" value="ENSGAGP00000005454.1"/>
        <property type="gene ID" value="ENSGAGG00000004425.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005161">
        <property type="term" value="F:platelet-derived growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048008">
        <property type="term" value="P:platelet-derived growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014911">
        <property type="term" value="P:positive regulation of smooth muscle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048661">
        <property type="term" value="P:positive regulation of smooth muscle cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00135">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000023">
        <property type="entry name" value="Platelet-derived growth factor subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023581">
        <property type="entry name" value="PD_growth_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000072">
        <property type="entry name" value="PDGF/VEGF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006782">
        <property type="entry name" value="PDGF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11633">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11633:SF2">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00341">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04692">
        <property type="entry name" value="PDGF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00141">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00249">
        <property type="entry name" value="PDGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50278">
        <property type="entry name" value="PDGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0339">Growth factor</keyword>
    <keyword evidence="4" id="KW-0497">Mitogen</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018983034" description="Platelet-derived growth factor subunit B" evidence="12">
        <location>
            <begin position="32"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Platelet-derived growth factor (PDGF) family profile" evidence="13">
        <location>
            <begin position="88"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="248" mass="28191" checksum="EC61900CBE40CA3C" modified="2019-05-08" version="1">MCLELLGLRVSMDFRLIFMSLWYLPLISTEGDPIPEDIYKIMSDSSVRSRSDLQRALQIDSVEEDGSNLDLNSTRAHPGQNLPRGRRSLVVDAEPAVLAECKTRTEVFEISRSMVDSTNANFVVWPPCVEVQRCSGCCNNRNVQCRPTLIRVRHVQVNKIEFVQKKPNFKKAIVPLEDHLECRCEALSSQPSGRGRPGSREHRGKSASITAAPVSVRQRVRQLPARKRKHRKFKHVHDKKVLKEILTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUS2</accession>
    <name>A0A452GUS2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein-tyrosine-phosphatase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GUS2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005845"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006821.1">
        <property type="protein sequence ID" value="ENSGAGP00000005845.1"/>
        <property type="gene ID" value="ENSGAGG00000004753.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020420">
        <property type="entry name" value="Atypical_DUSP_subfamB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46495:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PHOSPHATASE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46495">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01910">
        <property type="entry name" value="ADSPHPHTASEB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="14">
        <location>
            <begin position="19"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="15">
        <location>
            <begin position="81"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="21320" checksum="5507FC7C9C3F9788" modified="2025-10-08" version="2">MPHRGYLAPGAPPASALGGIAQISPCLYLCSGNAAANRHLVSARAVTCVVNATMEIPNANWPDIDYVKVPVPDLPHAPLALYFDSVADRIHQTGKRNGRTLVHCVAGVSRSASLCIAYLMKYHRLSLLDAHQWVKSRRPVVRPNVGFWRQLIDYERRLFGKNTVRMVPSPIGPVPDVYEKETRGLVPLWSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWY1</accession>
    <name>A0A452GWY1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mothers against decapentaplegic homolog</fullName>
            <shortName evidence="9">MAD homolog</shortName>
            <shortName evidence="9">Mothers against DPP homolog</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">SMAD family member</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the dwarfin/SMAD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GWY1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006619"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007694.1">
        <property type="protein sequence ID" value="ENSGAGP00000006619.1"/>
        <property type="gene ID" value="ENSGAGG00000005349.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060395">
        <property type="term" value="P:SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10490">
        <property type="entry name" value="MH1_SMAD_1_5_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10497">
        <property type="entry name" value="MH2_SMAD_1_5_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000001">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.520.10:FF:000001">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.520.10">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013790">
        <property type="entry name" value="Dwarfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013019">
        <property type="entry name" value="MAD_homology_MH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036578">
        <property type="entry name" value="SMAD_MH1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703:SF36">
        <property type="entry name" value="MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703">
        <property type="entry name" value="SMAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56366">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51075">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MH1" evidence="11">
        <location>
            <begin position="13"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="MH2" evidence="12">
        <location>
            <begin position="271"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="163"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="169"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="465" mass="52288" checksum="F9BAB855C1869133" modified="2019-05-08" version="1">MTSMASLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSSPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLDICEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEFNPQHSLLVQFRNLSHNEPHMPQNATFPDSFQQPNSTPFSISPNSPYPPSPVSSTYPSSPASSGPSSPFQLPADTPPPAYMPPEDHMGQDNSQSMDTSNTMIPQIMPNISSRDVQPVAYEEPKHWCSIMYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPLNPISSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXG7</accession>
    <name>A0A452GXG7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Deoxyribonuclease-1-like 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DNase X</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Deoxyribonuclease I-like 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNase I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXG7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006834"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007930.1">
        <property type="protein sequence ID" value="ENSGAGP00000006834.1"/>
        <property type="gene ID" value="ENSGAGG00000005510.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004530">
        <property type="term" value="F:deoxyribonuclease I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10282">
        <property type="entry name" value="DNase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016202">
        <property type="entry name" value="DNase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11371">
        <property type="entry name" value="DEOXYRIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11371:SF28">
        <property type="entry name" value="DEOXYRIBONUCLEASE-1-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000988">
        <property type="entry name" value="DNase_I_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00130">
        <property type="entry name" value="DNASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00476">
        <property type="entry name" value="DNaseIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019051924" description="Deoxyribonuclease-1-like 1" evidence="15">
        <location>
            <begin position="21"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="16">
        <location>
            <begin position="25"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Essential for enzymatic activity" evidence="14">
        <location>
            <begin position="193"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000988-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000988-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="334" mass="36351" checksum="FA5B4DA35DDDCAFE" modified="2019-05-08" version="1">MGLPLRTLLLLLLLCPAGAPFRICAFNLQRFASPKAATAAVMDTLVKIVSRCDIAVLQEVMDAKGQAVPALISALHRSAGPDSYTALGSPLLGAGNYQERYVFVYRSGCTQVLDSYVYLDENPDRPDAFAREPFVVHFSLPSKVLPTLVLVPQHTVPKKAEAEIDALYDVFLDVQARWGTEDIMFLGDFNADCGYVAKKRWGQIQLRREPGFHWLIGDTADTTVRNSTHCAYDRWARGGGGCSRDAGSCNPLPSSPCRIVVHGERCLGLVVPGSAQPFDFPAMFGLTETEALKVSDHYPVEVQLELNTAPRGHCPAGPALLPLAMLLARLGGWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYY6</accession>
    <name>A0A452GYY6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="19">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via interaction with STUB1/CHIP and promotion of STUB1-mediated ubiquitination and degradation of ICER-type isoforms of CREM. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="19">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A452GYY6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007264"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008401.1">
        <property type="protein sequence ID" value="ENSGAGP00000007264.1"/>
        <property type="gene ID" value="ENSGAGG00000005839.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07855">
        <property type="entry name" value="STKc_ERK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000013">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000242">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="49"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="416"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="499"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="607"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="661"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="857"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="512"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="537"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="685"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1044" mass="112094" checksum="1757170C281358A7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H016</accession>
    <name>A0A452H016_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H016"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007759"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008948.1">
        <property type="protein sequence ID" value="ENSGAGP00000007759.1"/>
        <property type="gene ID" value="ENSGAGG00000006192.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03156">
        <property type="entry name" value="uroplakin_I_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000012">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF249">
        <property type="entry name" value="TETRASPANIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="247" mass="27757" checksum="C9533A6602EB2739" modified="2019-05-08" version="1">MEGDCLSCMKYLMFVFNFFIFLGGACLLGVGIWVIVDPTGFREIVAANPLLFTGAYIMLAMGAMLFLLGFLGCCGAVRENKCLLLFFFMFILLIFLAELSAAILAFIFRENLTREFFTKELKKHYQGNNETDVFSSTWNSVMITFGCCGVNGPEDFETAPRFRLLHSDDVVPEACCQRELQTRDGAFVSKEDCLMGKDMYQNRQGCYTVILNSFETYVYLAGALAIGVLAIELFAMIFAMCLFRGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H126</accession>
    <name>A0A452H126_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L14</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H126"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008156"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009393.1">
        <property type="protein sequence ID" value="ENSGAGP00000008156.1"/>
        <property type="gene ID" value="ENSGAGG00000006480.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23702">
        <property type="entry name" value="eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000022">
        <property type="entry name" value="60S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_eL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002784">
        <property type="entry name" value="Ribosomal_eL14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01929">
        <property type="entry name" value="Ribosomal_L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL14" evidence="6">
        <location>
            <begin position="47"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="138" mass="16114" checksum="1C5CB51F03EC8DFF" modified="2019-05-08" version="1">MVFKRYVEIGRVAYISFGPHAGKLVAIVDVIDQNRALVDGPCSGVRRQAMPFKCMQLTDFVLKFPHSARQKHVRVAWEKENINEKWAGTRWAKKIEAREKKAKMTDFDRYKAMKAKKMRNRIIKHEVKKLQKQASKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H211</accession>
    <name>A0A452H211_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Conserved oligomeric Golgi complex subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Component of oligomeric Golgi complex 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COG4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H211"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008497"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009782.1">
        <property type="protein sequence ID" value="ENSGAGP00000008497.1"/>
        <property type="gene ID" value="ENSGAGG00000006755.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1060:FF:000002">
        <property type="entry name" value="Conserved oligomeric Golgi complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1970:FF:000001">
        <property type="entry name" value="Conserved oligomeric Golgi complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048682">
        <property type="entry name" value="COG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048684">
        <property type="entry name" value="COG4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013167">
        <property type="entry name" value="COG4_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048680">
        <property type="entry name" value="COG4_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20662">
        <property type="entry name" value="COG4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08318">
        <property type="entry name" value="COG4_m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20663">
        <property type="entry name" value="COG4_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00762">
        <property type="entry name" value="Cog4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="COG4 transport protein middle alpha-helical bundle" evidence="12">
        <location>
            <begin position="207"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="804" mass="90695" checksum="2162DE6705EBEDC9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H294</accession>
    <name>A0A452H294_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-dependent kinase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H294"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008575"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009864.1">
        <property type="protein sequence ID" value="ENSGAGP00000008575.1"/>
        <property type="gene ID" value="ENSGAGG00000006798.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072331">
        <property type="term" value="P:signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.365.10">
        <property type="entry name" value="p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003175">
        <property type="entry name" value="CDI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044898">
        <property type="entry name" value="CDI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029841">
        <property type="entry name" value="CDKN1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46778">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46778:SF2">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="4">
        <location>
            <begin position="43"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="182" mass="20818" checksum="135642B39D1D45A4" modified="2019-05-08" version="1">MPDVSDDLSISGRESLLCTLVGERWIGEQMEWRPVKANRVRRNLFGPVDHEQLQQDFQHMLHSSMEGAQQKWNFDFLRDVPAEGLLQWEELQSHDVPAFYHSCVVSEARKPLKPLNQGIVKEVKTHHFATVTLTENPKVAKKMSGKKSQAGKKRRQTSLTDYYSAKKQVKTNTQAAAKNLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2Q7</accession>
    <name>A0A452H2Q7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H2Q7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008908"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010235.1">
        <property type="protein sequence ID" value="ENSGAGP00000008908.1"/>
        <property type="gene ID" value="ENSGAGG00000007040.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000508">
        <property type="entry name" value="Myeloid zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000218">
        <property type="entry name" value="Zinc finger protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF163">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="224" mass="25687" checksum="09BF07FD4C108488" modified="2019-05-08" version="1">WVVDLQDSVDGAIPETDQETTCKGQHKSQKQKGNTARVRKPPVPERTHTGERPFSCPECGKHFSHFSNLTRHQRTHTGERPYSCPECEKRFSNLSSLTTHLRTHTGERPFTCPQCEKSFGDQSNLTTHLRTHTGERPYVCPDCQKSFSDQSTFAKHHRTHTGERPYPCPDCEKSFIHRSHLTTNHVGEGAYKCTDCDKIFSEQLDLVEHLKVHLAFHQGWEGHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7K1</accession>
    <name>A0A452H7K1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H7K1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010725"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012297.1">
        <property type="protein sequence ID" value="ENSGAGP00000010725.1"/>
        <property type="gene ID" value="ENSGAGG00000008365.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016312">
        <property type="term" value="F:inositol bisphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046627">
        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051898">
        <property type="term" value="P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09094">
        <property type="entry name" value="INPP5c_INPP5J-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2840:FF:000005">
        <property type="entry name" value="inositol polyphosphate 5-phosphatase K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000066">
        <property type="entry name" value="Inositol polyphosphate-5-phosphatase Kb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041611">
        <property type="entry name" value="SKICH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF117">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE 5-PHOSPHATASE K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17751">
        <property type="entry name" value="SKICH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="3">
        <location>
            <begin position="16"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="447"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="477" mass="54995" checksum="49F8869EE0B4188F" modified="2019-05-08" version="1">MEPRGLAPPIDLEIMKNLRLHLVTWNVGTASPPPDVTSLLQLSSQGPSMDMYVIGLQEVNSKIINFLSDLAFDDPWSIFFMTVFSPLGYIKLSSVRMQGLLLLVFVKHAHIPFIRDIHTHYTRTGLYGYWGNKGGVTIRMSLYGHMICFMNCHLPAHIENAEQRLDDFEKILEMQQFEDENVPNILDHDILFWFGDLNFRIADYGIHFVRESISNSRYNLLWEKDQLNMAKKKEAFLQEFIEGPLQFKPTYKFDLHSDVYDTRGQKTLFWFNGKKRKPAWTDRILWRVKNLSQHSSEDGDLFEGEQTISVTLNNYISHMSYGISDHKPVTGTFGLQIKPLFSTPLVTLNPEGEWNAAQDVLISYSTVSEFPSSTWDWIGLYQVTFRHVNDYVTYAWVKDDEISSSEDVTQVYISADEIPHAGGEFLLCYYSHNMQSIAGMSQPFQVQPSKRSAEKELAQENIDGIEKPHSPEPYNEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8B5</accession>
    <name>A0A452H8B5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tumor protein p53 inducible protein 13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H8B5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010794"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012366.1">
        <property type="protein sequence ID" value="ENSGAGP00000010794.1"/>
        <property type="gene ID" value="ENSGAGG00000008393.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34179">
        <property type="entry name" value="TUMOR PROTEIN P53-INDUCIBLE PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34179:SF1">
        <property type="entry name" value="TUMOR PROTEIN P53-INDUCIBLE PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="177"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="41608" checksum="B65BC4DBFB2F3949" modified="2019-05-08" version="1">MWHRLQQELSPSCGAASSAAWTRGLTLTSPPATGNGQAPAHPRGTVSSGPGLPWGAQWGRGALKGALQHWFCSLQWAPRAVSGQYTCCFPSLGSITSSTEGLPSSTPMHPPQPPGLARPAGPVHPDPPQQPHAGVGLAEAPQTPPLALWAGRPRAAQRAPRTLDKALLVPEGPLQPRSRISGSSRNRSGSPTPELAGASLTPGHSQAWGGVTLEPRGDRDRLDIAPPSGSSLSTRATAGQRSAPMLPSTDALPQASAARGGVSGPGALSPATLQHTPQLQEGPGHLGCSCSNAQGGAVPEGQAPQAYVPTPRTEEAAWAASALTFLLVVLTLAVLYTRLHRKFHKNQSLYWAPSDAGQEPLAALLRRRLLAAHGRHKKRQRPQQRRLLLQGPSSESSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAR3</accession>
    <name>A0A452HAR3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HAR3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011830"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013545.1">
        <property type="protein sequence ID" value="ENSGAGP00000011830.1"/>
        <property type="gene ID" value="ENSGAGG00000009114.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000005">
        <property type="entry name" value="Serine protease 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF90">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="24"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="126" mass="13247" checksum="068AE84FE0B76DE6" modified="2019-05-08" version="1">MELTHAVTGNGHKPLSASPVASAWVGAAFSLSGGMKPMISSVGVYCGGFLVAPDWVMTAAHCMGDISVILGAHNYHIPEASQQMFAVESYHPHPEYNANYVPYNDILLLKVTGLPNGERIPAPAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HB13</accession>
    <name>A0A452HB13_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HB13"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011925"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013661.1">
        <property type="protein sequence ID" value="ENSGAGP00000011925.1"/>
        <property type="gene ID" value="ENSGAGG00000009193.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052431">
        <property type="entry name" value="SKI2_subfamily_helicases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44533:SF3">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DDX60-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44533">
        <property type="entry name" value="DEAD/H RNA HELICASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="167" mass="18668" checksum="2D8104286FFC903B" modified="2019-05-08" version="1">MADMKQEYQLPLSRTDFSGEECNDSKLVSHLTSCNEGRTAVSPFACLSGNMDSDLLHAETVNSVILRTVGITAGNIPVLCAKKFDNRRRRMPLNAYALDFYKHGSLKAMAQDNGIHEGEAYLLLKDFSLTIKAISVSLRELCDDEEDNVVRAFSQLGDSYWEKLQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCH1</accession>
    <name>A0A452HCH1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fanconi anemia group F protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HCH1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012481"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014303.1">
        <property type="protein sequence ID" value="ENSGAGP00000012481.1"/>
        <property type="gene ID" value="ENSGAGG00000009590.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.490:FF:000001">
        <property type="entry name" value="Fanconi anemia, complementation group F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035428">
        <property type="entry name" value="FANCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038505">
        <property type="entry name" value="FANCF_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14449:SF2">
        <property type="entry name" value="FANCONI ANEMIA GROUP F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14449">
        <property type="entry name" value="FANCONI ANEMIA GROUP F PROTEIN FANCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11107">
        <property type="entry name" value="FANCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="351" mass="39089" checksum="4EEA63F20C11AC18" modified="2019-05-08" version="1">METLLWQVEQLPGLLAVSRSGQVRDWDPPTLDRALQWGRYFQHLHGRFRAQPRLRAALGQRLRRGQHGPPLGFGHLGRCPELLGLALLGNRALPPAACHRLLRSLLLPPSGQATPGPFGLLARRRAAAQLLLPPPSPAQARPEELEQEQSLRTEAQLLLSRLQEDEEEEEEQGPAGVLEHLPRPRVYRVLAALLLEQAGDNAGREGASWAEHREGPRPAAAVLSWLLGEPGRLSVFCRLLPGSLLVALSARYPQLGTPYLHLLTNWGSRLRYDPLSGQWTSSRCPSEDELSWEELRERFSCFMRGPAPLREAALASLKNLKIQDGDFEVCGLSVWTDLLLELGLEVGWRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDQ9</accession>
    <name>A0A452HDQ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SHC-transforming protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Src homology 2 domain-containing-transforming protein C1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HDQ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012939"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014828.1">
        <property type="protein sequence ID" value="ENSGAGP00000012939.1"/>
        <property type="gene ID" value="ENSGAGG00000009926.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007173">
        <property type="term" value="P:epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01209">
        <property type="entry name" value="PTB_Shc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09925">
        <property type="entry name" value="SH2_SHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000036">
        <property type="entry name" value="SHC-transforming protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000005">
        <property type="entry name" value="SHC-transforming protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051235">
        <property type="entry name" value="CEP152/SHC-Transforming"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006019">
        <property type="entry name" value="PID_Shc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035676">
        <property type="entry name" value="SHC_SH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337">
        <property type="entry name" value="SHC TRANSFORMING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337:SF2">
        <property type="entry name" value="SHC-TRANSFORMING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00629">
        <property type="entry name" value="SHCPIDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019539650" description="SHC-transforming protein 1" evidence="9">
        <location>
            <begin position="24"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="10">
        <location>
            <begin position="217"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="11">
        <location>
            <begin position="556"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="433"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="651" mass="70251" checksum="4BD91877DBEFC8BF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HF77</accession>
    <name>A0A452HF77_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Dynein axonemal heavy chain 12</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Consists of at least two heavy chains and a number of intermediate and light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000013458"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015403.1">
        <property type="protein sequence ID" value="ENSGAGP00000013458.1"/>
        <property type="gene ID" value="ENSGAGG00000010280.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000002">
        <property type="entry name" value="Dynein axonemal heavy chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002141">
        <property type="entry name" value="Dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.130:FF:000011">
        <property type="entry name" value="dynein heavy chain 12, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000003">
        <property type="entry name" value="Dynein heavy chain 12, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001112">
        <property type="entry name" value="Dynein heavy chain 12, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000002">
        <property type="entry name" value="Dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000223">
        <property type="entry name" value="Dynein heavy chain 3, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000044">
        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000005">
        <property type="entry name" value="Dynein, axonemal, heavy chain 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000006">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000362">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:005585">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF70">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 2, AXONEMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0969">Cilium</keyword>
    <keyword evidence="13 20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0243">Dynein</keyword>
    <keyword evidence="11" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="22">
        <location>
            <begin position="1273"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="22">
        <location>
            <begin position="1554"/>
            <end position="1708"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="22">
        <location>
            <begin position="1909"/>
            <end position="2057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="640"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="2770"/>
            <end position="2804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00057074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00069442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="3966" mass="454580" checksum="9860655393F4E590" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HG55</accession>
    <name>A0A452HG55_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">sphingomyelin phosphodiesterase</fullName>
            <ecNumber evidence="4">3.1.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-(hexadecanoyl)-sphing-4-enine-1-phosphocholine + H2O = N-hexadecanoylsphing-4-enine + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45644"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72959"/>
            <dbReference type="ChEBI" id="CHEBI:78646"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:45645"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a sphingomyelin + H2O = phosphocholine + an N-acylsphing-4-enine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19253"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17636"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="3.1.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19254"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sphingolipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the neutral sphingomyelinase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.12" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452HG55"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013860"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015868.1">
        <property type="protein sequence ID" value="ENSGAGP00000013860.1"/>
        <property type="gene ID" value="ENSGAGG00000010557.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006684">
        <property type="term" value="P:sphingomyelin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09078">
        <property type="entry name" value="nSMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017766">
        <property type="entry name" value="Sphingomyelinase/PLipase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF9">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="10">
        <location>
            <begin position="206"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="480" mass="53406" checksum="DB0C845BC3B80903" modified="2019-05-08" version="1">FPWKSISSSRCPPPGSRWSRNARAPLPLVPCSMVLRESPFPNRLLSGLYSLGQYLLFPSYWAADCLLDLRETTAERQQRRCCRCCPLRALVTGPLLLLLLILSMPGALLGLLLWLPMQVARRPFAYKHNMLSRHPEVWELPGTGKVFSFVSANVCLLPNGLAKNSNLGQMKQRAARIGQCLTQGEASASFASPRSSQFDGEREAGPREISVPFPPDVDFVCLQEVFDQRAGAHLCQLLSPFYEHIVYNVGAYGLQGCCTLKVLSSGLFLASRYPVLAAQYCCYPNGIREDALSAKGLLSVQVRSCSSCGEPRPVTEPCPLPAHAQIRCDQLTMGLSWVQLFQDTHAQRGDVVAFDVFCGDFNFDNCSSGDELEQTHEIFRQYQDPCRVGPRQDRPWAIGTFRSMLEAEGFTALVQGRLCPWTHTGSPWEGRRIDYILYREHPTTISLTTEVEKFSFITQLAGCSDHVAVGLQLLLNPAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGJ6</accession>
    <name>A0A452HGJ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein YIPF3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">YIP1 family member 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Involved in the maintenance of the Golgi structure. May play a role in hematopoiesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the YIP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGJ6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013983"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016003.1">
        <property type="protein sequence ID" value="ENSGAGP00000013983.1"/>
        <property type="gene ID" value="ENSGAGG00000010648.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051521">
        <property type="entry name" value="tRNA_Mod/Golgi_Maint"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15627">
        <property type="entry name" value="NATURAL KILLER CELL-SPECIFIC ANTIGEN KLIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15627:SF14">
        <property type="entry name" value="PROTEIN YIPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="222"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="380" mass="41272" checksum="A270155EDEBDCFA1" modified="2019-05-08" version="1">MSVSVTSGSRLGFPEPHSEARSGAERPGPVPPQPRGMMSGPGGAAGAGGGARSGAGAEWGGFEDNLQGGGSAVIDMENMDDTSGSSFEDMGEMHQRMKEEEEDVDAEAAAVDEEDGEFLGMKGFKGQLGRQVADQMWQAGKRQASKAFSLYANIDILRPYFDVEPIQVRNRLLESMIPMKMINFPQKIAGELYGPLMLVFTLVAILLHGMKTSDTIIREGTLMGTAIGTCFGYWLGVSSFIYFLAYLCNAQITMVQMLSLLGYGLFGHCITLFVTYNIHFHSLFYIFWLVIGGLSTLRMIAVLVSRTVGHTQRLILCGTLAALHMLFLLYLHFAYHKVVEGILDTLEGPNVPPFQRVARDIPVVASAVLNTTAKAIALTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGL3</accession>
    <name>A0A452HGL3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Runt-related transcription factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGL3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014004"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016026.1">
        <property type="protein sequence ID" value="ENSGAGP00000014004.1"/>
        <property type="gene ID" value="ENSGAGG00000010664.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002062">
        <property type="term" value="P:chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051094">
        <property type="term" value="P:positive regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.720:FF:000001">
        <property type="entry name" value="Runt-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.770.10">
        <property type="entry name" value="Signal Protein Aml-1b, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000040">
        <property type="entry name" value="AML1_Runt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012346">
        <property type="entry name" value="p53/RUNT-type_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013524">
        <property type="entry name" value="Runt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027384">
        <property type="entry name" value="Runx_central_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013711">
        <property type="entry name" value="RunxI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016554">
        <property type="entry name" value="TF_Runt-rel_RUNX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950">
        <property type="entry name" value="RUNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950:SF43">
        <property type="entry name" value="RUNT-RELATED TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00853">
        <property type="entry name" value="Runt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08504">
        <property type="entry name" value="RunxI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009374">
        <property type="entry name" value="TF_Runt-rel_RUNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00967">
        <property type="entry name" value="ONCOGENEAML1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51062">
        <property type="entry name" value="RUNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Runt" evidence="9">
        <location>
            <begin position="23"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="339"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="357"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR009374-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="379" mass="41234" checksum="8A37F7BE2EAD6641" modified="2019-05-08" version="1">MGENSGALSAPAPGSRSRPEVRSVVDVLADHAGELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGDVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPTQVATYHRAIKVTVDGPREPRRHRQKLEDQAKPYTDRYSELERLRQSMRVTPTTPNPRGSLTAPGHFGSQTQTPIQGTSDLSPFSDPRQFDRSFPTLPALTETRFSDPRMHYPGAMSAAFPYTATPSATGISGLSMTSMPTTTRFHHTYLPPPYPGSTQNQSGPFQTNPSPYHLYYGTSSGSYQFSMVAGGERSPTRMLSSCTSASAGNNLMNPNLANQNDGVDADGSHSNSPTAMTTTGRMDESVWRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HH90</accession>
    <name>A0A452HH90_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Transmembrane protein 85</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HH90"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014234"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016292.1">
        <property type="protein sequence ID" value="ENSGAGP00000014234.1"/>
        <property type="gene ID" value="ENSGAGG00000010837.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009445">
        <property type="entry name" value="TMEM85/Emc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19315">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06417">
        <property type="entry name" value="EMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="181" mass="19865" checksum="F56D3ECB1268644A" modified="2019-05-08" version="1">MGTPLVASRGRRFKWAIELGGPGSGGRGRNDRGSGQGESLYPVGYSDKQVPDTSVQETDRILVEKRCWDVALGPLKQIPMNLFIMYMAGNTISIFPTMMVCMMAWRPLQALMAISATFKLLESSSQKFLQGLVYLIGNLLGLALAVYKCQSMGLLPTHASDWLAFIEPPERMEYTGGGLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJE1</accession>
    <name>A0A452HJE1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transmembrane protein 47</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM47 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJE1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015027"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017165.1">
        <property type="protein sequence ID" value="ENSGAGP00000015027.1"/>
        <property type="gene ID" value="ENSGAGG00000011359.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009545">
        <property type="entry name" value="Claudin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015664">
        <property type="entry name" value="P53_induced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14399">
        <property type="entry name" value="P53-INDUCED PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14399:SF12">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06653">
        <property type="entry name" value="Claudin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19014" checksum="9FFEAC6F54B3D86C" modified="2019-05-08" version="1">MGEVRVLRPFKLIALVCLLVALALDVVALLSPAWVTSERGSLSLWEACKRAPDVWHCLSTLRTDWQVATLVLILTAATITLLSFLASLVSFCLGTPRRYYRVVAVLLLVAVVLQVCALILYPIKFIDSSMLKSYHEFNWGYGLGWGSAIFMLGAAILYCLRMDVYQDAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJP9</accession>
    <name>A0A452HJP9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJP9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015137"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017293.1">
        <property type="protein sequence ID" value="ENSGAGP00000015137.1"/>
        <property type="gene ID" value="ENSGAGG00000011429.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF9">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019544318" description="Ig-like domain-containing protein" evidence="12">
        <location>
            <begin position="17"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="176"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="308"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="460" mass="49641" checksum="EB077B97BD670DCC" modified="2019-05-08" version="1">MNLGLILICGMLALKAEVPVELAPGSQLRSVDVVLDCHYVEEAVGEFPGAFAGSFSSDPATLVLRGISIADDGSLDSVTNYEAPGTKTDSTFPIIFEVSASLVPVPYAESLLHADCAGEEVTCEISPYSLHLADEGPSQASWFMGTLKLSSGISIALVLKVSSDSEEDDRMAVLHPRLKVPVSKEGTVLTTVEFQLSSRTPTLRTRFGTSVTLDCSFALASSSPLASLEWRLQHRGSGRRVFHYQTGDMAQAEQSTAHVDVVQLLETGDASLSLHGVGVGDEGTYICLVSTHQHQAQNIIQLQLVEPPRVRLFPELVSREGDGATTLTCEISGYYPLDVSVKWTWEAPDDKDKVPISDSSTYFSSHRQAQDGTYSINSYLSINTATELRPVTYSCHVSHLALEEPITASAQLRVPEQKISKGLVGAFIATFIFFAALVGLLLRTRKTVDPKSEKSLETSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKB3</accession>
    <name>A0A452HKB3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tripartite motif containing 47</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HKB3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015357"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017539.1">
        <property type="protein sequence ID" value="ENSGAGP00000015357.1"/>
        <property type="gene ID" value="ENSGAGG00000011578.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19842">
        <property type="entry name" value="Bbox1_TRIM25-like_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF59">
        <property type="entry name" value="TRIPARTITE MOTIF-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="20"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="170"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="11">
        <location>
            <begin position="401"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="625" mass="70091" checksum="5C329E5A8F88AA4B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HL78</accession>
    <name>A0A452HL78_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Epidermal growth factor receptor kinase substrate 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Stereocilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Synapse</location>
            <location evidence="16">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the EPS8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HL78"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015678"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017907.1">
        <property type="protein sequence ID" value="ENSGAGP00000015678.1"/>
        <property type="gene ID" value="ENSGAGG00000011825.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032420">
        <property type="term" value="C:stereocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900029">
        <property type="term" value="P:positive regulation of ruffle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01210">
        <property type="entry name" value="PTB_EPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09540">
        <property type="entry name" value="SAM_EPS8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11764">
        <property type="entry name" value="SH3_Eps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000023">
        <property type="entry name" value="Epidermal growth factor receptor kinase substrate 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000174">
        <property type="entry name" value="epidermal growth factor receptor kinase substrate 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000071">
        <property type="entry name" value="Epidermal growth factor receptor kinase substrate 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039801">
        <property type="entry name" value="EPS8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055093">
        <property type="entry name" value="EPS8_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033928">
        <property type="entry name" value="EPS8_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035462">
        <property type="entry name" value="Eps8_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013625">
        <property type="entry name" value="PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041418">
        <property type="entry name" value="SAM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12287:SF21">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR KINASE SUBSTRATE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12287">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR KINASE SUBSTRATE EPS8-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22975">
        <property type="entry name" value="EPS8_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08416">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18016">
        <property type="entry name" value="SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="10" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="SH3" evidence="20">
        <location>
            <begin position="516"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="601"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="604"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="638"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="659"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00068628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="810" mass="90259" checksum="EDBB767BD9524166" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HM04</accession>
    <name>A0A452HM04_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the BAR homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HM04"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015960"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018235.1">
        <property type="protein sequence ID" value="ENSGAGP00000015960.1"/>
        <property type="gene ID" value="ENSGAGG00000012009.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000103">
        <property type="entry name" value="Homeobox protein BarH-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050848">
        <property type="entry name" value="Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333:SF15">
        <property type="entry name" value="BARX HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333">
        <property type="entry name" value="HOMEO BOX HB9 LIKE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="130"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="132"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="84"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="193"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="224"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="273" mass="30661" checksum="E7B5509FA1658A73" modified="2019-05-08" version="1">MHCHGELRLASPGQLKAARRRYKTFMIDEILSKETCDYFEKLSLYSVYPSLIVRPKPLHSCTGSPSLRAYPLISVITRQPSVVPHLGPAAPSSRALSPHSSLGATSAETQASETHASSESEGEQLTPRLKKPRRSRTIFTELQLMGLEKKFQKQKYLSTPDRLDLAQSLGLTQLQVKTWYQNRRMKWKKMVLKGGQEAPTKPKGRPKKNSIPSSEEIEAEEKMNSQVQRQILETSQAPEEPELTEEKPEVSLETGKSPEHTLESSPEETTPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HM50</accession>
    <name>A0A452HM50_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein UXT</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Ubiquitously expressed transcript protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UXT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HM50"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016016"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018298.1">
        <property type="protein sequence ID" value="ENSGAGP00000016016.1"/>
        <property type="gene ID" value="ENSGAGG00000012043.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23158">
        <property type="entry name" value="Prefoldin_UXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000007">
        <property type="entry name" value="UXT isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003994">
        <property type="entry name" value="UXT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00293">
        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13345">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13345:SF9">
        <property type="entry name" value="PROTEIN UXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01502">
        <property type="entry name" value="UXTPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00070776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00082448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="151" mass="17533" checksum="535BCDB22A89FD03" modified="2019-05-08" version="1">MGPAEKVPRYEAFVSDVLQRDLWRVQQQREEVYEKITQHMWLKTVIERLQEADGRAVQTQVDLGYNFFVNADVPDSSRIFVALGYGFFAELTLTEALRFVERKTKLLIELSESLTKDSAKIKANIRMVLEGLRELQGFQDLPEDPRRDLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMF6</accession>
    <name>A0A452HMF6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Prokineticin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Endocrine-gland-derived vascular endothelial growth factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AVIT (prokineticin) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HMF6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016126"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018414.1">
        <property type="protein sequence ID" value="ENSGAGP00000016126.1"/>
        <property type="gene ID" value="ENSGAGG00000012110.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001935">
        <property type="term" value="P:endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.80.10:FF:000004">
        <property type="entry name" value="Prokineticin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.80.10">
        <property type="entry name" value="Lipase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009523">
        <property type="entry name" value="Prokineticin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023569">
        <property type="entry name" value="Prokineticin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18821">
        <property type="entry name" value="PROKINETICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18821:SF7">
        <property type="entry name" value="PROKINETICIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06607">
        <property type="entry name" value="Prokineticin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57190">
        <property type="entry name" value="Colipase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0339">Growth factor</keyword>
    <keyword evidence="8" id="KW-0497">Mitogen</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019314616" description="Prokineticin-1" evidence="11">
        <location>
            <begin position="20"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Prokineticin" evidence="12">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="105" mass="11940" checksum="14EF3118A1DDAEE5" modified="2019-05-08" version="1">MKTVIQILCFFLLITFYKCAVITGACERDLQCGAGTCCAISLWLRGLRMCTPLGQEGDECHPFSHKVPFLGKRQHHTCPCLPNFICSRFIDGRFRCSVDFKNIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HPF9</accession>
    <name>A0A452HPF9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aryl hydrocarbon receptor nuclear translocator 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HPF9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016883"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019271.1">
        <property type="protein sequence ID" value="ENSGAGP00000016883.1"/>
        <property type="gene ID" value="ENSGAGG00000012607.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18947">
        <property type="entry name" value="bHLH-PAS_ARNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000003">
        <property type="entry name" value="Aryl hydrocarbon receptor nuclear translocator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000020">
        <property type="entry name" value="Aryl hydrocarbon receptor nuclear translocator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000011">
        <property type="entry name" value="Aryl hydrocarbon receptor nuclear translocator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050933">
        <property type="entry name" value="Circadian_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001067">
        <property type="entry name" value="Nuc_translocat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23042">
        <property type="entry name" value="CIRCADIAN PROTEIN CLOCK/ARNT/BMAL/PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00785">
        <property type="entry name" value="NCTRNSLOCATR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="51"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="122"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="330"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="498"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="566"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="503"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="607"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="639"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="655"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="714" mass="78847" checksum="D528E0F3C05FE49B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQ01</accession>
    <name>A0A452HQ01_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HQ01"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017114"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019533.1">
        <property type="protein sequence ID" value="ENSGAGP00000017114.1"/>
        <property type="gene ID" value="ENSGAGG00000012785.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027870">
        <property type="entry name" value="DUF4543"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48415">
        <property type="entry name" value="GENE 525-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48415:SF1">
        <property type="entry name" value="GENE 525-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15076">
        <property type="entry name" value="DUF4543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019149363" evidence="1">
        <location>
            <begin position="20"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="89" mass="10839" checksum="192985B7477F20D0" modified="2019-05-08" version="1">MKLLVFIFILVILTIFTDTSPILTEKEAKQILRTRREDRPRKAGFPDEPMREHMLYLQRLEQRSEEQFLEHWLNPHCYPHCNRNLVHPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HR08</accession>
    <name>A0A452HR08_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FBXO31 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000017489"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019945.1">
        <property type="protein sequence ID" value="ENSGAGP00000017489.1"/>
        <property type="gene ID" value="ENSGAGG00000012973.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045048">
        <property type="entry name" value="FBXO31/39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706">
        <property type="entry name" value="F-BOX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706:SF130">
        <property type="entry name" value="F-BOX ONLY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12014">
        <property type="entry name" value="Cyclin_D1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="5"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="339"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="488" mass="56046" checksum="94710DE18A723E07" modified="2019-05-08" version="1">PAPAPLSLLELPPELLVQIFGFLPGTDLPSLARVCSTFRRILRXSYGVCENLRKLEITGVSCRDVYAKRINPRVRSGRFMKILPDYEHMEYRDVYTCLLHRYRHILGLWQPDIGPYGGLLNVVVDGFFIIGWMYLPPHDPHVDDPMRFKPLFRIHLMERKSATVECMYGHKGPHNGHIQIVKKDEFSTKCNQTDHHRMSGGRQEEFRTWLREEWGRTLEDIFHEHMQELILMKFIYTSQYDNCLTYRRIYLPPSNADDLLKPGLFKGTYGSHGLEIVMLSFHGKKAKGTKITGDPNIPAGQQTVEIDLAHPIQLPDIENLCNFNELSRIVLEVQEQVRREQQEEEEGQEEEEGHSRQGDSQQVEQPVQRDDAEAEGTEAAGVAATQESALTLQPFVLPVGVISRNKDYPRTCRMCFYGTGLIAGHGFTSPERTPGIFILFDDDRFGFIWLELKSFSLYSRIKVSFQNAEAPSREAFDEMLKNIQSMTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSB9</accession>
    <name>A0A452HSB9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HSB9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017911"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020432.1">
        <property type="protein sequence ID" value="ENSGAGP00000017911.1"/>
        <property type="gene ID" value="ENSGAGG00000013284.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02962">
        <property type="entry name" value="TMX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039101">
        <property type="entry name" value="TMX2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037463">
        <property type="entry name" value="TMX2_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15853">
        <property type="entry name" value="THIOREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15853:SF0">
        <property type="entry name" value="THIOREDOXIN-RELATED TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="141"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="301" mass="33927" checksum="00433C9F0C3EEA4A" modified="2019-05-08" version="1">MAVLAPLLALFYSVPGLCRWLARPYYPLSALLSAAFLLVRKLPPLCQGLPTQREDGNPCDFDWREVEILMFLSAIVMMKNRRSITVDQHLGNIFMFSKVANVILFFRLDVRMGLLYLTLCIVFLMTCKPPLYLGPEYIKYFSDKTIDEELERDKRLTWVVEFFANWSNECQSFAPIYADLSLKYNCTGLNFGKVDVGRYPDVSTRYKVSTSPLTKQLPTLILFQGGKEVMRRPQIDKKGRAVSWTFSEVGCIGAPCRVRGMCNPRPFLGCCRSCVASALPFQSGSSLSIGALAPLPQPWAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSY7</accession>
    <name>A0A452HSY7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tyrosine-protein phosphatase</fullName>
            <ecNumber evidence="11">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A452HSY7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018201"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020746.1">
        <property type="protein sequence ID" value="ENSGAGP00000018201.1"/>
        <property type="gene ID" value="ENSGAGG00000013483.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000020762.1">
        <property type="protein sequence ID" value="ENSGAGP00000018216.1"/>
        <property type="gene ID" value="ENSGAGG00000013483.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13189">
        <property type="entry name" value="FERM_C_PTPN4_PTPN3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17194">
        <property type="entry name" value="FERM_F1_PTPN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06706">
        <property type="entry name" value="PDZ_PTPN3-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000104">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000023">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000003">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041783">
        <property type="entry name" value="PTPN3/4_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012151">
        <property type="entry name" value="Tyr_Pase_non-rcpt_typ-3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706:SF7">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000927">
        <property type="entry name" value="Tyr-Ptase_nr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 11" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="17">
        <location>
            <begin position="29"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="520"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="15">
        <location>
            <begin position="658"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="16">
        <location>
            <begin position="831"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="381"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="455"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="12">
        <location>
            <position position="855"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="823"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="855"/>
            <end position="861"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="899"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000927-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000927-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="929" mass="106328" checksum="A9A49204B612524D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWZ5</accession>
    <name>A0A452HWZ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">E3 ubiquitin-protein ligase TRIM39</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">RING-type E3 ubiquitin transferase TRIM39</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Tripartite motif-containing protein 39</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">E3 ubiquitin-protein ligase. May facilitate apoptosis by inhibiting APC/C-Cdh1-mediated poly-ubiquitination and subsequent proteasome-mediated degradation of the pro-apoptotic protein MOAP1. Regulates the G1/S transition of the cell cycle and DNA damage-induced G2 arrest by stabilizing CDKN1A/p21. Positively regulates CDKN1A/p21 stability by competing with DTL for CDKN1A/p21 binding, therefore disrupting DCX(DTL) E3 ubiquitin ligase complex-mediated CDKN1A/p21 ubiquitination and degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452HWZ5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019699"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022440.1">
        <property type="protein sequence ID" value="ENSGAGP00000019699.1"/>
        <property type="gene ID" value="ENSGAGG00000014528.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19780">
        <property type="entry name" value="Bbox2_TRIM39-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16594">
        <property type="entry name" value="RING-HC_TRIM7-like_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000171">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="15 23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="24">
        <location>
            <begin position="31"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="25">
        <location>
            <begin position="104"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="26">
        <location>
            <begin position="289"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="196"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00056591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00073070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00075969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00076812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="467" mass="53499" checksum="5EB6600B56144501" modified="2019-05-08" version="1">MPHSHTAIPPCLAGTMATTNPLENLQVEASCSVCLEYLKDPVIIDCGHNFCRVCITRWWEELNRDFPCPVCRKTFRHRTLKPNRQLGNMVEIAKQLQATKRKVRDESLCGKHNEVLNLFCKEDQEAVCLVCEISHDHRSHTVMPLDDASLEYKEKLQQCLEPLERKLQDIAHCKSQEEKKPGELKRKVESRRQLIVSEFEELHQFLEEEQQVLLRRLEEEEKEILQKLKDNVAQLSDQRLSLNQLITEIEEKCLQSGIEMLKVRSPEGCETVQTMELASVPIELEKNFCSFPRQYFVLRKIIKRLIGDVTLDPETAHPNLVLSEDRKSVKFVDTRLRDLPDTPRRFTIYPCVLATEGFTSGRHYWEVEVGDKTHWALGVCKDSVSRKGELMALPETGYWRVRLGLGSGFCCSPTCPDYDERRGFYRPPGDPACLWTGRAGAGPDMDYRGTHPSPQTGLGHLVGCALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXQ6</accession>
    <name>A0A452HXQ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Serine/threonine-protein kinase PLK</fullName>
            <ecNumber evidence="16">2.7.11.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Polo-like kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.21" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A452HXQ6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019975"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022758.1">
        <property type="protein sequence ID" value="ENSGAGP00000019975.1"/>
        <property type="gene ID" value="ENSGAGG00000014715.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048167">
        <property type="term" value="P:regulation of synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13117">
        <property type="entry name" value="POLO_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14188">
        <property type="entry name" value="STKc_PLK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001498">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000001">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000091">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.30">
        <property type="entry name" value="POLO box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042825">
        <property type="entry name" value="PLK2_STKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033695">
        <property type="entry name" value="POLO_box_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036947">
        <property type="entry name" value="POLO_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF44">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PLK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00659">
        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82615">
        <property type="entry name" value="Polo-box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="67"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="19">
        <location>
            <begin position="486"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="19">
        <location>
            <begin position="584"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="388"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="668" mass="76113" checksum="0E3ED4D8C0F9B5E1" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYM8</accession>
    <name>A0A452HYM8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable U3 small nucleolar RNA-associated protein 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UTP11 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYM8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020325"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023161.1">
        <property type="protein sequence ID" value="ENSGAGP00000020325.1"/>
        <property type="gene ID" value="ENSGAGG00000014985.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007144">
        <property type="entry name" value="SSU_processome_Utp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03998">
        <property type="entry name" value="Utp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015952">
        <property type="entry name" value="U3snoRNP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="300" mass="34581" checksum="9173E97628EF1B1D" modified="2019-05-08" version="1">MTGRDPALGLGGGACSAAPPSVGSARAQLELPVPERGGQQGGRAGSAMAATFRKAAKAGQREHWERGQPRFRRQLGLLEKKRDYKRRADDYHKKENALKALHKKALEKNPDEFYFKMTRVQLQDGVHVIKQPKEEVTQEQLKVMRTQDLKYVEMKRVAEAKKIERMKSELHLLDAQGMQSNKHVFFFDTKKEVQEFDVATHLNTSPELVDRVYNRPTIETLQKETVKGATHPAHLKKLAQQRKHQYSLLKQRIEREKMMFVIAQKIQARKDLLDKTSKVKVKKETVNSPAVYKFKFQRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0F1</accession>
    <name>A0A452I0F1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I0F1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020935"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023845.1">
        <property type="protein sequence ID" value="ENSGAGP00000020935.1"/>
        <property type="gene ID" value="ENSGAGG00000015390.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052207">
        <property type="entry name" value="Max-like/E-box_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX ZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741:SF14">
        <property type="entry name" value="CARBOHYDRATE-RESPONSIVE ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="475"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="342"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="436"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="526"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="381"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="449"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="679" mass="75122" checksum="8286C6D51DED2622" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0F7</accession>
    <name>A0A452I0F7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">eIF5-mimic protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Basic leucine zipper and W2 domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I0F7"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023911.1">
        <property type="protein sequence ID" value="ENSGAGP00000020991.1"/>
        <property type="gene ID" value="ENSGAGG00000015424.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051245">
        <property type="entry name" value="eIF5-mimic_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057397">
        <property type="entry name" value="HEAT_5MP1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14208">
        <property type="entry name" value="BASIC LEUCINE ZIPPER AND W2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14208:SF7">
        <property type="entry name" value="EIF5-MIMIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25504">
        <property type="entry name" value="HEAT_5MP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="W2" evidence="5">
        <location>
            <begin position="28"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="178"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00039665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00042302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="198" mass="22574" checksum="97E85D0D3BA0E7F4" modified="2019-05-08" version="1">MFTGILLANGTLPAAILTSLFTDNIVKEGIASFAVKLFKSWMAEKDANSVTSSLRKASLDKRLLELFPANRQNVEHFAKYFMDAGLKELSDFLRVQLSLGTRKELQKELQERLSQECPIKEVVLYVKEEMKRNDLPEPAVIGLLEEAILKWYKEAHLAKGKSVFLHQMKKFVEWLQNAEEESESEGEENEDGSTKHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1Z8</accession>
    <name>A0A452I1Z8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Switch-associated protein 70</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I1Z8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021514"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024502.1">
        <property type="protein sequence ID" value="ENSGAGP00000021514.1"/>
        <property type="gene ID" value="ENSGAGG00000015797.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13273">
        <property type="entry name" value="PH_SWAP-70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000175">
        <property type="entry name" value="switch-associated protein 70 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057836">
        <property type="entry name" value="EF-hand_SWAP70_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057837">
        <property type="entry name" value="PH_SWAP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14383">
        <property type="entry name" value="SWAP-70 RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14383:SF6">
        <property type="entry name" value="SWITCH-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25530">
        <property type="entry name" value="EF-hand_SWAP70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="17">
        <location>
            <begin position="245"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="344"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="621" mass="71985" checksum="E84CA0405470004E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2X6</accession>
    <name>A0A452I2X6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal protein L23/L25 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I2X6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021833"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024872.1">
        <property type="protein sequence ID" value="ENSGAGP00000021833.1"/>
        <property type="gene ID" value="ENSGAGG00000016028.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044391">
        <property type="term" value="C:ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000532">
        <property type="entry name" value="50S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="8042" checksum="29F49B431A1226CA" modified="2019-05-08" version="1">MKKIEDKNTLVFIVDVKANNHQIKQAQMWLYNIDVAKVNTLIRPDGEKKVYVRLAPDYDALDVADEIGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I325</accession>
    <name>A0A452I325_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Meiosis regulator and mRNA stability factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Limkain-b1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Meiosis arrest female protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Essential regulator of oogenesis required for female meiotic progression to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Probably acts via some RNA metabolic process, equivalent to the piRNA system in males, which mediates the repression of transposable elements during meiosis by forming complexes composed of RNAs and governs the methylation and subsequent repression of transposons. Also required to protect from DNA double-strand breaks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with LIMK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000021917"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024968.1">
        <property type="protein sequence ID" value="ENSGAGP00000021917.1"/>
        <property type="gene ID" value="ENSGAGG00000016091.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905762">
        <property type="term" value="F:CCR4-NOT complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048477">
        <property type="term" value="P:oogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09978">
        <property type="entry name" value="LOTUS_2_Limkain_b1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09979">
        <property type="entry name" value="LOTUS_3_Limkain_b1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09980">
        <property type="entry name" value="LOTUS_4_Limkain_b1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09981">
        <property type="entry name" value="LOTUS_5_Limkain_b1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09982">
        <property type="entry name" value="LOTUS_6_Limkain_b1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09983">
        <property type="entry name" value="LOTUS_7_Limkain_b1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09984">
        <property type="entry name" value="LOTUS_8_Limkain_b1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10910">
        <property type="entry name" value="PIN_limkain_b1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12255">
        <property type="entry name" value="RRM1_LKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12256">
        <property type="entry name" value="RRM2_LKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.610:FF:000001">
        <property type="entry name" value="Meiosis regulator and mRNA stability factor 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000008">
        <property type="entry name" value="Meiosis regulator and mRNA stability factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.610">
        <property type="entry name" value="LOTUS domain-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041966">
        <property type="entry name" value="LOTUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024768">
        <property type="entry name" value="Marf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045602">
        <property type="entry name" value="MARF1_LOTUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034189">
        <property type="entry name" value="MARF1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034191">
        <property type="entry name" value="MARF1_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025605">
        <property type="entry name" value="OST-HTH/LOTUS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14379">
        <property type="entry name" value="LIMKAIN B LKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14379:SF3">
        <property type="entry name" value="MEIOSIS REGULATOR AND MRNA STABILITY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19687">
        <property type="entry name" value="MARF1_LOTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12872">
        <property type="entry name" value="OST-HTH"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11608">
        <property type="entry name" value="RRM_MARF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51644">
        <property type="entry name" value="HTH_OST"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0896">Oogenesis</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="509"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="794"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="17">
        <location>
            <begin position="1102"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="17">
        <location>
            <begin position="1178"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="17">
        <location>
            <begin position="1262"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="17">
        <location>
            <begin position="1338"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="17">
        <location>
            <begin position="1414"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="17">
        <location>
            <begin position="1489"/>
            <end position="1563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="597"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1661"/>
            <end position="1680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1696" mass="189508" checksum="A45B2B3EDCBBAF89" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3A7</accession>
    <name>A0A452I3A7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3A7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022007"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025072.1">
        <property type="protein sequence ID" value="ENSGAGP00000022007.1"/>
        <property type="gene ID" value="ENSGAGG00000016142.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04516">
        <property type="entry name" value="TBP_eukaryotes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000001">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000002">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033710">
        <property type="entry name" value="TBP_eukaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="36"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="57"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="301" mass="33063" checksum="09E090F918D005FA" modified="2019-05-08" version="1">MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPVQSSNSLSILEEQQRQQQQQAAQQSTSQQATQGTSSQTPQLFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSGIVPQLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKGFRKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3H3</accession>
    <name>A0A452I3H3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">mRNA-capping enzyme</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="17">mRNA 5'-triphosphate monophosphatase</fullName>
                <ecNumber evidence="17">3.6.1.74</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="17">mRNA 5'-phosphatase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="17">mRNA guanylyltransferase</fullName>
                <ecNumber evidence="17">2.7.7.50</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="17">GTP--RNA guanylyltransferase</fullName>
                <shortName evidence="17">GTase</shortName>
            </alternativeName>
        </domain>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14 17">Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphate monophosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the GMP moiety of GTP to the 5'-diphosphate terminus of RNA via a covalent enzyme-GMP reaction intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:67012"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.7.7.50"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:67013"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67004"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
            <dbReference type="EC" id="3.6.1.74"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:67005"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15 17">In the C-terminal section; belongs to the eukaryotic GTase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16 17">In the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.74" evidence="17"/>
    <dbReference type="EC" id="2.7.7.50" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A452I3H3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022041"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025110.1">
        <property type="protein sequence ID" value="ENSGAGP00000022041.1"/>
        <property type="gene ID" value="ENSGAGG00000016170.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000025117.1">
        <property type="protein sequence ID" value="ENSGAGP00000022046.1"/>
        <property type="gene ID" value="ENSGAGG00000016170.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000025123.1">
        <property type="protein sequence ID" value="ENSGAGP00000022049.1"/>
        <property type="gene ID" value="ENSGAGG00000016170.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140818">
        <property type="term" value="F:mRNA 5'-triphosphate monophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004484">
        <property type="term" value="F:mRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004651">
        <property type="term" value="F:polynucleotide 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07895">
        <property type="entry name" value="Adenylation_mRNA_capping"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17664">
        <property type="entry name" value="Mce1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000111">
        <property type="entry name" value="mRNA-capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.430:FF:000001">
        <property type="entry name" value="mRNA-capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000040">
        <property type="entry name" value="mRNA-capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000040">
        <property type="entry name" value="mRNA-capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017074">
        <property type="entry name" value="mRNA_cap_enz_bifunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001339">
        <property type="entry name" value="mRNA_cap_enzyme_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013846">
        <property type="entry name" value="mRNA_cap_enzyme_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF17">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03919">
        <property type="entry name" value="mRNA_cap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01331">
        <property type="entry name" value="mRNA_cap_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036958">
        <property type="entry name" value="mRNA_capping_HCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 20" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 17" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="2 17" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 17 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="22">
        <location>
            <begin position="25"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="23">
        <location>
            <begin position="104"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="180"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="577"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="193"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="18">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="active site" description="N6-GMP-lysine intermediate" evidence="19">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="348"/>
            <end position="350"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="463"/>
            <end position="465"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="533"/>
            <end position="538"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR036958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR036958-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR036958-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR036958-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="602" mass="69239" checksum="5A885D0D4EB2F7B8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4L0</accession>
    <name>A0A452I4L0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I4L0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022501"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025632.1">
        <property type="protein sequence ID" value="ENSGAGP00000022501.1"/>
        <property type="gene ID" value="ENSGAGG00000016502.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03593">
        <property type="entry name" value="CLECT_NK_receptors_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050828">
        <property type="entry name" value="C-type_lectin/matrix_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033992">
        <property type="entry name" value="NKR-like_CTLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710:SF35">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 2 MEMBER D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="5">
        <location>
            <begin position="90"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="217" mass="23860" checksum="5A3F18E4C1614E6B" modified="2019-05-08" version="1">MAEESAEATSDTSGGVTGAMLRGHGPVEERGRDVDFSLKCIKGKRIPIIVTVVITALTITIIALAAMKTPPCPGCPPHVTAACSDGWVGYQGKCYYFSETEGNWNNSQSNCSSLNSSLTSIDSLPELDFMLRYKGVPYHWIGLRREQGEGQPWKWTNGTIFNNLFPVRGEGQCTYLDEYGVSSSRCYSERHFICKRPDKCSRRKPSAAGGDTIFKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5G8</accession>
    <name>A0A452I5G8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">THD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tumor necrosis factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I5G8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022825"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026006.1">
        <property type="protein sequence ID" value="ENSGAGP00000022825.1"/>
        <property type="gene ID" value="ENSGAGG00000016732.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030890">
        <property type="term" value="P:positive regulation of B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00184">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006052">
        <property type="entry name" value="TNF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051748">
        <property type="entry name" value="TNF_Ligand_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15151">
        <property type="entry name" value="PROTEIN EIGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15151:SF2">
        <property type="entry name" value="TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 13B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00229">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50049">
        <property type="entry name" value="THD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="THD" evidence="8">
        <location>
            <begin position="160"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="300" mass="33629" checksum="8FF6AD3ADE427192" modified="2019-05-08" version="1">PRKRKWLRRWFRSRKRRWLKLEVPAWARASAAGGRAPAAEENEARGPRRARFQPGSGPGSAARLTVCLYHVLTLKAELATLRSELIYRIQARTPLPQAQDESKQGTVFSSSLQLTAAAARQEIRLPASEAGEADEKRGWSTGRSRGRRSALPTEEIMLQACLQLIADSKSNIQQKDDSSIVPWLLSFKRGTALEEQGNKILVKEAGYFFIYGQVLYTDTTFAMGHLIQRKKAHVFGDDLSLVTLFRCIQNMPHSYPNNSCYTAGIAKLEEGDELQLTIPRRRAKISLDGDGTFFGAVRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I797</accession>
    <name>A0A452I797_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I797"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023500"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026785.1">
        <property type="protein sequence ID" value="ENSGAGP00000023500.1"/>
        <property type="gene ID" value="ENSGAGG00000017230.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000026786.1">
        <property type="protein sequence ID" value="ENSGAGP00000023501.1"/>
        <property type="gene ID" value="ENSGAGG00000017230.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031523">
        <property type="term" value="C:Myb complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028226">
        <property type="entry name" value="LIN37"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31336">
        <property type="entry name" value="LIN37 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31336:SF3">
        <property type="entry name" value="PROTEIN LIN-37 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15306">
        <property type="entry name" value="LIN37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="282" mass="31506" checksum="3A815CC5FB49D761" modified="2019-05-08" version="1">MGGLQTAGPLAALGRCRVAAAGVGGREDSRPQVTMLPTKVEIEKQDQETTNARSRLDAVLQCLLEKSDVDREQMDEDAGKTSSDPLSKDSSPSAAGKRPSARFSHHRRKKRKETDDGLTESGQQRQNTYIIKLFDRSVDLAQFAESTPLYPICRAWMRNSPAVRERERSPSPPLPTLPEDEEGAEGLNGKSQDVYKLPPPCAGLENAGGESVSARIPSPLPPEDGESPSDMILDARPSMSSLIYKNMERWKKIRQRWKEASHRNQLRYTESMKILKEMYERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9Q1</accession>
    <name>A0A452I9Q1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">TGF-beta family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Dimeric, linked by one or more disulfide bonds. Inhibin A is a dimer of alpha and beta-A. Inhibin B is a dimer of alpha and beta-B. Activin A is a homodimer of beta-A. Activin B is a homodimer of beta-B. Activin AB is a dimer of beta-A and beta-B.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimeric or heterodimeric through association with alpha and beta subunits, linked by one or more disulfide bonds. Inhibins are heterodimers of one alpha and one beta subunit. Activins are homo- or heterodimers of beta subunits only.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I9Q1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024414"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027789.1">
        <property type="protein sequence ID" value="ENSGAGP00000024414.1"/>
        <property type="gene ID" value="ENSGAGG00000017837.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19406">
        <property type="entry name" value="TGF_beta_INHBC_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000005">
        <property type="entry name" value="Inhibin beta A chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001318">
        <property type="entry name" value="Inhibin_betaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF130">
        <property type="entry name" value="INHIBIN BETA C CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00672">
        <property type="entry name" value="INHIBINBC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0339">Growth factor</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019334473" description="TGF-beta family profile domain-containing protein" evidence="13">
        <location>
            <begin position="22"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="14">
        <location>
            <begin position="236"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="356" mass="39207" checksum="D4DFAC38FA3EBCED" modified="2019-05-08" version="1">MATGQALLLLILSGVAKPGAGRCPSCEMAALDPEVQKTVLIELAKQSILSKLHLKERPNVTQPVFRAGLLTALGRLRVGRTNQSITLEMPNQSSKTDEQEYEILSFAEPGPAAPNRMRLHFRFTQDAGRSVQVLHADMYVFLAAPGGPACRVTAKLLLSEPGNANPTLVGRRQLEVKQGGWAAVEVRTAVQSFFSKGGQRLTAELELEGSPEPALLLSSSESHRPFMVAKTRARPRHRLHRRAVECNSNSRTCCRKQFFVDFKEIGWEDWIIQPEGYHMNYCTGPCPTHVASMPGLASSFHTAILNLIKANDVHAAFSSCCIPTRRRPLSLLYYDQDSNVIKTDIPDMIVEACGCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9Y6</accession>
    <name>A0A452I9Y6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452I9Y6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024337"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027701.1">
        <property type="protein sequence ID" value="ENSGAGP00000024337.1"/>
        <property type="gene ID" value="ENSGAGG00000017782.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14040">
        <property type="entry name" value="STKc_TLK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000037">
        <property type="entry name" value="Serine/threonine-protein kinase tousled-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TOUSLED-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="457"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="346"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="408"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="70"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="89"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="107"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="353"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="486"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="767" mass="86617" checksum="85648294DFB71284" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB19</accession>
    <name>A0A452IB19_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Myosin heavy chain 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IB19"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024884"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028326.1">
        <property type="protein sequence ID" value="ENSGAGP00000024884.1"/>
        <property type="gene ID" value="ENSGAGG00000018187.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF857">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50084">
        <property type="entry name" value="Myosin S1 fragment, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="11">
        <location>
            <begin position="27"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="10">
        <location>
            <begin position="81"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20852" checksum="3985EC71FB85B44A" modified="2019-05-08" version="1">MAQKDVDKYLYVDKNLINNPLAQADWAAKKLVWVPSEKNGFEAASLKEEVGDEAIVELAENGKKVRINKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKERYYSGLIYTYSGLFCVVINPYKNLPIYSEEIVEMYKGKKRHEMPPHIYAITDTAYRSMMQGECQLWDSDFLSEILKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBU8</accession>
    <name>A0A452IBU8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tetratricopeptide repeat protein 30</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Required for polyglutamylation of axonemal tubulin. Plays a role in anterograde intraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium to the site of their incorporation at the tip.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell projection</location>
            <location evidence="1 9">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TTC30/dfy-1/fleer family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IBU8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025244"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028740.1">
        <property type="protein sequence ID" value="ENSGAGP00000025244.1"/>
        <property type="gene ID" value="ENSGAGG00000018464.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120170">
        <property type="term" value="F:intraciliary transport particle B binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000226">
        <property type="entry name" value="Tetratricopeptide repeat protein 30A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000211">
        <property type="entry name" value="tetratricopeptide repeat protein 30B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039941">
        <property type="entry name" value="TT30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20931">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20931:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7 9" id="KW-0969">Cilium</keyword>
    <keyword evidence="4 9" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0802">TPR repeat</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="668" mass="75754" checksum="E536CACCF195169D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ID23</accession>
    <name>A0A452ID23_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18 22">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="18 22">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor as well as interleukin (IL)-10 receptor. Kinase partner for the type I interferon receptor IFNAR2. In response to interferon-binding to IFNAR1-IFNAR2 heterodimer, phosphorylates and activates its binding partner IFNAR2, creating docking sites for STAT proteins. Directly phosphorylates STAT proteins but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 18 22">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="18 22"/>
    <dbReference type="AlphaFoldDB" id="A0A452ID23"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025702"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029242.1">
        <property type="protein sequence ID" value="ENSGAGP00000025702.1"/>
        <property type="gene ID" value="ENSGAGG00000018762.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005131">
        <property type="term" value="F:growth hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007259">
        <property type="term" value="P:cell surface receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098761">
        <property type="term" value="P:cellular response to interleukin-7"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060397">
        <property type="term" value="P:growth hormone receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05077">
        <property type="entry name" value="PTK_Jak1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000110">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000135">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000293">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000055">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000114">
        <property type="entry name" value="Tyrosine-protein kinase JAK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041155">
        <property type="entry name" value="FERM_F1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041046">
        <property type="entry name" value="FERM_F2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051286">
        <property type="entry name" value="JAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041381">
        <property type="entry name" value="JAK1-3/TYK2_PHL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016251">
        <property type="entry name" value="Tyr_kinase_non-rcpt_Jak/Tyk2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020776">
        <property type="entry name" value="Tyr_kinase_non-rcpt_Jak1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45807">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE HOPSCOTCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45807:SF5">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE JAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18379">
        <property type="entry name" value="FERM_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18377">
        <property type="entry name" value="FERM_F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17887">
        <property type="entry name" value="Jak1_Phl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21990">
        <property type="entry name" value="SH2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000636">
        <property type="entry name" value="TyrPK_Jak"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01823">
        <property type="entry name" value="JANUSKINASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01824">
        <property type="entry name" value="JANUSKINASE1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 18 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 18 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 18 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="4 18 22" id="KW-0808">Transferase</keyword>
    <keyword evidence="15 18 22" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="FERM" evidence="25">
        <location>
            <begin position="34"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="607"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="900"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="124"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="349"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="1028"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="906"/>
            <end position="914"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20 21">
        <location>
            <position position="933"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00054436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000636-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000636-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1181" mass="136599" checksum="A074132A7592DCC5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ID74</accession>
    <name>A0A452ID74_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sodium/calcium exchanger 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Na(+)/Ca(2+)-exchange protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Solute carrier family 8 member 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Mediates the exchange of one Ca(2+) ion against three to four Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to Ca(2+) transport during excitation-contraction coupling in muscle. In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A1 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline. Required for normal embryonic heart development and the onset of heart contractions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>Ca(2+)(in) + 3 Na(+)(out) = Ca(2+)(out) + 3 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:69955"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC8 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ID74"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025718"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029258.1">
        <property type="protein sequence ID" value="ENSGAGP00000025718.1"/>
        <property type="gene ID" value="ENSGAGG00000018770.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000001">
        <property type="entry name" value="sodium/calcium exchanger 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2030:FF:000001">
        <property type="entry name" value="sodium/calcium exchanger 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051171">
        <property type="entry name" value="CaCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003644">
        <property type="entry name" value="Calx_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004836">
        <property type="entry name" value="Na_Ca_Ex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032452">
        <property type="entry name" value="Na_Ca_Ex_C-exten"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002987">
        <property type="entry name" value="NaCa_exhngr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00845">
        <property type="entry name" value="caca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878">
        <property type="entry name" value="SODIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878:SF6">
        <property type="entry name" value="SODIUM_CALCIUM EXCHANGER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03160">
        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16494">
        <property type="entry name" value="Na_Ca_ex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01260">
        <property type="entry name" value="NACAEXCHNGR1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00237">
        <property type="entry name" value="Calx_beta"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="14" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="18 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 26" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0915">Sodium</keyword>
    <keyword evidence="20" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="9 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019566218" description="Sodium/calcium exchanger 1" evidence="26">
        <location>
            <begin position="33"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="27">
        <location>
            <begin position="301"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00032110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00033667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00045279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="599" mass="66883" checksum="A38130DA4AFDC5C3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDR2</accession>
    <name>A0A452IDR2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025837"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029392.1">
        <property type="protein sequence ID" value="ENSGAGP00000025837.1"/>
        <property type="gene ID" value="ENSGAGG00000018863.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000029401.1">
        <property type="protein sequence ID" value="ENSGAGP00000025845.1"/>
        <property type="gene ID" value="ENSGAGG00000018863.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16481">
        <property type="entry name" value="RING-H2_TTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056872">
        <property type="entry name" value="TTC3/DZIP3-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056870">
        <property type="entry name" value="TTC3/DZIP3/RBM44-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043866">
        <property type="entry name" value="TTC3/DZIP3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056871">
        <property type="entry name" value="WH_TTC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17550">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17550:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24525">
        <property type="entry name" value="TTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24905">
        <property type="entry name" value="TTC3_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19179">
        <property type="entry name" value="TTC3_DZIP3_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24812">
        <property type="entry name" value="WHD_TTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="201"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="1922"/>
            <end position="1962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="348"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="740"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1873"/>
            <end position="1917"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1407"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1524"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="742"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1882"/>
            <end position="1894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1903"/>
            <end position="1915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1981" mass="226544" checksum="7EA185FC95CBE3FE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IEE5</accession>
    <name>A0A452IEE5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HnRNP M nuclear localisation signal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IEE5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026201"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029797.1">
        <property type="protein sequence ID" value="ENSGAGP00000026201.1"/>
        <property type="gene ID" value="ENSGAGG00000019123.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024666">
        <property type="entry name" value="HnRNP_M_PY-NLS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11532">
        <property type="entry name" value="HnRNP_M_NLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HnRNP M nuclear localisation signal" evidence="2">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="96" mass="10173" checksum="44AF69CAD09B0D25" modified="2019-05-08" version="1">MAAVGAEGSGKMEEAAAAAAANGAGSAGDGPPKSEGERPSQNEKRKEKSVKRGGNRFEPYANPTKRYRAFITNIPFDVKWQSLKDLVKEKGNGCFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFD2</accession>
    <name>A0A452IFD2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="20">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of saturated and unsaturated C16 or C18 fatty acyl-CoA to fatty alcohols. It plays an essential role in the production of ether lipids/plasmalogens which synthesis requires fatty alcohols. In parallel, it is also required for wax monoesters production since fatty alcohols also constitute a substrate for their synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(9Z)-octadecenoyl-CoA + 2 NADPH + 2 H(+) = (9Z)-octadecen-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:36323"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:73504"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:36324"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(9Z,12Z)-octadecadienoyl-CoA + 2 NADPH + 2 H(+) = (9Z,12Z)-octadecadien-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:36363"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:73534"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:36364"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>16-methylheptadecanoyl-CoA + 2 NADPH + 2 H(+) = 16-methylheptadecan-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:81763"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:84911"/>
            <dbReference type="ChEBI" id="CHEBI:231998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:81764"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>18-methylnonadecanoyl-CoA + 2 NADPH + 2 H(+) = 18-methylnonadecan-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:81767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:84914"/>
            <dbReference type="ChEBI" id="CHEBI:231999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:81768"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:52717"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>eicosanoyl-CoA + 2 NADPH + 2 H(+) = eicosan-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:81727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:75627"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:81728"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>hexadecanoyl-CoA + 2 NADPH + 2 H(+) = hexadecan-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:36315"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16125"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:36316"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>octadecanoyl-CoA + 2 NADPH + 2 H(+) = octadecan-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:36319"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32154"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:36320"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A452IFD2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026552"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030186.1">
        <property type="protein sequence ID" value="ENSGAGP00000026552.1"/>
        <property type="gene ID" value="ENSGAGG00000019312.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000123">
        <property type="entry name" value="Fatty acyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF119">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 20" id="KW-0521">NADP</keyword>
    <keyword evidence="7 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="471"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="22">
        <location>
            <begin position="15"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acyl-CoA reductase C-terminal" evidence="21">
        <location>
            <begin position="360"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="518" mass="59826" checksum="D147C1E73A8448F8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGC5</accession>
    <name>A0A452IGC5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myotrophin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the myotrophin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IGC5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026960"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030641.1">
        <property type="protein sequence ID" value="ENSGAGP00000026960.1"/>
        <property type="gene ID" value="ENSGAGG00000019624.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070531">
        <property type="term" value="C:BRCA1-A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031436">
        <property type="term" value="C:BRCA1-BARD1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010613">
        <property type="term" value="P:positive regulation of cardiac muscle hypertrophy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000118">
        <property type="entry name" value="Myotrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="34"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="118" mass="12893" checksum="6097EFDF69F3B7DF" modified="2019-05-08" version="1">MSDKEFMWALKNGDLDEVKDYVAKGEDVNRTLEGGRKPLHYAADCGQLEILEFLLLKGADINAPDKHHITPLLSAVYEGHVTCVKLLLSKGADKTVKGPDGLTAFEATDNQAIKALLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGL4</accession>
    <name>A0A452IGL4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 770</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IGL4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026907"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030583.1">
        <property type="protein sequence ID" value="ENSGAGP00000026907.1"/>
        <property type="gene ID" value="ENSGAGG00000019591.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002212">
        <property type="entry name" value="Zinc finger protein 672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000286">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000904">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000978">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001194">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001196">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001860">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF218">
        <property type="entry name" value="ZINC FINGER PROTEIN 502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="493"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="521"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="658"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="686"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="713" mass="83437" checksum="F0519970E41EE315" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIH5</accession>
    <name>A0A452IIH5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Receptor expression-enhancing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the DP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IIH5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027638"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031398.1">
        <property type="protein sequence ID" value="ENSGAGP00000027638.1"/>
        <property type="gene ID" value="ENSGAGG00000020087.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071782">
        <property type="term" value="C:endoplasmic reticulum tubular network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031849">
        <property type="term" value="F:olfactory receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF33">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="159"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="281" mass="31998" checksum="BBDBEFD80B297975" modified="2019-05-08" version="1">MVSWIISRLVVIIFGTLYPAYYSYKAVKSKDIKEYVKWMMYWIIFALFTTAETVTDLFLCWFPFYYELKIAFVAWLLSPYTKGSSVLYRKFVHPTLSSKEKEIDDCLVQAKDRSYDALVHFGKRGLNVAATAAVMAASKGQGALSERLRSFSMQDLSTIREESSSPVPPPVTVRTSSKQSKPKTSRSASESTGSSVCSCCSVCRILRVVKVDMDDACEKPRETNCETKDLVFSDDEEHDLLDHMVKNRRLKKKEQALEAPPRPLRPRFRKKSTSISTTETM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIW6</accession>
    <name>A0A452IIW6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">COPII-coated vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
            <location evidence="12">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IIW6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027778"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031557.1">
        <property type="protein sequence ID" value="ENSGAGP00000027778.1"/>
        <property type="gene ID" value="ENSGAGG00000020194.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01478">
        <property type="entry name" value="Sec23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11287">
        <property type="entry name" value="Sec23_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.730:FF:000003">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1670:FF:000006">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000003">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000011">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037550">
        <property type="entry name" value="Sec23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF7">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="14">
        <location>
            <begin position="58"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="15">
        <location>
            <begin position="126"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="17">
        <location>
            <begin position="401"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="16">
        <location>
            <begin position="518"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="13">
        <location>
            <begin position="632"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="765" mass="86172" checksum="C7F06020DDB214A8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IK76</accession>
    <name>A0A452IK76_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">FCS-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IK76"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028281"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032136.1">
        <property type="protein sequence ID" value="ENSGAGP00000028281.1"/>
        <property type="gene ID" value="ENSGAGG00000020545.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20120">
        <property type="entry name" value="MBT_MBTD1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20123">
        <property type="entry name" value="MBT_MBTD1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20126">
        <property type="entry name" value="MBT_MBTD1_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000010">
        <property type="entry name" value="MBT domain-containing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000015">
        <property type="entry name" value="MBT domain-containing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000019">
        <property type="entry name" value="MBT domain-containing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000032">
        <property type="entry name" value="MBT domain-containing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.160:FF:000001">
        <property type="entry name" value="MBT domain-containing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004092">
        <property type="entry name" value="Mbt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050548">
        <property type="entry name" value="PcG_chromatin_remod_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012313">
        <property type="entry name" value="Znf_FCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038603">
        <property type="entry name" value="Znf_FCS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF79">
        <property type="entry name" value="MBT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02820">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21319">
        <property type="entry name" value="zf-FCS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00561">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51079">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51024">
        <property type="entry name" value="ZF_FCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FCS-type" evidence="13">
        <location>
            <begin position="68"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="MBT" evidence="11">
        <location>
            <begin position="165"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="MBT" evidence="11">
        <location>
            <begin position="277"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="repeat" description="MBT" evidence="11">
        <location>
            <begin position="375"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="repeat" description="MBT" evidence="11">
        <location>
            <begin position="488"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="582"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="696"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="598"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="665"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="697"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="716" mass="80152" checksum="9833532734608B71" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILQ6</accession>
    <name>A0A452ILQ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILQ6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028840"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032766.1">
        <property type="protein sequence ID" value="ENSGAGP00000028840.1"/>
        <property type="gene ID" value="ENSGAGG00000020895.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF25">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="265" mass="29966" checksum="DE315AFB8C321AAB" modified="2019-05-08" version="1">MASQDPQGQINKIFQDYLPGSPDLPSQSKPIEPTEAWKHVQPPHSLPPGLEYLSQLDQIIIHQQVELLEVILGTETCSKYEIKNHLGQRVYFAMEENGCFDRNFCSPIRSFTIRITDNTGREVITVNRPLRCNSCWFPCYMQELEVQSPPGTIVGYVVQNWDPLLPKFTILNESKEDVLKIIGPYATCSCFGDVDFEVKTLNEMSTIGKISKYWSGFVNDVFTNAANFGIQVPVDLDVKIKAIMIGACFLIVSTQNQREKPSQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMU9</accession>
    <name>A0A452IMU9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000029408"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033394.1">
        <property type="protein sequence ID" value="ENSGAGP00000029408.1"/>
        <property type="gene ID" value="ENSGAGG00000021259.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16866">
        <property type="entry name" value="ARID_ARID2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000253">
        <property type="entry name" value="AT-rich interactive domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052406">
        <property type="entry name" value="Chromatin_Remodeling_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22970">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22970:SF14">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02257">
        <property type="entry name" value="RFX_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ARID" evidence="6">
        <location>
            <begin position="13"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="RFX-type winged-helix" evidence="7">
        <location>
            <begin position="521"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="890"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="958"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1250"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1375"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1442"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1703"/>
            <end position="1729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="900"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="958"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="980"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1013"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1250"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1300"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1375"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1393"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1417"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1442"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1708"/>
            <end position="1728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1835" mass="198510" checksum="B220F76ED8652D31" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQE2</accession>
    <name>A0A452IQE2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IQE2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030120"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034197.1">
        <property type="protein sequence ID" value="ENSGAGP00000030120.1"/>
        <property type="gene ID" value="ENSGAGG00000021729.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF107">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="11">
        <location>
            <begin position="44"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="12">
        <location>
            <begin position="183"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="296" mass="33631" checksum="281E9ABA735AD005" modified="2019-05-08" version="1">PGSWRGLRPSPRPAGSLPSPPPRRILLTVVVIFRILIVAIVGETVYEDEQTMFMCNTLQPGCNQACYDKAFPISHIRYWVFQIILVCTPSLCFITYSVHQSAKQRDRRYSFLYPLLEKDARKARSLNGILVHNPEGSAKEEPDCLEVKELPSTPRRPPKSAKVKRQEGISRFYIIQVVFRNALEIGFLAGQYFLYGFNVPGIFECDRYPCVREVECYVSRPTEKTVFLVFMFAVSGICVLLNLAELNHLGWRKIKTAIRGVQARRKSVCEVRKKDLSALAQVPPLGRTQSSESAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ISB3</accession>
    <name>A0A452ISB3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in intracellular trafficking and contributes to the macromolecular organization of group 1 metabotropic glutamate receptors (mGluRs) at synapses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Postsynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ISB3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030818"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034950.1">
        <property type="protein sequence ID" value="ENSGAGP00000030818.1"/>
        <property type="gene ID" value="ENSGAGG00000022148.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06713">
        <property type="entry name" value="PDZ_tamalin_CYTIP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000157">
        <property type="entry name" value="General receptor for phosphoinositides 1-associated scaffold protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052122">
        <property type="entry name" value="Intracell_Traff_Signaling_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15963">
        <property type="entry name" value="GENERAL RECEPTOR FOR PHOSPHOINOSITIDES 1-ASSOCIATED SCAFFOLD PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15963:SF3">
        <property type="entry name" value="PROTEIN TAMALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="PDZ" evidence="13">
        <location>
            <begin position="20"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="218"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="233"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="300" mass="33142" checksum="13F62717D106359B" modified="2019-05-08" version="1">RRGTGSLRPLLMTLSLPRKMITLEKEAEETFGFEIQTYGLHHQDKKSVEMFTFVCRVHEGSPAQLAGLTPGDTITAVNGLNVEGIRHREIVEIIKASGNALRLETLYGTSIRRAELEARLQYLKQTLYEKWGEYRSLMVQEQRLVHGIVVKDPSIYDTLESVRSCLYSAGLASGSLAFGKMLSGAGSTSSCVSTATEESEDSVYQTCFFDSADSLDGHSGRDGGSAPPVLSQRRPTLTRSASVKCTSSSNGAVGHFWDRPGDQKNCTITPHKSKHTSFRKRLLRFIPGLNRSLEEEESHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITG1</accession>
    <name>A0A452ITG1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ITG1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031333"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035541.1">
        <property type="protein sequence ID" value="ENSGAGP00000031333.1"/>
        <property type="gene ID" value="ENSGAGG00000022518.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000035">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000039">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000041">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001330">
        <property type="entry name" value="Zinc finger protein ZIC 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041643">
        <property type="entry name" value="Znf_ZIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18366">
        <property type="entry name" value="zf_ZIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="335"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="365"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="395"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="33"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="158"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="408"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="411"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="431"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="468" mass="51494" checksum="450FF6D2A905FA60" modified="2019-05-08" version="1">MSVDALGSPVMDPAALSKRNTALRLVDLAGAHHHHHHLHPPQSMTGFPGFAGHPHSVASTHPGEYAAESRLGPNPFRPEHMGHHHHHHHHHHPHHPAALKLSPAPHPHPHHQCHHHHHHHMAGQAEVVSSQTGTFGPAQSTAVPYPVSRTAQAISAGSYPGHHGHHHSEAGNHSLFSGLHEQPPHATPGGHLNGQIRLGLPGEMYARSELFTQVPASRTDPFAASSLHSYGGMNLNVNLAPHHGPGAFFRYMRQPIKQELICKWIELDQTPKKLCSKTFTTMHELVTHVTVEHVGGPEQSNHICFWEECPREGKPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFKCEFEGCDRRFANSSDRKKHSHVHTSDKPYNCKVRGCDKSYTHPSSLRKHMKVHCKSPPPSSGYESSTPSLVSPSSDSGRDPPASCSHAEPVASSQTAANLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITL1</accession>
    <name>A0A452ITL1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Melanocortin 2 receptor accessory protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MRAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ITL1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031383"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035597.1">
        <property type="protein sequence ID" value="ENSGAGP00000031383.1"/>
        <property type="gene ID" value="ENSGAGG00000022553.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031780">
        <property type="term" value="F:corticotropin hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030545">
        <property type="term" value="F:signaling receptor regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070996">
        <property type="term" value="F:type 1 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031781">
        <property type="term" value="F:type 3 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031782">
        <property type="term" value="F:type 4 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031783">
        <property type="term" value="F:type 5 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106070">
        <property type="term" value="P:regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028111">
        <property type="entry name" value="MRAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28675:SF2">
        <property type="entry name" value="MELANOCORTIN-2 RECEPTOR ACCESSORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28675">
        <property type="entry name" value="MELANOCORTIN-2 RECEPTOR ACCESSORY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15183">
        <property type="entry name" value="MRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="16144" checksum="D08D4B6A475E9295" modified="2019-05-08" version="1">MAIKTNSSEYYWSYEYYWDYLDPVPVDERKLKANKYSIVIAFWVGLAAFVVFLFLILLYMSRSGSTPVNYKYHTVLPKQSTFIHMAEPLRRQHNTYKKQHKFLHTPTIYMHKHTQNHPPCTVSLVGQHPFQPFGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITM3</accession>
    <name>A0A452ITM3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NLR family member X1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction. Instead, promotes autophagy by interacting with TUFM and subsequently recruiting the autophagy-related proteins ATG5 and ATG12. Also regulates MAVS-dependent NLRP3 inflammasome activation to attenuate apoptosis. Has no inhibitory function on NF-kappa-B signaling pathway, but enhances NF-kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species. Regulates viral mediated-inflammation and energy metabolism in a sex-dependent manner. In females, prevents uncontrolled inflammation and energy metabolism and thus, may contribute to the sex differences observed in infectious and inflammatory diseases.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homohexamer. Interacts with MAVS. Interacts with TUFM.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ITM3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031236"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035427.1">
        <property type="protein sequence ID" value="ENSGAGP00000031233.1"/>
        <property type="gene ID" value="ENSGAGG00000022453.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000035430.1">
        <property type="protein sequence ID" value="ENSGAGP00000031236.1"/>
        <property type="gene ID" value="ENSGAGG00000022453.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001029">
        <property type="entry name" value="NLR family member X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000149">
        <property type="entry name" value="NLR family member X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048900">
        <property type="entry name" value="NLRX1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21402">
        <property type="entry name" value="NLRX1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="9" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="NACHT" evidence="16">
        <location>
            <begin position="187"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="998" mass="112187" checksum="79A13079A99A132A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITU2</accession>
    <name>A0A452ITU2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CCR4-NOT transcription complex subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CCR4-associated factor 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CNOT1 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000031530"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035757.1">
        <property type="protein sequence ID" value="ENSGAGP00000031530.1"/>
        <property type="gene ID" value="ENSGAGG00000022646.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20710">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.800:FF:000001">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000005">
        <property type="entry name" value="Ccr4-not transcription complex subunit 1 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.790:FF:000001">
        <property type="entry name" value="Ccr4-not transcription complex subunit 1 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.840:FF:000001">
        <property type="entry name" value="Ccr4-not transcription complex subunit 1 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055454">
        <property type="entry name" value="CNOT1-like_NOT1_connector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055104">
        <property type="entry name" value="CNOT1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032194">
        <property type="entry name" value="CNOT1_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22940">
        <property type="entry name" value="CNOT1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16418">
        <property type="entry name" value="CNOT1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23590">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 N-terminal" evidence="19">
        <location>
            <begin position="30"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat" evidence="18">
        <location>
            <begin position="500"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="17">
        <location>
            <begin position="815"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="16">
        <location>
            <begin position="1089"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="15">
        <location>
            <begin position="1388"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1-like NOT1 connector" evidence="20">
        <location>
            <begin position="1611"/>
            <end position="1815"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="14">
        <location>
            <begin position="1999"/>
            <end position="2358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1316"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1316"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1334"/>
            <end position="1346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF23590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2377" mass="267184" checksum="DE2CF6D1D4623679" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUW3</accession>
    <name>A0A452IUW3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgin subfamily A conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IUW3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031859"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036134.1">
        <property type="protein sequence ID" value="ENSGAGP00000031859.1"/>
        <property type="gene ID" value="ENSGAGG00000022877.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000036136.1">
        <property type="protein sequence ID" value="ENSGAGP00000031861.1"/>
        <property type="gene ID" value="ENSGAGG00000022877.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000036139.1">
        <property type="protein sequence ID" value="ENSGAGP00000031864.1"/>
        <property type="gene ID" value="ENSGAGG00000022877.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000036142.1">
        <property type="protein sequence ID" value="ENSGAGP00000031866.1"/>
        <property type="gene ID" value="ENSGAGG00000022877.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000137">
        <property type="term" value="C:Golgi cis cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024858">
        <property type="entry name" value="GOLGA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043937">
        <property type="entry name" value="GOLGA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043976">
        <property type="entry name" value="GOLGA_cons_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10881:SF46">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10881">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19046">
        <property type="entry name" value="GM130_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15070">
        <property type="entry name" value="GOLGA2L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A conserved" evidence="4">
        <location>
            <begin position="410"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="195"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="280"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="494"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="697"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="768"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="900"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1049" mass="119255" checksum="1E64750387B9830B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IVY5</accession>
    <name>A0A452IVY5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IVY5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032184"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036487.1">
        <property type="protein sequence ID" value="ENSGAGP00000032184.1"/>
        <property type="gene ID" value="ENSGAGG00000023038.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000036490.1">
        <property type="protein sequence ID" value="ENSGAGP00000032186.1"/>
        <property type="gene ID" value="ENSGAGG00000023038.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000607">
        <property type="entry name" value="Leukemia inhibitory factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000657">
        <property type="entry name" value="Leukemia inhibitory factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000738">
        <property type="entry name" value="Leukemia inhibitory factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001286">
        <property type="entry name" value="Oncostatin-M-specific receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001289">
        <property type="entry name" value="Oncostatin-M-specific receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048497">
        <property type="entry name" value="LIF-R-like_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040817">
        <property type="entry name" value="LIFR_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052672">
        <property type="entry name" value="Type1_Cytokine_Rcpt_Type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423:SF1">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21177">
        <property type="entry name" value="LIF-R_Ig-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17971">
        <property type="entry name" value="LIFR_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25552">
        <property type="entry name" value="LIFR_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604599" description="Fibronectin type-III domain-containing protein" evidence="11">
        <location>
            <begin position="23"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="750"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="333"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="433"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="634"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1000" mass="113781" checksum="C85AA3B118E80FD9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWF6</accession>
    <name>A0A452IWF6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IWF6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032430"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036768.1">
        <property type="protein sequence ID" value="ENSGAGP00000032430.1"/>
        <property type="gene ID" value="ENSGAGG00000023206.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000103">
        <property type="entry name" value="Si:ch73-109i22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14388:SF9">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14388">
        <property type="entry name" value="T CELL-SPECIFIC ADAPTER PROTEIN TSAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="4">
        <location>
            <begin position="167"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="70"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="261"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="454"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="266"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="560" mass="60743" checksum="D12CCADBCD8A9579" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWG8</accession>
    <name>A0A452IWG8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000032440"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036785.1">
        <property type="protein sequence ID" value="ENSGAGP00000032440.1"/>
        <property type="gene ID" value="ENSGAGG00000023215.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030169">
        <property type="term" value="F:low-density lipoprotein particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005041">
        <property type="term" value="F:low-density lipoprotein particle receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000040">
        <property type="entry name" value="Stabilin 2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000278">
        <property type="entry name" value="Stabilin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000017">
        <property type="entry name" value="Stabilin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000018">
        <property type="entry name" value="Stabilin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000021">
        <property type="entry name" value="Stabilin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056806">
        <property type="entry name" value="EGF_STAB1-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24038">
        <property type="entry name" value="STABILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24038:SF0">
        <property type="entry name" value="STABILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24887">
        <property type="entry name" value="EGF_STAB1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 9" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="166"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="208"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="293"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="11">
        <location>
            <begin position="336"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="11">
        <location>
            <begin position="488"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="708"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="796"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="837"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="880"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="923"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="11">
        <location>
            <begin position="964"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="11">
        <location>
            <begin position="1107"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="1306"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="1351"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="1417"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="1459"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="736"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1334"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1359"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1378"/>
            <end position="1387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1564" mass="170815" checksum="9107BBC118D8A4D3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYN9</accession>
    <name>A0A452IYN9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Recycling endosome membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYN9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033216"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037640.1">
        <property type="protein sequence ID" value="ENSGAGP00000033216.1"/>
        <property type="gene ID" value="ENSGAGG00000023682.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000284">
        <property type="entry name" value="RAB11 family interacting protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2440:FF:000001">
        <property type="entry name" value="RAB11 family interacting protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037245">
        <property type="entry name" value="FIP-RBD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019018">
        <property type="entry name" value="Rab-bd_FIP-RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057316">
        <property type="entry name" value="Rab11-FIP3/4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051977">
        <property type="entry name" value="Rab11-interacting_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726:SF5">
        <property type="entry name" value="RAB11 FAMILY-INTERACTING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726">
        <property type="entry name" value="RAB11-FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25450">
        <property type="entry name" value="Rab11-FIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09457">
        <property type="entry name" value="RBD-FIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144270">
        <property type="entry name" value="Eferin C-derminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51511">
        <property type="entry name" value="FIP_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="14"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="FIP-RBD" evidence="15">
        <location>
            <begin position="570"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="214"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="361"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="535"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="633" mass="72640" checksum="B05964E6E81A6997" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYR9</accession>
    <name>A0A452IYR9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger CCCH domain-containing protein 15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ZC3H15/TMA46 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYR9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033227"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037653.1">
        <property type="protein sequence ID" value="ENSGAGP00000033227.1"/>
        <property type="gene ID" value="ENSGAGG00000023689.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000050">
        <property type="entry name" value="AGAP008634-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="175"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="175"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="361"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="381"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="412"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="428" mass="48680" checksum="C1F06D335F0F2013" modified="2019-05-08" version="1">MPPKKPPPAAGPNKKADQKKKEKIIEDKTFGLKNKKGAKQQKFIKAVTHQVKFGQQNPRQAAQAEGDKKLKKDDKKKELQELNELFKPVVAAQKISKGADPKSVVCAFFKQGQCTKGDKCKFSHDLTLERKCEKRSVYIDARDEDLEKDTMDTWDEKKLEEVVNKKHGEAEKKKPKTQIVCKYFLDAIENNKYGWFWVCPGGGDNCMYRHALPPGFILKKEKKKEEKEDEVSLEDLIERERAALGPNITKITLESFLAWKKRKRQEKIDKAEQDMERRKADFKAGKALVISGREVFEFRPELVDADDEEADDTNYIQGSSEDEVEDLVRINDIDLNLYIPKDVDETGITVASLERFSAYTSVEKDDNKLSEASGGGIENGEQSDLEENEAEGEQENGVIDAVPVDENLFTGEDLDELEEELNTLDLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZT9</accession>
    <name>A0A452IZT9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZT9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033623"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038088.1">
        <property type="protein sequence ID" value="ENSGAGP00000033623.1"/>
        <property type="gene ID" value="ENSGAGG00000023941.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15394">
        <property type="entry name" value="7tmA_PrRP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000291">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001402">
        <property type="entry name" value="Prolrel_pep_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235:SF14">
        <property type="entry name" value="PROLACTIN RELEASING HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01018">
        <property type="entry name" value="PRPRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="58"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="350" mass="40301" checksum="3D98A53BB7E2C849" modified="2019-05-08" version="1">MEPIGNWDRLPTNDSINEIILKNSSNSSSQFTGVQLIQSFKPLIIPCYTLVVLIGIFGNYLLLYVICRTKKMHNVTNFFIGNLAFSDMLMCATCVPFTLAYAFNPQGWIFGKFLCYFVFLMQPMTVYVSVFTLTAIAVDRYYATVHPLKKRISVTTCVYVVGGIWLLSCALVAPAIAHTYHVEFQKEGFAICEEFWMEEEKERLAYAYSTLIITYILPLSAVSLSYICITVKLKNRVVPGHPTQSQTEFDRVRKRKVFRLIVLVVAAFGICWLPIHVFNIIRDIDINLINKHYFLLIQLLCHWFAMSSSCCNPFLYAWLHDRFRSELKKMFTFKQKIIPTNNCIAVSVML</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1M2</accession>
    <name>A0A452J1M2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U3 small nucleolar RNA-associated protein 25 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Digestive organ expansion factor homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">UTP25 small subunit processor component</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the ribosomal small subunit processome for the biogenesis of ribosomes, functions in pre-ribosomal RNA (pre-rRNA) processing. Essential for embryonic development in part through the regulation of p53 pathway. Controls the expansion growth of digestive organs and liver. Also involved in the sympathetic neuronal development. Mediates, with CAPN3, the proteasome-independent degradation of p53/TP53.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with CAPN3; the interaction is required for CAPN3 translocation to the nucleolus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UTP25 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J1M2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034287"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038825.1">
        <property type="protein sequence ID" value="ENSGAGP00000034287.1"/>
        <property type="gene ID" value="ENSGAGG00000024363.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000962">
        <property type="entry name" value="digestive organ expansion factor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010678">
        <property type="entry name" value="UTP25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053939">
        <property type="entry name" value="UTP25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053940">
        <property type="entry name" value="UTP25_NTPase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933">
        <property type="entry name" value="ORF PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06862">
        <property type="entry name" value="Utp25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22916">
        <property type="entry name" value="UTP25_NTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UTP25 NTP hydrolase-like" evidence="13">
        <location>
            <begin position="305"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="UTP25 C-terminal" evidence="12">
        <location>
            <begin position="582"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="84"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="772" mass="89152" checksum="78AAD8208DA8C1F4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J2E9</accession>
    <name>A0A452J2E9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J2E9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034573"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039145.1">
        <property type="protein sequence ID" value="ENSGAGP00000034573.1"/>
        <property type="gene ID" value="ENSGAGG00000024576.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042327">
        <property type="term" value="P:positive regulation of phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18385">
        <property type="entry name" value="BTB_POZ_BTBD10_GMRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000017">
        <property type="entry name" value="BTB/POZ domain-containing protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039886">
        <property type="entry name" value="BTBD10/KCTD20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039885">
        <property type="entry name" value="BTBD10/KCTD20_BTB/POZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21637">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21637:SF5">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16017">
        <property type="entry name" value="BTB_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="174"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="43"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="482" mass="54208" checksum="41001254FBE931BA" modified="2019-05-08" version="1">MPKDAELASKAAFLEGADFFCTLISKLFPCFCISSLVYTRGSTPSRVAKGVDNTRMSLHGASGGHERSRDRRRSSDRSRDSSHERSESQLTPCIRNVTSPTRQHHGDREKDHSSSRPSSPRPQKTSPNGSNSSVGNSSRNSSQSSSDGSCKTAGEMVFVYENAKEGARSVRTSERVTLIVDNTRFVVDPSIFTAQPNTMLGRMFGSGREHNFTRPNEKGEYEVAEGIGSTVFRAILDYYKTGIIRCPDGISIPELREACDYLCISFEYSTIKCRDLSALMHELSNDGARRQFEFYLEEMILPLMVASAQSGERECHIVVLTDDDVVDWDEEYPPQMGEEYSQIIYSTKLYRFFKYIENRDVAKSVLKERGLKKIRLGIEGYPTYKEKVKKRPGGRPEVIYNYVQRPFIRMSWEKEEGKSRHVDFQCVKSKSITNLAAAAADIPQDQLVVMHPTPQVDELDILPIHPPPSNNDVDPEGLNPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J308</accession>
    <name>A0A452J308_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Alpha/beta-tubulin-N-acetyltransferase 9</fullName>
            <ecNumber evidence="6">2.3.1.308</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">N-acetyltransferase that mediates the acetylation of the N-terminal residues of alpha- and beta-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N-terminal L-methionyl-[tubulin] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-[tubulin] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69607"/>
            <dbReference type="Rhea" id="RHEA-COMP:17729"/>
            <dbReference type="Rhea" id="RHEA-COMP:17730"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="ChEBI" id="CHEBI:133414"/>
            <dbReference type="EC" id="2.3.1.308"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family. GNAT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.308" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452J308"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034700"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039281.1">
        <property type="protein sequence ID" value="ENSGAGP00000034700.1"/>
        <property type="gene ID" value="ENSGAGG00000024651.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120519">
        <property type="term" value="F:tubulin N-terminal-methionine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000040">
        <property type="entry name" value="N-acetyltransferase 9 (putative)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039135">
        <property type="entry name" value="NAT9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13256:SF16">
        <property type="entry name" value="ALPHA_BETA-TUBULIN-N-ACETYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13256">
        <property type="entry name" value="N-ACETYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="8">
        <location>
            <begin position="14"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00051141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00058998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="209" mass="24141" checksum="A0A43EB54B81EDE7" modified="2019-05-08" version="1">MKINQNTVLQGKKVTLVPYTSAHVSRYHEWMKSEELQRLTASEPLSLEQEYEMQRSWQEDADKCTFIVLDTERWSGHTGTEEDCMVGDVNLFLTDMEDPTVGEIEVMIAEPSCRGQGFGKETTLIMMFYGVMKLGITKFEAKVGQENEASISMFKKLHFKEVAVNRVFQEVTLKLDVNEQQRQWLLEQTNHVEEKNYNVLNLQTCASET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J373</accession>
    <name>A0A452J373_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J373"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034755"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039341.1">
        <property type="protein sequence ID" value="ENSGAGP00000034755.1"/>
        <property type="gene ID" value="ENSGAGG00000024687.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051171">
        <property type="entry name" value="CaCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004836">
        <property type="entry name" value="Na_Ca_Ex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878">
        <property type="entry name" value="SODIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878:SF7">
        <property type="entry name" value="SODIUM_CALCIUM EXCHANGER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01259">
        <property type="entry name" value="NACAEXCHNGR"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="7">
        <location>
            <begin position="111"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="191" mass="21446" checksum="E5EAFF4B6B1C2B71" modified="2019-05-08" version="1">IEEEEAKRIAEMGKPVLGEHPKLEVIIEESYEFKSTVDKLIKKTNLALVVGTHSWRDQFMEAITVSAAGDEDEDESGEERLPSCFDYVMHFLTVFWKVLFACVPPTEYCNGWACFVVSIMIIGMLTAVIGDLASHFGCTIGLKDSVTAVVFVAFGTSVPGEESVILRLFSHLQRSHFLYFIVFFFLSSIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J439</accession>
    <name>A0A452J439_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J439"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035214"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039874.1">
        <property type="protein sequence ID" value="ENSGAGP00000035214.1"/>
        <property type="gene ID" value="ENSGAGG00000025032.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000009">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF96">
        <property type="entry name" value="CADHERIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019461512" description="Cadherin domain-containing protein" evidence="7">
        <location>
            <begin position="26"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="8">
        <location>
            <begin position="80"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="8">
        <location>
            <begin position="161"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="253" mass="28370" checksum="5A998EEC96D8363F" modified="2019-05-08" version="1">MFTRNCLSLLLWVLFDGGLLTPLHPQLQQTLEAQPRENVVTIPGRRSSAQRVKRGWVWNQFFVLEEYMGSEPQYVGKLHSDLDKGAGTVKYTLSGDGAGTVFTIDETTGDIHAIRSLDREEKPFYTLRAQAVDIDTRKPLEPESEFIIKVQDINDNEPKFLDGPYVASVPEMSPVGAYVLQVKATDADDPTYGNSARVVYSILQGQPYFSIDPKTGKCFISNNIIILLSNIYILIKQMGGSIIGVYRNKYLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5G2</accession>
    <name>A0A452J5G2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxypeptidase Q</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Plasma glutamate carboxypeptidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Carboxypeptidase that may play an important role in the hydrolysis of circulating peptides. Catalyzes the hydrolysis of dipeptides with unsubstituted terminals into amino acids. May play a role in the liberation of thyroxine hormone from its thyroglobulin (Tg) precursor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homodimer. The monomeric form is inactive while the homodimer is active.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J5G2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035721"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040448.1">
        <property type="protein sequence ID" value="ENSGAGP00000035721.1"/>
        <property type="gene ID" value="ENSGAGG00000025362.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000040451.1">
        <property type="protein sequence ID" value="ENSGAGP00000035724.1"/>
        <property type="gene ID" value="ENSGAGG00000025362.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006590">
        <property type="term" value="P:thyroid hormone generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03883">
        <property type="entry name" value="M28_Pgcp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000036">
        <property type="entry name" value="Carboxypeptidase Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000112">
        <property type="entry name" value="Carboxypeptidase Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000009">
        <property type="entry name" value="Carboxypeptidase Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039866">
        <property type="entry name" value="CPQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12053:SF3">
        <property type="entry name" value="CARBOXYPEPTIDASE Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12053">
        <property type="entry name" value="PROTEASE FAMILY M28 PLASMA GLUTAMATE CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="11 23" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="17" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604622" description="Carboxypeptidase Q" evidence="23">
        <location>
            <begin position="19"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="24">
        <location>
            <begin position="268"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00025534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00025833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00033328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="486" mass="53928" checksum="BB730F32045EA946" modified="2019-05-08" version="1">MKFLFLIAFIHLLPQCTGKPLRWDDCHQHTFKEIKREIAGYSDIAKAIINLAVYGNAQNRSYERLARFVDTIGPRLSGSKNLEMAIKYMFSVLQDDGLENVHLEPVKVPHWERGEESAMMLAPRNHSIVILGLGSSVATPPEGITAEVLVVTSFDELQKRAQDAKGKIVVYNQPFISYKETVKYRLLGAAEAGKVGAVASLIRSIASFSVNSPHTGWQQYQSDVPKIPTACISVEDAEMMARMSSRGTKIVVTLKMGAKTYPDTGSFNTVAEIVGSKYPEQVVLVSGHLDSWDVGQGAMDDGGGAFISWEALSLIKDLGLRPKRTLRLVLWTAEEPGGVGAEQYYQLHKDNISNFDIVMESDSGTFMPMGLAFTGSDKVRAILKEIMQLLQPINVTNIYENGSETDTEYWVRDGVPGASLRDDISKYFWFHHSQGDTMTVQDPSQMNLCAAVWTVVSYVIADMEEMLPSCTVMDRAGIHDIYVYCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J6T0</accession>
    <name>A0A452J6T0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aspartate dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the L-aspartate dehydrogenase family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000036212"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040993.1">
        <property type="protein sequence ID" value="ENSGAGP00000036212.1"/>
        <property type="gene ID" value="ENSGAGG00000025651.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033735">
        <property type="term" value="F:aspartate dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002811">
        <property type="entry name" value="Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31873:SF6">
        <property type="entry name" value="ASPARTATE DEHYDROGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31873">
        <property type="entry name" value="L-ASPARTATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01958">
        <property type="entry name" value="Asp_DH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aspartate/homoserine dehydrogenase NAD-binding" evidence="4">
        <location>
            <begin position="49"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate dehydrogenase" evidence="3">
        <location>
            <begin position="199"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="309" mass="33724" checksum="C2687521F26A39CF" modified="2019-05-08" version="1">MLPVWRGQLGLDVETPPPQSLHRRCGALAFWRWLHVSFPDTLPAACQYLVQRLQDEGPRHGLELAFVWNRDVGKLQGKVPVSLQLQDLGWFPECEVDLVVEVAHPCVVRDHGASFLSGADFMXGSPTALADQATEMRLREAARRGGHTLYVPRGALWGGEDIQRMDDRGMLQGLKVTMIKHPDSFRLEGVLRERLGAVRAVLYEGPVRGLCPLAPNNVNTMAAACMAAPSLGFDGVQGCLIADPGLPDWHVLEVEVTGPSGERRDQAFTVTTSRRNPASPGAVTGTATFPSFWSSLLACQGHRGHVVLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GF84</accession>
    <name>A0A452GF84_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GF84"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000196"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000225.1">
        <property type="protein sequence ID" value="ENSGAGP00000000196.1"/>
        <property type="gene ID" value="ENSGAGG00000000174.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026510">
        <property type="entry name" value="PEX11C_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20990">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20990:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 11C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="238" mass="26225" checksum="D5D69BEEF1DAFD50" modified="2019-05-08" version="1">MAAAADPVSSLVSVLESYRGRDRVIRTLCYGCQLAGGVLARKSPAESEMGRRLLAVSAQLSHCRTVLRLFDDLAMLAYSRQYGLGAKEEDTIVRWLSVLNNLADQLYYPCEHVAWAADADVIRTNSHKWWTLSTAFWGLSLVLGIVRSLRILFQLRRKLRNRTGESLEQSQKEIDAQVKSEVLSILSSMADLSNAVHWLPPGFLWAGQFPPWLVGLMGTISSLIGVYQTSVGAGSGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GG53</accession>
    <name>A0A452GG53_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GG53"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000552"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000621.1">
        <property type="protein sequence ID" value="ENSGAGP00000000552.1"/>
        <property type="gene ID" value="ENSGAGG00000000461.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="164" mass="18152" checksum="FCA013E7A5028ABC" modified="2019-05-08" version="1">PCRLNPSPPSSSIPLQSAPRPSQGIPLRPLHPAFPKYPTARPPPSPEDPPTPPSRAPSLPSLETSNYQIQIGSLKHVLPKKRKTLAYQFSVGGHLSTWPAYASGQAILQSRKPCCLPEGRSKKQASLTIAKPRYLEELECYLRKELQSLDLTKENTQELKLQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GH86</accession>
    <name>A0A452GH86_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein JTB</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Required for normal cytokinesis during mitosis. Plays a role in the regulation of cell proliferation. May be a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Increases AURKB activity. Inhibits apoptosis induced by TGFB1. Overexpression induces swelling of mitochondria and reduces mitochondrial membrane potential.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with AURKA, AURKB, BIRC5 and INCENP. May be a component of the CPC at least composed of BIRC5/survivin, CDCA8/borealin, INCENP and AURKB/Aurora-B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the JTB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GH86"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001070.1">
        <property type="protein sequence ID" value="ENSGAGP00000000946.1"/>
        <property type="gene ID" value="ENSGAGG00000000752.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.220:FF:000001">
        <property type="entry name" value="Jumping translocation breakpoint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008657">
        <property type="entry name" value="JTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13041">
        <property type="entry name" value="JTB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13041:SF3">
        <property type="entry name" value="PROTEIN JTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05439">
        <property type="entry name" value="JTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="158" mass="17107" checksum="E4624202A5455D05" modified="2019-05-08" version="1">MSGLSEDQGAAPPKLLACPGGACVGGLPPQTAHLSWGVRLDRALNLSPLFSVSPLVATPCWQAEDFVVVQDCAPCTIFQAKTMPECSTTGFIEQVSCAVSKKEEYKSCRSVVTEAHSFWKFEGAMICVAVVFALLVMCRQRVLDRKALEKVLKQIESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHJ5</accession>
    <name>A0A452GHJ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GHJ5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001083"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001218.1">
        <property type="protein sequence ID" value="ENSGAGP00000001083.1"/>
        <property type="gene ID" value="ENSGAGG00000000874.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="4">
        <location>
            <begin position="295"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="432"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="361"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="389"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="54132" checksum="7CFCC52B4E5898AB" modified="2019-05-08" version="1">MYAKRRKCDLLELCKQRGLRPGRLTKDQLIAQLEQGDRMNERSPVSEGSSRADASQAPVSVPAGSGQPADEGFPRPPLPRRRGRAGRSPVYTEGTVTPPASRGASRRSSPPSRGSSRQRSASVERTRLEYERELRREELELKRRELEEKAKQREHEENQRKHEENQRKHEENQRQREREEKEKQRKHELELARLASSEAPAAVSEGGPKPTKSFDKHLLPRRKEGEDIDTFLTAFENACELHRVDPADRIAVLTPLLDSTAVEVYSRLKGAEAGDYELFKQALLREFGLTPEMYRKKFRSQRKTREVTYLQLVNRAQGYARKWTAGAQTKEDLLDLFILEHLYEQCPSDLRLWLMDQKPENPQHAGQLADQFVDSRAGDGREESRRSRPASTQRESHHGTSQRGPMENPPKRGTSSGRSLRPTQGDPRDMGCYRCGQRGHIRAQCPKLRDRPSRPNPQRVDWVKTQSEEGLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GI06</accession>
    <name>A0A452GI06_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GI06"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001269"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001424.1">
        <property type="protein sequence ID" value="ENSGAGP00000001269.1"/>
        <property type="gene ID" value="ENSGAGG00000001018.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18668">
        <property type="entry name" value="CD1_tandem_CHD5-9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18663">
        <property type="entry name" value="CD2_tandem_CHD5-9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 8 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051493">
        <property type="entry name" value="CHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46850">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46850:SF1">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="3">
        <location>
            <begin position="65"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="164"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="253" mass="30666" checksum="869940551FCADD33" modified="2019-05-08" version="1">MTMEEEEFFVKYKNYSYLHCEWATEQQLLKDKRIQQKIKRFKLRQAQRGHFFADMEEEPFNPDYVEVDRVLEVSFCEDKDTGEPVIYYLVKWCSLPYEDSTWELKEDVDQAKIEEFEQLQASRPDSRRLDRPPPNSWKKIEQSREYKNGNHLREYQLEGLNWLLFNWYNRRNCILADEMGLGKTIQSITFLYEILLTGIRGPFLIIAPLSTITNWEREFRTWTDLNVVVYHGSMVSRQMIQQYEMYFRDSQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKR9</accession>
    <name>A0A452GKR9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DECD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452GKR9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002196"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002505.1">
        <property type="protein sequence ID" value="ENSGAGP00000002196.1"/>
        <property type="gene ID" value="ENSGAGG00000001300.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000111">
        <property type="entry name" value="DEAD-box ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="83"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="228"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="395" mass="44718" checksum="3663C1D74F965148" modified="2019-05-08" version="1">PCGRAWGSWESPFGGLGGGTPWKGLGKLGEPIWGAGTQLVTDCGGGTGERPIEVAWGGQGAAGEVGQGRGGRCTNAPVPQVSVLVMCHTRELAFQISKEYERFSKYMPSVKVAVFFGGLSIKKDEEVLKKNCPHIVVGTPGRILALARNKSLNLKHIKHFILDECDKMLEQLDMRRDVQEIFRMTPHEKQVMMFSATLSKEIRPVCRKFMQDPMEIFVDDETKLTLHGLQQYYVKLKDNEKNRKLFDLLDVLEFNQVVIFVKSVQRCIALAQLLVEQNFPAIAIHRGMPQEERLSRYQQFKDFQRRILVATNLFGRGMDIERVNIAFNYDMPEDSDTYLHRVARAGRFGTKGLAITFVSDENDAKILNDVQDRFEVNISELPDEIDISSYIEQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNH7</accession>
    <name>A0A452GNH7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">PR domain zinc finger protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">PR domain-containing protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">May function as a transcription factor involved in cell differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GNH7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003416"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003939.1">
        <property type="protein sequence ID" value="ENSGAGP00000003416.1"/>
        <property type="gene ID" value="ENSGAGG00000002763.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990226">
        <property type="term" value="F:histone methyltransferase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19189">
        <property type="entry name" value="PR-SET_PRDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000022">
        <property type="entry name" value="PR domain zinc finger protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000436">
        <property type="entry name" value="PR domain zinc finger protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000723">
        <property type="entry name" value="PR domain zinc finger protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000840">
        <property type="entry name" value="PR domain zinc finger protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000922">
        <property type="entry name" value="PR domain zinc finger protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017124">
        <property type="entry name" value="PRDM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044404">
        <property type="entry name" value="PRDM4_PR/SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041493">
        <property type="entry name" value="PRDM4_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF2">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18445">
        <property type="entry name" value="Zn_ribbon_PRDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037161">
        <property type="entry name" value="PRDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019098357" description="PR domain zinc finger protein 4" evidence="17">
        <location>
            <begin position="20"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="19">
        <location>
            <begin position="453"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="631"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="659"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="687"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="715"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="743"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="771"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="839" mass="93072" checksum="D2572BE4FBF0BD91" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQN6</accession>
    <name>A0A452GQN6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQN6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004243"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004981.1">
        <property type="protein sequence ID" value="ENSGAGP00000004243.1"/>
        <property type="gene ID" value="ENSGAGG00000003523.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15221">
        <property type="entry name" value="7tmA_OR52B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000006">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF32">
        <property type="entry name" value="OLFACTORY RECEPTOR 52B6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="95"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="270"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="44"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="321" mass="36059" checksum="E093EF5ADDCDCB7E" modified="2019-05-08" version="1">MMPAYNHTFFAPVTYILTGIPGTEESHVWISIPFCLMYVATLFGNCLLLFIILTERSLHEPMYLFLSMLAAADLLLSTTAVPTMLAVFWFGAGEISFAACLTQMFFIHVSFLAESAIILAMAFDRYVAICDPLRYTMILTKSVIGKLGLAIVTRSFCFNFPVIFLVKQLKFCRTNLLPHIYCEYMAIVRLACDDITVSIWYGLATAILVIGLDAVLIAVSYGLILRAVFQLPSNDARLKAIRTCSSHLCVILMFYTPVFFLIFARRFGHIIPGYILILLANLCVLIPPMLNPIVYGLKTKEILKRVINVFHRWCTGSSLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GS66</accession>
    <name>A0A452GS66_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GS66"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004804"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005623.1">
        <property type="protein sequence ID" value="ENSGAGP00000004804.1"/>
        <property type="gene ID" value="ENSGAGG00000003946.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF156">
        <property type="entry name" value="KRAB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="8"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="111" mass="11643" checksum="B63544B2F5FD46A6" modified="2019-05-08" version="1">AAASPGGCGAGDVAVYFTQGQGALLDPTQRALYRDVMQENYETVTSLGKSVKGWITETLLGAATRCAKTPPVPVFPASSGLQQPVLLSQTLPSAPTQTQGLNYLPQNCRFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSR6</accession>
    <name>A0A452GSR6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GSR6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005020"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005859.1">
        <property type="protein sequence ID" value="ENSGAGP00000005020.1"/>
        <property type="gene ID" value="ENSGAGG00000004097.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001851">
        <property type="term" value="F:complement component C3b binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045957">
        <property type="term" value="P:negative regulation of complement activation, alternative pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032703">
        <property type="term" value="P:negative regulation of interleukin-2 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043031">
        <property type="term" value="P:negative regulation of macrophage activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039939">
        <property type="entry name" value="VSIG4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15466">
        <property type="entry name" value="V-SET AND IMMUNOGLOBULIN DOMAIN CONTAINING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15466:SF2">
        <property type="entry name" value="V-SET AND IMMUNOGLOBULIN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019566204" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="35"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="143"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="346" mass="38751" checksum="52A41F39A3568481" modified="2019-05-08" version="1">MEKFMWLVALVNSFIFCNAILELTGTHDTKGTWKSSITLPCVYGPSQDFVQQTVIWTLVRDQSPATVFRRDNSGDHIMLSRYRGRVSVPKYRPGDVSLEIEKLEMMDSGHYTCKVTWRAQNNSLLTKERTTTVKVVKVSVTKPIIRAGNLGFTVPKGARTSLTCSANGSPPITYRWFKGEPGGNTVLVSNHAVLMFDSLQTSDTGKYYCEAENKASSQVKQQSDAVQLTVRDLTKPAATVPSSENKANTMANDIFIVVCRVTPGSFLPLMLDFCFSQNTRNDRRRQTCSGINGKCKYEEGKPTVENNFMTQPVKENEYETISIKENIECKILVNAMESEYEVGDVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GT43</accession>
    <name>A0A452GT43_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GT43"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005178"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006037.1">
        <property type="protein sequence ID" value="ENSGAGP00000005178.1"/>
        <property type="gene ID" value="ENSGAGG00000004210.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016504">
        <property type="term" value="F:peptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF24">
        <property type="entry name" value="PROCOLLAGEN C-ENDOPEPTIDASE ENHANCER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="34"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="201" mass="21706" checksum="5777822C9A8375CB" modified="2019-05-08" version="1">GAPAELGGQGLRVANGGHRVTVTLGVSPRPVFLCGGEHVGESGYIASEGFPNHYPPGKTCTWTITVPEGQVVILSFRVFDLEPDPGCRYDSLEIFNGHSATAQRLGRFCGTFRPGALLSTSNHMMLRMVTDEGTGGRGFLVWFSSGLPHVNGRPRGRQKWGANHIDSRLAWEPWHYWDGEYLAACPLPPPAMAPPRRAGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTL0</accession>
    <name>A0A452GTL0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LRRNT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GTL0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005384"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006269.1">
        <property type="protein sequence ID" value="ENSGAGP00000005384.1"/>
        <property type="gene ID" value="ENSGAGG00000004369.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000006272.1">
        <property type="protein sequence ID" value="ENSGAGP00000005387.1"/>
        <property type="gene ID" value="ENSGAGG00000004369.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF20">
        <property type="entry name" value="PODOCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604470" description="LRRNT domain-containing protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="6">
        <location>
            <begin position="46"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="582" mass="64913" checksum="AAAFCD909A59588B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GV01</accession>
    <name>A0A452GV01_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GV01"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005807"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006773.1">
        <property type="protein sequence ID" value="ENSGAGP00000005807.1"/>
        <property type="gene ID" value="ENSGAGG00000004721.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14982">
        <property type="entry name" value="7tmA_purinoceptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047160">
        <property type="entry name" value="GP183-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237:SF37">
        <property type="entry name" value="COAGULATION FACTOR II (THROMBIN) RECEPTOR-LIKE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="21"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="343" mass="39849" checksum="6E843FF72BA853E4" modified="2019-05-08" version="1">ADVTRQLEFSPQDRERLEGHSNATIVDCYVEADFQYHLFTIIYSIVFVLGLLENVLALYLLTCKVKHTSHSYIYLINLAVNDWIFGDMACRITGTLYYINIYVSIAFFTCICMDRYVAVLHPFAYIRIKVTHYMIVVTVFWFIAASITIPLIVGGPLHNRGTGNKTACFENFSLSSWTHRMMPYNVCALLFGFVIPFAVILISYPLIAKRIAHTRRSIHKKAALRTIYLILLICILCFLPYHLTHLLHFLMRVQLIQSCPFTNLIYKMRRVTLALVSFNCCLNPILYYFTASSKPWRLSLKFRSKTKLYTICDRKFNDYPYKSKPAARPPTQQLNEMWCQSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GW39</accession>
    <name>A0A452GW39_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphodiesterase</fullName>
            <ecNumber evidence="10">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.4.53"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452GW39"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006280"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007299.1">
        <property type="protein sequence ID" value="ENSGAGP00000006280.1"/>
        <property type="gene ID" value="ENSGAGG00000004053.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00762">
        <property type="reaction ID" value="UER00747"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006198">
        <property type="term" value="P:cAMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000001">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040844">
        <property type="entry name" value="PDE4_UCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18100">
        <property type="entry name" value="PDE4_UCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019339626" description="Phosphodiesterase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="PDEase" evidence="13">
        <location>
            <begin position="167"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="490"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="520"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="532"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="243"/>
            <end position="247"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="575" mass="65316" checksum="E03D05A6FC1FDD1E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWB1</accession>
    <name>A0A452GWB1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GWB1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006365"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007405.1">
        <property type="protein sequence ID" value="ENSGAGP00000006365.1"/>
        <property type="gene ID" value="ENSGAGG00000005135.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042613">
        <property type="term" value="C:MHC class II protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002504">
        <property type="term" value="P:antigen processing and presentation of peptide or polysaccharide antigen via MHC class II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05766">
        <property type="entry name" value="IgC1_MHC_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000116">
        <property type="entry name" value="HLA class II histocompatibility antigen, DRB1-1 beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.320.10:FF:000001">
        <property type="entry name" value="HLA class II histocompatibility antigen, DRB1-1 beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.320.10">
        <property type="entry name" value="Class II Histocompatibility Antigen, M Beta Chain, Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014745">
        <property type="entry name" value="MHC_II_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000353">
        <property type="entry name" value="MHC_II_b_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF99">
        <property type="entry name" value="HLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DRB1 BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00969">
        <property type="entry name" value="MHC_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00921">
        <property type="entry name" value="MHC_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0491">MHC II</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019554128" description="Ig-like domain-containing protein" evidence="12">
        <location>
            <begin position="32"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="128"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="357"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="389" mass="41838" checksum="79C4059AE1DD1DD7" modified="2019-05-08" version="1">MGAGRILGAGSRWAGALLVTLVVLRTHLAHCMEPPRGFVYQANSECYFTNGTERVRFLHRYIYNGRQDVHFDSDLGVHVADTELGRPDAEYWNSQPEILADERAAADRFCRHNYGVAAPFTVARRVRPKVKVSPTKSGSKPHPDLLVCSVTGFYPGGIEIKWLKNGQEQTAGVVSTELLQNGDWTFQILVMLEMSPQRGDVYTCQVEHISLQGPIAVHWEAQSDSARSKMLTGVGGFVLGLIFLAPGLLIYLKNKKGRPVPQPAGNFNPPPPLSPPPPTLSTGCLGHPWGGCFFPSAHDTCPAQPLGSCAMGRWPHGGGVCGSLGPHSGQSLRCSRGQRALGPAEHRPALRLLGRVTGGTLGPGRENPPSVLLAPRPGADFSPLSLQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXS8</accession>
    <name>A0A452GXS8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Lysoplasmalogenase TMEM86B</fullName>
            <ecNumber evidence="12">3.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Transmembrane protein 86B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the hydrolysis of the vinyl ether bond of choline or ethanolamine lysoplasmalogens, forming fatty aldehyde and glycerophosphocholine or glycerophosphoethanolamine, respectively and is specific for the sn-2-deacylated (lyso) form of plasmalogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine + H2O = a 2,3-saturated aldehyde + sn-glycerol 3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:22544"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:73359"/>
            <dbReference type="ChEBI" id="CHEBI:77287"/>
            <dbReference type="EC" id="3.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine + H2O = a 2,3-saturated aldehyde + sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:16905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:73359"/>
            <dbReference type="ChEBI" id="CHEBI:77288"/>
            <dbReference type="ChEBI" id="CHEBI:143890"/>
            <dbReference type="EC" id="3.3.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM86 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.2" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452GXS8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006921"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008024.1">
        <property type="protein sequence ID" value="ENSGAGP00000006921.1"/>
        <property type="gene ID" value="ENSGAGG00000005578.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047408">
        <property type="term" value="F:alkenylglycerophosphocholine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012506">
        <property type="entry name" value="TMEM86B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31885">
        <property type="entry name" value="GH04784P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31885:SF7">
        <property type="entry name" value="LYSOPLASMALOGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07947">
        <property type="entry name" value="YhhN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="202" mass="21647" checksum="8DF422B8C669FC8A" modified="2019-05-08" version="1">LQGGVGGSTLGRITPRSLGSHRCHKLLPFLASCGLYFALWLPEPSWVSAGVKGLPLLSLVWFLTAQAWGDGAWTPAARRVRWGLMFSSVGDFRLVWPQLFIPGMGPGSAMLWPCLGGPYVPAVGGYGALLAAMAWRALARPPPHLAVATGSLLFVASDLVLAWDHFCAPVPQARLIVMGTYYAAQGLIAAWAPRASPRWKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYM3</accession>
    <name>A0A452GYM3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc finger protein RFP-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GYM3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007328"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008471.1">
        <property type="protein sequence ID" value="ENSGAGP00000007328.1"/>
        <property type="gene ID" value="ENSGAGG00000005881.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16594">
        <property type="entry name" value="RING-HC_TRIM7-like_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12888">
        <property type="entry name" value="SPRY_PRY_TRIM7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="68"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="6">
        <location>
            <begin position="129"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="37563" checksum="A942471B80C0ADDC" modified="2019-05-08" version="1">MPLSLFPPATCSTPPPTFVASASFHQLISLPLVLTLHSLPHANTVTNCPEGAMAAENPVESLQEEATCPVCLEYFTEPVTVECGHNFCRDCISQCWEGSDTDISCPQCRETVQQRNLRPNRQLANMVEITKQLSLQKEERMRKLQTMNVTLDPDTAHPQLVLSEDRKSVRWGDTQQRLPDNPERFDYDPCVLGCEGFTLGRHCWEVEVGDGGCWALGVARESVGRKGQISCSPERGIWAVEWLGQFQALTSPVTPLCLNQAPSRIRVCLDCDQGQVTFIDAGDEAPIFTFPLGSVPGERIRPWLWVGGSRPQTLSESRPVEANALMGNTCRKLQKQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYM6</accession>
    <name>A0A452GYM6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">ATP binding cassette subfamily C member 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>17beta-estradiol 17-O-(beta-D-glucuronate)(in) + ATP + H2O = 17beta-estradiol 17-O-(beta-D-glucuronate)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:82961"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:60129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000007263"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008400.1">
        <property type="protein sequence ID" value="ENSGAGP00000007263.1"/>
        <property type="gene ID" value="ENSGAGG00000004641.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000293">
        <property type="entry name" value="ATP binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000007">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000074">
        <property type="entry name" value="Multidrug resistance-associated protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005292">
        <property type="entry name" value="MRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00957">
        <property type="entry name" value="MRP_assoc_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF176">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="228"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="627"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="681"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="744"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="767"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="852"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="18">
        <location>
            <begin position="17"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="292"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="18">
        <location>
            <begin position="730"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="945"/>
            <end position="1180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1308" mass="144063" checksum="32E841696890C267" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZ91</accession>
    <name>A0A452GZ91_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mothers against decapentaplegic homolog</fullName>
            <shortName evidence="12">MAD homolog</shortName>
            <shortName evidence="12">Mothers against DPP homolog</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">SMAD family member</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the dwarfin/SMAD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZ91"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007499"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008662.1">
        <property type="protein sequence ID" value="ENSGAGP00000007499.1"/>
        <property type="gene ID" value="ENSGAGG00000006006.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032924">
        <property type="term" value="P:activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009880">
        <property type="term" value="P:embryonic pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007369">
        <property type="term" value="P:gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060485">
        <property type="term" value="P:mesenchyme development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060395">
        <property type="term" value="P:SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10491">
        <property type="entry name" value="MH1_SMAD_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10985">
        <property type="entry name" value="MH2_SMAD_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000001">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.520.10:FF:000007">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.520.10">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013790">
        <property type="entry name" value="Dwarfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013019">
        <property type="entry name" value="MAD_homology_MH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036578">
        <property type="entry name" value="SMAD_MH1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703:SF42">
        <property type="entry name" value="MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703">
        <property type="entry name" value="SMAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56366">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51075">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MH1" evidence="14">
        <location>
            <begin position="10"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="MH2" evidence="15">
        <location>
            <begin position="274"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="207"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="467" mass="52264" checksum="05CD1D2BA0CE48A8" modified="2019-05-08" version="1">MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPSTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H015</accession>
    <name>A0A452H015_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Elbow/Noc family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H015"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007879"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009081.1">
        <property type="protein sequence ID" value="ENSGAGP00000007879.1"/>
        <property type="gene ID" value="ENSGAGG00000006276.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000129">
        <property type="entry name" value="Zinc finger protein 503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051520">
        <property type="entry name" value="Elbow/Noc_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022129">
        <property type="entry name" value="Tscrpt_rep_NocA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12522:SF3">
        <property type="entry name" value="ZINC FINGER PROTEIN 503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12522">
        <property type="entry name" value="ZINC-FINGER PROTEIN NOLZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12402">
        <property type="entry name" value="nlz1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="477"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="119"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="243"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="609" mass="61238" checksum="5795EBE4BDD39690" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H138</accession>
    <name>A0A452H138_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mab-21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H138"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008228"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009475.1">
        <property type="protein sequence ID" value="ENSGAGP00000008228.1"/>
        <property type="gene ID" value="ENSGAGG00000006534.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046903">
        <property type="entry name" value="Mab-21-like_nuc_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046906">
        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF48">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03281">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20266">
        <property type="entry name" value="Mab-21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mab-21-like nucleotidyltransferase" evidence="2">
        <location>
            <begin position="221"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Mab-21-like HhH/H2TH-like" evidence="3">
        <location>
            <begin position="310"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="424" mass="45087" checksum="F88292534AB6F523" modified="2019-05-08" version="1">MTSPEGGAVPRPAQPLTDLDFHSGARIAELNQLVQELSKRMARDPGIRATQELVVLKDFVFSLLGLVQQLDGRLPLANEYLLLSGGARQGSVDVDPGVLDRLAPGADYDVDYTLLVPVLQARGCPVTLDLRRCPPGHAWLALAPFGPEACHRWADCCHHHGGEAYLAPGLVSAWFSQALGTVADWTRAVPRPGGPAVERVAGQGGLVTLLLSHGAVRALYDVVPVVAFQGWPAAAREWLGRSHFWDGKLREEDVAGGFYLLPGSGPASGPAWRLSFARSELHLKKAVPPPLLQAYQAARAALSGAWGGRLGPYHLWTLVLWACERLPARYLGREENAAHCLLGLLDDMAAGLGAGACPHYFLPGCDRLAGGSEPGLARAVAAVRGDPAGHLRQAVERVKMAVGGDQSQGWLGARTPGFSISLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H247</accession>
    <name>A0A452H247_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="10">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452H247"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008528"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009815.1">
        <property type="protein sequence ID" value="ENSGAGP00000008528.1"/>
        <property type="gene ID" value="ENSGAGG00000006773.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd10361">
        <property type="entry name" value="SH2_Fps_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000212">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000089">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000020">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035849">
        <property type="entry name" value="Fes/Fps/Fer_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH2" evidence="11">
        <location>
            <begin position="54"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="155"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="416" mass="47413" checksum="97AEE89581623D81" modified="2019-05-08" version="1">PSASWQELELERPGASALETLRNHISGIFRPKYSVPPPLALLPEVQKPLCQQAWYHGAIPRAEVQELLLHSGDFLVRESQGKQEHVLSVLWDGQPRHFIIQACDDMYRLEGEGFRTIPLLIDHFLQTRQPITKKSGIVLAKPILKDKWVLNHEDVLLGERIGRGNFGEVFSGRLLADNTPVAVKSCRETLPPELKAKFLQEARILKQYSHPNIVKLIGICTQKQPIYIVMELVQGGDFLTFLRSEGQQLKVKELLKMTANAAAGMEYLESKHCIHRDLAARNCLVTEKNSLKISDFGMSREEEDGIYASTGGMKQIPVKWTAPEALNYGRYSSESDVWSFGILLWEAFSLGAIPYSNLSNTQTREAIEQGVRLDPPEQCPEDVYRLMQRCWEYDPRKRPSFSTIHQELISIRKRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2E8</accession>
    <name>A0A452H2E8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H2E8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008802"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010119.1">
        <property type="protein sequence ID" value="ENSGAGP00000008802.1"/>
        <property type="gene ID" value="ENSGAGG00000006965.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000130">
        <property type="entry name" value="Homeobox protein Hox-D12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051003">
        <property type="entry name" value="AP_axis_regulatory_Homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022067">
        <property type="entry name" value="HoxA13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45804:SF5">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45804">
        <property type="entry name" value="SEGMENTATION PROTEIN FUSHI TARAZU-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12284">
        <property type="entry name" value="HoxA13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="231"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="233"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="303" mass="34153" checksum="463685AA897FD55C" modified="2019-05-08" version="1">MKTSLILHPRWADTFMYVYEENSNENNQNKTPAMEALSGNCPASHCRDIITLGRHSSTIGTHQGSVYTDIPAPEAARQCPPPPASSNATLGYGYPFGGSYYGCRLSHSHNVNLQQKPCSYHPTEKYPEPSGSIPSEELPSRAKEFAFYPSFASSYQAVPGYLDVSVVPGISGHPEPRHDALLPMEGYQHWALSNGWDGQVYCSKEQSQSTHLWKSPFPDVVPLQPEVSSYRRGRKKRVPYTKIQLKELEKEYAASKFITKEKRRRISAATNLSERQVTIWFQNRRVKEKKVVSKSKTPHLHAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H329</accession>
    <name>A0A452H329_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H329"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008818"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010138.1">
        <property type="protein sequence ID" value="ENSGAGP00000008818.1"/>
        <property type="gene ID" value="ENSGAGG00000006981.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000010142.1">
        <property type="protein sequence ID" value="ENSGAGP00000008822.1"/>
        <property type="gene ID" value="ENSGAGG00000006981.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09485">
        <property type="entry name" value="LIM_Eplin_alpha_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000002">
        <property type="entry name" value="LIM domain and actin-binding 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028740">
        <property type="entry name" value="EPLIN_Lim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24206">
        <property type="entry name" value="OS06G0237300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="399"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="84"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="207"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="348"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="480"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="614"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="146"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="305"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="348"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="369"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="626"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="643"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="661"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="703"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="747"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="776" mass="87322" checksum="68B53D11EB2186C4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3V8</accession>
    <name>A0A452H3V8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntrophin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3V8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009133"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010487.1">
        <property type="protein sequence ID" value="ENSGAGP00000009133.1"/>
        <property type="gene ID" value="ENSGAGG00000007132.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016013">
        <property type="term" value="C:syntrophin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06801">
        <property type="entry name" value="PDZ_syntrophin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000080">
        <property type="entry name" value="Syntrophin gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015482">
        <property type="entry name" value="Syntrophin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10554:SF2">
        <property type="entry name" value="GAMMA-1-SYNTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10554">
        <property type="entry name" value="SYNTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="54"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="344" mass="38743" checksum="CAF2043BC748C9E1" modified="2019-05-08" version="1">QDFRTSRTHRICLLQDGNQEPFKVRLHLAKDILMIQEQDVICVSGEPFYSGERTVTIRRQTVGGFGLSIKGGAEHNIPVVISKISKEQRAELSGLLFIGDAILQINGINVRKCRHEEVVQVLRNAGEEVTLTVSFLKRAPAFLKLPLNEDCACVPSDQSSGTSSPLCDSGLHLNYHPNNTDTLSCSSWPTSPGLRWEKRWCDLRLIPLLHSRFSQYLPGSDLCRQNAFQVIAVDGVCSGIIQSVSAEDCMDWLQAIATNISNLTKHNVSIDQNLVKFTTVSQFIQCVETVILHTFQVNLTRCHIPVKLLPSCRGRMALWLRHWTRIQKILSELFQVVCLCLKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3X3</accession>
    <name>A0A452H3X3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Basolateral cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3X3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009201"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010565.1">
        <property type="protein sequence ID" value="ENSGAGP00000009201.1"/>
        <property type="gene ID" value="ENSGAGG00000007267.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008510">
        <property type="term" value="F:sodium:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003024">
        <property type="entry name" value="Na/HCO3_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF32">
        <property type="entry name" value="SODIUM-DRIVEN CHLORIDE BICARBONATE EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01232">
        <property type="entry name" value="NAHCO3TRSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="487"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="574"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="604"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="702"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="740"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="788"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="828"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="888"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="960"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="983"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="17">
        <location>
            <begin position="122"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="16">
        <location>
            <begin position="458"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="65"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1080"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1093"/>
            <end position="1113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1113" mass="125162" checksum="733A260E89362FE9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H5A8</accession>
    <name>A0A452H5A8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein transport protein sec16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus. Involved in peroxisome biogenesis. Regulates the transport of peroxisomal biogenesis factors PEX3 and PEX16 from the ER to peroxisomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">SEC16A and SEC16B are each present in multiple copies in a heteromeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the SEC16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H5A8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009691"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011131.1">
        <property type="protein sequence ID" value="ENSGAGP00000009691.1"/>
        <property type="gene ID" value="ENSGAGG00000007651.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09233">
        <property type="entry name" value="ACE1-Sec16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1030:FF:000003">
        <property type="entry name" value="Protein transport protein sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024340">
        <property type="entry name" value="Sec16_CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024298">
        <property type="entry name" value="Sec16_Sec23-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402:SF11">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC16B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402">
        <property type="entry name" value="RGPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12932">
        <property type="entry name" value="Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12931">
        <property type="entry name" value="TPR_Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="8 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec16 central conserved" evidence="15">
        <location>
            <begin position="286"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Sec16 Sec23-binding" evidence="14">
        <location>
            <begin position="451"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="867"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="926"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="898"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="934"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="952"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="962"/>
            <end position="986"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1066" mass="119132" checksum="DF6B716DB7185752" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6T9</accession>
    <name>A0A452H6T9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Basolateral cell membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H6T9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010404"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011942.1">
        <property type="protein sequence ID" value="ENSGAGP00000010404.1"/>
        <property type="gene ID" value="ENSGAGG00000008147.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014704">
        <property type="term" value="C:intercalated disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034109">
        <property type="term" value="P:homotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000095">
        <property type="entry name" value="immunoglobulin superfamily member 11 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052307">
        <property type="entry name" value="EJ_Adhesion_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44468:SF3">
        <property type="entry name" value="COXSACKIEVIRUS AND ADENOVIRUS RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44468">
        <property type="entry name" value="COXSACKIEVIRUS AND ADENOVIRUS RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0796">Tight junction</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="114"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="244"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="339" mass="37226" checksum="B04A05EF3483867B" modified="2019-05-08" version="1">MVEKAQGEKVTLPCTFTVSQEDQGPLDVEWVLIPTDNQKKEQTIIMYSVDRIYNDYYEAMNGRVQFTNPDPRSGDGSIDILNLKSADTGTYQCRVKKAPGVESQKIQLTVLVKPARTKCYIEGSQETGRDLTLKCVSQEGSPLLSYNWRKLTGTEELPATSLLNKDTGELSMKNVSEDYSGTYNCVAANRVGTDDCFVVLNVTPPINTAGTIAGAVIGTLLGLFLLAFLVFCCCKKRKEKKYEKEVHHDIREDVPPPKSRTSTARSYIGSNRSSLGSMSPSNMEGYAKTPYNQVPSEDFERSPGQNPNFPPSKVAAPNLSRMGAVPVMIPAQSKDGSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H798</accession>
    <name>A0A452H798_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Platelet-activating factor acetylhydrolase</fullName>
            <ecNumber evidence="3 11">3.1.1.47</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = a 1-O-alkyl-sn-glycero-3-phosphocholine + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30909"/>
            <dbReference type="ChEBI" id="CHEBI:36707"/>
            <dbReference type="EC" id="3.1.1.47"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.47" evidence="3 11"/>
    <dbReference type="AlphaFoldDB" id="A0A452H798"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010419"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011958.1">
        <property type="protein sequence ID" value="ENSGAGP00000010419.1"/>
        <property type="gene ID" value="ENSGAGG00000008159.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000011960.1">
        <property type="protein sequence ID" value="ENSGAGP00000010421.1"/>
        <property type="gene ID" value="ENSGAGG00000008159.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003847">
        <property type="term" value="F:1-alkyl-2-acetylglycerophosphocholine esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000062">
        <property type="entry name" value="Platelet-activating factor acetylhydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016715">
        <property type="entry name" value="PAF_acetylhydro_eukaryote"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10272">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10272:SF12">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03403">
        <property type="entry name" value="PAF-AH_p_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018169">
        <property type="entry name" value="PAF_acetylhydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="12">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="12">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR018169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR018169-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="399" mass="45387" checksum="2C2FD42129B082A8" modified="2019-05-08" version="1">MESKSSTKLYGIPEGKGPYSIGCTDLMTDYTIQGSFFRLYYPSQNGASSEETKWIPRKEYYRGLSDFLNMYRMLGETFFQYYVGSVTCPAKWNAAFKPGEKYPLIIFSHGLGAFRTIYSAICIEMASQGFIVAAVEHRDKSSSATYYHKENPGCEAQEEATENPNEEWIYYRKLKRSEQEFPLRNQQLQQRTEECIRALDLILDINNGNHVVNVLSLNFDWTLLKESIDTDRIAVMGHSFGAATAIKTLSKDTRFKCGIALDAWMLPLDDEVYSGFEQPLLFINSEKFQWVSNVLKMKKLYSTGKIRKMITIKGSVHQSFPDFTFLSGNFVGKIFRLKGEIEPNIAIDISNKASLAFLQKHLGLKKDFDQWDALVDGEGHNVIPGTNIDLPSTQPESLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9J3</accession>
    <name>A0A452H9J3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="12">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452H9J3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011434"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013090.1">
        <property type="protein sequence ID" value="ENSGAGP00000011434.1"/>
        <property type="gene ID" value="ENSGAGG00000008819.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05148">
        <property type="entry name" value="PTKc_Srm_Brk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000458">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000078">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 10" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="2 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="52"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="13">
        <location>
            <begin position="120"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="230"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="492" mass="56223" checksum="35BC25F48BFBDE0D" modified="2019-05-08" version="1">MEKHVRKHFSFLTSFWNKLWPNTGSADRPSQGYFDPDPVALRPDLEPDPVLPSAPLFSVLYDFRARSAEELSVSRGDKLCILKEEGEYVLARRLKGEPALGYIPANYVARINEETSHQPWYFRGISRNEAQQLLLSPPNQHGSFLIRDSESSQGEYSLSVRNHAQVSHFRICKGPDGGLYIQKGCPFPNLEELLTFYTVNWKVVQSPLLQPCIPKTPPVRDAWERPRSEFILRRKLGEGYFGEVWEGLWKNMVPVAIKIIKQADMKAEDFTKEIQNLKRLKHERLIQLHAVCSVGEPLYIVTELMRKGNLQSYLSSLEGRALSTLHLISIACQVADGMTYLEEQHIVHRDLAARNILVGDNLTCKIADFGLARLLKDDIYSTSGSTKIPVKWTAPEVANYRTYSLKSDVWSYGILLYEVFTYGQTPYEGMTNQETIQQISMGYRLPRPSTCPPEIYGVMLECWKGNAEERPTFLALREKLCSIYKRVHNSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HC13</accession>
    <name>A0A452HC13_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphotransferase</fullName>
            <ecNumber evidence="7">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:17826"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452HC13"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012305"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014091.1">
        <property type="protein sequence ID" value="ENSGAGP00000012305.1"/>
        <property type="gene ID" value="ENSGAGG00000009463.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00242"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="9">
        <location>
            <begin position="33"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="136"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="209" mass="21760" checksum="317DBF1128AD3F39" modified="2019-05-08" version="1">QGTRPPSGLVHPVRRLLLPEHGGCLARRTGNSLRPLCLSLETLHRVKSRMLEAMERGLSRRTHSQATVRMLPAFIRSTPDGTERGEFLVLELGDTHLRVLLMELSGDTDQRVTMKERCFPVPDAIAQGPGEKVWGQGTLAPAGWAGDGTAAGSKPASSLGPTQLAPGRGSLGAGQPGDGGSCECCASTLEPGAGRGAGLGLGEGQPLPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDC8</accession>
    <name>A0A452HDC8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="10">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits.</text>
    </comment>
    <comment type="function">
        <text evidence="10">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="10">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family. DDX51/DBP6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452HDC8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012804"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014678.1">
        <property type="protein sequence ID" value="ENSGAGP00000012804.1"/>
        <property type="gene ID" value="ENSGAGG00000009833.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000014681.1">
        <property type="protein sequence ID" value="ENSGAGP00000012807.1"/>
        <property type="gene ID" value="ENSGAGG00000009833.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17956">
        <property type="entry name" value="DEADc_DDX51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001539">
        <property type="entry name" value="ATP-dependent RNA helicase DDX51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="302"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="540"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="38"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="728" mass="82246" checksum="F646DE9483A9B1C2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HE19</accession>
    <name>A0A452HE19_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HE19"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013091"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014994.1">
        <property type="protein sequence ID" value="ENSGAGP00000013091.1"/>
        <property type="gene ID" value="ENSGAGG00000010040.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033173">
        <property type="term" value="P:calcineurin-NFAT signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07881">
        <property type="entry name" value="RHD-n_NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000040">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000001">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008366">
        <property type="entry name" value="NFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533">
        <property type="entry name" value="NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533:SF5">
        <property type="entry name" value="NUCLEAR FACTOR OF ACTIVATED T-CELLS, CYTOPLASMIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01789">
        <property type="entry name" value="NUCFACTORATC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHD" evidence="11">
        <location>
            <begin position="397"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="180"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="702" mass="77354" checksum="20C1644044A24AD3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFU4</accession>
    <name>A0A452HFU4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal fusion of the chorion and the allantois during placenta development. Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFU4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013679"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015658.1">
        <property type="protein sequence ID" value="ENSGAGP00000013679.1"/>
        <property type="gene ID" value="ENSGAGG00000010431.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000015660.1">
        <property type="protein sequence ID" value="ENSGAGP00000013681.1"/>
        <property type="gene ID" value="ENSGAGG00000010431.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19350">
        <property type="entry name" value="wnt_Wnt7b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013300">
        <property type="entry name" value="Wnt7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF73">
        <property type="entry name" value="PROTEIN WNT-7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01891">
        <property type="entry name" value="WNT7PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="353" mass="39705" checksum="8E1051327EE46B6D" modified="2019-05-08" version="1">MLLFSSRSVLLSVYYPQIFLILTSGSYLALSSVVALGANIICNKIPGLAPRQRAICQSRPDAIIVIGEGAQMGINECQYQFRYGRWNCSALGEKTVFGQELRVGSREAAFTYAITAAGVAHAVTAACSQGNLSNCGCDREKQGYYNQEEGWKWGGCSADIRYGIEFSRKFVDAREIKKNARRLMNLHNNEAGRKVLEERMKLECKCHGVSGSCTTKTCWTTLPKFREIGYILKEKYNAAVQVEVVRASRLRQPTFLKIKQIRSYQKPMETDLVYIEKSPNYCEEDASTGSVGTQGRLCNRTSPNADGCDMMCCGRGYNTHQYTKVWQCNCKFHWCCFVKCNTCSERTEVFTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGE9</accession>
    <name>A0A452HGE9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGE9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013930"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015950.1">
        <property type="protein sequence ID" value="ENSGAGP00000013930.1"/>
        <property type="gene ID" value="ENSGAGG00000010613.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12551">
        <property type="entry name" value="RRM_II_PABPN1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000311">
        <property type="entry name" value="polyadenylate-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF27">
        <property type="entry name" value="EMBRYONIC POLYADENYLATE-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="169"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="111"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="289" mass="31396" checksum="084ED375B344198B" modified="2019-05-08" version="1">MKVGWGKACSTRAGLRTSCCGCPGLGQAGLWLQGCALAFGSRPSISDLAQVFQCLKQWPSHKTLATPKPEIEEAPSRPSNQTAGAETGLGPGVSSCRAAPLCTGFSRSRPLGFGAQELEAIKAKVREMEEEDERLKELQAEAKNHLILRSQAGIPFPRTAEEKIEADQRSIYVGNVDYGGTAEELESHFNSCGQINRVTILCDKFSGHPKGYAYIEFEDKSSVKAAVELDKSMFRGRVIKVLPKRTNMPGISTTDRGGSRGRFPSRGGLSQRANVYGALRARSRGRVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHM5</accession>
    <name>A0A452HHM5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleoporin NUP42</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Nucleoporin-like protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HHM5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014392"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016463.1">
        <property type="protein sequence ID" value="ENSGAGP00000014392.1"/>
        <property type="gene ID" value="ENSGAGG00000010937.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051767">
        <property type="entry name" value="Nucleoporin_NUP42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527">
        <property type="entry name" value="NUCLEOPORIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="3" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="23"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="236"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="418" mass="44066" checksum="4E69BD63B8EA4E1A" modified="2019-05-08" version="1">MTICHFFLQGRCRFGERCWNEHPRGGGRSGPAAPPSQGSGRGGWGAHNQRYTNVVQPSNFSKPTAWGGSRDDGKAFFGPSIDSGAPGSGNKSSGFSQNRFSALSSEQNIEEKLLEGIIKDMEVWESSGQWMFSCYSPMKEKPNISGFPDFSPEELHLEYYNCRANNNIQNYINSVQQLVNQWRNRLLMLKTLNTSTKAALVSELKNVVSQPLPAFGFGGQQTSTFGSSGFPLSSSSSRSASSSSFKSNTELVSPSSGSTTAFGSSSMVPNPSALGVTSSARSAPSIGFGSQPASSAASFSFKKPETTGGFGASGFSGFGSSSAVNLSSTTSLPAFGAVNAAVAAPSSEPGNSLFGRSLNAFGYNVAPAPPAATSIVTSDKLFTPKTDLSPEELKQFEAKKFTLGKIPLKPPPIELLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHR8</accession>
    <name>A0A452HHR8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HHR8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014441"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016520.1">
        <property type="protein sequence ID" value="ENSGAGP00000014441.1"/>
        <property type="gene ID" value="ENSGAGG00000010980.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07660">
        <property type="entry name" value="BAR_Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000003">
        <property type="entry name" value="arfaptin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010504">
        <property type="entry name" value="AH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030798">
        <property type="entry name" value="Arfaptin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141:SF3">
        <property type="entry name" value="ARFAPTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141">
        <property type="entry name" value="ARFAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AH" evidence="7">
        <location>
            <begin position="205"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="122"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="425" mass="46756" checksum="04A19D88334ECC61" modified="2019-05-08" version="1">MASSAPRREVTCLLCTQLARKVQKETDTWRIHRDITENPLFVLGWPGAAGPGCPWGLTPHSRGQSEPRAAPSPPGLCSCPFQDLQQVMVSGPNLNETSIVSGGYGGTAEGIIPTSTVKGSFVTSHSSRGPSISPSESAASRIASQADASAGSGLHLPHPGPLAAGDEAARGIAVEKFDIVKKWGINTYKCTKQLISERFGRGSRTVDLELETQIELLRDTKRKYECVLQLARALTHHFYSLVQTQHALGDAFSDLSQKSPELQEEFGCNAETQKLLCKNGETLLGAVNFFVSSINTLVNKTMEDTLMTVKQYETARLEYDAYRTDLEELSIGPRDANTLCRLDAAQSHFQSHKGKYEKLRADVAIKLKFLEENKIKVMHKQLLLFHNAVSAYFAGNQQQLAQTLRQFNIKLKTPGAEKPSWLEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIS0</accession>
    <name>A0A452HIS0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HIS0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014789"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016899.1">
        <property type="protein sequence ID" value="ENSGAGP00000014789.1"/>
        <property type="gene ID" value="ENSGAGG00000011204.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="99"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="553" mass="63154" checksum="D44032884D9DD96D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKC2</accession>
    <name>A0A452HKC2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HKC2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015344"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017525.1">
        <property type="protein sequence ID" value="ENSGAGP00000015344.1"/>
        <property type="gene ID" value="ENSGAGG00000011572.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003655">
        <property type="entry name" value="aKRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF142">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50806">
        <property type="entry name" value="KRAB_RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB-related" evidence="2">
        <location>
            <begin position="7"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="10"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="97" mass="11079" checksum="F56C853F3265C67A" modified="2019-05-08" version="1">SLPRPAPGPVTFEDVAVYFTREEWALLDPTQRALYRAIMQENYENVTSLGFPVSKPDVISQLEQGEEPWMRGPFWPFFSLPCFEVDPFVLSISSIVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLA6</accession>
    <name>A0A452HLA6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Tyrosine-protein kinase Mer</fullName>
            <ecNumber evidence="3">2.7.10.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Proto-oncogene c-Mer</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Receptor tyrosine kinase MerTK</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 33">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Interacts (upon activation) with TNK2; stimulates TNK2 autophosphorylation. Interacts (via N-terminus) with extracellular ligands LGALS3, TUB, TULP1 and GAS6. Interacts with VAV1 in a phosphotyrosine-independent manner. Interacts with TIMD4; this interaction enhances TIMD4-mediated efferocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452HLA6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015720"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017949.1">
        <property type="protein sequence ID" value="ENSGAGP00000015720.1"/>
        <property type="gene ID" value="ENSGAGG00000010943.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000902">
        <property type="entry name" value="MER proto-oncogene, tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001050">
        <property type="entry name" value="MER proto-oncogene, tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000834">
        <property type="entry name" value="Proto-oncogene tyrosine-protein kinase MER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000089">
        <property type="entry name" value="Tyrosine-protein kinase receptor TYRO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000296">
        <property type="entry name" value="Tyrosine-protein kinase receptor TYRO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000111">
        <property type="entry name" value="Tyrosine-protein kinase receptor TYRO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF257">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE MER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 25" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 27" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="514"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="29">
        <location>
            <begin position="105"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="29">
        <location>
            <begin position="209"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="30">
        <location>
            <begin position="298"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="30">
        <location>
            <begin position="398"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="599"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="631"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00066004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00070212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00077110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00082356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1009" mass="112155" checksum="4A9863C3FAAF2E42" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMC8</accession>
    <name>A0A452HMC8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calmodulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HMC8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016145"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018433.1">
        <property type="protein sequence ID" value="ENSGAGP00000016145.1"/>
        <property type="gene ID" value="ENSGAGG00000012120.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019007153" description="EF-hand domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="50"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="163" mass="18824" checksum="8A0DA40168C24FFD" modified="2019-05-08" version="1">MTSFFAFILLLQSVVFLWENLRLLIIQWPFYCKEILELFKHFAFVEFTPKKVFSVFDTNGNGVITAKELVAVMWSFGQNPTEAELQDMILEVDDSAHEEDIREAFYVFDKDVSTYITTAKLHHVMTNFGEKLADEVDEIIREADIDDDGQVNGEQLVQMVTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMT5</accession>
    <name>A0A452HMT5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA (guanosine(18)-2'-O)-methyltransferase TARBP1</fullName>
            <ecNumber evidence="10">2.1.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">TAR RNA-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in a subset of tRNA. Selectively mediates Gm18 methylation of tRNAGln-TTG/CTG and tRNASer-TGA/GCT. Gm18 modification can enhance the stability of modified tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>guanosine(18) in tRNA + S-adenosyl-L-methionine = 2'-O-methylguanosine(18) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20077"/>
            <dbReference type="Rhea" id="RHEA-COMP:10190"/>
            <dbReference type="Rhea" id="RHEA-COMP:10192"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74445"/>
            <dbReference type="EC" id="2.1.1.34"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:20078"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer and homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.34" evidence="10"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016272"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018574.1">
        <property type="protein sequence ID" value="ENSGAGP00000016272.1"/>
        <property type="gene ID" value="ENSGAGG00000012102.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141100">
        <property type="term" value="F:tRNA (guanine(18)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18091">
        <property type="entry name" value="SpoU-like_TRM3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000010">
        <property type="entry name" value="probable methyltransferase TARBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025806">
        <property type="entry name" value="TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056921">
        <property type="entry name" value="TARBP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045330">
        <property type="entry name" value="TRM3/TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044748">
        <property type="entry name" value="Trm3/TARBP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029:SF11">
        <property type="entry name" value="METHYLTRANSFERASE TARBP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029">
        <property type="entry name" value="RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25050">
        <property type="entry name" value="TARBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51624">
        <property type="entry name" value="SAM_MT_TRMH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TARBP1" evidence="14">
        <location>
            <begin position="198"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="13">
        <location>
            <begin position="1402"/>
            <end position="1543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1558" mass="175801" checksum="B5547D400F771E6C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNA9</accession>
    <name>A0A452HNA9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HNA9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016465"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018792.1">
        <property type="protein sequence ID" value="ENSGAGP00000016465.1"/>
        <property type="gene ID" value="ENSGAGG00000012320.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003707">
        <property type="term" value="F:nuclear steroid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005496">
        <property type="term" value="F:steroid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048477">
        <property type="term" value="P:oogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF41">
        <property type="entry name" value="MEMBRANE PROGESTIN RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0896">Oogenesis</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0754">Steroid-binding</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="352" mass="40791" checksum="D03175134065969A" modified="2019-05-08" version="1">MATIVPEKLTRLFINVQQLWQVPRLLEMAFPSSPCTVSTSDVPKVFWKPYIHTGYRPLNQTWKYYFLTLFQQHNEAVNVWTHLVAALILLLRFRRLSQTVDFVSDPHAHPLFIIVIASITYLTFSTLAHLLQAKSEFWHYSFFFMDYVGVAIYQYSSALVHYYYAIEPDWHAKIMGFYMPGAVLLAWLSCAGSCYAKYRYHQLPRHLSRLCQEMPLGLAYALDISPVIHRLCTAQPSEQADPALLYHKCQVLFFLISAFFFSYPYPEKWFPGKCHFFGQGHQIFHVFLVLCTLAQVQAVALDYESRREIYTSLHGDQTHNFSALCFFTVACCLLTAAYMTSKVKCKLNCKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNR5</accession>
    <name>A0A452HNR5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HNR5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016652"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019010.1">
        <property type="protein sequence ID" value="ENSGAGP00000016652.1"/>
        <property type="gene ID" value="ENSGAGG00000012449.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000017">
        <property type="entry name" value="potassium voltage-gated channel subfamily KQT member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000016">
        <property type="entry name" value="Putative potassium voltage-gated channel subfamily KQT member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1910">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003937">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003947">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013821">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735:SF7">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03520">
        <property type="entry name" value="KCNQ_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01461">
        <property type="entry name" value="KCNQ2CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01459">
        <property type="entry name" value="KCNQCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="329"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="93"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="18">
        <location>
            <begin position="310"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="17">
        <location>
            <begin position="492"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="470"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="492"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF03520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="727" mass="80962" checksum="0F33552D9EF27EFE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNY0</accession>
    <name>A0A452HNY0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000016676"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019041.1">
        <property type="protein sequence ID" value="ENSGAGP00000016676.1"/>
        <property type="gene ID" value="ENSGAGG00000012447.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13284">
        <property type="entry name" value="PH_OSBP_ORP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000074">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000003">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF194">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="57"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="291"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="764" mass="85853" checksum="A533D6FD02D7FA0A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQJ0</accession>
    <name>A0A452HQJ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Antizyme inhibitor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ornithine decarboxylase antizyme inhibitor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Antizyme inhibitor (AZI) protein that positively regulates ornithine decarboxylase (ODC) activity and polyamine uptake. AZI is an enzymatically inactive ODC homolog that counteracts the negative effect of ODC antizymes (AZs) OAZ1, OAZ2 and OAZ3 on ODC activity by competing with ODC for antizyme-binding. Inhibits antizyme-dependent ODC degradation and releases ODC monomers from their inactive complex with antizymes, leading to formation of the catalytically active ODC homodimer and restoring polyamine production.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer. Interacts with OAZ1 and OAZ3; this interaction disrupts the interaction between the antizyme and ODC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the Orn/Lys/Arg decarboxylase class-II family. ODC antizyme inhibitor subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQJ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017274"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019703.1">
        <property type="protein sequence ID" value="ENSGAGP00000017274.1"/>
        <property type="gene ID" value="ENSGAGG00000012860.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042978">
        <property type="term" value="F:ornithine decarboxylase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004586">
        <property type="term" value="F:ornithine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042177">
        <property type="term" value="P:negative regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902269">
        <property type="term" value="P:positive regulation of polyamine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033387">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06831">
        <property type="entry name" value="PLPDE_III_ODC_like_AZI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.37.10:FF:000008">
        <property type="entry name" value="antizyme inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000010">
        <property type="entry name" value="Antizyme inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031178">
        <property type="entry name" value="Azin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482:SF7">
        <property type="entry name" value="ANTIZYME INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="ARGININE/DIAMINOPIMELATE/ORNITHINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="3 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="12">
        <location>
            <begin position="46"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600183-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="450" mass="49967" checksum="4C6D723F5F22C37C" modified="2019-05-08" version="1">MKGFLEDANYSVGLLDEGATLGDVIDNYVYEHTLTGKNAFFVGDLGKLLKKHNQWQNVMAPVKPFYAVKCNSSPGVLEILAALGIGFACSSKSEMTLVQDLGIAPENIIYTNPCKQASQIKYAAKTGVNIMTCDNESELKKIARNHPSAKLLLHIATEDISGAEEMNMKFGTMLKSCRHLMECAKELEVQIVGVKFHISSSCKEPQVYIHAISDARCVFDMAEEFGFKMNMLDIGGGFTGSELQLEEVNHVISPLLDVYFPEESGVNVIAEPGCYYVSSAFTLAVNIIAKKAVEYDKVLPSGMEQPRSDDEPIFMYYMNDGVYGSFASKLSEKFNTIPEVHKKYKEDEPLFASSLWGPSCDELDQIVENCLLPELSVGDWLIFDNMGSGTLGQQSAFNDFQRPPVYYMMSFNDWYEMQDAGITSDTLMKNFFFVPSCIQLSPEDSFSTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQR2</accession>
    <name>A0A452HQR2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQR2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017386"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019834.1">
        <property type="protein sequence ID" value="ENSGAGP00000017386.1"/>
        <property type="gene ID" value="ENSGAGG00000012936.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026507">
        <property type="entry name" value="PIRC1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20899">
        <property type="entry name" value="PIERCE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20899:SF4">
        <property type="entry name" value="PIERCER OF MICROTUBULE WALL 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14892">
        <property type="entry name" value="PIRC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="112" mass="12666" checksum="68927A0C8650F911" modified="2019-05-08" version="1">TLDRDPECMKIKSVQLPSCANPGNPVFSCMLDPKTLTTNNFLTNPQLLLFKTTSSEYGAIPPTSQMVPCTYHPKDETFTKHLLTCGLTQHNYINTAIDRSRVYDYPNMQHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HS84</accession>
    <name>A0A452HS84_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Palmitoyltransferase</fullName>
            <ecNumber evidence="14">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:36684"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A452HS84"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017907"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020428.1">
        <property type="protein sequence ID" value="ENSGAGP00000017907.1"/>
        <property type="gene ID" value="ENSGAGG00000013282.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349">
        <property type="entry name" value="ANKYRIN REPEAT AND LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349:SF3">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="16">
        <location>
            <begin position="102"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="294"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="359"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="421"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="442"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="583"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="615"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="638"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="672"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="688"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="719" mass="78162" checksum="96599DD5714605BE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HU95</accession>
    <name>A0A452HU95_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the opioid neuropeptide precursor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HU95"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018684"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021300.1">
        <property type="protein sequence ID" value="ENSGAGP00000018684.1"/>
        <property type="gene ID" value="ENSGAGG00000013833.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001515">
        <property type="term" value="F:opioid peptide activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031628">
        <property type="term" value="F:opioid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007600">
        <property type="term" value="P:sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002367">
        <property type="entry name" value="Nociceptin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006024">
        <property type="entry name" value="Opioid_neupept"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11438:SF2">
        <property type="entry name" value="PREPRONOCICEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11438">
        <property type="entry name" value="PROENKEPHALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01160">
        <property type="entry name" value="Opiods_neuropep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01028">
        <property type="entry name" value="OPIOIDPRCRSR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01031">
        <property type="entry name" value="ORPHNNPRCRSR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01252">
        <property type="entry name" value="OPIOIDS_PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="5" id="KW-0555">Opioid peptide</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019091274" evidence="6">
        <location>
            <begin position="20"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="230" mass="26274" checksum="5CE2B602131CE66F" modified="2019-05-08" version="1">MGALLWDLLLLCLFAHVLGDCQRDCLSCNRHLYNQQDNFNVLVCIMECEGKAFSSTTWELCTKVVGKSSIQVNADSLEEDSYQPLEMDDSGLFRGSRKHFNDLTKVVDLSKMEDEKRVSKVSSLIRQREVDEGVSNGSETPLGDFQEQPKDISKRLGGFLKGKYSYRQVLEPTVQGVQKRYGGFIGVRKSARKWNNQKRFSEFLKQYLGMSPRSSEYDSLTNDLNEQNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HV41</accession>
    <name>A0A452HV41_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HV41"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018998"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021657.1">
        <property type="protein sequence ID" value="ENSGAGP00000018998.1"/>
        <property type="gene ID" value="ENSGAGG00000014056.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08321">
        <property type="entry name" value="Pyrin_ASC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004020">
        <property type="entry name" value="DAPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02758">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01289">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50824">
        <property type="entry name" value="DAPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrin" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="146" mass="16374" checksum="50B38F8FBBFFCFBE" modified="2019-05-08" version="1">MAKTLRDHLFDTIRELGESDMKAFKDKLNNFQPKPGYNRIKWGELEKADAVAITRLLVNYYKEDYAVEVARKMLEDMDKKNLAHNLFQATGKAPGGTQQTPAPTSSTRTRVTDVAAPMLMEYRPGSSRAGDSGRVSRRPGFPAHCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVV1</accession>
    <name>A0A452HVV1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000019262"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021944.1">
        <property type="protein sequence ID" value="ENSGAGP00000019262.1"/>
        <property type="gene ID" value="ENSGAGG00000014232.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19762">
        <property type="entry name" value="Bbox2_TRIM7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16594">
        <property type="entry name" value="RING-HC_TRIM7-like_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12888">
        <property type="entry name" value="SPRY_PRY_TRIM7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="48"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="236"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="163"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="518" mass="57852" checksum="3617C31F0EB7518F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXU6</accession>
    <name>A0A452HXU6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HXU6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019976"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022759.1">
        <property type="protein sequence ID" value="ENSGAGP00000019976.1"/>
        <property type="gene ID" value="ENSGAGG00000014718.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071846">
        <property type="term" value="P:actin filament debranching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034316">
        <property type="term" value="P:negative regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011171">
        <property type="entry name" value="GMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249:SF4">
        <property type="entry name" value="GLIA MATURATION FACTOR GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249">
        <property type="entry name" value="GLIAL FACTOR NATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="139" mass="15216" checksum="383D6AB1ACD5E55B" modified="2019-05-08" version="1">MVRLSIASPSDSLVVCDVDPALTEKLRKFRFRKETNNAAILMKIDKEQQLVVLEEEFQVGTFHSLLPATFGKPSSVKGPAGSGLMPSSSQTPLCHRAPTASVGLEPSSSCFQSPAPCHWAWRSTVGGYEVQRWQSDCLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXY9</accession>
    <name>A0A452HXY9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HXY9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020018"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022806.1">
        <property type="protein sequence ID" value="ENSGAGP00000020018.1"/>
        <property type="gene ID" value="ENSGAGG00000014753.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028042">
        <property type="entry name" value="DUF4639"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34438:SF1">
        <property type="entry name" value="CHROMOSOME 2 OPEN READING FRAME 81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34438">
        <property type="entry name" value="SI:DKEY-97L20.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15479">
        <property type="entry name" value="DUF4639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="489" mass="54008" checksum="0DDAB167AF16A488" modified="2019-05-08" version="1">MTSRDRVPLSKSRAEKSRPPTVPIPQVDIVPGRLSESEWISLVTMEEGEDGVGDILEGMINQVMDECYKVYLARQCIPFTISQARDAILQIAEWRFLVRDEGEATVEEDTTWQEEEEPVACVTDSWAQGSVPVMRTSLTLFPQEREVSLDKLPEETVFHEETRPSTGSPEVPATPPSELPSQERGVKSSKSSKPQNLLPQPAPPPAPKTRKAYRAHRGLLRSASLKSMARPLQETEKELLRQQLSQTLPEESPQEDLGSLHQLLPMSCSNLLKIQMGRPPNIKGVTYDEFGTVIAMPKLDPARLPKCWIQPQVEVLDPDVEAKRQEVLRTVSGRGQRSTKSHSRAAQGPGLGELINAKLLQDTGPMLSGGLAQAFCPSHADKRAQHLPSTGKSLEPSSHVFWKPSNLLDSIELAPGVAIRDSSSVKCGPHHGALYEEGEEGVFQGKLRPIRPRVPLPTVAVEQLIRDHMPHVRPMALLRSLLSSGPVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HY24</accession>
    <name>A0A452HY24_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HY24"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020048"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022836.1">
        <property type="protein sequence ID" value="ENSGAGP00000020048.1"/>
        <property type="gene ID" value="ENSGAGG00000014774.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001782">
        <property type="term" value="P:B cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030865">
        <property type="term" value="P:cortical cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045579">
        <property type="term" value="P:positive regulation of B cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043029">
        <property type="term" value="P:T cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21505">
        <property type="entry name" value="PPP2R3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000091">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.220:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041534">
        <property type="entry name" value="EF-hand_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039865">
        <property type="entry name" value="PPP2R3C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12085">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12085:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17958">
        <property type="entry name" value="EF-hand_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PP2A regulatory subunit B'' EF-hand" evidence="9">
        <location>
            <begin position="179"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="450" mass="52828" checksum="0786F26282ADDC9F" modified="2019-05-08" version="1">MEWKQALRGRVAACIGSKKSEQELKDEEMELFTKYYMEWKGGRRSVNTSYMNIPRFYYRLPAEDEVLLQKLREESRAVFLQRKSRELLDNEELQNLWFLLDKHQTPPLIGEEAMINYESFLKVGEKAGPKCKQFFTAKVFAKLLHNDPYGRISIMQFFNYVMRKVWLHQTRIGLSLYDVAGQGYLRESDLENYILELIPTLPQLDGLEKSFYSFYVCTAVRKFFFFLDPLRTGKIKIQDILACSFLDDLLELRDEELSKESQETNWFSAPSALRVYGQYLNLDKDHNGMLSKEELSRYGTGTLTNIFLDRVFQECLTYDGEMDYKTYLDFVLALENRKEPAALQYIFKLLDIENKGYLNVFSLNYFFRAIQEQMKIHGQEPVSFQDVKDEIFDMVKPKDPYKISLQDLINSSQGDTVTSILIDLNGFWTYENREVLVASDNDNTVDIDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0I1</accession>
    <name>A0A452I0I1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional adapter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I0I1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020947"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023861.1">
        <property type="protein sequence ID" value="ENSGAGP00000020947.1"/>
        <property type="gene ID" value="ENSGAGG00000015399.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070461">
        <property type="term" value="C:SAGA-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02335">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000185">
        <property type="entry name" value="Transcriptional adapter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000170">
        <property type="entry name" value="Transcriptional adapter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000011">
        <property type="entry name" value="Transcriptional adapter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041983">
        <property type="entry name" value="ADA2-like_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016827">
        <property type="entry name" value="Ada2/TADA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056267">
        <property type="entry name" value="Ada2b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055141">
        <property type="entry name" value="TADA2A_B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF63">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 2-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22941">
        <property type="entry name" value="TADA2A-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24533">
        <property type="entry name" value="Tri-helical_Ada2b_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25299">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025024">
        <property type="entry name" value="Transcriptional_adaptor_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="12">
        <location>
            <begin position="4"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="13">
        <location>
            <begin position="65"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="11">
        <location>
            <begin position="67"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="306"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR025024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="420" mass="48359" checksum="945B4C6CD5C40F42" modified="2019-05-08" version="1">MAELGKKYCVYCLAEVSSLRFRCTECADIELCPDCFSAGAEIGPHRRWHGYQLVDGGRFTLWGAEAEGGWSSREEQLLLDAIEQFGFGNWEDMAAHVGASRTPQEVMEHYVSMYIHGNLGKACIPDSIPNRVTDHTCPSGGPLSPSLTTPLPPLDISVAEQQQLGYMPLRDDYEIEYDQDAETLISGLSVNYDDDDVEIELKRAHVDMYVRKLKERQRRKNIARDYNLVPAFLGKDKKDKEKAPKRKITKEEKELRLKLRPLYQFMSCKEFEDFFENMHKERVLRAKIRELQRYRRNGITKMEESAEYEAARHKREKRKENKNIANSKRGKEDGKDGEFAAIENLPGFELLSDREKVLCSSLNLSPARYVTVKTIIIKDHLQKRQGIPSKSRLPSYLDKVLKKRILNFLTESGWISRDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2B9</accession>
    <name>A0A452I2B9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I2B9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021634"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024642.1">
        <property type="protein sequence ID" value="ENSGAGP00000021634.1"/>
        <property type="gene ID" value="ENSGAGG00000015877.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000594">
        <property type="entry name" value="Myosin light chain kinase isoform-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342:SF20">
        <property type="entry name" value="MYOSIN LIGHT CHAIN KINASE, SMOOTH MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="235"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="38358" checksum="C59FD389324A8AC9" modified="2019-05-08" version="1">MRQILEGIQYVHRQSIVHLDLKPENIVCVSTSSHWIKIIDFGLARRLDPNSPVKVMHGTAEFMAPEVIAFEPVGFTTDMWSIGVICYILLSGDSPFQGNSDMETLHNITAAQWEFDEETFSEISQEAKDFISQLLQKDSRCRLPSDQALAHPWLQKTEQSDTKVLSKERMRRFLARQKWQKTGNAVVALQRMSRLAHKLDDPMTTPSAREKGELGHSQEEDQSFTFLPQQIDQGPSLEPLKDQVAVEGSSTCLQWHIEGFPHPEVTWLWNDAPVQECPQLPIDCEENGSRSLAIARLSVEDTGHCVCSAGGEVEYSARLMVQRGSNTQTLNAEEGPGTDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2X4</accession>
    <name>A0A452I2X4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I2X4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021862"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024904.1">
        <property type="protein sequence ID" value="ENSGAGP00000021862.1"/>
        <property type="gene ID" value="ENSGAGG00000016047.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36867">
        <property type="entry name" value="MCG131172, ISOFORM CRA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36867:SF1">
        <property type="entry name" value="RIKEN CDNA 2610318N02 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="285" mass="31974" checksum="093DE028B551526E" modified="2019-05-08" version="1">METLDEFDNEYPLSMSFCKLISPEDFEVQSLSYTEQCLQELYTNMERSPEICERVMRKRKQMEKEAAGLASFLKAKFFWTLQGEMNYCNYVGIAEMQEKVVQLKRDMQKVNNYAQAAKAVKMRRPRQVTGKKQALEGGFCPSWARTSGSTAPPKFTMPRVFGPFPELMSKQMDRATASNTDLKHYWAGVSTMNQKSMIDLHPIVLNPGGFHASLQAGNHINKLRYTSLSRPPGNSPNPSPQKAGPSDMKSTPSVFDTPMLSRFQGGSEDDMDNEKPGPSSSPKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I315</accession>
    <name>A0A452I315_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I315"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021907"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024957.1">
        <property type="protein sequence ID" value="ENSGAGP00000021907.1"/>
        <property type="gene ID" value="ENSGAGG00000016082.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05716">
        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052314">
        <property type="entry name" value="Immune_rcpt_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16423">
        <property type="entry name" value="TREM-LIKE TRANSCRIPT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16423:SF6">
        <property type="entry name" value="TRIGGERING RECEPTOR EXPRESSED ON MYELOID CELLS 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019419841" description="Ig-like domain-containing protein" evidence="5">
        <location>
            <begin position="33"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="8"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="134"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="348" mass="39168" checksum="09B5780396378F77" modified="2019-05-08" version="1">MGYSSNAPSVGLKTGMELRFLLLLLCISGFEAQEFDAVVSRVEGQRLIVECRYNYWDYSATPKTWCQLGDEKCFPLVSTSYTSYGYYNKVTFGRATIEDHTGNGIVTITMEKLQVQDSGVYWCARYYPPNTMYPLMTIKLDVSKVQEYDAIEGQSLSVQCPYNTQDYKEVKKAWCRWKGQNRCAVLVNTDGRYYGYQNRAQKGRARIHDDTQKGIVTVTMENLQVDDAGVYWCAHYKPPSFYLIFEVKLAVTKATTTSVVPVTSSTGHTSLVLSTASAGVTPEDSSSLNSRSTFLWLAVGFLINKTLLAVMIALPVRRRNRLRGSTQAPTNLEFMVRNDSLSLKTKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7U6</accession>
    <name>A0A452I7U6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I7U6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023705"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027004.1">
        <property type="protein sequence ID" value="ENSGAGP00000023705.1"/>
        <property type="gene ID" value="ENSGAGG00000017360.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15231">
        <property type="entry name" value="7tmA_OR5V1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="49"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="322" mass="36060" checksum="6B92A808DD7DC40C" modified="2019-05-08" version="1">LRERLISKLAMENQTTPTEFILSGFSKLRGSRFLLLGVISIIYIFTLLGNMLILLLSLVDPYLRTPMYLFLRNLSLLDIFQTTTTIPQMLQHLLSGRSSISYGSCMAQLYFFLLFVGAEGILLATMAYDRYVAICNPLLYTVLMSTKLCSTLVAASWLIGSFNAAVHTVLTIRLSFCGVNRLRYFYCDIPPLLALSCGDVSLNVIVTVVSSLFLGWGPSLCIILSYVHIVFRILKMQSSQGRRKAFSTCASHLTVVLLYYGSCIFTYIRPISSYSLDKDRLISLLYSLVTPMLNPIIYTLRNKDVKGAMKKVCPDPPSSLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8I5</accession>
    <name>A0A452I8I5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I8I5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023970"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027296.1">
        <property type="protein sequence ID" value="ENSGAGP00000023970.1"/>
        <property type="gene ID" value="ENSGAGG00000017529.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF15">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="33"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="597" mass="66846" checksum="BB839FAC1C16A580" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I997</accession>
    <name>A0A452I997_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000024246"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027602.1">
        <property type="protein sequence ID" value="ENSGAGP00000024246.1"/>
        <property type="gene ID" value="ENSGAGG00000017650.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024855">
        <property type="entry name" value="UNC79"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21696">
        <property type="entry name" value="PROTEIN UNC-79 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21696:SF2">
        <property type="entry name" value="PROTEIN UNC-79 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14776">
        <property type="entry name" value="UNC-79"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="861"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1212"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1564"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1637"/>
            <end position="1656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1668"/>
            <end position="1698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1788"/>
            <end position="1852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1887"/>
            <end position="1929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="864"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1221"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1573"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1825"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1918"/>
            <end position="1929"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2643" mass="297728" checksum="E2F12C39AD3782B0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IA47</accession>
    <name>A0A452IA47_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IA47"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024397"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027767.1">
        <property type="protein sequence ID" value="ENSGAGP00000024397.1"/>
        <property type="gene ID" value="ENSGAGG00000017826.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000001">
        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF29">
        <property type="entry name" value="COMPLEMENT C1Q SUBCOMPONENT SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0176">Collagen</keyword>
    <keyword evidence="5" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019545364" description="C1q domain-containing protein" evidence="7">
        <location>
            <begin position="27"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="8">
        <location>
            <begin position="115"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="245" mass="25781" checksum="2AB641785D88AAA3" modified="2019-05-08" version="1">MAKNVWEPVGLGLALLLLTLETTVTGSASHSCYGTPGLPGTPGMPGKDGRDGLKGVKGEPGIPAIPGTRGPKGEKGDPGIPGLPGKNGPMGPPGTRGEPGEYGQPGGPGLPGNYKQKHQSAFSVMRQTGQYPDKNAPVVFNHAITNLSEDYNTTSGKFTCRVPGLYYFVFHTSQTANLCVNMYKNRQKVASFCDHMSNIKQVSSGGLLLRMDAGHQLWLAVNDYNGMVGIAGSDSVFSGFLLFPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IA69</accession>
    <name>A0A452IA69_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IA69"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024585"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027981.1">
        <property type="protein sequence ID" value="ENSGAGP00000024585.1"/>
        <property type="gene ID" value="ENSGAGG00000017951.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000027985.1">
        <property type="protein sequence ID" value="ENSGAGP00000024589.1"/>
        <property type="gene ID" value="ENSGAGG00000017951.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="477"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="538"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="567"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="607"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="638"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="677" mass="77275" checksum="FE151F97CE5C37D5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICC5</accession>
    <name>A0A452ICC5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">High mobility group protein B3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HMGB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ICC5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025434"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028942.1">
        <property type="protein sequence ID" value="ENSGAGP00000025434.1"/>
        <property type="gene ID" value="ENSGAGG00000018592.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21978">
        <property type="entry name" value="HMG-box_HMGB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21979">
        <property type="entry name" value="HMG-box_HMGB_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000013">
        <property type="entry name" value="High mobility group protein B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000017">
        <property type="entry name" value="high mobility group protein B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017967">
        <property type="entry name" value="HMG_boxA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF32">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09011">
        <property type="entry name" value="HMG_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00353">
        <property type="entry name" value="HMG_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0558">Oxidation</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="19">
        <location>
            <begin position="9"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="19">
        <location>
            <begin position="93"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="17">
        <location>
            <begin position="9"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="17">
        <location>
            <begin position="93"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="161"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00073418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="202" mass="22999" checksum="EA02F528354A1C2C" modified="2019-05-08" version="1">MAKGDPKKPKGKMSAYAFFVQTCREEHKKKNPEVPVNFAEFSKKCSERWKTMSGKEKSKFDEMAKADKVRYDREMKDYGPAKGGKKKKDPNAPKRPPSGFFLFCSEFRPKIKSTNPGISIGDVAKKLGEMWNNLSDGEKQPYNNKAAKLKEKYEKDVADYKSKGKFDGAKGAAAKAARKKVEEEDEEDEDDEEEDEEDEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ID96</accession>
    <name>A0A452ID96_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ID96"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025780"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029325.1">
        <property type="protein sequence ID" value="ENSGAGP00000025780.1"/>
        <property type="gene ID" value="ENSGAGG00000018813.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000120">
        <property type="entry name" value="Putative serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF55">
        <property type="entry name" value="SERINE PROTEASE 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="40"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="270" mass="29818" checksum="027286724A25970A" modified="2019-05-08" version="1">MGVCRVCATGWGRDVTSAPVGTAPMEVNEATPTCALGSWIIGGKEVKPHSRPYIASIQLDGRHVCGGFLVWRKWVMTAAHCVIPRHSPAVRIVLGAHSLKHQEASQQIFSIRESIMHPHFNARTVHNDIRMLELNTSAIFNQFVKRIRLPKPNTDLPPGVVCRVLGWGDTSNFGTIPTELRETQTTIVDRKICKALWVGHVNKDMLCAASLNSTLQGVCSGDSGGPLICGGRVHGIVSFSGQRCGNRRYPDIYTRISKYISWVHDVLKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDB5</accession>
    <name>A0A452IDB5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Cytochrome c oxidase subunit 5A, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15 16">Cytochrome c oxidase polypeptide Va</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Mitochondrion inner membrane</location>
            <topology evidence="1 16">Peripheral membrane protein</topology>
            <orientation evidence="1 16">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the cytochrome c oxidase subunit 5A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDB5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025835"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029390.1">
        <property type="protein sequence ID" value="ENSGAGP00000025835.1"/>
        <property type="gene ID" value="ENSGAGG00000018866.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00923">
        <property type="entry name" value="Cyt_c_Oxidase_Va"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.40:FF:000002">
        <property type="entry name" value="cytochrome c oxidase subunit 5A, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.40">
        <property type="entry name" value="Cytochrome c oxidase, subunit Va/VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003204">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036545">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200:SF11">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02284">
        <property type="entry name" value="COX5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48479">
        <property type="entry name" value="Cytochrome c oxidase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 16" id="KW-0349">Heme</keyword>
    <keyword evidence="12 16" id="KW-0408">Iron</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 16" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="141" mass="15922" checksum="FB235B5717F1C3C5" modified="2019-05-08" version="1">PRRFQSKQKTETTAVGARVAVRRGAVLPARCYSHGTQESDEEFDARWVTYFNKPDIDAWELRKGMNTLVGYDLVPEAKISDAALRACRRLNDFATAVCILEVVKDKAGPHKEIYPYVIQELRPTLNELGISTPEELDLDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IDJ1</accession>
    <name>A0A452IDJ1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein P1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">60S acidic ribosomal protein P1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the elongation step of protein synthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer with RPLP2 at the lateral ribosomal stalk of the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IDJ1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025922"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029488.1">
        <property type="protein sequence ID" value="ENSGAGP00000025922.1"/>
        <property type="gene ID" value="ENSGAGG00000018932.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="140" mass="14168" checksum="31AA5FCF514EC9D4" modified="2019-05-08" version="1">GLILHDDEITVMEDKINALIKAAGVNVEPFWPGLFAKALADTDTGSLICNVGAGGAPAAAAPSGGAAPSGGAAPGDRRGCLGKQDLGCGLSSLEGGALGSSPVWRSDLNWPLYCLIVNFCINESPGKEMSLDSTQGVERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFT4</accession>
    <name>A0A452IFT4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IFT4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026753"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030407.1">
        <property type="protein sequence ID" value="ENSGAGP00000026753.1"/>
        <property type="gene ID" value="ENSGAGG00000019490.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF20">
        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019065191" description="TLC domain-containing protein" evidence="7">
        <location>
            <begin position="18"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="8">
        <location>
            <begin position="33"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="259" mass="29882" checksum="74DCC1A6C9C66332" modified="2019-05-08" version="1">MWQTLAIASVFFPGLFALSVRSLGWAAPAWSLKDRILLSSRMVSSVQATMATVSGIIVVLNCKDVVHDRYWLAVEYVWVIVPYMIYDIYIMYFCHWHKSKDTLEKKHSLASVRSFLEKDRLMVTHHLFILVILTPVAQYFRGELGDFFVGCIFVAELSTPFVSLGKILMQLKMQDTLLHKVNGILILVTFFLCRIVLFPFMYWAYARHMGIPIYMVPFHIPMHCNVANASLIAPQLYWFVLICRKAVRLYSSSPSAKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGB8</accession>
    <name>A0A452IGB8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000026955"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030635.1">
        <property type="protein sequence ID" value="ENSGAGP00000026955.1"/>
        <property type="gene ID" value="ENSGAGG00000019621.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030155">
        <property type="term" value="P:regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000041">
        <property type="entry name" value="LIM and calponin homology domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031865">
        <property type="entry name" value="DUF4757"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029978">
        <property type="entry name" value="LMO-7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46767">
        <property type="entry name" value="LIM DOMAIN ONLY PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46767:SF1">
        <property type="entry name" value="LIM DOMAIN ONLY PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15949">
        <property type="entry name" value="DUF4757"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="870"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="1470"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="25"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="568"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1057"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1150"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1269"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1331"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="575"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="620"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1167"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1272"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1290"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1335"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1373"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1417"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1441"/>
            <end position="1462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1540" mass="175484" checksum="3DBA7B3C554128F0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452II09</accession>
    <name>A0A452II09_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A452II09"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027473"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031206.1">
        <property type="protein sequence ID" value="ENSGAGP00000027473.1"/>
        <property type="gene ID" value="ENSGAGG00000019958.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14037">
        <property type="entry name" value="STKc_NAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000072">
        <property type="entry name" value="AP2 associated kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051744">
        <property type="entry name" value="AP2_assoc_SerThr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028182">
        <property type="entry name" value="BMP2K_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47907:SF4">
        <property type="entry name" value="BMP-2-INDUCIBLE PROTEIN KINASE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47907">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15282">
        <property type="entry name" value="BMP2K_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="522"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="574"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="838"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1063"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="465"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="612"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="628"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="672"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="709"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="721"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="743"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="844"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="891"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="957"/>
            <end position="966"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1088" mass="121429" checksum="7DC7EA1F156A5327" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452II74</accession>
    <name>A0A452II74_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ornithine carbamoyltransferase</fullName>
            <ecNumber evidence="5">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Nitrogen metabolism; urea cycle; L-citrulline from L-ornithine and carbamoyl phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452II74"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027552"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031303.1">
        <property type="protein sequence ID" value="ENSGAGP00000027552.1"/>
        <property type="gene ID" value="ENSGAGG00000020004.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00158">
        <property type="reaction ID" value="UER00271"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000009">
        <property type="entry name" value="Ornithine carbamoyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000010">
        <property type="entry name" value="Ornithine carbamoyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001986">
        <property type="entry name" value="PRK00779.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF3">
        <property type="entry name" value="ORNITHINE TRANSCARBAMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0835">Urea cycle</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="14">
        <location>
            <begin position="42"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="13">
        <location>
            <begin position="189"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="356" mass="39880" checksum="910F9AC93956BDCD" modified="2019-05-08" version="1">MPALCGYGCSGQPRVEGVRGLGACFWCRSGQPTQTNVNLKGRDLLTLQNYTAEEIKYLLWTASDLKQRIKDKGEYLPLLQGKSLAMIFEKRSTRTRLSTETGFALLGGHPCFLTTQDIHLGVNESLTDTAWVLSSLTNAVLARVYKQSDLDLLAKEASIPIVNGLSDSYHPIQILADYLTLQEHYGYLKGLTISWIGDGNNVLNSILMSAAKFGMHLRVATPKGFEPDPNIVKVAEEYSKEYSTELFFTSDPLEAASGTNVLVTDTWISMGQEKEKAERMKAFQGYQITMQMGKQAASDWTFLHCLPRKPEEVDDEVFYSPRSLVFQEAENRKWTIMAVMVSLLTDYSPQLQKPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIH4</accession>
    <name>A0A452IIH4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IIH4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027677"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031443.1">
        <property type="protein sequence ID" value="ENSGAGP00000027677.1"/>
        <property type="gene ID" value="ENSGAGG00000020113.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15230">
        <property type="entry name" value="7tmA_OR5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="235"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="321" mass="35858" checksum="6D0847C41CF661BE" modified="2019-05-08" version="1">MAPGNCTKVTGFIFRGITDSPKLQSILFVFFFAIYFCTLVGNVGMIVLIRVDSQLHSPMYFFLSNLSLLDVVYSSTIAPKAMLSSLVTSKDISFPGCVVQFFLFSFCANNELCLLAVMAYDRFVAICNPLLYNVIMSKRICFQLVAGSYLCACINATVHTCSVFSLSFGHSNVLDHFFCDIRPLQEISCSDFRINNRVHFIFAAVTALSTVLIILVSYIYIILNNLRIRSVEGRGKAFSTCVSHLTSVSLLYGTLFFMYLRPTSGNSSDQDRVVSVFYTLVIPMLNPLIYSLRNKEVKHALRKDNSCCLSVFSCPGHSEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJJ7</accession>
    <name>A0A452IJJ7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IJJ7"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031923.1">
        <property type="protein sequence ID" value="ENSGAGP00000028094.1"/>
        <property type="gene ID" value="ENSGAGG00000020414.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="63" mass="6769" checksum="9D7998BD309C3F8D" modified="2025-10-08" version="2">MAGLRSQPQAEQRWLRAARAFVCETLCPAGPGGGGPEQLADLVLRCLRSTWGGRSGELPLGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJZ2</accession>
    <name>A0A452IJZ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Phospholipase A2</fullName>
            <ecNumber evidence="2 7">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The N-terminal C2 domain associates with lipid membranes upon calcium binding.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="2 7"/>
    <dbReference type="AlphaFoldDB" id="A0A452IJZ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028199"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032045.1">
        <property type="protein sequence ID" value="ENSGAGP00000028199.1"/>
        <property type="gene ID" value="ENSGAGG00000020495.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000002">
        <property type="entry name" value="Phospholipase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF32">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 6 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="9">
        <location>
            <begin position="136"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="687" mass="79521" checksum="3F910990C0DE1B2A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKY6</accession>
    <name>A0A452IKY6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IKY6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028492"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032361.1">
        <property type="protein sequence ID" value="ENSGAGP00000028492.1"/>
        <property type="gene ID" value="ENSGAGG00000020646.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000032364.1">
        <property type="protein sequence ID" value="ENSGAGP00000028495.1"/>
        <property type="gene ID" value="ENSGAGG00000020646.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029242">
        <property type="entry name" value="MLANA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15305">
        <property type="entry name" value="MELANOMA ANTIGEN RECOGNIZED BY T-CELLS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15305:SF0">
        <property type="entry name" value="MELANOMA ANTIGEN RECOGNIZED BY T-CELLS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14991">
        <property type="entry name" value="MLANA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="161" mass="18348" checksum="C2871911B6479F76" modified="2019-05-08" version="1">MIHPSSSCDHWRTPGFLRDDIQVFWFQHCLDVYNYWLPTRCDQNQSYKMPKGDHQRDAIFSRGKQYTYLAAEEAAGIGILVVVLAVLLIIGCWYYKRRCGYKNLRSKSSHAGTMRRVAGEDAPLDCKALLQEYSNFNSVVPDAPPAYDKVSADPLPPPYSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IND1</accession>
    <name>A0A452IND1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratricopeptide repeat protein 21A/21B fourth ARM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IND1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029369"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033350.1">
        <property type="protein sequence ID" value="ENSGAGP00000029369.1"/>
        <property type="gene ID" value="ENSGAGG00000021232.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056836">
        <property type="entry name" value="ARM_TT21_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042161">
        <property type="entry name" value="TTC34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44874">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44874:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25068">
        <property type="entry name" value="ARM_TT21_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B fourth ARM" evidence="3">
        <location>
            <begin position="894"/>
            <end position="991"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1065" mass="118625" checksum="369DEDC1C790C22F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452INK6</accession>
    <name>A0A452INK6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Glycoprotein IX platelet</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452INK6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029451"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033441.1">
        <property type="protein sequence ID" value="ENSGAGP00000029451.1"/>
        <property type="gene ID" value="ENSGAGG00000021280.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052313">
        <property type="entry name" value="GPIb-IX-V_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22650">
        <property type="entry name" value="GLYCOPROTEIN IB BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22650:SF6">
        <property type="entry name" value="PLATELET GLYCOPROTEIN IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046922550" description="Glycoprotein IX platelet" evidence="12">
        <location>
            <begin position="31"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="13">
        <location>
            <begin position="32"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="14">
        <location>
            <begin position="99"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24352" checksum="59A004A7152E1C97" modified="2025-10-08" version="2">MAHKMVACPLLWIPATAGFVTLMLFSLVHAEICPLSCTCKPLGEMKGWLVDCSSKGLKEVPDLPVSTRKLYLQNNSMTTVRTGAFDNLHNLEEMNVSDNPWNCDCDILYLKFWLQDFSESSIANVICATPASNERKPLSQLTGNELDNCWKSLPIKCLNFFWRDLALITFAVVVLILTSCALRFSKRLAYQVTTRDYYSAAPLLRKHNLENYMSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPG7</accession>
    <name>A0A452IPG7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IPG7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029792"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033830.1">
        <property type="protein sequence ID" value="ENSGAGP00000029792.1"/>
        <property type="gene ID" value="ENSGAGG00000021513.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050637">
        <property type="entry name" value="NLRP_innate_immun_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45690">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45690:SF19">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="87"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="507" mass="59953" checksum="58BCB205AB038D45" modified="2019-05-08" version="1">MLHAIIIELDYQKYREHIQEEYQVIEDRNARRRHAELMVQQISVTKIRVLFDPNRTGKLYQKKFDYVFYINCRKINCYCRAKPTKEIFEKPEKLLFIIDGFDELNSFLDDEFGLCTDPFEKKPVETVLCSLLRKKVLQKSFLLITARPTALEKLKQNLNIPRYAKIMGFSGDDRKEYFHKFFCNEKKATQAFNFVKENEMLFTMCFVPIVCWIICMVKKEQMERGEDLAQASETTTLVYLLFLNSLLSDHFTDSINLQQSKIILWKLCSLAADGILERKILFEEDDLKRHKLSLSTIHTPFLNKNIFQKGIECESVYSFIHLSFQEVFAAMFYVLEDEETMKKSLSSKKNVTNLLNIYGTSKNNYLMLTVHFLFGLVNKERLSDREKKLNFKTSSEIKLDLLKWFEAEAKNISSLPYKTCEKLHDQLELFHCLYEIQDEEFAKSAMDNFQEIRLEHIRLSTMDEIALLFCVKNGCSKQSLYLCNCMLGIEEREEGWIAKLWKWLFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQ29</accession>
    <name>A0A452IQ29_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vesicle transport protein USE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">USE1-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the USE1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IQ29"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029953"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034012.1">
        <property type="protein sequence ID" value="ENSGAGP00000029953.1"/>
        <property type="gene ID" value="ENSGAGG00000021613.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15860">
        <property type="entry name" value="SNARE_USE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019150">
        <property type="entry name" value="Vesicle_transport_protein_Use1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13050">
        <property type="entry name" value="USE1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13050:SF7">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN USE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09753">
        <property type="entry name" value="Use1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="259" mass="29911" checksum="BD12E7E61E8A4C65" modified="2019-05-08" version="1">MVATRLELNLLRLLSRCEALAAERREPDEWRLEKYVDALEEMLLALKKHSSKPAPELLNEYARKVDFLKGMLEAEKLSSSAEKALANQFLAPGRTPTTAKERTPATKMVHLQTKARYTGKMRSELLGTDPLSVNESEELNLRKRKDFVSEEKQSATELDAVLQHHRSMQEKLAEEMLSLARSLKNNTLAAQNVIKQDNQTLSHSLRMADHNFEKLKDESERLEQHAKKSVNWLLWIMLIVVCFIFISMILFIRIFPKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IR30</accession>
    <name>A0A452IR30_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TSC22 domain family protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TSC-22/Dip/Bun family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IR30"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030556"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034674.1">
        <property type="protein sequence ID" value="ENSGAGP00000030556.1"/>
        <property type="gene ID" value="ENSGAGG00000022003.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21940">
        <property type="entry name" value="ZIP_TSC22D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.490:FF:000002">
        <property type="entry name" value="TSC22 domain family, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000580">
        <property type="entry name" value="TSC22/Bun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047862">
        <property type="entry name" value="TSC22/BUN_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12348">
        <property type="entry name" value="TSC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12348:SF24">
        <property type="entry name" value="TSC22 DOMAIN FAMILY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01166">
        <property type="entry name" value="TSC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58026">
        <property type="entry name" value="Delta-sleep-inducing peptide immunoreactive peptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01289">
        <property type="entry name" value="TSC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="130"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="201" mass="22059" checksum="6F3D9417D5E29B6C" modified="2019-05-08" version="1">MASEECRSPVGLDCCSCCLDLANRSGLEEGAGGENNNLGSPTVSNFRQLRAQLVCENLNADKLSSIMRQDSLEPVVRDPCYLLNQGICNSNIDQTLLSILLFFHSASGASVVAIDNKIEQAMDLVKNHLMYAVREEVEILKEQIKELVEKNSQLERENSLLKTLASPEQLEKFQSRLPADLLSAEEQGRAAPAQHTRGSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITX0</accession>
    <name>A0A452ITX0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GGA protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ITX0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031488"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035712.1">
        <property type="protein sequence ID" value="ENSGAGP00000031488.1"/>
        <property type="gene ID" value="ENSGAGG00000022615.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034394">
        <property type="term" value="P:protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14240">
        <property type="entry name" value="GAT_GGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17008">
        <property type="entry name" value="VHS_GGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1230:FF:000001">
        <property type="entry name" value="ADP-ribosylation factor-binding protein GGA1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000023">
        <property type="entry name" value="ADP-ribosylation factor-binding protein GGA3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000011">
        <property type="entry name" value="ADP-ribosylation factor-binding protein GGA3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044111">
        <property type="entry name" value="GAT_GGA3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027422">
        <property type="entry name" value="GGA1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041198">
        <property type="entry name" value="GGA_N-GAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046996">
        <property type="entry name" value="VHS_GGA3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45905:SF3">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45905">
        <property type="entry name" value="GOLGI-LOCALIZED, GAMMA-ADAPTIN EAR CONTAINING, ARF BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18308">
        <property type="entry name" value="GGA_N-GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="VHS" evidence="12">
        <location>
            <begin position="16"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="14">
        <location>
            <begin position="171"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="GAE" evidence="13">
        <location>
            <begin position="628"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="359"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="201"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="378"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="757" mass="82228" checksum="33B0BFD8C5CF6528" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUQ6</accession>
    <name>A0A452IUQ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cadherin-related family member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Photoreceptor cadherin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Protocadherin-21</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IUQ6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031610"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035848.1">
        <property type="protein sequence ID" value="ENSGAGP00000031610.1"/>
        <property type="gene ID" value="ENSGAGG00000022701.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000111">
        <property type="entry name" value="Cadherin-related family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000113">
        <property type="entry name" value="Cadherin-related family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000122">
        <property type="entry name" value="Cadherin-related family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000124">
        <property type="entry name" value="Cadherin-related family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000126">
        <property type="entry name" value="Cadherin-related family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000177">
        <property type="entry name" value="Cadherin-related family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF413">
        <property type="entry name" value="CADHERIN RELATED FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0732">Signal</keyword>
    <keyword evidence="2 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019329350" description="Cadherin-related family member 1" evidence="15">
        <location>
            <begin position="22"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="703"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="45"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="138"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="250"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="362"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="476"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="581"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="865" mass="95806" checksum="FDC273D12889B347" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWH4</accession>
    <name>A0A452IWH4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IWH4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036829.1">
        <property type="protein sequence ID" value="ENSGAGP00000032482.1"/>
        <property type="gene ID" value="ENSGAGG00000023235.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="354" mass="36401" checksum="B98A39728F32BAC6" modified="2019-05-08" version="1">MLPALDLDGELILKVSTGAPGWDSKRQGDGQETLTEQTVSPAQPSTGPGSLPLSEAGGFSLETTAPPVTSADKDGVAIHPSFSTPRAVELDESIPPDPSAATTKGTRLQTPAPAISADATTGLRNKNQSANSSSELPTPALAANTTDANQPGQNGTEPSSLTGPTGTATVAPTHPPPQDALATTSSAQLELSAFNTLGPTTGNPEPTPQTAQENATEESIAGPTTVPAVTTTGSTPAATTPSTATATLRVSSPNRTATTAARQPSADPVHEKASVLDVGDDDGQDLPSSAVALRMGADPLVIGVISMFVVMVGILALVGFLRYRQRHSRMEFRRLQDLPMDDMMEDTPLSLYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWM2</accession>
    <name>A0A452IWM2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin-dependent peroxide reductase, mitochondrial</fullName>
            <ecNumber evidence="2">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Thioredoxin-dependent peroxiredoxin 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. Required for the maintenance of physical strength.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer; disulfide-linked, upon oxidation. 6 homodimers assemble to form a ring-like dodecamer. Interacts with NEK6. Interacts with LRRK2. Interacts with MAP3K13. Interacts with RPS6KC1 (via PX domain).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452IWM2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032309"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036628.1">
        <property type="protein sequence ID" value="ENSGAGP00000032309.1"/>
        <property type="gene ID" value="ENSGAGG00000023123.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03015">
        <property type="entry name" value="PRX_Typ2cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000003">
        <property type="entry name" value="Peroxiredoxin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF128">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="13">
        <location>
            <begin position="68"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="261" mass="28437" checksum="7A967775D90060A1" modified="2019-05-08" version="1">MAAALRRLLRPSVARATPLSWGACAAALRPPVPAAAAAAAGGGRKCTAQAELALPRLHFSTSSSRFVPAVTQHAPYFKGTAVVNGEFKEMSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFRDVNCEVVAVSVDSHFCHLAWTKTPRKSGGLGHMNIPLLSDLTKQISRDYGVLLEGPGLALRGLFIIDPNGIIKHLSINDLPVGRSVEETLRLVKAFQYVDTHGEVCPANWTPESPTIKPSPEASKEYFEKVHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWZ9</accession>
    <name>A0A452IWZ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytoplasmic aconitate hydratase</fullName>
            <ecNumber evidence="4">4.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Citrate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Iron-responsive element-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452IWZ9"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036968.1">
        <property type="protein sequence ID" value="ENSGAGP00000032616.1"/>
        <property type="gene ID" value="ENSGAGG00000023315.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030350">
        <property type="term" value="F:iron-responsive element binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01586">
        <property type="entry name" value="AcnA_IRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01580">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000005">
        <property type="entry name" value="cytoplasmic aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000005">
        <property type="entry name" value="Iron-responsive element-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044137">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006757">
        <property type="entry name" value="PRK09277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009520">
        <property type="entry name" value="PRK12881.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 18" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 18" id="KW-0408">Iron</keyword>
    <keyword evidence="11 18" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="19">
        <location>
            <begin position="66"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="20">
        <location>
            <begin position="693"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="889" mass="98035" checksum="0562FDC8BC858358" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J043</accession>
    <name>A0A452J043_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J043"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033719"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038193.1">
        <property type="protein sequence ID" value="ENSGAGP00000033717.1"/>
        <property type="gene ID" value="ENSGAGG00000024002.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000038197.1">
        <property type="protein sequence ID" value="ENSGAGP00000033719.1"/>
        <property type="gene ID" value="ENSGAGG00000024002.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000038200.1">
        <property type="protein sequence ID" value="ENSGAGP00000033722.1"/>
        <property type="gene ID" value="ENSGAGG00000024002.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="406" mass="46195" checksum="8149695C1A6016F3" modified="2019-05-08" version="1">MAENGTDCDQRRIGMIKEQHNGNFTDPSLPCERKRRDQEERQNIVLWRQPLITLQYFFLETLINLKEWTIKLWHRRSIVVSLLLLLAVLTAAYYIEGTHQQYVQYTEKKFLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSVNFPEPPYPDQIICPDEEGIEGSISLWTIISKVRLEACMWGAGTAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAEAAQDFASRAKLAVQNLVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKLFVIITFSKHIVEQMVSLIGAVPSIGPSLQKPFQEYLEAQRIKLHHKSDNGVPQGENWLSWMFEKLVIVMVCYFILSIINSMAQSYAKRVQQKMYSEEKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J076</accession>
    <name>A0A452J076_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AGR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J076"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033754"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038234.1">
        <property type="protein sequence ID" value="ENSGAGP00000033754.1"/>
        <property type="gene ID" value="ENSGAGG00000024026.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000038235.1">
        <property type="protein sequence ID" value="ENSGAGP00000033755.1"/>
        <property type="gene ID" value="ENSGAGG00000024026.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002162">
        <property type="term" value="F:dystroglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02960">
        <property type="entry name" value="AGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000036">
        <property type="entry name" value="anterior gradient protein 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051099">
        <property type="entry name" value="AGR/TXD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15337:SF5">
        <property type="entry name" value="ANTERIOR GRADIENT PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15337">
        <property type="entry name" value="ANTERIOR GRADIENT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13899">
        <property type="entry name" value="Thioredoxin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604611" evidence="3">
        <location>
            <begin position="23"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="165" mass="19064" checksum="1C69F3CEB94C6351" modified="2019-05-08" version="1">MMHSALALSLLLIAVSSNLAMAIKKEKRAPQTLSRGWGDDITWVQTYEEGLFQARKSNKPLMVIHHLEDCQYCQALKKVFAENEEIQEMAQNNFIMLNLMHETTDKNLSPDGQYVPRIMFVDPSLTVRADITGRYSNRLYTYEPQDIPFLIENMKKALRLIQTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1B9</accession>
    <name>A0A452J1B9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RNA helicase Mov10l1</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the primary piRNA metabolic process. Specifically binds to piRNA precursors and promotes the generation of intermediate piRNA processing fragments that are subsequently loaded to Piwi proteins. Acts via its ATP-dependent RNA helicase activity: displays 5'-3' RNA unwinding activity and probably mediates unwinding and funneling of single-stranded piRNA precursor transcripts to the endonuclease that catalyzes the first cleavage step of piRNA processing to generate piRNA intermediate fragments that are subsequently loaded to Piwi proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with PIWIL1. Interacts with PIWIL2. Interacts with PIWIL4. Interacts with HSPA2. Interacts with PLD6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family. SDE3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452J1B9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034112"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038627.1">
        <property type="protein sequence ID" value="ENSGAGP00000034112.1"/>
        <property type="gene ID" value="ENSGAGG00000024226.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18078">
        <property type="entry name" value="DEXXQc_Mov10L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000999">
        <property type="entry name" value="Mov10 like RISC complex RNA helicase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000864">
        <property type="entry name" value="Mov10-like RISC complex RNA helicase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049080">
        <property type="entry name" value="MOV-10-like_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45418">
        <property type="entry name" value="CANCER/TESTIS ANTIGEN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45418:SF1">
        <property type="entry name" value="CANCER_TESTIS ANTIGEN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21634">
        <property type="entry name" value="MOV-10_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase MOV-10-like beta-barrel" evidence="16">
        <location>
            <begin position="553"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="14">
        <location>
            <begin position="714"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="14">
        <location>
            <begin position="825"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="15">
        <location>
            <begin position="913"/>
            <end position="1118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1154" mass="129824" checksum="87922D6EFA748719" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1M8</accession>
    <name>A0A452J1M8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Solute carrier family 2, facilitated glucose transporter member 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Glucose transporter type 10</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Facilitative glucose transporter required for the development of the cardiovascular system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose(out) = D-glucose(in)</text>
            <dbReference type="Rhea" id="RHEA:60376"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J1M8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034289"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038828.1">
        <property type="protein sequence ID" value="ENSGAGP00000034289.1"/>
        <property type="gene ID" value="ENSGAGG00000024367.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000038831.1">
        <property type="protein sequence ID" value="ENSGAGP00000034292.1"/>
        <property type="gene ID" value="ENSGAGG00000024367.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072359">
        <property type="term" value="P:circulatory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904659">
        <property type="term" value="P:D-glucose transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17436">
        <property type="entry name" value="MFS_GLUT10_Class3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000790">
        <property type="entry name" value="Solute carrier family 2 member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000164">
        <property type="entry name" value="solute carrier family 2, facilitated glucose transporter member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050820">
        <property type="entry name" value="MFS_Sugar_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48023">
        <property type="entry name" value="D-XYLOSE-PROTON SYMPORTER-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48023:SF7">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="440"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="512"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="17">
        <location>
            <begin position="11"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="405"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="580" mass="63536" checksum="96BCD6436281243F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1R8</accession>
    <name>A0A452J1R8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">LIM and cysteine-rich domains protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter. Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J1R8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034329"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038871.1">
        <property type="protein sequence ID" value="ENSGAGP00000034329.1"/>
        <property type="gene ID" value="ENSGAGG00000024397.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070886">
        <property type="term" value="P:positive regulation of calcineurin-NFAT signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010611">
        <property type="term" value="P:regulation of cardiac muscle hypertrophy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09340">
        <property type="entry name" value="LIM1_Testin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09829">
        <property type="entry name" value="PET_testin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000079">
        <property type="entry name" value="LIM and cysteine-rich domains protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000005">
        <property type="entry name" value="Testin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010442">
        <property type="entry name" value="PET_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033724">
        <property type="entry name" value="PET_testin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047120">
        <property type="entry name" value="Pk/Esn/Tes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211:SF0">
        <property type="entry name" value="LIM AND CYSTEINE-RICH DOMAINS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06297">
        <property type="entry name" value="PET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51303">
        <property type="entry name" value="PET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 15" id="KW-0440">LIM domain</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PET" evidence="18">
        <location>
            <begin position="94"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="17">
        <location>
            <begin position="236"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="17">
        <location>
            <begin position="302"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="360" mass="40284" checksum="0B996153F5B17EBC" modified="2019-05-08" version="1">MDLSPGIQKMSVSQQQSGRGVPCLRCRGMCTGFEPHSWRKVCKSCKCSQEDHSLSSDAEDDRKIGRLLSDSKYSTLTARVKGGDGVRIYKRNRMIITNPIVSRKDPTFDTITYEWAPPGLTQKLAMQYMELIPKDMQPVAGTEGASYRRRQLMRQLPIYDQDSSHCRGLAESEIKLMEDFVKKYKSDALGVGEVALPGQGGSAKEEGKQHDKNVAASKPPVSANGALSDAPTGMEYFCDNCKQVVATDCPVVYVDRAGYSRQWHPGCFVCYKCSEPLVDLIYFWKNGAVWCGRHYCESIRPRCAACDEIIFSEDYQQAEGLTWHKKHFTCLVCETLLTGKSFSLDKAKLLCTTCSKTKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J2L9</accession>
    <name>A0A452J2L9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J2L9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034561"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039133.1">
        <property type="protein sequence ID" value="ENSGAGP00000034561.1"/>
        <property type="gene ID" value="ENSGAGG00000024570.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15230">
        <property type="entry name" value="7tmA_OR5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="29"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="297" mass="33872" checksum="975FD1EF45348D11" modified="2019-05-08" version="1">MLQYKNLEYMVHAILFGMFLFIYTMSLVGNLSMISLIRIETRLHTPIYFFLSNLSFGDITYSSVIAPKALVNFLAERKVICFAECVTQFFFQAFCVNSEALLLAVMAYYRFIAICKSLMYTRIMSRKVCVQLVAASYIYDSINAIVRAGSLFSLSFCGPNIIDHFFCDDLPPIQKLFCSDTHMGDTMHFLLAVIVALSTILVILVSYVSSMVAILRIQSNQGSRKAFSTYASHLMAYGTLFFMYLRPTSSNSSEYDKVVAIFYSFVIPMLNPLIYSLRNKEVKDALRKTIYQKIIPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4D6</accession>
    <name>A0A452J4D6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">E3 ubiquitin-protein ligase RNF216</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">RING finger protein 216</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">RING-type E3 ubiquitin transferase RNF216</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Triad domain-containing protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Ubiquitin-conjugating enzyme 7-interacting protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts with UBE2L3 and to some extent with UBE2L6. Interacts with TRAF3, TLR3, TLR4, TLR5 and TLR9. Isoform 3/ZIN binds RIPK1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452J4D6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035296"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039965.1">
        <property type="protein sequence ID" value="ENSGAGP00000035296.1"/>
        <property type="gene ID" value="ENSGAGG00000025087.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20339">
        <property type="entry name" value="BRcat_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20353">
        <property type="entry name" value="Rcat_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16630">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000016">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF216 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000249">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF216 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047545">
        <property type="entry name" value="BRcat_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047546">
        <property type="entry name" value="Rcat_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047544">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058758">
        <property type="entry name" value="UBA_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF47">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26200">
        <property type="entry name" value="Rcat_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26191">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26112">
        <property type="entry name" value="UBA_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="25">
        <location>
            <begin position="578"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="127"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="277"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="818"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="818"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00067770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00075675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00080639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="933" mass="105808" checksum="A7048066BD793307" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4K1</accession>
    <name>A0A452J4K1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J4K1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035404"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040084.1">
        <property type="protein sequence ID" value="ENSGAGP00000035404.1"/>
        <property type="gene ID" value="ENSGAGG00000025168.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038166">
        <property type="term" value="P:angiotensin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008217">
        <property type="term" value="P:regulation of blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009436">
        <property type="entry name" value="AGTRAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16521">
        <property type="entry name" value="TYPE-1 ANGIOTENSIN II RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16521:SF3">
        <property type="entry name" value="TYPE-1 ANGIOTENSIN II RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06396">
        <property type="entry name" value="AGTRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00805">
        <property type="entry name" value="AGTRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="158" mass="17819" checksum="9A9047F479DA759C" modified="2019-05-08" version="1">MEVPAVNLKAIVLVHWLLTTWGCLEFSDSYTWGNFTVLAVGVWAIAQRDSVDAIIMFLIGLLITIVLDIINISLFYPRHSSLSDLERFSSGMAIFSLLLKPLSCLFVYQMYRERGGEYIVNLGFLSVGRDRSSYQSIDQHDAPCPYPDLDSKPAPHPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J620</accession>
    <name>A0A452J620_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">chitinase</fullName>
            <ecNumber evidence="4">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452J620"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035934"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040676.1">
        <property type="protein sequence ID" value="ENSGAGP00000035934.1"/>
        <property type="gene ID" value="ENSGAGG00000025485.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02872">
        <property type="entry name" value="GH18_chitolectin_chitotriosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.140.10:FF:000001">
        <property type="entry name" value="Acidic mammalian chitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000081">
        <property type="entry name" value="Chitinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000001">
        <property type="entry name" value="Chitinase 3-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF188">
        <property type="entry name" value="ACIDIC MAMMALIAN CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="6" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GH18" evidence="16">
        <location>
            <begin position="17"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="15">
        <location>
            <begin position="422"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="471" mass="50989" checksum="785915A70877D9A5" modified="2019-05-08" version="1">MANSPMTSPPCSSGSAYLLTCYFTNWGQYRPGLGKFKPDNIDPCLCTHLIYALAGMSNNQITTIEWNDVTLYKSFNGLNIFTCFETLFFKPSLCTNGQLKTLLAIGGWNFGTALFSAMVSTPQNCQTFINSVITFLRQYEFDGLDFDWEYPGSRGTGQGSLHHPGQEMLEAFEQEAKQINKPRLLVTAAVAAGLSNIESGYQVPQLEGYTGENSPLYKGTADTGSYVYFNVDYALNYWKNNGAPAEKLIVGFPTYRHTFILSNPSNTAVGASTSGTGPAGPYTRQSGFWAYYEICTFLKNGATQAWDTPQDVPYAYKGNEWLGYDNIKSFNIKAQWLMKNNFGGAMVWSLDLDDFTGTFCNQVKYPLINTLKTALGLQSTSCTAPAQPISPITEAPGNGSGSGSGSFGSNPSSGSGSSSGGTGFCASKANGLYPDPSNSNSFYSCVNGRTYLENCQAGLVFDSSCSCCNWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J691</accession>
    <name>A0A452J691_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rho-related GTP-binding protein RhoG</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in immunological synaptic F-actin density and architecture organization. Regulates actin reorganization in lymphocytes, possibly through the modulation of Rac1 activity. Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. Binds phospholipids in an activation-dependent manner; thereby acting as an anchor for other proteins to the plasma membrane (PM). Plays a role in exocytosis of cytotoxic granules (CG) by lymphocytes/Component of the exocytosis machinery in natural killer (NK) and CD8+ T cells. Promotes the docking of cytotoxic granules (CG) to the plasma membrane through the interaction with UNC13D. Involved in the cytotoxic activity of lymphocytes/primary CD8+ T cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with ARHGEF26. Interacts with ARHGEF16. Interacts with UNC13D; the interaction increases RhoG affinity to the membrane lipids, targets UNC13D to membrane lipids and facilitates cytotoxic granule (CG) docking to the plasma membrane.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J691"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035862"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040600.1">
        <property type="protein sequence ID" value="ENSGAGP00000035862.1"/>
        <property type="gene ID" value="ENSGAGG00000025444.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000118">
        <property type="entry name" value="Rho-related GTP-binding protein RhoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00069400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="191" mass="21437" checksum="1E088A2E3EEFDBAF" modified="2019-05-08" version="1">MQSIKCVVVGDGAVGKTCLLICYTTNAFPKEYIPTVFDNYSAQNTVDGRTINLNLWDTAGQEEYDRLRTLSYPQTNVFIICFSIASPPSYENVKHKWYPEVCHHCPNVPILLVGTKKDLRNNPDAIKKLKEQNQLPVTTQQGVNLSKQIHAVKYMECSALNQEGIKEVFTEAVRAVLNPAPVKPKKPCILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J6B4</accession>
    <name>A0A452J6B4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NIBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J6B4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035887"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040627.1">
        <property type="protein sequence ID" value="ENSGAGP00000035887.1"/>
        <property type="gene ID" value="ENSGAGG00000025459.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058565">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058564">
        <property type="entry name" value="TPR_TRAPPC9_Trs120"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013935">
        <property type="entry name" value="Trs120_TRAPPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058563">
        <property type="entry name" value="Trs120_TRAPPC9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512:SF5">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26254">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26280">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26251">
        <property type="entry name" value="TPR_TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08626">
        <property type="entry name" value="TRAPPC9-Trs120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="4">
        <location>
            <begin position="186"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 TPR region" evidence="5">
        <location>
            <begin position="362"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 first Ig-like" evidence="6">
        <location>
            <begin position="540"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="989" mass="110842" checksum="89D47D20ED7D3D4B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J771</accession>
    <name>A0A452J771_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphorylase b kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Glycan biosynthesis; glycogen metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Lipid-anchor</topology>
            <orientation evidence="2 14">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="13">Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the phosphorylase b kinase regulatory chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J771"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036219"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041000.1">
        <property type="protein sequence ID" value="ENSGAGP00000036219.1"/>
        <property type="gene ID" value="ENSGAGG00000025657.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00163"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000004">
        <property type="entry name" value="Phosphorylase b kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045583">
        <property type="entry name" value="KPBA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008734">
        <property type="entry name" value="PHK_A/B_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749:SF4">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT ALPHA, SKELETAL MUSCLE ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19292">
        <property type="entry name" value="KPBB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="10 14" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 14" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="11 13 14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 13 14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="15">
        <location>
            <begin position="8"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphorylase b kinase regulatory subunit alpha/beta C-terminal" evidence="16">
        <location>
            <begin position="1059"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-farnesyl cysteine" evidence="13">
        <location>
            <position position="1234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR608734-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF19292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1237" mass="138562" checksum="F0F69E4276FF5710" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J781</accession>
    <name>A0A452J781_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452J781"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036229"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041011.1">
        <property type="protein sequence ID" value="ENSGAGP00000036229.1"/>
        <property type="gene ID" value="ENSGAGG00000025664.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051438">
        <property type="entry name" value="RNF_E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034734">
        <property type="entry name" value="ZF_C2HC_RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46016">
        <property type="entry name" value="ZINC FINGER, RING/FYVE/PHD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18574">
        <property type="entry name" value="zf_C2HC_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51803">
        <property type="entry name" value="ZF_C2HC_RNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019372860" description="RING-type E3 ubiquitin transferase" evidence="6">
        <location>
            <begin position="18"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC RNF-type" evidence="7">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="192" mass="22508" checksum="65FD8EB4AC251132" modified="2019-05-08" version="1">MESVIFVLISLCRFCRSCIVTSVRNNTWTCPYCRAYLPSEGIPATDIAKKMKGLYQNCTECDTQECLSEMRVHLRTCEQYIEKYGPLQELGDTVTRYACPFCQCELHEDDLMDHCLTYHRTERRAVYCPICRLIPGGDPSYFSRNFIRHLQLRHTFYHEDYIDINIVEEVLTENVLHPSFLEYAQVNHPNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7B2</accession>
    <name>A0A452J7B2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J7B2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036404"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041228.1">
        <property type="protein sequence ID" value="ENSGAGP00000036404.1"/>
        <property type="gene ID" value="ENSGAGG00000025813.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF222">
        <property type="entry name" value="V-SET AND IMMUNOGLOBULIN DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019513359" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="26"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="423"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="21"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="134"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="323"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="528" mass="57262" checksum="86F00230E25C893F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7M4</accession>
    <name>A0A452J7M4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J7M4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036512"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041350.1">
        <property type="protein sequence ID" value="ENSGAGP00000036512.1"/>
        <property type="gene ID" value="ENSGAGG00000025892.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004487">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF15">
        <property type="entry name" value="BIFUNCTIONAL METHYLENETETRAHYDROFOLATE DEHYDROGENASE_CYCLOHYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="1">
        <location>
            <begin position="38"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="117" mass="12746" checksum="273799EE84DE1352" modified="2019-05-08" version="1">MTGLVHPLHSCLGVGGGQGETRPTSRCLPLLQNDAVIISGRKLAQQIRQEARHEVEQWVAAGNKRPHLSVVLVGENPASHSYVLNKTKAAADVGRFLHSCLCALTNRTLNNEYLELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFZ0</accession>
    <name>A0A452GFZ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">May act as a GTPase-activating protein for Rab family protein(s).</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GFZ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000416"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000468.1">
        <property type="protein sequence ID" value="ENSGAGP00000000416.1"/>
        <property type="gene ID" value="ENSGAGG00000000364.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071889">
        <property type="term" value="F:14-3-3 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000009">
        <property type="entry name" value="TBC1 domain family member 22A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000001">
        <property type="entry name" value="TBC1 domain family member 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000004">
        <property type="entry name" value="TBC1 domain family, member 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF462">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="4">
        <location>
            <begin position="208"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="503" mass="58702" checksum="9B55856FCA62D027" modified="2019-05-08" version="1">MAAESSKQFWKRSAKLPGSIQPVYGAQHPPLDPRLTKNFIKERSKGNALPLKNKKASSFHEFARNTSDAWDIGDDEDEDFSSFQTLNSKVAKATAAQVLENHSKLRVKPERAQSALSEMPTNCKVIKSSSEAQLARSPEDACVRNALHKQQSLPLRPIIPLVARISDQNASGAPPMTVREKTRLEKFRQLLSSHNTDLDELRKCSWPGVPREVRPVTWRLLSGYLPANKERRKLTLQRKREEYFGFIEQYYDSRNEEHHQDTYRQIHIDIPRTNPLIPLFQQPLVQEIFERILFIWAIRHPASGYVQGINDLVTPFFVVFLSEHVEEDVENFDVTTLSQDVLRSIEADSFWCMSKLLDGIQDNYTFAQPGIQKKVKALEELVSRIDEQVHNHFRKYEVEYLQFAFRWMNNLLMRELPLRCTIRLWDTYQSEQEGFSHFHLYVCAAFLIKWRKEILDEEDFQGLLMLLQNLPTIHWGNEEIGLLLAEAYRLKYMFADAPNHYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGR4</accession>
    <name>A0A452GGR4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Vitellogenin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GGR4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000754"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000851.1">
        <property type="protein sequence ID" value="ENSGAGP00000000754.1"/>
        <property type="gene ID" value="ENSGAGG00000000594.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034362">
        <property type="term" value="C:low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034359">
        <property type="term" value="C:mature chylomicron"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050750">
        <property type="term" value="F:low-density lipoprotein particle receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030301">
        <property type="term" value="P:cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006642">
        <property type="term" value="P:triglyceride mobilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.230.10:FF:000003">
        <property type="entry name" value="Apolipoprotein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.230.10">
        <property type="entry name" value="Lipovitellin, beta-sheet shell regions, chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.20">
        <property type="entry name" value="Vitellinogen, superhelical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052418">
        <property type="entry name" value="Apolipoprotein_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015819">
        <property type="entry name" value="Lipid_transp_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011030">
        <property type="entry name" value="Lipovitellin_superhlx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015816">
        <property type="entry name" value="Vitellinogen_b-sht_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015255">
        <property type="entry name" value="Vitellinogen_open_b-sht"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001747">
        <property type="entry name" value="Vitellogenin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13769">
        <property type="entry name" value="APOLIPOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13769:SF1">
        <property type="entry name" value="APOLIPOPROTEIN B-100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09172">
        <property type="entry name" value="Vit_open_b-sht"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01347">
        <property type="entry name" value="Vitellogenin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01169">
        <property type="entry name" value="DUF1943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00638">
        <property type="entry name" value="LPD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56968">
        <property type="entry name" value="Lipovitellin-phosvitin complex, beta-sheet shell regions"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48431">
        <property type="entry name" value="Lipovitellin-phosvitin complex, superhelical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51211">
        <property type="entry name" value="VITELLOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="6" id="KW-0162">Chylomicron</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="11" id="KW-0427">LDL</keyword>
    <keyword evidence="10" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="12 19" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="15" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="17" id="KW-0850">VLDL</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019431049" description="Vitellogenin domain-containing protein" evidence="19">
        <location>
            <begin position="18"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="Vitellogenin" evidence="20">
        <location>
            <begin position="23"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="163"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="795" mass="89276" checksum="7733C7EAFB5FBDBD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGW1</accession>
    <name>A0A452GGW1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GGW1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000817"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000920.1">
        <property type="protein sequence ID" value="ENSGAGP00000000817.1"/>
        <property type="gene ID" value="ENSGAGG00000000606.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026642">
        <property type="entry name" value="Glcci1/FAM117"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14972">
        <property type="entry name" value="AGAP011572-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14972:SF7">
        <property type="entry name" value="PROTEIN FAM117A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15388">
        <property type="entry name" value="FAM117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="226"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="226"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="434" mass="46909" checksum="856AAD7F8B3DDEB7" modified="2019-05-08" version="1">LRGGSGKRWRGVGGGRSGLEQSGGGEVDVPCATAVEKTCRTRAPRVRRTSSLDTILGSYLLGQWPRDADGAFASCMNDKATQTPTSWHEVEVGKASASTHKRSASWGSTDHRREIAKLKQQLQRTKLSGKEKDRSSPLQGDHAVLGSVRDSPPGLPPASPVLRLSPCLHRSLEGLNQELEEVFVKEQGDEELLRILDVPDGHRAPAPPQRGSEDLLLLTLEPSSSSCSSLSLSPSPSVPLRSSPHTPARAATEELSSALEEPLPDPKEKETSQKSLPSLLEDGSPSPVLAFASSPRPNHSYMFKREPPEGCEKVRAFEEALSPSPDQNFLPSCPDKNKVHFNPTGSAFCPVSLVKPLFPNMGFLFRSFPTTPSPVPPGTFTSCQSTAPGPFLGSRKESEADGFSEVSKSPPLNFEHWKRTQTEESVLFHSSLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGW3</accession>
    <name>A0A452GGW3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000000807"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000909.1">
        <property type="protein sequence ID" value="ENSGAGP00000000807.1"/>
        <property type="gene ID" value="ENSGAGG00000000675.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19870">
        <property type="entry name" value="DSRM_SON-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000053">
        <property type="entry name" value="protein SON isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032922">
        <property type="entry name" value="SON"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46528">
        <property type="entry name" value="PROTEIN SON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46528:SF1">
        <property type="entry name" value="PROTEIN SON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14709">
        <property type="entry name" value="DND1_DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="G-patch" evidence="4">
        <location>
            <begin position="4376"/>
            <end position="4422"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="4441"/>
            <end position="4511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="31"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="96"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1171"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2385"/>
            <end position="4125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2385"/>
            <end position="2403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="2404"/>
            <end position="2441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2456"/>
            <end position="2473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2481"/>
            <end position="2503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2511"/>
            <end position="2530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="2531"/>
            <end position="2540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2541"/>
            <end position="2563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="2578"/>
            <end position="2600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2601"/>
            <end position="2623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2631"/>
            <end position="2655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2663"/>
            <end position="2686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2695"/>
            <end position="2719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2727"/>
            <end position="2751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2759"/>
            <end position="2781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2789"/>
            <end position="2811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2819"/>
            <end position="2841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2849"/>
            <end position="2871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2879"/>
            <end position="2903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2911"/>
            <end position="2928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="2936"/>
            <end position="2952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2973"/>
            <end position="2986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3003"/>
            <end position="3016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3033"/>
            <end position="3054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3063"/>
            <end position="3076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="3082"/>
            <end position="3093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3094"/>
            <end position="3126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3134"/>
            <end position="3144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3152"/>
            <end position="3164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="3173"/>
            <end position="3182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3183"/>
            <end position="3195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="3205"/>
            <end position="3221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="3235"/>
            <end position="3250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="3265"/>
            <end position="3274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3305"/>
            <end position="3324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3334"/>
            <end position="3347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="3384"/>
            <end position="3394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3395"/>
            <end position="3404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3419"/>
            <end position="3474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="3477"/>
            <end position="3491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3512"/>
            <end position="3534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3542"/>
            <end position="3559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="3567"/>
            <end position="3581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3602"/>
            <end position="3624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="3627"/>
            <end position="3640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="3684"/>
            <end position="3694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3695"/>
            <end position="3704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3719"/>
            <end position="3744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3752"/>
            <end position="3769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="3777"/>
            <end position="3791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3812"/>
            <end position="3834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3842"/>
            <end position="3859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="3867"/>
            <end position="3881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3882"/>
            <end position="3894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3902"/>
            <end position="3922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="3925"/>
            <end position="3941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3962"/>
            <end position="3977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="3978"/>
            <end position="4048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="4056"/>
            <end position="4100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="4103"/>
            <end position="4125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="4529" mass="502126" checksum="91AE98C7538A2091" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GI73</accession>
    <name>A0A452GI73_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphatase and actin regulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">Binds PPP1CA and actin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the phosphatase and actin regulator family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GI73"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001347"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001511.1">
        <property type="protein sequence ID" value="ENSGAGP00000001347.1"/>
        <property type="gene ID" value="ENSGAGG00000001084.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004018">
        <property type="entry name" value="RPEL_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751:SF7">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR PHACTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02755">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00707">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51073">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="401"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="439"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="477"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="123"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="212"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="450"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="292"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="334"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU301113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="559" mass="62889" checksum="1FD9116180E08A21" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIL2</accession>
    <name>A0A452GIL2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Armadillo repeat-containing protein 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GIL2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001493"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001703.1">
        <property type="protein sequence ID" value="ENSGAGP00000001493.1"/>
        <property type="gene ID" value="ENSGAGG00000001203.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000489">
        <property type="entry name" value="Armadillo repeat containing 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000061">
        <property type="entry name" value="armadillo repeat-containing protein 8 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000070">
        <property type="entry name" value="armadillo repeat-containing protein 8 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038739">
        <property type="entry name" value="ARMC8/Vid28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15651">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15651:SF7">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="7">
        <location>
            <begin position="61"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="693" mass="77610" checksum="721078659F9F88B0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKV7</accession>
    <name>A0A452GKV7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3K p85 subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GKV7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002241"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002565.1">
        <property type="protein sequence ID" value="ENSGAGP00000002241.1"/>
        <property type="gene ID" value="ENSGAGG00000001294.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12926">
        <property type="entry name" value="iSH2_PIK3R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09930">
        <property type="entry name" value="SH2_cSH2_p85_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09942">
        <property type="entry name" value="SH2_nSH2_p85_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000006">
        <property type="entry name" value="Phosphatidylinositol 3-kinase regulatory subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000014">
        <property type="entry name" value="Phosphatidylinositol 3-kinase regulatory subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1490:FF:000001">
        <property type="entry name" value="Putative phosphatidylinositol 3-kinase regulatory subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032498">
        <property type="entry name" value="PI3K_P85_iSH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035020">
        <property type="entry name" value="PI3kinase_P85_cSH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035022">
        <property type="entry name" value="PI3kinase_P85_nSH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16454">
        <property type="entry name" value="PI3K_P85_iSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00678">
        <property type="entry name" value="PI3KINASEP85"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="7">
        <location>
            <begin position="82"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="7">
        <location>
            <begin position="374"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="475" mass="54677" checksum="3B2CB74D0E43ED42" modified="2019-05-08" version="1">MPRTNAGWCLFGVVPGQPESRACGTVPGLVASRSWPEQGPSAAQLQCSPGFLAALPPKPSKPKPGGASLANGSSAPLQEAEWYWGDISREEVNEKLRDTPDGTFLVRDASSKVQGEYTLTLRKGGNNKLIKIFHREGKYGFSEPLTFSSVVELIHHYRHESLAQYNAKLDSKLLYPISKDQQDQLVKEDSVEAVGEQLKVYHQQYQDKSREYDLLYEEYTRTSQELQMKRTAIEAFNETIKIFEEQCQTQEKCSREYIERFRREGNEKEVQRILMNSEKLKSRVTEIHDSKMKLEQDLKKQTSENREIDKRMNSLKPDLMQLRKLRDQYLVWLTQKGARQKKINEWLGIKNETEDQYSMMDDDEELPHHEERTWYVGKINRVQAEEMLCGKRDGTFLIRESSQKGCYACSVVVDGDTKHCVIYKTVTGYGFAEPYNLYASLKDLVLHYKHMSLVQHNDSLNVTLAYPVLSQPPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GN01</accession>
    <name>A0A452GN01_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribonuclease H</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452GN01"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003228"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003724.1">
        <property type="protein sequence ID" value="ENSGAGP00000003228.1"/>
        <property type="gene ID" value="ENSGAGG00000002613.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="11">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="10">
        <location>
            <begin position="314"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="707"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="734" mass="83093" checksum="4D74DBEA0C72DA96" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GN33</accession>
    <name>A0A452GN33_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Formylglycine-generating enzyme</fullName>
            <ecNumber evidence="15">1.8.3.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">C-alpha-formylglycine-generating enzyme 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Sulfatase-modifying factor 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Oxidase that catalyzes the conversion of cysteine to 3-oxoalanine on target proteins, using molecular oxygen and an unidentified reducing agent. 3-oxoalanine modification, which is also named formylglycine (fGly), occurs in the maturation of arylsulfatases and some alkaline phosphatases that use the hydrated form of 3-oxoalanine as a catalytic nucleophile. Known substrates include GALNS, ARSA, STS and ARSE.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteinyl-[sulfatase] + 2 a thiol + O2 = an organic disulfide + 3-oxo-L-alanyl-[sulfatase] + hydrogen sulfide + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51152"/>
            <dbReference type="Rhea" id="RHEA-COMP:12900"/>
            <dbReference type="Rhea" id="RHEA-COMP:12901"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29256"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:35489"/>
            <dbReference type="ChEBI" id="CHEBI:85621"/>
            <dbReference type="EC" id="1.8.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13">Protein modification; sulfatase oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer, homodimer and heterodimer with SUMF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase-modifying factor family.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.7" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A452GN33"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003117"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003591.1">
        <property type="protein sequence ID" value="ENSGAGP00000003117.1"/>
        <property type="gene ID" value="ENSGAGG00000002518.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120147">
        <property type="term" value="F:formylglycine-generating oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043687">
        <property type="term" value="P:post-translational protein modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1580.10:FF:000001">
        <property type="entry name" value="Sulfatase modifying factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1580.10">
        <property type="entry name" value="paralog of FGE (formylglycine-generating enzyme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051043">
        <property type="entry name" value="Sulfatase_Mod_Factor_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005532">
        <property type="entry name" value="SUMF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042095">
        <property type="entry name" value="SUMF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23150:SF19">
        <property type="entry name" value="FORMYLGLYCINE-GENERATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23150">
        <property type="entry name" value="SULFATASE MODIFYING FACTOR 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03781">
        <property type="entry name" value="FGE-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 20" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019097292" description="Formylglycine-generating enzyme" evidence="20">
        <location>
            <begin position="33"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase-modifying factor enzyme-like" evidence="21">
        <location>
            <begin position="90"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00072922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF03781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="374" mass="40652" checksum="E061D44900466841" modified="2019-05-08" version="1">MAAPRAGRVLLSCRRWVLVLGLALSSVPGTAASSSSSHTAVSKGTCGCSSGRGSGGEAAAAAARRYSSEANAPSPAGGPVTRKGPAAHSQMALIPTGMFTMGTDELEIQQDGEWPARKVHVSQFYMDRYEVSNVEFEEFVNATGYVTEAEKFGDSFVFEGMLSEQVKNEIQQVVAAAPWWMPVKGANWRQPEGPDSDISKRMDHPVLHVSWNDAVAYCTWAGKRLPTEAEWEYSCRGGLENKLFPWGNKLQPKGQHYANTWQGEFPISNTGEDGYKGTAPVTAFPPNGYGLYNIVGNAWEWTSDWWAVHHSAGETRNPKGPSSGTDRVKKGGSYMCHKSYCYRYRCAARSQNTPDSSASNLGFRCAADTLPALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNR1</accession>
    <name>A0A452GNR1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="3">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452GNR1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003372"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003886.1">
        <property type="protein sequence ID" value="ENSGAGP00000003372.1"/>
        <property type="gene ID" value="ENSGAGG00000002717.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004706">
        <property type="term" value="F:JUN kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12059">
        <property type="entry name" value="SH3_MLK1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000076">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000085">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035779">
        <property type="entry name" value="MLK1-3_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF39">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 13" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SH3" evidence="18">
        <location>
            <begin position="27"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="106"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="392"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="483" mass="54347" checksum="72D65639B3807041" modified="2019-05-08" version="1">MEAGHKALPSEEAGDGPEDGSQRWNNKANPFWTAVFDYEAAAEEELTLRRGDLVEVLSQDSTVSGDEGWWTGKIEDRVGVFPCDYVASGPPGGPYPLPLEISFQELLLDEIIGVGGFGKVYKGMWRGEEVAVKAARQDPDKDISTTAESVRQEARLFCMLHHPNIIALKAVCLQSPNLCLVMEYARGGALNRALAGRKVPPHVLVNWAVQIARGMNYLHNEAVVPIIHRDLKSINILILEKMEAGELGGRTLKITDFGLAREWHKTTKMSAAGTYAWMAPEVIRLSLFSKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEPFARLLAECWDPDPHARPPFGSILGRLVAIEHSAMFSMALDAFHSLQDDWKLEIQQMFCELRAKEKELRSREEELLRAAQEQKTQEEQLRRREQELAAREIDIVQRELHLLMDQLGQERPRVKGRKGHFKRSRLKLRDGNRISLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPT5</accession>
    <name>A0A452GPT5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">YTH domain-containing family protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the YTHDF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPT5"/>
    <dbReference type="SMR" id="A0A452GPT5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003702"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004283.1">
        <property type="protein sequence ID" value="ENSGAGP00000003702.1"/>
        <property type="gene ID" value="ENSGAGG00000003007.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.590.10:FF:000001">
        <property type="entry name" value="YTH domain family 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF65">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="YTH" evidence="7">
        <location>
            <begin position="389"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="241"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="559" mass="61168" checksum="D5F95FD8FC8B516A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQ90</accession>
    <name>A0A452GQ90_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQ90"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003988"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004645.1">
        <property type="protein sequence ID" value="ENSGAGP00000003988.1"/>
        <property type="gene ID" value="ENSGAGG00000003265.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22296">
        <property type="entry name" value="CBD_TRPV5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024862">
        <property type="entry name" value="TRPV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008346">
        <property type="entry name" value="TRPV5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582:SF25">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL SUBFAMILY V MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL ION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01767">
        <property type="entry name" value="ECACCHANNEL2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="9">
        <location>
            <begin position="5"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="163"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="163"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="227" mass="26560" checksum="BD1CB18AFBBE2B03" modified="2019-05-08" version="1">MPAFYVIFQTENPDELGHFYNYPIALFSTFELFLTIIDGPANYEVDLPFMYCITYSAFAVIATLLMLNLLIAMMGDTHWRVAHERDELWRAQVVATTVMLERKLPQCLWPRSGICGQEYGLGDRWYLRVEDRCEGRQHKMHRYMEAFKFQITEDFDKYSQNKQELEDNKDTEAPYRKNTPCSTPAESPSLSQRSSSHGWDILRHSTLGQLQGHVNLAMEKEEEVYHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRV0</accession>
    <name>A0A452GRV0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PLAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GRV0"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005206.1">
        <property type="protein sequence ID" value="ENSGAGP00000004439.1"/>
        <property type="gene ID" value="ENSGAGG00000003676.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01756">
        <property type="entry name" value="PLAT_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052970">
        <property type="entry name" value="Inner_ear_hair_cell_LOXHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901:SF7">
        <property type="entry name" value="OXYGEN-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901">
        <property type="entry name" value="PROTEIN CBG12474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="2">
        <location>
            <begin position="55"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="2">
        <location>
            <begin position="311"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="2">
        <location>
            <begin position="581"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="2">
        <location>
            <begin position="729"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="864" mass="97274" checksum="CDB521303E679294" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GS74</accession>
    <name>A0A452GS74_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004727"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005537.1">
        <property type="protein sequence ID" value="ENSGAGP00000004727.1"/>
        <property type="gene ID" value="ENSGAGG00000003886.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003990">
        <property type="term" value="F:acetylcholinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006581">
        <property type="term" value="P:acetylcholine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019695">
        <property type="term" value="P:choline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00312">
        <property type="entry name" value="Esterase_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000029">
        <property type="entry name" value="Acetylcholinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050654">
        <property type="entry name" value="AChE-related_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000997">
        <property type="entry name" value="Cholinesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918">
        <property type="entry name" value="ACETYLCHOLINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918:SF4">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00878">
        <property type="entry name" value="CHOLNESTRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018809442" description="Carboxylic ester hydrolase" evidence="6">
        <location>
            <begin position="30"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="7">
        <location>
            <begin position="35"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600997-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="572" mass="62820" checksum="08B8D3BA867863B2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSV3</accession>
    <name>A0A452GSV3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GSV3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004810"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005626.1">
        <property type="protein sequence ID" value="ENSGAGP00000004807.1"/>
        <property type="gene ID" value="ENSGAGG00000003950.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000005629.1">
        <property type="protein sequence ID" value="ENSGAGP00000004810.1"/>
        <property type="gene ID" value="ENSGAGG00000003950.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23829">
        <property type="entry name" value="RWD_RWDD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd24163">
        <property type="entry name" value="RWDD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017359">
        <property type="entry name" value="Phi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010541">
        <property type="entry name" value="Prp3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059181">
        <property type="entry name" value="RWDD2A-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15955">
        <property type="entry name" value="RWD DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15955:SF8">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06544">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038021">
        <property type="entry name" value="UCP038021_RWDD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="1">
        <location>
            <begin position="15"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="294" mass="33787" checksum="D388C5648AC8E036" modified="2019-05-08" version="1">MAKMTNLEEAEVQLSELDLLSSMFPDEDEFIVTDQLAVAELKHYIDNKTSEIPSSKVQFILNVKQEVADATMVMLSLSCALPLNYPAVLPEITVRSSSLSRSQQIQLNTDLKTYLKQNCSGEVCVLNAREWVKDHAAAYIDKELSSSSVMTLRTMQSEDVIFTRLWIYSHHIYNKHKRKNIVDWSKELALTGFSMPGKPGVICVEGPQSTCEEFWSRVRRLTWRRILIRHREDISLDGSHTEMQKQRKFSYFEEKVFDAHGARGNHMDLGQLYHFLDEKGCADVFQMYFGVEGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTH3</accession>
    <name>A0A452GTH3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Relaxin-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterodimer of a B chain and an A chain linked by two disulfide bonds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the insulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTH3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005341"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006214.1">
        <property type="protein sequence ID" value="ENSGAGP00000005341.1"/>
        <property type="gene ID" value="ENSGAGG00000004322.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04365">
        <property type="entry name" value="IlGF_relaxin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022352">
        <property type="entry name" value="Ins/IGF/rlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016179">
        <property type="entry name" value="Insulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051777">
        <property type="entry name" value="Insulin-like_neuro_ligands"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036438">
        <property type="entry name" value="Insulin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022353">
        <property type="entry name" value="Insulin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20968">
        <property type="entry name" value="ILGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20968:SF0">
        <property type="entry name" value="RELAXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00276">
        <property type="entry name" value="INSULINFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00078">
        <property type="entry name" value="IlGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56994">
        <property type="entry name" value="Insulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00262">
        <property type="entry name" value="INSULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019328255" description="Relaxin-3" evidence="10">
        <location>
            <begin position="24"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Insulin-like" evidence="11">
        <location>
            <begin position="30"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="153" mass="16701" checksum="24BC919D4EBD66AA" modified="2019-05-08" version="1">MQKLLLALAFGTLLSELRLGAESRNPPYGVKLCGREFIRAVIFTCGGSRWRRAGLLASIPGSGREVGCMMGDSLGREDSSETASSEWDASHLPGMLPHQDLSPDYHERWRGKPGRGLAEESWPLERAVRDIMAGLSTSCCKWGCSKSEISSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTR7</accession>
    <name>A0A452GTR7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTR7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005333"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006203.1">
        <property type="protein sequence ID" value="ENSGAGP00000005333.1"/>
        <property type="gene ID" value="ENSGAGG00000004311.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008392">
        <property type="term" value="F:arachidonate epoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019373">
        <property type="term" value="P:epoxygenase P450 pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF424">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019506208" evidence="13">
        <location>
            <begin position="23"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="392" mass="44160" checksum="C8C3EFD74DFD7781" modified="2019-05-08" version="1">MELVSASLLLLLCLCCILLASAWKSQSQSQQGQLPPGPTPLPLLGNFLQLDRTNVIRSLEKYGPVFTVHLGSRRVVVLCGYQAVKEALVDQAEEFSGRGEIPAFSKDFNGHGVVFANGERWRQLRRFSLTVLRNFGMGKRSIEERIQEEAQCLVEELRKTQGAPFDPIFNLSQAVSNIICSIVFGNRFDYKDEKFIALNKLIFKRFRSAGLTSAQLYNAFPGILEKIPGPHQTGSKCSQEIISFILERIKLQQATLDLNAPQNFIDCFLVKMEQEKQNPATEFSLENLVMATFNLFFAGTETISTTLRYGFLILLKYPQVQGKEIDVVIGRDRAPTAEDRGRMPYTDATLHEIQRFSDILPMSLPHTVIRDTWFRGYLIPQVRPPGRGAGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUK1</accession>
    <name>A0A452GUK1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AXH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATXN1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GUK1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005762"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006720.1">
        <property type="protein sequence ID" value="ENSGAGP00000005762.1"/>
        <property type="gene ID" value="ENSGAGG00000004688.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.16.10">
        <property type="entry name" value="Hedgehog/Intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020997">
        <property type="entry name" value="Ataxin-1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043404">
        <property type="entry name" value="ATAXIN1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003652">
        <property type="entry name" value="Ataxin_AXH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036096">
        <property type="entry name" value="Ataxin_AXH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13392">
        <property type="entry name" value="ATAXIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13392:SF6">
        <property type="entry name" value="ATAXIN-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12547">
        <property type="entry name" value="ATXN-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08517">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00536">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102031">
        <property type="entry name" value="AXH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51148">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AXH" evidence="10">
        <location>
            <begin position="459"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="242"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="434"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="604"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="257"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="459"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="618"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="636"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="695" mass="74969" checksum="DD515C4735D187CA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUX8</accession>
    <name>A0A452GUX8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GUX8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005565"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006475.1">
        <property type="protein sequence ID" value="ENSGAGP00000005565.1"/>
        <property type="gene ID" value="ENSGAGG00000004508.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF163">
        <property type="entry name" value="ZINC FINGER PROTEIN 589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="11"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="137" mass="15568" checksum="B38CAA936A378664" modified="2019-05-08" version="1">IVGLLLFQGLVTFEEVAVYFTREEWALLDPTQRALYRDVMQENYETVVLLVSDKFHSPPLFSFSEKKVLPGAACTGEELIKPAQKLFGNAGNIWDALQRPCELSKFRIVPCRCKIIMADVTHGFPPILTDNWQKVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXE8</accession>
    <name>A0A452GXE8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXE8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006818"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007913.1">
        <property type="protein sequence ID" value="ENSGAGP00000006818.1"/>
        <property type="gene ID" value="ENSGAGG00000005497.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF160">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="455"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="541"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="10">
        <location>
            <begin position="371"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="588" mass="63884" checksum="C6C83ED5BEF53604" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXN9</accession>
    <name>A0A452GXN9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GXN9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006883"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007983.1">
        <property type="protein sequence ID" value="ENSGAGP00000006883.1"/>
        <property type="gene ID" value="ENSGAGG00000005549.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF57">
        <property type="entry name" value="OUTER DENSE FIBER OF SPERM TAILS 3-LIKE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="230" mass="24964" checksum="89A7963A864ECAD1" modified="2019-05-08" version="1">MAEVGRRKTPAITAHETGPRPGQYRLPPTVGFVNHDYTKLTSPDYSFHRRLNNGMYFKDSSPGPCYYVDLQLTRFGRSGGPSYSMLARSKTLAQPRTPGPGRYSPEKAPPVTQRRPPSFTMGSRTKYRWVVPVPAANSYTLPSLLGSRVSRSHRAMLWNSSPPSQSGLWGASSPLEQTCSGQEAHTASPPGSLLGAFSILCPSVRFPQRVHPSGVLGKPPGSAPPLCSQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYS4</accession>
    <name>A0A452GYS4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GYS4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007278"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008417.1">
        <property type="protein sequence ID" value="ENSGAGP00000007278.1"/>
        <property type="gene ID" value="ENSGAGG00000005772.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05763">
        <property type="entry name" value="IgI_LRIG1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000161">
        <property type="entry name" value="Leucine rich repeats and immunoglobulin like domains 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000040">
        <property type="entry name" value="Leucine rich repeats and immunoglobulin like domains 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000150">
        <property type="entry name" value="Leucine rich repeats and immunoglobulin like domains 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000224">
        <property type="entry name" value="Leucine rich repeats and immunoglobulin like domains 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000023">
        <property type="entry name" value="Leucine rich repeats and immunoglobulin like domains 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF16">
        <property type="entry name" value="LEUCINE-RICH REPEATS AND IMMUNOGLOBULIN-LIKE DOMAINS 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01463">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018990263" description="Ig-like domain-containing protein" evidence="13">
        <location>
            <begin position="28"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="777"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="471"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="575"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="669"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1097" mass="121314" checksum="7316CFA3346203A8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYT8</accession>
    <name>A0A452GYT8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein spinster homolog 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Spns1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine(out) + H(+)(out) = a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:74447"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:77287"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine(out) + H(+)(out) = a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:74455"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:77288"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1-acyl-sn-glycero-3-phosphocholine(out) + H(+)(out) = a 1-acyl-sn-glycero-3-phosphocholine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:74435"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1-acyl-sn-glycero-3-phosphoethanolamine(out) + H(+)(out) = a 1-acyl-sn-glycero-3-phosphoethanolamine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:74439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64381"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GYT8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007276"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008415.1">
        <property type="protein sequence ID" value="ENSGAGP00000007276.1"/>
        <property type="gene ID" value="ENSGAGG00000005846.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF13">
        <property type="entry name" value="PROTEIN SPINSTER HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="17">
        <location>
            <begin position="55"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="204" mass="22170" checksum="B2A027E0E753C9BC" modified="2019-05-08" version="1">MATRHGPHPDAAPFLSQADETEADAAEGGSRADGGQPEVGVHSVTGISYSRSVMIVAVLCYINLLNYMDRFTVAGILPDIESFFRIGDSSSGLLQTVFMSSYMVLAPIFGYLGDRYHRKRLLCLGIAFWSAVTLGSSFIPQEVPAGRPRAGRVQCGDEGLERCRGCSWANSVPLLNAEFLLVGILHQKNSEFCKKNFICLCTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZ02</accession>
    <name>A0A452GZ02_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GZ02"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008546.1">
        <property type="protein sequence ID" value="ENSGAGP00000007397.1"/>
        <property type="gene ID" value="ENSGAGG00000005939.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="72" mass="8128" checksum="ED6A49857B7C91AE" modified="2019-05-08" version="1">MSLSVCIPICLHVSTFIHKSMCACLCLHVYMHMCMCLCICMHRKLFFIFSTKPALAVPTAYLLKTLKCITGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZI6</accession>
    <name>A0A452GZI6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">U4/U6.U5 tri-snRNP-associated protein 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of a tri-snRNP complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SNUT3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZI6"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008870.1">
        <property type="protein sequence ID" value="ENSGAGP00000007686.1"/>
        <property type="gene ID" value="ENSGAGG00000006150.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013957">
        <property type="entry name" value="SNRNP27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31077">
        <property type="entry name" value="U4/U6.U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 27 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31077:SF1">
        <property type="entry name" value="U4_U6.U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 27 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08648">
        <property type="entry name" value="SNRNP27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U4/U6.U5 small nuclear ribonucleoprotein 27kDa protein" evidence="11">
        <location>
            <begin position="99"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="156" mass="18946" checksum="061ED9034900ED0E" modified="2019-05-08" version="1">MVRSRSRSPPRRERRRSRSASRERERRRRERSRSRERDRRRSRSRSPHRRRSRSPRRHRSSSSSPSRQKERRDDEKKENKDTKSKEHQITEEDMEGKTEEEIEMMKTMGFASFDTTKGKKVDGSVNAYAINVSQKRKYRQYMNRKGGFNRPLDFIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0G7</accession>
    <name>A0A452H0G7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Spartin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Lipophagy receptor that plays an important role in lipid droplet (LD) turnover in motor neurons. Localizes to LDs and interacts with components of the autophagy machinery, such as MAP1LC3A/C proteins to deliver LDs to autophagosomes for degradation via lipophagy. Lipid transfer protein required for lipid droplet degradation, including by lipophagy. Can bind and transfer all lipid species found in lipid droplets, from phospholipids to triglycerides and sterol esters but the direction of lipid transfer by spartin and its cargos are unknown. May be implicated in endosomal trafficking, or microtubule dynamics, or both. Participates in cytokinesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with ITCH and WWP1. Interacts (via MIT domain) with IST1; leading to the recruitment of SPART to midbodies. Interacts with MAP1LC3A and MAP1LC3C.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H0G7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007936"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009147.1">
        <property type="protein sequence ID" value="ENSGAGP00000007936.1"/>
        <property type="gene ID" value="ENSGAGG00000006322.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061724">
        <property type="term" value="P:lipophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02679">
        <property type="entry name" value="MIT_spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000009">
        <property type="entry name" value="spartin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009686">
        <property type="entry name" value="Senescence/spartin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045036">
        <property type="entry name" value="Spartin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068:SF43">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06911">
        <property type="entry name" value="Senescence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="MIT" evidence="19">
        <location>
            <begin position="18"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="142"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00067916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="631" mass="68752" checksum="2BEA39E86BC87729" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1E3</accession>
    <name>A0A452H1E3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H1E3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008432"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009708.1">
        <property type="protein sequence ID" value="ENSGAGP00000008432.1"/>
        <property type="gene ID" value="ENSGAGG00000006705.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="20"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="226" mass="25743" checksum="622BCC58C10AC456" modified="2019-05-08" version="1">CLEYQVAVLDALDLSPETFRQRFRGQTYPTVPCPRLIAQGLKEACRRWLQPETHTAEEVTEQMVLEQFVYILPARGRAWVLRHWPATLGAAVSLMEDFLAAEAPVWPAFRPPALVPDQSRGGRRVSAAAAKPRTGPRLAAIDGSVIDPGRATQWPHFELCRDRLYRVERDPRTQELRMQLLVLQCHRRAVMSLAHDIPAAGHLEYENTLSRIRARFYWPGLDGEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1N5</accession>
    <name>A0A452H1N5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H1N5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008301"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009558.1">
        <property type="protein sequence ID" value="ENSGAGP00000008301.1"/>
        <property type="gene ID" value="ENSGAGG00000006595.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070513">
        <property type="term" value="F:death domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097190">
        <property type="term" value="P:apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024130">
        <property type="entry name" value="DAP1/DAPL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13177">
        <property type="entry name" value="DEATH-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13177:SF2">
        <property type="entry name" value="DEATH-ASSOCIATED PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15228">
        <property type="entry name" value="DAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="108" mass="12072" checksum="7443390F7F6600E4" modified="2019-05-08" version="1">MSNHIQELLSYINCCLLTVKSGGRRVSKKQENGAVEKNSKNLGKEKTSSIVSVTKMQNMGVLLADALEKISHKFPAAAAQMAHQKPRPALQKSIWPKRIYIIQQPRKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1P0</accession>
    <name>A0A452H1P0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H1P0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008306"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009563.1">
        <property type="protein sequence ID" value="ENSGAGP00000008306.1"/>
        <property type="gene ID" value="ENSGAGG00000006588.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050900">
        <property type="term" value="P:leukocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002369">
        <property type="entry name" value="Integrin_bsu_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF36">
        <property type="entry name" value="INTEGRIN BETA-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00362">
        <property type="entry name" value="Integrin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00187">
        <property type="entry name" value="INB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0401">Integrin</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Integrin beta subunit VWA" evidence="16">
        <location>
            <begin position="4"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="451" mass="49308" checksum="2F0A274355C41D07" modified="2019-05-08" version="1">MPGSCGECPPAAESPSPLQNFTKVGESDATRCATREELLQRGCALEEVVEPWGRHQVLEDTPLSDSAEQEMVTQLAPQKIALWLRPGEQHSFSVRFKRAEGYPVDVYYLMDLSYSMKDDLENIKKLGNDLLAALRNITKSVKIGFGSFVDKTVLPYVSTVPAKLRNPCPDRYEQCDSPVSFRHVLPLTDNASEFESRVSQQRISGNLDSPEGGFDAIMQAAVCEEQIGWRNVTRLLVFTSDDVFHTAGDGKLGGIYLPNDNQCHLDTDGLYSKSHIYDYPSVGHLAQVLSASNIQPIFAVTGSTLPVYQELSRLIPKSVVGELKEDSSNVVQLISDAYNSLSSTVNLEHFQLPPGVSVAYESHCEDATRSLGGHSGVCSGVRINQLVSFTVTVQADTCLEGPHSFALRVLGFTEEVHVELQTLCECPCDKPEPHATHCSGGHGTLTCGVCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2F6</accession>
    <name>A0A452H2F6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H2F6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008762"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010069.1">
        <property type="protein sequence ID" value="ENSGAGP00000008762.1"/>
        <property type="gene ID" value="ENSGAGG00000006926.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040391">
        <property type="entry name" value="BEND5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14628">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14628:SF1">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="142" mass="16389" checksum="A44997CE39D5C1E3" modified="2019-05-08" version="1">MYAFVRFLEDNVCYALPVSCVRDFSPTSRLDFDNQKVYTVYRNPREAEEEDEESPGDWGERLLLHKAQILALAEDKTDLENSVMQKKIKIPKLSLNHVEEAGEVTDYGEEDVELRHSKVLKFFCFFGVFVCVKVLGAYAHMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2Y8</accession>
    <name>A0A452H2Y8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H2Y8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008976"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010310.1">
        <property type="protein sequence ID" value="ENSGAGP00000008976.1"/>
        <property type="gene ID" value="ENSGAGG00000007093.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="4"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="54" mass="6163" checksum="8A58E17AB609D9AD" modified="2019-05-08" version="1">MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3S8</accession>
    <name>A0A452H3S8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Luc7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3S8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009100"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010441.1">
        <property type="protein sequence ID" value="ENSGAGP00000009100.1"/>
        <property type="gene ID" value="ENSGAGG00000007181.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004882">
        <property type="entry name" value="Luc7-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12375">
        <property type="entry name" value="RNA-BINDING PROTEIN LUC7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03194">
        <property type="entry name" value="LUC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="235"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="130"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="235"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="289"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="313"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="369"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="470" mass="55786" checksum="1F3961BF77E63CA0" modified="2019-05-08" version="1">MISAAQLLDELMGRDRNLAPDEKRSNVRWDHESVCKYYLCGFCPAELFTNTRSDLGPCEKIHDENLRKQYEKSSRFMKVGYERDFLRYLQSLLAEVERRIRRGHARLALSQNQQSSGQAAGPTGKNEEKIQVLTDKIDVLLQQIEELGSEGKVEEAQGMMKLVEQLKEERELLRSTTSTIESFAAQEKQMEVCEVCGAFLIVGDAQSRVDDHLMGKQHMGYAKIKATVEELKEKLRKRTEEPERDDKLKKEKQEREEREKEREREREERERKRRREEEEKEKERARDRERRKRSRSRSRHSSRTSDRRCSRSRDHKRSRSRERRRSRSRDRRRSRSHDRSERKHRSRSRDRRRSKSRDRKSYKHRSKSRDRDQDRKSKEKEKRASDDKKSSMKSSSREKQSEDRNTDSKDSDTKNEVNGTNEDIKSEVQRKYAQMKMELSQVRRHTKAPSEGKDSVVLQNILSVGVVSGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4A5</accession>
    <name>A0A452H4A5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">6-pyruvoyltetrahydropterin synthase</fullName>
            <ecNumber evidence="4">4.2.3.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTPS family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.12" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452H4A5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009433"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010837.1">
        <property type="protein sequence ID" value="ENSGAGP00000009433.1"/>
        <property type="gene ID" value="ENSGAGG00000007460.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00849">
        <property type="reaction ID" value="UER00819"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003874">
        <property type="term" value="F:6-pyruvoyltetrahydropterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="entry name" value="6-pyruvoyl tetrahydropterin synthase/QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589:SF7">
        <property type="entry name" value="6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0783">Tetrahydrobiopterin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="82" mass="9178" checksum="B3130B123C1C9D43" modified="2019-05-08" version="1">MVINLTDCKEDTIMKLLDHKKLDQDVAYFADVVSTTENVAVYTWENLQKHLPAGQNVIVYNGDETAPLALMCDQSAHGVIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6Y9</accession>
    <name>A0A452H6Y9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H6Y9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010481"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012032.1">
        <property type="protein sequence ID" value="ENSGAGP00000010481.1"/>
        <property type="gene ID" value="ENSGAGG00000008200.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044224">
        <property type="term" value="C:juxtaparanode region of axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033270">
        <property type="term" value="C:paranode region of axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000007">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000021">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003972">
        <property type="entry name" value="K_chnl_volt-dep_Kv1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004048">
        <property type="entry name" value="K_chnl_volt-dep_Kv1.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF24">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01508">
        <property type="entry name" value="KV11CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01496">
        <property type="entry name" value="SHAKERCHANEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="19">
        <location>
            <begin position="35"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="493" mass="56345" checksum="995FBF43F5777439" modified="2019-05-08" version="1">MTVMAGENMDETSVLPGHPQDSYQPDAHDAHECCERVVINIAGLRFETQLKTLAQFPNTLLGNPKKRMRYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLRRPVNVPLDMFSEEIKFYELGEEAMEKFREDEGYIKEEEKPLPEKEYQRQVWLLFEYPESSGPARVIAIISVMVILISIVIFCLETLPDLKEDKDFTGTLQHIDNSTVIYKSNIFSDPFFIVETLCIIWFSFELVVRFFACPSKPEFFKNIMNFIDIVAIIPYFITLGTEMAEQEGTQKGEQATSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEAEEPESHFTSIPDAFWWAVVSMTTVGYGDMYPVTIGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETEGEEQAQLLHVSSPNLASHSDLSRRSSSTISKSEYMEIEEDMNNSIDNFREANLRTGNCTIANQNCVNKNKLLTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H989</accession>
    <name>A0A452H989_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H989"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011373"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013019.1">
        <property type="protein sequence ID" value="ENSGAGP00000011373.1"/>
        <property type="gene ID" value="ENSGAGG00000008785.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005031">
        <property type="term" value="F:tumor necrosis factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15838">
        <property type="entry name" value="TNFRSF27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000003">
        <property type="entry name" value="Tumor necrosis factor receptor superfamily member 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022319">
        <property type="entry name" value="TNFR_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034060">
        <property type="entry name" value="TNFRSF27_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047526">
        <property type="entry name" value="TNR19/27/EDAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12120">
        <property type="entry name" value="TNFR-CYS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12120:SF8">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00020">
        <property type="entry name" value="TNFR_c6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01973">
        <property type="entry name" value="TNFACTORR27"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57586">
        <property type="entry name" value="TNF receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019097294" description="TNFR-Cys domain-containing protein" evidence="12">
        <location>
            <begin position="20"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="9">
        <location>
            <begin position="26"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="13">
        <location>
            <begin position="26"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="13">
        <location>
            <begin position="67"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="9">
        <location>
            <begin position="67"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="298"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="540" mass="58538" checksum="34B4D2B51FCC925A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAQ1</accession>
    <name>A0A452HAQ1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in the correct development and proper functioning of the peripheral and central nervous system and be involved in cell adhesion and intercellular communication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the neurexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HAQ1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011804"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013518.1">
        <property type="protein sequence ID" value="ENSGAGP00000011804.1"/>
        <property type="gene ID" value="ENSGAGG00000009097.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1000:FF:000005">
        <property type="entry name" value="Contactin associated protein-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000016">
        <property type="entry name" value="Contactin-associated protein-like 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000026">
        <property type="entry name" value="contactin-associated protein-like 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF46">
        <property type="entry name" value="CONTACTIN-ASSOCIATED PROTEIN-LIKE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019534283" evidence="13">
        <location>
            <begin position="25"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="14">
        <location>
            <begin position="23"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="182"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="370"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="549"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="17">
        <location>
            <begin position="585"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="794"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="960"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="1003"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="932"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1264" mass="141116" checksum="8C2AABFB982EBE03" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAU9</accession>
    <name>A0A452HAU9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HAU9"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013591.1">
        <property type="protein sequence ID" value="ENSGAGP00000011872.1"/>
        <property type="gene ID" value="ENSGAGG00000009147.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="363" mass="41756" checksum="DE939B47443A9819" modified="2019-05-08" version="1">MDIIPNGLKVKNPLLSTYYTDHSERLCHTLSKKLRNHLISILYSKQENIKKELSNLETLINNQTSIQTDFTRIRQEIYITHFTSLQRKKDCKLSKLLPATWGHNSGTPNPPSNIVNLSNYTLSPEEKSVLSRGLSFCPATPTNMIQFCGDLEAYFRRLRLKEYFQDNTEQCTDTRVPSHQQHKKKNSTWTPPEGRNDSLDLYIECFRRRAQAEIVEQQHRLPHNLSRAERNAIHSLRNHPDIIIKEADKGGAVVIMNRSDYQKEAARQLSNTKFYRPLPSDPTEEYTKKLQHLLRTLPTLTPEEINIPLEPRPGLFYLLPKIHKPGNPGRPIISGIGTLTEGLSGYMDSLLRPYATSTPSYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBK2</accession>
    <name>A0A452HBK2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Retinoic acid receptor RXR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nuclear receptor subfamily 2 group B member</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for retinoic acid that acts as a transcription factor. Forms homo- or heterodimers with retinoic acid receptors (rars) and binds to target response elements in response to their ligands, all-trans or 9-cis retinoic acid, to regulate gene expression in various biological processes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Heterodimer; with a rar molecule.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HBK2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012141"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013906.1">
        <property type="protein sequence ID" value="ENSGAGP00000012141.1"/>
        <property type="gene ID" value="ENSGAGG00000009346.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003707">
        <property type="term" value="F:nuclear steroid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06956">
        <property type="entry name" value="NR_DBD_RXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06943">
        <property type="entry name" value="NR_LBD_RXR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000002">
        <property type="entry name" value="Retinoic acid receptor RXR-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000005">
        <property type="entry name" value="Retinoic acid receptor RXR-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021780">
        <property type="entry name" value="Nuc_recep-AF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000003">
        <property type="entry name" value="Retinoid-X_rcpt/HNF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11825">
        <property type="entry name" value="Nuc_recep-AF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00545">
        <property type="entry name" value="RETINOIDXR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="109"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="223"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="180"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="202"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="458" mass="50394" checksum="5A941681D6DF52A4" modified="2019-05-08" version="1">MAAPSLHPSIGPGIGSSLGSPGQLHSPISTLSSPINGMGPPFSVISSPMGPHSMSVPSTPSLGFGTGSPQLNSPMNPISSTEDIKPPLGLNGVLKVPAHPSGTMASFTKHICAICGDRSSGKHYGVYSCEGCKGFFKRTVRKDLTYTCRDNKDCLIDKRQRNRCQYCRYQKCLAMGMKREGMEHHPSRPTRLLGSQEEQAVQEERQRGKDRNENEVESTSSANEDMPVEKILEAELAVEPKTETYIEANMGLTPSSPNDPVTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRSIAVKDGILLATGLHVHRNSAHSAGVGAIFDRVLTELVSKMRDMQMDKTELGCLRAIVLFNPDSKGLSNPTEVEALREKVYASLEAYCKHKYPDQPGRFAKLLLRLPALRSIGLKCLEHLFFFKLIGDTPIDTFLMEMLEAPHQMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCN2</accession>
    <name>A0A452HCN2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the nuclear hormone receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HCN2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012548"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014382.1">
        <property type="protein sequence ID" value="ENSGAGP00000012548.1"/>
        <property type="gene ID" value="ENSGAGG00000009637.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07166">
        <property type="entry name" value="NR_DBD_REV_ERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000013">
        <property type="entry name" value="Nuclear receptor subfamily 1 group D member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000039">
        <property type="entry name" value="Nuclear receptor subfamily 1, group D, member 4b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF501">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 1, GROUP D, MEMBER 4A ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="12">
        <location>
            <begin position="108"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="13">
        <location>
            <begin position="331"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="547" mass="59953" checksum="2BA7AF8FED45AF3B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HD71</accession>
    <name>A0A452HD71_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HD71"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012744"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014606.1">
        <property type="protein sequence ID" value="ENSGAGP00000012744.1"/>
        <property type="gene ID" value="ENSGAGG00000009774.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12748">
        <property type="entry name" value="RRM4_RBM12B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047188">
        <property type="entry name" value="RRM4_RBM12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="173"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="424"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="614"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="269"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="507"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="555"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="690" mass="77682" checksum="9C86933613BC562C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDJ9</accession>
    <name>A0A452HDJ9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HDJ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012885"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014766.1">
        <property type="protein sequence ID" value="ENSGAGP00000012885.1"/>
        <property type="gene ID" value="ENSGAGG00000009888.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="74" mass="8073" checksum="A65872C6E7DF7960" modified="2019-05-08" version="1">MTRILTACKVVKTLKDGFEFCNVAAHHHWHLARTGTRFLSVKVIITKLSSPTNLPAPVLPDTEPSQATPGSPHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDU4</accession>
    <name>A0A452HDU4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Collagen alpha-1(VI) chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Collagen VI acts as a cell-binding protein.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Trimers composed of three different chains: alpha 1(VI), alpha 2(VI), and alpha 3(VI).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the type VI collagen family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HDU4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013016"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014913.1">
        <property type="protein sequence ID" value="ENSGAGP00000013016.1"/>
        <property type="gene ID" value="ENSGAGG00000009989.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005589">
        <property type="term" value="C:collagen type VI trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01480">
        <property type="entry name" value="vWA_collagen_alpha_1-VI-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000026">
        <property type="entry name" value="Collagen, type VI, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000050">
        <property type="entry name" value="Collagen, type VI, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000060">
        <property type="entry name" value="Collagen, type VI, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF87">
        <property type="entry name" value="COLLAGEN ALPHA-1(VI) CHAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019574725" description="Collagen alpha-1(VI) chain" evidence="14">
        <location>
            <begin position="24"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="41"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="355"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="564"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="760" mass="84132" checksum="61C615EC839B4464" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFR9</accession>
    <name>A0A452HFR9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HFR9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013675"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015653.1">
        <property type="protein sequence ID" value="ENSGAGP00000013675.1"/>
        <property type="gene ID" value="ENSGAGG00000010426.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="482"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="698" mass="78651" checksum="CA2AEC98ABFA3FBF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HG28</accession>
    <name>A0A452HG28_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JUPITER family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HG28"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013808"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015809.1">
        <property type="protein sequence ID" value="ENSGAGP00000013808.1"/>
        <property type="gene ID" value="ENSGAGG00000010522.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033335">
        <property type="entry name" value="JUPITER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930">
        <property type="entry name" value="GEO05313P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930:SF5">
        <property type="entry name" value="JUPITER MICROTUBULE ASSOCIATED HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="110"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="185" mass="19809" checksum="3D02BD39C5F98E4A" modified="2019-05-08" version="1">MFQGPEAGSAKPSSRVLKPPGGGSSNLFGNTEEVSSSSRPHRMASSIFGAAEEPQNFPKRTYPPGGKGSGIFEDPSPVQSRQRMNPPGGKTSDIFGSPVPASPVRAHPNKPKDHIVLQEAGDASKELKATENNKPQMEDRGEKRDLGKEEPGPKIDDHEPRLGPRPRSHNKVLNPPGGKSSIAFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HG63</accession>
    <name>A0A452HG63_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HG63"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013809"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015811.1">
        <property type="protein sequence ID" value="ENSGAGP00000013809.1"/>
        <property type="gene ID" value="ENSGAGG00000010524.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000015812.1">
        <property type="protein sequence ID" value="ENSGAGP00000013810.1"/>
        <property type="gene ID" value="ENSGAGG00000010524.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042752">
        <property type="term" value="P:regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22178">
        <property type="entry name" value="F-box_FBXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23957">
        <property type="entry name" value="FBXL3_LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000005">
        <property type="entry name" value="F-box/LRR-repeat protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000010">
        <property type="entry name" value="F-box/LRR-repeat protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10224">
        <property type="entry name" value="ES1 PROTEIN HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10224:SF18">
        <property type="entry name" value="F-BOX AND LEUCINE RICH REPEAT PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="11">
        <location>
            <begin position="38"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="433" mass="49223" checksum="6F1E711ACCE6796F" modified="2019-05-08" version="1">MKRGRKNSEANNSSSEEEVGGEFKKQKITDESTEISLSPDWGNLLPDIILQVFQYLSLLDRAHASQVCRRWNQVFHMPDLWRCFEFELNQPATSYLRATHPELINQIIKRHSNHLQYVSFKVDSSKESAEAACDILSQLVNCSLKTLGLISTARPSFMDLPKSHFISALTVVFVNSKSLSSLKIDDTPVDDPSLKVLVANNSDTLKLLKMSSCPHVSPAGILCVADHCHGLRELALNYHLLSDELLLALSSEKHVRLEHLRIDVVSENPGQTHFHTIQKSSWDAFIKHSPKVNLVMYFFLYEEEFDPFFRYETPVTHLYFGRSVSKDVLGRVGMTCPRLVELVVCANGLRPLDEELIRIAERCKNLSAIGLGECEVSCSAFVEFVKMCGGRLSQLSIMEEVLIPDQKYSLEQIHWEVSKHLGRVWFPDMMPTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HG75</accession>
    <name>A0A452HG75_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aspartate aminotransferase</fullName>
            <ecNumber evidence="10">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452HG75"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013881"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015890.1">
        <property type="protein sequence ID" value="ENSGAGP00000013881.1"/>
        <property type="gene ID" value="ENSGAGG00000010576.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006533">
        <property type="term" value="P:L-aspartate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000026">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000160">
        <property type="entry name" value="Similar to aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="21"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="393" mass="43937" checksum="6EDAFAA9A4AE6F6D" modified="2019-05-08" version="1">MGPPDPILGVTEAFKRDTNAKKMNLGVGAYRDDNGKPYVLNSVRKAEAQIAAKKMDKEYLPIAGLAEFNKASAELALGDTNEVIKSGRYVTVQTISGTGSLRVGANFLQRFFKYSRDVYLPKPSWGNHTPIFRDAGMQLHAYRYYDPKTCGFDFAGALDDISKIPEQSVILFHACAHNPTGVDPRPEQWKELAAVVKKRNLFAFFDMAYQGFASGDINRDSWAVRHFIEQGINVVLSQSYAKNMGLYGERVGAFSVICKDVDEAKRVESQLKILIRPMYSNPPINGARIASIILTSPDLRKEWLEEVKGMADRIIDMRNQLVANLKKEGSSHNWHHITDQIGMFCYTGLKPEQVERLTKEFSIYMTKDGRISVAGVTSGNVGYLARAIHQVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HG82</accession>
    <name>A0A452HG82_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HG82"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013833"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015837.1">
        <property type="protein sequence ID" value="ENSGAGP00000013833.1"/>
        <property type="gene ID" value="ENSGAGG00000010541.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15255">
        <property type="entry name" value="7tmB2_GPR144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000252">
        <property type="entry name" value="Adhesion G protein-coupled receptor D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.50:FF:000046">
        <property type="entry name" value="Adhesion G protein-coupled receptor D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000171">
        <property type="entry name" value="Adhesion G-protein coupled receptor D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032471">
        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF58">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16489">
        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="806"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="842"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="871"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="908"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="946"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="997"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1027"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="12">
        <location>
            <begin position="208"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="10">
        <location>
            <begin position="610"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="11">
        <location>
            <begin position="803"/>
            <end position="1050"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1149" mass="128842" checksum="868A13A0D083D4E1" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHY2</accession>
    <name>A0A452HHY2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Midkine</fullName>
            <shortName evidence="10">MK</shortName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Secreted protein that functions as cytokine and growth factor and mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Regulates many processes like inflammatory response, cell proliferation, cell adhesion, cell growth, cell survival, tissue regeneration, cell differentiation and cell migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the pleiotrophin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HHY2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014495"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016581.1">
        <property type="protein sequence ID" value="ENSGAGP00000014495.1"/>
        <property type="gene ID" value="ENSGAGG00000011017.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.60.10:FF:000002">
        <property type="entry name" value="Midkine a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.90.10:FF:000001">
        <property type="entry name" value="Pleiotrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.90.10">
        <property type="entry name" value="Heparin-binding Growth Factor, Midkine, Chain A- C-terminal Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.60.10">
        <property type="entry name" value="Pleiotrophin/Midkine, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000762">
        <property type="entry name" value="Midkine_heparin-bd_GF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020090">
        <property type="entry name" value="PTN/MK_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038130">
        <property type="entry name" value="PTN/MK_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020091">
        <property type="entry name" value="PTN/MK_diS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020089">
        <property type="entry name" value="PTN/MK_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037122">
        <property type="entry name" value="PTN/MK_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020092">
        <property type="entry name" value="PTN_MK_heparin-bd_GF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13850:SF2">
        <property type="entry name" value="MIDKINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13850">
        <property type="entry name" value="PLEIOTROPHIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01091">
        <property type="entry name" value="PTN_MK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05196">
        <property type="entry name" value="PTN_MK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00269">
        <property type="entry name" value="PTNMIDKINE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00193">
        <property type="entry name" value="PTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57288">
        <property type="entry name" value="Midkine"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00619">
        <property type="entry name" value="PTN_MK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00620">
        <property type="entry name" value="PTN_MK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 10" id="KW-0339">Growth factor</keyword>
    <keyword evidence="5 10" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="9 10" id="KW-0497">Mitogen</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019334428" description="Midkine" evidence="10">
        <location>
            <begin position="22"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Pleiotrophin/Midkine N-terminal" evidence="13">
        <location>
            <begin position="23"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Pleiotrophin/Midkine C-terminal" evidence="12">
        <location>
            <begin position="80"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="142" mass="15700" checksum="CFF2CEE09A32E453" modified="2019-05-08" version="1">MQVRGLFLLLVLILLAAASEAGKNKKDKVKKNGSDCEDWRWGPCTPNSKDCGVGYREGTCKEETKRLKCKIPCNWKKEFGADCKYKFESWGGCDAATGLKARSGTLKKALYNAECQETIQVTKPCSPKTKSKSKARKGKGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKX7</accession>
    <name>A0A452HKX7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP-4 complex subunit epsilon</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Adaptor protein complex 4 (AP-4) is a heterotetramer composed of two large adaptins, a medium adaptin and a small adaptin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HKX7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015583"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017799.1">
        <property type="protein sequence ID" value="ENSGAGP00000015583.1"/>
        <property type="gene ID" value="ENSGAGG00000011752.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030124">
        <property type="term" value="C:AP-4 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017109">
        <property type="entry name" value="AP4_complex_esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028269">
        <property type="entry name" value="AP4E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14807">
        <property type="entry name" value="AP4E_app_platf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037097">
        <property type="entry name" value="AP4_complex_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01356">
        <property type="entry name" value="AP4E_app_platf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP-4 complex subunit epsilon-1 C-terminal" evidence="7">
        <location>
            <begin position="965"/>
            <end position="1068"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1070" mass="120094" checksum="7FD703D1F013703A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLD2</accession>
    <name>A0A452HLD2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TALE/MEIS homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HLD2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015747"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017978.1">
        <property type="protein sequence ID" value="ENSGAGP00000015747.1"/>
        <property type="gene ID" value="ENSGAGG00000011836.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000004">
        <property type="entry name" value="Meis2 homeobox isoform 2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032453">
        <property type="entry name" value="PKNOX/Meis_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16493">
        <property type="entry name" value="Meis_PKNOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="182"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="184"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="319"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="325"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="368" mass="41118" checksum="3F8ACA87AFB8FA01" modified="2019-05-08" version="1">GNYSEFLHLQIVEIGEFHGNKNIYFLLPLMVKAIQVLRIHLLELEKVNELCKDFCNRYITCLKTKMHSDNLLRNDLGGPYSPNQPSINLHPQDLLQNSPNSMTAVANNPQGIVVPASALQPGNISMTTVNSQVVSGGALYQPVTMVTSQGQVVTQAIPQGAIQIQNTQVNLDLTSLLDNEDKKSKNKRGVLPKHATNIMRSWLFQHLMHPYPTEDEKRQIAAQTNLTLLQVNNWFINARRRILQPMLDASNPDPAPKAKKIKSQHRPTQRFWPNSIAAGVLQQQGVNPTTNPDGSLIMDNLQSLSSDNATIAMQQAMMAAHDDSLDGTEDEEEDEMEEEEEEELEEDNDELQTTNVSDLGLEHSDSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLS8</accession>
    <name>A0A452HLS8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease A-domain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pancreatic ribonuclease family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HLS8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018167.1">
        <property type="protein sequence ID" value="ENSGAGP00000015898.1"/>
        <property type="gene ID" value="ENSGAGG00000011965.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.130.10">
        <property type="entry name" value="Ribonuclease A-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001427">
        <property type="entry name" value="RNaseA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036816">
        <property type="entry name" value="RNaseA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023412">
        <property type="entry name" value="RNaseA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11437:SF10">
        <property type="entry name" value="ANGIOGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11437">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00074">
        <property type="entry name" value="RnaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00794">
        <property type="entry name" value="RIBONUCLEASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00092">
        <property type="entry name" value="RNAse_Pc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54076">
        <property type="entry name" value="RNase A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019164971" description="Ribonuclease A-domain domain-containing protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonuclease A-domain" evidence="6">
        <location>
            <begin position="27"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="150" mass="17282" checksum="7E06034024013EEE" modified="2019-05-08" version="1">MVPRGSCPLLFLTLVLLAICLAQNIVLPNSYERFVSEHVDFPKTRPPKGQSYCNVMMRCLCLIHPGCRPSNTFIHAPTRQVRAICSRPRICVYPSLNQCDSLTAFCLTTCQLVFNPSRPPRYIYREIPQTRRIHVACNQQQRPVHFLRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLU1</accession>
    <name>A0A452HLU1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Serine/threonine-protein kinase MARK2</fullName>
            <ecNumber evidence="8">2.7.11.1</ecNumber>
            <ecNumber evidence="7">2.7.11.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">ELKL motif kinase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">MAP/microtubule affinity-regulating kinase 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="38 39">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="39" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="38" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="38" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>L-seryl-[tau protein] + ATP = O-phospho-L-seryl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12801"/>
            <dbReference type="Rhea" id="RHEA-COMP:13701"/>
            <dbReference type="Rhea" id="RHEA-COMP:13702"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>L-threonyl-[tau protein] + ATP = O-phospho-L-threonyl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53904"/>
            <dbReference type="Rhea" id="RHEA-COMP:13703"/>
            <dbReference type="Rhea" id="RHEA-COMP:13704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lateral cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="8"/>
    <dbReference type="EC" id="2.7.11.26" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452HLU1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015904"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018173.1">
        <property type="protein sequence ID" value="ENSGAGP00000015904.1"/>
        <property type="gene ID" value="ENSGAGG00000011970.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016328">
        <property type="term" value="C:lateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14072">
        <property type="entry name" value="STKc_MARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14406">
        <property type="entry name" value="UBA_MARK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001032">
        <property type="entry name" value="KP78b, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000013">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000001">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049508">
        <property type="entry name" value="MARK1-4_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF56">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MARK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20 33" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="25" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="24" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="19" id="KW-0221">Differentiation</keyword>
    <keyword evidence="18" id="KW-0418">Kinase</keyword>
    <keyword evidence="22" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="21" id="KW-0460">Magnesium</keyword>
    <keyword evidence="23" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17 33" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="39" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="35">
        <location>
            <begin position="54"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="36">
        <location>
            <begin position="324"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="37">
        <location>
            <begin position="732"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="382"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="607"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="649"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="417"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="34">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="493"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="34">
        <location>
            <begin position="506"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="546"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="34">
        <location>
            <begin position="651"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00070360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00079159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00083985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="781" mass="87532" checksum="FC4962A57DB4D747" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMF3</accession>
    <name>A0A452HMF3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HMF3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016170"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018463.1">
        <property type="protein sequence ID" value="ENSGAGP00000016170.1"/>
        <property type="gene ID" value="ENSGAGG00000012125.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21270">
        <property type="entry name" value="CH_LRCH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000021">
        <property type="entry name" value="Leucine-rich repeat and calponin homology domain-containing protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000007">
        <property type="entry name" value="Leucine-rich repeat and calponin homology domain-containing protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF38">
        <property type="entry name" value="LEUCINE RICH REPEATS AND CALPONIN HOMOLOGY DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="455"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="244"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="607" mass="68912" checksum="5800A9B5D1A9F892" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQ26</accession>
    <name>A0A452HQ26_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQ26"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017139"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019562.1">
        <property type="protein sequence ID" value="ENSGAGP00000017139.1"/>
        <property type="gene ID" value="ENSGAGG00000012685.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF59">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORTER ABCD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="144"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="388"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="602" mass="68267" checksum="159A326C1BFEE62D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQU5</accession>
    <name>A0A452HQU5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S27</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQU5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017419"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019870.1">
        <property type="protein sequence ID" value="ENSGAGP00000017419.1"/>
        <property type="gene ID" value="ENSGAGG00000012954.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_eS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11594">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01168">
        <property type="entry name" value="RIBOSOMAL_S27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="85"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="116" mass="12740" checksum="281C7C5561111E68" modified="2025-10-08" version="2">MPLAKDLLHPSPEEEKWKYKKQHLAQSLNSYFMGVKCPGCYKITTVFSHAQTAVLCVGCSTVLCQPTGGKAKDLGCERRRVTTHQHAGGRAGHWAEESAVQPQQLQPKWDGSPGPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HR79</accession>
    <name>A0A452HR79_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PNKD metallo-beta-lactamase domain containing</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HR79"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017506"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019964.1">
        <property type="protein sequence ID" value="ENSGAGP00000017506.1"/>
        <property type="gene ID" value="ENSGAGG00000013006.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031833">
        <property type="entry name" value="DUF4748"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15932">
        <property type="entry name" value="DUF4748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="18559" checksum="46DC8AAB3EFB53A2" modified="2019-05-08" version="1">MRGPGKRALVCLPPRSPARRGARLAPPRAFGVTCLSWAGGFSAAVSRAVGLAGTRRPLARLNLRLIHGSCRAPLELRAKPEAGRRVHEIPEYIPTRKAKNPMKMVGVAWAIGFPSGIILFLLTKREVDKNRLKQLKARQKMKASNVGEYERARYRRSSLGPEGVADTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HU24</accession>
    <name>A0A452HU24_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaredoxin-related protein 5, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Monothiol glutaredoxin-5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HU24"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018613"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021221.1">
        <property type="protein sequence ID" value="ENSGAGP00000018613.1"/>
        <property type="gene ID" value="ENSGAGG00000013773.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000005">
        <property type="entry name" value="Glutaredoxin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00365">
        <property type="entry name" value="Grx4 family monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF16">
        <property type="entry name" value="GLUTAREDOXIN-RELATED PROTEIN 5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="9">
        <location>
            <begin position="86"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="189" mass="20553" checksum="970A088D9097964E" modified="2019-05-08" version="1">MGRRNGLEVDRSACGWRCKELQLSKAAMSGSLCWLRAASLLRGAPLRWGARPLSQAAAGDGSSPGGGEGGRGSRESVERLVRAHPVVVFMKGNPGQPLCGFSNAVVQILRLHGVQDFQAYDVLQDPDLRQGIKNYSNWPTIPQVYLNGEFVGGCDILLQMHQNGDLVEELKKLGIRSALLDAEKDQDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HU30</accession>
    <name>A0A452HU30_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HU30"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021152.1">
        <property type="protein sequence ID" value="ENSGAGP00000018552.1"/>
        <property type="gene ID" value="ENSGAGG00000013733.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="185" mass="19430" checksum="F5864CC970BA715D" modified="2019-05-08" version="1">TPRHPFMGRVPLPCPALSPPSFRPQGLPSPALCCACGLCIMLAGINITLVGAFAFGTFLPVNNPPIIIGPILLVVAFTFFGACCICSRRPPAHGARKSKPGSNTGLIKPGNTAFEIETSEHTVQDTTAVQLSPTNSPVSSRKSTPIHENSKTCKLFTMDGNGPAAKYTAGGESIQLNLPRDLMTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUD9</accession>
    <name>A0A452HUD9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[EGF-like domain protein] + UDP-alpha-D-glucose = 3-O-(beta-D-glucosyl)-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58116"/>
            <dbReference type="Rhea" id="RHEA-COMP:14610"/>
            <dbReference type="Rhea" id="RHEA-COMP:16010"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140576"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[EGF-like domain protein] + UDP-alpha-D-xylose = 3-O-(beta-D-xylosyl)-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62016"/>
            <dbReference type="Rhea" id="RHEA-COMP:16010"/>
            <dbReference type="Rhea" id="RHEA-COMP:16011"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132085"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KDELC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HUD9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018739"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021361.1">
        <property type="protein sequence ID" value="ENSGAGP00000018739.1"/>
        <property type="gene ID" value="ENSGAGG00000013864.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046527">
        <property type="term" value="F:glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000419">
        <property type="entry name" value="KDEL (Lys-Asp-Glu-Leu) containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF18">
        <property type="entry name" value="PROTEIN O-GLUCOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019457802" description="Glycosyl transferase CAP10 domain-containing protein" evidence="11">
        <location>
            <begin position="31"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="10">
        <location>
            <begin position="32"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="12">
        <location>
            <begin position="239"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="516" mass="59772" checksum="2F9CB0FFC9545524" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUQ9</accession>
    <name>A0A452HUQ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-C motif chemokine</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the intercrine beta (chemokine CC) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HUQ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018841"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021478.1">
        <property type="protein sequence ID" value="ENSGAGP00000018841.1"/>
        <property type="gene ID" value="ENSGAGG00000013939.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000827">
        <property type="entry name" value="Chemokine_CC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00472">
        <property type="entry name" value="SMALL_CYTOKINES_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="2 4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="5">
        <location>
            <begin position="24"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="158" mass="17250" checksum="9342C2E7DB127B79" modified="2019-05-08" version="1">QPSPCGASYQVRWTTPPTPVLNDALDCCLRTSHVPIPRKIVLDYAVQRIPDGCPIHAVVFITVRGKQLCAPPHAHWVRRLTERLDSVYQSKVRAHSQTRAKPTGTGRAAEAPVTQGWADTGEASPPPCWTQDAHCGSQLLTSPYGTSCMVTVFRIIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HV69</accession>
    <name>A0A452HV69_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HV69"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019039"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021701.1">
        <property type="protein sequence ID" value="ENSGAGP00000019039.1"/>
        <property type="gene ID" value="ENSGAGG00000014090.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22480">
        <property type="entry name" value="KH-I_FUBP3_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22483">
        <property type="entry name" value="KH-I_FUBP3_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22486">
        <property type="entry name" value="KH-I_FUBP3_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22489">
        <property type="entry name" value="KH-I_FUBP3_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000060">
        <property type="entry name" value="Far upstream element binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000007">
        <property type="entry name" value="far upstream element-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000008">
        <property type="entry name" value="far upstream element-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000010">
        <property type="entry name" value="far upstream element-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="90"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="175"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="266"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="367"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="439"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="465"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="559" mass="61191" checksum="54A5AAE5DC54A893" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYI4</accession>
    <name>A0A452HYI4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Serine protease HGFAC</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Serine protease that hydrolyzes the inactive zymogen hepatocyte growth factor (HGFsc) to an activated disulfide-linked heterodimer, then initiating hepatocyte growth factor receptor signaling pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer of a short chain and a long chain linked by a disulfide bond.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYI4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020202"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023017.1">
        <property type="protein sequence ID" value="ENSGAGP00000020202.1"/>
        <property type="gene ID" value="ENSGAGG00000014894.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00061">
        <property type="entry name" value="FN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00062">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00108">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000007">
        <property type="entry name" value="hepatocyte growth factor activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000338">
        <property type="entry name" value="hepatocyte growth factor activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000061">
        <property type="entry name" value="Hepatocyte growth factor activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000255">
        <property type="entry name" value="Sushi, nidogen and EGF-like domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000001">
        <property type="entry name" value="Urokinase-type plasminogen activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.10">
        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000083">
        <property type="entry name" value="Fibronectin_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000562">
        <property type="entry name" value="FN_type2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036943">
        <property type="entry name" value="FN_type2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF43">
        <property type="entry name" value="HEPATOCYTE GROWTH FACTOR ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00039">
        <property type="entry name" value="fn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00040">
        <property type="entry name" value="fn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00013">
        <property type="entry name" value="FNTYPEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00058">
        <property type="entry name" value="FN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00059">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01253">
        <property type="entry name" value="FN1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51091">
        <property type="entry name" value="FN1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00023">
        <property type="entry name" value="FN2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51092">
        <property type="entry name" value="FN2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 16" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 17" id="KW-0420">Kringle</keyword>
    <keyword evidence="5 19" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 19" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Fibronectin type-II" evidence="24">
        <location>
            <begin position="58"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="116"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="23">
        <location>
            <begin position="156"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="197"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="21">
        <location>
            <begin position="241"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="22">
        <location>
            <begin position="360"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="616" mass="69496" checksum="F1C7D9DAD3E481F4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYS8</accession>
    <name>A0A452HYS8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Palmitoyl-protein thioesterase 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HYS8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023236.1">
        <property type="protein sequence ID" value="ENSGAGP00000020394.1"/>
        <property type="gene ID" value="ENSGAGG00000015025.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019417669" description="Palmitoyl-protein thioesterase 2" evidence="1">
        <location>
            <begin position="31"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9199" checksum="E44FC95445D35997" modified="2019-05-08" version="1">MRGRPQGNWGALAPLCLLLFLFFLLTLGTSYKPVVVVHGLFDSPSEFSHLLLFINQVSPDAWTNPNSSSSSSIHGWVCVCVFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYZ2</accession>
    <name>A0A452HYZ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYZ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020459"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023308.1">
        <property type="protein sequence ID" value="ENSGAGP00000020459.1"/>
        <property type="gene ID" value="ENSGAGG00000015070.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000053">
        <property type="entry name" value="Importin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF26">
        <property type="entry name" value="IMPORTIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="9">
        <location>
            <begin position="22"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="883"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="883"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="896"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="928"/>
            <end position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1045" mass="120223" checksum="C2E4AF5D027796A0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0E3</accession>
    <name>A0A452I0E3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0389 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I0E3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020909"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023815.1">
        <property type="protein sequence ID" value="ENSGAGP00000020909.1"/>
        <property type="gene ID" value="ENSGAGG00000015377.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000023817.1">
        <property type="protein sequence ID" value="ENSGAGP00000020911.1"/>
        <property type="gene ID" value="ENSGAGG00000015377.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051402">
        <property type="term" value="P:neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090200">
        <property type="term" value="P:positive regulation of release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009432">
        <property type="entry name" value="DUF1075"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13674">
        <property type="entry name" value="GROWTH AND TRANSFORMATION-DEPENDENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13674:SF2">
        <property type="entry name" value="PROTEIN FAM162A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06388">
        <property type="entry name" value="DUF1075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="140" mass="15844" checksum="B00F15D8A2FEBDFF" modified="2019-05-08" version="1">MWAAAGRATKMLERNFSSILSPTKRMDLKIKRGFCSKSQEGSKQKPEVGGHSTFKVPGHKPTDWEKKVLMWSGRFKKVDDIPETVSLELIDAARNKLRVKMSYLMIAVTVLGCIVMVIEGKRAVGRHESLTTQNIERKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I102</accession>
    <name>A0A452I102_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox protein goosecoid-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Homeobox protein goosecoid-like</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May have a role in development. May regulate its own transcription. May bind the bicoid consensus sequence TAATCC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I102"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021183"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024124.1">
        <property type="protein sequence ID" value="ENSGAGP00000021183.1"/>
        <property type="gene ID" value="ENSGAGG00000015567.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000223">
        <property type="entry name" value="Goosecoid homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051440">
        <property type="entry name" value="Goosecoid-like_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46643:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN GOOSECOID-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46643">
        <property type="entry name" value="HOMEOBOX PROTEIN GOOSECOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="117"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="119"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="198" mass="22897" checksum="4F4EB72AFB380DA7" modified="2019-05-08" version="1">MSSEAPLDTELSRRSLKKQCSFTIENILSSPAERSPQVLAIAAGSLQEEEEEEEELEAAGCSCCCCSHASTRSLQDSSNWLDTRFPWPMRLIHPTARACKNLHGSQNELQTFHQIQRRTRRHRTIFTEDQLQALEALFNQNQYPDVITREHLANRIHLKEERVEVWFKNRRAKWRHQKRASASALLLQGTKKPSEENC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3J9</accession>
    <name>A0A452I3J9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3J9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022106"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025199.1">
        <property type="protein sequence ID" value="ENSGAGP00000022106.1"/>
        <property type="gene ID" value="ENSGAGG00000016237.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15225">
        <property type="entry name" value="7tmA_OR10A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="194"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="314" mass="34611" checksum="9A7A4297554DA41C" modified="2019-05-08" version="1">MPRGNESAVSQFLLLGFSSLEEWQVLLFLVFSPMYVIILTSNIAILAAISVDRSLHTPMYFLLFALSFSETCTTFAVVPKLLVNLLLGNQTISFLGCATQMFCFFGFGATNCFLLSAMAYDRYVAICKPLQYPVVITRRVCVELVAVSCATGFLIALGISSLVFSLPFCGPNAIKHFFCDISPILRLACSDHHIAGMAIVFLCAFVLLGPFTGIILSYIYILAAILKIHSAPGRRKTFSTCSSHLIVVITHFGCAFFVYLIPKSTSCLDEDTFIALSYTFFSPILNPVVYSLRNQEVKSALKKLTAHTFLSPKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3P6</accession>
    <name>A0A452I3P6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CREG-like beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CREG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3P6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022140"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025236.1">
        <property type="protein sequence ID" value="ENSGAGP00000022140.1"/>
        <property type="gene ID" value="ENSGAGG00000016260.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.110.10:FF:000004">
        <property type="entry name" value="Cellular repressor of E1A-stimulated genes 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014631">
        <property type="entry name" value="CREG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055343">
        <property type="entry name" value="CREG_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13343">
        <property type="entry name" value="CREG1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13343:SF21">
        <property type="entry name" value="PROTEIN CREG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13883">
        <property type="entry name" value="CREG_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036911">
        <property type="entry name" value="CREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018825393" description="CREG-like beta-barrel domain-containing protein" evidence="6">
        <location>
            <begin position="18"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="CREG-like beta-barrel" evidence="7">
        <location>
            <begin position="19"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR036911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="189" mass="20948" checksum="9E27E9B88846BCBA" modified="2019-05-08" version="1">MRLLLLLWALLAGEGAGIPPHEDAARVARYVVHQCDWGALATLSAREPLRGRPFANVFSLSDGPPGPRGSGVPYLYLTALEVSVQDLQVDANASLTVSLAQTPYCKKRGYDPQNPLCAHIIFSGTVDKVNDTEAVFAKLALFSRHPEMASWPRDHNWFFAKFNITNIWVLDYFGGLKTVTPQEYYNVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5L6</accession>
    <name>A0A452I5L6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LBH domain containing 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I5L6"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026077.1">
        <property type="protein sequence ID" value="ENSGAGP00000022888.1"/>
        <property type="gene ID" value="ENSGAGG00000016779.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037691">
        <property type="entry name" value="C11orf98"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14554:SF1">
        <property type="entry name" value="CHROMOSOME 11 OPEN READING FRAME 98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14554">
        <property type="entry name" value="GENE, 49416-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17719">
        <property type="entry name" value="DUF5564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="17922" checksum="0B5F24B2E1E75ED5" modified="2019-05-08" version="1">MNRKTSVTITSWSVTFQDPEVLSSGTARPGPRGCSMGTPGGKINRPRTELKKNLFKRRRVLSKEKRRKHRITGAVVDKGLITIHHLKKRASSSRANITLSGKKRRKLMKQIRHAAKEKATMQAEAVNLAQTKKITGSGRRKKPAASQDVEMKEAEAGPGLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5W4</accession>
    <name>A0A452I5W4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VLIG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Very large inducible GTPase (VLIG) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I5W4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022966"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026172.1">
        <property type="protein sequence ID" value="ENSGAGP00000022966.1"/>
        <property type="gene ID" value="ENSGAGG00000016849.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030383">
        <property type="entry name" value="G_VLIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057365">
        <property type="entry name" value="URGCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052986">
        <property type="entry name" value="VLIG_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819">
        <property type="entry name" value="GTP-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819:SF9">
        <property type="entry name" value="UP-REGULATOR OF CELL PROLIFERATION-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25496">
        <property type="entry name" value="URGCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25683">
        <property type="entry name" value="URGCP_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51717">
        <property type="entry name" value="G_VLIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VLIG-type G" evidence="2">
        <location>
            <begin position="590"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="669" mass="75503" checksum="F8A9DC09C536559B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7F1</accession>
    <name>A0A452I7F1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peripherin 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRPH2/ROM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I7F1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023559"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026846.1">
        <property type="protein sequence ID" value="ENSGAGP00000023559.1"/>
        <property type="gene ID" value="ENSGAGG00000017271.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03162">
        <property type="entry name" value="peripherin_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000002">
        <property type="entry name" value="Retinal outer segment membrane protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000830">
        <property type="entry name" value="Peripherin/rom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018498">
        <property type="entry name" value="Peripherin/rom-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042026">
        <property type="entry name" value="Peripherin_LEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF184">
        <property type="entry name" value="PERIPHERIN 2 LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00218">
        <property type="entry name" value="PERIPHERNRDS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00930">
        <property type="entry name" value="RDS_ROM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="41575" checksum="C15BE3B756043297" modified="2019-05-08" version="1">MAVFKVKFTKTKRHKLAQILWILNWISVATGVILFSLGLFLKIEIKKRSEVMAKGEINSVPNMLISVGVIACIINFLGGKICYDCSDTTKFSRWRLVMLPYIVCTFCFTFCILVGALTCYTMRNELEESLYLGLRDAIKFYKDTDIPGRCFLKKTIDLLQIGFQCCGNNGFRDWFEIQWISVRYLNMASKEVLDRLKSNVDGKFLVDGVPFSCCNPNSPRPCIQYHLTNNTAHYNYDFLMEELNIWMKGCRQALLDYYTDIMRSIGLTVLIIWLFEISVLTGVRYLQTAMKNVLLLGDPECESDGWLLENSFVETAKYNFSIIKNLGKANQISTVSEMNDPNMDVQTANYGPENVQSKSIPTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8G4</accession>
    <name>A0A452I8G4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I8G4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023949"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027273.1">
        <property type="protein sequence ID" value="ENSGAGP00000023949.1"/>
        <property type="gene ID" value="ENSGAGG00000017514.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001697">
        <property type="entry name" value="zinc finger protein 623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000038">
        <property type="entry name" value="Zinc finger protein 624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000431">
        <property type="entry name" value="zinc finger protein 629 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF421">
        <property type="entry name" value="ZINC FINGER PROTEIN 681 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="52"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="281" mass="31259" checksum="8589C15186F1FA91" modified="2019-05-08" version="1">ASSLPLHSKLASEGISCPGEHPTVLPTPEVLFLPSAGSKSWLDSLFIPAGDGMPRKNEEQKPLHEAPEVVEPPVTLSTRSQSPEWGGARETRCGSEKRQRNPLAQSWGKSARVGDGLTHQERPNMCLDCGKSFNKSSSLIIHQRTHTGERPYACPECGKRFTQSSTFLRHQRIHTGERPYDCAECGKSFRVSSHLVRHQRIHTGERPYVCTECGRSFSVSSHLVQHQRMHTGERPYRCACGKSFSLSSHLMHMLFSCQAAALTQTEPPRFQSSILSFTACH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8K2</accession>
    <name>A0A452I8K2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Shisa N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I8K2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023987"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027317.1">
        <property type="protein sequence ID" value="ENSGAGP00000023987.1"/>
        <property type="gene ID" value="ENSGAGG00000017542.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032591">
        <property type="term" value="C:dendritic spine membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026910">
        <property type="entry name" value="Shisa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053891">
        <property type="entry name" value="Shisa_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31774:SF14">
        <property type="entry name" value="PROTEIN SHISA-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31774">
        <property type="entry name" value="PROTEIN SHISA-9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13908">
        <property type="entry name" value="Shisa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018983040" description="Shisa N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="31"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Shisa N-terminal" evidence="8">
        <location>
            <begin position="70"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="414" mass="45583" checksum="8F783976D841B8CB" modified="2019-05-08" version="1">MASGSGAWMERGYIFGVCCLVLLDPGRVWTRAPSTQDPELERQVESRGNTSWAPTLEDTVVAPTEAVPGGDRCRGYYDVMGQWDPPFNCNSGIYRYCCGTCGYRFCCQFKHGRLDQSGCSNYDTPNWVNTGQPPARVDETPEDPTRDKTNMIVYIICGVVAIMVLVGIFTKLGLEKSWSPPTEMTMSRTLTDLLKQPGHGPSDHMTDGHVGSIQVQMGDGLARSSPRNGTESFFAFPDNMHLNNAVVSTPLAPQLGLPHAHSNRLQMTSTMPHQAPDYAKYATLKAVESAPEEFYKRFPMVDMPPPGPLSFPPMGLHQNDVPPLHDSCPLVGLATPGQKAKGVKTNAHPLVASPAFKGGWDPSHHHPPDVRRQIYANKRQFSVEKLPEVFSQTPTHYVQPSRPFSTNSKTEVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAZ5</accession>
    <name>A0A452IAZ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IAZ5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024902"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028346.1">
        <property type="protein sequence ID" value="ENSGAGP00000024902.1"/>
        <property type="gene ID" value="ENSGAGG00000018201.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07699">
        <property type="entry name" value="IgC1_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000283">
        <property type="entry name" value="Immunoglobulin kappa constant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF98">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="103"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="203" mass="23090" checksum="1D4F822B79622F3B" modified="2019-05-08" version="1">MWWACMDQDLALYELSQGILHCFTSQWIFIQSIVLRASPETMKLRSHGETSEVVPWPFSPHLQWWQPLPAIERDWFLYSRVSLWYVFGGGTQLTVLGQPKASPTVHLFPPSSEEIQTKSKATLVCLLGSFYPGSIQVTWKADGQQISTGVETTKPSKQSDNKYMASSYLSLDASDWKRHESYTCQVTHDGKNFEKSLKSSECS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAZ9</accession>
    <name>A0A452IAZ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Prominin 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prominin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IAZ9"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028345.1">
        <property type="protein sequence ID" value="ENSGAGP00000024901.1"/>
        <property type="gene ID" value="ENSGAGG00000018199.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031528">
        <property type="term" value="C:microvillus membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071914">
        <property type="term" value="C:prominosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008795">
        <property type="entry name" value="Prominin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730">
        <property type="entry name" value="PROMININ PROM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730:SF4">
        <property type="entry name" value="PROMININ-1-A-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05478">
        <property type="entry name" value="Prominin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="47092" checksum="91E8BB9C6BF98F6D" modified="2019-05-08" version="1">MALQNLSQPEYQPGPVPPAGSLPGLSSMVHSFLGLVQPNAFPAELLTNLIQQKNDITNPTVYREVLVYEVGFLVCVAIGLLFIILVPLVGFCFCCCRCCGNCGGTMYQKQSKRMGCRRRALFASVLLVTTLILAGNICAFISNDRVSRAVNSSFGALNTTLDNLGTFVRSIPQQVDFIIASSEVPVSQANGSLQSIGPILGGRIISEVGEEVYGALGSAMRLLEVTDLAEQELRAVNDSGQRLQQLQGELSQNLSRLQERINRTLQSCGQPCFQVSVSGLVPEADFSMVPDVSSQLALLSSLTSGNLSAALQQANETLKKTPTRVEEQAQKVVADARSQLAKVRQKIGGVRSEIPVLDTLGNVTAALDTIGRQARDYEPQVATYDGYRWIVGICLCCLVLLVVLCYLLALVLGGLGLKPLALPTERGCLSNSGGDFFMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICD7</accession>
    <name>A0A452ICD7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ICD7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025466"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028976.1">
        <property type="protein sequence ID" value="ENSGAGP00000025466.1"/>
        <property type="gene ID" value="ENSGAGG00000018608.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033299">
        <property type="term" value="P:secretion of lysosomal enzymes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000041">
        <property type="entry name" value="Tyrosine protein kinase receptor tie-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018711">
        <property type="entry name" value="NAGPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40446">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40446:SF2">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09992">
        <property type="entry name" value="NAGPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019487759" description="EGF-like domain-containing protein" evidence="8">
        <location>
            <begin position="41"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="528"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="425"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="588"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="607" mass="65711" checksum="A1C80D94B96D06BD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ID10</accession>
    <name>A0A452ID10_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 513</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Transcriptional regulator that plays a role in retinal development and maintenance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Binds DNA. Can associate with the proximal promoter regions of PAX6 and SP4, and their known targets including ARR3, RHO, OPN1MW2 and OPN1SW.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ID10"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025657"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029192.1">
        <property type="protein sequence ID" value="ENSGAGP00000025657.1"/>
        <property type="gene ID" value="ENSGAGG00000018725.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001967">
        <property type="entry name" value="Ras-responsive element-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000049">
        <property type="entry name" value="transcriptional repressor CTCF isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000123">
        <property type="entry name" value="transcriptional repressor CTCF isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001370">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000395">
        <property type="entry name" value="zinc finger protein 513"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000558">
        <property type="entry name" value="zinc finger protein 513 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050688">
        <property type="entry name" value="Zinc_finger/UBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056438">
        <property type="entry name" value="Znf-C2H2_CTCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403:SF66">
        <property type="entry name" value="ZINC FINGER PROTEIN 513"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23611">
        <property type="entry name" value="zf-C2H2_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="422"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="473"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="480"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="499" mass="55089" checksum="69266DAF48CCECCE" modified="2019-05-08" version="1">AIGEAVLPPLPVKCTRQMAPVDAEDTLEETPGGDLVLQSDLLLGQDLEFEDSSDRIMGFEKDLEGLSMGCVCFPEGPSYSLSDDDYSSYRQLSVESDPEEGREPGPTALPCSQCGLQLAVRVGPSCPQCAGAEEGRGQRVLYSCQLCPFASHYSSHLKRHMKTHNGEKPFKCPHCDYASAQLVNLTRHKRTHTGERPYRCQACSFACSSLGNLRRHERIHSQDKPFQCSACAYRCNQPPLTGPVSPSHNCPLPRPAGPLLPELNLQVSGASSPRLQGEEADALPELLFPFTCRMCGLVLDDGLVQDESLVEQICSRCSLAVLSDEPDESPPESGTKGFSCSLCPFATHYPNHLARHMKTHSGEKPFACPLCPYASAHLDNLKRHQRVHTGERPYKCQLCDYACGNLANLKRHGRIHSGDKPFRCSLCSYRCNQSMNLKRHMLRHTGEKPFRCHLCPYTTGHWDNYKRHQKIHGPAAEGWASPPSAKPRLALPPPAASLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHN2</accession>
    <name>A0A452IHN2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IHN2"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030686.1">
        <property type="protein sequence ID" value="ENSGAGP00000027004.1"/>
        <property type="gene ID" value="ENSGAGG00000019648.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="72" mass="8242" checksum="B2CC47E0DEA3C33B" modified="2019-05-08" version="1">MPATLLHLESMVPCRESQVSSLLSIFGEVARSVLCIWVDYRCSTKMFVLTDRLKTNLNILLLRARLTVYIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKM0</accession>
    <name>A0A452IKM0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">PLAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IKM0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028447"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032314.1">
        <property type="protein sequence ID" value="ENSGAGP00000028447.1"/>
        <property type="gene ID" value="ENSGAGG00000020618.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000006">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000011">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000030">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.60:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013275">
        <property type="entry name" value="Pept_M12B_ADAM-TS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF141">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01859">
        <property type="entry name" value="ADAMTS2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50900">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019497773" description="PLAC domain-containing protein" evidence="16">
        <location>
            <begin position="23"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="domain" description="PLAC" evidence="17">
        <location>
            <begin position="993"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="427"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="438"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="447"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="474"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="508"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="512"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="523"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1146" mass="130189" checksum="FEDD8FF85273B731" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKP3</accession>
    <name>A0A452IKP3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMEK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IKP3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028405"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032264.1">
        <property type="protein sequence ID" value="ENSGAGP00000028405.1"/>
        <property type="gene ID" value="ENSGAGG00000020307.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030289">
        <property type="term" value="C:protein phosphatase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000051">
        <property type="entry name" value="Serine/threonine-protein phosphatase 4 regulatory subunit 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055236">
        <property type="entry name" value="EVH1_PP4R3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006887">
        <property type="entry name" value="P4R3-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051137">
        <property type="entry name" value="PP4R3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318">
        <property type="entry name" value="ATP SYNTHASE GAMMA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318:SF18">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22972">
        <property type="entry name" value="EVH1_PP4R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04802">
        <property type="entry name" value="PP4R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PP4R3 EVH1-like" evidence="4">
        <location>
            <begin position="5"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine-protein phosphatase 4 regulatory subunit 3-like central" evidence="3">
        <location>
            <begin position="144"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="698"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="698"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="718"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="762"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="783"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="815"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="837" mass="95860" checksum="CF517608375FE975" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IL67</accession>
    <name>A0A452IL67_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Paired domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IL67"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028587"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032466.1">
        <property type="protein sequence ID" value="ENSGAGP00000028587.1"/>
        <property type="gene ID" value="ENSGAGG00000020697.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00131">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000013">
        <property type="entry name" value="Paired box 8 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000003">
        <property type="entry name" value="Paired box protein Pax-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043182">
        <property type="entry name" value="PAIRED_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001523">
        <property type="entry name" value="Paired_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022130">
        <property type="entry name" value="Pax2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF19">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00292">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12403">
        <property type="entry name" value="Pax2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00027">
        <property type="entry name" value="PAIREDBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00351">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00034">
        <property type="entry name" value="PAIRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51057">
        <property type="entry name" value="PAIRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0563">Paired box</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired" evidence="8">
        <location>
            <begin position="16"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="420" mass="45169" checksum="22E9B91539AFC9F2" modified="2019-05-08" version="1">MDMHCKADPFSAMHPGHGGVNQLGGVFVNGRPLPDVVRQRIVELAHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIKPGVIGGSKPKVATPKVVDKIAEYKRQNPTMFAWEIRDRLLAEGICDNDTVPSVSSINRIIRTKVQQPFHPTPDGTGTGVTAPGHTIVPSTASPPVSSASNDPVGSYSINGILGIPRSNGEKRKRDEVQIYTQTPLLRGWVGLTPCSLCLCPTDVSEGSVPNGDSQSSVDSLRKHLRADTFTQQQLEALDRVFERPSYPDVFQTSEHIKSEQGNEYSLPALTPGLDEVKSSLSASANPDLGTNVSGPQTYPVVTGRDMASTTLPGYPPHVPPTGQGSYPTSTLAGMVPGSEFSGNPYSHPQYTTYNEAWRFSNPALLSSPYYYSATSRGAAPPTAAAAYDRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILD6</accession>
    <name>A0A452ILD6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452ILD6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028655"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032547.1">
        <property type="protein sequence ID" value="ENSGAGP00000028655.1"/>
        <property type="gene ID" value="ENSGAGG00000020769.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10480">
        <property type="entry name" value="EphR_LBD_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000029">
        <property type="entry name" value="Ephrin type-A receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000023">
        <property type="entry name" value="Ephrin type-A receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000002">
        <property type="entry name" value="ephrin type-A receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000019">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000059">
        <property type="entry name" value="Ephrin type-A receptor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1770">
        <property type="entry name" value="ephrin a2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.510">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034263">
        <property type="entry name" value="EphA2_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001090">
        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050449">
        <property type="entry name" value="Ephrin_rcpt_TKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016257">
        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001426">
        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF20">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14575">
        <property type="entry name" value="EphA2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25599">
        <property type="entry name" value="Ephrin_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01404">
        <property type="entry name" value="Ephrin_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000666">
        <property type="entry name" value="TyrPK_ephrin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51550">
        <property type="entry name" value="EPH_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00790">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00791">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="12 22 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 22 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 26" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019282138" description="receptor protein-tyrosine kinase" evidence="26">
        <location>
            <begin position="27"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="537"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Eph LBD" evidence="30">
        <location>
            <begin position="28"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="29">
        <location>
            <begin position="325"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="29">
        <location>
            <begin position="433"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="615"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="28">
        <location>
            <begin position="904"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="21">
        <location>
            <position position="739"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <begin position="621"/>
            <end position="629"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22 24">
        <location>
            <position position="646"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="70"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="976" mass="109405" checksum="BAE7F046B02F9E68" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IR20</accession>
    <name>A0A452IR20_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COA8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IR20"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030546"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034664.1">
        <property type="protein sequence ID" value="ENSGAGP00000030546.1"/>
        <property type="gene ID" value="ENSGAGG00000021997.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097193">
        <property type="term" value="P:intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018796">
        <property type="entry name" value="COA8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31107">
        <property type="entry name" value="APOPTOGENIC PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31107:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10231">
        <property type="entry name" value="COA8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="199" mass="23001" checksum="F51844360F12A663" modified="2019-05-08" version="1">MAEAAAAAVRAGRRVRRVWSGTRRCFSRAGGGAGLLAAERGREPESRASSFCPPAHSCNDWIGPPDSQSNLRPIVFHIPKNESPLGRRLREFRQETQVWNQQFWASQNVSFRKGKEEFIYSRLKAKGLEMKGETGQKVTLSAEEMADFYKDFLSKNFKKHMCYNREWYKRNFTITFLMGQVAFERAWRRLGWKKTKVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRI6</accession>
    <name>A0A452IRI6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SEC14-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May play a role in innate immunity by inhibiting the antiviral RIG-I signaling pathway. In this pathway, functions as a negative regulator of RIGI, the cytoplasmic sensor of viral nucleic acids. Prevents the interaction of RIGI with MAVS/IPS1, an important step in signal propagation. May also regulate the SLC18A3 and SLC5A7 cholinergic transporters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with RIGI (via tandem CARD domain); the interaction is direct. Interacts (via GOLD domain) with SLC18A3; the interaction is direct. Interacts with SLC5A7 (via GOLD domain); the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IRI6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030611"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034733.1">
        <property type="protein sequence ID" value="ENSGAGP00000030611.1"/>
        <property type="gene ID" value="ENSGAGG00000022036.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039552">
        <property type="term" value="F:RIG-I binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039536">
        <property type="term" value="P:negative regulation of RIG-I signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.680:FF:000003">
        <property type="entry name" value="SEC14-like lipid binding 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000006">
        <property type="entry name" value="SEC14-like lipid binding 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.680">
        <property type="entry name" value="GOLD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051064">
        <property type="entry name" value="SEC14/CRAL-TRIO_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324">
        <property type="entry name" value="SEC14 RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324:SF51">
        <property type="entry name" value="SEC14-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="14">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CRAL-TRIO" evidence="12">
        <location>
            <begin position="285"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="13">
        <location>
            <begin position="487"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="685" mass="78251" checksum="F00D165700D841C8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITD7</accession>
    <name>A0A452ITD7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ITD7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031140"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035311.1">
        <property type="protein sequence ID" value="ENSGAGP00000031140.1"/>
        <property type="gene ID" value="ENSGAGG00000022372.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20869">
        <property type="entry name" value="C1_VAV3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21264">
        <property type="entry name" value="CH_VAV3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01223">
        <property type="entry name" value="PH_Vav"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10407">
        <property type="entry name" value="SH2_Vav3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11981">
        <property type="entry name" value="SH3_VAV3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000009">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000015">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000019">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000050">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000024">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037832">
        <property type="entry name" value="PH_Vav"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035881">
        <property type="entry name" value="VAV3_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047015">
        <property type="entry name" value="VAV3_SH3_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR VAV3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818">
        <property type="entry name" value="PROTEIN VAV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 9" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 10" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="15">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="14">
        <location>
            <begin position="192"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="400"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="16">
        <location>
            <begin position="513"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="591"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="11">
        <location>
            <begin position="671"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="815"/>
            <end position="875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="919" mass="106120" checksum="D33EAB23E05279BB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IU16</accession>
    <name>A0A452IU16_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IU16"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031479"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035703.1">
        <property type="protein sequence ID" value="ENSGAGP00000031479.1"/>
        <property type="gene ID" value="ENSGAGG00000022609.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="101" mass="11143" checksum="7ED765085B36EB2A" modified="2019-05-08" version="1">MSSDRQRSDDESPSTSSGSSDADQRDPPAPEPEEQEERKPSATQQKKNTKLSSKTTAKLSTSAKRIQKELAEITLDPPPNCSCDAIFILRKDFMLTVSIQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IU39</accession>
    <name>A0A452IU39_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IU39"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031619"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035860.1">
        <property type="protein sequence ID" value="ENSGAGP00000031619.1"/>
        <property type="gene ID" value="ENSGAGG00000022710.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000035865.1">
        <property type="protein sequence ID" value="ENSGAGP00000031622.1"/>
        <property type="gene ID" value="ENSGAGG00000022710.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000035870.1">
        <property type="protein sequence ID" value="ENSGAGP00000031627.1"/>
        <property type="gene ID" value="ENSGAGG00000022710.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000014">
        <property type="entry name" value="aminopeptidase O isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000008">
        <property type="entry name" value="aminopeptidase O isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.30:FF:000003">
        <property type="entry name" value="aminopeptidase O isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.320">
        <property type="entry name" value="Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033577">
        <property type="entry name" value="AOPep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038502">
        <property type="entry name" value="M1_LTA-4_hydro/amino_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015211">
        <property type="entry name" value="Peptidase_M1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46627">
        <property type="entry name" value="AMINOPEPTIDASE O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46627:SF1">
        <property type="entry name" value="AMINOPEPTIDASE O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09127">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01263">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal" evidence="8">
        <location>
            <begin position="690"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="799" mass="91265" checksum="9DD07FAD7621B60C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IU85</accession>
    <name>A0A452IU85_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IU85"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031603"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035841.1">
        <property type="protein sequence ID" value="ENSGAGP00000031603.1"/>
        <property type="gene ID" value="ENSGAGG00000022697.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF14">
        <property type="entry name" value="LEUCINE-RICH REPEAT, IMMUNOGLOBULIN-LIKE DOMAIN AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="227"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="339"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="514" mass="57838" checksum="381A4561FB7034FA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IV56</accession>
    <name>A0A452IV56_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphorylase b kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Glycan biosynthesis; glycogen metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Lipid-anchor</topology>
            <orientation evidence="1 7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the phosphorylase b kinase regulatory chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IV56"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031899"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036177.1">
        <property type="protein sequence ID" value="ENSGAGP00000031899.1"/>
        <property type="gene ID" value="ENSGAGG00000022485.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00163"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045583">
        <property type="entry name" value="KPBA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008734">
        <property type="entry name" value="PHK_A/B_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749:SF8">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19292">
        <property type="entry name" value="KPBB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6 7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 7" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="8">
        <location>
            <begin position="44"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphorylase b kinase regulatory subunit alpha/beta C-terminal" evidence="9">
        <location>
            <begin position="870"/>
            <end position="1028"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF19292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1044" mass="119979" checksum="846AF29397A81ED9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IVA7</accession>
    <name>A0A452IVA7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Polycomb group RING finger protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">DNA-binding protein Mel-18</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">RING finger protein 110</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Zinc finger protein 144</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Exists as both a monomer and homodimer. Component of a PRC1-like complex. Interacts with CBX8, RING1 and RNF2. Interacts with CBX7. Interacts with PHC2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IVA7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032051"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036339.1">
        <property type="protein sequence ID" value="ENSGAGP00000032051.1"/>
        <property type="gene ID" value="ENSGAGG00000022975.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000036340.1">
        <property type="protein sequence ID" value="ENSGAGP00000032052.1"/>
        <property type="gene ID" value="ENSGAGG00000022975.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17164">
        <property type="entry name" value="RAWUL_PCGF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16736">
        <property type="entry name" value="RING-HC_PCGF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000082">
        <property type="entry name" value="Polycomb group ring finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000170">
        <property type="entry name" value="Polycomb group RING finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032443">
        <property type="entry name" value="RAWUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10825:SF31">
        <property type="entry name" value="POLYCOMB GROUP RING FINGER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10825">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING, POLYCOMB GROUP COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16207">
        <property type="entry name" value="RAWUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="21">
        <location>
            <begin position="18"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="238"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="239"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="263"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="323"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="332" mass="37055" checksum="6D01809B18AD6506" modified="2019-05-08" version="1">MHRTTRIKITELNPHLMCALCGGYFIDATTIVECLHSFCKTCIVRYLETNKYCPMCDVQVHKTRPLLSIRSDKTLQDIVYKLVPGLFKDEMKRRRDFYAAYPMADVPNGSNEDRGEVSEQDKGNLTDDEIVSLSIEFYEGMRDEKKGMIENVDAEKEKKNGVRFLRCPAAMTVMHLAKFLRNKMDVPSKYKVEVLYEDEPLKEYYTLMDIAYIYPWRRNGPLPLKYRVQPACKRLKLSQPANSECTNTSGASECESVSDKANSPATLPATSSSLPSPATPSHGSPSSNTTTISIPANPGTILNGTSNCHQMPPSASRGRKMTVNGSTASALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IVJ8</accession>
    <name>A0A452IVJ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Manganese-dependent ADP-ribose/CDP-alcohol diphosphatase</fullName>
            <ecNumber evidence="5">3.6.1.13</ecNumber>
            <ecNumber evidence="4">3.6.1.16</ecNumber>
            <ecNumber evidence="6">3.6.1.53</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">ADPRibase-Mn</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">CDP-choline phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ADP-D-ribose + H2O = D-ribose 5-phosphate + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:10412"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ADP-D-ribose + H2O = D-ribose 5-phosphate + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:10412"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.53"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>CDP-choline + H2O = phosphocholine + CMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:32487"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="3.6.1.53"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>CDP-glycerol + H2O = sn-glycerol 3-phosphate + CMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:21692"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58311"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="3.6.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADPRibase-Mn family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.13" evidence="5"/>
    <dbReference type="EC" id="3.6.1.16" evidence="4"/>
    <dbReference type="EC" id="3.6.1.53" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452IVJ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031919"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036198.1">
        <property type="protein sequence ID" value="ENSGAGP00000031919.1"/>
        <property type="gene ID" value="ENSGAGG00000022909.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008663">
        <property type="term" value="F:2',3'-cyclic-nucleotide 2'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047734">
        <property type="term" value="F:CDP-glycerol diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07396">
        <property type="entry name" value="MPP_Nbla03831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041869">
        <property type="entry name" value="MPP_ADPRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16509">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16509:SF1">
        <property type="entry name" value="MANGANESE-DEPENDENT ADP-RIBOSE_CDP-ALCOHOL DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="17">
        <location>
            <begin position="21"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="346" mass="39287" checksum="2F8ED0BD4B5BD569" modified="2019-05-08" version="1">MDETPTWRPGSLTGNSELCFSFGVIADIQYADLEDGYNFCGSRRRYYRHSLNLLQSAVDEWNKEDSQLEFVLQLGDIIDGFNAQHKTSESALRRVMNKFKKLRIPIHHTWGNHELYNFTRDYLTQSELNSKSLEDQIFLGSAASGQDSPGATAEEHYYAYHFSPVPKFRFILLDAYDLSILGRDKSTQKYQDSLKLLKEKNPNENLNSPTGLAEPQFVEFNGGFSQAQLDWFNEVLTFSDTNQEKVVVVSHIPVHPGSTESVCLAWNYKDALSVIHSHQCVVCFLAGHLHDGGYYLDSHGVHHLTLEGIVETPPESHAFGTIHVYDDKLVLKGRGRIPDKVMYYTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWT9</accession>
    <name>A0A452IWT9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IWT9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032379"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036709.1">
        <property type="protein sequence ID" value="ENSGAGP00000032379.1"/>
        <property type="gene ID" value="ENSGAGG00000023170.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF11">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="5">
        <location>
            <begin position="334"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="6">
        <location>
            <begin position="749"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="280"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="475"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="939"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="50"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="280"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="535"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="580"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="986" mass="110727" checksum="C03E10866323C210" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IXP9</accession>
    <name>A0A452IXP9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IXP9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032848"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037231.1">
        <property type="protein sequence ID" value="ENSGAGP00000032848.1"/>
        <property type="gene ID" value="ENSGAGG00000023465.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="86" mass="9262" checksum="7138CA1CB962A8D9" modified="2019-05-08" version="1">MSSPASTPSRRGSKRGRSSNPQTPRTEDAQSPLLQKSRTDDSTSTGDLQPMPTSPAVDMQSPNAQDVLFSSPLQFWLRLSVVGAKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZ41</accession>
    <name>A0A452IZ41_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZ41"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033348"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037788.1">
        <property type="protein sequence ID" value="ENSGAGP00000033348.1"/>
        <property type="gene ID" value="ENSGAGG00000023774.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF7">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 11B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="246" mass="28022" checksum="4DEBEAB284060457" modified="2019-05-08" version="1">MDFWVQFSAQSQAKERVFRAAQYACTLLGYTLQKNGVSADFLTRIKQLEAHMSLGRKLFRLGNSADALESAKRAIHLSDVVLRFCITVSNLNRAMYFACDNILWAGKSGLLPHMDQEKWSQRSFRYYFFALILNLSRDAYEIRLLMEREVCGKRPKGSETRHALRADSSLQQLVLRLKVQLHLLVHVLRSNPPLLVDVVKNACDIFIPLDKLGLYKTNPGFVGLCGLTSSILSILTIIHPWLKLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1C9</accession>
    <name>A0A452J1C9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">S100P-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J1C9"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038712.1">
        <property type="protein sequence ID" value="ENSGAGP00000034186.1"/>
        <property type="gene ID" value="ENSGAGG00000024288.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026097">
        <property type="entry name" value="S100PBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14455">
        <property type="entry name" value="ASKOPOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14455:SF0">
        <property type="entry name" value="S100P-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15427">
        <property type="entry name" value="S100PBPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="260"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="385"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="385"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="571" mass="63455" checksum="2BAEBAF5D3BC2D49" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J409</accession>
    <name>A0A452J409_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Ephrin type-B receptor 3</fullName>
            <ecNumber evidence="3">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452J409"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035164"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039815.1">
        <property type="protein sequence ID" value="ENSGAGP00000035164.1"/>
        <property type="gene ID" value="ENSGAGG00000024991.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05065">
        <property type="entry name" value="PTKc_EphR_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09553">
        <property type="entry name" value="SAM_EPH-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00185">
        <property type="entry name" value="TNFRSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000041">
        <property type="entry name" value="ephrin type-A receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000015">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000004">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000520">
        <property type="entry name" value="ephrin type-B receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1770">
        <property type="entry name" value="ephrin a2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001090">
        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050449">
        <property type="entry name" value="Ephrin_rcpt_TKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016257">
        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001426">
        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF6">
        <property type="entry name" value="EPHRIN TYPE-B RECEPTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14575">
        <property type="entry name" value="EphA2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25599">
        <property type="entry name" value="Ephrin_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01404">
        <property type="entry name" value="Ephrin_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000666">
        <property type="entry name" value="TyrPK_ephrin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51550">
        <property type="entry name" value="EPH_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00790">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00791">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 25 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="17 26" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="15 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="10 25 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 28" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="16" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Eph LBD" evidence="32">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="31">
        <location>
            <begin position="269"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="31">
        <location>
            <begin position="382"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="29">
        <location>
            <begin position="562"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="30">
        <location>
            <begin position="854"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="24">
        <location>
            <position position="687"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <begin position="568"/>
            <end position="576"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25 27">
        <location>
            <position position="594"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="26">
        <location>
            <begin position="10"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="26">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00038546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00040789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="927" mass="102936" checksum="90FBA949FE056237" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5P1</accession>
    <name>A0A452J5P1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J5P1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035784"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040513.1">
        <property type="protein sequence ID" value="ENSGAGP00000035784.1"/>
        <property type="gene ID" value="ENSGAGG00000025397.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000040515.1">
        <property type="protein sequence ID" value="ENSGAGP00000035786.1"/>
        <property type="gene ID" value="ENSGAGG00000025397.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000040516.1">
        <property type="protein sequence ID" value="ENSGAGP00000035787.1"/>
        <property type="gene ID" value="ENSGAGG00000025397.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098560">
        <property type="term" value="C:cytoplasmic side of late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098574">
        <property type="term" value="C:cytoplasmic side of lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF46">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LITAF" evidence="9">
        <location>
            <begin position="67"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="152" mass="16348" checksum="D57FD64CFB119D3C" modified="2019-05-08" version="1">MSAPASHLPAPTGYPIPSAPPTYEETTGINTYPPYPAPESGHGPNMKSMNPPPYPSQPVPAPNPITVQTVYVQQPVTFYDRPVQMCCPSCNKMIVTRLSHTSGALTWLSCGSLCLLGCVAGCCFIPFCIDALQDVDHYCPSCQALLGTYKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5S6</accession>
    <name>A0A452J5S6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J5S6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035710"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040437.1">
        <property type="protein sequence ID" value="ENSGAGP00000035710.1"/>
        <property type="gene ID" value="ENSGAGG00000025357.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051832">
        <property type="entry name" value="mTOR-Rac_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22829">
        <property type="entry name" value="DEP DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22829:SF18">
        <property type="entry name" value="DEP DOMAIN-CONTAINING MTOR-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="2">
        <location>
            <begin position="26"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="256" mass="29074" checksum="78F5049C6FBF5022" modified="2019-05-08" version="1">MDQELLSEELLSPYIYFFSFHGRLMNSENTLLQAREEEGVKYERTVMASELIEWLIQEGEVTTRTEAEQLGRRLLEHGIIQHVSSKHHFMDSNLLYQFRMNFRRRRRLMELLPEKSRLMPESHDSPFCLRKQNHDNRKHTSFQSVSPTKEIKIVSAVRRSSMSSCGSSGYFSSSPTLSSSPPVLCNPKSVLKRPVTSDELLMPGAPYARKTFTVVGDAVGWGFVVRGSKPCHIQAVDPSGPAAAAGMKVIFHQLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J797</accession>
    <name>A0A452J797_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-hydroxyacyl-CoA lyase 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetolactate synthase-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">IlvB-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2R)-hydroxyhexadecanoyl-CoA = pentadecanal + formyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:55212"/>
            <dbReference type="ChEBI" id="CHEBI:17302"/>
            <dbReference type="ChEBI" id="CHEBI:57376"/>
            <dbReference type="ChEBI" id="CHEBI:138654"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:55213"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2-hydroxyoctadecanoyl-CoA = heptadecanal + formyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:55196"/>
            <dbReference type="ChEBI" id="CHEBI:57376"/>
            <dbReference type="ChEBI" id="CHEBI:74116"/>
            <dbReference type="ChEBI" id="CHEBI:138631"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:55197"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J797"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036387"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041208.1">
        <property type="protein sequence ID" value="ENSGAGP00000036387.1"/>
        <property type="gene ID" value="ENSGAGG00000025796.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02004">
        <property type="entry name" value="TPP_BZL_OCoD_HPCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000048">
        <property type="entry name" value="IlvB (bacterial acetolactate synthase)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF166">
        <property type="entry name" value="2-HYDROXYACYL-COA LYASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="15">
        <location>
            <begin position="52"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="13">
        <location>
            <begin position="272"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="14">
        <location>
            <begin position="466"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="411"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="631" mass="68069" checksum="131C92590364E883" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7M7</accession>
    <name>A0A452J7M7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J7M7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036520"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041358.1">
        <property type="protein sequence ID" value="ENSGAGP00000036520.1"/>
        <property type="gene ID" value="ENSGAGG00000025896.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019455705" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="21"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="121" mass="13342" checksum="FE3A4C909361F789" modified="2019-05-08" version="1">NTAWAASITLSLLPADVQTQPVLTQEPSVSIASGESVTLKCVMSLAKFKDYCVDWYQPGSPLRYLLYYNSEVDKDKPSVIPDRFSASKDLASNACVLTIAGVQAEDHADYYCSVSYPIYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GG70</accession>
    <name>A0A452GG70_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GG70"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000575"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000646.1">
        <property type="protein sequence ID" value="ENSGAGP00000000575.1"/>
        <property type="gene ID" value="ENSGAGG00000000463.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF142">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="4"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="94" mass="10353" checksum="28EFB76C51D95929" modified="2019-05-08" version="1">LTKVAFEEVAVYFTQGQGALLDPAQRALYRDVMQENYETVTSLGNPVPKPELITQLERGEELWVPDLQTSEEGEIPRGSHTGELCCLISSTSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GN98</accession>
    <name>A0A452GN98_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Period circadian-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GN98"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003326"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003831.1">
        <property type="protein sequence ID" value="ENSGAGP00000003326.1"/>
        <property type="gene ID" value="ENSGAGG00000002685.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001222">
        <property type="term" value="F:transcription corepressor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022728">
        <property type="entry name" value="Period_circadian-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050760">
        <property type="entry name" value="Period_circadian_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269:SF16">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12114">
        <property type="entry name" value="Period_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Period circadian-like C-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="164" mass="17909" checksum="C07678EBE121D8A2" modified="2025-10-08" version="2">MREMQKQQPRFTEEQKKELAEVHPWVKKGLLPKAINVMACVDCGSNPASRLAPLYDTEPHAMELSATLEPMEEGSGAPPCLDMAMEEEEVGAGCPSEPGTHTGWTRSAGPALAPGLSQVERRAEPGPAPDPTLPVGAPRRLELALRRVEYLFLKGFFLYEKAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPH8</accession>
    <name>A0A452GPH8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPH8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004425.1">
        <property type="protein sequence ID" value="ENSGAGP00000003822.1"/>
        <property type="gene ID" value="ENSGAGG00000002015.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038191">
        <property type="term" value="F:neuropilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05871">
        <property type="entry name" value="Ig_Sema3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11254">
        <property type="entry name" value="Sema_3F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000030">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000001">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF27">
        <property type="entry name" value="SEMAPHORIN-3F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sema" evidence="11">
        <location>
            <begin position="30"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="613"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="771"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="771"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="796" mass="89544" checksum="F9B95C2643589BE5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQ08</accession>
    <name>A0A452GQ08_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Platelet-derived growth factor receptor beta</fullName>
            <ecNumber evidence="4">2.7.10.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Beta platelet-derived growth factor receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Beta-type platelet-derived growth factor receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">CD140 antigen-like family member B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Platelet-derived growth factor receptor 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="44 45">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="45" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="44" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="44" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="30">Interacts with homodimeric PDGFB and PDGFD, and with heterodimers formed by PDGFA and PDGFB. May also interact with homodimeric PDGFC. Monomer in the absence of bound ligand. Interaction with homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD, leads to receptor dimerization, where both PDGFRA homodimers and heterodimers with PDGFRB are observed. Interacts with SH2B2/APS. Interacts directly (tyrosine phosphorylated) with SHB. Interacts (tyrosine phosphorylated) with PIK3R1 and RASA1. Interacts (tyrosine phosphorylated) with CBL. Interacts (tyrosine phosphorylated) with SRC and SRC family kinases. Interacts (tyrosine phosphorylated) with PIK3C2B, maybe indirectly. Interacts (tyrosine phosphorylated) with SHC1, GRB7, GRB10 and NCK1. Interaction with GRB2 is mediated by SHC1. Interacts (via C-terminus) with NHERF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="38">Membrane</location>
            <topology evidence="38">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="38">Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452GQ08"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003985"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004641.1">
        <property type="protein sequence ID" value="ENSGAGP00000003985.1"/>
        <property type="gene ID" value="ENSGAGG00000003261.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043202">
        <property type="term" value="C:lysosomal lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005019">
        <property type="term" value="F:platelet-derived growth factor beta-receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048407">
        <property type="term" value="F:platelet-derived growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902533">
        <property type="term" value="P:positive regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014911">
        <property type="term" value="P:positive regulation of smooth muscle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000025">
        <property type="entry name" value="Platelet-derived growth factor receptor alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000140">
        <property type="entry name" value="Platelet-derived growth factor receptor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000223">
        <property type="entry name" value="Platelet-derived growth factor receptor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000572">
        <property type="entry name" value="Platelet-derived growth factor receptor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000715">
        <property type="entry name" value="Platelet-derived growth factor receptor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000982">
        <property type="entry name" value="Platelet-derived growth factor receptor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001824">
        <property type="entry name" value="Tyr_kinase_rcpt_3_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF53">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR RECEPTOR BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25305">
        <property type="entry name" value="Ig_PDGFR_d4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000615">
        <property type="entry name" value="TyrPK_CSF1-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01832">
        <property type="entry name" value="VEGFRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00240">
        <property type="entry name" value="RECEPTOR_TYR_KIN_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 34 37" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="26" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="23" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="25 38" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="24" id="KW-0458">Lysosome</keyword>
    <keyword evidence="35" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19 40" id="KW-0472">Membrane</keyword>
    <keyword evidence="35" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 34 37" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22 38" id="KW-0675">Receptor</keyword>
    <keyword evidence="45" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 41" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 38 40" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18 40" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="20" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="41">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019474306" description="Platelet-derived growth factor receptor beta" evidence="41">
        <location>
            <begin position="33"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="40">
        <location>
            <begin position="535"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="43">
        <location>
            <begin position="27"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="43">
        <location>
            <begin position="205"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="43">
        <location>
            <begin position="346"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="43">
        <location>
            <begin position="418"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="42">
        <location>
            <begin position="604"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="39">
        <location>
            <begin position="1024"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="39">
        <location>
            <begin position="1044"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="39">
        <location>
            <begin position="1092"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="33">
        <location>
            <position position="830"/>
        </location>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="583"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="34">
        <location>
            <begin position="611"/>
            <end position="618"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="34 37">
        <location>
            <position position="638"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="34">
        <location>
            <begin position="686"/>
            <end position="692"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="34">
        <location>
            <position position="834"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="835"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="848"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for interaction with phosphotyrosine-binding proteins" evidence="36">
        <location>
            <position position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00029696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00032009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00066051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00075975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00075984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="RuleBase" id="RU000311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="42">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="43">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="44">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1102" mass="123966" checksum="1145660601D7D143" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GT59</accession>
    <name>A0A452GT59_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Antigen KI-67</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000005194"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006054.1">
        <property type="protein sequence ID" value="ENSGAGP00000005194.1"/>
        <property type="gene ID" value="ENSGAGG00000004220.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000006057.1">
        <property type="protein sequence ID" value="ENSGAGP00000005196.1"/>
        <property type="gene ID" value="ENSGAGG00000004220.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051983">
        <property type="term" value="P:regulation of chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007088">
        <property type="term" value="P:regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012568">
        <property type="entry name" value="KI67R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21603">
        <property type="entry name" value="ANTIGEN KI-67-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21603:SF17">
        <property type="entry name" value="PROLIFERATION MARKER PROTEIN KI-67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08065">
        <property type="entry name" value="KI67R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01295">
        <property type="entry name" value="K167R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="872"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="978"/>
            <end position="1813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="876"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="978"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="999"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1033"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1057"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1073"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1130"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1196"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1229"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1317"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1340"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1375"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1427"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1447"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1461"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1482"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1529"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1549"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1584"/>
            <end position="1594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1604"/>
            <end position="1615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1638"/>
            <end position="1652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1683"/>
            <end position="1696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1703"/>
            <end position="1714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1754"/>
            <end position="1769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1813" mass="201910" checksum="33FEB8290AA45E29" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTY8</accession>
    <name>A0A452GTY8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">THAP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>peroxynitrite = nitrate</text>
            <dbReference type="Rhea" id="RHEA:63116"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:25941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:63117"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTY8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005204"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006065.1">
        <property type="protein sequence ID" value="ENSGAGP00000005204.1"/>
        <property type="gene ID" value="ENSGAGG00000004228.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07828">
        <property type="entry name" value="lipocalin_heme-bd-THAP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045165">
        <property type="entry name" value="Nitrobindin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014878">
        <property type="entry name" value="THAP4-like_heme-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15854:SF4">
        <property type="entry name" value="PEROXYNITRITE ISOMERASE THAP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15854">
        <property type="entry name" value="THAP4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08768">
        <property type="entry name" value="THAP4_heme-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="9">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="239"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="265"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="576" mass="64616" checksum="0F753CCB752CD11B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GU11</accession>
    <name>A0A452GU11_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serum amyloid A protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Major acute phase reactant. Apolipoprotein of the HDL complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the SAA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GU11"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006426.1">
        <property type="protein sequence ID" value="ENSGAGP00000005522.1"/>
        <property type="gene ID" value="ENSGAGG00000004478.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034364">
        <property type="term" value="C:high-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.110:FF:000001">
        <property type="entry name" value="Serum amyloid A protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.110">
        <property type="entry name" value="Serum amyloid A protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000096">
        <property type="entry name" value="Serum_amyloid_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052464">
        <property type="entry name" value="Synovial_Prolif_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23424">
        <property type="entry name" value="SERUM AMYLOID A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23424:SF29">
        <property type="entry name" value="SERUM AMYLOID A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00277">
        <property type="entry name" value="SAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002472">
        <property type="entry name" value="Serum_amyloid_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00306">
        <property type="entry name" value="SERUMAMYLOID"/>
    </dbReference>
    <dbReference type="SMART" id="SM00197">
        <property type="entry name" value="SAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00992">
        <property type="entry name" value="SAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0011">Acute phase</keyword>
    <keyword evidence="2" id="KW-0345">HDL</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019347682" description="Serum amyloid A protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="88"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="127" mass="14263" checksum="61E3B3DCDA1CDC3F" modified="2019-05-08" version="1">MKFYNCILLLSLVFCVSAQNWFTNAGAFLTDAYRGAGDMLRAYSDMREANYKNADKYFHARGNYDAAQRGPGGKWAAKVISDAREGWQDGISGRGAEDTHQDQEANEWGRSGKDPNHYRPKGLPSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWN3</accession>
    <name>A0A452GWN3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. PAR subfamily.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000006426"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007480.1">
        <property type="protein sequence ID" value="ENSGAGP00000006426.1"/>
        <property type="gene ID" value="ENSGAGG00000005200.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000007484.1">
        <property type="protein sequence ID" value="ENSGAGP00000006430.1"/>
        <property type="gene ID" value="ENSGAGG00000005200.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14695">
        <property type="entry name" value="bZIP_HLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000007">
        <property type="entry name" value="hepatic leukemia factor isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF7">
        <property type="entry name" value="D SITE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="243"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="106"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="205"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="313" mass="34178" checksum="C038275405B5AEE6" modified="2019-05-08" version="1">MARPAGQLGATERLPNTLLSSAPLGVPGAGMVSLKSLLQGPIKSPERRPKDLTTCLAKEKERKMEEELGVARAGQCAYLGSLLWERTLPYSEIEYVDLDEFLLENGLPPSPAQPPFSPASQATSSPSSGIAVDLSRPASCTSSACSSPAECPGSSDGDQGCATAGPVTPASRNTPSPVDPEAVEVLMNFDPDPADLALSSIPGHETFDPRKHRFSDEELKPQPIMKKARKIXGKIQGPDEQKDEKYWNRRYKNNEAAKRSRDARRLKENQITVRAAFLEKENAVLRQEVASIRQELSRYRTILTKYESQHGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWX1</accession>
    <name>A0A452GWX1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GWX1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006596"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007670.1">
        <property type="protein sequence ID" value="ENSGAGP00000006596.1"/>
        <property type="gene ID" value="ENSGAGG00000005332.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF142">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="13"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="115" mass="13281" checksum="DE23DBE765F6539E" modified="2019-05-08" version="1">SALLPLSAKLGPVTFEEVAVYFTKEEWALLDPSQRALYRDIMQENYEMVTSLGFPIPKPDVISQLERGEEPWVLEEGTFRACFFQSSQKLLWFAPHFALSFLRFPFIPDQADIHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXG5</accession>
    <name>A0A452GXG5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dermatan-sulfate isomerase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXG5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006808"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007903.1">
        <property type="protein sequence ID" value="ENSGAGP00000006808.1"/>
        <property type="gene ID" value="ENSGAGG00000005494.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047757">
        <property type="term" value="F:chondroitin-glucuronate 5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.70:FF:000001">
        <property type="entry name" value="dermatan-sulfate epimerase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.100">
        <property type="entry name" value="Chondroitin AC/alginate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008929">
        <property type="entry name" value="Chondroitin_lyas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052447">
        <property type="entry name" value="Dermatan-Sulfate_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15532">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15532:SF2">
        <property type="entry name" value="DERMATAN-SULFATE EPIMERASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48230">
        <property type="entry name" value="Chondroitin AC/alginate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019569098" description="Sulfotransferase domain-containing protein" evidence="11">
        <location>
            <begin position="21"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="768"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="800"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="12">
        <location>
            <begin position="1019"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="949"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="968"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1216" mass="139357" checksum="E36E8E27E57DB61C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXL0</accession>
    <name>A0A452GXL0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Desmoplakin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Major high molecular weight protein of desmosomes. Regulates profibrotic gene expression in cardiomyocytes via activation of the MAPK14/p38 MAPK signaling cascade and increase in TGFB1 protein abundance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the plakin or cytolinker family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000006884"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007986.1">
        <property type="protein sequence ID" value="ENSGAGP00000006884.1"/>
        <property type="gene ID" value="ENSGAGG00000005551.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014704">
        <property type="term" value="C:intercalated disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043588">
        <property type="term" value="P:skin development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000125">
        <property type="entry name" value="Desmoplakin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000123">
        <property type="entry name" value="Desmoplakin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000102">
        <property type="entry name" value="Desmoplakin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.780:FF:000001">
        <property type="entry name" value="Plectin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000001">
        <property type="entry name" value="Plectin a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000002">
        <property type="entry name" value="Plectin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000010">
        <property type="entry name" value="plectin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1290.10">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041615">
        <property type="entry name" value="Desmoplakin_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041573">
        <property type="entry name" value="Desmoplakin_Spectrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035915">
        <property type="entry name" value="Plakin_repeat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001101">
        <property type="entry name" value="Plectin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169:SF26">
        <property type="entry name" value="DESMOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00681">
        <property type="entry name" value="Plectin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17902">
        <property type="entry name" value="SH3_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18373">
        <property type="entry name" value="Spectrin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21019">
        <property type="entry name" value="Spectrin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00250">
        <property type="entry name" value="PLEC"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75399">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="467"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1134"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="2811"/>
            <end position="2863"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="420"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="823"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="1400"/>
            <end position="1931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1139"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="2811"/>
            <end position="2822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="2823"/>
            <end position="2838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="2848"/>
            <end position="2863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2863" mass="331420" checksum="259C24278AEA3144" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXT5</accession>
    <name>A0A452GXT5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXT5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006932"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008035.1">
        <property type="protein sequence ID" value="ENSGAGP00000006932.1"/>
        <property type="gene ID" value="ENSGAGG00000005587.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000410">
        <property type="entry name" value="Zinc finger protein 777"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="122" mass="14175" checksum="A3CD6EC2C94CD639" modified="2019-05-08" version="1">MAAELGPLRIHTGERPYQCTDCEKSFTEKSKLTNHYRTHTGERPYACAQCGKRFIRKHHLLKHQRVHTGERPYQLHRCPQCEKSFRYKQSLNCHLKIHLGNHCPMGSALSPEQQTPARPLCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GY22</accession>
    <name>A0A452GY22_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calpain-2 catalytic subunit</fullName>
            <ecNumber evidence="6">3.4.22.53</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Calcium-activated neutral proteinase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Calpain M-type</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Calpain-2 large subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Millimolar-calpain</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Broad endopeptidase specificity.</text>
            <dbReference type="EC" id="3.4.22.53"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.53" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452GY22"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007016"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008128.1">
        <property type="protein sequence ID" value="ENSGAGP00000007016.1"/>
        <property type="gene ID" value="ENSGAGG00000004685.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00214">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.380:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033883">
        <property type="entry name" value="C2_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF268">
        <property type="entry name" value="CALPAIN-2 CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 23" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 23" id="KW-0645">Protease</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="14 23" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="24">
        <location>
            <begin position="45"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="25">
        <location>
            <begin position="515"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="active site" evidence="22 23">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="active site" evidence="22 23">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="active site" evidence="22 23">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00032449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00032544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00054695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="678" mass="76770" checksum="3E70394D2334ADC9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0T1</accession>
    <name>A0A452H0T1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in vesicular transport from endoplasmic reticulum to Golgi.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit transport protein particle (TRAPP) complex. The heterodimer TRAPPC6B-TRAPPC3 interacts with TRAPPC1 likely providing a core for TRAPP complex formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Golgi apparatus</location>
            <location evidence="8">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAPP small subunits family. BET5 subfamily.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000008048"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009271.1">
        <property type="protein sequence ID" value="ENSGAGP00000008048.1"/>
        <property type="gene ID" value="ENSGAGG00000006400.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14855">
        <property type="entry name" value="TRAPPC1_MUM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.70:FF:000004">
        <property type="entry name" value="Trafficking protein particle complex 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF16">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="194"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="225" mass="25558" checksum="B20A069ADA5CADE1" modified="2019-05-08" version="1">MTVHNLYLFDRNGICLHYGEWHRKKQAGISMEEEFKLMYGMLFSMRSFVGKMSPVDMKDGFLAFQTSKYKLHYYETPSGLKVVMNTDLGVGNIRDVLHQIYSNIYVEFVVKNPLCSLSEPIQSELFRAKLDGFIRGLPFFSARAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSWHPPRLSTRLCRPQPSGQDTGLPLAHLHPPGPFTAQTAWPTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H100</accession>
    <name>A0A452H100_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the NOD1-NOD2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H100"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008131"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009364.1">
        <property type="protein sequence ID" value="ENSGAGP00000008131.1"/>
        <property type="gene ID" value="ENSGAGG00000006465.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000009368.1">
        <property type="protein sequence ID" value="ENSGAGP00000008134.1"/>
        <property type="gene ID" value="ENSGAGG00000006465.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000009369.1">
        <property type="protein sequence ID" value="ENSGAGP00000008135.1"/>
        <property type="gene ID" value="ENSGAGG00000006465.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08324">
        <property type="entry name" value="CARD_NOD1_CARD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001074">
        <property type="entry name" value="Nucleotide binding oligomerization domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000047">
        <property type="entry name" value="Nucleotide-binding oligomerization domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000282">
        <property type="entry name" value="Nucleotide-binding oligomerization domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000666">
        <property type="entry name" value="Nucleotide-binding oligomerization domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="CARD" evidence="17">
        <location>
            <begin position="26"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="18">
        <location>
            <begin position="201"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="951" mass="108852" checksum="65AC77C2CD6283E9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1U3</accession>
    <name>A0A452H1U3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H1U3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008598"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009887.1">
        <property type="protein sequence ID" value="ENSGAGP00000008598.1"/>
        <property type="gene ID" value="ENSGAGG00000006815.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000009890.1">
        <property type="protein sequence ID" value="ENSGAGP00000008601.1"/>
        <property type="gene ID" value="ENSGAGG00000006815.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09498">
        <property type="entry name" value="SAM_caskin1_2_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000032">
        <property type="entry name" value="caskin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033635">
        <property type="entry name" value="ANKS1/Caskin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035498">
        <property type="entry name" value="Caskin1/2_SAM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032117">
        <property type="entry name" value="Caskin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174">
        <property type="entry name" value="ANKYRIN REPEAT AND STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174:SF18">
        <property type="entry name" value="CASKIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16907">
        <property type="entry name" value="Caskin-Pro-rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16632">
        <property type="entry name" value="Caskin-tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="5">
        <location>
            <begin position="36"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="166"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="328"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="411"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="908"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="166"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="423"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="476"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="576"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="629"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="667"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="677"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="967" mass="103760" checksum="9AA5E00A5D147FEB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1V5</accession>
    <name>A0A452H1V5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H1V5"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009821.1">
        <property type="protein sequence ID" value="ENSGAGP00000008534.1"/>
        <property type="gene ID" value="ENSGAGG00000006778.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000009822.1">
        <property type="protein sequence ID" value="ENSGAGP00000008535.1"/>
        <property type="gene ID" value="ENSGAGG00000006778.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000009824.1">
        <property type="protein sequence ID" value="ENSGAGP00000008537.1"/>
        <property type="gene ID" value="ENSGAGG00000006778.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029266">
        <property type="entry name" value="FAM217"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22145:SF4">
        <property type="entry name" value="PROTEIN FAM217A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22145">
        <property type="entry name" value="SI:CH211-266K22.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15344">
        <property type="entry name" value="FAM217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="424" mass="48334" checksum="1005E2F62D50645F" modified="2019-05-08" version="1">MFHSWRYTHDQSNSTVNRDSNKPLVEKLSSSHGNSLNFFTVNPALVGTHMNSYHTVSHRGPRQMPLGIYWTNGDGDFFKARNETTASDSYPATESSSDDTFCMSKWNLKLRNSRVENSLLTKEGDVSESEKANDVLLSYFKYMDLNLRPETIENIEKASSKHQNEVFPYPDFLPPPFNNLDLQKLALSKWDDWKEVFNPPPESSVVKLISRLLEIERMQHLTILKEKTRDLTTSSSMMLGSRPSSTKSMHQLKQSKLSDPLCLQTPFNRDYQEKNKSTHSGCCTHEWNSSKCTWEYCHHCKWSAGTSSIRSSSTKQLRASCDAFKSSKTPIILNSNMLLRRSSSCCVAAPRTQSTLKLTSPKLLPPSTAITSPFPDKDSLKHKQSRTKKKLYRKNVVTGKTFHCQKLKSVSAMPKQKYTHTVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3I6</accession>
    <name>A0A452H3I6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H3I6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009124"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010473.1">
        <property type="protein sequence ID" value="ENSGAGP00000009124.1"/>
        <property type="gene ID" value="ENSGAGG00000007206.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045323">
        <property type="entry name" value="CCDC34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025259">
        <property type="entry name" value="CCDC34/181"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23247:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23247">
        <property type="entry name" value="NY-REN-41 ANTIGEN L15 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13904">
        <property type="entry name" value="CCDC34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coiled-coil" evidence="2">
        <location>
            <begin position="96"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="327" mass="38852" checksum="5409FC7AB6EA0A79" modified="2019-05-08" version="1">MLSARPRSRRRRVACSRPPSPTSSEDEQPLSSSTYSLLSPTYHQSFRSCSVEEDNREETALSAREQRTFKRNKHNSNAESCNVTEKKKQSSLADNLSPWEEWFICKERELRVRLQARALEEMNLQLEKRKEKQELERKKKIAEEKHKEWVQKKNEEERREKERKLIKKMAEKAARDLERVQLQEKAKGKYKEWLKKKRAEESEKKKKEEEKEQKRIAELQEKKEKSEKIFREWLQNARNKPRPVLHGYGYTNGKLSGYPGRNSYPAPTYCNPIPWKPIHVPPPKEDKNVTVKKNKRPISSQSYRSSSMVIHKPKNNLCIGSLRRKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H492</accession>
    <name>A0A452H492_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H492"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009479"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010888.1">
        <property type="protein sequence ID" value="ENSGAGP00000009479.1"/>
        <property type="gene ID" value="ENSGAGG00000007495.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF117">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="12">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="13">
        <location>
            <begin position="132"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="211"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="318" mass="35652" checksum="5420795A285064ED" modified="2019-05-08" version="1">MSWSFLTRLLEEINNHSTFVGKVWLTVLIVFRIVLTAVGGESIYYDEQSKFVCNTQQPGCENVCYDAFAPLSHVRFWIFQIIMVATPSVMYLGFGGRMPVVRRGEKHDGRRRIRQDGLMKAYVLQLLCRSVLEVAFLFGQYVLYHFEVSPSYVCNRSPCPHIVDCFVSRPTEKTIFLLIMYAVSGLCLFLNLLELCHLGVGRIRDLLHQGSDSPSLHDSHSGPHYAKRAPSAPPTYHSLKKEPPKGHLKHLRPSPLGPAASPPPPAVRDAALPWQRACVQSHGGGRFPTVAGSCRRVGIPRGWAYAHHSLEVLPPSWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4S3</accession>
    <name>A0A452H4S3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H4S3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009506"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010922.1">
        <property type="protein sequence ID" value="ENSGAGP00000009506.1"/>
        <property type="gene ID" value="ENSGAGG00000007508.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF9">
        <property type="entry name" value="AFG3-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="150"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="571"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="585"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="620" mass="68848" checksum="A3BE70664DB3352E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4W7</accession>
    <name>A0A452H4W7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H4W7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009547"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010974.1">
        <property type="protein sequence ID" value="ENSGAGP00000009547.1"/>
        <property type="gene ID" value="ENSGAGG00000007548.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17982">
        <property type="entry name" value="DEXHc_DHX37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000014">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DHX37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000895">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056371">
        <property type="entry name" value="DHX37-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF99">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23362">
        <property type="entry name" value="DHX37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="256"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="499"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="131"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="492"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="537"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="131"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="495"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="537"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1145" mass="129073" checksum="FA05D01F1CC65D13" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H589</accession>
    <name>A0A452H589_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H589"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009669"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011106.1">
        <property type="protein sequence ID" value="ENSGAGP00000009669.1"/>
        <property type="gene ID" value="ENSGAGG00000007630.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000029">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF51">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="349"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="8"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="349"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="422" mass="43682" checksum="509439A6D96251A2" modified="2019-05-08" version="1">KAAIRPRGLSGNPAAGSAAGGASSNRRLGRGLRSAGLSSAATGRKPHPASQLGTKQHPAGALAKGGIQPRTAPALQGALPHQPQHRIAAPKASSAEGATMALLKLSLMAFSFVFWAAGLTMLTLGIWAKISLGSYLVLSANQYPNTPFILLATGAAVLVWGFLGCFSAATEHRCLLRTYAAFQLAVLAAGLAAGLSALFYRRDIAEGFRAGLREAVRAYGENEQKADALDSLQRALDCCGAESYRDWLASPWSLAQPARNGSVPGSCCRARRGCQHSPLQPEARGIHRDGCFAKVSGFVSDNLFYIATAALGLALLQGVGIVLACLLAARLRPAPSAQPGCSQRPALIARSQRPAPSAPSARLRPPAAPGRGIASPRARPAAAPGPGCSLRPRPRLLPRDSLFGARAPPIAAAVRPAAPPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9R2</accession>
    <name>A0A452H9R2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H9R2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011331"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012975.1">
        <property type="protein sequence ID" value="ENSGAGP00000011331.1"/>
        <property type="gene ID" value="ENSGAGG00000008768.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15221">
        <property type="entry name" value="7tmA_OR52B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000006">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF32">
        <property type="entry name" value="OLFACTORY RECEPTOR 52B6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="43"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="319" mass="35857" checksum="8FFA3A23F68BBF62" modified="2019-05-08" version="1">MPADNHTFFAPMTYILTGIPGTKESHVWISILLCLMYVVALFGNSLLLFIIVTERSRHEPMYLFVSMLAAADLLLSTTAVPKMLAVFWFRAGEISFAACLTQLFFIHVSFMAESGILLAMAFDRYVAICDPLRYTIILTKSVMRKMGLAVVTRSFCIIFPLIFLVKMKFCGTNLPPHTYCEHLAIAWLACEDITVSIWYGLAVAIFVIGLDTVLIAVSYGLILRAVFLLPSKDARLKALHTCSSHLCVILIFYTPAFFPIFAHRFGHIIPGYILSLLTNLYVLIPPMLNPIVYGVTTKEILKRVINVFHRWCRGSSLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAH7</accession>
    <name>A0A452HAH7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HAH7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011738"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013444.1">
        <property type="protein sequence ID" value="ENSGAGP00000011738.1"/>
        <property type="gene ID" value="ENSGAGG00000009058.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21977">
        <property type="entry name" value="HMG-box_BHMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47658">
        <property type="entry name" value="HIGH MOBILITY GROUP B PROTEIN 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47658:SF1">
        <property type="entry name" value="MEIOSIS INITIATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="3">
        <location>
            <begin position="428"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="1">
        <location>
            <begin position="428"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="62"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="368"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="370"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="526" mass="57974" checksum="15EB70DD8CAB60B5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAT5</accession>
    <name>A0A452HAT5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HAT5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011845"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013564.1">
        <property type="protein sequence ID" value="ENSGAGP00000011845.1"/>
        <property type="gene ID" value="ENSGAGG00000009128.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF142">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="24"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="153" mass="17206" checksum="8A4E77EC5B9B994F" modified="2019-05-08" version="1">MTPRSVSAVLLPRPVGLLLFQGPVTFEEVAVYFTREEAALLDPTQRALYRDVMQENYETVVLLGFPISKPDVILQLERGEEPWVLDLQGSEKEVLPRAAFTGEELVKQLNNCLGMQETFGMPYKDPVSSSSSGFFPADVESLWQMSLMTSLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBK8</accession>
    <name>A0A452HBK8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452HBK8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012146"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013912.1">
        <property type="protein sequence ID" value="ENSGAGP00000012146.1"/>
        <property type="gene ID" value="ENSGAGG00000009348.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072518">
        <property type="term" value="F:Rho-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901888">
        <property type="term" value="P:regulation of cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000047">
        <property type="entry name" value="Rho-associated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000072">
        <property type="entry name" value="Rho-associated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001759">
        <property type="entry name" value="Rho-associated, coiled-coil-containing protein kinase 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF33">
        <property type="entry name" value="RHO-ASSOCIATED PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17 22 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="21" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 22 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 23" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="72"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="25">
        <location>
            <begin position="337"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="400" mass="46536" checksum="CBA98D0B560F7BBE" modified="2019-05-08" version="1">LFRPPPKKKTQKKPKPPKKHFWEKYFCWYDGLDALVYDLDFPALRKNKNIDNFLNRYTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSSRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKAGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGQECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKDAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HC76</accession>
    <name>A0A452HC76_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Aspartate aminotransferase</fullName>
            <ecNumber evidence="14">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2S)-2-aminobutanoate + 2-oxoglutarate = 2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:70223"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:70225"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3-sulfino-L-alanine + 2-oxoglutarate = 3-sulfinopyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:70295"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="ChEBI" id="CHEBI:140699"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:70297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:21825"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-cysteine + 2-oxoglutarate = 2-oxo-3-sulfanylpropanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:17441"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57678"/>
            <dbReference type="EC" id="2.6.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:17442"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="14">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A452HC76"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012385"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014176.1">
        <property type="protein sequence ID" value="ENSGAGP00000012385.1"/>
        <property type="gene ID" value="ENSGAGG00000009513.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120554">
        <property type="term" value="F:2-aminobutanoate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047801">
        <property type="term" value="F:L-cysteine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000044">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF3">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 14" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="15">
        <location>
            <begin position="32"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="413" mass="46254" checksum="922DE028FFDDA1DD" modified="2019-05-08" version="1">MAAGSLFASVPRAPPVAVFQLTADFREDRDPRKVNLGVGAYRTDEGQPWVLPVVKKVEQKIANDASLNHEYLPILGLPEFRANASRIVLGDDSRAIKENRVGGVQCLGGTGGLRIGAEFLRRWYNGTNNTATPVYISSPSWENHNSVFVNAGFKDIRAYRYWDAAKRGLDLEGLLEDMESAPEFSIFVLHACAHNPTGTDPTLEQWKQIAAVMKRRFLFPFFDSAYQGFASGCLDRDAWAVRYFVSEGFELFCAQSFSKNFGLYNERVGNLSVVGKDADNVQRVLSQMEKIVRTIWSNPPSQGARIVATTLTCPQLFAEWKDNVKTMADRVLLMRSELRARLQALGTPGTWNHITEQIGMFSFTGLNPKQVEYMIKEKHIYLMASGRINMCGLTTKNLDYVASSIHEAVTKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDC1</accession>
    <name>A0A452HDC1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HDC1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012818"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014692.1">
        <property type="protein sequence ID" value="ENSGAGP00000012818.1"/>
        <property type="gene ID" value="ENSGAGG00000009839.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019480762" evidence="12">
        <location>
            <begin position="26"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="193"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="245" mass="26518" checksum="03FFC2D69337298C" modified="2019-05-08" version="1">MRTPSVIIVGLVLGPCGLLLNLVSTLTPCWRQVSSISGKPIDVVYQQGIWELCQETQSTRQRQCGLADELGYFPTQPVPVAKGLMVSSLVVTVLGLVVAALGVRCWQEEPRYLLAGLSGLILFASGLMSLIPVSWYNHDLSALPAPAGSTLEVGYSLVLGYLGSCLELIGGFSLALSLHQYCQECRRKKTTTKYLHHSQPGPATVSSRDFMPRSQKPAPKSFSNPMDVLEGERSVSHSLPCDSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEA1</accession>
    <name>A0A452HEA1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase RNF182</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">RING finger protein 182</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase RNF182</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Interacts with ATP6V0C.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HEA1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013151"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015056.1">
        <property type="protein sequence ID" value="ENSGAGP00000013151.1"/>
        <property type="gene ID" value="ENSGAGG00000010080.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042285">
        <property type="entry name" value="RNF182"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46675">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46675:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="20"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="242" mass="27318" checksum="AF7FA855F6D1A2D8" modified="2019-05-08" version="1">MMSYPHTEGEEKVPNDELECKICYLRFNIHSRKPKILNCLHRVCARCLTKILHIGDGCPCISCPFCRHETQLHEDEVEGLPNDTNVMSKLMLKDKTIWNSDYKEVVLTPKNLAFSSPSHGSSNCLVITIMEVQRDSTRTPSQNTISDYYADHSLDSVSVSSHSQLDQDLFSKLCNHVPRILVWLLGFFYFGSLPLGIYLLVIQKVTLGIVCVSLVPSSLTVCLVYGFCQCLWQGMCDCSSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HF52</accession>
    <name>A0A452HF52_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Proliferation-associated SNF2-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HF52"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013456"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015400.1">
        <property type="protein sequence ID" value="ENSGAGP00000013456.1"/>
        <property type="gene ID" value="ENSGAGG00000010269.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006346">
        <property type="term" value="P:DNA methylation-dependent constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046651">
        <property type="term" value="P:lymphocyte proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18009">
        <property type="entry name" value="DEXHc_HELLS_SMARCA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000015">
        <property type="entry name" value="lymphoid-specific helicase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000577">
        <property type="entry name" value="lymphoid-specific helicase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044753">
        <property type="entry name" value="HELLS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47161">
        <property type="entry name" value="LYMPHOID-SPECIFIC HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47161:SF1">
        <property type="entry name" value="LYMPHOID-SPECIFIC HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="98"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="467"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00053349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="701" mass="81151" checksum="7FD166F259091C9A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFE6</accession>
    <name>A0A452HFE6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Olfactomedin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFE6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013561"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015522.1">
        <property type="protein sequence ID" value="ENSGAGP00000013561.1"/>
        <property type="gene ID" value="ENSGAGG00000010355.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF29">
        <property type="entry name" value="OLFACTOMEDIN-LIKE PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Olfactomedin-like" evidence="5">
        <location>
            <begin position="355"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="175"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="263"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="180"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="306"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="612" mass="69695" checksum="8C00F5381AD827E9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HH22</accession>
    <name>A0A452HH22_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LRP2-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May act as an adapter that regulates LRP2 function.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HH22"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014173"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016222.1">
        <property type="protein sequence ID" value="ENSGAGP00000014173.1"/>
        <property type="gene ID" value="ENSGAGG00000010786.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052323">
        <property type="entry name" value="LRP2-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44554">
        <property type="entry name" value="LRP2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44554:SF1">
        <property type="entry name" value="LRP2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="70"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="355" mass="40064" checksum="93C25EFFE2B14F7E" modified="2019-05-08" version="1">MCYQSAKFTFSFFFTSMPQSGVESFSLHLKEKKMYRPHDCRGKHAGNYSHTALFARAEELLAKRIIDGDPLAYFLQGQLYFEEGWYDEALAQFEKIKEMDFQALYQLGVMYYDGLGTKEDPEKGVEYMKIIINSNSPKARHLKYAAAYNLGRSYFEGCGVKHSDKEAERLWIIAADHGNPKASVKAQSTLGMFYSTSNPKDLKKAFFWHSEACGNGSLESQGILGVMYLYGQGIRQNTKAALECLKEAAERGNIYAQGHLVEYYYKRKFYTKAAAFARRIAENDNIDMLAKITDCLPVYIAKGVAMATFYLARCLQLGVGIQQDQAAAKNYYSRACLLDPDVASDLELAANHGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HH75</accession>
    <name>A0A452HH75_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 39 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HH75"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014219"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016270.1">
        <property type="protein sequence ID" value="ENSGAGP00000014219.1"/>
        <property type="gene ID" value="ENSGAGG00000010818.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003940">
        <property type="term" value="F:L-iduronidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000059">
        <property type="entry name" value="Alpha-L-iduronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1500:FF:000002">
        <property type="entry name" value="Iduronidase alpha-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1500">
        <property type="entry name" value="Glycosyl hydrolase domain, family 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049165">
        <property type="entry name" value="GH39_as"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049167">
        <property type="entry name" value="GH39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049166">
        <property type="entry name" value="GH39_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000514">
        <property type="entry name" value="Glyco_hydro_39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051923">
        <property type="entry name" value="Glycosyl_Hydrolase_39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12631">
        <property type="entry name" value="ALPHA-L-IDURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12631:SF8">
        <property type="entry name" value="ALPHA-L-IDURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01229">
        <property type="entry name" value="Glyco_hydro_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21200">
        <property type="entry name" value="Glyco_hydro_39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00745">
        <property type="entry name" value="GLHYDRLASE39"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01027">
        <property type="entry name" value="GLYCOSYL_HYDROL_F39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019250544" evidence="5">
        <location>
            <begin position="24"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolases family 39 N-terminal catalytic" evidence="6">
        <location>
            <begin position="27"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-L-iduronidase C-terminal" evidence="7">
        <location>
            <begin position="546"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600514-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="626" mass="70709" checksum="8CEF200F5B902084" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HH86</accession>
    <name>A0A452HH86_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HH86"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014233"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016291.1">
        <property type="protein sequence ID" value="ENSGAGP00000014233.1"/>
        <property type="gene ID" value="ENSGAGG00000010835.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15225">
        <property type="entry name" value="7tmA_OR10A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="211"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="55"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="326" mass="37116" checksum="015777C411804E30" modified="2019-05-08" version="1">NFPLLYIRMKTRQETVGRNSTTITEFILLGFSDIPSLQHLFFSVFLVTYIVTLAGNLLIIVLTLADPALHTPMYFFLRNLSFLEMCYASVNVPKMLRNLLSWDKTISFIGCAMQTYFIFFLGGSECFLLASMAYSRYVAICKPLHYPVVMNRKVSTGLVVGSWLSGLLVYFGLTSMVFILPFCRSHEINHFFYDIPPVLKLACGDTSRNEMVVFMVALFFVTFPFMLILMSYVCIISTILRVPSGAGRRKSFSTCSSHLMVVTLFFGSVCIMYLKPNSSYSPNTDKFLSLSYTVITPMLNPIIYSLRNKEVKGAFWRMKVSLIKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HH96</accession>
    <name>A0A452HH96_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HH96"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014257"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016316.1">
        <property type="protein sequence ID" value="ENSGAGP00000014257.1"/>
        <property type="gene ID" value="ENSGAGG00000010851.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019585375" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="19548" checksum="6C6C446CE4A5EC58" modified="2019-05-08" version="1">RAGGLLWLLLAWGPHSSLDTSLLESGGDVKKPGDSLRLTCTTAGFNIDSYWMHWVRQAPGQGLEWVSAILGATIYYRTGLSSDKNLPLWSAGEVPAGLTLTVRALGSHRRQQCVPLEMVKMYSALQPSLGPHTAEISSSAIVQLPTNLFSSPPHLPRTCPSITGGFLETLDSLTFHYKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIC0</accession>
    <name>A0A452HIC0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HPF1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HIC0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014651"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016753.1">
        <property type="protein sequence ID" value="ENSGAGP00000014651.1"/>
        <property type="gene ID" value="ENSGAGG00000011120.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072572">
        <property type="term" value="F:poly-ADP-D-ribose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019361">
        <property type="entry name" value="HPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13386">
        <property type="entry name" value="HISTONE PARYLATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13386:SF1">
        <property type="entry name" value="HISTONE PARYLATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10228">
        <property type="entry name" value="HPF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="358" mass="40711" checksum="4C5E9B478CFC225F" modified="2019-05-08" version="1">MPPEVPAGRGAAGRNMTGGGKRRARAGEQCENTKEVKKRRSNQADVPDQLRQEVESCYRLRMPEDFYQFWKFCEELKPDRPSEALLSSIGLQLVGPYDILAGKHKKTKSANLNFNLHWRFFYDPPEFQTIIAGDSKTQYHMGYFRDVPDELPAWVGANKAKKNCVISQIGDNVFAAVKLFLSKKLKEVTDKKKNGILKDIDENLTRTAKELGYSLEQKTMKMKQRDKKVVTKTFHGAGLVVPVDKNDVGYRELPETNANLKRICKAIVDAPSDDERLKAFAPIQEMITFVQFANDECDYGMGYELGMDLFCYGSHYFHKVVGQLLPLAYNLLRRNLFAEIVESHLANRSEENVDQLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIE4</accession>
    <name>A0A452HIE4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000014676"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016781.1">
        <property type="protein sequence ID" value="ENSGAGP00000014676.1"/>
        <property type="gene ID" value="ENSGAGG00000011140.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071723">
        <property type="term" value="F:lipopeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035663">
        <property type="term" value="F:Toll-like receptor 2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071221">
        <property type="term" value="P:cellular response to bacterial lipopeptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002224">
        <property type="term" value="P:toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001359">
        <property type="entry name" value="Toll-like receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000001">
        <property type="entry name" value="Toll-like receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF422">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019310935" description="TIR domain-containing protein" evidence="15">
        <location>
            <begin position="24"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="16">
        <location>
            <begin position="515"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="673" mass="77854" checksum="2C6802F8B00DEE52" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIM3</accession>
    <name>A0A452HIM3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm protein F</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HIM3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014719"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016827.1">
        <property type="protein sequence ID" value="ENSGAGP00000014719.1"/>
        <property type="gene ID" value="ENSGAGG00000011163.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01722">
        <property type="entry name" value="Sm_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034100">
        <property type="entry name" value="Sm_F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="57" mass="6461" checksum="B8CC40D2FA255CD5" modified="2019-05-08" version="1">MEYKGYLVSADGYMNMQLANTEEYIDGALSGHLGEVLIRCNNVLYLRGVEEDGEMRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJY3</accession>
    <name>A0A452HJY3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HJY3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015227"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017392.1">
        <property type="protein sequence ID" value="ENSGAGP00000015227.1"/>
        <property type="gene ID" value="ENSGAGG00000011492.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042269">
        <property type="term" value="P:regulation of natural killer cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051527">
        <property type="entry name" value="KLR_subfamily_B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46784">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR SUBFAMILY B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46784:SF1">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR SUBFAMILY B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="55"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="160" mass="17969" checksum="CD39B5566C66046A" modified="2019-05-08" version="1">RVLSQHAITLTECHHFPHWHQMALRVSGAANVILLGAMMALSIWGELCAPNWLLHGDKCYWLSKESKKWNESREDCSAKSSQMLMSQDQREMAFIKTITEGKYSIWIGLHLTTSKGNWTWVDGSLLESPCNLIIVAVRGAKCKALLGWLAKVTFTPGGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HL33</accession>
    <name>A0A452HL33_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Galanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galanin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HL33"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015631"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017851.1">
        <property type="protein sequence ID" value="ENSGAGP00000015631.1"/>
        <property type="gene ID" value="ENSGAGG00000011787.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031763">
        <property type="term" value="F:galanin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008174">
        <property type="entry name" value="Galanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008175">
        <property type="entry name" value="Galanin_pre"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16839">
        <property type="entry name" value="GALANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16839:SF1">
        <property type="entry name" value="GALANIN PEPTIDES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01296">
        <property type="entry name" value="Galanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00071">
        <property type="entry name" value="Galanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00861">
        <property type="entry name" value="GALANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0372">Hormone</keyword>
    <keyword evidence="5" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019153602" description="Galanin domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Galanin" evidence="7">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="113" mass="11954" checksum="65330406E7A75512" modified="2019-05-08" version="1">MGTPAVLCISLILWGLLGECAGIPLMLKDKRGWTLNSAGYLLGPHAHRPLMDKGALAGKREAAEEPVQLEVEFDGAPLRPPAERGLQTLLDFFSSLCLPGECQGRARGGEGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLY9</accession>
    <name>A0A452HLY9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mini-chromosome maintenance complex-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCMBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HLY9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015997"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018277.1">
        <property type="protein sequence ID" value="ENSGAGP00000015997.1"/>
        <property type="gene ID" value="ENSGAGG00000012029.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019140">
        <property type="entry name" value="MCM_complex-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13489">
        <property type="entry name" value="MINI-CHROMOSOME MAINTENANCE COMPLEX-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13489:SF0">
        <property type="entry name" value="MINI-CHROMOSOME MAINTENANCE COMPLEX-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09739">
        <property type="entry name" value="MCM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="198" mass="22984" checksum="6B0A782DA9071C92" modified="2019-05-08" version="1">MPCVADWLNSPLSIVQGIFAQNGPSPDWEKKVTEYFTEKLKENNATNWVPSLNDVPLHYLKPNSLVKFRCMVQDMFDPEFYMGVYETIDPHTKTRVLHFGKYRDVAEYGPQQEMDLNSSQMVTSERQTFYCVPVPGESAWLALQHPTLQVATRGVMRKMKIWSCIHLNRKSSIWVTFDILITGHFITIMNYLCIRVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMK6</accession>
    <name>A0A452HMK6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4657 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HMK6"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018472.1">
        <property type="protein sequence ID" value="ENSGAGP00000016178.1"/>
        <property type="gene ID" value="ENSGAGG00000012142.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027958">
        <property type="entry name" value="DUF4657"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37336">
        <property type="entry name" value="SIMILAR TO 9930012K11RIK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37336:SF1">
        <property type="entry name" value="SIMILAR TO 9930012K11RIK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15552">
        <property type="entry name" value="DUF4657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4657" evidence="2">
        <location>
            <begin position="154"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="474" mass="52401" checksum="91136641F768B1C1" modified="2019-05-08" version="1">MLRRRRVFAVEPLWSRDGARKPWESCVVLTSASVYRKLQEASGESTDPAPEPQRGSRMSGELGLWGPAGSPTEPGLPHQLGLFPKSGRLLKSESEDSGVEMAGNENSPSTPLGSESSFSLDCLDGFQPSTADSAASDPEEGCPEGEGPEEPDPLRERAYLRNLSVSKKLAQVLQRSRKLHAPSRSPKLLGRKSLSLVELELLASYGLQGLSGKEGAAEGHSQALSSHRSLEEQITESCGEEAEEEVRRDLSLTLPGQGLRYLEHICLMLEKIAQLQQANLQLQQQQKVMENQLWGQQAENAEPSEERPCRAPEEQGLSQPEPENRAEVEMQEEDLPLDSWRPHPFRARLASDTGVLQDLTRSSENEPVCSRKTAMHCVSSPTLLDQPDWGAHTLPPNMRPRNDRSHWGKVKVLINRITRKSIRASEPSLFGESAVDSRHCSLEASLEKQGSHPRRSFLPALGVKKHHSKTLSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNC5</accession>
    <name>A0A452HNC5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HNC5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016480"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018809.1">
        <property type="protein sequence ID" value="ENSGAGP00000016480.1"/>
        <property type="gene ID" value="ENSGAGG00000012330.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="16"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="184" mass="20079" checksum="A3BB5C983AFAD28A" modified="2025-10-08" version="2">MSMPFLYIYLHFIYNPGSYGQVTMTQSPEPLSVSPGEKVTIRCTASSSISSSYVSWYQQKPGQAPKFLIYQASYCASGVPARFSGSGAGTDYTLSISSVEANDAADYYCFQWSSSPPTVIQTNTKTSLVWVFANADFCEDNHTVSVTDAPLHLQFRLCQQDQSAVCLGVTSSAIIIDIKSQPCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNE2</accession>
    <name>A0A452HNE2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HNE2"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018799.1">
        <property type="protein sequence ID" value="ENSGAGP00000016471.1"/>
        <property type="gene ID" value="ENSGAGG00000012324.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001230">
        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="14"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="134" mass="15104" checksum="7DF85747DCBE81F6" modified="2019-05-08" version="1">MILNILYYMINFIPLSNDVTMTQSPESLSVFPGEKVTIRCTASSTLSSYAVSWYQQKPRQAPKLLIYQDSDRPYGVPARFSGSRSGTIYTLAISSVEANEAADYYCLQTQHSPLTVIQTKTKTSPLWESECRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HPN8</accession>
    <name>A0A452HPN8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutamate dehydrogenase 1, mitochondrial</fullName>
            <ecNumber evidence="3">1.4.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-glutamate + NADP(+) + H2O = 2-oxoglutarate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.3" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452HPN8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016969"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019367.1">
        <property type="protein sequence ID" value="ENSGAGP00000016969.1"/>
        <property type="gene ID" value="ENSGAGG00000012669.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000100">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="267"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="509" mass="54987" checksum="09451229AC20F767" modified="2019-05-08" version="1">MAPPHSPSLLGAPAHCPSLLGAPAHCPMAPPHSPSLLGAPAHCPMAPPPGLAPPLCWRRGKHVAVAAQAGLDAPGLLPMVETFCARAMRVAEADLVQQLRSVRPAKEKRQWVHGILETIQRCSHVLEVSIPVKRDSGHWEVIEGWRAQHSQHLLPCKGGVRFCESIGIDDVKALAALMTYKCAVVGKSVRAGKERELEKITRQFTVEMAKKGFIGPGTDVAAPDINTGEREMSWIADTYSRTLGYKDINAHACVTGKPISQGGIHGRVPATGRGLLHGIENYINNTLYMNLIGLSPGFPGKTFVLQGFGNTGLHTMRYLHRCGACCVCVGEVDGAIYNSSGIHPKELQDYKTEHGTIVGFPKAEPYEGSVLEVPCDILIPAAVEKQLTKENAHRVQAKIIAEAANGPTTPAAHEIFLQRNILVIPDLYVNAGGVTVSFFEWLKNLNHVSYGRLTFKYERDSNYHLLMSVQQSLERTLGKGSGEIPIVPSPEFQARVAVSPGLSWVAGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HPW9</accession>
    <name>A0A452HPW9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bestrophin homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Forms chloride channels.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the anion channel-forming bestrophin (TC 1.A.46) family. Calcium-sensitive chloride channel subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HPW9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017061"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019469.1">
        <property type="protein sequence ID" value="ENSGAGP00000017061.1"/>
        <property type="gene ID" value="ENSGAGG00000012736.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000615">
        <property type="entry name" value="Bestrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021134">
        <property type="entry name" value="Bestrophin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10736">
        <property type="entry name" value="BESTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10736:SF1">
        <property type="entry name" value="BESTROPHIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01062">
        <property type="entry name" value="Bestrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0868">Chloride</keyword>
    <keyword evidence="7" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="514" mass="58678" checksum="AACD6F5E513DA0C9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQ58</accession>
    <name>A0A452HQ58_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Solute carrier family 15 member 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Proton oligopeptide cotransporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQ58"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017156"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019580.1">
        <property type="protein sequence ID" value="ENSGAGP00000017156.1"/>
        <property type="gene ID" value="ENSGAGG00000012809.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17349">
        <property type="entry name" value="MFS_SLC15A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000316">
        <property type="entry name" value="Solute carrier family 15, member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="488"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="557" mass="63147" checksum="D555DDA43AACE3E4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQB3</accession>
    <name>A0A452HQB3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the EGR C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQB3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017203"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019630.1">
        <property type="protein sequence ID" value="ENSGAGP00000017203.1"/>
        <property type="gene ID" value="ENSGAGG00000012830.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000092">
        <property type="entry name" value="Early growth response protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021849">
        <property type="entry name" value="EGR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF60">
        <property type="entry name" value="STRIPE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11928">
        <property type="entry name" value="DUF3446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="323"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="381"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="295"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="394"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="398"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="412"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="441"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="454" mass="47982" checksum="BBC80CEEC0520A60" modified="2019-05-08" version="1">MMTAKTVDKIPVTLSGFVHQLSENIYPVDDIATTLPTSVTIFPNADLGGPFDQMSSVTGDGMINVDMSDKRSLDLPYASSFAPAVSASRNQTFTYMGKFSIDPQYPGAGCYPEGIINIVSAGILQGVSTPSSSATSSSTASSASPNPLASSALSCSMAQNQPGDLEHLYSPPPPYSGCGELYQQDPSSAFLPAAAGGSLPYHPPPSYPSPKAAADGGIFSMIPDYPGFFPPAQCQRELHAPPERKPFPCPLDSLRVPPPLTPLSTIRNFTLGGPGAGAAGGSGEGGRLPASAYSPHNLPLRPILRPRKYPNRPSKTPVHERPYPCPAEGCDRRFSRSDELTRHIRIHTGHKPFQCRICMRNFSRSDHLTTHIRTHTGEKPFACDFCGRKFARSDERKRHTKIHLRQKERKGAAAPSSSSGASAPAAPPPGAVCGGSGAAGSGSLAACASRTRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQJ3</accession>
    <name>A0A452HQJ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Fibronectin type III and SPRY domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">May be involved in microtubule organization and stabilization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Oligomerization is required for binding to microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQJ3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017296"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019737.1">
        <property type="protein sequence ID" value="ENSGAGP00000017296.1"/>
        <property type="gene ID" value="ENSGAGG00000012879.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12901">
        <property type="entry name" value="SPRY_PRY_FSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000566">
        <property type="entry name" value="fibronectin type III and SPRY domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017903">
        <property type="entry name" value="COS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035742">
        <property type="entry name" value="SPRY/PRY_FSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF4">
        <property type="entry name" value="FIBRONECTIN TYPE III AND SPRY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51262">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COS" evidence="18">
        <location>
            <begin position="105"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="164"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="16">
        <location>
            <begin position="268"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="303"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="496" mass="56175" checksum="13ACC00B725A00C2" modified="2019-05-08" version="1">MGDQKDALRKIITTLAMKNEEIQNFIYALKQMLQNVEANSTKVQEDLEGEFQSLYSLLDELKEGMLMKIKQDRASRTYELQNQLAACTKALESSEELLEMANQTLEGAENHDFNQAAKQIKDSVTMAPAFRLSLKAKVSDNMSHLMVDFTQERHMLQSLKFLPVPSAPEIDMAESLVADNCVTLVWRMPDEDSKIDHYVLEYRKTNFEGPPRVKEDQPWMVIEGIKQTEYTLTGLRFDMKYMNFRVKACNKAVAGEFSEPVTLETRAFMFRLDASTCHQNLKVEELSVEWDAMGGKVQDIKAREKDGKGRTASPVNSPARCLQSPKRMPSARGGRDRFTAESYTVLGDSLIDGGDHYWEVRYDRDSKAFGVGVAYRTLGKFDQLGKTSSSWCIHLNNWLQVSFTAKHNNKAKVLDMPVPDCIGVYCNFHEGFLSFYNARTKQLLHSFKAKFTQPVLPAFMVWCGSFHVYSGLQVPSAVKCLQKRNSATSSSNASLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSP4</accession>
    <name>A0A452HSP4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HotDog ACOT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HSP4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018070"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020606.1">
        <property type="protein sequence ID" value="ENSGAGP00000018070.1"/>
        <property type="gene ID" value="ENSGAGG00000013391.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000012">
        <property type="entry name" value="Acyl-coenzyme A thioesterase 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000016">
        <property type="entry name" value="Acyl-coenzyme A thioesterase 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655:SF0">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="8">
        <location>
            <begin position="90"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="8">
        <location>
            <begin position="295"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="433" mass="49214" checksum="DBFFC860421DD848" modified="2019-05-08" version="1">MLALRCTVGKAPSLLARALTSMTNGFQSPPDMSEGHLPIHVNNVRSRLRAIVGASTNWSDHVQAMHERKDLHSLLAKQQEDLPPRKMNDSYIEVILPLGSQPELREKYLNVHNTVRFGRILEDLDSLGVLICYTHTKHEASQRSPLSIVTALVDKIDLCKKVIYPDCDIKFTGNVSWVGKTSMEVKMHMLQVGLHDDTYSSVLDATFVMVARDPENKRSAFVNPLIPEGQEEEEIFRKGELNKMRRVDFSTASLLKMAPTAEERNIVHNIFLNTLDTRTVSFRSRVLPPNSVWMEDAKLKSLVICHPQERNIFNRIFGGFLMRKAFELGWATASSYGGARPYVVAVDDIMFQRPVEIGSLLLLSAQVCYTAENYIQIRVHSEVYDSDTREHKTTNVFHFTFMSEREVPQIVPKTYGESMLYLDGKRHFSATTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTU1</accession>
    <name>A0A452HTU1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Plexin-A2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Coreceptor for SEMA3A and SEMA6A. Necessary for signaling by SEMA6A and class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000018525"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021115.1">
        <property type="protein sequence ID" value="ENSGAGP00000018525.1"/>
        <property type="gene ID" value="ENSGAGG00000012624.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01180">
        <property type="entry name" value="IPT_plexin_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01179">
        <property type="entry name" value="IPT_plexin_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12790">
        <property type="entry name" value="RasGAP_plexin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11272">
        <property type="entry name" value="Sema_plexin_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000005">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000006">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000123">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000006">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000071">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000131">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000339">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000018">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000032">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042826">
        <property type="entry name" value="Plexin-A2_sema"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013548">
        <property type="entry name" value="Plexin_cytoplasmic_RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046800">
        <property type="entry name" value="Plexin_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041019">
        <property type="entry name" value="TIG1_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041362">
        <property type="entry name" value="TIG2_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF37">
        <property type="entry name" value="PLEXIN-A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08337">
        <property type="entry name" value="Plexin_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20170">
        <property type="entry name" value="Plexin_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18020">
        <property type="entry name" value="TIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17960">
        <property type="entry name" value="TIG_plexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1231"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="18">
        <location>
            <begin position="22"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="1257"/>
            <end position="1284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1887" mass="210218" checksum="440110EAE687B041" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HU72</accession>
    <name>A0A452HU72_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VWFC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HU72"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018655"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021268.1">
        <property type="protein sequence ID" value="ENSGAGP00000018655.1"/>
        <property type="gene ID" value="ENSGAGG00000013807.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042979">
        <property type="entry name" value="VWC2/VWC2L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059152">
        <property type="entry name" value="VWC2L_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46252:SF4">
        <property type="entry name" value="BRORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46252">
        <property type="entry name" value="BRORIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23333">
        <property type="entry name" value="VWC2L_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23334">
        <property type="entry name" value="VWC2L_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019554773" description="VWFC domain-containing protein" evidence="7">
        <location>
            <begin position="30"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="8">
        <location>
            <begin position="225"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="34"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="90"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="286" mass="31516" checksum="A4B311D1E289E201" modified="2019-05-08" version="1">MPSSTAMAIGALSSSLLVTCCLMVALCSSTIPVQKLAKAPDKQEIKTSQEKRDHTSTRPDSQSQTGPGKMNEIGRNGREEGTRDWKSKGNRNYSNKESWTRQKQIWNSQGTSSKSGSIQAQEQRDGIPEEPQAAEESSLPEVVASTTPEPPEEYAYPDYRGKGCVDESGFVYAIGEKFTPGPSACPCLCTDEGPLCIQPECPRLHPRCIHVDTSQCCPQCKERKNYCEFRGKTYQTLEEFMVSPCEKCRCEANGEVLCTVSACPQTECVDPVYEPDQCCPICKNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUV4</accession>
    <name>A0A452HUV4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HUV4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018889"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021535.1">
        <property type="protein sequence ID" value="ENSGAGP00000018889.1"/>
        <property type="gene ID" value="ENSGAGG00000013983.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019127600" description="C-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="32"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="41"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="125" mass="14495" checksum="491BAAA93430566A" modified="2019-05-08" version="1">LLSWDLWSCFVLNALPVFFLFSPAMPEPVQSYSCPLFWTEYEGYCYRYFPINKTWAEADLYCAEFSIGIKSAKLASIHSWEENVFVYDLVNSRVPGIPTDIWMGLHDLRQVRNPNSSSQPTKLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWJ2</accession>
    <name>A0A452HWJ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfate transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Solute carrier family 26 member 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>bromide(in) + chloride(out) = bromide(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:75335"/>
            <dbReference type="ChEBI" id="CHEBI:15858"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>iodide(in) + chloride(out) = iodide(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72379"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>nitrate(in) + chloride(out) = nitrate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:75339"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>oxalate(in) + sulfate(out) = oxalate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:72275"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>oxalate(out) + 2 chloride(in) = oxalate(in) + 2 chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:75095"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>sulfate(out) + 2 chloride(in) = sulfate(in) + 2 chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:75091"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HWJ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019484"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022187.1">
        <property type="protein sequence ID" value="ENSGAGP00000019484.1"/>
        <property type="gene ID" value="ENSGAGG00000014365.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000015">
        <property type="entry name" value="Sulfate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="443"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="494"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="19">
        <location>
            <begin position="550"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="707" mass="78085" checksum="6D5B25D5BDD72596" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HX39</accession>
    <name>A0A452HX39_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatase 2A regulatory subunit B56 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HX39"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019744"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022494.1">
        <property type="protein sequence ID" value="ENSGAGP00000019744.1"/>
        <property type="gene ID" value="ENSGAGG00000014563.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000010">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A 56 KDA REGULATORY SUBUNIT BETA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="497" mass="57795" checksum="7404AD9A4D8BC96A" modified="2019-05-08" version="1">METKLPPAGTPTSPSSPGMSPVPPPDKVDGFSRRSLRRSRQRRSHSSSQFRYQSNQQELTPLPLLKDVAVAELHELLCKKLQQCCVLFDFLDCVADLKGKEIKRAALNELVECVATNRGVLIEPVYPEIIKMISVNIFRTLPPTENPEFDPEEDEPNLEPSWPHLQLVYEFFLRFLESPDFQPSVAKRYVDQKFVLMLLELFDSEDPREREYLKTILHRVYGKFLGLRAYIRKQCNNIFLRFIYETEHFNGVAELLEILGSIINGFALPLKTEHKHFLVRVLIPLHSVKSLSIFHAQLAYCVVQFLEKDATLTEHVIRGLLKYWPKTCTQKEVMFLGEIEEILDVIEPSQFVKVQEPLFKQIARCISSPHFQVAERALYFWNNEYILSLIEDNCHTVLPAIFGTLYRVSKEHWNQTIVSLVYNVLKTFMEMNGKLFDELTASYKVEKQQELKKEKERQELWKQLDELQLKKLQGLEEAQMNRINLQHSLGLQSGNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HX80</accession>
    <name>A0A452HX80_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HX80"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019748"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022498.1">
        <property type="protein sequence ID" value="ENSGAGP00000019748.1"/>
        <property type="gene ID" value="ENSGAGG00000014420.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000039">
        <property type="entry name" value="Brain-specific serine protease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF170">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="100"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="382" mass="41136" checksum="3F7F98F3EAAFF55C" modified="2019-05-08" version="1">MPTLGASPPAVGVPGVRFSTGTPGQKGTLKSLVGGPAGLRQASPYLSWHRTTPWKWPAGPAPRLGGHGAPCAAPAPSTSSTLPLAGNRICGQPRLPLNRLVKGQDTVPGEFPWQASIQWRGVHMCGGSLINDEWVVTAAHCFFQVQNLTQYRVVLGAHQLLKPGPSACACPVQRIITNPRYAGQTTSGDIALVQLGRRLNFTDYILPICLPDASVHFPPGKVCWVTGWGHVRSWVDLPSPQTLQKLKVPIIDSKTCRILYLTNMQNRLPHRNIQDDMICAGYAEGMRDACNGDSGGPMACIVGDVWVLAGIVSWGEGCAIKNRPGVYSRLTSYQSWIQEPGCFSALAAHLRATSSGSRAFCCLLCPTCILDMLRPGFFFLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HY89</accession>
    <name>A0A452HY89_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HY89"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020129"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022943.1">
        <property type="protein sequence ID" value="ENSGAGP00000020129.1"/>
        <property type="gene ID" value="ENSGAGG00000014861.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000233">
        <property type="entry name" value="Protocadherin gamma-A3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF133">
        <property type="entry name" value="PROTOCADHERIN ALPHA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019194682" description="Cadherin domain-containing protein" evidence="15">
        <location>
            <begin position="30"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="694"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="34"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="134"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="243"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="351"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="456"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="580"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="796"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="831" mass="90195" checksum="0BB0272FEE00D010" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYM1</accession>
    <name>A0A452HYM1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYM1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020280"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023105.1">
        <property type="protein sequence ID" value="ENSGAGP00000020280.1"/>
        <property type="gene ID" value="ENSGAGG00000014956.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15221">
        <property type="entry name" value="7tmA_OR52B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000006">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF32">
        <property type="entry name" value="OLFACTORY RECEPTOR 52B6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="48"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="332" mass="37560" checksum="239A45C393015BA5" modified="2019-05-08" version="1">LAVLNMTTLNQSSFDPITFILTGIPGMEESHIWISVPVCLMYVVALFANSVLLFIIITERSLHEPMYLFLAMLAVADLLLSTTTVPKMLAIFWFSAKEISFDACVTQMFFTHFSFIAESTVLLAMAFDRYVAICYPLRYPTILTHSVIGKIAVTAVLRGLCIMFPPIFLLKRLPYCGHTVMPHTYCEHMGIARLACADITANIWYGFTTTLLSSGLDVMFIAVSYGLILRAVFQLPSKDARLKALRTCGSHLCVILMFYTPAFFSFLTHRFGHSIPGHIHILLANLYVIVPPMLNPIVYGVRTRQIRERILKRVINVFYRYPAVTNHSLLWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1E3</accession>
    <name>A0A452I1E3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I1E3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021317"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024279.1">
        <property type="protein sequence ID" value="ENSGAGP00000021317.1"/>
        <property type="gene ID" value="ENSGAGG00000015652.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000024286.1">
        <property type="protein sequence ID" value="ENSGAGP00000021322.1"/>
        <property type="gene ID" value="ENSGAGG00000015652.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000383">
        <property type="entry name" value="armadillo repeat-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46241">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 4 ARMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46241:SF1">
        <property type="entry name" value="OUTER DYNEIN ARM-DOCKING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="548"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="892"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="387"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1055" mass="117063" checksum="C3CF57361681F508" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1G7</accession>
    <name>A0A452I1G7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">cis-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452I1G7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021306"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024266.1">
        <property type="protein sequence ID" value="ENSGAGP00000021306.1"/>
        <property type="gene ID" value="ENSGAGG00000015642.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01863">
        <property type="entry name" value="Rab18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000430">
        <property type="entry name" value="Probable Ras-related protein Rab-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="206" mass="23135" checksum="2E9E62852E99C946" modified="2019-05-08" version="1">MDEDILTTLKILIIGESGVGKSSLLLRFTDDTFDPELAATIGVDFKVKTISVDGNKAKLAIWDTAGQERFRTLTPSYYRGAQGVILVYDVTRRDTFVKLDNWLNELETYCTRNDIVKMLVGNKIDKENRAVDRNEGLKFARKHSMLFIEASAKTCDGVQCAFEELVEKIIQTPGLWESESQNRGVKLSNQEEGYGERACGGYCSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I249</accession>
    <name>A0A452I249_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibroblast growth factor-binding protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I249"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024548.1">
        <property type="protein sequence ID" value="ENSGAGP00000021555.1"/>
        <property type="gene ID" value="ENSGAGG00000015817.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010510">
        <property type="entry name" value="FGF1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15258">
        <property type="entry name" value="FGF BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15258:SF1">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06473">
        <property type="entry name" value="FGF-BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019353518" evidence="8">
        <location>
            <begin position="19"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="117"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="123"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="221" mass="24638" checksum="DE45226AACD0AE13" modified="2019-05-08" version="1">MKTVILLLMMICCMGGLGQKQTQKKRSNGEEILFQTKVKDACTMNMSGNGEMKLRIECKNQGKSYWCEYTGKPSVCRPFNNNPKVYWNQIALELRKLPNACQSTLVLKPSMCQKAPTDAQMKQVASSMKPNQNPSQQADTANQGKSVQKSSASLKQVKETQAGKSSAKKAGRPKPSTLPLVKPTQHGQESENETEVMKLAREHCWESLHTFCSYIISIFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I436</accession>
    <name>A0A452I436_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the Nogo receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I436"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022280"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025392.1">
        <property type="protein sequence ID" value="ENSGAGP00000022280.1"/>
        <property type="gene ID" value="ENSGAGG00000016366.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000018">
        <property type="entry name" value="Reticulon 4 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF196">
        <property type="entry name" value="RETICULON 4 RECEPTOR LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019203379" description="LRRCT domain-containing protein" evidence="17">
        <location>
            <begin position="30"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="18">
        <location>
            <begin position="263"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="288"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="431" mass="47584" checksum="D5F36D547BF1D807" modified="2019-05-08" version="1">MLPRPVRLLHGSITPLFCLLVLLVTLPLGAPTCPMLCTCYFSPPTVSCQANNFSSVPQVLPPNAQRLFLQNNLIGALQAGTFGPSTITLWLYSNNISSIQPGTFHHLPALEELDLGDNRHLRSLDPDTFWGLERLQSLHLYRCQLSSLPSTLFRSLFSLQYLYLQENNLLYLQDDLFVDLANLSHLFLHGNKIRVLSEHVFRGLGGLDRLLLHVNRLHSVHRWAFRDLAKLTILYLFNNSLATLPGETLAELPSLEFLRLNNNPWACDCRARSLWAWFQHARVSSSDVACTSPPERQGRDLRHLSPADFRDCPPARPAGGTKPKWGGEAEAGQPHSNSSSNHLYGLGEAGAPPADPSSFYRDLPASDIRSPKYDSPTEVDYWAGYGSEEGRLREGCTGPGCSPLDSGACWPGTLPAALPPLLLLLLGLPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7M0</accession>
    <name>A0A452I7M0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">T-complex protein 1 subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">CCT-epsilon</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of the chaperonin-containing T-complex (TRiC), a hexadecamer composed of two identical back-to-back stacked rings enclosing a protein folding chamber. Each ring is made up of eight different subunits: TCP1/CCT1, CCT2, CCT3, CCT4, CCT5, CCT6A/CCT6, CCT7, CCT8. Interacts with PACRG. Interacts with DNAAF4. Interacts with DLEC1. Interacts with SPMAP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I7M0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023626"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026919.1">
        <property type="protein sequence ID" value="ENSGAGP00000023626.1"/>
        <property type="gene ID" value="ENSGAGG00000017319.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03339">
        <property type="entry name" value="TCP1_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000053">
        <property type="entry name" value="T-complex protein 1 subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000028">
        <property type="entry name" value="T-complex protein 1 subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000003">
        <property type="entry name" value="T-complex protein 1 subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000049">
        <property type="entry name" value="T-complex protein 1 subunitTheta, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012718">
        <property type="entry name" value="Chap_CCT_epsi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02343">
        <property type="entry name" value="chap_CCT_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 18" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="541" mass="59729" checksum="5F06223EF2BFF04A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7Q0</accession>
    <name>A0A452I7Q0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peripherin 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I7Q0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023557"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026844.1">
        <property type="protein sequence ID" value="ENSGAGP00000023557.1"/>
        <property type="gene ID" value="ENSGAGG00000017269.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000830">
        <property type="entry name" value="Peripherin/rom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00218">
        <property type="entry name" value="PERIPHERNRDS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="28015" checksum="7430EA667E2B51C9" modified="2019-05-08" version="1">MAVFKVKFTKTKRHKLAQILWILNWISVATGVILFSLGLFLKIEIKKRSEVMAKGEINSVPNMLISVGVIACIINFLGGKICYDCSDTTKFSRWRLVMLPYIVCTFCFTFCILVGALTCYTMRNELEESLYLGLRDAIKFYKDTDIPGRCFLKKTIDLLQIGFQCCGNNGFRDWFEIQWISVRYLNMASKEVLDYNVGKLKATTKFEAQCASILEINLLLEHCNLLTPPLYNSLGREMKNYLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I921</accession>
    <name>A0A452I921_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-mRNA polyadenylation factor Fip1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FIP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I921"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024165"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027514.1">
        <property type="protein sequence ID" value="ENSGAGP00000024165.1"/>
        <property type="gene ID" value="ENSGAGG00000017677.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007854">
        <property type="entry name" value="Fip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051187">
        <property type="entry name" value="Pre-mRNA_3'-end_processing_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035352">
        <property type="entry name" value="TSX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484">
        <property type="entry name" value="FIP1-LIKE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484:SF8">
        <property type="entry name" value="PRE-MRNA 3'-END-PROCESSING FACTOR FIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05182">
        <property type="entry name" value="Fip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17397">
        <property type="entry name" value="TSX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA polyadenylation factor Fip1" evidence="6">
        <location>
            <begin position="148"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="192"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="239"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="304"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="365" mass="39420" checksum="9854198B6DE6C6EE" modified="2019-05-08" version="1">MATELEPPASGAAPLEAEEDEEHWLYGDDTTGKQEDGPIARHAESSHPLQDAPQESRPVTSEDQEMSQHALPSGEDEEEDSDSDSDDDDDVKVTIGNIKTGAPSYMGTPMNLNLKTGRGYGTSSSAKLQPKGIDLDATGNINGLPVIEVDLDSFEDKPWRKPGADLSDYFNYGFNEDTWKAYCEKQRRLQLGLDPSPPISSENKITVQQGRTGNAEKEPENNIIKTEFKTDFVALIGGRMKAGPPPNRKLGGTIDVIGGQAGTIRRVEGRRRDKHASEENPIQVLGDHGNKPQPPQQPQQPSQPQQPPQQQPFVPPAGPPPPPIAGPPPPHFLHPPPPVTSVPPPLHPPGLPPPGPIPGRLLICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9J4</accession>
    <name>A0A452I9J4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I9J4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024213"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027565.1">
        <property type="protein sequence ID" value="ENSGAGP00000024213.1"/>
        <property type="gene ID" value="ENSGAGG00000017703.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051435">
        <property type="entry name" value="RING_finger_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791:SF17">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="28"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="104"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="189" mass="20717" checksum="044FBA37CAA29AE1" modified="2019-05-08" version="1">MRREKLRSPGGEAAPAEGRPLTQQRLECKICYNYFDLERRAPKLLECLHTFCQECLSQLHLRAAHGTICCPVCRHRTALPDQRVHSLPVNTKLAEAFPLQLRARDPLPQDRLPPVPRPAAGPQSRPAPHEAGPRWGRGCLLLLQPPPEAAPPTPRESGGPAGYSARAFPSTRLPGLVPSGLGGQREGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9N6</accession>
    <name>A0A452I9N6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I9N6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024399"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027770.1">
        <property type="protein sequence ID" value="ENSGAGP00000024399.1"/>
        <property type="gene ID" value="ENSGAGG00000017828.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000001">
        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF18">
        <property type="entry name" value="COMPLEMENT C1Q SUBCOMPONENT SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0176">Collagen</keyword>
    <keyword evidence="5" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="C1q" evidence="7">
        <location>
            <begin position="153"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="75"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="288" mass="30503" checksum="B165D378C6C2BF8F" modified="2019-05-08" version="1">MWPVPLLAQPFSAHANFCFPSAGSLPSWTETCPASLLQDTASAGADMMLWAVLFCLAAVPLASADRCQGHAAIPGIPGVPGQPGSNGKDGVDGPKGEKGPPGRVDDEMELGEKGEPGSPGHPGKVGPKGPVGSKGSPGPTGPPGPMGESGDFKTTLRSAFSAARTISMLPRRDQPIRFDRIITNENGHYENRYGRFTCKIPGLYYFTYHLTSRGNLCINIKKGLGTKGEKVVTFCDYVHNVYQVTTGGVVLRLQAEESVWLEPTEKNSLVGIEGADSIFSGFLLFPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9Q6</accession>
    <name>A0A452I9Q6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I9Q6"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027752.1">
        <property type="protein sequence ID" value="ENSGAGP00000024383.1"/>
        <property type="gene ID" value="ENSGAGG00000017816.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="197" mass="19736" checksum="D9E52846E750872B" modified="2019-05-08" version="1">DSPSSVRNATATTTVAQQTASSQHPSATATPPQSPLSATQHPSTTATPPQSPVATSRDTSLSNRTTAAAPASSRTPESASSLPLSSASPSHTNTSATPAASSSETAGEVSKPSSPSPPAVSSLKQPTLSLSPGLVAVICIFVAILVIAAGVVVVKLCRRREPAFEKLDEVPMGGSLAQRLGGTFQAQCLGIVLISSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAT1</accession>
    <name>A0A452IAT1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RAB17, member RAS oncogene family</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IAT1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024781"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028199.1">
        <property type="protein sequence ID" value="ENSGAGP00000024781.1"/>
        <property type="gene ID" value="ENSGAGG00000018081.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="134"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="21167" checksum="654C67022CAD29DC" modified="2019-05-08" version="1">AAQAVGAGRPSTEQAYISKVVLLGRTAMGKSSTAFRYVKNDIRESMTTVGCSFFTQLGTAGQEMYHSVCHLYYRGANAAFLVYDIARKATFVRAKLWLRELEKEFLPDELVIVLVSNKADLAAQREVTFEVKSASPSRSSLPSPPSSLPLLSRPAEREAQPGSQEMPGVRLSPRSHWPSRGRLRCCCGGWVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB61</accession>
    <name>A0A452IB61_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IB61"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024929"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028376.1">
        <property type="protein sequence ID" value="ENSGAGP00000024929.1"/>
        <property type="gene ID" value="ENSGAGG00000018226.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019182144" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="129" mass="14064" checksum="85AEC26167DE5D3A" modified="2019-05-08" version="1">MLWAPLIVLLGTWCTASSAQPVLTQPPSIAASPGQTVKISCSMSSGVTVQSYPQTWQQQTPGSPPRHLLYYYSSMSRGSGVPDRFSGSKESSSNVWYLTITNVQAEDEADYYCAVWYGSGGFVKQPQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBM2</accession>
    <name>A0A452IBM2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IBM2"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028370.1">
        <property type="protein sequence ID" value="ENSGAGP00000024923.1"/>
        <property type="gene ID" value="ENSGAGG00000018218.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="20265" checksum="EE80A08FD06CCEA7" modified="2025-10-08" version="2">MPVVTQEPSKSVTPGGTVTLSCSLSTGAISTSNYPSWFQQKPGSAPPLLIYSTNTRPSGIPAWFSGSISGTRLCSSAAYIQHQYQTSGIPARFSGWGTETKTTPVFPTSSPPAWALHWLHNLLPPKVRPRESRDCLGSLAPSPGPALRDTHTGAAGHKEAEDTVQDHLGSKTSTPNPAGTNTAHETWEFPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IEB0</accession>
    <name>A0A452IEB0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alpha-macroglobulin receptor-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IEB0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026045"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029621.1">
        <property type="protein sequence ID" value="ENSGAGP00000026045.1"/>
        <property type="gene ID" value="ENSGAGG00000019023.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF165">
        <property type="entry name" value="ALPHA-2-MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="1">
        <location>
            <begin position="274"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="370" mass="41069" checksum="26B066730FEB1988" modified="2019-05-08" version="1">VRQNKWMWPNMASYVSLLTQHSVVRNALFCLETTAEQGGNHVYTRALLAYAFALAGKEEKHRALLDSLDKEAVKEDGSIHWQRPGKEPKAELPFYQPRAPSAEVEMTSYVLLAYLTMQPAPSQEDLSLATQIAKWISKQQNPNGGFSSTQDTVVALQALSQYGVSAYAKSGGASTVTLQSTGNFQFQFQVDHTNRLLLQHMALPEVPGDYSMGVTGEGCVYVQTNLRYNVLPKLGETPFAMEVHTVPETCDSAKAQRTFNIAINISYTGKRPVSNMVIVDVKMLSGFIPVKSSVRKLEGHQHIHHTELSTNHVLLYMEQVSNVTQSFSFAVEQDFPVQSLKPALAKVYDYYETDEFAIAEYSAPCSKGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGN5</accession>
    <name>A0A452IGN5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL9m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L9, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IGN5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027037"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030719.1">
        <property type="protein sequence ID" value="ENSGAGP00000027037.1"/>
        <property type="gene ID" value="ENSGAGG00000019667.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.5.10:FF:000005">
        <property type="entry name" value="39S ribosomal protein L9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="entry name" value="Ribosomal protein L9, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056864">
        <property type="entry name" value="MRP-L9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_bL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_bL9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_bL9_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054302">
        <property type="entry name" value="Ribosomal_bL9m_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25131">
        <property type="entry name" value="bL9m_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22078">
        <property type="entry name" value="Ribosomal_bL9m_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL9m N-terminal" evidence="11">
        <location>
            <begin position="5"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal protein L9" evidence="9">
        <location>
            <begin position="47"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein bL9m C-terminal" evidence="10">
        <location>
            <begin position="105"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="267" mass="29958" checksum="332EE619D4F3506B" modified="2019-05-08" version="1">MQGTVVVERWWKVPLSKEGRPPRLHPRRHRIYRLVEDTKNQPKGELEVILTQSVDNLGSRGDIVSVKKSLARNKLLLEGLAVYASPENKKMFEEEMRLREEGKLERLQTQSGEKAIQFLRSCRLEVGMKNNVKWELNNEIVARHFLKNLNVFVKPHAVKLPDEPITRWGEYWCEVTVSARFFGKGADHLATNSSNFLVPTLPVTLHCVRASERGIRTQILISALATGSLVSLGKSLPCSVPQFPPSVTQGKYLSASLGGGRGGFVAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IH09</accession>
    <name>A0A452IH09_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IH09"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027163"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030858.1">
        <property type="protein sequence ID" value="ENSGAGP00000027163.1"/>
        <property type="gene ID" value="ENSGAGG00000019752.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020440">
        <property type="entry name" value="IL-17_chr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010345">
        <property type="entry name" value="IL-17_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06083">
        <property type="entry name" value="IL17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01932">
        <property type="entry name" value="INTRLEUKIN17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019255286" evidence="7">
        <location>
            <begin position="21"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="206" mass="23222" checksum="F2B45F8239D6E33D" modified="2019-05-08" version="1">MDWAPSLLLFCALSVSFTWAATTEPKDHSKEAKGKRKGAGRDEVQLPEAQTPGPDPVLKRNAMISAADSSYTQLEDYEQSIQEMVSQLRNSSEPADSKCQVNLRLWRSNKRSLSPWTYSINHDAMRIPADIPEAQCLCTGCINPFTMQEDRTIVSIPIYSKLPVRRLLCHPPEGSGTKTRKKKKKRCHKEYKMVMETIAVGCTCIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHU0</accession>
    <name>A0A452IHU0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027212"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030916.1">
        <property type="protein sequence ID" value="ENSGAGP00000027212.1"/>
        <property type="gene ID" value="ENSGAGG00000019770.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14068">
        <property type="entry name" value="STKc_LRRK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001216">
        <property type="entry name" value="Leucine-rich repeat kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001161">
        <property type="entry name" value="Leucine-rich repeat serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000215">
        <property type="entry name" value="leucine-rich repeat serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000219">
        <property type="entry name" value="Leucine-rich repeat serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000313">
        <property type="entry name" value="Leucine-rich repeat serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1390:FF:000001">
        <property type="entry name" value="Leucine-rich repeat serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000656">
        <property type="entry name" value="Leucine-rich repeat serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000110">
        <property type="entry name" value="Leucine-rich repeat serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000179">
        <property type="entry name" value="leucine-rich repeat serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1390">
        <property type="entry name" value="ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056593">
        <property type="entry name" value="ANK_LRRK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056597">
        <property type="entry name" value="ARM_LRRK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056602">
        <property type="entry name" value="Beta-prop_LRRK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032171">
        <property type="entry name" value="COR-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057263">
        <property type="entry name" value="COR-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020859">
        <property type="entry name" value="ROC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051420">
        <property type="entry name" value="Ser_Thr_Kinases_DiverseReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005">
        <property type="entry name" value="LEUCINE RICH REPEAT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DDB_G0278509-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23745">
        <property type="entry name" value="ANK_LRRK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23744">
        <property type="entry name" value="ARM_LRRK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23748">
        <property type="entry name" value="Beta-prop_LRRK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16095">
        <property type="entry name" value="COR-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25497">
        <property type="entry name" value="COR-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08477">
        <property type="entry name" value="Roc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51424">
        <property type="entry name" value="ROC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Roc" evidence="16">
        <location>
            <begin position="1284"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="1835"/>
            <end position="2106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1862"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2182" mass="246497" checksum="0B9338425F87A057" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJ94</accession>
    <name>A0A452IJ94_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJ94"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027937"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031730.1">
        <property type="protein sequence ID" value="ENSGAGP00000027937.1"/>
        <property type="gene ID" value="ENSGAGG00000020286.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08340">
        <property type="entry name" value="DED_c-FLIP_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000016">
        <property type="entry name" value="CASP8 and FADD-like apoptosis regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001875">
        <property type="entry name" value="DED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48169:SF3">
        <property type="entry name" value="CASP8 AND FADD LIKE APOPTOSIS REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48169">
        <property type="entry name" value="DED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01335">
        <property type="entry name" value="DED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00031">
        <property type="entry name" value="DED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50168">
        <property type="entry name" value="DED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DED" evidence="4">
        <location>
            <begin position="6"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="DED" evidence="4">
        <location>
            <begin position="96"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="5">
        <location>
            <begin position="248"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="462" mass="53217" checksum="92AC8B607D0C49B7" modified="2019-05-08" version="1">MTKCQVPAALIHQIEQELDEDEKEMMFFLCRDLIPDLSNTDIRELLGMLNEKGKLLLGLSELLYRVRRFDLLRRALRTERTAVETDLARYPRMVSDYRVLMVEINEELDKKEVGSLTFLLKDYVPRMKMAKDKSFLAVVSDLEKLNLVAPNQLDLIEDCLQNIHRIDLKKKIQRYKCAALSNSGSQPMYINALQASLPNLTLIDPPYSSGVSMQQITEPVHTSVQESGATSSQGNKKVYVHFRLFYILEDTFKALGFEVHCYQYLSVEAMNQTFHEVARLQKHRDYDSFICILVSRGSPQSIFCTDQTFPGYPLDRVKKFFTGDSCPELLGKPKLFFIQSYIVPADQQEPTSLLEVDGNEQKITANSRRARNCSIPPVADIFWSQCKVDVSVLERSPSSSSHYLRFLAELLCNPHKRKLPLLDIHIELNSRVYDCNRTSDPQQQYSLLLQHTLRKKLFLFPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJ95</accession>
    <name>A0A452IJ95_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IJ95"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027905"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031694.1">
        <property type="protein sequence ID" value="ENSGAGP00000027905.1"/>
        <property type="gene ID" value="ENSGAGG00000020266.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031630">
        <property type="entry name" value="CCDC117"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36128">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36128:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15810">
        <property type="entry name" value="CCDC117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="341" mass="37456" checksum="0D6B096EBDC43431" modified="2019-05-08" version="1">MKMATLGRSFQEVPPVEFPQAPATLPDSTFQPGIIQTESQPGAVGHEYPQQTIVVTAGNRNNIVEGSDLSSLYLQSNLDLASLAATGSYNEGLTVATVSFTNSEKPPVLLQQQNCTQVSLRCGKKHKLEEEVDCCPVKKKRLIETVHYSPNPSTEEILCAGQQAAGEVARQCIDSLHEAGLLEIPCEDMDQTIGEHQCEVARRKLQEIEDRIIDEDEDVHADGNTSNLPTLVLSDTLQKGLRRDFDQVLTKKIIESMSRPSMELVLWKPLPEFLTDKLKSVSVAKNYKQQGTEGCQAKQSTLGAAFQPQTETFSEAQQTVMSPGLYSSLGTSVCVEEEMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJY5</accession>
    <name>A0A452IJY5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJY5"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032102.1">
        <property type="protein sequence ID" value="ENSGAGP00000028247.1"/>
        <property type="gene ID" value="ENSGAGG00000020528.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045028">
        <property type="term" value="F:G protein-coupled purinergic nucleotide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15151">
        <property type="entry name" value="7tmA_P2Y13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000049">
        <property type="entry name" value="G-protein coupled receptor 87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008109">
        <property type="entry name" value="P2Y13_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233:SF10">
        <property type="entry name" value="P2Y PURINOCEPTOR 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233">
        <property type="entry name" value="P2Y PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01735">
        <property type="entry name" value="P2Y13PRNCPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="65"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="358" mass="40631" checksum="75EBB0842C41C980" modified="2019-05-08" version="1">MHPKMITSQTSTTTTPTVDLPAPNCNIITTNGTSSFSGQCHRDTRIAHVAFPILYIVIFLTGVILNSLAIRAFFQIQSTSTFIVYLKNTLVSDFIMTLMLPLKILTDSGLGPWQLKAFVCRFSAVIFYETMYVSIVLLGLIAFDRFLKIVRPFGKFWVQNVTAAQILSGLVWLFFFVLSLPNMILSNNKATPLSVKKCASLKNPLGLKWHAAVNYICQFIFWTVLILMLLFYAIITKKVYNSYIKTQKKDSKSKQRINGKVFIIVAVFFICFAPFHFCRVPYTLSQTGNTSDCNVQNLLFIAKESTLWIATTNICMDPLIYIILCRSFVEKAFCVKMKKIRSTMRENTTVPLDTRISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKK5</accession>
    <name>A0A452IKK5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tetratricopeptide repeat protein 36</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTC36 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IKK5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028359"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032217.1">
        <property type="protein sequence ID" value="ENSGAGP00000028359.1"/>
        <property type="gene ID" value="ENSGAGG00000020582.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006570">
        <property type="term" value="P:tyrosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000213">
        <property type="entry name" value="Tetratricopeptide repeat domain 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038906">
        <property type="entry name" value="TTC36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21405">
        <property type="entry name" value="CDNA SEQUENCE BC021608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21405:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="190" mass="20629" checksum="4B25A5A575B4E1CE" modified="2019-05-08" version="1">MATAKDRAILQTIFNPSTPFGDIPGLDEEEEAVAIEGEDGAFASELLEQVKDLELQGVLAAESGDVNTALERFSQAIQLLPERASCYNNRAQALRLRGDVAGALQDLDTALHLSGGTGRVACQCFVQRGLINVLEGHEDNARQDFEQGAKLGSAFARHQLVLMNPYSALCNQMLLEMMRKLQNPDIQGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IL39</accession>
    <name>A0A452IL39_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IL39"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028736"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032647.1">
        <property type="protein sequence ID" value="ENSGAGP00000028736.1"/>
        <property type="gene ID" value="ENSGAGG00000020831.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20037">
        <property type="entry name" value="FH_FOXS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000016">
        <property type="entry name" value="Forkhead box protein I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047364">
        <property type="entry name" value="FH_FOXS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF370">
        <property type="entry name" value="FORKHEAD BOX PROTEIN S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="7">
        <location>
            <begin position="18"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="18"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="311" mass="35199" checksum="8F81307C97EDEA8C" modified="2019-05-08" version="1">PLFPPPPGAPSQMGEATKPPYSYIALITMAIQSTPEKRITLSGIYRYIMGRFTFYRDNKQGWQNSIRHNLSLNECFVKVPRDDKKPGKGNYWTLDPDCYNMFENGSFLRRRRRFTRKRGLRDPGQVSLLTPRKYLQKPRQACLYPLEGPEPKPQLFEKQPCYPGLEGHLPETQGQALPRLKETVFGGLQPPIQIAQPPYTAPGERVTQQHPQLTRDIKEPLSGNYQCRVQALSFCMNERACNSALEHLLTVSPGAAPVTPIQPPPFRPAVQLQGEQKESWAGSPFSLPGGNGYQLGLPHCLYRTPGMFFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IL41</accession>
    <name>A0A452IL41_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Interacts with ALKBH4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IL41"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028588"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032467.1">
        <property type="protein sequence ID" value="ENSGAGP00000028588.1"/>
        <property type="gene ID" value="ENSGAGG00000020709.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03754">
        <property type="entry name" value="proteasome_alpha_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000020">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034642">
        <property type="entry name" value="Proteasome_subunit_alpha6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4 9 10" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="11">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="246" mass="27455" checksum="B53B20D43FEC957C" modified="2019-05-08" version="1">MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVIVTQKKVPDKLLDSSTVTHLFRITETIGCVMTGMTADSRSQVQRARYEAANWKYKYGYEIPVDMLCKRIADISQVYTQNAEMRPLGCCMILIGIDEEHGPQVYKCDPAGYYCGFKATAAGVKQTESTSFLEKKVKKKYDWTFEQTVETGITCLSTVLSIDFKPSELEVGVVTVENPKFRILTEAEIDTHLVALAERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILC8</accession>
    <name>A0A452ILC8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Myosin is a hexamer of 2 heavy chains and 4 light chains.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILC8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028715"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032624.1">
        <property type="protein sequence ID" value="ENSGAGP00000028715.1"/>
        <property type="gene ID" value="ENSGAGG00000020814.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000010">
        <property type="entry name" value="Myosin regulatory light chain 2, atrial isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000007">
        <property type="entry name" value="Putative myosin regulatory light chain sqh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050403">
        <property type="entry name" value="Myosin_RLC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23049">
        <property type="entry name" value="MYOSIN REGULATORY LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="94"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="166" mass="18739" checksum="F4D171C4B439548F" modified="2019-05-08" version="1">MSPKKAKKKTEGGGSNVFSMFDQTQIQEFKEAFTIMDQNRDGFIDKADLRDTFAALGRLNVKNEELEAMVQEAPGAINFTIFLSMFGEKLKGTDPEETILNAFKIFDPEGKGHIKADYIKEMLMTQADRFSQEEVSQMFAAFPPDVSGNLDYKNLCYVITHGEEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452INJ9</accession>
    <name>A0A452INJ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">A-type voltage-gated potassium channel KCND2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Potassium voltage-gated channel subfamily D member 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Voltage-gated potassium channel subunit Kv4.2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452INJ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029475"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033470.1">
        <property type="protein sequence ID" value="ENSGAGP00000029475.1"/>
        <property type="gene ID" value="ENSGAGG00000021293.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005250">
        <property type="term" value="F:A-type (transient outward) potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905030">
        <property type="term" value="F:voltage-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18418">
        <property type="entry name" value="BTB_POZ_KCND2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000152">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003975">
        <property type="entry name" value="K_chnl_volt-dep_Kv4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004055">
        <property type="entry name" value="K_chnl_volt-dep_Kv4.2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF265">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY D MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01517">
        <property type="entry name" value="KV42CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01497">
        <property type="entry name" value="SHALCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="19">
        <location>
            <begin position="62"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="272" mass="31362" checksum="7ED8DD14CDDD1228" modified="2019-05-08" version="1">SRHLAGRLPSLAFAGAGRRSSRLENNNLQPQQQPPLLSQLGEAWPTHLRAAPRQERKRSQDSLIVLNVSGTQFQTWQDTLERYPDTLLGSSERDFFYHPETQQYFFDRDPDIFRHILNFYRTGKLHYPRQECISAYDEELAFFGIIPEIIGDCCYEEYKDRRRENAERLQDDADQDHAAECSLPSMTTRERMWRAFENPHTSTLALVFYYVTGFFIAISVIANVVETVPCGVNPGRIKELPCGERYAVAFFCLDTACLRIPSRGRCLPAAWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPL8</accession>
    <name>A0A452IPL8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enhancer of mRNA-decapping protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat EDC4 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000029848"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033891.1">
        <property type="protein sequence ID" value="ENSGAGP00000029848.1"/>
        <property type="gene ID" value="ENSGAGG00000021550.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000033898.1">
        <property type="protein sequence ID" value="ENSGAGP00000029855.1"/>
        <property type="gene ID" value="ENSGAGG00000021550.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.100:FF:000001">
        <property type="entry name" value="Enhancer of mRNA-decapping protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000138">
        <property type="entry name" value="Enhancer of mRNA-decapping protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.100">
        <property type="entry name" value="conserved c-terminal region of ge- 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045152">
        <property type="entry name" value="EDC4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049404">
        <property type="entry name" value="EDC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044938">
        <property type="entry name" value="EDC4_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032401">
        <property type="entry name" value="EDC4_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598:SF5">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21289">
        <property type="entry name" value="EDC4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16529">
        <property type="entry name" value="Ge1_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Enhancer of mRNA-decapping protein 4 WD40 repeat region" evidence="13">
        <location>
            <begin position="112"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="291"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Enhancer of mRNA-decapping protein 4 C-terminal" evidence="14">
        <location>
            <begin position="1269"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="628"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="814"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="947"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="654"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="814"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="833"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF21289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1401" mass="153572" checksum="195155058BED9D0A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPT2</accession>
    <name>A0A452IPT2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IPT2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029917"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033975.1">
        <property type="protein sequence ID" value="ENSGAGP00000029917.1"/>
        <property type="gene ID" value="ENSGAGG00000021582.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02642">
        <property type="entry name" value="R3H_encore_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.50:FF:000001">
        <property type="entry name" value="R3H domain-containing protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051937">
        <property type="entry name" value="R3H_domain_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024771">
        <property type="entry name" value="SUZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672:SF14">
        <property type="entry name" value="CAMP-REGULATED PHOSPHOPROTEIN 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672">
        <property type="entry name" value="CAMP-REGULATED PHOSPHOPROTEIN 21 RELATED R3H DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12752">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51673">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="3">
        <location>
            <begin position="245"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="SUZ" evidence="4">
        <location>
            <begin position="309"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="102"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="176"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="318"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="412"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="565"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="666"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="420"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="432"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="442"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="482"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="521"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="591"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="616"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="666"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="693"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="729"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="923" mass="102730" checksum="B508886E71AEC401" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IR28</accession>
    <name>A0A452IR28_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000030318"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034411.1">
        <property type="protein sequence ID" value="ENSGAGP00000030318.1"/>
        <property type="gene ID" value="ENSGAGG00000021854.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040010">
        <property type="entry name" value="ZN608/ZN609"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21564">
        <property type="entry name" value="BRAKELESS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21564:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN 609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="495"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="47"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="351"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="518"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="695"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="796"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1002"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1039"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1108"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1155"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1271"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="392"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="519"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="546"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="623"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="647"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="747"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="827"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="854"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="897"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="925"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="951"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1010"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1155"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1197"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1287"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1330"/>
            <end position="1348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1413" mass="151019" checksum="E2180A030CB528F0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ISS3</accession>
    <name>A0A452ISS3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ISS3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031080"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035237.1">
        <property type="protein sequence ID" value="ENSGAGP00000031080.1"/>
        <property type="gene ID" value="ENSGAGG00000022328.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19482">
        <property type="entry name" value="RecA-like_Thep1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00796">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004948">
        <property type="entry name" value="Nuc-triphosphatase_THEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010248">
        <property type="entry name" value="PRK13695.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43146">
        <property type="entry name" value="CANCER-RELATED NUCLEOSIDE-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43146:SF1">
        <property type="entry name" value="CANCER-RELATED NUCLEOSIDE-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03266">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="190" mass="20894" checksum="A3FDD39EAEA581B2" modified="2019-05-08" version="1">MAKHVFLTGPPGIGKTTLIQKATEALKSSGVPIDGFYTEEVREGGRRIGFDVVTLSGRRGTLSRLGSDSSTARREYRVGQYVVDLVSFEHLVLPVLRNVDLGSDTGKKVHVIDEVGKMELFSQSFIQVVRQTLTASGTTILGTIPIPKGKPLGLVEEIRSRKDVKVFNVTKENRDNILQDIVTTVQNCRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITI0</accession>
    <name>A0A452ITI0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. ABHD16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ITI0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031259"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035456.1">
        <property type="protein sequence ID" value="ENSGAGP00000031259.1"/>
        <property type="gene ID" value="ENSGAGG00000022467.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052651">
        <property type="term" value="P:monoacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006660">
        <property type="term" value="P:phosphatidylserine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000074">
        <property type="entry name" value="Abhydrolase domain containing 16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF37">
        <property type="entry name" value="PROTEIN ABHD16B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="5">
        <location>
            <begin position="164"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="448" mass="50931" checksum="314FA2E74EC0A391" modified="2019-05-08" version="1">MCIICFTKALGHVFKTYLMASYNFEFRNWPVDFRWDEAGSKDSSPGHRAASQTTARKGLLKSAPHGGPRTTQCNYLRKMKQLPRQLVCYMVAHSLGRWLLYPGSLYLMQKVLLPLLVKGQARLLEEFHGKRAKLVARDGNEIDTMFMDRRKSSGMEEEQRGKQLVICCEGNVGFYEVGCLSTPLEAGYSALGWNHPGYAGSTGLPFPQNDANAVDVVIQYAICRLGFQVQDIILYGWSLGGYTATWAAKTYPELGALVLDATFDDLLPLALKVMPKSWNKLVVQTVRQHFNLNVAEQLCSYPGPVLLIRRTEDEVTTTRISTADQLADIRSNRGNELLLQLLKYRYPEVISWEGEAAIHHWLGASNPRQEEAVCRRFKVDDDWCVSKLQGYKSHLGGEDSFPWRVGKDVTPRRRQQLALFLARKHMKNVEATHWSLLLPNEFQMPWKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITK0</accession>
    <name>A0A452ITK0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small ribosomal subunit protein uS17m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">28S ribosomal protein S17, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ITK0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031208"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035395.1">
        <property type="protein sequence ID" value="ENSGAGP00000031208.1"/>
        <property type="gene ID" value="ENSGAGG00000022429.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000137">
        <property type="entry name" value="28S ribosomal protein S17, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039193">
        <property type="entry name" value="Ribosomal_uS17m_metazoa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24088">
        <property type="entry name" value="28S RIBOSOMAL PROTEIN S17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24088:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="136" mass="15199" checksum="F6A78D89ABDEB5AD" modified="2019-05-08" version="1">MSGLRGAVHAKWIIGKVIGTKMHKTAKVRVTRLVLDPYLLKFFNKRKTYFAHDPLQQCAVGDIVLLKALPERRTKNVKHELAEIVFKVGNVIDPVTGKACAGTRFLESLTDSESLTEADTTYLSEKLQELKVSSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITP7</accession>
    <name>A0A452ITP7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ITP7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031483"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035707.1">
        <property type="protein sequence ID" value="ENSGAGP00000031483.1"/>
        <property type="gene ID" value="ENSGAGG00000022612.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="4">
        <location>
            <begin position="78"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="257" mass="28500" checksum="0C4F194724665052" modified="2019-05-08" version="1">MAAPMAGRLAALGRRLAPPARAWLPGLTQVRWSRYGPSYLEPEVNKEVYRKPLEGMSEEEKTEWELKTLQPIKATASNVTSSVFSDPTISKFTNMIMKGGNKVLARSIVTQTLEAIKRKQIEKYHQASEEERESIECNPYIIFHQALKNCQPIIGLCNVQKGGKTYQVRVFADRSGENLAPRTAAAEAGKAGAQAWNANARWASFMQSPLHQESALRDPSWQDQAENPSCCSYFSASLGSVIATRRILSIANLRVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IU52</accession>
    <name>A0A452IU52_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mesogenin-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IU52"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031587"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035823.1">
        <property type="protein sequence ID" value="ENSGAGP00000031587.1"/>
        <property type="gene ID" value="ENSGAGG00000022684.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18939">
        <property type="entry name" value="bHLH_TS_Msgn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000056">
        <property type="entry name" value="mesogenin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040259">
        <property type="entry name" value="Mesogenin/MesP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20937">
        <property type="entry name" value="IP14615P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20937:SF4">
        <property type="entry name" value="MESOGENIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="99"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="163" mass="18143" checksum="CAE9294B74B869D3" modified="2019-05-08" version="1">MEDGLGTENSVFLSSWDWKSNAGAYELNQISSPHSLSPSPSFESYSSSPCPAVIEMPYNNSSSGGSLIGYSLMDFPSTYLPNAGQAKAQKGTKARMSAQRRRKASEREKLRMRTLADALHTLRNYLPPVYSQRGQPLTKIQTLKYTIKYISELTDLLNNVKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUN6</accession>
    <name>A0A452IUN6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Barrier-to-autointegration factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Homodimer. Heterodimerizes with BANF2. Interacts with ANKLE2/LEM4, leading to decreased phosphorylation by VRK1 and promoting dephosphorylation by protein phosphatase 2A (PP2A). Binds non-specifically to double-stranded DNA, and is found as a hexamer or dodecamer upon DNA binding. Binds to LEM domain-containing nuclear proteins such as LEMD3/MAN1, TMPO/LAP2 and EMD (emerin). Interacts with ANKLE1 (via LEM domain); the interaction may favor BANF1 dimerization. Interacts with CRX and LMNA (lamin-A). Binds linker histone H1.1 and core histones H3. Interacts with LEMD2 (via LEM domain). Interacts with PARP1; interaction takes place in response to oxidative DNA damage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the BAF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IUN6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031717"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035968.1">
        <property type="protein sequence ID" value="ENSGAGP00000031717.1"/>
        <property type="gene ID" value="ENSGAGG00000022779.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.40:FF:000001">
        <property type="entry name" value="Barrier-to-autointegration factor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.40">
        <property type="entry name" value="Barrier-to-autointegration factor, BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051387">
        <property type="entry name" value="BAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004122">
        <property type="entry name" value="BAF_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036617">
        <property type="entry name" value="BAF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47507">
        <property type="entry name" value="BARRIER TO AUTOINTEGRATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47507:SF7">
        <property type="entry name" value="BARRIER-TO-AUTOINTEGRATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02961">
        <property type="entry name" value="SAM_BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01023">
        <property type="entry name" value="BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47798">
        <property type="entry name" value="Barrier-to-autointegration factor, BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="90" mass="9985" checksum="018EDCD9F9FC3054" modified="2019-05-08" version="1">MSSTSQKHRDFVAEPMGEKPVGTLAGIGDVLGKKLADKGFDKAYVVLGQFLVLKKDEELFREWLKDTCGANAKQSRDCSGCLREWCDAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IVK2</accession>
    <name>A0A452IVK2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IVK2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032109"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036406.1">
        <property type="protein sequence ID" value="ENSGAGP00000032109.1"/>
        <property type="gene ID" value="ENSGAGG00000023007.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17219">
        <property type="entry name" value="RA_RASSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21891">
        <property type="entry name" value="SARAH_RASSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000048">
        <property type="entry name" value="Ras association domain family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033614">
        <property type="entry name" value="RASSF1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011524">
        <property type="entry name" value="SARAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22738:SF8">
        <property type="entry name" value="RAS ASSOCIATION DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22738">
        <property type="entry name" value="RASSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16517">
        <property type="entry name" value="Nore1-SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50951">
        <property type="entry name" value="SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Ras-associating" evidence="10">
        <location>
            <begin position="110"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="SARAH" evidence="11">
        <location>
            <begin position="198"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="249" mass="28868" checksum="F65370E3D13CE56E" modified="2019-05-08" version="1">MSSGYSSLEEEESEEFFTARTSFFGRPPAKARPELQDVQKERETHTYLSKDEVKEKIQNYNSSVTDKLKMTLNSNGTYTGFIKVQMELYRPITVQTSVNPGEHAHNNNETSFYLPPGYVNKLHISSINTVREVIEALLKKFLVFDNPAKFALYKRCHKEDQVYTCKLSDREHPLYLRLVAGPSTETLSFVLREHETGEVMWEAFSLPELQNFLRILDKEEDEQLQILKRRYAAYKDKLEEALGGVWKPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYF9</accession>
    <name>A0A452IYF9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IYF9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033124"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037546.1">
        <property type="protein sequence ID" value="ENSGAGP00000033124.1"/>
        <property type="gene ID" value="ENSGAGG00000023645.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="86" mass="9440" checksum="D3FE32351DFBE9D5" modified="2019-05-08" version="1">PGICRGRRRRMAAAGPVCKCPRTRLPVGGASALCVFVLCWLYIFPVYRLPDEKEVVQGVLLQQGKAWRSNQTASLINNDSSFSCGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYR6</accession>
    <name>A0A452IYR6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphatidylinositol-3,5-bisphosphate 3-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Phosphatidylinositol-3,5-bisphosphate 3-phosphatase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.95" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452IYR6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033209"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037633.1">
        <property type="protein sequence ID" value="ENSGAGP00000033209.1"/>
        <property type="gene ID" value="ENSGAGG00000023677.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061952">
        <property type="term" value="P:midbody abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15732">
        <property type="entry name" value="FYVE_MTMR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14586">
        <property type="entry name" value="PTP-MTMR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000073">
        <property type="entry name" value="myotubularin-related protein 4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046352">
        <property type="entry name" value="MTMR3_PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF66">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="17">
        <location>
            <begin position="166"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="16">
        <location>
            <begin position="1128"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="601"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="758"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1005"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1044"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="625"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="760"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1009"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="11">
        <location>
            <position position="424"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="337"/>
            <end position="340"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="362"/>
            <end position="363"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="424"/>
            <end position="430"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1216" mass="136873" checksum="02A9700D21D036FD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1M7</accession>
    <name>A0A452J1M7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J1M7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034204"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038730.1">
        <property type="protein sequence ID" value="ENSGAGP00000034204.1"/>
        <property type="gene ID" value="ENSGAGG00000024296.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF81">
        <property type="entry name" value="GRANZYME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="21"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="224" mass="25494" checksum="5359AEE29EA5F563" modified="2019-05-08" version="1">ITEPVSPLLVFTPQLLEQGEIIRSREAKVHSRCYMAYLEIQHGKVTKKCGWFLVKPNLVLMDAHGKGDEITVTLGAHNISKPEWSQQKIPVRRQVPHLQYDKKTLNIVIMLHVPIFLFSLLPGSTCRVAGWDRTVIKNNMCELYPGYNRPMMLCGGSLALRNCLIRKAVFFTQKIFLGDSGDPLVCEGVVQGIASYVVNFYKPSVFTRLSKFIPWIQKILQNQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J216</accession>
    <name>A0A452J216_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Laminin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J216"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034335"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038877.1">
        <property type="protein sequence ID" value="ENSGAGP00000034335.1"/>
        <property type="gene ID" value="ENSGAGG00000024402.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF270">
        <property type="entry name" value="LAMININ SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019476971" description="Laminin N-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="33"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="4">
        <location>
            <begin position="44"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="219" mass="23704" checksum="F052953F171616F9" modified="2019-05-08" version="1">ARYGLQAAPAAGMRRRALPLLLLLELAGRCVAAMDECADEASGRPQRCMPEFVNAAFNVTVVATNTCGSPAEEYCVQTGVTGVTKSCHLCDAAQPHLQHGAAFLTDYNQADTTWWQSQTMLAGVQYPSAINLTLHLGKSAGLLAPPVPALGHSPSRRGAEGLSWRDRYPPPLLPLRNSGDTCMPNRNFIRMPSPIIPFCLHCIGVPVLDDLYLSLWNLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J327</accession>
    <name>A0A452J327_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J327"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034831"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039426.1">
        <property type="protein sequence ID" value="ENSGAGP00000034831.1"/>
        <property type="gene ID" value="ENSGAGG00000024744.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046333">
        <property type="entry name" value="HXA10/ABDB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45874">
        <property type="entry name" value="HOMEOBOX PROTEIN ABDOMINAL-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45874:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="130" mass="13685" checksum="46D4EC9ACB9969C0" modified="2019-05-08" version="1">MSCSESPAANSFLVDSLISSGRGEGGGGGGGGGGGGYYPNSSVFLPQAADLSYGLQSCGLFPVLSKRNEGAAQNMVPTSHAYMSGMEVWLEPPRSCRLEEPETQQATSFTSQMERRIRAACRAGKKRRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J445</accession>
    <name>A0A452J445_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Chemerin-like receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Chemokine-like receptor 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the chemokine-like receptor (CMKLR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J445"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035201"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039859.1">
        <property type="protein sequence ID" value="ENSGAGP00000035201.1"/>
        <property type="gene ID" value="ENSGAGG00000025021.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004875">
        <property type="term" value="F:complement receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15116">
        <property type="entry name" value="7tmA_CMKLR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000034">
        <property type="entry name" value="G-protein coupled receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002258">
        <property type="entry name" value="CML1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000826">
        <property type="entry name" value="Formyl_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225:SF49">
        <property type="entry name" value="CHEMERIN-LIKE RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225">
        <property type="entry name" value="CHEMOTACTIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01126">
        <property type="entry name" value="DEZORPHANR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="213"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="56"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="357" mass="40339" checksum="B938646BB3D67E5F" modified="2019-05-08" version="1">METYNYSYDYLVDYNYDYYNSTTIRVKQEPSHNAGDIARILSVVVYSLTCLLGILGNGLVITIITFKMKKSINAIWFLNLAVADFLFNVFLPLNIAYTAMNYHWVFGAAMCKLNSFLLNLNLYSSVLLLTTISLDRCVSVVLPVWAQNHRTTTLACLVCVVDWVLGLVMSSPSLVFRDTATHEGNVICFSNYTLSGNKSDHPLGMRRHQTVNLVRFLTGFVIPVTIITVCYMIIVVKLKRNRLAKSKKPFKIIVTIIVTFFLCWTPYHVLNLLETQHEQVSHVVFKIGIPITTALAAASSCMNPILYVFMGQDFKKFKVTILSRLLNALSEDATHSTLAQRSFSKMSSMTEKDTTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J558</accession>
    <name>A0A452J558_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial import inner membrane translocase subunit TIM14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DnaJ homolog subfamily C member 19</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Mitochondrial co-chaperone which forms a complex with prohibitins to regulate cardiolipin remodeling. May be a component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. May act as a co-chaperone that stimulate the ATP-dependent activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with PHB2; the interaction associates DNAJC19 with the prohibitin complex. Interacts with TIMM16/PAM16. May be a component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TIM14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J558"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035590"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040304.1">
        <property type="protein sequence ID" value="ENSGAGP00000035590.1"/>
        <property type="gene ID" value="ENSGAGG00000025289.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000001">
        <property type="entry name" value="Import inner membrane translocase subunit tim14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763:SF56">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="J" evidence="16">
        <location>
            <begin position="61"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="115" mass="12356" checksum="EF74FA9369FFEC05" modified="2019-05-08" version="1">MATTVIAVGLTIAAAGFAGRYALQAMKQMEPQVKQALQSLPKSAFSGYYRGGFDPKMTKREAALILGVSPTANKGKIREAHRRIMLLNHPDKGGSPYVAAKINEAKDLLEGQAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFB7</accession>
    <name>A0A452GFB7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Integrator subunit 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GFB7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000233"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000265.1">
        <property type="protein sequence ID" value="ENSGAGP00000000233.1"/>
        <property type="gene ID" value="ENSGAGG00000000206.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056518">
        <property type="entry name" value="HEAT_Ints3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045334">
        <property type="entry name" value="INTS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019333">
        <property type="entry name" value="INTS3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13587">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13587:SF7">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24566">
        <property type="entry name" value="HEAT_Ints3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10189">
        <property type="entry name" value="Ints3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrator complex subunit 3 N-terminal" evidence="6">
        <location>
            <begin position="64"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Ints3-like C-terminal" evidence="7">
        <location>
            <begin position="561"/>
            <end position="943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1022" mass="116915" checksum="A9CFD28ACC8FE126" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFH3</accession>
    <name>A0A452GFH3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nuclear factor NF-kappa-B p105 subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Constitutes the active form, which associates with RELA/p65 to form the NF-kappa-B p65-p50 complex to form a transcription factor. Together with RELA/p65, binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions.</text>
    </comment>
    <comment type="function">
        <text evidence="21">P105 is the precursor of the active p50 subunit (Nuclear factor NF-kappa-B p50 subunit) of the nuclear factor NF-kappa-B. Acts as a cytoplasmic retention of attached NF-kappa-B proteins by p105.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GFH3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000250"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000286.1">
        <property type="protein sequence ID" value="ENSGAGP00000000250.1"/>
        <property type="gene ID" value="ENSGAGG00000000222.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035525">
        <property type="term" value="C:NF-kappaB p50/p65 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071356">
        <property type="term" value="P:cellular response to tumor necrosis factor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08797">
        <property type="entry name" value="Death_NFkB1_p105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01177">
        <property type="entry name" value="IPT_NFkappaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07935">
        <property type="entry name" value="RHD-n_NFkB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000046">
        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000018">
        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000103">
        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000004">
        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033926">
        <property type="entry name" value="IPT_NFkappaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047096">
        <property type="entry name" value="NF-kB_p105_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030503">
        <property type="entry name" value="NF-kB_p105_RHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000451">
        <property type="entry name" value="NFkB/Dor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030492">
        <property type="entry name" value="RHD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169:SF9">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B P105 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00057">
        <property type="entry name" value="NFKBTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01204">
        <property type="entry name" value="REL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="12 22" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="17" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="18" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0702">S-nitrosylation</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RHD" evidence="23">
        <location>
            <begin position="44"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="22">
        <location>
            <begin position="542"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="22">
        <location>
            <begin position="581"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="22">
        <location>
            <begin position="614"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="22">
        <location>
            <begin position="650"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="22">
        <location>
            <begin position="684"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="22">
        <location>
            <begin position="718"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00032626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00056174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="981" mass="107616" checksum="845943EB17430F66" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGL4</accession>
    <name>A0A452GGL4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GGL4"/>
    <dbReference type="SMR" id="A0A452GGL4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000633"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000711.1">
        <property type="protein sequence ID" value="ENSGAGP00000000633.1"/>
        <property type="gene ID" value="ENSGAGG00000000502.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000000800.1">
        <property type="protein sequence ID" value="ENSGAGP00000000710.1"/>
        <property type="gene ID" value="ENSGAGG00000000581.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000004">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="12 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="7"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="16">
        <location>
            <begin position="92"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="129" mass="13988" checksum="358AE20D1C531724" modified="2019-05-08" version="1">MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYMAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKKTESHKAKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHS0</accession>
    <name>A0A452GHS0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GHS0"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001300.1">
        <property type="protein sequence ID" value="ENSGAGP00000001161.1"/>
        <property type="gene ID" value="ENSGAGG00000000936.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="86" mass="9860" checksum="051F00E7A6471A30" modified="2019-05-08" version="1">MFPLIPNLFMRIPLQCIFFVCPSKLGTVGHACHPSYLGVETVTHRKQDALSTFLLKERAKARDVTWSQEMIQEAVLKLNPARIFQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHX8</accession>
    <name>A0A452GHX8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GHX8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001225"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001377.1">
        <property type="protein sequence ID" value="ENSGAGP00000001225.1"/>
        <property type="gene ID" value="ENSGAGG00000000984.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058586">
        <property type="entry name" value="Ajm-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038825">
        <property type="entry name" value="Apical_junction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21517">
        <property type="entry name" value="APICAL JUNCTION COMPONENT 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21517:SF3">
        <property type="entry name" value="APICAL JUNCTION COMPONENT 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26649">
        <property type="entry name" value="Ajm-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="6">
        <location>
            <begin position="798"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="77"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="635"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="718"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="111"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="636"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1098" mass="123873" checksum="991695F28B278E38" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIJ6</accession>
    <name>A0A452GIJ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lysophosphatidylserine lipase ABHD12</fullName>
            <ecNumber evidence="4">3.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">2-arachidonoylglycerol hydrolase ABHD12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Abhydrolase domain-containing protein 12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Monoacylglycerol lipase ABHD12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Oxidized phosphatidylserine lipase ABHD12</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Lysophosphatidylserine (LPS) lipase that mediates the hydrolysis of lysophosphatidylserine, a class of signaling lipids that regulates immunological and neurological processes. Represents a major lysophosphatidylserine lipase in the brain, thereby playing a key role in the central nervous system. Also able to hydrolyze oxidized phosphatidylserine; oxidized phosphatidylserine is produced in response to severe inflammatory stress and constitutes a proapoptotic 'eat me' signal. Also has monoacylglycerol (MAG) lipase activity: hydrolyzes 2-arachidonoylglycerol (2-AG), thereby acting as a regulator of endocannabinoid signaling pathways. Has a strong preference for very-long-chain lipid substrates; substrate specificity is likely due to improved catalysis and not improved substrate binding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes glycerol monoesters of long-chain fatty acids.</text>
            <dbReference type="EC" id="3.1.1.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the serine esterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.23" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452GIJ6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001482"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001690.1">
        <property type="protein sequence ID" value="ENSGAGP00000001482.1"/>
        <property type="gene ID" value="ENSGAGG00000001188.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052651">
        <property type="term" value="P:monoacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006660">
        <property type="term" value="P:phosphatidylserine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000069">
        <property type="entry name" value="monoacylglycerol lipase ABHD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF61">
        <property type="entry name" value="LYSOPHOSPHATIDYLSERINE LIPASE ABHD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="20">
        <location>
            <begin position="293"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="135"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="522" mass="58337" checksum="D8A1025E1BDCFE99" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKM6</accession>
    <name>A0A452GKM6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000002204"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002521.1">
        <property type="protein sequence ID" value="ENSGAGP00000002204.1"/>
        <property type="gene ID" value="ENSGAGG00000001791.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029073">
        <property type="entry name" value="DUF4661"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14307">
        <property type="entry name" value="C6ORF47 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14307:SF0">
        <property type="entry name" value="SI:CH73-25F10.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15576">
        <property type="entry name" value="DUF4661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="278" mass="30264" checksum="66964D1CD6484E0A" modified="2019-05-08" version="1">PSSPEAPPPAQSXAGERTQESWLPAPPCRGKNPGVRAPQRAKAWLTKLRWWGPEAGKEGGLTEAPRKSQRWGAQRLLALIGWGRAEDPGPAPGESLFSRVSQVPQYLSPHQPPADVPEHFQICFNFSRHLFDLCVVTLLCACSPAFRLLLDILGFRGPLKVWLHGLATFLVTTYGMYLALWLVQKYLLQFACLYGFLQTLVLCVNRTRSWGLRVKSEGHQQSWGGQGWASRGWGSRVRGTSGAGGGQGWASRGCGSGVRGTRRAGEPRAGLAGSAGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKP8</accession>
    <name>A0A452GKP8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tissue factor pathway inhibitor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Finds in a complex with ABCB1, TFPI2 and PPP2R3C; leading to the dephosphorylation of ABCB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GKP8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002173"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002480.1">
        <property type="protein sequence ID" value="ENSGAGP00000002173.1"/>
        <property type="gene ID" value="ENSGAGG00000001756.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22615">
        <property type="entry name" value="Kunitz_TFPI1_TFPI2_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22616">
        <property type="entry name" value="Kunitz_TFPI2_1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22617">
        <property type="entry name" value="Kunitz_TFPI2_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000004">
        <property type="entry name" value="Tissue factor pathway inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000011">
        <property type="entry name" value="Tissue factor pathway inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000018">
        <property type="entry name" value="Tissue factor pathway inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008296">
        <property type="entry name" value="TFPI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001620">
        <property type="entry name" value="TFPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="3 13" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 13" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018824942" description="Tissue factor pathway inhibitor" evidence="13">
        <location>
            <begin position="31"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="14">
        <location>
            <begin position="43"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="14">
        <location>
            <begin position="103"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="14">
        <location>
            <begin position="163"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="241" mass="26949" checksum="54A23BC4CE3B2219" modified="2019-05-08" version="1">MPGLAPLQAKAMDSSARLGWLLLLLGQVLGAPQAPAAPNQEVCLLSPEEGPCRALIPRWHYDRYTQTCQEFTYGGCEGNANNFRSLESCEKSCWMIRKVPKICRLEADPGPCRGYLKRYFFNLTSMKCEKFIYGGCYGNDNNFQDEDSCVDYCLSEKTGPSLCYSPKDEGLCSSSVTRYYYNSKSQSCEEFSYTGCGGNANNFVTKKDCHSICKKAGSKKPSFNKSRNKLPKTLRKLQGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLM1</accession>
    <name>A0A452GLM1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kelch-like protein 15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Dimerization does not affect PPP2R5B-binding, but is required for its proteasomal degradation. Interacts with CUL3. Directly interacts with PPP2R5B; this interaction leads to PPP2R5B proteasomal degradation. Interacts with RBBP8/CtIP; this interaction leads to RBBP8 proteasomal degradation. Interacts with PACMP micropeptide; interaction prevents ubiquitination and degradation of RBBP8/CtIP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GLM1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002699"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003094.1">
        <property type="protein sequence ID" value="ENSGAGP00000002699.1"/>
        <property type="gene ID" value="ENSGAGG00000002169.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071630">
        <property type="term" value="P:nuclear protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18454">
        <property type="entry name" value="BACK_KLHL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18244">
        <property type="entry name" value="BTB_POZ_KLHL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000087">
        <property type="entry name" value="Kelch-like family member 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000009">
        <property type="entry name" value="Kelch-like protein 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000014">
        <property type="entry name" value="Kelch-like protein 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030597">
        <property type="entry name" value="BTB_POZ_KLHL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047030">
        <property type="entry name" value="KLHL15_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF12">
        <property type="entry name" value="KELCH-LIKE PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="9">
        <location>
            <begin position="31"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="604" mass="69738" checksum="08F0FE164063086E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLP4</accession>
    <name>A0A452GLP4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Dynein axonemal heavy chain 17</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GLP4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002595"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002968.1">
        <property type="protein sequence ID" value="ENSGAGP00000002595.1"/>
        <property type="gene ID" value="ENSGAGG00000002068.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000002">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000411">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000002">
        <property type="entry name" value="Dynein heavy chain 9, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961:SF16">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 1, AXONEMAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="13">
        <location>
            <begin position="251"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="14">
        <location>
            <begin position="412"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="15">
        <location>
            <begin position="556"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="851" mass="97585" checksum="7FAE3E5E27706CCD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMZ6</accession>
    <name>A0A452GMZ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FYN-binding protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Activation-dependent, raft-recruited ADAP-like phosphoprotein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Adapter protein that plays a role in T-cell receptor (TCR)-mediated activation of signaling pathways. Required for T-cell activation and integrin-mediated T-cell adhesion in response to TCR stimulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with SKAP1, LCK and FYN. The phosphorylated form interacts with LCP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane raft</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMZ6"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003716.1">
        <property type="protein sequence ID" value="ENSGAGP00000003220.1"/>
        <property type="gene ID" value="ENSGAGG00000002605.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000220">
        <property type="entry name" value="FYN binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043443">
        <property type="entry name" value="FYB1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029294">
        <property type="entry name" value="hSH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16830:SF1">
        <property type="entry name" value="FYN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16830">
        <property type="entry name" value="SH2 CONTAINING ADAPTOR PRAM-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14603">
        <property type="entry name" value="hSH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="683"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="17"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="217"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="279"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="425"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="510"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="523"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00079345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="742" mass="83525" checksum="75083E785DDA7053" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNJ0</accession>
    <name>A0A452GNJ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calmodulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GNJ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003443"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003971.1">
        <property type="protein sequence ID" value="ENSGAGP00000003443.1"/>
        <property type="gene ID" value="ENSGAGG00000002770.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="79" mass="8931" checksum="920B30EAA7BED57F" modified="2019-05-08" version="1">MADQLTEGQIAEFKEAFSLFDKDGDGTITTSELGTVMRSLGQNPTEAELQDMIDHISLACLCEFMWDWVKIWTKINITT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPU1</accession>
    <name>A0A452GPU1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 106</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Zinc finger protein 474</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">RNA-binding protein. Specifically binds to 5'-GGGGCC-3' sequence repeats in RNA. Essential for maintenance of peripheral motor neuron and skeletal muscle function. Required for normal expression and/or alternative splicing of a number of genes in spinal cord and skeletal muscle, including the neurite outgrowth inhibitor RTN4. Also contributes to normal mitochondrial respiratory function in motor neurons, via an unknown mechanism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with KNOP1. Interacts with TARDBP and NUP107. Interacts (via N-terminus) with RBM39. Interacts with the SH3 domains of FYN and GRB2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000003707"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004288.1">
        <property type="protein sequence ID" value="ENSGAGP00000003707.1"/>
        <property type="gene ID" value="ENSGAGG00000003008.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000195">
        <property type="entry name" value="Zinc finger protein 106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000114">
        <property type="entry name" value="zinc finger protein 106 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042622">
        <property type="entry name" value="Znf106"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14435">
        <property type="entry name" value="ZINC FINGER PROTEIN 106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14435:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN 106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0853">WD repeat</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="1840"/>
            <end position="1863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="330"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="559"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1004"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1178"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1283"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1353"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="364"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="379"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="581"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1004"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1049"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1178"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1200"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1285"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1298"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1371"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1426"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1469"/>
            <end position="1483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1908" mass="213454" checksum="A778C21A8C534AFE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPU8</accession>
    <name>A0A452GPU8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carbohydrate kinase FGGY C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPU8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003932"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004577.1">
        <property type="protein sequence ID" value="ENSGAGP00000003932.1"/>
        <property type="gene ID" value="ENSGAGG00000001932.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000033">
        <property type="entry name" value="glycerol kinase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF69">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="6">
        <location>
            <begin position="58"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="317" mass="33978" checksum="0608698E22190F4C" modified="2019-05-08" version="1">MEILPKVRSSSEIYGLMKSGALTGVPISGCLGDQSAALVGQMCFKDGQAKNTSSLSSIPILSEHGLLTTVAYKLGRDKPVCYALEGSVAIAGAVVRWLRDNLGIVKSSSEVEKLAAEVGTSYGCYFVPAFSGLYAPYWEPSARGIICGLTQFTNKNHIAFAALEAVCFQTREILDAMNKDCGIPLSELQVDGGMTNNKILMQLQADILCIQVVKPSMPETTALGAAMAAGAAEGVGVWSLNPGDLTAVTWERFEPQINPEESEYRYARWKKAVMKSMGWEISEPQANGDSSIFSSLPLGFYIVSSMIMLIGAKYISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GR68</accession>
    <name>A0A452GR68_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hsp90 co-chaperone Cdc37-like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Co-chaperone that binds to numerous proteins and promotes their interaction with Hsp70 and Hsp90.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC37 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GR68"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004448"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005216.1">
        <property type="protein sequence ID" value="ENSGAGP00000004448.1"/>
        <property type="gene ID" value="ENSGAGG00000003684.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.610:FF:000001">
        <property type="entry name" value="Hsp90 co-chaperone Cdc37-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.610">
        <property type="entry name" value="Cdc37, Hsp90 binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004918">
        <property type="entry name" value="Cdc37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013874">
        <property type="entry name" value="Cdc37_Hsp90-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038189">
        <property type="entry name" value="Cdc37_Hsp90-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800">
        <property type="entry name" value="CDC37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800:SF2">
        <property type="entry name" value="HSP90 CO-CHAPERONE CDC37-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08565">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01070">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101391">
        <property type="entry name" value="Hsp90 co-chaperone CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc37 Hsp90 binding" evidence="9">
        <location>
            <begin position="119"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="73"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="324" mass="37421" checksum="403C59A9FD03BFBC" modified="2019-05-08" version="1">MALWPPRSRGLRAGEGQEPDPPCAPRPPDPQIYGHGIELACQKQKEFVKSSVECKWNLAEAQQKLGSLALHNSESLDQEHAKAQTDASELRQREEEWRQKEEALIQRETQNLWNTDSVSKDVFNKSFINQKKRKEIEEEDKSKSFLQKHEQKIRYFGMLSRWDDSQRFLSDHPYLVCEDTAKYLILWCFHLEAEQKRALMEQVAHQAVVMQFITEMAKSCNVDPRGCFRLFFQRAKAEEEGYFEAFKNELEGFKSRVKISSQSQSFQAMSVQNPIAHTGLSSLGVLEFLPQTADSLQSCVNTGLFNLNSLVHRDDEEPKMMDTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GS60</accession>
    <name>A0A452GS60_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GS60"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004831"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005650.1">
        <property type="protein sequence ID" value="ENSGAGP00000004831.1"/>
        <property type="gene ID" value="ENSGAGG00000003964.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14388:SF7">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14388">
        <property type="entry name" value="T CELL-SPECIFIC ADAPTER PROTEIN TSAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0727">SH2 domain</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="192"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="142"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="199"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="381" mass="45309" checksum="58421CB5886AB514" modified="2019-05-08" version="1">MLQQILHDMYIDPELLAELNDEQKHILFYKMREEQLRRWREKEEKEQREEAALKRTARFKQSKRVQWLHGSDGEIWVWIMGDSPGDKPYEQISEELMAERARQQAQKEAEELWKQKEAEITKKFRDAMAKEKAKIVAEKWKLETEDRKAAKLMEEKIQEELKKREEEERQRGEEQIRQQEELRAKELYLSLKQAQQHSQHSEHKDQEWKEQLRRSKAADEERSYKACKARDEYRRQSLRAIQKGKVAGLSNLFKGTSLSNEPEIKLNSNNPSPLMTLTTPVRFVSWERPSRPISREILIRWFKEEQLPRHAGFERNTCAVAPWFHGNSSSLTREPPSLPAYQGINKLNVHMENHISTNHLLQARNEEIQLWTALVSLQVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSB3</accession>
    <name>A0A452GSB3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Dual serine/threonine and tyrosine protein kinase</fullName>
            <ecNumber evidence="5">2.7.12.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Dusty protein kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Receptor-interacting serine/threonine-protein kinase 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452GSB3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004880"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005703.1">
        <property type="protein sequence ID" value="ENSGAGP00000004880.1"/>
        <property type="gene ID" value="ENSGAGG00000004005.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044344">
        <property type="term" value="P:cellular response to fibroblast growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045743">
        <property type="term" value="P:positive regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13975">
        <property type="entry name" value="PKc_Dusty"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000244">
        <property type="entry name" value="Dual serine/threonine and tyrosine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051302">
        <property type="entry name" value="Dual_SerThr-Tyr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46392">
        <property type="entry name" value="DUAL SERINE/THREONINE AND TYROSINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46392:SF1">
        <property type="entry name" value="DUAL SERINE_THREONINE AND TYROSINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="654"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="58"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="412"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="24">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="683"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="931" mass="105422" checksum="C3CD4E61C623D9CB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSY5</accession>
    <name>A0A452GSY5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GSY5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005113"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005960.1">
        <property type="protein sequence ID" value="ENSGAGP00000005113.1"/>
        <property type="gene ID" value="ENSGAGG00000004159.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000006">
        <property type="entry name" value="uncharacterized protein C16orf52 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037663">
        <property type="entry name" value="Mosmo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31186">
        <property type="entry name" value="MODULATOR OF SMOOTHENED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31186:SF1">
        <property type="entry name" value="MODULATOR OF SMOOTHENED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18800">
        <property type="entry name" value="Atthog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019154189" evidence="2">
        <location>
            <begin position="27"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="167" mass="18249" checksum="77AE406F1FEA5DF1" modified="2019-05-08" version="1">MDKLTIISGCLFLAADIFAIASLANPDWINTGESAGALTVGLVRQCQTIHGRDRTCIPPRLPPEWVTTLFFIIMGIISLTVTCGLLVASHWRRQATKYARWIAFTGMILFCMAALIFPIGFYINEVGGQPYKLPNNTVVGSSYVLFVLSIFFTIVGLLFAGKVCLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTT9</accession>
    <name>A0A452GTT9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTT9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005462"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006358.1">
        <property type="protein sequence ID" value="ENSGAGP00000005462.1"/>
        <property type="gene ID" value="ENSGAGG00000004432.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18648">
        <property type="entry name" value="CD_Cbx6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000006">
        <property type="entry name" value="Chromobox protein homolog 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033773">
        <property type="entry name" value="CBX7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017984">
        <property type="entry name" value="Chromo_dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052458">
        <property type="entry name" value="PcG_PRC1-like_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389:SF4">
        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17218">
        <property type="entry name" value="CBX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00504">
        <property type="entry name" value="CHROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="7">
        <location>
            <begin position="11"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="271"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="324"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="275"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="403" mass="43446" checksum="52E2B362619E4A18" modified="2019-05-08" version="1">MELSAVGERVFAAESIIKRRIRKGRIEYLVKWKGWAIKYSTWEPEENILDSRLIAAFEQKERERELYGPKKRGPKPKTFLLKARAQAEALHIGDVHFSVKPGSSTSSPKLHSSAAVHRLKKDIRRCHRMSRRPLPRPDPQNSGGVGGGAGIRPPVSPFSETVRIINRKVKPREPKRSRIILNLKVIDKGGKAASGGGGLARPKIPSRNRVIGKSKKFSESILRTQIRHMKFGTFSLYNKPSAAPATSLEGKMEAGGSQGASCGLIIGSTPYDARSSSSSGCPSPTPHSSSDPDDSPPKLLPETLSPAIPDWRESEVLDLSIPPESAATSKRSPPGGCAGGQTPSSSLSSSDPEQEAGDWRPEMSPCSNVVVTDVTSNLLTVTIKEFCNAEDFEKVAAGGGGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUF2</accession>
    <name>A0A452GUF2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arylamine N-acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the arylamine N-acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.5" evidence="2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005391"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006276.1">
        <property type="protein sequence ID" value="ENSGAGP00000005391.1"/>
        <property type="gene ID" value="ENSGAGG00000004374.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004060">
        <property type="term" value="F:arylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2140.10">
        <property type="entry name" value="Arylamine N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.150">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF3">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01543">
        <property type="entry name" value="ANATRNSFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="323" mass="35522" checksum="8AAE6B867E178F85" modified="2019-05-08" version="1">AGNPKLGSKIPGFSPWLWVGSRGWWLEWGGPRARTPGFSAHSPRFAPQPGMDVGAYLSRIGYQGPKEPTLETLQGLHRCHLFSVPFESLSVHCGEPITLALPLLYDKIVRRQRGGFCYENNGLFLWLLRALGFCVVGLAGRVRNRFTGRLGPPRDHLVLLVRLGGQRFLCDVGFGDGFLEPLRLEPGLEQPQEGGTFRLSLAGGVWVLERLQAPGKGGQLLYTFTLEERELGDFAAMCCYHQQAPSSIFACKSFCSLPQPGGGRLTYMDPRLAAAXTPLQPHEIPALLQHTFGAQLSRTLVPKDQAIQPPPEGEEMEQLPSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUZ9</accession>
    <name>A0A452GUZ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytokine receptor-like factor 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May play a role in the negative regulation of cell cycle progression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytokine receptor-like factor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GUZ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005947"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006934.1">
        <property type="protein sequence ID" value="ENSGAGP00000005947.1"/>
        <property type="gene ID" value="ENSGAGG00000004835.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000573">
        <property type="entry name" value="Cytokine receptor-like factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="203"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="38"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00068086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="466" mass="52193" checksum="A0FE58763C06126C" modified="2019-05-08" version="1">MWLASGCEASPGGCCQQRVPTARAGSGMDPEGEALLLLQEARESVEAARSYRRELQQRLRGLQQARKQIRESAALTRDVLEQHFNDLKGTLWKLLDERLVLLRQEVDAIEQESIKPLDECQKLIEHGVSTADDLLQEGECVIHEVMGEQNEKLCNFTKKALHIQLDSLPEVPALVDVPCLSAQLDDCLLTIVKNQIFSHGTVASRPPVQIEELTEKPGGILVRWCKVDDDFIPQDYRLQYRKSTANHFEDVYVGSEMEFIVLHIDPNVDYQFRVCARGDGRQEWSPWSIPQTGRSTLVPHEWTAGLEGYSLSSRRNIALRNDSQSCGVLYSKAPTYFCGQTLTFRIETVGQPDKRDSIGVCVEQQNGYDSLQRDKAVCISTNGAVFVNGKEMTNQLPAVTCGSTVTFDMEVVHLGPNSNEGGNTKLRVTISSNNREVVFDWVLDHSCGSLYFGCSFSYPGWKVLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZ12</accession>
    <name>A0A452GZ12_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GZ12"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007383"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008531.1">
        <property type="protein sequence ID" value="ENSGAGP00000007383.1"/>
        <property type="gene ID" value="ENSGAGG00000005929.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042319">
        <property type="entry name" value="GINM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28549">
        <property type="entry name" value="GLYCOPROTEIN INTEGRAL MEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28549:SF1">
        <property type="entry name" value="GLYCOPROTEIN INTEGRAL MEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="325" mass="36880" checksum="EA0AB0152B9F626F" modified="2019-05-08" version="1">MAHARRGGCLLRLVAVLQDWGREGSGLPRWLELLRETRENIRVNVTMLKTSEESQEAQVVFNITYNSGQIYVNDFPMKRGVTRIKCQTLILETGNSDNLLDQRHWGIVSVRILFREWPLASRSNLQLIVIQEEVTEIDGREVQQEEVTEINILIKGLRVLRHSNHTVPLKESMLYSIPRDNDVLFTLPNLSGKVDVQGPLQPTSHYLLRQAETTVDEETLPGKLPETPLRTEPPSSYKVMCQWVEYLRKELCGFWLTSFPVFFKLMEVIGTGVVGAALVLGILKVLFPSCEHKGVLHLEDINTTAGLVINISSDIPEKTDNLVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZ80</accession>
    <name>A0A452GZ80_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">valine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Valyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452GZ80"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007584"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008754.1">
        <property type="protein sequence ID" value="ENSGAGP00000007584.1"/>
        <property type="gene ID" value="ENSGAGG00000006067.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.380:FF:000002">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="entry name" value="Valyl-tRNA synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019499">
        <property type="entry name" value="Val-tRNA_synth_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF109">
        <property type="entry name" value="VALINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10458">
        <property type="entry name" value="Val_tRNA-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="11">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="12">
        <location>
            <begin position="122"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Valyl-tRNA synthetase tRNA-binding arm" evidence="13">
        <location>
            <begin position="278"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="276"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="346" mass="37811" checksum="D8559ED413C1C32F" modified="2019-05-08" version="1">MSKSLGNVIDPLDVISGITLEGLHAQLLESNLEPAEVERARQGQKSDYPAGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFAVRSLGAEFQPPPGPEPSGAESLIDRWILSRLSLAVELSDAGFRAYDFPGVTTAIYNFWLYELCDVYLVRGALGRGGADPWFCWRDGGLEGSVEFSLGIIRAVRSLRADYSLTRTRADCFLQCLDSGAADAASRCSAYIRSLSSSGLVRVLGPGEAPPAGCAVAVASDKCTVHLLLKGLIDAEKEIAKLSGKQGELQKQIDRLRERMETPDYGTKVPPKVQESDAAKLQQSQTELQKVTEAMETFQRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H018</accession>
    <name>A0A452H018_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exocyst complex component 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Exocyst complex component Sec6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Interacts with EXOC3L1. Interacts with BIRC6/bruce. Interacts with MYRIP. Interacts with SLC6A9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SEC6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H018"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007800"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008991.1">
        <property type="protein sequence ID" value="ENSGAGP00000007800.1"/>
        <property type="gene ID" value="ENSGAGG00000006215.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000004">
        <property type="entry name" value="Exocyst complex component 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.70:FF:000001">
        <property type="entry name" value="Exocyst complex component 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF13">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="745" mass="86259" checksum="6202875CECCC5FCA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2Y3</accession>
    <name>A0A452H2Y3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">1-acyl-sn-glycerol-3-phosphate acyltransferase</fullName>
            <ecNumber evidence="19">2.3.1.51</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(11Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate = 1-(9Z)-octadecenoyl-2-(11Z)-octadecenoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37603"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:75121"/>
            <dbReference type="ChEBI" id="CHEBI:75122"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:37604"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1-(6Z,9Z,12Z-octadecatrienoyl)-sn-glycero-3-phosphate + (9Z)-octadecenoyl-CoA = (6Z,9Z,12Z)-octadecatrienoyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:74581"/>
            <dbReference type="ChEBI" id="CHEBI:74582"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:37180"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1-(9Z,12Z,15Z)-octadecatrienoyl-sn-glycero-3-phosphate + (9Z)-octadecenoyl-CoA = 1-(9Z,12Z,15Z)-octadecatrienoyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37139"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:74549"/>
            <dbReference type="ChEBI" id="CHEBI:74550"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:37140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + (9Z)-octadecenoyl-CoA = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37131"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:37132"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + (9Z,12Z)-octadecadienoyl-CoA = 1-(9Z)-octadecenoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37159"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74563"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:37160"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + hexadecanoyl-CoA = 1-(9Z)-octadecenoyl-2-hexadecanoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37143"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74551"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:37144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + tetradecanoyl-CoA = 1-(9Z)-octadecenoyl-2-tetradecanoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37171"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74579"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:37172"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1-eicosanoyl-sn-glycero-3-phosphate + (9Z)-octadecenoyl-CoA = 1-eicosanoyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37183"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:74583"/>
            <dbReference type="ChEBI" id="CHEBI:74584"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:37184"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1-hexadecanoyl-sn-glycero-3-phosphate + (9Z)-octadecenoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:33187"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:57518"/>
            <dbReference type="ChEBI" id="CHEBI:64839"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:33188"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1-tetradecanoyl-sn-glycerol 3-phosphate + (9Z)-octadecenoyl-CoA = 1-tetradecanoyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37187"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:72683"/>
            <dbReference type="ChEBI" id="CHEBI:74586"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:37188"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:19710"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>heptadecanoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate = 1-(9Z)-octadecenoyl-2-heptadecanoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37155"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:74307"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74558"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:37156"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>pentadecanoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate = 1-(9Z)-octadecenoyl-2-pentadecanoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37175"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:74309"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74578"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:37176"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.</text>
    </comment>
    <comment type="domain">
        <text evidence="19">The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 19">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A452H2Y3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008971"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010305.1">
        <property type="protein sequence ID" value="ENSGAGP00000008971.1"/>
        <property type="gene ID" value="ENSGAGG00000007090.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07989">
        <property type="entry name" value="LPLAT_AGPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004552">
        <property type="entry name" value="AGP_acyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00530">
        <property type="entry name" value="AGP_acyltrn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434:SF65">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="19" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="19" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0732">Signal</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019464416" description="1-acyl-sn-glycerol-3-phosphate acyltransferase" evidence="21">
        <location>
            <begin position="26"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="22">
        <location>
            <begin position="93"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00000816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="274" mass="30939" checksum="ECEBC45A9F853E5F" modified="2019-05-08" version="1">MELSLSQWALALLLLAVPLLYECSATFRYFCKMGFYNGWILTLAVLVIPLCALRGRSVENMKIIRFMLHHVKYLYGIRIEVRGLEHFNLPEPFVVVSNHQSSLDLLGMMEVLPDRCVPIAKKELMYMGSVGLACWLGGIIFINRKKTDDAIGVMSETAQTMLRDNVRVWVFPEGTRNHNGSMLPFKRGAFHLAVQAQVPVIPIVMSSYHDFYSKKERRFTTGRCLIRVLAPMPTRGLGPEAVPELTERVRQAMLEAFDSLSGKLSPGRDPPAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3B9</accession>
    <name>A0A452H3B9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3B9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009119"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010466.1">
        <property type="protein sequence ID" value="ENSGAGP00000009119.1"/>
        <property type="gene ID" value="ENSGAGG00000007200.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15953">
        <property type="entry name" value="7tmA_OR52P-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000006">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF132">
        <property type="entry name" value="OLFACTORY RECEPTOR 654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="43"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="312" mass="34568" checksum="8358ADBC9195D7A5" modified="2019-05-08" version="1">MASFNLTSSDPSAFTLMGIPGLEAVHILISIPFCMFYIIGLLGNFTVLFVVGKEQTLHKPMYLLLCMPALTDISTSTSVVPKALCIFWFNLKGITVGCCLSQMFFLHAASVMHSAILVTMAFDRYVAICNPLRYATILTNARIAKLGLVGLVRAVLFILPVPLLLIRQPFCANRIIPHTHCDHMAVAKMSCGDITANRIYSLITTLVVIGFDLMLIALSYSLIIRAVLRISSKKAHQKALNTCTAHICVMLTYYTPGLFSNLTYWFGQGIAPHVHIILADLYLLIPPMLNPIIYGVKTKEFRDKVGIYTCKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4T1</accession>
    <name>A0A452H4T1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">A kinase-anchoring proteins AKAP-5 and AKAP-12 calmodulin (CaM)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000009497"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010912.1">
        <property type="protein sequence ID" value="ENSGAGP00000009497.1"/>
        <property type="gene ID" value="ENSGAGG00000007516.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051018">
        <property type="term" value="F:protein kinase A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010739">
        <property type="term" value="P:positive regulation of protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090036">
        <property type="term" value="P:regulation of protein kinase C signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028540">
        <property type="entry name" value="AKAP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001573">
        <property type="entry name" value="AKAP_WSK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23209">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23209:SF4">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03832">
        <property type="entry name" value="WSK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51893">
        <property type="entry name" value="AKAP_CAM_BD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="A kinase-anchoring proteins AKAP-5 and AKAP-12 calmodulin (CaM)-binding" evidence="7">
        <location>
            <begin position="653"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="A kinase-anchoring proteins AKAP-5 and AKAP-12 calmodulin (CaM)-binding" evidence="7">
        <location>
            <begin position="805"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="domain" description="A kinase-anchoring proteins AKAP-5 and AKAP-12 calmodulin (CaM)-binding" evidence="7">
        <location>
            <begin position="850"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="100"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="186"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="422"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="462"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="523"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1350"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1701"/>
            <end position="1723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1902"/>
            <end position="1930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2077"/>
            <end position="2169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="213"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="292"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="353"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="484"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="562"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="611"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="671"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="687"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="701"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="780"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="841"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1902"/>
            <end position="1920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2078"/>
            <end position="2088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2095"/>
            <end position="2112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2128"/>
            <end position="2153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2158"/>
            <end position="2169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2169" mass="238308" checksum="D7A8000B13996AF5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6E8</accession>
    <name>A0A452H6E8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">WD repeat-containing protein 90</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Microtubule-binding protein that plays a crucial role in ensuring inner core protein localization within the centriole core, as well as in maintaining the microtubule wall integrity and the overall centriole roundness and stability. Required for efficient primary cilium formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriolar satellite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the WD repeat WDR90/POC16 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000010252"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011775.1">
        <property type="protein sequence ID" value="ENSGAGP00000010252.1"/>
        <property type="gene ID" value="ENSGAGG00000008033.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000522">
        <property type="entry name" value="WD repeat domain 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000590">
        <property type="entry name" value="WD repeat domain 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000772">
        <property type="entry name" value="WD repeat domain 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001417">
        <property type="entry name" value="WD repeat domain 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055439">
        <property type="entry name" value="Beta-prop_EML_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055440">
        <property type="entry name" value="Beta-prop_WDR90_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055441">
        <property type="entry name" value="Beta-prop_WDR90_POC16_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007714">
        <property type="entry name" value="CFA20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF24">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23409">
        <property type="entry name" value="Beta-prop_EML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23393">
        <property type="entry name" value="Beta-prop_WDR90_POC16_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05018">
        <property type="entry name" value="CFA20_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23342">
        <property type="entry name" value="WDR90_beta-prop_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="663"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="747"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="1467"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="15">
        <location>
            <begin position="1708"/>
            <end position="1781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1010"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1011"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1092"/>
            <end position="1112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1786" mass="196815" checksum="3672443D9AE917DD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7L9</accession>
    <name>A0A452H7L9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H7L9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010709"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012279.1">
        <property type="protein sequence ID" value="ENSGAGP00000010709.1"/>
        <property type="gene ID" value="ENSGAGG00000008353.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030169">
        <property type="term" value="F:low-density lipoprotein particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016322">
        <property type="term" value="P:neuron remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000013">
        <property type="entry name" value="Scavenger receptor class F, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000041">
        <property type="entry name" value="Tyrosine protein kinase receptor tie-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042635">
        <property type="entry name" value="MEGF10/SREC1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24043">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24043:SF0">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019003152" description="EGF-like domain-containing protein" evidence="15">
        <location>
            <begin position="22"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="50"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="586"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="758"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="606"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="758"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="778"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="810"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="852"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="901" mass="96996" checksum="014B405D9855AF9B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9I2</accession>
    <name>A0A452H9I2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H9I2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011472"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013133.1">
        <property type="protein sequence ID" value="ENSGAGP00000011472.1"/>
        <property type="gene ID" value="ENSGAGG00000008851.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22796:SF6">
        <property type="entry name" value="INTERFERON-INDUCED VERY LARGE GTPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22796">
        <property type="entry name" value="URG4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="334" mass="38317" checksum="E989FF164954AB10" modified="2019-05-08" version="1">ISFKIACQGATSITTFADILCSNLKSAICHAIYDKTAIDIANEMRSKHPAFSGNRSRLELFMLVCLLKQEDFEKYQQYIYNPSYYMEDFIRREVDKYCLDKKNPKLKNFLNLNLDSFHSLVLLAIHESIKVVKDKHGNVASWLDEFCTRLGDDMYLPRSNLTSIEHQETKDIQFLEQVVSESLIPALEELKQTFSEIDLDPFQCSFCKAVCTNTIPGHDGDHSTHFHRPQALAGIQWEGTNHLVTDICTTLAASDCKIVLDGKTIPCKNYREVGSDYANWDIKPVTSQLSYWKWFVSHFRSDLEKIHNGKFKGKGAIPTNWNNLNKDKVLDWKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAC8</accession>
    <name>A0A452HAC8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DIS3-like exonuclease 2</fullName>
            <ecNumber evidence="12">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dis3l2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of both mRNAs and miRNAs that have been polyuridylated by a terminal uridylyltransferase, such as ZCCHC11/TUT4. Mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'. Mediates degradation of uridylated pre-let-7 miRNAs, contributing to the maintenance of embryonic stem (ES) cells. Essential for correct mitosis, and negatively regulates cell proliferation.</text>
    </comment>
    <comment type="function">
        <text evidence="12">3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of both mRNAs and miRNAs that have been polyuridylated by a terminal uridylyltransferase. Essential for correct mitosis, and negatively regulates cell proliferation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
            <location evidence="12">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">Specifically recognizes and binds polyuridylated RNAs via 3 RNA-binding regions (named U-zone 1, U-zone 2 and U-zone 3) that form an open funnel on one face of the catalytic domain, allowing RNA to navigate a path to the active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the RNR ribonuclease family. DIS3L2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452HAC8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011682"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013384.1">
        <property type="protein sequence ID" value="ENSGAGP00000011682.1"/>
        <property type="gene ID" value="ENSGAGG00000009026.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010587">
        <property type="term" value="P:miRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990074">
        <property type="term" value="P:polyuridylation-dependent mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000177">
        <property type="entry name" value="DIS3-like exonuclease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.690:FF:000003">
        <property type="entry name" value="DIS3-like exonuclease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000003">
        <property type="entry name" value="DIS3-like exonuclease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03045">
        <property type="entry name" value="DIS3L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028591">
        <property type="entry name" value="DIS3L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041093">
        <property type="entry name" value="Dis3l2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033771">
        <property type="entry name" value="Rrp44_CSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF9">
        <property type="entry name" value="DIS3-LIKE EXONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17877">
        <property type="entry name" value="Dis3l2_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17216">
        <property type="entry name" value="Rrp44_CSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 12" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0498">Mitosis</keyword>
    <keyword evidence="3 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNB" evidence="14">
        <location>
            <begin position="635"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="164"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="218"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="387"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="240"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="387"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="647"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="656"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="12">
        <location>
            <position position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1128" mass="127289" checksum="37866699242ABB36" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBI0</accession>
    <name>A0A452HBI0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Envoplakin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">210 kDa cornified envelope precursor protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">p210</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">May form a homodimer or a heterodimer with PPL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cornified envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the plakin or cytolinker family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000012120"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013881.1">
        <property type="protein sequence ID" value="ENSGAGP00000012120.1"/>
        <property type="gene ID" value="ENSGAGG00000009326.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001533">
        <property type="term" value="C:cornified envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000178">
        <property type="entry name" value="Envoplakin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000010">
        <property type="entry name" value="Envoplakin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.780:FF:000002">
        <property type="entry name" value="Envoplakin b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000142">
        <property type="entry name" value="Envoplakin like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000109">
        <property type="entry name" value="Periplakin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000088">
        <property type="entry name" value="Periplakin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000030">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1290.10">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041615">
        <property type="entry name" value="Desmoplakin_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035915">
        <property type="entry name" value="Plakin_repeat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058847">
        <property type="entry name" value="Plectin_PPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001101">
        <property type="entry name" value="Plectin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055419">
        <property type="entry name" value="Spectrin_PEPL/EVPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169:SF7">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00681">
        <property type="entry name" value="Plectin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26346">
        <property type="entry name" value="Plectin_PPL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17902">
        <property type="entry name" value="SH3_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23160">
        <property type="entry name" value="Spectrin_1st_PEPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21097">
        <property type="entry name" value="SR_plectin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00250">
        <property type="entry name" value="PLEC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75399">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0417">Keratinization</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Periplakin/Envoplakin N-terminal" evidence="21">
        <location>
            <begin position="33"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Desmoplakin SH3" evidence="20">
        <location>
            <begin position="390"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Periplakin-like plectin repeat" evidence="22">
        <location>
            <begin position="955"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="domain" description="Periplakin-like plectin repeat" evidence="22">
        <location>
            <begin position="1119"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="domain" description="Periplakin-like plectin repeat" evidence="22">
        <location>
            <begin position="1193"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="domain" description="Periplakin-like plectin repeat" evidence="22">
        <location>
            <begin position="1414"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="480"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="321"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="873"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="961"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="1199"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="1377"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="1467"/>
            <end position="1501"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="1532"/>
            <end position="1604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF17902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF23160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF26346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2028" mass="234566" checksum="F6A619D7CA133CEC" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCF4</accession>
    <name>A0A452HCF4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Promyelocytic leukemia</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HCF4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012449"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014248.1">
        <property type="protein sequence ID" value="ENSGAGP00000012449.1"/>
        <property type="gene ID" value="ENSGAGG00000009550.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044790">
        <property type="term" value="P:suppression of viral release by host"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19804">
        <property type="entry name" value="Bbox1_TRIM19_C-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19770">
        <property type="entry name" value="Bbox2_TRIM19_C-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16579">
        <property type="entry name" value="RING-HC_PML_C-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021978">
        <property type="entry name" value="PML-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF302">
        <property type="entry name" value="PROTEIN PML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22586">
        <property type="entry name" value="ANCHR-like_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12126">
        <property type="entry name" value="PML_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="45"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="115"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="178"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="226"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="568" mass="63139" checksum="D76FABD5A5162FEB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HD91</accession>
    <name>A0A452HD91_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase assembly factor 3</fullName>
            <shortName evidence="6">SDH assembly factor 3</shortName>
            <shortName evidence="6">SDHAF3</shortName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the iron-sulfur protein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the complex I LYR family. SDHAF3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HD91"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012768"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014635.1">
        <property type="protein sequence ID" value="ENSGAGP00000012768.1"/>
        <property type="gene ID" value="ENSGAGG00000009798.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006105">
        <property type="term" value="P:succinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20270">
        <property type="entry name" value="Complex1_LYR_SDHAF3_LYRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008381">
        <property type="entry name" value="SDHAF3/Sdh7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137">
        <property type="entry name" value="DC11 ACN9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137:SF6">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="75" mass="8757" checksum="E337DCE86DD63CDD" modified="2019-05-08" version="1">MPSPAALHVSRVRALYRRILLLHRALPVALKALGDEYVKEEFRRHRSVGPADAQRFLREWEASLDKCNLFIIILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEC4</accession>
    <name>A0A452HEC4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">b(0,+)-type amino acid transporter 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Glycoprotein-associated amino acid transporter b0,+AT1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Solute carrier family 7 member 9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-phenylalanine(out) + L-arginine(in) = L-phenylalanine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71067"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:71068"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-cysteine(out) + L-arginine(in) = L-cysteine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71071"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:71072"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cystine(out) + L-arginine(in) = L-cystine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71075"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:35491"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:71076"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-histidine(out) + L-arginine(in) = L-histidine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71063"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:71064"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-leucine(out) + L-arginine(in) = L-leucine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71059"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:71060"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysine(out) + L-arginine(in) = L-lysine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:70827"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:70828"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HEC4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013152"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015057.1">
        <property type="protein sequence ID" value="ENSGAGP00000013152.1"/>
        <property type="gene ID" value="ENSGAGG00000010081.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000015">
        <property type="entry name" value="B(0,+)-type amino acid transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF354">
        <property type="entry name" value="B(0,+)-TYPE AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="309"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00083296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00093193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="493" mass="54078" checksum="78DD78830E8B99CB" modified="2019-05-08" version="1">MGEENMRKRKGETNRESDEQSIQSKESKTMNLQKEVGLISGICIIVGTVIGSGIFISPKSVLANVGAVGPCLVIWAACGVLSTLGALCFAELGTMITKSGGEYPYLMEAFGPIPAYLFSWTSLLVTKPSSFAIICLSFAEYASAPFYPGCDPPVVVIKCLAAAAIVIITTVNSLSVKLGSYVQNVFTAAKMIIVMIIIVSGIVLLAKGKTENFKNSFNDAKISAGSISLAFYNGLWAYDGWNQLNYITEELKNPYRNLPLSIIIGIPLVSVCYVLINISYFTIMTSTELLQSQAVAVTFGDRVLYPASWIVPLFVAFSTIGAANGTCFTAGRLVYVAGREGHMLKVLSYISVKRFTPAPAIIFYGAIAIIYIIPGDINTLINYFSFAVWIFYALTVFALIVMRFTRKELKRPIKIPIIIPVLVTIVSILLVLAPIISEPEWAYLYCVLFILGGFIFYVLFIHFKFNWAQKISGPITMYLQMLLEVVPPEKVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HF43</accession>
    <name>A0A452HF43_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DENND6 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000013422"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015358.1">
        <property type="protein sequence ID" value="ENSGAGP00000013422.1"/>
        <property type="gene ID" value="ENSGAGG00000010139.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018307">
        <property type="entry name" value="ABL9/DENND6_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024224">
        <property type="entry name" value="DENND6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13677">
        <property type="entry name" value="LD41638P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13677:SF1">
        <property type="entry name" value="PROTEIN DENND6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09794">
        <property type="entry name" value="Avl9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="3">
        <location>
            <begin position="122"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="61"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="667" mass="76764" checksum="6902CECB06709B08" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJ79</accession>
    <name>A0A452HJ79_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HJ79"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014930"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017055.1">
        <property type="protein sequence ID" value="ENSGAGP00000014930.1"/>
        <property type="gene ID" value="ENSGAGG00000011281.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01272">
        <property type="entry name" value="PTB1_Fe65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01271">
        <property type="entry name" value="PTB2_Fe65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000019">
        <property type="entry name" value="Amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000003">
        <property type="entry name" value="amyloid beta A4 precursor protein-binding family B member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000034">
        <property type="entry name" value="amyloid beta A4 precursor protein-binding family B member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039576">
        <property type="entry name" value="APBB1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14058">
        <property type="entry name" value="AMYLOID BETA A4 PRECURSOR PROTEIN-BINDING FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14058:SF11">
        <property type="entry name" value="AMYLOID BETA PRECURSOR PROTEIN BINDING FAMILY B MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WW" evidence="5">
        <location>
            <begin position="299"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="4">
        <location>
            <begin position="407"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="4">
        <location>
            <begin position="577"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="219"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="335"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="269"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="335"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="746" mass="82397" checksum="C9BC5EF66AF1E0E6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKD9</accession>
    <name>A0A452HKD9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HKD9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015387"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017574.1">
        <property type="protein sequence ID" value="ENSGAGP00000015387.1"/>
        <property type="gene ID" value="ENSGAGG00000011608.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000204">
        <property type="entry name" value="Major histocompatibility complex, class I-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF67">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="183"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="322"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="339" mass="36885" checksum="0463C51050555C17" modified="2019-05-08" version="1">MSSCPGPVVQHSWDILFTGDQGSKGISNFFVLGQVDGLGVAYYDSTTLKLSPTWDWMCRANRACSADPQTLLHPQSPCDAASFSVPQQGCTRCSCGWAAPCGMTSLQAGVLQYAYDSQDFIGFDGQRGLWVAAVQPSVLSKQHWEADRPWCQFLQHYLQQECVETLRSLVQHGRETLGCELPPEVSVSCRDSPDGSVTISCHARGFYPPPIHVSWVRDEEDILAETDSSGILPNADSTYYTQSSLEISLQQDWHPYACRVEHTSLPEPTLIWVPSKKGPLSPGVLVSLVLAMLAGAGAAVIGVIMWRRNHSGKGMGEDLWGQGWGGRAQGGGRVGGQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKY8</accession>
    <name>A0A452HKY8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the BET family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000015593"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017810.1">
        <property type="protein sequence ID" value="ENSGAGP00000015593.1"/>
        <property type="gene ID" value="ENSGAGG00000011754.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05497">
        <property type="entry name" value="Bromo_Brdt_I_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05498">
        <property type="entry name" value="Bromo_Brdt_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000003">
        <property type="entry name" value="Bromodomain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.220:FF:000001">
        <property type="entry name" value="bromodomain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000002">
        <property type="entry name" value="Bromodomain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031354">
        <property type="entry name" value="BRD4_CDT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043508">
        <property type="entry name" value="Bromo_Brdt_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043509">
        <property type="entry name" value="Bromo_Brdt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050935">
        <property type="entry name" value="Bromo_chromatin_reader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880:SF143">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880">
        <property type="entry name" value="FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17105">
        <property type="entry name" value="BRD4_CDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="11">
        <location>
            <begin position="75"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="11">
        <location>
            <begin position="366"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="12">
        <location>
            <begin position="600"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="176"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="459"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="673"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="924"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="214"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="317"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="479"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="533"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="552"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="699"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="724"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="752"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="854"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="873"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="928"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="960"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1123"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1159"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1171"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1236"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1268"/>
            <end position="1284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1307" mass="147332" checksum="3781171422B55CE4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HPI8</accession>
    <name>A0A452HPI8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">U1 small nuclear ribonucleoprotein 70 kDa</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HPI8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016921"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019313.1">
        <property type="protein sequence ID" value="ENSGAGP00000016921.1"/>
        <property type="gene ID" value="ENSGAGG00000012628.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000514">
        <property type="entry name" value="Small nuclear ribonucleoprotein 70 (U1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022023">
        <property type="entry name" value="U1snRNP70_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF5">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12220">
        <property type="entry name" value="U1snRNP70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="139"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="196" mass="23207" checksum="44E0A7318D8C6054" modified="2019-05-08" version="1">MTQFLPPNLLALFAPRDPIPYLPPLEKLPHEKHHNQPYCGIAPYIREFEDPRDAPPPTRAETREERMERKVRLSAKPPLKSCPGVPLWRVTARRPEPVTPSLSFPQRREKIERRQQEVENELKMWDPHNDPNAQGDAFKTLFVARVNYDTTESKLRREFEVYGPIKRIYMVYSKRSGKPRGYAFIEYEHERDMHCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQ34</accession>
    <name>A0A452HQ34_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQ34"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017135"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019556.1">
        <property type="protein sequence ID" value="ENSGAGP00000017135.1"/>
        <property type="gene ID" value="ENSGAGG00000012797.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071787">
        <property type="term" value="P:endoplasmic reticulum tubular network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2480:FF:000001">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046964">
        <property type="entry name" value="RTN1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799:SF1">
        <property type="entry name" value="RETICULON-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="841"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="950"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="8">
        <location>
            <begin position="821"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU210713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1008" mass="111211" checksum="12F897ADAC90693A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSP8</accession>
    <name>A0A452HSP8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphodiesterase</fullName>
            <ecNumber evidence="7">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="7">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452HSP8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018044"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020579.1">
        <property type="protein sequence ID" value="ENSGAGP00000018044.1"/>
        <property type="gene ID" value="ENSGAGG00000013035.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000005">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000007">
        <property type="entry name" value="Phosphodiesterase 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0140">cGMP</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="9">
        <location>
            <begin position="192"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="510"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="265"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="534" mass="61185" checksum="797D44DBD190C3F5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSQ4</accession>
    <name>A0A452HSQ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cyclin-dependent kinase-like 3</fullName>
            <ecNumber evidence="3">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452HSQ4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020619.1">
        <property type="protein sequence ID" value="ENSGAGP00000018082.1"/>
        <property type="gene ID" value="ENSGAGG00000013399.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097484">
        <property type="term" value="P:dendrite extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030517">
        <property type="term" value="P:negative regulation of axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050775">
        <property type="term" value="P:positive regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000049">
        <property type="entry name" value="cyclin-dependent kinase-like 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000370">
        <property type="entry name" value="cyclin-dependent kinase-like 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF177">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="4"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="359"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="439"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="552"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="366"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="386"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="577" mass="65395" checksum="15346B5CD4200030" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HU39</accession>
    <name>A0A452HU39_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat and fibronectin type-III domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Cell adhesion molecule that mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Promotes neurite outgrowth in hippocampal neurons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the LRFN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HU39"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018620"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021228.1">
        <property type="protein sequence ID" value="ENSGAGP00000018620.1"/>
        <property type="gene ID" value="ENSGAGG00000013776.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098982">
        <property type="term" value="C:GABA-ergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905606">
        <property type="term" value="P:regulation of presynapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099560">
        <property type="term" value="P:synaptic membrane adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05764">
        <property type="entry name" value="IgI_SALM5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000025">
        <property type="entry name" value="Leucine rich repeat and fibronectin type III domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000235">
        <property type="entry name" value="Leucine-rich repeat and fibronectin type III domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000091">
        <property type="entry name" value="Leucine-rich repeat and fibronectin type III domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000016">
        <property type="entry name" value="Leucine-rich repeat and fibronectin type III domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF10">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 16" id="KW-0732">Signal</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018969148" description="Leucine-rich repeat and fibronectin type-III domain-containing protein 5" evidence="16">
        <location>
            <begin position="18"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="525"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="287"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="385"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="582"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="601"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="612"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="660"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="776" mass="85573" checksum="9EFAB80539963C2D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUH3</accession>
    <name>A0A452HUH3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prefoldin subunit 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Component of the PAQosome complex which is responsible for the biogenesis of several protein complexes and which consists of R2TP complex members RUVBL1, RUVBL2, RPAP3 and PIH1D1, URI complex members PFDN2, PFDN6, PDRG1, UXT and URI1 as well as ASDURF, POLR2E and DNAAF10/WDR92.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HUH3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018799"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021430.1">
        <property type="protein sequence ID" value="ENSGAGP00000018799.1"/>
        <property type="gene ID" value="ENSGAGG00000013911.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23161">
        <property type="entry name" value="Prefoldin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000003">
        <property type="entry name" value="Prefoldin subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018154">
        <property type="entry name" value="TLV/ENV_coat_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00429">
        <property type="entry name" value="TLV_coat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58069">
        <property type="entry name" value="Virus ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019483629" description="Prefoldin subunit 6" evidence="7">
        <location>
            <begin position="22"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="138"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="216"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00064783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="262" mass="29661" checksum="7330E34912BA2D0A" modified="2019-05-08" version="1">MVLQNRMALDILLASQGGTCALIGQECCVYIPDVYNTTWQRAKHLEKIARDHGGKRPSWNSWWSDFFSWLPEPRRAQLALIRQGEEGDGLRGRTTNPMVHRGARQTWTVRKPGGEVACAARRGAQHRSSGGPDRTLMADLIQKKLQGELEKYQQLQKDLSKSMAARQKLEAQLTENNIVKEELGLLDATNTVYKLIGPVLVKQDMDEAKATVSKRLDYIAGEIKRYELQMQESEKKAEQQREVLARLQQDYQRSQGKGAAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUI0</accession>
    <name>A0A452HUI0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WD repeat-containing protein 46</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Scaffold component of the nucleolar structure. Required for localization of DDX21 and NCL to the granular compartment of the nucleolus. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3. Interacts with DDX21, NCL, NOP2 and EBNA1BP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HUI0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018804"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021436.1">
        <property type="protein sequence ID" value="ENSGAGP00000018804.1"/>
        <property type="gene ID" value="ENSGAGG00000013915.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000128">
        <property type="entry name" value="WD repeat domain 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012952">
        <property type="entry name" value="BING4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040315">
        <property type="entry name" value="WDR46/Utp7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085">
        <property type="entry name" value="WD-REPEAT PROTEIN BING4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08149">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01033">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="343"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="BING4 C-terminal" evidence="12">
        <location>
            <begin position="424"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="527"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="556"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="595" mass="65929" checksum="993BA896D9B9DBCF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HV04</accession>
    <name>A0A452HV04_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HV04"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018950"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021606.1">
        <property type="protein sequence ID" value="ENSGAGP00000018950.1"/>
        <property type="gene ID" value="ENSGAGG00000014026.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13261">
        <property type="entry name" value="PH_RasGRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.870.10:FF:000004">
        <property type="entry name" value="Ras-specific guanine nucleotide-releasing factor 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000005">
        <property type="entry name" value="Ras-specific guanine nucleotide-releasing factor 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.870.10:FF:000006">
        <property type="entry name" value="ras-specific guanine nucleotide-releasing factor 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000117">
        <property type="entry name" value="ras-specific guanine nucleotide-releasing factor 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000176">
        <property type="entry name" value="ras-specific guanine nucleotide-releasing factor 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000004">
        <property type="entry name" value="ras-specific guanine nucleotide-releasing factor 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF187">
        <property type="entry name" value="RAS-SPECIFIC GUANINE NUCLEOTIDE-RELEASING FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="22"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="8">
        <location>
            <begin position="242"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="469"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="9">
        <location>
            <begin position="634"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="7">
        <location>
            <begin position="1005"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="710"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="762"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="839"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="762"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="793"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1240" mass="141606" checksum="FFE9E8C0593A40DD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HV89</accession>
    <name>A0A452HV89_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">LRRNT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Postsynaptic cell membrane</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the LRRTM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HV89"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019059"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021722.1">
        <property type="protein sequence ID" value="ENSGAGP00000019059.1"/>
        <property type="gene ID" value="ENSGAGG00000014098.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000005">
        <property type="entry name" value="leucine-rich repeat transmembrane neuronal protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF19">
        <property type="entry name" value="LEUCINE-RICH REPEAT TRANSMEMBRANE NEURONAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="14">
        <location>
            <begin position="15"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="360"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="495" mass="56678" checksum="D5AA694EF43EC217" modified="2019-05-08" version="1">APTVLLTMLSSAERGCPKSCRCEGKMVYCESQKLQEIPLTISAGCLGLSLRYNSLQKLKHNQFKGLNQLTWLYLDHNHISNIDENAFNGIRRLKELILSSNRIAYFLNNTFRPVTNLRNLDLSYNQLQSLGSEQFRGLRKLLSLHLRSNSLRTIPVRIFQDCRNLELLDLGYNRIRSLARNVFAGMIRLKELHLEHNQFSKLNLALFPRLVSLQNLYLQWNKISVIGQTMSWTWSSLQRLDLSGNEIEAFSGPSVFQCVPNLQRLNLDSNKLTFIGQEILDSWISLNDISLAGNIWECSRNICSLVNWLKSFKGLRENTIICASPKELQGVNVIDAVKNYSICGKSTTERFELARAPTKPTFKPKLTRPKHDSKPPLPPTIETTESSSELEHDTEHISFHKIIAGSVALFLSVLVILLVIYVSWKRYPASMKQLQQHSLMRRHRKKKRQSLKQMTPTTQEFYVDYKPTNTETSEMLLNGTGPCTYNKSGSRECEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVN0</accession>
    <name>A0A452HVN0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histidine kinase/HSP90-like ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HVN0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019139"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021809.1">
        <property type="protein sequence ID" value="ENSGAGP00000019139.1"/>
        <property type="gene ID" value="ENSGAGG00000014151.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000204">
        <property type="entry name" value="Heat shock protein HSP 90-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="10">
        <location>
            <begin position="35"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="222"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="701"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="108"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="128"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="726" mass="83220" checksum="B56D8849A78D6E32" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWW9</accession>
    <name>A0A452HWW9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="25">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18373"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57658"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:18374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000019640"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022371.1">
        <property type="protein sequence ID" value="ENSGAGP00000019640.1"/>
        <property type="gene ID" value="ENSGAGG00000014491.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035005">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04012">
        <property type="entry name" value="C2A_PI3K_class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08381">
        <property type="entry name" value="C2B_PI3K_class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00869">
        <property type="entry name" value="PI3Ka_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00895">
        <property type="entry name" value="PI3Kc_C2_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07290">
        <property type="entry name" value="PX_PI3K_C2_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000006">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000003">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000005">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000036">
        <property type="entry name" value="phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000065">
        <property type="entry name" value="phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000105">
        <property type="entry name" value="phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000001">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000341">
        <property type="entry name" value="PI3K_Ras-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042134">
        <property type="entry name" value="PX_PI3K_C2_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF30">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-PHOSPHATE 3-KINASE C2 DOMAIN-CONTAINING SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00794">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00144">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51546">
        <property type="entry name" value="PI3K_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K-RBD" evidence="23">
        <location>
            <begin position="375"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="C2 PI3K-type" evidence="24">
        <location>
            <begin position="627"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="22">
        <location>
            <begin position="805"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="21">
        <location>
            <begin position="1050"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="20">
        <location>
            <begin position="1365"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="19">
        <location>
            <begin position="1504"/>
            <end position="1624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="92"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1634" mass="185615" checksum="DE0FFC5A939A1022" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HX97</accession>
    <name>A0A452HX97_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Phosphatidylinositol 3-kinase catalytic subunit type 3</fullName>
            <ecNumber evidence="4 16">2.7.1.137</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="4 16"/>
    <dbReference type="AlphaFoldDB" id="A0A452HX97"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019767"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022522.1">
        <property type="protein sequence ID" value="ENSGAGP00000019767.1"/>
        <property type="gene ID" value="ENSGAGG00000014578.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08397">
        <property type="entry name" value="C2_PI3K_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00870">
        <property type="entry name" value="PI3Ka_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00896">
        <property type="entry name" value="PI3Kc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000003">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000043">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057756">
        <property type="entry name" value="PI3-kinase_type3/VPS34_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008290">
        <property type="entry name" value="PI3K_Vps34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE CATALYTIC SUBUNIT TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000587">
        <property type="entry name" value="PI3K_Vps34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2 PI3K-type" evidence="20">
        <location>
            <begin position="35"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="19">
        <location>
            <begin position="292"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="18">
        <location>
            <begin position="614"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="149"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="432"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="870" mass="99518" checksum="B20AE070846A2795" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXS5</accession>
    <name>A0A452HXS5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HXS5"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022817.1">
        <property type="protein sequence ID" value="ENSGAGP00000020029.1"/>
        <property type="gene ID" value="ENSGAGG00000014760.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039089">
        <property type="entry name" value="IGSF6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15297">
        <property type="entry name" value="IMMUNOGLOBULIN SUPERFAMILY MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15297:SF2">
        <property type="entry name" value="IMMUNOGLOBULIN SUPERFAMILY MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019386952" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="46"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="234" mass="26630" checksum="1B2BF591E6409CF6" modified="2019-05-08" version="1">MAPFNKCKVVLVLEFNWILYCVGHAVDTCKVTISQHPFKEVDHSVNTVNITCSFSVSGCKGSPQMLWFRYRDFTHEALCTPECIEKFKVIGSLSDNSALLQINKLTVNDSAIYICGIAFSNSDSHTSKQTGGGTILVKRGSEKYSTEEYIPMIVLSSLLFLYSTTIFAIFIFYKSKSKLVKKTRKEDEKGEHHKNMSGQTVCRAIVQELYKKRYAQDHHQPECLEPDDTIYQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXW1</accession>
    <name>A0A452HXW1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HXW1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020064"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022855.1">
        <property type="protein sequence ID" value="ENSGAGP00000020064.1"/>
        <property type="gene ID" value="ENSGAGG00000014787.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001586">
        <property type="term" value="F:Gi/o-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051378">
        <property type="term" value="F:serotonin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099589">
        <property type="term" value="F:serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007198">
        <property type="term" value="P:adenylate cyclase-inhibiting serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15335">
        <property type="entry name" value="7tmA_5-HT1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000085">
        <property type="entry name" value="5-hydroxytryptamine receptor 1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF191">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00515">
        <property type="entry name" value="5HT1FRECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="179"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="39"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="366" mass="41538" checksum="4298A620325332D6" modified="2019-05-08" version="1">MNFTNCTTEANVAVKPKTVTEKMLITLTLAIITILTMLLNSAVITAIGTTKKLHQPANYLICSLAVTDLLVALLVMPLSITYIVMDTWTLGYFICEIWLSVDMTCCTCSILHLCVIALDRYWAITDAIEYARKRTAKRAGLMIVTVWTISIFISMPPLFWRNHHSVTIPSECRIQHDHVIYTIYSTFGAFYIPLTLILILYYRIYHAAKSLYQKRGSSRHLSNKSTDSQNSFASCNCKLTQTFCVSDFSTSDPITEFDKIHASVRFPPVDNDLELAGDRQQISSTRERKAARILGLILGAFILSWLPFFIKELIVGLSVRKVSAEVADFLTWLGYVNSLINPLLYTSFNEDFKMAFKKLIRCWENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYN6</accession>
    <name>A0A452HYN6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannosidase endo-alpha like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 99 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYN6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020296"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023121.1">
        <property type="protein sequence ID" value="ENSGAGP00000020296.1"/>
        <property type="gene ID" value="ENSGAGG00000014966.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026071">
        <property type="entry name" value="Glyco_Hydrolase_99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572">
        <property type="entry name" value="ENDO-ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572:SF2">
        <property type="entry name" value="GLYCOPROTEIN ENDO-ALPHA-1,2-MANNOSIDASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16317">
        <property type="entry name" value="Glyco_hydro_99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18331" checksum="13C03E75565346ED" modified="2019-05-08" version="1">MARLRRRACIALFLFTLFIFGTMMGLRTLKPSDGFSDLAPIAERGERRSVSHEAISRPQAATSSDAKAPTPPVYYDLHIFYYMWYGNTHFEGKYLHWDHVMVPHWDPKISASYPKGRHSPPEDIGSSFYPELGPYSSRDPEVLEEHMNQLRAAAIGKDALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYT5</accession>
    <name>A0A452HYT5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Phosphatidylinositol-4,5-bisphosphate 4-phosphatase</fullName>
            <ecNumber evidence="4 11">3.1.3.78</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) to phosphatidylinositol-4-phosphate (PtdIns-4-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25674"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57795"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="EC" id="3.1.3.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Late endosome membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 11">Lysosome membrane</location>
            <topology evidence="3 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.78" evidence="4 11"/>
    <dbReference type="AlphaFoldDB" id="A0A452HYT5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020341"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023177.1">
        <property type="protein sequence ID" value="ENSGAGP00000020341.1"/>
        <property type="gene ID" value="ENSGAGG00000014996.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034597">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019178">
        <property type="entry name" value="PtdIns-P2-Ptase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21014">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE 4-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21014:SF2">
        <property type="entry name" value="TYPE 1 PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE 4-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09788">
        <property type="entry name" value="Tmemb_55A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0967">Endosome</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="207"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="272" mass="29308" checksum="D1303CE2DEEED172" modified="2019-05-08" version="1">MGGCSLWFPCPGFPLFPDGHPAVLPGEDPPPYSPMASPDSGSAPMITCRVCQSLINVEGKMHQHVVKCSVCNEATPIKNAPPGKKYVRCPCNCLLICKVTSQRIACPRPYCKRIINLGPVHPGPLSPEPQPVGVRVICGHCKNTFLLLHRAQVPLEEMHLLPSPGCPSGCSCHRPSVWHMEARPAVQWDLRLLGAYHSPGRAVSGPGHVLGLHASQPPRPELLLSRPCRGEPRPSQSAPAQPPGPLPCLSPWSCSPPNLPEPEQETPTGRDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZE8</accession>
    <name>A0A452HZE8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HZE8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023416.1">
        <property type="protein sequence ID" value="ENSGAGP00000020559.1"/>
        <property type="gene ID" value="ENSGAGG00000015136.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="57" mass="6844" checksum="4E5B989B702ABEE0" modified="2019-05-08" version="1">MKVICLIEISSCLVKHTEVTQKYISELTELKKSKDYMQPDKTSFCIFYLFLDFDEYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZH3</accession>
    <name>A0A452HZH3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RUS1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZH3"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023497.1">
        <property type="protein sequence ID" value="ENSGAGP00000020624.1"/>
        <property type="gene ID" value="ENSGAGG00000015194.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006968">
        <property type="entry name" value="RUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055412">
        <property type="entry name" value="UVB_sens_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054549">
        <property type="entry name" value="UVB_sens_RUS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770:SF31">
        <property type="entry name" value="RUS FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770">
        <property type="entry name" value="RUS1 FAMILY PROTEIN C16ORF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24160">
        <property type="entry name" value="UVB_sens_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04884">
        <property type="entry name" value="UVB_sens_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein root UVB sensitive/RUS" evidence="6">
        <location>
            <begin position="43"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Root UVB sensitive protein C-terminal" evidence="7">
        <location>
            <begin position="281"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="448" mass="49160" checksum="936833A1C56F5610" modified="2019-05-08" version="1">MPSLGVQPLCTEQYGSRGARCYLPSPDGRLSYVGDEPGHVGWRSLKEIFMSVFLPQGYPESVSQDYLPYQIWDTVQAFASSITGALATQAVLKGVGVGDQTSTVAAATVTWILKDGTGMLGRILFAWMKGSKLDCDAKQWRLFADGLNDLAIFMEILAPAFPACFTLIICTSGFFKCIVGVAGGATRAALTMHQARRDNMADVSAKDGSQETLVNLAGLLVSLLLIPLVTDRLQLTYTLYGLFTALHLYANYRAVRAVCMETLNRARLRLALRHFLCQGWVPTPAHANPQEPLLPEFQHHLRITLGAPLHLLVSSVAEFQQACEGNLRNYLIALNRKAGTVAVALRHGASSVDIIQACSHALLLETLLCQALPPGAVEEETLQKLQWQLCEAPDGEVWAALTETHQLLDRLFPRFLAGLVSTGWVTDRNLLGAEEWRLDWTLKDKKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZY3</accession>
    <name>A0A452HZY3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZY3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020739"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023623.1">
        <property type="protein sequence ID" value="ENSGAGP00000020739.1"/>
        <property type="gene ID" value="ENSGAGG00000015270.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15097">
        <property type="entry name" value="7tmA_Gal2_Gal3_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000092">
        <property type="entry name" value="Galanin receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000405">
        <property type="entry name" value="Galanin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF31">
        <property type="entry name" value="LOC100125055 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00663">
        <property type="entry name" value="GALANINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="86"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="417" mass="47074" checksum="0A2139CADA85979C" modified="2019-05-08" version="1">MESHPPSPVRQSQGLWLLSSSLRSLPFLSLCTHQVSCISPEGRMTEHEDFASLAGYWNASDSSQFSPASVIIPVVFSLIFLLGTVGNSLVLAVLLRNGQMGHNTTNLFILNLSLADFFFIIFCVPFQATIYSLEGWVFGSFMCKAVHFFIYLTMYASSFTLAAVSVDRYLAIRYPLSSRELRTPCNAVVALAVIWGLSVVFAGPYLSYYDLIEWESSYICMPGWEEWKRKIIDTSTFAFGYVIPVLIVSLSYTRTIKYLWTAVDPLEDMSESKKAKRKVTKMIIIVTILFCLCWLPYHVVILRYLYGDFPFNQATYAFRLLSHCMAYANSCLNPIVYALVSKHFRKGFKKVFSCLLRKKARNKVHVVHAAHMVPGFEVGSTEVSQMNEENNRQQDGCELDAGSLMVQSGSSRPLHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I041</accession>
    <name>A0A452I041_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I041"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020852"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023747.1">
        <property type="protein sequence ID" value="ENSGAGP00000020852.1"/>
        <property type="gene ID" value="ENSGAGG00000015339.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019396">
        <property type="entry name" value="TM_Fragile-X-F-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13568">
        <property type="entry name" value="FAM11A, B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13568:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 185A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10269">
        <property type="entry name" value="Tmemb_185A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="350" mass="40760" checksum="581ADB74D27C0B41" modified="2019-05-08" version="1">MNLRGLFQDFNPSKFLIYACLLLFSVLLSLRLDDKIQWSYWAVFAPIWLWKLMVIVGASVGTGVWARNPQYRAEGETCVEFKAMLIAVGIHLLLLMFEVLVCDRIERGTHFWLLVFMPLFFVSPVSVAACVWGFRHDRSLELEILCSVNILQFIFIALRLDEIIRWPWLVVCVPLWILMSFLCLVVLYYIVWSVLFLRSMDVIAEQRRTHITMAISWMTIVVPLLTFEILLVHRLDGHNAFSYIPIFVPLWLSLITLMATTFGQKGGNHWWFGIRKDFCQFLLELFPFLREYGNISYDLHHEDNEELEETPVPEPPKIAPMFRKKTGVVITQSPGKYVIPPPKLSIDMPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I096</accession>
    <name>A0A452I096_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase subunit 4L</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I096"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020865"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023763.1">
        <property type="protein sequence ID" value="ENSGAGP00000020865.1"/>
        <property type="gene ID" value="ENSGAGG00000015347.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="64" mass="7074" checksum="F44B95B7A6184266" modified="2019-05-08" version="1">MTPTHFSYYSAFIISITGLSLHRTHLISTLLCLEGIILMGLSLLVASSRTHGSDRLQNLNLLEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0G6</accession>
    <name>A0A452I0G6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine rich repeat containing 72</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I0G6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021001"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023922.1">
        <property type="protein sequence ID" value="ENSGAGP00000021001.1"/>
        <property type="gene ID" value="ENSGAGG00000015433.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042655">
        <property type="entry name" value="LRC72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46759">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46759:SF1">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="31329" checksum="9BB0B0AC07C7366A" modified="2019-05-08" version="1">MAASRSGSSCRAIENQLKICGFKRDMDVSVLYLAGQGLKEVPSLSRFQILRYLWLNNNKIQKLTFLINNYRLTELYLNNNELIDIAGALKHLHALQILLLHNNQLKNLDTTVKELKGMISLQTLNLFHNPLSQDPGYRLYVTYFLPSVQLLDRKKVTQKERESALHIYNHERSRVLQSVAFGKRIDMPVVAKTISTRSTPCAKSLLLSPGYEVANHINKVPFENPEDAVFVRAMKRSIMEFSSVDWNKIPTCLGIHLENKPEDPHEKLTVKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0X0</accession>
    <name>A0A452I0X0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I0X0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021153"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024090.1">
        <property type="protein sequence ID" value="ENSGAGP00000021153.1"/>
        <property type="gene ID" value="ENSGAGG00000015515.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005402">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF3">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL SUBUNIT BETA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01580">
        <property type="entry name" value="KCNAB3CHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="3"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="213" mass="23883" checksum="0BB7BDC6DAC293F1" modified="2019-05-08" version="1">VFANRMDVNSPMEEIVRAMTYVINQGMAMYWGTSRWSAMEIMEAYSVARQFNLVPPVCEQAEYHMFQRDKVESQLPELYHKIGVGAVTWSPLACGLITGKYEERVPENCRAAIKGYQWLKEKLQSDDGRKQQAKIKELQPIAERLGCTVAQLAIAWCLRSEGVSSVLLGVSSTEQLIENLGAIHVLAQLTPPVIQEIDALLGNKPNTKKESRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4J4</accession>
    <name>A0A452I4J4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I4J4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022481"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025611.1">
        <property type="protein sequence ID" value="ENSGAGP00000022481.1"/>
        <property type="gene ID" value="ENSGAGG00000016488.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF176">
        <property type="entry name" value="CORIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="14"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="113" mass="12418" checksum="095AB98C82B64626" modified="2019-05-08" version="1">MECTSEKSMGSPRIVGGNDAKNGSWPWQVSIREGSNHICGGSLIAESWVVSAAHSITVQLFPNCHVPEAAASQCQVNHPLHPRLGLVPVEWLRPGPGSWAPWRRSRSRLQTVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6P8</accession>
    <name>A0A452I6P8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sulfotransferase</fullName>
            <ecNumber evidence="5">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452I6P8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023206"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026441.1">
        <property type="protein sequence ID" value="ENSGAGP00000023206.1"/>
        <property type="gene ID" value="ENSGAGG00000016851.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000433">
        <property type="entry name" value="Estrogen sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="6">
        <location>
            <begin position="45"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="302" mass="35450" checksum="15B61A1AC5A61878" modified="2019-05-08" version="1">MSKEQTKALTLEKEGHPELGEVEGIPLVQVTCNEWQRIQSFQARPDDLLICTYPKAGTTWIQEIVDMIQQDGDLEKCKRAPVHHRHPFIEWVRPPQPSGVEQAEAMPSPRTLKTHLPVKLLPPSFWEQQCKFLYVARNAKDCMVSYYHFQRMNKVLPDPGPWHEYFEMFMAGKVGWGPWHDHVKGWWKVKDMHCVLFLFYEDMKKDLKREIQKVAQFLGKNLDQELLDKIVYHTSFDVMKDNPMANRSGVPRSFMDLSISPFMRKGTVGDWKNHFTVAQNERFEEDYEKKMAGTNLTFCMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7A4</accession>
    <name>A0A452I7A4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I7A4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026789.1">
        <property type="protein sequence ID" value="ENSGAGP00000023504.1"/>
        <property type="gene ID" value="ENSGAGG00000017233.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001234">
        <property type="term" value="P:negative regulation of apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF35">
        <property type="entry name" value="PROTEIN LIFEGUARD 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="101" mass="11448" checksum="7D3213E962A91EF6" modified="2019-05-08" version="1">LCGSHGDLSPSRWQVDFTSCTGLFCVLGIVVVRTSILRICSGMTDALPCLWSPQFLAYDTQLLVGNKQYGLSPEEYIFDALEIYVDIVYIFLFLLRFLGWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I860</accession>
    <name>A0A452I860_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TM7S3/TM198-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I860"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023829"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027143.1">
        <property type="protein sequence ID" value="ENSGAGP00000023829.1"/>
        <property type="gene ID" value="ENSGAGG00000017437.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043069">
        <property type="term" value="P:negative regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025256">
        <property type="entry name" value="TM7S3/TM198-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042502">
        <property type="entry name" value="TM7SF3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15937">
        <property type="entry name" value="TRANSMEMBRANE 7 SUPERFAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15937:SF3">
        <property type="entry name" value="TRANSMEMBRANE 7 SUPERFAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25992">
        <property type="entry name" value="Ig_TM7SF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13886">
        <property type="entry name" value="TM7S3_TM198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019475203" description="TM7S3/TM198-like domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="483"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="TM7S3/TM198-like" evidence="7">
        <location>
            <begin position="297"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="568" mass="64237" checksum="05A12BF784871631" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8D5</accession>
    <name>A0A452I8D5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CutA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I8D5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023916"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027237.1">
        <property type="protein sequence ID" value="ENSGAGP00000023916.1"/>
        <property type="gene ID" value="ENSGAGG00000017491.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.120:FF:000005">
        <property type="entry name" value="CUTA isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004323">
        <property type="entry name" value="Ion_tolerance_CutA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419">
        <property type="entry name" value="DIVALENT CATION TOLERANCE CUTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419:SF1">
        <property type="entry name" value="PROTEIN CUTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03091">
        <property type="entry name" value="CutA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019233486" evidence="4">
        <location>
            <begin position="21"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="140" mass="15544" checksum="511BEBF7BA3207F4" modified="2019-05-08" version="1">MKAACCVLLLSLLMVSLLRTVYQRFSSMAAEAYTPGSLSAAFVTCPNEKVGKEIARAMVEKHLAACVNIMPQITSIYEWKGKIEEDREVLLMIKTRSSQIPALTDFVRSVHPYEVAEVIALPIQQGNAPYLQWVGETVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8I7</accession>
    <name>A0A452I8I7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="21">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2), but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man(5-6)GlcNAc(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="21"/>
    <dbReference type="AlphaFoldDB" id="A0A452I8I7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023975"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027301.1">
        <property type="protein sequence ID" value="ENSGAGP00000023975.1"/>
        <property type="gene ID" value="ENSGAGG00000017531.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000010">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF55">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MANNOSYL-OLIGOSACCHARIDE 1,2-ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14 21" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="111"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="142"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="18">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="432"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="18">
        <location>
            <position position="539"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="568"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="657"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="496"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00053655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="668" mass="75760" checksum="1C770C134C71DFCE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8K7</accession>
    <name>A0A452I8K7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein phosphatase inhibitor 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I8K7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023992"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027323.1">
        <property type="protein sequence ID" value="ENSGAGP00000023992.1"/>
        <property type="gene ID" value="ENSGAGG00000017549.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007062">
        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398:SF20">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY INHIBITOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="86" mass="9786" checksum="98D8002149E3D468" modified="2019-05-08" version="1">MEQVLKESTLNHLEERKVIRNSQHGLTKGKLCLTNLIAFDDEITGSVDEGKASQKWDEMNILATYHPAGKDYGLMKIDEPSTPYHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9N0</accession>
    <name>A0A452I9N0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription factor JunD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Transcription factor AP-1 subunit JunD</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. Jun subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I9N0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024380"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027749.1">
        <property type="protein sequence ID" value="ENSGAGP00000024380.1"/>
        <property type="gene ID" value="ENSGAGG00000017814.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14696">
        <property type="entry name" value="bZIP_Jun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000012">
        <property type="entry name" value="Putative transcription factor AP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050946">
        <property type="entry name" value="AP-1_TF_bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005643">
        <property type="entry name" value="JNK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002112">
        <property type="entry name" value="Leuzip_Jun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462">
        <property type="entry name" value="JUN TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462:SF7">
        <property type="entry name" value="TRANSCRIPTION FACTOR JUND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03957">
        <property type="entry name" value="Jun"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00043">
        <property type="entry name" value="LEUZIPPRJUN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="11">
        <location>
            <begin position="209"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="207"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="289" mass="30902" checksum="DD31580ED836F890" modified="2019-05-08" version="1">METPFYHDDVLSGFGSGSAPSRGSSSGLARPFPGGSMMKKDALALSDQVVATLKAPGQALHQLRGAAGGGGEGPALLSSAELGLLKLGSPELERLIIQSNGLVTTTPTSSQFLYPKVAASEEQEFAEGFPPPPPPEPPVYANLSSYPGTTVNYATETVPYPPPPGLGGAPPHPRLQPPPLKDEPQIVPEVPSFGESPPLSPIDMDTQERIKAERKRLRNRIAASKCRKRKLERISRLEEKVKSLKSQNTELASTASLLREQVAQLKQKVLSHVNSGCQLLPQQHQVPAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAB3</accession>
    <name>A0A452IAB3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger protein 777</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000024464"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027842.1">
        <property type="protein sequence ID" value="ENSGAGP00000024464.1"/>
        <property type="gene ID" value="ENSGAGG00000017871.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000003">
        <property type="entry name" value="Zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001210">
        <property type="entry name" value="zinc finger protein 777"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003655">
        <property type="entry name" value="aKRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381:SF269">
        <property type="entry name" value="ZINC FINGER PROTEIN 398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50806">
        <property type="entry name" value="KRAB_RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB-related" evidence="13">
        <location>
            <begin position="104"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="12">
        <location>
            <begin position="107"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="385"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="415"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="443"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="471"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="60555" checksum="38E6DDDB14B7E724" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB08</accession>
    <name>A0A452IB08_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NEDD8-conjugating enzyme UBC12</fullName>
            <ecNumber evidence="7">2.3.2.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">NEDD8 carrier protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">NEDD8-conjugating enzyme Ubc12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin-conjugating enzyme E2 M</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.34" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452IB08"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024915"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028362.1">
        <property type="protein sequence ID" value="ENSGAGP00000024915.1"/>
        <property type="gene ID" value="ENSGAGG00000018214.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061654">
        <property type="term" value="F:NEDD8 conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23794">
        <property type="entry name" value="UBCc_UBE2F_UBE2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000239">
        <property type="entry name" value="NEDD8-conjugating enzyme Ubc12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="16">
        <location>
            <begin position="29"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="13">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="183" mass="20943" checksum="8E05E3B4FC22B203" modified="2019-05-08" version="1">MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCEIDFSDQDDLLNFKLVICPDEGFYKGGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB29</accession>
    <name>A0A452IB29_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IB29"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024894"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028337.1">
        <property type="protein sequence ID" value="ENSGAGP00000024894.1"/>
        <property type="gene ID" value="ENSGAGG00000018195.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22177">
        <property type="entry name" value="F-box_FBXO46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039594">
        <property type="entry name" value="FBXO34/46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001562">
        <property type="entry name" value="Znf_Btk_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16271">
        <property type="entry name" value="F-BOX ONLY PROTEIN 34/46 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16271:SF10">
        <property type="entry name" value="F-BOX ONLY PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51113">
        <property type="entry name" value="ZF_BTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="F-box" evidence="4">
        <location>
            <begin position="529"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="37"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="111"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="350"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="469"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="662" mass="72060" checksum="E0ED17B60F61C582" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB40</accession>
    <name>A0A452IB40_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IB40"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024729"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028139.1">
        <property type="protein sequence ID" value="ENSGAGP00000024729.1"/>
        <property type="gene ID" value="ENSGAGG00000018052.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072655">
        <property type="term" value="P:establishment of protein localization to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000003">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="14">
        <location>
            <begin position="64"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="139" mass="15845" checksum="7DCEF23BBF35F2CD" modified="2019-05-08" version="1">MFRACAVRTISLLSMRGEVIIPLRASCWQTREGRSRGRAVCPERGMEASSPAGLSATDPQLQHFIEVETQKQRFQQLVHQMTELCWEKCMDKPGPKLDSRAETCFVNCVERFIDTSQFILNRLEQTQKSKSAFSESLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBL1</accession>
    <name>A0A452IBL1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IBL1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025143"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028631.1">
        <property type="protein sequence ID" value="ENSGAGP00000025143.1"/>
        <property type="gene ID" value="ENSGAGG00000018398.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="48"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="120" mass="13174" checksum="F93B68AD2D1C9B43" modified="2019-05-08" version="1">MMAAAPIQQNGTHTGVPIDLDPPDSRKRPLEAPPEAGSTKRTNTGEDGQYFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGRCKYYFLSTLFHCQAEVDHLFSMERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBT1</accession>
    <name>A0A452IBT1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Gamma-synuclein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases. May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">May be a centrosome-associated protein. Interacts with MYOC; affects its secretion and its aggregation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the synuclein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IBT1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025209"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028700.1">
        <property type="protein sequence ID" value="ENSGAGP00000025209.1"/>
        <property type="gene ID" value="ENSGAGG00000018431.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903136">
        <property type="term" value="F:cuprous ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.700:FF:000002">
        <property type="entry name" value="Gamma-synuclein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.700">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001058">
        <property type="entry name" value="Synuclein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002462">
        <property type="entry name" value="Synuclein_gamma"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13820:SF10">
        <property type="entry name" value="GAMMA-SYNUCLEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13820">
        <property type="entry name" value="SYNUCLEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01387">
        <property type="entry name" value="Synuclein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01214">
        <property type="entry name" value="GSYNUCLEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01211">
        <property type="entry name" value="SYNUCLEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118375">
        <property type="entry name" value="Synuclein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="137"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="181" mass="19353" checksum="BA14779B4A176282" modified="2019-05-08" version="1">MHTFDMSDDACVKMLHHSAAAELSSVYSNTKRSCREYSSKQARMDVFKKGFSIAKEGVVAAAEKTKQGVTEAAEKTKEGVMYVGTKTKEGVVHSVSSVAEKTKEQANVVGEAVVASVNTVARQTVEGAETIVTTTGIVKKDELSHPEHPTEPAAAEEEPPAEAIKASGEGENKPINQFQDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IC21</accession>
    <name>A0A452IC21_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Interphotoreceptor matrix proteoglycan 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Sialoprotein associated with cones and rods proteoglycan</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Photoreceptor inner segment membrane</location>
            <topology evidence="15">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Photoreceptor outer segment membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
            <location evidence="2">Interphotoreceptor matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IC21"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028737.1">
        <property type="protein sequence ID" value="ENSGAGP00000025241.1"/>
        <property type="gene ID" value="ENSGAGG00000018446.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033165">
        <property type="term" value="C:interphotoreceptor matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.960:FF:000002">
        <property type="entry name" value="Interphotoreceptor matrix proteoglycan 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039861">
        <property type="entry name" value="IMPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12199">
        <property type="entry name" value="INTERPHOTORECEPTOR MATRIX PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12199:SF4">
        <property type="entry name" value="INTERPHOTORECEPTOR MATRIX PROTEOGLYCAN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00200">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1047"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="domain" description="SEA" evidence="20">
        <location>
            <begin position="43"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="SEA" evidence="20">
        <location>
            <begin position="844"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1166" mass="129134" checksum="7FAEC0437126D760" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ID26</accession>
    <name>A0A452ID26_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Alpha-aminoadipic semialdehyde synthase, mitochondrial</fullName>
            <ecNumber evidence="6">1.5.1.8</ecNumber>
            <ecNumber evidence="7">1.5.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">LKR/SDH</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Bifunctional enzyme that catalyzes the first two steps in lysine degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-saccharopine + NAD(+) + H2O = (S)-2-amino-6-oxohexanoate + L-glutamate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24520"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="EC" id="1.5.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:24521"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-saccharopine + NADP(+) + H2O = L-lysine + 2-oxoglutarate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19373"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.8"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:19375"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 1/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 2/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">In the C-terminal section; belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AlaDH/PNT family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.8" evidence="6"/>
    <dbReference type="EC" id="1.5.1.9" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452ID26"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025734"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029275.1">
        <property type="protein sequence ID" value="ENSGAGP00000025734.1"/>
        <property type="gene ID" value="ENSGAGG00000018782.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00835"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047131">
        <property type="term" value="F:saccharopine dehydrogenase (NAD+, L-glutamate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047130">
        <property type="term" value="F:saccharopine dehydrogenase (NADP+, L-lysine-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12189">
        <property type="entry name" value="LKR_SDH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1870.10:FF:000001">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000008">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000087">
        <property type="entry name" value="alpha-aminoadipic semialdehyde synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000072">
        <property type="entry name" value="Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1870.10">
        <property type="entry name" value="Domain 3, Saccharopine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01002">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal" evidence="22">
        <location>
            <begin position="45"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding" evidence="21">
        <location>
            <begin position="215"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="944" mass="104584" checksum="D070F229897B3CCE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ID54</accession>
    <name>A0A452ID54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hedgehog acyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the membrane-bound acyltransferase family. HHAT subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ID54"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025638"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029172.1">
        <property type="protein sequence ID" value="ENSGAGP00000025638.1"/>
        <property type="gene ID" value="ENSGAGG00000018722.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016409">
        <property type="term" value="F:palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051085">
        <property type="entry name" value="MB_O-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285:SF20">
        <property type="entry name" value="PROTEIN-CYSTEINE N-PALMITOYLTRANSFERASE HHAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15017" checksum="B53C1D2B5579A003" modified="2019-05-08" version="1">MGGSHGSVFGILFSTAITFSFVSYWHGGHNYLWFWAALNWLGVIAENGVKRLLSVSLVRDLIDRFFSPRICRRLHAALAAVSTSMLILSNLVFLGGNQVGKIYWNRIFVEGKLFRHALGDKAFECCFEIERSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IEY3</accession>
    <name>A0A452IEY3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dual specificity phosphatase 28</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate (in vitro). Has almost no detectable activity with phosphotyrosine, even less activity with phosphothreonine and displays complete lack of activity with phosphoserine. The poor activity with phosphotyrosine may be due to steric hindrance by bulky amino acid sidechains that obstruct access to the active site.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 16">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452IEY3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026389"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030001.1">
        <property type="protein sequence ID" value="ENSGAGP00000026389.1"/>
        <property type="gene ID" value="ENSGAGG00000019239.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14574">
        <property type="entry name" value="DUSP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000107">
        <property type="entry name" value="Dual specificity phosphatase 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020420">
        <property type="entry name" value="Atypical_DUSP_subfamB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052103">
        <property type="entry name" value="Dual_spec_Phospatases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45961:SF7">
        <property type="entry name" value="DUAL SPECIFICITY PHOSPHATASE 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45961">
        <property type="entry name" value="IP21249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01910">
        <property type="entry name" value="ADSPHPHTASEB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="3"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="64"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="159" mass="18163" checksum="4FB6754E36E3C5BE" modified="2019-05-08" version="1">MLQLCKITDSLLISNSRSACNKELLTQEGVTFCINVSRQQPFPGLHHIRSLRVPVFDDPLEDLYKYFEQCNNAIEDTVRSGGKCLVYCKNGRSRSAAICTAYLMRHQNLTLKDAFESVKTARPGVEPNTGFWTQLQRYEEYLQTQHQLGHSSEKMISSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFV8</accession>
    <name>A0A452IFV8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ADP-ribosylation factor-related protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Trans-Golgi-associated GTPase that regulates protein sorting. Controls the targeting of ARL1 and its effector to the trans-Golgi. Required for the lipidation of chylomicrons in the intestine and required for VLDL lipidation in the liver.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with SYS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IFV8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026780"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030437.1">
        <property type="protein sequence ID" value="ENSGAGP00000026780.1"/>
        <property type="gene ID" value="ENSGAGG00000019506.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04160">
        <property type="entry name" value="Arfrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000509">
        <property type="entry name" value="ADP-ribosylation factor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="143"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="210" mass="23710" checksum="E3D7D6FC2F2ADEEC" modified="2019-05-08" version="1">MMGPSARSRMYTLLSGLYKYMFRRDEYCILILGLDNAGKTTFLEQTKTRFNRTYKGMSLSKITTTVGLNIGTIDVGKARLMFWDLGGQDELQSLWDKYYAESHGVIYVIDSTDEERLSESKRAFEKVVTSEALEGVPILVLANKQDVETCLSIPDIKTAFSDCINKIGRRDCLTQACSALTGNGVNEGIEWMVKCVVRNIHRPPRQKDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IH30</accession>
    <name>A0A452IH30_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BDH protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IH30"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027293"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031004.1">
        <property type="protein sequence ID" value="ENSGAGP00000027293.1"/>
        <property type="gene ID" value="ENSGAGG00000019846.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313:SF43">
        <property type="entry name" value="D-BETA-HYDROXYBUTYRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY 9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="36006" checksum="EEC5142ADFC9028F" modified="2019-05-08" version="1">PRAVCGWKPLSPLTGGAATMWEAPALLLLLLLVPLALLARLPRQAALQPAGRAVLITGCDSGFGHLLALRLHRLGFTVFAGCLCPGGEGARRLLREAASGSGRLRVLPLDVTRERDVAAAKEFVLSNLPETGFWGLVNNAGISTFGETGWLPMEKYEKFVDVNLLGCIRTTLAFLPLLRKYKGRMVFMSSVTAYFTLGNGIYSMTKAAIEKFCDALRLEMKKFGVLVCVIQPGNYARSTNVQPPVNAEQIWNELSEEEKAVYSKEYVQERSDFFNNILKEGCTTGHEVVDAMVDALMSPAPKARYMVAKLKEKLLVFISIFPYIRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452II15</accession>
    <name>A0A452II15_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452II15"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031213.1">
        <property type="protein sequence ID" value="ENSGAGP00000027478.1"/>
        <property type="gene ID" value="ENSGAGG00000019970.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01345">
        <property type="entry name" value="CERVTRCPTASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="293" mass="33954" checksum="19C240F1026C1677" modified="2019-05-08" version="1">MGMNMKVVRWIRNWLKGRLQRVVLKGELSGWKEVTSGVPQGSVLGPILFNLFITDLGTKSGNVLIKFADDTKLGGIANTEKDRDTIQEDLNHLVNWSNRNRMKYNSEKCKVMHLGINNKNFGYTLGAHQLEATEEEKDLGVLVDSRMTMSRQCDTAVKKANAILGCIRRGISCKDKEVLVPLYKALVRPHLEYCVQFWCPMFKKDEFKLEQVQRRATRMIRGMEKLPYERRLKELGLFSLAKRRLRGDMLALYKYIRGVNVREGEELFKFSTNVGTRTNGYKLDIRKFRLEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIC4</accession>
    <name>A0A452IIC4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD164 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IIC4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027611"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031367.1">
        <property type="protein sequence ID" value="ENSGAGP00000027611.1"/>
        <property type="gene ID" value="ENSGAGG00000020064.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007947">
        <property type="entry name" value="CD164_MGC24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11337:SF11">
        <property type="entry name" value="CD164 SIALOMUCIN-LIKE 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11337">
        <property type="entry name" value="MUCIN/PORIMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05283">
        <property type="entry name" value="MGC-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019506161" evidence="10">
        <location>
            <begin position="29"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="170" mass="18261" checksum="B8A0AD8C707D94F4" modified="2019-05-08" version="1">MGAPRTWARRAALCCALLCAPWLPLGWAGECKQLEPCERCIEGDASQNITGCVWMRCGATEEPGPGSCIGKGDATKETCSIYNVTAVCPAQRSPTKEPPRASTKQPEILTPGMTAGPPLTTTPEFRPPGFDSASFVGGIVLVLSVQAVFYFIVKFLKSKDSTYQTLEENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJV8</accession>
    <name>A0A452IJV8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptor 20</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Orphan receptor with constitutive G(i) signaling activity that activate cyclic AMP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJV8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028161"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032001.1">
        <property type="protein sequence ID" value="ENSGAGP00000028161.1"/>
        <property type="gene ID" value="ENSGAGG00000020466.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15163">
        <property type="entry name" value="7tmA_GPR20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000243">
        <property type="entry name" value="G protein-coupled receptor 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF7">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="67"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="360" mass="40589" checksum="A0A7679DF607C196" modified="2019-05-08" version="1">MHAASSPQVPALNLLNSTRAPNSSSSDEDNLFHKFIHMDQELYQDYYSLWIALMVVNGIIFLVGVVLNSLALYVFCFRTKTKTTSVIYTINLIVTDLLVGFSLPTRIIMYYTAGDCMKCSFVHIFGYFVNMYCSILFLTCICIDRYLAIVQVEASRKWRNPSCAKGICVFIWVFATVVTFSILIMAITFAPCCLAKIFALMVCEYFFPLIIITFFTTRIMCALSKPSLMHQSRERRMRAVQLLTTVLIIFMICFTPFHVRQVAISINPDIPHEISLIVYHVTVTLSSLNSCMDPIVYCFVTNNFQSTMKNIFRKSEPEQTSGDIISMNKSSKGSGTNAIIAFSNTIGSPLSLPSTNNTQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IN18</accession>
    <name>A0A452IN18_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IN18"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029282"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033259.1">
        <property type="protein sequence ID" value="ENSGAGP00000029282.1"/>
        <property type="gene ID" value="ENSGAGG00000021184.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042756">
        <property type="entry name" value="Sel-1L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44444">
        <property type="entry name" value="PROTEIN SEL-1 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44444:SF1">
        <property type="entry name" value="PROTEIN SEL-1 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019543685" evidence="3">
        <location>
            <begin position="29"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1028"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1057"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1067"/>
            <end position="1078"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1100" mass="126795" checksum="0E06436372681C3F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPH4</accession>
    <name>A0A452IPH4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphodiesterase</fullName>
            <ecNumber evidence="10">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.4.53"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452IPH4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029886"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033938.1">
        <property type="protein sequence ID" value="ENSGAGP00000029886.1"/>
        <property type="gene ID" value="ENSGAGG00000021546.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00762">
        <property type="reaction ID" value="UER00747"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006198">
        <property type="term" value="P:cAMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000001">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040844">
        <property type="entry name" value="PDE4_UCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18100">
        <property type="entry name" value="PDE4_UCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="12">
        <location>
            <begin position="314"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="390"/>
            <end position="394"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="548"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="548"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="599"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="616" mass="70445" checksum="229198E5B54E9472" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPH6</accession>
    <name>A0A452IPH6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipase A2-like central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IPH6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029744"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033773.1">
        <property type="protein sequence ID" value="ENSGAGP00000029744.1"/>
        <property type="gene ID" value="ENSGAGG00000021473.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00085">
        <property type="entry name" value="PA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018981167" description="Phospholipase A2-like central domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase A2-like central" evidence="2">
        <location>
            <begin position="20"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="127" mass="14236" checksum="3BF1341CBF978BF6" modified="2019-05-08" version="1">MNSLLIFAVFIAYVSMAHGSALVFNKMIKAATGKCAMFSYYRCYCGLGGKETALDVTERSSIILSSYRKHFSGQEPATTCSDQSQCRRVVYLCHRSTALCFQKILVIYSKSCQFYSNRQCKGSKARC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQM4</accession>
    <name>A0A452IQM4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histidine decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.22" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452IQM4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030410"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034509.1">
        <property type="protein sequence ID" value="ENSGAGP00000030410.1"/>
        <property type="gene ID" value="ENSGAGG00000021898.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004398">
        <property type="term" value="F:histidine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042423">
        <property type="term" value="P:catecholamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001694">
        <property type="term" value="P:histamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006548">
        <property type="term" value="P:L-histidine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1340.10:FF:000001">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000025">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000018">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1340.10">
        <property type="entry name" value="dopa decarboxylase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF68">
        <property type="entry name" value="HISTIDINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="661" mass="74192" checksum="1FAD4E7EA4A5973C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRI3</accession>
    <name>A0A452IRI3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAM86 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. EEF2KMT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IRI3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030472"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034577.1">
        <property type="protein sequence ID" value="ENSGAGP00000030472.1"/>
        <property type="gene ID" value="ENSGAGG00000021945.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029426">
        <property type="entry name" value="FAM86_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF130">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EEF2KMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14904">
        <property type="entry name" value="FAM86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAM86 N-terminal" evidence="5">
        <location>
            <begin position="14"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="343" mass="38575" checksum="2629C0928601659F" modified="2019-05-08" version="1">MDPAAEEARHLGLRFQRGFLAGRLLRAFPWAELEKKLKSSSDALLLLDILQKTIRHPLCMKYPPSVKYRRCFISELIKKHESIATEPLDELYDELAEILNKEESTNCYKSYLLPTGDSVTLSETVAIISQGTTGLVTWDAALYLVEWAIENSAIFSNRTILELGSGIGLTGIAVCKTCNPKAYVFSDYHHCVLEQLRENIHLNGFVLESETGNHTKMQSQNQRTEVMDLQGPKITVAELDWDSVTKEQLSELQVDVVIAADVVYDPQITLSLIGVLQQLSTSETIGNPPEIYIAFTVRNPDTYHLFQTELGKVGIGWQVVPTHTKNLFLYDLHSDIILLKLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRJ0</accession>
    <name>A0A452IRJ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trinucleotide repeat-containing gene 6C protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GW182 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000030617"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034740.1">
        <property type="protein sequence ID" value="ENSGAGP00000030617.1"/>
        <property type="gene ID" value="ENSGAGG00000022043.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035195">
        <property type="term" value="P:miRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060213">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12713">
        <property type="entry name" value="RRM_TNRC6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14283">
        <property type="entry name" value="UBA_TNR6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000011">
        <property type="entry name" value="trinucleotide repeat-containing gene 6A protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000027">
        <property type="entry name" value="Trinucleotide repeat-containing gene 6C protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019486">
        <property type="entry name" value="Argonaute_hook_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052068">
        <property type="entry name" value="GW182_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026805">
        <property type="entry name" value="GW182_M_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041917">
        <property type="entry name" value="TNR6C_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032226">
        <property type="entry name" value="TNRC6_PABC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034927">
        <property type="entry name" value="TNRC6C_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13020">
        <property type="entry name" value="TRINUCLEOTIDE REPEAT-CONTAINING GENE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13020:SF9">
        <property type="entry name" value="TRINUCLEOTIDE REPEAT-CONTAINING GENE 6C PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10427">
        <property type="entry name" value="Ago_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12938">
        <property type="entry name" value="M_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16608">
        <property type="entry name" value="TNRC6-PABC_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="UBA" evidence="11">
        <location>
            <begin position="1009"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="49"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="238"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="310"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="857"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1152"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1292"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1471"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1506"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1569"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1709"/>
            <end position="1737"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1258"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="238"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="265"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="429"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="447"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="465"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="496"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="525"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="545"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="563"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="621"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="662"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="717"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="745"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="860"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="890"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="977"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1323"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1344"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1362"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1509"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1591"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1715"/>
            <end position="1736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1803" mass="188433" checksum="3A59884E235FB489" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IT13</accession>
    <name>A0A452IT13_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1</fullName>
            <ecNumber evidence="4">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Radical S-adenosyl methionine and flavodoxin domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">tRNA wybutosine-synthesizing protein 1 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">tRNA-yW-synthesizing protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TYW1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452IT13"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031166"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035343.1">
        <property type="protein sequence ID" value="ENSGAGP00000031166.1"/>
        <property type="gene ID" value="ENSGAGG00000022393.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000196">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="21">
        <location>
            <begin position="84"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="22">
        <location>
            <begin position="405"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="252"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="330"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="271"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="737" mass="83991" checksum="15705078242D6AA7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWE3</accession>
    <name>A0A452IWE3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IWE3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032452"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036797.1">
        <property type="protein sequence ID" value="ENSGAGP00000032452.1"/>
        <property type="gene ID" value="ENSGAGG00000023219.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033242">
        <property type="entry name" value="PPP1R17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15387">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15387:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="158" mass="18170" checksum="273FE26249951B58" modified="2019-05-08" version="1">MMSTEYVQPLDIPEDRLDKRDSHCNRLDDLSEHFIKNCDLKKKPRKGKNIQTSQNSELEQKKPRRKDTPALHTPPPIPGILSDFSDHLIKRCDITEKPQKGKASSVSQITDQEQKKPRRKDTPALHIPPLIPGTYLLKEEKQTVIVEDDEKDGEKTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IXF5</accession>
    <name>A0A452IXF5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAM234A/B beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FAM234 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IXF5"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037106.1">
        <property type="protein sequence ID" value="ENSGAGP00000032742.1"/>
        <property type="gene ID" value="ENSGAGG00000023396.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000037107.1">
        <property type="protein sequence ID" value="ENSGAGP00000032743.1"/>
        <property type="gene ID" value="ENSGAGG00000023396.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055409">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045232">
        <property type="entry name" value="FAM234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21419">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21419:SF7">
        <property type="entry name" value="PROTEIN FAM234A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23727">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="FAM234A/B beta-propeller" evidence="7">
        <location>
            <begin position="89"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="564" mass="62921" checksum="25A97E946CE1C69B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IXI5</accession>
    <name>A0A452IXI5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IXI5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032761"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037125.1">
        <property type="protein sequence ID" value="ENSGAGP00000032761.1"/>
        <property type="gene ID" value="ENSGAGG00000023409.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033316">
        <property type="entry name" value="RBBP8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15107:SF3">
        <property type="entry name" value="RBBP8 N-TERMINAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15107">
        <property type="entry name" value="RETINOBLASTOMA BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="484" mass="54135" checksum="3FB9D4E2A84442A9" modified="2019-05-08" version="1">MTSQRIANQLHGTIALVRPGSRLCLPEKISARRATSPPARKISLFLERERSPSHEACLRTNKPDSHKATSSYGTLQLTTRQEQLCLLNKHFSLHQFGLRSNSASADRDSSLPSHLLQARETEDRTRLQEEWEDQAAILELPGAVVYMRDQHLEGRLPFLKHREGLQYLLAQEHQQGFRARTEGNQNATPYPAAPEERPLSPQLTTGCKEERSYLEEPVEGKVVWINRDNVEQREKVEAARDCLLDTPLDLSDYGRRSESLKPASWHKSPKQREVDSPSPIPMESPTHEIGQSSSVLPRAEHENSHLACSQLPAAKLLQIGNKTEQFSAKEQEAPVVPLVPSSQGQPAGKLTSCDPATDSETRVRVSPRAQNREPGAQTEEDDAESVKQDSDEPDTSDSEAAATYEHETHHEEHAEGKHLCTVDKDEVLQKKRKRGQDPETKAHKKSVRGRRKIKAPEAPADAAEITKETEHNSPACGNDVFEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0Y6</accession>
    <name>A0A452J0Y6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Myelin transcription factor 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYT1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J0Y6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033979"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038479.1">
        <property type="protein sequence ID" value="ENSGAGP00000033979.1"/>
        <property type="gene ID" value="ENSGAGG00000024142.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.320.30:FF:000001">
        <property type="entry name" value="Myelin transcription factor 1-like, a"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.30">
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013681">
        <property type="entry name" value="Myelin_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002515">
        <property type="entry name" value="Znf_C2H2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036060">
        <property type="entry name" value="Znf_C2H2C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816:SF10">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08474">
        <property type="entry name" value="MYT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01530">
        <property type="entry name" value="zf-C2HC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103637">
        <property type="entry name" value="CCHHC domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51802">
        <property type="entry name" value="ZF_CCHHC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Myelin transcription factor 1" evidence="14">
        <location>
            <begin position="582"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="Myelin transcription factor 1" evidence="14">
        <location>
            <begin position="638"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="124"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="289"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="539"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="689"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1019"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="131"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="296"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="347"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="369"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="704"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="781"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1147" mass="127735" checksum="86A350431D2191CB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1E5</accession>
    <name>A0A452J1E5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP synthase membrane subunit DAPIT</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J1E5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034216"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038743.1">
        <property type="protein sequence ID" value="ENSGAGP00000034216.1"/>
        <property type="gene ID" value="ENSGAGG00000024304.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009125">
        <property type="entry name" value="ATPMK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34038">
        <property type="entry name" value="ATP SYNTHASE MEMBRANE SUBUNIT DAPIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34038:SF1">
        <property type="entry name" value="ATP SYNTHASE MEMBRANE SUBUNIT K, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14960">
        <property type="entry name" value="ATP_synth_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01821">
        <property type="entry name" value="DAPIT"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="13174" checksum="2093AC8930BE0443" modified="2019-05-08" version="1">MDIFQSQSSISRHVISSWKDLVVQSGEACLRGGGISQRHLACGLGVGEGHGAAVAGERAAGPATMAGHDSGTQHKFTGIQKYFNAYTMTGRRNYVIATYASIAMVILYFKLKPKKQTPAVTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J214</accession>
    <name>A0A452J214_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL22 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J214"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034431"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038982.1">
        <property type="protein sequence ID" value="ENSGAGP00000034431.1"/>
        <property type="gene ID" value="ENSGAGG00000024474.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.210:FF:000001">
        <property type="entry name" value="60S ribosomal protein L22 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002671">
        <property type="entry name" value="Ribosomal_eL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038526">
        <property type="entry name" value="Ribosomal_eL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN EL22-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01776">
        <property type="entry name" value="Ribosomal_L22e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="144" mass="16976" checksum="4BB88441881FFF36" modified="2019-05-08" version="1">MFCCCTVCPFWELAKLTFNANLQRDRKSKKSAWKFSLDLTHPVEDGIFDSGNFEQFLKEKVKVNGKTGNLGNIVHIERFKNKITVVSEKQFSKRYLKYLTKKYLKKNNLRDWLRVVASDKETYELRYFQISQDEEGSESEEGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4V0</accession>
    <name>A0A452J4V0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J4V0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035485"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040189.1">
        <property type="protein sequence ID" value="ENSGAGP00000035485.1"/>
        <property type="gene ID" value="ENSGAGG00000025229.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12908">
        <property type="entry name" value="SPRYD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035783">
        <property type="entry name" value="SPRYD3_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="21"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="422" mass="47406" checksum="9DD138C80DCA3E2A" modified="2019-05-08" version="1">MGHWHLQALRQGLTLTVIMDDLNLHYRFLNWRRRIREIREVRAVRYQERFKHILVDGDTLSYHGNSGEVGCYVAPRPLTKDNNYFEVSIVDSGVRGTIAVGLVPQYYSLDHQPGWLPDSVAYHADDGKLYNGRAKGRQFGTKCSSGDRIGCGIEPVSFEVQTAQIFFTKNGKRVGSTIMPLSPDGLFPAVGMHSLGEEVRLHLHAELGNEEDDSIMMVDSYEDEWGRLHDVKVCGTLLEYVGKGKSIIDVGLAQARRPLSTRSHYFEVEIVDPGEKCYIALGLARKDYPKNRHPGWSRGSVAYHADDGKIFHGSGVGDPFGPRCYKGDIMGCGIMFPRDYILDSEGDSDDSCDTTDLKPKLRSVRNVMYLHQEVEEEEEEEEEDGEEMEQEHEGKKVVVRAGGCAMGLGWLEGVRLCCWTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5U1</accession>
    <name>A0A452J5U1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDENN FNIP1/2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FNIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J5U1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035838"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040575.1">
        <property type="protein sequence ID" value="ENSGAGP00000035838.1"/>
        <property type="gene ID" value="ENSGAGG00000025431.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037545">
        <property type="entry name" value="DENN_FNIP1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028086">
        <property type="entry name" value="FNIP_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026156">
        <property type="entry name" value="FNIP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028085">
        <property type="entry name" value="FNIP_mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028084">
        <property type="entry name" value="FNIP_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21634:SF11">
        <property type="entry name" value="FOLLICULIN-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21634">
        <property type="entry name" value="RE13835P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14638">
        <property type="entry name" value="FNIP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14637">
        <property type="entry name" value="FNIP_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14636">
        <property type="entry name" value="FNIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02073">
        <property type="entry name" value="FOLLICULNIP1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51836">
        <property type="entry name" value="DENN_FNIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0458">Lysosome</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN FNIP1/2-type" evidence="8">
        <location>
            <begin position="38"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="601"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="705"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="773"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="874"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="914"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="608"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="633"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="881"/>
            <end position="894"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1145" mass="126472" checksum="BAD0B221FA13893F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIT7</accession>
    <name>A0A452GIT7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GIT7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001518"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001732.1">
        <property type="protein sequence ID" value="ENSGAGP00000001518.1"/>
        <property type="gene ID" value="ENSGAGG00000001227.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008327">
        <property type="term" value="F:methyl-CpG binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006346">
        <property type="term" value="P:DNA methylation-dependent constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01396">
        <property type="entry name" value="MeCP2_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.890.10:FF:000003">
        <property type="entry name" value="methyl-CpG-binding domain protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032343">
        <property type="entry name" value="MBD2/MBD3_p55-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025884">
        <property type="entry name" value="MeCpG-bd_2/3_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12396">
        <property type="entry name" value="METHYL-CPG BINDING PROTEIN, MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12396:SF12">
        <property type="entry name" value="METHYL-CPG-BINDING DOMAIN PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14048">
        <property type="entry name" value="MBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16564">
        <property type="entry name" value="MBDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00391">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MBD" evidence="10">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="253"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="253"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="287" mass="32329" checksum="FF1F946D39893837" modified="2019-05-08" version="1">MERKRWECSALPQGWKREEVTRKSGLSAGKSDVYYYSPSGKKFRSKPQLARYLGSSMDLSTFDFRTGKMLMSKMNKNRQRMRYDCSNQAKGKPDLNTALPVRQTASIFKQPVTKITNHPSNKVKSDPQKAVDQPRQLFWEKKLSGLNAFDIAEELVKTMDLPKGLQGVGPGCTDETLLSAIASALHTSTMPITGQLSAAVEKNPGVWLNTSQPLCKAFMVTDEDIRKQEELVQQVRKRLEEALMADMLAHVEEIARDGEAPSEKEGGDEEGEEEEEEQDHDQEMENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJP9</accession>
    <name>A0A452GJP9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GJP9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001919"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002180.1">
        <property type="protein sequence ID" value="ENSGAGP00000001919.1"/>
        <property type="gene ID" value="ENSGAGG00000001539.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="56" mass="6148" checksum="CE1CB723E475B5E9" modified="2019-05-08" version="1">MAPRRTQQVGAGRPSTEQAYISKVVLLGSTAVGKSSTAFRYVKNDIRETMTTVRCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GK45</accession>
    <name>A0A452GK45_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GK45"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002090"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002378.1">
        <property type="protein sequence ID" value="ENSGAGP00000002090.1"/>
        <property type="gene ID" value="ENSGAGG00000001688.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF65">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="64"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="170"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="191"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="382" mass="41444" checksum="A54C419975B64963" modified="2019-05-08" version="1">ALCIAAQEGHIDVVQILLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCTPSPVHTMEQKPLQSVSSKLQSLTIKSNSSGSTGGGDMQPTMRGLSNGPAHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHNMSFTEQIQQHSLPRSRSRQSIVSPSSTTHSLSQNHNSPSSEFEWSQVKPSLKSTKANKGGKTENSSKSGSAGKKIKQSSSSQPQVLEYEMTQFDKRIPVAKSGTSVPLKSMPAEPQCKILVPPSQQEVCRPQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTGQERGLQEVLEGYPPAETELSLKQALKLQLEGTDPSFNYKKETPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKC4</accession>
    <name>A0A452GKC4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GKC4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002076"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002363.1">
        <property type="protein sequence ID" value="ENSGAGP00000002076.1"/>
        <property type="gene ID" value="ENSGAGG00000001678.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042191">
        <property type="entry name" value="GSH1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47421">
        <property type="entry name" value="GS HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47421:SF1">
        <property type="entry name" value="GS HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="15"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="17"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="119" mass="13568" checksum="8AB22856F16641B1" modified="2025-10-08" version="2">MCLSRPGGSDTSHIPNGKRMRTAFTSTQLLELEREFSSNMYLSRLRRIEIATYLNLSEKQVKIWFQNRRVKHKKEGKGTQRNTHGGCKCTGNPGHYPRSEDEESLSPSSANEDKEISPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GL14</accession>
    <name>A0A452GL14_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000002475"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002834.1">
        <property type="protein sequence ID" value="ENSGAGP00000002475.1"/>
        <property type="gene ID" value="ENSGAGG00000001429.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF2">
        <property type="entry name" value="BETA_GAMMA CRYSTALLIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="4">
        <location>
            <begin position="1150"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="4">
        <location>
            <begin position="1251"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="4">
        <location>
            <begin position="1305"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="4">
        <location>
            <begin position="1358"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="4">
        <location>
            <begin position="1449"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="4">
        <location>
            <begin position="1527"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="4">
        <location>
            <begin position="1576"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="4">
        <location>
            <begin position="1617"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="72"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="318"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="825"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="858"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="378"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="453"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="489"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="858"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="871"/>
            <end position="880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1795" mass="199296" checksum="C47E37E405C19D38" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GL20</accession>
    <name>A0A452GL20_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peptidase M12B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GL20"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002480"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002839.1">
        <property type="protein sequence ID" value="ENSGAGP00000002480.1"/>
        <property type="gene ID" value="ENSGAGG00000001989.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF37">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="14">
        <location>
            <begin position="89"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 13">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11 13">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 13">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 13">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="83"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="100"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="138"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="226"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="583" mass="64530" checksum="BFA9A227FEBF63E0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GL26</accession>
    <name>A0A452GL26_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Serine/threonine-protein kinase Nek11</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Never in mitosis A-related kinase 11</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 28">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 28">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452GL26"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002313"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002650.1">
        <property type="protein sequence ID" value="ENSGAGP00000002313.1"/>
        <property type="gene ID" value="ENSGAGG00000001889.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000002653.1">
        <property type="protein sequence ID" value="ENSGAGP00000002316.1"/>
        <property type="gene ID" value="ENSGAGG00000001889.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08222">
        <property type="entry name" value="STKc_Nek11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001071">
        <property type="entry name" value="NIMA related kinase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000332">
        <property type="entry name" value="NIMA related kinase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF8">
        <property type="entry name" value="NIMA-RELATED KINASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="14 23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="29"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="458"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="348"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00055453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00073378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00075647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="599" mass="68544" checksum="99B647AD737340ED" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GL42</accession>
    <name>A0A452GL42_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">2-aminomuconic semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="9">1.2.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aldehyde dehydrogenase family 8 member A1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the NAD-dependent oxidation of 2-aminomuconic semialdehyde of the kynurenine metabolic pathway in L-tryptophan degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-aminomuconate 6-semialdehyde + NAD(+) + H2O = (2Z,4E)-2-aminomuconate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14469"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:77634"/>
            <dbReference type="ChEBI" id="CHEBI:77859"/>
            <dbReference type="EC" id="1.2.1.32"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid degradation; L-kynurenine degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.32" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452GL42"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002509"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002870.1">
        <property type="protein sequence ID" value="ENSGAGP00000002509.1"/>
        <property type="gene ID" value="ENSGAGG00000002025.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047102">
        <property type="term" value="F:aminomuconate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001758">
        <property type="term" value="F:retinal dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042573">
        <property type="term" value="P:retinoic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07093">
        <property type="entry name" value="ALDH_F8_HMSADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000001">
        <property type="entry name" value="Aldehyde dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000021">
        <property type="entry name" value="Aldehyde dehydrogenase family 8 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720:SF2">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="14">
        <location>
            <begin position="23"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="487" mass="53653" checksum="E50EB03AD1BF163A" modified="2019-05-08" version="1">MAAPKTLLVLENFINGKFVPCSSYIDSYDPSTGDVYCRVPDSGKEEVEIAVTAARDAFLSWSSKSLQERSQIMNKLADLIEHDLEAFAQAESKDQGKTITFARTVDIPRAAYNFRFFASSILHHTTECTQMDYMDCMHYTVRTPVGIAGLISPWNLPLHLLTWKIAPAIASGNTVIAKPSEMTSVTAWMMCKLLGRAGIPPGVVNIVFGTGPKAGEAIVSHPEVPLISFTGSTLTAQHIKEKSAPHWKKLSLELGGKNPAIIFEDANLDQCIPTTVRSSFANQGEICLCTSRIFVQRSIYNEFLKRFVEETRKWKVGSPSDPTVSMGALISKEHLEKVKNYVKKARAEGARILCGEGVDSLALPTGNQNGYFMLPTVIAEVKDESCCMQEEIFGPLTCVVLFDTEEEVIKRANGVKYGLAATIWSSNVGRVHRVAKRLQAGLVWTNCWLVRELNLPFGGMKASGIGREGAKASYEFFTEVKTITIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLJ6</accession>
    <name>A0A452GLJ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GLJ6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002652"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003037.1">
        <property type="protein sequence ID" value="ENSGAGP00000002652.1"/>
        <property type="gene ID" value="ENSGAGG00000002132.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006418">
        <property type="term" value="P:tRNA aminoacylation for protein translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1290:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1290">
        <property type="entry name" value="Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007639">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042558">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042559">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04558">
        <property type="entry name" value="tRNA_synt_1c_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="6">
        <location>
            <begin position="7"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="202" mass="21620" checksum="89D9C68ABF8FE82A" modified="2019-05-08" version="1">MAAGPDALSLFTAIGLSEPKARETLKNEALSALLREAVTQAQGILGPNVDKATGTLLYNVASRLKDQKRLCFLVGCITSKKIVTDLQLSAALEYVRSHPLDPIDTADFEQECGVGVVVTPEQIEEAVEAAVNKHRAELLSERYHFNMGLLMGESAAQAGQGVGLRGGDLCAVLHGVDQEVPPPQCVPWLCSLPPCWAHSLCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLU9</accession>
    <name>A0A452GLU9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Zinc finger homeobox protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">AT motif-binding factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">AT-binding transcription factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Alpha-fetoprotein enhancer-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Zinc finger homeodomain protein 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 25 26">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000002617"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002991.1">
        <property type="protein sequence ID" value="ENSGAGP00000002617.1"/>
        <property type="gene ID" value="ENSGAGG00000002102.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045664">
        <property type="term" value="P:regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000082">
        <property type="entry name" value="Putative zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000317">
        <property type="entry name" value="zinc finger homeobox protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000378">
        <property type="entry name" value="zinc finger homeobox protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000429">
        <property type="entry name" value="Zinc finger homeobox protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000058">
        <property type="entry name" value="zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000064">
        <property type="entry name" value="Zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000096">
        <property type="entry name" value="Zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000081">
        <property type="entry name" value="Zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051968">
        <property type="entry name" value="ZnFinger_Homeobox_TR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45891">
        <property type="entry name" value="ZINC FINGER HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45891:SF4">
        <property type="entry name" value="ZINC FINGER HOMEOBOX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24056">
        <property type="entry name" value="zf-C2H2_ZFHX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 25 26" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="15 25 26" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="18 25 26" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 24" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="29">
        <location>
            <begin position="724"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="29">
        <location>
            <begin position="1087"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="29">
        <location>
            <begin position="1368"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="29">
        <location>
            <begin position="1409"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="29">
        <location>
            <begin position="1553"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="29">
        <location>
            <begin position="1604"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="29">
        <location>
            <begin position="1977"/>
            <end position="2005"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="28">
        <location>
            <begin position="2137"/>
            <end position="2197"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="28">
        <location>
            <begin position="2234"/>
            <end position="2294"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="29">
        <location>
            <begin position="2322"/>
            <end position="2351"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="29">
        <location>
            <begin position="2523"/>
            <end position="2545"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="28">
        <location>
            <begin position="2632"/>
            <end position="2692"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="29">
        <location>
            <begin position="2704"/>
            <end position="2734"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="28">
        <location>
            <begin position="2937"/>
            <end position="2997"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="25">
        <location>
            <begin position="2139"/>
            <end position="2198"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="25">
        <location>
            <begin position="2236"/>
            <end position="2295"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="25">
        <location>
            <begin position="2634"/>
            <end position="2693"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="25">
        <location>
            <begin position="2939"/>
            <end position="2998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="415"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="592"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1149"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1308"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1500"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1638"/>
            <end position="1717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1846"/>
            <end position="1931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="2024"/>
            <end position="2076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="2217"/>
            <end position="2236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="2370"/>
            <end position="2391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="2408"/>
            <end position="2501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="2612"/>
            <end position="2640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="2905"/>
            <end position="2940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="3129"/>
            <end position="3227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="3336"/>
            <end position="3404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="3507"/>
            <end position="3645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="27">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="429"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="27">
        <location>
            <begin position="457"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="524"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="1167"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="1197"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="1315"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="1512"/>
            <end position="1523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="1641"/>
            <end position="1682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="1846"/>
            <end position="1859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="1867"/>
            <end position="1894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="27">
        <location>
            <begin position="2028"/>
            <end position="2052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="2431"/>
            <end position="2454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="2467"/>
            <end position="2476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="27">
        <location>
            <begin position="2477"/>
            <end position="2491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="2492"/>
            <end position="2501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="2914"/>
            <end position="2930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="3146"/>
            <end position="3163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="3165"/>
            <end position="3178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="3190"/>
            <end position="3222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="3336"/>
            <end position="3350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="3365"/>
            <end position="3380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="3393"/>
            <end position="3403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="3524"/>
            <end position="3551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="3576"/>
            <end position="3596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00067473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00076607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00077229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00077962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00083807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="3645" mass="399862" checksum="AA9AEE55B8C742A4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNQ0</accession>
    <name>A0A452GNQ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GNQ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003509"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004047.1">
        <property type="protein sequence ID" value="ENSGAGP00000003509.1"/>
        <property type="gene ID" value="ENSGAGG00000002832.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF142">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="6"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="82" mass="9373" checksum="8AC50824B5DD5450" modified="2019-05-08" version="1">LLKTFTSSSEVALYFTREEGALLDLTQRALYRDVMQENYESVVLLGFPVSKPDVISKLERGEEPWVPDLQGRCHSWLPTCPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNU6</accession>
    <name>A0A452GNU6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tubulin/FtsZ GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GNU6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003574"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004120.1">
        <property type="protein sequence ID" value="ENSGAGP00000003574.1"/>
        <property type="gene ID" value="ENSGAGG00000002882.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="10566" checksum="F00CA00F30E7E50F" modified="2019-05-08" version="1">MSIVTVQLGQCGNQIGYEVFNAICNDFHSTHGLCSKKENESYQEACKERFFSEEKTGGTIFKDFFFSYHTEPVRNIQTCFCSFQISVYYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GR92</accession>
    <name>A0A452GR92_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the BCOR family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000004225"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004956.1">
        <property type="protein sequence ID" value="ENSGAGP00000004225.1"/>
        <property type="gene ID" value="ENSGAGG00000003432.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14261">
        <property type="entry name" value="PUFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000032">
        <property type="entry name" value="BCL-6 corepressor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.40:FF:000001">
        <property type="entry name" value="BCL-6 corepressor isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.40">
        <property type="entry name" value="BCL-6 corepressor, PCGF1 binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031628">
        <property type="entry name" value="BCOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047144">
        <property type="entry name" value="BCOR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032365">
        <property type="entry name" value="PUFD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038227">
        <property type="entry name" value="PUFD_som_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24117">
        <property type="entry name" value="AGAP007537-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24117:SF8">
        <property type="entry name" value="BCL-6 COREPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15808">
        <property type="entry name" value="BCOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16553">
        <property type="entry name" value="PUFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BCL-6 corepressor non-ankyrin-repeat" evidence="14">
        <location>
            <begin position="1204"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="1507"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="1540"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="domain" description="BCL-6 corepressor PCGF1 binding" evidence="15">
        <location>
            <begin position="1643"/>
            <end position="1755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="382"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="601"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="731"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1071"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1221"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="558"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="732"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1084"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1168"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1252"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1300"/>
            <end position="1316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF15808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1777" mass="197565" checksum="3140F7A50B838E56" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRB1</accession>
    <name>A0A452GRB1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear receptor coactivator 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GRB1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004246"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004985.1">
        <property type="protein sequence ID" value="ENSGAGP00000004246.1"/>
        <property type="gene ID" value="ENSGAGG00000003525.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052600">
        <property type="entry name" value="Nuc_rcpt_coact/corep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23295">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23295:SF5">
        <property type="entry name" value="SI:CH211-216L23.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="55349" checksum="266B0749FFAC2136" modified="2019-05-08" version="1">MSSWVKVTRGQVQKPIVDKRHRPGWIPRLLDINPYRPYQKSPYSPQDGYSKDFEDLNSFEELFGQQYDYSTGNEDYYERYQYSPKGPSLHETPGIPGRQEAAVAAQQPGSSRRQDPEHESSAVEVGPTAPCSREKWRTARAASVVPPAGVSPLPVVDDDYTRDDRREKLYLQFYQQIQKEYDKERPSDCIIVAISKEQTEYATVIGHRLQDHGLVVEMIYLTSELGLTRALQEVKNDGSPFCILVEQSNVALSSCTVIMLHESIKIHRNMPMEDALALVVKAFGKIFAEREQQERAEISHKAADLADDYLERELCGSYSVPLGVRHLLFLLSEGKYLYREELNSISDYLKTRKDELEALVEQTDPSTEVEDTLYQANLMPSTSTATHTLSKPPPLLPTPNRNILLGQSPLPPAKPALLGDRPSGALLPTPGMCGAKGKPPPLLAAPAKRPGLLGYSPHQPAKRPLLGEKPGLLPTPAVHVKPVQHQRAAKPKPLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRC7</accession>
    <name>A0A452GRC7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neuropeptide FF receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">G-protein coupled receptor 147</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">RFamide-related peptide receptor OT7T022</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GRC7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004518"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005294.1">
        <property type="protein sequence ID" value="ENSGAGP00000004518.1"/>
        <property type="gene ID" value="ENSGAGG00000003700.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000153">
        <property type="entry name" value="Neuropeptide FF receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005395">
        <property type="entry name" value="NPFF_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF82">
        <property type="entry name" value="NEUROPEPTIDE FF RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01570">
        <property type="entry name" value="NPFFRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="155"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="56"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="431" mass="48677" checksum="C873A0ACBBB297BF" modified="2019-05-08" version="1">MDGPEMESEGRINKTISSLMSHKRNLTYLPYYQHSPPVAAIYIISYLFIFALCMVGNSLVCFIVLKNSRMRTVTNLFILNLAISDLLVGIFCVPTTLVDNLITGWPFSNGICKMSGLVQGMSVSSSVFTLVAIAVDRFRCIIYPFKSKLSLLKALNTIAVIWVLAITIMCPSAVMLTVVQIEDHYMVYNDNQSVAYPLYSCYEAWPNSEMRKVYTTVLFAHIYMVPLTLIMVMYGRIGVKLYRSSGPVSKHRGENHECCSAAVSRRKVKVIKMLILVALLFMLSWLPLWTLMLFTDYTKLDDDQIDLLTGYIFPSAHWLAFSNSSVNPIIYGYFNENFKRGFQAAFKFQVCSEEIGRHEVYSEKVMGSTSGFSARSKIFTDKESSDSVCLKNLHAKAPCLVQNGQAQKQGLHLQDIDKITPLNKVCKAWDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRH9</accession>
    <name>A0A452GRH9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GRH9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004546"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005322.1">
        <property type="protein sequence ID" value="ENSGAGP00000004546.1"/>
        <property type="gene ID" value="ENSGAGG00000003752.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF133">
        <property type="entry name" value="RIKEN CDNA 1700020N01 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="2">
        <location>
            <begin position="8"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="70" mass="8364" checksum="959F6D4209A63116" modified="2019-05-08" version="1">MNHSFMSVTFEDVAMYFSPAEWALLGEEQRQLYRHVMWENYQILVSLDGNSITLSALILFMIYYTHHILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTB6</accession>
    <name>A0A452GTB6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000005276"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006141.1">
        <property type="protein sequence ID" value="ENSGAGP00000005276.1"/>
        <property type="gene ID" value="ENSGAGG00000004274.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000003">
        <property type="entry name" value="Putative rho GTPase-activating protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF103">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="20"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="226" mass="25206" checksum="4707831E1130426F" modified="2019-05-08" version="1">RPPLQSLQEKGLIRDQVFGCRLDALCQRENDTVPRFVRLCVEAVEKRGLDVDGIYRVSGNLAVIQKLRFAVDRGERLCLGGERGHLPXEGAVAPGRLSLADPEWDDVHVVTGALKLFFRELPEPLVPFALFDLPAYDERVQCLAELVQSLPPPNYATLRYILGHLPTSYADANRMTRQNIGIVFGPTLLRPEKELASMAVDMVHQNQAVELLLAEFQRIFPAPSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUE5</accession>
    <name>A0A452GUE5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the vacuolar ATPase subunit S1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GUE5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005680"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006625.1">
        <property type="protein sequence ID" value="ENSGAGP00000005680.1"/>
        <property type="gene ID" value="ENSGAGG00000004619.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033176">
        <property type="term" value="C:proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030641">
        <property type="term" value="P:regulation of cellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008388">
        <property type="entry name" value="Ac45_acc_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046756">
        <property type="entry name" value="VAS1/VOA1_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046755">
        <property type="entry name" value="VAS1_LD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12471:SF2">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12471">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20520">
        <property type="entry name" value="Ac45-VOA1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05827">
        <property type="entry name" value="VAS1_LD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="V-type proton ATPase subunit S1 luminal" evidence="7">
        <location>
            <begin position="78"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="V-type proton ATPase subunit S1/VOA1 transmembrane" evidence="8">
        <location>
            <begin position="228"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="278" mass="29742" checksum="140C72B5FC011876" modified="2019-05-08" version="1">MAPKEVLMSNDEIIGQVLSALKEEDVPYTALLTAGAPSRVLRDVSGVAPGRVGRQLLQKPTPAPAQHPPVRYPLTGEPQILFWAQNFSVVQGSKWVDLTPQTFGAEASVDVSGSFWSSNELVLKYANVLGSPLTATWRFPVSGRDWFQLNRLELATPSASPTVFNASQVTAPSNYSWRCAYLSSLPGFGAGLLPHGPGPSWGVLLQDVQLQGFNVPGLQFSYASDCAGFFAPGTWMGLLTALLLGAIFACGLHVLLALKTMDRFDDPKGATIAVPQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GV99</accession>
    <name>A0A452GV99_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GV99"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006053"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007052.1">
        <property type="protein sequence ID" value="ENSGAGP00000006053.1"/>
        <property type="gene ID" value="ENSGAGG00000004917.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06799">
        <property type="entry name" value="PDZ_MPP3-MPP4-MPP7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12039">
        <property type="entry name" value="SH3_MPP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000046">
        <property type="entry name" value="MAGUK p55 subfamily member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035604">
        <property type="entry name" value="MPP3_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="12">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="12">
        <location>
            <begin position="61"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="137"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="226"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="10">
        <location>
            <begin position="382"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="582" mass="66339" checksum="87CA20809BFAE6E4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVZ2</accession>
    <name>A0A452GVZ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">BRCA1-associated protein</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">BRAP2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Impedes mitogenic signal propagation</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">RING-type E3 ubiquitin transferase BRAP2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452GVZ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006221"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007233.1">
        <property type="protein sequence ID" value="ENSGAGP00000006221.1"/>
        <property type="gene ID" value="ENSGAGG00000005007.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16457">
        <property type="entry name" value="RING-H2_BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12718">
        <property type="entry name" value="RRM_BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000206">
        <property type="entry name" value="BRCA1-associated protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000159">
        <property type="entry name" value="BRCA1-associated protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011422">
        <property type="entry name" value="BRAP2/ETP1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034932">
        <property type="entry name" value="BRAP2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047243">
        <property type="entry name" value="RING-H2_BRAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007:SF7">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07576">
        <property type="entry name" value="BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="21">
        <location>
            <begin position="266"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="UBP-type" evidence="22">
        <location>
            <begin position="303"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="82"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="568"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="440"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="82"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="568"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="583"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="593" mass="67590" checksum="D41B2DB0FB34A3EB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWZ8</accession>
    <name>A0A452GWZ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GWZ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006652"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007729.1">
        <property type="protein sequence ID" value="ENSGAGP00000006652.1"/>
        <property type="gene ID" value="ENSGAGG00000005371.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000018">
        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF234">
        <property type="entry name" value="PROTOCADHERIN GAMMA-A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="688"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="27"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="134"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="243"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="356"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="453"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="579"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="870" mass="96195" checksum="EEE67022010A635A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYF7</accession>
    <name>A0A452GYF7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DnaJ homolog subfamily B member 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Endoplasmic reticulum DNA J domain-containing protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Co-chaperone for Hsp70 protein HSPA5/BiP that acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). J domain-containing co-chaperones stimulate the ATPase activity of Hsp70 proteins and are required for efficient substrate recognition by Hsp70 proteins. In the unstressed endoplasmic reticulum, interacts with the luminal region of ERN1/IRE1 and selectively recruits HSPA5/BiP: HSPA5/BiP disrupts the dimerization of the active ERN1/IRE1 luminal region, thereby inactivating ERN1/IRE1. Also involved in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Required for survival of B-cell progenitors and normal antibody production.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with HSPA5/BiP; interaction is direct. Interacts with ERN1/IRE1 (via the luminal region). Interacts with DERL1.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GYF7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007152"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008276.1">
        <property type="protein sequence ID" value="ENSGAGP00000007152.1"/>
        <property type="gene ID" value="ENSGAGG00000005749.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="6"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00041533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="59" mass="6843" checksum="2FBAAE471AE0E6B4" modified="2019-05-08" version="1">MVKETGYYDLLGVKPGATLDEIKRAYRRLALKYHPDKNPSEGERIRLRFCSWLQDGSVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYK8</accession>
    <name>A0A452GYK8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GYK8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007139"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008262.1">
        <property type="protein sequence ID" value="ENSGAGP00000007139.1"/>
        <property type="gene ID" value="ENSGAGG00000005738.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048788">
        <property type="term" value="C:cytoskeleton of presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050806">
        <property type="term" value="P:positive regulation of synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048167">
        <property type="term" value="P:regulation of synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000300">
        <property type="term" value="P:regulation of synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04028">
        <property type="entry name" value="C2B_RIM1alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000001">
        <property type="entry name" value="Regulating synaptic membrane exocytosis 3, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039032">
        <property type="entry name" value="Rim-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12157:SF26">
        <property type="entry name" value="REGULATING SYNAPTIC MEMBRANE EXOCYTOSIS 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12157">
        <property type="entry name" value="REGULATING SYNAPTIC MEMBRANE EXOCYTOSIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="62"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="216" mass="23528" checksum="C403031273CA43F4" modified="2019-05-08" version="1">MPSRTVRQASHESIEDSMNSYGSEGNLNFSGVHLASAGQFSDFLGSMGPAQFVGRQTLATTSMGDVEIGLQERNGQLEVDIIQARGLTPKPGSKTLPAAYIKAYLLENGVCIAKKKTKVARKSLDPLYNQVLLFAESPQGKVLQVIVWGNYGRMERKHFMGVARVLLEELDLSTLAIGWYKLFPTSSMVDPTTGPLLRQSSQLSLESTVGPCCDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZI0</accession>
    <name>A0A452GZI0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kelch-like protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the KLHL3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZI0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007473"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008629.1">
        <property type="protein sequence ID" value="ENSGAGP00000007473.1"/>
        <property type="gene ID" value="ENSGAGG00000005975.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050801">
        <property type="term" value="P:monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070294">
        <property type="term" value="P:renal sodium ion absorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18513">
        <property type="entry name" value="BACK_KLHL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18339">
        <property type="entry name" value="BTB_POZ_KLHL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000001">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030578">
        <property type="entry name" value="KLHL3_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF179">
        <property type="entry name" value="KELCH-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="13">
        <location>
            <begin position="74"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="479" mass="53853" checksum="05994EF6BC9051B0" modified="2019-05-08" version="1">MSCITSLWLEQLFQSYASKSFTPPLSPAFIKQSSQSPDEAEDKEKDQRTVTVNPSHMGKAFKVMNELRSKRLLCDVVIVAEAIEMEAHRVVLAACSPYFCAMFTGDMTERKAKKIEIKDVDGQTLSKLIDYIYTAEIEVTEENVQVLLPAASLLQLMDVRQNCCDFLQSQLHPTNCLGIRAFADVHTCTELLQQANAYAEQHFPEVMLGEEFLNLSLDQVCSLISSDKLTVSSEEKVFEAVISWINYEKESRLEHMAELMEHVRLPLLPRDYLVQTVEEEALIKNNNTCKDFLIEAMKYHLLPLDQRQLIKNPRTKPRTPVSLPKVMIVVGGQAPKAIRSVECYDFEEDRWEQVAELPSRRCRAGVVFMAGNIYAVGGFNGSLRVRTVDVYDGVKDQWTSIASMQERRSTLGAAVLNELLYAVGGFDGSTGLASVEAYSYKTNEWFFVAPMNTRRSSVGVGVVEGKNNSSHFSHLNTAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H038</accession>
    <name>A0A452H038_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H038"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008992.1">
        <property type="protein sequence ID" value="ENSGAGP00000007801.1"/>
        <property type="gene ID" value="ENSGAGG00000006213.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051375">
        <property type="entry name" value="Tuftelin_GRINL1A/MYZAP/CCD68"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23171:SF3">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23171">
        <property type="entry name" value="GDOWN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="149"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="236"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="320" mass="37100" checksum="4BF46FE5623B496B" modified="2019-05-08" version="1">MATTVRLTEHVTRDDQGMEGNYVLHGSSCAQITEETEYIKKIRSTLAKIQNQQPFRVPRNELEAEKDTLNSSYNLTVEKLKETDQQLLLVNRENEILKIKLDATRDAGAQSLRHASESLYENYCNRSEELKKKHGDDKQLMQACKLEQEQKLKQNLENVNCVTERLEEKYSQIADMEKRVQRMEEEKKTLIEKKQSFEKKLLLNSEDTKSCPDLQVEIFTVQEQICHLQHVIQTQHQSLRSAIQEIEELNNGLQNQDKKIEAMKEKINMLEAKNKELKYKVEFWSGQPKIKVSKGVSAISSKTDGVSPYSMLARLRKQNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H113</accession>
    <name>A0A452H113_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the SFT2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H113"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008201"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009442.1">
        <property type="protein sequence ID" value="ENSGAGP00000008201.1"/>
        <property type="gene ID" value="ENSGAGG00000006507.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF24">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="137" mass="15326" checksum="326361AA8FD70241" modified="2019-05-08" version="1">MEKLRRVLSGHEDEEQGLTAQVLDASTLSFGTRVKWFAICFATGIFCSILGTAFLWLPSGLKLFAVFYTIGNISALASTCFLMGPVKQLKKMFEPTRLIATIVMLLCLICTLCAVFWWGKRGLALLFCILQFLAMTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1S0</accession>
    <name>A0A452H1S0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H1S0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008331"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009590.1">
        <property type="protein sequence ID" value="ENSGAGP00000008331.1"/>
        <property type="gene ID" value="ENSGAGG00000006618.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000031">
        <property type="entry name" value="Ankyrin repeat, family A (RFXANK-like), 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124:SF3">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY A PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="247"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="313" mass="34298" checksum="3B4232ABF8E4F89E" modified="2019-05-08" version="1">MASSTNLDVGAQIIVEECTSSYSLSHMPDIKVEHQLDSTTEDGPAQSVAMGMKFILPNRFDMNVCSRFVKSLNEEDSKNIQDQVNSDLEVASVLFKAECNIHTSPSPGIQVRHVYTPSTTKHFSPIKQSTTLTNKHRGNEVSTTPLLVNSLSVHQLAAQGEMLYLATRIEQENVINHKDEEGFTPLMWAAAHGQIAVVEFLLQNGADAQILGKGRESALSLACSKGYTDIVKMLLDCGVDVNEYDWNGGTPLLYAVHGNHVKCVKILLENGADPTIETDSGYNSMDLAVALGHRSGKDLEPFRFGCFYCSLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H200</accession>
    <name>A0A452H200_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H200"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008525"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009812.1">
        <property type="protein sequence ID" value="ENSGAGP00000008525.1"/>
        <property type="gene ID" value="ENSGAGG00000006770.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000010">
        <property type="entry name" value="Zinc finger protein 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001442">
        <property type="entry name" value="zinc finger protein 696"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000933">
        <property type="entry name" value="zinc finger protein 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="19"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="129"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="195" mass="21897" checksum="0B0D786AFB044A0E" modified="2019-05-08" version="1">IQSSDLLRHLRTHTGERPYKCPECGRGFGDSSTLSDLIRHQRTHTGERPYKCPDCSKGFSDSSTLIKHQRTHTGERPYKCMCGKRFGLSSNLLQHQRTHTGERPFACAQCGKRFGDSSTLSKHRRTHVGQCSFASAQCTEGLGDSAMLLQHPHTHQHEQPYQCIQCSKAFSQSSSLAQHQHTHKDWPLCAIAPCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2N0</accession>
    <name>A0A452H2N0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H2N0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008715"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010012.1">
        <property type="protein sequence ID" value="ENSGAGP00000008715.1"/>
        <property type="gene ID" value="ENSGAGG00000006891.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039341">
        <property type="entry name" value="CFAP99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34649">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34649:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="362"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="493"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="661" mass="77346" checksum="96B187D2E6785EBE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2X8</accession>
    <name>A0A452H2X8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H2X8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008831"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010156.1">
        <property type="protein sequence ID" value="ENSGAGP00000008831.1"/>
        <property type="gene ID" value="ENSGAGG00000006988.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="219" mass="24106" checksum="81E2C70E4BA850A8" modified="2019-05-08" version="1">MLSGAPAELSDLQSSCHPYCIDTTSLLAWLQELPAVGDAVAEAPSAGQAHVGPVPTAAQVRRLGFWLKVFPQSVQLGFQLKPFPHTVQAKGRSPLLPHWVHWNGRSPVWVRRCVTSVTFCRKHFPHSGQAYGRSPPGTRWGSCRPKLFPHTSQRWGLSPQWLSRCFSSWLCKLKLRLHSGHVQAVCPTCASCGAGRDSRGLRPFPRSGSGYGCHPAWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3C8</accession>
    <name>A0A452H3C8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM132 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3C8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010312.1">
        <property type="protein sequence ID" value="ENSGAGP00000008978.1"/>
        <property type="gene ID" value="ENSGAGG00000007079.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000010317.1">
        <property type="protein sequence ID" value="ENSGAGP00000008983.1"/>
        <property type="gene ID" value="ENSGAGG00000007079.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031437">
        <property type="entry name" value="Ig_TMEM132_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055423">
        <property type="entry name" value="Ig_TMEM132_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055424">
        <property type="entry name" value="Ig_TMEM132_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026307">
        <property type="entry name" value="TMEM132"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031436">
        <property type="entry name" value="TMEM132_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13388">
        <property type="entry name" value="DETONATOR, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13388:SF12">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 132B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16070">
        <property type="entry name" value="Ig_TMEM132_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23486">
        <property type="entry name" value="Ig_TMEM132_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23487">
        <property type="entry name" value="Ig_TMEM132_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15706">
        <property type="entry name" value="TMEM132_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="482"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein family 132 fourth" evidence="9">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 fifth" evidence="10">
        <location>
            <begin position="100"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 sixth" evidence="11">
        <location>
            <begin position="237"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 C-terminal" evidence="8">
        <location>
            <begin position="454"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="417"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="445"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF15706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="661" mass="73949" checksum="B1FA00CD5303296C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4F3</accession>
    <name>A0A452H4F3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytotoxic granule associated RNA binding protein TIA1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">RNA-binding protein TIA-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">T-cell-restricted intracellular antigen-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Homooligomer; homooligomerization is induced by Zn(2+). Interacts with FASTK; the interactions leads to its phosphorylation. Interacts (via RRM1 and the C-terminal glutamine-rich (Q) sequence) with SNRPC/U1-C (via N-terminus); thereby facilitating spliceosomal U1 snRNP recruitment to 5' splice sites.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H4F3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009515"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010933.1">
        <property type="protein sequence ID" value="ENSGAGP00000009515.1"/>
        <property type="gene ID" value="ENSGAGG00000007529.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097165">
        <property type="term" value="C:nuclear stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12615">
        <property type="entry name" value="RRM1_TIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12618">
        <property type="entry name" value="RRM2_TIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12620">
        <property type="entry name" value="RRM3_TIAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000951">
        <property type="entry name" value="nucleolysin TIA-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000038">
        <property type="entry name" value="Nucleolysin tiar isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000045">
        <property type="entry name" value="Nucleolysin tiar isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034827">
        <property type="entry name" value="TIA-1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034830">
        <property type="entry name" value="TIA1_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034496">
        <property type="entry name" value="TIAR_RRM3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 15" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="7"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="106"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="214"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="384" mass="42640" checksum="C6D8D595C75258CB" modified="2019-05-08" version="1">MEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCFVEFYEHRHAAAALAAMNGRKIMGKEVKVNWATTPSSQKKDTSSSTVVSTLRSQDHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDDVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFIRFNSHESAAHAIVSVNGTTIEGHVVKCYWGKETPDMINPIQQNQIGYPQAYGQWGQWYGNAQQIGQYMPNGWQVPAYGMYGQAWNQQGFNQTQSSAAWMGANYGVPPPQGQNGSVLTNQTGYRMAGFETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H698</accession>
    <name>A0A452H698_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ODAD1 central coiled coil region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H698"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010220"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011728.1">
        <property type="protein sequence ID" value="ENSGAGP00000010220.1"/>
        <property type="gene ID" value="ENSGAGG00000008008.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051876">
        <property type="entry name" value="ODA-DC/CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049258">
        <property type="entry name" value="ODAD1_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21694">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21694:SF18">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21773">
        <property type="entry name" value="ODAD1_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ODAD1 central coiled coil region" evidence="3">
        <location>
            <begin position="71"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="260"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="466" mass="54374" checksum="61F1F24640387700" modified="2019-05-08" version="1">MKRLNMMLDDRNCMELKFLLQTKEEYDSLIRATKALIAELDDKVMEMEKKIKTQKLVVAKVKQVNDSKWLQKQIQMLENRLNHVTVHFDTILTTNATLREEIENLRCQKAIFDNFYSKLHKKLDQQKKTMDNAIEQSSQAYEQRVEALARISAMKERRCKDIAQYNVEFRELERVFDHETKLKAFMLIKLVDRSDFEEQAKREEALKASKRARKSKGESFESYEVAYMRLLKLSEDGDIGQLVADFIEKEEKNFAYFSYVTELNNEMERLQKKTQDIQNEILHLKSQQKFADDKSNTSLKEMEEKLKKTTEEANLYEHTCKESSKVLDQLKSAVDFLFKQIHCEDTKIMEQLGESGEITDLNLMQYFGIIEKKTNDLLLIESFLRYKEVEGEQDSLPLLNPFLGGTGLLKGTDAAKLPPSSPTIEHMAENIEDCKYSHLLTQPHLHPNEKSSGISLDKEKKRGIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6R7</accession>
    <name>A0A452H6R7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Amine oxidase</fullName>
            <ecNumber evidence="12">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="12">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="11">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="11 12">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452H6R7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010410"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011949.1">
        <property type="protein sequence ID" value="ENSGAGP00000010410.1"/>
        <property type="gene ID" value="ENSGAGG00000008152.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.20:FF:000015">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000001">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000003">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049947">
        <property type="entry name" value="Cu_Am_Ox_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049948">
        <property type="entry name" value="Cu_Am_ox_TPQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015800">
        <property type="entry name" value="Cu_amine_oxidase_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015802">
        <property type="entry name" value="Cu_amine_oxidase_N3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF4">
        <property type="entry name" value="RETINA-SPECIFIC COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02727">
        <property type="entry name" value="Cu_amine_oxidN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02728">
        <property type="entry name" value="Cu_amine_oxidN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00766">
        <property type="entry name" value="CUDAOXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01164">
        <property type="entry name" value="COPPER_AMINE_OXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01165">
        <property type="entry name" value="COPPER_AMINE_OXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 12" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 12" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase N2-terminal" evidence="14">
        <location>
            <begin position="76"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N3-terminal" evidence="15">
        <location>
            <begin position="162"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="13">
        <location>
            <begin position="305"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="10">
        <location>
            <position position="461"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="11">
        <location>
            <position position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="756" mass="85107" checksum="66C1A32C2BAA0A8D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8X0</accession>
    <name>A0A452H8X0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbonic anhydrase-related protein 10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Does not have a catalytic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H8X0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011244"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012877.1">
        <property type="protein sequence ID" value="ENSGAGP00000011244.1"/>
        <property type="gene ID" value="ENSGAGG00000008695.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03121">
        <property type="entry name" value="alpha_CARP_X_XI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000002">
        <property type="entry name" value="Carbonic anhydrase-related protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041878">
        <property type="entry name" value="Alpha_CARP_X/XI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF91">
        <property type="entry name" value="CARBONIC ANHYDRASE-RELATED PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018993621" description="Carbonic anhydrase-related protein 10" evidence="4">
        <location>
            <begin position="22"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="5">
        <location>
            <begin position="31"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="332" mass="38046" checksum="49ECDCD3159C78D2" modified="2019-05-08" version="1">MEIVWEVLFLLQANFIVCISAQQNSPKIHEGWWAYKEVVQGSFVPVPSFWGLVNSAWNLCAVGKRQSPVNIETSHMIFDPFLTPLRINTGGRKVSGTMYNTGRHVSLRLDKEHLVNISGGPMTYSHRLEEIRLHFGSEDSQGSEHLLNGQAFSGEVQLIHYNHELYTNVTEAAKSPNGLVVVSIFMKVSESSNPFLNRMLNRDTITRITYKNDAYLLQGLNIEELYPETSSFITYDGSMTIPPCYETASWIIMNKPVYITRMQMHSLRLLSQNQPSQIFLSMSDNFRPVQPLNNRCIRTNINFSLQGKDCPNNRAQKLQYRGRFYTRQSPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAW9</accession>
    <name>A0A452HAW9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Neuronal growth regulator 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the immunoglobulin superfamily. IgLON family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HAW9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011892"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013620.1">
        <property type="protein sequence ID" value="ENSGAGP00000011892.1"/>
        <property type="gene ID" value="ENSGAGG00000008915.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000013">
        <property type="entry name" value="cell adhesion molecule 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000305">
        <property type="entry name" value="neurotrimin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050876">
        <property type="entry name" value="IgLON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42757">
        <property type="entry name" value="IGLON FAMILY OF IMMUNOGLOBULIN SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42757:SF6">
        <property type="entry name" value="NEURONAL GROWTH REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="132"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="212"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="358" mass="39486" checksum="57591A8A216A7BC8" modified="2019-05-08" version="1">MLGVVHNAPLSCSSHDNQLLPWFLSWLADGREFVSAATQSVAAQKALTPSQLCYLEDGASKGAWLNRSSIIFAGGDKWSVDPRVSIATANKREYSLQIQDVDVMDDGPYTCSVQTQHTPRTMQVHLTVQVSPKISRISTDTVVNEGSNVTLICLPSISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSAENDASVPDVKKVKVTVNFAPTIQELKSSGVMLGGNGLIRCESAGVPAPVFEWYKGERKLINGKHGITIKNYSTRSLLTVTNVTEEHFGNYTCVAANKLGVTNASLPLNRVGKLPNVSEQLLIGCFYISCIFFICRFIYRRNMTSQLSRASTHFLGEEWSYLCCILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCR2</accession>
    <name>A0A452HCR2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fibrinogen-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3. Responsible for LAG3 T-cell inhibitory function. Binds LAG3 independently from MHC class II (MHC-II). Secreted by, and promotes growth of, hepatocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. Interacts (via the Fibrinogen C-terminal domain) with LAG3 (via Ig-like domains 1 and 2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HCR2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012578"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014418.1">
        <property type="protein sequence ID" value="ENSGAGP00000012578.1"/>
        <property type="gene ID" value="ENSGAGG00000009660.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005577">
        <property type="term" value="C:fibrinogen complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072377">
        <property type="term" value="P:blood coagulation, common pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042730">
        <property type="term" value="P:fibrinolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF8">
        <property type="entry name" value="FIBRINOGEN LIKE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019388867" description="Fibrinogen-like protein 1" evidence="11">
        <location>
            <begin position="21"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="12">
        <location>
            <begin position="75"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="313" mass="36485" checksum="89BC6F33A2B827E4" modified="2019-05-08" version="1">MKILTLFNFILANCLIMATGNSTLQSCFQEQLRLKAQVRLLEYRVKQQQVKIVRILQEKEIQYRDSGDENSVIDLGEKRQYADCAEIYNDGHKRSGFYKMKPLQSPSEFLAYCDMSEGGGWTVFQRRSDGSQKFDRVWADYEQGFGNFVLRNGEYWLGNKNLHYLTNQGNYTLRIDLSDFEGERRYAQYKSFRVADEESSYQLSCGEYSGTAGDSLTGGFHPEVKWWADHRGMKFSTQDRDNDNYEGNCAEEDTAGWWFNRCHTSNLNGVYYKGPYTAKTDNGIVWYTWHGWWYSLKSVVMKVRPADFEPNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HE43</accession>
    <name>A0A452HE43_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="20">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 25">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 25 20">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPP phosphatase family. PP-1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A452HE43"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013089"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014992.1">
        <property type="protein sequence ID" value="ENSGAGP00000013089.1"/>
        <property type="gene ID" value="ENSGAGG00000010039.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF377">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE PP1-ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="11 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="22">
        <location>
            <begin position="121"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="330" mass="37425" checksum="A07EF5DA721065D7" modified="2019-05-08" version="1">MSEGEKLNLDSIIGRLLEVQGSRPGKNVQLTESEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCGHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGSEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKNKGKYGQFSGLNPGGRPVTPPRNSAKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HF48</accession>
    <name>A0A452HF48_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Helicase, lymphoid specific</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HF48"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013462"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015407.1">
        <property type="protein sequence ID" value="ENSGAGP00000013462.1"/>
        <property type="gene ID" value="ENSGAGG00000010287.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006346">
        <property type="term" value="P:DNA methylation-dependent constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046651">
        <property type="term" value="P:lymphocyte proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47161">
        <property type="entry name" value="LYMPHOID-SPECIFIC HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47161:SF1">
        <property type="entry name" value="LYMPHOID-SPECIFIC HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="11064" checksum="A2BE8EFBBDE496A3" modified="2019-05-08" version="1">MPARNTPSSGGHLGAEMGEQAEAAVITPSMLEEEEQLEAAGMEKEREMLEKARMSWDRDSSEMRYKRLQHLLEKSNIYSKFLLTKMEQQQLEVDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFB6</accession>
    <name>A0A452HFB6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">T-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFB6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013495"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015449.1">
        <property type="protein sequence ID" value="ENSGAGP00000013495.1"/>
        <property type="gene ID" value="ENSGAGG00000010312.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20191">
        <property type="entry name" value="T-box_TBX15_18_22-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000001">
        <property type="entry name" value="T-box transcription factor TBX18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF98">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR TBX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="8">
        <location>
            <begin position="116"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="42"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="337"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="570"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="601" mass="65758" checksum="8B2E7F709AEB47E8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFV8</accession>
    <name>A0A452HFV8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HFV8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013721"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015715.1">
        <property type="protein sequence ID" value="ENSGAGP00000013721.1"/>
        <property type="gene ID" value="ENSGAGG00000010460.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="280" mass="28955" checksum="FC882F894BA4D292" modified="2019-05-08" version="1">MLLVQSLYVLEEGVVPHGQQGAWTGREPRSPGSQSSPHPRGGREPRSPGSQSSPHPRGGREPRSPGSQSSPHPCGGREPRSPGSQSSPHPCGGREPRSPGSQSSPHPCGGREPRSPGSQSSPHPCGGREPRSPGSQSSPHPCGGREPRSPGSQSSPHPCGGREPRKPRSPGSQSSPHPCGGREPRSPGSQSSPHPCGGREPRSPGSQSSPHPCGGREPRSPGSQSSPHPRGGREPRSPGSQSSPHPCGGREPRSPGSQSSPHPRGRREPRSPGSQSSPHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGP8</accession>
    <name>A0A452HGP8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Smaug homolog 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Sterile alpha motif domain-containing protein 4A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Synapse</location>
            <location evidence="10">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMAUG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGP8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014039"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016067.1">
        <property type="protein sequence ID" value="ENSGAGP00000014039.1"/>
        <property type="gene ID" value="ENSGAGG00000010681.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030371">
        <property type="term" value="F:translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09557">
        <property type="entry name" value="SAM_Smaug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000013">
        <property type="entry name" value="Protein Smaug homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.170:FF:000002">
        <property type="entry name" value="Protein Smaug homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.170">
        <property type="entry name" value="Smaug, PHAT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037093">
        <property type="entry name" value="PHAT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058599">
        <property type="entry name" value="PHAT_Smg/ZCCHC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050897">
        <property type="entry name" value="SMAUG/VTS1_RNA-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037634">
        <property type="entry name" value="Smaug_SAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515:SF8">
        <property type="entry name" value="PROTEIN SMAUG HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26034">
        <property type="entry name" value="PHAT_SMAUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="6" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SAM" evidence="14">
        <location>
            <begin position="320"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="278"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="422"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="544"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="427"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="689" mass="76219" checksum="9ADE51963AC29C29" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHJ4</accession>
    <name>A0A452HHJ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ZP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HHJ4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016387.1">
        <property type="protein sequence ID" value="ENSGAGP00000014321.1"/>
        <property type="gene ID" value="ENSGAGG00000010889.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035803">
        <property type="term" value="P:egg coat formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000002">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF3">
        <property type="entry name" value="SI:CH211-14A17.6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZP" evidence="2">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="246" mass="26743" checksum="6CD1A99C07AC00D4" modified="2019-05-08" version="1">MYVSSVTGHLSASGVLTFTVTLMNVDFSAPSDSKVIFLGSQIHMEFSVQSSFHQPMQIFVDECTATPTPELSKSLRNYSIIANHGCLVDGKVANSQFLPRRTPEAIQLSLQAFEFVGVDSDIYLHCQVLVWDPKVLMDPTRKACSFHRDTNRWELLDNPSLSSGCSCCDSLCQATASRHKRDLKEPGAGLVHAVVVGPLKVQKPAAKTGSYEWDSNSSPAAQSVTGNYPSCGLKLCSIATRQLLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHS6</accession>
    <name>A0A452HHS6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HHS6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014449"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016528.1">
        <property type="protein sequence ID" value="ENSGAGP00000014449.1"/>
        <property type="gene ID" value="ENSGAGG00000010983.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120025">
        <property type="term" value="C:plasma membrane bounded cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001136">
        <property type="term" value="P:negative regulation of endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04404">
        <property type="entry name" value="RhoGAP-p50rhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000024">
        <property type="entry name" value="Rho GTPase-activating protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000007">
        <property type="entry name" value="rho GTPase-activating protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049592">
        <property type="entry name" value="ARHGAP1_RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF6">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="CRAL-TRIO" evidence="3">
        <location>
            <begin position="62"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="253"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="445" mass="51543" checksum="3878B6AB59AB1E4B" modified="2019-05-08" version="1">MDPLSELQDDLNLEDTNQSLSQLKLASLDDKNWPADEVPVFPKSEDTNHSPEPVTHLRWDDPYYDIARHQIVEVAGQGALDEAQSGDDKYGRKVILFSACRMPPSHQLDHVKLLQYLKFTLDQYVESDYTLVYLHHGLTSENKPSLSWLRDAYREFDRKYKKNIKALYIVHPTMFIKTLLILFKPLISFKFGRKIFYANFLGELEEHVKLEQLGIPSQVLKYDEYLKSLQKPSQVPQKPTPPRPPLPNQQFGVSLQHLREKSSDQAPIPLVVRETIAYLREHALTKEGIFRRSASTQIVKEVQQKYNMGIQVDFQQYNDIHLAAVILKTFLRELPEPLLTFSLYNHVVNFQNVEESNRVDVVRKTLQTLPEENYQVLRFLMAYLAEVSTYSDINKMTNTNLAVVFGPNLLWDKDVAITLKAINPINTFTKFLLDYQGEVFQDVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HID6</accession>
    <name>A0A452HID6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HID6"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016759.1">
        <property type="protein sequence ID" value="ENSGAGP00000014657.1"/>
        <property type="gene ID" value="ENSGAGG00000011125.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="102" mass="11675" checksum="FAC76A642C99294F" modified="2019-05-08" version="1">MAKQIQGRKGDVASNLATQAVLFGQTCWKRNKNQNRNDSAPEPAHTVRVWKTACYIILTVLLNDQMRSNSKRLWMLIIGHIFHNCVCIFVPVSHSCPCCHFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIL6</accession>
    <name>A0A452HIL6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptide chain release factor 1-like, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Mitochondrial translational release factor 1-like</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain termination codons UAA and UAG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HIL6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014747"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016855.1">
        <property type="protein sequence ID" value="ENSGAGP00000014747.1"/>
        <property type="gene ID" value="ENSGAGG00000011084.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070126">
        <property type="term" value="P:mitochondrial translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000004">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1660:FF:000004">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1660:FF:000011">
        <property type="entry name" value="Peptide chain release factor 1-like, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1660">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050057">
        <property type="entry name" value="Prokaryotic/Mito_RF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804:SF3">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR 1-LIKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="11">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="381" mass="43296" checksum="FDE0617FE00F1E32" modified="2019-05-08" version="1">GVQRLCRGAAAGAAVCGGRAAQEPRRPLSSWPGLETLFAAPALRRFLEEQAGVRGGPELRARIQRLWAKEQELRDTQELAQADENEDLQKLAEEEIVSCQEEITELKRQIVLHLIPSEEIDNSDLVMEVTAGVGGQEAMLFTAEIFDMYQQYAAYKNWRFEILEYFPSEIGGLRHAAASIAGLEAYKHMKFEGGVHRVQRVPKTEKQGRIHTSTMTIALLPQPMEINLIINPKDLQIETKRASGAGGQHVNTTDSAVRIVHIPTGVVSECQQERSQIRNKEKAMQMLRAKLYSIKLEEETRKRYNARKIQIGTKGRSEKIRTYNFPQDRVTDHRINRTVHHIESFMLGEELLDEMITSLREYADYETLMEIISENVKTNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJ27</accession>
    <name>A0A452HJ27_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJ27"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014909"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017030.1">
        <property type="protein sequence ID" value="ENSGAGP00000014909.1"/>
        <property type="gene ID" value="ENSGAGG00000011273.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000095">
        <property type="entry name" value="immunoglobulin superfamily member 11 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042474">
        <property type="entry name" value="A33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44969">
        <property type="entry name" value="CELL SURFACE A33 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44969:SF1">
        <property type="entry name" value="CELL SURFACE A33 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018990265" description="Ig-like domain-containing protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="20"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="140"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="266"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="284"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="319" mass="34753" checksum="9B279A45E5832839" modified="2019-05-08" version="1">MRATKGSQLFMLSVVLVTAQALTVSTRVKEVKVAQGTNATLPCEFQTSVTPSNADFVTWSKMPQEEVVVTKYFDGIVYASQSYEGRLQFPSTPVTDVSITLRGVTMADNGTYRCTVQLRDELPSASAKIDLLVLVAPSKPECKVIGTSEYGQTISLTCNSELGSPKPEYTWQSFSVLNQPRPMVSASGQQITLKNVSADTSGFYICTATNSVGQEFCNMTVSILPPSMNIALYAGVIGGIVAAIIVIGILAYCCCCRGSKDNDYEMTETGDGGKEPVRIRGPAEEEIEEEEEEEEYRTRERPRTPPTNKPQLEISYAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJ69</accession>
    <name>A0A452HJ69_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HJ69"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014952"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017080.1">
        <property type="protein sequence ID" value="ENSGAGP00000014952.1"/>
        <property type="gene ID" value="ENSGAGG00000011303.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030501">
        <property type="term" value="P:positive regulation of bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045669">
        <property type="term" value="P:positive regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033690">
        <property type="term" value="P:positive regulation of osteoblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031453">
        <property type="entry name" value="TMEM119"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28645">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28645:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15724">
        <property type="entry name" value="TMEM119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019262805" evidence="3">
        <location>
            <begin position="26"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="248" mass="26427" checksum="3E0BEACBC9F0582B" modified="2019-05-08" version="1">IPVWEMAAPATVCVLLMLMAPLCTTRSTRNQATVLDDHSGSGDNPGASILPSASVNLGVNPTSGTIAYMLLIIVVGSLVFVLLFIVCAAVIVRQKHKASAYYPSSFPKKKYVDQNDRSGGAKAFSEVPEKAPETHTEEPMDSSKQLQADILAAAQNLKSPAKVSMANGDGTKIEDKPPKEQEEGTKVEDDKQTAECVSKEEAAPQEKAEPAKETPAPSCTREPAALCRLPQLIVLQLFSLASIFCSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKB4</accession>
    <name>A0A452HKB4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Protein-arginine deiminase</fullName>
            <ecNumber evidence="3 8">3.5.3.15</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the deimination of arginine residues of proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-arginyl-[protein] + H2O = L-citrullyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:18089"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10588"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:83397"/>
            <dbReference type="EC" id="3.5.3.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the protein arginine deiminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.15" evidence="3 8"/>
    <dbReference type="AlphaFoldDB" id="A0A452HKB4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017543.1">
        <property type="protein sequence ID" value="ENSGAGP00000015361.1"/>
        <property type="gene ID" value="ENSGAGG00000011443.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004668">
        <property type="term" value="F:protein-arginine deiminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1700:FF:000001">
        <property type="entry name" value="Protein-arginine deiminase type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.75.10.10:FF:000003">
        <property type="entry name" value="Protein-arginine deiminase type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1700">
        <property type="entry name" value="Protein-arginine deiminase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1860">
        <property type="entry name" value="Protein-arginine deiminase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004303">
        <property type="entry name" value="PAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013530">
        <property type="entry name" value="PAD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036556">
        <property type="entry name" value="PAD_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013732">
        <property type="entry name" value="PAD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038685">
        <property type="entry name" value="PAD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013733">
        <property type="entry name" value="Prot_Arg_deaminase_cen_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10837">
        <property type="entry name" value="PEPTIDYLARGININE DEIMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10837:SF11">
        <property type="entry name" value="PROTEIN-ARGININE DEIMINASE TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03068">
        <property type="entry name" value="PAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08527">
        <property type="entry name" value="PAD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08526">
        <property type="entry name" value="PAD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001247">
        <property type="entry name" value="Protein-arginine_deiminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110083">
        <property type="entry name" value="Peptidylarginine deiminase Pad4, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019460854" description="Protein-arginine deiminase" evidence="10">
        <location>
            <begin position="18"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Protein-arginine deiminase (PAD) N-terminal" evidence="12">
        <location>
            <begin position="55"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Protein-arginine deiminase (PAD) central" evidence="13">
        <location>
            <begin position="155"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Protein-arginine deiminase C-terminal" evidence="11">
        <location>
            <begin position="299"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="495"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="497"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR001247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001247-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="686" mass="76676" checksum="A28888D2E2FDFC81" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKL2</accession>
    <name>A0A452HKL2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000015450"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017650.1">
        <property type="protein sequence ID" value="ENSGAGP00000015450.1"/>
        <property type="gene ID" value="ENSGAGG00000011664.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000026">
        <property type="entry name" value="C-C chemokine receptor type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001277">
        <property type="entry name" value="CXCR4/ACKR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF627">
        <property type="entry name" value="C-C CHEMOKINE RECEPTOR TYPE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00645">
        <property type="entry name" value="CXCCHMKINER4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="52"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="364" mass="41771" checksum="0424E50A172877A9" modified="2019-05-08" version="1">MTFTSPFPNATEYNYVYDEGTSPCSEGNGFHRFKSLFLPIVYCLVFVFCLVGNALLICVLLTRKKVTTMTDVCLLNLAISDLLFVVPLPFQAHYASEEWIFGNAVCKMIAGIYYIGFYSSIFFITLMSIDRYIAIVHAVYGMRVRTATCGIIISLLLWMTAGLAALPNMVFNQEVKIEQSLECVPRYPPGQETWRFFTQFEVNILGLLIPFSILIYCYSHILKNLQSCKNRNKIKAIKLIFIIVVIFFLFWAPFNIVIFLDSLQNLHIINDCETSQRLVLALQLTESISFIHCCLNPVIYAFAGEMFKAHLKKIFQNCIRRIFSSNSLTRHAGKIYCILSFTCTFSSGQWQRWDLDSEVXYILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKS9</accession>
    <name>A0A452HKS9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HKS9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015540"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017752.1">
        <property type="protein sequence ID" value="ENSGAGP00000015540.1"/>
        <property type="gene ID" value="ENSGAGG00000011724.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20340">
        <property type="entry name" value="BRcat_RBR_parkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047534">
        <property type="entry name" value="BRcat_RBR_parkin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003977">
        <property type="entry name" value="Parkin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041170">
        <property type="entry name" value="Znf-RING_14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17978">
        <property type="entry name" value="zf-RING_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01475">
        <property type="entry name" value="PARKIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="104" mass="11594" checksum="453A010146529AD7" modified="2019-05-08" version="1">AGCPDSLIKELHHFRILGEEQYNRYQRYGAEECVLQMGGVLCPTPGCGAGLLPEPEVRKIVCEPSNGLGCGVRLCTFLIAHWESIEIAGFNKKLFKKKRIFLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HM64</accession>
    <name>A0A452HM64_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000016033"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018316.1">
        <property type="protein sequence ID" value="ENSGAGP00000016033.1"/>
        <property type="gene ID" value="ENSGAGG00000012051.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12716">
        <property type="entry name" value="RRM1_2_NP220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033096">
        <property type="entry name" value="ZNF638_RRM1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="841"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="domain" description="Matrin-type" evidence="9">
        <location>
            <begin position="2426"/>
            <end position="2456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="109"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="602"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="743"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="918"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1086"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1331"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1476"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1604"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1738"/>
            <end position="1930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2001"/>
            <end position="2026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2056"/>
            <end position="2082"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2177"/>
            <end position="2416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2456"/>
            <end position="2478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="160"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="342"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="607"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="644"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="930"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="947"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1007"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1086"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1100"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1129"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1331"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1379"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1398"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1430"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1476"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1620"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1740"/>
            <end position="1772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1814"/>
            <end position="1845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1854"/>
            <end position="1864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1882"/>
            <end position="1892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="2056"/>
            <end position="2068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="2180"/>
            <end position="2206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="2216"/>
            <end position="2235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="2238"/>
            <end position="2247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="2259"/>
            <end position="2280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="2292"/>
            <end position="2309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="2326"/>
            <end position="2335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="2336"/>
            <end position="2351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="2371"/>
            <end position="2380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="2464"/>
            <end position="2478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2478" mass="272083" checksum="297B6F216640F0E8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMM4</accession>
    <name>A0A452HMM4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HMM4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016241"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018541.1">
        <property type="protein sequence ID" value="ENSGAGP00000016241.1"/>
        <property type="gene ID" value="ENSGAGG00000012187.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="61" mass="6745" checksum="20324AAE72E745F8" modified="2019-05-08" version="1">MSAAPTTPPSVDKVDGFSRKSVRKARQKRSQSSSQFRSQGKPIELTPLPLLKGKAQDQLYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNJ2</accession>
    <name>A0A452HNJ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HNJ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016556"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018894.1">
        <property type="protein sequence ID" value="ENSGAGP00000016556.1"/>
        <property type="gene ID" value="ENSGAGG00000012380.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17430">
        <property type="entry name" value="MFS_MCT3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004743">
        <property type="entry name" value="MCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00892">
        <property type="entry name" value="2A0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF26">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="426"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="23"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="458"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="514"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="543" mass="58530" checksum="6EA1FCC442830416" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNS3</accession>
    <name>A0A452HNS3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HNS3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016609"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018957.1">
        <property type="protein sequence ID" value="ENSGAGP00000016609.1"/>
        <property type="gene ID" value="ENSGAGG00000012419.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050413">
        <property type="entry name" value="TCR_beta_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268:SF31">
        <property type="entry name" value="T CELL RECEPTOR BETA VARIABLE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268">
        <property type="entry name" value="T-CELL RECEPTOR BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019076005" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="20"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="118" mass="13051" checksum="4231D8E9E548999E" modified="2019-05-08" version="1">MYSVPALLWFLAIVSCIHSATIVQSPQSVVEELGGQISLECSLEGTSNSYIYWYRQLPGRQIHMLAYSVSPGQVDNTGLSSHFNASRPKDDEFLLSINGLLANDTGVYYCAWSRTVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSS2</accession>
    <name>A0A452HSS2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">triacylglycerol lipase</fullName>
            <ecNumber evidence="1">3.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="3.1.1.3" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A452HSS2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018127"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020665.1">
        <property type="protein sequence ID" value="ENSGAGP00000018127.1"/>
        <property type="gene ID" value="ENSGAGG00000013430.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010898">
        <property type="term" value="P:positive regulation of triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07220">
        <property type="entry name" value="Pat_PNPLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000003">
        <property type="entry name" value="Patatin-like phospholipase domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033562">
        <property type="entry name" value="PLPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033903">
        <property type="entry name" value="PNPLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406:SF22">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE PNPLA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 IPLA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="5">
        <location>
            <begin position="10"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="4">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="45"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="4">
        <location>
            <begin position="166"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="504" mass="56030" checksum="1D340F85C9FB7B12" modified="2019-05-08" version="1">MFDRERGWSVSFAGCGFLGVYHIGAATCLQERAPHLIRDARRIFGASAGALTGAVLVGGGSLAEACADVLGLAKEARKRSLGPLHPSFNVIKIIRDGLFKNLPENTHQLSAGKLCISLTRVSDGKNVLVSNFNSKEEIVQALICSSFVPIYCGLIPPSFRGVRYVDGGISDNLPQYESKNTITISPFSGECDICPKGNSANFHEMNVTNTSIQFSLGNLNRLTQALFPPEPKVLGEICEQGYSDALRFLKENDILNDSVYVDLSLKKNPQELIEDTDFTNKKTIPHRKGTEEILKTKMLSNQLKLNTWPLDERIFEHLPPRLRKALQEACKEKSGFCAQVSKLLPMRMMSYLILPYTLPVESAYSVALRLVDWFPDIPDDFQWMQGQLRRIVSTVYSRARNKLLSTSSKESYASLRKCQTVPPNVESHSPYCHSKVPHSAADLEGWLWDFPYCRDSAGKQISVGIKGLSDSDSHHIFFINSEESGLETDFDSSSDKSFQTASED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HT78</accession>
    <name>A0A452HT78_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Exocyst complex component 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Exocyst complex component Sec10</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HT78"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018297"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020852.1">
        <property type="protein sequence ID" value="ENSGAGP00000018297.1"/>
        <property type="gene ID" value="ENSGAGG00000013540.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009976">
        <property type="entry name" value="Sec10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048627">
        <property type="entry name" value="Sec10_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048625">
        <property type="entry name" value="Sec10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100">
        <property type="entry name" value="SEC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07393">
        <property type="entry name" value="Sec10_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20667">
        <property type="entry name" value="Sec10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec10 N-terminal" evidence="9">
        <location>
            <begin position="39"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec10-like alpha-helical bundle" evidence="8">
        <location>
            <begin position="158"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="708" mass="81767" checksum="9EFAD46FD28B37BA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUJ3</accession>
    <name>A0A452HUJ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452HUJ3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018793"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021424.1">
        <property type="protein sequence ID" value="ENSGAGP00000018793.1"/>
        <property type="gene ID" value="ENSGAGG00000013869.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05609">
        <property type="entry name" value="STKc_MAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001045">
        <property type="entry name" value="Microtubule-associated serine/threonine kinase 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000012">
        <property type="entry name" value="microtubule-associated serine/threonine-protein kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1480.20:FF:000001">
        <property type="entry name" value="microtubule-associated serine/threonine-protein kinase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1480.20">
        <property type="entry name" value="MAST3 pre-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037711">
        <property type="entry name" value="MAST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015022">
        <property type="entry name" value="MAST_pre-PK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023142">
        <property type="entry name" value="MAST_pre-PK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF414">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08926">
        <property type="entry name" value="DUF1908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140482">
        <property type="entry name" value="MAST3 pre-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="332"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="95"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="299"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="605" mass="67998" checksum="FFA751F842140B6E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HV03</accession>
    <name>A0A452HV03_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DnaJ homolog subfamily C member 5B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Interacts with the chaperone complex consisting of HSC70 and SGTA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HV03"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018953"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021609.1">
        <property type="protein sequence ID" value="ENSGAGP00000018953.1"/>
        <property type="gene ID" value="ENSGAGG00000014028.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051434">
        <property type="entry name" value="DnaJ_C_subfamily_member5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44027">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44027:SF6">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0564">Palmitate</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="10">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="186" mass="21045" checksum="D983D4A966B2CC8C" modified="2019-05-08" version="1">ESCNLTRHKSLLFKNALFLLFLSCLRKLALKYHPDKNPDNPEAAEKFKELNNAHAILTDLSKRNVYDKYGSLGLYVAEQFGEENVNAYFMLSSWWAKALFAVCGLLTGCYFCCCLCCCFNCCCGQCKPKPPGGEEHEFCMSPEDLEEQIKTDMEQDGETPIILQPTNANEKTQLIGDGHRSYRTDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVP2</accession>
    <name>A0A452HVP2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Bifunctional glycosyltransferase with both alpha-1,3-xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the maturation of alpha-dystroglycan (DAG1) by glycosylation leading to DAG1 binding to laminin G-like domain-containing extracellular proteins with high affinity and in a phosphorylated-O-mannosyl trisaccharide dependent manner. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Supports the maturation of DAG1 more effectively than LARGE1. In addition, can modify both heparan sulfate (HS)- and chondroitin/dermatan sulfate (CS/DS)-proteoglycans (PGs), namely GPC4, with a glycosaminoglycan (GAG)-like polysaccharide composed of xylose and glucuronic acid to confer laminin binding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3-O-[beta-D-GlcA-(1-&gt;3)-beta-D-Xyl-(1-&gt;4)-Rib-ol-P-Rib-ol-P-3-beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-(O-6-P-alpha-D-Man)]-Thr-[protein] + UDP-alpha-D-xylose = 3-O-[alpha-D-Xyl-(1-&gt;3)-beta-D-GlcA-(1-&gt;4)-beta-D-Xyl-(1-&gt;4)-Rib-ol-P-Rib-ol-P-3-beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-(O-6-P-alpha-D-Man)]-Thr-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:57336"/>
            <dbReference type="Rhea" id="RHEA-COMP:17482"/>
            <dbReference type="Rhea" id="RHEA-COMP:17483"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:177336"/>
            <dbReference type="ChEBI" id="CHEBI:177352"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:57337"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>3-O-{(1-&gt;[3)-alpha-D-Xyl-(1-&gt;3)-beta-D-GlcA-(1-&gt;](n)-4)-beta-D-Xyl-(1-&gt;4)-Rib-ol-P-Rib-ol-P-3-beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-O-6-P-alpha-D-Man}-L-Thr-[protein] + UDP-alpha-D-glucuronate = 3-O-{beta-D-GlcA-(1-&gt;[3)-alpha-D-Xyl-(1-&gt;3)-beta-D-GlcA-(1-&gt;](n)-4)-beta-D-Xyl-(1-&gt;4)-Rib-ol-P-Rib-ol-P-3-beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-O-6-P-alpha-D-Man}-L-Thr-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67924"/>
            <dbReference type="Rhea" id="RHEA-COMP:17484"/>
            <dbReference type="Rhea" id="RHEA-COMP:17486"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:177354"/>
            <dbReference type="ChEBI" id="CHEBI:177355"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:67925"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3-O-{beta-D-GlcA-(1-&gt;[3)-alpha-D-Xyl-(1-&gt;3)-beta-D-GlcA-(1-&gt;](n)-4)-beta-D-Xyl-(1-&gt;4)-Rib-ol-P-Rib-ol-P-3-beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-O-6-P-alpha-D-Man}-L-Thr-[protein] + UDP-alpha-D-xylose = 3-O-{(1-&gt;[3)-alpha-D-Xyl-(1-&gt;3)-beta-D-GlcA-(1-&gt;](n+1)-4)-beta-D-Xyl-(1-&gt;4)-Rib-ol-P-Rib-ol-P-3-beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-O-6-P-alpha-D-Man}-L-Thr-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68368"/>
            <dbReference type="Rhea" id="RHEA-COMP:17485"/>
            <dbReference type="Rhea" id="RHEA-COMP:17486"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:177354"/>
            <dbReference type="ChEBI" id="CHEBI:177355"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:68369"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the C-terminal section; belongs to the glycosyltransferase 49 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">In the N-terminal section; belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HVP2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019211"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021889.1">
        <property type="protein sequence ID" value="ENSGAGP00000019211.1"/>
        <property type="gene ID" value="ENSGAGG00000014201.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000021891.1">
        <property type="protein sequence ID" value="ENSGAGP00000019213.1"/>
        <property type="gene ID" value="ENSGAGG00000014201.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042285">
        <property type="term" value="F:xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06431">
        <property type="entry name" value="GT8_LARGE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000229">
        <property type="entry name" value="Glycosyltransferase-like protein LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000016">
        <property type="entry name" value="LARGE xylosyl- and glucuronyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051292">
        <property type="entry name" value="Xyl/GlcA_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270:SF23">
        <property type="entry name" value="XYLOSYL- AND GLUCURONYLTRANSFERASE LARGE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="770" mass="89002" checksum="4D3168A2F9439E5F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HX66</accession>
    <name>A0A452HX66_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sorting nexin-15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May be involved in several stages of intracellular trafficking. Overexpression of SNX15 disrupts the normal trafficking of proteins from the plasma membrane to recycling endosomes or the TGN.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HX66"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019732"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022480.1">
        <property type="protein sequence ID" value="ENSGAGP00000019732.1"/>
        <property type="gene ID" value="ENSGAGG00000014553.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02677">
        <property type="entry name" value="MIT_SNX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000029">
        <property type="entry name" value="sorting nexin-15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051866">
        <property type="entry name" value="Intracell_Sig-Traffick_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15508">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15508:SF9">
        <property type="entry name" value="SORTING NEXIN-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="9">
        <location>
            <begin position="6"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="168"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="176"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="387" mass="43197" checksum="09036F1E5FACDDEC" modified="2019-05-08" version="1">MACTVKDEYQRHYTVTEPRSHPKGYTEYKVTAKFVSKMNPEDVKEITVWKRYSDFKKLHGDLSYIHRNLFRRMEEFPTFPKAQVFGRFEPEVIEERRKAAEAMLRFTVHIPALNNSPQLKEFFRGGEVKRPLEACDLRILPPPLIPVPQEGTDAGDDARGSVAELGRQSLEPEGEPEPHSHQRERAELQSPVPLGAVGVAGEEHNREDKGEETGDRALAAKSEEDLDLLFASVEEEKESGSPLRGPLSNQDLALFDPFIREEQCSASPSQGVMLAVGSELLPSQDIELAADALWDLQISQPAEQEAIQGLDPGGYLAVATEEITLALKREAAKDYAGALCCYRSGVDILLKGVQGDPDPTRREAVKKKTAEYLQRAEELVQLTHLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXH3</accession>
    <name>A0A452HXH3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HXH3"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022689.1">
        <property type="protein sequence ID" value="ENSGAGP00000019911.1"/>
        <property type="gene ID" value="ENSGAGG00000014668.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019124587" evidence="1">
        <location>
            <begin position="25"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="159" mass="18017" checksum="640A9C3E2C2E6651" modified="2019-05-08" version="1">MCLISLCSPLWLLWVQVQVYRAWCKSLTLQRLVYPGGWAACSPPRHTHHQRRSREGFIWLSDLHMAVDLLGLPVSAKQTSQNSHSPHPGHLLGQPSLTNAHVPALSAGQGVFPASGTGVNSHRLADYQTIFDQFPDLLTWKSEEMFHVEDIKWNRKWTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXQ0</accession>
    <name>A0A452HXQ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inhibin beta B chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Activin beta-B chain</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Dimeric, linked by one or more disulfide bonds. Inhibin A is a dimer of alpha and beta-A. Inhibin B is a dimer of alpha and beta-B. Activin A is a homodimer of beta-A. Activin B is a homodimer of beta-B. Activin AB is a dimer of beta-A and beta-B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HXQ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019970"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022752.1">
        <property type="protein sequence ID" value="ENSGAGP00000019970.1"/>
        <property type="gene ID" value="ENSGAGG00000014708.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19405">
        <property type="entry name" value="TGF_beta_INHBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000005">
        <property type="entry name" value="Inhibin beta A chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000012">
        <property type="entry name" value="inhibin beta B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000381">
        <property type="entry name" value="INHBB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF29">
        <property type="entry name" value="INHIBIN BETA B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00671">
        <property type="entry name" value="INHIBINBB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-0372">Hormone</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019545682" description="Inhibin beta B chain" evidence="15">
        <location>
            <begin position="25"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="16">
        <location>
            <begin position="273"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="391" mass="43570" checksum="BBE08D5D7E0A08CA" modified="2019-05-08" version="1">MDGAAWSGVLAALVVCWLAVGAGGSPTPPSPAGGAPPDTCTSCGFRRPEEPGKVDGDFLEAVKRHILSRLQLRDRPNITHAVPKAAMVTALRKLHAGRVREDGQVEIPSLDGQASGGPPLHEQVSEIISFAETDDLASSRVRLYFFISNEGNQNLFVVQASLWLYLKLLPYVLEKGSRRKVRVKVYFQDLDTSNKWNVVEKKVDLKRSGWHTFPMTEAIQSLFEKGERRLNLDVQCEGCEEYSVLPIYVDPGEESHRPFLVVQARLADNKHRIRKRGLECDGRTNLCCRQQFYIDFRLIGWNDWIIAPSGYYGNYCEGSCPAYLAGVPGSASSFHTAVVNQYRMRGLNPGTVNSCCIPTKLSTMSMLYFDDEYNIVKRDVPNMIVEECGCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYE1</accession>
    <name>A0A452HYE1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in cell proliferation and cell motility.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYE1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020186"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023000.1">
        <property type="protein sequence ID" value="ENSGAGP00000020186.1"/>
        <property type="gene ID" value="ENSGAGG00000014887.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03161">
        <property type="entry name" value="TM4SF2_6_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000003">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048232">
        <property type="entry name" value="TSN6/7_LEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF169">
        <property type="entry name" value="TETRASPANIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="251" mass="27861" checksum="5CA4AA5A9FAF3880" modified="2019-05-08" version="1">MASPSQRLQTKPVITCLKSVLLTYSFVFWISGIILLAVGIWGKVGLEVYFSLLNEKATNVPYVLIGTGTVVILLGTFGCFATCRGSTWMLKLYAMFLSLIFLIVLVAAVVGFVFRHEIKNNFESNYSLALKNYNTTMDPHSEAVDTIQRTLHCCGVQNYSDWVNTTYFAQKGIPLSCCKFQNCTESDLKELDKAKAMVYGNGCFSMVTTVMESKMGIVAGISFGIACFQLVGIFLACCLSRYITNNQYEMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYH1</accession>
    <name>A0A452HYH1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Claudin 34</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYH1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020222"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023039.1">
        <property type="protein sequence ID" value="ENSGAGP00000020222.1"/>
        <property type="gene ID" value="ENSGAGG00000014913.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="26792" checksum="A77DE4F6C6397C04" modified="2019-05-08" version="1">MQHTVNLWNSLPEDLVKARTVTGFKKELDKCMEDSTSHLQLAAFAFGTTGWILCTISMGLVEWRVWHVDNTTIISSGIAWVGIWKVCFISYLHVSSDLKEQICHKMNDYETSIPNAIFVAQGLLLIAMVVGALGMASTIFALRSIYMGILYKTQIIRFFLVAGFLYLAAGLCVLIPVSWNFYSVVHNQTISFPPSFYMPSSPVTQEVGAAIRVGIISAILLLMSATFSFSYRFPVTPNAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZH5</accession>
    <name>A0A452HZH5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the wntless family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZH5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020578"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023435.1">
        <property type="protein sequence ID" value="ENSGAGP00000020578.1"/>
        <property type="gene ID" value="ENSGAGG00000015147.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061355">
        <property type="term" value="P:Wnt protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047843">
        <property type="entry name" value="WLS-like_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053936">
        <property type="entry name" value="WLS_GOLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009551">
        <property type="entry name" value="Wntless"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13449">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN GPR177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13449:SF2">
        <property type="entry name" value="PROTEIN WNTLESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06664">
        <property type="entry name" value="WLS-like_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21883">
        <property type="entry name" value="WLS_GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="428"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Wntless GOLD" evidence="11">
        <location>
            <begin position="48"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Wntless-like transmembrane" evidence="10">
        <location>
            <begin position="227"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="541" mass="62218" checksum="DC4A34DE66526491" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I019</accession>
    <name>A0A452I019_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein 93</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC93 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I019"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020789"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023678.1">
        <property type="protein sequence ID" value="ENSGAGP00000020789.1"/>
        <property type="gene ID" value="ENSGAGG00000015303.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039116">
        <property type="entry name" value="CCDC93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019159">
        <property type="entry name" value="CCDC93_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048747">
        <property type="entry name" value="CCDC93_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16441:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16441">
        <property type="entry name" value="FIDIPIDINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09762">
        <property type="entry name" value="CCDC93_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21673">
        <property type="entry name" value="CCDC93_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC93 N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="CCDC93 coiled-coil" evidence="5">
        <location>
            <begin position="119"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="263"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="496"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="567" mass="65658" checksum="20752B5EF79D686A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1N1</accession>
    <name>A0A452I1N1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I1N1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021380"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024352.1">
        <property type="protein sequence ID" value="ENSGAGP00000021380.1"/>
        <property type="gene ID" value="ENSGAGG00000015697.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042804">
        <property type="entry name" value="GPR82"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47392">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 82-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47392:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 82-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="35"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="348" mass="40044" checksum="40AB689DC2BEE7BD" modified="2019-05-08" version="1">MGLLKNSTCLLQPSSASTVALPIIYSFLFTTGSFGNIISGWIFSKNISTKRTQNIYLTNLVIANLFVCITMPFLAAYFADGYQWENDSMLCKIVNYSGTLVIHTSMYVSITILCWTALSQYSTLMKNNDHKQYSPTVNENYFYKCLLEKFRQPKFAKYVCISIWIIVLGVTVPVIVYDLHLQPDQEQIDRCYHWKAENGKLTAKITVLIATAWFFLFFITVLFSYYSLVNHLSKMQKNTCIGKKHLIYSTVKRNIFVILLILTVCYLPYHILRPAFYALLSSEDCQMMNYLVEAKNFLICLAAAKSSLDPVVNLLLDKRFKKSLYNLFRKSSSQDHNHTADIEETTQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I416</accession>
    <name>A0A452I416_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I416"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022277"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025389.1">
        <property type="protein sequence ID" value="ENSGAGP00000022277.1"/>
        <property type="gene ID" value="ENSGAGG00000016364.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15911">
        <property type="entry name" value="7tmA_OR11A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="203"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="44"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="319" mass="36111" checksum="BAB8DE74D529AE01" modified="2019-05-08" version="1">MEKVEGENETYITQFILLGFGIHAELQPLLFLLFLVIYLVTMAGNILIIALVVTDLHLHTPMYFFLGNLSCLETSFTSTILPRVLASLLTGDRNISVTGCITQFYFLGLFLGVECYLLALMSYDRYLAICKPLHYTGLMNIRICLQLAAVSWMSGFMIITIVTCLMSQLHFCGPNEINHFLCDFTPVIKLSCSDTSLITLVTFIFSSIYTLPLFLLTLASYICIISTILRIPSTTKRRKAFSTCSSHLIVVTIYYGTLIIVYMLPDTDTLRDLNKVFSLFYTVLTPLVNPLIYSLRNKEVKVALRKVISKYMALIEIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6N9</accession>
    <name>A0A452I6N9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000023196"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026431.1">
        <property type="protein sequence ID" value="ENSGAGP00000023196.1"/>
        <property type="gene ID" value="ENSGAGG00000017007.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001806">
        <property type="entry name" value="rho-related GTP-binding protein RhoD isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="188" mass="20498" checksum="7FA2F1EB2F65FC1E" modified="2019-05-08" version="1">MLSWYTGGLSLRAGRLLASLGASLQVRRYHILATPLAVPGAWSATQQPCSGLGQMXSPGVHLPVQAGGAGQGLIWVPDADPAPCAENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMAGRISAFGYLECSAKTKDGVREVFEMATRAALQERGWQEEIGHRASPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8J7</accession>
    <name>A0A452I8J7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TELO2-interacting protein 1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Regulator of the DNA damage response (DDR). Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins. The TTT complex is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and mitomycin C (MMC). Together with the TTT complex and HSP90 may participate in the proper folding of newly synthesized PIKKs. Promotes assembly, stabilizes and maintains the activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the tti1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I8J7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023855"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027171.1">
        <property type="protein sequence ID" value="ENSGAGP00000023855.1"/>
        <property type="gene ID" value="ENSGAGG00000017457.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000229">
        <property type="entry name" value="TELO2-interacting protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000240">
        <property type="entry name" value="TELO2-interacting protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052587">
        <property type="entry name" value="TELO2-interacting_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057567">
        <property type="entry name" value="TPR_TTI1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057566">
        <property type="entry name" value="TPR_TTI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016441">
        <property type="entry name" value="Tti1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049362">
        <property type="entry name" value="TTI1_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460">
        <property type="entry name" value="TEL2 INTERACTING PROTEIN 1 TTI1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460:SF3">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24176">
        <property type="entry name" value="TPR_TTI1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24181">
        <property type="entry name" value="TPR_TTI1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24173">
        <property type="entry name" value="TPR_TTI1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21547">
        <property type="entry name" value="TTI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005250">
        <property type="entry name" value="UCP005250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="TTI1 N-terminal TPR" evidence="10">
        <location>
            <begin position="11"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="TTI1 C-terminal TPR" evidence="11">
        <location>
            <begin position="760"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="782"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="830"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1097"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="783"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1109"/>
            <end position="1118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00056900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1118" mass="123877" checksum="C69B18EF67238696" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I965</accession>
    <name>A0A452I965_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox protein GBX-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Gastrulation and brain-specific homeobox protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May act as a transcription factor for cell pluripotency and differentiation in the embryo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000024212"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027564.1">
        <property type="protein sequence ID" value="ENSGAGP00000024212.1"/>
        <property type="gene ID" value="ENSGAGG00000017701.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051960">
        <property type="term" value="P:regulation of nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000248">
        <property type="entry name" value="Gastrulation brain homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042982">
        <property type="entry name" value="GBX-1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24334">
        <property type="entry name" value="HOMEOBOX PROTEIN GBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24334:SF3">
        <property type="entry name" value="HOMEOBOX PROTEIN GBX-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="248"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="250"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="63"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="179"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="234"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="351" mass="38538" checksum="2950DDE03717780B" modified="2019-05-08" version="1">MSAAFQPSLMMMQRPLGSSTAFSIDSLIGSPPQPSPGHFVYTGYPMFMPYRPVVLPPXXXXXXXXXXPPPPTLPQASLQPALPPSHPHHQIPSLPTGFCSSLAQGMALTSTLMATLPSSFSASPQHQEAARKFAPQALQGNFDKGEGIQQETEEGKSFLSKERSLLSFAAAEAVQASLGAVRPQAKDDSKAEDDSKGKEESFSMDSDLDYSSDDNITSQAAHKEEDSGNALEENTQSTNNSNTTTSTGKNRRRRTAFTSEQLLELEKEFHCKKYLSLTERSQIAHALKLSEVQVKIWFQNRRAKWKRVKAGNANSKTGEPSRNPKIVVPIPVHVSRFAIRSQHQQLEQARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9S5</accession>
    <name>A0A452I9S5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I9S5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024438"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027813.1">
        <property type="protein sequence ID" value="ENSGAGP00000024438.1"/>
        <property type="gene ID" value="ENSGAGG00000017855.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF118">
        <property type="entry name" value="ZINC FINGER PROTEIN 746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="24"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="94" mass="10589" checksum="79BAEDA5E57FA4D4" modified="2019-05-08" version="1">GPPCACVLSEGQADHKPSGTSVPVTFDDVSVYFNEQEWENLEEWQKELYKNVMKENYDTLISLGRARVLLVYFQLSLTASGTVLSDQIHTLSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAE4</accession>
    <name>A0A452IAE4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="13">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A452IAE4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024499"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027881.1">
        <property type="protein sequence ID" value="ENSGAGP00000024499.1"/>
        <property type="gene ID" value="ENSGAGG00000017897.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904154">
        <property type="term" value="P:positive regulation of retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000016">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF5">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0834">Unfolded protein response</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="567"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="584"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="440"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="459"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="545"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="593" mass="66852" checksum="0A34029062177E79" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAN2</accession>
    <name>A0A452IAN2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">eEF1A lysine and N-terminal methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Methyltransferase-like protein 13</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IAN2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024767"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028184.1">
        <property type="protein sequence ID" value="ENSGAGP00000024767.1"/>
        <property type="gene ID" value="ENSGAGG00000018083.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000150">
        <property type="entry name" value="methyltransferase-like protein 13 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051419">
        <property type="entry name" value="Lys/N-term_MeTrsfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037959">
        <property type="entry name" value="MFS_SpdSyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176:SF78">
        <property type="entry name" value="EEF1A LYSINE AND N-TERMINAL METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="8">
        <location>
            <begin position="7"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="364"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="631" mass="70442" checksum="B78415DD19636426" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAT8</accession>
    <name>A0A452IAT8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone deacetylase</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452IAT8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024829"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028250.1">
        <property type="protein sequence ID" value="ENSGAGP00000024829.1"/>
        <property type="gene ID" value="ENSGAGG00000018128.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd10163">
        <property type="entry name" value="ClassIIa_HDAC9_Gln-rich-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024643">
        <property type="entry name" value="Hist_deacetylase_Gln_rich_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364:SF11">
        <property type="entry name" value="HISTONE DEACETYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364">
        <property type="entry name" value="HISTONE DEACETYLASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12203">
        <property type="entry name" value="HDAC4_Gln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase glutamine rich N-terminal" evidence="12">
        <location>
            <begin position="65"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="141"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="244"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="293"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="528"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="141"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="317"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="538"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="622" mass="69819" checksum="B16DD837CDEA7788" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB09</accession>
    <name>A0A452IB09_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AP-3 complex subunit delta Mu C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IB09"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024874"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028310.1">
        <property type="protein sequence ID" value="ENSGAGP00000024874.1"/>
        <property type="gene ID" value="ENSGAGG00000018173.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058898">
        <property type="entry name" value="Mu_AP3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26171">
        <property type="entry name" value="Mu_AP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP-3 complex subunit delta Mu C-terminal" evidence="1">
        <location>
            <begin position="114"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="221" mass="24464" checksum="29E50D559CA55348" modified="2019-05-08" version="1">MTYDIQGNLQNDSQVTVSVIFENKSTSFLKSMELNVLDSLNAKMLRPEGSSVHDGIPVPFQLPPGISNEAQFVFTLQSIVMAQKLKGTLSFIVKNDNGSTHEKLDFKLHFSCASYLITTPCYSDAFAKLLESGDLHMSSIKVDGISISFQHLLAKICFYHHFSVVERVDSCASMYSRSIQGHHVCLLVKKGENSVSVDGKCNDSTLLSNLLDEMKETLSKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBF3</accession>
    <name>A0A452IBF3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IBF3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025019"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028480.1">
        <property type="protein sequence ID" value="ENSGAGP00000025019.1"/>
        <property type="gene ID" value="ENSGAGG00000018293.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18197">
        <property type="entry name" value="BTB_POZ_ZBTB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000333">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000047">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF29">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="12">
        <location>
            <begin position="33"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="380"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="424" mass="48558" checksum="A3771AAE450F8E42" modified="2019-05-08" version="1">MATDSDVLHFQFEQQGDAVLQKMNLLRQQNLFCDVSIYINDTEFHGHKVIFAACSTFMRDQFLLNQSKQMRITILQSAEVGRKLLLSCYTGALEVKKKELLKYLIAASYLQMVHIVEKCTEALSKYLEIDASMENSNQNTEKCHSSDTELRNKDDSLLKDCEIIEISEDSPVNVEYNVKREKGDVLQPAMQSLISERKDMKTPEISTVEIGYKDDEICIFRMDSMNTANVENDHFPQPCTSSKTSLYFPETQHSLINSTVESRITEVPGSHFQGFVSDNTEGTSGGFQSLEDSGYSWRHQCPKCPRGFIHLENYLRHLKMHKLFLCLQCGKTFTQKKNLNRHIRGHMGIRPFQCMVCLKTFTAKSTLQDHLNIHSGDRPYKCHCCDMDFKHKSALKKHLTSVHGRNGGDKPNLDAITQIKIDYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICU2</accession>
    <name>A0A452ICU2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ICU2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025588"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029116.1">
        <property type="protein sequence ID" value="ENSGAGP00000025588.1"/>
        <property type="gene ID" value="ENSGAGG00000018691.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040311">
        <property type="entry name" value="CCDC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31663">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31663:SF4">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019212554" evidence="2">
        <location>
            <begin position="19"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="189"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="277" mass="32278" checksum="170BA33BC5A4B314" modified="2019-05-08" version="1">MLHMLLLLLLLLLPLGRQLSPCWGCQLPSDWRPLSESCRAELAEIIVYAKVLALHQEMYSLYNYLPWQYEASEGGLFYSAEIEMLCDQAWGSMLEVPAGSRLNLTGLGYFSCHSHTVMQDYSYFFFLRMDENYNLLPHGVNFQDAIFPDTQENRRMFSSLFQFSNCSQAQHVITFSSDWEIQEDNRLMCSSVQKALFEEEDQVKKLQQKVATLEKRNKQLRDRVKKVKRSLRQARKNNRHMEQINRKLSEKLSSAGGQIPHINSLIQENSHAIYLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IED2</accession>
    <name>A0A452IED2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL30</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IED2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026217"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029814.1">
        <property type="protein sequence ID" value="ENSGAGP00000026217.1"/>
        <property type="gene ID" value="ENSGAGG00000019136.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.15.30:FF:000001">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.15.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005998">
        <property type="entry name" value="Ribosomal_uL30_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012988">
        <property type="entry name" value="Ribosomal_uL30_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01310">
        <property type="entry name" value="uL30_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF13">
        <property type="entry name" value="RIBOSOMAL PROTEIN UL30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08079">
        <property type="entry name" value="Ribosomal_L30_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30 N-terminal eukaryotes" evidence="7">
        <location>
            <begin position="28"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="6">
        <location>
            <begin position="104"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="262" mass="30130" checksum="B3E4F2BC3D7C50E1" modified="2019-05-08" version="1">MRTSGMTPDSGGGGTMAEPEPKRKIPLVPENLLKKRKAYQALKATQAKQALLEKRKHQKGKQIQFKRIEIFVRDSRRKHKDEVRLKRMEQKPGQMVLPQGPKLAFVVRIAEIKGVSLRVRRVIQMLRLRKIFSGTFVKLTPASLKMLRTVEPYVAWGHPNLKSVRELILKRGQAKINKKRVPLTDNILIEKHLGKFDIICLEDLIHEIYSAGKHFREITNFLWPFHLSVARHAACNKVGFVKEIGEPGYRGEGINKLIRQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFR9</accession>
    <name>A0A452IFR9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphodiesterase 10A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IFR9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026738"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030387.1">
        <property type="protein sequence ID" value="ENSGAGP00000026738.1"/>
        <property type="gene ID" value="ENSGAGG00000019472.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="23151" checksum="564CDB47278F530E" modified="2019-05-08" version="1">MRSLPQVPPSQLPLAGNCGGGACRWGSAQSHLAALLLICIGAGGGHAGCFQELFEVSLTDEKVKAYLSLHPQVLDEFVSESVSAETVEKWLKRKNNRQEDESTPKEVSRYQDTNMQGVVYELNSYIEQRLDTGGDNQLLLYELSSIIKIGRTTTAHSCRAHCTWHHNICICSQIQKNFVSRRYSGGKLLSLFKEIHAFFFQKMYYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGH5</accession>
    <name>A0A452IGH5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein YIPF</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the maintenance of the Golgi structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the YIP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IGH5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027068"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030758.1">
        <property type="protein sequence ID" value="ENSGAGP00000027068.1"/>
        <property type="gene ID" value="ENSGAGG00000019697.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF7">
        <property type="entry name" value="PROTEIN YIPF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="9">
        <location>
            <begin position="109"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="249" mass="27466" checksum="255598F21006BDD6" modified="2019-05-08" version="1">MQPPGPQQPPLYAPGNGDFTFVSSAEAEDLSGSIASPDVKLNLGGGGGEFIKESTATTFLRQRGYGWLLEVEDDDPEDNKPLLEELDIDLKDIYYKIRCVLMPMPSLGFNRQVVRDNPDFWGPLAVVLFFSMVSLYGQFKVVSWIITIWIFGSLTIFLLARVLGGEVAYGQVLGVIGYSLLPLIVIAPVLLVVGSFEAVSTLIKLFGVFWAAYSAASLLVGEEFKTKKPLLIYPIFLLYIYFLSLYTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJQ3</accession>
    <name>A0A452IJQ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc finger protein</fullName>
            <ecNumber evidence="17">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="16">Interacts with EHMT2/G9A, GFI1 and HDAC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A452IJQ3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028034"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031842.1">
        <property type="protein sequence ID" value="ENSGAGP00000028034.1"/>
        <property type="gene ID" value="ENSGAGG00000020362.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19190">
        <property type="entry name" value="PR-SET_PRDM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000015">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000407">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000476">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000510">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000518">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000636">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000659">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000546">
        <property type="entry name" value="Zinc finger protein 333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044415">
        <property type="entry name" value="PRDM5_PR-SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017125">
        <property type="entry name" value="Znf_PRDM5-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF70">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037162">
        <property type="entry name" value="PRDM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="21">
        <location>
            <begin position="8"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="177"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="209"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="272"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="442"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="471"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="525"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="553"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="128"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR037162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="584" mass="67111" checksum="CEFACC1F946CDD28" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJU5</accession>
    <name>A0A452IJU5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Poly(A) polymerase</fullName>
            <ecNumber evidence="14">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Polymerase that creates the 3'-poly(A) tail of mRNA's.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="16">Binds 2 magnesium ions. Also active with manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A452IJU5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028206"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032054.1">
        <property type="protein sequence ID" value="ENSGAGP00000028206.1"/>
        <property type="gene ID" value="ENSGAGG00000020500.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000002">
        <property type="entry name" value="Poly(A) polymerase alpha, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.590:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007012">
        <property type="entry name" value="PolA_pol_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048840">
        <property type="entry name" value="PolA_pol_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007010">
        <property type="entry name" value="PolA_pol_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014492">
        <property type="entry name" value="PolyA_polymerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682">
        <property type="entry name" value="POLY A POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682:SF9">
        <property type="entry name" value="POLY(A) POLYMERASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04928">
        <property type="entry name" value="PAP_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20750">
        <property type="entry name" value="PAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04926">
        <property type="entry name" value="PAP_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018425">
        <property type="entry name" value="PolyA_polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) polymerase nucleotidyltransferase" evidence="20">
        <location>
            <begin position="21"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase central" evidence="19">
        <location>
            <begin position="219"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase RNA-binding" evidence="18">
        <location>
            <begin position="366"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="523"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="535"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="549"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="581"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="614"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="649"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="100"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="113"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="246"/>
            <end position="247"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR018425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR018425-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR018425-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF20750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="668" mass="75029" checksum="05648921F92CB14C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IP95</accession>
    <name>A0A452IP95_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA repair protein RAD51 homolog 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">RAD51 homolog B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">RAD51-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel. The BCDX2 subcomplex RAD51B:RAD51C interacts with RAD51. Interacts with SWSAP1; involved in homologous recombination repair. Interacts with HELQ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RecA family. RAD51 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IP95"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029714"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033740.1">
        <property type="protein sequence ID" value="ENSGAGP00000029714.1"/>
        <property type="gene ID" value="ENSGAGG00000021444.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
        <property type="term" value="C:Rad51B-Rad51C-Rad51D-XRCC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000806">
        <property type="entry name" value="DNA repair protein RAD51 homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058766">
        <property type="entry name" value="HHH_XRCC3_RAD51B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030548">
        <property type="entry name" value="RAD51B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46456">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46456:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26169">
        <property type="entry name" value="HHH_XRCC3_RpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="15">
        <location>
            <begin position="79"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="359" mass="40040" checksum="BFD3995632DDAE43" modified="2019-05-08" version="1">MATKKLRRAGLSQELCDRLSRHQIITCRDFLCLSLLELMKVTGKSYWNVQKLLCKVSLACAPKMQTAYEMKMKTAINPSSAFLSTTLNGLDKALHGGVACGSLTEVTGPSGCGKTQFCLMMSVLATLPISMGGLDGAVIYIDTESAFGAERLVEIAEHRFPNYFGTEEKLFSMTRSIHLYRELTCDSVLKRIKTLEEEIISKKVKLVIIDSVASVVRKEFDTKLQGNLMERSNFLAREASLLKYLAEEFSIPVSFSFFFFFKSLAHSIKLGLPGPSAVIVSLGSGAVPFLYELKYPQICGRKIVTVHRAPGHRLISKPTVPLTRASVPYQDWVRLFKVVLQDVMALNERRESSDIKKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPA3</accession>
    <name>A0A452IPA3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-sulfur cluster assembly 1 homolog, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IPA3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029932"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033991.1">
        <property type="protein sequence ID" value="ENSGAGP00000029932.1"/>
        <property type="gene ID" value="ENSGAGG00000021603.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.300.12:FF:000001">
        <property type="entry name" value="Iron-binding protein IscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050322">
        <property type="entry name" value="Fe-S_cluster_asmbl/transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072:SF41">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Core" evidence="4">
        <location>
            <begin position="23"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="124" mass="13665" checksum="F4F46CB02E4963C3" modified="2019-05-08" version="1">MASSVVRATVRAVSKRKIQATRTPSAVHKIKQLLKDKPDHVGVKVGVRTRGCNGLSYTLDYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYVEDKLSSEFVFNNPNIKGTCGCGESFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ISI4</accession>
    <name>A0A452ISI4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Phosphoinositide 3-kinase regulatory subunit 4</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 30">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 30">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="22">Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxiliary subunits to form alternative complex forms. Alternative complex forms containing a fourth regulatory subunit in a mutually exclusive manner are PI3K complex I (PI3KC3-C1) containing ATG14, and PI3K complex II (PI3KC3-C2) containing UVRAG. PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex. Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits, such as RUBCN, SH3GLB1/Bif-1, AMBRA1 and NRBF2. PI3KC3-C1 probably associates with PIK3CB. Interacts with RAB7A in the presence of PIK3C3/VPS34. Interacts with NRBF2. Interacts with ARMC3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452ISI4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030982"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035129.1">
        <property type="protein sequence ID" value="ENSGAGP00000030982.1"/>
        <property type="gene ID" value="ENSGAGG00000022264.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000035131.1">
        <property type="protein sequence ID" value="ENSGAGP00000030984.1"/>
        <property type="gene ID" value="ENSGAGG00000022264.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13980">
        <property type="entry name" value="STKc_Vps15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000305">
        <property type="entry name" value="phosphoinositide 3-kinase regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000100">
        <property type="entry name" value="phosphoinositide 3-kinase regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000154">
        <property type="entry name" value="Phosphoinositide 3-kinase regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000319">
        <property type="entry name" value="Phosphoinositide 3-kinase regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045162">
        <property type="entry name" value="Vps15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583:SF0">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 25" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="26"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="24">
        <location>
            <begin position="417"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="24">
        <location>
            <begin position="462"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="25">
        <location>
            <begin position="990"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="864"/>
            <end position="893"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00053507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00062774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00073019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1178" mass="133106" checksum="726FF1A0211A0240" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITM9</accession>
    <name>A0A452ITM9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Neurexophilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May be signaling molecules that resemble neuropeptides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the neurexophilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ITM9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031314"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035516.1">
        <property type="protein sequence ID" value="ENSGAGP00000031314.1"/>
        <property type="gene ID" value="ENSGAGG00000022503.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010450">
        <property type="entry name" value="Nxph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026845">
        <property type="entry name" value="NXPH/NXPE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17103">
        <property type="entry name" value="NEUREXOPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17103:SF11">
        <property type="entry name" value="NEUREXOPHILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06312">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038019">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="265" mass="30350" checksum="5E8A038743E54872" modified="2019-05-08" version="1">MMYLQPLPLVVVQSFLQLIFCDTKQTVHATDVLDWEDKDVAETLVNNVAHSRIINPLRLFVKPSPVLKHGQMSYSDSIENFWDWLANITEVQESLARTKRRPIVKTGKFKKMFGWGDFHSNIKTVKLNLLITGKIVDHGNGTFSVYFRHNSTGLGNVSVSLVPPSKVVEFESSPQSTLETKESKSFNCRIEYEKTDRAKKTALCNFDPSKICYQEQTQSHVSWLCSKPFKVICIYIAFYSVDYKLVQKVCPDYNYHSETPYLSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUQ3</accession>
    <name>A0A452IUQ3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IUQ3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031737"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035993.1">
        <property type="protein sequence ID" value="ENSGAGP00000031737.1"/>
        <property type="gene ID" value="ENSGAGG00000022799.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046580">
        <property type="term" value="P:negative regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13369">
        <property type="entry name" value="PH_RASAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05135">
        <property type="entry name" value="RasGAP_RASAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000283">
        <property type="entry name" value="Ras GTPase-activating protein 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037776">
        <property type="entry name" value="RASAL_RasGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001562">
        <property type="entry name" value="Znf_Btk_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF3">
        <property type="entry name" value="RASGAP-ACTIVATING-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00779">
        <property type="entry name" value="BTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51113">
        <property type="entry name" value="ZF_BTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="116"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="10">
        <location>
            <begin position="307"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="572"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="787"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="819" mass="93210" checksum="5C565F64A5327554" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IVQ8</accession>
    <name>A0A452IVQ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Homeobox-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Binds directly to 5'-TTAGGG-3' repeats in telomeric DNA. Associates with the telomerase complex at sites of active telomere processing and positively regulates telomere elongation. Important for TERT binding to chromatin, indicating a role in recruitment of the telomerase complex to telomeres. Also plays a role in the alternative lengthening of telomeres (ALT) pathway in telomerase-negative cells where it promotes formation and/or maintenance of ALT-associated promyelocytic leukemia bodies (APBs). Enhances formation of telomere C-circles in ALT cells, suggesting a possible role in telomere recombination. Might also be involved in the DNA damage response at telomeres.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Associates with the telomerase holoenzyme complex. Interacts with DKC1, XRCC6 and COIL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Cajal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">PML body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IVQ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031981"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036261.1">
        <property type="protein sequence ID" value="ENSGAGP00000031981.1"/>
        <property type="gene ID" value="ENSGAGG00000022936.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000038">
        <property type="entry name" value="Homeobox-containing protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000011">
        <property type="entry name" value="homeobox-containing protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040363">
        <property type="entry name" value="HMBOX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006899">
        <property type="entry name" value="HNF-1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044869">
        <property type="entry name" value="HNF-1_POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044866">
        <property type="entry name" value="HNF_P1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14618:SF0">
        <property type="entry name" value="HOMEOBOX-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14618">
        <property type="entry name" value="HOMEODOX-CONTAINING PROTEIN 1 HMBOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04814">
        <property type="entry name" value="HNF-1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51937">
        <property type="entry name" value="HNF_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51936">
        <property type="entry name" value="POU_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 19 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13 19 20" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="15 19 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0779">Telomere</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="HNF-p1" evidence="24">
        <location>
            <begin position="18"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="POU-specific atypical" evidence="23">
        <location>
            <begin position="146"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="22">
        <location>
            <begin position="266"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="19">
        <location>
            <begin position="268"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="56"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="354"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="383"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00064808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00072740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="455" mass="51425" checksum="78F68F0665355EDF" modified="2019-05-08" version="1">MLRTFPVVLLETMSHYTDEPRFTIEQIDLLQRLRRTGMTRHEILHALETLDRLDQEHSDKFGRRSSYGGGSYGNSTNNVPASSSTATASTQTQHSGMSPSPSNSYDTSPQPCTTNQNGRESNERLSAFNGKMSPTRYPLANSLAQRSYSFEASEEDLDVDDKVEELMRRDSSVIKEEIKAFLANRRISQAVVAQVTGISQSRISHWLLQQGSDLSEQKKRAFYRWYQLEKTNPGATLSMRPAPIPVEEPEWRQTPPPVTATSGTFRLRRGSRFTWRKECLAVMESYFNENQYPDEAKREEIANACNAVIQKPGKKLSDLERVTSLKVYNWFANRRKEIKRRANIEAAILESHGIDVQSPGGHSNSDDVDGNDYSEQDTWQVRNGEEEGRCSEGGREAEKVEEDRRICSKQDDSTSHSDHQDPISLAVEMAAVNHTILALARQGTNEIKTEALDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IXS5</accession>
    <name>A0A452IXS5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IXS5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032881"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037270.1">
        <property type="protein sequence ID" value="ENSGAGP00000032881.1"/>
        <property type="gene ID" value="ENSGAGG00000023490.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058353">
        <property type="entry name" value="DUF8040"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF206">
        <property type="entry name" value="NUCLEASE HARBI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26138">
        <property type="entry name" value="DUF8040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8040" evidence="10">
        <location>
            <begin position="91"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="DDE Tnp4" evidence="9">
        <location>
            <begin position="204"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF26138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="421" mass="47956" checksum="2212781B05BD0827" modified="2019-05-08" version="1">MDPEQLTTLLMNVASTSRMAVELFLQLQNESESDSDSESEESDDDIESPHCEDSKLLVAVTDVLSSVDRRVWARETSTQWWDQIVLQSWDDEQWLQNFRMRKATFMALCAELAPTLRRRDTRLRAALPVEKRVAIAIWKLATPDSFRSVANQFGVGKSTVGMVLMQVCTAINRTLLRRTVTLGNVQDIVDGFAQMGFPNCGGAIDGTHIPILSPPHLASEYVNRKGYFSVVLQALVNHRGRFTDIYSGWPGKVHDARIFRNSALFRRLRAGTFFPDHKITVGDVEMPTVILGDPAYPLMPWLMKPYTGKLDRSKDRFNYRLSRCRMTVECAFGRLKARWRCLYGKLDLGESSISAVISACCTLHNICEGKGERFSEEWTSEVRRLEDEFAQPESRANREAQERASRIRDALREQFDAESQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYY9</accession>
    <name>A0A452IYY9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G protein-activated inward rectifier potassium channel 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Inward rectifier K(+) channel Kir3.1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Potassium channel, inwardly rectifying subfamily J member 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYY9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033277"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037705.1">
        <property type="protein sequence ID" value="ENSGAGP00000033277.1"/>
        <property type="gene ID" value="ENSGAGG00000023721.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015467">
        <property type="term" value="F:G-protein activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000019">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000006">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003274">
        <property type="entry name" value="K_chnl_inward-rec_Kir3.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF16">
        <property type="entry name" value="G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01327">
        <property type="entry name" value="KIR31CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 17" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 17" id="KW-0813">Transport</keyword>
    <keyword evidence="7 17" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="20">
        <location>
            <begin position="42"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="21">
        <location>
            <begin position="189"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="16">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005465-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="303" mass="34596" checksum="7EEFF2660E991824" modified="2019-05-08" version="1">MSALRRKFGDDYQVVTTSSSGSGFHQQQAAAAAPRRKRQRFVDKNGRCNVQHGNLGSETSRYLSDLFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWLIAYMRGDLNRAHDDNYTPCVANVYNFPSAFLFFIETEATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNLRNSHMVSAQIRCKLLKSRQTPEGEFLPLDQLELDVGFSTGADQLFLVSPLTICHVIDAKSPFYDLSQRSMQTEQFEIVVILEGIVETTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZ83</accession>
    <name>A0A452IZ83_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cbp/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CITED family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZ83"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033402"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037843.1">
        <property type="protein sequence ID" value="ENSGAGP00000033402.1"/>
        <property type="gene ID" value="ENSGAGG00000023799.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2200:FF:000001">
        <property type="entry name" value="Cbp/p300-interacting transactivator 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007576">
        <property type="entry name" value="CITED"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17045:SF5">
        <property type="entry name" value="CBP_P300-INTERACTING TRANSACTIVATOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17045">
        <property type="entry name" value="MELANOCYTE SPECIFIC GENE RELATED CITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04487">
        <property type="entry name" value="CITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="22739" checksum="06874CBECEC38B18" modified="2019-05-08" version="1">MADHMMMSMSHGVNGLQNYRMGMNGLQGPPQHGQHVLRTLPTTNQMMQYGGASMDGSMRPRPNISGQMGHHQMPNTMMFNSPSQQQQYLGPVGTQQLMASMHLQKLNTQYQGHPLMGMNNGPMAAGAQQYRVVPSQHPGMQHMPSPALTLNVMDTDLIDEEVLTSLVQELGLDRIQELPELFLGQNEFDFISDFVSKQQPSAISC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0Q3</accession>
    <name>A0A452J0Q3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Dual specificity tyrosine-phosphorylation-regulated kinase 2</fullName>
            <ecNumber evidence="6">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452J0Q3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033942"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038438.1">
        <property type="protein sequence ID" value="ENSGAGP00000033942.1"/>
        <property type="gene ID" value="ENSGAGG00000024135.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14224">
        <property type="entry name" value="PKc_DYRK2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.10.30:FF:000001">
        <property type="entry name" value="Dual specificity tyrosine-phosphorylation-regulated kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000112">
        <property type="entry name" value="Putative dual specificity tyrosine-phosphorylation-regulated kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000127">
        <property type="entry name" value="Putative dual specificity tyrosine-phosphorylation-regulated kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.10.30">
        <property type="entry name" value="DYRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042521">
        <property type="entry name" value="DYRK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF51">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="14 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0464">Manganese</keyword>
    <keyword evidence="12 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="147"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00069142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="526" mass="59408" checksum="9092DB7EEEBDC6FD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1H6</accession>
    <name>A0A452J1H6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A</fullName>
            <ecNumber evidence="6">2.4.99.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] = N(4)-(oligosaccharide-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein] + a di-trans,poly-cis-dolichyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22980"/>
            <dbReference type="Rhea" id="RHEA-COMP:12804"/>
            <dbReference type="Rhea" id="RHEA-COMP:12805"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="Rhea" id="RHEA-COMP:19509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57570"/>
            <dbReference type="ChEBI" id="CHEBI:132529"/>
            <dbReference type="EC" id="2.4.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Component of the oligosaccharyltransferase (OST) complex. There are 2 OST complexes, OST-A and OST-B, which contain STT3A or STT3B as catalytic subunit, respectively. OST-A and OST-B contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, and OST-A contains DC2/OSTC and KRTCAP2/KCP2 specific accessory subunits. OST-A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the OST-A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The OST-A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.18" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452J1H6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034233"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038760.1">
        <property type="protein sequence ID" value="ENSGAGP00000034233.1"/>
        <property type="gene ID" value="ENSGAGG00000024315.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004579">
        <property type="term" value="F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043687">
        <property type="term" value="P:post-translational protein modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12610:FF:000002">
        <property type="entry name" value="dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054479">
        <property type="entry name" value="AglB-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF43">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22627">
        <property type="entry name" value="AglB_core-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="192"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="21">
        <location>
            <begin position="17"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="AglB-like core" evidence="22">
        <location>
            <begin position="512"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF22627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="705" mass="80608" checksum="246C2EC9AB2CBCEF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J3J6</accession>
    <name>A0A452J3J6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J3J6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035002"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039631.1">
        <property type="protein sequence ID" value="ENSGAGP00000035002.1"/>
        <property type="gene ID" value="ENSGAGG00000024881.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF206">
        <property type="entry name" value="NUCLEASE HARBI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="219"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="393" mass="44767" checksum="2FA5A7BE6D6EEDBA" modified="2019-05-08" version="1">MVLRYTFQAVWHHCESAARFLLQMEPELLRTLLMNVASTSRLAVELFLQLQSDSEESDDDIDSPDAQDTKLLVAVTDVLSTVERCLWAQETSTEWWDHIVLQVWDDEQWLQNFRMRKATFMGLCEELAPTLRRKDTRLRAALPVEKWVAIAIWKLATPDGYRSVANQFGVEKSTVGIVLMQVCTAINRTLLRKTVTLGNVQDIVDGFAEMGFPNCGGAIDGTHIPILSPPHLASEYVNRKGYFSVVLQALVDHRGRFTDIYSGWPGKVHDARIFRNSALFRRLRAGTFFPDRKITVGDVEMPTVILGDPAYPLMPWLMKPYTGKLDRSKDRFNYRLSRCRMTVECAFGRLKARWRCLYGKLDLGESSISAVISACCTLHNICEGKGETFSEEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5E2</accession>
    <name>A0A452J5E2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J5E2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035703"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040427.1">
        <property type="protein sequence ID" value="ENSGAGP00000035703.1"/>
        <property type="gene ID" value="ENSGAGG00000025349.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01209">
        <property type="entry name" value="PTB_Shc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09925">
        <property type="entry name" value="SH2_SHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000036">
        <property type="entry name" value="SHC-transforming protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000005">
        <property type="entry name" value="SHC-transforming protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051235">
        <property type="entry name" value="CEP152/SHC-Transforming"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006019">
        <property type="entry name" value="PID_Shc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035676">
        <property type="entry name" value="SHC_SH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337">
        <property type="entry name" value="SHC TRANSFORMING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337:SF4">
        <property type="entry name" value="SHC-TRANSFORMING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00629">
        <property type="entry name" value="SHCPIDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="PID" evidence="3">
        <location>
            <begin position="171"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="4">
        <location>
            <begin position="538"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="633" mass="69675" checksum="72AAD3E4C5252A8F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5L5</accession>
    <name>A0A452J5L5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J5L5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035773"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040500.1">
        <property type="protein sequence ID" value="ENSGAGP00000035773.1"/>
        <property type="gene ID" value="ENSGAGG00000025390.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01479">
        <property type="entry name" value="Sec24-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000004">
        <property type="entry name" value="SEC24 homolog B, COPII coat complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000019">
        <property type="entry name" value="SEC24 homolog B, COPII coat complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041742">
        <property type="entry name" value="Sec24-like_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF42">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC24B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="16">
        <location>
            <begin position="513"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="17">
        <location>
            <begin position="586"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="19">
        <location>
            <begin position="830"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="18">
        <location>
            <begin position="925"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="15">
        <location>
            <begin position="1052"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="255"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="294"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="310"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="350"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1179" mass="129510" checksum="2F000AC00571EAB4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5R1</accession>
    <name>A0A452J5R1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">procollagen-proline 3-dioxygenase</fullName>
            <ecNumber evidence="4">1.14.11.7</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the leprecan family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.7" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452J5R1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035694"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040416.1">
        <property type="protein sequence ID" value="ENSGAGP00000035694.1"/>
        <property type="gene ID" value="ENSGAGG00000025337.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019797">
        <property type="term" value="F:procollagen-proline 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032963">
        <property type="term" value="P:collagen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000003">
        <property type="entry name" value="Prolyl 3-hydroxylase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056585">
        <property type="entry name" value="Leprecan_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039575">
        <property type="entry name" value="P3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14049">
        <property type="entry name" value="LEPRECAN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14049:SF1">
        <property type="entry name" value="PROLYL 3-HYDROXYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23557">
        <property type="entry name" value="TPR_leprecan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="10" id="KW-0847">Vitamin C</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019314622" description="procollagen-proline 3-dioxygenase" evidence="17">
        <location>
            <begin position="16"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="18">
        <location>
            <begin position="529"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="385"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="690" mass="78283" checksum="341A119F5EB2BC97" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5Y2</accession>
    <name>A0A452J5Y2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RecQ-mediated genome instability protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates. It is required to regulate sister chromatid segregation and to limit DNA crossover. Essential for the stability, localization, and function of BLM, TOP3A, and complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RMI2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J5Y2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035878"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040619.1">
        <property type="protein sequence ID" value="ENSGAGP00000035878.1"/>
        <property type="gene ID" value="ENSGAGG00000025458.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043007">
        <property type="term" value="P:maintenance of rDNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000042">
        <property type="term" value="P:negative regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033045">
        <property type="term" value="P:regulation of sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000224">
        <property type="entry name" value="RecQ mediated genome instability 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032245">
        <property type="entry name" value="RMI2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33962:SF1">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33962">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 2 RMI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16100">
        <property type="entry name" value="RMI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00055932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="139" mass="14894" checksum="91773F767C6FA0E3" modified="2019-05-08" version="1">MAGEPRSPPVKVLAAQLRQCRRAAGGPWLLAREEAGRGPLAVPVVWMQGTVLAVEAGGARGGSARLQDESGPFTVLGVERVPKGRPCLSAGKYVMVMGVVHSCSPEPILQAVKMTDLSDNPIHRSMWSFEVEDLHRNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFT7</accession>
    <name>A0A452GFT7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyridoxal kinase</fullName>
            <ecNumber evidence="9">2.7.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Pyridoxine kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively. PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>pyridoxal + ATP = pyridoxal 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10224"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17310"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:10225"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>pyridoxamine + ATP = pyridoxamine 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57761"/>
            <dbReference type="ChEBI" id="CHEBI:58451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:25105"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>pyridoxine + ATP = pyridoxine 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25108"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16709"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:25109"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxal: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxamine 5'-phosphate from pyridoxamine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxine 5'-phosphate from pyridoxine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452GFT7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000415"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000467.1">
        <property type="protein sequence ID" value="ENSGAGP00000000415.1"/>
        <property type="gene ID" value="ENSGAGG00000000363.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00298"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000007">
        <property type="entry name" value="Pyridoxal kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00687">
        <property type="entry name" value="pyridox_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0007">Acetylation</keyword>
    <keyword evidence="17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0915">Sodium</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="26">
        <location>
            <begin position="205"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00018134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="424" mass="46067" checksum="0AE0853532BDC4FE" modified="2019-05-08" version="1">MGGSGSVGAVSEGLWGAVGVWVQRECGGGRGPFKWRCRALPAHSGSGLADVKAFRCLRRSPESAAWARRFALPCAPGGSALPGEGRLRLRLRPAACSCGSGGRAGRAGGMEAEEARCRVLSIQSHVVCGYVGNKAASFPLQVLGFEVDPVNSVQFSNHTGYAHWKGQMLNSDELHELYEGLKLNNVNHYDYVLTGYTRDASFLATVVDIVQELKLQNSDLVYVCDPVMGDKWKGEGSMYVPKELLPVYRDRVVPVADIITPNQFEAELLTGRKIHTEKEALEVMDMLHAMGPETVVITSSDLQGSLGSDFLIALGSQRKTRADGTKVTQRIRMESPKVDADFVGTGDLFAAMLLAWTHKHPNNLKVACEKTVSAMQHVLQRTIKCAKAHAGKGNKPSPAQLELRMVQSKKDIENPEIIIKATEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GG11</accession>
    <name>A0A452GG11_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GG11"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000482"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000541.1">
        <property type="protein sequence ID" value="ENSGAGP00000000482.1"/>
        <property type="gene ID" value="ENSGAGG00000000367.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000848">
        <property type="entry name" value="Zinc finger protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000367">
        <property type="entry name" value="Zinc finger protein 572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000176">
        <property type="entry name" value="zinc finger protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF162">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="279"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="384"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="414" mass="45073" checksum="CD9F9A36823E81A2" modified="2019-05-08" version="1">ASSDRPSLALAKATGLSSPQAPTAPPAGLCSPPPLPAVSPLRARSPRWVPGPGDLFGSSPGIDSDVDCLFIPAVDRTLSKEGANTVRTFHFAPSAHSSLCPFPLWENLADSTGCGDEVLRDSTANPQQDKKPIWCNKEEEFGAPSPAPQLDVLGDLTSAPQPAEKPFRCSECDKTFGQSSHLLQHQITHTGDRPYQCPDCGKRFSWSSALCQHQRIHRGERPYQCPDCGKRFAQSSNLTTHRRTHTGEKPYRCPQCGRGFSQNANLLTHQRVHAGVKRHECPQCGRVFVERSSLLKHQGVHLAEKPYQCPECGRSFTQGSTLAQHLRIHTGEKPYQCGECGKCFGQSSALAQHRRAHTGERSYHCHQCGKAFSQSSNLITHQAPAVSPLRARSPRWVPGPGDLFGSSPGRGACQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GG56</accession>
    <name>A0A452GG56_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pre-rRNA-processing protein TSR1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Required during maturation of the 40S ribosomal subunit in the nucleolus.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GG56"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000564"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000633.1">
        <property type="protein sequence ID" value="ENSGAGP00000000564.1"/>
        <property type="gene ID" value="ENSGAGG00000000465.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF1">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome biogenesis protein BMS1/TSR1 C-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="177" mass="20657" checksum="CF735F25A898C190" modified="2019-05-08" version="1">MSVLNSVVRRHLGNNETVKAKEELIFHCGFRRFRASPLYSQHSSADKHKLERLLSNGMHDLIATGFLLSVDPDKIIIKRLVLSGHPLKIFSKTAVVQYMFFNREDVLWFKPVQLRTKWGHRGHITELLGTHGHMKCHFDGQLKSQDTVLLNLYKHVFPKWTYDPHVPEPVPWVRNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGQ9</accession>
    <name>A0A452GGQ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">One cut domain family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CUT homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GGQ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000762"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000860.1">
        <property type="protein sequence ID" value="ENSGAGP00000000762.1"/>
        <property type="gene ID" value="ENSGAGG00000000645.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000054">
        <property type="entry name" value="One cut domain family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000005">
        <property type="entry name" value="One cut domain family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003350">
        <property type="entry name" value="CUT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051649">
        <property type="entry name" value="CUT_Homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14057:SF50">
        <property type="entry name" value="ONE CUT DOMAIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14057">
        <property type="entry name" value="TRANSCRIPTION FACTOR ONECUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02376">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01109">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51042">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CUT" evidence="13">
        <location>
            <begin position="226"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="327"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="329"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="410" mass="45943" checksum="823048DED38DB62C" modified="2019-05-08" version="1">MNVQLGLENAGDMSSQDQELMHSQGRALHSALHRPSLVASVLDGSEYHRAEHHLATSLHPGLGFGGDSPTGATNSYTTLTPLQPLHEKFHHQHHHHQCLPVGNVIGSFTLMREERGLGSGGNFYSPYHKDLGMGQSLSSLPSPNLTPMHSYGHSAGHLGNEKMVAANNFEAHPAMFGRIDQHFSRELSPSQNSTMGSPNSMGQHPYGHARPDMNSRPNQPISTQTTVLSSQLEEINTKEVAQRIIAELKRYSIPQAIFAERVLCRSQGTLSDLLRNPKPWSKLKSGRETFKRMWRWLQEPEFQRMAALRLEACKRKEQEQSKADRNHVPKRHRLVFTDIQRRTLHAIFHENQRPSKDLQVTIAQQLGLELSTVSNFFMNARRRSLDKWLEEGRPPASGAHAASAVTCTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIP4</accession>
    <name>A0A452GIP4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein 255A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Protein FAM70A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM255 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GIP4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001759.1">
        <property type="protein sequence ID" value="ENSGAGP00000001543.1"/>
        <property type="gene ID" value="ENSGAGG00000001248.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028014">
        <property type="entry name" value="TMEM255"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33721:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 255A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33721">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 255B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14967">
        <property type="entry name" value="FAM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="323" mass="35483" checksum="DB661EF0C37A686B" modified="2019-05-08" version="1">MRQSLTQQRSSAVPLPDSAGSFNRRKRNSLYVTVTLLIVSVLILTVGLAATTRTQNVTVGGYYPGVILGFGSFLGIIGSNLIENKRQMLVASIVFISFGVIAAFCCAIVDGVFAARHIDLRPLYAGRCQYYSKSTTPPEGICNPQRRGPCTPKIKTNTCFCCDLYNCGNRVEISGGYYEYIDVSSCQDIIHLYHLLWSATILNIVGLFLGIITAAILGGFKDMTPSLPTLNCTVENAHPSVSYYSRPQVTSYNTYYHSTPHLPPYSAYDFQHSSVFPASTPSGLSDDPQSMSPSPSYMWSSNAPPRYSPPYFPPFEKPPPYTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLY8</accession>
    <name>A0A452GLY8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GLY8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002697"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003091.1">
        <property type="protein sequence ID" value="ENSGAGP00000002697.1"/>
        <property type="gene ID" value="ENSGAGG00000002163.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007616">
        <property type="term" value="P:long-term memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF158">
        <property type="entry name" value="PLATELET GLYCOPROTEIN IB ALPHA CHAIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019120392" description="LRRCT domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="6">
        <location>
            <begin position="216"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="249"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="309" mass="33181" checksum="AF876102C239F82F" modified="2019-05-08" version="1">MPCDVCVLCMLLSPLQTMLCPTSPHLVNCSGQGLSVVPLALPRNTGILLLSTNRLASVSTASFQHLPELAELDLSGNVLGVLHTVAPLLLLQELILSHNALEVLPTLQGLPALTRLTLAHNNISELLPGAFRDVGKLTELNLRGNQLRTLPEEAFEGLAGLKDLDLSDNALEELPQGLLAGLELNTLWLSGNQLRTLPSGFFPEGHMFAYVYLAGNPWRCDCGLAYLQEWIVDNVISIYIQEQKSDGLLTQNDPQSPVCDSPLRHHGKAGDTDLDIDGTMTEEGTPVPSTTVPSTTLPPTTPVPPTTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GM83</accession>
    <name>A0A452GM83_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-crystallin B2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Beta-B2 crystallin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Beta-crystallin Bp</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homo/heterodimer, or complexes of higher-order. The structure of beta-crystallin oligomers seems to be stabilized through interactions between the N-terminal arms.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GM83"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002939"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003366.1">
        <property type="protein sequence ID" value="ENSGAGP00000002939.1"/>
        <property type="gene ID" value="ENSGAGG00000002335.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000005">
        <property type="entry name" value="Crystallin, beta B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF11">
        <property type="entry name" value="BETA-CRYSTALLIN B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="10">
        <location>
            <begin position="61"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="10">
        <location>
            <begin position="111"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="10">
        <location>
            <begin position="153"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="209" mass="24118" checksum="21359BDA47708D27" modified="2019-05-08" version="1">MVKTLVSCSCCERFLTVNPITSLSLIFSCFAFPKITVFSQCMQKFLVPCPSISNVCTLFFSSWVGYEQANCKGEQFVFEKGEYPRWDSWTNSRKSDTIGALRPIKVDSQEHKIVLYENPSFTGKKIEIIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGYQYLFEKGDYKDSSEFGAQHPQIQSVRRIRDMQWHQRGAFHPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNG9</accession>
    <name>A0A452GNG9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Mitochondrial import receptor subunit TOM34</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Translocase of outer membrane 34 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Plays a role in the import of cytosolically synthesized preproteins into mitochondria. Binds the mature portion of precursor proteins. Interacts with cellular components, and possesses weak ATPase activity. May be a chaperone-like protein that helps to keep newly synthesized precursors in an unfolded import compatible state.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with HSP90A, VCP, ATP6V1D, KIAA0665, AMPK, and DMAP1 through its TPR repeat.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the Tom34 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GNG9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003403"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003924.1">
        <property type="protein sequence ID" value="ENSGAGP00000003403.1"/>
        <property type="gene ID" value="ENSGAGG00000002721.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000221">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000312">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051982">
        <property type="entry name" value="CiliaryAsmbly_MitoImport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45984:SF2">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45984">
        <property type="entry name" value="RNA (RNA) POLYMERASE II ASSOCIATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 18" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="repeat" description="TPR" evidence="18">
        <location>
            <begin position="193"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="18">
        <location>
            <begin position="227"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="18">
        <location>
            <begin position="261"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00075963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="309" mass="34270" checksum="73EB225D6BFE2F5F" modified="2019-05-08" version="1">MAPAAARVSELRRAGNEQFRSGQYCQAAALYSQALGLLEAAGTGRSGPEASVLYSNRAACYLKDGNCSLCVKDCSAALDLVPFGIKPLLRRAAAYEALERYQLAYVDYKTVLQVDCTVQAAHDGVNRMTKALLEKDGLDWRQKLPPIPSVPISAQRRWDFPSAGSQVAAPRHKATETGSLEKQVPTAAMIERAKTLKQEGNELVKKGNHKKAIEKYSESLKLSNQECATYTNRALCYLSLKQYKEAIQDCTEALELDAKNIKAFYRRAQAFKELKDYKSSMTDINSLLKIEPKNIAAQKLLQELNKNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GP14</accession>
    <name>A0A452GP14_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000003411"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003934.1">
        <property type="protein sequence ID" value="ENSGAGP00000003411.1"/>
        <property type="gene ID" value="ENSGAGG00000002759.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="63" mass="7632" checksum="E54E877288A0A6C2" modified="2019-05-08" version="1">MLVWXXXXKGTLLHCWWECKLVQPLWKTVWRFLKELKVELPFDPAIPLLGIYPEEKKSLYEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPJ7</accession>
    <name>A0A452GPJ7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Activating signal cointegrator 1 complex subunit 3</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">DEAH-box protein 16</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Required for pre-mRNA splicing as a component of the spliceosome. Contributes to pre-mRNA splicing after spliceosome formation and prior to the first transesterification reaction. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. Also plays a role in innate antiviral response by acting as a pattern recognition receptor sensing splicing signals in viral RNA. Mechanistically, TRIM6 promotes the interaction between unanchored 'Lys-48'-polyubiquitin chains and DHX16, leading to DHX16 interaction with RIGI and ssRNA to amplify RIGI-dependent innate antiviral immune responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">Component of pre-catalytic spliceosome complexes. Component of the minor spliceosome, which splices U12-type introns. Interacts with GPKOW. Interacts with TRIM6. Interacts with RIGI.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the DEAD box helicase family. DEAH subfamily. DDX16/PRP8 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452GPJ7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003828"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004434.1">
        <property type="protein sequence ID" value="ENSGAGP00000003828.1"/>
        <property type="gene ID" value="ENSGAGG00000003115.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17974">
        <property type="entry name" value="DEXHc_DHX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000818">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF83">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE DHX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0347">Helicase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="15" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="24">
        <location>
            <begin position="325"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="25">
        <location>
            <begin position="514"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="76"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00062452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00069571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="957" mass="110525" checksum="32C5FDC9842BFC48" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPU4</accession>
    <name>A0A452GPU4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPU4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003841"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004451.1">
        <property type="protein sequence ID" value="ENSGAGP00000003841.1"/>
        <property type="gene ID" value="ENSGAGG00000003130.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13954">
        <property type="entry name" value="7tmA_OR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="195"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="314" mass="34979" checksum="11F4297971E6C7C1" modified="2019-05-08" version="1">MTNSTAGVEFLLLGFHSLPGWQTLLFFVFLVIYILTITGNIVIISVVRLEPRLHSPMYSFLQNLSFLEICYTSTIMPKMLANLLVERKAISFAGCMAQLYCFVFLGATECFLLAVMAYDRYLAICYPLHYTVAMSDASCTLLATVSWVTGIFTGLLPCILISRLPFCGSNQIKHFFCDIPPLLKLSCSDTSTTEVIIFILSLLVLVSCLLLTLVSYLFIILTILKIPSSSGKRKTFSTCGSHLAVVAIYYGTMISMYVRPTSSLPSELNKIVSVCYTIITPLLNPVIYSLRNKDFSDALKKVISRQCSLTNKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQS6</accession>
    <name>A0A452GQS6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphomannomutase</fullName>
            <ecNumber evidence="14">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 14">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 14">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A452GQS6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004195"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004916.1">
        <property type="protein sequence ID" value="ENSGAGP00000004195.1"/>
        <property type="gene ID" value="ENSGAGG00000003474.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061728">
        <property type="term" value="P:GDP-mannose biosynthetic process from mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02585">
        <property type="entry name" value="HAD_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1240.20:FF:000001">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000216">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005002">
        <property type="entry name" value="PMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043169">
        <property type="entry name" value="PMM_cap"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466:SF2">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03332">
        <property type="entry name" value="PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00445">
        <property type="entry name" value="alpha-phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01143">
        <property type="entry name" value="C2.B.3:_Phosphomannomutase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="247" mass="27951" checksum="20D25D2CA276194B" modified="2019-05-08" version="1">MAAPGSSVLCLFDVDGTLTAPRQKITAEMADFLQKLRKKVRVGVVGGSDFDKVQEQLGDDVVEKFDYVFPENGLVAYKDGKFLCKQSIQSHLGEDLLQDLINYCLSYIAKIKLPKKRGTFIEFRNGMLNVSPVGRSCSQEERIEFYELDKREHIREKFVADLQREFAGKGLTFSIGGQISFDVFPNGWDKTYCLGIACNDGYKTIYFFGDKTMPGGNDYEIYTDSRTVGHSVTSPEDTRRICEELFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GU47</accession>
    <name>A0A452GU47_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452GU47"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005584"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006502.1">
        <property type="protein sequence ID" value="ENSGAGP00000005584.1"/>
        <property type="gene ID" value="ENSGAGG00000004529.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16810">
        <property type="entry name" value="RING_CH-C4HC3_MARCH11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047906">
        <property type="entry name" value="MARCHF11_RING_CH-C4HC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046356">
        <property type="entry name" value="MARCHF4/9/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46053">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46053:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="15">
        <location>
            <begin position="37"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="277" mass="30992" checksum="25383D1F306C515F" modified="2019-05-08" version="1">EGRSGGSERARGQAGAAGGDKPETGSVCGSDSGSDSNPGGAGPICKICFQGAEQGELLNPCRCDGSVRYTHQLCLLKWISERGSWTCELCCYRYHVIAIKMKKPCQWQRITITLVEKVQMIAVILGSLFLVASVTWLLWSAFSPYAVWQRKDILFQICYGMYGFMDLVCIGLIVHEGASVYRVFKRWRAVNLHWDVLNYDKATDIEESNRGASSPGRTLWLPLTAFRNRNLIHPTQLTSPRFQCGYVLLHLFNRMRPHEDSSEDNSSGEVVMRVTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUV6</accession>
    <name>A0A452GUV6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">IMD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GUV6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005896"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006876.1">
        <property type="protein sequence ID" value="ENSGAGP00000005896.1"/>
        <property type="gene ID" value="ENSGAGG00000004797.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22060">
        <property type="entry name" value="WH2_MTSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030127">
        <property type="entry name" value="MTSS1/MTSS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708">
        <property type="entry name" value="ACTIN BUNDLING/MISSING IN METASTASIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708:SF8">
        <property type="entry name" value="PROTEIN MTSS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08397">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51338">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMD" evidence="2">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="569"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="337"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="572"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="618" mass="66769" checksum="B21EF338F162EDD5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVU1</accession>
    <name>A0A452GVU1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Plexin-A4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Coreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with NRP1 and NRP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000006155"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007164.1">
        <property type="protein sequence ID" value="ENSGAGP00000006155.1"/>
        <property type="gene ID" value="ENSGAGG00000004151.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021785">
        <property type="term" value="P:branchiomotor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021612">
        <property type="term" value="P:facial nerve structural organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050919">
        <property type="term" value="P:negative chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048841">
        <property type="term" value="P:regulation of axon extension involved in axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050920">
        <property type="term" value="P:regulation of chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021537">
        <property type="term" value="P:telencephalon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021637">
        <property type="term" value="P:trigeminal nerve structural organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01180">
        <property type="entry name" value="IPT_plexin_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01179">
        <property type="entry name" value="IPT_plexin_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01181">
        <property type="entry name" value="IPT_plexin_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12790">
        <property type="entry name" value="RasGAP_plexin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000005">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000006">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000123">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000071">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000339">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000018">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000032">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000329">
        <property type="entry name" value="Plexin A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001973">
        <property type="entry name" value="Plexin A4, B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013548">
        <property type="entry name" value="Plexin_cytoplasmic_RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046800">
        <property type="entry name" value="Plexin_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041019">
        <property type="entry name" value="TIG1_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041362">
        <property type="entry name" value="TIG2_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF34">
        <property type="entry name" value="PLEXIN-A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08337">
        <property type="entry name" value="Plexin_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20170">
        <property type="entry name" value="Plexin_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24479">
        <property type="entry name" value="PSI_PlexinA-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18020">
        <property type="entry name" value="TIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17960">
        <property type="entry name" value="TIG_plexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="895"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="18">
        <location>
            <begin position="156"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="18">
        <location>
            <begin position="311"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="18">
        <location>
            <begin position="459"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="19">
        <location>
            <begin position="513"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="19">
        <location>
            <begin position="609"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="19">
        <location>
            <begin position="696"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="19">
        <location>
            <begin position="798"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="921"/>
            <end position="948"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1567" mass="175325" checksum="40A62887F07C0AFC" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GW66</accession>
    <name>A0A452GW66_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Large ribosomal subunit protein uL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">60S ribosomal protein L3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the large ribosomal subunit. Interacts with DHX33.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GW66"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006315"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007340.1">
        <property type="protein sequence ID" value="ENSGAGP00000006315.1"/>
        <property type="gene ID" value="ENSGAGG00000005088.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000079">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1430.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000004">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000351">
        <property type="entry name" value="Ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 17" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9 17" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="403" mass="46209" checksum="B5BCA1D575427952" modified="2019-05-08" version="1">MSHRKFSAPRHGSLGFLPRKRSRRHRGKVKSFPKDDPSKPIHLTAFLGYKSGMTHIVREVDRPGSKVNKKEVVEAVTVVETPPMVIVGIVGYVQTPRGLRTFKTIFAEHISDECKRRFYKNWHKSKKKAFTKYCKKWQDEEGKKQLEKDFNSMKKYCQVIRVIAHTQMRLLPLRQKKSHLMEIQVNGGTIAEKVDWAREKLEQQVPVSTVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHRGLRKVACIGAWHPARVAFSVARAGQKGYHHRTEINKKVYKIGQGYQVKDGKLIKNNASTDYDLSDKSINPLGGFVHYGEVTNDFIMLKGCVVGTKKRVLTLRKSLLVQTKRRALEKIDLKFIDTTSKFGHGRFQTVEEKKAFMGPLKKDRIAKEETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWI2</accession>
    <name>A0A452GWI2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MATH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GWI2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006451"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007508.1">
        <property type="protein sequence ID" value="ENSGAGP00000006451.1"/>
        <property type="gene ID" value="ENSGAGG00000005221.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000001">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049342">
        <property type="entry name" value="TRAF1-6_MATH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF96">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21355">
        <property type="entry name" value="TRAF-mep_MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="MATH" evidence="6">
        <location>
            <begin position="112"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="263" mass="29226" checksum="1ED0A6860A29B1A7" modified="2019-05-08" version="1">MARILYLEEKVSARPTTRGKEVRTPLASSPLTSDLSRLAPAPATLSCLLPCGILRPTHEHPSHRLDPRTQLPPTSPSPSFQVAQQDTLLALKDVMISNLGARVEALEQTSYNGQFLWRLPDIGRKMQQALSRQTPALTSPSFYTGRYGYKLCLKVYLNGDGTGAGTHISLFLVLMRGEYDCWLKWPFHHKVTFTLLDQVGERHVSSSFRPSEASSSFQRPVSNCNIASGVPEFFPLCQLQAPEATYIHEDTLAFKVLIGDTNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWN1</accession>
    <name>A0A452GWN1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">A disintegrin and metalloproteinase with thrombospondin motifs 4</fullName>
            <ecNumber evidence="17">3.4.24.82</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Aggrecanase-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Glutamyl endopeptidase. Bonds cleaved include 370-Thr-Glu-Gly-Glu-|-Ala-Arg-Gly-Ser-377 in the interglobular domain of mammalian aggrecan.</text>
            <dbReference type="EC" id="3.4.24.82"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="21">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with SRPX2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.82" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A452GWN1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006513"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007576.1">
        <property type="protein sequence ID" value="ENSGAGP00000006513.1"/>
        <property type="gene ID" value="ENSGAGG00000005272.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04273">
        <property type="entry name" value="ZnMc_ADAMTS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000006">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.60:FF:000003">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF38">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="21" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="13 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 21 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 24" id="KW-0732">Signal</keyword>
    <keyword evidence="10 21 23" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019270096" description="A disintegrin and metalloproteinase with thrombospondin motifs 4" evidence="24">
        <location>
            <begin position="25"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="25">
        <location>
            <begin position="214"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="active site" evidence="20 23">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="21">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 23">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 23">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 23">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="289"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="318"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="335"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="373"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="445"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="463"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="491"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="528"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="532"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="543"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00040099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="834" mass="90720" checksum="D932CED955D2987B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWR6</accession>
    <name>A0A452GWR6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GWR6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006561"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007634.1">
        <property type="protein sequence ID" value="ENSGAGP00000006561.1"/>
        <property type="gene ID" value="ENSGAGG00000005313.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057428">
        <property type="entry name" value="EFHB_EF-hand_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040193">
        <property type="entry name" value="EFHC1/EFHC2/EFHB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086">
        <property type="entry name" value="EF-HAND DOMAIN C-TERMINAL CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086:SF12">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING FAMILY MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25325">
        <property type="entry name" value="EF-hand_EFHB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="320"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="356"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="403"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="572" mass="64866" checksum="D3B422B56D1708AB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXS1</accession>
    <name>A0A452GXS1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Acrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Golgi apparatus membrane</location>
            <topology evidence="13">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXS1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006951"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008058.1">
        <property type="protein sequence ID" value="ENSGAGP00000006951.1"/>
        <property type="gene ID" value="ENSGAGG00000005606.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01866">
        <property type="entry name" value="Rab2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000275">
        <property type="entry name" value="Putative ras-related protein Rab-2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="212" mass="23524" checksum="4792FB3FC97F46A1" modified="2019-05-08" version="1">MAYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNKSDLESRREVKKEEGESFAREHGLIFMETSAKTASNVEEAFINTAKEIYEKIQEGVFDINNEANGIKIGPQHAASNATLAGNQGGQQAGGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXT1</accession>
    <name>A0A452GXT1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXT1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006961"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008068.1">
        <property type="protein sequence ID" value="ENSGAGP00000006961.1"/>
        <property type="gene ID" value="ENSGAGG00000005612.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017993">
        <property type="entry name" value="Atrophin-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002951">
        <property type="entry name" value="Atrophin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859:SF9">
        <property type="entry name" value="ATROPHIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859">
        <property type="entry name" value="ATROPHIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03154">
        <property type="entry name" value="Atrophin-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01222">
        <property type="entry name" value="ATROPHIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="573"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="751"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="278"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="434"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="461"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="504"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="602"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="615"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="685"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="751"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1145" mass="121345" checksum="8A09CFE76701BE59" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZ34</accession>
    <name>A0A452GZ34_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Tyrosine-protein kinase receptor</fullName>
            <ecNumber evidence="20">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Receptor with tyrosine-protein kinase activity. Functions as a pH sensing receptor which is activated by increased extracellular pH. Activates an intracellular signaling pathway that involves IRS1 and AKT1/PKB.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 20">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Probable tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chains carry the kinase domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A452GZ34"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007418"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008568.1">
        <property type="protein sequence ID" value="ENSGAGP00000007418.1"/>
        <property type="gene ID" value="ENSGAGG00000005944.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005899">
        <property type="term" value="C:insulin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005009">
        <property type="term" value="F:insulin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043560">
        <property type="term" value="F:insulin receptor substrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05032">
        <property type="entry name" value="PTKc_InsR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000050">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000014">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000087">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000108">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000026">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.20.20:FF:000001">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.20.20">
        <property type="entry name" value="Receptor L-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006211">
        <property type="entry name" value="Furin-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000494">
        <property type="entry name" value="Rcpt_L-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036941">
        <property type="entry name" value="Rcpt_L-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002011">
        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF338">
        <property type="entry name" value="INSULIN RECEPTOR-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00757">
        <property type="entry name" value="Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01030">
        <property type="entry name" value="Recep_L_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00239">
        <property type="entry name" value="RECEPTOR_TYR_KIN_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 20" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 22" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019372864" description="Tyrosine-protein kinase receptor" evidence="22">
        <location>
            <begin position="36"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="302"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="642"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="804"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="554"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="838"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00065424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1153" mass="129819" checksum="08166016F8B278CF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZG3</accession>
    <name>A0A452GZG3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Synaptic cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZG3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007666"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008848.1">
        <property type="protein sequence ID" value="ENSGAGP00000007666.1"/>
        <property type="gene ID" value="ENSGAGG00000006136.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000006">
        <property type="entry name" value="Cholinergic receptor nicotinic beta 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000008">
        <property type="entry name" value="Cholinergic receptor nicotinic beta 4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000034">
        <property type="entry name" value="Cholinergic receptor nicotinic beta 4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="458"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="26"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="238"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="489" mass="55985" checksum="3C5F55E5F7EEEF55" modified="2019-05-08" version="1">MVFTAYNCSAMTMFLEILGINAADAEEKLMNHLLSPARYNKLIRPAVNSSQLVSIELQVSLAQLISVNEREQIMSTNVWLNQEWTDYRLAWQPSDYDGINKLRIPSKHIWLPDIVLYNNADGTYEVSLYTNAIVNNNGSIFWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDMVLKTSMARMDDFTPSGEWDIVALPGRRTVNPLDPKYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLIGKYLMFTMVLVTFSIVTSVCVLNVHHRSPSTHTMPPWVKVVFLQKLPTFLFMKRPENNSARQRLSKRRKNKSDHLCTDPADLYKNSTYFVNTASARKYDLKVTENLDNISGHQDFRLRSSMKFSPEIQETIDGVSFIAGHMKNEDNDQSVIEDWKYVAMVVDRLFLWIFIFVCVLGTVGLFLQPLFQNHSAAASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H054</accession>
    <name>A0A452H054_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H054"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007730"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008917.1">
        <property type="protein sequence ID" value="ENSGAGP00000007730.1"/>
        <property type="gene ID" value="ENSGAGG00000006176.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05512">
        <property type="entry name" value="Bromo_brd1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15572">
        <property type="entry name" value="PHD_BRPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20158">
        <property type="entry name" value="PWWP_BRPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000008">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000008">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000007">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000007">
        <property type="entry name" value="Bromodomain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF19">
        <property type="entry name" value="BROMODOMAIN AND PHD FINGER-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 11" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="16">
        <location>
            <begin position="212"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="18">
        <location>
            <begin position="266"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="15">
        <location>
            <begin position="585"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="PWWP" evidence="17">
        <location>
            <begin position="1043"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="74"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="386"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="760"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="912"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="544"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="402"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="775"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1172" mass="132320" checksum="155F24CDF0DEB11C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0I7</accession>
    <name>A0A452H0I7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H0I7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007867"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009068.1">
        <property type="protein sequence ID" value="ENSGAGP00000007867.1"/>
        <property type="gene ID" value="ENSGAGG00000006267.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="102" mass="11892" checksum="D7445026B9154D30" modified="2019-05-08" version="1">MSGTSSPEAVKKLLENTQGDLRGLSLECRKKFPPVKEVRDSVKTRTHSLRTTAVIEWKKHEFAIEVDIQTTPCVFLHFWLCYVMVSKSYLYLSRFTLYTEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0U0</accession>
    <name>A0A452H0U0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H0U0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008115"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009346.1">
        <property type="protein sequence ID" value="ENSGAGP00000008115.1"/>
        <property type="gene ID" value="ENSGAGG00000006445.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22030">
        <property type="entry name" value="HMG-box_SoxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000003">
        <property type="entry name" value="Putative transcription factor SOX-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051356">
        <property type="entry name" value="SOX/SOX-like_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45789">
        <property type="entry name" value="FI18025P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45789:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="8">
        <location>
            <begin position="418"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="5">
        <location>
            <begin position="418"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="258"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="573"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="177"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="317"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="370"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="575"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="617" mass="69556" checksum="5F74F2D90094ED8E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H407</accession>
    <name>A0A452H407_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ELMO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000009326"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010714.1">
        <property type="protein sequence ID" value="ENSGAGP00000009326.1"/>
        <property type="gene ID" value="ENSGAGG00000007376.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006816">
        <property type="entry name" value="ELMO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050868">
        <property type="entry name" value="ELMO_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771:SF2">
        <property type="entry name" value="ELMO DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771">
        <property type="entry name" value="ENGULFMENT AND CELL MOTILITY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04727">
        <property type="entry name" value="ELMO_CED12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51335">
        <property type="entry name" value="ELMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELMO" evidence="1">
        <location>
            <begin position="158"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="376" mass="41762" checksum="9E8D7BE416B3DCF4" modified="2019-05-08" version="1">MNRCTADTNQSEEQGIFLPPAVKSVPISVLKQSGLLQALAEGPNGCTEADPSAEVLQAQEEWEAVESLQSGLVGTSAGQVPLIAFNEALQYFQTADLSECRKKIQPTVQRRGLSAVAXGLSAVAHFFFGPPRLHQQLQGERDLVLAIAQCGLDNNEKVHMRILQTIYRKLTGSRFDCPRYGAHWEELGFQGMDPGTDLRGTGLLGLMQILYFVMDSRTLPLARDVFKLSHHESQNFPFCVMSINITRIVIQALREEHLSRECNRRQQVIAVLNDLYVATSLRLYVIWKTQQKTISDSGFVLKELEAFAKKKPKQLLRYLEIYMSGRAVSVIDGGFQHPSSSSGVYLSSAISNSSKEINFTGVCDPQVEMEGEARLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H5V3</accession>
    <name>A0A452H5V3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thyrotropin-releasing hormone receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Thyroliberin receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor for thyrotropin-releasing hormone (TRH). Upon ligand binding, this G-protein-coupled receptor triggers activation of the phosphatidylinositol (IP3)-calcium-protein kinase C (PKC) pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H5V3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010045"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011528.1">
        <property type="protein sequence ID" value="ENSGAGP00000010045.1"/>
        <property type="gene ID" value="ENSGAGG00000007886.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000011529.1">
        <property type="protein sequence ID" value="ENSGAGP00000010046.1"/>
        <property type="gene ID" value="ENSGAGG00000007886.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004997">
        <property type="term" value="F:thyrotropin-releasing hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14995">
        <property type="entry name" value="7tmA_TRH-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002120">
        <property type="entry name" value="TRH_rcpt_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46061">
        <property type="entry name" value="THYROTROPIN-RELEASING HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46061:SF5">
        <property type="entry name" value="THYROTROPIN-RELEASING HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00751">
        <property type="entry name" value="THYROLIBRINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01846">
        <property type="entry name" value="TRHRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="45"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="364"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="410" mass="45271" checksum="621459DC6CD2B08D" modified="2019-05-08" version="1">MENSSKFDSPTNISLASSDAIFDVIEYKAISVFLVLVICGVGIVGNIMVVLVVLTTREMKTPTNCYLVSLAVADLMVLVAAGLPNISDSLAGTWIYGHAGCLGITYFQYLGINVSSCSITAFTVERYIAVCHPMRAHTMCTVSRAKRIIAFVWSCTSIYCMLWFFLVDINVNKSQHLECGYKVSRNLYLPIYLLDFALFFVTPLFVATILYGLIGRILFASPIRDQADAIAEPWRERSGKEKNGVDTRGSKPSSCSRTRGALSSRKQVTKMLAVVVILFALLWMPYRTLVLVNSFMDNPFLNPWFILFCRLCVYANSAINPVVYNLMSQKFRAAFKGLCKCGGEPTPHSLYMATASYSLAREPLPPTPQPLDHKEGKQPPVASNTALPKQQQPPPGSKGDNGDELYCSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H5V9</accession>
    <name>A0A452H5V9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H5V9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010033"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011516.1">
        <property type="protein sequence ID" value="ENSGAGP00000010033.1"/>
        <property type="gene ID" value="ENSGAGG00000007876.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF57">
        <property type="entry name" value="ENTH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="1">
        <location>
            <begin position="9"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="126" mass="14741" checksum="FC3CC3B611322B5C" modified="2019-05-08" version="1">MALRRHMKNFVKNYSDSEVKVREATSNDPWGPSSSLMLEISDLTYNTVSLLEIMNMIWHRMNDQGKNWRHVYKSLTLLDYLIKNGSKKVILHCREGLFNIQTLKDFHYIDEAGKDQGKCILPRPVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H5Z4</accession>
    <name>A0A452H5Z4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H5Z4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010093"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011583.1">
        <property type="protein sequence ID" value="ENSGAGP00000010093.1"/>
        <property type="gene ID" value="ENSGAGG00000007920.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000004">
        <property type="entry name" value="Keratin, type II cytoskeletal 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003054">
        <property type="entry name" value="Keratin_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616:SF21">
        <property type="entry name" value="KERATIN, TYPE II CYTOSKELETAL 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16208">
        <property type="entry name" value="Keratin_2_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01276">
        <property type="entry name" value="TYPE2KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="8">
        <location>
            <begin position="121"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="478"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="174"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="330"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="504" mass="54857" checksum="445D2F59BD9D3150" modified="2019-05-08" version="1">MSRSVRFSSSSAAPTGGFRQVRVSTVSSTRGLGGGAGLGRAGGFGSSSLYNLGSANKRISLGGGGSSYSVHSGYGFGSGYGAGVGGGFAPAGIQEVTINPSLLVPLNVQIDPTLQTVRKEEKEQIKTLNNKFASFIDKVRFLEQQNKVLETKWSLLQDQKTTRSNIAPMFEAYINNLRKQLDGWLNDKGRLEGELKNMQDLVEDFRNKYEDEINKRTAAENEFVILKKDVDASYMNKVELEAKVDAFTDEINFLRALHEAELRELQAQVSDTSVVLTMDNNRNLDMDSIIAEVKAQYEEMAKKSRMEAESWYQTKFETLQATAGKHGDELRNTKTEITEINRMIHRLQAEIDNVKSQRAKLEAAIAEAEERGEKAIKDAMAKQEELEAALNKAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLAGDHAGNVSVSVVNSSAGGGYSSAGGYGFGGGIGLGSGMGSGGLSFSSGGSPGGVKAYSLRTTSSSRRSDRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6B2</accession>
    <name>A0A452H6B2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H6B2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010203"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011711.1">
        <property type="protein sequence ID" value="ENSGAGP00000010203.1"/>
        <property type="gene ID" value="ENSGAGG00000007999.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="90"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="323" mass="36266" checksum="D3C0A13C59C83C4D" modified="2019-05-08" version="1">MEVKDNLGMAQHLNKYFASVFNKTSEEPCDDGGMINGNVDMEVDITATEVEAVLEQLDGTKSEGPDNLHPRILKELAREIASPLAKIFKQSVNSGVVPYDWRIANVVPIFKKGNKSDPGNYRPVSLTSVVCKVLEKILREKVVKDIEVNGNWDELQHGFTKGRSCQTNLISFFEKVTDYLDKGNAVDIIYLDFSKAFDTVPHGELLVKLEKMGMNMKVVSWIRNWLKGRLQRVVLKGELSGWKEVTSGVPQGSVLGPILFNLFITDLGTKSGNVLIKFADDTKLGGIANTEKDRDTIQEDLNHLVNWSNRNRMKYNSEKCKVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6N8</accession>
    <name>A0A452H6N8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H6N8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010375"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011911.1">
        <property type="protein sequence ID" value="ENSGAGP00000010375.1"/>
        <property type="gene ID" value="ENSGAGG00000008131.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="177" mass="20009" checksum="E6FA35296550118B" modified="2019-05-08" version="1">EELQIVCRKSLSPPRAYLYTDSWVVYKGLRAWVTAWEAKEWELAGRPLWGAGLWQQLLDYARQAPLAMRHVDAHTKAQTDEARWNAAADDLARGEVVQLAERYHEEGGHQGARATQYAALRQGTALPLSACRTARAGCPICSRLAPIALPEPDWQVDYICPLPPERRWQYHVSFSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H715</accession>
    <name>A0A452H715_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein-tyrosine sulfotransferase</fullName>
            <ecNumber evidence="13">2.8.2.20</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 13">Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-tyrosyl-[protein] + 3'-phosphoadenylyl sulfate = O-sulfo-L-tyrosine-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16801"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:11688"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:65286"/>
            <dbReference type="EC" id="2.8.2.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the protein sulfotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.20" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A452H715"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010483"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012034.1">
        <property type="protein sequence ID" value="ENSGAGP00000010483.1"/>
        <property type="gene ID" value="ENSGAGG00000008201.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000012036.1">
        <property type="protein sequence ID" value="ENSGAGP00000010485.1"/>
        <property type="gene ID" value="ENSGAGG00000008201.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008476">
        <property type="term" value="F:protein-tyrosine sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000290">
        <property type="entry name" value="Protein-tyrosine sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026634">
        <property type="entry name" value="TPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788">
        <property type="entry name" value="PROTEIN-TYROSINE SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788:SF6">
        <property type="entry name" value="PROTEIN-TYROSINE SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13469">
        <property type="entry name" value="Sulfotransfer_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604480" description="Protein-tyrosine sulfotransferase" evidence="14">
        <location>
            <begin position="26"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="375" mass="42280" checksum="701F84A885F4CF6B" modified="2019-05-08" version="1">MRVTMRRVLLGLGFAVALMVTVHLGQQMLECQELLGRGYGKRTHGLMRPENEELVMVDSSHIEYRYSKEMPLIFIGGVPRSGTTLMRAMLDAHPEVRCGEETRIIPRVLAMRQAWSKSGREKMRLDEAGVTDQVLDSAMQAFILEVIAKHGEPARYLCNKDPFTLKSSVYLSRLFPNSKFLLMVRDGRASVHSMITRKVTIAGFDLNSYRDCLTKWNKAIEVMYSQCAEIGQSRCLPVYYEQLVLHPEQSMRVIMQFLDISWSDAVLHHEELIGKPGGVSLSKIERSTDQVIKPVNLEALSKWIGHIPGDVLQDMAQIAPMLARLGYDPYANPPNYGNPDPLVINNTQRVMKGDFKTPANLKGHLQVTQNTSASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H857</accession>
    <name>A0A452H857_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone deacetylase 11</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with HDAC6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452H857"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010907"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012500.1">
        <property type="protein sequence ID" value="ENSGAGP00000010907.1"/>
        <property type="gene ID" value="ENSGAGG00000008475.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09993">
        <property type="entry name" value="HDAC_classIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000009">
        <property type="entry name" value="Histone deacetylase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044150">
        <property type="entry name" value="HDAC_classIV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF23">
        <property type="entry name" value="HISTONE DEACETYLASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="14">
        <location>
            <begin position="43"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="364" mass="41243" checksum="21148CB51EDB9585" modified="2019-05-08" version="1">MVLFFFSLRPHRTELYHEVPPTCWPIVYSPDYNITFMGLEKLHPFDAGKWGKVINFLKEEKLIADDLIVQAREATEEDLLVVHTRRYLNKLKWSFVVATITEIPPVLFLPNFLVQRKVLKPLRTQTGGTIMAGKLAIDRGWAINVGGGFHHCSSDKGGGFCAYADITLAIKFLFERVEGVSKATIIDLDAHQGNGHERDFMDDHKVYIMDVYNRYIYPGDGFAKRAIKRKIELDWGTEDTEYLQKVQTHVEGALNESRPDIIIYNAGTDILDGDPLGGLAISPQGIVKRDEVVFRAARNHRIPILMVTSGGYQKRTARIIADSILNLHNLRLIDKELATSEAKNPKVELMIRESILDLTNLSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8W8</accession>
    <name>A0A452H8W8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HORMA domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a key role in meiotic progression. Regulates 3 different functions during meiosis: ensures that sufficient numbers of processed DNA double-strand breaks (DSBs) are available for successful homology search by increasing the steady-state numbers of single-stranded DSB ends. Promotes synaptonemal-complex formation independently of its role in homology search. Plays a key role in the male mid-pachytene checkpoint and the female meiotic prophase checkpoint: required for efficient build-up of ATR activity on unsynapsed chromosome regions, a process believed to form the basis of meiotic silencing of unsynapsed chromatin (MSUC) and meiotic prophase quality control in both sexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with HORMAD2. Interacts with IHO1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H8W8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011021"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012619.1">
        <property type="protein sequence ID" value="ENSGAGP00000011021.1"/>
        <property type="gene ID" value="ENSGAGG00000008537.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048477">
        <property type="term" value="P:oogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051294">
        <property type="entry name" value="HORMA_MeioticProgression"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48225">
        <property type="entry name" value="HORMA DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48225:SF1">
        <property type="entry name" value="HORMA DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0469">Meiosis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0896">Oogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="HORMA" evidence="13">
        <location>
            <begin position="13"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="81" mass="9229" checksum="EE887D1D9350D02E" modified="2019-05-08" version="1">IAVIFPNKICTEQQSLILVKRLLAVAVSCITYLRGIFPECAYGTRYLDDLCVKILREDKNCPGSTQLVKWYVDFIAFITLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H912</accession>
    <name>A0A452H912_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PHD finger protein 20-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H912"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011065"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012669.1">
        <property type="protein sequence ID" value="ENSGAGP00000011065.1"/>
        <property type="gene ID" value="ENSGAGG00000007609.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044545">
        <property type="term" value="C:NSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20104">
        <property type="entry name" value="MBT_PHF20L1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15633">
        <property type="entry name" value="PHD_PHF20L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20454">
        <property type="entry name" value="Tudor_PHF20L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000049">
        <property type="entry name" value="PHD finger protein 20 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014002">
        <property type="entry name" value="Agenet_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040477">
        <property type="entry name" value="KDM4-like_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043449">
        <property type="entry name" value="PHF20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047405">
        <property type="entry name" value="Tudor_PHF20L1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15856:SF26">
        <property type="entry name" value="PHD FINGER PROTEIN 20-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15856">
        <property type="entry name" value="PHD FINGER PROTEIN 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16660">
        <property type="entry name" value="PHD20L1_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18104">
        <property type="entry name" value="Tudor_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00743">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Agenet" evidence="10">
        <location>
            <begin position="11"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="9">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Agenet" evidence="10">
        <location>
            <begin position="85"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="9">
        <location>
            <begin position="85"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="280"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="373"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="781"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="820"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="299"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="787"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="834"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="971" mass="110004" checksum="A67DCF718CDABFDB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9Y2</accession>
    <name>A0A452H9Y2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H9Y2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011448"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013107.1">
        <property type="protein sequence ID" value="ENSGAGP00000011448.1"/>
        <property type="gene ID" value="ENSGAGG00000008830.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007528">
        <property type="term" value="P:neuromuscular junction development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06716">
        <property type="entry name" value="PDZ2-PDZRN4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.370.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051971">
        <property type="entry name" value="E3_ubiquitin-PDZ_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545:SF5">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PDZRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545">
        <property type="entry name" value="PDZ DOMAIN CONTAINING RING FINGER PROTEIN 3, 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019510153" description="PDZ domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="132"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="246"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="443"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="507"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="373"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="443"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="525"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="762" mass="87798" checksum="A8C8C8275A134297" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAB0</accession>
    <name>A0A452HAB0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C19orf12 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HAB0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011660"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013358.1">
        <property type="protein sequence ID" value="ENSGAGP00000011660.1"/>
        <property type="gene ID" value="ENSGAGG00000009009.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033369">
        <property type="entry name" value="C19orf12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31493">
        <property type="entry name" value="NAZO FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31493:SF1">
        <property type="entry name" value="PROTEIN C19ORF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20721">
        <property type="entry name" value="C19orf12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="169" mass="18162" checksum="52F1DF4F60548548" modified="2019-05-08" version="1">MPIDVNDVIHLLCRISEERKMKAAIKHAGQGALVAGATAFLGGLMGGPPGIAIGGAVGGLFGAWMSAGQFKPIPQIILELPPAEQQKLYDDAVAIIRNLDWTDAAQLTALVMGNAALQQKLAAVLINYLSHELRAEIRVFHTEVPHLERSYGKLIMQIGLSQPDEHLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HB76</accession>
    <name>A0A452HB76_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the Crumbs protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000012016"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013760.1">
        <property type="protein sequence ID" value="ENSGAGP00000012016.1"/>
        <property type="gene ID" value="ENSGAGG00000009256.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000081">
        <property type="entry name" value="Crumbs 1, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000208">
        <property type="entry name" value="Crumbs 2, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000612">
        <property type="entry name" value="Crumbs 2, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000130">
        <property type="entry name" value="Crumbs 2, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000039">
        <property type="entry name" value="Crumbs cell polarity complex component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000282">
        <property type="entry name" value="Crumbs cell polarity complex component 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000339">
        <property type="entry name" value="Crumbs cell polarity complex component 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000575">
        <property type="entry name" value="Crumbs, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000061">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000117">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000004">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000080">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000434">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000321">
        <property type="entry name" value="Protein delta homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000784">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF4">
        <property type="entry name" value="UNINFLATABLE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019444551" evidence="16">
        <location>
            <begin position="32"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1419"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="34"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="75"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="115"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="154"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="192"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="230"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="268"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="306"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="345"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="383"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="441"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="479"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="522"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="17">
        <location>
            <begin position="566"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="753"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="17">
        <location>
            <begin position="795"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="973"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="17">
        <location>
            <begin position="1035"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1212"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1250"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1287"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1327"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1366"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="429"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="467"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="552"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="779"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="999"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="1238"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="1254"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="1275"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="1313"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="1354"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="1392"/>
            <end position="1401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1479" mass="161054" checksum="398BDAD110B06A91" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HC20</accession>
    <name>A0A452HC20_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ETS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HC20"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012316"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014104.1">
        <property type="protein sequence ID" value="ENSGAGP00000012316.1"/>
        <property type="gene ID" value="ENSGAGG00000009473.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000066">
        <property type="entry name" value="ETS-related transcription factor Elf-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022084">
        <property type="entry name" value="TF_Elf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF170">
        <property type="entry name" value="ETS-RELATED TRANSCRIPTION FACTOR ELF-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12310">
        <property type="entry name" value="Elf-1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ETS" evidence="11">
        <location>
            <begin position="206"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="137"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="307"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="515"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="311"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="604" mass="63448" checksum="2FD4285117AC52F0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDN9</accession>
    <name>A0A452HDN9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">L-gulonolactone oxidase</fullName>
            <shortName evidence="17">LGO</shortName>
            <ecNumber evidence="5 17">1.1.3.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 17">Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>L-gulono-1,4-lactone + O2 = L-ascorbate + H2O2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32363"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17587"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="EC" id="1.1.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Cofactor biosynthesis; L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway; L-ascorbate from UDP-alpha-D-glucuronate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Microsome membrane</location>
            <topology evidence="17">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Endoplasmic reticulum membrane</location>
            <topology evidence="17">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.8" evidence="5 17"/>
    <dbReference type="AlphaFoldDB" id="A0A452HDN9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012925"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014813.1">
        <property type="protein sequence ID" value="ENSGAGP00000012925.1"/>
        <property type="gene ID" value="ENSGAGG00000009852.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00991">
        <property type="reaction ID" value="UER00939"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003885">
        <property type="term" value="F:D-arabinono-1,4-lactone oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050105">
        <property type="term" value="F:L-gulonolactone oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2520:FF:000001">
        <property type="entry name" value="L-gulonolactone oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007173">
        <property type="entry name" value="ALO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010031">
        <property type="entry name" value="FAD_lactone_oxidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006093">
        <property type="entry name" value="Oxy_OxRdtase_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030654">
        <property type="entry name" value="Sugar_lactone_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01678">
        <property type="entry name" value="FAD_lactone_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43762">
        <property type="entry name" value="L-GULONOLACTONE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43762:SF8">
        <property type="entry name" value="L-GULONOLACTONE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04030">
        <property type="entry name" value="ALO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000136">
        <property type="entry name" value="LGO_GLO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00862">
        <property type="entry name" value="OX2_COVAL_FAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0060">Ascorbate biosynthesis</keyword>
    <keyword evidence="10 17" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 17" id="KW-0274">FAD</keyword>
    <keyword evidence="7 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 17" id="KW-0492">Microsome</keyword>
    <keyword evidence="14 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="18">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU367158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="422" mass="48434" checksum="C05AAD8AA1A45A85" modified="2019-05-08" version="1">SSPELSFQPTSVEEIKEILDLARQRSKRVKVVGGGHSPSDIACTDDFMVQMGKMNKILKVDEEKRQVMVEAGILLSDLNVELSKYGLALPNLGAVSEVAAAGVIGTGTHNTGIKHGILPTQVVALTLLTASGEILECSESINAEIFQAARLHLGCLGVVLTITFQCVPEFYLLETTFPSTLQEVLDNLDSHLRRSEYFRFLWFPHSENVSVIYQDHTNKPPSSSASWFWDYAVGYYLLEFLLWISTFLPGLVCWINRFFFWLLFKGKVEHVDLSYKIFNYECRFRQHVQDWAIPIEKTKEALLELKTVLENNPKVVAHYPVEVRFARGDDILLSPCFQRDSCYMNIIMYRPYGKDVPRLDYWLAYESIMKKAGGRPHWAKAHTCTRKDFEKMYPGFRKFCTIREELDPTGMFLNVYLEKVFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HE68</accession>
    <name>A0A452HE68_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Plectin/eS10 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eS10 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000013095"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014998.1">
        <property type="protein sequence ID" value="ENSGAGP00000013095.1"/>
        <property type="gene ID" value="ENSGAGG00000010043.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001335">
        <property type="entry name" value="40S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005326">
        <property type="entry name" value="Plectin_eS10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037447">
        <property type="entry name" value="Ribosomal_eS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03501">
        <property type="entry name" value="S10_plectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Plectin/eS10 N-terminal" evidence="7">
        <location>
            <begin position="14"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="177" mass="19960" checksum="2CEB61E855D708FD" modified="2019-05-08" version="1">MYQSSSRVSLSQQKNRTRQQTGNLFKEGVMVAKKDVQMAKHPELANSVPNLHVMKAMQSLKSHGYVKEPFAWRHFYWYLTNKGIHYLHDYLHLLPEIVLATLRCSCPETDRLRPKGLEGECPARLTQGEXPPYRLIDTYRRSTAPLDALKKAEAAAGAATKFQFRGGFGGGHGPPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEB1</accession>
    <name>A0A452HEB1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fanconi anemia core complex-associated protein 24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Fanconi anemia-associated protein of 24 kDa</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9, FANCM and FAAP24. Interacts with FANCM.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HEB1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013142"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015047.1">
        <property type="protein sequence ID" value="ENSGAGP00000013142.1"/>
        <property type="gene ID" value="ENSGAGG00000010075.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20076">
        <property type="entry name" value="XPF_nuclease_FAAP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000046">
        <property type="entry name" value="Fanconi anemia core complex-associated protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000006">
        <property type="entry name" value="Fanconi anemia core complex-associated protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026985">
        <property type="entry name" value="FAAP24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040646">
        <property type="entry name" value="PND"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31786">
        <property type="entry name" value="FANCONI ANEMIA CORE COMPLEX-ASSOCIATED PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31786:SF2">
        <property type="entry name" value="FANCONI ANEMIA CORE COMPLEX-ASSOCIATED PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17949">
        <property type="entry name" value="PND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fanconi anemia core complex-associated protein 24 pseudonuclease" evidence="11">
        <location>
            <begin position="14"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="DisA/LigA helix-hairpin-helix motif" evidence="10">
        <location>
            <begin position="168"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="217" mass="23900" checksum="6A2C374E84E2E27D" modified="2019-05-08" version="1">MTAKETTSVEAGAVSVPYGHVIGNEKWRGSQIARGLQGKIKLILEDGLGFVDFHLSNRSCILYISEADLVAGNGFKRRLVRFRNASSLHGIVIVEKTQISDQYFPAVQKFIVLELGMTLLPVANQGEASQLIIQLVHEQSKDHTSNPFLHKKCSQLAEASILRTVQQIPGVGKTKALLLLQQFVSIHQLCNASVQDLELVVGQTIAQQIHAFFTQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEH0</accession>
    <name>A0A452HEH0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HEH0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013201"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015112.1">
        <property type="protein sequence ID" value="ENSGAGP00000013201.1"/>
        <property type="gene ID" value="ENSGAGG00000010114.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF133">
        <property type="entry name" value="RIKEN CDNA 1700020N01 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019095884" description="KRAB domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="2">
        <location>
            <begin position="15"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="73" mass="8412" checksum="D40A96D95A81C3FE" modified="2019-05-08" version="1">PVSAILTLFLGGGLVTFEEVAVYFTAEEWALLDPAQRALYWDVMQENYENVTWLGKSLWTFPSTRDADPRWQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEX5</accession>
    <name>A0A452HEX5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein MON2 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MON2 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000013386"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015319.1">
        <property type="protein sequence ID" value="ENSGAGP00000013386.1"/>
        <property type="gene ID" value="ENSGAGG00000010238.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032817">
        <property type="entry name" value="Mon2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF333">
        <property type="entry name" value="PROTEIN MON2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16206">
        <property type="entry name" value="Mon2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="9">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2/Sec7/BIG1-like HUS" evidence="7">
        <location>
            <begin position="140"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2/Sec7/BIG1-like HDS" evidence="6">
        <location>
            <begin position="779"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2 C-terminal" evidence="8">
        <location>
            <begin position="862"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="441"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1657" mass="183781" checksum="DDB5ED236E65A7E0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGB2</accession>
    <name>A0A452HGB2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGB2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013874"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015882.1">
        <property type="protein sequence ID" value="ENSGAGP00000013874.1"/>
        <property type="gene ID" value="ENSGAGG00000010566.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003288">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001437">
        <property type="entry name" value="Zinc finger protein 594"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001949">
        <property type="entry name" value="zinc finger protein 62 homolog isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001697">
        <property type="entry name" value="zinc finger protein 623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000416">
        <property type="entry name" value="zinc finger protein 879 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050826">
        <property type="entry name" value="Krueppel_C2H2_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24377">
        <property type="entry name" value="IP01015P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="117"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="285"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="438"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="466"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="493"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="521"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="549"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="398"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="593" mass="67270" checksum="CB207D749618B1E9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGY4</accession>
    <name>A0A452HGY4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGY4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014127"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016175.1">
        <property type="protein sequence ID" value="ENSGAGP00000014127.1"/>
        <property type="gene ID" value="ENSGAGG00000010767.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06790">
        <property type="entry name" value="PDZ_neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000010">
        <property type="entry name" value="Neurabin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040645">
        <property type="entry name" value="Neurabin-1/2_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043446">
        <property type="entry name" value="Neurabin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154">
        <property type="entry name" value="NEURABIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154:SF24">
        <property type="entry name" value="NEURABIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17817">
        <property type="entry name" value="PDZ_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="PDZ" evidence="14">
        <location>
            <begin position="494"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="258"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="613"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="669"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="413"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="615"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="815" mass="91049" checksum="30564BBB721A9990" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HH70</accession>
    <name>A0A452HH70_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HH70"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014225"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016281.1">
        <property type="protein sequence ID" value="ENSGAGP00000014225.1"/>
        <property type="gene ID" value="ENSGAGG00000010827.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF163">
        <property type="entry name" value="ZINC FINGER PROTEIN 589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="3"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="73" mass="8148" checksum="5725E09322B6BFE8" modified="2019-05-08" version="1">MPVSFEEVAVYFTQGQGALLDPTQRALYRDVMLENYETVTSLGKGFLSPQILEAVGSACLNLVMFFPCQLDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHI4</accession>
    <name>A0A452HHI4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Low-density lipoprotein receptor-related protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="18">Homooligomer. Interacts with MUSK; the heterodimer forms an AGRIN receptor complex that binds AGRIN resulting in activation of MUSK. Interacts (via the extracellular domain) with SOST; the interaction facilitates the inhibition of Wnt signaling. Interacts with MESD; the interaction promotes glycosylation of LRP4 and its cell-surface expression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000014344"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016412.1">
        <property type="protein sequence ID" value="ENSGAGP00000014344.1"/>
        <property type="gene ID" value="ENSGAGG00000010903.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000029">
        <property type="entry name" value="LDL receptor related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000017">
        <property type="entry name" value="LDL receptor related protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000092">
        <property type="entry name" value="LDL receptor related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000034">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000008">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000085">
        <property type="entry name" value="low-density lipoprotein receptor-related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000098">
        <property type="entry name" value="low-density lipoprotein receptor-related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000006">
        <property type="entry name" value="Putative low-density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF15">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="15 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1774"/>
            <end position="1795"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="24">
        <location>
            <begin position="469"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="531"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="574"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="617"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="661"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="837"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="880"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="923"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="967"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="1145"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="1188"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="1231"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="1275"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="1449"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="1492"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="1535"/>
            <end position="1578"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="21">
        <location>
            <begin position="1579"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1711"/>
            <end position="1742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1911"/>
            <end position="1954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1712"/>
            <end position="1739"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="141"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="261"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="289"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="301"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="321"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="328"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="340"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="363"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00074424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1954" mass="217277" checksum="01B916A465F9D9D2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJ59</accession>
    <name>A0A452HJ59_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJ59"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014942"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017068.1">
        <property type="protein sequence ID" value="ENSGAGP00000014942.1"/>
        <property type="gene ID" value="ENSGAGG00000011292.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03593">
        <property type="entry name" value="CLECT_NK_receptors_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050828">
        <property type="entry name" value="C-type_lectin/matrix_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033992">
        <property type="entry name" value="NKR-like_CTLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710:SF35">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 2 MEMBER D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="80"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="208" mass="23059" checksum="E13DA05D553DD56F" modified="2019-05-08" version="1">MDPTGTKLGLEFYPEIPGNPKSGAEPGNGRSCRLRKHAACTVSLSVVLVVLVVAVVALAVLVLKAQPQFMAWCPDGWIGYRGKCYYFSEAENNWNNSQRHCFELGASLAVIDSDQDLAFMMRYKGISEHWIGLWKEQARQTWKWVNGSDFNSSFPMGGGGDCAYLNDKGVSSSRCITGRNWVCSKPDAYTSQKNATQCQELKSFTMGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKJ9</accession>
    <name>A0A452HKJ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ArfGAP with SH3 domain, ankyrin repeat and PH domain 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HKJ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015462"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017662.1">
        <property type="protein sequence ID" value="ENSGAGP00000015462.1"/>
        <property type="gene ID" value="ENSGAGG00000011672.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043593">
        <property type="entry name" value="ASAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854:SF4">
        <property type="entry name" value="ARF-GAP WITH SH3 DOMAIN, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854">
        <property type="entry name" value="ASAP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="23681" checksum="862F74728F91AB28" modified="2019-05-08" version="1">MPDQISVSEFVSETNEDYKSPTASNFTTRMAQCRNTVAAIEEVRAGGSSGGEGLQSPSGLAPRSARRGGEQGARRCLGKRLSAARRGERRAGPGLSLLPGPPSAGKGWGALGLPLPSYQGRDSAPLRRRGETGWGSFSPRGPVPFLGRGAVAVSVSPTLPGPGAVSPSACHIRPFTSLSPPSSPAPGCVWLHPPVGCYTPAERSCVCLTEGGFLLKKFEIPLAKSFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HLS5</accession>
    <name>A0A452HLS5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Spermatogenesis-defective protein 39 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">VPS33B-interacting protein in apical-basolateral polarity regulator</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">VPS33B-interacting protein in polarity and apical restriction</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Late endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HLS5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015885"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018151.1">
        <property type="protein sequence ID" value="ENSGAGP00000015885.1"/>
        <property type="gene ID" value="ENSGAGG00000011955.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.780">
        <property type="entry name" value="Vps16, C-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040057">
        <property type="entry name" value="Spe-39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038132">
        <property type="entry name" value="Vps16_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13364">
        <property type="entry name" value="DEFECTIVE SPERMATOGENESIS PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13364:SF6">
        <property type="entry name" value="SPERMATOGENESIS-DEFECTIVE PROTEIN 39 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="100"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="520" mass="59624" checksum="D1581E513F2E72CB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNR0</accession>
    <name>A0A452HNR0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HNR0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016605"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018953.1">
        <property type="protein sequence ID" value="ENSGAGP00000016605.1"/>
        <property type="gene ID" value="ENSGAGG00000012415.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050413">
        <property type="entry name" value="TCR_beta_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268:SF31">
        <property type="entry name" value="T CELL RECEPTOR BETA VARIABLE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268">
        <property type="entry name" value="T-CELL RECEPTOR BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="18"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="118" mass="13489" checksum="826E761CC0727016" modified="2019-05-08" version="1">IFLFCHECTPHFISLPPGSSVQLHCYVQRYSSIPWMYWYQQPRQGGALHMLFLYRSKDSIENSLEKHFIGAQPTQQNFTVSTDSLFPNHTGLYYCAWSHTLSQVGAAPGQKLPLPLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRH6</accession>
    <name>A0A452HRH6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein-L-isoaspartate O-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.77</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:12706"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452HRH6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017635"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020119.1">
        <property type="protein sequence ID" value="ENSGAGP00000017635.1"/>
        <property type="gene ID" value="ENSGAGG00000013101.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000027">
        <property type="entry name" value="Protein-L-isoaspartate O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF19">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01279">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018978550" description="Protein-L-isoaspartate O-methyltransferase" evidence="7">
        <location>
            <begin position="24"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="249" mass="27265" checksum="E6083B18C63F1768" modified="2019-05-08" version="1">MSRVLQCAIFTVLMGLLLTRTMAWTSSGKTHAELLNNLYKNGVIKSQRVLDVLLATDRAHYTKYFPYMDSPQSIGYKATISAPHMHAHALELLKDQLVEGAKALDVGSGSGYLTACFARMVGPTGKAVGIEHIEELVHESIRNVREDDPTLLTSGRLKLLVGDGRQGYPEEAPYDAIHVGAAADTVPKELLKELKPGGRLILPVGPAGANQVLMQYDKTSDGQIVETQLMGVIYVPLTDKEKQWPRDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HS09</accession>
    <name>A0A452HS09_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HS09"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017835"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020350.1">
        <property type="protein sequence ID" value="ENSGAGP00000017835.1"/>
        <property type="gene ID" value="ENSGAGG00000013238.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023250">
        <property type="entry name" value="Cyclin-dep_Kinase_2_interact"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15827">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15827:SF2">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02040">
        <property type="entry name" value="CDK2IP"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="239" mass="27243" checksum="C7FA584D38387B63" modified="2019-05-08" version="1">MGGPDVSALPREGGARRGRGTYHGGARGMAAKSPAAVPPKRPVLSVSARKIKDNAADWHNLMMKWEMLNNNGFTTANKIVNLKIDTQFKDNKLEIECDNIASDCGKLSPNYNEELEMFCAKLLETLENMAKIQQKMEKLCSTTKGICDLETYHYENGDCRTPLFHTWPTTYFYEVSFKLTEMYKKEIRLKQTIVQEIAHTADQDLMMVYLSSWLYQPYIESSSKLLLESMLLETGHRQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTV7</accession>
    <name>A0A452HTV7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the receptors of complement activation (RCA) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HTV7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018540"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021140.1">
        <property type="protein sequence ID" value="ENSGAGP00000018540.1"/>
        <property type="gene ID" value="ENSGAGG00000013728.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000055">
        <property type="entry name" value="Complement decay-accelerating factor, GPI-anchored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000014">
        <property type="entry name" value="Membrane cofactor protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050350">
        <property type="entry name" value="Compl-Cell_Adhes-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325">
        <property type="entry name" value="COMPLEMENT COMPONENT-RELATED SUSHI DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325:SF317">
        <property type="entry name" value="COMPLEMENT DECAY-ACCELERATING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="10 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019123597" description="Sushi domain-containing protein" evidence="14">
        <location>
            <begin position="35"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="34"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="99"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="160"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="231"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="294"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="419"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="449"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="497" mass="54312" checksum="EB6C0EEC342ECDD1" modified="2019-05-08" version="1">MWWGLAAMELLRPSWAPRLSALLVLALLPRGALGNCGAPPRLSFAELRDEFKTQTNFTVGTTVRYSCRSGYQKVPGESPTLTCENESVWSEPKEFCRGKSCGHPGDLVNGKVHITDLLFGSSITFSCDEGFRLVGQKTSQCVIDGTGVTWSHELPLCEKIPCLPPPEIDNGKYLGDPNSHYTYGSTVTYSCNTVPRGTDSFSLIGEASIHCTSDEEQNGVWSGEPPECKVVKCSNVEVKNGRKQSGFGPSYTYKDAITFGCDADYFLVGSNVIICQANGSWTPAIPTCEEIPPSVCGTPPFSNGKIFPSKPRYVTGDRITLTCEANYVLSDGAKSMTVHCKENNVWDPPVQTCQLIPSVCSAPAFPNGKILPSKPQYVTGDGITLTCEENYVLLHGEKSMTVHCEGNNVWDPPVQTCLHVCPDLVIPHGTVKSGAKLLYVVGDHVTIECSTFHAMHGEPNIQCTDEFQWKPAIPECKLRKYKCNILKNGPKNIFHIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HU40</accession>
    <name>A0A452HU40_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HU40"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018562"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021162.1">
        <property type="protein sequence ID" value="ENSGAGP00000018562.1"/>
        <property type="gene ID" value="ENSGAGG00000013737.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016037">
        <property type="term" value="P:light absorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15082">
        <property type="entry name" value="7tmA_VA_opsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000238">
        <property type="entry name" value="Opsin-VA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002206">
        <property type="entry name" value="Opsin_pineal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00666">
        <property type="entry name" value="PINOPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0157">Chromophore</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 13" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="3 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="103"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="55"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="387" mass="44038" checksum="737AA5B42D35F6D4" modified="2019-05-08" version="1">MDVFRAPENESLLSGPTASSTPWDPFHHPLEFIQPWNFQLLAALMFLVTSLSLSENFAVILVTFKFKQLRQPVNYVIVNLAVADFLVSLIGGTISFFTNIKGYFYMGHWACVLEGFAITFFGIVGLWSLALLAFERYIVICRPLGNMRLRGKHAALGIIFVWIFSFIWTIPPTMGWSSYTTSKIGTTCEPNWYSGTYNDHTYIITFFTTCFILPLLVILVSYGKLMRKLRKVSDTQGRLGTTRKPERQVTRMVIIMILAFLICWSPYAAFSILVTAWPSIYLDPRLAAIPAFFSKTATVYNPIIYVFMNKQFRRCFIQLFGCSERETAVSNMNPTLERVVLTQDKGGGEMFTIAAHNTISNRRNEDEQRSCHSLAQLTVSENKVCPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVC3</accession>
    <name>A0A452HVC3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase</fullName>
            <ecNumber evidence="9">3.6.1.62</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = N(7)-methyl-GDP + a 5'-end phospho-ribonucleoside in mRNA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67484"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:63714"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="EC" id="3.6.1.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67485"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DCP1 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.62" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452HVC3"/>
    <dbReference type="SMR" id="A0A452HVC3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019076"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021743.1">
        <property type="protein sequence ID" value="ENSGAGP00000019076.1"/>
        <property type="gene ID" value="ENSGAGG00000014114.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09804">
        <property type="entry name" value="Dcp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000097">
        <property type="entry name" value="Putative mRNA-decapping enzyme 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010334">
        <property type="entry name" value="Dcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031953">
        <property type="entry name" value="mRNA_decap_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290:SF5">
        <property type="entry name" value="MRNA-DECAPPING ENZYME 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290">
        <property type="entry name" value="TRANSCRIPTION FACTOR SMIF DECAPPING ENZYME DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06058">
        <property type="entry name" value="DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16741">
        <property type="entry name" value="mRNA_decap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="mRNA-decapping enzyme C-terminal" evidence="12">
        <location>
            <begin position="567"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="408"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="525"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="609" mass="66917" checksum="9724275C3C09CFB5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWM5</accession>
    <name>A0A452HWM5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Septin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HWM5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019518"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022221.1">
        <property type="protein sequence ID" value="ENSGAGP00000019518.1"/>
        <property type="gene ID" value="ENSGAGG00000014385.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000064">
        <property type="entry name" value="Septin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="11">
        <location>
            <begin position="119"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="site" description="Important for dimerization" evidence="8">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR006698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR006698-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="447" mass="51024" checksum="DA473AC558868500" modified="2019-05-08" version="1">MVGCWRRKGRARGADWLSAVGWPGLHERSPGPAQLSNAAAGAAPADTRTRTALSAAGRPASPCLCRAGALGSRRPERRMSTGLRYKSKLVNPEEKQDHEKQYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLVNSLFLTDLYKDRKLLNAEERISQTVEILKHTVDIEEKGVKLKLTIVDTPGFGDAVNNTECWKPITDYIDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPVDVQFMKALHEKVNIVPLIAKADCLIPSEIRKLKERIREEIDKFGIKVYQFPDCDSDEDEEFKQQDRELKESAPFAVIGSNTVVEAKGQRVRGRLYPWGIVEVENQAHCDFVKLRNMLIRTHMHDLKDVTCDVHYENYRAQCIQQMTSKLTQDNRIESPIPILPLPTPDAETEKLIKMKDEELRRMQEMLQKMQQQMQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWT9</accession>
    <name>A0A452HWT9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HWT9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019660"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022395.1">
        <property type="protein sequence ID" value="ENSGAGP00000019660.1"/>
        <property type="gene ID" value="ENSGAGG00000014505.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15911">
        <property type="entry name" value="7tmA_OR11A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="139"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="40"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="323" mass="36070" checksum="3D0968825FE1FB8A" modified="2019-05-08" version="1">MFDNRTIITEFILLGFGNLPELQILLFLVFLVIYIVTMSGNILITALVVADQHLHTPMYFFLGNLSCLEMCYTSTVLPRLLASLLTGDRTISVSGCFAQFYCFGSLAATECYLLAAMSYDRYLAICKPLRYAALMTGRLCLQLAAGSWISGFLICIIIMCFMSQLTFCGPSEIDHFFCDFSPMLKLSCSDTSMITLVSFILASLDSPCPFLLTMTSYVCIIATILRIPSTTGRKKAFSTCSSHLIVVTVFYGTLMTVYLLPKTNTLKALNKVFSVFYTVLTPMLNPLIYSLRNKEVKEALRKTLVCGWKIQKHKAVSVQHPLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXE4</accession>
    <name>A0A452HXE4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Grh/CP2 DB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the grh/CP2 family. CP2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HXE4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022622.1">
        <property type="protein sequence ID" value="ENSGAGP00000019855.1"/>
        <property type="gene ID" value="ENSGAGG00000014627.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000079">
        <property type="entry name" value="transcription factor CP2-like protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007604">
        <property type="entry name" value="CP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057520">
        <property type="entry name" value="GRHL1/CP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041418">
        <property type="entry name" value="SAM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040167">
        <property type="entry name" value="TF_CP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037:SF12">
        <property type="entry name" value="GRH_CP2 DB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037">
        <property type="entry name" value="TRANSCRIPTION FACTOR CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04516">
        <property type="entry name" value="CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25416">
        <property type="entry name" value="GRHL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18016">
        <property type="entry name" value="SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51968">
        <property type="entry name" value="GRH_CP2_DB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Grh/CP2 DB" evidence="9">
        <location>
            <begin position="49"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="229"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="481" mass="54262" checksum="00DC4134C4CDDEA4" modified="2019-05-08" version="1">MLFWHSQPEHYWPGPGEMYPCSGGNLLRESLALPFLKQEEQNNISTSAELHCPAFQYVLCTPTSPAIKQHEETLTYLNQGQSYEIRMLCNPKLGDFSECRKLLKSVARVVFHDRRLQYTEHQQLEGWRWNRPGDRILDIDIPMSVGVLEPHIHPTLLNTVEFLWDPTKRTSIFMQVHCISTEFTLRKNGGEKGVPFRIQIDTYKANEKGEHVDHLHSASCLVKVFKPKGADRKQKTDREKIEKQSPQEREKYQPSYESTVLAECGPWPEASSSPQSPPATPGLPSPHAFKLLPPERGCSSPSCPSDSPAENTAEALNPCASILETQQWLHKNRFSSYCRMLANFTGADLLKLSRSDLIQICGAADGIRLSNALKARCVRPRLTLYMSREPQANGRHTPEDSPPATYQEVYLEELTATELTSKLAELLSLPANQIQQVSKQGPMGIHILISDLMVRNLPDESCFIAAVTKVQAPEGYHLILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZJ4</accession>
    <name>A0A452HZJ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tripartite motif containing 39</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HZJ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020664"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023539.1">
        <property type="protein sequence ID" value="ENSGAGP00000020664.1"/>
        <property type="gene ID" value="ENSGAGG00000015220.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19762">
        <property type="entry name" value="Bbox2_TRIM7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12888">
        <property type="entry name" value="SPRY_PRY_TRIM7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="13"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="90"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="7">
        <location>
            <begin position="248"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="450" mass="51866" checksum="5375E41BA30C85D8" modified="2019-05-08" version="1">MVSGFLVQDEAVCPICLEYLTEPVTLDCGHNFCLACITKHSETCTEGDYDPLCCPSCRAQIRRQNFQLNWQLANRVEQVKHLHVEAEREQKVNLCARHKEELKLFCEDDREAICVICRESKEHRAHTVVPTEDSAQEDKVGNKQGNRMLGIMKKEKDKTENIILPLYKSMVHPHCEYCMQMWLPHLKKVILELEKVQKRARKIIRVMEQGNMTEVYNIMTGVEKVNKEVLFTPSHSTRTRSHQMKLIGRRFKANKRKYFFTQRTVMNVTLDPDTAHPRLVLSEDQKSVRWGFTRQNLPNNLERFDNDPFVLGCEGFTSGRHCWEVEVGEARSWVLGVARESVSRKGRISVSPEQGFWALRRSGAGWLVAYTSPEGTHLPFADKPRRIRVSLDYEQGQVTFFDVDNEALIFTFPPASFSGDKIRPFFSVGERGIYTSFSYLCGPSHITVCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I074</accession>
    <name>A0A452I074_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I074"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020854"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023749.1">
        <property type="protein sequence ID" value="ENSGAGP00000020854.1"/>
        <property type="gene ID" value="ENSGAGG00000015341.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000349">
        <property type="entry name" value="Heat shock transcription factor, Y-linked"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF336">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR, Y-LINKED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="8">
        <location>
            <begin position="83"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="394" mass="45378" checksum="FBDE65670CA36F18" modified="2019-05-08" version="1">MFDTAVALISTDKKTFSPEKSEKKLSSAVSLMLDQNMPGDLSVKDILEEHAYEVQEEASFSKKLHCHSCNQSKVETGEINVLLSLSFPKKLWKIVESDEFKSIGWNDHGDCVIIDEICFQREILDRRGILRIFDTDSMKSFIRQLNLYGFSKIRKNVTLPRFQAEKNRTRARILLYHNPYFRRECPFLIQRMKRRVGIKSNEQPNPISPNLKRRKPAAPKKYISFQDSGQANDLQTPCLQETMHSNLQNNSINAWNGKMNHSYPVSTPWPLENGTENSIQNGIYNQQRNPSMDTNATSSICPYFPLTSETGLEPSMELHRLPSTYQDFVFMNAQFTTLMSFWNPWFSMLMAATSPIPLTETLNQQFTFPGQTCSLCNCYGNTERSKLHHPTNEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I112</accession>
    <name>A0A452I112_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I112"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021174"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024115.1">
        <property type="protein sequence ID" value="ENSGAGP00000021174.1"/>
        <property type="gene ID" value="ENSGAGG00000015559.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF142">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="7"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="96" mass="10916" checksum="7EE34A244AE2C4F2" modified="2019-05-08" version="1">MNDTMPVTFEEVAVYFTQGQGALLDPAQRALYRDVMQENYEMVTSLGKGFSSPQLLESVGPVLLSLVRSHVLSHLYCFSFTQRIPFHLPPWELLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1S7</accession>
    <name>A0A452I1S7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 3 subunit A</fullName>
            <shortName evidence="8">eIF3a</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Eukaryotic translation initiation factor 3 subunit 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">eIF-3-theta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">EIF3A</name>
        <name evidence="8" type="synonym">EIF3S10</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="function">
        <text evidence="7">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the eIF-3 subunit A family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000021426"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024402.1">
        <property type="protein sequence ID" value="ENSGAGP00000021426.1"/>
        <property type="gene ID" value="ENSGAGG00000015734.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.860:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.860:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.860">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03000">
        <property type="entry name" value="eIF3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027512">
        <property type="entry name" value="EIF3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054711">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3, THETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22591">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="initiator methionine" description="Removed" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="PCI" evidence="10">
        <location>
            <begin position="205"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="719"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="744"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="448"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="557"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="744"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1089"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1235"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1252"/>
            <end position="1282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1292" mass="154382" checksum="695275E0F3741525" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1T7</accession>
    <name>A0A452I1T7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GDP/GTP exchange factor Sec2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the SEC2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I1T7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021438"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024420.1">
        <property type="protein sequence ID" value="ENSGAGP00000021438.1"/>
        <property type="gene ID" value="ENSGAGG00000015744.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070319">
        <property type="term" value="C:Golgi to plasma membrane transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4880:FF:000001">
        <property type="entry name" value="Guanine nucleotide exchange factor for Rab-3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040351">
        <property type="entry name" value="RAB3IL/RAB3IP/Sec2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009449">
        <property type="entry name" value="Sec2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430:SF5">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR FOR RAB-3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430">
        <property type="entry name" value="RABIN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25555">
        <property type="entry name" value="RAB3A-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06428">
        <property type="entry name" value="Sec2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144284">
        <property type="entry name" value="Sec2 N-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GDP/GTP exchange factor Sec2 N-terminal" evidence="9">
        <location>
            <begin position="60"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="80"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="435" mass="48712" checksum="272C26B8AD45D920" modified="2019-05-08" version="1">MFHTLVSPSQAPFKEDSQQLQKQPVVGQWKPLASSSSGKELPQLDPHCREAGGGDDDQSTAQRNVSRLRSSSVEIREKGSEFLKEELHRAQKELKLKDEECERLSKVREQLEQELEELTASLFEEAHKMVRDANTKQAASEKQLREARGKIDMLQAEVTALKTLVITSTPSSPNRELHPQLQSPSKAVFRKGHGRNKSASSTVVTATSQSLPLEPVSNECKEFGVPTLLSLLLCIVPGKAIHITYEPGWQALVADPSSSSSLRQVDSILFAEFQAWKESPTLEKSCSFLERIFREDVGPCLDFTKQELSELVREAVEQNTLTIEPVAIQALPVVKVSAMECGGPNGFRSQIVTKCALSGLSRACKHRIKLGDSGNYYYISPSCRARITAVCNFFTYIRYIQQGLVRQDVELMYWEIMRLRREMSMAKLGFYPSAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I227</accession>
    <name>A0A452I227_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I227"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024544.1">
        <property type="protein sequence ID" value="ENSGAGP00000021551.1"/>
        <property type="gene ID" value="ENSGAGG00000015814.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026618">
        <property type="entry name" value="MPLKIP-like_vertebrate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028265">
        <property type="entry name" value="TTDN1/SICKLE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22446">
        <property type="entry name" value="M-PHASE-SPECIFIC PLK1-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22446:SF3">
        <property type="entry name" value="M-PHASE-SPECIFIC PLK1-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15502">
        <property type="entry name" value="MPLKIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="190" mass="20593" checksum="49E733582074C9CE" modified="2019-05-08" version="1">MHRQSFRPPTPPYPGGGPWGGGFRSPPSGGGPVPPSPRGYGSPHHTPPYGHRPYSPRGHSFHGGGGRFGSPSPGGQSPRRPHSASPRYSAPYGNVSPAAAQQRPPHHPQQYKSSPRGSQRYYQGSPRTSTPFGTAHGRGKRVSNDVESYYRPSMLEDPWAGLEPVSVTDINQQYSSEQTTYTGRRFHPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3D8</accession>
    <name>A0A452I3D8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I3D8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022038"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025107.1">
        <property type="protein sequence ID" value="ENSGAGP00000022038.1"/>
        <property type="gene ID" value="ENSGAGG00000016169.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF163">
        <property type="entry name" value="ZINC FINGER PROTEIN 589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="2">
        <location>
            <begin position="14"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="153" mass="17068" checksum="285873C909294625" modified="2019-05-08" version="1">MINPVGFLLFQGTVTFKEVAVYFTREEGALLDSAQRALYRDVMQENYENVTLLCKGSCPLISSFPLPRTKVHASPPVPPLLCPGSASPQYASDTHTSTEPGTRREGWGESEIYTFPQILEHGYNMFANVVHFCSIHLSNIDVKYTQIVPLTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4U2</accession>
    <name>A0A452I4U2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GIPC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I4U2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022536"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025673.1">
        <property type="protein sequence ID" value="ENSGAGP00000022536.1"/>
        <property type="gene ID" value="ENSGAGG00000016526.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23077">
        <property type="entry name" value="PDZ_GIPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000097">
        <property type="entry name" value="PDZ domain-containing protein GIPC1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056814">
        <property type="entry name" value="GIPC1-3_GH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017379">
        <property type="entry name" value="GIPC1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12259:SF4">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN GIPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12259">
        <property type="entry name" value="RGS-GAIP INTERACTING PROTEIN GIPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25083">
        <property type="entry name" value="GIPC1_GH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="131"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="328" mass="35677" checksum="1E6BDE628535402E" modified="2019-05-08" version="1">MPLGLGRRKKAPPLVENEEAEPIRGSLGGVEVGSGGSVAALQPGLPPPPASLRPRLVFHTQLAHGSPTGRIEGFTNVKELYSKIAEAFKIPPVEVMFCTLNTHKVDMDKLLGGQIGLEDFIFAHTKGQKKEVEVFKSEEALGLTITDNGAGYAFIKRIKEGSVIDRIQVINVGDMIESINGQSLIGCRHYEVAKMLKDLPKGRTFTLKLTEPRKAFDMISQRAAGGKHNSSSQLGTGRGTLRLRAKGPATVEEQVGASPGELPLTALGSLRIFTWALTFSQRELRVWSQSRMRGKLRSQLSQEWGGDRSSIWGRSLHCVCFPVSSPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4X2</accession>
    <name>A0A452I4X2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Next to BRCA1 gene 1 protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Neighbor of BRCA1 gene 1 protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Myofibril</location>
            <location evidence="11">Sarcomere</location>
            <location evidence="11">M line</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I4X2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022617"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025764.1">
        <property type="protein sequence ID" value="ENSGAGP00000022617.1"/>
        <property type="gene ID" value="ENSGAGG00000016580.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070013">
        <property type="term" value="C:intracellular organelle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14947">
        <property type="entry name" value="NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06396">
        <property type="entry name" value="PB1_NBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14319">
        <property type="entry name" value="UBA_NBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02340">
        <property type="entry name" value="ZZ_NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000033">
        <property type="entry name" value="Next to BRCA1 gene 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000291">
        <property type="entry name" value="Next to BRCA1 gene 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000007">
        <property type="entry name" value="Next to BRCA1 gene 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000199">
        <property type="entry name" value="next to BRCA1 gene 1 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032350">
        <property type="entry name" value="Nbr1_FW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034852">
        <property type="entry name" value="PB1_Nbr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056893">
        <property type="entry name" value="UBA_Nbr1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930:SF2">
        <property type="entry name" value="NEXT TO BRCA1 GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930">
        <property type="entry name" value="OVARIAN CARCINOMA ANTIGEN CA125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16158">
        <property type="entry name" value="N_BRCA1_IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24932">
        <property type="entry name" value="UBA_NBR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PB1" evidence="19">
        <location>
            <begin position="4"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="18">
        <location>
            <begin position="220"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="17">
        <location>
            <begin position="944"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="501"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="694"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="295"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="702"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="719"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="741"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="998" mass="111004" checksum="BEA7200F98FDEFBB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6M9</accession>
    <name>A0A452I6M9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I6M9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023227"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026462.1">
        <property type="protein sequence ID" value="ENSGAGP00000023227.1"/>
        <property type="gene ID" value="ENSGAGG00000017029.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08321">
        <property type="entry name" value="Pyrin_ASC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004020">
        <property type="entry name" value="DAPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02758">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01289">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50824">
        <property type="entry name" value="DAPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="103" mass="11856" checksum="C6CB09EAE031BD63" modified="2019-05-08" version="1">MGKTVRDHLVDTLEELGQDQFKKFKTKLNAFPVREGYGNIPRGRLEKADALDVCDKLISFYREEYAVEVTVEVLTDINERELAVLDFTKTGHHSETNILQQMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7Q9</accession>
    <name>A0A452I7Q9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein sprouty homolog 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sprouty family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I7Q9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023567"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026855.1">
        <property type="protein sequence ID" value="ENSGAGP00000023567.1"/>
        <property type="gene ID" value="ENSGAGG00000017278.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040037">
        <property type="term" value="P:negative regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046580">
        <property type="term" value="P:negative regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007875">
        <property type="entry name" value="Sprouty"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051192">
        <property type="entry name" value="Sprouty_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12365:SF8">
        <property type="entry name" value="PROTEIN SPROUTY HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12365">
        <property type="entry name" value="SPROUTY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05210">
        <property type="entry name" value="Sprouty"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51227">
        <property type="entry name" value="SPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="54"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="313" mass="34502" checksum="76E4D93AA5113163" modified="2019-05-08" version="1">METRAQNGSGSQSLLQVRHDGGRQQGDSDLRDVLTQQVHVLSLDQIRAIRNTNEYTEGPTVAPRPGVKPAPRPASQHKNERPHGLPEHRHLTRVQHAQVHTSSRAPLSRSISTVSTGSRSSTRTSTSSNSSEHRLLGSSSGPMADGIIRVQPKSEFKTSDLKPLSKEDLGMHAYRCEDCGKCKCKECTSPRTLPSCWICDKQCLCSAQNVVDYGTCVCCVKCLFYHCSNDDEDNCADNPCSCSQSHCCTRWSAMGVVSLFLPCLWCYLPAQGCLKLCQGCYDRVNRPGCRCKHSNTVCCKVPSIPPRTFEKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I821</accession>
    <name>A0A452I821_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the POU transcription factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I821"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023789"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027095.1">
        <property type="protein sequence ID" value="ENSGAGP00000023789.1"/>
        <property type="gene ID" value="ENSGAGG00000017409.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000056">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000007">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF41">
        <property type="entry name" value="POU DOMAIN, CLASS 4, TRANSCRIPTION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="12">
        <location>
            <begin position="231"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="324"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="326"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="37"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="133"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="390" mass="42040" checksum="D8623348883CE411" modified="2019-05-08" version="1">MMMMSLNSKQPFSMPHGSGSLHEPKYSALHTASPCTSAAAAPSASSPSSTSSGAGRSSTSTSSSSEAMRRACLPTPPSNIFGGLDESLLARAEALAAVDIVSQTKSHHHHPPHHSPFKPDATYHTMNTIPCTSAASSSSVPISHPSALSGTHHHHHHHHHHHHQPHQALEGELLDHITPGLALGAMTGPDGSVVSTPAHAPHMASMNPMHQAALSMAHAHGLPSHMGCMSDVDADPRDLEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLTKPELFNGAEKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9G8</accession>
    <name>A0A452I9G8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I9G8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024315"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027679.1">
        <property type="protein sequence ID" value="ENSGAGP00000024315.1"/>
        <property type="gene ID" value="ENSGAGG00000017771.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05325">
        <property type="entry name" value="carb_red_sniffer_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051468">
        <property type="entry name" value="Fungal_SecMetab_SDRs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544:SF38">
        <property type="entry name" value="C-FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="27392" checksum="7AFF87E503E372A7" modified="2019-05-08" version="1">MAGFNVRTVLVTGANRGIGLELVKQFLEKPNPPKWVFATCRDPEGERALTPAPHPHIIPKPASSTDTASIKAAATRVEEHLKGSGLNLLINNAAIGGNGTLESETLDDMSLVYTTNVTGPLLVSQAFLPLLKKAAQGSNQKGLSCSKAAIVNMSSEAGSIQNVFIWHLGQAIAYRCSKAALNMLTKCQSLGYEGDGILSIAVHPGWVQTDMGSFMVGTLLGEAPVTVDASVRGILNVLAKLSEKDNGAFVSWEGKVLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9S9</accession>
    <name>A0A452I9S9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Zinc finger protein 212</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I9S9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024443"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027818.1">
        <property type="protein sequence ID" value="ENSGAGP00000024443.1"/>
        <property type="gene ID" value="ENSGAGG00000017857.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000383">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000496">
        <property type="entry name" value="Zinc finger with KRAB and SCAN domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF70">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="15">
        <location>
            <begin position="141"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="291"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="433"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="461"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="490"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="517" mass="59370" checksum="7F3ACEB0CCBA748E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAH2</accession>
    <name>A0A452IAH2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bromodomain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Homodimer. Interacts with E2F1. Interacts with (acetylated) STAT3; promoting STAT3 recruitment to chromatin. Interacts with CTCF; promoting BRD2 recruitment to chromatin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the BET family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IAH2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024699"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028103.1">
        <property type="protein sequence ID" value="ENSGAGP00000024699.1"/>
        <property type="gene ID" value="ENSGAGG00000018031.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05497">
        <property type="entry name" value="Bromo_Brdt_I_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05498">
        <property type="entry name" value="Bromo_Brdt_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000003">
        <property type="entry name" value="Bromodomain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.220:FF:000001">
        <property type="entry name" value="bromodomain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000002">
        <property type="entry name" value="Bromodomain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043508">
        <property type="entry name" value="Bromo_Brdt_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043509">
        <property type="entry name" value="Bromo_Brdt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050935">
        <property type="entry name" value="Bromo_chromatin_reader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880:SF240">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880">
        <property type="entry name" value="FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 15" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="17">
        <location>
            <begin position="90"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="17">
        <location>
            <begin position="363"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="18">
        <location>
            <begin position="618"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="272"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="452"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="694"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="316"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="478"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="542"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="592"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="700"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="761"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="787" mass="86775" checksum="49E5A6E48329D85C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAI0</accession>
    <name>A0A452IAI0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Vascular endothelial growth factor D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">c-Fos-induced growth factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Homodimer; non-covalent and antiparallel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the PDGF/VEGF growth factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IAI0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024673"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028075.1">
        <property type="protein sequence ID" value="ENSGAGP00000024673.1"/>
        <property type="gene ID" value="ENSGAGG00000018011.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042056">
        <property type="term" value="F:chemoattractant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005172">
        <property type="term" value="F:vascular endothelial growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050930">
        <property type="term" value="P:induction of positive chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045766">
        <property type="term" value="P:positive regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060754">
        <property type="term" value="P:positive regulation of mast cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048010">
        <property type="term" value="P:vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038084">
        <property type="term" value="P:vascular endothelial growth factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00135">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000021">
        <property type="entry name" value="vascular endothelial growth factor D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004153">
        <property type="entry name" value="CXCXC_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023581">
        <property type="entry name" value="PD_growth_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000072">
        <property type="entry name" value="PDGF/VEGF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050507">
        <property type="entry name" value="PDGF/VEGF_growth_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12025">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12025:SF11">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03128">
        <property type="entry name" value="CXCXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00341">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00141">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00249">
        <property type="entry name" value="PDGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50278">
        <property type="entry name" value="PDGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0339">Growth factor</keyword>
    <keyword evidence="13" id="KW-0497">Mitogen</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Platelet-derived growth factor (PDGF) family profile" evidence="18">
        <location>
            <begin position="80"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="336" mass="38320" checksum="B4D396F80E38F444" modified="2019-05-08" version="1">MFQGSDYEYGPVKRASLSALERSEQLIRNAASLEELLRITHAEDWKLWKCRLKLKSLSHLDSRSASHRSTRFAAAFYDIETLRVIDEEWQKTQCMPRETCVEVAKELGTTTNKFFKPPCVNVFRCGGCCNEESLGCMNTSTTYVSKTLFEISVPLTSVPEPVPVKIANHTGCRCLSTAQHHQYAIIRRSVQYPEEDGCPFTNKLCHSGLIWDSNKCECVVDKEHPGRREGLPPLAELAKCGPHMEFDEDNCECICRWKCPTDFFQNKENCSCYLCRESQESCSQKHKIFHAETCSCEDKCPFQPRTCSTAKPVCTKHCRCPKEKRSFHGSQSKETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICV8</accession>
    <name>A0A452ICV8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>dopamine(in) + 2 H(+)(out) = dopamine(out) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73739"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:73740"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>serotonin(in) + 2 H(+)(out) = serotonin(out) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73743"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:73744"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Vesicular transporter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ICV8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025601"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029129.1">
        <property type="protein sequence ID" value="ENSGAGP00000025601.1"/>
        <property type="gene ID" value="ENSGAGG00000018700.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000029138.1">
        <property type="protein sequence ID" value="ENSGAGP00000025605.1"/>
        <property type="gene ID" value="ENSGAGG00000018700.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000029139.1">
        <property type="protein sequence ID" value="ENSGAGP00000025606.1"/>
        <property type="gene ID" value="ENSGAGG00000018700.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005335">
        <property type="term" value="F:serotonin:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015842">
        <property type="term" value="P:aminergic neurotransmitter loading into synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17384">
        <property type="entry name" value="MFS_SLC18A1_2_VAT1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000083">
        <property type="entry name" value="synaptic vesicular amine transporter isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000116">
        <property type="entry name" value="synaptic vesicular amine transporter isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00880">
        <property type="entry name" value="2_A_01_02"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF30">
        <property type="entry name" value="SYNAPTIC VESICULAR AMINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="14">
        <location>
            <begin position="69"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="519" mass="56636" checksum="3088CC4FB7AF3B28" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ID84</accession>
    <name>A0A452ID84_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ID84"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025728"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029269.1">
        <property type="protein sequence ID" value="ENSGAGP00000025728.1"/>
        <property type="gene ID" value="ENSGAGG00000018778.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="357"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="443" mass="48438" checksum="88EF0D765CB1EA94" modified="2019-05-08" version="1">MRAQVSAIHQSFVDPKFINSKAQVTIYPFMSQAVDLPTAELPVQYKGWSGMWTFAVYDNYPIPMLLGEDLANQVRRAKRVGMVTRSQTRQASRPIPVPEPSTEAPSVLPETQTEVVDPDPLPTTETATVTPVPDLELEKQPASAPAIANQSSTPKPEGASEPELAKAADNHTQEAQPQPEIPLSAPAESGSPATKTTPSPTSLPEGPSPSPQSEEELVTPASREQFQAEQEADDSLQKAWAAARSTPPPLSSSNRSRFVIDQGLLYKEILSGGHREEWQPQKQLVVPTKYRGKLLSLAHDHPSGHAGVNRTKDRLGKSFHWEGMGKDVAKYVRSCEVCQRVGKPQDQVKAPLQPLPIIEVPFQRVAVDILGPFPKKTPRGKQYVLTLVDFATRWPEAVALGNTRANTVCLALTDIFARVGWPSDILTDSGSNFLAGTMEKLWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IEL0</accession>
    <name>A0A452IEL0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IEL0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026354"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029965.1">
        <property type="protein sequence ID" value="ENSGAGP00000026354.1"/>
        <property type="gene ID" value="ENSGAGG00000019215.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106217">
        <property type="term" value="P:tRNA C3-cytosine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037380">
        <property type="entry name" value="DALRD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16043:SF1">
        <property type="entry name" value="DALR ANTICODON-BINDING DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16043">
        <property type="entry name" value="DALRD3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="493"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="652" mass="70332" checksum="BF23B813EFE0808B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFD8</accession>
    <name>A0A452IFD8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Complex III subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Complex III subunit IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Cytochrome b-c1 complex subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Cytochrome c1, heme protein, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </cofactor>
        <text evidence="21">Binds 1 heme c group covalently per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)). Interacts with FLVCR2; this interaction occurs in the absence of heme and is disrupted upon heme binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IFD8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026600"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030237.1">
        <property type="protein sequence ID" value="ENSGAGP00000026600.1"/>
        <property type="gene ID" value="ENSGAGG00000019377.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000002">
        <property type="entry name" value="Cytochrome c1, heme protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000003">
        <property type="entry name" value="Cytochrome c1, heme protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002326">
        <property type="entry name" value="Cyt_c1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021157">
        <property type="entry name" value="Cyt_c1_TM_anchor_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266">
        <property type="entry name" value="CYTOCHROME C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266:SF3">
        <property type="entry name" value="CYTOCHROME C1, HEME PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02167">
        <property type="entry name" value="Cytochrom_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00603">
        <property type="entry name" value="CYTOCHROMEC1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81496">
        <property type="entry name" value="Cytochrome c1 subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase), transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 21 22" id="KW-0349">Heme</keyword>
    <keyword evidence="12 21 22" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 21 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="24">
        <location>
            <begin position="138"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="21">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="21">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="21">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="21">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR602326-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="355" mass="38649" checksum="26B648DDAB4E4A17" modified="2019-05-08" version="1">MLCLEACCIPCTRRLPAPPPSSPGAGEGSTGSNWVPLGSTSHRAGVDSGECGWGRRGGCPCWSWQAERLPSVFQASMSSLSGMSRGRKVALSALGVLAAGGAGLALALHTAVSASDLQLHPPSYAWSHNGLLASLDHSSIRRGYQVYKQVCAACHSMDYLAFRNLIGVSHTEAEAKALAEEAEVVDGPDENGEMFTRPGKLSDYFPKPYPNHEAARAANNGALPPDLSYIISARHGGEDYVFSLLTGYCDPPTGVVIREELHYNPYFPGQAITMAPPIYDEILEFEDGTPATMSQIAKDVCTFLRWAAEPEHDHRKRMGMKMLMMIGLLTPLIYYLKRHKWSVLKSRKIAYRPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFS6</accession>
    <name>A0A452IFS6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000026806"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030468.1">
        <property type="protein sequence ID" value="ENSGAGP00000026806.1"/>
        <property type="gene ID" value="ENSGAGG00000019521.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000646">
        <property type="entry name" value="Cyclin dependent kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF508">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="16"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="115" mass="13614" checksum="1CB36BDB7FE2465B" modified="2019-05-08" version="1">MALCFQLGRCQSMKEFEKLNRIGEETYGIMYRARDTQTDKIVALKKVQMDKEKDGIPISSLWEITLLLKLWHPNIMELKEVVMGNHLESIFLVKGYCEQDLXYRWIVPVHYHSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGQ8</accession>
    <name>A0A452IGQ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA ligase 1</fullName>
            <ecNumber evidence="3">6.5.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">RNA ligase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Functions as an RNA ligase, in vitro. The ligation reaction entails three nucleotidyl transfer steps. In the first step, the RNA ligase reacts with ATP in the absence of nucleic acid to form a covalent ligase-AMP intermediate and release pyrophosphate. In step 2, the ligase-AMP binds to the nucleic acid and transfers the adenylate to the 5'-PO4 terminus to form an adenylylated intermediate. In step 3, the RNA ligase directs the attack of the 3'-OH on the 5'-phosphoanhydride linkage, resulting in a repaired 3'-5' phosphodiester and release of AMP. Exhibits selectivity for single-stranded RNA substrates and may not have nick-sealing activity on double-stranded DNA-RNA hybrids. May play a role in maintaining RNA integrity under stress conditions, for example in response to reactive oxygen species (ROS).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.5.1.3" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452IGQ8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030793.1">
        <property type="protein sequence ID" value="ENSGAGP00000027101.1"/>
        <property type="gene ID" value="ENSGAGG00000019712.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041211">
        <property type="entry name" value="RLIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31219">
        <property type="entry name" value="CHROMOSOME 28 C12ORF29 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31219:SF2">
        <property type="entry name" value="RNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17720">
        <property type="entry name" value="RLIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0692">RNA repair</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="325" mass="37424" checksum="458F380355A2217D" modified="2019-05-08" version="1">MSRLGSVQRKVPCLFVTQVKEEPSAKRERQPFKVLATETINRKALDADIYNAVPTEKVDGTCCYVTTYKGQPYLWARLDRKPNKLSEKRFKRFMYSVEDSKEFIWNIEEDFRPVPECWIPAKEIQQSSGNPLPDENGHIPGWVPVEKNSKQYCWHSSVVNYEAEIALILKCHADDPGLLEISSVPLSDLLEQTLELIGTNINANPYGLGSKKHPVHLLVPHGAFEIKNPPMLKHSDILSWFESCREGKIEGIVWHCNDGHLIKLHRHHLGMCWPIPETYLNSQPVVIAVNRTKYDCDFEPKCLFNHFSKLDGQRFSRLKDIKFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHB6</accession>
    <name>A0A452IHB6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IHB6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027383"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031110.1">
        <property type="protein sequence ID" value="ENSGAGP00000027383.1"/>
        <property type="gene ID" value="ENSGAGG00000019915.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="314" mass="35093" checksum="1A3558D844FCFB10" modified="2019-05-08" version="1">MGKLQRVSAQLAELSPEQGAPFQQQCQAAEEQYGSIREHVRQAATVLEDAIPRYSQVHRRMDFLLESLERLQGRVQNPPVVRGDAAQLREQICENSLALGELEKLGVALETVRSQGAELLASKQMPLIAVSCLAVIQERTEQLCSQWRCLCGQAEERERWLRGLLALAERFWQGLAELAVSLTDTQQMVLNLEEAGSDPEAALREEIDVLQNDLDTLGILGVELMSSCGDPDKPDVTKSLDDHQLEAALLGLGQFQNQLEELLQWISHTAEQLQGQTPLSLDLQSCEIELAKHKVRSSRERLRLREFTANCSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJS9</accession>
    <name>A0A452IJS9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription factor AP-2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJS9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028069"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031885.1">
        <property type="protein sequence ID" value="ENSGAGP00000028069.1"/>
        <property type="gene ID" value="ENSGAGG00000020390.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004979">
        <property type="entry name" value="TF_AP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008122">
        <property type="entry name" value="TF_AP2_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013854">
        <property type="entry name" value="TF_AP2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812:SF14">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03299">
        <property type="entry name" value="TF_AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01750">
        <property type="entry name" value="AP2BTNSCPFCT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01748">
        <property type="entry name" value="AP2TNSCPFCT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor AP-2 C-terminal" evidence="8">
        <location>
            <begin position="230"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="30"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="436"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="460" mass="50513" checksum="1E096904BF2BC166" modified="2019-05-08" version="1">MHSPPREQPAIMLWKLVENVKYEDIYEDRHDGVPSHSSRLSQLGSVSQGPYSSAPPLSHTPSSDFQPPYFPPPYQPLPYHQSQDPYSHVNDPYSLNPLHQPQQHPWGQRQRQEVGSEAGSLLPQPRAALPQLSGLDPRRDYHSVRRPDVLLHSAHHGLDSGMGDSLSLHGIGHPGMEDVQSVEDANNSGMNLLDQSVIKKVPVPPKSVTSLMMNKDGFLGGMSVNTGEVFCSVPGRLSLLSSTSKYKVTVGEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLRERLEKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYICETEFPAKAVSEYLNRQHTDPSDLHSRKNMLLATKQLCKEFTDLLAQDRTPIGNSRPSPILEPGIQSCLTHFSLITHGFGAPAICAALTALQNYLTEALKGMDKMFLNNNTANRHTSGEGPGSKTGDKEEKHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IL21</accession>
    <name>A0A452IL21_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IL21"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028716"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032625.1">
        <property type="protein sequence ID" value="ENSGAGP00000028716.1"/>
        <property type="gene ID" value="ENSGAGG00000020815.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051059">
        <property type="term" value="F:NF-kappaB binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033209">
        <property type="term" value="P:tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047155">
        <property type="entry name" value="COMMD4/6/7/8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 4-8 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231:SF2">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="1">
        <location>
            <begin position="61"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="128" mass="14628" checksum="B03CB2CC59A27D1D" modified="2019-05-08" version="1">FLPLEFFFLGALKRNLSSEQVRADFIALGSWLGVHFLLLQWKLNSPTLTRLAIGQTLMINQLIDMEWKFGVTAGSSELEKVGSIFLQLKLVVKKGSQMDHIYVELTLPQFYSFLHEMGRVKTSLECFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILP7</accession>
    <name>A0A452ILP7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytoplasmic dynein 1 intermediate chain 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Cytoplasmic dynein intermediate chain 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Dynein intermediate chain 1, cytosolic</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCTN1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILP7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028773"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032688.1">
        <property type="protein sequence ID" value="ENSGAGP00000028773.1"/>
        <property type="gene ID" value="ENSGAGG00000018245.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000108">
        <property type="entry name" value="cytoplasmic dynein 1 intermediate chain 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000026">
        <property type="entry name" value="cytoplasmic dynein 1 intermediate chain 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025956">
        <property type="entry name" value="DYNC1I1/DYNC1I2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF34">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11540">
        <property type="entry name" value="Dynein_IC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="96"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="193"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="20"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00081333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="645" mass="72953" checksum="C213E70A286F52EA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILY4</accession>
    <name>A0A452ILY4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Fibrinogen C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Homotetramer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILY4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029051"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033003.1">
        <property type="protein sequence ID" value="ENSGAGP00000029051.1"/>
        <property type="gene ID" value="ENSGAGG00000021023.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF45">
        <property type="entry name" value="FIBRINOGEN C DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="14">
        <location>
            <begin position="234"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="460" mass="51423" checksum="D366150F4CBA11C6" modified="2019-05-08" version="1">MVNDRWKDMGRASPLEDGQQEKSQRMSCGYILCTVLLSVAVLLAVTVTGAILFMNHYHTPVTESPPIISTNPEEANALVTIEKVDSSRINIFIDPNCPDQAGSFTRLEGFQSSVLRAVIDHDTEMKSSKGQERALLVTVADEVAKLLTHATQLRADCDSLKKGHNAMGQELNALQNEQGRLIQLLSESQTNMARLVSSVSDVLDTLLKDRGMARPRLKADLQRPPARGARPRGCSNGSRPRDCFDIYTSGQQEDGIYSIFPTHYPSGFQVYCDMTTDGGGWTVFQRREDGSVNFFRGWEAYRDGFGKLTGEHWLGLARLHALTTQASYELRIDLEDFDNGTAFAQYGTFGVGLFSVDPEEDGYPVTIADYSGTAGDSFLKHNGMKFTTKDLDNDHSENNCAAFYHGAWWYRNCHTSNLNGQYLKGHHSSYADGVEWSSWTGWQYSLKFTEMKTRPIREEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IM33</accession>
    <name>A0A452IM33_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452IM33"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028912"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032842.1">
        <property type="protein sequence ID" value="ENSGAGP00000028912.1"/>
        <property type="gene ID" value="ENSGAGG00000020941.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06655">
        <property type="entry name" value="STKc_PAK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000069">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000001">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035064">
        <property type="entry name" value="STK_PAK2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF21">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="12">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="248"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="141"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="523" mass="57791" checksum="E664A761DBC7253E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IP37</accession>
    <name>A0A452IP37_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ETS proto-oncogene 1, transcription factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IP37"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029609"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033622.1">
        <property type="protein sequence ID" value="ENSGAGP00000029609.1"/>
        <property type="gene ID" value="ENSGAGG00000021383.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08542">
        <property type="entry name" value="SAM_PNT-ETS-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000014">
        <property type="entry name" value="Protein c-ets-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045688">
        <property type="entry name" value="Ets1_N_flank"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003118">
        <property type="entry name" value="Pointed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041886">
        <property type="entry name" value="SAM_PNT-ETS-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF209">
        <property type="entry name" value="ETS TRANSLOCATION VARIANT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19525">
        <property type="entry name" value="Ets1_N_flank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02198">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00251">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51433">
        <property type="entry name" value="PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNT" evidence="5">
        <location>
            <begin position="94"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="ETS" evidence="4">
        <location>
            <begin position="378"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="433" mass="49315" checksum="87893250FAEB2A61" modified="2019-05-08" version="1">MMSYYMDTTIGSPAPYPLARPGVMGAANPYEDPWMSCGFQSTCYQQRICYPVWDESTIQEVPTGIERYNSDMECADVPLLTPSSKEMMSQALKATFSGFTKEQQRLGIPKDPQQWTETHVRDWVMWAVNEFSLKDVDFQKFCMNGAALCALGKDCFLELAPDFVGDILWEHLEILQKDEIKPYSANGVNPAYPESRYTSDYFISYGIEHAQCVPPSEFSEPSFITESYQTLHPISSEELFSLKYENDYPSVILRDPVQTDSLQTDYFTIKQEVVTPDNMCMGRTSRGKLGGQDSFESIESYDSCDRLTQSWNSQSSFQSLQRVPSYDSFDSEDYPAALPNHKPKGTFKDYVRDRANMNKDKPVIPAAALAGYTGSGPIQLWQFLLELLTDKSCQSFISWTGDGWEFKLSDPDEVWPIVTDQRPLKMLRRIPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRQ6</accession>
    <name>A0A452IRQ6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IRQ6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030801"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034930.1">
        <property type="protein sequence ID" value="ENSGAGP00000030801.1"/>
        <property type="gene ID" value="ENSGAGG00000022145.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15232">
        <property type="entry name" value="7tmA_OR13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="198"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="308" mass="34823" checksum="96E5D5869EF3765E" modified="2019-05-08" version="1">MLNHTRVTEFIMQGLFDHPHHQGLFFGLFLCLYTAVIMGNSLIIVATLLHPPLHTPMYFFIANLATLDIMCSSSVLPKMMKNLMQKKKTISFDGCMAQVFAFTLSGGTELMLLTIMSYDRYVAICHPLLYVNLMTKRICISLAIGVWAVGVVNSVVNTLLMLLLDFCGPNLIQHFFCEIPPVLALSCSSIYLNQVMMFLADIFLAMGNFLLTTVSYSFIIITILKIRSSKGKQRAFSTCSSHLLVVTLFYSSIIYTYIRPASSYSLDKDKLVAIMYTLMTPILNPLIYSLRNRDIKIAFRKIFSFAMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IT11</accession>
    <name>A0A452IT11_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sorting nexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IT11"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031262"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035459.1">
        <property type="protein sequence ID" value="ENSGAGP00000031262.1"/>
        <property type="gene ID" value="ENSGAGG00000022469.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036089">
        <property type="term" value="P:cleavage furrow formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07669">
        <property type="entry name" value="BAR_SNX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11896">
        <property type="entry name" value="SH3_SNX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000033">
        <property type="entry name" value="Sorting nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000116">
        <property type="entry name" value="Sorting nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000004">
        <property type="entry name" value="Sorting nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037427">
        <property type="entry name" value="SNX33_BAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014536">
        <property type="entry name" value="Snx9_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019497">
        <property type="entry name" value="Sorting_nexin_WASP-bd-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45827">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45827:SF3">
        <property type="entry name" value="SORTING NEXIN-33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10456">
        <property type="entry name" value="BAR_3_WASP_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027744">
        <property type="entry name" value="Snx9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="11 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="17">
        <location>
            <begin position="218"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="64"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR027744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR027744-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="562" mass="64651" checksum="867F7C910D263BBB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWT2</accession>
    <name>A0A452IWT2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IWT2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032551"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036899.1">
        <property type="protein sequence ID" value="ENSGAGP00000032551.1"/>
        <property type="gene ID" value="ENSGAGG00000023282.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000155">
        <property type="entry name" value="membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316">
        <property type="entry name" value="MEMBRANE ASSOCIATED GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316:SF12">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE, WW AND PDZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="17"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="145" mass="14726" checksum="D74BC59F902830FB" modified="2019-05-08" version="1">MSKVIQKKNHWSSRVHQCSARRAAGGQPGLPLLGGAEHGQFPYLGAAEEGGPPVLAGGEEGGLSPGELLLEVQGVRVSGLPRYDVLEVIRSCTEPIAVKAVRQGKGAARGSRGALGGSAVIALAVPLDCPGLSSSLPRAGWALAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IX00</accession>
    <name>A0A452IX00_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCAAT/enhancer-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the bZIP family. C/EBP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IX00"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032656"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037012.1">
        <property type="protein sequence ID" value="ENSGAGP00000032656.1"/>
        <property type="gene ID" value="ENSGAGG00000023346.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030099">
        <property type="term" value="P:myeloid cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14711">
        <property type="entry name" value="bZIP_CEBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000028">
        <property type="entry name" value="CCAAT/enhancer-binding protein beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031106">
        <property type="entry name" value="C/EBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016468">
        <property type="entry name" value="C/EBP_chordates"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334">
        <property type="entry name" value="CCAAT/ENHANCER BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334:SF5">
        <property type="entry name" value="CCAAT_ENHANCER-BINDING PROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005879">
        <property type="entry name" value="CCAAT/enhancer-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="10">
        <location>
            <begin position="248"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="196"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="324" mass="35770" checksum="1F8416D6802D8C1D" modified="2019-05-08" version="1">MEQANFYEVDSRPPMSSSSSHLPTPQPGSAYSYREAPSAATPAAGGAELSDICENENSIDISAYIDPAAFNDEFLADLFQHSKQQEKAKAILAGDFDFHSMHGASAAALGQGHQPPQQHHPQQLFGCMAGYTDSKLDPLYERIAAPGLRPLVIKQEPREEAEGKQAALAALYPHLPQQQHPSHLQYQIAHCAQTTMHLQPGHPTPPPTPVPSPHHPHHPSSLPAATGALKMMPADHRGKTKKSVDKSSSEYRVRRERNNIAVRKSRDKAKQRNAETQQKVMELTSDNDRLRKRVEQLTRELETLRGIFRQLPESSLVKAMGNCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYN7</accession>
    <name>A0A452IYN7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Unk like zinc finger</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unkempt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYN7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033173"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037597.1">
        <property type="protein sequence ID" value="ENSGAGP00000033173.1"/>
        <property type="gene ID" value="ENSGAGG00000023664.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040594">
        <property type="entry name" value="UNK_Znf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057295">
        <property type="entry name" value="UNK_Znf_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057296">
        <property type="entry name" value="UNK_Znf_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045234">
        <property type="entry name" value="Unkempt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493:SF37">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UNKL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493">
        <property type="entry name" value="UNKEMPT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23261">
        <property type="entry name" value="zf-CCCH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25427">
        <property type="entry name" value="zf-CCCH_UNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23035">
        <property type="entry name" value="zf-CCCH_UNK-like_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18384">
        <property type="entry name" value="zf_CCCH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="76"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="116"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="244"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="286"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="779"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="76"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="116"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="244"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="286"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="405"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="584"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="658"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="405"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="588"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="830" mass="91944" checksum="A7A1C23ED6E12C7D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0B6</accession>
    <name>A0A452J0B6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Integrin alpha-V</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Vitronectin receptor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 21">Membrane</location>
            <topology evidence="2 21">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J0B6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033801"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038288.1">
        <property type="protein sequence ID" value="ENSGAGP00000033801.1"/>
        <property type="gene ID" value="ENSGAGG00000023825.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.130:FF:000003">
        <property type="entry name" value="Integrin alpha V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1510:FF:000001">
        <property type="entry name" value="Integrin alpha V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1530:FF:000002">
        <property type="entry name" value="integrin alpha-V isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.930:FF:000001">
        <property type="entry name" value="Integrin subunit alpha V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1460:FF:000001">
        <property type="entry name" value="Integrin, alpha V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF4">
        <property type="entry name" value="INTEGRIN ALPHA-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00357">
        <property type="entry name" value="Integrin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 21" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 21" id="KW-0401">Integrin</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018816708" description="Integrin alpha-V" evidence="21">
        <location>
            <begin position="21"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="986"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="20">
        <location>
            <begin position="22"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="20">
        <location>
            <begin position="98"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="20">
        <location>
            <begin position="222"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="20">
        <location>
            <begin position="277"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="20">
        <location>
            <begin position="343"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="20">
        <location>
            <begin position="404"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="23">
        <location>
            <begin position="452"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="24">
        <location>
            <begin position="611"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="25">
        <location>
            <begin position="764"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1019"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1019"/>
            <end position="1034"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00082120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1040" mass="115082" checksum="FB62B9BD17016D03" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0E6</accession>
    <name>A0A452J0E6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PA-PLA1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J0E6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033834"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038320.1">
        <property type="protein sequence ID" value="ENSGAGP00000033831.1"/>
        <property type="gene ID" value="ENSGAGG00000024071.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000038323.1">
        <property type="protein sequence ID" value="ENSGAGP00000033834.1"/>
        <property type="gene ID" value="ENSGAGG00000024071.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000038329.1">
        <property type="protein sequence ID" value="ENSGAGP00000033840.1"/>
        <property type="gene ID" value="ENSGAGG00000024071.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000034">
        <property type="entry name" value="ankyrin repeat and SAM domain-containing protein 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004177">
        <property type="entry name" value="DDHD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058055">
        <property type="entry name" value="PA-PLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057825">
        <property type="entry name" value="WWE_SEC23-DDH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509">
        <property type="entry name" value="PA-PL1 PHOSPHOLIPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509:SF7">
        <property type="entry name" value="PHOSPHOLIPASE DDHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02862">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02825">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23464">
        <property type="entry name" value="WWE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01127">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51043">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WWE" evidence="3">
        <location>
            <begin position="25"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="DDHD" evidence="4">
        <location>
            <begin position="504"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="440"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="628"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="468"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="628"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="719" mass="81787" checksum="49D3EC5C15249F35" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1V1</accession>
    <name>A0A452J1V1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J1V1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034367"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038910.1">
        <property type="protein sequence ID" value="ENSGAGP00000034367.1"/>
        <property type="gene ID" value="ENSGAGG00000024422.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042327">
        <property type="term" value="P:positive regulation of phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039886">
        <property type="entry name" value="BTBD10/KCTD20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21637">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21637:SF1">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="173" mass="20114" checksum="8DB5FBBA21622BD6" modified="2019-05-08" version="1">MVGSARKGERECHIVVLTDEDTVDWDEDHPPPMGEEYSQILYSSKLYRFFKYIENRDVAKTVLKERGLKNIRIGIEGYPTCKEKVKRRPGGRSEVIYNYVQRPFIQMSWEKEEGKSRHVDFQCVRSKSLTNLVAVGEDVSEDQELLMHHPPQVDELDRLNAPYSQMAPNDLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J3M6</accession>
    <name>A0A452J3M6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-threonine 3-dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.1.1.103</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate, mediating L-threonine catabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-threonine + NAD(+) = (2S)-2-amino-3-oxobutanoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13161"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78948"/>
            <dbReference type="EC" id="1.1.1.103"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.103" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452J3M6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034921"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039545.1">
        <property type="protein sequence ID" value="ENSGAGP00000034921.1"/>
        <property type="gene ID" value="ENSGAGG00000024830.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008743">
        <property type="term" value="F:L-threonine 3-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05272">
        <property type="entry name" value="TDH_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000077">
        <property type="entry name" value="L-threonine 3-dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051225">
        <property type="entry name" value="NAD(P)_epim/dehydratase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42687">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42687:SF1">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="7">
        <location>
            <begin position="58"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00050613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="328" mass="36232" checksum="D76991E5EAEBD80C" modified="2019-05-08" version="1">MAIVKNLGRTLSQLLQSPGCGCQTSIVPVRCIGVSPRQVTSDASFHSVSFSETDHPRVLITGGLGQLGVGLAKLLRKRFGRNNVILSDIRKPPDDVFYNGPFIYSDILDYKNLREIVVNNRITWLFHYSALLSAVGEANVPLARAVNITGLHNILDIAAEHSLRLFVPSTIGAFGPTSPRNPAPDLCIQRPRTIYGVSKVHAELMGEYYHYRYGLDFRCLRYPGIISADSQPGGGTTDYAVQIFHDAIKNGKFQCNLKSDTRLPMMYIDDCLKATLDVMEAPAASLSMRTYNISAMSFSPEELVQEVKKHIPELQVTYTVDAVRQAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4W6</accession>
    <name>A0A452J4W6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">U1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000035522"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040230.1">
        <property type="protein sequence ID" value="ENSGAGP00000035522.1"/>
        <property type="gene ID" value="ENSGAGG00000025253.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000680">
        <property type="entry name" value="S phase cyclin A-associated protein in the endoplasmic reticulum"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032446">
        <property type="entry name" value="SCAPER_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31434">
        <property type="entry name" value="S PHASE CYCLIN A-ASSOCIATED PROTEIN IN THE ENDOPLASMIC RETICULUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31434:SF2">
        <property type="entry name" value="S PHASE CYCLIN A-ASSOCIATED PROTEIN IN THE ENDOPLASMIC RETICULUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16501">
        <property type="entry name" value="SCAPER_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U1-type" evidence="3">
        <location>
            <begin position="836"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="288"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="340"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="565"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="748"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="465"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="297"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="586"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1446" mass="164377" checksum="566BD856AE70849D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFF0</accession>
    <name>A0A452GFF0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GFF0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000268"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000304.1">
        <property type="protein sequence ID" value="ENSGAGP00000000268.1"/>
        <property type="gene ID" value="ENSGAGG00000000238.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="52515" checksum="E1213BC7EBD9500F" modified="2019-05-08" version="1">MQGTAFSRMEWKSINLMKKLAQIQTDIIFLSKCKRMDIIPNGLKVKNPLLSTYYTDHSERLCHTLSKKLRNHLISILYSKQENIKKELSNLETLINNQTSIQTDFTRIRQEIYITHFTSLQRKKDCKLSKLLPATWGHNSGTPNPPSNIVNLSNYTLSPEEKSVLSRGLSFCPATPTNMIQFCGDLEAYFRRLRLKEYFQDNTEQCTDTRVPSHQQHKKKNSTWTPPEGRNDSLDLYIECFRRRAQAEIVEQQHRLPHNLSRAERNAIHSLRNHPDIIIKEADKGGAVVIMNRSDYQKEAARQLSNTKFYRPLPSDPTEEYTKKLQHLLRTLPTLTPEEINIPLEPRPGLFYLLPKIHKPGNPGRPIISGIGTLTEGLSGYMDSLLRPYATSTPSYLRDTTDFLRKLQCIGDLPENTILATMDVEALYTNIPHTDGIQAVRNTIPDDATAQLAAELCAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GG01</accession>
    <name>A0A452GG01_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GG01"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000427"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000480.1">
        <property type="protein sequence ID" value="ENSGAGP00000000427.1"/>
        <property type="gene ID" value="ENSGAGG00000000328.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="87" mass="10266" checksum="53DDB84A63E7442D" modified="2019-05-08" version="1">MLARMETTLQKMEMPKQTRHRKLKVLVMPSELCEFLITVYFCFITMQNFVLLFFKLFPLVYFVLGGFPDAAYKFRFVKCIGVNIEGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GI57</accession>
    <name>A0A452GI57_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Synaptotagmin-like mitochondrial and lipid-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GI57"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001324"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001484.1">
        <property type="protein sequence ID" value="ENSGAGP00000001324.1"/>
        <property type="gene ID" value="ENSGAGG00000001064.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000001495.1">
        <property type="protein sequence ID" value="ENSGAGP00000001335.1"/>
        <property type="gene ID" value="ENSGAGG00000001064.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098592">
        <property type="term" value="C:cytoplasmic side of apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035774">
        <property type="term" value="P:positive regulation of insulin secretion involved in cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21683">
        <property type="entry name" value="SMP_C2CD2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039934">
        <property type="entry name" value="C2CD2/C2CD2L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040885">
        <property type="entry name" value="SMP_C2CD2L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119:SF8">
        <property type="entry name" value="PHOSPHOLIPID TRANSFER PROTEIN C2CD2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18696">
        <property type="entry name" value="SMP_C2CD2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Synaptotagmin-like mitochondrial and lipid-binding" evidence="2">
        <location>
            <begin position="90"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="643"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="685"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="594"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="704" mass="76566" checksum="34DC194044623CB5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GI61</accession>
    <name>A0A452GI61_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytosolic carboxypeptidase 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GI61"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001270"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001425.1">
        <property type="protein sequence ID" value="ENSGAGP00000001270.1"/>
        <property type="gene ID" value="ENSGAGG00000001020.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF9">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="117"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18556" checksum="920C8C113A4706CD" modified="2019-05-08" version="1">MMNGFMYGNIFEEEERFQRQAIFPKLLCQNAEDFSFSSTSFNRDAVKAGTGRRFLGGLLDHSSYCYTLEVSFYSYILGGTTSTVPYTEEAYMKLGRNVARTFLDYYRLNTISERPLAPIPNTRKEKMPPFKSVPQRGPGNSHPGRKLEKRSQTNPMEHSHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJA3</accession>
    <name>A0A452GJA3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Diaphanous related formin 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000001747"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001984.1">
        <property type="protein sequence ID" value="ENSGAGP00000001747.1"/>
        <property type="gene ID" value="ENSGAGG00000001377.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.630:FF:000001">
        <property type="entry name" value="Diaphanous related formin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014767">
        <property type="entry name" value="DAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010465">
        <property type="entry name" value="Drf_DAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051412">
        <property type="entry name" value="Formin_Homology_Diaphanous_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691:SF9">
        <property type="entry name" value="PROTEIN DIAPHANOUS HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06345">
        <property type="entry name" value="Drf_DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51231">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="4">
        <location>
            <begin position="87"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="DAD" evidence="3">
        <location>
            <begin position="509"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="482"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="564" mass="65065" checksum="BFF638276DDF5247" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJA6</accession>
    <name>A0A452GJA6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">cAMP-dependent protein kinase catalytic subunit alpha</fullName>
            <ecNumber evidence="6">2.7.11.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.11" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452GJA6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001758"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002000.1">
        <property type="protein sequence ID" value="ENSGAGP00000001758.1"/>
        <property type="gene ID" value="ENSGAGG00000001402.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14209">
        <property type="entry name" value="STKc_PKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044109">
        <property type="entry name" value="STKc_PKA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF82">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0114">cAMP</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0519">Myristate</keyword>
    <keyword evidence="14 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="58"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="25">
        <location>
            <begin position="287"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="339" mass="38997" checksum="660BBE7FC575AA66" modified="2019-05-08" version="1">RGISRLSVHQAGCPTPPDSLRCSLSVSLTVKEFLAKAKEDFLKKWENPSQNTASLDQFERIKTLGTGSFGRVLLVKHKETGNYYAMKVLEKQKVVKLKQIEHTLNEKRILQAINFPLLIKLEYSFKDNSNLYMVMEYIPGGEMFSHLQRIGRFSEPHARFYAAQIQGYIEVMDFGFDKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFVPKCKGPGDTSNFDDYEEEEIRVSTTEECAMEFAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMH8</accession>
    <name>A0A452GMH8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMH8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003023"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003463.1">
        <property type="protein sequence ID" value="ENSGAGP00000003023.1"/>
        <property type="gene ID" value="ENSGAGG00000002415.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000003471.1">
        <property type="protein sequence ID" value="ENSGAGP00000003031.1"/>
        <property type="gene ID" value="ENSGAGG00000002415.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050900">
        <property type="term" value="P:leukocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.130:FF:000007">
        <property type="entry name" value="Integrin subunit alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.930:FF:000001">
        <property type="entry name" value="Integrin subunit alpha V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF89">
        <property type="entry name" value="INTEGRIN ALPHA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0401">Integrin</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044516209" evidence="14">
        <location>
            <begin position="37"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="994"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="13">
        <location>
            <begin position="43"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="13">
        <location>
            <begin position="296"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="13">
        <location>
            <begin position="358"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="13">
        <location>
            <begin position="417"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="15">
        <location>
            <begin position="464"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="16">
        <location>
            <begin position="616"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="17">
        <location>
            <begin position="770"/>
            <end position="980"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1053" mass="115905" checksum="B2435CA2E4B40548" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNQ8</accession>
    <name>A0A452GNQ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GNQ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003516"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004054.1">
        <property type="protein sequence ID" value="ENSGAGP00000003516.1"/>
        <property type="gene ID" value="ENSGAGG00000002843.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042613">
        <property type="term" value="C:MHC class II protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002504">
        <property type="term" value="P:antigen processing and presentation of peptide or polysaccharide antigen via MHC class II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05766">
        <property type="entry name" value="IgC1_MHC_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000116">
        <property type="entry name" value="HLA class II histocompatibility antigen, DRB1-1 beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.320.10">
        <property type="entry name" value="Class II Histocompatibility Antigen, M Beta Chain, Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014745">
        <property type="entry name" value="MHC_II_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000353">
        <property type="entry name" value="MHC_II_b_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF99">
        <property type="entry name" value="HLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DRB1 BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00969">
        <property type="entry name" value="MHC_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00921">
        <property type="entry name" value="MHC_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0491">MHC II</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019475397" description="Ig-like domain-containing protein" evidence="11">
        <location>
            <begin position="49"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="147"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="302" mass="33808" checksum="5B497FA50524E44B" modified="2019-05-08" version="1">MGLCCLLPLCPVLTGEAGSGSRRGRSTCSAQSWGNLTLVLSLFPGVLSSDVKASREHFLLQEKSECHYTNGMQQVRYLKRLIWGQQEICYYDSDLGFSVARTELGRPIAEYWNRLEWLSYLWASVEKFCSYNHRRFENITVGRQVKPRVKISTLKAESSHHPSLLVCAATGFYPSEIATKWLKNGQEETAGVVSRELLHNGDWTFQIQVTLETKPRWGDVYTCQVEHVSLQTPITMQWVAQSDSAKSKMFVGIGGFVLGLIFMVLGLLVYQKNKKGYLCLDTADSQAAGSDRGSTTNLLLCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNT8</accession>
    <name>A0A452GNT8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dUTPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GNT8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003340"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003848.1">
        <property type="protein sequence ID" value="ENSGAGP00000003340.1"/>
        <property type="gene ID" value="ENSGAGG00000002697.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051592">
        <property type="entry name" value="HERV-K_Pro_peptidase_A2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19422">
        <property type="entry name" value="GAG RETROVIRAL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19422:SF123">
        <property type="entry name" value="RT1 CLASS I, LOCUS CE15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase-like" evidence="4">
        <location>
            <begin position="14"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="164" mass="17339" checksum="8EC3944BBCD4020A" modified="2019-05-08" version="1">MPLEWARLIRCGGATRGSAGVDLATAADVTLADDKVQIVPSVVNGPLGYGLSALLIGRSSMSKQGLFVLPGLIDVDYTGNIGIMVRALFPPVNIAAGTRIAQLIPFQSSVPKTKLTERGSHGFGSTDSPQVAFAMTVTQRKPSRMVPGQNSTTGTLFLFRHAHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GP97</accession>
    <name>A0A452GP97_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">11-beta-hydroxysteroid dehydrogenase 1</fullName>
            <ecNumber evidence="9">1.1.1.146</ecNumber>
            <ecNumber evidence="10">1.1.1.201</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">7-oxosteroid reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Corticosteroid 11-beta-dehydrogenase isozyme 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>3beta,7alpha-dihydroxyandrost-5-en-17-one + NADP(+) = 3beta-hydroxy-5-androstene-7,17-dione + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69440"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:81471"/>
            <dbReference type="ChEBI" id="CHEBI:183808"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:69441"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3beta-hydroxy-5-androstene-7,17-dione + NADPH + H(+) = 3beta,7beta-dihydroxyandrost-5-en-17-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:69452"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:183368"/>
            <dbReference type="ChEBI" id="CHEBI:183808"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:69453"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>3beta-hydroxy-5alpha-androstane-7,17-dione + NADPH + H(+) = 3beta,7beta-dihydroxy-5alpha-androstan-17-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:69456"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:79834"/>
            <dbReference type="ChEBI" id="CHEBI:183809"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:69457"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>7-oxocholesterol + NADPH + H(+) = 7beta-hydroxycholesterol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68656"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:42989"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:64294"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:68657"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>7-oxolithocholate + NADPH + H(+) = ursodeoxycholate + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:47540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78604"/>
            <dbReference type="ChEBI" id="CHEBI:78605"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:47541"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>7-oxopregnenolone + NADPH + H(+) = 7beta-hydroxypregnenolone + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:69436"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:183806"/>
            <dbReference type="ChEBI" id="CHEBI:183807"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:69437"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a 7beta-hydroxysteroid + NADP(+) = a 7-oxosteroid + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20233"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:35349"/>
            <dbReference type="ChEBI" id="CHEBI:47789"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.201"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="25">
            <dbReference type="Rhea" id="RHEA:20235"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>an 11beta-hydroxysteroid + NADP(+) = an 11-oxosteroid + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11388"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:35346"/>
            <dbReference type="ChEBI" id="CHEBI:47787"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.146"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:11389"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:11390"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>chenodeoxycholate + NADP(+) = 7-oxolithocholate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53820"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36234"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78605"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:53822"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>corticosterone + NADP(+) = 11-dehydrocorticosterone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42200"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16827"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78600"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:42201"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:42202"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>glycochenodeoxycholate + NADP(+) = 7-oxoglycolithocholate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:65056"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36252"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:137818"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:65058"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>glycoursodeoxycholate + NADP(+) = 7-oxoglycolithocholate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68976"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:132030"/>
            <dbReference type="ChEBI" id="CHEBI:137818"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:68978"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>taurochenodeoxycholate + NADP(+) = 7-oxotaurolithocholate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:65060"/>
            <dbReference type="ChEBI" id="CHEBI:9407"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:137724"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:65062"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>tauroursodeoxycholate + NADP(+) = 7-oxotaurolithocholate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68980"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:132028"/>
            <dbReference type="ChEBI" id="CHEBI:137724"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="24">
            <dbReference type="Rhea" id="RHEA:68982"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.146" evidence="9"/>
    <dbReference type="EC" id="1.1.1.201" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452GP97"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003716"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004297.1">
        <property type="protein sequence ID" value="ENSGAGP00000003716.1"/>
        <property type="gene ID" value="ENSGAGG00000003016.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070524">
        <property type="term" value="F:11-beta-hydroxysteroid dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047022">
        <property type="term" value="F:7-beta-hydroxysteroid dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005496">
        <property type="term" value="F:steroid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006706">
        <property type="term" value="P:steroid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051253">
        <property type="entry name" value="11-beta-HSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44279:SF1">
        <property type="entry name" value="11-BETA-HYDROXYSTEROID DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44279">
        <property type="entry name" value="HYDROXYSTEROID (11-BETA) DEHYDROGENASE 1-LIKE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00049300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00049520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="286" mass="31492" checksum="BBB3FEE2EE04EDD6" modified="2019-05-08" version="1">PGYLDCKVFGSRGGLAVCGFFWVQHFMLKGKRVIVTGASTGIGEQMAYHLARMGAHVLVTARTEAKLQKVCTKHTCTGQSYVTLCPANGPQSCPAEPPMLFKTWTHTHRGLDMLILNHIGNSYFNYFDGDVGHVQKLLKINFLSYVAMTVSALPRLKDSGGSIVVVSSMAGKAGFPFTAPYSATKFALDGFFSSLRQELIIQKVDVSITLCILGFIDTAASDVVLVSPAPKEECALEIIKGGALRQREIYYKYAATKIPLLIRDWAPELLDYLIRRSYNVENLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPQ4</accession>
    <name>A0A452GPQ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPQ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003884"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004509.1">
        <property type="protein sequence ID" value="ENSGAGP00000003884.1"/>
        <property type="gene ID" value="ENSGAGG00000003179.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030990">
        <property type="term" value="C:intraciliary transport particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028172">
        <property type="entry name" value="FT20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31978">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31978:SF1">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14931">
        <property type="entry name" value="IFT20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="79"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="132" mass="15313" checksum="851CFB0F1C12AC45" modified="2019-05-08" version="1">MAKGILGDARLHFDELNKLRILDPEVAQQTTELKEECKAFVDKIAEFQKIVGSLIELVDQLAKATENEKMKAIGARNLLKSIAKQREAQQQQLQALITEKKMQLERYRVEYETLCKIEADQNEFIDQFIFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GR23</accession>
    <name>A0A452GR23_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GR23"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004399"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005163.1">
        <property type="protein sequence ID" value="ENSGAGP00000004399.1"/>
        <property type="gene ID" value="ENSGAGG00000003646.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034046">
        <property type="term" value="F:poly(G) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12758">
        <property type="entry name" value="RRM1_hnRPDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12585">
        <property type="entry name" value="RRM2_hnRPDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000220">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein D-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000030">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein d0 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034847">
        <property type="entry name" value="hnRPDL_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033:SF2">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN D-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="30"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="115"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="193"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="272"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="302" mass="33666" checksum="40D6CFD41D6DE2B3" modified="2019-05-08" version="1">MEDMTEYSGGLEEFAEGSKINASKNQQDDGKMFIGGLSWDTSKKDLTEYLSRFGEVVDCTIKTDPVTGRSRGFGFVLFKDAASVDKVLELKEHKLDGKLIDPKRAKALKGKEPPKKVFVGGLSPDTSEEQIKEYFGAFGEIENIELPMDTKTNERRGFCFITYTDEEPVKKLLESRYHQIGSGKCEIKVAQPKEVYRQQQQQQQKGGRGTSSGGRGGGRGRGRGQGQNWNQGFNNYYDQGYGNYNSTYSDPSYSGYGGYDYSGYNYPSYGYGQGYADYSGQQSTYGKASRGGGNHQNNYQPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GS49</accession>
    <name>A0A452GS49_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein tweety homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Probable chloride channel.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-glutamate(out) = L-glutamate(in)</text>
            <dbReference type="Rhea" id="RHEA:66336"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:66338"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homotetramer; disulfide-linked. Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the tweety family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GS49"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005310.1">
        <property type="protein sequence ID" value="ENSGAGP00000004534.1"/>
        <property type="gene ID" value="ENSGAGG00000003727.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030868">
        <property type="term" value="C:smooth endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005229">
        <property type="term" value="F:intracellularly calcium-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072320">
        <property type="term" value="F:volume-sensitive chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006990">
        <property type="entry name" value="Tweety"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424:SF5">
        <property type="entry name" value="PROTEIN TWEETY HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424">
        <property type="entry name" value="TWEETY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04906">
        <property type="entry name" value="Tweety"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 17" id="KW-0868">Chloride</keyword>
    <keyword evidence="10 17" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="342" mass="35504" checksum="47FAABC6DA135A2E" modified="2019-05-08" version="1">QPRSDLAGIGIGFYGNSETNDGVGQATAALLDANLTLQGADVLTLGLLSGAVGGDLTRLEEALAPRIEFVAVVRNTRRQAEAVAQILVGLPFWGALGRSPSQLAHGLAFLEDYRMTVMSFFVLILSWGSLGLEMAAAVGLSDFCSDPDGFVLNLTQAKTELSPEILQYYLTCSQDVPNPFQQRLTMSQRALSSIHSQLHGLEREAIPQFPAAERNLVSVQGTLNSTESNFHQLVALLNCRGLHKVSAPAPPGGVPRLGPPLVGSPPAMVAPLSCGSSQAPPPLSCGSSPQVLPHGAVVASPGSTSVPTPAPGCGSFPKLHSCTPLPLPGCGSSPQAPPPGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GST3</accession>
    <name>A0A452GST3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphopantothenoylcysteine decarboxylase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">CoaC</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the decarboxylation of the cysteine moiety of 4-phosphopantothenoylcysteine to form 4'-phosphopantotheine and this reaction forms part of the biosynthesis of coenzyme A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GST3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004960"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005796.1">
        <property type="protein sequence ID" value="ENSGAGP00000004960.1"/>
        <property type="gene ID" value="ENSGAGG00000004060.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1950:FF:000004">
        <property type="entry name" value="Phosphopantothenoylcysteine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00070201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00082063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="234" mass="26180" checksum="0B6B9A4387B26FBA" modified="2019-05-08" version="1">MEPLTLSEPQPSPQNSLPTPEKTYHVLVGVTGSVAALKLPVLVSELLEIPGLKVRVVTTERAKHFYNPQEIPVTLYSDAEEWQLWKGRTDPVLHIDLRRWADLMLVAPLDANTLAKIANGICDNLLTCVIRAWDMTKPLLFCPAMNTAMWEHPITAHQVEQLKDFGYTEIPCVVKRLVCGDEGVEMQTSNINQKEPLAVPGSMLQVLESTRLATLESLGQLFPRDFNAPFEIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GU61</accession>
    <name>A0A452GU61_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tetratricopeptide repeat protein 17</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in primary ciliogenesis by modulating actin polymerization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TTC17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GU61"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005599"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006526.1">
        <property type="protein sequence ID" value="ENSGAGP00000005599.1"/>
        <property type="gene ID" value="ENSGAGG00000004548.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000053">
        <property type="entry name" value="Tetratricopeptide repeat domain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000061">
        <property type="entry name" value="Tetratricopeptide repeat domain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000111">
        <property type="entry name" value="tetratricopeptide repeat protein 17 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052630">
        <property type="entry name" value="TTC17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16091:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16091">
        <property type="entry name" value="TTC17 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="8 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="7 14" id="KW-0802">TPR repeat</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019402355" description="Tetratricopeptide repeat protein 17" evidence="17">
        <location>
            <begin position="37"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="14">
        <location>
            <begin position="293"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="14">
        <location>
            <begin position="690"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="14">
        <location>
            <begin position="1145"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="779"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="339"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="779"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1201" mass="136653" checksum="2AA26E72F45339CB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUB9</accession>
    <name>A0A452GUB9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GUB9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005645"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006576.1">
        <property type="protein sequence ID" value="ENSGAGP00000005645.1"/>
        <property type="gene ID" value="ENSGAGG00000004581.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000006582.1">
        <property type="protein sequence ID" value="ENSGAGP00000005651.1"/>
        <property type="gene ID" value="ENSGAGG00000004581.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000425">
        <property type="entry name" value="Leucine-rich repeat-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF16">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="387" mass="45028" checksum="E94763051798264C" modified="2019-05-08" version="1">MIHLFFLQEKLNVSNMGHQVIIFDISVVRGFWETQVKKYKEIRKKEKERLAQSSLEKIKQEWDFVLECRKKGIPQSAYLKNGFIDTTVKILDKTEKKTQLCKKSTLSDETDKERSKFIFQLSGEHWTELPDSLKEQTHLKEWHICKTQIQTIPAYIALFQELKVLELSNNQITHLPVEIGCLKQLKELNVSFNNLKSIPPELGNCENLEKLDLAGNIELTELPFELSNLKQVTFVDVSTNKFPTIPICILRMSNLHWLDISNNKLKDLPQDIDRLSELQTFLLQKNKLTYLPRALLNMPKLSLLVVSGDDLVEIPTDVCESPTGLKFISLKDNPLETFLYHDTEDNVESERDREQFEKEFMKSYIADIRGRESMPCYTTKVSFTIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVR1</accession>
    <name>A0A452GVR1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Frizzled-4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GVR1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006044"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007040.1">
        <property type="protein sequence ID" value="ENSGAGP00000006044.1"/>
        <property type="gene ID" value="ENSGAGG00000004908.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042813">
        <property type="term" value="F:Wnt receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061299">
        <property type="term" value="P:retina vasculature morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15038">
        <property type="entry name" value="7tmF_FZD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07448">
        <property type="entry name" value="CRD_FZ4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000020">
        <property type="entry name" value="Frizzled class receptor 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000008">
        <property type="entry name" value="Frizzled receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000539">
        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041765">
        <property type="entry name" value="FZ4_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026551">
        <property type="entry name" value="FZD4_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF23">
        <property type="entry name" value="FRIZZLED-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01534">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00489">
        <property type="entry name" value="FRIZZLED"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01330">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0807">Transducer</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019308592" description="Frizzled-4" evidence="18">
        <location>
            <begin position="26"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="293"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="377"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="424"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="467"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="19">
        <location>
            <begin position="29"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="20">
        <location>
            <begin position="208"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="34"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="42"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="79"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="106"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="526" mass="58999" checksum="39E60149B80DB3B2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVV8</accession>
    <name>A0A452GVV8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ADAM metallopeptidase domain 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GVV8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006179"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007188.1">
        <property type="protein sequence ID" value="ENSGAGP00000006179.1"/>
        <property type="gene ID" value="ENSGAGG00000004985.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990913">
        <property type="term" value="C:sperm head plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008584">
        <property type="term" value="P:male gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000002">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018358">
        <property type="entry name" value="Disintegrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF120">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00289">
        <property type="entry name" value="DISINTEGRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00427">
        <property type="entry name" value="DISINTEGRIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="692"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="12">
        <location>
            <begin position="209"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="11">
        <location>
            <begin position="411"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="637"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="469"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="661"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="759" mass="84864" checksum="9BBE3653DA03F7C1" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVZ1</accession>
    <name>A0A452GVZ1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GVZ1"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007225.1">
        <property type="protein sequence ID" value="ENSGAGP00000006215.1"/>
        <property type="gene ID" value="ENSGAGG00000005000.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="190" mass="19914" checksum="5708D44CF9EB3678" modified="2019-05-08" version="1">NLCPFCCLGCMLCIKYRWGHEDGGSGPSQRTRWCGGAMGGSGPSQRTRWCGGAMGGSGPSQRTRWCGGAMGGSGPSQRTRWCGGAMGGSGPSQRTRWCGGAMGGSGPSQRTRWCGGAMGGSTWVDMVTPPGSPLVEKPASRLSPPSRALRPLLPAARDLRLISHLCAASGLCSPMLCWSIHIYLTKFAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWL4</accession>
    <name>A0A452GWL4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative adherens-junction anchoring domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GWL4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006484"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007540.1">
        <property type="protein sequence ID" value="ENSGAGP00000006481.1"/>
        <property type="gene ID" value="ENSGAGG00000005244.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000007544.1">
        <property type="protein sequence ID" value="ENSGAGP00000006484.1"/>
        <property type="gene ID" value="ENSGAGG00000005244.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000007548.1">
        <property type="protein sequence ID" value="ENSGAGP00000006488.1"/>
        <property type="gene ID" value="ENSGAGG00000005244.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032402">
        <property type="entry name" value="AbLIM_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051618">
        <property type="entry name" value="Actin-binding_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213:SF17">
        <property type="entry name" value="DEMATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16182">
        <property type="entry name" value="AbLIM_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative adherens-junction anchoring" evidence="2">
        <location>
            <begin position="9"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="355" mass="39575" checksum="F1729270C8B28935" modified="2019-05-08" version="1">MERLQKQSLTSPGSVCSSRGSSIPGSPSSIVARMDNEVLGYKDLAAIPKDKAILDIERPDLMIYEPHFTYSLMEHVELPRSRERSLSPKSISPPPSPEVIRDWMENKSLGSASQAASRRTSSTARSGVHHFHRPETNTMELNIYKKPPIYKQREPSTGAPQSKHMIEDQIIECSKFPAAQAPDPNQPAKIETDYWPCPPSLAVVEKEWRQRMASKKGMEEEEDLTEEMTNLRELQKQELSKVTSNLGKLILKEEMEKSLPIRRKTRSLPDRTPFHTSLHLGNSRSSTLPAYGRSTLTRLQSAEFSPASSEKGSPGKRSTWHKGQAQGRCVSGLVWSGHSTQRGHSESSASNSCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYG9</accession>
    <name>A0A452GYG9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GYG9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007211"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008340.1">
        <property type="protein sequence ID" value="ENSGAGP00000007211.1"/>
        <property type="gene ID" value="ENSGAGG00000005792.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098850">
        <property type="term" value="C:extrinsic component of synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017158">
        <property type="term" value="P:regulation of calcium ion-dependent exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061669">
        <property type="term" value="P:spontaneous neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043566">
        <property type="entry name" value="Rabphilin/DOC2/Noc2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729:SF1">
        <property type="entry name" value="DOUBLE C2-LIKE DOMAIN-CONTAINING PROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729">
        <property type="entry name" value="RABPHILIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="118" mass="13671" checksum="0A877183EF445103" modified="2019-05-08" version="1">MDVNGYSDPYVKTYLKPDMEKKSKHKTSVKKKTLNPEYNEEFFYEIAQPELAQKSLEITVWDYDIGKSNDFIGGTTLGLGSQGERLRHWLGCLQTPDQRWEQWHTLTNQLPESSAFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZ32</accession>
    <name>A0A452GZ32_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">p53 apoptosis effector related to PMP-22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Keratinocyte-associated protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM47 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZ32"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007371"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008518.1">
        <property type="protein sequence ID" value="ENSGAGP00000007371.1"/>
        <property type="gene ID" value="ENSGAGG00000005920.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000014">
        <property type="entry name" value="p53 apoptosis effector related to PMP-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015664">
        <property type="entry name" value="P53_induced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14399:SF13">
        <property type="entry name" value="P53 APOPTOSIS EFFECTOR RELATED TO PMP22 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14399">
        <property type="entry name" value="P53-INDUCED PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00076751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="189" mass="21297" checksum="28F37B68A850B411" modified="2019-05-08" version="1">MVQYGLDYTRCRWILPLMLGIAVIFGLIALAGRGWLESETLPYQHQASLWESCTKFNGEPDWTCISLMDYAWGRAAAATFLVGYIILVICFALAIIAFAIDTLRFNFIRGIGGLLFVAAVFSVMGLVIYPVKFTNEIDMEGVNMFSWAYGFAWTTTIMEIGLGFFFCCLPNYEDEILGNVKPTYFYTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZS1</accession>
    <name>A0A452GZS1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Myosin VB</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000007698"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008883.1">
        <property type="protein sequence ID" value="ENSGAGP00000007698.1"/>
        <property type="gene ID" value="ENSGAGG00000005542.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15477">
        <property type="entry name" value="Myo5b_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000006">
        <property type="entry name" value="Myosin VA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1590:FF:000003">
        <property type="entry name" value="Myosin-Va isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000001">
        <property type="entry name" value="unconventional myosin-Va"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058662">
        <property type="entry name" value="Myo5a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037990">
        <property type="entry name" value="Myo5b_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF356">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-VB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25966">
        <property type="entry name" value="Myo5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin motor" evidence="13">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="12">
        <location>
            <begin position="1008"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="605"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="659"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="805"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="892"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="614"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1330" mass="155811" checksum="5AA6C1AA142765D9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H048</accession>
    <name>A0A452H048_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H048"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007813"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009007.1">
        <property type="protein sequence ID" value="ENSGAGP00000007813.1"/>
        <property type="gene ID" value="ENSGAGG00000006226.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000009009.1">
        <property type="protein sequence ID" value="ENSGAGP00000007815.1"/>
        <property type="gene ID" value="ENSGAGG00000006226.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="128"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="431" mass="48431" checksum="B0226FA46CF3F3AB" modified="2019-05-08" version="1">MINFCPQCGQKVETAFHFCPVCGNKLPKEDDEEPMQLTPDVSLSSLKALRQNEPVPETRKTVEWISSEKIPASTSTEEEVWSPQAASPSTKVTSKAKFSPRSPRRLKSTSVTPLPEGKILTDQNSKQWKLGKLLNQSVCGLMYEAQSASGACPQKQRYFLKLDAKDGRIYNEQIFLQRAAKKITVDKWKRMHSVPLLGIPNCIGFGLHESNYRFLVFPELGRTVQSILGDGTNLLTEKPVFQIAIRVLDSLEYIHGNEYVHGDVTAENIYVNPDDLAEVTLAGYCFAFRYCPGGKHVAQCQGSRTPHEGTIEFISLDGHRGAGPSRRSDLQSLGYCMVKWLCGFLPWTDDLADVHKVMEQKERYQMDVVGLLRLCFGRKPIPDALHNYLQQAMALAFEEEPDYEALRKLLGKPLEMKRASAYDSVDLKVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0Q8</accession>
    <name>A0A452H0Q8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H0Q8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008024"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009244.1">
        <property type="protein sequence ID" value="ENSGAGP00000008024.1"/>
        <property type="gene ID" value="ENSGAGG00000006383.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF18">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Immunoglobulin V-set" evidence="5">
        <location>
            <begin position="26"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="128" mass="14465" checksum="51017E0A13400712" modified="2025-10-08" version="2">MITAPTGCSLLLQANGKRTYGNSVTQTEGTIKVCEGKPVLLNCTYEMSGSPVPFWYVQYPHEAPHLLLAEYEATDEEERKRRRGFSAKLERNPTSFRLNKNSSEISDSALYFCALGRHLETVRPHFIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1E1</accession>
    <name>A0A452H1E1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H1E1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008291"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009547.1">
        <property type="protein sequence ID" value="ENSGAGP00000008291.1"/>
        <property type="gene ID" value="ENSGAGG00000006581.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001684">
        <property type="entry name" value="zinc finger protein 33B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002004">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001437">
        <property type="entry name" value="Zinc finger protein 594"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="13">
        <location>
            <begin position="88"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="290"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="487"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="515"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="543"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="571"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="599"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="627"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="655"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="683"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="716" mass="82539" checksum="348CC8E3864AE576" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1L7</accession>
    <name>A0A452H1L7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 24</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 24 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H1L7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008285"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009541.1">
        <property type="protein sequence ID" value="ENSGAGP00000008285.1"/>
        <property type="gene ID" value="ENSGAGG00000006579.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021429">
        <property type="entry name" value="Mediator_Med24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12898">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12898:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11277">
        <property type="entry name" value="Med24_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="987" mass="110490" checksum="B117E378FA77BE13" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3K6</accession>
    <name>A0A452H3K6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Lysosome-associated membrane glycoprotein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytolytic granule membrane</location>
            <topology evidence="12">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Late endosome membrane</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Lysosome membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the LAMP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3K6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009226"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010594.1">
        <property type="protein sequence ID" value="ENSGAGP00000009226.1"/>
        <property type="gene ID" value="ENSGAGG00000007291.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072594">
        <property type="term" value="P:establishment of protein localization to organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.110:FF:000001">
        <property type="entry name" value="lysosome-associated membrane glycoprotein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048528">
        <property type="entry name" value="Lamp2-like_luminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018134">
        <property type="entry name" value="LAMP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002000">
        <property type="entry name" value="Lysosome-assoc_membr_glycop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11506">
        <property type="entry name" value="LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11506:SF27">
        <property type="entry name" value="LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01299">
        <property type="entry name" value="Lamp2-like_luminal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00336">
        <property type="entry name" value="LYSASSOCTDMP"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00310">
        <property type="entry name" value="LAMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51407">
        <property type="entry name" value="LAMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019376213" description="Lysosome-associated membrane glycoprotein 1" evidence="16">
        <location>
            <begin position="27"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Lysosome-associated membrane glycoprotein 2-like luminal" evidence="17">
        <location>
            <begin position="25"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Lysosome-associated membrane glycoprotein 2-like luminal" evidence="17">
        <location>
            <begin position="219"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="187"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="151"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="233"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="409" mass="44261" checksum="A8BC1147338557C5" modified="2019-05-08" version="1">MARVGGGRGLFLAARLLAFLQMSSSAFEVNDQSGKVCIIANLSASFSVEYHTKSGQQLTAHLALAQNAEVLANKSSCGKENVSAPILAIGFGAGHLLQMSFTKTSKLYSVDELTFIYNLSDTTVFPNSTGGEKNVSQKTNIQADLDTRYRCFSTNRINMSVVTITLSNVTLQAYLVNHTLSGNETVCAADKTPTTPVATTPTSQTPTTSQAPTSPPQNPMTGKYNVTGTNGTCVLAHMGLQLNVTYLRKDGKTGLDVLNFVPVNTSFSGSCDHSSAFLSLTFGRTRLVFQFLLNASTEKFFLQGVNVTTALPSEAKETKIDVFNHSMSELRAKLGNSYKCNAEEKLWVTDHVFVNLFDVQVQVFKIDENKFGAGKLHTSTLSSVVKGFNKIGKTCKFKHIGVFFSESSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H436</accession>
    <name>A0A452H436_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Lysosomal proton-coupled steroid conjugate and bile acid symporter SLC46A3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Solute carrier family 46 member 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>25-hydroxyvitamin D3 sulfate(out) + n H(+)(out) = 25-hydroxyvitamin D3 sulfate(in) + n H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:75491"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:194336"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>cholate(out) + n H(+)(out) = cholate(in) + n H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:75499"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29747"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dehydroepiandrosterone 3-sulfate(out) + n H(+)(out) = dehydroepiandrosterone 3-sulfate(in) + n H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:75487"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57905"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>estrone 3-sulfate(out) + n H(+)(out) = estrone 3-sulfate(in) + n H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:75483"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:60050"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>glycocholate(out) + n H(+)(out) = glycocholate(in) + n H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:75503"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29746"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>taurocholate(out) + n H(+)(out) = taurocholate(in) + n H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:75507"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36257"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the major facilitator superfamily. SLC46A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H436"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009381"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010782.1">
        <property type="protein sequence ID" value="ENSGAGP00000009381.1"/>
        <property type="gene ID" value="ENSGAGG00000007426.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034486">
        <property type="term" value="P:vacuolar transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF9">
        <property type="entry name" value="LYSOSOMAL PROTON-COUPLED STEROID CONJUGATE AND BILE ACID SYMPORTER SLC46A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="3 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="415"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="464" mass="51363" checksum="F8D4ED035BAC34EB" modified="2019-05-08" version="1">MRKVLLVEPVIIIYTFAAFLTVPLIQQYIYRRLWEEGSNSSLIENNNTTYCELNKSNPAYIKQKEVQEKASLFLMQLDLTGAVPSILIAIVLVADGDRHGRKKSLVLPSVGAFIANVFLGALSYFSLSLSILFAVAFFGSLFGSLATFLGGAFAFVADLCNEEKQKTIRIALVDMIFGLVSGLAGLTSGYFIRGLGFPWTFVVASLLHFINIFYITFCLEDTVRVPEFQQHASVSCTEGLKETFSGVYMLFKTSTCKKRTLIIVLLFTFMTYFFTWMGGSSLFTLYELDEPLCWNELYIGYGAAAFTSVSLTSFLGVYVFSRCLRDIYIVFIGIFSCIGGMVMAAFAKTTLLMFLVRVPSLLCFMPIPVLRSMLSKVVLASEQGAVFACIACLEVVTSAISVAVFNSLYAATVAWFPGFSFLLSAGLCIIPLGFMCWLMCTNWHEEDLASPAYEEVSSEESIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H644</accession>
    <name>A0A452H644_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TSC22 domain family protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TSC-22/Dip/Bun family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000010159"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011657.1">
        <property type="protein sequence ID" value="ENSGAGP00000010159.1"/>
        <property type="gene ID" value="ENSGAGG00000007965.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21938">
        <property type="entry name" value="ZIP_TSC22D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.490:FF:000002">
        <property type="entry name" value="TSC22 domain family, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000580">
        <property type="entry name" value="TSC22/Bun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047862">
        <property type="entry name" value="TSC22/BUN_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46745">
        <property type="entry name" value="TSC22 DOMAIN FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46745:SF1">
        <property type="entry name" value="TSC22 DOMAIN FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01166">
        <property type="entry name" value="TSC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58026">
        <property type="entry name" value="Delta-sleep-inducing peptide immunoreactive peptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01289">
        <property type="entry name" value="TSC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="787"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="881"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1014"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="972"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="791"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="898"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1020"/>
            <end position="1048"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1048" mass="108048" checksum="87FAF655AE6E5F8D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6F4</accession>
    <name>A0A452H6F4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. Required for efficient export of polyadenylated RNA. Plays a key structural role in the oligomerization of the THO-DDX39B complex. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THOC7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H6F4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010257"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011780.1">
        <property type="protein sequence ID" value="ENSGAGP00000010257.1"/>
        <property type="gene ID" value="ENSGAGG00000008038.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008501">
        <property type="entry name" value="THOC7/Mft1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405">
        <property type="entry name" value="MAINTENANCE OF KILLER 16 MAK16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405:SF5">
        <property type="entry name" value="THO COMPLEX SUBUNIT 7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05615">
        <property type="entry name" value="THOC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="204" mass="23866" checksum="BD0A71564DE519E8" modified="2019-05-08" version="1">MGAVTDDEVIRKRLLIDGDGAGDDRRINLLVKSFIKWCNSGSQEEGYSQYQRMLSTLSQCEFSMGKTLLVYDMNLREMENYEKIYKDIENSIAAAHEKIAECKKQILQAKRIRKNRQEYDALAKVIQHHPDRHETLKQLEALGKELQHLSHIKENVEDKLELRRKQFHVLLSTIHELQQTLENDEKLSEAEESQETHMEAETKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6U7</accession>
    <name>A0A452H6U7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the S-100 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H6U7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010259"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011783.1">
        <property type="protein sequence ID" value="ENSGAGP00000010259.1"/>
        <property type="gene ID" value="ENSGAGG00000008041.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00213">
        <property type="entry name" value="S-100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034325">
        <property type="entry name" value="S-100_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013787">
        <property type="entry name" value="S100_Ca-bd_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639:SF134">
        <property type="entry name" value="PROTEIN S100-A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639">
        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01023">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01394">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="147" mass="16145" checksum="3D89DA9C25CF5908" modified="2019-05-08" version="1">MCGCTSSTQNSKLYSVNQLGAASTQKTTSATANMPKRTPISKQLASVKALGKGSDLEKAFATVALVYNNSADPEGKLSKAETKTLLQTQFMNFIQGQESKPKYQEIISALDEDKDNKIDFEDFMILLLSLALMSDLLQEIRNLRTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7M9</accession>
    <name>A0A452H7M9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="14">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 14">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A452H7M9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010756"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012328.1">
        <property type="protein sequence ID" value="ENSGAGP00000010756.1"/>
        <property type="gene ID" value="ENSGAGG00000008376.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000022">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF84">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE VD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="315"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="15">
        <location>
            <begin position="55"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="11">
        <location>
            <position position="431"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="541" mass="61410" checksum="C666E5D32444AA45" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7Z6</accession>
    <name>A0A452H7Z6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H7Z6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010681"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012250.1">
        <property type="protein sequence ID" value="ENSGAGP00000010681.1"/>
        <property type="gene ID" value="ENSGAGG00000008335.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF20">
        <property type="entry name" value="ALPHA-2-ANTIPLASMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019276066" description="Serpin domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="4">
        <location>
            <begin position="29"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="421" mass="47467" checksum="6AD5E865BBD110F5" modified="2019-05-08" version="1">AMLLWGLLLCLSTLHDSPGVRHRHKALPSPVLQDISSVSTLFTPVSQGQWQEQGRQCQAGLEQAANQTEKRLLEALHLESVPCLHHLLSSIRRQLTGSMLSVASRVYLQKGFEVKEKFLENSERFYGAKPMILSGNNENDLIAINKWIKEATEGQISTFLDQLPASTVMLLLNAVYFQGFWRHKFDPSQTGPDVFHLDDQFIVPVEMMKAQKYSLSWFTLDPLEVQVARFPFKGNMSFVAIVPNQFKWNVSQVLVNLSHDKLHSRFPKEKPTMVKMPKLHVDYQLELNQVLSQLGLGELFSTPDLQKITDEPLFVSSIQHQSTLELAEDGVEASAATSVMMSRSLSTFSLNQPFLFIIFEETTGIPLFVGSIQNPNPSAAQQKKEPQDSPTLKNHITVNPANTSLFTRAERLTPRHHTDIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8S5</accession>
    <name>A0A452H8S5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H8S5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011144"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012764.1">
        <property type="protein sequence ID" value="ENSGAGP00000011144.1"/>
        <property type="gene ID" value="ENSGAGG00000008624.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050772">
        <property type="term" value="P:positive regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051505">
        <property type="entry name" value="C-type_lectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789:SF1">
        <property type="entry name" value="CHONDROLECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789">
        <property type="entry name" value="CHONDROLECTIN VARIANT CHODLFDELTAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="C-type lectin" evidence="7">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="208" mass="23592" checksum="5DFE547EE2E4F1F1" modified="2019-05-08" version="1">MLLSLASETEQKLIENMLQNLTKSGSGISDGDFWIGLWRSGEGQATSSACPELYQWTDGTISSFRNWYTDEPSCGSEACVVMYHQPTANPGLGGPYLYQWNDDRCNMKHNFICKYSLDDVLEKELGDRTDTDYEVFPSVTAGKPYDENKSEDNSHVVVTETVKEEQKPAQTSPHYGFQRTPGRTVPWRYNNLMTENKAKIIFYSQALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H961</accession>
    <name>A0A452H961_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H961"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011291"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012931.1">
        <property type="protein sequence ID" value="ENSGAGP00000011291.1"/>
        <property type="gene ID" value="ENSGAGG00000008733.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15911">
        <property type="entry name" value="7tmA_OR11A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="137"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="194"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="38"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="344" mass="39208" checksum="833BD891C7572CEB" modified="2025-10-08" version="2">MSLQEHMEFILLGFGNIPKLQVFLFLLFLAIYFVTAAGNILIIVLVVADQQLHTPMYFFLANLSCLETCYTSTILPRMLASFLTGNRTISVNGCLTQYYLFGFFGATECSLLAAMSYDRYFAICKPLHYATLMNGSLCLQLVTASWICGFLTIPIIVFLVLQFTFCGPNEIDNFFCEFLQIINLYCNATYQMELVVTILVAVFTLPPFALTLASYVCIISSILRIPSTIGRQKAFSTCSSHLIMVTIFYGTLIIVYLLPKTNTLRDLNKVFSVCYTIVTPMANPFIYSLRNQEVKEALRKIVRKCLDFMRIQKLQSLQAKWCITQILCGPYFLLHCWRYALITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H993</accession>
    <name>A0A452H993_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Leucine-rich repeat transmembrane protein FLRT3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Cell projection</location>
            <location evidence="21">Growth cone membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H993"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011142"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012758.1">
        <property type="protein sequence ID" value="ENSGAGP00000011142.1"/>
        <property type="gene ID" value="ENSGAGG00000008619.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000043">
        <property type="entry name" value="Leucine-rich repeat transmembrane protein FLRT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF12">
        <property type="entry name" value="LEUCINE-RICH REPEAT TRANSMEMBRANE PROTEIN FLRT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="15" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="11 25" id="KW-0732">Signal</keyword>
    <keyword evidence="10 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019470131" description="Leucine-rich repeat transmembrane protein FLRT3" evidence="25">
        <location>
            <begin position="29"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="527"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="26">
        <location>
            <begin position="406"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="626"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00037815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00039161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="649" mass="73552" checksum="382FA6DE13C65F01" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HD33</accession>
    <name>A0A452HD33_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HD33"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012704"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014564.1">
        <property type="protein sequence ID" value="ENSGAGP00000012704.1"/>
        <property type="gene ID" value="ENSGAGG00000009749.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15222">
        <property type="entry name" value="7tmA_OR51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000002">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF87">
        <property type="entry name" value="OLFACTORY RECEPTOR 51F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="47"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="320" mass="36055" checksum="4D58D03D25DF8F41" modified="2019-05-08" version="1">MTHTMPLANSSFYQPSTFLLTGFPGLEANHHWISIPFCVFYLIGLSGNCLILIIIKKTQSLHKPMYYFLSMLAMTDLGLALCTLPTTLGIFWFKIRKIEFNACLTQMYFIHILSVMESSVLLAMAFDRFIAISNPLRYSSILTESTIIKTGLAIISRAVISLLPIPFLLKRLTYCGDNVLSHSFCFHPDIMKLACADIKVNILYGLIVILSTVGVDFVFIVLSYVLILKTVVSLTTKEKCLKALNTCVSHICAVLIFFIPMIGLSVLHRCGNNVPPMLNIVVAYIYLIVPPVLNPIIYSVKSKLIRRAMLRTLWWKNESM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDD6</accession>
    <name>A0A452HDD6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H(+)-transporting two-sector ATPase</fullName>
            <ecNumber evidence="2">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452HDD6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012802"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014676.1">
        <property type="protein sequence ID" value="ENSGAGP00000012802.1"/>
        <property type="gene ID" value="ENSGAGG00000009830.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042592">
        <property type="term" value="P:homeostatic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000008">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000052">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005725">
        <property type="entry name" value="ATPase_V1-cplx_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003220">
        <property type="entry name" value="PRK04192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01042">
        <property type="entry name" value="V-ATPase_V1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF8">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="12">
        <location>
            <begin position="22"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="ATPsynthase alpha/beta subunit barrel-sandwich" evidence="13">
        <location>
            <begin position="124"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="11">
        <location>
            <begin position="230"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="14">
        <location>
            <begin position="464"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="604" mass="66607" checksum="6BE668B64AD29113" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDE9</accession>
    <name>A0A452HDE9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thyrotropin subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Thyroid-stimulating hormone subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Thyrotropin beta chain</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycoprotein hormones subunit beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HDE9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012855"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014734.1">
        <property type="protein sequence ID" value="ENSGAGP00000012855.1"/>
        <property type="gene ID" value="ENSGAGG00000009866.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00069">
        <property type="entry name" value="GHB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000007">
        <property type="entry name" value="Luteinizing hormone beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001545">
        <property type="entry name" value="Gonadotropin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018245">
        <property type="entry name" value="Gonadotropin_bsu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11515">
        <property type="entry name" value="GLYCOPROTEIN HORMONE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11515:SF5">
        <property type="entry name" value="THYROTROPIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00068">
        <property type="entry name" value="GHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00261">
        <property type="entry name" value="GLYCO_HORMONE_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00689">
        <property type="entry name" value="GLYCO_HORMONE_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0372">Hormone</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019542906" description="Thyrotropin subunit beta" evidence="12">
        <location>
            <begin position="19"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoprotein hormone subunit beta" evidence="13">
        <location>
            <begin position="20"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="134" mass="15138" checksum="6E9E3EB5F133DD51" modified="2019-05-08" version="1">MSPIFLMSLLFGLTFGQAMSFCAPTEYIIHVEKRECAYCLAINTTICAGFCMTRDSNGKKLLLKSALSQDVCTYKDMVYRTVVLPGCPRHTVPYYSYPVAMNCKCGKCNTDYSDCIHETVRTDYCTKPQKSYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEU0</accession>
    <name>A0A452HEU0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HEU0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013339"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015267.1">
        <property type="protein sequence ID" value="ENSGAGP00000013339.1"/>
        <property type="gene ID" value="ENSGAGG00000010208.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029234">
        <property type="entry name" value="CIMIP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31702">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31702:SF2">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15400">
        <property type="entry name" value="TEX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="289" mass="32126" checksum="BE25EA3A67E73353" modified="2019-05-08" version="1">MSSPQVKSQASPSAQHLHTRGYSKQSLRSKTPSAWCHLTREGEEARDVSGVKEPAVGQHQRGRTGSLLHLAKSTQKEADSVQAYSNGKEPAAGQHRPLGPRVPGSPRKTTKTSHKEVREFRNGLSAKESLASIGQDVKAEGKLFQEKKNSIIPINIKHKFGSSIVDELVTEEQARRALCEAGLIEGQKRLSNRSSKTPENPMATTPFADYYELGYNLRSNIFQGGPIESKSLMKDSYTPDVIQRAVRDPKHWHGRKTDDLGRWHQKNALNLNLQKALEQKYGEKTKGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEY9</accession>
    <name>A0A452HEY9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HEY9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013389"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015322.1">
        <property type="protein sequence ID" value="ENSGAGP00000013389.1"/>
        <property type="gene ID" value="ENSGAGG00000010242.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="71" mass="7745" checksum="222DF0A993F8EB87" modified="2019-05-08" version="1">MSVCAGVCTSTPCFLSEKAEKLARGAALKWASGVFYRPDKLEGLGHYRSREAQRNSSIQSRLKVGARCRGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFG6</accession>
    <name>A0A452HFG6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TASOR family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000013546"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015507.1">
        <property type="protein sequence ID" value="ENSGAGP00000013546.1"/>
        <property type="gene ID" value="ENSGAGG00000010349.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097355">
        <property type="term" value="P:protein localization to heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22569">
        <property type="entry name" value="TASOR_PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056242">
        <property type="entry name" value="PIN_TASOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046432">
        <property type="entry name" value="TASOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056243">
        <property type="entry name" value="TASOR_ab_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022188">
        <property type="entry name" value="TASOR_DUF3715"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16207:SF1">
        <property type="entry name" value="PROTEIN TASOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16207">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12509">
        <property type="entry name" value="DUF3715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24630">
        <property type="entry name" value="PIN_TASOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23314">
        <property type="entry name" value="TASOR_alpha-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TASOR pseudo-PARP" evidence="3">
        <location>
            <begin position="181"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="TASOR alpha/beta" evidence="4">
        <location>
            <begin position="1271"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="domain" description="TASOR PIN" evidence="5">
        <location>
            <begin position="1369"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="649"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="716"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="953"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1214"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1537"/>
            <end position="1556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1565"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1693"/>
            <end position="1718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="67"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="737"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="751"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="959"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1214"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1538"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1579"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1592"/>
            <end position="1601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1718" mass="192779" checksum="D100957757E98177" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHB8</accession>
    <name>A0A452HHB8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5-taurinomethyluridine-[tRNA] synthase subunit MTO1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mitochondrial tRNA translation optimization 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Protein MTO1 homolog, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the GTPBP3-MTO1 complex that catalyzes the 5-taurinomethyluridine (taum(5)U) modification at the 34th wobble position (U34) of mitochondrial tRNAs (mt-tRNAs), which plays a role in mt-tRNA decoding and mitochondrial translation. Taum(5)U formation on mammalian mt-tRNA requires the presence of both GTPBP3-mediated GTPase activity and MTO1 catalytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>5,10-methylenetetrahydrofolate + uridine(34) in tRNA + taurine + GTP + A + H2O = 5-taurinomethyluridine(34) in tRNA + 7,8-dihydrofolate + GDP + AH2 + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:83279"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="Rhea" id="RHEA-COMP:11732"/>
            <dbReference type="ChEBI" id="CHEBI:12071"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:87172"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:83280"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer; forms a dimer in the presence of potassium. Interacts with GTPBP3; forms the GTPBP3-MTO1 complex composed of homodimers of GTPBP3 and MTO1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HHB8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014263"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016322.1">
        <property type="protein sequence ID" value="ENSGAGP00000014263.1"/>
        <property type="gene ID" value="ENSGAGG00000010854.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070899">
        <property type="term" value="P:mitochondrial tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000082">
        <property type="entry name" value="protein MTO1 homolog, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.570:FF:000001">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000002">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="entry name" value="GidA associated domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049312">
        <property type="entry name" value="GIDA_C_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047001">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00136">
        <property type="entry name" value="mnmG_gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="GLUCOSE INHIBITED DIVISION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PROTEIN MTO1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21680">
        <property type="entry name" value="GIDA_C_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="SAM_GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01228">
        <property type="entry name" value="GIDA_assoc_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01281">
        <property type="entry name" value="GIDA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA uridine 5-carboxymethylaminomethyl modification enzyme C-terminal subdomain" evidence="12">
        <location>
            <begin position="512"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="601"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="607"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="625" mass="69793" checksum="EA7963B1656EDE22" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHN7</accession>
    <name>A0A452HHN7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Methylthioribulose-1-phosphate dehydratase</fullName>
            <shortName evidence="9">MTRu-1-P dehydratase</shortName>
            <ecNumber evidence="9">4.2.1.109</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">APAF1-interacting protein homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">APIP</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway. May play a role in apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-(methylsulfanyl)-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15549"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="4.2.1.109"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 2/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldolase class II family. Adducin subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the aldolase class II family. MtnB subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.109" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452HHN7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014394"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016465.1">
        <property type="protein sequence ID" value="ENSGAGP00000014394.1"/>
        <property type="gene ID" value="ENSGAGG00000010941.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00875"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046570">
        <property type="term" value="F:methylthioribulose 1-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000003">
        <property type="entry name" value="Methylthioribulose-1-phosphate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03116">
        <property type="entry name" value="Salvage_MtnB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017714">
        <property type="entry name" value="MethylthioRu-1-P_deHdtase_MtnB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027514">
        <property type="entry name" value="Salvage_MtnB_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03328">
        <property type="entry name" value="salvage_mtnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10640">
        <property type="entry name" value="METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10640:SF7">
        <property type="entry name" value="METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="10">
        <location>
            <begin position="30"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="246" mass="27331" checksum="07050F5F3574294A" modified="2019-05-08" version="1">MAAAGGGLCCPRGGSCVAGDVSQDKLHPRNLIPELCKQFYHLGWVTGTGGGISLKHGDEIYIAPSGVQKERIQPEDMFVCDMEERDISGPPPHKKLKKSQCTPLFMNAYTMRGAGAVIHTHSKAAVMATLLYPGSEFRITHQEMIKGIKKCTSGGYYRYDDKLVVPIVENTPEEKDLKERMAHAMNEYPDSCAVLVRRHGVYVWGETWEKAKTMCECYDYLFDIAVQMKLHGLDPAKHPAEENGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHP2</accession>
    <name>A0A452HHP2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HHP2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014399"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016470.1">
        <property type="protein sequence ID" value="ENSGAGP00000014399.1"/>
        <property type="gene ID" value="ENSGAGG00000010946.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13265">
        <property type="entry name" value="PH_evt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000073">
        <property type="entry name" value="Pleckstrin homology domain-containing family B member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039680">
        <property type="entry name" value="PLEKHB1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14309">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14309:SF8">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY B MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="2"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="222" mass="25091" checksum="F8CBF80C85CFFAE1" modified="2019-05-08" version="1">MAFVKSGWLLRQSTILRRWKKNWFDLWSDGHLLFYDDQQRHDIEDKVHMRVHCINIRVGNECRDFQPPEGKPRDCLLQIVCRDGKTINLCAESADDCLAWKIALQDARTNAGYVGSEAMYDETAISSAPPPYTAYATPSPEVYGYEQYNGAYPPPGHQVIYASNGQAYAVPYQYPYQGPYGQPPANHVIIRERYRDNDGDLALGMLAGAATGMALGSLFWVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIP7</accession>
    <name>A0A452HIP7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">N-acetyltaurine hydrolase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Phosphotriesterase-related protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N-acetyl-L-isoleucine + H2O = L-isoleucine + acetate</text>
            <dbReference type="Rhea" id="RHEA:81119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:133735"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:81120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N-acetyl-L-leucine + H2O = L-leucine + acetate</text>
            <dbReference type="Rhea" id="RHEA:81115"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:58270"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:81116"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-acetyl-L-methionine + H2O = L-methionine + acetate</text>
            <dbReference type="Rhea" id="RHEA:67440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:71670"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67441"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N-acetyl-L-valine + H2O = L-valine + acetate</text>
            <dbReference type="Rhea" id="RHEA:81123"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:133716"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:81124"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-acetyltaurine + H2O = taurine + acetate</text>
            <dbReference type="Rhea" id="RHEA:81107"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:133737"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:81108"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N-propanoyltaurine + H2O = propanoate + taurine</text>
            <dbReference type="Rhea" id="RHEA:81111"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17272"/>
            <dbReference type="ChEBI" id="CHEBI:231795"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:81112"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="11">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the metallo-dependent hydrolases superfamily. Phosphotriesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HIP7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014764"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016872.1">
        <property type="protein sequence ID" value="ENSGAGP00000014764.1"/>
        <property type="gene ID" value="ENSGAGG00000011187.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00530">
        <property type="entry name" value="PTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001559">
        <property type="entry name" value="Phosphotriesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10819">
        <property type="entry name" value="PHOSPHOTRIESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10819:SF3">
        <property type="entry name" value="PHOSPHOTRIESTERASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02126">
        <property type="entry name" value="PTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016839">
        <property type="entry name" value="PhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51347">
        <property type="entry name" value="PHOSPHOTRIESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601559-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="349" mass="39048" checksum="9F7443B839843C21" modified="2019-05-08" version="1">MPSLSGKVQTVLGLVEPSQLGCTMTHEHLTMNYSCCYVPPSSGQEALSDGPIEMKNLFWIKQNPYSHKENLLLCQETDAVKEELLHYKAAGGGTIVENTTMGISRDVKTLKKLAEETEVHIIAGAGFYVHATHSSKTQAMSVEQLTDIIVNEILSGADGTNIKCGVVGEIGCSWPLTESENKVLQATAHAQSQLGCPIIIHPGRNSDAPFQIIRILQETGADISKTVMSHLDRTIFDEKKLLEFAKLGCYLEYDLFGTELLHYHFHPEIDMPSDNERIARIRMLIDEGYEDRIVIAHDMHTKNRLMKYGGHGYSHILQNIVPKMLIRGIPQNIIDKILIENPKQWLTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJH5</accession>
    <name>A0A452HJH5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Hemojuvelin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Hemojuvelin BMP coreceptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">RGM domain family member C</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with BMP2 and BMP4. Interacts with BMP6. Interacts with BMPR1B. Interacts with TMPRSS6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the repulsive guidance molecule (RGM) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJH5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015065"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017211.1">
        <property type="protein sequence ID" value="ENSGAGP00000015065.1"/>
        <property type="gene ID" value="ENSGAGG00000011381.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1000.10:FF:000003">
        <property type="entry name" value="hemojuvelin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040287">
        <property type="entry name" value="RGM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009496">
        <property type="entry name" value="RGM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010536">
        <property type="entry name" value="RGM_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428:SF3">
        <property type="entry name" value="HEMOJUVELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428">
        <property type="entry name" value="RGM DOMAIN FAMILY MEMBER DRAG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06534">
        <property type="entry name" value="RGM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06535">
        <property type="entry name" value="RGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="466"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Repulsive guidance molecule N-terminal" evidence="20">
        <location>
            <begin position="118"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Repulsive guidance molecule C-terminal" evidence="19">
        <location>
            <begin position="226"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF06534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF06535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="487" mass="53238" checksum="F6FD99C965F228C4" modified="2019-05-08" version="1">MVECMESAWPPSLPGTASSPDPVSWNVPSGKHAVGSADPGGWHRSPGWSLLAAAWLKDLAGELVKEKKKRGCLIPIGSGWIGMGKSACSTSPQQLESTCFILRALFLLLFCRHACCQCKILRCNADYVAATLNLRGSNKDAAYCNALRSYSHCTRKTARTCRGDLVYHSAVHGIEDLMIQHNCSKEGPTSPPRPRPPAPNHQGFESLDICNYEKSFLYKHGQPPTYQHCAAFGDPHVRTFQDEFHTCRVEGSWPLLDNNYLFVQATSYLVAKSSNATATGKLTIIFKNMKECIDQKVYQAEIDNLPAAFEDGSVNGGERPGGSSLTIQEHAPGQHVEIRAAYIGTIIAVRQAGRQLSFSIRAAEEVARSFTEEQDLQLCVGGCPPSQRISRSECRHTGIATETARLLCKEKLPVEDVYFQSCVFDVVTSGDANFTLAAHGALEDAKMFHPDAEKLHIFQTGAGCCLISLPFLLVIVTSNLLAIQLCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HK36</accession>
    <name>A0A452HK36_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HK36"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015284"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017461.1">
        <property type="protein sequence ID" value="ENSGAGP00000015284.1"/>
        <property type="gene ID" value="ENSGAGG00000011533.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026646">
        <property type="entry name" value="GPRIN2-like/GPRIN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15718:SF6">
        <property type="entry name" value="G PROTEIN-REGULATED INDUCER OF NEURITE OUTGROWTH 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15718">
        <property type="entry name" value="G PROTEIN-REGULATED INDUCER OF NEURITE OUTGROWTH C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="490" mass="52477" checksum="806ADA8B1C1FD999" modified="2019-05-08" version="1">MGTVPDPLRSAKLLLVATSEERDCLGDPQSTKHQHKLASLERSSNGISYTQAESAGVCLFDLNCTVVSDIQRDERLCGDDTSQPDMFLPGSFSQAEEVPPACTESASDSEQKVSSDSTVQTPIIARTCAAGHATDMMPATHNSNQLIQSDLPRIKSLAQVDDCAVKVCRMNDPAELQELNYPSPDNTIKGSESCHHSSQANTLQRIDMENEKVVEINDPGFSLAATPVRDSEAIPDLGIRLQVYSEAEIRAANDDVKSCPTFRTALAQSNEASPPSSFEGTLPVCKAETEKTGPVLSQLSRFREASTMTVQPESTSLTQEAVNRTWRDAEVQAVASMETKSASTSPSILAAFLKGNLPSEANEQLHIIYQSGDGQNQSELADDFASLQEPALCLGIAPEMCALTVVMDTVKAQPAKLQKHQGDPPDTICPVLSGSTTPACLCPQEAGNTKGIPADGAETKVAFFTRDSTDIPQLPSDAAVLLRAKPVYHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNG7</accession>
    <name>A0A452HNG7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HNG7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016508"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018840.1">
        <property type="protein sequence ID" value="ENSGAGP00000016508.1"/>
        <property type="gene ID" value="ENSGAGG00000012350.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029638">
        <property type="entry name" value="FAM78"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31655">
        <property type="entry name" value="PROTEIN FAM78A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31655:SF3">
        <property type="entry name" value="PROTEIN FAM78A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="283" mass="31700" checksum="83FB08B8A2C0B5B6" modified="2019-05-08" version="1">MPNLPQDCWSVLGIALVLYAMGCIQSISCKSKVFRESISVIEVKASIDPVPTSIDESSSVVLRYRTPHFRASAQVLVPPISKKETWIVGWIQACSHMEFYNHYGEQGMSSWELPDLQDGKIQAISDSDGVNYPWYGNTTETCMIVGPTRKESKFSISMNDNFYPSVTWAVPVSESNVAKLTSIHRDQSFTTWLVATNTATSEMVTLQTIKWRMRLGIDVNPSRPLGQRATLRQPFAQEQPQVLSKNEPIPPSALVKPNANDAQVLMWRPKEGPPLVVIPSKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNK7</accession>
    <name>A0A452HNK7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HNK7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016550"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018886.1">
        <property type="protein sequence ID" value="ENSGAGP00000016550.1"/>
        <property type="gene ID" value="ENSGAGG00000012375.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF17">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="469" mass="51835" checksum="A0CA81E017E2FEFE" modified="2019-05-08" version="1">MSCERLTLDCLVHVFSYLEAPDLLRAARVNQAWNEAAGTTSLWRRLCLDRWTFCNISNLTPGMQTWKQYYLHRSKLEHEMASGRPSADYKCCPMRGHKGKIVGMAYLSDNEHVFGGGKLRSVVCTASTDGTIRAWQVQEGTQIWSSPSQEVPFIKVITVPEYKLAISTDARGTIKVWHGESGEELAAFSTSSSSSTLVTYSVNNKPFLTAATAGGVIYTLEVPNLNQVSCITAFQNSSIDLLLCSPDEQWLVAGSTENADTSSKIFYTDCLINPADESPASGSLPVNNCDVGCWLPKAPARIAVMHQDHMSVQRNITVFDLAVKKSKYKMEVLAQQVASFTLPGRTWTTPILMKGHGTETILLGCGSNLELYSIFGTQLEAFQHHNNTITSIWVDAFRVITSSLDLSLRVYTWKKVNKISSLTSRYYLLGGTHRWSRGFTDVACDNISIVGVVAESDGTSILRAYSFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HP57</accession>
    <name>A0A452HP57_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HP57"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016785"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019159.1">
        <property type="protein sequence ID" value="ENSGAGP00000016785.1"/>
        <property type="gene ID" value="ENSGAGG00000012539.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035613">
        <property type="term" value="F:RNA stem-loop binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41694">
        <property type="entry name" value="ENDOGENOUS RETROVIRUS GROUP K MEMBER POL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41694:SF3">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNase H type-1" evidence="7">
        <location>
            <begin position="81"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="290"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="506" mass="56078" checksum="34A899EE39642713" modified="2019-05-08" version="1">MLLAASSARHWSQLPIPGTLSGPCVHLIPWLTSTPPPEFHGTTRTWQLLCFQVEVAWQAWTLTPSDNILAPPLHQPLCIEVKYDPWVVSDGGTSPSPRAGVLCSTCNHFNVQLLPPSCSAQECEVQGVLLAAQHVANAHSANKQVVLGIDSQFCLDILIEQASPSAFVKLWDQIFALIRKFSHPWFVTHCPSHRPDSNPLHSSLDQRMRELSQVHMANPAVQADPLLRWLHEEWGHLPPTKLYGLLSSFSNQKPDVSRSQCELIVQSCLQCVQVKTMNRPRYERSAYAAEHFAPGSTWQVDLIGPLPGARRFPKALVTVDLGSRQTFCVPLNKTNAASVAVALKQTAAAWGTPTEVQADGGPPFTSKSIEQLIFGWGASLHIHTKYHPQSNGVVERKIAVLKTMLRTANPNPDFKNWSSFLPKVLIALNKSYSRWPHISSTPKPPWSPMFNTGQLVWLTEPQASGHRKPIKTTILRAGKYPNTYLVATSTPGNKLVHHNWLSKCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRY9</accession>
    <name>A0A452HRY9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HRY9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017839"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020355.1">
        <property type="protein sequence ID" value="ENSGAGP00000017839.1"/>
        <property type="gene ID" value="ENSGAGG00000013241.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031885">
        <property type="entry name" value="DUF4764"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039946">
        <property type="entry name" value="ZN839"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16116">
        <property type="entry name" value="ZINC FINGER PROTEIN 839"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16116:SF5">
        <property type="entry name" value="ZINC FINGER PROTEIN 839"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15961">
        <property type="entry name" value="DUF4764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="503"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="608"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="625"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="648"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1187" mass="127982" checksum="82F2CF77A10B2487" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUA3</accession>
    <name>A0A452HUA3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HUA3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018627"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021235.1">
        <property type="protein sequence ID" value="ENSGAGP00000018627.1"/>
        <property type="gene ID" value="ENSGAGG00000013783.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000506">
        <property type="entry name" value="Zinc finger protein 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000269">
        <property type="entry name" value="Zinc finger protein 287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000087">
        <property type="entry name" value="Zinc finger protein 354B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001767">
        <property type="entry name" value="Zinc finger protein 629"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="157"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="348" mass="39623" checksum="EDB0B9D5615DBDA6" modified="2019-05-08" version="1">MSCSAEQGEASGAGRQQETEVISPDETDIKVVTEHNDAQAEVRPYSCLDCGKSFSQSSHLVQHQRTHTGQKPYKCTECGKCFVLSYNLIDHQRIHTGERPYKCGQCGKGFIRSSHLFQHQRIHTGERPYQCTKCGKSFKCSYTLVKHYHTHVGEPPCFCSECGKSFSLGSFAKHVRTHTGEKPYSCVECGESFISSSNLIQHRRIHTGEKPYSCGHCGKSFSQSANLIKHRRIHTGEKPYSCSECSKTFSQSSSLMRHRRIHRRDQPYECTECGKRFRCNSSLMNHQRIHTGERPYKCPECGKGFINRSRLTNHRRTHTGERPYICTDCGKGFGDSSALMKHQRMHTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVJ0</accession>
    <name>A0A452HVJ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Triokinase/FMN cyclase</fullName>
            <ecNumber evidence="5">2.7.1.28</ecNumber>
            <ecNumber evidence="4">2.7.1.29</ecNumber>
            <ecNumber evidence="6">4.6.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>D-glyceraldehyde + ATP = D-glyceraldehyde 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13941"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.28"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>FAD = riboflavin cyclic-4',5'-phosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13729"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:76202"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.6.1.15"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>dihydroxyacetone + ATP = dihydroxyacetone phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15773"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="23">Homodimer. Interacts with IFIH1 (via the CARD domains), the interaction is inhibited by viral infection.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.28" evidence="5"/>
    <dbReference type="EC" id="2.7.1.29" evidence="4"/>
    <dbReference type="EC" id="4.6.1.15" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452HVJ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019099"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021767.1">
        <property type="protein sequence ID" value="ENSGAGP00000019099.1"/>
        <property type="gene ID" value="ENSGAGG00000014126.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034012">
        <property type="term" value="F:FAD-AMP lyase (cyclizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004371">
        <property type="term" value="F:glycerone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050354">
        <property type="term" value="F:triokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.340:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10440:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase, DhaK subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1180.20:FF:000003">
        <property type="entry name" value="triokinase/FMN cyclase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10440">
        <property type="entry name" value="Dihydroxyacetone kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.20">
        <property type="entry name" value="Dihydroxyacetone kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012734">
        <property type="entry name" value="DhaK_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004006">
        <property type="entry name" value="DhaK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004007">
        <property type="entry name" value="DhaL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036117">
        <property type="entry name" value="DhaL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050861">
        <property type="entry name" value="Dihydroxyacetone_Kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02361">
        <property type="entry name" value="dak_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011049">
        <property type="entry name" value="PRK14479.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28629">
        <property type="entry name" value="TRIOKINASE/FMN CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28629:SF4">
        <property type="entry name" value="TRIOKINASE_FMN CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02733">
        <property type="entry name" value="Dak1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02734">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01120">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82549">
        <property type="entry name" value="DAK1/DegV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101473">
        <property type="entry name" value="DhaL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51481">
        <property type="entry name" value="DHAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51480">
        <property type="entry name" value="DHAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="20" id="KW-0170">Cobalt</keyword>
    <keyword evidence="14" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0456">Lyase</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DhaK" evidence="30">
        <location>
            <begin position="10"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="DhaL" evidence="29">
        <location>
            <begin position="372"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-hemiaminal-histidine intermediate" evidence="27">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00046681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR612734-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR612734-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="578" mass="58939" checksum="21CCA607F5D5CE03" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HW28</accession>
    <name>A0A452HW28_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4604 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HW28"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019322"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022006.1">
        <property type="protein sequence ID" value="ENSGAGP00000019322.1"/>
        <property type="gene ID" value="ENSGAGG00000014261.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027911">
        <property type="entry name" value="DUF4604"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040219">
        <property type="entry name" value="KIAA1143-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31195">
        <property type="entry name" value="GEO02494P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31195:SF2">
        <property type="entry name" value="GEO02494P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15377">
        <property type="entry name" value="DUF4604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4604" evidence="2">
        <location>
            <begin position="6"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="157" mass="17571" checksum="9C5C94AAD2C5277D" modified="2019-05-08" version="1">MSKKTQVSYVPPAEPAFLSRLKRQVGYREGPTVETKREQLPAPEEDCESGSDKEDEQPQVVVLKKGDLTPEEVMKIKQEIKDASKSDEEPEPDDGKIVFKKPSKRSSEEKFSGLTASSSKKKKESKKTKRDSAPPQNAAKQIKNSSLLSFDDEENDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HX85</accession>
    <name>A0A452HX85_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HX85"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019713"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022455.1">
        <property type="protein sequence ID" value="ENSGAGP00000019713.1"/>
        <property type="gene ID" value="ENSGAGG00000014533.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000022458.1">
        <property type="protein sequence ID" value="ENSGAGP00000019716.1"/>
        <property type="gene ID" value="ENSGAGG00000014533.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000022">
        <property type="entry name" value="methylglutaconyl-CoA hydratase, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF12">
        <property type="entry name" value="METHYLGLUTACONYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="321" mass="33799" checksum="038BFCE095E6F07B" modified="2019-05-08" version="1">MAASLGGLGLLQGRVRLAAAAAASSPLSRPGARGLVRGGAACSPTGRGYSSEAKGEEELRVRYLDEENQGIVVLGLNRSRAKNALNKNLIKMMSKAVDALKSDKKVRTVIFRSEVPGIFCAGADLQERAKMHSSEVSSFVSKARATINEMANLPVPTIAAIDGTALGGGLELALACDIRVAASSAKMGLVETKLAIIPGAGGTQRLPRTIGVSLAKELIFSARIVDGEEAKSIGLISHVVEQNESGDAAYRRALALAQEFLPQGPLAIRLAKLAINQGMEVDLVTGLAIEEACYAQTIPTKDRIEGLLAFKEKRPPRYKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYA4</accession>
    <name>A0A452HYA4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Potential calcium-dependent cell-adhesion protein.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with DAB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYA4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020142"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022956.1">
        <property type="protein sequence ID" value="ENSGAGP00000020142.1"/>
        <property type="gene ID" value="ENSGAGG00000014866.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000003">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000103">
        <property type="entry name" value="protocadherin-18 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000133">
        <property type="entry name" value="protocadherin-18 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF9">
        <property type="entry name" value="PROTOCADHERIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="706"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="33"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="145"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="254"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="376"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="473"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="588"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="775"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="874"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="959"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="784"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="874"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="960"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1139" mass="127057" checksum="B86264F57D52E176" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYF1</accession>
    <name>A0A452HYF1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HYF1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020201"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023016.1">
        <property type="protein sequence ID" value="ENSGAGP00000020201.1"/>
        <property type="gene ID" value="ENSGAGG00000014899.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000556">
        <property type="entry name" value="Immunoglobulin heavy variable 7-81 (non-functional)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019168529" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="18"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="119" mass="13070" checksum="A226B3B50DE54DD6" modified="2019-05-08" version="1">DSLAWHLLLVILGISVSSQIQLTQSGAEIKKPGESVRVTCKTSGYTFTSYSINWFRQVPGKALEWIGYIAPSTGGTGYAQAFQGRFTITRDTSISTAYLQLGSLRTEDTATYYCARDTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYT9</accession>
    <name>A0A452HYT9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HYT9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020406"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023249.1">
        <property type="protein sequence ID" value="ENSGAGP00000020406.1"/>
        <property type="gene ID" value="ENSGAGG00000015036.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15227">
        <property type="entry name" value="7tmA_OR14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="55"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="307" mass="34341" checksum="43310A743112E6B8" modified="2025-10-08" version="2">MLSPLVFPLQCTSTEEMSNQTIVTEFLLLGFSDMREEQILHFLVFLVIYLAALVGNLLIITVIAINQHLHTPMYFFLGNLSFLDLCYISVTVPKSMADSLTNNRLISFSGCVTQVFLVITFAGVELAFLTVMAYDRYIAICHPLHYKVTMNRGACAKLAVSSWISSVICSVLHTLLKISCSDTHANVIVMIALGSVLGVVCFVFIIVSYIHIFSTVMRISSEQGRYKAFSTCIPHLVVFCLFISTASFTYMRPRSMSSISLDLMAAVLYCVVPPLMNPIIYSLRNKEIKGSLWKMIEHLPEQNSEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZC9</accession>
    <name>A0A452HZC9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Proline-serine-threonine phosphatase-interacting protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to F-actin. May be involved in regulation of the actin cytoskeleton.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HZC9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020576"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023433.1">
        <property type="protein sequence ID" value="ENSGAGP00000020576.1"/>
        <property type="gene ID" value="ENSGAGG00000015146.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07672">
        <property type="entry name" value="F-BAR_PSTPIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000054">
        <property type="entry name" value="Proline-serine-threonine phosphatase interacting protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042694">
        <property type="entry name" value="PSTPIP2_F-BAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF9">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="10">
        <location>
            <begin position="4"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="335" mass="39186" checksum="B4856EE0D7A84D3F" modified="2019-05-08" version="1">MKETLFKGNFWSTDLTSTVGYDSIVQHLNDGRKNCKEFEDFLKERATIEEKYGKELINLTKKKPCGQTELNTLKRALEVFKQQVDGVGQSHIQLAQTLREEARKMEDFREKQKLHRKKIELVMDAIHKNRNLQYKKTVEAKRLYEQRCRDKDEAEQAVHRNANLVTQKQQEKLFVKLAQSKSALEDSDRQYQLNVGALEKIREEWQNEHTKACEFFETQESERINYFRNALWLHLNQLSQECVRSDERYEETRRSLEMCSIEKDIEFFVNFRKTGSVPPGPVVYENYYNTQRNATTMRNQAPAPGIGRRGPLPIPASGPGDPDYATVDGYSLIHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4A4</accession>
    <name>A0A452I4A4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Atos-like conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I4A4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022372"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025493.1">
        <property type="protein sequence ID" value="ENSGAGP00000022372.1"/>
        <property type="gene ID" value="ENSGAGG00000016421.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025261">
        <property type="entry name" value="Atos-like_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051506">
        <property type="entry name" value="ATOS_Transcription_Regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13199:SF12">
        <property type="entry name" value="ATOS HOMOLOG PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13199">
        <property type="entry name" value="GH03947P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13915">
        <property type="entry name" value="DUF4210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01177">
        <property type="entry name" value="DUF4210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Atos-like conserved" evidence="2">
        <location>
            <begin position="447"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="659" mass="69875" checksum="C34CA4519021C050" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4M9</accession>
    <name>A0A452I4M9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RNA-binding protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">RNA-binding motif protein 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I4M9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022467"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025595.1">
        <property type="protein sequence ID" value="ENSGAGP00000022467.1"/>
        <property type="gene ID" value="ENSGAGG00000016475.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12449">
        <property type="entry name" value="RRM_CIRBP_RBM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000312">
        <property type="entry name" value="RNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034278">
        <property type="entry name" value="RBM3/CIRBP_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="6"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="67"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="89"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="153" mass="16495" checksum="905426CECCF6E632" modified="2019-05-08" version="1">MSSEEGKLFVGGLNFETDEQGLEQHFSSFGPISEVVVIKDKETQRSRGFGFITFANPEHASDAMRAMNGESVDGRQIRVDHAGKSSRGSRGGSFGGRGRGRGYSRGGGDRSYGGGRYDNRSGGYGGSRDYGGRSQGGYNDRYSGGSYRDNYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4T3</accession>
    <name>A0A452I4T3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">A-type potassium channel modulatory protein DPP6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Dipeptidyl aminopeptidase-like protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Dipeptidyl peptidase 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Dipeptidyl peptidase VI</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Homodimer (in vitro). Interacts with KCND2. Identified in a complex with KCND2 and KCNIP2. Forms an octameric complex composed of four DPP6 subunits bound to the KCND2 tetramer. Interacts with KCND3; this interaction modulates the channel gating kinetics namely channel activation and inactivation kinetics and rate of recovery from inactivation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I4T3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022526"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025658.1">
        <property type="protein sequence ID" value="ENSGAGP00000022526.1"/>
        <property type="gene ID" value="ENSGAGG00000016520.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000025660.1">
        <property type="protein sequence ID" value="ENSGAGP00000022528.1"/>
        <property type="gene ID" value="ENSGAGG00000016520.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.140.10.30:FF:000001">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF20">
        <property type="entry name" value="DIPEPTIDYL AMINOPEPTIDASE-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="18">
        <location>
            <begin position="133"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="17">
        <location>
            <begin position="581"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="801" mass="91553" checksum="EF8A4F8EC44945BA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I596</accession>
    <name>A0A452I596_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCM2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I596"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022727"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025888.1">
        <property type="protein sequence ID" value="ENSGAGP00000022727.1"/>
        <property type="gene ID" value="ENSGAGG00000016662.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001570">
        <property type="term" value="P:vasculogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13516">
        <property type="entry name" value="HHD_CCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13166">
        <property type="entry name" value="PTB_CCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.20:FF:000004">
        <property type="entry name" value="Cerebral cavernous malformation 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032375">
        <property type="entry name" value="CCM2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026159">
        <property type="entry name" value="Malcavernin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21642:SF4">
        <property type="entry name" value="CEREBRAL CAVERNOUS MALFORMATIONS 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21642">
        <property type="entry name" value="CEREBRAL CAVERNOUS MALFORMATIONS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16545">
        <property type="entry name" value="CCM2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="3">
        <location>
            <begin position="62"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="410" mass="45424" checksum="2BCFC0855AF03955" modified="2019-05-08" version="1">MENEQPGIVSPFKRVFLKGEKGRDKKAQEKVTERRPLHTVLLSLPDRVEPDTLLNDYIEKEVKYLGQLTSIPGYLNPSSRTEILHLIDNAKRAHQLPGQLTQEHDAVISLSAYNVKLVWRDGEDIILRVPIHDIASVSYIRDDSSHLVVLKTAQDPGISPSQSLCAESSKALTSGSLSESGVVPVEACCLVVLATENKVTAEELCSLLSQVFQIVYTESTIDFLDRAIFDGASTPTRHMSLHSDDSSTKVDIKESYETEASTFSFPESSDTGGNSPSSFSTPQSPHVKTVSESELSTTATELLQDYMMTLRTKLSSQEIQQFAMLLHEYRNGASIHEFCINLRQLYGDSRKFLLLGKFQLSWTNIKITDLGGRGKMACGGSDSLILNPLGRVAVKPKEIMLRPSEKYSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5X6</accession>
    <name>A0A452I5X6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CELF/BRUNOL family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSGAGT00000026137.1">
        <property type="protein sequence ID" value="ENSGAGP00000022936.1"/>
        <property type="gene ID" value="ENSGAGG00000016824.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000010">
        <property type="entry name" value="CUGBP Elav-like family member 4 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="84"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="192" mass="20304" checksum="0991D25EC3772D42" modified="2019-05-08" version="1">MLYRGGKRLAAWLSPKDVRYAGCCPLLQPLVAAGRGSMATGSGGAALSTADSAARMNGLSPGPAGIAMKDHDAIKLFFGKIXGDLKPLFEEFGKIYELTVLKDRFTGMHKGCAFLTYCARDSALKAQSALHEQKTLPGVSDSSSFFGESGMAGGAESTQNPQCCALRNPVYSSWGAVPVHLQLFQGYKFSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5Z3</accession>
    <name>A0A452I5Z3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adipogenin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I5Z3"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026209.1">
        <property type="protein sequence ID" value="ENSGAGP00000022994.1"/>
        <property type="gene ID" value="ENSGAGG00000016873.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027938">
        <property type="entry name" value="Adipogenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38499">
        <property type="entry name" value="ADIPOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38499:SF1">
        <property type="entry name" value="ADIPOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15202">
        <property type="entry name" value="Adipogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7953" checksum="AAD97191F7EC5B81" modified="2019-05-08" version="1">MRYPLVPLVNDLTFPLLLFWFCLPFGLLLILLIIWLHLLLKEGKFYGQMDKCFCIKALSISFPFTALC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I692</accession>
    <name>A0A452I692_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MGAT4 family member D</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I692"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023130"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026356.1">
        <property type="protein sequence ID" value="ENSGAGP00000023130.1"/>
        <property type="gene ID" value="ENSGAGG00000016952.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006759">
        <property type="entry name" value="Glyco_transf_54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057279">
        <property type="entry name" value="MGAT4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056576">
        <property type="entry name" value="MGAT4_A/B/C_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062:SF10">
        <property type="entry name" value="ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 4-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN MGAT4D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYLTRANSFERASE VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04666">
        <property type="entry name" value="MGAT4_cons"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23524">
        <property type="entry name" value="MGAT4A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="MGAT4 conserved region" evidence="5">
        <location>
            <begin position="41"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="MGAT4 A/B/C C-terminal" evidence="6">
        <location>
            <begin position="180"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="36995" checksum="B51223399900D78A" modified="2019-05-08" version="1">SSVKTAKKYIKNYLLSGLKGKNVFSVFILSVTFFTILSFHRFFQLEDDIVAKPQYIEKIKNFALEQPSDEWMILEFSQLGFIGKLFRTKDLPLIVEFFLMFYKDKPIDWLLDHLLWVKVCSPEKDPKHCEGQKAYVRIRSKPSLFQHVGIHSSLAGKIQNLKDKDFDKNMLHKAHVNPPAKVTTSLRIFQHYTLEKAYEGMDCFWAFSPVAGDYILFTFLQPLKVDGYLFRSGNMEHPGDKLFNTTVEVWPAKLRISKSTSPIGIQRGADWFCLYIGTFDNGIAEGIISPSVGKIQAIRLSIHSDSPVWALLSEVNLKKTSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9B1</accession>
    <name>A0A452I9B1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Gamma-aminobutyric acid receptor subunit gamma-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">GABA(A) receptor subunit gamma-3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Gamma subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride across the cell membrane down their electrochemical gradient.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Heteropentamer, formed by a combination of alpha (GABRA1-6), beta (GABRB1-3), gamma (GABRG1-3), delta (GABRD), epsilon (GABRE), rho (GABRR1-3), pi (GABRP) and theta (GABRQ) chains, each subunit exhibiting distinct physiological and pharmacological properties.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG3 sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I9B1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024243"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027599.1">
        <property type="protein sequence ID" value="ENSGAGP00000024243.1"/>
        <property type="gene ID" value="ENSGAGG00000017722.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19000">
        <property type="entry name" value="LGIC_ECD_GABAAR_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19054">
        <property type="entry name" value="LGIC_TM_GABAAR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000006">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit gamma-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000003">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit gamma-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005437">
        <property type="entry name" value="GABRG-1/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01620">
        <property type="entry name" value="GABAARGAMMA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="10 23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 24" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 24" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 24" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 24" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022265579" description="Gamma-aminobutyric acid receptor subunit gamma-3" evidence="24">
        <location>
            <begin position="35"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="26">
        <location>
            <begin position="64"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="27">
        <location>
            <begin position="276"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="187"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00064898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00073032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00078610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR605437-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="385" mass="44279" checksum="EFC47C4E257669BD" modified="2019-05-08" version="1">MDSWRRPSFLWPLCSPCSRGRLLLLLLIVRLGNCADKADDEDDEDLTVNKTWVLAPKIHGGDVTHILDSLLQGYDSKLRPDIGVRTTVIETDVYVNSIGPVDPINMEYTIDIIFAQTWYDSRLKFNSSMKVLMLNSNMVGKIWIPDTFFRNSRKSDAHWITTPNRLLRIWSDGRVLYTLRLTINAECYLQLHNFPMDEHSCPLEFSSYAYPRNEIEYKWKKTSVEVADPKYWRLYQFAFVGLRNTTEISHTLSGDYIIMTIFFDLSRRMGYFTIQTYIPCILTVVLSWVSFWINKDAVPARTSLGITTVLTMTTLSTIARKSLPKVSYVTAMDLFVSVCFIFVFAALMEYGTLHYFTSNKKGNKEKEKSSKHKPAVSLSNLGKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9S0</accession>
    <name>A0A452I9S0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I9S0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024431"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027806.1">
        <property type="protein sequence ID" value="ENSGAGP00000024431.1"/>
        <property type="gene ID" value="ENSGAGG00000017850.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="82"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="429" mass="49086" checksum="44C237D86B34A16A" modified="2019-05-08" version="1">MAQYLNKYFASVFNKANEELRGNGRMTNGNEDMEVDITTSEVEAILEQLNGTKSEGPDNLHPRILKELAHEIASPLARIFNESVNSGVVPYDWRIANVVPIFKKGRKSDPSNYRPVSLTSVVCKVLEKILKEKVVKDIEVNGNWDKVQHGFTKGRSCQTNLISFFEKVTDYLDKGNAVDLIYLDFSKAFDTVPHVELLVKLEKMGINMRIERWIRNWLKGRLQRVVLKGELSGWKEVTSGVPQGSVLGPILFNLFITDLGTKSGNVLIKFADDTKLGGIANTEKDRDIIQEDLDDLVNWSNSNRMKFNSEKCKVMHLGINNKNFRYRLGMHQLEATEEEKDLGVLVDHRMTMSRQCDMAVKKANVVLGCIRRGISSKDKEVLVPLYKALVRPHLEYCVQFWSPMFKKDEFKLEQVQRRATRMIRGMENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAQ8</accession>
    <name>A0A452IAQ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EMI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IAQ8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028208.1">
        <property type="protein sequence ID" value="ENSGAGP00000024790.1"/>
        <property type="gene ID" value="ENSGAGG00000018098.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011489">
        <property type="entry name" value="EMI_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF2">
        <property type="entry name" value="EMILIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07546">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51041">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019140057" description="EMI domain-containing protein" evidence="6">
        <location>
            <begin position="28"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="EMI" evidence="7">
        <location>
            <begin position="58"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="795" mass="88465" checksum="FE250F51C31FE3B9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ID12</accession>
    <name>A0A452ID12_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ID12"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025691"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029226.1">
        <property type="protein sequence ID" value="ENSGAGP00000025691.1"/>
        <property type="gene ID" value="ENSGAGG00000018754.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043358">
        <property type="entry name" value="GNL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709:SF3">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="269"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="432" mass="49695" checksum="9DF70D209CA3C689" modified="2019-05-08" version="1">MPRKRPFSAKQKKKQLQDKRERKRGLPDGVRSSSNSRSGSHERREDHTDTSDSESLSLQVRKLNQQPAVRRLGDRSYDPNRYRLHIERESREELERRKKVAQEKILEPVPESEKELDIQDVYRSGSVLDFPKRPSWSYQMSKEQLLVREEKCFRDYLEGIYSTYQPSQLSYFEHNLETWRQLWRVLEMSDIILLITDVRHPVINFSPALYDFVTKEMRKNLILVLNKIDLAPPALVVAWKHYFQTHFPQVHVVCFTSYPQQSQDPSTVFKKRRKRRKGRSTAMGPEQLLHACETITAGKVDLSSWKEKIERDAASAHLPTEDSDEEEDNEGTAVLVEHQTDVAMDVGVPSQELYKDGVLTVGCFILLICGLGPLWTAEDWGGGSWRSWIKGAGGGVSGWRTRDLHGVFKEASEGDSVRAGGKMGGWLLWKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IE22</accession>
    <name>A0A452IE22_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IE22"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026138"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029725.1">
        <property type="protein sequence ID" value="ENSGAGP00000026138.1"/>
        <property type="gene ID" value="ENSGAGG00000019086.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028006">
        <property type="entry name" value="CEP15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14286:SF2">
        <property type="entry name" value="CENTROSOMAL PROTEIN 15 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14286">
        <property type="entry name" value="GENE, 49355-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15134">
        <property type="entry name" value="CEP15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="131" mass="15063" checksum="496B8E6A496B1DE1" modified="2019-05-08" version="1">MAFYLAQEVQLARRHDKILSQRLILLQQMENHLGDKKTEKTSQTQAADAAYKRNAALLNDIEAAEKRLQARVHLQPHPDIAKLETLYWASVEESIPKWEQFLLGRAQMPVGFKKKKSTKYNISHPEGAAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFU2</accession>
    <name>A0A452IFU2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IFU2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026821"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030484.1">
        <property type="protein sequence ID" value="ENSGAGP00000026821.1"/>
        <property type="gene ID" value="ENSGAGG00000019528.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031397">
        <property type="term" value="P:negative regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000064">
        <property type="entry name" value="Nucleoredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46472">
        <property type="entry name" value="NUCLEOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46472:SF1">
        <property type="entry name" value="NUCLEOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="186" mass="20845" checksum="392F9A68D1802FA0" modified="2019-05-08" version="1">MPWLAVPYADEARRSRLNRLYGIQGIPTLIVLDAKGDVITRQGRVEVLNDTECREFPWHPKPVLELTDSNAVQLNEGPCLVLFVDSEDDGESEAAKQLIQPIAEKIIAKYKAKEEEAPLLFFVAGEDDMTDSLRDYTNLPEAAPLLTILDMSARAKYVMDVEEITPEIVEAFVSDFLADKLKPEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGB2</accession>
    <name>A0A452IGB2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IGB2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026509"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030136.1">
        <property type="protein sequence ID" value="ENSGAGP00000026509.1"/>
        <property type="gene ID" value="ENSGAGG00000019323.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15938">
        <property type="entry name" value="7tmA_OR4Q2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000007">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="38"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="315" mass="35212" checksum="E102FF754B9A9C8F" modified="2019-05-08" version="1">ANPLQNSLSMTSVQARLSNKLAALFAVFLLVYVSTLVGNTLIMATVSSDPCLRTPMFFLLGNLSFLDLCYSTVTAPKMLLDFLSERRSISYQACMAQLFFLHFVGAAEMFLLTVMAYDRYVAICKPLHYHSIMNRTLCSWMVAASWAGGFIHSMVQTILTMRLPFCGPNWVDNFFCDVPPVIKLACTNTYVVELLMVSNSGLISTSCFVILVASYTTILVRIRSPEGRRKALSTCASHLTVVTLFFGPCIFIYARPFSIFSVDKLVSVLYNIITPMLNPLIYTLRNKEVKSAMRRLRTKGITSRGKGNGLRCRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGV2</accession>
    <name>A0A452IGV2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IGV2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027147"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030842.1">
        <property type="protein sequence ID" value="ENSGAGP00000027147.1"/>
        <property type="gene ID" value="ENSGAGG00000019744.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040429">
        <property type="entry name" value="C11orf97-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38326">
        <property type="entry name" value="CHROMOSOME 11 OPEN READING FRAME 97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38326:SF1">
        <property type="entry name" value="CHROMOSOME 11 OPEN READING FRAME 97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="121" mass="13517" checksum="301D83B658C035EE" modified="2019-05-08" version="1">MREAAGEQQQEPVAGEAGSSVCAEPAGSGHCGQPWKKFVYVEPPKRVKEVLEEELYFRRDGCQIKHPAAVALEGVWSVKRNFSMRGLTPASQNRNSLLSQPKYYSRHGGIRSKTIISRFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHD3</accession>
    <name>A0A452IHD3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IHD3"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030897.1">
        <property type="protein sequence ID" value="ENSGAGP00000027195.1"/>
        <property type="gene ID" value="ENSGAGG00000019778.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="179" mass="20236" checksum="B8E9F0FD3616A686" modified="2019-05-08" version="1">METGLGLHPGLKWECFLRYFIIFHSLCPVFHPEAGNVEILCRRPLLPGPDWLCGEQFQSIARGLRDTMFSGHWGSWEPSHRGCWLSRIPRGMLWNCSPQSQARLWGSSSPSEQTVFRARSSDVFTSWVSPWSIQHPLPLHALPTASLPRRGPGEARGSCTPTSQSDVTLSQPVKQRFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHG2</accession>
    <name>A0A452IHG2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Propionyl-CoA carboxylase beta chain, mitochondrial</fullName>
            <ecNumber evidence="3">6.4.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Propanoyl-CoA:carbon dioxide ligase subunit beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>butanoyl-CoA + hydrogencarbonate + ATP = (2S)-ethylmalonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:59520"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:60909"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:59521"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23720"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57327"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:23721"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The holoenzyme is a dodecamer composed of 6 PCCA/alpha subunits and 6 PCCB/beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AccD/PCCB family.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.3" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452IHG2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027235"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030940.1">
        <property type="protein sequence ID" value="ENSGAGP00000027235.1"/>
        <property type="gene ID" value="ENSGAGG00000019808.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000017">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit beta 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000016">
        <property type="entry name" value="Propionyl-CoA carboxylase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051047">
        <property type="entry name" value="AccD/PCCB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842:SF2">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="9">
        <location>
            <begin position="27"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="10">
        <location>
            <begin position="289"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="511" mass="55657" checksum="EA88C9A6400899A4" modified="2019-05-08" version="1">MAALRFLPRLLPVSRTRLVRSHSTSASGSVPERIAEKRRAALLGGGQARIDSQHKRGKLTARERILLLLDPDSFVEYDMFVEHRCADFGMESDQNKYPGDSVVTGQGRINGRLAYVFSQDFTVFGGSLSGAHAQKICKIMDQAVMVGAPVIGLNDSGGARIQEGVESLAGYADIFLRNVLCSGVVPQISLIMGPCAGGAVYSPALTDFTFMVKDTSYLFITGPDVVKSVTNEDVTQEQLGGAKTHTAVSGVAHRAFENDVDALLNLREFFNYLPLSNRDPAPVRKCHDPSNRLVPELDTVVPMESTKAYDMVDIVHSIVDEREFFEIMPSYARNMIVGFARMNGRTVGILGNQPKVASGCLDINSSVKGARFVRFCDAFNIPLITFVDVPGFLPGTAQEYGGIIRHGAKLLFAFAEATVPKITIITRKAYGGAYDVMSSKHLRGDVNYAWPTAEVAVMGAKGAVQIIFRGKETDAEAEYVNKFANPFPAAVRGTWDFVLQVPFLNSLVKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJF0</accession>
    <name>A0A452IJF0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trafficking protein particle complex subunit 13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the multisubunit TRAPP (transport protein particle) complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPPC13 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJF0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027990"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031793.1">
        <property type="protein sequence ID" value="ENSGAGP00000027990.1"/>
        <property type="gene ID" value="ENSGAGG00000020329.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010378">
        <property type="entry name" value="TRAPPC13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055428">
        <property type="entry name" value="TRAPPC13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055429">
        <property type="entry name" value="TRAPPC13_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055427">
        <property type="entry name" value="TRAPPC13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13134">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13134:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23643">
        <property type="entry name" value="TRAPPC13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23647">
        <property type="entry name" value="TRAPPC13_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06159">
        <property type="entry name" value="TRAPPC13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex subunit 13 N-terminal" evidence="4">
        <location>
            <begin position="10"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Trafficking protein particle complex subunit 13 middle" evidence="6">
        <location>
            <begin position="172"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Trafficking protein particle complex subunit 13 C-terminal" evidence="5">
        <location>
            <begin position="314"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="389" mass="43402" checksum="9A5A92126300D5CA" modified="2019-05-08" version="1">MDANQPKQEHLLALKVMRLTKPTLFTNIPVTCEERDLPGNLLNQLMKDDPSTVKGAETLMLGEMLTLPQNFGNIFLGETFSSYISVHNDSNQVVKDILVKADLQTSSQRLNLSASSAAVAELKSDCCIDDVIHHEVKEIGTHILVCAVSYTTQTGEKMYFRKFFKFQVLKPLDVKTKFYNAESDLSSVTDEVFLEAQIQNITTSPMFMEKVSLEPSIMYNVAELNTVNKAGESVSTFGSRTYLQPMDTRQYLYCLKPKQEFAEKAGVIKGVTVIGKLDIVWKTNLGERGRLQTSQLQRMAPGYGDVRLSLETIPDTVNLEEPFDITCKITNCSSERTMDLVLEMCNTNSIHWCGVSGRQLGKLHPSSSLHLALTLLSSVQGLQVRDITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKS1</accession>
    <name>A0A452IKS1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FERM domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IKS1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028484"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032353.1">
        <property type="protein sequence ID" value="ENSGAGP00000028484.1"/>
        <property type="gene ID" value="ENSGAGG00000020644.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17102">
        <property type="entry name" value="FERM_F1_FRMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000002">
        <property type="entry name" value="Erythrocyte protein band 4.1-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000043">
        <property type="entry name" value="FERM domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000006">
        <property type="entry name" value="FERM domain-containing protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF8">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="530"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="8">
        <location>
            <begin position="33"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="345"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="596" mass="68730" checksum="62207371103ECF61" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKU0</accession>
    <name>A0A452IKU0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alpha-crystallin B chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Alpha(B)-crystallin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May contribute to the transparency and refractive index of the lens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heteromer composed of three CRYAA and one CRYAB subunits. Aggregates with homologous proteins, including the small heat shock protein HSPB1, to form large heteromeric complexes. Inter-subunit bridging via zinc ions enhances stability, which is crucial as there is no protein turn over in the lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 11 12">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IKU0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028499"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032369.1">
        <property type="protein sequence ID" value="ENSGAGP00000028499.1"/>
        <property type="gene ID" value="ENSGAGG00000020651.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009892">
        <property type="term" value="P:negative regulation of metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000011">
        <property type="entry name" value="Alpha-crystallin B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055269">
        <property type="entry name" value="Alpha-crystallin/HSP_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003090">
        <property type="entry name" value="Alpha-crystallin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF5">
        <property type="entry name" value="ALPHA-CRYSTALLIN B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00525">
        <property type="entry name" value="Crystallin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036514">
        <property type="entry name" value="Sm_HSP_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SHSP" evidence="13">
        <location>
            <begin position="55"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036514-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="174" mass="19878" checksum="A50B0B6D8433F9BA" modified="2019-05-08" version="1">MDIAIHHPLIRRPLFSLLTPTRIFDQSFGEHLSESELFPTSGALNPFLMRFPFLRTPSWLETGFSEMRLEKDKFSVNVDVKHFSPEELKVKVLGDVIEVHGKHEERQDEHGFIAREFNRKYRIPADVDPLSITSSLSSDGVLTVNGPRKQTDVPERTIPITREEKSAIAGAQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILM2</accession>
    <name>A0A452ILM2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myosin light chain kinase 2, skeletal/cardiac muscle</fullName>
            <ecNumber evidence="4">2.7.11.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Implicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N-terminus of a myosin light chain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">May interact with centrin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.18" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452ILM2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028737"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032648.1">
        <property type="protein sequence ID" value="ENSGAGP00000028737.1"/>
        <property type="gene ID" value="ENSGAGG00000020832.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004687">
        <property type="term" value="F:myosin light chain kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000359">
        <property type="entry name" value="myosin light chain kinase 2, skeletal/cardiac muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000135">
        <property type="entry name" value="Putative myosin light chain kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="363"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="71"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="200"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="674" mass="72928" checksum="C9B970FED7E6F8B9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQX0</accession>
    <name>A0A452IQX0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Junctophilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the junctophilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IQX0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030304"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034394.1">
        <property type="protein sequence ID" value="ENSGAGP00000030304.1"/>
        <property type="gene ID" value="ENSGAGG00000021842.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030314">
        <property type="term" value="C:junctional membrane complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016529">
        <property type="term" value="C:sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000001">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000003">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000025">
        <property type="entry name" value="MORN repeat, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017191">
        <property type="entry name" value="Junctophilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085">
        <property type="entry name" value="GH28348P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085:SF26">
        <property type="entry name" value="JUNCTOPHILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037387">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="739"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="389"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="455"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="597"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="399"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="470"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="524"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="550"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="624"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="652"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="761" mass="83022" checksum="D9B562A80A06868B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRK6</accession>
    <name>A0A452IRK6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000030538"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034655.1">
        <property type="protein sequence ID" value="ENSGAGP00000030538.1"/>
        <property type="gene ID" value="ENSGAGG00000021991.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13269">
        <property type="entry name" value="PH_alsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.80:FF:000005">
        <property type="entry name" value="alsin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051984">
        <property type="entry name" value="Alsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057248">
        <property type="entry name" value="Alsin-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059093">
        <property type="entry name" value="HA_Alsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46089:SF3">
        <property type="entry name" value="ALSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46089">
        <property type="entry name" value="ALSIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25582">
        <property type="entry name" value="DH_Alsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26202">
        <property type="entry name" value="HA_Alsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25383">
        <property type="entry name" value="PH_alsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="60"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="110"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="169"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="520"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="572"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="5">
        <location>
            <begin position="683"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="domain" description="VPS9" evidence="6">
        <location>
            <begin position="1506"/>
            <end position="1650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="299"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="423"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="439"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1650" mass="182721" checksum="0EBA4500510621FD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IU31</accession>
    <name>A0A452IU31_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000031563"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035794.1">
        <property type="protein sequence ID" value="ENSGAGP00000031563.1"/>
        <property type="gene ID" value="ENSGAGG00000022664.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000011">
        <property type="entry name" value="CUB and sushi domain-containing protein 3 isoform A"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000014">
        <property type="entry name" value="Membrane cofactor protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000004">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000031">
        <property type="entry name" value="neurogenic locus notch homolog protein 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000012">
        <property type="entry name" value="neuronal pentraxin receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000225">
        <property type="entry name" value="Sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000183">
        <property type="entry name" value="Sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000018">
        <property type="entry name" value="Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051277">
        <property type="entry name" value="SEZ6_CSMD_C4BPB_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656:SF4">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="35"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 16" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 17" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="143"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="203"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="263"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="19">
        <location>
            <begin position="327"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="19">
        <location>
            <begin position="410"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="968"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1006"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1044"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1082"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1120"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1158"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="21">
        <location>
            <begin position="1199"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="1404"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="1463"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1520"/>
            <end position="1559"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="1556"/>
            <end position="1619"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="1620"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="1678"/>
            <end position="1735"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="1736"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="1794"/>
            <end position="1855"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="1856"/>
            <end position="1918"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="1919"/>
            <end position="1976"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="1977"/>
            <end position="2036"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2037"/>
            <end position="2095"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2096"/>
            <end position="2153"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2154"/>
            <end position="2211"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2212"/>
            <end position="2269"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2270"/>
            <end position="2327"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2328"/>
            <end position="2384"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2431"/>
            <end position="2487"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2488"/>
            <end position="2545"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2546"/>
            <end position="2603"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2604"/>
            <end position="2661"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2662"/>
            <end position="2719"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2720"/>
            <end position="2777"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2778"/>
            <end position="2834"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2835"/>
            <end position="2892"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2893"/>
            <end position="2951"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="2952"/>
            <end position="3011"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="3012"/>
            <end position="3069"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="3070"/>
            <end position="3127"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="3128"/>
            <end position="3185"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="3186"/>
            <end position="3243"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="3244"/>
            <end position="3302"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="20">
        <location>
            <begin position="3303"/>
            <end position="3359"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="3389"/>
            <end position="3421"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="3422"/>
            <end position="3453"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="994"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="1032"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="1070"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="1108"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="1146"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="1184"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1433"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1491"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1590"/>
            <end position="1617"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1648"/>
            <end position="1675"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1706"/>
            <end position="1733"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1764"/>
            <end position="1791"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1858"/>
            <end position="1901"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1947"/>
            <end position="1974"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="2124"/>
            <end position="2151"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="2182"/>
            <end position="2209"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="2240"/>
            <end position="2267"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="2298"/>
            <end position="2325"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="2458"/>
            <end position="2485"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="2516"/>
            <end position="2543"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="2574"/>
            <end position="2601"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="2632"/>
            <end position="2659"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="2690"/>
            <end position="2717"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="2748"/>
            <end position="2775"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="2805"/>
            <end position="2832"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="2863"/>
            <end position="2890"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="3040"/>
            <end position="3067"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="3098"/>
            <end position="3125"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="3156"/>
            <end position="3183"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="3214"/>
            <end position="3241"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="3273"/>
            <end position="3300"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="3393"/>
            <end position="3403"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="3411"/>
            <end position="3420"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="3425"/>
            <end position="3435"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="3443"/>
            <end position="3452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="3463" mass="378162" checksum="6D3581DCA77F5AAC" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUD0</accession>
    <name>A0A452IUD0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452IUD0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031720"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035972.1">
        <property type="protein sequence ID" value="ENSGAGP00000031720.1"/>
        <property type="gene ID" value="ENSGAGG00000022782.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18558">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000018">
        <property type="entry name" value="ATP-binding cassette subfamily B member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000302">
        <property type="entry name" value="ATP-binding cassette subfamily B member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF28">
        <property type="entry name" value="ATP-BINDING CASSETTE SUBFAMILY B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="20"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="352"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="593" mass="65610" checksum="69313B21801AA70C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUI7</accession>
    <name>A0A452IUI7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">hydroxymethylglutaryl-CoA lyase</fullName>
            <ecNumber evidence="3">4.1.3.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(3S)-3-hydroxy-3-methylglutaryl-CoA = acetoacetate + acetyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:24404"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="4.1.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate metabolism; (S)-3-hydroxy-3-methylglutaryl-CoA degradation; acetoacetate from (S)-3-hydroxy-3-methylglutaryl-CoA: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMG-CoA lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.4" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452IUI7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031661"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035910.1">
        <property type="protein sequence ID" value="ENSGAGP00000031661.1"/>
        <property type="gene ID" value="ENSGAGG00000022746.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000035911.1">
        <property type="protein sequence ID" value="ENSGAGP00000031662.1"/>
        <property type="gene ID" value="ENSGAGG00000022746.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00896">
        <property type="reaction ID" value="UER00863"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004419">
        <property type="term" value="F:hydroxymethylglutaryl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046951">
        <property type="term" value="P:ketone body biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07938">
        <property type="entry name" value="DRE_TIM_HMGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000038">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA lyase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043594">
        <property type="entry name" value="HMGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004283">
        <property type="entry name" value="PRK05692.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42738:SF5">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COA LYASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42738">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="7">
        <location>
            <begin position="49"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="341" mass="36496" checksum="22528B81202C24E6" modified="2019-05-08" version="1">MGTVPSALKHCLSYQHLLKEQLWIGEPAAPPPALHPGQEFQISGLPDYVKIVEVGPRDGLQNEKVTVPTDIKIELINRLSKAGLSVIEATSFVSSKWVPQMADHTEVMRRIERHPGVQYPVLTPNLQGFHSAIAAGATEVSVFGAASESFNKMNINCSIEESIEKFEEVTKLARNMNIPVRGYVSCALGCPYEGNVIPAKVVEVSKQLYSMGCYEISLGDTIGVGTPGSMKRMLEAVMKEIPVSALAVHCHDTYGQALANILTAIQMGVGVVDSAVAGLGGCPYAKGATGNVATEDVLYMLNGLGINTGVNLYKVMETGNFICTALNKKTNSKVAQASFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUS8</accession>
    <name>A0A452IUS8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IUS8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031630"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035873.1">
        <property type="protein sequence ID" value="ENSGAGP00000031630.1"/>
        <property type="gene ID" value="ENSGAGG00000022716.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46907">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46907:SF1">
        <property type="entry name" value="HEAT SHOCK PROTEIN FAMILY B (SMALL) MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="72"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="10"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="181" mass="18981" checksum="0DD40C7A5E1F351D" modified="2019-05-08" version="1">SPSSDPSPLRASTSAMASVSSSSTYRSERISTYSQNAPPAEPCFEPRGLHASVGPGAASLPLTAPFHAGSPGSVHPCSLSSNVRAGGDTYQVTADVSQFEPQDIVVTTYNYHIIIQAEKVAEDGTVSNTFTHKCQLPEDMDPMSVSCALTDAGLLVINAKRSVAAKAGEMPPPVYRTEVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IW55</accession>
    <name>A0A452IW55_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type II cytokine receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IW55"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032258"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036573.1">
        <property type="protein sequence ID" value="ENSGAGP00000032258.1"/>
        <property type="gene ID" value="ENSGAGG00000023093.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000348">
        <property type="entry name" value="Interleukin 20 receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015373">
        <property type="entry name" value="Interferon/interleukin_rcp_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050650">
        <property type="entry name" value="Type-II_Cytokine-TF_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859">
        <property type="entry name" value="INTERFERON/INTERLEUKIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859:SF51">
        <property type="entry name" value="INTERLEUKIN-22 RECEPTOR SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09294">
        <property type="entry name" value="Interfer-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01108">
        <property type="entry name" value="Tissue_fac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="11"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Interferon/interleukin receptor" evidence="6">
        <location>
            <begin position="138"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="245" mass="28456" checksum="D9DBB432BEE3C71D" modified="2019-05-08" version="1">IDIKHCRYSYLLFAVSFSLVSGTRELQELIKPQKVEFHSLNFNNTLHWHLGRAAPGDIIYFVQYKKLLKSTPSLPLHRYGQSLWKNKEECWGIREDFCDLTHQTSDIREPYYGRVKSVSAGVHSDWNTSSRFTPWRETKIGPPSVNVTPRNKSIQLKLQAPNSPYTRRRGSKIPMTNYYDLLYRVFLINNMLNEKNKILAYEGTDKVVKIEDLKPEVSYCVVVTTYLPVLDRSSVYSSKKCTVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IX27</accession>
    <name>A0A452IX27_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Metalloendopeptidase</fullName>
            <ecNumber evidence="20">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="19 20">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="19 20">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A452IX27"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032478"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036825.1">
        <property type="protein sequence ID" value="ENSGAGP00000032478.1"/>
        <property type="gene ID" value="ENSGAGG00000023233.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04281">
        <property type="entry name" value="ZnMc_BMP1_TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000022">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000004">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000007">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000009">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000011">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000014">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000004">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015446">
        <property type="entry name" value="BMP_1/tolloid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034036">
        <property type="entry name" value="ZnMP_TLD/BMP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF53">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001199">
        <property type="entry name" value="BMP_1/tolloid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 18" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 19 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 17 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 19 20" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5 19 20" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="10 17 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018808234" description="Metalloendopeptidase" evidence="20">
        <location>
            <begin position="20"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="24">
        <location>
            <begin position="115"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="22">
        <location>
            <begin position="316"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="22">
        <location>
            <begin position="429"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="23">
        <location>
            <begin position="541"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="22">
        <location>
            <begin position="585"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="22">
        <location>
            <begin position="741"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="22">
        <location>
            <begin position="854"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="82"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="active site" evidence="16 19">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17 19">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 19">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 19">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001199-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001199-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="980" mass="111226" checksum="D6F4A6331D8D3B37" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZL8</accession>
    <name>A0A452IZL8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peroxisomal biogenesis factor 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Peroxin-19</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Peroxisomal farnesylated protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with a broad range of peroxisomal membrane proteins, including PEX3, PEX10, PEX11A, PEX11B, PEX12, PEX13, PEX14 and PEX16, PXMP2/PMP22, PXMP4/PMP24, SLC25A17/PMP34, ABCD1/ALDP, ABCD2/ALDRP, and ABCD3/PMP70. Also interacts with the tumor suppressor CDKN2A/p19ARF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peroxin-19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZL8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033569"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038031.1">
        <property type="protein sequence ID" value="ENSGAGP00000033569.1"/>
        <property type="gene ID" value="ENSGAGG00000023903.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033328">
        <property type="term" value="F:peroxisome membrane targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045046">
        <property type="term" value="P:protein import into peroxisome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.900:FF:000001">
        <property type="entry name" value="Putative peroxisomal biogenesis factor 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.900">
        <property type="entry name" value="Pex19, mPTS binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006708">
        <property type="entry name" value="Pex19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038322">
        <property type="entry name" value="Pex19_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774:SF2">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04614">
        <property type="entry name" value="Pex19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="9" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019376263" description="Peroxisomal biogenesis factor 19" evidence="19">
        <location>
            <begin position="19"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="314" mass="34285" checksum="1F565C6108A291A8" modified="2019-05-08" version="1">MSILKAFCLSQCAWLPLGWLPLLPSRLCVTPPVSPSSGACMIIATQKTKPVPPPPPAAGAGDACSSETSPGDAAQHSLFASQEKFFQDLFDSELASQATAEFEKAMKELAEEEPHLVEQFQKLSEAAGRVGSDTTSQQEFTSCLKETLSGLAKNANDLQNSNMSEEELAKAMDGLGLEEGDGEGNILPIMQSIMQNLLSKDVLYPSLKEITEKYPEWLRSHRDTLPEEQFEKYQEQHSIMGKICQQFEAEQATDSDAEQKARFEMVLDLMQQLQDLGHPPKELAGESPPGLNFDLDGLNLSDTASAGGDQCRIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1W0</accession>
    <name>A0A452J1W0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J1W0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034389"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038932.1">
        <property type="protein sequence ID" value="ENSGAGP00000034389.1"/>
        <property type="gene ID" value="ENSGAGG00000024438.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22129">
        <property type="entry name" value="F-box_FBXL22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050648">
        <property type="entry name" value="F-box_LRR-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382:SF69">
        <property type="entry name" value="FI18408P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="233" mass="27090" checksum="F1C0F09A8741D819" modified="2019-05-08" version="1">MHITQLNRECLLHLFSFLDKNSRKSLAQTCHKLLEVFQEPFLWPVLNFHSPVELKKDNFLLGPALRYLSICWHSSRVKVCNIEDWMKNTFQKDICRKHENAVNHFLIQVCKRRRQSWKWCPNLLSLTLSGCGHVTDDYLLLLLKSCPNLKILKLENCVRITDQTLEAVTLYGGSLQTLHVDFCRNITQAGLETVQEKCPSVMLKAERSAHMIPDNEPGEKLMLERAVRKLVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J225</accession>
    <name>A0A452J225_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J225"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034440"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038995.1">
        <property type="protein sequence ID" value="ENSGAGP00000034440.1"/>
        <property type="gene ID" value="ENSGAGG00000024482.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05716">
        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF96">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="35"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="202" mass="23306" checksum="A55EC2ECA95F5F5D" modified="2019-05-08" version="1">MNVPRWLKCTTVSSESVLTCSLAMPLALLLAPGSPKHLMKKFGEKHQSPSEKTAMEGMSTTITCSYDRRKYMFNRKYWCRGGSRSSCDVLGDTENFVKSEYKGRVLLLDNRRGYFLVTMHQLVEEDSGMYWCGIQRPYADMMTAVKLTVTEGKDSLNIIRSHEIPRMLLSCNRQEGKRMKCRMPSGYTLASIQAIVYFYYRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J2J6</accession>
    <name>A0A452J2J6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Papilin, proteoglycan like sulfated glycoprotein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J2J6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034535"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039104.1">
        <property type="protein sequence ID" value="ENSGAGP00000034535.1"/>
        <property type="gene ID" value="ENSGAGG00000024551.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF253">
        <property type="entry name" value="DPR-INTERACTING PROTEIN ETA, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08686">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50900">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="177"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="317"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="412"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="PLAC" evidence="7">
        <location>
            <begin position="508"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="99"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="60822" checksum="FC2EF6CF30FFE29A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J2V1</accession>
    <name>A0A452J2V1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J2V1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034747"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039333.1">
        <property type="protein sequence ID" value="ENSGAGP00000034747.1"/>
        <property type="gene ID" value="ENSGAGG00000024684.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017157">
        <property type="entry name" value="Arylacetamide_deacetylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF32">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037251">
        <property type="entry name" value="Arylacetamide_deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="6">
        <location>
            <begin position="118"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="6">
        <location>
            <begin position="317"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR037251-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="410" mass="46057" checksum="CFA014A412CA3C21" modified="2019-05-08" version="1">MEFVYALLVLVLAVFIAAFILLVVGTIYFDLSNSEIPLGVDQPLKLRILHSILIGTAVLGKILEKLGLCSQLGFTRYMRRGKKLGEDPKLFIKDLQFGKVPVRIYQPRAPSAGRRRGVIYFHGGGWMFGSINSYEPICRYIARESESVVVSVEYRLAPEHTYPAQYEDCLTATTHFMKTAEDYGVDPACIIISGDSAGGNLAAAVCQTLVGRPDLPKVHAQVLIYPCLQSIDFNLPSYQQNCAVPILFRERTAFYVLQYLKGDVSFLEDVLEGSHMPVDVKLKFRKWLSADNIPKEFKVRGYKPKVPIQSSNEVYEAVKKICEPAFSPLLTEDAVVRQLPESFILTCEYDVLRDDALLYKKRLEDGGVPVTWYHIENGFHGIISLFDKGRLSFPAGKKGLDNIVNFLRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J2V3</accession>
    <name>A0A452J2V3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Centrosomal protein of 120 kDa</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Coiled-coil domain-containing protein 100</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CEP120 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J2V3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034648"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039224.1">
        <property type="protein sequence ID" value="ENSGAGP00000034648.1"/>
        <property type="gene ID" value="ENSGAGG00000024622.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022027">
        <property type="term" value="P:interkinetic nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903724">
        <property type="term" value="P:positive regulation of centriole elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000112">
        <property type="entry name" value="centrosomal protein of 120 kDa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039893">
        <property type="entry name" value="CEP120-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022136">
        <property type="entry name" value="DUF3668"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21574">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 120 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21574:SF0">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 120 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12416">
        <property type="entry name" value="DUF3668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="350"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="657"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="707"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="368"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="380"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="674"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1024" mass="117346" checksum="3E11B6132EF9F6DB" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J3B6</accession>
    <name>A0A452J3B6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J3B6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034914"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039536.1">
        <property type="protein sequence ID" value="ENSGAGP00000034914.1"/>
        <property type="gene ID" value="ENSGAGG00000024822.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24120:SF3">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24120">
        <property type="entry name" value="GH07239P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="45"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="78"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="144"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="177"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="242"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="301" mass="32545" checksum="ABC347A167F76280" modified="2019-05-08" version="1">MCKMSFKRETPLANAAFWAARKGNLALLQLLLNSGRVDVDCRDSLGTTALMVASHYGHIECVRELVLQGADINLQRESGTTALFFAAQQGHNDVVRFLFEFGASTEFRTKDGGTALLAACQYGHTRVVETLLKHGANIHDQLCDGATALFLAAQGGYLDVIRLLLSSGAKVNQPRQDGTAPLWIASQMGHSEIVRVMLLRGADRDATRNDGTTALLKAANKGYHDVIEELLKFSPALGLLKNGTSALHAAVLGGNVKAVALLLEAGADPSLRNKANELPAELTQNERILRLLRAKEKHRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J3I7</accession>
    <name>A0A452J3I7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J3I7"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039637.1">
        <property type="protein sequence ID" value="ENSGAGP00000035006.1"/>
        <property type="gene ID" value="ENSGAGG00000024885.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022181">
        <property type="entry name" value="Bcl2-/adenovirus-E1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF12">
        <property type="entry name" value="BCL2_ADENOVIRUS E1B 19 KDA PROTEIN-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12496">
        <property type="entry name" value="BNIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="189" mass="21736" checksum="A4E831FC6B21A64B" modified="2019-05-08" version="1">MEGVEFKEEWQDEDFPRPLPEDDNVEADMLAVTGQQGQHEVNGNKVRKKLMAPDISLTLDHSEESVLSDDLNESGEVDLDDLDTPSENSNEFEWEDDLPKPKTTEVMRKGSLPEYTAAEERDDGRRWRMFRIGEQDHRVDMKAIEPYKKVISHGGYYGDGLNAIVVFAVCFMPESGQPNYRYLMDNLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J463</accession>
    <name>A0A452J463_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aspartic peptidase DDI1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J463"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035225"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039885.1">
        <property type="protein sequence ID" value="ENSGAGP00000035225.1"/>
        <property type="gene ID" value="ENSGAGG00000025041.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019103">
        <property type="entry name" value="Peptidase_aspartic_DDI1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12917">
        <property type="entry name" value="ASPARTYL PROTEASE DDI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12917:SF17">
        <property type="entry name" value="NUCLEAR RECEPTOR-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09668">
        <property type="entry name" value="Asp_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aspartic peptidase DDI1-type" evidence="2">
        <location>
            <begin position="130"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="253" mass="28409" checksum="0D2AE97996468485" modified="2019-05-08" version="1">MSMRETCTQEVGPGDREEKRSEGTQNSDRRQHEVELRDKAILQQKRRLKQATQFVHKDSADLLPLDGLKRLGTSKDLQPHSVVQRRLLEGNLNKLRGESREYTTWVQSPLVKDKDDRAEENDRRKEPVSLLIHCKCQGQVLRAAVNTGCEHNLISMSCLKRLGLEEVSNAGNGDLSLPVPSVIGQVECVEVQLGQETVVCSALVVDDEMLEFCFGLQTLLSLKCCIDLEEGVLRLKALSEELPFLHASDEHGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7E5</accession>
    <name>A0A452J7E5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J7E5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036424"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041256.1">
        <property type="protein sequence ID" value="ENSGAGP00000036424.1"/>
        <property type="gene ID" value="ENSGAGG00000025831.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039190">
        <property type="entry name" value="TTC14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23184">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23184:SF9">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019494053" evidence="2">
        <location>
            <begin position="21"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="331" mass="37602" checksum="A6D71252451BB3C8" modified="2019-05-08" version="1">MPSSFVCCLLFLWLCRLSHLLTLLKKKRRRSTSSSSSSSSDSSSDVSVSSSSSSSDHKRHKKRSRNRSVSSRSSKKHSSRVSSHQRDQSRQDEWYSPPADTSASFLNQKYEMEKLLVYQKTEVRERQCSLSRTSADEEDTFGGRSEDSRDSYGSSKTQSTSSKNEKQGKADRSFSNKRGSSDSYHWKSDDKINTRDFRKLEKEVAGRTEQFRKRGSSQSMYSTSPAGSDYSGKSVEKNKQYSSTWLCEYSGSDDGSRRELTQSTNERREYKNRDSSHGEATKAKELDEETTLNGKGQSESGVKKSLPQNLLNIFNQIAEFERQKGSKQKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFJ9</accession>
    <name>A0A452GFJ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SH3 and cysteine-rich domain-containing protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Src homology 3 and cysteine-rich domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts (via SH3 domains) with CACNA1S. Interacts with CACNA1H. Interacts with CACNA1C.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GFJ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000341"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000385.1">
        <property type="protein sequence ID" value="ENSGAGP00000000341.1"/>
        <property type="gene ID" value="ENSGAGG00000000290.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903078">
        <property type="term" value="P:positive regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11833">
        <property type="entry name" value="SH3_Stac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000044">
        <property type="entry name" value="SH3 and cysteine-rich domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000073">
        <property type="entry name" value="SH3 and cysteine-rich domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039688">
        <property type="entry name" value="STAC1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035508">
        <property type="entry name" value="STAC1_SH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135:SF3">
        <property type="entry name" value="SH3 AND CYSTEINE-RICH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135">
        <property type="entry name" value="STAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16664">
        <property type="entry name" value="STAC2_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01887">
        <property type="entry name" value="SPECTRNALPHA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="17">
        <location>
            <begin position="86"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="265"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="190"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="367" mass="41805" checksum="47C906D743417018" modified="2019-05-08" version="1">MCLKKKVTNSSVIALQLQKLKRSLSFKTKSLRSKSADNFFQRTNSDMKLQVDLLPEVSTNIGQLPNSESQTSTPIKLQQQSESNQVHVFQEHIFKKPTFCDVCNHMIVGTNAKHGLRCKACKMSIHHKCTDNIGQQRCMGKLPKGFRRYYSSPLLIHEQFGCIKEVMPIACGNKVDPVYETLRFGTSLAQKTKRGSSGSGSDSPKRNSTSDLAEVPEEADGSGRMFDINRKRSNSVFTYSENGTEDFGEESKRINSLGPLFKDTLQMHTYVALYKFVPQENEDLEMRPGDMITLLEDSNEDWWKGRIEDRIGFFPANFVQRVQQDEKIFKCVRTFIGCKEQGQITLKENQVSEVHHLLYIKDTVLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFR8</accession>
    <name>A0A452GFR8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCDC90 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GFR8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000394"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000439.1">
        <property type="protein sequence ID" value="ENSGAGP00000000394.1"/>
        <property type="gene ID" value="ENSGAGG00000000196.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051561">
        <property type="term" value="P:positive regulation of mitochondrial calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000015">
        <property type="entry name" value="Mitochondrial calcium uniporter regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF11">
        <property type="entry name" value="MITOCHONDRIAL CALCIUM UNIPORTER REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="277" mass="30465" checksum="93A931CBAC67D8CC" modified="2019-05-08" version="1">PGPRAPASNRRLAPPGDAAPGAMLWSKGISGLGAAAAAGTGRLRHCGARGERGELGGLASAAARLGEGDVKGKFSLSGSRKLFFDTHALVCLLEENGFTTQQSEIIVSALVKIMNTNMDVFYKDMVTKVQQEITLQQIMSHIGGVKKDMIILEKSEFSALRAENEKIKLELYLLKKQVMDEVTKVRADNKLGFNLEKSRVKELYSINEKKLLEIRTEIVAMHAQQDRALTQTDRKIDTEVAGLKTMLESHKLDNIKYLAGSVFTCLTVALGFYRLWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGN7</accession>
    <name>A0A452GGN7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3 Na(+)(out) + phosphate(out) = 3 Na(+)(in) + phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:71255"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily. Sodium/anion cotransporter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GGN7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000744"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000835.1">
        <property type="protein sequence ID" value="ENSGAGP00000000744.1"/>
        <property type="gene ID" value="ENSGAGG00000000618.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17318">
        <property type="entry name" value="MFS_SLC17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000003">
        <property type="entry name" value="Solute carrier family 17 member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000060">
        <property type="entry name" value="Solute carrier family 17 member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF284">
        <property type="entry name" value="SMALL INTESTINE URATE EXPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="14">
        <location>
            <begin position="47"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="472" mass="51730" checksum="AAD3668DD14B22B5" modified="2019-05-08" version="1">MQQSAENAEAEDCPESDQEDAAPLLNQQLSLGKGLCSARYSLALILHFSLFMVYSLRVNLSIAIVAMTNCTNLGSLPNISTDVCPNHSHAPMYNWSTKTQGVILSAFFYGYLFTQLLGGYWSGMFGGKLVLGCGLLSTSALTLLAPLAASLGAVYLIGLQAVLDMAEGVIFPAQYTLWAKWAPPLECSRLMNLAGAGCTFGTFITFPAARIICQYLGWPYIFFIFSGIGCVWFVFWFLLVYEDPAHHPRISAGEKEYVVSSLANQGSSHGRSLPLLAVMKSLPLWAIAVTYFWLFYMLLMSMPTYMNDVLHFDIRENGLLSALPYMGNWLGTIVSGLLADFLLSKHMFSTAMVRKLFSMLGMMLPAVSLLAVSYVGCDYTAAVVFLTLSMTIFSSLLPRYVGFLLGVTNIFGTVLGIIAPTILITPCLVPPQTGWRNVFFVSAALNLFGLLFYVTFGSGTIQDWAREDTALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGV4</accession>
    <name>A0A452GGV4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000810"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000913.1">
        <property type="protein sequence ID" value="ENSGAGP00000000810.1"/>
        <property type="gene ID" value="ENSGAGG00000000682.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="CDD" id="cd14617">
        <property type="entry name" value="R-PTPc-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000369">
        <property type="entry name" value="Protein tyrosine phosphatase, receptor type B"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001376">
        <property type="entry name" value="Protein tyrosine phosphatase, receptor type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000009">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041201">
        <property type="entry name" value="PTPRJ_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF2">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24562">
        <property type="entry name" value="CysR_MRC2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18861">
        <property type="entry name" value="PTP_tm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019105789" description="protein-tyrosine-phosphatase" evidence="14">
        <location>
            <begin position="21"/>
            <end position="2211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1830"/>
            <end position="1856"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="326"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="505"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="683"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="856"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="944"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="1123"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="1389"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="1477"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="1571"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="15">
        <location>
            <begin position="1917"/>
            <end position="2177"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="16">
        <location>
            <begin position="2095"/>
            <end position="2168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2211" mass="248807" checksum="0F12C679A5D70E1E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIF8</accession>
    <name>A0A452GIF8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GIF8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001433"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001621.1">
        <property type="protein sequence ID" value="ENSGAGP00000001433.1"/>
        <property type="gene ID" value="ENSGAGG00000001083.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000604">
        <property type="entry name" value="immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000674">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000689">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000932">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000491">
        <property type="entry name" value="Immunoglobulin superfamily, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051102">
        <property type="entry name" value="IgSF_V-set/TM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207:SF21">
        <property type="entry name" value="IMMUNOGLOBULIN SUPERFAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="992"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="29"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="181"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="292"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="445"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="562"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="699"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="837"/>
            <end position="973"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1062" mass="119971" checksum="396A5C7CEF48A9D0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIJ1</accession>
    <name>A0A452GIJ1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RNA-binding protein 38</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">RNA-binding motif protein 38</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the RBM38 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GIJ1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001476"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001683.1">
        <property type="protein sequence ID" value="ENSGAGP00000001476.1"/>
        <property type="gene ID" value="ENSGAGG00000001187.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12384">
        <property type="entry name" value="RRM_RBM24_RBM38_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000524">
        <property type="entry name" value="RNA-binding motif protein 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050886">
        <property type="entry name" value="RNA-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024">
        <property type="entry name" value="GEO13361P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024:SF28">
        <property type="entry name" value="RNA-BINDING PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="11"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="215" mass="23674" checksum="FE236FE41302A638" modified="2019-05-08" version="1">MHTVQKDTTFTKIFVGGLPYHTTDSSLRKYFEVFGDIEEAVVITDRQTGKSRGYGFVTMADRAAAERACKDPNPIIDGRKANVNLAYLGAKPRSIQTGFTIGVQQLHPAFIQRPYGLTPHYIYPQAIVQPSVVIPTPVQSLTSPYIDYTSASQAYTQYTTAAYDQYPYAASPAAGFVGYGYTSAVQQPITASNPAAHTTAFVQYQPQQLQPDRMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKE9</accession>
    <name>A0A452GKE9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Acyl-CoA dehydrogenase 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>decanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-decenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:48177"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dodecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-dodecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47296"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:47297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:43465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>octanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-octenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48180"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:48181"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>oxidized [electron-transfer flavoprotein] + hexadecanoyl-CoA + H(+) = (2E)-hexadecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:43449"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tetradecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-tetradecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:47317"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GKE9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002187"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002494.1">
        <property type="protein sequence ID" value="ENSGAGP00000002187.1"/>
        <property type="gene ID" value="ENSGAGG00000001768.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF6">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="18">
        <location>
            <begin position="52"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="17">
        <location>
            <begin position="168"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="16">
        <location>
            <begin position="275"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="47483" checksum="6B25E314660A5F41" modified="2019-05-08" version="1">MGRCWPGLLTRGERGWWSSGLPGPARSQLRAASSSRAPGATGEGANLLYSGEHNALRASLRKIIDKEINPFVDKWEEEGQFPAHKVFKTLGQAGFLGVNKPIEYGGMGLDFSYSIAVAEELGNIHCGGVPMAIGVQTDMATPALTRFGSDELKRQFLVPTIAGEVVACLGVSEPGAGSDVASIKTTAVRKGDDYIINGGKMWITSGCQADWMCLLANSSEGPRYQNKSLICLPMNLPGVHIAKKIDKMGMRSSDTAQIFFEDVRVPSKYLIGEEGMGFTYQMLQFQEERLWAVANILAPMENVIQETIDYTRQRKIYNQPVLHNQTVHFRLAELATEVELVRSLFYQAVALYVRGNDVTKFASMAKLKEGRLARELSDNCLQFWGGMGFTSEVLVNRLYRDSRLLSRGAGADEVMLSIICKYMETLPQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKL5</accession>
    <name>A0A452GKL5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GKL5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002125"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002419.1">
        <property type="protein sequence ID" value="ENSGAGP00000002125.1"/>
        <property type="gene ID" value="ENSGAGG00000001714.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF193">
        <property type="entry name" value="LOC571647 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="206"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="421" mass="46278" checksum="79E9240B36242457" modified="2025-10-08" version="2">MCWRGGCGGPLPALSPSPAGAAPDGPHSLAYHYVGTYDAAGLLEFGAVGLLDGAPIDGYDRAARAKVPAQPWVGEGLGPEYWRQGGASRAAKEDWFRRNVETLKQRSNQTQGRHTLQWALGCEREPGGVVRGWYQFGYDGQDLVVFDRGNRRWLAALSWAETTRRRWDAQPEFGERLHRYLGDTCVEWLGRFLRSRQAWARRAAAPQLQAWSRPTPGRPGWLTLGCQTWGFPTRDIEVSWLRGDAMLPGAEAGAGLPSGDGCYQRQSHLRVPEREAQGVRCRARHGDLEMPLETTWAPRSPSYRGGVSPGTVAGATLGALGGLAGALLLCRRRGKRGGGRSWAENGHNSSPDLTHRLSPQLAGSWDSPGCTWAQRKRRPNSADPVGLVGFSTRMPGFLATQGWTREWSWKGAWPLRTWGPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLC5</accession>
    <name>A0A452GLC5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RuvB-like helicase</fullName>
            <ecNumber evidence="11">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Proposed core component of the chromatin remodeling Ino80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Dynein axonemal particle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A452GLC5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002575"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002945.1">
        <property type="protein sequence ID" value="ENSGAGP00000002575.1"/>
        <property type="gene ID" value="ENSGAGG00000002065.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000001">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 11" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="62"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="456" mass="50171" checksum="2943852C39A84392" modified="2019-05-08" version="1">MKIEEVKSTSKTQRIAAHSHVKGLGLDESGAAKPAGAGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYATEFDLEAEEYVPLPKGDVHKKKEIIQDVTLHDLDVANARPQGGQDILSMMGQLMKPKKTEITDKLRGEINKVVNKYIDQGIAELVPGVLFVDEVHMLDIECFTYLHRALESSIAPIVIFASNRGNCIIRGTEDIVSPHGIPLDLLDRVMIIRTMLYTPQEMKQIIKLRAQIEGINISEEALNHLGEIGNKTTLRYAVQLLTPANLLAKINGKDSIEKEHIEEINELFYDAKSSAKILADQQEKYMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMI1</accession>
    <name>A0A452GMI1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMI1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003032"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003472.1">
        <property type="protein sequence ID" value="ENSGAGP00000003032.1"/>
        <property type="gene ID" value="ENSGAGG00000002424.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF46">
        <property type="entry name" value="PROTOCADHERIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="12">
        <location>
            <begin position="27"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="12">
        <location>
            <begin position="113"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="224" mass="23860" checksum="960FA0A7C63E00CD" modified="2019-05-08" version="1">GWCTWSWRCGTSTTTAAPGTRLPLEIALDPDVGSNSIQSFQLSRNSHFGIEAQPRADGGKSAELVLLHELDRETQAAYTLELVAQDGGSPARAGTATVSVRVLDANDNSPAFPQGSLTVELGEDAPRGSLLLDLDAADPDEGPNGEIVYGFGSQVPAEARQLFRLDPLSGRLTLEGPVDYERQRTYELDVQAQDRGASPLAATCKVIVTWARRPARPSGSARCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMP7</accession>
    <name>A0A452GMP7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FXYD domain-containing ion transport regulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the FXYD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMP7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002956"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003389.1">
        <property type="protein sequence ID" value="ENSGAGP00000002956.1"/>
        <property type="gene ID" value="ENSGAGG00000002361.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000003391.1">
        <property type="protein sequence ID" value="ENSGAGP00000002958.1"/>
        <property type="gene ID" value="ENSGAGG00000002361.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017080">
        <property type="term" value="F:sodium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043269">
        <property type="term" value="P:regulation of monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20324">
        <property type="entry name" value="FXYD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.780">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047297">
        <property type="entry name" value="FXYD_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000272">
        <property type="entry name" value="Ion-transport_regulator_FXYD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14132:SF12">
        <property type="entry name" value="PHOSPHOLEMMAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14132">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02038">
        <property type="entry name" value="ATP1G1_PLM_MAT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01310">
        <property type="entry name" value="FXYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="28"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="155" mass="16752" checksum="A15131D5E5FD39B6" modified="2019-05-08" version="1">QNHTHPPRGCFVPPHPDPAVGGSYEILNCHPPPPSSGPTPTAWSPPPFPQSHPRPAPGRAAGGALPAVRSRTLRSARLTAARGAGTDMGTWILLLLCWALLGAASAEPPKEHDPFNYDYQSLRIGGLIFAVILFLLGILIILSRRCRCKFNQQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GN50</accession>
    <name>A0A452GN50_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GN50"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003275"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003774.1">
        <property type="protein sequence ID" value="ENSGAGP00000003275.1"/>
        <property type="gene ID" value="ENSGAGG00000002648.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15222">
        <property type="entry name" value="7tmA_OR51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000002">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF104">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="43"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="319" mass="35356" checksum="BBC352070D745B90" modified="2019-05-08" version="1">MAAFNHTSASSAVFLLMGIPGLEQAHGWLAIPLCSLYIVAVLGNCIILFIIRAELSLHQPMYYFLSMLALTDLGLSASTLPTMLSIFLLNSRKVNADACFAQLYFIHTFSVMESAVLLAMAFDCFVAIHKPLRYASILTNATVARIGLVLMARSVVMVMPTPILLRRLRYCLPNVLSPSFCLHQDVMKLACSNRMINSIYGLFVVIITMGLDALLIVLSYVMIITTVLSIASKEECLKALNTFVSRICAVLIFYIPMIGISMIHRFEESASPLVPVLMAYVYVLVPPMMNPIVYSVKTKQICWGLLLGLCECLLLGYWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQ45</accession>
    <name>A0A452GQ45_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GQ45"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003819"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004422.1">
        <property type="protein sequence ID" value="ENSGAGP00000003819.1"/>
        <property type="gene ID" value="ENSGAGG00000003107.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004967">
        <property type="term" value="F:glucagon receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF23">
        <property type="entry name" value="GLUCAGON-LIKE PEPTIDE 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="17748" checksum="A7F881218CDBEB10" modified="2019-05-08" version="1">EFIFTFITDEQVEGLSRHIRLFMQLTMSSFHGFLVAILYCFVNGEVKAELQKQWSRFLLADPFGCMQCFLGENIKYLGKYSKKQKHQHFANNRLCVEVSEPWAAQLQQVLLNGRADLSSEPGPTGEPYPRASVSDISEGEVTTGETTEEVFEESEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQC4</accession>
    <name>A0A452GQC4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 19">Polyprenal reductase</fullName>
            <ecNumber evidence="3 19">1.3.1.22</ecNumber>
            <ecNumber evidence="4 19">1.3.1.94</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a key role in early steps of protein N-linked glycosylation by being involved in the conversion of polyprenol into dolichol. Acts as a polyprenal reductase that mediates the reduction of polyprenal into dolichal in a NADP-dependent mechanism. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>17beta-hydroxy-5alpha-androstan-3-one + NADP(+) = testosterone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50820"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16330"/>
            <dbReference type="ChEBI" id="CHEBI:17347"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:50822"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 3-oxo-5alpha-steroid + NADP(+) = a 3-oxo-Delta(4)-steroid + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54384"/>
            <dbReference type="ChEBI" id="CHEBI:13601"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:47909"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:54386"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>a di-trans,poly-cis-dolichal + NADP(+) = a di-trans,poly-cis-polyprenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80727"/>
            <dbReference type="Rhea" id="RHEA-COMP:19536"/>
            <dbReference type="Rhea" id="RHEA-COMP:19537"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:231623"/>
            <dbReference type="ChEBI" id="CHEBI:231637"/>
            <dbReference type="EC" id="1.3.1.94"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18 19">
            <dbReference type="Rhea" id="RHEA:80729"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>androst-4-ene-3,17-dione + NADPH + H(+) = 5alpha-androstan-3,17-dione + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:50816"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15994"/>
            <dbReference type="ChEBI" id="CHEBI:16422"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:50818"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 19">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 19">Belongs to the steroid 5-alpha reductase family. Polyprenal reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.22" evidence="3 19"/>
    <dbReference type="EC" id="1.3.1.94" evidence="4 19"/>
    <dbReference type="AlphaFoldDB" id="A0A452GQC4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004138"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004837.1">
        <property type="protein sequence ID" value="ENSGAGP00000004138.1"/>
        <property type="gene ID" value="ENSGAGG00000003410.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047751">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160198">
        <property type="term" value="F:polyprenal reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102389">
        <property type="term" value="F:polyprenol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016095">
        <property type="term" value="P:polyprenol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000021">
        <property type="entry name" value="Polyprenol reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039698">
        <property type="entry name" value="Dfg10/SRD5A3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624">
        <property type="entry name" value="DFG10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624:SF0">
        <property type="entry name" value="POLYPRENOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 19" id="KW-0521">NADP</keyword>
    <keyword evidence="9 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019461420" description="Polyprenal reductase" evidence="20">
        <location>
            <begin position="20"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="21">
        <location>
            <begin position="190"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU367081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="307" mass="35031" checksum="D484B7D6B2DCB883" modified="2019-05-08" version="1">MVSAISALWLLLAAAFLAALLLELLQAPRAGLGARLFQDLIRYGKTKSGCGQRPAWLRLLDVPKRWFSHFYILSVVWNSFLLLLLIQALFLSRPFPVWLQNLLSALGGASQNQDVGDKHLSVLLVLMLLLLHSFQRLIECLYISVFSSGVLHILQYCMGLGYYIVIGLTVLCQVPANVSNGKDLFMQICWYHILGIMMYIWASVHQHRCHVILANLRKGKSGKVVSLNHSIPFGDWFERVSCPHYFAELLIYVSMAVIFGFQNLTWWLVVMFVLFNQALAAVLCHEFYVNKFSCYPTHRKAFIPFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRP2</accession>
    <name>A0A452GRP2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome c oxidase assembly protein COX19</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the COX19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GRP2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004615"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005405.1">
        <property type="protein sequence ID" value="ENSGAGP00000004615.1"/>
        <property type="gene ID" value="ENSGAGG00000003812.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051383">
        <property type="entry name" value="COX19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21107">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21107:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHCH" evidence="7">
        <location>
            <begin position="30"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="93" mass="10857" checksum="C1C043F81AF1135E" modified="2019-05-08" version="1">MSTAMNFGSKSFTPRPPDKGAFPLDHFGECKVFKEKFMKCLRENSFENASCRLESKEYLECRMERQLMAKEPLEKLGFKDLTDEKSEAKHKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSX0</accession>
    <name>A0A452GSX0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">6-phosphogluconolactonase</fullName>
            <shortName evidence="6">6PGL</shortName>
            <ecNumber evidence="4 6">3.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="3.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.31" evidence="4 6"/>
    <dbReference type="AlphaFoldDB" id="A0A452GSX0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005096"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005943.1">
        <property type="protein sequence ID" value="ENSGAGP00000005096.1"/>
        <property type="gene ID" value="ENSGAGG00000004144.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00409"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000005">
        <property type="entry name" value="6-phosphogluconolactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF0">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="7">
        <location>
            <begin position="14"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="254" mass="27343" checksum="FF3B536EDBDAE0BA" modified="2019-05-08" version="1">MPGFVPRHISVFPSPQELGSSLAQLVAQRAAESGGRFSLGLSGGSLVRILSQELPEAAALSGPAAPARWVLAFCDERLVPVEDPESTYGAYKTHLLSKISIPEKQVVIINPSLPVEEAASDYAEKLKEAFQGENMPVFDLLILGIGPDGHTCSLFPDHPLLQEKEKIVASISDSPKPPPERITLTLPVLNAARTVVFVATGESKAAILKRILEGDEENPLPAALVQPHTGKLHWFLDESAAKELTVPFEKHSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GT55</accession>
    <name>A0A452GT55_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">PAS domain-containing serine/threonine-protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005201"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006062.1">
        <property type="protein sequence ID" value="ENSGAGP00000005201.1"/>
        <property type="gene ID" value="ENSGAGG00000004225.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000059">
        <property type="entry name" value="PAS domain containing serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000351">
        <property type="entry name" value="PAS domain-containing serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000346">
        <property type="entry name" value="PAS domain-containing serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF51">
        <property type="entry name" value="PAS DOMAIN-CONTAINING SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="23">
        <location>
            <begin position="124"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="1021"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="530"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="1050"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00071822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1342" mass="147638" checksum="52158602E590C188" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GT85</accession>
    <name>A0A452GT85_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Cysteine protease</fullName>
            <ecNumber evidence="19">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylserine + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phospho-L-serine</text>
            <dbReference type="Rhea" id="RHEA:67576"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="Rhea" id="RHEA-COMP:17326"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172942"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:67577"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 19">Belongs to the peptidase C54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A452GT85"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005223"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006085.1">
        <property type="protein sequence ID" value="ENSGAGP00000005223.1"/>
        <property type="gene ID" value="ENSGAGG00000004241.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000006089.1">
        <property type="protein sequence ID" value="ENSGAGP00000005227.1"/>
        <property type="gene ID" value="ENSGAGG00000004241.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046793">
        <property type="entry name" value="ATG4_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF39">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20166">
        <property type="entry name" value="ATG4_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 19" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 19" id="KW-0645">Protease</keyword>
    <keyword evidence="13 19" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="20">
        <location>
            <begin position="39"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="393" mass="44528" checksum="E6535250F6D36D40" modified="2019-05-08" version="1">MDAATLTYDTLRFEYEDFPETKEPVWILGRTYSVFTEKEEILLDVTSRLWFTYRKNFPAIGGTGPTSDTGWGCMLRCGQMIFAQALVCQHLGRDWRWEKGKRQTDNYFNILNAFIDKKDSYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLATFDTWSSLAVHVAMDNTVVMEEIRRLCKSNFLCAGAAAFPAAESDLLYNGFPDGAEVTDRLSQWKPLVLLIPLRLGLTDINEAYIETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAMEPSDNGCIPDESFHCQHPPCRMSIAELDPSIAVGFFCNTEEDFNNWCQQIKKLSLLRGALPMFELVEHQPSHFSSPDVLNLTPDSSDADRLERFFDSEDEDFEILSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTI8</accession>
    <name>A0A452GTI8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTI8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006204.1">
        <property type="protein sequence ID" value="ENSGAGP00000005334.1"/>
        <property type="gene ID" value="ENSGAGG00000004317.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15911">
        <property type="entry name" value="7tmA_OR11A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF397">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="44"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="317" mass="35703" checksum="1050D1C7142378AF" modified="2019-05-08" version="1">MAGLEKRNHTQITEFLLVGFETFPELQITLFVVFFIIYLGTVAGNLLLILTVWTDHHLHTPMYFFLSNLSFLETGYISNIIPRLLVSLATGDKTISLLGCFLQLFVFSFLGATECFLLTGMSYDRYLAICDPLHYASNMSPRASFWMAFASWTLGFVASFFTIIMASMLPFCGPHEINHFFCDLTAVLKLPCTDTSLIEMTALISSSTVLVIPFALTIMSYVYIILTILRLPSNNGRKKAFSTCSSHLIVVSTFYGALIIMYVVPSGNQSLDFNKAFSLLYIVVTPMLNPIVYSLRNQEVKDALSRSLTKMWYSIKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVT4</accession>
    <name>A0A452GVT4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cell membrane</location>
            <topology evidence="1 15">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GVT4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006151"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007160.1">
        <property type="protein sequence ID" value="ENSGAGP00000006151.1"/>
        <property type="gene ID" value="ENSGAGG00000002204.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099560">
        <property type="term" value="P:synaptic membrane adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000297">
        <property type="entry name" value="Cadherin 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000012">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000017">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000014">
        <property type="entry name" value="Cadherin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000435">
        <property type="entry name" value="Cadherin 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000006">
        <property type="entry name" value="Cadherin-9 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF99">
        <property type="entry name" value="CADHERIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 14 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 15" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019562813" description="Cadherin domain-containing protein" evidence="17">
        <location>
            <begin position="23"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="582"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="51"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="138"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="247"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="362"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="466"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="767" mass="85429" checksum="6FA230ADC7E178EE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWF4</accession>
    <name>A0A452GWF4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GWF4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006340"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007371.1">
        <property type="protein sequence ID" value="ENSGAGP00000006340.1"/>
        <property type="gene ID" value="ENSGAGG00000002943.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08849">
        <property type="entry name" value="ArfGap_ASAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13251">
        <property type="entry name" value="PH_ASAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000002">
        <property type="entry name" value="arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.950:FF:000001">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000006">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000012">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043593">
        <property type="entry name" value="ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037844">
        <property type="entry name" value="PH_ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854:SF4">
        <property type="entry name" value="ARF-GAP WITH SH3 DOMAIN, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854">
        <property type="entry name" value="ASAP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="146"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="14">
        <location>
            <begin position="262"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="425"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="461"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="786"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="541"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="705"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="711"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="861" mass="96796" checksum="FE471BCD60B38D9F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWZ0</accession>
    <name>A0A452GWZ0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GWZ0"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007719.1">
        <property type="protein sequence ID" value="ENSGAGP00000006642.1"/>
        <property type="gene ID" value="ENSGAGG00000005367.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="54" mass="5856" checksum="FF86E902405E4963" modified="2019-05-08" version="1">ISRTITRIRAVPDRGGEPPSIAMSVKLISSFSSRSRGLSSTSSAYFLLSLRSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GX56</accession>
    <name>A0A452GX56_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Negative elongation factor E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">RNA-binding protein RD</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">The NELF complex is composed of NELFA, NELFB, NELFCD and NELFE. Interacts with NELFB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRM NELF-E family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GX56"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006710"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007790.1">
        <property type="protein sequence ID" value="ENSGAGP00000006710.1"/>
        <property type="gene ID" value="ENSGAGG00000005411.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032021">
        <property type="term" value="C:NELF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034244">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12305">
        <property type="entry name" value="RRM_NELFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000188">
        <property type="entry name" value="Negative elongation factor complex member E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033102">
        <property type="entry name" value="NELFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034637">
        <property type="entry name" value="NELFE_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17250">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17250:SF0">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="12 20" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="22">
        <location>
            <begin position="211"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="88"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="155"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00059578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00063939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="321" mass="36370" checksum="A9BFA0B2F73E311E" modified="2019-05-08" version="1">MLVLPPALTEEEEALQKKFAKLKKKKKALLALKKQQSSTSQASQGGIKRSISDQPVVDTATATEQAKMLVKTGAISAIKAENKNCGFKRSRTLEGKLKDPEKGPAPTFQPFQRSLSADDECQESRRPQRKSLYESFVSSSERLRDPEKEPEDERDLEKEPERGEGLHTFEWDYDRDRSRERSRERDRDRERDSSFRRSDSFPDRRAPRKGNTVYVYGTDMTQSLLRGAFSPFGTIIDLSMDNPRNCAFVTYEKMESADQAIAELNGAVVEDVQLKVSIARKQPMLDAATGKSVWGSLAVKNSAKGSHRDRRPQVVYNEDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXV0</accession>
    <name>A0A452GXV0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">unspecific monooxygenase</fullName>
            <ecNumber evidence="5">1.14.14.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452GXV0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006981"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008088.1">
        <property type="protein sequence ID" value="ENSGAGP00000006981.1"/>
        <property type="gene ID" value="ENSGAGG00000005622.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008392">
        <property type="term" value="F:arachidonate epoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019373">
        <property type="term" value="P:epoxygenase P450 pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF356">
        <property type="entry name" value="CYTOCHROME P450 2E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="399" mass="46069" checksum="B3E690394538C99B" modified="2019-05-08" version="1">TRVRLRLLLCSVKLTYPPRWRSRKDNPYSFLFLSGFSKNVLVLCLNLLQLSVKYGPIFTIYLGSLRVVVLYGQEAVKEALIDQGDEFSGRGKLALADKLAKGTGIVFSNGEQWKQLRRFALTTLRNFGMGKKSVEERIQEEARFLVERLRNTHERPFDPTLPLTHAVSNVICSIVFGDRFDYEDQKFLTLIHLTEENNELLRSLLAQVCSLYNFFPTLMDYIPGPHQELLKNSEEFRIFVLERVKMHKESLDLSCPRDFIDAFLIKMEQEQQNDQSEFNTENLVRSTLDLFLAGTGTTSTTLRHGLLILLKYPDIEEKVHEEIDHVIGRSRSPCMADRSQMPYTDAVIHEIQRFINLVPLNLPHAVTRDTHFRQYVIPKVSSQCFSEKSSSSTSSFFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZ96</accession>
    <name>A0A452GZ96_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom40 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZ96"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007508"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008672.1">
        <property type="protein sequence ID" value="ENSGAGP00000007508.1"/>
        <property type="gene ID" value="ENSGAGG00000006008.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07305">
        <property type="entry name" value="Porin3_Tom40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000005">
        <property type="entry name" value="mitochondrial import receptor subunit TOM40 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037930">
        <property type="entry name" value="Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10802">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="342" mass="37374" checksum="C2D0027B7880D7B8" modified="2019-05-08" version="1">MGNVLAPVLDLHRPLRREEPLSNPGSFDELHRQCKEVFPQQMEGVKLIINKALSSHFQVTHTIHMSTVGQSSYHFNTTYVGDRQLSPTETFPMLVGDIDSCGSLNAQVLHLVAERIRTKAVFQTQQARFVTWQFDGELRGDDYTATLTLGNPDMLSQSVIVVAHFLQSVTSRLVLGGEMVYHRRPGEEGAIVTLAGKYTALKWVATLNIGYGGAHASYYHRANEQVQVGVELEANTRLQDTTFAFGYQLNLPQANMVFRGECGAGPAPTQAGGCPGLCQPRPGGKASPQPLDPQTASVHSARRCKITPTPAWLYGPAGSQLVLGLRMFIAGVFPTPLRCFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H080</accession>
    <name>A0A452H080_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H080"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007875"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009077.1">
        <property type="protein sequence ID" value="ENSGAGP00000007875.1"/>
        <property type="gene ID" value="ENSGAGG00000006265.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000249">
        <property type="entry name" value="Zinc finger protein 154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002005">
        <property type="entry name" value="Zinc finger protein 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000352">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000023">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001437">
        <property type="entry name" value="Zinc finger protein 594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408:SF34">
        <property type="entry name" value="ZINC FINGER PROTEIN 672-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="355"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="383"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="411"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="439"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="467"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="495"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="523"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="553" mass="61731" checksum="E90423A8A4859121" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1M1</accession>
    <name>A0A452H1M1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Threonine synthase like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H1M1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008354"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009619.1">
        <property type="protein sequence ID" value="ENSGAGP00000008354.1"/>
        <property type="gene ID" value="ENSGAGG00000006640.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43515">
        <property type="entry name" value="THREONINE SYNTHASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43515:SF1">
        <property type="entry name" value="THREONINE SYNTHASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019484527" description="Threonine synthase like 1" evidence="3">
        <location>
            <begin position="25"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="27978" checksum="DF88E004222F477E" modified="2019-05-08" version="1">MERSSRRLLCSFVLIISSCVQILTTPKHHTEFLLCNSFIHPADIPAFKLGEIVEIAYGENFTCSKIAPLRYLTGNQFLLELFHGPTASFKDLALQLMPHLFAYCIPKRCNYLVLVATSGDTGSAVLDGFSRLSDTDKQRIAVVTFFPEDGVSQIQKSQMISCQRENGWSVGIRSDFDFCQTAIKEIFTNSDYSGFLTVEYGTALSAANSINWARLLPQVIYHASAYLDLVHQDIITFGRELVALKHSYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1U4</accession>
    <name>A0A452H1U4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Helicase with zinc finger</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000008468"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009749.1">
        <property type="protein sequence ID" value="ENSGAGP00000008468.1"/>
        <property type="gene ID" value="ENSGAGG00000006506.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035194">
        <property type="term" value="P:regulatory ncRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18077">
        <property type="entry name" value="DEXXQc_HELZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000419">
        <property type="entry name" value="Probable helicase with zinc finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000453">
        <property type="entry name" value="Probable helicase with zinc finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049569">
        <property type="entry name" value="HELZ_DEAD-box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF365">
        <property type="entry name" value="HELICASE WITH ZINC FINGER DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase/UvrB N-terminal" evidence="2">
        <location>
            <begin position="176"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="3">
        <location>
            <begin position="296"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="4">
        <location>
            <begin position="377"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="786"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="922"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1146"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1406"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="651"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="699"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="806"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="829"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="922"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1454"/>
            <end position="1476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1476" mass="167667" checksum="2C9AB997575308D2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1U5</accession>
    <name>A0A452H1U5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">IRF tryptophan pentad repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H1U5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008527"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009814.1">
        <property type="protein sequence ID" value="ENSGAGP00000008527.1"/>
        <property type="gene ID" value="ENSGAGG00000006772.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00103">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000003">
        <property type="entry name" value="Interferon regulatory factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000093">
        <property type="entry name" value="Putative interferon regulatory factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019817">
        <property type="entry name" value="Interferon_reg_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001346">
        <property type="entry name" value="Interferon_reg_fact_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019471">
        <property type="entry name" value="Interferon_reg_factor-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949:SF10">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00605">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10401">
        <property type="entry name" value="IRF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00267">
        <property type="entry name" value="INTFRNREGFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00348">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01243">
        <property type="entry name" value="IRF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00601">
        <property type="entry name" value="IRF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51507">
        <property type="entry name" value="IRF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="IRF tryptophan pentad repeat" evidence="10">
        <location>
            <begin position="7"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="501" mass="56432" checksum="0E1FEDE6D67E9A88" modified="2019-05-08" version="1">MNAHPRRIRLKPWLIAQVNSNQYPGLQWVNPEQKLFCIPWRHATRHIPTQDDENTVFKAWAKETGKYNEGVDEPDPAKWKANLRCALNKSREFKLIYDGTKDTPMQPYKIYEVYEGHQPNGDSLAGDDHTCCPGEEEEELQKLTTLTIADPSQCVEPLPSYVWPKQEVPYPNSCGNGSFPPLGTQELLHPDVSAPLPPTAGAFAAGELPTQSPAGSMPLGGPGSLQNLPPGDVSLQPMEQFIPDLLISPHMLPLTDLELKFHYRGRQASSLTISNPHGCRLFHSSLEPTHEQVELFGPVTLDQVRFPSTEHIPNEKQRFYTHQLLDVMDRGLILELQGQDIYAVRLCQCKVFWTGPCAGEPAGPNPIERERKIKLFSLENFLNGLILFQKGQTSTPPPFEIFLCFGEEWPDQKPKEKKLITVQVVPVAARILLEMFSGELSWSADSIRLQISHPDLKDKMVEQFKELHQLWQNQQSLQAHPASNLEPGPGPWAMQASSMQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H206</accession>
    <name>A0A452H206_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H206"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008435"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009711.1">
        <property type="protein sequence ID" value="ENSGAGP00000008435.1"/>
        <property type="gene ID" value="ENSGAGG00000006707.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15230">
        <property type="entry name" value="7tmA_OR5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="69"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="325" mass="36774" checksum="FCDCDBB86EDF3302" modified="2019-05-08" version="1">MSIYPLNVHLLPVLGICFNACLTLLGSFENLRNQTIIDEFIFLGLTNQQDLQVVLFMVFLMFFVTTLVGNLGMIILISIDSRLHTPMYFFLCNLSLIDLGCSLSVAPKMMVNFLAERKTISYSGCTTQMFFFGFCVLADCYFLAVMAYDRYVAICNPLLYRVVMSQRVCVYLAGGSYAVAIFCSNTINHFFCDIPPILKLSCSSTDINELVLFALGIFAGLSTLIFILISYLYILSSILRIGSAEGRQKAFSTCTSHLTAITLFYGTMIFMYLRPTSSYRLDQDKLVSLFYTVMIPMLNPLIYSLRNKNVRFKTWEVNVPLTWPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3F6</accession>
    <name>A0A452H3F6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TBC1 domain family member 23</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H3F6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009080"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010421.1">
        <property type="protein sequence ID" value="ENSGAGP00000009080.1"/>
        <property type="gene ID" value="ENSGAGG00000007169.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990403">
        <property type="term" value="P:embryonic brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099041">
        <property type="term" value="P:vesicle tethering to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20788">
        <property type="entry name" value="TBC1D23_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000017">
        <property type="entry name" value="TBC1 domain family member 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000002">
        <property type="entry name" value="TBC1 domain family member 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039755">
        <property type="entry name" value="TBC1D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045799">
        <property type="entry name" value="TBC1D23_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13297:SF5">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13297">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19430">
        <property type="entry name" value="TBC1D23_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="6">
        <location>
            <begin position="38"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="7">
        <location>
            <begin position="328"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="455"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="455"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="695" mass="78233" checksum="7CEE0521893FE0FA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4F7</accession>
    <name>A0A452H4F7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDENN FLCN/SMCR8-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SMCR8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H4F7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009367"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010767.1">
        <property type="protein sequence ID" value="ENSGAGP00000009367.1"/>
        <property type="gene ID" value="ENSGAGG00000007417.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032045">
        <property type="term" value="C:guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037521">
        <property type="entry name" value="FLCN/SMCR8_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037520">
        <property type="entry name" value="Folliculin/SMCR8_longin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31334:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE PROTEIN SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31334">
        <property type="entry name" value="SMITH-MAGENIS SYNDROME REGION GENE 8 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11704">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51834">
        <property type="entry name" value="DENN_FLCN_SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN FLCN/SMCR8-type" evidence="6">
        <location>
            <begin position="48"/>
            <end position="905"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="932" mass="105029" checksum="2EEAB6C704370640" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6M4</accession>
    <name>A0A452H6M4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RUN domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">May play a role as p53/TP53 inhibitor and thus may have oncogenic activity.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H6M4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010331"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011861.1">
        <property type="protein sequence ID" value="ENSGAGP00000010331.1"/>
        <property type="gene ID" value="ENSGAGG00000008099.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17683">
        <property type="entry name" value="RUN_RUNDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.900:FF:000012">
        <property type="entry name" value="RUN domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058732">
        <property type="entry name" value="RUNDC1_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47194:SF5">
        <property type="entry name" value="RUN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47194">
        <property type="entry name" value="SORTING NEXIN-29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26030">
        <property type="entry name" value="RUNDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="7">
        <location>
            <begin position="388"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="259"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00055063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="580" mass="63719" checksum="313343D1D82F9DA4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H758</accession>
    <name>A0A452H758_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tetratricopeptide repeat protein 7A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of a complex required to localize phosphatidylinositol 4-kinase (PI4K) to the plasma membrane. The complex acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns(4)P) synthesis. In the complex, plays a central role in bridging PI4KA to EFR3B and HYCC1, via direct interactions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H758"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010381"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011917.1">
        <property type="protein sequence ID" value="ENSGAGP00000010381.1"/>
        <property type="gene ID" value="ENSGAGG00000006479.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000066">
        <property type="entry name" value="Tetratricopeptide repeat domain 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000105">
        <property type="entry name" value="Tetratricopeptide repeat domain 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051722">
        <property type="entry name" value="Endocytosis_PI4K-reg_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045819">
        <property type="entry name" value="TTC7_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083:SF475">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN, TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19440">
        <property type="entry name" value="TTC7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 11" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Tetratricopeptide repeat protein 7 N-terminal" evidence="13">
        <location>
            <begin position="120"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="686"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="754"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF19440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="799" mass="89429" checksum="D3C6A2E614019DE9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7J4</accession>
    <name>A0A452H7J4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ankyrin repeat domain-containing protein 16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required to prevent the misactivation of serine (Ser) with tRNA(Ala) by promoting the hydrolysis of Ser-mischarged tRNA(Ala), thereby playing a role in translational fidelity. Binds directly to the catalytic domain of AARS/AlaRS and captures Ser that is misactivated by AARS/AlaRS, preventing the charging of Ser adenylates to tRNA(Ala) and precluding Ser misincorporation in nascent peptides.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with AARS; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H7J4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010508"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012068.1">
        <property type="protein sequence ID" value="ENSGAGP00000010508.1"/>
        <property type="gene ID" value="ENSGAGG00000008224.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000336">
        <property type="entry name" value="Ankyrin repeat domain-containing protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051616">
        <property type="entry name" value="Cul2-RING_E3_ligase_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46224">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46224:SF64">
        <property type="entry name" value="IQ MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="84"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="117"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="287"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="376" mass="41838" checksum="85AC583334AE1EF0" modified="2019-05-08" version="1">MSHSSSMAEAENQKQLLRLIQEGRLDLFKEELQRDEEMSKEVRRKHYGKSGDTLLHYAARHGHLNVLTHLVEALEMDIEMFNNDYKRPLHEAASMGHRDCVLYLLDKGATIDCLKKADWTPLMMACTRKNVEVIKDLIEHGANPLLKNKDGWNCFHIASREGDPQIIEYLLTVSPSSWNTESKIKRTPLHTAAMHGCFDVVKALLERCPYEPDSRDKCGVTPFMDAIQNGHIDIAQLLLEKHKACYTASDAVGAQPLHQAAVTAQDEAIRFLVSDLGANVNERATSVQLTALHYAAKEGHTSTIQMLLSLGADLHAKDGKSRSALHMACAGQHAACAWLLLQAGLQDSLDDTGTFARQLAKKPDVLQLFRGINIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7M4</accession>
    <name>A0A452H7M4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Afadin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Afadin adherens junction formation factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. May play a key role in the organization of epithelial structures of the embryonic ectoderm. Essential for the organization of adherens junctions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Adherens junction</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000010751"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012323.1">
        <property type="protein sequence ID" value="ENSGAGP00000010751.1"/>
        <property type="gene ID" value="ENSGAGG00000008373.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034330">
        <property type="term" value="P:cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22711">
        <property type="entry name" value="FHA_AFDN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15471">
        <property type="entry name" value="Myo5p-like_CBD_afadin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06789">
        <property type="entry name" value="PDZ_AFDN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01781">
        <property type="entry name" value="RA2_Afadin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000032">
        <property type="entry name" value="Afadin isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000033">
        <property type="entry name" value="afadin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000006">
        <property type="entry name" value="Afadin, adherens junction formation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000025">
        <property type="entry name" value="Afadin, adherens junction formation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028842">
        <property type="entry name" value="Afadin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037977">
        <property type="entry name" value="CBD_Afadin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10398">
        <property type="entry name" value="AFADIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10398:SF2">
        <property type="entry name" value="AFADIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ras-associating" evidence="13">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="13">
        <location>
            <begin position="205"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="14">
        <location>
            <begin position="642"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="12">
        <location>
            <begin position="988"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="86"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="513"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1088"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1216"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1288"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1556"/>
            <end position="1804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1094"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1113"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1133"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1238"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1294"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1351"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1375"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1392"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1437"/>
            <end position="1449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1455"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1476"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1502"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1565"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1606"/>
            <end position="1676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1693"/>
            <end position="1708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1709"/>
            <end position="1720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1743"/>
            <end position="1752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1760"/>
            <end position="1774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1819" mass="207423" checksum="38D7A9A4D4100E8B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H887</accession>
    <name>A0A452H887_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heterogeneous nuclear ribonucleoprotein L RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H887"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010762"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012334.1">
        <property type="protein sequence ID" value="ENSGAGP00000010762.1"/>
        <property type="gene ID" value="ENSGAGG00000008379.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12704">
        <property type="entry name" value="RRM4_hnRNPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000052">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein L like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055204">
        <property type="entry name" value="HNRNPL_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034817">
        <property type="entry name" value="hnRNPL_RRM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22976">
        <property type="entry name" value="RRM_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterogeneous nuclear ribonucleoprotein L RRM" evidence="1">
        <location>
            <begin position="79"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="175" mass="19461" checksum="DD6B494DCF904959" modified="2019-05-08" version="1">MKSKPGAAMVEMADGYAVDRAITHLNNNFMFGQKLNVCVSKQQAIMPGQSYGLEDGSCSYKDFSGSRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEDNFYEICDELGAKRPASVKVFSGKSERSSSGLLEWDSKSDALETLSFLNHYQMKNPNGPYPYTLKLCFSTAQHAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8D7</accession>
    <name>A0A452H8D7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H8D7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010991"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012586.1">
        <property type="protein sequence ID" value="ENSGAGP00000010991.1"/>
        <property type="gene ID" value="ENSGAGG00000008514.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004303">
        <property type="term" value="F:estradiol 17-beta-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047035">
        <property type="term" value="F:testosterone dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09805">
        <property type="entry name" value="type2_17beta_HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000074">
        <property type="entry name" value="Retinol dehydrogenase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313:SF3">
        <property type="entry name" value="17-BETA-HYDROXYSTEROID DEHYDROGENASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY 9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="415" mass="45842" checksum="0F0AF368A7B6C63D" modified="2019-05-08" version="1">MCHSNVRKCLAKNLSTELQRERMDTYISTADPSVLLCLGMTAAFGIITFYKAKKNQVEKRCISARGLLLLFLLEILCFVMLKSYIGLILFSMACLICNIYLSVQTMLPVDNKAVLITGSDSGFGHALAKYLDNLGFVVFAGVLNKNGSGAEELKRSCSHRLCVLQLDITSSAQIKEAYLKISEMVQNTGLWGVVNNAGILGFPADGELLPMSVYKQCMDVNFFGHVETTKMFLPLLRKSKGRLINISSMAGGIPMTRFAAYGASKAALSMFSGVMRQELAKWGIKVAAVHPAGFKTCIQGTSELWAKQEKHLLENLTPDVKKDYGEDYIEALKNFLLHMPVHSEPDLSPVLDDVLHALLAKSPHGLYTPGKSAYLTLCIFCYFPLWLYDFYIGKLQSNKSVPSALRASEAKNKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8W9</accession>
    <name>A0A452H8W9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Neuropeptide B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neuropeptide B/W family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H8W9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011009"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012607.1">
        <property type="protein sequence ID" value="ENSGAGP00000011009.1"/>
        <property type="gene ID" value="ENSGAGG00000008527.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000012611.1">
        <property type="protein sequence ID" value="ENSGAGP00000011013.1"/>
        <property type="gene ID" value="ENSGAGG00000008532.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007631">
        <property type="term" value="P:feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013298">
        <property type="entry name" value="Neuropept_B_pre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013297">
        <property type="entry name" value="Neuropept_BW_pre"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28553">
        <property type="entry name" value="NEUROPEPTIDE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28553:SF1">
        <property type="entry name" value="NEUROPEPTIDE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15180">
        <property type="entry name" value="NPBW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01888">
        <property type="entry name" value="NROPEPTIDEBW"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01889">
        <property type="entry name" value="PPNRPEPTIDEB"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604488" description="Neuropeptide B" evidence="7">
        <location>
            <begin position="25"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="13467" checksum="4B69BD6B07A87BEA" modified="2019-05-08" version="1">MQRASARALLCLALAALLGPPAQAWYRQAAGPSAYSVGRASGLLSGIRRAPHLRRAENWTDSAERRADPPRGASSSCNLRLLRPQALCVKDLALELPNCQLLSGAPSTIQCKADVTISLDPSDCNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H978</accession>
    <name>A0A452H978_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Arginine-hydroxylase NDUFAF5, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Putative methyltransferase NDUFAF5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H978"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011127"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012742.1">
        <property type="protein sequence ID" value="ENSGAGP00000011127.1"/>
        <property type="gene ID" value="ENSGAGG00000008600.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000199">
        <property type="entry name" value="arginine-hydroxylase NDUFAF5, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050602">
        <property type="entry name" value="Malonyl-ACP_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13090">
        <property type="entry name" value="ARGININE-HYDROXYLASE NDUFAF5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13090:SF1">
        <property type="entry name" value="ARGININE-HYDROXYLASE NDUFAF5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="11">
        <location>
            <begin position="33"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="281" mass="31517" checksum="CDFE9AA3F9FB739E" modified="2019-05-08" version="1">QPEPAKYDYLREEVGGRVADRVFDITRTFPLALDVGCGSGYIAQYLNKETVEKLFQVDMAENALKNTMETEIPTVSVVADEEFLPFKEDTFDLVVSSLSLHWVNDLPRAFREIHQVLKPDGVFIGAMFGGETLYELRCSLQLAEQEREGGFSPHISPFTDVTDLGHLLGRAGFNTLTVDTDEIQVNYPGMFEIMEDLKGMGESNCSWNRKPILHRETMLAAAAIYQEMYGSNDGSVPATFEIYYMIGWKFQESQARPAQRGSATVSFGDLAKINALISRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H996</accession>
    <name>A0A452H996_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H996"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011147"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012767.1">
        <property type="protein sequence ID" value="ENSGAGP00000011147.1"/>
        <property type="gene ID" value="ENSGAGG00000008626.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00098">
        <property type="entry name" value="IgC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051755">
        <property type="entry name" value="Ig-like_CS_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19971">
        <property type="entry name" value="SIGNAL-REGULATORY PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019275947" description="Ig-like domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="17"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="128"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="229"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="482"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="505" mass="54278" checksum="604D9F9F56916CF1" modified="2019-05-08" version="1">MGARVGLFSLLLGGVCAQLEVMVDSPVAAQLGSQALLPCTFTVANAPISPEYLAVHWYFQGQELASYDDSLSISRPGVSMDTEQLASGNASLVLHNVTVSEQGAYRCLVIHSPDRGEQSLYLAVLAVPTIAIPSKAVLRGEPNLLKCSVSGFHPADIAVTWLRAGEVLPASPLPAPQRNPDGTFNVSSSLTFQPTEQDQGVAFSCQVQHAALPRGALQTDFQLVFGVSPMVNIHAPTFVQGKAQVLMCDVKGFYPEAIAVNWLLNGVRTEAPRVNPNGTFNLESFYQFKPVAESEGAEISCEVQHETLSRPIVRSVRVHLEYEGTQVSVIVLLVSGTVSALAVAGVAFFLYKRSRAARQMFTASDIVGPKVWVPGEYVSLVCIGYHCSAGTEATWAKCQNEELMPIARGDPSGSDGEEQPLVPINSLYKVQTQMLPLDKGSATLATTLMFRVRSPEDLGARYQCCFISKDGRTLSTKEFGDYTLRPSAQTQGPAVDPAAARPAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HA40</accession>
    <name>A0A452HA40_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Immunoglobulin subtype domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HA40"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011583"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013262.1">
        <property type="protein sequence ID" value="ENSGAGP00000011583.1"/>
        <property type="gene ID" value="ENSGAGG00000008942.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="17483" checksum="6419325B7CAB63AA" modified="2019-05-08" version="1">PLCSRESTDPLLPGDCSPPGGRTRFSFMTSLRETTLGLSADYSRAAEDETELTGILGGSKFENGAWMVNTSSLVTVMAGRPPRVLVFDSSYKGGLRFSDESYSLQITDLRLEDTGSYSVEFNTDKEQFAYREFTLRVSSKCWGRGIPEGAWNPPRSGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAW8</accession>
    <name>A0A452HAW8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Keratin type II head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HAW8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011886"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013614.1">
        <property type="protein sequence ID" value="ENSGAGP00000011886.1"/>
        <property type="gene ID" value="ENSGAGG00000009164.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16208">
        <property type="entry name" value="Keratin_2_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Keratin type II head" evidence="1">
        <location>
            <begin position="20"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="124" mass="12964" checksum="AE224B7AE199EE51" modified="2019-05-08" version="1">MLPATMSRQSTVRVQSGKQGFSASSAIIPNSCRTSYSSCSVSRARGCGAGAGRVVGGGSCFGSRSLYNLGGSKRISMAGSSGGFLCAQWKCSTGRSKGLVQHYIGNLWHTWELSLALQSISPMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBY0</accession>
    <name>A0A452HBY0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Keratin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the avian keratin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HBY0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012283"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014067.1">
        <property type="protein sequence ID" value="ENSGAGP00000012283.1"/>
        <property type="gene ID" value="ENSGAGG00000009447.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003461">
        <property type="entry name" value="Keratin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31203">
        <property type="entry name" value="BETA-KERATIN-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31203:SF1">
        <property type="entry name" value="BETA-KERATIN-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02422">
        <property type="entry name" value="Keratin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0416">Keratin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="12283" checksum="D7F919AF27CAEBAC" modified="2019-05-08" version="1">MSCSSLCYPECGVTHPSPVSGSCNEPCVRQCPDSEVLIQPSPVVVTIPGPILSSFPQHSEVGAAGAPVVGPGLYGFGGRSGYGGLSGYGGGYGGLCVLPCIICIAKSLFLLTLTQIFTLTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBY8</accession>
    <name>A0A452HBY8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Keratin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the avian keratin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HBY8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012282"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014066.1">
        <property type="protein sequence ID" value="ENSGAGP00000012282.1"/>
        <property type="gene ID" value="ENSGAGG00000009446.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003461">
        <property type="entry name" value="Keratin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31203">
        <property type="entry name" value="BETA-KERATIN-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31203:SF1">
        <property type="entry name" value="BETA-KERATIN-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02422">
        <property type="entry name" value="Keratin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0416">Keratin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12051" checksum="79D5124EF68AE119" modified="2019-05-08" version="1">MSCSTLCYPECRAAPHSPVSGSCNEPCVRQCPDSKVLIRQSPFVTTLPGPILTFGAPVVRPAFEGSFGLGRLYGSEGCYRGLYGLGGLGGYWRGYGGLCRSLAHKGSFSLSPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HC30</accession>
    <name>A0A452HC30_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Forms paracellular channels: coassembles with CLDN19 into tight junction strands with cation-selective channels through the strands, conveying epithelial permeability in a process known as paracellular tight junction permeability. Involved in the maintenance of ion gradients along the nephron. In the thick ascending limb (TAL) of Henle's loop, facilitates sodium paracellular permeability from the interstitial compartment to the lumen, contributing to the lumen-positive transepithelial potential that drives paracellular magnesium and calcium reabsorption.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Cs(+)(in) = Cs(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78555"/>
            <dbReference type="ChEBI" id="CHEBI:49547"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Li(+)(in) = Li(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78551"/>
            <dbReference type="ChEBI" id="CHEBI:49713"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Mg(2+)(in) = Mg(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29827"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Rb(+)(in) = Rb(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78547"/>
            <dbReference type="ChEBI" id="CHEBI:49847"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Cell junction</location>
            <location evidence="19">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Cell membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 19">Belongs to the claudin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HC30"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012322"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014111.1">
        <property type="protein sequence ID" value="ENSGAGP00000012322.1"/>
        <property type="gene ID" value="ENSGAGG00000009470.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160184">
        <property type="term" value="P:paracellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000012">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003927">
        <property type="entry name" value="Claudin16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01447">
        <property type="entry name" value="CLAUDIN16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 19" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 19" id="KW-0796">Tight junction</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="237" mass="26414" checksum="0DA3420CB6C5B7DA" modified="2019-05-08" version="1">MRYLLQYVACFFAFFSAGFLIAATWTDCWMVNADDSLEVSTKCRGLWWECVTNVFDGIQTCDEYDSIFAEHPLKLVLTRAMMITADILAGFGFAFLLLGLDCVKFLMDEPTIKLRICLVSGVTLLIAGFPGLIGSVWYALEVYVERSSLVLHNVFLGIQYKFGWSCWLGMAGSLGCFLSGAVLTCCMYLFQDVGSGRFYSPNSLRKMYSPAGTTVANTYYPSSQTATAKMYAMDTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCF3</accession>
    <name>A0A452HCF3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HCF3"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014283.1">
        <property type="protein sequence ID" value="ENSGAGP00000012465.1"/>
        <property type="gene ID" value="ENSGAGG00000009575.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="119" mass="13019" checksum="8EFB1EF0211BBEF2" modified="2019-05-08" version="1">MSRGFSKNLTKAGSFNKDARICSKAKDHSWLSLRGLCTLLRSVCSGALSLFSVVWLPVLESAAEAGLLAGEVGLCSLLPTSSIFSKRLVTVAIAQYNNSSSGSPWNRLYKVFENWMNWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEL0</accession>
    <name>A0A452HEL0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">F-box/WD repeat-containing protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">F-box and WD-40 domain-containing protein 9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with SKP1 and CUL1.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HEL0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013255"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015175.1">
        <property type="protein sequence ID" value="ENSGAGP00000013255.1"/>
        <property type="gene ID" value="ENSGAGG00000010157.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000062">
        <property type="entry name" value="F-box/WD repeat-containing protein 9 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000641">
        <property type="entry name" value="F-box/WD repeat-containing protein 9 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855:SF34">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855">
        <property type="entry name" value="WD40 REPEAT PROTEIN 12, 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="12">
        <location>
            <begin position="82"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="175"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="228"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="464" mass="51702" checksum="BA567875AAC379A0" modified="2019-05-08" version="1">MDLALNASEDPQGSDNDSDAENKVDPDTQAQEYIDRVLSSAQPRHRAQYSPPKSLSVGPGAQASERRSPLELKKSPASDDVTSGLLALPLELILEICSYLEARFVLEVLPLVCRTLRDIVQDPVTWRIRLQKRISGCYPVVEEEDFDWPAACMELEEHLRHWACDDRRTEYFSLADGHFASIDSVLLLQGGALCVSGSRDRNVNLWDLRELGQSPDKVLVKTLGTERNGTHKGWVWSLASRDNKVCSGSWDSTVKLWDIEANGQQFGEISGKAPVLCLSYLPDILVTGTYDKKVTVYDPRATQAPLKSRKLHSSAVLSLMADEHFIISGSEDRTLVIFDRRGNSVLQRLQLESYLISMSYQGTQLWAGDNQGLLYVFENRGGRFQHIRYFDVGHRSQITGVWHSLGTLYTTSTDKTLRIHVPTDPPHTICSQTHNNVLNGISAEGNIVVAASGGPSLEIWRLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFC0</accession>
    <name>A0A452HFC0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aquaporin-5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient. Plays an important role in fluid secretion in salivary glands. Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells. Seems to play a redundant role in water transport in the eye, lung and in sweat glands.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer; each monomer provides an independent water pore. Interacts with TRPV4; the interaction is probably indirect and regulates TRPV4 activation by hypotonicity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFC0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013523"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015480.1">
        <property type="protein sequence ID" value="ENSGAGP00000013523.1"/>
        <property type="gene ID" value="ENSGAGG00000010332.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015670">
        <property type="term" value="P:carbon dioxide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000003">
        <property type="entry name" value="Lens fiber major intrinsic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023276">
        <property type="entry name" value="Aquaporin_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF38">
        <property type="entry name" value="AQUAPORIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02017">
        <property type="entry name" value="AQUAPORIN5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 16 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="271"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="277"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="301" mass="32646" checksum="D30498FE4A40330C" modified="2019-05-08" version="1">MCKEICTMAFLRAIMAEFLATMIFVFFGLSSALKWPSALPSILQISLAFGLAIGTLVQMFGHVSGAHINPAVTIAFLVGNHISFLRSLFYVVAQLVGAITGAGILYLVTPINTRGNLAVNALNNNTSPGQALVVEIILTFQLAMCIFASTDKRRTDAIGSPALSIGLSVTLGHLVGIYFTGCSMNPARSFAPAVVMKRFSTAHWVFWFGPIIGAILASLLYNYFLFPYSLNVSERVAIVKGTYEPEEEWEEQKEQRTKSMELTSPAQHLLGGAGNSIVPQPRSTRSQLPNWEVAQGARKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HH10</accession>
    <name>A0A452HH10_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gasdermin pore forming domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gasdermin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HH10"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014167"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016216.1">
        <property type="protein sequence ID" value="ENSGAGP00000014167.1"/>
        <property type="gene ID" value="ENSGAGG00000010784.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012501">
        <property type="term" value="P:programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040460">
        <property type="entry name" value="Gasdermin_pore"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042377">
        <property type="entry name" value="GSDME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15207">
        <property type="entry name" value="NONSYNDROMIC HEARING IMPAIRMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15207:SF2">
        <property type="entry name" value="PEJVAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04598">
        <property type="entry name" value="Gasdermin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gasdermin pore forming" evidence="4">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="354" mass="39910" checksum="3A77CE9BE819E70F" modified="2019-05-08" version="1">MFAAATKNFVKQVGDSGRLVPVPSLSEADKYQPLSLVIKKRKCFLSKKSKFASTPFTLKDILYGEKEISTGVSSYQLLNYEDKSDVSLYGRRGNQIMSDVGVNIAGSDSVAVKASFGIVTKHEVEVPTLLKELTTRKINFDHCLVRQSRESRKAVLCVVMESIRTTRQCSLSVHAGMRGETMRFHIIEDQNHKGRDKAIVFPAHTTIAFSVFELYIHLDGNFEFCVTPVSKGGFEKEQSGSFSLNKLRRNLFHRNKRVMDIIANSDAYLEDIFTDYYEKAASMTDISSSCLREGSHIRVNLLNNNIPKGPCALCGMGNSKRETVYGCLECSFNGQKYVRLHAVPCFDVWHRRMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HI46</accession>
    <name>A0A452HI46_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kelch domain-containing protein 8B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in pinching off the separated nuclei at the cleavage furrow and in cytokinesis. Required for mitotic integrity and maintenance of chromosomal stability. Protects cells against mitotic errors, centrosomal amplification, micronucleus formation and aneuploidy. Plays a key role of midbody function involving abscission of the daughter cells during cytokinesis and appropriate chromosomal and nuclear segregation into the daughter cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HI46"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014575"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016674.1">
        <property type="protein sequence ID" value="ENSGAGP00000014575.1"/>
        <property type="gene ID" value="ENSGAGG00000011074.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110070">
        <property type="term" value="C:cellularization cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045171">
        <property type="term" value="C:intercellular bridge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902410">
        <property type="term" value="P:mitotic cytokinetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140014">
        <property type="term" value="P:mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098813">
        <property type="term" value="P:nuclear chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051746">
        <property type="entry name" value="Kelch_domain_containing_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260:SF2">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="359" mass="38350" checksum="D9B67A8FAB9ECF21" modified="2019-05-08" version="1">MPVPAMAATTARSFFWEVFPPMRTCRVYCSPAYQDGHLFVVGGCGQAGLPLDTVEMLDVHSQKWTVLPPLPTPRAGAAAVAVGKQVLVMGGMDSRQSPLASVEVFNTDEGKWEKVAALAQPSMGISAIERDGFVYALGGMGADTSPQALARVYDPVTTRWQLLPSMPTPCYGASAFLQGNKIFLLGGRQGKLPVTAFEGFDLETRSWTRYPSVPSRRAFAGCAVADGCFFSLGGLQQPGPHNFYSRPHFVNTVEMFDPEQGAWSKQGRAVRMREKRADFVAGCLGGRVVVAGGLGNQSFPLGSVEGFSISRKKWELLPPMPTGRCSCSSYQAPSLLFVIGGVAQGPSGAVEALCLREGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJP7</accession>
    <name>A0A452HJP7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HJP7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015118"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017271.1">
        <property type="protein sequence ID" value="ENSGAGP00000015118.1"/>
        <property type="gene ID" value="ENSGAGG00000011411.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18912">
        <property type="entry name" value="bHLH_TS_bHLHa9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="4">
        <location>
            <begin position="71"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="149"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="248"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="262" mass="28894" checksum="C1E1019A6B7A1E1D" modified="2019-05-08" version="1">MPRPALGKGTARPQEPDGAREELEGVAVSGAHLLGARQELWAPQGGKATTCPSDPAAAKLRKRSRPVRSKARRIAANVRERKRILDYNQAFNALRLALKHDLNGKRLSKIATLRRAINRIASLSMSLHASPVQRCPCAHTECRTWYNGPSQEGSGKGSQSQLIHLPPEPSYPDVPSFQHCPPSPPYPRYSPEPPLQSHYGSPKKDSFIASPAYYSSGNYYLGVRAICQQTHMDNFRDSLPGPVPWQPGYSQGSGYQQSLPRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKW6</accession>
    <name>A0A452HKW6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Secretogranin-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Secretogranin III</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HKW6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015573"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017789.1">
        <property type="protein sequence ID" value="ENSGAGP00000015573.1"/>
        <property type="gene ID" value="ENSGAGG00000011750.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030667">
        <property type="term" value="C:secretory granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033366">
        <property type="term" value="P:protein localization to secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026197">
        <property type="entry name" value="SCG3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17388">
        <property type="entry name" value="SECRETOGRANIN III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17388:SF2">
        <property type="entry name" value="SECRETOGRANIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15467">
        <property type="entry name" value="SGIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="114"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="240"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="386"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="392"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="503" mass="56877" checksum="6DE563A640B80023" modified="2019-05-08" version="1">EPCLSLWGWRLGIPSGTERTGPAHQRHLGTASGTCGHKSVVWGSRAGQLRCGSLLAPLVTLDKGIYNRELSEERPLEEQIAEAGAGKIRKTVSTENKPEFRNYSFPDDLNLLKSVTGKERNEKERESIRSSLYDHQQPAPDDADSTKNRRLVDDYDSTKSGLDYKFQDDPDGLHQLDGTPLTAEDIVQKIATRIYEENDRGVFDKIVSKLLNLGLITESQAYTLEDEVAEVLQQLIANEAKDREKESEDLDYPPSRTDSDTEEKQEEKMTPNVADQVGVMNGEIDDTLDNTWPSSSVLERRNELPSEDNFEDLQYFPNFYGLLKSLNSEEAKEKETLITIMKTLVDFVKMMVKYGTITPEEGVLYLENLDAMIALQTKSKLESPLTSTKNKLLADKSNEEADSTKEEAAKMEKQYETLKDSTKVKEQNTAGNNEEPKGKAEAYLEAIRKNIEWLKKHNKQGNKEDYDLSKLRDFIDQQADAYVDKGILDKEEADVIKRIYGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HL44</accession>
    <name>A0A452HL44_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coiled-coil domain containing 182</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HL44"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017883.1">
        <property type="protein sequence ID" value="ENSGAGP00000015655.1"/>
        <property type="gene ID" value="ENSGAGG00000011813.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031678">
        <property type="entry name" value="DUF4715"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37364">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37364:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15835">
        <property type="entry name" value="DUF4715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="17566" checksum="D765BFF918022418" modified="2025-10-08" version="2">MEPMYDTATIPMTLDMLTSKKVETNNPQARCLLSTLASAMCSQHASDVEKLQQELKNMQRDMKDFKQEVTRVICKIEGTLSYMMEVVTRLETRSNCVEQQLREEEDRGIVRNKVLTFLLPREKELREKCADLERRELGRGREFLFTPVFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQ79</accession>
    <name>A0A452HQ79_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Probable JmjC domain-containing histone demethylation protein 2C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Jumonji domain-containing protein 1C</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Probable histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May be involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the JHDM2 histone demethylase family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000017186"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019612.1">
        <property type="protein sequence ID" value="ENSGAGP00000017186.1"/>
        <property type="gene ID" value="ENSGAGG00000012825.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000008">
        <property type="entry name" value="Probable JmjC domain-containing histone demethylation protein 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054294">
        <property type="entry name" value="DUF7030"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054503">
        <property type="entry name" value="KDM3AB_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054504">
        <property type="entry name" value="PWWP_KDM3B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF6">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN 2C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22989">
        <property type="entry name" value="DUF7030"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22988">
        <property type="entry name" value="PWWP_KDM3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22987">
        <property type="entry name" value="Tudor_KDM3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjC" evidence="21">
        <location>
            <begin position="2273"/>
            <end position="2497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="254"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="610"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="922"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1044"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1144"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1216"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1325"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1450"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1608"/>
            <end position="1694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1968"/>
            <end position="2064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="297"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="351"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="387"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="625"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="660"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1048"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1159"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1221"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="1641"/>
            <end position="1650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1651"/>
            <end position="1667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1683"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1968"/>
            <end position="1991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="2015"/>
            <end position="2042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="2043"/>
            <end position="2064"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00056982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00079980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2539" mass="284012" checksum="843AC478943EFE92" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HR49</accession>
    <name>A0A452HR49_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HR49"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017524"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019984.1">
        <property type="protein sequence ID" value="ENSGAGP00000017524.1"/>
        <property type="gene ID" value="ENSGAGG00000012986.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002438">
        <property type="entry name" value="Neutral_aa_SLC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF109">
        <property type="entry name" value="INACTIVE SODIUM-DEPENDENT NEUTRAL AMINO ACID TRANSPORTER B(0)AT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01206">
        <property type="entry name" value="ORPHTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 9" id="KW-0769">Symport</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="95"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="472"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="556"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="598" mass="67358" checksum="A66441F9FD683826" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HR57</accession>
    <name>A0A452HR57_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane BAX inhibitor motif containing 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HR57"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019958.1">
        <property type="protein sequence ID" value="ENSGAGP00000017501.1"/>
        <property type="gene ID" value="ENSGAGG00000012999.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001234">
        <property type="term" value="P:negative regulation of apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF35">
        <property type="entry name" value="PROTEIN LIFEGUARD 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="12782" checksum="E6D39ACF2230CD5C" modified="2019-05-08" version="1">MSYPNAPPPYDDKNPLYPPQPGGYPQPGAYPGGYPHPGVPVPPMSTVPMNLGDGGSGEEDGLRAGTWDDKSVRHTFIRKVYTIISVQLLVTVGIIAVFTFVSPVRTFVQRNVAVYYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRV9</accession>
    <name>A0A452HRV9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[histone H3]-lysine(4) N-trimethyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.354</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60260"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.354"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.354" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A452HRV9"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020318.1">
        <property type="protein sequence ID" value="ENSGAGP00000017807.1"/>
        <property type="gene ID" value="ENSGAGG00000013220.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140999">
        <property type="term" value="F:histone H3K4 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF288">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="9">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="138" mass="16043" checksum="574E6B5DE08EF8C2" modified="2019-05-08" version="1">DRFVNSIWNERLLRCSQCKIARYCDTRCQKEAWQEHKRECKCLKSIEPNFPPDSVRLVGRIVFKLLGQSAACPSEKLYSFSDLQSNIEELSEEMKEGLKHLAQTLQLYLRVEIQDAFQLLPAIDIFQIFAKVSVKTDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSP7</accession>
    <name>A0A452HSP7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000018107"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020644.1">
        <property type="protein sequence ID" value="ENSGAGP00000018107.1"/>
        <property type="gene ID" value="ENSGAGG00000013415.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026523">
        <property type="entry name" value="PNMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048270">
        <property type="entry name" value="PNMA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095:SF17">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN MA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14893">
        <property type="entry name" value="PNMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="289"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="378" mass="42371" checksum="B6F1D98C7FF653A8" modified="2019-05-08" version="1">LCTHQQLLVLPSNPSSPLPPHLSTLALGHILEKFVLAYATSNSCRKLRLFAGEEEFEPWLEHTNEMLWKWAVPDVEKRRCLIESLSGPALDVIRTLKLIDPGVSMKDCLEALDNTFGSVEGPEDSYCKFLNSQQQRGEKISVYIQRLERLLQRGVMRGAVTAEQMDQPRLAQVVRGTQYQNPILLHLRLKERQEHPPSYSQLIKEVREEEERQAASECWEAQTLEPASTTPLPMASVLMVSTTEELAQQMQVLTERIAELQSIIDQAKISRNKAPQTVTREKGKGQFFCYRCGQDGHSAANCHNEENPSLVTEQEVLVMSTRLKGSKGALAMTEQLAEGELPEGVLVPSGVITLPAEAQEKVTILIXRSRLTRLPSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTZ0</accession>
    <name>A0A452HTZ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HTZ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018573"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021175.1">
        <property type="protein sequence ID" value="ENSGAGP00000018573.1"/>
        <property type="gene ID" value="ENSGAGG00000013743.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15222">
        <property type="entry name" value="7tmA_OR51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000002">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF104">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="43"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="314" mass="35402" checksum="EF2F1BB98979FDE7" modified="2019-05-08" version="1">AGPFTVTCFHPATFQLTGFPGQEAVCHWISIAFCVVYITAMVGNCTVLCIIWADRRLHQPMYLLLCMLSVNDLGMSLSTLPTVLSIFCFDVIDVTFDACLAQMFFIHFFSFMESGILLAMSFDRFVAIRDPLRYATILTNPRIVKIGLALVIKSTSMLLPFPFLIKRLPICKGNVLSHTYCLHADIMRLACADTTVNNIYGLFTILITYGLDSVFIILSYVKVLRTVFNIASHEQRLTALNTCASHICAVLLFYVPIIAVSVVHRFGGNAPRVVHILMSYAYLFVPPVLHPIVYSMKTKEIRKRISRIFSRDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HV27</accession>
    <name>A0A452HV27_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc finger CCHC domain-containing protein 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">May down-regulate transcription mediated by NF-kappa-B and the serum response element.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HV27"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018938"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021592.1">
        <property type="protein sequence ID" value="ENSGAGP00000018936.1"/>
        <property type="gene ID" value="ENSGAGG00000014018.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000021594.1">
        <property type="protein sequence ID" value="ENSGAGP00000018938.1"/>
        <property type="gene ID" value="ENSGAGG00000014018.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000017">
        <property type="entry name" value="zinc finger CCHC domain-containing protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000091">
        <property type="entry name" value="Zinc finger CCHC-type-containing 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042246">
        <property type="entry name" value="ZCCHC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46242:SF1">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46242">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 9 ZCCHC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="12">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="12">
        <location>
            <begin position="180"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="240" mass="27071" checksum="0F24060DD2D11E28" modified="2019-05-08" version="1">MTRWARTSTTHSKKPLDATPWEDMKNGSTSGTVRNKQQNYSNTLSLKNGQVKKKKNKNKKDYLNEDVNGFMEYLKQSSQIIHNGKVRAADSHEVREEVATALKKDRRREGRRLKRQEMKKNAMVCFHCKKPGHGIADCPAALESQDMGTGICYRCGSTEHEITKCRAKVDPALGEFPYAKCFICGEMGHLSRSCPDNPKGLYAEGGCCRLCGSVEHFKRDCPENQNSGEIQRNIACMSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVU6</accession>
    <name>A0A452HVU6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Somatoliberin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Growth hormone-releasing factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Growth hormone-releasing hormone</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glucagon family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HVU6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019273"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021955.1">
        <property type="protein sequence ID" value="ENSGAGP00000019273.1"/>
        <property type="gene ID" value="ENSGAGG00000014240.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016608">
        <property type="term" value="F:growth hormone-releasing hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031770">
        <property type="term" value="F:growth hormone-releasing hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051428">
        <property type="term" value="F:peptide hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030252">
        <property type="term" value="P:growth hormone secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000532">
        <property type="entry name" value="Glucagon_GIP_secretin_VIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046963">
        <property type="entry name" value="VIP/GHRH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11213">
        <property type="entry name" value="GLUCAGON-FAMILY NEUROPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11213:SF6">
        <property type="entry name" value="SOMATOLIBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00123">
        <property type="entry name" value="Hormone_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00070">
        <property type="entry name" value="GLUCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019432038" description="Somatoliberin" evidence="8">
        <location>
            <begin position="17"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Glucagon / GIP / secretin / VIP family" evidence="9">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="174" mass="20041" checksum="2D54E4B787DE918B" modified="2019-05-08" version="1">MLHRAIFLLFLHLVMCSISSPLYPALRYNSLPISSKTVSFQLQDGSPVQSNNLSVEEQQQEEEGFLANPAEKRMQRHADAIFTDHYRKVLGQISARKILQTIIGKRLGERSPEEGEHEFLTRRQSESILMDSRYRQQMVLKNFLGAILQNQRPQDANTDHVEDFTNTLANLMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVX9</accession>
    <name>A0A452HVX9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing], mitochondrial</fullName>
            <ecNumber evidence="4">1.3.1.124</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">2,4-dienoyl-CoA reductase [NADPH]</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in mitochondria. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2E,4E)-hexadienoyl-CoA + NADPH + H(+) = (3E)-hexenoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:84788"/>
            <dbReference type="ChEBI" id="CHEBI:84790"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2E,4E)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:45912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85101"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a (2E,4Z)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:61892"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85099"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.124" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452HVX9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019306"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021990.1">
        <property type="protein sequence ID" value="ENSGAGP00000019306.1"/>
        <property type="gene ID" value="ENSGAGG00000014256.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008670">
        <property type="term" value="F:2,4-dienoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05369">
        <property type="entry name" value="TER_DECR_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000288">
        <property type="entry name" value="2,4-dienoyl-CoA reductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43658:SF14">
        <property type="entry name" value="2,4-DIENOYL-COA REDUCTASE [(3E)-ENOYL-COA-PRODUCING], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43658">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="308" mass="32971" checksum="BF5352F67331C87F" modified="2019-05-08" version="1">MLPPNAFQGKVAFITGGGTGLGKGMTTALSSLGAECVISSRKIDVLKQTAEEISSKTGNKVHAIQCDVRDPTSVKNAVAELIKVAGHPDVVINNAAGNFVSPTERLSANAWKTIVDIVLNGTAFVTLEIGKELIKVQKGAAFLAITTIYAESGSGFVSPSASAKAGVDAMCKSLAAEWGKYGMRFNVIQPGPIRTKGAFSRIDPTGTFEKEMIGRIPCGRLGTVEEIANLATYLCSEYASWVTGAVIRLDGGEYVSMAGEFNDLRKVVSYMHTIYIQTYLKIYIKLYTDSSEMLFLLILCRLPMSSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWJ0</accession>
    <name>A0A452HWJ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NHR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HWJ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019546"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022255.1">
        <property type="protein sequence ID" value="ENSGAGP00000019546.1"/>
        <property type="gene ID" value="ENSGAGG00000014375.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12887">
        <property type="entry name" value="SPRY_NHR_like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000001">
        <property type="entry name" value="neuralized-like protein 4 isoform X1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037962">
        <property type="entry name" value="Neuralized"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006573">
        <property type="entry name" value="NHR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429">
        <property type="entry name" value="NEURALIZED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429:SF14">
        <property type="entry name" value="NEURALIZED-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07177">
        <property type="entry name" value="Neuralized"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00588">
        <property type="entry name" value="NEUZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51065">
        <property type="entry name" value="NHR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NHR" evidence="2">
        <location>
            <begin position="49"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="NHR" evidence="2">
        <location>
            <begin position="300"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="NHR" evidence="2">
        <location>
            <begin position="500"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="NHR" evidence="2">
        <location>
            <begin position="696"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="674"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="684"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="864" mass="92632" checksum="61D287536F5C9B85" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXW2</accession>
    <name>A0A452HXW2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ras-related protein Rab-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Lipid-anchor</topology>
            <orientation evidence="1 11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HXW2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019990"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022774.1">
        <property type="protein sequence ID" value="ENSGAGP00000019990.1"/>
        <property type="gene ID" value="ENSGAGG00000014725.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01865">
        <property type="entry name" value="Rab3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000206">
        <property type="entry name" value="Ras-related protein Rab-3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037872">
        <property type="entry name" value="Rab3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0488">Methylation</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="6 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="227" mass="25937" checksum="B4433C0DD9D3D893" modified="2019-05-08" version="1">MRHEAPVQMASAQDSRYGQKDTSDQNFDYMFKLLIIGNSSVGKTSFLFRYADDSFTSAFVSTVGIDFKVKTVFKNEKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVILVGNKCDMEDERIISTERGKHLAEQLGFEFFETSAKDNINVKQTFERLVDIICDKMSESLETDPAIAAGKQNTRLKDTPPPQQPNCGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXY7</accession>
    <name>A0A452HXY7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">superoxide dismutase</fullName>
            <ecNumber evidence="2">1.15.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Nitrite reductase CYGB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Pseudoperoxidase CYGB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Superoxide dismutase CYGB</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:20697"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Fe(II)-heme b-[protein] + nitric oxide + O2 = Fe(III)-heme b-[protein] + nitrate</text>
            <dbReference type="Rhea" id="RHEA:78091"/>
            <dbReference type="Rhea" id="RHEA-COMP:18975"/>
            <dbReference type="Rhea" id="RHEA-COMP:18976"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:55376"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:78092"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Fe(III)-heme b-[protein] + nitric oxide + H2O = Fe(II)-heme b-[protein] + nitrite + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:77711"/>
            <dbReference type="Rhea" id="RHEA-COMP:18975"/>
            <dbReference type="Rhea" id="RHEA-COMP:18976"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:55376"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:77713"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>H2O2 + AH2 = A + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:30275"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:30276"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 15">Belongs to the globin family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452HXY7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020060"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022850.1">
        <property type="protein sequence ID" value="ENSGAGP00000020060.1"/>
        <property type="gene ID" value="ENSGAGG00000014783.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013314">
        <property type="entry name" value="Globin_lamprey/hagfish"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46783">
        <property type="entry name" value="CYTOGLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46783:SF3">
        <property type="entry name" value="GLOBIN FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01906">
        <property type="entry name" value="FISHGLOBIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 15" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Globin" evidence="16">
        <location>
            <begin position="2"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="151" mass="17119" checksum="D1D255E504D7F76C" modified="2019-05-08" version="1">MAFSEADVQRARGAWEKMYANAEDNGTTVLVRMFTEHPDTKSYFTHFKGMGTAEEMEQSDQVRSHGKRVLTTINDLVQHLDSTDAFLGIVNPLGKKHAVQLKVDPKNFRIICDIILQVMEEKYGGDCKASFEKVTNEICTRLNNAYKEAGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYG0</accession>
    <name>A0A452HYG0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rap guanine nucleotide exchange factor 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HYG0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020274"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023097.1">
        <property type="protein sequence ID" value="ENSGAGP00000020274.1"/>
        <property type="gene ID" value="ENSGAGG00000014822.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000023103.1">
        <property type="protein sequence ID" value="ENSGAGP00000020278.1"/>
        <property type="gene ID" value="ENSGAGG00000014822.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000002">
        <property type="entry name" value="Rap guanine nucleotide exchange factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.870.10:FF:000013">
        <property type="entry name" value="rap guanine nucleotide exchange factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF26">
        <property type="entry name" value="RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="4">
        <location>
            <begin position="98"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="3">
        <location>
            <begin position="375"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="611" mass="71408" checksum="A65792BE8D6CE620" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I005</accession>
    <name>A0A452I005_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IRG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I005"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020773"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023659.1">
        <property type="protein sequence ID" value="ENSGAGP00000020773.1"/>
        <property type="gene ID" value="ENSGAGG00000015288.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000541">
        <property type="entry name" value="Immunity related GTPase M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051515">
        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341:SF17">
        <property type="entry name" value="IRG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRG-type G" evidence="5">
        <location>
            <begin position="48"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="430" mass="48429" checksum="8A16B0F10CC14D46" modified="2019-05-08" version="1">CLLRAMPLIPGASEPPPKAAMAGRLSRRGNLMAAAAELQKKLESIENTPLDIAIMGESGAGKSSFVNAIRGLHDDDEGAAKTGVKQTTMKRKAYPYPRLQNVTIWDLPGIGTPSFEADKYLIWVKFNNYDFFIIVTATCFTSHHITLARAIHRLGKRFYYMRSKVDIDLRAEQRKRNFNKERTLQEIREDCIENLRKAGETSLWVFLLCSWELTEYDFQLLQETLENELDDRKRHIFILVMPNISAKILEKKKAELQEHIWEVALLSCAIGVLPVPGLSLICDVAILVFHMKRYCLAFGLDEESLTRLAKQIDKPVAELKSAIRNSPLASAITKEFILSLLSRSLCGALMVVELILNFVPGLGSLAGGGISFATTYYMLQSFLDDTAEDAQNILTKALEPRAEESIKQHQDRLYMPIENQTHLSQPCQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0N4</accession>
    <name>A0A452I0N4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452I0N4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021024"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023945.1">
        <property type="protein sequence ID" value="ENSGAGP00000021024.1"/>
        <property type="gene ID" value="ENSGAGG00000015440.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11928">
        <property type="entry name" value="SH3_SH3RF3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11931">
        <property type="entry name" value="SH3_SH3RF3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11925">
        <property type="entry name" value="SH3_SH3RF3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11785">
        <property type="entry name" value="SH3_SH3RF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000063">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase SH3RF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000091">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase SH3RF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000001">
        <property type="entry name" value="Sorbin and SH3 domain-containing protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035816">
        <property type="entry name" value="SH3RF1/SH3RF3_SH3_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035612">
        <property type="entry name" value="SH3RF3_SH3_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF62">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SH3RF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="34"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="96"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="303"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="663"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="372"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="440"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="567"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="456"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="722" mass="77803" checksum="05E684530C25F6BD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0Q7</accession>
    <name>A0A452I0Q7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I0Q7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021028"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023949.1">
        <property type="protein sequence ID" value="ENSGAGP00000021028.1"/>
        <property type="gene ID" value="ENSGAGG00000015444.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000357">
        <property type="entry name" value="Fc receptor like 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF164">
        <property type="entry name" value="MAM DOMAIN-CONTAINING GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="27"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="118"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="285" mass="29228" checksum="E97B737F80B63CDF" modified="2019-05-08" version="1">SPRDLLILVNSLPLLSAPWGRVPVSPPHLTISAAGSWAAIGDVVVIQCESPRGSAPILYQFHHEGATLGNRTVSSRGPGSLALNVTSERDSGTYSCEADNGMPSLTCPDSLSVPVSPPHLTISAAGSWAAIGDVVVIQCESPRGSAPILYQFHHEGATLGNRTVSSRGPGSLALNVTSERDSGTYSCEADNGMASGPQHSDPFHLSVLGMPSPHALLGIGGAGSLRPSAFSCLLHLVLVLALSGLCLPCSAVLTVSSYPQGHMAPSPAPAPWPEAASTRKLGPPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1R7</accession>
    <name>A0A452I1R7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Caspase-3</fullName>
            <ecNumber evidence="13">3.4.22.56</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.</text>
            <dbReference type="EC" id="3.4.22.56"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.56" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A452I1R7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021424"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024399.1">
        <property type="protein sequence ID" value="ENSGAGP00000021423.1"/>
        <property type="gene ID" value="ENSGAGG00000015731.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000024400.1">
        <property type="protein sequence ID" value="ENSGAGP00000021424.1"/>
        <property type="gene ID" value="ENSGAGG00000015731.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031264">
        <property type="term" value="C:death-inducing signaling complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097194">
        <property type="term" value="P:execution phase of apoptosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030216">
        <property type="term" value="P:keratinocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043525">
        <property type="term" value="P:positive regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00032">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1470:FF:000002">
        <property type="entry name" value="Caspase-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000001">
        <property type="entry name" value="Caspase-3 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033139">
        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016129">
        <property type="entry name" value="Caspase_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454">
        <property type="entry name" value="CASPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454:SF198">
        <property type="entry name" value="CASPASE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Caspase family p20" evidence="18">
        <location>
            <begin position="50"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="17">
        <location>
            <begin position="189"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="282" mass="31627" checksum="C152E7BA13352A53" modified="2019-05-08" version="1">MADVKDELQSGHDEADAKSLFGSQGKSLPVSKSMDSEIQPDESYKMDYPEIGICIIINNKNFLPATGMSFRSGTDADAAHIRDTFMSLGYKVNLHNDRTGKQMFDILQNVAKDDHSNRSSFVCVVLSHGEDGLIYGTDGPFELKILTGLFRGDRCKSLVGKPKLFFFQACRGTELDSGVETDSSSEENTCQKMPVEADFLYAYSTVPGYYSWRNSSEGSWFIQSLCLMLKQHAKKLELMQILTRVNRKVAQFSSYSNQPGFHGKKQIPCIVSMLTKELYFVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2T4</accession>
    <name>A0A452I2T4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I2T4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021816"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024851.1">
        <property type="protein sequence ID" value="ENSGAGP00000021816.1"/>
        <property type="gene ID" value="ENSGAGG00000015993.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000099">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708">
        <property type="entry name" value="TENASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708:SF12">
        <property type="entry name" value="TENASCIN N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="2" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="46"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="222"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="372"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="503"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="639"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="10">
        <location>
            <begin position="725"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="938"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="944"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="959" mass="107412" checksum="D8FC02B198AB5FAA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3H0</accession>
    <name>A0A452I3H0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3H0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022073"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025158.1">
        <property type="protein sequence ID" value="ENSGAGP00000022073.1"/>
        <property type="gene ID" value="ENSGAGG00000016207.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000966">
        <property type="entry name" value="ZFP90 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000069">
        <property type="entry name" value="Zinc finger protein 572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002604">
        <property type="entry name" value="Zinc finger protein 715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="74"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="236" mass="26636" checksum="1642CF2F2FA210F0" modified="2019-05-08" version="1">DGVKTMEYSLTCIPQIPLTSLFFFACTPDRFHLSHIYFGLFIPFSIPLSEISFLSGAESDLCLDSFCLPSGDGMVSENEEEKPQQEDAVQVEPRGTLSGRSKGNVSRSCALSENTKACETQQRPEENYSSHSDLLTRDRINLEERRYACHECGKSFSRSSHLLRHQRIHTGETPYTCSECGKSFSQSSALSAHWRIHTGEKLYTCSECGKSFKQSPSLIRHHKIHLRRLKCSLTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3I7</accession>
    <name>A0A452I3I7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">S-adenosylmethionine decarboxylase proenzyme</fullName>
            <ecNumber evidence="14">4.1.1.50</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for biosynthesis of the polyamines spermidine and spermine. Promotes maintenance and self-renewal of embryonic stem cells, by maintaining spermine levels.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>S-adenosyl-L-methionine + H(+) = S-adenosyl 3-(methylsulfanyl)propylamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="14">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the eukaryotic AdoMetDC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A452I3I7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022099"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025191.1">
        <property type="protein sequence ID" value="ENSGAGP00000022099.1"/>
        <property type="gene ID" value="ENSGAGG00000016232.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006597">
        <property type="term" value="P:spermine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.90.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine decarboxylase proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.90.10">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048283">
        <property type="entry name" value="AdoMetDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001985">
        <property type="entry name" value="S-AdoMet_decarboxylase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018166">
        <property type="entry name" value="S-AdoMet_deCO2ase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00535">
        <property type="entry name" value="SAM_DCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11570">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11570:SF0">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01536">
        <property type="entry name" value="SAM_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001355">
        <property type="entry name" value="S-AdenosylMet_decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01336">
        <property type="entry name" value="ADOMETDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 18" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="5 14" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 14" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11 14" id="KW-0704">Schiff base</keyword>
    <keyword evidence="7 14" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5042320785" description="S-adenosylmethionine decarboxylase beta chain" evidence="19">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042320786" description="S-adenosylmethionine decarboxylase alpha chain" evidence="19">
        <location>
            <begin position="75"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="15">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="15">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="15">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="15">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis" evidence="18">
        <location>
            <begin position="74"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="17">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR001355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="340" mass="39322" checksum="F265F2B69010FF43" modified="2019-05-08" version="1">MEENGAHFFEGTEKLLEVWFAREQPSPQEPHQSKGSGDLRTIPRFEWDKLLENVHCLIISVTKTDKQEAYVLSESSMFVSKRRFILKTCGTTLLLQALVPLLELAREYSGFDSIQSFFYSRKNFMKPSHQEYPHRNFQEEVEFLNEIFPNGAAYCMGRMNSDCWYLYTLDFPESRVTNQPDQTLEILMSELDPVVMDQFYMKDGVTANDVTRVSGIRDLIPGSVIDATMFNPCGYSMNGMKSDGTYWTIHITPEPEFSYVSFETNIGQTSYDELIRKVVDVFKPGKFVTTLFVNQSSKCRTVFSSAQKIEGFKRVDRQIAQFNDYNFVFTSFAKKQQQHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3J6</accession>
    <name>A0A452I3J6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BLOC-1-related complex subunit 6 C-terminal helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I3J6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022066"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025151.1">
        <property type="protein sequence ID" value="ENSGAGP00000022066.1"/>
        <property type="gene ID" value="ENSGAGG00000016203.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019314">
        <property type="entry name" value="BORCS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046465">
        <property type="entry name" value="BORCS6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13440">
        <property type="entry name" value="BLOC-1 RELATED COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13440:SF7">
        <property type="entry name" value="BLOC-1 RELATED COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10157">
        <property type="entry name" value="BORCS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BLOC-1-related complex subunit 6 C-terminal helix" evidence="2">
        <location>
            <begin position="221"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="324" mass="34261" checksum="40AE8977344E40F5" modified="2019-05-08" version="1">ALHSAHVTPGRAATRLNPAVAGRSRQPHWWIPRANQRERHLFPAPSERERNGNFPSPHRPKRGVWEPLPGAPSWILPIPPSSGACWAPVSGGAMRGAPRPGRWERLVLGACEHLPGRGLPAGPFCPGQRGAVGIAGSGSVTAPKGWAGSFPFSASLASRSLEALSLECGAGARPPGGRRATIATALEVEGSVRREGLLTHFVASNLQRKIQLSAPAASPIPPVDPAALRDLVALAGQVAAQVDELLRSVHCGLQALTALSVGCIQTYRDGVESLGEAVDTSIRAMYTLVARCEELDLAMQPVPALARRIRDMKGTLERLEGLCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3U1</accession>
    <name>A0A452I3U1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3U1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022186"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025290.1">
        <property type="protein sequence ID" value="ENSGAGP00000022186.1"/>
        <property type="gene ID" value="ENSGAGG00000016300.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033691">
        <property type="term" value="F:sialic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051036">
        <property type="entry name" value="SIGLEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12035">
        <property type="entry name" value="SIALIC ACID BINDING IMMUNOGLOBULIN-LIKE LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12035:SF125">
        <property type="entry name" value="SIALIC ACID-BINDING IG-LIKE LECTIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Immunoglobulin V-set" evidence="5">
        <location>
            <begin position="25"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="191" mass="20959" checksum="BB8E7396C19C2CC1" modified="2019-05-08" version="1">SPSVICKYYCQWFQLISSPGFTLKVPPSVSMQEGLCVLVPCTFAYPASYDTKNSLARLYRYWYKDPADVSHDSLVASSHPSRGVSQETQGRIQLMMNPANGDCSLQISDARRTDVGRYFFRVEKGSLKYNYLSNSDGTVAKLAISVPGKDLGRPAPGQTPVKWPVTSRCRSRAEGKSVAAFVSEGSNQASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I407</accession>
    <name>A0A452I407_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000022263"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025374.1">
        <property type="protein sequence ID" value="ENSGAGP00000022263.1"/>
        <property type="gene ID" value="ENSGAGG00000016352.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04980">
        <property type="entry name" value="IgV_L_kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="171" mass="18785" checksum="352ADCFABDDDBD23" modified="2025-10-08" version="2">MDVLVPTEIRTNASGQIVMTQTPESLAVSPEDRVTINCKASSSASTWYQQKSGQAPKLLIYYASTRATGIPDRFTGSGSGTDFTLTISNVQAEDAGDYYCQQYYSSPLTVIQTNTKTSLFXNLPVPANATHYLGTNFQPQKHIYLLSPPCNTYKLEDSLLLENLPSTQKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I708</accession>
    <name>A0A452I708_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="15">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A452I708"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023422"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026676.1">
        <property type="protein sequence ID" value="ENSGAGP00000023422.1"/>
        <property type="gene ID" value="ENSGAGG00000017162.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000020">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 15" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="11 15" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019319343" description="Pyruvate dehydrogenase E1 component subunit alpha" evidence="16">
        <location>
            <begin position="20"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="17">
        <location>
            <begin position="78"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="401" mass="44573" checksum="25BEE5B75DEB5EF2" modified="2019-05-08" version="1">MRKMLLAALSRVLQGPAAAARPGAVSEVSRVVVASRNYADFASEATFDIKKCDLHRLEEGPPVTAVLTREEGLHYYKTMQTIRRMELKSDQLYKQKIIRGFCHLYDGQEACCVGLEAAIKSTDHLITAYRAHGYAYTRGTSVREILAELTGRKGGCAKGKGGSMHMYAQNFYGGNGIVGAQVPLGAGIALACKYFGKDEICLTLYGDGAANQGQIFETYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDYIPGIRVDGMDILCVREATKFAAEHCRSGKGPILMELQTYRYHGHSMSDPGISYRTREEIQEVRSKSDPITLLKDRMLNNSLSSVEELKEIDVEVRKEIEEAAQFATTDPEPPLEELGNHIYKNEPPFEVRGPSQWIKYKSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I900</accession>
    <name>A0A452I900_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM6</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452I900"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024145"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027493.1">
        <property type="protein sequence ID" value="ENSGAGP00000024145.1"/>
        <property type="gene ID" value="ENSGAGG00000017661.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990518">
        <property type="term" value="F:single-stranded 3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902969">
        <property type="term" value="P:mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17757">
        <property type="entry name" value="MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000091">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000004">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000115">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008049">
        <property type="entry name" value="MCM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041024">
        <property type="entry name" value="Mcm6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF73">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18263">
        <property type="entry name" value="WHD_MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01662">
        <property type="entry name" value="MCMPROTEIN6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="343"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="659"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="663"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="699"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="820" mass="92829" checksum="A2B49740A1A32101" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IA01</accession>
    <name>A0A452IA01_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Calpain small subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000024352"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027719.1">
        <property type="protein sequence ID" value="ENSGAGP00000024352.1"/>
        <property type="gene ID" value="ENSGAGG00000017793.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110158">
        <property type="term" value="C:calpain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46735:SF3">
        <property type="entry name" value="CALPAIN SMALL SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46735">
        <property type="entry name" value="CALPAIN, SMALL SUBUNIT 1 A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="27438" checksum="D60DF2B995619995" modified="2019-05-08" version="1">MFLVKNFLGGGGGGGGGGGGFGGNIGNVIGGFLSGGGGGGRAGGGGGAGGAMGIIGGVINAISEAAAQYNPEPPPPRSHFSNVEANESEEIRQFRRLFTQLAGDDMEVSASELMNILNKVVTRHPDLKTDGFGIDTCRSMVAVMDRHDREAGLRGVQVFVGEHQEVAGRVVGGGGRLSRVAETRLYPAFGHGDRRGNERLWGSQPGGGLLESTGLQGAFPAGIDVCHXAHLLLRCLLGTQNSKRRSPAYLPEVPGVSGRGAAVNLGWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBT8</accession>
    <name>A0A452IBT8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted phosphoprotein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IBT8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024986"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028446.1">
        <property type="protein sequence ID" value="ENSGAGP00000024986.1"/>
        <property type="gene ID" value="ENSGAGG00000018273.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001649">
        <property type="term" value="P:osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045780">
        <property type="term" value="P:positive regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002038">
        <property type="entry name" value="Osteopontin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10607">
        <property type="entry name" value="OSTEOPONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10607:SF1">
        <property type="entry name" value="OSTEOPONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00865">
        <property type="entry name" value="Osteopontin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00216">
        <property type="entry name" value="OSTEOPONTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00017">
        <property type="entry name" value="OSTEO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019220124" description="Secreted phosphoprotein 1" evidence="2">
        <location>
            <begin position="17"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="35374" checksum="E9DA64AB33561C94" modified="2019-05-08" version="1">MKIAVLCLCLITISFALPVSKLKRRSISESSEENHDSRNHHSNRHHHQHVHTQSRMRPAQTQELLEPPQQDCFFSDESVEDTEQQSLPEPASVSNEDDGDHDDNDSNDTDESDEDMVTGFPTDAPKTTPLTPALPTRGDNSGRGDSVAYRMRAKAKLAGVYHKKSSEFYKAAGKFIIHKVTEEDDSKPDTESQQVDSSEAQLAAHHFPRKSDISVELDDKSNMQDSNEVNNRPHDKSMENDSPQQLDCVEADSDNSKPYVTEDSHMSNESTEQQQAQTEDLQQVDGIPEVNDSNQTSESTEDTQDHNSIEDNEVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICT4</accession>
    <name>A0A452ICT4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ICT4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025636"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029170.1">
        <property type="protein sequence ID" value="ENSGAGP00000025636.1"/>
        <property type="gene ID" value="ENSGAGG00000018721.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000076">
        <property type="entry name" value="Leucine-rich repeat and Ig domain-containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000014">
        <property type="entry name" value="Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF12">
        <property type="entry name" value="KEKKON 5, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019198065" description="Ig-like domain-containing protein" evidence="12">
        <location>
            <begin position="41"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="556"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="410"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="619" mass="70510" checksum="84CB5520AB4277BF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFY7</accession>
    <name>A0A452IFY7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IFY7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026870"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030544.1">
        <property type="protein sequence ID" value="ENSGAGP00000026870.1"/>
        <property type="gene ID" value="ENSGAGG00000019563.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19792">
        <property type="entry name" value="Bbox2_TRIM62_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16608">
        <property type="entry name" value="RING-HC_TRIM62_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13744">
        <property type="entry name" value="SPRY_PRY_TRIM62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000389">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035830">
        <property type="entry name" value="PRY/SPRY_TRIM62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="11"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="88"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="277"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="125"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="475" mass="54327" checksum="DE475CF2F0D7233C" modified="2019-05-08" version="1">MACSLKDELLCSICLSIYQDPVSFGCEHYFCRKCITEHWVRQEQQGARDCPECRRTFAEPTLAPSLKLANIVERYAAFPLDAILSARRSSAPCKDHEKVKLFCLTDRAVVCFFCDEPAVHEQHQVTNVDDAFEELQRELKEQLQALQDSERGHAEALHLLRRQLAETKSSAKSLRATIGEAFERLHRLLRERQKAMLEELEADTARTLTDIEQKIQRYSQQLRKVQEGSQILQERLAEVDRHAFLAGIASLSERLKGKIHETNLTYEDFPTSKYMGPLQYTIWKSLFQDIHPVPAALTLDPVTAHQRLILSDDCTIVAYGNLHPQPLQDSPKRFDVEVSVLGSEAFGSGVHYWEVIVSEKTQWMIGLAHEAVTRKGSIQIQPSRGFYCIVMHDGNQYSACTEPWTRLNVKSKLEKVGVFLDYDKGLLIFYNADDMSWLYTFREKFPGKLCSYFSPGQSHANGKNVQPLRINTVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IK52</accession>
    <name>A0A452IK52_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Geranylgeranyl pyrophosphate synthase</fullName>
            <ecNumber evidence="8">2.5.1.1</ecNumber>
            <ecNumber evidence="7">2.5.1.10</ecNumber>
            <ecNumber evidence="6">2.5.1.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">(2E,6E)-farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Dimethylallyltranstransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Farnesyltranstransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Geranylgeranyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Geranyltranstransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>isopentenyl diphosphate + (2E)-geranyl diphosphate = (2E,6E)-farnesyl diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19361"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58057"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>isopentenyl diphosphate + dimethylallyl diphosphate = (2E)-geranyl diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22408"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:58057"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Isoprenoid biosynthesis; geranylgeranyl diphosphate biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homohexamer; trimer of homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 22">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.1" evidence="8"/>
    <dbReference type="EC" id="2.5.1.10" evidence="7"/>
    <dbReference type="EC" id="2.5.1.29" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452IK52"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028259"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032114.1">
        <property type="protein sequence ID" value="ENSGAGP00000028259.1"/>
        <property type="gene ID" value="ENSGAGG00000020534.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000032115.1">
        <property type="protein sequence ID" value="ENSGAGP00000028260.1"/>
        <property type="gene ID" value="ENSGAGG00000020534.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:(2E,6E)-farnesyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004161">
        <property type="term" value="F:dimethylallyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000009">
        <property type="entry name" value="Geranylgeranyl pyrophosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF44">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="22" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00062555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00072971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="300" mass="34733" checksum="E249C53E2BD2B53F" modified="2019-05-08" version="1">MEGMEETSERILLEPYKYLLQLPGKQVRTKLSQAFNHWLNVPEDKKQVIIEVTEMLHNASLLIDDIEDNSKLRRGFPVAHSIYGIPSVINSANYVYFLGLQKVLTLDHPEAVKVFTRQLLELHQGQGLDIYWRDTYTCPTEAEYKAMVLQKTGGLFGLAVGLMQLFSNYKRDLKPLLNTLGLFFQIRDDYANLHSKEYSENKSFCEDLTEGKFSFPTIHAIWSRPESTQVQNILRQRTENVDIKKYCVHYLENVGSFEYTRQTLQKLESEAYKQIEALGGNPELVALVEHLSKMFKENEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IM89</accession>
    <name>A0A452IM89_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">IRS-type PTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IM89"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029191"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033154.1">
        <property type="protein sequence ID" value="ENSGAGP00000029191.1"/>
        <property type="gene ID" value="ENSGAGG00000021116.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005104">
        <property type="term" value="F:fibroblast growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005068">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008543">
        <property type="term" value="P:fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01202">
        <property type="entry name" value="PTB_FRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050996">
        <property type="entry name" value="Docking_Protein_DOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038742">
        <property type="entry name" value="FRS2_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258">
        <property type="entry name" value="DOCKING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258:SF39">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR SUBSTRATE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00310">
        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRS-type PTB" evidence="7">
        <location>
            <begin position="13"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="312"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="436"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="476" mass="53754" checksum="9D91D9AFD76C8F05" modified="2019-05-08" version="1">MGSCYSYLCRDSVPDNHPTKFKVTNVDDEGNALGSGIMELTQMELILHTHKRDAVRWPYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCSRAEEIFNLLQDLMQCNSISVVEEPVVMTRGSHSTELELSRTPQAPNTLGYTVPGFPNGFHSFPGEAPSYSTARHPSMNSLRHSSIGEDSTHALIGLDEQSHTYVNTTSGEEEMRGRHCMHSLPEVHPPFLHGNHSCSLEDRNPQVFLQPGEVKFVLGPAPVHRHMVKRDSSCRYHRECGGHICAPNNNNNECKDECLVPRTPLLHYENLPSLPPVWECQPLRQDEDSGDALTPSPNGYPEPDEGDPLQNYMNSENTALHSGLRRDDFLQHRHNCKPSVFSFDFRRPCPEQQRQLNYIQVELEADPHKGRQSLQVPCTPLPAAHPASRMDSYAVIDLKKTAAMSNLQRALPRDDGTSRKTRHNSTDLPLPPCGHSCLSRRMELRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMH3</accession>
    <name>A0A452IMH3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RFamide neuropeptide family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IMH3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029052"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033004.1">
        <property type="protein sequence ID" value="ENSGAGP00000029052.1"/>
        <property type="gene ID" value="ENSGAGG00000021024.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031854">
        <property type="term" value="F:orexigenic neuropeptide QRFP receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024565">
        <property type="entry name" value="P518"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36476">
        <property type="entry name" value="OREXIGENIC NEUROPEPTIDE QRFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36476:SF1">
        <property type="entry name" value="OREXIGENIC NEUROPEPTIDE QRFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11109">
        <property type="entry name" value="RFamide_26RFa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0027">Amidation</keyword>
    <keyword evidence="5" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019479472" evidence="7">
        <location>
            <begin position="19"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="140" mass="15687" checksum="4C7D129A57CCADD4" modified="2019-05-08" version="1">MKAPYSLSCLLLLSLGSCVPPDDRRETGEPGDGLRFETSWQGRADDASQSGLWSEVLRRRRVEDLSSLFSVAKELQGFGKERAGFRFRFGRQESREEDGEGVGFLRGDGEKRSGTLGNLAEELNGYNRKKGGFSFRFGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQH9</accession>
    <name>A0A452IQH9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Golgi-associated PDZ and coiled-coil motif-containing protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">CFTR-associated ligand</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">PDZ protein interacting specifically with TC10</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IQH9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030363"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034459.1">
        <property type="protein sequence ID" value="ENSGAGP00000030363.1"/>
        <property type="gene ID" value="ENSGAGG00000021877.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030140">
        <property type="term" value="C:trans-Golgi network transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000009">
        <property type="term" value="P:negative regulation of protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06800">
        <property type="entry name" value="PDZ_GOPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000067">
        <property type="entry name" value="Golgi-associated PDZ and coiled-coil motif-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038879">
        <property type="entry name" value="GOPC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16528">
        <property type="entry name" value="GOLGI-ASSOCIATED PDZ AND COILED-COIL MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16528:SF2">
        <property type="entry name" value="GOLGI-ASSOCIATED PDZ AND COILED-COIL MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="259"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="372"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="55"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="126"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00083668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="429" mass="47588" checksum="8BFFD7BD0E6AA51E" modified="2019-05-08" version="1">MFRWLEVLEKEFDKAFVDVDLLLGEIDPDQADITYEGRQKMTSLSSCFAQLCHKAQTVSQINHKLEAQLVDLKSELTETQAEKVVLEKEVHDQLLQLHAIQLQLHAKTGQSVDSGAIKAKLSILSVDELERELEANKKEKVKEAQLEAEVKLLRKENEALRRHIAVLQAEVYGARLAAKYLDKELAGRVQQIQLLGRDMKGPAHDKLWNQLEAEIHLHRHKTVIRACRGRNDLKRPMQAPPGHEPDSLKKSQGVGPIRKVLLVKEDHEGLGISITGGKEHGVPILISEIHPGQPADRCGGLHVGDAILAVNGVNLRDAKHKEAVTILSQQRGEIEFEVVYVAPEVDSDDENVEYEDESGHRYRLYLDELEEGGNSGSNRKNASAAVKPSEVFVKKQSGVGHENGDLGNSVETPLEETAPKLADSAESSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQS5</accession>
    <name>A0A452IQS5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ODC antizyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IQS5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030320"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034413.1">
        <property type="protein sequence ID" value="ENSGAGP00000030320.1"/>
        <property type="gene ID" value="ENSGAGG00000021855.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008073">
        <property type="term" value="F:ornithine decarboxylase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075523">
        <property type="term" value="P:viral translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.60:FF:000001">
        <property type="entry name" value="Ornithine decarboxylase antizyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002993">
        <property type="entry name" value="ODC_AZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038581">
        <property type="entry name" value="ODC_AZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279:SF6">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02100">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01337">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0688">Ribosomal frameshifting</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="175" mass="19278" checksum="59DC5EF5F03549CA" modified="2019-05-08" version="1">VTVPSYHAPGTLFQGLCGAPDAPHPLSKIPGGRGGGRDPSLIYKDEKLTVNQDVSVHEGKPHIVHFQYKITEVKISSWDAVLSNQSLFVEIPDGLLADGSKEGLLALLEFAEEKMKANYVFICFRKSREDRAPLLKTFSFLGFEIVSPGHPCVPSRPDVMFMVYSLDQNSSSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRL5</accession>
    <name>A0A452IRL5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glucose-6-phosphatase 3</fullName>
            <ecNumber evidence="4">3.1.3.9</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glucose-6-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.9" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452IRL5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030502"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034610.1">
        <property type="protein sequence ID" value="ENSGAGP00000030502.1"/>
        <property type="gene ID" value="ENSGAGG00000021966.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004346">
        <property type="term" value="F:glucose-6-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000018">
        <property type="entry name" value="Glucose-6-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016275">
        <property type="entry name" value="Glucose-6-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12591">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12591:SF2">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000905">
        <property type="entry name" value="Glucose-6-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="15">
        <location>
            <begin position="95"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000905-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000905-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="387" mass="42625" checksum="A0DBACEB33348188" modified="2019-05-08" version="1">MQTGGGFKVVPEATASAALQLLCPVVGPGPSNALPCRFSLAAMDALYSRGVLFAEMLQTGPPWLERFWLSMTFLADPKCIFVVFFPLAYFLDRKVGVAVLWIGLVSEWLNVVAKWILFGERPFWWVYESGFSSKEKVLLRQFPVSCETGPGSPSGHCMITGAALWPIVTALTALASRHSTSRLVKLTPFLAYFLLLLAVGLSRIFILAHFPHQVLGGIVAGAALGWMLEACVPAERKLSFYLATSLALLLSAIGLYWSMVALGVDIDWSIHLATKWCLNPEWIHIETRPFSSLCRDTASVVGLGLATHSSFYTQLRHERPGWKQQAVGAGLALAALQGLNDVVQPENIVLWYGLNFLKYASFPWVVVALVPWLVRLLPLTQSRPHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ISS1</accession>
    <name>A0A452ISS1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ISS1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031069"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035225.1">
        <property type="protein sequence ID" value="ENSGAGP00000031069.1"/>
        <property type="gene ID" value="ENSGAGG00000022319.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18467">
        <property type="entry name" value="BACK_KLHL28_BTBD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18257">
        <property type="entry name" value="BTB_POZ_KLHL28_BTBD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000001">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF441">
        <property type="entry name" value="KELCH-LIKE PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="38"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="574" mass="64631" checksum="D066D0D7F37A7488" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IT35</accession>
    <name>A0A452IT35_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein 40</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IT35"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031191"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035375.1">
        <property type="protein sequence ID" value="ENSGAGP00000031191.1"/>
        <property type="gene ID" value="ENSGAGG00000022416.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000116">
        <property type="entry name" value="Leucine-rich repeat-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000193">
        <property type="entry name" value="Leucine-rich repeat-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000206">
        <property type="entry name" value="leucine-rich repeat-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000265">
        <property type="entry name" value="Leucine-rich repeat-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF261">
        <property type="entry name" value="VASORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="6">
        <location>
            <begin position="145"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00071450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="603" mass="67391" checksum="D2BD1B6C7D4599C9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUM8</accession>
    <name>A0A452IUM8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc finger and BTB domain-containing protein 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IUM8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031773"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036032.1">
        <property type="protein sequence ID" value="ENSGAGP00000031773.1"/>
        <property type="gene ID" value="ENSGAGG00000022823.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18202">
        <property type="entry name" value="BTB_POZ_ZBTB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000633">
        <property type="entry name" value="Zinc finger and BTB domain containing 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001553">
        <property type="entry name" value="Zinc finger and BTB domain containing 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001897">
        <property type="entry name" value="Zinc finger and BTB domain containing 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000002">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000971">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001072">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001086">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000070">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048060">
        <property type="entry name" value="ZBTB11_BTB_POZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF41">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="14">
        <location>
            <begin position="214"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="566"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="594"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="645"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="673"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="701"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="729"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="760"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="788"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="816"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="852"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="880"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="908"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="140"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1047" mass="118857" checksum="0219AC0D36D67CCF" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUT9</accession>
    <name>A0A452IUT9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TPD52 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IUT9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031640"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035888.1">
        <property type="protein sequence ID" value="ENSGAGP00000031640.1"/>
        <property type="gene ID" value="ENSGAGG00000022727.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001235">
        <property type="term" value="P:positive regulation of apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007327">
        <property type="entry name" value="TPD52"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19307">
        <property type="entry name" value="TUMOR PROTEIN D52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19307:SF8">
        <property type="entry name" value="TUMOR PROTEIN D53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04201">
        <property type="entry name" value="TPD52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="30"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="211" mass="23325" checksum="5B8119F4A0E73453" modified="2019-05-08" version="1">MEAQQAQGLLETQPLHETDEETVTDIDFTSMISEEEREELKIELAKLEDEISTLRQVLAAKEKHLAEIKQKLGINLMNELKQNFSKSWHDMQTTSAYKKTHETLSHAGQKATAAISNVGTAISKKFGDMRSHSIGYSIRHSISMPAMRNSPTFKSFEEKVETTVTSLKTKVGGTSHSGGSFEEVLSSTVHASTQGSITGTPLPENEEELQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IXH6</accession>
    <name>A0A452IXH6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IXH6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032770"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037139.1">
        <property type="protein sequence ID" value="ENSGAGP00000032770.1"/>
        <property type="gene ID" value="ENSGAGG00000023418.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000003">
        <property type="entry name" value="Iroquois-class homeobox protein IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003893">
        <property type="entry name" value="Iroquois_homeo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211:SF17">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00548">
        <property type="entry name" value="IRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="122"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="124"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="182"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="349"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="320"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="376"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="482" mass="51308" checksum="949708F4D34E144E" modified="2019-05-08" version="1">MCLAMSYPQGYLYQPSASLALYSCPAYSTSVISGPRTDELGRSSSGSAFSPYAGSTAFTAPSPGYNSHLQYGTDPAAAAAAAAFTSYVGSPYDHTPGMAGSLGYHPYAAPLGSYPYGDPAYRKNATRDATATLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWTPRNRSEDEEEEENIDLEKNDEDEPQKLEEKGHPEAPETGGEEQKAAPGCERLQEPHSPAGQEAEGGLSDSDCKASAEERLEGRPAPPKAAGPAALVQCPASRILQQAAGGEEHQYRPPSAAAAALHPGELHPIAPSSNSTSVIHSPQQAALAKPKLWSLAEIATSADKSKESSSEVSPGAGPTQGPPLAGSSTSHSPSRSPSSQCPFPNSAVLSRPLYYASPFYPGYTNYSTFGHLHSHAGTNPAAGPPGSHFNGLNQTILNRAETLAKETKMIRSQSQVDLCKDSPYELKKGMSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZK1</accession>
    <name>A0A452IZK1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-ribulosamine 3-kinase</fullName>
            <ecNumber evidence="2">2.7.1.172</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-(D-psicosyl)-L-lysyl-[protein] + ATP = N(6)-(3-O-phospho-D-psicosyl)-L-lysyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61392"/>
            <dbReference type="Rhea" id="RHEA-COMP:15796"/>
            <dbReference type="Rhea" id="RHEA-COMP:15797"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:144621"/>
            <dbReference type="ChEBI" id="CHEBI:144622"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:61393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(6)-D-ribulosyl-L-lysyl-[protein] + ATP = N(6)-(3-O-phospho-D-ribulosyl)-L-lysyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48432"/>
            <dbReference type="Rhea" id="RHEA-COMP:12103"/>
            <dbReference type="Rhea" id="RHEA-COMP:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:90418"/>
            <dbReference type="ChEBI" id="CHEBI:90420"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.172"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:48433"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the fructosamine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.172" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452IZK1"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038016.1">
        <property type="protein sequence ID" value="ENSGAGP00000033554.1"/>
        <property type="gene ID" value="ENSGAGG00000023901.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000038018.1">
        <property type="protein sequence ID" value="ENSGAGP00000033556.1"/>
        <property type="gene ID" value="ENSGAGG00000023901.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102193">
        <property type="term" value="F:protein-ribulosamine 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1200.10:FF:000003">
        <property type="entry name" value="fructosamine-3-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000264">
        <property type="entry name" value="Protein-ribulosamine 3-kinase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016477">
        <property type="entry name" value="Fructo-/Ketosamine-3-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12149">
        <property type="entry name" value="FRUCTOSAMINE 3 KINASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12149:SF10">
        <property type="entry name" value="KETOSAMINE-3-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03881">
        <property type="entry name" value="Fructosamin_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006221">
        <property type="entry name" value="Ketosamine-3-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604630" description="protein-ribulosamine 3-kinase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR006221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="348" mass="38677" checksum="AFCE4B1DCFDD8870" modified="2019-05-08" version="1">MPSDSKGSVSRLLAAGLLQLLCSARGPPALARVRSQGSSMEAALKRELGTSVLRPTGHSGGGCISQGQSYDTDAGRVYVKSNSKAEARRMFEGEMESLSAILQTQTVKVPKPIKVIDVPGGSTLFVMEHLEMRSLNRHSGKLGTQLADLHLHNQKLGEKLKKEVNTVGKSEGQTDIQFVDRFGFHTVTCCGYLPQVNDWQNDWVTFYARQRIQPQMDMVEKNSGDREARELWAQLQLKIPSLFCDMEIVPALLHGDLWGGNVAEDDSGPIIFDPASFYGHSEYELAIAGMFGGFSNSFYSAYHSKIPKAPGFEKRLKLYQLFHYMNHWNHFGSGYRGSSLNIMRNLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0N9</accession>
    <name>A0A452J0N9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J0N9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033925"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038419.1">
        <property type="protein sequence ID" value="ENSGAGP00000033925.1"/>
        <property type="gene ID" value="ENSGAGG00000024124.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15087">
        <property type="entry name" value="7tmA_NPBWR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000102">
        <property type="entry name" value="neuropeptides B/W receptor type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009150">
        <property type="entry name" value="Neuropept_B/W_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF18">
        <property type="entry name" value="NEUROPEPTIDES B_W RECEPTOR TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01855">
        <property type="entry name" value="NRPEPTIDEWR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="71"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="345" mass="39202" checksum="20E7AC3092BFDBCB" modified="2019-05-08" version="1">MKSEYSSLSLKAGMENNFRVSNLNTTCNDTSYGRYLDNGTRSNLTFHEQSPDFYVVLPVIYSVICAVGLTGNTAVIYVILKAPKMKTVTNMFILNLAIADDLFTLVLPINIAEHLLHYWPFGAILCKIILSIDHYNIFSSIYFLTVMSIDRYLVVLATVRSKRMPHRTYRAARIVSLCIWALVTIIVLPFTIFANVYVDGLEIKSCGLNFPKPERFWFKASRIYTLILGFAIPVSTICILYTMMLYKLRNMHLNSNAKALDKAKKKVTIMVFIVLTVCLFCWTPFHLATIVALTTDLAQTSLVIGISYFITSLSYANSCLNPFLYAFLDDSFRKSFRKMLECRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J0Z7</accession>
    <name>A0A452J0Z7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">heme oxygenase (biliverdin-producing)</fullName>
            <ecNumber evidence="2">1.14.14.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heme oxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.18" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452J0Z7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033989"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038489.1">
        <property type="protein sequence ID" value="ENSGAGP00000033989.1"/>
        <property type="gene ID" value="ENSGAGG00000024161.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004392">
        <property type="term" value="F:heme oxygenase (decyclizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042167">
        <property type="term" value="P:heme catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006788">
        <property type="term" value="P:heme oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19165">
        <property type="entry name" value="HemeO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.910.10:FF:000001">
        <property type="entry name" value="Heme oxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002051">
        <property type="entry name" value="Haem_Oase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016053">
        <property type="entry name" value="Haem_Oase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018207">
        <property type="entry name" value="Haem_oxygenase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10720">
        <property type="entry name" value="HEME OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10720:SF2">
        <property type="entry name" value="HEME OXYGENASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01126">
        <property type="entry name" value="Heme_oxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00088">
        <property type="entry name" value="HAEMOXYGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00593">
        <property type="entry name" value="HEME_OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="317" mass="36073" checksum="3AC9CD52DC26366D" modified="2019-05-08" version="1">MPSDVTEASEGVDEAENVRYEETEEDDNVSPTDLSEMLKEGTKESHDRAENTQFVKDFLKGRIKKELFKLATVALYYTYSALEEEMDCNKDHPAFAPLYFPLELHRKEALAKDMEYFYGENWKEKIQCSEATRCYVDRIHYIGQYEPELLVAHAYTRYMGDLSGGQILKKVAQRALKLPSTGEGIQFYVFDNISNAQQFKQFYRARINALDLDKKAKERIVEEANKAFEFNMQVFDELDKIGALLTEEAQDGGLPVHDGKGDLRKCPYYATKLGKGDPGCPYHIGMAMLKQPTMQFVLAACIALAAGIATWYVMDGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1R7</accession>
    <name>A0A452J1R7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific protein-tyrosine kinase</fullName>
            <ecNumber evidence="1">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A452J1R7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034333"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038875.1">
        <property type="protein sequence ID" value="ENSGAGP00000034333.1"/>
        <property type="gene ID" value="ENSGAGG00000024399.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005131">
        <property type="term" value="F:growth hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007259">
        <property type="term" value="P:cell surface receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060397">
        <property type="term" value="P:growth hormone receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000114">
        <property type="entry name" value="Tyrosine-protein kinase JAK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051286">
        <property type="entry name" value="JAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45807">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE HOPSCOTCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45807:SF3">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE JAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="206" mass="24046" checksum="25E16D42EC77838C" modified="2019-05-08" version="1">MEYLPKGCLREYLQKNQERLDHKRLLLYAWQICKGMEYLGSQRYVHRDLASRNILVENETHVKIGDFGLAKLLPQDKEYYVVREPGQSPVFWYAPESLSDNVFSRESDVWSFGVVLYELFTYSNRSQSPSEEFLRMMGRDKPMPIICHLLELLKNDRRLPAPPGCPAEVHSLMLSCWAFSQSKRPKFSELGPKLEALRDSRSKVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1X5</accession>
    <name>A0A452J1X5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="20">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 20">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts (via UBP-type domain) with H2A; the interaction is less efficient than with monoubiquitinated H2A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the peptidase C19 family. USP3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A452J1X5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034387"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038930.1">
        <property type="protein sequence ID" value="ENSGAGP00000034387.1"/>
        <property type="gene ID" value="ENSGAGG00000024435.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000234">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000019">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF19">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="13" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 20" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 20" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9 20" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="22">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="21">
        <location>
            <begin position="159"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="520" mass="59170" checksum="0A5DDAF243F2E5DA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4M5</accession>
    <name>A0A452J4M5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Draxin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Dorsal inhibitory axon guidance protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Dorsal repulsive axon guidance protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">DRAXIN</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Chemorepulsive axon guidance protein required for the development of spinal cord and forebrain commissures. Acts as a chemorepulsive guidance protein for commissural axons during development. Able to inhibit or repel neurite outgrowth from dorsal spinal cord.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the draxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J4M5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035401"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040080.1">
        <property type="protein sequence ID" value="ENSGAGP00000035401.1"/>
        <property type="gene ID" value="ENSGAGG00000025166.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021528">
        <property type="term" value="P:commissural neuron differentiation in spinal cord"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021516">
        <property type="term" value="P:dorsal spinal cord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030900">
        <property type="term" value="P:forebrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03060">
        <property type="entry name" value="Draxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029094">
        <property type="entry name" value="Draxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28610">
        <property type="entry name" value="DRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28610:SF1">
        <property type="entry name" value="DRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15550">
        <property type="entry name" value="Draxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019593216" description="Draxin" evidence="5">
        <location>
            <begin position="28"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="120"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="350" mass="39068" checksum="572FC0E7F57FAB59" modified="2019-05-08" version="1" precursor="true">MASSSCSLSPFFFFCVLVLSDICSVGSLDSGTQDKNIPGNNNNLQNEELWQHQPRAGHHRRHGGAKKDRAHAMTSRGQVSGEEALRLASGTPAGDDQGAIQGPAALKQQKDVFLGFEFPYPERENQSPGSERGKKQNREHRRHSRRERLKQHRGKGPDPGPSSLYKKPENFEGQFQNLQVEEFTGLAPTVLLFTTLETAASTEEPPALSAASLRPQARVRQDGDVMPTLDMALFDWTDYEDLKPEMWPSAKRKEKHHSKSPSSGNETMAAEGEPCDHHLDCLPGSCCDLREHLCKPHNRGLNNKCYDDCMCTQGLRCYAKFHRNRRVTRRKGRCVEPESASGDQGSFINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GEV6</accession>
    <name>A0A452GEV6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[tau protein] + ATP = O-phospho-L-seryl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12801"/>
            <dbReference type="Rhea" id="RHEA-COMP:13701"/>
            <dbReference type="Rhea" id="RHEA-COMP:13702"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[tau protein] + ATP = O-phospho-L-threonyl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53904"/>
            <dbReference type="Rhea" id="RHEA-COMP:13703"/>
            <dbReference type="Rhea" id="RHEA-COMP:13704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GEV6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000065"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000073.1">
        <property type="protein sequence ID" value="ENSGAGP00000000065.1"/>
        <property type="gene ID" value="ENSGAGG00000000037.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14340">
        <property type="entry name" value="UBA_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000064">
        <property type="entry name" value="BR serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048622">
        <property type="entry name" value="BRSK1_2-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF108">
        <property type="entry name" value="BR SERINE_THREONINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21122">
        <property type="entry name" value="KA1_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21115">
        <property type="entry name" value="UBA_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="297"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="412"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="648" mass="72948" checksum="886F4725FCB794B2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GF82</accession>
    <name>A0A452GF82_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RUN domain-containing protein 3A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the RUNDC3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GF82"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000156"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000178.1">
        <property type="protein sequence ID" value="ENSGAGP00000000156.1"/>
        <property type="gene ID" value="ENSGAGG00000000127.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010753">
        <property type="term" value="P:positive regulation of cGMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17699">
        <property type="entry name" value="RUN_RUNDC3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.900:FF:000005">
        <property type="entry name" value="RUN domain-containing protein 3A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047338">
        <property type="entry name" value="RUN_RUNDC3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047340">
        <property type="entry name" value="RUNDC3A_B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46251">
        <property type="entry name" value="RUN DOMAIN-CONTAINING 3 PROTEIN RUNDC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46251:SF4">
        <property type="entry name" value="RUN DOMAIN-CONTAINING PROTEIN 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="6">
        <location>
            <begin position="52"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="263"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="438" mass="49432" checksum="1EAFCA580050B561" modified="2019-05-08" version="1">MESSCIQSAMALGLSSKKASSRNIAVERKNLITVCRFSVKTLLEKYTTEPIDDSSEEFINFAAILEQILSHCFKGPVSWFSSDGQRGFWDYIRLACSKVPNNCVSSIENMENISTSRAKGRAWIRVALMEKRMSEYITTALRDTRTTRRFYDDGAIMLREESTVLTGMLIGLSAIDFSFCLKGEVMDGKTPVVIDYTPYLKFTQSYDYLSDEEDRRSMESSTSEESSPEHPYQLLVTDEDSWYNKWRKMEQKFRIVYAQKGYLEELVRLRESQLKDLEAENKRLQMRLEEVKVQNQLEKKELEGVILELQEQLTGLIPCENTQLSKEMAMPLANQWPSLGTLSGNESGSDAKLYRRHSFMSTDQLSAEISLSSDSQRLGEGKQEGEPWGPLGKDPTPSMLGLCGSLASLPSCRSLASLKSNECLVSDSNEASPTHSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GG97</accession>
    <name>A0A452GG97_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibrillar collagen NC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GG97"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000604"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000678.1">
        <property type="protein sequence ID" value="ENSGAGP00000000604.1"/>
        <property type="gene ID" value="ENSGAGG00000000507.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1000:FF:000006">
        <property type="entry name" value="collagen alpha-1(XXVII) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000885">
        <property type="entry name" value="Fib_collagen_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01410">
        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51461">
        <property type="entry name" value="NC1_FIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0176">Collagen</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Fibrillar collagen NC1" evidence="4">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="112" mass="12841" checksum="659433C38C542992" modified="2019-05-08" version="1">MNFLHLLSSEVVQHITIHCLNMSVWREGSAEKPSEKAVRFKAWNGQIFEAEGPFKPKVATDDCKIQDGHWHKTVLTFWTQDPNQLPIVNIYNLPPSEPGKQYHLEVGPVCFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGA3</accession>
    <name>A0A452GGA3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H1/H5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GGA3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000578"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000649.1">
        <property type="protein sequence ID" value="ENSGAGP00000000578.1"/>
        <property type="gene ID" value="ENSGAGG00000000468.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000075">
        <property type="entry name" value="Histone H1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="5">
        <location>
            <begin position="37"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="96"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="121"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="151"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="208" mass="21230" checksum="42C8D1E8B0B8E83F" modified="2019-05-08" version="1">MSETAPVAAPAVSAPDAKAATKKPKKTTGGSKARKPSGPSVTELITKAVSASKERKGVSLAALKKALAAGGYDVEKSNSRIKVGLKSLLSKGTLVQTKGTGASGSFKLSKKLADTKEKAPKKPATKPKKPAAKKPKKAAIAKKSPTKVKKPAAAAPKKAAKSPKNIKAAKPKKAAKSPAKAKAVKPKVAKPKLTKPKVAKVKKAAPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGA5</accession>
    <name>A0A452GGA5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GGA5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000610"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000685.1">
        <property type="protein sequence ID" value="ENSGAGP00000000610.1"/>
        <property type="gene ID" value="ENSGAGG00000000512.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="31"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="146" mass="16635" checksum="662DC1868A8BDEB9" modified="2019-05-08" version="1">GALPLAGPTAPVDLPAEILARFGVTAAVWAQRFQEWKYRENKPPRSQLFNLIHLARKWLRPKACSPEEILETLVMHQYMRGLPPDLCKWVCQNDPSTYDEMITLADVHGQLKGRTLRQRRGQRPLPTPTSQSQLRGQRQIPQSLGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGW0</accession>
    <name>A0A452GGW0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the immunoglobulin superfamily. TIM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GGW0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000827"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000935.1">
        <property type="protein sequence ID" value="ENSGAGP00000000827.1"/>
        <property type="gene ID" value="ENSGAGG00000000694.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043277">
        <property type="term" value="P:apoptotic cell clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060097">
        <property type="term" value="P:cytoskeletal rearrangement involved in phagocytosis, engulfment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000774">
        <property type="entry name" value="Hepatitis A virus cellular receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46608">
        <property type="entry name" value="T-CELL IMMUNOGLOBULIN AND MUCIN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46608:SF3">
        <property type="entry name" value="T-CELL IMMUNOGLOBULIN AND MUCIN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="8"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="131" mass="14865" checksum="35A732C79BBC6662" modified="2019-05-08" version="1">YHPWQNHPSQGVSDTIVRAKVGQSIKLPCTYSVSQERDLNVMCWGRGICPSSKCSSEIVRTDGQKVISKQSGRYQLKGPITRGDVSLTISNVDHKDRGDYCCRIEIPGWFNDIKRNLHLQIYRDKSGRINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJH8</accession>
    <name>A0A452GJH8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PDZ GRASP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GORASP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GJH8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001839"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002091.1">
        <property type="protein sequence ID" value="ENSGAGP00000001839.1"/>
        <property type="gene ID" value="ENSGAGG00000001471.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000002094.1">
        <property type="protein sequence ID" value="ENSGAGP00000001841.1"/>
        <property type="gene ID" value="ENSGAGG00000001471.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000026">
        <property type="entry name" value="Golgi reassembly stacking protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007583">
        <property type="entry name" value="GRASP55_65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024958">
        <property type="entry name" value="GRASP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893">
        <property type="entry name" value="GOLGI REASSEMBLY STACKING PROTEIN GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893:SF2">
        <property type="entry name" value="GOLGI REASSEMBLY-STACKING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04495">
        <property type="entry name" value="GRASP55_65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51865">
        <property type="entry name" value="PDZ_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ GRASP-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ GRASP-type" evidence="8">
        <location>
            <begin position="58"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="154"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR607583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="374" mass="40735" checksum="5AA754276E830892" modified="2019-05-08" version="1">MLKDLLKANAEKAVKLEVYNIKSMKVREVEVIPSNMWGGQGLLGASVRFCTFQGANEHVWHVLDVEPASPAALAGLQPHTDYIVGSDQILQESEDFFSLIESHEGKPLKLMVYNTETDSCRELFVTPNGAWGGEGSLGCGIGYGYLHRIPTQPVMPKKQQEEVKPPSPLPLQDRSPVPSTNGYVEAPLLAPNSQRDSSEEVMNLDESTGQEMNMYSMENALHPPPPPPVQRVMDPGFLDESGVSFPEIVDLTKRLNLPSSAPFNMSAAESLAGTESLIPNDGTTTYFEHAMASVPADLTMYAEGSVTQSPLDHLMPSLSPLPSFALPNDISSKATVRTDSDAPEIELFEALPSTNSLKLDSDEKTHELEEAVQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKA8</accession>
    <name>A0A452GKA8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GKA8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002345.1">
        <property type="protein sequence ID" value="ENSGAGP00000002059.1"/>
        <property type="gene ID" value="ENSGAGG00000001668.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF133">
        <property type="entry name" value="RIKEN CDNA 1700020N01 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="11"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="59" mass="6819" checksum="89BA9EFD29B7BF68" modified="2019-05-08" version="1">IVGLFLFQGPVTFEEVAVYFTREEWALLDPVQRALYRDVMQENYETVTSLGKKSCPLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GL77</accession>
    <name>A0A452GL77_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HBS1-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">GTPase component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. The Pelota-HBS1L complex recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the Pelota-HBS1L complex, also named Dom34-Hbs1 complex, composed of PELO and HBS1L. Interacts with the SKI complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GL77"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002523"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002886.1">
        <property type="protein sequence ID" value="ENSGAGP00000002523.1"/>
        <property type="gene ID" value="ENSGAGG00000002033.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000002897.1">
        <property type="protein sequence ID" value="ENSGAGP00000002533.1"/>
        <property type="gene ID" value="ENSGAGG00000002033.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16267">
        <property type="entry name" value="HBS1-like_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04093">
        <property type="entry name" value="HBS1_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000039">
        <property type="entry name" value="HBS1-like translational GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000035">
        <property type="entry name" value="HBS1-like translational GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000020">
        <property type="entry name" value="Translation elongation factor EF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000204">
        <property type="entry name" value="Translation elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015033">
        <property type="entry name" value="HBS1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037189">
        <property type="entry name" value="HBS1-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08938">
        <property type="entry name" value="HBS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109732">
        <property type="entry name" value="HBS1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Tr-type G" evidence="15">
        <location>
            <begin position="262"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="688" mass="75930" checksum="F632691A447F8099" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLA2</accession>
    <name>A0A452GLA2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GLA2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002566"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002936.1">
        <property type="protein sequence ID" value="ENSGAGP00000002566.1"/>
        <property type="gene ID" value="ENSGAGG00000002035.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000002944.1">
        <property type="protein sequence ID" value="ENSGAGP00000002574.1"/>
        <property type="gene ID" value="ENSGAGG00000002035.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000021">
        <property type="entry name" value="Leucine-rich repeat and calponin homology domain-containing protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000007">
        <property type="entry name" value="Leucine-rich repeat and calponin homology domain-containing protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF44">
        <property type="entry name" value="LEUCINE RICH REPEATS AND CALPONIN HOMOLOGY DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="846"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="720"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="580"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="629"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="691"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="580"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="644"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="695"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="709"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="870" mass="97401" checksum="9BEEDD247FB0109C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLG5</accession>
    <name>A0A452GLG5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IRG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GLG5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002631"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003009.1">
        <property type="protein sequence ID" value="ENSGAGP00000002631.1"/>
        <property type="gene ID" value="ENSGAGG00000002114.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000541">
        <property type="entry name" value="Immunity related GTPase M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051515">
        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341:SF10">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRG-type G" evidence="5">
        <location>
            <begin position="43"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="401" mass="44334" checksum="BB086983731E824F" modified="2019-05-08" version="1">MSVDPVVVENKSPTINEEFKAAVQDGNLKAVVSIAQQMRKSPRLFEIAVVGESGSGKSSFVNALLGLADDDIGAAEGGMVETTDWSIIYSHPEYPNVLLCDLPGIGAPNFQSVEHEITDTYLLGYDFFLIVTATCFRDTHASLAQKIQENAKKFYFVRSKVDQDLGAEKRKPNYSEATTLWQIREDCQAQLQKAGVNDPQVFLLSSWESDKHDFPLLKENLDKELRHAMLQKLLDISCRTLQEKKTAMQKTTRKPATLSSGPGAIPLPGLSLVADMAVLVDCMRDYCKDFGLNSDSLSALAELVGKQEEDLKAVIRSPLATEITIDLVLKLLEKYVGEGVTYLTYFAWSVPVVGSLFSAQVSFGTTVFMLDNFISNLAEDAQRILVKALEGEEKKSKQHHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMT3</accession>
    <name>A0A452GMT3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Neural cell adhesion molecule 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMT3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002962"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003395.1">
        <property type="protein sequence ID" value="ENSGAGP00000002962.1"/>
        <property type="gene ID" value="ENSGAGG00000001319.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05866">
        <property type="entry name" value="IgI_1_NCAM-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05870">
        <property type="entry name" value="IgI_NCAM-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000086">
        <property type="entry name" value="Neural cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000381">
        <property type="entry name" value="Neural cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000436">
        <property type="entry name" value="Neural cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000450">
        <property type="entry name" value="Neural cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000528">
        <property type="entry name" value="Neural cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000636">
        <property type="entry name" value="Neural cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009138">
        <property type="entry name" value="Neural_cell_adh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF231">
        <property type="entry name" value="NEURAL CELL ADHESION MOLECULE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01838">
        <property type="entry name" value="NCAMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="680"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="18"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="110"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="206"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="299"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="398"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="495"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="749"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="749"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="774"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="784"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00055494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="822" mass="91720" checksum="65737940782C3466" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMW7</accession>
    <name>A0A452GMW7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMW7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003178"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003658.1">
        <property type="protein sequence ID" value="ENSGAGP00000003178.1"/>
        <property type="gene ID" value="ENSGAGG00000002564.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008392">
        <property type="term" value="F:arachidonate epoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019373">
        <property type="term" value="P:epoxygenase P450 pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF153">
        <property type="entry name" value="CYTOCHROME P450 2G1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="65" mass="7420" checksum="A59D9F71576C5A2B" modified="2019-05-08" version="1">MADRSHMPYTDAVIHEVQRTANIVPIGVPHAVTRDTHFREYIIPKVSSQCFCERARSSAFSTIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQH4</accession>
    <name>A0A452GQH4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4371 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GQH4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004176"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004892.1">
        <property type="protein sequence ID" value="ENSGAGP00000004176.1"/>
        <property type="gene ID" value="ENSGAGG00000003451.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF35">
        <property type="entry name" value="AC-LIKE TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="2">
        <location>
            <begin position="82"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="590"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="657" mass="75173" checksum="FB2309814128BB77" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQY9</accession>
    <name>A0A452GQY9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphofructokinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQY9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004372"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005136.1">
        <property type="protein sequence ID" value="ENSGAGP00000004372.1"/>
        <property type="gene ID" value="ENSGAGG00000003632.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF59">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE, MUSCLE TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="132" mass="14282" checksum="DFA4B44C151B4913" modified="2019-05-08" version="1">MSQPPEKHERTRNLGSGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGAKVYFVHEGYQGLVDGGDNIQEATWERVSMMLQLVGPGMNPLAGCEREPVPWATIVARLYKGARDPTLTEFQWDLIPKGQIFKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRB3</accession>
    <name>A0A452GRB3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SERRATE/Ars2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARS2 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000004502"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005277.1">
        <property type="protein sequence ID" value="ENSGAGP00000004502.1"/>
        <property type="gene ID" value="ENSGAGG00000003723.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031053">
        <property type="term" value="P:primary miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039727">
        <property type="entry name" value="SE/Ars2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007042">
        <property type="entry name" value="SERRATE/Ars2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165">
        <property type="entry name" value="ARSENITE-RESISTANCE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165:SF0">
        <property type="entry name" value="SERRATE RNA EFFECTOR MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04959">
        <property type="entry name" value="ARS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SERRATE/Ars2 C-terminal" evidence="5">
        <location>
            <begin position="11"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="184" mass="20528" checksum="B5552F5373D5AA51" modified="2019-05-08" version="1">MPNRCGIIHVRGPMPPNRVSHGEVAEWQKTFEEKLAPLSTMRESLSDEEAQKMGKKDPEQEVEKFVTANTQELGKDKWLCPLSGKKFKGPEFVRKHIFNKHAEKIEEVKKEVSFFNSFLMDAKRPALPEIXGPGQGLTPGLPYPHQTPQGLMPYGQPRPPIMGYGAPPCSPQTAPLTPAPGFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTU4</accession>
    <name>A0A452GTU4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vomeronasal type-1 receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTU4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005467"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006363.1">
        <property type="protein sequence ID" value="ENSGAGP00000005467.1"/>
        <property type="gene ID" value="ENSGAGG00000004434.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016503">
        <property type="term" value="F:pheromone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004072">
        <property type="entry name" value="Vmron_rcpt_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24062">
        <property type="entry name" value="VOMERONASAL TYPE-1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03402">
        <property type="entry name" value="V1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="256"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="303" mass="33924" checksum="D6C63B7F194F612E" modified="2019-05-08" version="1">MLICSGVSVGSVLSRWCSVFLKIILSNLALSNLLVILTQGIPITLKNFRLQNTYNDLVCKITLYIYCVGRAMSICITSLLGCFQCILIVPSPCKWLHLRENLLKNLSSVMISFCHTVVYNFCCMVFPSHLLYLGNGSVSVTRDLFFLGIMTLSSCYLLSMFYQHGKQAKHLLGLHTKHVEIQAAKAVVALLIMYLLCFGLDSLFWVYTVCMYPVSLSLIDAVQARTGLPSKVCESEVSFSEIGCRSLMMCWRGFSWGRYVMASGILLFFLLGLSCSRSLLGNRLALSICFLNFPGEYCSFKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GX07</accession>
    <name>A0A452GX07_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Histone-lysine N-methyltransferase SETDB1</fullName>
            <ecNumber evidence="16">2.1.1.366</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">SET domain bifurcated 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(6),N(6)-dimethyl-L-lysyl(9)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60288"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="2.1.1.366"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:60289"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.366" evidence="16"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006635"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007712.1">
        <property type="protein sequence ID" value="ENSGAGP00000006635.1"/>
        <property type="gene ID" value="ENSGAGG00000005348.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140947">
        <property type="term" value="F:histone H3K9me2 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006346">
        <property type="term" value="P:DNA methylation-dependent constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070828">
        <property type="term" value="P:heterochromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01395">
        <property type="entry name" value="HMT_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10517">
        <property type="entry name" value="SET_SETDB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20382">
        <property type="entry name" value="Tudor_SETDB1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21181">
        <property type="entry name" value="Tudor_SETDB1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000017">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000020">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000034">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000037">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000054">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040880">
        <property type="entry name" value="DUF5604"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025796">
        <property type="entry name" value="Hist-Lys_N-MeTrfase_SETDB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047232">
        <property type="entry name" value="SETDB1/2-like_MBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051516">
        <property type="entry name" value="SETDB_methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041292">
        <property type="entry name" value="Tudor_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041291">
        <property type="entry name" value="TUDOR_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46024">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE EGGLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46024:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETDB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18300">
        <property type="entry name" value="DUF5604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18358">
        <property type="entry name" value="Tudor_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18359">
        <property type="entry name" value="Tudor_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00391">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51573">
        <property type="entry name" value="SAM_MT43_SUVAR39_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="13 20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Pre-SET" evidence="23">
        <location>
            <begin position="728"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="22">
        <location>
            <begin position="804"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="24">
        <location>
            <begin position="1277"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="99"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="470"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="536"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="873"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1011"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1105"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="27"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="183"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="485"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="536"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="887"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="938"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1011"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1037"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1122"/>
            <end position="1138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1293" mass="144448" checksum="FA3C660EC01FFE14" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXA1</accession>
    <name>A0A452GXA1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Programmed cell death 6-interacting protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Midbody</location>
            <location evidence="1">Midbody ring</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXA1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006764"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007852.1">
        <property type="protein sequence ID" value="ENSGAGP00000006764.1"/>
        <property type="gene ID" value="ENSGAGG00000005456.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090543">
        <property type="term" value="C:Flemming body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09240">
        <property type="entry name" value="BRO1_Alix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09235">
        <property type="entry name" value="V_Alix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.280:FF:000001">
        <property type="entry name" value="programmed cell death 6-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF39">
        <property type="entry name" value="PROGRAMMED CELL DEATH 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="5">
        <location>
            <begin position="3"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="714"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="551"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="720"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="737"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="755"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="767"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="802"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00074846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="812" mass="89399" checksum="47C97B74486D485E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXL8</accession>
    <name>A0A452GXL8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DOK family. Type A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXL8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006776"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007868.1">
        <property type="protein sequence ID" value="ENSGAGP00000006776.1"/>
        <property type="gene ID" value="ENSGAGG00000005472.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01203">
        <property type="entry name" value="PTB_DOK1_DOK2_DOK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050996">
        <property type="entry name" value="Docking_Protein_DOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037751">
        <property type="entry name" value="Dok1/2/3_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258:SF46">
        <property type="entry name" value="DOCKING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258">
        <property type="entry name" value="DOCKING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00310">
        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="IRS-type PTB" evidence="5">
        <location>
            <begin position="155"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="322"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="490"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="516"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="582" mass="64514" checksum="C89E86C02EDBCD9B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXM9</accession>
    <name>A0A452GXM9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000006787"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007879.1">
        <property type="protein sequence ID" value="ENSGAGP00000006787.1"/>
        <property type="gene ID" value="ENSGAGG00000005479.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043549">
        <property type="entry name" value="C2C4C/C2C4D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46291:SF1">
        <property type="entry name" value="C2 CALCIUM-DEPENDENT DOMAIN-CONTAINING PROTEIN 4D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46291">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="328"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="357"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="369" mass="39416" checksum="A84031189BE9711F" modified="2019-05-08" version="1">MFLSRPRATNRKPGPLPTCPNVLTPDQIPKFFIPPKLSTLYGRAPGGSPGSQQSPQGNGSLETHRALIRAADRRVAHGQSLHQDPVARAEGEAGQQLPPACSMPHLVNPGGFPLLPESPHTRRRESLFHSTCPPHRISLDLSPEPGARLLPPELRLWWPVSQSDALDSDTASSAGSSPFSSPVLGHPLACQAHWGRPLCSRALDAQAGGRASSLSAEEASSTEDSPSFPRRQTEPSWGAAARLAPPPFSHLDFICRPARVAQENTVALSQGGCLRLSSKYVPASWCLHVRLVSAEGLYPRPCDPQRXPSVLAAAWPCSCDRARRRSSAAPWSRGAGAPSSMRTSSSRAWRPTSCPPAASGSRRSTRAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYE4</accession>
    <name>A0A452GYE4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PROTOR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GYE4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007081"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008199.1">
        <property type="protein sequence ID" value="ENSGAGP00000007081.1"/>
        <property type="gene ID" value="ENSGAGG00000005691.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013745">
        <property type="entry name" value="Bit61/PRR5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32428:SF3">
        <property type="entry name" value="PROLINE-RICH PROTEIN 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32428">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT BIT61-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08539">
        <property type="entry name" value="HbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="337"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="366" mass="40588" checksum="BD88C3BCFC4B7D44" modified="2019-05-08" version="1">MTGGSTLSPPFAVPFQKMGSFRRPRPRFMSSPVLSDLPRFQAARQALQLSSNSAWNSVQTAVINVFKGGGLQNNELYTLNENIRRLLKSELGSFITDYFQNQLLAKGLLFIEEKVKLCEGEDRIDILSETWDHFFTETLPTLQAIFYPVQGQELTIRQIALLGFRDLVLLKVKLDEILPLVQTKLPASIVQMLLVLQGVHEPTGPSKGYILLEELVKQVVSPYLGLCEDHSFPGGTCALERRYSRSRPKANLLSYSSHSVTARQPSETALTPLTEQEGEAYLEKCGSIRRHTVANAHSDIQLLAMASMMHTGIVIEEPDNSDKCLLLQPSFSHRQCSSEPSIADGPEGLVATGRQEPTELNCTSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYX1</accession>
    <name>A0A452GYX1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GYX1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007311"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008452.1">
        <property type="protein sequence ID" value="ENSGAGP00000007311.1"/>
        <property type="gene ID" value="ENSGAGG00000005870.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000007">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="51"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="139"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="180"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="222"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="273"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="307" mass="33648" checksum="DDAD48DE1C568622" modified="2019-05-08" version="1">DSTHSKLSDEFSLIFPLLQDARKACADATLAQITANIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDARCFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMVYSHDNIICGITSVAFSKSGRLLLAGYDDFNCNVWDTLKADRAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZV8</accession>
    <name>A0A452GZV8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cathelicidin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZV8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008810.1">
        <property type="protein sequence ID" value="ENSGAGP00000007633.1"/>
        <property type="gene ID" value="ENSGAGG00000006106.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001894">
        <property type="entry name" value="Cathelicidin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10206">
        <property type="entry name" value="CATHELICIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10206:SF0">
        <property type="entry name" value="CATHELICIDIN B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00666">
        <property type="entry name" value="Cathelicidins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019480865" evidence="6">
        <location>
            <begin position="21"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="167" mass="18520" checksum="6E1D1463AAAD7909" modified="2019-05-08" version="1">MAVVLFLVALLASLGVSVTASPLLEAQLLSPRDVARSAVEAYNQETGTQAVFRLFKLKSFHKTQFDWGTHFSINFTIKETNCRKSVASYRLEDCRYQPNGITRDCSADVSVLDFVQEAPLTSVKCTQQQRAKKPSKKPKPKAQTVPKVTVEYFPSSFSTAALTIPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0U2</accession>
    <name>A0A452H0U2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rab GDP dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Regulates the GDP/GTP exchange reaction of most RAB proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Promotes the dissociation of GDP-bound Rab proteins from the membrane and inhibits their activation. Promotes the dissociation of RAB1A, RAB3A, RAB5A and RAB10 from membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the Rab GDI family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000008060"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009286.1">
        <property type="protein sequence ID" value="ENSGAGP00000008060.1"/>
        <property type="gene ID" value="ENSGAGG00000006411.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005093">
        <property type="term" value="F:Rab GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000001">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.519.10:FF:000005">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.519.10:FF:000014">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000232">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000806">
        <property type="entry name" value="RabGDI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF3">
        <property type="entry name" value="RAB GDP DISSOCIATION INHIBITOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00892">
        <property type="entry name" value="RABGDI"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 8" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="452" mass="51318" checksum="2817BA2BEF787703" modified="2019-05-08" version="1">MHPVGDHVGEREEGSAHGPEPLLWRGXESSSITPLEELYKRFGLPEGPPESMGRGRDWNVDLIPKFLMANGQLVKMLLYTEVTRYLDFKVVEGSFVYRGGQVHKVPSTETEALASNLMGMFEKRRFRKFLVFVANFDENEXKRRFRKFLVFVANFDENEPQTLEGVDPHRTPMRDVYEHFDLGQDVIDFTGHALALHRTDNYLDQPCIETINRIKLYSESLARYGKSPYLYPLYGLGELPQGFARLSAIYGGTYMLNKPVDEIVMENGRVVGVRSEGEVARCKQLICDPSYVPERVRGAGRVVRVVCILSHPIRGTSEASSCQIILPQNQLGRKSDIYVCLVSWAHNVAAPGKFIAIVSTTVETSEPEQEVEPALQLLEPIDQKFVAISDLYEPMDDGTESQIFCSRSYDATTHFETTCDDIKNIYRRMVGAAFDFESMKRHQSHVFGEDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H179</accession>
    <name>A0A452H179_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphoribosylformylglycinamidine synthase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H179"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008351"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009614.1">
        <property type="protein sequence ID" value="ENSGAGP00000008351.1"/>
        <property type="gene ID" value="ENSGAGG00000006639.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.750:FF:000001">
        <property type="entry name" value="Putative phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.750">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040707">
        <property type="entry name" value="FGAR-AT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18076">
        <property type="entry name" value="FGAR-AT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109736">
        <property type="entry name" value="FGAM synthase PurL, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase N-terminal" evidence="7">
        <location>
            <begin position="78"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase linker" evidence="6">
        <location>
            <begin position="181"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="363"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="363"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="49279" checksum="66DBDB89F74F94E2" modified="2019-05-08" version="1">AMAVLHYYQRPGAESVPVSLLRSAQAVLGGVRALHRELCYNVSWTGASPPSPQETQLLHWLFGCPLQSGDVAAESFLRPAPTDLLMEIGPRLNFSTAFSTNVASVCRAVGLGCVERVERAGLRGACRALPPGEGWEAALEGVLSDRMTEQRYEEPIRSFAVAARPAPTWHVDVLGGGRTALEGASRELGLAFDSWDLDFYTELFTRLGRNPTSVECFDLAQSNSEHSRHWFFKGRLLVDGKEVAESLFGSIMRTQESSNPNNVIKFSDNSSAVQGRAVCSLWPRDPSRPSCFEKRTSTRHVVFTAETHNFPTGVAPFSGAATGAGGRIRDVQCTGRGAHVVAGTAGYSFGNLHIPGESWSPACPPVPGLPPRARPAPQGLPPEGARDAPRRGQPVPGLPRSLAPPRGPLAAGGSVCGDPRSVSGWGTGSLPTSRPGVALPPEVRPPPAGGPQGQRWRLGLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1V2</accession>
    <name>A0A452H1V2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc transporter ZIP6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Solute carrier family 39 member 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Zrt- and Irt-like protein 6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Zn(2+)(in) = Zn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29351"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H1V2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008439"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009715.1">
        <property type="protein sequence ID" value="ENSGAGP00000008439.1"/>
        <property type="gene ID" value="ENSGAGG00000006710.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140410">
        <property type="term" value="F:monoatomic cation:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071578">
        <property type="term" value="P:zinc ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050799">
        <property type="entry name" value="ZIP_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191:SF22">
        <property type="entry name" value="ZINC TRANSPORTER ZIP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019009046" description="Zinc transporter ZIP6" evidence="14">
        <location>
            <begin position="23"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="299"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="619"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="646"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="685"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="429"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="716" mass="80064" checksum="EF8547BC5F95A8A4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H1X3</accession>
    <name>A0A452H1X3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Probable ATP-dependent RNA helicase DDX56</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">DEAD box protein 56</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Nucleolar RNA helicase that plays a role in various biological processes including innate immunity, ribosome biogenesis or nucleolus organization. Plays an essential role in maintaining nucleolar integrity in planarian stem cells. Maintains embryonic stem cells proliferation by conventional regulation of ribosome assembly and interaction with OCT4 and POU5F1 complex. Regulates antiviral innate immunity by inhibiting the virus-triggered signaling nuclear translocation of IRF3. Mechanistically, acts by disrupting the interaction between IRF3 and importin IPO5. May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">May form homooligomeric complexes. Interacts with IRF3. Interacts with OCT4 and POU5F1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452H1X3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008558"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009847.1">
        <property type="protein sequence ID" value="ENSGAGP00000008558.1"/>
        <property type="gene ID" value="ENSGAGG00000006789.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17961">
        <property type="entry name" value="DEADc_DDX56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000939">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001046">
        <property type="entry name" value="Probable ATP-dependent RNA helicase ddx56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF21">
        <property type="entry name" value="DEAD-BOX HELICASE 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="5"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="36"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="244"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="510"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="17">
        <location>
            <begin position="5"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="539"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="560" mass="62755" checksum="96DD1049AAE935C3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2I5</accession>
    <name>A0A452H2I5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H2I5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008839"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010165.1">
        <property type="protein sequence ID" value="ENSGAGP00000008839.1"/>
        <property type="gene ID" value="ENSGAGG00000006994.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001119">
        <property type="entry name" value="zinc finger protein 408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000052">
        <property type="entry name" value="zinc finger protein 546 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001289">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000895">
        <property type="entry name" value="Zinc finger protein 597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000771">
        <property type="entry name" value="zinc finger protein 648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000496">
        <property type="entry name" value="Zinc finger with KRAB and SCAN domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="236"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="264"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="342" mass="39232" checksum="7A395E710CC1D511" modified="2019-05-08" version="1">MWGKGCRWACEHSQSLPHPLAFQKPPAGHRPFPGWQQFICRECGKSFSWWSNPNLVRHQQIHTGDWPLLCGECGKRFCQNPNLVRHQQIHTGEQPHPCPECGKRFRQNPNLVRHQRIHTGEQPHPCPECGKRFRQNPNLIRHQQIHTGDWPLLCGECGKRFCQNPNLVRHQRIHTGEQPHPCPECGKRFRQNPNLTRHQRNHTGERPHRCGVCGKGFAQKPNLVRHQQNHTGKRPHQCGECGKAFAQKPNLVWHQQNHMGERPHRCGECGKGFAQKQHLLKHQSIHEGDRDPRACRAPVGWRGAKQGRGREIAMGAHLSNTSELNSASCHTWAPPVKSVCDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H319</accession>
    <name>A0A452H319_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H319"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008812"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010132.1">
        <property type="protein sequence ID" value="ENSGAGP00000008812.1"/>
        <property type="gene ID" value="ENSGAGG00000006979.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049146">
        <property type="entry name" value="FAM186A_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049144">
        <property type="entry name" value="FAM186A_B_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33590">
        <property type="entry name" value="GLUTENIN, HIGH MOLECULAR WEIGHT SUBUNIT PW212-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33590:SF1">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20865">
        <property type="entry name" value="FAM186A-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20870">
        <property type="entry name" value="FAM186A-B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM186A/B N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="FAM186A/B C-terminal" evidence="3">
        <location>
            <begin position="977"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="268"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="672"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="887"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="772"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="271"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="345"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="675"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="896"/>
            <end position="907"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1241" mass="145855" checksum="82B77A2FA0172C64" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H587</accession>
    <name>A0A452H587_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the hook family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H587"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009668"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011105.1">
        <property type="protein sequence ID" value="ENSGAGP00000009668.1"/>
        <property type="gene ID" value="ENSGAGG00000007632.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030705">
        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010256">
        <property type="term" value="P:endomembrane system organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22225">
        <property type="entry name" value="HkD_Hook1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000024">
        <property type="entry name" value="Hook homolog 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008636">
        <property type="entry name" value="Hook_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043936">
        <property type="entry name" value="HOOK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947">
        <property type="entry name" value="HOOK PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947:SF36">
        <property type="entry name" value="PROTEIN HOOK HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05622">
        <property type="entry name" value="HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19047">
        <property type="entry name" value="HOOK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116907">
        <property type="entry name" value="Hook domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="8"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="169"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="322"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="485"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="721" mass="83677" checksum="BD51090912E0F7ED" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H687</accession>
    <name>A0A452H687_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H687"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010031"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011514.1">
        <property type="protein sequence ID" value="ENSGAGP00000010031.1"/>
        <property type="gene ID" value="ENSGAGG00000007875.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043539">
        <property type="entry name" value="Grb2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46037">
        <property type="entry name" value="PROTEIN ENHANCER OF SEVENLESS 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="55" mass="6101" checksum="DA6EB57FB17DCFE0" modified="2019-05-08" version="1">MIRTGISRHEAENLLMSKEVGSFIIRASQNSPGDFSISVRCVACSPMNTWETKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6I6</accession>
    <name>A0A452H6I6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="6">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H6I6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010316"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011844.1">
        <property type="protein sequence ID" value="ENSGAGP00000010316.1"/>
        <property type="gene ID" value="ENSGAGG00000008087.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF138">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="7">
        <location>
            <begin position="39"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="6">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="172" mass="19158" checksum="A96073427028E2E0" modified="2019-05-08" version="1">MQLSALLSGSVATAFKPRCGVPDHPHISHSRRNKRYALTGQKWRQKHITYRYILIFLKGELDTRRAIRQAFDVWQRVTPLTFEEVPYHEVKSDRKEADIMIFFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDETISQLSQSLSGGYSSLLSASRGILGAITPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H757</accession>
    <name>A0A452H757_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000010520"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012080.1">
        <property type="protein sequence ID" value="ENSGAGP00000010520.1"/>
        <property type="gene ID" value="ENSGAGG00000008229.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019882">
        <property type="term" value="P:antigen processing and presentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1540.10:FF:000002">
        <property type="entry name" value="WD repeat and FYVE domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051944">
        <property type="entry name" value="BEACH_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46108">
        <property type="entry name" value="BLUE CHEESE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46108:SF3">
        <property type="entry name" value="WD REPEAT- AND FYVE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="6">
        <location>
            <begin position="2403"/>
            <end position="2528"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="5">
        <location>
            <begin position="2545"/>
            <end position="2839"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2987"/>
            <end position="3028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="802"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="3194" mass="356805" checksum="37FD173AD976A6B0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H789</accession>
    <name>A0A452H789_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H789"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010594"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012156.1">
        <property type="protein sequence ID" value="ENSGAGP00000010594.1"/>
        <property type="gene ID" value="ENSGAGG00000008277.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15229">
        <property type="entry name" value="7tmA_OR8S1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="208"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="52"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="349" mass="39740" checksum="FA9FBBE1786E21BC" modified="2019-05-08" version="1">TSLQMLIFPELISMENQTRVTEFILLGLSSDPHRQIFLFLVFLVIYLITLVGNVVIMVLIRADFHLHTPMYFFLFHLSFVDTCYSSVTVPKMLMNFLAEHKTISVSGCIAQMFFILFTACAEVFVLSAMAYDRYTAICDPLHYVKKMNKRICFQLVSGAWAIGFSHALLNTVFSFNLYFCGPNQISHFSCELPHLLKLSCAETFTNQVVLLTSIVIFGSSSFLLTLVSYIHIISTIMRIHSAEGRHKVFSTCSSHLIVVGLWIRSAEGRRKAFSTCSSHLIVVSLLYLTAFFQYTKPRSVSSVLLDEMFSVQYSILTPMLNPIIYSLKNKEVKTAVRNILGRFRFLNQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H796</accession>
    <name>A0A452H796_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DnaJ homolog subfamily C member 17</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May negatively affect PAX8-induced thyroglobulin/TG transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H796"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010568"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012130.1">
        <property type="protein sequence ID" value="ENSGAGP00000010568.1"/>
        <property type="gene ID" value="ENSGAGG00000008259.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12429">
        <property type="entry name" value="RRM_DNAJC17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000059">
        <property type="entry name" value="dnaJ homolog subfamily C member 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034254">
        <property type="entry name" value="DNAJC17_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052094">
        <property type="entry name" value="Pre-mRNA-splicing_ERAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44313">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44313:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="J" evidence="10">
        <location>
            <begin position="11"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="191"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="249"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="311" mass="35706" checksum="A0BD3999542429CE" modified="2019-05-08" version="1">MAVDKELLQLDLYALLGVGEKSSDKEVKKAYRQKALTCHPDKNPDNPRAEELFHQLSQALAVLTDAAARVAYDKVRKTKKQAAERTQKLDERRKKVKLDLEAREREAQTHVSEEEEIRITRTLEQEILRLREEGSRQLEEQQKLMRDQIRLEREQRVQGKQDGNGVEGKGTPKLKLRWKCKKDDETKGGYSKEVLLQILQKYGEVLNLVISSKKTGSAVVEFATVKAAEMAVKNEVGLINNPLKISWLEGQPQNNPSTKFPDSTSQPRASQDSVVSERDYESLVMMRMRQAAERQQLIEQLKREDEEESHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9S6</accession>
    <name>A0A452H9S6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gamma-tubulin complex component 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the gamma-tubulin ring complex (gTuRC) consisting of TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 and gamma-tubulin TUBG1 or TUBG2. TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 assemble in a 5:5:2:1:1 stoichiometry; each is associated with a gamma-tubulin, thereby arranging 14 gamma-tubulins in a helical manner. Gamma-tubulin at the first position is blocked by TUBGCP3 at the last position, allowing 13 protafilaments to grow into a microtubule. The gTuRC (via TUBGCP3 and TUBGCP6) interacts with ACTB and MZT1; the interactions form a luminal bridge that stabilizes the initial structure during complex assembly. The gTuRC (via TUBGCP2) interacts with MZT2A/MZT2B and CDK5RAP2 (via CM1 motif); the interactions play a role in gTuRC activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000011524"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013196.1">
        <property type="protein sequence ID" value="ENSGAGP00000011524.1"/>
        <property type="gene ID" value="ENSGAGG00000008896.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1900:FF:000004">
        <property type="entry name" value="gamma-tubulin complex component 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045818">
        <property type="entry name" value="GCP6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF70">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19340">
        <property type="entry name" value="GCP6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-tubulin complex component 6 N-terminal" evidence="12">
        <location>
            <begin position="31"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="11">
        <location>
            <begin position="355"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="10">
        <location>
            <begin position="1470"/>
            <end position="1737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="844"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="890"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1205"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="896"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1216"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1239"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1261"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1281"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1298"/>
            <end position="1312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00071901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1753" mass="199327" checksum="E196DF0CCAC80FC6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9Y7</accession>
    <name>A0A452H9Y7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Insulin-like growth factor-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H9Y7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011447"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013106.1">
        <property type="protein sequence ID" value="ENSGAGP00000011447.1"/>
        <property type="gene ID" value="ENSGAGG00000008831.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001968">
        <property type="term" value="F:fibronectin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031994">
        <property type="term" value="F:insulin-like growth factor I binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031995">
        <property type="term" value="F:insulin-like growth factor II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043567">
        <property type="term" value="P:regulation of insulin-like growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.40.20:FF:000001">
        <property type="entry name" value="Insulin-like growth factor binding protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000005">
        <property type="entry name" value="Putative insulin-like growth factor-binding protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012211">
        <property type="entry name" value="IGFBP-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022321">
        <property type="entry name" value="IGFBP_1-6_chordata"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017891">
        <property type="entry name" value="Insulin_GF-bd_Cys-rich_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11551">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11551:SF3">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01976">
        <property type="entry name" value="IGFBPFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01979">
        <property type="entry name" value="IGFBPFAMILY3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00222">
        <property type="entry name" value="IGFBP_N_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019438057" description="Insulin-like growth factor-binding protein 3" evidence="12">
        <location>
            <begin position="24"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="14">
        <location>
            <begin position="26"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="13">
        <location>
            <begin position="200"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="118"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="281" mass="31141" checksum="1D220601A59CDC11" modified="2019-05-08" version="1">MVARLCALWAVAALALLSRRALAVGPVVRCEPCDSRALEQCKPLAPECSELVREPGCGCCLTCALQEGRPCGVYSERCGAGLSCQPRRGELRPLQALLEGRGLCTNVTTGSKLRTFLLQGPHVPGNSSDSEEDQNASSIENQAIPNSHRVSDSKSHPHHTKIDIIRKGQAKNTQRYKVEYDSQSTDTLNFSSESKQETEYGPCRREMEDTLNHLKIQNVLSPRGFHIPNCDKKGFYKKKQCRPSKGRKRGYCWCVDKYGQPLPGYDGKGKGDVHCYNLESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HA39</accession>
    <name>A0A452HA39_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Mediator of RNA polymerase II transcription subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Mediator complex subunit 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MED9</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9 11">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Mediator complex subunit 9 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HA39"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011584"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013263.1">
        <property type="protein sequence ID" value="ENSGAGP00000011584.1"/>
        <property type="gene ID" value="ENSGAGG00000008943.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21431">
        <property type="entry name" value="Med9-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011425">
        <property type="entry name" value="Med9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039242">
        <property type="entry name" value="MED9_metazoa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20844:SF0">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20844">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07544">
        <property type="entry name" value="Med9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 11" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="140" mass="15889" checksum="B09C760AE7BBDA6F" modified="2019-05-08" version="1">MATGGAGVGVRPVVEEQQQPPPQLQAPQPPEQQGEQKPQLQPPPQQPPSQQPGQEDFSFLPLVHDIIKCMDKDSQDVHQVLNELKTKFQEMRKMISAMPGIGVSPEQQQQQLRSLREQVRTKNELLQKYKSLCMFEIPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HA86</accession>
    <name>A0A452HA86_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA-splicing endonuclease subunit SEN34</fullName>
            <ecNumber evidence="3">4.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13 14">tRNA-intron endonuclease SEN34</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA-splicing endonuclease subunit Sen34</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.</text>
            <dbReference type="EC" id="4.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">tRNA splicing endonuclease is a heterotetramer composed of TSEN2, TSEN15, TSEN34/LENG5 and TSEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3'-end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA-intron endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452HA86"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011633"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013328.1">
        <property type="protein sequence ID" value="ENSGAGP00000011633.1"/>
        <property type="gene ID" value="ENSGAGG00000008982.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.10:FF:000002">
        <property type="entry name" value="tRNA-splicing endonuclease subunit Sen34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059049">
        <property type="entry name" value="TSEN34_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13070:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13070">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26577">
        <property type="entry name" value="TSEN34_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TSEN34 N-terminal" evidence="17">
        <location>
            <begin position="108"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="16">
        <location>
            <begin position="298"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00076863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF26577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="390" mass="42417" checksum="383F568D21677DF0" modified="2019-05-08" version="1">MMGLQCKPPPPSLAPENAGIVPPLTLGNVGIAPSLGWGPGGGQSQGMLGLWRPLPQSMLGLWCPAPEVAASQPWVRGPRITSPCTLPQRGHVPHSRRSPPCSCRSAMIQIHVLEGAALVWSAEDAREIRERFRLVGTLVGALARKPRQNARLGLPLQLLPEEARLLAELGAAVLVRGTETQPPAEGEELTQASLVEAYQRELEESYQEQQRLAGAERGALLARLAERIAQGRARRREQRGEMPDTVEDSGLLDSPFVLPRGSMMVQLPTARWRPGRVEQVDWTSPSADWPHAGRPAHEARYRVFRELWGRGYYLSGGSKFGGDFLVYPGDPLRFHAHYIALCCPPEAPLPLHALVAATRLGTSVKKTLLLCSPGAGSALTFTSLQWSGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBZ6</accession>
    <name>A0A452HBZ6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HBZ6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012289"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014074.1">
        <property type="protein sequence ID" value="ENSGAGP00000012289.1"/>
        <property type="gene ID" value="ENSGAGG00000009453.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000585">
        <property type="entry name" value="WD repeat domain 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042505">
        <property type="entry name" value="DYNC2I1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16022:SF0">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 2 INTERMEDIATE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16022">
        <property type="entry name" value="WD REPEAT DOMAIN 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="651"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="107"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="173"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="316"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="350"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="953" mass="109963" checksum="41F53CB8CCC1A8E6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HC46</accession>
    <name>A0A452HC46_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ETS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HC46"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012355"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014144.1">
        <property type="protein sequence ID" value="ENSGAGP00000012355.1"/>
        <property type="gene ID" value="ENSGAGG00000009495.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF160">
        <property type="entry name" value="ETS TRANSLOCATION VARIANT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019090276" description="ETS domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="ETS" evidence="5">
        <location>
            <begin position="6"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="170" mass="18539" checksum="6D866A24FE183DF5" modified="2019-05-08" version="1">MRRGSLQLWQFLVTLLDDPANAHFIAWTGRGMEFKLIEPEEVRNRTRDLPVAAGLVAIQKQQTSSPFQAKGPSSQTQLLAIHCCCFPGPLPTWASSHLDPLASHLSLGLMFPGRFSPQLKQVQPGSNPGCPSSSYGQRGAPVPLVPAELWLLLGGLSKQAWRTPPLLLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCW1</accession>
    <name>A0A452HCW1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000012634"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014487.1">
        <property type="protein sequence ID" value="ENSGAGP00000012634.1"/>
        <property type="gene ID" value="ENSGAGG00000009703.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06756">
        <property type="entry name" value="PDZ_ARHGAP21_23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01253">
        <property type="entry name" value="PH_ARHGAP21-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04395">
        <property type="entry name" value="RhoGAP_ARHGAP21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000014">
        <property type="entry name" value="Rho GTPase activating protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000066">
        <property type="entry name" value="Rho GTPase activating protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23175">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23175:SF5">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="132"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="922"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="5">
        <location>
            <begin position="1131"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="290"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="471"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="572"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="653"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1056"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1350"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1401"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1504"/>
            <end position="1760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="335"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="511"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="574"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="601"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="671"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="709"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1062"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1363"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1634"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1677"/>
            <end position="1686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1704"/>
            <end position="1723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1760" mass="193375" checksum="58006B1C8E4DD82D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEQ2</accession>
    <name>A0A452HEQ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acyl-CoA-binding domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Acyl-CoA binding protein which acts as the peroxisome receptor for pexophagy but is dispensable for aggrephagy and nonselective autophagy. Binds medium- and long-chain acyl-CoA esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG37 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HEQ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013338"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015266.1">
        <property type="protein sequence ID" value="ENSGAGP00000013338.1"/>
        <property type="gene ID" value="ENSGAGG00000010206.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00435">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000010">
        <property type="entry name" value="Acyl-CoA-binding domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016347">
        <property type="entry name" value="ACBD5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022408">
        <property type="entry name" value="Acyl-CoA-binding_prot_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF6">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002412">
        <property type="entry name" value="MA_DBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00880">
        <property type="entry name" value="ACB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="ACB" evidence="15">
        <location>
            <begin position="8"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="144"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="348"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="179"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="348"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="393"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="404"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>an acyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="39"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>an acyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>an acyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>an acyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR002412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR002412-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="505" mass="56183" checksum="85B3C51025EA6D9A" modified="2019-05-08" version="1">MAETGSVHETRFEAAVKVIQSLPKNGSFQPSNEMMLKFYSFYKQATQGPCNSPRPGFWDPIGRYKWDAWSALGDMPKEEAMIAYVEEMKKILESMPMTEKVEELLQVIGPFYEIVEDKKNRGSGLTSVRLEKVSKYLEDLGNVMTSTPNSKAVNGKAESNDSGAESEEEQGHEEEEEKEIQQSEKDYKNVKQDSTASKDLESIVTNGCYQDNFVTEMQNGIQTKSALNGLNVEEEITKVEQSLALMGKTNNSGHQGSNEDSVEEVSGIQHLTSDSDSEVFCDSMEQFGQEEPLEMITSAKGPLQHSSHFLEVDHSHLLENAGFPEHAYLPSENFRVEDITEAAVEGKGEVKCGGEDGKSSNGGPHKEKKGGEKVDFHGVRRGRGHRMQPLGDGAQGGQMGSGGDGERWGSDRAPRGNLNEQIAVVLMRLQEDMQNVLQRLHTLEALTASQARSVTLQSNLQPASCVKRPSWWPFDISPGTLAFAVVWPFVAQWLVHVYFQRRRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFC5</accession>
    <name>A0A452HFC5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Thyrotropin-releasing hormone-degrading ectoenzyme</fullName>
            <ecNumber evidence="18">3.4.19.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Pyroglutamyl-peptidase II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">TRH-specific aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Thyroliberinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Specific inactivation of TRH after its release.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Release of the N-terminal pyroglutamyl group from pGlu-|-His-Xaa tripeptides and pGlu-|-His-Xaa-Gly tetrapeptides.</text>
            <dbReference type="EC" id="3.4.19.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="24">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="24">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.6" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A452HFC5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013563"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015524.1">
        <property type="protein sequence ID" value="ENSGAGP00000013563.1"/>
        <property type="gene ID" value="ENSGAGG00000010357.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016920">
        <property type="term" value="F:pyroglutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000006">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000005">
        <property type="entry name" value="Aminopeptidase N-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000039">
        <property type="entry name" value="thyrotropin-releasing hormone-degrading ectoenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000007">
        <property type="entry name" value="thyrotropin-releasing hormone-degrading ectoenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF294">
        <property type="entry name" value="THYROTROPIN-RELEASING HORMONE-DEGRADING ECTOENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 27" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9 24" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="30">
        <location>
            <begin position="150"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="28">
        <location>
            <begin position="371"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="29">
        <location>
            <begin position="681"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="101"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="23">
        <location>
            <position position="444"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="25">
        <location>
            <position position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00076496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00079759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00083577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1027" mass="118527" checksum="1294300FA965FA5C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGU9</accession>
    <name>A0A452HGU9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">proteasome endopeptidase complex</fullName>
            <ecNumber evidence="3">3.4.25.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452HGU9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014085"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016128.1">
        <property type="protein sequence ID" value="ENSGAGP00000014085.1"/>
        <property type="gene ID" value="ENSGAGG00000010588.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03763">
        <property type="entry name" value="proteasome_beta_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF4">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0888">Threonine protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="172" mass="18225" checksum="18C9E2754295670E" modified="2019-05-08" version="1">MAAVSVLQAPSSGFGFENCARNALLEAELTKKGLRLPAARKTGTTIAGVVFKDGIVLGADTRATEGMIVADKNCSKIHFISPNIYCCGAGTAADTDMTTQLISSNLELHSLSTGRLPRVVTANRMLKQMLFRYQGYIGAALVLGGVDVSGPHLYSIYPHGSTDKLPYVTMGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHX9</accession>
    <name>A0A452HHX9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycogen synthase kinase-3 beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HHX9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014510"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016598.1">
        <property type="protein sequence ID" value="ENSGAGP00000014510.1"/>
        <property type="gene ID" value="ENSGAGG00000011028.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030877">
        <property type="term" value="C:beta-catenin destruction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF8">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15615" checksum="BC7491490A1E8F20" modified="2019-05-08" version="1">MNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGREKPALFNFTTQELSSNPSLASILIPAHARNQAAASTPTNATAASGENMRLWTFSPQILLACKLYSLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HK58</accession>
    <name>A0A452HK58_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HK58"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015302"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017479.1">
        <property type="protein sequence ID" value="ENSGAGP00000015302.1"/>
        <property type="gene ID" value="ENSGAGG00000011541.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="119" mass="13134" checksum="47E54E98F0B47356" modified="2019-05-08" version="1">MALENAFDNFLVIVVDSGKLLDPQEFLLTGAQRQLELKGLITGIGYEVMLYGFAKGRQTKPLSTVAVTGILSWHDVHVCLFNFCPSHSFAPTTLTKISFLCNLLHLMEHALLGGLVHHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKG7</accession>
    <name>A0A452HKG7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HKG7"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017612.1">
        <property type="protein sequence ID" value="ENSGAGP00000015417.1"/>
        <property type="gene ID" value="ENSGAGG00000011639.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="75" mass="8695" checksum="68081DA096461AE0" modified="2019-05-08" version="1">QGQGDFSLLKSLSNEKARLGKERRKVEELQRQHAESQTHLETQPESMRERMQSQLQQVGAGDSPNWKMCREGQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMJ8</accession>
    <name>A0A452HMJ8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HMJ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016167"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018459.1">
        <property type="protein sequence ID" value="ENSGAGP00000016166.1"/>
        <property type="gene ID" value="ENSGAGG00000012137.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000018460.1">
        <property type="protein sequence ID" value="ENSGAGP00000016167.1"/>
        <property type="gene ID" value="ENSGAGG00000012137.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd11921">
        <property type="entry name" value="SH3_Vinexin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11924">
        <property type="entry name" value="SH3_Vinexin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11918">
        <property type="entry name" value="SH3_Vinexin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000001">
        <property type="entry name" value="Sorbin and SH3 domain-containing protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003127">
        <property type="entry name" value="SoHo_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035609">
        <property type="entry name" value="Vinexin_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035608">
        <property type="entry name" value="Vinexin_SH3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035607">
        <property type="entry name" value="Vinexin_SH3_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF54">
        <property type="entry name" value="VINEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02208">
        <property type="entry name" value="Sorb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00459">
        <property type="entry name" value="Sorb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50831">
        <property type="entry name" value="SOHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0965">Cell junction</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SoHo" evidence="8">
        <location>
            <begin position="242"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="519"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="593"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="824"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="448"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="661"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="740"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="688"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="706"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="740"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="753"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="768"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="785"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="883" mass="96041" checksum="599096907A55091D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HNP0</accession>
    <name>A0A452HNP0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HNP0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016615"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018968.1">
        <property type="protein sequence ID" value="ENSGAGP00000016615.1"/>
        <property type="gene ID" value="ENSGAGG00000012429.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050413">
        <property type="entry name" value="TCR_beta_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268:SF31">
        <property type="entry name" value="T CELL RECEPTOR BETA VARIABLE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268">
        <property type="entry name" value="T-CELL RECEPTOR BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="115" mass="12998" checksum="9B1633BC0AF3A768" modified="2019-05-08" version="1">QTLHQTPRFISLPPGSSIQLHCYLQGYSSTPWMYWYRQPPQGGALHMLFLSRGKDMIDDSSEKHFKAARPTQQNFTLSTDSLFPNHTGLYYCAWSRTLSQAGAVPGQKLPLPLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQS1</accession>
    <name>A0A452HQS1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQS1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017394"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019843.1">
        <property type="protein sequence ID" value="ENSGAGP00000017394.1"/>
        <property type="gene ID" value="ENSGAGG00000012942.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020443">
        <property type="entry name" value="IL-10/19/20/24/26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020444">
        <property type="entry name" value="IL-24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48482:SF3">
        <property type="entry name" value="INTERLEUKIN-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48482">
        <property type="entry name" value="INTERLEUKIN-19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01937">
        <property type="entry name" value="INTRLEUKIN24"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019559255" evidence="6">
        <location>
            <begin position="25"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="162" mass="18601" checksum="AB87228A0648C6A6" modified="2019-05-08" version="1">MKISCLSLYLLSTMCWLYLTPTTGNKIFQFGPCMISMNINEIRASFTAIKATIVSIAPYTISLKPADRCCVIHHLLRFYVDKVFKHCETEDSHVNRKISSIANSFLSIKKKFRQCVPFRSFQIAVIIYQIFLSIYFQLNTASAAMKSLGELDILLDWMEKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HSU0</accession>
    <name>A0A452HSU0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Epithelial splicing regulatory protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">RNA-binding motif protein 35B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">RNA-binding protein 35B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESRP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HSU0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018148"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020686.1">
        <property type="protein sequence ID" value="ENSGAGP00000018148.1"/>
        <property type="gene ID" value="ENSGAGG00000013442.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12740">
        <property type="entry name" value="RRM2_ESRP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12742">
        <property type="entry name" value="RRM3_ESRP1_ESRP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000041">
        <property type="entry name" value="Epithelial splicing regulatory protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000070">
        <property type="entry name" value="Epithelial splicing regulatory protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000056">
        <property type="entry name" value="epithelial splicing regulatory protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000037">
        <property type="entry name" value="epithelial splicing regulatory protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="228"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="708" mass="77812" checksum="2563A5C9C520531E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HU07</accession>
    <name>A0A452HU07_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Deubiquitinase DESI2</fullName>
            <ecNumber evidence="4">3.1.2.22</ecNumber>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Desumoylating isopeptidase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">PPPDE peptidase domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Palmitoyl protein thioesterase DESI2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Protein FAM152A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">S-depalmitoylase DESI2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:19234"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DeSI family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="4"/>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452HU07"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018609"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021215.1">
        <property type="protein sequence ID" value="ENSGAGP00000018609.1"/>
        <property type="gene ID" value="ENSGAGG00000013769.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF6">
        <property type="entry name" value="DEUBIQUITINASE DESI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="PPPDE" evidence="18">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="177" mass="19494" checksum="B5B739B275C58E5A" modified="2019-05-08" version="1">MNEYTSSLGIGVFHSGIEVYGREFAYGGHPYPFSGIFEISPGNAAELGETFKFKEAVVLGSTDFMEDDIEKIVEELGKEFKGNAYHLMHKNCNHFSSALSEILCGKEIPRWVNRLAYFSSCIPFLQSCLPKEWLTPAALQSSVSQELQDELEEAEDAAAASLSNSASGSRTGRHTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HUZ0</accession>
    <name>A0A452HUZ0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ITPRIP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mab-21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HUZ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018895"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021541.1">
        <property type="protein sequence ID" value="ENSGAGP00000018895.1"/>
        <property type="gene ID" value="ENSGAGG00000013987.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026250">
        <property type="entry name" value="ITPRIP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046903">
        <property type="entry name" value="Mab-21-like_nuc_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046906">
        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF9">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR-INTERACTING PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03281">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20266">
        <property type="entry name" value="Mab-21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02107">
        <property type="entry name" value="INOS145TPRIP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Mab-21-like nucleotidyltransferase" evidence="8">
        <location>
            <begin position="159"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Mab-21-like HhH/H2TH-like" evidence="9">
        <location>
            <begin position="356"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="528" mass="58717" checksum="3C9CE13ED8F176F2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVQ3</accession>
    <name>A0A452HVQ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4592 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HVQ3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019228"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021906.1">
        <property type="protein sequence ID" value="ENSGAGP00000019228.1"/>
        <property type="gene ID" value="ENSGAGG00000014212.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026713">
        <property type="entry name" value="CRACD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028030">
        <property type="entry name" value="DUF4592"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47743:SF1">
        <property type="entry name" value="CRACD-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47743">
        <property type="entry name" value="KIAA1210 / KIAA1211 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15262">
        <property type="entry name" value="DUF4592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4592" evidence="2">
        <location>
            <begin position="130"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="742"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="523"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="743"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="777"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="787"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="846"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="904"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="924"/>
            <end position="936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="978" mass="107912" checksum="03331CF609431813" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HYL6</accession>
    <name>A0A452HYL6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 40</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HYL6"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023098.1">
        <property type="protein sequence ID" value="ENSGAGP00000020275.1"/>
        <property type="gene ID" value="ENSGAGG00000014952.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026181">
        <property type="entry name" value="TMEM40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16108">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16108:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15817">
        <property type="entry name" value="TMEM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19340" checksum="5AF36BE44476954E" modified="2019-05-08" version="1">HTEIASNSYNTSLSFLSLLATSAGKKKVSVATVKPISHNQSQEDQDHENQDGSGAPGEIVPDNESEIARRENATAAAGEKITYLQRCQRQWIRIRKNDVFFHFIILCFGIGAALIGYYHYKDWTISLGFGLITFASLETTGIYFGLVHRIHSVLEGFIPLIQRFKIPGIRKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HZ54</accession>
    <name>A0A452HZ54_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ferlin family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000020505"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023358.1">
        <property type="protein sequence ID" value="ENSGAGP00000020505.1"/>
        <property type="gene ID" value="ENSGAGG00000004680.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04017">
        <property type="entry name" value="C2D_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04037">
        <property type="entry name" value="C2E_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08374">
        <property type="entry name" value="C2F_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000009">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000021">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000026">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037723">
        <property type="entry name" value="C2D_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037724">
        <property type="entry name" value="C2E_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037725">
        <property type="entry name" value="C2F_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037721">
        <property type="entry name" value="Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012560">
        <property type="entry name" value="Ferlin_A-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012561">
        <property type="entry name" value="Ferlin_B-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032362">
        <property type="entry name" value="Ferlin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055072">
        <property type="entry name" value="Ferlin_DSRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546">
        <property type="entry name" value="FER-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546:SF55">
        <property type="entry name" value="MYOFERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22901">
        <property type="entry name" value="dsrm_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08165">
        <property type="entry name" value="FerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08150">
        <property type="entry name" value="FerB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16165">
        <property type="entry name" value="Ferlin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00693">
        <property type="entry name" value="DysFN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01200">
        <property type="entry name" value="FerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01201">
        <property type="entry name" value="FerB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1579"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="679"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="842"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="1088"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="1322"/>
            <end position="1471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1659" mass="190330" checksum="C75E63D3977F1CD4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0Z0</accession>
    <name>A0A452I0Z0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Probable G-protein coupled receptor 33</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Orphan receptor; could be a chemoattractant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the chemokine-like receptor (CMKLR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I0Z0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021131"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024068.1">
        <property type="protein sequence ID" value="ENSGAGP00000021131.1"/>
        <property type="gene ID" value="ENSGAGG00000015518.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004875">
        <property type="term" value="F:complement receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000034">
        <property type="entry name" value="G-protein coupled receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000826">
        <property type="entry name" value="Formyl_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225">
        <property type="entry name" value="CHEMOTACTIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225:SF5">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00526">
        <property type="entry name" value="FMETLEUPHER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="44"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="364" mass="40537" checksum="46F160A378E20255" modified="2019-05-08" version="1">MDRGNMTLQPTTGENSSQTLAAVSATHLATAVLLFTTFLVGVVGNGLYLWVLGLKMRRTVTTLMFLHLVSSYLLLTLLIPFFAVYVLLGFHWVFGMAMCKLLNACISVGMFTSVFLLTLISLDRYTLTHRPIWCRHHRTVPWVRKLVVGVWLVSLALSAPYLAFRETRVVDGGRITCTNNYTLSGIWNGAETQDQGRRVHLAVLMVRFLLGFLLPFCTIAGCYGCVGLEMKEKGLARSRKPFKVMVAAVVSFFLSWLPYHLYHGLKFFKDGWELDSILVIYTLTSCFNACFTPVLYLFVGRRFQQVLKTSLLALLRETFAEDLSGNGAISHESSGVVAGKLELPEQVMGPLDSGSGSLEPRVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3R3</accession>
    <name>A0A452I3R3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I3R3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022160"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025259.1">
        <property type="protein sequence ID" value="ENSGAGP00000022160.1"/>
        <property type="gene ID" value="ENSGAGG00000016277.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031486">
        <property type="entry name" value="TMEM215"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31922">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31922:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15746">
        <property type="entry name" value="TMEM215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="235" mass="25826" checksum="3D9E7B4DCF187CF3" modified="2019-05-08" version="1">MRPDDINPRTGLVVALVSVFLVFGFMFTVSGMKGETLGDIPLLAIGPAICLPGIAAIALTRKTDGCTKWPDNKRPCCKKAKYKEVVELLRTPSDLESGKSSCDELAKKAYLKDRKVLKGEDSVSICTTTTTTTTTGECKSLIKRVDQDEMLRYLETCYPEMPGNVLVGEGPTYSALEKKNFPPWDSAACPDTEDNIFVAPKDSIIVCSYKENSPYDRYCCYINPTGVSPDHETIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I408</accession>
    <name>A0A452I408_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I408"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025376.1">
        <property type="protein sequence ID" value="ENSGAGP00000022265.1"/>
        <property type="gene ID" value="ENSGAGG00000016355.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001230">
        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="131" mass="14345" checksum="49B35389D6EFD43C" modified="2019-05-08" version="1">MTQTPESLAVLPGDRVTINCKASSSITSGSTHILAWYQQKTGQAPKLLIYYASTRATGIPDRFTGSGSGTDFTLTISNVQAEDAGDYYCQQGYSWGLGTGLITLSHKIQNILQRQRWRNRPLLGVTGFRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I433</accession>
    <name>A0A452I433_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I433"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022298"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025413.1">
        <property type="protein sequence ID" value="ENSGAGP00000022298.1"/>
        <property type="gene ID" value="ENSGAGG00000016308.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02997">
        <property type="entry name" value="PDI_a_PDIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000029">
        <property type="entry name" value="protein disulfide-isomerase A5 isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046374">
        <property type="entry name" value="PDI_a_PDIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF2">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="115"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="237"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="362"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="496" mass="57266" checksum="CA25AB36BABE6BCB" modified="2019-05-08" version="1">WCFSVRSPKELLLIEFSYLQLLYLLIGLMLLLLLFSAAAAENPLRFLSDVAQEVKGRGTIAWIDCGDTESRKVCKKMKADPNSKEKGVELLHYKDGVFHTEYNRAVTLKSVVAFLKDPEGAPLWEEDPEAKDVVHVDSEKEFRRLLKKEDRPLLMMFYAPWCGVCKRMMPSYQQAATELKGTYVLAGMNVHSTEFEKIKEDYNVRGYPTICYFEKGKFLFHYENYGATAKDIAEWLQNPQPPQPQAPETPWADEENAVYHLTDEDFDKFIKEHSSVLVMFHAPWCGHCKKMKPEYEMALSLFQSPGVLAAVDATVNKAVAERFHISGFPTLKYFQDGEEKYTLPQLRTKKKIVDWLQNPQAPPPPELAWEEKLTNVIHLAGEDFRESLKKKKHALVMFYAPWCPHCKNSIPHFTTAAELFKEDRKIAYAAVDCAKEQNHDLCKQEGVDGYPTFNYYNYGKFVEKYNGERGESGFATFMRTLRERDHERVGKKKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4L3</accession>
    <name>A0A452I4L3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I4L3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022483"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025613.1">
        <property type="protein sequence ID" value="ENSGAGP00000022483.1"/>
        <property type="gene ID" value="ENSGAGG00000016490.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF18">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="7"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="133" mass="14988" checksum="C6921DCA8258EFAA" modified="2019-05-08" version="1">YLLLKIPLDSPMEVTVSEGHDVLLQCNFTSISSSPNLFWYRQYPNQPPQHILTAYKSPAEKNTFTSEKFSTVLFDANKTVPLKIEAVSVQDKAVYYCGLRPTLGQSCISGVTRSDRGAYTHRRLGDCFSTRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4Z8</accession>
    <name>A0A452I4Z8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I4Z8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022650"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025804.1">
        <property type="protein sequence ID" value="ENSGAGP00000022650.1"/>
        <property type="gene ID" value="ENSGAGG00000016609.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="244" mass="27226" checksum="1E26562BCE3FE7FF" modified="2019-05-08" version="1">SPTTRWSTSAGAMSGPLDARSPPGPGNGRGALANLPNRLPHSAQLYGRSPVCARWCRLRLELSAKLLPQSVHWKGLSPLCVRWCLASALVLLKFLLQTSQRCGGCPLCVCWCVLKFELSAKLLPQSAQLYGRSPVCVRWCLLRLELCAKPLPQSAQWYGRSPVCVRRCLLRIELCAKHFPQSAQWYGRSPVSCVYWCWVSAEGLLKLLPQSMQQWGRWPMGSLWCVARSSLWLSLAWGWTASCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5B8</accession>
    <name>A0A452I5B8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I5B8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022780"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025953.1">
        <property type="protein sequence ID" value="ENSGAGP00000022780.1"/>
        <property type="gene ID" value="ENSGAGG00000016703.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22100">
        <property type="entry name" value="F-box_FBXO28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039719">
        <property type="entry name" value="FBXO28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13252">
        <property type="entry name" value="F-BOX ONLY PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13252:SF9">
        <property type="entry name" value="F-BOX ONLY PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="44"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="312"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="351" mass="39418" checksum="DBFEF977BF9D91CB" modified="2019-05-08" version="1">MAAVAEERLLSEGEGGAMGSARLSPPPVPQLQGGPAPLEQLPQSNTLVGLPIVAIESILGFLSYDETSQLRLVCKRMDLVCQRMLNQGFLKVERYHNLCQKQVKAQLPRRESERRNHSLARHADILAAVETRLSLLNMTFMKYVDSNLCCFIPGKVIDEIYRVLRYVNSTRAPQRAHEVLQELRDISSMAMEYFDEKIVPILKRKLPGSDVSGRLIGAAPVPGPSAALTTMQLFSKQNPSRQEVTKLQQQVKTNGAGVTVLRREISELRTKVQEQQKQLQDQDQKLLEQTQIIGEQNARLAELERKLREVMESAVGSSSGSGSNEESLRKRRKAVESIDSPRKSKRLRNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5M4</accession>
    <name>A0A452I5M4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Torsin-1A-interacting protein 1/2 AAA+ activator domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TOR1AIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I5M4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022870"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026054.1">
        <property type="protein sequence ID" value="ENSGAGP00000022868.1"/>
        <property type="gene ID" value="ENSGAGG00000016766.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000026056.1">
        <property type="protein sequence ID" value="ENSGAGP00000022870.1"/>
        <property type="gene ID" value="ENSGAGG00000016766.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038599">
        <property type="entry name" value="LAP1C-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008662">
        <property type="entry name" value="TOIP1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046753">
        <property type="entry name" value="TOIP1/2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18843">
        <property type="entry name" value="TORSIN-1A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18843:SF2">
        <property type="entry name" value="TORSIN-1A-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05609">
        <property type="entry name" value="LAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Torsin-1A-interacting protein 1/2 AAA+ activator" evidence="11">
        <location>
            <begin position="257"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="141"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="63"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="177"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="493" mass="54840" checksum="2616F3069DB081FB" modified="2019-05-08" version="1">MAENGDQDPHWTYPAQELVRREEERTPQAQEGENVLRTHQEKMIEDSGSVQLKTSPEIETLGPQETNSVSGSPSAVNEEDSSEGRPVSSDGISLRTNTEESPGLLNLNTMSLPDYSLDKDMVSSLGEWKTHSGPVALEEKIIREPPTEEENCIPTPRGEPQDTIIQETTLPSADFLEPVKESISNKEEERPQDTARKRHLTEAGKSEAEAHPQETETRQEATPEKEKSFLGTGLAFPAASLGFLVVVVCFSIYCNGYYTSQPHYVPENPALEAFLSRFNQLKDSFPGQSSYLWARGRKLLQRHLNTSSHSEPAILIFTAAREGEETLKCLSNCIADAYSTSLRASTIQVDGAGKATLNSDKAKLEVDYELSTGFEEGKRAAVVHRFESLPAGSTLIFYKYCDHESAAFKDVALLLTVLLEEEKLEANIHLQQVEERVRDFLWAKFTNANTPSSYNHMDTDKLSGLWSRISHLVLPIHPVKAIENNGCSFQTKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I5N8</accession>
    <name>A0A452I5N8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000022908"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026101.1">
        <property type="protein sequence ID" value="ENSGAGP00000022908.1"/>
        <property type="gene ID" value="ENSGAGG00000016797.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595:SF1">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="7"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="234" mass="25220" checksum="B85652E3E7E7BEAB" modified="2019-05-08" version="1">FASASSPAWNTSELQNLISVWGEEAVQSQLRSSRRNYDTYGQISKSLQRRGHEWDALQCRVKVKELQSAYCKAREGNRHSGAAPTTCRFYKELDAILGCDPTANPRTTMEKEAESEAPGVEGDTLESQEACSQGLFSSQEEASQSDDRSSRIISFPGCCRHLGRGSWGRGARRGPPAASGRGAAHRGTPRPSSTLPHLLPEHAMAASLPPPPRLAAPKLIGAASLGXXXXXXXV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6F2</accession>
    <name>A0A452I6F2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RING finger protein 17</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Seems to be involved in regulation of transcriptional activity of MYC. In vitro, inhibits DNA-binding activity of Mad-MAX heterodimers. Can recruit Mad transcriptional repressors (MXD1, MXD3, MXD4 and MXI1) to the cytoplasm. May be involved in spermiogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with MXD1, MXD3, MXD4, MXI1 and PIWIL1. Self-associates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000023188"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026422.1">
        <property type="protein sequence ID" value="ENSGAGP00000023188.1"/>
        <property type="gene ID" value="ENSGAGG00000016999.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20414">
        <property type="entry name" value="Tudor_TDRD4_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20415">
        <property type="entry name" value="Tudor_TDRD4_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20416">
        <property type="entry name" value="Tudor_TDRD4_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20417">
        <property type="entry name" value="Tudor_TDRD4_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000114">
        <property type="entry name" value="RING finger protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047845">
        <property type="entry name" value="RNF17-like_TUDOR_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047847">
        <property type="entry name" value="RNF17-like_TUDOR_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047848">
        <property type="entry name" value="RNF17-like_TUDOR_rpt3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047849">
        <property type="entry name" value="RNF17-like_TUDOR_rpt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16442">
        <property type="entry name" value="RING FINGER PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16442:SF1">
        <property type="entry name" value="RING FINGER PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Tudor" evidence="15">
        <location>
            <begin position="812"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="15">
        <location>
            <begin position="1048"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="15">
        <location>
            <begin position="1316"/>
            <end position="1373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1588" mass="180218" checksum="EEF63BE5DF30FE33" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6I7</accession>
    <name>A0A452I6I7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I6I7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023185"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026419.1">
        <property type="protein sequence ID" value="ENSGAGP00000023185.1"/>
        <property type="gene ID" value="ENSGAGG00000016996.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03593">
        <property type="entry name" value="CLECT_NK_receptors_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050828">
        <property type="entry name" value="C-type_lectin/matrix_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033992">
        <property type="entry name" value="NKR-like_CTLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710:SF35">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 2 MEMBER D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="108"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="229" mass="25813" checksum="2DF853B6DE84EF8D" modified="2019-05-08" version="1">MDPESQRVVASEIELFGVQHAPQGLQEKGEMEEHFHCSGGVERGPGSNHTLRKCASRRVLVPAAVTVFMLIIIIAVLAVLLRVEKFKAPLAAPDPPAVPCCPDGWIGYRGKCYYFSETDGNWTYSQSQCSALNASLAGIDSEQEKDFLLRYKGFLDRWIGLQRKLGQPWRWPNGTEFDNRFQIRGGGDCVFLIDEDWFGSSRCRTWRHWICSKPDTHTMGKGYAVESEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7R4</accession>
    <name>A0A452I7R4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I7R4"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026860.1">
        <property type="protein sequence ID" value="ENSGAGP00000023572.1"/>
        <property type="gene ID" value="ENSGAGG00000017280.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="108" mass="11904" checksum="ED1FDCCABB99BFE8" modified="2019-05-08" version="1">MGAQSIPDFCCPGTSGTSCGRCTVWRSVLILQPLTVIDLFRGKWLTSVWRPLSLKASSYLRNIFNARHLVVHHMPQKPLPPLYPQLTLQHQTVHLAPAVVGALSGGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8A0</accession>
    <name>A0A452I8A0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 92 protein</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 92 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A452I8A0"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027198.1">
        <property type="protein sequence ID" value="ENSGAGP00000023878.1"/>
        <property type="gene ID" value="ENSGAGG00000017476.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008166">
        <property type="entry name" value="Glyco_transf_92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461:SF69">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01697">
        <property type="entry name" value="Glyco_transf_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="436" mass="50331" checksum="55B4FBF31C56F1A3" modified="2019-05-08" version="1">MSCYGRIFFFVMVLFVLILYSLLYYWVTRNSLSSKSTGLIDLCQGEIARDTITALKYNKTFIIAPYFDKRQINLTRVIGIVHHNEVRDLYCWFCCQSDGKVSVERAAIDVHSDLFGFPYGTADLLCVEPQNCDPNYVSIHWSPKGNIVQLPRFEIKNRDDKTLSVEFTVCISTMFGNYNNVLQFIQSIEMYKILGAQKVVIYKNSCSQLMEKALEFYVAEGTVEIIPWPITSYFNVSSYWHFSMDTKDIGYYGQITALNDCVYRNMYRSKFVLLNDIDEIILPIKHLNWKTMMSSLQEQNPGTGIFLFENHIFPKTVYSSNEAFNISSWSAVPGVNILQHIYKEPDRPWAFSPRKMIVDPKKVIQTSVHSVLKAYGSSLEVSRDIAITYHCRDPLQGNIPKKFLTKDPTLWRYNLSLIKNVNKLTVCESNKTTVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I9Z5</accession>
    <name>A0A452I9Z5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ras GTPase-activating protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Ras p21 protein activator</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">p120GAP</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts with SQSTM1. Interacts with SPSB1; the interaction does not promote degradation. Interacts with CAV2 (tyrosine phosphorylated form). Directly interacts with NCK1. Interacts with PDGFRB (tyrosine phosphorylated). Interacts (via SH2 domain) with the 'Tyr-9' phosphorylated form of PDPK1. Interacts with tyrosine-phosphorylated EPHB4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I9Z5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024511"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027893.1">
        <property type="protein sequence ID" value="ENSGAGP00000024511.1"/>
        <property type="gene ID" value="ENSGAGG00000015920.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08400">
        <property type="entry name" value="C2_Ras_p21A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13260">
        <property type="entry name" value="PH_RASA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10354">
        <property type="entry name" value="SH2_Cterm_RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10353">
        <property type="entry name" value="SH2_Nterm_RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11788">
        <property type="entry name" value="SH3_RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000007">
        <property type="entry name" value="RAS p21 protein activator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000090">
        <property type="entry name" value="RAS p21 protein activator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000050">
        <property type="entry name" value="RAS p21 protein activator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000052">
        <property type="entry name" value="RAS p21 protein activator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000033">
        <property type="entry name" value="RAS p21 protein activator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000046">
        <property type="entry name" value="RAS p21 protein activator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035842">
        <property type="entry name" value="RasGAP_C_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035841">
        <property type="entry name" value="RasGAP_N_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035652">
        <property type="entry name" value="RasGAP_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF146">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 13" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 14" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH2" evidence="16">
        <location>
            <begin position="143"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="241"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="16">
        <location>
            <begin position="310"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="18">
        <location>
            <begin position="433"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="19">
        <location>
            <begin position="541"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="20">
        <location>
            <begin position="707"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="48"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="940" mass="105246" checksum="9A23653634F9347E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IB81</accession>
    <name>A0A452IB81_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spermatid perinuclear RNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IB81"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025001"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028461.1">
        <property type="protein sequence ID" value="ENSGAGP00000025001.1"/>
        <property type="gene ID" value="ENSGAGG00000018279.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19897">
        <property type="entry name" value="DSRM_STRBP-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19909">
        <property type="entry name" value="DSRM_STRBP_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.40:FF:000001">
        <property type="entry name" value="interleukin enhancer-binding factor 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000006">
        <property type="entry name" value="interleukin enhancer-binding factor 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000008">
        <property type="entry name" value="interleukin enhancer-binding factor 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000003">
        <property type="entry name" value="interleukin enhancer-binding factor 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006561">
        <property type="entry name" value="DZF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049402">
        <property type="entry name" value="DZF_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049401">
        <property type="entry name" value="DZF_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044472">
        <property type="entry name" value="STRBP_DSRM_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45762:SF1">
        <property type="entry name" value="SPERMATID PERINUCLEAR RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45762">
        <property type="entry name" value="ZINC FINGER RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20965">
        <property type="entry name" value="DZF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07528">
        <property type="entry name" value="DZF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00572">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51703">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="DZF" evidence="13">
        <location>
            <begin position="45"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="12">
        <location>
            <begin position="426"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="12">
        <location>
            <begin position="549"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="503"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="396"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="505"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="711" mass="76736" checksum="4766FC84C098A678" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBX0</accession>
    <name>A0A452IBX0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-hydroxyisobutyryl-CoA hydrolase</fullName>
            <shortName evidence="9">HIB-CoA hydrolase</shortName>
            <shortName evidence="9">HIBYL-CoA-H</shortName>
            <ecNumber evidence="9">3.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">3-hydroxyisobutyryl-coenzyme A hydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>3-hydroxy-2-methylpropanoyl-CoA + H2O = 3-hydroxy-2-methylpropanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20888"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57340"/>
            <dbReference type="EC" id="3.1.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid degradation; L-valine degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.4" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452IBX0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025188"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028677.1">
        <property type="protein sequence ID" value="ENSGAGP00000025188.1"/>
        <property type="gene ID" value="ENSGAGG00000018424.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00362"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003860">
        <property type="term" value="F:3-hydroxyisobutyryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000026">
        <property type="entry name" value="3-hydroxyisobutyryl-CoA hydrolase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045004">
        <property type="entry name" value="ECH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032259">
        <property type="entry name" value="HIBYL-CoA-H"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004127">
        <property type="entry name" value="PRK05617.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176:SF3">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16113">
        <property type="entry name" value="ECH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0101">Branched-chain amino acid catabolism</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl-CoA hydratase/isomerase" evidence="10">
        <location>
            <begin position="48"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="285" mass="31419" checksum="61EA4A27F632D55D" modified="2019-05-08" version="1">MEFYSILYGYSTLKTLFYLVSLFLRVQSMSKHTDSTAEVLLEKRGCAGVITLNRPKALNAFNLSMIRQIFLQLKTWEKDPETFLIIIKGTGGKAFCAGGDIRAITDAGKVGDRLTQDFFREEYILNNAIGTCQKPYVALIDGITMGGGVGLSVHGHFRVATEKTLFAMPETAIGLFPDVGGGYFLPRLSGKIGYCLALTGFRLKGRDSQKAGIATHFVESEKLPALEEDLIALKSPSKENVADVLNSYHMKVICWLATISNFNKGATIKLNNNLIYITIICCMFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IC68</accession>
    <name>A0A452IC68_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">TNF receptor-associated factor</fullName>
            <ecNumber evidence="13">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TNF receptor-associated factor family. A subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A452IC68"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025363"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028870.1">
        <property type="protein sequence ID" value="ENSGAGP00000025363.1"/>
        <property type="gene ID" value="ENSGAGG00000018548.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031996">
        <property type="term" value="F:thioesterase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16642">
        <property type="entry name" value="mRING-HC-C3HC3D_TRAF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000001">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000274">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000323">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000356">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012227">
        <property type="entry name" value="TNF_rcpt-assoc_TRAF_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049342">
        <property type="entry name" value="TRAF1-6_MATH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049440">
        <property type="entry name" value="TRAF3/5_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027130">
        <property type="entry name" value="TRAF5_C3HC3D_RING-HC_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF83">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21355">
        <property type="entry name" value="TRAF-mep_MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21363">
        <property type="entry name" value="TRAF3_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015614">
        <property type="entry name" value="TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9 13 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="44"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="18">
        <location>
            <begin position="127"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="18">
        <location>
            <begin position="182"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="17">
        <location>
            <begin position="402"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="14">
        <location>
            <begin position="127"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="14">
        <location>
            <begin position="182"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="242"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR015614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="557" mass="64417" checksum="8C41804CA6F5533D" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICS4</accession>
    <name>A0A452ICS4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ICS4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025304"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028806.1">
        <property type="protein sequence ID" value="ENSGAGP00000025304.1"/>
        <property type="gene ID" value="ENSGAGG00000018514.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008395">
        <property type="term" value="F:steroid hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008069">
        <property type="entry name" value="Cyt_P450_E_grp-I_CYP2D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF364">
        <property type="entry name" value="CYTOCHROME P450 2U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01686">
        <property type="entry name" value="EP450ICYP2D"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="318" mass="37008" checksum="0E7CCE361B75A925" modified="2019-05-08" version="1">MLRYGKDPFNPFPIISNSVSNVICSMAFGRRFDYEDIEFKTMLSLMSRALEISVNSHVLLVNICPWLYYLPFGPFRELRQIEFDVTAFLKKIIAQHRDTLDRENPRDFIDMYLLQVEEERESNNESSFNEDYLFFIIGDLFIAGTDTTTNTLLWCLLYMSLHSEVQEKVHTEIEVVIGRDRAPSLTDKAHMPFTEATIMEVQRMTVVVPLSVPRMASATAVLRGYTIPKGSVIVPNLWSVHRDPNIWERPDDFQPTRFLDENGQLIKREMFIPFGIGKISRYSWNGLFCLKFGVRVALPKSLWHLPFSYFVIENNIHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICZ2</accession>
    <name>A0A452ICZ2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphodiesterase</fullName>
            <ecNumber evidence="10">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.4.53"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452ICZ2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025377"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028885.1">
        <property type="protein sequence ID" value="ENSGAGP00000025377.1"/>
        <property type="gene ID" value="ENSGAGG00000018549.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000001">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="322"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="416"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="73"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="473" mass="53227" checksum="E31B5B91B4675212" modified="2019-05-08" version="1">TAMCLSQELEDTNKWGLDMFKITEYSGNHPLTVIMYSIFQERDLMKTFCIPADTFITYMMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEVMAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDVSVLENHHLAVGFKLLQEENCDIFQHLSKKQRQSLRKMAIDMVLATDMSKHMNLLADLKTMVETKKVTSLGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLELYRKWTDRIMVEFFHQGDREREKGMEISPMCDKLSASVEKSQVGFIDYIAHPLWETWADLVHPDAQEILDTLEDNREWYQSMIPHSPSLPEEQDPEKGGGGEKFQFQLTLEEEGESDTELEEAESPLEEDNSGSDSKTLATDDSESAELERLSPGSAGRDSPPPAHSSPLRNTLAKQKEVLHFDNRRALERTLSQPDGGTSLGCPELGRRGSLEARELGLDSEGNLTFLPLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IE41</accession>
    <name>A0A452IE41_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Collagen IV NC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
            <location evidence="2">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000026161"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029756.1">
        <property type="protein sequence ID" value="ENSGAGP00000026161.1"/>
        <property type="gene ID" value="ENSGAGG00000019109.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.240.10:FF:000001">
        <property type="entry name" value="Collagen IV alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.240.10">
        <property type="entry name" value="Collagen IV, non-collagenous"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001442">
        <property type="entry name" value="Collagen_IV_NC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036954">
        <property type="entry name" value="Collagen_IV_NC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1112">
        <property type="entry name" value="COL_CUTICLE_N DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01413">
        <property type="entry name" value="C4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00111">
        <property type="entry name" value="C4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51403">
        <property type="entry name" value="NC1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019224434" description="Collagen IV NC1 domain-containing protein" evidence="10">
        <location>
            <begin position="27"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="domain" description="Collagen IV NC1" evidence="11">
        <location>
            <begin position="1450"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="172"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="324"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="960"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="172"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="266"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="468"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="541"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="551"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="573"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="659"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="670"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="864"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="890"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="913"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1009"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1019"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1153"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1290"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1301"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1324"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1344"/>
            <end position="1358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1665" mass="162210" checksum="A9BAB370147F38A7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IEP3</accession>
    <name>A0A452IEP3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxiredoxin-5</fullName>
            <ecNumber evidence="10">1.11.1.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452IEP3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026297"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029897.1">
        <property type="protein sequence ID" value="ENSGAGP00000026297.1"/>
        <property type="gene ID" value="ENSGAGG00000019176.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03013">
        <property type="entry name" value="PRX5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000020">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430:SF16">
        <property type="entry name" value="PEROXIREDOXIN-5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 10" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7 10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="55"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="9">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR637944-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="214" mass="22405" checksum="71EBD7782E4DB62A" modified="2019-05-08" version="1">MATVIQAVSTVTAWLGVAGLVLRRGRGLLRAPPLPGSKPRGTRSFGVRAAAMAPVKVGDKLPSVEVYEGDPGTKVDVATLFKGKKGILFGVPGAFTPGCSKTHLPGYVEQAGALKAKGVELIACLAVNDVFVMSEWGKAHGAQGKVRMLADPTGAFGKATELLLDKETLRDLFGTNRCKRFSMVLENGVVKALNVEEDGTGLSCSLANNILSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IGW4</accession>
    <name>A0A452IGW4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Neurexin 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IGW4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027118"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030811.1">
        <property type="protein sequence ID" value="ENSGAGP00000027118.1"/>
        <property type="gene ID" value="ENSGAGG00000019725.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000014">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF49">
        <property type="entry name" value="AXOTACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019529532" description="Neurexin 2" evidence="3">
        <location>
            <begin position="27"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="4">
        <location>
            <begin position="28"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="200"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="31381" checksum="7E1DA4F796C59F6E" modified="2019-05-08" version="1">MRLGAMARLLPLVLLMGALLGPGAGGAALEFGGTTGQWARYARWDASSLGELSFSLKTNVSRALVLYLDDGGNCDFLELMIADGRLRLRFTISCAEPAKLHLETPVDDDRWHMVLLTRNYRETMLVVDGEAKVAEVKSKRRDMAVESDLFLGGIPPDVRLSALTLSTVKYEVPFKGLVANLKVGDTPPALLGSQGIRSDTEYLCTKQNPCAHGGLCSVLNGEVQCDCSMTGFQGKFCSEGENFLGKLQHLVYGSPYKSGFPYLVWEPEPLERPMHSQRGGLHPSVTCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIA4</accession>
    <name>A0A452IIA4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Nuclear pore complex protein Nup50</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">50 kDa nucleoporin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Nuclear pore-associated protein 60 kDa-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Nucleoporin Nup50</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with regulatory proteins of cell cycle progression including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IIA4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027565"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031316.1">
        <property type="protein sequence ID" value="ENSGAGP00000027565.1"/>
        <property type="gene ID" value="ENSGAGG00000020029.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13170">
        <property type="entry name" value="RanBD_NUP50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000179">
        <property type="entry name" value="Nuclear pore complex protein Nup50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015007">
        <property type="entry name" value="NUP2/50/61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF141">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08911">
        <property type="entry name" value="NUP50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="12" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RanBD1" evidence="21">
        <location>
            <begin position="357"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="118"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="243"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="327"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00081812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="472" mass="50646" checksum="C1A69B9EA119528C" modified="2019-05-08" version="1">MAKRIAEKELTDRNWDQEDETEEVGTFSVASEEVLKNRAIKKAKRRNVGVESDNGGAFKGFKGFVLPSGKGGGGFTGFGNGTEMKPLGGLSNGSSGISSTPSFTSVKTVSEVQPAFVGSVTSNGPTTTLVEKKASGTKVNGDSQQSSSSGYTPNKMCSSSVYHKQLAALNCSVRDWIVKHVNTNPLCDLTPIFRDYEKYLASIEQHHGSSSDSGSENESNKTTGTQSVPTFGTLKLQQGSTFLFNSNKTEDESDKKTESTSEKNRESPLGAASNVSFNFGKNVDSSILGSLGSGTLTSFSFSPGNSGVFGKDAKQGKSVSTLSTRVLEAQTESGSSEDKGGDEEDEEPPKTVVNEVKEDDAFYSKKCKLFYKKDNEFKEKGVGTLHLKPAGNEKTQLLVRADTNLGNILLNVLVPPKMPCTRTGKNNVLIVCVPNPPIDEKNSTVPVTMLIRVKTSEDADELHKILLEKKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJ76</accession>
    <name>A0A452IJ76_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452IJ76"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027918"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031709.1">
        <property type="protein sequence ID" value="ENSGAGP00000027918.1"/>
        <property type="gene ID" value="ENSGAGG00000020272.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060631">
        <property type="term" value="P:regulation of meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000115">
        <property type="entry name" value="Wee1-like kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF75">
        <property type="entry name" value="WEE1-LIKE PROTEIN KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019358029" description="non-specific protein-tyrosine kinase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="42"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="380" mass="42248" checksum="F3B854B4AD6D2C4F" modified="2019-05-08" version="1">SAKGLLLSVSMLAWCLDWCLQYFFKRFLLRESNMVSRYKKEFLELEKIGVGEFGSVYKCIKRLDGCVYAVKRSKRPLAGSSDEQMALREVYAHAVLGHHPHVVRYYSAWAEDDHMIIQNEHCNGGSLQDALLENAKTGHYFQEAELKEILLQVSMGLKYIHSSGLAHMDIKPSKCTHSGTSPRKVDLWASVAFLVSFLLLLGDLGHVTSITNPQVEEGDSRFLANEILQEQYCYLPKADIFALALTMALAAGSGPLPCNDAMWHHIRRGNLPPIPQQLTHGFHGLLKLMVHPDPVARPSATALTKHPILRPSLGKAAQLQKQLNVEKFKTAICQLMCQDYFCPSFSCLLGTPALCLAEPDTSICSNTDPGSELLAPKLQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILQ8</accession>
    <name>A0A452ILQ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DAZ-associated protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Deleted in azoospermia-associated protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proline-rich transcript in brain protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">In unstressed cells, promotes SIAH1-mediated polyubiquitination and degradation of the serine/threonine-protein kinase HIPK2, probably by acting as a loading factor that potentiates complex formation between HIPK2 and ubiquitin ligase SIAH1. In response to DNA damage, localizes to the nucleus following phosphorylation by HIPK2 and modulates the expression of a subset of TP53/p53 target genes by binding to TP53 at target gene promoters. This limits the expression of a number of cell death-mediating TP53 target genes, reducing DNA damage-induced cell death. Enhances the binding of transcription factor TCF7L2/TCF4, a Wnt signaling pathway effector, to the promoters of target genes. Plays a role in stress granule formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILQ8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032687.1">
        <property type="protein sequence ID" value="ENSGAGP00000028772.1"/>
        <property type="gene ID" value="ENSGAGG00000020852.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022730">
        <property type="entry name" value="DAZ_assoc-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31638">
        <property type="entry name" value="DAZ-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31638:SF3">
        <property type="entry name" value="DAZ-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11029">
        <property type="entry name" value="DAZAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="168" mass="17239" checksum="F30ABA815065B857" modified="2019-05-08" version="1">MNSKGQYPTQPSYPVQPPGNPSVYPQTMPLPQAPPYTDAPPAYSELYRPSFVHPGAATVPTMSAAYPGTSLYLPMAQSVAVGPMGSSVPMAYYPVGPVYPPGSTVLVEGGFDAGARFGAGATGSVPPPPPGCPPNAAQLAAMQGANVLLTQRKGSYFMGGSDGGYTIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IN17</accession>
    <name>A0A452IN17_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein FAM118B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in Cajal bodies formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nuclear body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM118 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IN17"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029250"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033220.1">
        <property type="protein sequence ID" value="ENSGAGP00000029247.1"/>
        <property type="gene ID" value="ENSGAGG00000021159.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000033221.1">
        <property type="protein sequence ID" value="ENSGAGP00000029248.1"/>
        <property type="gene ID" value="ENSGAGG00000021159.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000033222.1">
        <property type="protein sequence ID" value="ENSGAGP00000029250.1"/>
        <property type="gene ID" value="ENSGAGG00000021159.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038916">
        <property type="entry name" value="FAM118"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28623">
        <property type="entry name" value="PROTEIN FAM118B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28623:SF1">
        <property type="entry name" value="PROTEIN FAM118B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13289">
        <property type="entry name" value="SIR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="322"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="355" mass="39973" checksum="48D00CD85716430A" modified="2019-05-08" version="1">MASTVSVNLDKDDTFDGIPPVKKPRKLLPSLKTKKPQELVLVIGTGISAAVAPQVPALKSWKGLIQALLDAAIDFDLLEEEESRKFQKGLQEDKNLVHVAHDLIQKLSPRTSNVRSTFFKDCLYEVFDDLESKMEDSGKQLLQSVLRLMENGALVLTTNFDNLLELYAADQGKQLESLDLTDEKKVLEWAQEKRKLSVLHIHGVYTNPSGIVLHPAGYQNVLRNTEVMGEIQKLYENKSFLFLGCGRTVDDTTFQALFLEAVKRKSDLEHFMLVQRGDVDEFKKLRENMLDKGIKVISYGNEYADLPDYFERLTSEISMRDQTGLPREGQLNGSSSAHTEIKEGAAPEHLPLQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452INJ5</accession>
    <name>A0A452INJ5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CCN family member 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cellular communication network factor 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Connective tissue growth factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Is involved in the stimulation of osteoblast differentiation and has a critical role in osteogenesis. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Monomer. Interacts with TSKU.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452INJ5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029435"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033425.1">
        <property type="protein sequence ID" value="ENSGAGP00000029435.1"/>
        <property type="gene ID" value="ENSGAGG00000021272.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000036">
        <property type="entry name" value="Connective tissue growth factor (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000015">
        <property type="entry name" value="CYR61 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050941">
        <property type="entry name" value="CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017891">
        <property type="entry name" value="Insulin_GF-bd_Cys-rich_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043973">
        <property type="entry name" value="TSP1_CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348:SF7">
        <property type="entry name" value="CCN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348">
        <property type="entry name" value="CONNECTIVE TISSUE GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19035">
        <property type="entry name" value="TSP1_CCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01185">
        <property type="entry name" value="CTCK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00222">
        <property type="entry name" value="IGFBP_N_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="6" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="IGFBP N-terminal" evidence="19">
        <location>
            <begin position="160"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="18">
        <location>
            <begin position="236"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="17">
        <location>
            <begin position="392"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="77"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="485" mass="52791" checksum="731F08B2D7687528" modified="2019-05-08" version="1">MPLDEGYTALFLCAEVCQLLQTGGMLSVKGSGSIRCELMRQEGGEECEECRCLCRTPFSRLASPGRLECIKAARLPASDPENNSPARETGPSRDKALGQQAGNPARHRSPPAQTPSARSTDTQAEEPLHRLLPLPSMAARMGPSSFAVALLVALLCWEVSGQDCSGQCQCGAGPPPTCPAGVSLVQDGCGCCRVCAKQLGELCTERDPCDHHKGLFCDFGSPANRRIGVCTARDGAPCVFGGMVYRSGESFQSSCKYQCTCLDGAVGCVPLCSMDVRLPSPDCPSPRRIKLPGKCCEEWVCDELREQTAVGPALAAYRPEDTYGPDQAMMMRANCLVQTTEWSACSKTCGMGISTRVTNDNAFCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKISKPIKFELSGCTSVKTYRAKFCGVCTDGRCCTPHRTATLPVEFKCPDGEVMKRRMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IP38</accession>
    <name>A0A452IP38_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IP38"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029650"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033666.1">
        <property type="protein sequence ID" value="ENSGAGP00000029650.1"/>
        <property type="gene ID" value="ENSGAGG00000021406.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015057">
        <property type="term" value="F:thrombin-activated receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15371">
        <property type="entry name" value="7tmA_PAR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000040">
        <property type="entry name" value="Coagulation factor 2 (thrombin) receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003943">
        <property type="entry name" value="Prot_act_rcpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003912">
        <property type="entry name" value="Protea_act_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF0">
        <property type="entry name" value="PROTEINASE-ACTIVATED RECEPTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01428">
        <property type="entry name" value="PROTEASEAR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01429">
        <property type="entry name" value="PROTEASEAR3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019077751" description="G-protein coupled receptors family 1 profile domain-containing protein" evidence="13">
        <location>
            <begin position="18"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="263"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="114"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="169"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR603912-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="384" mass="44351" checksum="A62D94EF476A92B6" modified="2019-05-08" version="1">MKTLVLIITGLLSLSSSLRQRDSKHGDNSSASQDVPDIKTFRGMPQGIYDYIPPSDIEGWTKTVHNKERKCSSGKSNGSILKVNNTTMEYLSSSLSTKLVPATYILVVLLGVPSNAITLWMLFFRIRSVCTAIFYTNLAISDFLFCIILPFKIAYHLNGNNWIFGEIMCRTMTAIFYGNMYCSILLLMCISISRYVAIVHPFTYRSLPKRTYSTLACGIVWTIVFLYMLPFCIMKQSYYLEQLNISTCHDVHNACETLSLFQFYYFISLAIFGFVIPLTIIIFCYISIIQTLNTYERKWFWYVKISLLILMIFAICFTPSNIILIVHHVNYYYNKTDGLYFFYLIALSLSSLNSCLDPFLYYLMSKIREQSNVYLTMVKISREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPU5</accession>
    <name>A0A452IPU5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IPU5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029929"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033988.1">
        <property type="protein sequence ID" value="ENSGAGP00000029929.1"/>
        <property type="gene ID" value="ENSGAGG00000021604.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08616">
        <property type="entry name" value="PI-PLCXD1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042158">
        <property type="entry name" value="PLCXD1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF24">
        <property type="entry name" value="PI-PLC X DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26146">
        <property type="entry name" value="PI-PLC_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="332" mass="38523" checksum="73429FB47E0380CE" modified="2019-05-08" version="1">MEATSKRSSHLCHENGDWMSHLPENLWDVPLHNLSLPGSHDAMTYCLDKNSDVSVNESKLLQVIDKCMPCIIRPIIMKWSITQVLSVSEQLDAGIRYLDFRIAHKPNDSSVNLYFVHMVYTTAVVEDILREILKWLQTHPQEVVILACRNFDGLTKELHHHLISCIKKIFHSKLCPKDVVPTLRNMWTYRYQVIISYEDVTEVMNHRELWPAIPYWWGNKTAARDLIQYLERMKKNGRPDCFFVAGINLTSDLEYILAHPFGSVKKMALSSLPYLKLWIKKQYPGPKRECINIIAGDFIGNYDFVNDVIELNKKIIPPLHYDGNETELKDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQ70</accession>
    <name>A0A452IQ70_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IQ70"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029993"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034062.1">
        <property type="protein sequence ID" value="ENSGAGP00000029993.1"/>
        <property type="gene ID" value="ENSGAGG00000021654.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000113">
        <property type="entry name" value="homeobox protein Hox-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046327">
        <property type="entry name" value="HXA1/B1/D1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946:SF5">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946">
        <property type="entry name" value="HOMEOBOX PROTEIN ROUGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="214"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="216"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="61"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="267"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="295"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="312" mass="33885" checksum="0AA48D3B1448004C" modified="2019-05-08" version="1">MDNTRMNSFLEYAICNRGTSAYSPKGYHHLEQGATSFPACSGTPSSDSYNGDGRFVVAGSATVPSHPAQPNPGYHHPHPSGLGLPFSGSASAGYPPQACNPSYGHHQFYLSQQETDGVYFQSAPAGSNLSSGSDGYCSAVSGSGQYQHHPYGQEQQGYLQGAYSHLSAALNEDKDHACPSELCPTASLTQTFDWMKVKRNPPKTAKVSEYGLVGPPNAIRTNFTTKQLTELEKEFHFNKYLTRARRVEIAATLELNETQVKIWFQNRRMKQKKREKEGLAPAPAPRAAKEVGEASDQSNFTSPEASPNSVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IS27</accession>
    <name>A0A452IS27_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000030817"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034949.1">
        <property type="protein sequence ID" value="ENSGAGP00000030817.1"/>
        <property type="gene ID" value="ENSGAGG00000022156.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000034952.1">
        <property type="protein sequence ID" value="ENSGAGP00000030820.1"/>
        <property type="gene ID" value="ENSGAGG00000022156.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000034955.1">
        <property type="protein sequence ID" value="ENSGAGP00000030823.1"/>
        <property type="gene ID" value="ENSGAGG00000022156.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd20951">
        <property type="entry name" value="IgI_titin_I1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000069">
        <property type="entry name" value="Alpha-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000029">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000124">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000134">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000192">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000197">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000222">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000233">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002172">
        <property type="entry name" value="Myomesin 1a (skelemin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000179">
        <property type="entry name" value="Myomesin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000670">
        <property type="entry name" value="Myomesin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF22">
        <property type="entry name" value="MYOMESIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="143"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="280"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="374"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="502"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="603"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="699"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="804"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="1119"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="1347"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1431"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1450"/>
            <end position="1460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1469" mass="165320" checksum="DAEA3418E5A82D51" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IS47</accession>
    <name>A0A452IS47_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Dynein axonemal heavy chain 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000030834"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034968.1">
        <property type="protein sequence ID" value="ENSGAGP00000030834.1"/>
        <property type="gene ID" value="ENSGAGG00000022166.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000034977.1">
        <property type="protein sequence ID" value="ENSGAGP00000030841.1"/>
        <property type="gene ID" value="ENSGAGG00000022166.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097729">
        <property type="term" value="C:9+2 motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000002">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000219">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000682">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000007">
        <property type="entry name" value="Dynein axonemal heavy chain 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002141">
        <property type="entry name" value="Dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000002">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000411">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000002">
        <property type="entry name" value="Dynein heavy chain 9, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000002">
        <property type="entry name" value="Dynein heavy chain 9, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000049">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.130:FF:000001">
        <property type="entry name" value="Dynein, axonemal, heavy chain 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF4">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain tail" evidence="16">
        <location>
            <begin position="52"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain linker" evidence="17">
        <location>
            <begin position="1130"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="18">
        <location>
            <begin position="1670"/>
            <end position="1996"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA 5 extension" evidence="22">
        <location>
            <begin position="2154"/>
            <end position="2271"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain 3 AAA+ lid" evidence="23">
        <location>
            <begin position="2489"/>
            <end position="2587"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="20">
        <location>
            <begin position="2633"/>
            <end position="2892"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="19">
        <location>
            <begin position="2905"/>
            <end position="3248"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="21">
        <location>
            <begin position="3275"/>
            <end position="3492"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="15">
        <location>
            <begin position="3733"/>
            <end position="3853"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="24">
        <location>
            <begin position="3885"/>
            <end position="4021"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="25">
        <location>
            <begin position="4027"/>
            <end position="4322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="628"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="2897"/>
            <end position="2983"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3120"/>
            <end position="3168"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3482"/>
            <end position="3512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF17852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF17857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="28">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="4324" mass="495922" checksum="14492D7B283617A2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IS88</accession>
    <name>A0A452IS88_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Costars family protein ABRACL</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">ABRA C-terminal-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the costars family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IS88"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035023.1">
        <property type="protein sequence ID" value="ENSGAGP00000030882.1"/>
        <property type="gene ID" value="ENSGAGG00000022209.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032970">
        <property type="term" value="P:regulation of actin filament-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1540:FF:000002">
        <property type="entry name" value="costars family protein ABRACL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1540">
        <property type="entry name" value="Costar domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044302">
        <property type="entry name" value="Costars"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027817">
        <property type="entry name" value="Costars_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038095">
        <property type="entry name" value="Costars_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46334">
        <property type="entry name" value="COSTARS FAMILY PROTEIN ABRACL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46334:SF1">
        <property type="entry name" value="COSTARS FAMILY PROTEIN ABRACL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14705">
        <property type="entry name" value="Costars"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01283">
        <property type="entry name" value="Costars"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Costars" evidence="4">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00031406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="81" mass="9018" checksum="DD02EF646B0FEF82" modified="2019-05-08" version="1">MNVEHEINLLVEEIRRLGTKNADGKLSVKFGVLFADEKCANLFEALVGTLKAAKQRKIVTYPGELLLQGVHDNVDILLLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITU1</accession>
    <name>A0A452ITU1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">E3 ubiquitin-protein ligase RNF220</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">RING finger protein 220</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">RING-type E3 ubiquitin transferase RNF220</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with SIN3B. Interacts with CTNNB1 (via Armadillo repeats 2-8). Interacts with USP7 (via MATH domain).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452ITU1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031460"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035682.1">
        <property type="protein sequence ID" value="ENSGAGP00000031460.1"/>
        <property type="gene ID" value="ENSGAGG00000022596.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16563">
        <property type="entry name" value="RING-HC_RNF220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000195">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052443">
        <property type="entry name" value="E3_ubiq-ligase_RNF220-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031824">
        <property type="entry name" value="RNF220_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040178">
        <property type="entry name" value="RNF220_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13459:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13459">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF220 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15926">
        <property type="entry name" value="RNF220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="21">
        <location>
            <begin position="299"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="14"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="351" mass="39479" checksum="A8797783F05C591F" modified="2019-05-08" version="1">MEQELEKLTQLNVSKNSILKDSMAPGTPKSILLSASIKREGDSRTASPHSSDDIHHSDRYQTFLRVRANRQTRLNARIGKMKRRKQDEGQVCPLCSRPLSGSEEEMSRHVEQCLAKREGSCMTEDDSVDIENENGNRFEEYEWCGQKRIRATTLLEGGFRGSGFIMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADIIPCTGEEPGEAKEREALRGAVLNGGTPSTRITPEFSKWASDEMPSTSNGESSKQEALQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYTMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITSPGDLRRIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IUW1</accession>
    <name>A0A452IUW1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Inflammasome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NLRP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IUW1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031912"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036191.1">
        <property type="protein sequence ID" value="ENSGAGP00000031912.1"/>
        <property type="gene ID" value="ENSGAGG00000022906.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050637">
        <property type="entry name" value="NLRP_innate_immun_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45690">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45690:SF19">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1271">Inflammasome</keyword>
    <keyword evidence="8" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="NACHT" evidence="10">
        <location>
            <begin position="85"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="988" mass="113638" checksum="97D0846927DE1CD6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IV66</accession>
    <name>A0A452IV66_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Centrosomal protein of 97 kDa</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucine-rich repeat and IQ domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a key negative regulator of ciliogenesis in collaboration with CCP110 by capping the mother centriole thereby preventing cilia formation. Required for recruitment of CCP110 to the centrosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IV66"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031771"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036030.1">
        <property type="protein sequence ID" value="ENSGAGP00000031771.1"/>
        <property type="gene ID" value="ENSGAGG00000022821.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902018">
        <property type="term" value="P:negative regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000165">
        <property type="entry name" value="Centrosomal protein of 97 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000199">
        <property type="entry name" value="centrosomal protein of 97 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF2">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 97 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="473"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="704"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="790"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="592"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="715"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="795"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="874" mass="96880" checksum="BA8F4D96FB85C0E5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWA4</accession>
    <name>A0A452IWA4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myb/SANT-like DNA-binding domain-containing protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IWA4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032177"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036479.1">
        <property type="protein sequence ID" value="ENSGAGP00000032177.1"/>
        <property type="gene ID" value="ENSGAGG00000023032.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000036480.1">
        <property type="protein sequence ID" value="ENSGAGP00000032178.1"/>
        <property type="gene ID" value="ENSGAGG00000023032.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026162">
        <property type="entry name" value="MSANTD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028002">
        <property type="entry name" value="Myb_DNA-bind_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21732">
        <property type="entry name" value="MYB/SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21732:SF0">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13873">
        <property type="entry name" value="Myb_DNA-bind_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="4">
        <location>
            <begin position="11"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="206"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00021375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="347" mass="41422" checksum="8C34FF4D700C077B" modified="2019-05-08" version="1">MEKMKQLKRKRKSNFSVQETQTLLKEIRKRKEVLFSKQLNTTINEMKRKAWEEIAECVNAVGEGEQRTGTEVKRRYLDWRALMKRKRLNANIKLVGAGFHLPSSDLDDSVNEEMDEKMGFTNENSFEWLSITDFREASGSLTEIKVEEEEDPQNFEFPIEEEEEILSSVLPDSKKESDLPDFTHIEEFGNLSSAQARLAYEDSHLLINLEKQKLELEKQRLDIEAERLQVEKERLQIEKERLRHVSLEHERLQLEKERLQIEREKLRLEALRAEKPALENDITQVEKPILQPLDLETEKLKLEKERLQLEKERLQFLKYESEKLQIEKERLQVEKERFRIQREGHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYM9</accession>
    <name>A0A452IYM9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the UQCC4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYM9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033195"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037619.1">
        <property type="protein sequence ID" value="ENSGAGP00000033195.1"/>
        <property type="gene ID" value="ENSGAGG00000023672.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029160">
        <property type="entry name" value="UQCC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023248">
        <property type="entry name" value="UQCC4_vert"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35268">
        <property type="entry name" value="PROTEIN CCSMST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35268:SF1">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX ASSEMBLY FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15013">
        <property type="entry name" value="CCSMST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02042">
        <property type="entry name" value="CCSMST1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="145" mass="16204" checksum="F861A450B6C87861" modified="2019-05-08" version="1">ANRVLCALCKTGLRLALLRVYSSFFPSRPRLLSCPFCAVRRLSRHPGPEDVAESASSRPIPFSTSKASPRVWSVDRSMGSDYRRPWVKVLPVSLLGIGLLLWCVFREEAEVDKQLGAVFHQQVPGLLDETPENKILPPSSQQEKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1Y7</accession>
    <name>A0A452J1Y7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J1Y7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034419"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038969.1">
        <property type="protein sequence ID" value="ENSGAGP00000034419.1"/>
        <property type="gene ID" value="ENSGAGG00000024460.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001872">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002190">
        <property type="entry name" value="Zinc finger protein 658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000176">
        <property type="entry name" value="zinc finger protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000642">
        <property type="entry name" value="Zinc finger with KRAB and SCAN domains 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF73">
        <property type="entry name" value="ZINC FINGER PROTEIN 572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="10"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="67"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="411" mass="46925" checksum="0BF4048B762109AC" modified="2019-05-08" version="1">SLQASFPMPVTFEEVAVYFTQGQGALLDPAQRALYRDVMQENYKMVTSLAGFPIPKPDLIAQLERGEEPWVPDLQADEERESPRGTRTGEESRKLTQKHPGSEGKSWDSQNVLRNLVTCWRNHSSEKPYKCLDCGKTFSKNSDLIKHRRMHTGERPYKCLDCGKTFTQSSHLITHQRLHTGERPYKCLECGKGFNVRSNLIKHQIHTGERPHKCLDCGKSFSHSSHLTKHLRIHTGERPYKCLECGKTFNQSSHLIMHQRTHTGERHYKCLECGKSFVRRSALITHQRIHTGEKPHICLDCGKSFSQRSALIRHQRIHTGEKPHTCLNCGKRFIQRSHLITHQTIHKGEKTHKCLDCGKTSYFSSCPSPSLQPLALLPEYASGSPIDSGFQLLIPAFHPWLCDGCREQICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J437</accession>
    <name>A0A452J437_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zinc transporter ZIP12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Solute carrier family 39 member 12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Zrt- and Irt-like protein 12</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Zn(2+)(in) = Zn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29351"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J437"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035200"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039858.1">
        <property type="protein sequence ID" value="ENSGAGP00000035200.1"/>
        <property type="gene ID" value="ENSGAGG00000025020.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140410">
        <property type="term" value="F:monoatomic cation:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071578">
        <property type="term" value="P:zinc ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049406">
        <property type="entry name" value="ZIP4_12_EF-hand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041137">
        <property type="entry name" value="ZIP4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050799">
        <property type="entry name" value="ZIP_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191:SF4">
        <property type="entry name" value="ZINC TRANSPORTER ZIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21116">
        <property type="entry name" value="EF-hand_Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18292">
        <property type="entry name" value="ZIP4_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019548832" description="Zinc transporter ZIP12" evidence="12">
        <location>
            <begin position="25"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="381"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="613"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="644"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="674"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc transporter ZIP4 N-terminal" evidence="13">
        <location>
            <begin position="67"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc transporter ZIP4/12 EF-hand" evidence="14">
        <location>
            <begin position="234"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="505"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="505"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF18292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF21116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="703" mass="78442" checksum="9C98E730AA022E43" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J632</accession>
    <name>A0A452J632_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein Mdm4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MDM2/MDM4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J632"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035955"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040696.1">
        <property type="protein sequence ID" value="ENSGAGP00000035954.1"/>
        <property type="gene ID" value="ENSGAGG00000025497.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000040697.1">
        <property type="protein sequence ID" value="ENSGAGP00000035955.1"/>
        <property type="gene ID" value="ENSGAGG00000025497.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17673">
        <property type="entry name" value="MDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16784">
        <property type="entry name" value="mRING-HC-C2H2C4_MDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015458">
        <property type="entry name" value="MDM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016495">
        <property type="entry name" value="p53_neg-reg_MDM_2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46858">
        <property type="entry name" value="OS05G0521000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46858:SF12">
        <property type="entry name" value="PROTEIN MDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500699">
        <property type="entry name" value="MDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006748">
        <property type="entry name" value="p53_MDM_2/4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DM2" evidence="11">
        <location>
            <begin position="24"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="10">
        <location>
            <begin position="292"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="121"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR006748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="477" mass="53439" checksum="B2F64E90A710008C" modified="2019-05-08" version="1">MTSSTSEQHPASENACRIALGQANQVQPKLPLLKILQAAGAHGEIFTLKEVMHYLGQYIMVRQLYDKRQQHMVYCGGDQLGELLGLESFSVKDPSPVYDMLKRNLTSVTITDAAQTLALAKDQSVDNPSQDQLKTSTEGSSDTEGTEDKSGAPALSTSQRSYGNCEDKDLRENLSKSKKPKLDLVFEEWDVAGLPWWFLGNLRNNYKSRSNGSTDIQTNQDIDTAIVSDTTDDLWFLNESASDQYNVAVKVETVDCEEVGKESDKKVIEVTCTDDLEDSQSLSDDTDVEAASEDCWQCTKCKKFNSPVKRYCYRCWALRKDWYSDCPKLAHSLSLSTIDTIQGKKDDEGIDVPDCRRTVSAPVCRPKDLHTGECESHVDPGSSMESLGLTQECKVQEPQMHFGERKKEEVVEWQENIKNLLNPCLLCQKRPRDGNIIHGRSGHLVACFRCAKMLKKGKLPCPVCKKPIQMVIRIFMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J751</accession>
    <name>A0A452J751_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Protein turtle homolog B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Immunoglobulin superfamily member 9B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Transmembrane protein which is abundantly expressed in interneurons, where it may regulate inhibitory synapse development. May mediate homophilic cell adhesion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Found in a complex with MAGI2 and NLGN2, where it interacts with MAGI2 (via PDZ 5 and PDZ 6 domains).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the immunoglobulin superfamily. Turtle family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000036334"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041139.1">
        <property type="protein sequence ID" value="ENSGAGP00000036334.1"/>
        <property type="gene ID" value="ENSGAGG00000025752.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098982">
        <property type="term" value="C:GABA-ergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000272">
        <property type="entry name" value="Immunoglobulin superfamily member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000323">
        <property type="entry name" value="Immunoglobulin superfamily member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000389">
        <property type="entry name" value="Immunoglobulin superfamily member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000531">
        <property type="entry name" value="Immunoglobulin superfamily member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000226">
        <property type="entry name" value="protein turtle homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000245">
        <property type="entry name" value="protein turtle homolog B isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000321">
        <property type="entry name" value="protein turtle homolog B isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF240">
        <property type="entry name" value="PROTEIN TURTLE HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 25" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019096449" description="Protein turtle homolog B" evidence="25">
        <location>
            <begin position="25"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="728"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="26">
        <location>
            <begin position="43"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="26">
        <location>
            <begin position="144"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="26">
        <location>
            <begin position="233"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="26">
        <location>
            <begin position="329"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="26">
        <location>
            <begin position="425"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="27">
        <location>
            <begin position="517"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="27">
        <location>
            <begin position="619"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="763"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="992"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1100"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="992"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1020"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1113"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1131"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1156"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1201"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1250"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1279"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1318"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="1348"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1373"/>
            <end position="1382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00037805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00062279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00069171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00075032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1441" mass="158612" checksum="BDAE789E69F59736" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GEU2</accession>
    <name>A0A452GEU2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secretory carrier-associated membrane protein</fullName>
            <shortName evidence="5">Secretory carrier membrane protein</shortName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SCAMP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GEU2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000016"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000017.1">
        <property type="protein sequence ID" value="ENSGAGP00000000016.1"/>
        <property type="gene ID" value="ENSGAGG00000000008.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007273">
        <property type="entry name" value="SCAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687:SF8">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04144">
        <property type="entry name" value="SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="79"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="342" mass="38111" checksum="F15A5A3866BA235C" modified="2019-05-08" version="1">MSDFDSNPFADPDLNNPFKDPSVTQVTRNVPPGLDEYNPFSDSRTPPPGNVKIPNAPAPSTQPAIMKPTEEPPAYTQIAKEHALAQAELLKRQEELERKAAELDRREREMQNLNRQGGRKNNWPPLPGNFPVGPCFYQDFSVDIPVEFQKTVKIMYYLWMFHAVTLFLNIFGCLAWFCVAPTARGVDFGLSILWFLLFTPCSFVCWYRPLYGAFRSDSSFRFFVFFFVYICQFAVHVLQAAGFQGWGNCGWISSLTGLNESIPVGIMMIIITALFTASAVISLVMFKKVHGLYRTTGASFEKAQQEFATGVMSNKTVQTAAANAASTAATSAAQNAFKGNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFI2</accession>
    <name>A0A452GFI2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GFI2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000323"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000366.1">
        <property type="protein sequence ID" value="ENSGAGP00000000323.1"/>
        <property type="gene ID" value="ENSGAGG00000000244.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000038">
        <property type="entry name" value="Putative sodium-coupled neutral amino acid transporter 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF226">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="9">
        <location>
            <begin position="27"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="403" mass="44218" checksum="8921B4E9F1882843" modified="2019-05-08" version="1">MEELARESIGLLAKPTLESGSPNLSSAGAVFIMLKSSLGAGLLNFPWAFNKAGGITTAVLVELGSLIFLISGLVILGYASSISTQSTYQGVVREICGSAIGKLCEICFILNLFMISIAFLRVVGDQLEKLCDSLYMNMTLSGEGPPPSQHWYTDYRFTLTALCALVIFPLSVPREIGFQKYTSILGTLAACYLTLVIIIKYYLRTEQVIMHEVSSLASMFSVIPTICFGFQCHEACIAIYSSMHNKKLSLWIVVSVLSMLFCLLIYSLTGLYGYLTFGADVAADILMSYPGNDLIIIIARLLFGISIITIYPIVLLLGRSVLQDVCLNDKCRSVMLLEPYERCTRIVLTVTWIIVTLVIALFVPDISEVISIIGGISAFFIFIFPGLCLVCAMETEPIEPRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFN2</accession>
    <name>A0A452GFN2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Interacts with the GAP domain of NF1. Interacts (via TPR repeats) with HSP90AA1 and HSPA8.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GFN2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000359"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000403.1">
        <property type="protein sequence ID" value="ENSGAGP00000000359.1"/>
        <property type="gene ID" value="ENSGAGG00000000309.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000367">
        <property type="entry name" value="Tetratricopeptide repeat domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052769">
        <property type="entry name" value="TPR_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46014">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46014:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="191"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="57"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="101"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00063969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="295" mass="33844" checksum="659F352DD91E39A9" modified="2019-05-08" version="1">MEETEKSSPPEELLHGLKLSDQKSSESTGPLTAGSKDAHHPRSLDRGDLHSSTQEPRAADECFHDCNDSFEVKEPGMEQERDNREDLARKQTELDEEYLLELEKNMPEEEKQKRRKESTKLKEKGNEQFKKGEYGGAEDSYTKAVKVCPACYQKDRSVLFSNRAAARMKQDKTEAALSDCSKAIELDPNYIRALLRRAELYEKTEKLDEALEDYKTVLEKDPSVHHAREACMRLPRQIEERNERLKTEMLGKLKDLGNLVLRPFGLSTENFQVKQDSSTGSYSINFVQNPNNNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFT4</accession>
    <name>A0A452GFT4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GFT4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000365"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000410.1">
        <property type="protein sequence ID" value="ENSGAGP00000000365.1"/>
        <property type="gene ID" value="ENSGAGG00000000300.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003655">
        <property type="entry name" value="aKRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF133">
        <property type="entry name" value="RIKEN CDNA 1700020N01 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50806">
        <property type="entry name" value="KRAB_RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB-related" evidence="2">
        <location>
            <begin position="2"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="5"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="122" mass="14079" checksum="1CA21BB36668DFB6" modified="2019-05-08" version="1">LQLQVAFEDVALYFTREEWELLSQREKHLYRDQMLRNYWALVSLGKDQLRFFLTAVKCLGSLHTSASVIIIKSLLIYTPASEGLLDVEGKGRARSAGWEQKDSLHLEVFKPQFEDFNNSDIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGC8</accession>
    <name>A0A452GGC8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutaminase</fullName>
            <ecNumber evidence="3">3.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452GGC8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000629"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000706.1">
        <property type="protein sequence ID" value="ENSGAGP00000000629.1"/>
        <property type="gene ID" value="ENSGAGG00000000530.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.210:FF:000001">
        <property type="entry name" value="Glutaminase kidney isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.710.10:FF:000002">
        <property type="entry name" value="glutaminase kidney isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000019">
        <property type="entry name" value="Glutaminase liver isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00313">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015868">
        <property type="entry name" value="Glutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041541">
        <property type="entry name" value="Glutaminase_EF-hand"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03814">
        <property type="entry name" value="Gln_ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544:SF49">
        <property type="entry name" value="GLUTAMINASE KIDNEY ISOFORM, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17959">
        <property type="entry name" value="EF-hand_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04960">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glutaminase EF-hand" evidence="13">
        <location>
            <begin position="10"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="517"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="523"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="539" mass="60288" checksum="FCDAD8D01A8B8AF9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIQ6</accession>
    <name>A0A452GIQ6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GIQ6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001547"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001763.1">
        <property type="protein sequence ID" value="ENSGAGP00000001547.1"/>
        <property type="gene ID" value="ENSGAGG00000001250.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="225" mass="24764" checksum="228EE6C0AE447C2E" modified="2019-05-08" version="1">MPWLPGAVWCGPQPCAPAGAGPSRVVRPLTLHPSWSGAEASTSSGEQRESRKNNQDEIDKLLLDLEHFSQKMEGALKEPTMKENNPGFLKGFGQEGGNVLPLALETKNKVSLFPDTNSTSPLSKTVETNGHKMEEDDKALLLRILESIEDFAQELVECHMGKGSLSKEKEMMQILQETLTTPSQSFPAVQRSCMGATTKDTVPTLIQQAPEVIKVRHQSLWLDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJ97</accession>
    <name>A0A452GJ97_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GJ97"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001666"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001892.1">
        <property type="protein sequence ID" value="ENSGAGP00000001666.1"/>
        <property type="gene ID" value="ENSGAGG00000001331.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051171">
        <property type="entry name" value="CaCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004836">
        <property type="entry name" value="Na_Ca_Ex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878">
        <property type="entry name" value="SODIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878:SF6">
        <property type="entry name" value="SODIUM_CALCIUM EXCHANGER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01259">
        <property type="entry name" value="NACAEXCHNGR"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="10">
        <location>
            <begin position="100"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="185" mass="20664" checksum="39E7E609976E29FB" modified="2019-05-08" version="1">MGRPVLGEHTKLEVIIEESYEFKNTVDKLIKKTNLALVVGTNSWREQFIEAITVSAGEEDDDDECGEEKLPSCFDYVMHFLTVFWKVLFAFVPPTDYWNGWACFVVSILMIGLLTAFIGDLASHFGCTIGLKDSVTAVVFVALGTSVPGKKNRLLVVPWSAKVMCQSTFLLSPLPAECWGFLHFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJN4</accession>
    <name>A0A452GJN4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">L-Fucosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a ganglioside GM1 + GDP-beta-L-fucose = a ganglioside Fuc-GM1 + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:82639"/>
            <dbReference type="ChEBI" id="CHEBI:90189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:48293"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Golgi apparatus</location>
            <location evidence="1 7">Golgi stack membrane</location>
            <topology evidence="1 7">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glycosyltransferase 11 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452GJN4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001902"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002161.1">
        <property type="protein sequence ID" value="ENSGAGP00000001902.1"/>
        <property type="gene ID" value="ENSGAGG00000001524.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008107">
        <property type="term" value="F:galactoside 2-alpha-L-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11301">
        <property type="entry name" value="Fut1_Fut2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002516">
        <property type="entry name" value="Glyco_trans_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11927">
        <property type="entry name" value="GALACTOSIDE 2-L-FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11927:SF9">
        <property type="entry name" value="L-FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01531">
        <property type="entry name" value="Glyco_transf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0812">Transmembrane</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="334" mass="38460" checksum="F43E33448C7494B5" modified="2019-05-08" version="1">HPFSCSSFYLPIHPFPIFLSTLPSIPTLPSILPSIPILPSIHLSIYPSPQGMWTVNSAGRLGNQMGEYATLYALAKMNRRQAYILPEMHQQLAPLFRITLPVLSRFMASRIPWKDYELHDWMTEEYRHIEGKYIWLTGYPCSWTFYHHLRQEILQEFSFHDHVKEEANRYLAGLRGQRWNVTYVGVHVRRGDYVRVMTKKWKGVVADKAYLEKAMGYFRAKYQEPVFVVTSNGMEWCRENIDTSRGDVYFSGDGKESSPGRDFALLAHCNHTIMTIGTFGIWASYLAGGETVYLANYTLPDSHFLEIFKPWATFLPEWIGIDADLSPLRSGTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKQ4</accession>
    <name>A0A452GKQ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GKQ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002326"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002667.1">
        <property type="protein sequence ID" value="ENSGAGP00000002326.1"/>
        <property type="gene ID" value="ENSGAGG00000001899.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035259">
        <property type="term" value="F:nuclear glucocorticoid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071376">
        <property type="term" value="P:cellular response to corticotropin-releasing hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048384">
        <property type="term" value="P:retinoic acid receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06964">
        <property type="entry name" value="NR_DBD_RAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06937">
        <property type="entry name" value="NR_LBD_RAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000001">
        <property type="entry name" value="Retinoic acid receptor beta isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000004">
        <property type="entry name" value="Retinoic acid receptor beta isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047159">
        <property type="entry name" value="NR_DBD_RAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047158">
        <property type="entry name" value="NR_LBD_RAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003078">
        <property type="entry name" value="Retinoic_acid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24085">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24085:SF7">
        <property type="entry name" value="RETINOIC ACID RECEPTOR GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01292">
        <property type="entry name" value="RETNOICACIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="14">
        <location>
            <begin position="35"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="15">
        <location>
            <begin position="133"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="353"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="400" mass="45198" checksum="A9F270BC0C94288F" modified="2019-05-08" version="1">MPLPFPLSSAVETQSTSSEEMVPSSPSPPPPPRVYKPCFVCNDKSSGYHYGVSSCEGCKGFFRRSIQKNMVYTCHRDKNCQINKVTRNRCQYCRLQKCFEVGMSKEAVRNDRNKKKKDVKEEVVVDSYEMTPELEELIQKVSKAHQETFPSLCQLGKYTTNSSADHRVQLDLGLWDKFSELATKCIIKIVEFAKRLPGFTTLSIADQITLLKAACLDILMLRICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFAGQLLPLEMDDTETGLLSAICLICGDRMDLEEPEKVDKLQEPLLEALKIYARRRRPNKPYMFPRMLMKITDLRGISTKGAERAITLKMEIPGPMPPLIREMLENPEMFEDDSSQPPPSAEPPSSEESPAESKSPEETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLK5</accession>
    <name>A0A452GLK5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger protein 850</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000002684"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003076.1">
        <property type="protein sequence ID" value="ENSGAGP00000002684.1"/>
        <property type="gene ID" value="ENSGAGG00000002154.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000056">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000355">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 20 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000252">
        <property type="entry name" value="Zinc finger protein 287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000367">
        <property type="entry name" value="Zinc finger protein 572"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001437">
        <property type="entry name" value="Zinc finger protein 594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000466">
        <property type="entry name" value="zinc finger protein 62 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001745">
        <property type="entry name" value="Zinc finger protein 658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000953">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001157">
        <property type="entry name" value="Zinc finger protein 793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF73">
        <property type="entry name" value="ZINC FINGER PROTEIN 572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="26"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="13">
        <location>
            <begin position="8"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="236"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="264"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="292"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="487"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="515"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="543"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="571"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="599"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="647"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="675"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="703"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="731"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="759"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="787"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="815"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="873" mass="100208" checksum="0F1EF5ED58C7AF3B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLM7</accession>
    <name>A0A452GLM7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Alpha-tectorin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">One of the major non-collagenous components of the tectorial membrane. The tectorial membrane is an extracellular matrix of the inner ear that covers the neuroepithelium of the cochlea and contacts the stereocilia bundles of specialized sensory hair cells. Sound induces movement of these hair cells relative to the tectorial membrane, deflects the stereocilia and leads to fluctuations in hair-cell membrane potential, transducing sound into electrical signals.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">May form homomeric filament after self-association or heteromeric filament after association with beta-tectorin. Interacts with CEACAM16.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
            <orientation evidence="1">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000002695"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003089.1">
        <property type="protein sequence ID" value="ENSGAGP00000002695.1"/>
        <property type="gene ID" value="ENSGAGG00000002143.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000055">
        <property type="entry name" value="alpha-tectorin isoform X1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000451">
        <property type="entry name" value="alpha-tectorin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000001">
        <property type="entry name" value="alpha-tectorin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3210:FF:000002">
        <property type="entry name" value="Tectorin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052749">
        <property type="entry name" value="Alpha-tectorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003886">
        <property type="entry name" value="NIDO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017977">
        <property type="entry name" value="ZP_dom_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160:SF3">
        <property type="entry name" value="ALPHA-TECTORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160">
        <property type="entry name" value="ALPHA-TECTORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06119">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00539">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51220">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00682">
        <property type="entry name" value="ZP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9" id="KW-1009">Hearing</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019030245" description="Alpha-tectorin" evidence="17">
        <location>
            <begin position="23"/>
            <end position="2099"/>
        </location>
    </feature>
    <feature type="domain" description="NIDO" evidence="19">
        <location>
            <begin position="98"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="20">
        <location>
            <begin position="320"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="20">
        <location>
            <begin position="711"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="20">
        <location>
            <begin position="1098"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="18">
        <location>
            <begin position="1749"/>
            <end position="2003"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2099" mass="233607" checksum="AD15A10EDAFE25AA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GM14</accession>
    <name>A0A452GM14_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="8">PARP</shortName>
            <ecNumber evidence="8">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A452GM14"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002825"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003229.1">
        <property type="protein sequence ID" value="ENSGAGP00000002825.1"/>
        <property type="gene ID" value="ENSGAGG00000002253.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01439">
        <property type="entry name" value="TCCD_inducible_PARP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000003">
        <property type="entry name" value="TCDD-inducible poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051712">
        <property type="entry name" value="ARTD-AVP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740:SF4">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02825">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="2 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="WWE" evidence="9">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="10">
        <location>
            <begin position="124"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="338" mass="39451" checksum="87856A9BD169CE12" modified="2019-05-08" version="1">MWGAADAEMLQRASEEFSPKMEIPEEEMDTTDTQWGWFYLAECGKWHMFQTDSNTQCSVSSEDIERSFKMNPEGSVSFTTAKFNYKLDFSVMKQTNLTTGKQRPIKRAPFSISAFSYICENEAIPMPSHWENVNTEEPYQLIPLQKQTNEYNEVSSLFGKTMDSNRIKRIQRIQNLDLWEFFCRKKSQLKKRRGVPIINEQMLFHGTSNEFVEAICIHNFDWRINGMHAAVYGKGTYFARDASYSSRFCKDDMKHGDTFQIHGVSLQHHVLRTYKVMFLARVLTGDYVIGDSKYMRPPSKDGSFVNLFDSCVDSTWNPKIFVIFDTNQIYPEYLIEFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GN99</accession>
    <name>A0A452GN99_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="2">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:33180"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452GN99"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003200"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003689.1">
        <property type="protein sequence ID" value="ENSGAGP00000003200.1"/>
        <property type="gene ID" value="ENSGAGG00000002583.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060470">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration involved in egg activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000147">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF29">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE ZETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="123" mass="14105" checksum="E2B7BDE41843F04E" modified="2019-05-08" version="1">MQLISGYQLPPSNLSRTNKADPLVQIEIHGVPEDQVKQQTCVIKSNALCPRWNETFTFNIQVPELALVRFSVEDQISLAANEFLGQYTLPLLCMNKGYRHVPLFSKLGDRLDPASLFVYIWYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNR6</accession>
    <name>A0A452GNR6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000003377"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003892.1">
        <property type="protein sequence ID" value="ENSGAGP00000003377.1"/>
        <property type="gene ID" value="ENSGAGG00000002730.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="201" mass="21717" checksum="EC089991932D8546" modified="2019-05-08" version="1">MGQGVSLAAGGGRPGLSRDRLQAPPTTSPSRPSAAGTHPAAHPQAVRPGGDTPGLSCLHACGGGRWGGPCVRPPDPSVRPAGGPHHPFLVVYDLKPFKSQGPVFAKGVPVTARVEGTERYTTGSKIRPSTLYSLRLTHGDFTWTMKKKFRHFQELHRDLMRHKIFMSFLPLSRSEPPPPXLCRRHPPQPRSLLPHTAAAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GP19</accession>
    <name>A0A452GP19_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GP19"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003652"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004217.1">
        <property type="protein sequence ID" value="ENSGAGP00000003652.1"/>
        <property type="gene ID" value="ENSGAGG00000002958.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039938">
        <property type="entry name" value="Sp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14947">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14947:SF24">
        <property type="entry name" value="ZINC FINGER PROTEIN 781-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="131" mass="14235" checksum="CDF2F822C23C30E7" modified="2019-05-08" version="1">MNAVSALLLIRGITQERNPMNAVSAGKPSLGAHTLLDIRGATQERNPINAVSVEKPLLRAHTLLYIRGATLERNLMNAVSVEKPLQHLWCLCSFTEAHSVFHQLLCSASWKCASLQECPSAPGGGTRSDLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQI2</accession>
    <name>A0A452GQI2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQI2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004203"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004928.1">
        <property type="protein sequence ID" value="ENSGAGP00000004203.1"/>
        <property type="gene ID" value="ENSGAGG00000003484.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008508">
        <property type="term" value="F:bile acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000045">
        <property type="entry name" value="Solute carrier family 10 member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004710">
        <property type="entry name" value="Bilac:Na_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361">
        <property type="entry name" value="SODIUM-BILE ACID COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361:SF41">
        <property type="entry name" value="SODIUM_BILE ACID COTRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="284" mass="31225" checksum="188A45CE15837739" modified="2019-05-08" version="1">MDFYRQLLLEAREVLFPPLPPAADGRAARADPLPVVLSPPSCSIIMTASSTLLALLLMPLCLWIYSRAWINTPLVQLLPLGAVSLTLCSTLLPIGVGVLIRYKYTRVADLLLKVSLWSLLVTLVLLFILTGTMLGPELLASIPASVYIVALLMPLAGYASGYGLATLFHLPPHCKRTVSLETGCQNVQLCTAILKLTFPPHLIGSMYMFPLLYALFQAAEAGIFVLVYKMYGRDAYKQDPLNEDEDTDISYKKLKEEEMADTSYGTVTTEEHNSILMEPAQTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQK3</accession>
    <name>A0A452GQK3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rad60/SUMO-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GQK3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004212"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004939.1">
        <property type="protein sequence ID" value="ENSGAGP00000004212.1"/>
        <property type="gene ID" value="ENSGAGG00000003493.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000195">
        <property type="entry name" value="small ubiquitin-related modifier 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad60/SUMO-like" evidence="2">
        <location>
            <begin position="16"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="56" mass="6467" checksum="E266A8D89C62CBE0" modified="2019-05-08" version="1">FSPQQEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLNKLMKAYCERQVRCLCEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQY1</accession>
    <name>A0A452GQY1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G2/mitotic-specific cyclin-B1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for the control of the cell cycle at the G2/M (mitosis) transition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family. Cyclin AB subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GQY1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004338"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005098.1">
        <property type="protein sequence ID" value="ENSGAGP00000004338.1"/>
        <property type="gene ID" value="ENSGAGG00000003605.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20565">
        <property type="entry name" value="CYCLIN_CCNB1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20569">
        <property type="entry name" value="CYCLIN_CCNB1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000027">
        <property type="entry name" value="G2/mitotic-specific cyclin-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048026">
        <property type="entry name" value="CCNB1_first_cyclin-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5 8" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="10">
        <location>
            <begin position="183"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="11">
        <location>
            <begin position="276"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="10">
        <location>
            <begin position="280"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="22"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="410" mass="45627" checksum="2C7F6FF6B4FB0954" modified="2019-05-08" version="1">MALRISRNTRVNAENQVKSNVAAAKRGAAAAKPGLRPRTALGDIGNKVGEQPPKALPKKETKPPSAAVKPLPRKATRAAEKVIEPAKEQKPIPEPVKLESPSPSPMETSGCVPGEEMLCQAFSDVLLEVKDVDVDDGADPNLCSEYVKDIYGYLRDLEKQQNVRPKYLAGQEVTGNMRAILIDWLVQVQMKFRLLSETMYMTAAIIDRFLQDNSVPKKMLQLVGVTAMFIASKYEEMYPPEIGDFAFVTDHTYTKSQIKQMERKILQALDFTLGRPLPLHFLRRASKIGEVDLEQHTLAKYLMELSLVDYEMVHYPPSQIAAAAFCLALKVLDGGEWTPTLQHYMSYTESALHPVMQHMAKNVILVNRGLTKHMTVKNKYASSKNAKISTLPQLNSTVIQDLAKPVSKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GS12</accession>
    <name>A0A452GS12_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GS12"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004496"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005270.1">
        <property type="protein sequence ID" value="ENSGAGP00000004496.1"/>
        <property type="gene ID" value="ENSGAGG00000003715.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000054">
        <property type="entry name" value="Adhesion G protein-coupled receptor E3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001740">
        <property type="entry name" value="GPCR_2_EMR1-like_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF433">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR E1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01128">
        <property type="entry name" value="EMR1HORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="211"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="14">
        <location>
            <begin position="62"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="349" mass="39152" checksum="2F68D04C48BFB2E7" modified="2019-05-08" version="1">MRMRIILWCFQAKKEEEEAFCIYWKVVAKSGSWSPDGCTTVHTNSTHTTLTVSHRETWSYPLTIITYVGLTLSLLCLFLAILTFLLCRSICNVSTSLHLQLCLCLFLADLLFLTAATRTGSRVACAVIAGLLHYLFLACFSWMFLEGLHLFLTVWNLKVVNYTSASRFKKRFMYPVGYGFPALVVAISAGVNPEGYRTSTHCWLSLERGFHWSFLGPVCAIILINIMFFALTLWILRNRLSSLNADVSTLRDHRLLTFKAIAQLFILGCTWSVGLLQVGPAATVMAYLFTIINSLQGAFIFLVHCLLNCQVTPTAHQCSSSTFTGLAVAISQSRNTPTKKPSEKLQNWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GS30</accession>
    <name>A0A452GS30_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GS30"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004514"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005290.1">
        <property type="protein sequence ID" value="ENSGAGP00000004514.1"/>
        <property type="gene ID" value="ENSGAGG00000003734.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000005296.1">
        <property type="protein sequence ID" value="ENSGAGP00000004519.1"/>
        <property type="gene ID" value="ENSGAGG00000003734.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032809">
        <property type="term" value="C:neuronal cell body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005330">
        <property type="term" value="F:dopamine:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005328">
        <property type="term" value="F:neurotransmitter:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005334">
        <property type="term" value="F:norepinephrine:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051583">
        <property type="term" value="P:dopamine uptake involved in synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11512">
        <property type="entry name" value="SLC6sbd_NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002435">
        <property type="entry name" value="Na/ntran_symport_noradrenaline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF320">
        <property type="entry name" value="SODIUM-DEPENDENT NORADRENALINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01201">
        <property type="entry name" value="NORTRANSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0915">Sodium</keyword>
    <keyword evidence="7 15" id="KW-0769">Symport</keyword>
    <keyword evidence="4 15 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="286"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="332"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="368"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="413"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="468"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="499"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="543"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="641" mass="71676" checksum="D22F9F219E4FCBC3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GS70</accession>
    <name>A0A452GS70_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the S-100 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GS70"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004809"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005628.1">
        <property type="protein sequence ID" value="ENSGAGP00000004809.1"/>
        <property type="gene ID" value="ENSGAGG00000003951.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043542">
        <property type="term" value="P:endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00213">
        <property type="entry name" value="S-100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034325">
        <property type="entry name" value="S-100_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013787">
        <property type="entry name" value="S100_Ca-bd_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639:SF134">
        <property type="entry name" value="PROTEIN S100-A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639">
        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01023">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01394">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="47"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="119" mass="13698" checksum="B292D4B21F65AA34" modified="2019-05-08" version="1">MTSKLEKALDDMIDVYHKYSEKRPNSDDYLQKHEFRQLLKQNAKSFLKYEYIDDLFKKADRNRNEYLHFEEFVTTLAKLAIHAHNISHEPCDETPGKPGDGQGQGQGHGHGHGHGHGPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSM2</accession>
    <name>A0A452GSM2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GSM2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004989"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005827.1">
        <property type="protein sequence ID" value="ENSGAGP00000004989.1"/>
        <property type="gene ID" value="ENSGAGG00000004074.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070915">
        <property type="term" value="F:lysophosphatidic acid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000025">
        <property type="entry name" value="Lysophosphatidic acid receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004065">
        <property type="entry name" value="LPA_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002277">
        <property type="entry name" value="LPA_rcpt_EDG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01148">
        <property type="entry name" value="EDG2RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01527">
        <property type="entry name" value="LPARECEPTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="45"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="337" mass="38655" checksum="47AA2EA63F70346A" modified="2019-05-08" version="1">MKCYYNENIAFFYNRSGKYLATEWNTVSKLVMGLGITVCIFIMLANLLVMVAIYVNRRFHFPIYYLMANLAAADFFAGLAYFYLMFNTGPNTRRLTVSTWLLRQGLIDTSLTASVANLLAIAIERHITVFRMQLHTRMSNRRVVVVIVVIWTMAIVMGAIPSVGWNCICDITHCSNMAPLYSDSYLVFWAVFNLVTFLVMVVLYAHIFGYVRQRTMRMSRHSSGPRRNRDTMMSLLKTVVIVLGEYLLQQKCFSLFPVARQSFRTLFPWIALADSIARRPWSPFSFFFFTVTVCVLDAADRGVTAALHSSIHLLLHDSRDGYQSFCTVCCHCKLAMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSR3</accession>
    <name>A0A452GSR3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GSR3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004763"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005577.1">
        <property type="protein sequence ID" value="ENSGAGP00000004763.1"/>
        <property type="gene ID" value="ENSGAGG00000003910.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042269">
        <property type="term" value="P:regulation of natural killer cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051527">
        <property type="entry name" value="KLR_subfamily_B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46784">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR SUBFAMILY B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46784:SF1">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR SUBFAMILY B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11840" checksum="76C1E80A9986FAE0" modified="2019-05-08" version="1">MLPHHLPLRCTRRPPMGKATFLLDGSHFLFCMIFGWTGEITSLSSFPTPEGSSCRLCPTEWLMHEGKCYWASKESQIWHKSHDDCSAKSSRLLVIQDQKEMVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTN9</accession>
    <name>A0A452GTN9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GTN9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005082"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005925.1">
        <property type="protein sequence ID" value="ENSGAGP00000005082.1"/>
        <property type="gene ID" value="ENSGAGG00000004136.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000004">
        <property type="entry name" value="cytochrome P450 2D15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF411">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 2, SUBFAMILY AB, POLYPEPTIDE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="489" mass="56150" checksum="708B463477F90398" modified="2019-05-08" version="1">AMLISEFLVALLISLFVVQFLKLKWACSRLPPGPTPLPLIGTVWQMDFKRQHELLIKLAQIYGNIFTLWVGHVPVIILNGFTAVKDGLTVHSEDVSGRPATPFLQKTARGKGIIFTSGHTWKQQRRFGLMTLRNLGLGKKGLEHRIQEEARQLLEYFMNERGEPLDPSFPIVHSVSNVIAAVVFGHRFSIEDKYFHQLIENNDYLHISRLPFSQLYDVFPWLMDHLPGSHQKIFYCQEFLRSFARKEVRSHQERGSPDEPQDFIDFYLAQIDKSKADPHSTFDEDNLVQTIFELFLAGTETTTTTMRWAVLNMVAYPDIQERVQKELDTVLGPSRLICYEDRKNLPYTNAVIHEIQRRSSIIAVGVPRECVKDTMLQGFPVEKGTIILPNLTSALLDPEQWETPRQFNPNHFLDQDGNFVKKEAFLPFSAGHRVCLGEQLARTELFIFFTNLLRAFTFRLPEGVKEVNTEFVLGGTLQPHPYKICAIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUK9</accession>
    <name>A0A452GUK9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GUK9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005449"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006344.1">
        <property type="protein sequence ID" value="ENSGAGP00000005449.1"/>
        <property type="gene ID" value="ENSGAGG00000004423.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043256">
        <property type="term" value="C:laminin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070831">
        <property type="term" value="P:basement membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000084">
        <property type="entry name" value="Laminin subunit alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000101">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000440">
        <property type="entry name" value="Laminin subunit beta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000073">
        <property type="entry name" value="Laminin subunit beta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000135">
        <property type="entry name" value="Laminin subunit beta 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000001">
        <property type="entry name" value="Laminin subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000333">
        <property type="entry name" value="netrin-4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056558">
        <property type="entry name" value="LAMB1-4_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF268">
        <property type="entry name" value="LAMININ SUBUNIT BETA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23219">
        <property type="entry name" value="LAMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 12" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Laminin N-terminal" evidence="16">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="187"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="253"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="316"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="368"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="418"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="471"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="984"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="765"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="889"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="418"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="471"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="473"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="492"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="504"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1104" mass="122559" checksum="A94BF3EBD4F5975B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GUL4</accession>
    <name>A0A452GUL4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 13">Histone deacetylase</fullName>
            <ecNumber evidence="2 13">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-(2E)-butenoyl-L-lysyl-[protein] + H2O = (2E)-2-butenoate + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:69172"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13707"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:35899"/>
            <dbReference type="ChEBI" id="CHEBI:137954"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:69173"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:58197"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-acetyl-L-lysyl-[protein] + H2O = L-lysyl-[protein] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58108"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:58109"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2 13"/>
    <dbReference type="AlphaFoldDB" id="A0A452GUL4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005786"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006748.1">
        <property type="protein sequence ID" value="ENSGAGP00000005786.1"/>
        <property type="gene ID" value="ENSGAGG00000004705.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160008">
        <property type="term" value="F:protein decrotonylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10011">
        <property type="entry name" value="HDAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000003">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF3">
        <property type="entry name" value="HISTONE DEACETYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 13" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="18">
        <location>
            <begin position="29"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="389"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="402"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="418"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="429"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="488" mass="55292" checksum="67550C16EC5E07FA" modified="2019-05-08" version="1">MAYSQGGGKKKVCYYYDGDIGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEIYRPHKATAEEMTKYHSDEYIKFLRSIRPDNMSEYSKQMQRFNVGEDCPVFDGLFEFCQLSTGGSVAGAVKLNRQQTDMAVNWAGGLHHAKKSEASGFCYVNDIVLAILELLKYHQRVLYIDIDIHHGDGVEEAFYTTDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNFPMRDGIDDESYGQIFKPIISKVMEMYQPSAVVLQCGADSLSGDRLGCFNLTVKGHAKCVEVVKTFNLPLLMLGGGGYTIRNVARCWTYETAVALDCEIPNELPYNDYFEYFGPDFKLHISPSNMTNQNTPEYMEKIKQRLFENLRMLPHAPGVQMQAIPEDAVHEDSGDEDGEDPDKRISIRASDKRIACDEEFSDSEDEGEGGRRNVADHKKGAKKARIEEDKKETEDKKTDVKEEDKSKDSSGEKTDTKGAKSELLSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GY29</accession>
    <name>A0A452GY29_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
            <ecNumber evidence="9">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Dual specificity phosphatase able to dephosphorylate phosphotyrosine, phosphoserine and phosphothreonine residues, with a preference for phosphotyrosine as a substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EC" id="3.1.3.48" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452GY29"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007078"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008196.1">
        <property type="protein sequence ID" value="ENSGAGP00000007078.1"/>
        <property type="gene ID" value="ENSGAGG00000005687.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033549">
        <property type="term" value="F:MAP kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14580">
        <property type="entry name" value="DUSP13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000037">
        <property type="entry name" value="dual specificity protein phosphatase 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020405">
        <property type="entry name" value="Atypical_DUSP_subfamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682">
        <property type="entry name" value="AGAP008228-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682:SF3">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01909">
        <property type="entry name" value="ADSPHPHTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="10">
        <location>
            <begin position="42"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="11">
        <location>
            <begin position="111"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="8">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR620405-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="194" mass="21576" checksum="E03769AD12D34C72" modified="2019-05-08" version="1">MTMAEGDVLLAKDPEGSETCTAEVPTVKDIEQLLNTGLSSCNHTDEVWPNLFLGDLVTAHNRFGLWKMGITHVLNAAHGTMFCQGGQDFYGTTIDYYGIPAYDLPDFDISQYFFSAAEFIHKALNTPGAKILVHCAVGISRSASLVLAYLMINHHLPLIKAIKAVKEHRWISPNRGFLKQLRNLDIQLRQTRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZH1</accession>
    <name>A0A452GZH1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytokine receptor common subunit beta N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GZH1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007585"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008756.1">
        <property type="protein sequence ID" value="ENSGAGP00000007585.1"/>
        <property type="gene ID" value="ENSGAGG00000006070.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048668">
        <property type="entry name" value="IL3RB_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21460">
        <property type="entry name" value="IL3Rb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytokine receptor common subunit beta N-terminal" evidence="1">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="65" mass="7395" checksum="35C7746C288C9F96" modified="2019-05-08" version="1">HSPWVKRCTGLCYRCAFLPPAESLPMQSLHCYNDFTSQMTCTWQECTAARCFLSVTLHHENTGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H2C6</accession>
    <name>A0A452H2C6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Heat shock protein family A (Hsp70) member 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H2C6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008658"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009952.1">
        <property type="protein sequence ID" value="ENSGAGP00000008658.1"/>
        <property type="gene ID" value="ENSGAGG00000006849.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000012">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="607"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="638" mass="70110" checksum="AC31B74D27EB34A4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3G5</accession>
    <name>A0A452H3G5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ubiquitin carboxyl-terminal hydrolase 32</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Deubiquitinating enzyme 32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Ubiquitin thioesterase 32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Ubiquitin-specific-processing protease 32</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Deubiquitinase that can remove conjugated ubiquitin from target proteins, such as RAB7A and LAMTOR1. Acts as a positive regulator of the mTORC1 signaling by mediating deubiquitination of LAMTOR1, thereby promoting the association between LAMTOR1 and the lysosomal V-ATPase complex and subsequent activation of the mTORC1 complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Golgi apparatus membrane</location>
            <topology evidence="16">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008962"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010295.1">
        <property type="protein sequence ID" value="ENSGAGP00000008962.1"/>
        <property type="gene ID" value="ENSGAGG00000007083.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000081">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000128">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000068">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2230.10:FF:000004">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000018">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000258">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000110">
        <property type="entry name" value="ubiquitin carboxyl-terminal hydrolase 32 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028135">
        <property type="entry name" value="Ub_USP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057368">
        <property type="entry name" value="USP32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF76">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14836">
        <property type="entry name" value="Ubiquitin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25265">
        <property type="entry name" value="USP32_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0636">Prenylation</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="EF-hand" evidence="23">
        <location>
            <begin position="228"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="23">
        <location>
            <begin position="264"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="25">
        <location>
            <begin position="369"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="24">
        <location>
            <begin position="734"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1323"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1361"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="1388"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1426"/>
            <end position="1442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00071642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00078963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00082194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1607" mass="182483" checksum="21CAD7401A1C0055" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3W7</accession>
    <name>A0A452H3W7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Stonin-2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H3W7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009145"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010504.1">
        <property type="protein sequence ID" value="ENSGAGP00000009145.1"/>
        <property type="gene ID" value="ENSGAGG00000007227.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022699">
        <property type="entry name" value="Stonin2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12016">
        <property type="entry name" value="Stonin2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Stonin-2 N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="128" mass="13757" checksum="975A014C9FD2BBAE" modified="2019-05-08" version="1">MTSLANVIASHQSEWVSFNDELLVPVISQGGSEEPLEKCFSSSDTSSEDNHNVDGEFQDLSHTELSDDVDKPGVDSASVSLLGNHVCPKPDLSSSISNWVQFEDVPWTSTLPAHIQTGRGASCKGLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3X7</accession>
    <name>A0A452H3X7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Caspase-9</fullName>
            <ecNumber evidence="11">3.4.22.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Apoptotic protease Mch-6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Apoptotic protease-activating factor 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">ICE-like apoptotic protease 6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-|-Xaa.</text>
            <dbReference type="EC" id="3.4.22.62"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 17">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.62" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A452H3X7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009292"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010672.1">
        <property type="protein sequence ID" value="ENSGAGP00000009292.1"/>
        <property type="gene ID" value="ENSGAGG00000007345.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009411">
        <property type="term" value="P:response to UV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08326">
        <property type="entry name" value="CARD_CASP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00032">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000041">
        <property type="entry name" value="Caspase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000012">
        <property type="entry name" value="Caspase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042147">
        <property type="entry name" value="CARD_CASP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033139">
        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016129">
        <property type="entry name" value="Caspase_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901:SF8">
        <property type="entry name" value="CASPASE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038001">
        <property type="entry name" value="Caspase_ICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00114">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="CARD" evidence="21">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="20">
        <location>
            <begin position="166"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="19">
        <location>
            <begin position="338"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="301"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR038001-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="423" mass="47151" checksum="46B2CBDB8E91DA70" modified="2019-05-08" version="1">MEEAQRALLQRSRLRLVGELQVAPLWDLLLHKGVFTQDMIEEIQRAGTRRDQARQLVTDLQTRGRQAFPAFVSSLRETGQNDLVALLSEGDRFPQLRPLDLKPVEMGLRGGKGDTYGKAREYLPTPVQAVTKRCQESTHPSAAARGSAAETSRRNSDMAYVLKADPCGYCLIINNVRFIEESDLSSREGSNVDCEKLEKRFKSLHFDVLVRRDLKAQEIAGELQRLARQDHSALDCCLVVILSHGCQTSHIQFPGGIYGTDGKHIPVEKIVNFFNGSHCPSLRGKPKLFFIQACGGEQKDRGFEVDSDSPVNQPRGGSVESDATPFQTQPGNLDEPDAVASLPTPSDILVSYSTFPGFVSWRDTQSGSWYIETLDSVFEQYACTEDLLGMLVRVAHAVSAKGKYKQMPGTFNFLRKQFFFVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4B6</accession>
    <name>A0A452H4B6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Lipid transferase CIDEA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Cell death activator CIDE-A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Cell death-inducing DFFA-like effector A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a triacyl-sn-glycerol(in) = a triacyl-sn-glycerol(out)</text>
            <dbReference type="Rhea" id="RHEA:39011"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Interacts with CIDEC. Directly interacts with CEBPB. Interacts with isoform CLSTN3beta of CLSTN3; inhibiting the lipid transferase activity of CIDEA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CIDE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H4B6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009444"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010851.1">
        <property type="protein sequence ID" value="ENSGAGP00000009444.1"/>
        <property type="gene ID" value="ENSGAGG00000007476.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000010855.1">
        <property type="protein sequence ID" value="ENSGAGP00000009448.1"/>
        <property type="gene ID" value="ENSGAGG00000007476.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160077">
        <property type="term" value="P:lipid droplet fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050995">
        <property type="term" value="P:negative regulation of lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06539">
        <property type="entry name" value="CIDE_N_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.10:FF:000008">
        <property type="entry name" value="Cell death inducing DFFA like effector a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003508">
        <property type="entry name" value="CIDE-N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032936">
        <property type="entry name" value="CIDEA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12306">
        <property type="entry name" value="CELL DEATH ACTIVATOR CIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12306:SF8">
        <property type="entry name" value="LIPID TRANSFERASE CIDEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02017">
        <property type="entry name" value="CIDE-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00266">
        <property type="entry name" value="CAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51135">
        <property type="entry name" value="CIDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4 15" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CIDE-N" evidence="16">
        <location>
            <begin position="37"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="217" mass="24106" checksum="BA4BDACFCE7F3B42" modified="2019-05-08" version="1">MESARDYAGALVRSLVSMGTSVGSVTRQTLFPPLTPAARPFRVSNHDRSSRKGVVASSLQELISKALDALLITAGLITIVLEEDGTVVDTEEFFQSLGDNTHFILLEKGQKWTQGENYILAVQQTKKMGVANITLDLYKLNPKDFIGCLNIRATFYEIYSVSYDIKCMGAKTVLRRMLRFVSHVAQITGQFLLYTGSYVLQLMGECEEDGMCTNSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4E5</accession>
    <name>A0A452H4E5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein jagunal homolog 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the jagunal family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H4E5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009481"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010886.1">
        <property type="protein sequence ID" value="ENSGAGP00000009478.1"/>
        <property type="gene ID" value="ENSGAGG00000007497.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000010890.1">
        <property type="protein sequence ID" value="ENSGAGP00000009481.1"/>
        <property type="gene ID" value="ENSGAGG00000007497.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038158">
        <property type="term" value="P:granulocyte colony-stimulating factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009787">
        <property type="entry name" value="Jagunal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20955">
        <property type="entry name" value="PROTEIN JAGUNAL HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20955:SF1">
        <property type="entry name" value="PROTEIN JAGUNAL HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07086">
        <property type="entry name" value="Jagunal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="183" mass="21119" checksum="5C3E288B9872970A" modified="2019-05-08" version="1">MASRVGPRAEGTDGSDYQHRERVASHYQMSVALKSEIKKLIYMQMAIWLLLLAQMCVGHLKLLPHDQVAMPYQWEYPYLLSIIPSLFGLMSFPRNNISYLVLSMISTGLFSVAPLIYGSMEMFPMAQQLYRHGKAYRFIFGFSAVSVMYLLVVVAVQVHGWQLYYSKKLLDSWFTSTQEKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6C6</accession>
    <name>A0A452H6C6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Speckle targeted PIP5K1A-regulated poly(A) polymerase</fullName>
            <ecNumber evidence="6">2.7.7.19</ecNumber>
            <ecNumber evidence="7">2.7.7.52</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">RNA-binding motif protein 21</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">U6 snRNA-specific terminal uridylyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>RNA(n) + UTP = RNA(n)-3'-uridine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14785"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17348"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173116"/>
            <dbReference type="EC" id="2.7.7.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="6"/>
    <dbReference type="EC" id="2.7.7.52" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452H6C6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010085"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011574.1">
        <property type="protein sequence ID" value="ENSGAGP00000010085.1"/>
        <property type="gene ID" value="ENSGAGG00000007912.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050265">
        <property type="term" value="F:RNA uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12279">
        <property type="entry name" value="RRM_TUT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000305">
        <property type="entry name" value="speckle targeted PIP5K1A-regulated poly(A) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034388">
        <property type="entry name" value="Star-PAP_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF127">
        <property type="entry name" value="SPECKLE TARGETED PIP5K1A-REGULATED POLY(A) POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18 25" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="27">
        <location>
            <begin position="53"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="592"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00021679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00033036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="757" mass="84493" checksum="F3EF757E4CB5784F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7R6</accession>
    <name>A0A452H7R6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H7R6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010582"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012144.1">
        <property type="protein sequence ID" value="ENSGAGP00000010582.1"/>
        <property type="gene ID" value="ENSGAGG00000008267.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20529">
        <property type="entry name" value="CYCLIN_CCNJ-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="7"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="4">
        <location>
            <begin position="158"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="162"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="387" mass="43433" checksum="212F118EC61FDEB9" modified="2019-05-08" version="1">MRRYFTDLLTVLSSRCNLCPTARHLAIYLLDLFMDHYDITVKQLHVISIACLLLASKRRLLLVLVGSLPPALPRLHSCPVPIPTPSPSPCPGPASSPSPPLSVPCSRKFEEKEDRVPKLEQLNNLAYMCNVNVVLNKKDLLKMELLLLENFNWNLCLPTPAHYIDYYLFASVVNGDRSERNTILKISLDYAFLNFRPSLIAAACVCASRICMQISPSWTTQLQLLTCYSWEHLAQCIEMMLIYYEKDVKEASKVKKQITLQQQVVGSVNHQTTTQVLFQQSSYHPLAQHSTALSQFQSPVQDLCSAYRDSLQAHRPSSLLSGSASTSLHSYSALQASLQPSVQTLPIQGPITMQVAIATEPRHCISMAYGSSYFSGHHTYTPRCFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HA96</accession>
    <name>A0A452HA96_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="5">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452HA96"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011644"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013339.1">
        <property type="protein sequence ID" value="ENSGAGP00000011644.1"/>
        <property type="gene ID" value="ENSGAGG00000008988.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000013340.1">
        <property type="protein sequence ID" value="ENSGAGP00000011645.1"/>
        <property type="gene ID" value="ENSGAGG00000008988.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050811">
        <property type="term" value="F:GABA receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032228">
        <property type="term" value="P:regulation of synaptic transmission, GABAergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16223">
        <property type="entry name" value="EFh_PRIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13364">
        <property type="entry name" value="PH_PLC_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08597">
        <property type="entry name" value="PI-PLCc_PRIP_metazoa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000005">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000025">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000017">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000001">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015359">
        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028382">
        <property type="entry name" value="PLCL2_EFh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF84">
        <property type="entry name" value="INACTIVE PHOSPHOLIPASE C-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="136"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="9">
        <location>
            <begin position="613"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="729"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1068" mass="119635" checksum="68D1ACCFA6DF8A64" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCP4</accession>
    <name>A0A452HCP4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Molybdate-anion transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Major facilitator superfamily domain-containing protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Molybdate transporter 2 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Mediates high-affinity intracellular uptake of the rare oligo-element molybdenum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HCP4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012552"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014386.1">
        <property type="protein sequence ID" value="ENSGAGP00000012552.1"/>
        <property type="gene ID" value="ENSGAGG00000009640.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015098">
        <property type="term" value="F:molybdate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17487">
        <property type="entry name" value="MFS_MFSD5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008509">
        <property type="entry name" value="MOT2/MFSD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23516:SF1">
        <property type="entry name" value="MOLYBDATE-ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23516">
        <property type="entry name" value="SAM (S-ADENOSYL METHIONINE) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05631">
        <property type="entry name" value="MFS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="408"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="452" mass="50863" checksum="8ECF33670E7605A4" modified="2019-05-08" version="1">MLFTAYVAFAVLLAICMGLEFSACRAKLAGSTCTNPAFLQFQLDYYQVYFLALVADWLQGPYLYKLYQHYHFLEGQIAIIYVCGFASSVLFGLVSTSLVDWLGRKKSCILFSLTYSICCLTKLSWDYFVLVVGRILGGLSTALLFSAFEAWYVHEHVERHDFPAEWIPATFSRAAFWNNVIAIGAGVTANFFAEWLGLGPVAPFMVSIPFLMLTGIFAMKNWDENYGKKRALSKTCVNGLKCLLSDRRVLLLGTIQALFESVIYIFIFLWTPVLDPYNPSLGIVFSSFMAASMVGSSLYRIATSKRYHLQPMHILSLSVLMVFFSLFMLTFSTNPGQENPSESFLAFLLIELSCGLYFPAMGFLRRKVIPEKDQVGVMNWFRMPLNLLACLGMLILHDSDYKTGTRNMFTICSVMMLMALLAVVSLFTVVRNNAELRVPSPQGHNELSSPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDS1</accession>
    <name>A0A452HDS1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HDS1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012991"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014886.1">
        <property type="protein sequence ID" value="ENSGAGP00000012991.1"/>
        <property type="gene ID" value="ENSGAGG00000009974.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990589">
        <property type="term" value="C:ATF4-CREB1 transcription factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14690">
        <property type="entry name" value="bZIP_CREB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000003">
        <property type="entry name" value="cAMP-responsive element modulator isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003102">
        <property type="entry name" value="CREB1-like_pKID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001630">
        <property type="entry name" value="Leuzip_CREB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45879">
        <property type="entry name" value="CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45879:SF2">
        <property type="entry name" value="CYCLIC AMP-DEPENDENT TRANSCRIPTION FACTOR ATF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02173">
        <property type="entry name" value="pKID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00041">
        <property type="entry name" value="LEUZIPPRCREB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50953">
        <property type="entry name" value="KID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="KID" evidence="9">
        <location>
            <begin position="79"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="260"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="80"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="278"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="318" mass="34495" checksum="406EBE1436B09C27" modified="2019-05-08" version="1">MEEVHKSTSNSSATPPQTTSIQGTPLQAAHLSHIAQQMSLRGPTPLTVVQLPGEQVQVQGVIQTAQSSVIHSPQVQTVQVSSLSESEDSQDSSDSIGSSQKTRGILARRPSYRKILKDLSSEDARDRKGDDESPGVSTVTSMSVPTPIYQTSTGQYITIAPNGALQLASSGTDGVQGLQTLTMTNAGGTQPGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPTTTSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYCHKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEB2</accession>
    <name>A0A452HEB2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HEB2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013145"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015050.1">
        <property type="protein sequence ID" value="ENSGAGP00000013145.1"/>
        <property type="gene ID" value="ENSGAGG00000010077.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097539">
        <property type="term" value="C:ciliary transition fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033545">
        <property type="entry name" value="CEP89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36170">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 89 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36170:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 89 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="451"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="693"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="801" mass="91929" checksum="222CF3444FF7AFA8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFH7</accession>
    <name>A0A452HFH7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HFH7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013595"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015561.1">
        <property type="protein sequence ID" value="ENSGAGP00000013595.1"/>
        <property type="gene ID" value="ENSGAGG00000010376.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902570">
        <property type="term" value="P:protein localization to nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48029">
        <property type="entry name" value="NUCLEOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48029:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="8"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="79" mass="9020" checksum="BFA0C6A9103DF753" modified="2019-05-08" version="1">MEKKQVLKRLYVGGLGHTVSEAELQERFSKFGNVTDAEIVIRKDDQGNPMKTFAYINISISEADLKRCKYTNYICCSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFZ3</accession>
    <name>A0A452HFZ3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FNBP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HFZ3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013769"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015764.1">
        <property type="protein sequence ID" value="ENSGAGP00000013766.1"/>
        <property type="gene ID" value="ENSGAGG00000010494.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000015767.1">
        <property type="protein sequence ID" value="ENSGAGP00000013769.1"/>
        <property type="gene ID" value="ENSGAGG00000010494.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000015770.1">
        <property type="protein sequence ID" value="ENSGAGP00000013772.1"/>
        <property type="gene ID" value="ENSGAGG00000010494.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11628">
        <property type="entry name" value="HR1_CIP4_FNBP1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11911">
        <property type="entry name" value="SH3_CIP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000002">
        <property type="entry name" value="Formin-binding protein 1-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000017">
        <property type="entry name" value="Formin-binding protein 1-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057871">
        <property type="entry name" value="HR1_CIP4_FNBP1L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057870">
        <property type="entry name" value="HR1_TOCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF17">
        <property type="entry name" value="CDC42-INTERACTING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25610">
        <property type="entry name" value="HR1_TOCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 16 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="20">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="21">
        <location>
            <begin position="373"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="517"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="256"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="474"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="82"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="308"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="481"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="578" mass="66451" checksum="7A8F02D571F9F338" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHS2</accession>
    <name>A0A452HHS2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Ephrin type-A receptor 6</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">EPH homology kinase 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts (via SAM domain) with ANKS1A (via SAM domain).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A452HHS2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014432"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016509.1">
        <property type="protein sequence ID" value="ENSGAGP00000014432.1"/>
        <property type="gene ID" value="ENSGAGG00000010972.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05066">
        <property type="entry name" value="PTKc_EphR_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000083">
        <property type="entry name" value="Ephrin type-A receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000190">
        <property type="entry name" value="Ephrin type-A receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1770">
        <property type="entry name" value="ephrin a2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001090">
        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050449">
        <property type="entry name" value="Ephrin_rcpt_TKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016257">
        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001426">
        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF10">
        <property type="entry name" value="EPHRIN TYPE-A RECEPTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14575">
        <property type="entry name" value="EphA2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25599">
        <property type="entry name" value="Ephrin_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01404">
        <property type="entry name" value="Ephrin_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000666">
        <property type="entry name" value="TyrPK_ephrin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51550">
        <property type="entry name" value="EPH_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00790">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00791">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 22 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 22 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="502"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Eph LBD" evidence="29">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="28">
        <location>
            <begin position="269"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="28">
        <location>
            <begin position="383"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="585"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="27">
        <location>
            <begin position="918"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="21">
        <location>
            <position position="751"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <begin position="591"/>
            <end position="599"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22 24">
        <location>
            <position position="617"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="14"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00065191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00077207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="989" mass="111299" checksum="4B6BD055808B21D0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJF4</accession>
    <name>A0A452HJF4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">General transcription factor IIH subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the GTF2H2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HJF4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015041"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017187.1">
        <property type="protein sequence ID" value="ENSGAGP00000015041.1"/>
        <property type="gene ID" value="ENSGAGG00000011370.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000017192.1">
        <property type="protein sequence ID" value="ENSGAGP00000015046.1"/>
        <property type="gene ID" value="ENSGAGG00000011370.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01453">
        <property type="entry name" value="vWA_transcription_factor_IIH_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000282">
        <property type="entry name" value="General transcription factor IIH subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000015">
        <property type="entry name" value="General transcription factor IIH subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007198">
        <property type="entry name" value="Ssl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004595">
        <property type="entry name" value="TFIIH_C1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012170">
        <property type="entry name" value="TFIIH_SSL1/p44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00622">
        <property type="entry name" value="ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07975">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04056">
        <property type="entry name" value="Ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015919">
        <property type="entry name" value="TFIIH_SSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01047">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="60"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="13">
        <location>
            <begin position="291"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR015919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR015919-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="395" mass="44757" checksum="3AC2D0A906EB1EFC" modified="2019-05-08" version="1">MDDEPERTKRWEGGYERTWEVLKEDESGSLKATIEDILFKAKRKRIFEHHGQVRLGMMRHLYVVVDGSRTMEDQDLKPNRLTCTLKLLEYFVEEYFDQNPISQIGIIVTKSKRAEKLTELSGNPRKHITALEKAVDLTCHGEPSLYNSLNLAMQTLKHMPGHTSREVLIIFSSLTTCDPSNIYDLIKCLKAVKIRVSVIGLSAEVRVCTVLARETGGMYHVILDESHYKELLMHHISPPPASSSSECSLIRMGFPQHTIASLSDQDAKPSFSMAHLENNSDPGLTLGGYFCPQCRAKYCELPVECKICGLTLVSAPHLARSYHHLFPLDAFQEVTLEEYKGERYCQGCQGEIKDQHVYICKVCQNVFCVECDLFVHDSLHCCPGCIHKHPTPLCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKF4</accession>
    <name>A0A452HKF4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing regulator WTAP</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Female-lethal(2)D homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">WT1-associated protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Wilms tumor 1-associating protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fl(2)d family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HKF4"/>
    <dbReference type="SMR" id="A0A452HKF4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015402"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017596.1">
        <property type="protein sequence ID" value="ENSGAGP00000015402.1"/>
        <property type="gene ID" value="ENSGAGG00000011625.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016556">
        <property type="term" value="P:mRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033757">
        <property type="entry name" value="WTAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15217:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING REGULATOR WTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15217">
        <property type="entry name" value="WILMS' TUMOR 1-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17098">
        <property type="entry name" value="Wtap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="236"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="335"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="55"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="277"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="335"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="353"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="371"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="397" mass="44525" checksum="B8DF37A23616AF98" modified="2019-05-08" version="1">MTNEEPLPKKVRLSETDFKVLGRDELILRWKQYEAYVQALEGKYTDLNSNDVTGLRESEEKLKQQQQESARRENILVMRLATKEQEMQECTNQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQLKETRQQLAQFQQQQQSQASAPGTSRTPSSEPTDQGEAMGKDCSRLANGPSNGSSSHQRTSGPGFYREGSGTEDDFPASPGNGNKLSNHSEDRTVRGGCSYINQLSTGYESVDSPTGSENSLTHHSNDTDSNHDPQEEKTVSMKGNRTVGSRHVQNGLDSTVNVQGSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HKM4</accession>
    <name>A0A452HKM4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Groucho/TLE N-terminal Q-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat Groucho/TLE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HKM4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015485"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017689.1">
        <property type="protein sequence ID" value="ENSGAGP00000015485.1"/>
        <property type="gene ID" value="ENSGAGG00000011690.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000017694.1">
        <property type="protein sequence ID" value="ENSGAGP00000015490.1"/>
        <property type="gene ID" value="ENSGAGG00000011690.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000001">
        <property type="entry name" value="transducin-like enhancer protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005617">
        <property type="entry name" value="Groucho/TLE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009146">
        <property type="entry name" value="Groucho_enhance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814">
        <property type="entry name" value="TRANSDUCIN-LIKE ENHANCER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814:SF24">
        <property type="entry name" value="TRANSDUCIN-LIKE ENHANCER PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03920">
        <property type="entry name" value="TLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01850">
        <property type="entry name" value="GROUCHOFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Groucho/TLE N-terminal Q-rich" evidence="10">
        <location>
            <begin position="18"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="576"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="618"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="137"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="196"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="219"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="294"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="776" mass="83909" checksum="3997AA5AB5019998" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HL39</accession>
    <name>A0A452HL39_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycerol-3-phosphate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="11">1.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>sn-glycerol 3-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:11093"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.8" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A452HL39"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015650"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017876.1">
        <property type="protein sequence ID" value="ENSGAGP00000015650.1"/>
        <property type="gene ID" value="ENSGAGG00000011806.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141152">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000088">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000084">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017751">
        <property type="entry name" value="G3P_DH_NAD-dep_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03376">
        <property type="entry name" value="glycerol3P_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728:SF7">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9 10" id="KW-0520">NAD</keyword>
    <keyword evidence="4 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal" evidence="12">
        <location>
            <begin position="66"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal" evidence="13">
        <location>
            <begin position="253"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="70"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="329"/>
            <end position="330"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="409" mass="44166" checksum="110F81A962A71D5D" modified="2019-05-08" version="1">MDYSSQQAAGAAAAACERLRAGGVRRVFSPVRGLEALAAASLAETRCRGAGGAAPAMALVSAPLRVCIVGSGNWGSAVAKIIGNNVKNMQKFASTVKMWVFEENINGRKLTDIINNEHENVKYLPGYKLPENVVAIPNLNEAVQDADLLVFVIPHQFIHKICDEITGRVPKKAVGITLIKGIDEGPEGLKLISDIIREKMGIDISVLMGANIANEVAAEKFCETTIGSKILENGLLFKELLQTPNFRITVVDDADTVELCGALKNIVAVGAGFCDGLRCGDNTKAAVIRLGLMEMIAFAKIFCKGQVSIATFLESCGVADLITTCYGGRNRRVAEAFVKTGKSIEELEQEMLNGQKLQGPQTSAEVYRILKQKGMVDKFPLFTAVYQICYEGKPVQEMISCLQSHPEHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQY4</accession>
    <name>A0A452HQY4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">CCR4-NOT transcription complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">CCR4-associated factor 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="15">Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT2. Interacts with TIP120B and NANOS2. Interacts with EBF1. Interacts in an RNA-independent manner with BICC1 (via KH domains).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HQY4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017428"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019879.1">
        <property type="protein sequence ID" value="ENSGAGP00000017428.1"/>
        <property type="gene ID" value="ENSGAGG00000012677.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000036">
        <property type="term" value="P:regulation of stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1020:FF:000002">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012270">
        <property type="entry name" value="CCR4-NOT_su3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007207">
        <property type="entry name" value="Not_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04065">
        <property type="entry name" value="Not3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005290">
        <property type="entry name" value="NOT_su_3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="11 16" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="CCR4-Not complex component Not N-terminal" evidence="18">
        <location>
            <begin position="10"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="19">
        <location>
            <begin position="578"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="243"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="298"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="419"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="259"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="298"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="325"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="457"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="709" mass="78401" checksum="6318ABBAFF86E48A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HR15</accession>
    <name>A0A452HR15_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosome assembly factor mrt4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 8">Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associates with the pre-60S ribosomal particle. Interacts with MINAS-60 (product of an alternative open reading frame of RBM10).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
            <location evidence="8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HR15"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017497"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019954.1">
        <property type="protein sequence ID" value="ENSGAGP00000017497.1"/>
        <property type="gene ID" value="ENSGAGG00000012997.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05796">
        <property type="entry name" value="Ribosomal_P0_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000004">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000002">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033867">
        <property type="entry name" value="Mrt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="9">
        <location>
            <begin position="125"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="238" mass="27622" checksum="7A5566C82E03628A" modified="2019-05-08" version="1">MLKLFCFLSVSLTKTTKKGLEVKQSLIEELRKCVDTYKYLFIFSVVNMRNNKLKDIRSAWKHSRIFFGKNKVMMVALGREPADEYKENLHQVSKHLRGEVGLLFTNRTKEEVNEWFSQFKEVDFARAGNKATFTVSLDTGPLEQFPHSMEPQLRQLGLPTTLKKGVVTLLSDYEVCKEGDVLTPEQARVLKLFGYEMAEFKVTIKCMWYSETGDFQQLVKEAEETSEMEDDDEEENDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRS8</accession>
    <name>A0A452HRS8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="36">Tyrosine-protein kinase receptor</fullName>
            <ecNumber evidence="36">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="42 43">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="43" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="42" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="42" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome membrane</location>
            <topology evidence="4">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="30">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="36">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="36"/>
    <dbReference type="AlphaFoldDB" id="A0A452HRS8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017767"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020267.1">
        <property type="protein sequence ID" value="ENSGAGP00000017767.1"/>
        <property type="gene ID" value="ENSGAGG00000013192.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048403">
        <property type="term" value="F:brain-derived neurotrophic factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060175">
        <property type="term" value="F:brain-derived neurotrophic factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990416">
        <property type="term" value="P:cellular response to brain-derived neurotrophic factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099551">
        <property type="term" value="P:trans-synaptic signaling by neuropeptide, modulating synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05855">
        <property type="entry name" value="IgI_TrkB_d5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05093">
        <property type="entry name" value="PTKc_TrkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000239">
        <property type="entry name" value="BDNF/NT-3 growth factors receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000034">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000486">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000033">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000319">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020777">
        <property type="entry name" value="NTRK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020455">
        <property type="entry name" value="NTRK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031635">
        <property type="entry name" value="NTRK_LRRCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002011">
        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF136">
        <property type="entry name" value="BDNF_NT-3 GROWTH FACTORS RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16920">
        <property type="entry name" value="LRRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01939">
        <property type="entry name" value="NTKRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01941">
        <property type="entry name" value="NTKRECEPTOR2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00239">
        <property type="entry name" value="RECEPTOR_TYR_KIN_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19 33 35" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="28" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="18" id="KW-0221">Differentiation</keyword>
    <keyword evidence="25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0967">Endosome</keyword>
    <keyword evidence="27" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="29" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="17" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="23 38" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="15 33 35" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 36" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26 36" id="KW-0675">Receptor</keyword>
    <keyword evidence="43" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 39" id="KW-0732">Signal</keyword>
    <keyword evidence="22" id="KW-0770">Synapse</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 36 38" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="21 38" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="24" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="39">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019232538" description="Tyrosine-protein kinase receptor" evidence="39">
        <location>
            <begin position="33"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="38">
        <location>
            <begin position="429"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="41">
        <location>
            <begin position="198"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="40">
        <location>
            <begin position="536"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="37">
        <location>
            <begin position="471"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="37">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="32">
        <location>
            <position position="674"/>
        </location>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <begin position="542"/>
            <end position="550"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33 35">
        <location>
            <position position="570"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with SHC1" evidence="34">
        <location>
            <position position="514"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with PLCG1" evidence="34">
        <location>
            <position position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="PIRSR" id="PIRSR620777-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="PIRSR" id="PIRSR620777-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="PIRSR" id="PIRSR620777-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="RuleBase" id="RU000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="40">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="41">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="42">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="43">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="820" mass="92000" checksum="AF2DAA9C2E168E50" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWM3</accession>
    <name>A0A452HWM3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prostaglandin F2-alpha receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Prostanoid FP receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HWM3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019485"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022188.1">
        <property type="protein sequence ID" value="ENSGAGP00000019485.1"/>
        <property type="gene ID" value="ENSGAGG00000014368.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004958">
        <property type="term" value="F:prostaglandin F receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15145">
        <property type="entry name" value="7tmA_FP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000129">
        <property type="entry name" value="Prostaglandin F2-alpha receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000141">
        <property type="entry name" value="PglndnF_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF4">
        <property type="entry name" value="PROSTAGLANDIN F2-ALPHA RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00855">
        <property type="entry name" value="PRSTNOIDFPR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="195"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="243"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="38"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="361" mass="40681" checksum="2EDEDE702F7B75F3" modified="2019-05-08" version="1">MNSTKSPVLDGLGNTTCNAHNKISVFFSIIFMTVGILSNSLAIAILMKAYQRFRQKSKASFLLFASGLVVTDLFGHLINGTIAVFVYASDKDWIRFNQSNILCSVFGICMVFFGLCPLFLGSVMAVERCIGVTKPIFHSTKMTSKHVKMILTTVCLFAVFIALLPILSFRAYQIQASRTWCFYKTEHIEDWEDRFYLLLFSCLGLLALAVSFLCNAVTGITLLRVKFKSQQRQGRSHHFEMIIQLLAIMCVSCICWSPFLVTMANIGINADRSMETCETILVVLRMATWNQILDPWVYILLRKAVLKTLYKIARRCCGMHIMNLHMWELSSIKNSLKVAAISESPVSSKQINQQPLSSTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXP9</accession>
    <name>A0A452HXP9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HXP9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020003"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022787.1">
        <property type="protein sequence ID" value="ENSGAGP00000020003.1"/>
        <property type="gene ID" value="ENSGAGG00000014739.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15961">
        <property type="entry name" value="7tmA_GPR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000067">
        <property type="entry name" value="G-protein coupled receptor 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000599">
        <property type="entry name" value="GPR12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000723">
        <property type="entry name" value="GPR_3/6/12_orphan"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00650">
        <property type="entry name" value="GPR12ORPHANR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00644">
        <property type="entry name" value="GPRORPHANR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="149"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="130"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="403" mass="43981" checksum="D6B13A1CA0D02142" modified="2019-05-08" version="1">MHQAQRPGASAEGIAPALPTAQPGAGTAAPSPCLCSANNEPARSSLLCCIERTWRLSSSSRGEAKTGVKMNEDLKVNLSWLPQDHLEASSPENVSTAVSSLIPVVDPEPELIVNPWDIVLCTSGTLISCENAIVVLIIFQNPNLRAPMFLLIGSLALADLLAGIGLIINFIFAYLLQSEATKLVTIGLIVASFSASVCSLLAITVDRYLSLYYALTYNSERTVTFTYIMLILLWGASICIGLLPIMGWNCLKDESTCSVIRPLTKNNAAILSVSFLLMFALMLQLYIQICKIVMRHAHQIALQHHFLATSHYVTTRKGVSTLAIILGTFAACWMPFTLYSLIADYTYPSIYTYATLLPATYNSIINPVIYAFRNQEIQKAFWLICCGCIPSSLSQRARSPSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXR1</accession>
    <name>A0A452HXR1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000019980"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022764.1">
        <property type="protein sequence ID" value="ENSGAGP00000019980.1"/>
        <property type="gene ID" value="ENSGAGG00000014720.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045180">
        <property type="term" value="C:basal cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14673">
        <property type="entry name" value="PH_PHLDB1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22265">
        <property type="entry name" value="UDM1_RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000006">
        <property type="entry name" value="Pleckstrin homology like domain family B member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052212">
        <property type="entry name" value="PH-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037810">
        <property type="entry name" value="PHLDB1/2/3_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12156:SF21">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN FAMILY B MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12156">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN, FAMILY B, MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="1257"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="307"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="880"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="955"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1043"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="625"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1167"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="880"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="901"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="976"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1002"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1050"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1061"/>
            <end position="1072"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1367" mass="155559" checksum="C9745DD53C69FFBD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1E7</accession>
    <name>A0A452I1E7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="18">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endomembrane system</location>
            <topology evidence="14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A452I1E7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021284"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024236.1">
        <property type="protein sequence ID" value="ENSGAGP00000021284.1"/>
        <property type="gene ID" value="ENSGAGG00000015624.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000002">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF31">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE 1,2-ALPHA-MANNOSIDASE IA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 18" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="521"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="546"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="632"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="475"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="652" mass="72565" checksum="F86B57A10CF502C3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2L8</accession>
    <name>A0A452I2L8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Microtubule associated protein 1B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000021755"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024780.1">
        <property type="protein sequence ID" value="ENSGAGP00000021755.1"/>
        <property type="gene ID" value="ENSGAGG00000015963.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031114">
        <property type="term" value="P:regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026074">
        <property type="entry name" value="MAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057480">
        <property type="entry name" value="MAP1A/B/S-like_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056617">
        <property type="entry name" value="MAP1B/S_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13843">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13843:SF5">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23415">
        <property type="entry name" value="MAPB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25281">
        <property type="entry name" value="MBL_MAP1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Microtubule-associated protein 1B/S N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Microtubule-associated protein 1A/B/S-like MBL-like" evidence="3">
        <location>
            <begin position="114"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="726"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="940"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1054"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1120"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1201"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1303"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1431"/>
            <end position="1694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1718"/>
            <end position="1739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1752"/>
            <end position="1816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1847"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1884"/>
            <end position="1968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2039"/>
            <end position="2296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="766"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="779"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="789"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="841"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="884"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="986"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1065"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1077"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1219"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1303"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1468"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1520"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1600"/>
            <end position="1622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1670"/>
            <end position="1683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1722"/>
            <end position="1739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1767"/>
            <end position="1777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1784"/>
            <end position="1794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2081"/>
            <end position="2106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2224"/>
            <end position="2245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2254"/>
            <end position="2266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2267"/>
            <end position="2285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2286"/>
            <end position="2295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2413" mass="266746" checksum="F15A6A9BD3C794ED" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I2U8</accession>
    <name>A0A452I2U8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I2U8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021826"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024863.1">
        <property type="protein sequence ID" value="ENSGAGP00000021826.1"/>
        <property type="gene ID" value="ENSGAGG00000016021.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039964">
        <property type="entry name" value="KIAA0040-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40382">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40382:SF1">
        <property type="entry name" value="RIKEN CDNA 4930523C07 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="138" mass="15620" checksum="37562D749C9218F0" modified="2019-05-08" version="1">MERVSSFFDSLWNLIHTKHQEGIFNTVCLVVLLGLPFLVLLVLLVICFHCCFCRRRGKGSSNGSSSNGQVPAERKKRRKKKKKGEEDLWISAQPKPESKYTSYLLSTKASLPPRPALRDIVPNRTPHTHFTDVSSSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I411</accession>
    <name>A0A452I411_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I411"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022254"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025364.1">
        <property type="protein sequence ID" value="ENSGAGP00000022254.1"/>
        <property type="gene ID" value="ENSGAGG00000016346.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="386"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="442"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="618" mass="66435" checksum="E4DE34AE34703B8E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4I7</accession>
    <name>A0A452I4I7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I4I7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022472"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025601.1">
        <property type="protein sequence ID" value="ENSGAGP00000022472.1"/>
        <property type="gene ID" value="ENSGAGG00000016481.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="182" mass="19353" checksum="7C4A90AD4A3B2305" modified="2019-05-08" version="1">MANSGLQLLGFVLALLGWVALIAATILPQWRMSSYAGDNIITSVAVYQGLWMSCAWQSTGQIQCKAYDSILNLSSALQATRALMVVSIVMGIIGIGVASMGMKCTRCGGDNKVQKARIAMTGGLIFVVGGESRKGLAGGLPLGSSMYLGLEWVGLLGRLAETWAIHFMHEISWETAPARGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4V0</accession>
    <name>A0A452I4V0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CD3e molecule associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I4V0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022542"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025682.1">
        <property type="protein sequence ID" value="ENSGAGP00000022542.1"/>
        <property type="gene ID" value="ENSGAGG00000016535.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013240">
        <property type="entry name" value="DNA-dir_RNA_pol1_su_RPA34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15484">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15484:SF8">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08208">
        <property type="entry name" value="RNA_polI_A34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="29740" checksum="3BBA21A8FBF44572" modified="2019-05-08" version="1">MDRAALRGLPRFQCPPDFSPSPFRPGTPFAPEELRGKELWLIRAPADFSPDSLNGCAVPLVGFQTLKTKFDGTQQVFDIHSALEESGSPYLLVSSTHSDQLSCAASFHGCMRICERFGDPSSRSPGQAVRARPAPQVPEGLRQRFLPFGSSPKQQCPEAATSTPATDVLGSARKKKKKKRVKEEPMELLVSIKQEPPEEPWGWGSGDPGPEPPRAEDDGLRGAEEPTLGHLSPKHKKKKKKKKKHKYEALEGAVWPCKAERLDPSSIKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4W1</accession>
    <name>A0A452I4W1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I4W1"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025698.1">
        <property type="protein sequence ID" value="ENSGAGP00000022556.1"/>
        <property type="gene ID" value="ENSGAGG00000016547.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="39" mass="4244" checksum="29EA244B126C5563" modified="2019-05-08" version="1">MNKALLKRELGTSVLQSPGHSGGHCSSQSLKYDAEQTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I563</accession>
    <name>A0A452I563_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GA-binding protein alpha chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I563"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022688"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025844.1">
        <property type="protein sequence ID" value="ENSGAGP00000022688.1"/>
        <property type="gene ID" value="ENSGAGG00000016634.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08534">
        <property type="entry name" value="SAM_PNT-GABP-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000110">
        <property type="entry name" value="GA-binding protein alpha chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000200">
        <property type="entry name" value="GA-binding protein alpha chain, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000039">
        <property type="entry name" value="GA-binding protein alpha chain, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024668">
        <property type="entry name" value="GABP_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003118">
        <property type="entry name" value="Pointed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016312">
        <property type="entry name" value="TF_GA-bd_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF195">
        <property type="entry name" value="GA-BINDING PROTEIN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11620">
        <property type="entry name" value="GABP-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02198">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001703">
        <property type="entry name" value="GABP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00251">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51433">
        <property type="entry name" value="PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PNT" evidence="13">
        <location>
            <begin position="168"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="ETS" evidence="12">
        <location>
            <begin position="321"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="455" mass="51351" checksum="28D80434BC945340" modified="2019-05-08" version="1">MTKRDAEELIEIEIDGTEKQECTEESIVEQTYTTAEFVSQAIDINEPVGNLKKLLEPRLQCSLDAHEICLQDIQLDPDRSLFDQGVKTDGTVQLSVQVISRQGIEPKLNILEIVKPVETVEVVIDPDAHHAEAEAHLVEEAQVITLDGTKHITTISDETSEQVTRWAAALEGYRKEQERLGIPYDPVQWSTDQVLHWVVWVMKEFSMTDIDLNTLSIPGRELCSLNQEDFFQRVPRGEILWSHLELLRKYVLASQEHSGEIATVTIDQPVQIIPASVQPATPTTIKVINSSAKAAKVQRAPRISGEDRSSPGNRTGNNGQIQLWQFLLELLTDKDARDCISWVGDEGEFKLNQPELVAQKWGQRKNKPTMNYEKLSRALRYYYDGDMICKVQGKRFVYKFVCDLKTLIGYSAAELNRLVTECEQKKLAKMQLHGIAQPVTAVALATASLQTEKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6M7</accession>
    <name>A0A452I6M7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Zinc finger homeobox protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Zinc finger homeodomain protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">May play a role in neural and muscle differentiation. May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18 19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000023247"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026484.1">
        <property type="protein sequence ID" value="ENSGAGP00000023247.1"/>
        <property type="gene ID" value="ENSGAGG00000017044.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000082">
        <property type="entry name" value="Putative zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000317">
        <property type="entry name" value="zinc finger homeobox protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000429">
        <property type="entry name" value="Zinc finger homeobox protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000058">
        <property type="entry name" value="zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000064">
        <property type="entry name" value="Zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000096">
        <property type="entry name" value="Zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000081">
        <property type="entry name" value="Zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001440">
        <property type="entry name" value="Zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051968">
        <property type="entry name" value="ZnFinger_Homeobox_TR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45891">
        <property type="entry name" value="ZINC FINGER HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45891:SF2">
        <property type="entry name" value="ZINC FINGER HOMEOBOX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24056">
        <property type="entry name" value="zf-C2H2_ZFHX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 18 19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 18 19" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 18 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="1353"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="1381"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="1497"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="1549"/>
            <end position="1578"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="1700"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="1752"/>
            <end position="1781"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="2094"/>
            <end position="2122"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="21">
        <location>
            <begin position="2289"/>
            <end position="2349"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="21">
        <location>
            <begin position="2386"/>
            <end position="2446"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="2474"/>
            <end position="2503"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="21">
        <location>
            <begin position="2765"/>
            <end position="2825"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="2837"/>
            <end position="2865"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="21">
        <location>
            <begin position="3091"/>
            <end position="3151"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="18">
        <location>
            <begin position="2291"/>
            <end position="2350"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="18">
        <location>
            <begin position="2388"/>
            <end position="2447"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="18">
        <location>
            <begin position="2767"/>
            <end position="2826"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="18">
        <location>
            <begin position="3093"/>
            <end position="3152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="423"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="522"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="574"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1084"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1255"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1448"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1582"/>
            <end position="1607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1785"/>
            <end position="1810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1966"/>
            <end position="1985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2003"/>
            <end position="2051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2141"/>
            <end position="2232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2496"/>
            <end position="2515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2528"/>
            <end position="2642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2718"/>
            <end position="2772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2972"/>
            <end position="3013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="3039"/>
            <end position="3094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="3279"/>
            <end position="3388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="3498"/>
            <end position="3554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="3660"/>
            <end position="3679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="3729"/>
            <end position="3751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="423"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="434"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="468"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1106"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1299"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1456"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1586"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1789"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1966"/>
            <end position="1981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="2003"/>
            <end position="2038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="2148"/>
            <end position="2164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="2176"/>
            <end position="2189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="2190"/>
            <end position="2213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="2214"/>
            <end position="2226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="2500"/>
            <end position="2515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="2534"/>
            <end position="2553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="2559"/>
            <end position="2576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="2577"/>
            <end position="2600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="2601"/>
            <end position="2618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="2627"/>
            <end position="2642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="2718"/>
            <end position="2738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="2742"/>
            <end position="2766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="2972"/>
            <end position="2992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="3039"/>
            <end position="3048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="3303"/>
            <end position="3323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="3325"/>
            <end position="3341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="3370"/>
            <end position="3388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="3498"/>
            <end position="3516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="3532"/>
            <end position="3554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="3664"/>
            <end position="3679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00083806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="3784" mass="419204" checksum="7B0C6349C2CAE143" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I6T5</accession>
    <name>A0A452I6T5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nematocyst</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I6T5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023311"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026552.1">
        <property type="protein sequence ID" value="ENSGAGP00000023311.1"/>
        <property type="gene ID" value="ENSGAGG00000017088.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042151">
        <property type="term" value="C:nematocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044218">
        <property type="term" value="C:other organism cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051715">
        <property type="term" value="P:cytolysis in another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:monoatomic cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046931">
        <property type="term" value="P:pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.270.20">
        <property type="entry name" value="Cytolysin/lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050677">
        <property type="entry name" value="Actinoporin_PFT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009104">
        <property type="entry name" value="Anemon_actinoporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015926">
        <property type="entry name" value="Cytolysin/lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40388">
        <property type="entry name" value="BRYOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40388:SF1">
        <property type="entry name" value="BRYOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06369">
        <property type="entry name" value="Anemone_cytotox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63724">
        <property type="entry name" value="Cytolysin/lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0166">Nematocyst</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1052">Target cell membrane</keyword>
    <keyword evidence="4" id="KW-1053">Target membrane</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="172"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="203" mass="22388" checksum="92704211E074F476" modified="2019-05-08" version="1">MINVGELIAPMGAGRCVGIQITNNTRDVTLEYPRTYCFSGRAMIEPVSHIPPGSSGSSVFVKTSYTARGSVGVLSYESDVFTLAIMFSNPFDRVLYSSEFAIQIFTGRKHFHSMEHLYNYMYSNGPPYICKSYQKVKLEVTDGQLEVINEEIQVKASMSSKDKSIIQVQIEQKDPCPPYTAKPADPSPRKCPNVPDKGAAGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8R2</accession>
    <name>A0A452I8R2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Low-density lipoprotein receptor-related protein 12</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Probable receptor, which may be involved in the internalization of lipophilic molecules and/or signal transduction. May act as a tumor suppressor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">May interact with RACK1, ZFYVE9 and NMRK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Membrane</location>
            <location evidence="13">Coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I8R2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024057"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027399.1">
        <property type="protein sequence ID" value="ENSGAGP00000024057.1"/>
        <property type="gene ID" value="ENSGAGG00000017602.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000021">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000024">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000063">
        <property type="entry name" value="low-density lipoprotein receptor-related protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000040">
        <property type="entry name" value="low-density lipoprotein receptor-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000003">
        <property type="entry name" value="Putative low-density lipoprotein receptor-related protein 12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050685">
        <property type="entry name" value="LDLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270:SF47">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0168">Coated pit</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="CUB" evidence="19">
        <location>
            <begin position="93"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="19">
        <location>
            <begin position="304"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="670"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="734"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="794"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="736"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="231"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="284"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="440"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="478"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="496"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="503"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="515"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="904" mass="98795" checksum="9C158241089012F0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAM7</accession>
    <name>A0A452IAM7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aryl hydrocarbon receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IAM7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024762"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028177.1">
        <property type="protein sequence ID" value="ENSGAGP00000024762.1"/>
        <property type="gene ID" value="ENSGAGG00000018077.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034751">
        <property type="term" value="C:aryl hydrocarbon receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904613">
        <property type="term" value="P:cellular response to 2,3,7,8-tetrachlorodibenzodioxine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11436">
        <property type="entry name" value="bHLH-PAS_AhR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000035">
        <property type="entry name" value="Aryl hydrocarbon receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000019">
        <property type="entry name" value="Aryl hydrocarbon receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000024">
        <property type="entry name" value="Aryl hydrocarbon receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039091">
        <property type="entry name" value="AHR/AHRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033348">
        <property type="entry name" value="AHR_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10649">
        <property type="entry name" value="ARYL HYDROCARBON RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10649:SF18">
        <property type="entry name" value="ARYL HYDROCARBON RECEPTOR 1 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="9" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="16">
        <location>
            <begin position="19"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="15">
        <location>
            <begin position="113"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="419"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="600"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="607"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="986" mass="109787" checksum="1D0211C3F2B623A8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBD7</accession>
    <name>A0A452IBD7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IBD7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024831"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028252.1">
        <property type="protein sequence ID" value="ENSGAGP00000024831.1"/>
        <property type="gene ID" value="ENSGAGG00000018129.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="99" mass="11106" checksum="52346E788BC8F70B" modified="2019-05-08" version="1">MFPSLLVTVSCSGLIQSTLPQAQWDRMLYGTLQVGYFVILKTCGSVFYLILPCFTSQFLAAKDSLQPSEEKVGAFTKIIEAMGFTGPLKYNKWVTQLIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IBX9</accession>
    <name>A0A452IBX9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM171 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IBX9"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028687.1">
        <property type="protein sequence ID" value="ENSGAGP00000025198.1"/>
        <property type="gene ID" value="ENSGAGG00000018432.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043149">
        <property type="term" value="P:stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018890">
        <property type="entry name" value="FAM171"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049175">
        <property type="entry name" value="FAM171_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048530">
        <property type="entry name" value="FAM171_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31626:SF1">
        <property type="entry name" value="PROTEIN FAM171A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31626">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20771">
        <property type="entry name" value="FAM171A1-2-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10577">
        <property type="entry name" value="FAM171A1-2-B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019331003" evidence="10">
        <location>
            <begin position="22"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="FAM171 N-terminal" evidence="11">
        <location>
            <begin position="33"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="FAM171 C-terminal" evidence="12">
        <location>
            <begin position="396"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="419"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="633"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="734"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="823"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="425"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="442"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="633"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="751"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="862"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="875"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="895" mass="98974" checksum="7E6A5322895AB854" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IF88</accession>
    <name>A0A452IF88_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IF88"/>
    <dbReference type="SMR" id="A0A452IF88"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026602"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030240.1">
        <property type="protein sequence ID" value="ENSGAGP00000026602.1"/>
        <property type="gene ID" value="ENSGAGG00000019379.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000002">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="13">
        <location>
            <begin position="49"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="14">
        <location>
            <begin position="248"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="450" mass="49960" checksum="2A78714CBA782D55" modified="2019-05-08" version="1">MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGVDSVEAEAEEGEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IHW7</accession>
    <name>A0A452IHW7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Centromere protein F</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000027368"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031091.1">
        <property type="protein sequence ID" value="ENSGAGP00000027368.1"/>
        <property type="gene ID" value="ENSGAGG00000019901.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051310">
        <property type="term" value="P:metaphase chromosome alignment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043513">
        <property type="entry name" value="Cenp-F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018302">
        <property type="entry name" value="CenpF/LEK1_Rb-prot-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019513">
        <property type="entry name" value="Centromere_CenpF_leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018463">
        <property type="entry name" value="Centromere_CenpF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18874:SF10">
        <property type="entry name" value="CENTROMERE PROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18874">
        <property type="entry name" value="CMF/LEK/CENP CELL DIVISION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10490">
        <property type="entry name" value="CENP-F_C_Rb_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10473">
        <property type="entry name" value="CENP-F_leu_zip"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10481">
        <property type="entry name" value="CENP-F_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centromere protein Cenp-F N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere protein Cenp-F leucine-rich repeat-containing" evidence="3">
        <location>
            <begin position="1932"/>
            <end position="2068"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere protein Cenp-F leucine-rich repeat-containing" evidence="3">
        <location>
            <begin position="2170"/>
            <end position="2309"/>
        </location>
    </feature>
    <feature type="domain" description="Kinetochore protein Cenp-F/LEK1 Rb protein-binding" evidence="5">
        <location>
            <begin position="2771"/>
            <end position="2814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2702"/>
            <end position="2754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2901"/>
            <end position="2922"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="13"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="271"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="479"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="670"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="893"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1083"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1176"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1311"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1410"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1570"/>
            <end position="1680"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1824"/>
            <end position="1907"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1950"/>
            <end position="2117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2146"/>
            <end position="2324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2404"/>
            <end position="2431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2480"/>
            <end position="2696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2702"/>
            <end position="2719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2720"/>
            <end position="2729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2737"/>
            <end position="2748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="2913"/>
            <end position="2922"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2922" mass="339760" checksum="5DB0C7FED534B95A" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIU9</accession>
    <name>A0A452IIU9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IIU9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027813"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031598.1">
        <property type="protein sequence ID" value="ENSGAGP00000027813.1"/>
        <property type="gene ID" value="ENSGAGG00000020215.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19811">
        <property type="entry name" value="Bbox1_TRIM66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19794">
        <property type="entry name" value="Bbox2_TRIM66-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05502">
        <property type="entry name" value="Bromo_tif1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15625">
        <property type="entry name" value="PHD_TIF1delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000123">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037372">
        <property type="entry name" value="TRIM66_Bbox1_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915:SF7">
        <property type="entry name" value="TRIPARTITE MOTIF-CONTAINING PROTEIN 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25287">
        <property type="entry name" value="zf-B_box_Trim66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 11" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="10"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="16">
        <location>
            <begin position="87"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="16">
        <location>
            <begin position="147"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="14">
        <location>
            <begin position="1011"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="13">
        <location>
            <begin position="1101"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="478"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="674"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1199"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="677"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="718"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1259" mass="143034" checksum="A00E679E5EA8FDCC" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIV1</accession>
    <name>A0A452IIV1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IIV1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027761"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031537.1">
        <property type="protein sequence ID" value="ENSGAGP00000027761.1"/>
        <property type="gene ID" value="ENSGAGG00000020180.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052286">
        <property type="entry name" value="Wnt_signaling_inhibitor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24364">
        <property type="entry name" value="LP06937P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24364:SF17">
        <property type="entry name" value="TROPHOBLAST GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01463">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019507407" evidence="5">
        <location>
            <begin position="36"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="6">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="7">
        <location>
            <begin position="260"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="388" mass="41854" checksum="7479AFCA8399BA7F" modified="2019-05-08" version="1">MAGRALRGAAPPAPGGERRAALGLLLQVLLGWGSAQQPGSCPAPCECSEAARTVKCVNRNLTAVPPDLPPYARLLFLTGNRLASLPPGAFLSPPLPQLSLLNLSGSHVEQVEAGAFASLASLRQLDLSGNALTRFSPEAFGNASSPLEELNLSNSLSNQSAVAALAELLGSGVLGNLSRLELADNRLLSLPAGMFSSLPSLQHLDLRNNSLVSLQAATFHSLAQLQSLDLSHNSLKCLKNATIAQLRSRPVLRRVSLGHNTWVCDCCIEHLVSWLKESEQVEGKGALKCSYPEKMRDKPLVKTNSSDLDCSVPVDIQSQLQTSYVFLGIVLALIGAIFLLVLYLNRKGIKKWMYNIRDACRDHMEGYHYRYEVNADPRLPNLSSSSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IK81</accession>
    <name>A0A452IK81_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000028286"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032141.1">
        <property type="protein sequence ID" value="ENSGAGP00000028286.1"/>
        <property type="gene ID" value="ENSGAGG00000020531.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050265">
        <property type="term" value="F:RNA uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000002">
        <property type="entry name" value="terminal uridylyltransferase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000005">
        <property type="entry name" value="terminal uridylyltransferase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045100">
        <property type="entry name" value="TUT4/7_NTP_transf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF49">
        <property type="entry name" value="TERMINAL URIDYLYLTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19088">
        <property type="entry name" value="TUTase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="1242"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="1285"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="521"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="660"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1329"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="529"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="544"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="660"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1350"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1365"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1385"/>
            <end position="1397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1594" mass="181144" checksum="90CC98AB7A605C93" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IKL4</accession>
    <name>A0A452IKL4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5-hydroxytryptamine receptor 2B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Serotonin receptor 2B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Synapse</location>
            <location evidence="18">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IKL4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028515"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032385.1">
        <property type="protein sequence ID" value="ENSGAGP00000028515.1"/>
        <property type="gene ID" value="ENSGAGG00000020659.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051378">
        <property type="term" value="F:serotonin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007208">
        <property type="term" value="P:phospholipase C-activating serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050795">
        <property type="term" value="P:regulation of behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007210">
        <property type="term" value="P:serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006939">
        <property type="term" value="P:smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042310">
        <property type="term" value="P:vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15306">
        <property type="entry name" value="7tmA_5-HT2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000523">
        <property type="entry name" value="5-hydroxytryptamine receptor 2B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000482">
        <property type="entry name" value="5HT2B_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF31">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00651">
        <property type="entry name" value="5HT2BRECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0085">Behavior</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 19" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="15 19" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 19 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="21">
        <location>
            <begin position="70"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="479" mass="53869" checksum="C3A9E4C4D067DC6A" modified="2019-05-08" version="1">MSEQNTIPEYILLGTKMSYPLNGFGFGNGSLTQLSVAGEFKQNYLNDEQGNRPRWAALLILMVIIPTIGGNILVILAVSLEKKLQYATNYFLMSLAVADLLVGLFVMPIALIIILFDNAWPLPPDLCPVWLFLDVLFSTASIMHLCAISLDRYIAIKKPIQASQYNSRATALIKITVVWLISIGIAIPIPIRGIEDGDGSSTNITCALTPDRFRDFILYGSLAAFFVPLAIMIVTYFLTIQELRKKAYLINKPPQRLTWSTVSTVFQRDMTPSSSPEKVAMLDGSRKDKVMPSTNEEIFIRRMSTAGKKSVQTISNEQRASKVLGIVFFLFLLMWCPFFITNITSVLCDSCSQEVIQRLMEIFVWIGYVSSGVNPLVYTLFNKTFREAFGRYITCNYRTAKPMKALRKCSSRISFRNSMAENSKLFMMHGMRNGISPVMYQSPMRLHSSPIQSSSAILLDTLLLTENEVDKTEEQVSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IL31</accession>
    <name>A0A452IL31_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ras-related protein Rab-25</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB25 regulates epithelial cell differentiation, proliferation and survival, thereby playing key roles in tumorigenesis. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts (GTP-bound form) with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP-dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer. Interacts with VPS33B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Pseudopodium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IL31"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028578"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032457.1">
        <property type="protein sequence ID" value="ENSGAGP00000028578.1"/>
        <property type="gene ID" value="ENSGAGG00000020703.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031260">
        <property type="term" value="C:pseudopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="213" mass="23908" checksum="5DBCC428066B6B37" modified="2019-05-08" version="1">MGTAEEDYNFVFKVVLIGESGVGKTNLLSRFTRNEFNHDSRTTIGVEFSTRTIMVGNAMVKAQIWDTAGLERYRAITSAYYRGAVGALLVFDITKHQTYDVVERWLKELYDHAEATIIVMLVGNKTDLAQAREVPTEEAKMFAENNGLLFIETSALDSTNVEQAFETILRDIFNKVQKQQQRSPQDNTVSLSSENPHGASQTPEEKRACCVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IL50</accession>
    <name>A0A452IL50_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RanBP-type and C3HC4-type zinc finger-containing protein 1</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">RING-type E3 ubiquitin transferase HOIL-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Ubiquitin-conjugating enzyme 7-interacting protein 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBR family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452IL50"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028631"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032522.1">
        <property type="protein sequence ID" value="ENSGAGP00000028631.1"/>
        <property type="gene ID" value="ENSGAGG00000020747.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071797">
        <property type="term" value="C:LUBAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097039">
        <property type="term" value="P:protein linear polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20345">
        <property type="entry name" value="BRcat_RBR_HOIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16633">
        <property type="entry name" value="mRING-HC-C3HC3D_RBR_HOIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20358">
        <property type="entry name" value="Rcat_RBR_HOIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01799">
        <property type="entry name" value="Ubl_HOIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000007">
        <property type="entry name" value="RanBP-type and C3HC4-type zinc finger-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000140">
        <property type="entry name" value="RanBP-type and C3HC4-type zinc finger-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000137">
        <property type="entry name" value="RanBP-type and C3HC4-type zinc finger-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000012">
        <property type="entry name" value="ranBP-type and C3HC4-type zinc finger-containing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047558">
        <property type="entry name" value="BRcat_RBR_HOIL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057468">
        <property type="entry name" value="HOIL-1/Sharpin_LTM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047559">
        <property type="entry name" value="HOIL1_RBR_mRING-HC-C3HC3D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047557">
        <property type="entry name" value="Rcat_RBR_HOIL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF35">
        <property type="entry name" value="RANBP-TYPE AND C3HC4-TYPE ZINC FINGER-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25393">
        <property type="entry name" value="LTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="21">
        <location>
            <begin position="439"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="443"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="671" mass="74299" checksum="D7D18E2E2D38BA32" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILF5</accession>
    <name>A0A452ILF5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ferritin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the ferritin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ILF5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028664"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032557.1">
        <property type="protein sequence ID" value="ENSGAGP00000028664.1"/>
        <property type="gene ID" value="ENSGAGG00000020773.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01056">
        <property type="entry name" value="Euk_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000002">
        <property type="entry name" value="Ferritin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014034">
        <property type="entry name" value="Ferritin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF54">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00204">
        <property type="entry name" value="FERRITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="2 9" id="KW-0409">Iron storage</keyword>
    <keyword evidence="3 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="10">
        <location>
            <begin position="8"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601519-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="177" mass="20321" checksum="0CA5E923DE8352AF" modified="2019-05-08" version="1">PAPSQVRQNFHALCEAAVNHLVNLELYASYVYLSMSYYFERDDVALRHVAQFLKEQSHEQKEHAEKFLTYQNKRGGSIVLQDIKKPEQDQWGNSLEALQCALKLEKTLNQALLDLQKLATEKNDPHLCDFLESDYLEKQVKAIKRLGDHVTNLKRLGVPQNGMGEYLFDKLTLGESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IM48</accession>
    <name>A0A452IM48_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Metabotropic glutamate receptor 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Dimeric G protein-coupled receptor which is activated by the excitatory neurotransmitter L-glutamate. Plays critical roles in modulating synaptic transmission and neuronal excitability. Upon activation by glutamate, inhibits presynaptic calcium channels, reducing further glutamate release and dampening excitatory signaling. Mechanistically, ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IM48"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028943"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032877.1">
        <property type="protein sequence ID" value="ENSGAGP00000028943.1"/>
        <property type="gene ID" value="ENSGAGG00000020966.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008066">
        <property type="term" value="F:glutamate receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007216">
        <property type="term" value="P:G protein-coupled glutamate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15447">
        <property type="entry name" value="7tmC_mGluR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06375">
        <property type="entry name" value="PBP1_mGluR_groupII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:001113">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:001127">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000001">
        <property type="entry name" value="metabotropic glutamate receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000029">
        <property type="entry name" value="Metabotropic glutamate receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001458">
        <property type="entry name" value="GPCR_3_mGluR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000162">
        <property type="entry name" value="GPCR_3_mtglu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050726">
        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24060">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01052">
        <property type="entry name" value="MTABOTROPC2R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00593">
        <property type="entry name" value="MTABOTROPICR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00979">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00980">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="591"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="630"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="662"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="704"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="753"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="784"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="812"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="20">
        <location>
            <begin position="592"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00057394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="895" mass="100200" checksum="85EC193A6FBEB982" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMD1</accession>
    <name>A0A452IMD1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IMD1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029079"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033033.1">
        <property type="protein sequence ID" value="ENSGAGP00000029079.1"/>
        <property type="gene ID" value="ENSGAGG00000021040.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000012">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="8">
        <location>
            <begin position="117"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="434" mass="49203" checksum="FEA6C9046DE7D9F3" modified="2019-05-08" version="1">MGRATGRKGRLGSYCLPKRKFYPPSMLSHIPSGQGAESAVPCAGIRYSELIGWNTKHGEEMLKKITVDPECFMNVTEIIRYHGYPSEEYEVITEDGYILSVNRIPGGKHSENTGQKPVVFLQHPLLGDATHWISNLDNNSLGFILADAGFDVWMGNGRGNTWSTKHKTLKPDQQKFWEFSFDEMGKYDITAVLYFAMNKTGQKQLYYVAHSEGTTLGFVAFSSRPDLAQRIKMFFALGPVATITFAKSPVIKLGRLPETLLKTVFGTKGLFHQNKHMKKPLILLCTTLNKFCACFLSSLAGRNEQNLNMSRLAMYVGHSPAGTSVQNILHWRQVLYSKQLQAYDYGSKEKNTEKYNQTTPPIYKIEELKMPIAVWSGGHDLFADPKDVAALLGRITNLVYHKHFPEWQHLDFIWGLDAAKRMYVKIIELMKKYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IMU0</accession>
    <name>A0A452IMU0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TBC1 domain family member 13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a GTPase-activating protein for RAB35. Together with RAB35 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with RAB1A and RAB10; in a GTP-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IMU0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029182"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033144.1">
        <property type="protein sequence ID" value="ENSGAGP00000029182.1"/>
        <property type="gene ID" value="ENSGAGG00000021111.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000009">
        <property type="entry name" value="TBC1 domain family member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000019">
        <property type="entry name" value="TBC1 domain family member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF27">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="9">
        <location>
            <begin position="35"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="400" mass="46510" checksum="B1EF92DE5B1B913F" modified="2019-05-08" version="1">MSSLHKSRIAEFQDVLGDPKIVLQKLRELCFSGIPFDGGLRCLCWKILLNYLPVEKALWSTLLKKQRELYSQFLKEMIIQPGIAKANLGVSREDVTLEDHPLNPNPDSRWNTYFKDNEVLLQIDKDVRRLYPDMAFFQRPTDYPCLLILDPQNEFETLRKRVEQTTLKSQTVARNRSGVTNVSSPLKTSAPNSLSEYEVLPNGCEAHWEVVERILFIYAKLNPGIAYVQGMNEIVGPLYYTFATDPNGDWKEHAEADTFFCFTNLMAEIRDNFIKSLDDSQCGITYKMEKVYSTLKEKDVELYLKLQEQNIKPQFFAFRWLTLLLSQEFLLPDVIRIWDSLFADDNRFDFLLLVCCAMLTLIRDQLLEGDFTLNMRLLQDYPISDVHLILKKAKELQDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPI2</accession>
    <name>A0A452IPI2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Direct IAP-binding protein with low pI</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Smac/DIABLO protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IPI2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029809"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033848.1">
        <property type="protein sequence ID" value="ENSGAGP00000029809.1"/>
        <property type="gene ID" value="ENSGAGG00000021528.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008631">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051402">
        <property type="term" value="P:neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000012">
        <property type="entry name" value="diablo homolog, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009062">
        <property type="entry name" value="Smac/DIABLO-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015142">
        <property type="entry name" value="Smac_DIABLO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32247">
        <property type="entry name" value="DIABLO HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32247:SF3">
        <property type="entry name" value="DIABLO IAP-BINDING MITOCHONDRIAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09057">
        <property type="entry name" value="Smac_DIABLO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46984">
        <property type="entry name" value="Smac/diablo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="216"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="242" mass="27069" checksum="B6C21B1827BF9113" modified="2019-05-08" version="1">MAAAVRSRWFRCCSALLRQGFPVLANARRRCFSGLSGQWNKMVGVGFGVALCAVPIVQKHEPTSLSHEALIRRAISLVTDSTCTLLSQTTYALIEALTEYTKAVYTLVSLYRQYTNHLGKMNSKEEDAVWQVIIGARVEMTTKQQEFLKLESSWMTALHLSEMAAEAAYQSGADQASVTARNHIQLVKTQVQEVRQLSQKAETKLAEAQTEELINTKVEESSQPCSISETIEEVEESYLRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IPL5</accession>
    <name>A0A452IPL5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000029880"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033932.1">
        <property type="protein sequence ID" value="ENSGAGP00000029880.1"/>
        <property type="gene ID" value="ENSGAGG00000021570.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000033997.1">
        <property type="protein sequence ID" value="ENSGAGP00000029938.1"/>
        <property type="gene ID" value="ENSGAGG00000021570.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072673">
        <property type="term" value="P:lamellipodium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01385">
        <property type="entry name" value="MYSc_Myo9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17217">
        <property type="entry name" value="RA_Myosin-IXb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04407">
        <property type="entry name" value="RhoGAP_myosin_IXB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000003">
        <property type="entry name" value="Putative unconventional myosin-IXa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000008">
        <property type="entry name" value="Putative unconventional myosin-IXa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000003">
        <property type="entry name" value="unconventional myosin-IXa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000009">
        <property type="entry name" value="unconventional myosin-IXa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000005">
        <property type="entry name" value="unconventional myosin-IXa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000013">
        <property type="entry name" value="unconventional myosin-IXa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000013">
        <property type="entry name" value="unconventional myosin-IXa isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000009">
        <property type="entry name" value="unconventional myosin-IXb isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046987">
        <property type="entry name" value="Myo9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036023">
        <property type="entry name" value="MYSc_Myo9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046989">
        <property type="entry name" value="RA_Myosin-IXb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028557">
        <property type="entry name" value="RhoGAP_myosin_IXB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46184:SF2">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IXB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46184">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IXB-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 16" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 16" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13 16" id="KW-0518">Myosin</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Ras-associating" evidence="20">
        <location>
            <begin position="15"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="22">
        <location>
            <begin position="145"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="19">
        <location>
            <begin position="1683"/>
            <end position="1732"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="21">
        <location>
            <begin position="1751"/>
            <end position="1939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="16">
        <location>
            <begin position="860"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1125"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1220"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1276"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1504"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1656"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1983"/>
            <end position="2013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="2034"/>
            <end position="2068"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="1066"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1148"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1220"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1276"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1303"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1342"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1380"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1517"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="1664"/>
            <end position="1674"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="238"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2217" mass="254795" checksum="0184903DA4C23850" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQI1</accession>
    <name>A0A452IQI1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Par3/HAL N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IQI1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000030099"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034172.1">
        <property type="protein sequence ID" value="ENSGAGP00000030099.1"/>
        <property type="gene ID" value="ENSGAGG00000021712.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051660">
        <property type="term" value="P:establishment of centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052213">
        <property type="entry name" value="PAR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021922">
        <property type="entry name" value="Par3/HAL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16484:SF4">
        <property type="entry name" value="PARTITIONING DEFECTIVE 3 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16484">
        <property type="entry name" value="PARTITIONING DEFECTIVE 3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12053">
        <property type="entry name" value="Par3_HAL_N_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Par3/HAL N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="79" mass="9172" checksum="93C3E6BEE03C7909" modified="2019-05-08" version="1">MKVTVSFGKTGIVVPCKDGRLSVRDLTQQALQRYLKTKEKVRQTHHITTSTSYQDHLRSLHLYTRQSAYRGASIYLQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IRM1</accession>
    <name>A0A452IRM1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Apolipoprotein B100 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000030507"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034615.1">
        <property type="protein sequence ID" value="ENSGAGP00000030507.1"/>
        <property type="gene ID" value="ENSGAGG00000021960.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034362">
        <property type="term" value="C:low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034359">
        <property type="term" value="C:mature chylomicron"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050750">
        <property type="term" value="F:low-density lipoprotein particle receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030301">
        <property type="term" value="P:cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006642">
        <property type="term" value="P:triglyceride mobilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022176">
        <property type="entry name" value="ApoB100_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052418">
        <property type="entry name" value="Apolipoprotein_B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13769">
        <property type="entry name" value="APOLIPOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13769:SF1">
        <property type="entry name" value="APOLIPOPROTEIN B-100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12491">
        <property type="entry name" value="ApoB100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="6" id="KW-0162">Chylomicron</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="11" id="KW-0427">LDL</keyword>
    <keyword evidence="10" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="15" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="14" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="16" id="KW-0850">VLDL</keyword>
    <feature type="domain" description="Apolipoprotein B100 C-terminal" evidence="18">
        <location>
            <begin position="3298"/>
            <end position="3353"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="1081"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="3069"/>
            <end position="3119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="3371" mass="382290" checksum="AC990E195DEA962E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ISG1</accession>
    <name>A0A452ISG1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Codanin-1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000030964"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035109.1">
        <property type="protein sequence ID" value="ENSGAGP00000030964.1"/>
        <property type="gene ID" value="ENSGAGG00000022251.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040031">
        <property type="entry name" value="Codanin-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028171">
        <property type="entry name" value="Codanin-1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28678">
        <property type="entry name" value="CODANIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28678:SF1">
        <property type="entry name" value="CODANIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15296">
        <property type="entry name" value="Codanin-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Codanin-1 C-terminal" evidence="2">
        <location>
            <begin position="879"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1328" mass="148053" checksum="D4B066833878086E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IST7</accession>
    <name>A0A452IST7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="15">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Homodimerization via the leucine zipper domains is required for autophosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. Interacts with TLR4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A452IST7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031088"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035253.1">
        <property type="protein sequence ID" value="ENSGAGP00000031088.1"/>
        <property type="gene ID" value="ENSGAGG00000022339.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004706">
        <property type="term" value="F:JUN kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12058">
        <property type="entry name" value="SH3_MLK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14146">
        <property type="entry name" value="STKc_MLK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000076">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000169">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000085">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016231">
        <property type="entry name" value="MLK1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF30">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000556">
        <property type="entry name" value="MAPKKK9_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 18" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SH3" evidence="21">
        <location>
            <begin position="21"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="126"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="525"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="678"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="745"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="683"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="745"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="132"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 19">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00065138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000556-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000556-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1037" mass="115587" checksum="09323F10AA7498D7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IT64</accession>
    <name>A0A452IT64_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IT64"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031231"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035425.1">
        <property type="protein sequence ID" value="ENSGAGP00000031231.1"/>
        <property type="gene ID" value="ENSGAGG00000022451.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004741">
        <property type="term" value="F:[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF236">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="102"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="464" mass="52293" checksum="382E0BBD2AC5EE62" modified="2019-05-08" version="1">MSSVWFKKHFLKSGSSTKPGNAESLGRGAEQQLPAASLYRRPKFLRGVCSGERLPGRRPIYCPPRDRPLQWGTGYAEVINAEKSEFNEDQATCCQISVRRREIGMEEEQDWLILCSSQYLIGYYWALFDGHGGPDAAIIASNYLHYCIKQKLEEVVEGITEAQPPMHLSGCCICPSDSQFVEEKQIQQEDVVIGALENAFQECDEVIGQEMEATNQSGGCTALAALYLQGKLYVANAGDSRAILVQKQSVVPLSSEFTPETERQRIQQLAFLCPKLLADEFTRFEFPRRLKGNDVGQKILYRDYSMAGWGYKTVEKADLKYPLIHGHGKQTRLLGTLAVSRGLGDHQLKVIDTDIEVKPFLSCIPQVKVFDFASRNIKEDDVLVLATDGLWDILSNEDVAQIVRSFLKENRTDPYRFSELARCLVHTARGKKNGHQWILDDNSPASYDDISVFLIPLYNRDEDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITK7</accession>
    <name>A0A452ITK7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ITK7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031289"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035490.1">
        <property type="protein sequence ID" value="ENSGAGP00000031289.1"/>
        <property type="gene ID" value="ENSGAGG00000022486.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031685">
        <property type="entry name" value="TEX29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37339">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37339:SF1">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15839">
        <property type="entry name" value="TEX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="141" mass="15580" checksum="3977391FF9441D63" modified="2019-05-08" version="1">MKNATFNGITLIANVKSVALFFVFFFVVCELPLYEICSQNVSRVQCSALGCCFHKQICYRKAVPCKYLESNMINSRSKTSFVILKTETQSSTRPARTTVTKPADVSPVLQIATPLITHLSGSMGPTQHVVVCIMDQTTQIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IV38</accession>
    <name>A0A452IV38_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IV38"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031879"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036157.1">
        <property type="protein sequence ID" value="ENSGAGP00000031879.1"/>
        <property type="gene ID" value="ENSGAGG00000022889.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005374">
        <property type="entry name" value="BBLN_eukaryota"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34344:SF1">
        <property type="entry name" value="BUBLIN COILED-COIL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34344">
        <property type="entry name" value="UPF0184 PROTEIN C9ORF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03670">
        <property type="entry name" value="UPF0184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="94" mass="10544" checksum="FB36FC0BCDA3F280" modified="2019-05-08" version="1">MSGPNGEPHVPAGNVGRSEEGDDDGFGETEYAAINSMLDQINSCLDHLEEKNDHLHARLKELLESNRQTRLEFQQQLSEEQNMQADVQGPHMDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWD5</accession>
    <name>A0A452IWD5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000032349"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036674.1">
        <property type="protein sequence ID" value="ENSGAGP00000032349.1"/>
        <property type="gene ID" value="ENSGAGG00000023152.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000036675.1">
        <property type="protein sequence ID" value="ENSGAGP00000032350.1"/>
        <property type="gene ID" value="ENSGAGG00000023152.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05529">
        <property type="entry name" value="Bromo_WDR9_I_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05496">
        <property type="entry name" value="Bromo_WDR9_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000017">
        <property type="entry name" value="Bromodomain and WD repeat domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000023">
        <property type="entry name" value="Bromodomain and WD repeat domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000071">
        <property type="entry name" value="Bromodomain and WD repeat domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000003">
        <property type="entry name" value="Bromodomain and WD repeat domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000066">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052060">
        <property type="entry name" value="Bromo_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057451">
        <property type="entry name" value="BRWD/PHIP_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057452">
        <property type="entry name" value="BRWD/PHIP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16266:SF26">
        <property type="entry name" value="BROMODOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16266">
        <property type="entry name" value="WD REPEAT DOMAIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25437">
        <property type="entry name" value="BRWD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25313">
        <property type="entry name" value="BRWD_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="185"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="227"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="269"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="367"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="432"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="467"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="7">
        <location>
            <begin position="1181"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="7">
        <location>
            <begin position="1338"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="668"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="781"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="894"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1448"/>
            <end position="1615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1704"/>
            <end position="1955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2000"/>
            <end position="2019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2056"/>
            <end position="2271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2366"/>
            <end position="2663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="796"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="834"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="857"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="908"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="927"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1453"/>
            <end position="1463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1504"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1541"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1712"/>
            <end position="1727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1758"/>
            <end position="1771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1806"/>
            <end position="1817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1878"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2067"/>
            <end position="2077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2118"/>
            <end position="2129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2131"/>
            <end position="2148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2181"/>
            <end position="2204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2212"/>
            <end position="2231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2234"/>
            <end position="2246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="2247"/>
            <end position="2258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2366"/>
            <end position="2376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2379"/>
            <end position="2388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2394"/>
            <end position="2409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="2416"/>
            <end position="2426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2443"/>
            <end position="2459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2472"/>
            <end position="2482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2549"/>
            <end position="2567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="2570"/>
            <end position="2588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="2597"/>
            <end position="2608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="2623"/>
            <end position="2641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2642"/>
            <end position="2657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2663" mass="300061" checksum="4DB64BB07FE1A3C4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IWU9</accession>
    <name>A0A452IWU9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IWU9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032566"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036915.1">
        <property type="protein sequence ID" value="ENSGAGP00000032566.1"/>
        <property type="gene ID" value="ENSGAGG00000023293.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902236">
        <property type="term" value="P:negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904292">
        <property type="term" value="P:regulation of ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17118">
        <property type="entry name" value="Ubl_HERP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000046">
        <property type="entry name" value="Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039751">
        <property type="entry name" value="HERPUD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12943:SF7">
        <property type="entry name" value="HOMOCYSTEINE-RESPONSIVE ENDOPLASMIC RETICULUM-RESIDENT UBIQUITIN-LIKE DOMAIN MEMBER 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12943">
        <property type="entry name" value="HOMOCYSTEINE-RESPONSIVE ENDOPLASMIC RETICULUM-RESIDENT UNIQUITIN-LIKE DOMAIN HERPUD PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0834">Unfolded protein response</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="4"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="382" mass="43230" checksum="09C774C0FA02EF52" modified="2019-05-08" version="1">MEPFDLLVRSPTQRHPDLRLRGEPGWTVRQLKARLRRLHPEEPPEEDQKLIYSGKLLLDHLYLRDVLPKQEKFHALHLVYHSKTLSKMKETNAKIDETNQQPKTVSGPGQDPCTVSSNDGLRQRETSCNQLSTGEANTRIDTAQHPHGVAPGYSAYTTYNILQMSWFQQMYARQYYMQYLAAVSADRSPIQRSQEIPVAPVAAPAPLPDAFPAQNLPGNHNAPPQLNAGANQNLQMNAQGGPLMEEEEEMNRDWLDWLYSATLFLVFVNIIYFYSSLSRFLMVMGGTVLMYLHHAGWFPFRRQPVHPLPNNALPQAVINQDQNNNLQEENVGGEAEPEAVSEEGRALPENQQDSPSFMNTAWLFFKTFFASLLPEGPPAVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IX41</accession>
    <name>A0A452IX41_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Glutathione hydrolase 5 proenzyme</fullName>
            <ecNumber evidence="4">2.3.2.2</ecNumber>
            <ecNumber evidence="5">3.4.19.13</ecNumber>
            <ecNumber evidence="25">3.4.19.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">Gamma-glutamyl leukotrienase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Gamma-glutamyltransferase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">Gamma-glutamyltransferase-like activity 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Gamma-glutamyltranspeptidase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Leukotriene-C4 hydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>S-[(2E,6E,10E)-geranylgeranyl]-L-glutathione + H2O = S-[(2E,6E,10E)-geranylgeranyl]-L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:65120"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:156326"/>
            <dbReference type="ChEBI" id="CHEBI:156330"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:65121"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>an N-terminal (5-L-glutamyl)-[peptide] + an alpha-amino acid = 5-L-glutamyl amino acid + an N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:23905"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:59469"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:28807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:28808"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>leukotriene C4 + H2O = leukotriene D4 + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:31563"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:63166"/>
            <dbReference type="EC" id="3.4.19.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:31564"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="24">Lipid metabolism; leukotriene D4 biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sulfur metabolism; glutathione metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Heterodimer composed of the light and heavy chains. The active site is located in the light chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.2" evidence="4"/>
    <dbReference type="EC" id="3.4.19.13" evidence="5"/>
    <dbReference type="EC" id="3.4.19.14" evidence="25"/>
    <dbReference type="AlphaFoldDB" id="A0A452IX41"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032635"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036988.1">
        <property type="protein sequence ID" value="ENSGAGP00000032635.1"/>
        <property type="gene ID" value="ENSGAGG00000023330.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002951">
        <property type="term" value="F:leukotriene-C(4) hydrolase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019370">
        <property type="term" value="P:leukotriene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.130:FF:000001">
        <property type="entry name" value="Gamma-glutamyltransferase 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.40:FF:000005">
        <property type="entry name" value="gamma-glutamyltransferase 5 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055262">
        <property type="entry name" value="GGT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45027:SF2">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45027">
        <property type="entry name" value="PUTATIVE GLUTATHIONE HYDROLASE LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00462">
        <property type="entry name" value="G_GLU_TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0434">Leukotriene biosynthesis</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="32">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <begin position="320"/>
            <end position="322"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00033643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00050507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00052640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00066923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00071554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00075099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00075858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00079251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00081975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00082872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="479" mass="52961" checksum="9F17102459E004ED" modified="2019-05-08" version="1">MGLGGGVIFTIYNATTGEVVVINARETAPENINQDLLNQCNDFALQIGSQWIAVPGELRGYEEAHRRYGRLPWKWLFEPTIQLLTHGIRMPAALSKFLSHPLLQSFLKKSSLCQLFCENGVVLKPDAIIFWPTLLKTLKAVAEKGAREFYEGEIAQNLVKDIKHENGNLMLSDLKNFQAKVMTPLNISLGGYTMYSPPPPAGGAVLLFILNILKEFKFTPESLEPANRKIETYHRIAEALKFGNGQKPKLDDPQFSGIQQVIIQELLSNSFAELVRKRIDDRGDHPGSFYNLSQPSNTGYGTSHVSVLAEDGSAVSVTSTINQPFGSMVYSPSTGIILNNELADFCKKKPKNKITSGERPPSDMVPSILVSKDDNSKLVIGGSGGDLIISATALAIMNKLWFGYDLERAISLPVLHASMNNSIQFEPGFDEAVKGELLRRGHHEQKRTMFLNVVQGISKEGRCIFAYSDRRKLGKSSGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IX42</accession>
    <name>A0A452IX42_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IX42"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032498"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036845.1">
        <property type="protein sequence ID" value="ENSGAGP00000032498.1"/>
        <property type="gene ID" value="ENSGAGG00000023248.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28159" checksum="1F853EAE6109758C" modified="2019-05-08" version="1">MAPSRAGYATLKTVMICFNVVGCTMVGLGLWIKLGSASFVRVLGTTSVYFAHVGYFCIVVGSVLVVLGFMGCWGAVKENRCLLLTVSAHSRCRLGRKKIFSGSRPISFPPFKARSIVLDKSLTALKKKYSGYKHDDIVSYGWNAFMLKVGDPLPPRPPVSKDLTHLFPRITVAPRGLTQCARCCTYTVTAPAVTRWCERKDSLVVRTENPGSVPVSPLHSLCDLGPVTGAPWAPVPHWYHGDHSREVGSRCPAGTYMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IY55</accession>
    <name>A0A452IY55_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IY55"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032987"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037390.1">
        <property type="protein sequence ID" value="ENSGAGP00000032987.1"/>
        <property type="gene ID" value="ENSGAGG00000023555.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="145" mass="14815" checksum="31D677531A9C676B" modified="2019-05-08" version="1">MWFSPACGQPGSVSITWFSPACGQPGSVSITWFSPACGQPGSVSITWFSPACGQPGSVSITWFSPACGQPGSVSITWFSPACGQPGSVSITWFSPACGQPGSVSITWFSPACGQPGSVSITCGEELRPRRSHAACCSGTLLEAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IZC1</accession>
    <name>A0A452IZC1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the apolipoprotein L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IZC1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033403"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037844.1">
        <property type="protein sequence ID" value="ENSGAGP00000033403.1"/>
        <property type="gene ID" value="ENSGAGG00000023800.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045601">
        <property type="term" value="P:regulation of endothelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008405">
        <property type="entry name" value="ApoL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096">
        <property type="entry name" value="APOLIPOPROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096:SF1">
        <property type="entry name" value="APOLIPOPROTEIN L DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05461">
        <property type="entry name" value="ApoL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="246" mass="26823" checksum="3B65DF6F5776957E" modified="2019-05-08" version="1">MERDAITFPPALDPTRHFQMLLLNQRSKLHGQIRKLREIARNINKLRRRSLIANITGSSLSAVGAITAIVGLSLSPATLGASLLASAVGLGVASAGGAVNVTSDLSLVLSNSRELRRVQEIAVNCQNQMREILSCLEFLHRGQGPMDPLLLQSEKNASISLYNSICFMVFYGSRNFLVPEYTREVTKVSQAVLKAKIQKLAENLESCIRAMDEVCELLESRAELSSNTRNPSSSARITIKPQGSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J2H9</accession>
    <name>A0A452J2H9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Motile sperm domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in differentiation and/or proliferation of mesenchymal stem cells. Proposed to be involved in epithelial-to-mesenchymal transition (EMT). However, another study suggests that it is not required for EMT or stem cell self-renewal and acts during later stages of differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000034609"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039184.1">
        <property type="protein sequence ID" value="ENSGAGP00000034609.1"/>
        <property type="gene ID" value="ENSGAGG00000024600.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000039186.1">
        <property type="protein sequence ID" value="ENSGAGP00000034611.1"/>
        <property type="gene ID" value="ENSGAGG00000024600.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000431">
        <property type="entry name" value="motile sperm domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039283">
        <property type="entry name" value="MOSPD1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34441">
        <property type="entry name" value="MOTILE SPERM DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34441:SF2">
        <property type="entry name" value="MOTILE SPERM DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="11">
        <location>
            <begin position="17"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="215" mass="24305" checksum="927C0775443F4FCC" modified="2019-05-08" version="1">MQQQKRQPELVEGNLPVFVFPTELIFYADDQSTHKQVLTLYNPYEFALKFKVLCTTPNKYVVVDAAGAVKPQCCVDIVXRHRDVRSCHYGMIDKFRLQVSEQSQRKALGRKEVIATLLPSAKEQQQKEEEEKRIKEHLAESVFFEQTLCQPENRSASSGPSLLTVFLGIVCIGALMLPTLGEVESLVPLYLHLSVNQKLVAAYVLGLITMVILRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J3N6</accession>
    <name>A0A452J3N6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bcl-2-related protein A1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection. Can inhibit apoptosis induced by serum starvation in the mammary epithelial cell line HC11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Bcl-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J3N6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035029"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039664.1">
        <property type="protein sequence ID" value="ENSGAGP00000035029.1"/>
        <property type="gene ID" value="ENSGAGG00000024904.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06845">
        <property type="entry name" value="Bcl-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.437.10:FF:000008">
        <property type="entry name" value="Bcl-2-related protein A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020717">
        <property type="entry name" value="Bcl2_BH1_motif_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020726">
        <property type="entry name" value="Bcl2_BH2_motif_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013282">
        <property type="entry name" value="Bcl2A1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF10">
        <property type="entry name" value="BCL-2-RELATED PROTEIN A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01862">
        <property type="entry name" value="BCL2FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01867">
        <property type="entry name" value="BCL2RLATEDA1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00337">
        <property type="entry name" value="BCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01080">
        <property type="entry name" value="BH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01258">
        <property type="entry name" value="BH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="7">
        <location>
            <begin position="37"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="175" mass="20508" checksum="301D256C02927362" modified="2019-05-08" version="1">MESSEYCYVYYLVQDYLKYVLQEPQLGPAPSRVAHVLRHAASFLQKENEESLKPCLDTFDITSVDAARRIFTQVMDKEFADGNTNWGRILTIFMFGGILSKKLQEHRVQLTGENKKQISYFITEYIINTKAEWIEANGGWENGFLTMFEEKRSWLSLFNIKAKIMDAFSFFSQYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J435</accession>
    <name>A0A452J435_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Protein phosphatase 1B</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Protein phosphatase 2C isoform beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="21">Monomer. Interacts with PAK6. Interacts with the phosphorylated form of IKBKB/IKKB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 24">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452J435"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035192"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039848.1">
        <property type="protein sequence ID" value="ENSGAGP00000035192.1"/>
        <property type="gene ID" value="ENSGAGG00000025014.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.430:FF:000001">
        <property type="entry name" value="protein phosphatase 1B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000001">
        <property type="entry name" value="protein phosphatase 1B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.430">
        <property type="entry name" value="Phosphatase 2C, C-terminal domain suprefamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012911">
        <property type="entry name" value="PP2C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036580">
        <property type="entry name" value="PP2C_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07830">
        <property type="entry name" value="PP2C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81601">
        <property type="entry name" value="Protein serine/threonine phosphatase 2C, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 24" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="18" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 24" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="26">
        <location>
            <begin position="23"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="429"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00065584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00070216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00082952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="479" mass="53131" checksum="23934DCBCD8FC1F0" modified="2019-05-08" version="1">MGAFLDKPKTDKHNAHGTGNGLRYGLSSMQGWRVEMEDAHTAVVGIPHGLEDWSFFAVYDGHAGSRVANYCSTHLLEHITNNDDFRASEKPGSALEPSVENVKSGIRTGFLKIDEYMRNFSDLRNGMDRSGSTAVGVMISREHIYFINCGDSRAVLYRNGQVCFSTQDHKPCNPREKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCVDGKGPTEQLVSPEPEVYEILRAEEDEFIILACDGIWDVMSNEELCEFVKSRLEVSDDLEKVCNWVVDTCLHKGSRDNMSIVLVCFSNAPKVSDEAVKKDTELDKHLESRVEEIMEKSGEEGMPDLAQVMRILSAENIPNLPPGGGLAGKRNIIEAVYNRLNPHRENDGDPGEPEESGTQGKLVEALRQMRINHRGNYRHLLEEMLTSYRLAKIQGEECTAEPATASTSDNLAGPSDPVADTQTESENHLAEMQSSNEDSGRNEMSDKQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J4W7</accession>
    <name>A0A452J4W7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">cAMP-dependent protein kinase type I-alpha regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J4W7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035498"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040202.1">
        <property type="protein sequence ID" value="ENSGAGP00000035498.1"/>
        <property type="gene ID" value="ENSGAGG00000025236.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd12101">
        <property type="entry name" value="DD_RIalpha_PKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000013">
        <property type="entry name" value="cAMP-dependent protein kinase type I regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.890.10:FF:000001">
        <property type="entry name" value="cAMP-dependent protein kinase type I-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000006">
        <property type="entry name" value="cAMP-dependent protein kinase type I-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF129">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I-ALPHA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10 13" id="KW-0114">cAMP</keyword>
    <keyword evidence="5 13" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="138"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="256"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="64"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="382" mass="43337" checksum="37C2D6DA9544CDCC" modified="2019-05-08" version="1">MAASSSNTSEEEHSLRECELYVQKHNIQQLLKDCIVQLCTVRPERPMGFLREYFERLEKEEAKQLLNQQKSGSRSDSREDEISPPPPMNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFAHLDDNERSDIFDAMFPVTYIAGETVIQQGDEGDNFYVVDQGEMDVYVNNEWATNVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGTAAVLQRRSENEEFVEVGRLAPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J574</accession>
    <name>A0A452J574_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leucine-rich repeat and death domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Leucine-rich repeat protein SHOC-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein soc-2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4 5">protein Sur-8 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452J574"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035628"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040344.1">
        <property type="protein sequence ID" value="ENSGAGP00000035628.1"/>
        <property type="gene ID" value="ENSGAGG00000025302.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000307">
        <property type="entry name" value="Leucine-rich repeats and death domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056869">
        <property type="entry name" value="DD_LRRD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF54">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24978">
        <property type="entry name" value="Death_Lrrd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="8">
        <location>
            <begin position="562"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="LRRD1 death" evidence="9">
        <location>
            <begin position="762"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="843" mass="95754" checksum="632EDF14E84938F7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5J7</accession>
    <name>A0A452J5J7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Lanosterol 14-alpha demethylase</fullName>
            <ecNumber evidence="22">1.14.14.154</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="39 40">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="40" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="39" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="39" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>24,25-dihydrolanosterol + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:45960"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:28113"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:78904"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:45961"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>24,25-dihydrolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxy-24,25-dihydrolanosterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75079"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:28113"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:87057"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:75080"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>32-hydroxy-24,25-dihydrolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxo-24,25-dihydrolanosterol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75087"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:87057"/>
            <dbReference type="ChEBI" id="CHEBI:87060"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:75088"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>32-hydroxylanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxolanosterol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75107"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166681"/>
            <dbReference type="ChEBI" id="CHEBI:166806"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:75108"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>32-oxo-24,25-dihydrolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:75083"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:78904"/>
            <dbReference type="ChEBI" id="CHEBI:87060"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:75084"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>32-oxolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:75111"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166681"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:75112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>a 14alpha-formyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a Delta(14) steroid + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:68068"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138031"/>
            <dbReference type="ChEBI" id="CHEBI:176902"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:68069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a 14alpha-hydroxymethyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-formyl steroid + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68064"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:176901"/>
            <dbReference type="ChEBI" id="CHEBI:176902"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:68065"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a 14alpha-methyl steroid + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = a Delta(14) steroid + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54028"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138029"/>
            <dbReference type="ChEBI" id="CHEBI:138031"/>
            <dbReference type="EC" id="1.14.14.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:54029"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>a 14alpha-methyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-hydroxymethyl steroid + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138029"/>
            <dbReference type="ChEBI" id="CHEBI:176901"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:68061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>lanosterol + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:25286"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:25287"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>lanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxylanosterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75103"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166806"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:75104"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 36">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="21">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 37">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.154" evidence="22"/>
    <dbReference type="AlphaFoldDB" id="A0A452J5J7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035631"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040347.1">
        <property type="protein sequence ID" value="ENSGAGP00000035631.1"/>
        <property type="gene ID" value="ENSGAGG00000025304.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008398">
        <property type="term" value="F:sterol 14-demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11042">
        <property type="entry name" value="CYP51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000027">
        <property type="entry name" value="lanosterol 14-alpha demethylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF2">
        <property type="entry name" value="24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="5" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 36 37" id="KW-0349">Heme</keyword>
    <keyword evidence="14 36 37" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="18 38" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 36 37" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 37" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="13 37" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="40" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="20" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="15" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="19" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="7 38" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 38" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="38">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="36">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00037887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00038974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00041158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00047702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00047983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00048736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00048839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00048866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00049163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00049450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="496" mass="56325" checksum="16E1C4CF17DA47EB" modified="2019-05-08" version="1">MPSLLDVGGFMLEWVATGSPLSMVLVAAAFVLSLGYLLQLGFRSQPRPGQGKYPPYISSNIPFLGHAVAFGKNPIEFLENAYEKYGPVFSFTMVGKTFTYLLGSDAAALIFNSKNEDLNAEDVYSRLTTPVFGKGVAYDVPNPVFLEQKKMLKTGLNVAQFKQHVPVIEKETKEYFKSWGDSGERNLFEALSELIILTASHCLHGKEIRSLLNEKVAQLYADLDGGFTHAAWLLPGWLPLPSFRRRDRAHREIKNIFYKVIQKRRQSGEREDDMVQTLLDVTYKDGRPLTDDEIAGMLIGLLLAGQHTSSTTSAWLGFFLARDKSIQEQCYAEQKAVCGEDLRPLNYDQLKELNLLDRCLKETLRLRPPIMTMMRMARTPQTVAGYTVPPGHQVCVSPTVNHRLKDSWIENLEFKPDRYLQDNPTAGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSILLRLYEFDLIDGYFPTINYTTMIHTPDNPVIRYKKRSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFE9</accession>
    <name>A0A452GFE9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Merlin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Moesin-ezrin-radixin-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Neurofibromin-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Schwannomin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GFE9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000291"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000331.1">
        <property type="protein sequence ID" value="ENSGAGP00000000291.1"/>
        <property type="gene ID" value="ENSGAGG00000000261.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13194">
        <property type="entry name" value="FERM_C_ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17186">
        <property type="entry name" value="FERM_F1_Merlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000103">
        <property type="entry name" value="Merlin isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000003">
        <property type="entry name" value="Radixin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000002">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.450:FF:000001">
        <property type="entry name" value="radixin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011174">
        <property type="entry name" value="ERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011259">
        <property type="entry name" value="ERM_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041789">
        <property type="entry name" value="ERM_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046810">
        <property type="entry name" value="ERM_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008954">
        <property type="entry name" value="Moesin_tail_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23281">
        <property type="entry name" value="MERLIN/MOESIN/EZRIN/RADIXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00769">
        <property type="entry name" value="ERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20492">
        <property type="entry name" value="ERM_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002305">
        <property type="entry name" value="ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48678">
        <property type="entry name" value="Moesin tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="FERM" evidence="19">
        <location>
            <begin position="22"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="446"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="518"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="76"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR002305-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="594" mass="69646" checksum="196E0D1107EFD8E8" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFL0</accession>
    <name>A0A452GFL0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">2-oxoisovalerate dehydrogenase subunit beta, mitochondrial</fullName>
            <ecNumber evidence="3">1.2.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Branched-chain alpha-keto acid dehydrogenase E1 component beta chain</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Together with BCKDHA forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 3-methyl-2-oxobutanoate + H(+) = N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13457"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10497"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83142"/>
            <dbReference type="EC" id="1.2.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:13458"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterotetramer of 2 alpha/BCKDHA and 2 beta chains/BCKDHB that forms the branched-chain alpha-keto acid decarboxylase (E1) component of the BCKD complex. The branched-chain alpha-ketoacid dehydrogenase is a large complex composed of three major building blocks E1, E2 and E3. It is organized around E2, a 24-meric cubic core composed of DBT, to which are associated 6 to 12 copies of E1, and approximately 6 copies of the dehydrogenase E3, a DLD dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452GFL0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000351"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000395.1">
        <property type="protein sequence ID" value="ENSGAGP00000000351.1"/>
        <property type="gene ID" value="ENSGAGG00000000301.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:branched-chain 2-oxo acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007584">
        <property type="term" value="P:response to nutrient"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000004">
        <property type="entry name" value="2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42980:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42980">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="12">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="322" mass="35441" checksum="E362F2A0276AA9A3" modified="2019-05-08" version="1">MNLFQSITSALDNTLSSDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQGIVGFGIGVAVAGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGCVGHGALYHSQSPEAFFAHCPGIKVVIPRSPFQAKGLLLSCIEDKNPCIFFEPKILYRAAVEQVPVEPYYIPLSQAEILQEGSDVTLVAWGTQVHVIREVATMAQEKLGVSCEVIDLKTILPWDAEAVCKSVVKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFPHIFEPFYIPDKWKCYDALRKMINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFW2</accession>
    <name>A0A452GFW2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ras homolog, mTORC1 binding</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GFW2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000429"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000482.1">
        <property type="protein sequence ID" value="ENSGAGP00000000429.1"/>
        <property type="gene ID" value="ENSGAGG00000000339.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019489394" description="Ras homolog, mTORC1 binding" evidence="3">
        <location>
            <begin position="25"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17212" checksum="CD766EBC087C4A07" modified="2019-05-08" version="1">GPKSKFHAFLLFPLAFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQFSKIIANDSKIASASSFKFFSGIDQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GG42</accession>
    <name>A0A452GG42_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GG42"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000514"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000573.1">
        <property type="protein sequence ID" value="ENSGAGP00000000514.1"/>
        <property type="gene ID" value="ENSGAGG00000000415.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001009">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001119">
        <property type="entry name" value="zinc finger protein 408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000180">
        <property type="entry name" value="Zinc finger protein 689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF377">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="257" mass="27950" checksum="CC746EFB8F37BE1D" modified="2019-05-08" version="1">GDTPRHTHPGSPGVCPAGEDRPCPAWGSGCASDDPGCGSNLCRHQNLHAVGEGPLCCPECGESFTGSAGLARHRRTHAVGERSHYCHQCGKGFASHSLLTIHRRSHSGERPFSCAQCERSFDRSSLLVEHQRTHTGERPYRCHQCSQGFTRATHLSRHQLIHCGERPHGCPECGQCFRLRAALVKHVRSHTGERPYKCAECGAVFASVSSLARHRRSHAGPCALTKALNPRAPWAGAPAFRSSVTKESQSSERTEPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHQ4</accession>
    <name>A0A452GHQ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dehydrogenase/reductase SDR family member 11</fullName>
            <ecNumber evidence="3">1.1.1.270</ecNumber>
            <ecNumber evidence="4">1.1.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">17-beta-hydroxysteroid dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">3-beta-hydroxysteroid 3-dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Estradiol 17-beta-dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Short-chain dehydrogenase/reductase family 24C member 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of the 17-keto group of estrone, 4- and 5-androstenes and 5-alpha-androstanes into their 17-beta-hydroxyl metabolites and the conversion of the 3-keto group of 3-, 3,17- and 3,20- diketosteroids into their 3-hydroxyl metabolites. Exhibits reductive 3-beta-hydroxysteroid dehydrogenase activity toward 5-beta-androstanes, 5-beta-pregnanes, 4-pregnenes and bile acids. May also reduce endogenous and exogenous alpha-dicarbonyl compounds and xenobiotic alicyclic ketones.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>17beta-estradiol + NAD(+) = estrone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16469"/>
            <dbReference type="ChEBI" id="CHEBI:17263"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.62"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>17beta-estradiol + NADP(+) = estrone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24616"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16469"/>
            <dbReference type="ChEBI" id="CHEBI:17263"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.62"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 3beta-hydroxysteroid + NADP(+) = a 3-oxosteroid + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:34787"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36836"/>
            <dbReference type="ChEBI" id="CHEBI:47788"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.270"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Steroid biosynthesis; estrogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 15">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.270" evidence="3"/>
    <dbReference type="EC" id="1.1.1.62" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452GHQ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001107"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001242.1">
        <property type="protein sequence ID" value="ENSGAGP00000001107.1"/>
        <property type="gene ID" value="ENSGAGG00000000899.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000253">
        <property type="term" value="F:3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004303">
        <property type="term" value="F:estradiol 17-beta-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05343">
        <property type="entry name" value="Mgc4172-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00077379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="225" mass="24784" checksum="B1D566C7AD4F64B1" modified="2019-05-08" version="1">MKVVGCARSVDKIEKLAAECQSAGYSGVLIPYKCDLSNEEEILSMFSAIKTLHQGVDVCINNAGLARPEPLLSGKTEGWRMMIDVNVMAVSICTREVYRSMKERNIDDGHIININSMNGHSVVPQSVVHFYSATKYAVTALTEGLRQELREAKTHIRATCISPGLVETGFAFKLHDNDPERAAATYESIRCLKAEDMANAVIYVLSAPPHVQIGDIQMRPTEQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GI49</accession>
    <name>A0A452GI49_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF1907 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GI49"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001312"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001471.1">
        <property type="protein sequence ID" value="ENSGAGP00000001312.1"/>
        <property type="gene ID" value="ENSGAGG00000001055.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000001480.1">
        <property type="protein sequence ID" value="ENSGAGP00000001320.1"/>
        <property type="gene ID" value="ENSGAGG00000001055.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17298">
        <property type="entry name" value="DUF1907"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015021">
        <property type="entry name" value="C11orf54_DUF1907"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13204:SF1">
        <property type="entry name" value="ESTER HYDROLASE C11ORF54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13204">
        <property type="entry name" value="PTD012 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08925">
        <property type="entry name" value="DUF1907"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01168">
        <property type="entry name" value="DUF1907"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DUF1907" evidence="7">
        <location>
            <begin position="20"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="316" mass="34776" checksum="FD04E9797D022E25" modified="2019-05-08" version="1">MAKVEKFALHVPSLEELSGVLRNGLKENFADVQVSVVECPDLTHDPFNFPVKGICGKPRIADVGGVPYLVPLVQKDKVYDLNTVAKQIELPGAFILGAGAGSSRILGVNAELIPVAQAESEQKPAVNASYTAQINPADGGCLLEKYSNKYSDCEFGLLANLYASQGQPGKVIEVKANGRTGRHNFVTCMRQIIEKCYGDKPVGIGGTFVIQKGKAKIHIMPPEFSACPLNTDEEMNNWLHFFEMKAPLICQPVLISRDPGFDLRMEHTHCFSHHGEGGHYHNDTTPDCVQYLGYFLPAEVLFRIDRPKESHRVGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GIB5</accession>
    <name>A0A452GIB5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">TIR domain-containing adapter molecule 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">TRIF-related adapter molecule</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Functions as a sorting adapter in different signaling pathways to facilitate downstream signaling leading to type I interferon induction. In TLR4 signaling, physically bridges TLR4 and TICAM1 and functionally transmits signal to TICAM1 in early endosomes after endocytosis of TLR4. In TLR2 signaling, physically bridges TLR2 and MYD88 and is required for the TLR2-dependent movement of MYD88 to endosomes following ligand engagement. Involved in IL-18 signaling and is proposed to function as a sorting adapter for MYD88 in IL-18 signaling during adaptive immune response. Forms a complex with RAB11FIP2 that is recruited to the phagosomes to promote the activation of the actin-regulatory GTPases RAC1 and CDC42 and subsequent phagocytosis of Gram-negative bacteria.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Homodimer. Interacts with TLR4, TICAM1, IRF3 and IRF7 in response to LPS. Interacts with IL1R1, IL1RAP, IRAK2, IRAK3 and TRAF6. Interacts with protein kinase-inactive mutants of IRAK1 and IRAK4. Isoform 1 interacts with isoform 2; the interaction occurs in late endosomes and disrupts the interaction between isoform 1 and TICAM1. Interacts with MYD88; the interaction decreases after IL-18 stimulation in a time-dependent manner. Interacts with IL18R1 and IL18RAP. Interacts with TLR2. Interacts with RAB11FIP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Phagocytic cup</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Late endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GIB5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001392"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001568.1">
        <property type="protein sequence ID" value="ENSGAGP00000001392.1"/>
        <property type="gene ID" value="ENSGAGG00000001122.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001891">
        <property type="term" value="C:phagocytic cup"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071651">
        <property type="term" value="P:positive regulation of chemokine (C-C motif) ligand 5 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000494">
        <property type="term" value="P:positive regulation of interleukin-18-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032481">
        <property type="term" value="P:positive regulation of type I interferon production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035666">
        <property type="term" value="P:TRIF-dependent toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000014">
        <property type="entry name" value="TIR domain-containing adapter molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046946">
        <property type="entry name" value="TCAM1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47230">
        <property type="entry name" value="TIR DOMAIN-CONTAINING ADAPTER MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47230:SF2">
        <property type="entry name" value="TIR DOMAIN-CONTAINING ADAPTER MOLECULE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15" id="KW-0391">Immunity</keyword>
    <keyword evidence="18" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="11" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0519">Myristate</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="26">
        <location>
            <begin position="63"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00056963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00063028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00072691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00080195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="217" mass="24786" checksum="E77AC51C3BF97210" modified="2019-05-08" version="1">MGNTNSKRNPPPLPNNTEKGTSVSAGQRDFKQNHELCGLSLDIERISTKVSEIHSSDEDPEDVFYKFVILHAQNDAAEASRLQHLLQNKFCIKPGIIFAEMPSGRHFLQNLNDAVNRSAWTIILLTEHFLSEAWCEFQSYASLMNALNKQHKYNSVIPLRPLNNPLPREKTPCVLQAINALEEDSPGFAKQVEKIFQESKYRQQQAIWKNERTSETH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GID4</accession>
    <name>A0A452GID4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NOTCH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GID4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001406"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001591.1">
        <property type="protein sequence ID" value="ENSGAGP00000001406.1"/>
        <property type="gene ID" value="ENSGAGG00000001121.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000136">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000327">
        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000092">
        <property type="entry name" value="Neurogenic locus notch protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000125">
        <property type="entry name" value="Neurogenic locus notch protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000095">
        <property type="entry name" value="Notch, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051830">
        <property type="entry name" value="NOTCH_homolog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24033">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24033:SF151">
        <property type="entry name" value="NOTCH 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 8" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019514576" description="EGF-like domain-containing protein" evidence="10">
        <location>
            <begin position="19"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="56"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="94"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="168"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="244"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="283"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="321"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="362"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="399"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="439"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="482"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="520"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="557"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="627"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="639"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="427"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="561"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="582"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="648" mass="66888" checksum="7C908FCDF4AF506E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJ43</accession>
    <name>A0A452GJ43_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GJ43"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001691"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001923.1">
        <property type="protein sequence ID" value="ENSGAGP00000001691.1"/>
        <property type="gene ID" value="ENSGAGG00000001358.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048599">
        <property type="term" value="P:oocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053116">
        <property type="entry name" value="GATA-type_Znf_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47341">
        <property type="entry name" value="GATA-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47341:SF1">
        <property type="entry name" value="GATA-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="4">
        <location>
            <begin position="213"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="15"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="100"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="295" mass="31855" checksum="032C04F022E924D2" modified="2019-05-08" version="1">MACCLQVPDSSALSFLQESAQRLPQAPGGQEEPSRAPRAQHQQPPAEPAVRPGRSAPQPQLGASPLASLGTCSPMDTLSLISLHCSHLVSGAAAPASLPETRAGAQTTSPCLLPKDCNGNPSREAGAAGQEGRSWEPGAGGPSQPEPSCRRKPRKQPAPSRSAEARDPSFQGVTFCMCLCLCQGSSDGYQLLIRPQYSRYRPPRSPHLTLVFSLGGKCCASCQTRKTPLWRIAEDGTLLCNACGIRYKKYRIRCFRCWNIPKKSGKPYSRCSNCGDRLRMAAAQQRTVKRSFPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLA9</accession>
    <name>A0A452GLA9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rab3-GAP regulatory subunit family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000002397"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002748.1">
        <property type="protein sequence ID" value="ENSGAGP00000002397.1"/>
        <property type="gene ID" value="ENSGAGG00000001942.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026059">
        <property type="entry name" value="Rab3GAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029257">
        <property type="entry name" value="RAB3GAP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032839">
        <property type="entry name" value="RAB3GAP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12472:SF0">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12472">
        <property type="entry name" value="RAB3-GAP REGULATORY DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14656">
        <property type="entry name" value="RAB3GAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14655">
        <property type="entry name" value="RAB3GAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab3-GAP regulatory subunit N-terminal" evidence="7">
        <location>
            <begin position="73"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Rab3GAP regulatory subunit C-terminal" evidence="8">
        <location>
            <begin position="768"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="37"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="556"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1384" mass="154744" checksum="E71A97B18B67B883" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GLH4</accession>
    <name>A0A452GLH4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">acid phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452GLH4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002643"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003021.1">
        <property type="protein sequence ID" value="ENSGAGP00000002643.1"/>
        <property type="gene ID" value="ENSGAGG00000002115.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF211">
        <property type="entry name" value="PROSTATIC ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019458113" description="acid phosphatase" evidence="8">
        <location>
            <begin position="30"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="163" mass="18688" checksum="10EA49F09B6D73CB" modified="2019-05-08" version="1">MRVRRLLCGVWSLCFLLSLFFILLHQTTAKRKLKFVSVVFRHGDQTPCESFPTDKHKKNAWPQGSGQLTKLGIQRQYELGQYMRKRYKHFLSTVYNQFEIYVQSTDADPTLMSAQASLAGLYPLAGNQVWNPKILWQPIPVHTVPVSHDKVSTNWGLSASHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMM1</accession>
    <name>A0A452GMM1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Estradiol 17-beta-dehydrogenase 11</fullName>
            <ecNumber evidence="11">1.1.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">17-beta-hydroxysteroid dehydrogenase 11</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Dehydrogenase/reductase SDR family member 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>17beta-estradiol + NAD(+) = estrone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16469"/>
            <dbReference type="ChEBI" id="CHEBI:17263"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.62"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>17beta-estradiol + NADP(+) = estrone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24616"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16469"/>
            <dbReference type="ChEBI" id="CHEBI:17263"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.62"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.62" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A452GMM1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003076"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003538.1">
        <property type="protein sequence ID" value="ENSGAGP00000003076.1"/>
        <property type="gene ID" value="ENSGAGG00000002472.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004303">
        <property type="term" value="F:estradiol 17-beta-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05339">
        <property type="entry name" value="17beta-HSDXI-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000224">
        <property type="entry name" value="Hydroxysteroid 17-beta dehydrogenase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF489">
        <property type="entry name" value="ESTRADIOL 17-BETA-DEHYDROGENASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0752">Steroid biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="302" mass="33309" checksum="52234E121AE9979E" modified="2019-05-08" version="1">MNLFMELLLFLPIIIYAYIEAFVKLFISVKRKSVSGELVLITGAGHGIGRATAYEFANRQSRLVLWDINKHGVEETAAECRRLGATAHAFVVDCSKKEEIYSAAEKVKKDIGDVTILVNNAGVIIAADLLSTQDHQIQKMFEVNILAHYWTAKAFLPAMMNNNHGHIVTVASAAGHLATSFMVTYSSSKFAAVGFHKALTQELSALGKDGIKTSCLCPAFVNTGFVKNPSMSLMKVLEPEKVANKLLEGILTNQKMIFIPSFLKIHLILEKILPEHALAALYKLQNIQFDAVVGYRNRVISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMQ3</accession>
    <name>A0A452GMQ3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the small GTPase superfamily. RasD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GMQ3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003108"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003582.1">
        <property type="protein sequence ID" value="ENSGAGP00000003108.1"/>
        <property type="gene ID" value="ENSGAGG00000002511.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000475">
        <property type="entry name" value="GTP-binding protein Rhes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052236">
        <property type="entry name" value="Small_GTPase_RasD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149">
        <property type="entry name" value="MIP08469P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149:SF8">
        <property type="entry name" value="NOVEL MEMBER OF RAS FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="206" mass="22563" checksum="D54E7906A0EE9567" modified="2019-05-08" version="1">MALCPAPEARVRLVFFGAAGVGKTALIRRFLQDSFEARHRRTVEELHLLELELGAGSGLRLRLEILDTSGSYSFPAMRRLCIGRADAFALVYSLQEPDSFAEVQRLRDEILEAKRGAVPVVVVGNKSDLAPGEPDPRIAAAQRDWGCTCLEASAKQGRNVVGLFQELLSQVNLPGRLSPALQRRQPQTCSKEPPRRSSKSHGCAVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GMY7</accession>
    <name>A0A452GMY7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Galectin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GMY7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003232"/>
    <dbReference type="Ensembl" id="ENSGAGT00000003728.1">
        <property type="protein sequence ID" value="ENSGAGP00000003232.1"/>
        <property type="gene ID" value="ENSGAGG00000002616.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030395">
        <property type="term" value="F:lactose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043236">
        <property type="term" value="F:laminin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000021">
        <property type="entry name" value="Galectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF97">
        <property type="entry name" value="GALECTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Galectin" evidence="3">
        <location>
            <begin position="4"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="135" mass="14983" checksum="961BA486B63628C1" modified="2019-05-08" version="1">MECGLVATHLNIQAGECIKVKGKILPEAKGFAVNVGKDRSNLVLHFNPRFDSHGDVNVIVCNSMEDGMWGTEERETDFPFQQGDKTEICISFDTAELTVKLAGDKKIMFPNRLGLENIEYLSVEGDFAIKAIKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GP83</accession>
    <name>A0A452GP83_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GP83"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003709"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004290.1">
        <property type="protein sequence ID" value="ENSGAGP00000003709.1"/>
        <property type="gene ID" value="ENSGAGG00000003011.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="293" mass="29194" checksum="84E91D94740F594A" modified="2019-05-08" version="1">MEAPALGWGGVNSMTLPGTPGRTLAHTQPKRLPLPRPQRLSPNYHPLRPISHTHAGRAGSCLPHPGEGMGREHWAPWGDSLQLGPLRLPSRGTATASSQHYPGPQRPHPSAKPACDCDVPAPGAKPACDCDVPAPGAKPACDVPAPGVNPACNAPVPAPGANPTCNAPIPAPSAKPACDVPAPGVNPACNAPIPAPSTKPACDVPAPALGTNPACNAPIPAPSAKPACDVPAPGVNPACNAPIPAPSTKPACDIPAPGVNLACNAPIPALGTNPACNAPVPAQSRPATSQRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GP86</accession>
    <name>A0A452GP86_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GP86"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003492"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004026.1">
        <property type="protein sequence ID" value="ENSGAGP00000003492.1"/>
        <property type="gene ID" value="ENSGAGG00000002819.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="68"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="126" mass="13971" checksum="A0A1D0EB83B6651E" modified="2019-05-08" version="1">DECLYESFPEVPLEEMSEADGEASKLSLSVQEPSSPATSSEAFTPKEGSPYKAPIYIPDNIPIPSEFELRESNVPGAGLGIWTKRKIEVGEKFGPYVGEQQSNLKDPSYGWEVRNFLNSFVLLNCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPW9</accession>
    <name>A0A452GPW9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SH3 and cysteine-rich domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <location evidence="11">Sarcolemma</location>
            <location evidence="11">T-tubule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GPW9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003870"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004491.1">
        <property type="protein sequence ID" value="ENSGAGP00000003870.1"/>
        <property type="gene ID" value="ENSGAGG00000003161.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030315">
        <property type="term" value="C:T-tubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903078">
        <property type="term" value="P:positive regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11986">
        <property type="entry name" value="SH3_Stac3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11834">
        <property type="entry name" value="SH3_Stac_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000022">
        <property type="entry name" value="SH3 and cysteine-rich domain-containing protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000167">
        <property type="entry name" value="SH3 and cysteine-rich domain-containing protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039688">
        <property type="entry name" value="STAC1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035736">
        <property type="entry name" value="Stac3_SH3_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135:SF2">
        <property type="entry name" value="SH3 AND CYSTEINE-RICH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135">
        <property type="entry name" value="STAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16664">
        <property type="entry name" value="STAC2_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="151"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="211"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="105"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="268" mass="30443" checksum="2806B557A011594E" modified="2019-05-08" version="1">GPLLPAELVPWLGRLTDLSLPPTPPPVNNKFGLRCKNCKTSIHQHCQSYVEMQRCFGKMPPGFRRAYSSPLYSNQQYACVKELLSAANRSDPVFETLRTGVIMANKERKKGQDDKKNVSWREAPGGPCNPEGDKKAEKSTSDDKKPQPGFLQTHYFVALYRFKALEKDDLDFHPGEKITVVDDSNEEWWRGKIGEKIGYFPPNFIIRVRAGERVHKVTRSFVGNKEIGQITLKKDQIVVQKGEEVNGYVKVYTGRKVGLFPVDFLEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQR9</accession>
    <name>A0A452GQR9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GQR9"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004710.1">
        <property type="protein sequence ID" value="ENSGAGP00000004038.1"/>
        <property type="gene ID" value="ENSGAGG00000003307.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007342">
        <property type="term" value="P:fusion of sperm to egg plasma membrane involved in single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031684">
        <property type="entry name" value="LLCFC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37348">
        <property type="entry name" value="LLLL AND CFNLAS MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37348:SF1">
        <property type="entry name" value="SPERM-EGG FUSION PROTEIN LLCFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15838">
        <property type="entry name" value="LLCFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="102" mass="11397" checksum="3AC2EDC7FBE04CF8" modified="2019-05-08" version="1">SMRPCSGPGPLKRQRGELMSPSWVTGWEQMEETFVASSTGEGMELIDMVQNEDAQRLRNSVVKDLLLDLAISLNTGSIMLFFLCHRTSPNNSCFLEQHPALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRC4</accession>
    <name>A0A452GRC4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GRC4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004512"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005288.1">
        <property type="protein sequence ID" value="ENSGAGP00000004512.1"/>
        <property type="gene ID" value="ENSGAGG00000003732.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09805">
        <property type="entry name" value="type2_17beta_HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000074">
        <property type="entry name" value="Retinol dehydrogenase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313:SF15">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY 9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018978049" evidence="4">
        <location>
            <begin position="21"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="322" mass="35902" checksum="789F7F30CF35D564" modified="2019-05-08" version="1">MFFYILIFLAIFYLWWRCGAQDGQKIRDLTDKYIFITGCDSGFGNLSARTFDKKGFRVFASCLTEVGAVELKAATSDRLQTVLLDVTDPDNVRKVAEWIKAEVGTAGLWGLVNNAGIMGPSAPTDWLNIEHFRAPIEINLIGLINVTLHLLPLVKKARGRLVNISSTGGRLAVYGGGYFPSKFGVEAFNDSLRRDMKAFGVKVSCIEPGLFKTGLSNRKKIIEEREVIWNQLPPAIRKQYGEGYVQKDAARREKLIQVLQNTNLSLVVECMEHALTSINPRSRYTAGWDAKLLWIPLSSMPAAIQDFVLLRNKTELADPTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GT02</accession>
    <name>A0A452GT02_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the NPY family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GT02"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005024"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005864.1">
        <property type="protein sequence ID" value="ENSGAGP00000005024.1"/>
        <property type="gene ID" value="ENSGAGG00000004104.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031841">
        <property type="term" value="F:neuropeptide Y receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007631">
        <property type="term" value="P:feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00126">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001955">
        <property type="entry name" value="Pancreatic_hormone-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020392">
        <property type="entry name" value="Pancreatic_hormone-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10533">
        <property type="entry name" value="NEUROPEPTIDE Y/PANCREATIC HORMONE/PEPTIDE YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10533:SF14">
        <property type="entry name" value="PEPTIDE YY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00159">
        <property type="entry name" value="Hormone_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00278">
        <property type="entry name" value="PANCHORMONE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00309">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00265">
        <property type="entry name" value="PANCREATIC_HORMONE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50276">
        <property type="entry name" value="PANCREATIC_HORMONE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019230379" evidence="5">
        <location>
            <begin position="29"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="97" mass="10915" checksum="2FBBEFA891C7544D" modified="2019-05-08" version="1">MVASAKLWPMLVAVTLCVLSCLGTLVEAYPPKPENPGDNASPEEMAKYFSALRHYINLVTRQRYGKRSSPEALMSELLFGDSSDHTGRSRYEDSYLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GV18</accession>
    <name>A0A452GV18_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GV18"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005963"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006950.1">
        <property type="protein sequence ID" value="ENSGAGP00000005963.1"/>
        <property type="gene ID" value="ENSGAGG00000004848.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15225">
        <property type="entry name" value="7tmA_OR10A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="44"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="320" mass="36467" checksum="20CF0EB47FCF5620" modified="2019-05-08" version="1">SENMNMGNLTTVTEFILLGFSNHHDLQVPLFSIYLFIYIITLMGNMLIILITIDTALQTPMYFFLRVLSFLEICYTSVTIPKMLVDFLSDNRSISYLGCAAQMYFLLFLGISECFLLAAMAYDRYVAICNPLRYRLIMNRRVCLFLTVLSWFWGNVVSLVQTAWVFTLPFCGSNQINYFFCDIPPLIKLSCTDTSSYEMQLFTVTILVNFTPFSLIVVSYTLIISTILKMASADGRHKAFSTCSSHLIVVTLYYGSSGLIYLRPKSINSPDSNKVLALMYTTITPILNPIVYSLRNNEVKGAMQRLLWNGLKGKIFSQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GVI4</accession>
    <name>A0A452GVI4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BMP/retinoic acid-inducible neural-specific protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRINP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GVI4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006134"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007143.1">
        <property type="protein sequence ID" value="ENSGAGP00000006134.1"/>
        <property type="gene ID" value="ENSGAGG00000004966.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071300">
        <property type="term" value="P:cellular response to retinoic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045930">
        <property type="term" value="P:negative regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045666">
        <property type="term" value="P:positive regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033237">
        <property type="entry name" value="BRINP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057671">
        <property type="entry name" value="BRINP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057450">
        <property type="entry name" value="BRINP_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15564:SF7">
        <property type="entry name" value="BMP_RETINOIC ACID-INDUCIBLE NEURAL-SPECIFIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15564">
        <property type="entry name" value="MACPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19052">
        <property type="entry name" value="BRINP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25415">
        <property type="entry name" value="EGF_BRNP1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0338">Growth arrest</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019585619" description="BMP/retinoic acid-inducible neural-specific protein 1" evidence="10">
        <location>
            <begin position="21"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="11">
        <location>
            <begin position="72"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="761" mass="88921" checksum="6F06B638CB2CB213" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWL7</accession>
    <name>A0A452GWL7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Minus-end microtubule-dependent motor protein. Involved in apically targeted transport. Required for zonula adherens maintenance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16 17">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GWL7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006412"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007463.1">
        <property type="protein sequence ID" value="ENSGAGP00000006412.1"/>
        <property type="gene ID" value="ENSGAGG00000005183.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01366">
        <property type="entry name" value="KISc_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000022">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF43">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="10 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12 16 17" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="19">
        <location>
            <begin position="511"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="103"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="264"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="594"/>
            <end position="601"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="844" mass="95399" checksum="9B43C1F356296586" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYD4</accession>
    <name>A0A452GYD4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Short chain dehydrogenase/reductase family 16C member 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GYD4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007171"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008297.1">
        <property type="protein sequence ID" value="ENSGAGP00000007171.1"/>
        <property type="gene ID" value="ENSGAGG00000005761.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05339">
        <property type="entry name" value="17beta-HSDXI-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000202">
        <property type="entry name" value="Short-chain dehydrogenase/reductase family 16C member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF749">
        <property type="entry name" value="EPIDERMAL RETINOL DEHYDROGENASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="35326" checksum="C33292EDE0179EB3" modified="2019-05-08" version="1">MCKLSSILETLEFLILLGYYCFEAFILFICPPCKKNVAGEIVLITGAANGIGRQVALNFARLGATLIFWDIDEEGNKETIKLAKENGAKGVYAYRCDCSNREEVYRVADQVRKEVGDVNILVNNAGIANMKTFLDLPDADMEKTMRVNIMAQFWTCKAFLPAMIACNRGHLVSISSGIGLIGTNKMTDYSASKFAIIGFLESLTFELQAAGKKGIKTTIVCPSFINTELSTGIRVSRPLLIPILDVEYASRKIVDAIREEKFYVFMPLTNCIAALKNFVPKKVLLLAVEYAGIFNNLDGVGDRKRRDQNHTALKMSDTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GYD9</accession>
    <name>A0A452GYD9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methionyl-tRNA formyltransferase, mitochondrial</fullName>
            <ecNumber evidence="3">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Methionyl-tRNA formyltransferase that formylates methionyl-tRNA in mitochondria and is crucial for translation initiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-methionyl-tRNA(fMet) + (6R)-10-formyltetrahydrofolate = N-formyl-L-methionyl-tRNA(fMet) + (6S)-5,6,7,8-tetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9953"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:78844"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:24381"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Fmt family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452GYD9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007076"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008194.1">
        <property type="protein sequence ID" value="ENSGAGP00000007076.1"/>
        <property type="gene ID" value="ENSGAGG00000005684.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12230:FF:000003">
        <property type="entry name" value="methionyl-tRNA formyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005794">
        <property type="entry name" value="Fmt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00460">
        <property type="entry name" value="fmt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138:SF5">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="11">
        <location>
            <begin position="99"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl transferase C-terminal" evidence="12">
        <location>
            <begin position="228"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="375" mass="41877" checksum="B70913F97EEC7BC6" modified="2019-05-08" version="1">MRGRLLRAWREGAAARGLAELGAPRGKPPWRVLFFGTDDFAAESLRALEAAREPREEPLIDRLEVVTLPSPLPRGPPVKNCAQQFQLPTHHWPDTGPSSQFDVGVVASFGRLLNEDLILQFPYGVLNVHPSYLPRWRGPAPIVHAVLHGDTVTGVTIMQIKPKRFDVGPIIKQERYAVPSQCTAKELEAILSKLGANMLISVLKNLPESLKNKREQPNEGVTFAPKVTIAMSCIKWEEQTPEQILRLHRAIGATLPLQTLWMGNTVKLLDFVEVHNICSFADQLLNEHGIVPGSVLYHKSSETLVVRCKEGWVGVKTVILKKKLTAADFYNGYLHAWFQQKTLHGDCRFHTLKLTTVKKTERKGNGGAQYKTKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZP4</accession>
    <name>A0A452GZP4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Shisa N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GZP4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007665"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008847.1">
        <property type="protein sequence ID" value="ENSGAGP00000007665.1"/>
        <property type="gene ID" value="ENSGAGG00000006138.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000008849.1">
        <property type="protein sequence ID" value="ENSGAGP00000007667.1"/>
        <property type="gene ID" value="ENSGAGG00000006138.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053891">
        <property type="entry name" value="Shisa_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13908">
        <property type="entry name" value="Shisa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044604465" description="Shisa N-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Shisa N-terminal" evidence="4">
        <location>
            <begin position="31"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="211" mass="23125" checksum="98EC2D9C8AD9681C" modified="2019-05-08" version="1">MLHRLLLGGLLCTTLPTSSLGTASAHNLHLCEGYPSPDGRYHSGFYCPRLTDPPAHRYCCHHGDHTLKSCCPQLDFESLRRVNLSNVPTPARLRNPLPLLAVGLYGLLVLTLMTLDFLHFCRLNQCRVYSLLSSSRLGKHLANSFPHRCPRPGLARGGTQRQELPTGGGTQRQELPTAWPGPAGGGTQWQVLAVTTARPSQDSKCEEQAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3S3</accession>
    <name>A0A452H3S3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Decapping nuclease</fullName>
            <ecNumber evidence="5">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end CoA-ribonucleoside in mRNA + H2O = 3'-dephospho-CoA + a 5'-end phospho-ribonucleoside in mRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67496"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:172371"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:67497"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 5'-end FAD-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + FAD + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67492"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17275"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:172372"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:67493"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in snoRNA + H2O = a 5'-end phospho-ribonucleoside in snoRNA + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60892"/>
            <dbReference type="Rhea" id="RHEA-COMP:15699"/>
            <dbReference type="Rhea" id="RHEA-COMP:15700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:60893"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 2 magnesium ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DXO/Dom3Z family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452H3S3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009094"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010435.1">
        <property type="protein sequence ID" value="ENSGAGP00000009094.1"/>
        <property type="gene ID" value="ENSGAGG00000007179.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034353">
        <property type="term" value="F:mRNA 5'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110155">
        <property type="term" value="P:NAD-cap decapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013961">
        <property type="entry name" value="RAI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039039">
        <property type="entry name" value="RAI1-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395:SF9">
        <property type="entry name" value="DECAPPING AND EXORIBONUCLEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395">
        <property type="entry name" value="DOM-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08652">
        <property type="entry name" value="RAI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RAI1-like" evidence="7">
        <location>
            <begin position="238"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="101"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="356" mass="37546" checksum="032F6C7FD2763037" modified="2019-05-08" version="1">ARGRPRAPDQQGALGGSLLNSGAPRERRFRDVAGAPYQGADDALREPGGLAAGRQPLPGHPVRERGGDAGGPAAAGAALRHAAGAGLHGLQVRALHVCGHPGREPGPHGGGEHQRGVLHGGAHAAGRALAALRWGGGLHRPPRGLAQAPRLLRGAQDVQGAAQPHSAQELLQVRAPSRPASQLCPKEPPSLRLPCVYVLCGARQVVGGLSPVFPAWGGGREAGGYSGPRLMPPSLPPRHKLIKWWAQSFLPGVSRIVAGFRAPDGAVAALETFETMKIFQLIRGDRGCWKPAVCMNFCEAFLAFLKRVVTEDDPQLVHLFAWEPGSPVSYTRHRAAEHVVLPAWYVETMSGGKKPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H4D1</accession>
    <name>A0A452H4D1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H4D1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000009340"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010730.1">
        <property type="protein sequence ID" value="ENSGAGP00000009340.1"/>
        <property type="gene ID" value="ENSGAGG00000007388.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17682">
        <property type="entry name" value="RUN_RUFY4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46753">
        <property type="entry name" value="FYVE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46753:SF3">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="4">
        <location>
            <begin position="26"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="175"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="2">
        <location>
            <begin position="304"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="51890" checksum="C0392D884B84BD26" modified="2019-05-08" version="1">MTGKEPLLGTLKACILAIKEGSSPCTDASLHLAPFCEILETILRKGIKQLVLGFKRRDYWHWLEQLPQQDTCSGVTPLSIMVEKASSCEKVLTAQGRGRYFLRLALNQKSLATAIQQLARSPKLLECYDPLVSVLGNEDFMEPFLSLMLVVSEMNFSLDLQNSSFLDESWLLPVCVTYETVPCRELGMVLRYLDGRIFVIEVLPESQAEVDEVVLVGDVIDEISGFSLRNAHNGQAGTVLQKLKGKPLSFRIIRWKWHDGGMYRPLLPYLKVLREKVPSFQLQQEHRHKEEKEERCLQGGRLLYNLRYLGQANVGKYGGKEVLDEGIPTVLEQHLCPREVLFDVKETEVFIQEKTSSKVLFQYPYPEISCVGRRLDSNNLFAFCVAVSPGTPEHSTFDCLVFESNSENECEEIIKRIGLKRSYPAVTGNETKTKTSYDGKAHMQNSKDTRIYSDTYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H5X3</accession>
    <name>A0A452H5X3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Neurogenic differentiation factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H5X3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010066"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011552.1">
        <property type="protein sequence ID" value="ENSGAGP00000010066.1"/>
        <property type="gene ID" value="ENSGAGG00000007901.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19719">
        <property type="entry name" value="bHLH_TS_NeuroD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000006">
        <property type="entry name" value="Neurogenic differentiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022575">
        <property type="entry name" value="NeuroD_DUF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016637">
        <property type="entry name" value="TF_bHLH_NeuroD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF88">
        <property type="entry name" value="NEUROGENIC DIFFERENTIATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12533">
        <property type="entry name" value="Neuro_bHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015618">
        <property type="entry name" value="bHLH_NeuroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 11" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="104"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR015618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="357" mass="40325" checksum="AF39323E543CB5C0" modified="2019-05-08" version="1">MTKSYSESGLMGEPQPQGPPSWTDECLSSQDEEHEADKKEEDLQAMQAENEEDSLRNGEEEDEEDDLEEEEEEEEEEEDDDQKPKRRGPKKKKMTKARMERFKLRRMKANARERNRMHGLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEILRSGKSPDLVSFVQTLCKGLSQPTTNLVAGCLQLNPRTFLPEQSQDMPPHMQPASASFPVHPYAYQSPGLPSPPYGTMDSSHIFHMKPRHSFEAALEPFFDSTLTDCTSPSFDGPLSPPLSINGNFSFKHEPSNEFDKNYAFTMHYPAATLAGASGHGSIFSGSASRCEIPIDNIMPYESHSHHERVMSAQLNAIFHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H745</accession>
    <name>A0A452H745_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL52</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L52, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL52 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H745"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012076.1">
        <property type="protein sequence ID" value="ENSGAGP00000010516.1"/>
        <property type="gene ID" value="ENSGAGG00000008227.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034596">
        <property type="entry name" value="Ribosomal_mL52"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34090">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L52, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34090:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18699">
        <property type="entry name" value="MRPL52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="87" mass="9937" checksum="52AEA31889602A4E" modified="2019-05-08" version="1">LAAYDGELISVHQYHISCAQWKIQLGLDPSLEGYGCLSDLPDWSFVDWCPTPSGKGQSHWKQDNDAFVRLRTFMHVSSKWDYLCACL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H8Q1</accession>
    <name>A0A452H8Q1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A452H8Q1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011115"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012727.1">
        <property type="protein sequence ID" value="ENSGAGP00000011115.1"/>
        <property type="gene ID" value="ENSGAGG00000008599.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008593">
        <property type="term" value="P:regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000053">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF63">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0430">Lectin</keyword>
    <keyword evidence="9 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="13">
        <location>
            <begin position="154"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="522" mass="59947" checksum="06014E8E227DB6F7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H9C6</accession>
    <name>A0A452H9C6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H9C6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011177"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012800.1">
        <property type="protein sequence ID" value="ENSGAGP00000011177.1"/>
        <property type="gene ID" value="ENSGAGG00000008647.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019351">
        <property type="entry name" value="DUF2039"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22876:SF5">
        <property type="entry name" value="CHROMOSOME 9 OPEN READING FRAME 85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22876">
        <property type="entry name" value="ZGC:101016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10217">
        <property type="entry name" value="DUF2039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="150" mass="17504" checksum="D87BEB51835483FD" modified="2019-05-08" version="1">TSHLSRARSKINAKLHDGVCQRCKEVLEWRVKFSKYKPLSKPKKCVKCLQKTVKDSYHIICRSCACELEVCAKCGKKEEIVVLISKGPEKMENETTGNCQRKISRREESEESDELDFDIDLDGMDEKNPDMLEEHFKNMKFERTEVKLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HA83</accession>
    <name>A0A452HA83_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 17">Alkaline phosphatase</fullName>
            <ecNumber evidence="4 17">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="4 17"/>
    <dbReference type="AlphaFoldDB" id="A0A452HA83"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013327.1">
        <property type="protein sequence ID" value="ENSGAGP00000011632.1"/>
        <property type="gene ID" value="ENSGAGG00000008902.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000008">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF30">
        <property type="entry name" value="INTESTINAL-TYPE ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 15 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15 17" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Phosphoserine intermediate" evidence="14">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="522" mass="57102" checksum="78EBCAB09F7B1364" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAS3</accession>
    <name>A0A452HAS3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HAS3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011843"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013562.1">
        <property type="protein sequence ID" value="ENSGAGP00000011843.1"/>
        <property type="gene ID" value="ENSGAGG00000009127.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000966">
        <property type="entry name" value="ZFP90 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000269">
        <property type="entry name" value="Zinc finger protein 287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000069">
        <property type="entry name" value="Zinc finger protein 572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000431">
        <property type="entry name" value="zinc finger protein 629 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF416">
        <property type="entry name" value="FI01424P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="298" mass="33733" checksum="C04F50DE2A8AEDC8" modified="2019-05-08" version="1">MVSENEEEKPQQEDAVQVEPRGTLSGRSKGSVSRSCAFSQNTKACETQQRPEENYSSHSDLLTCGRINLEERRYACHECGKSFNRSSHLLRHQRIHTGELPFTCAECGKSFNQSSALSTHRRIHTGEKPYGCAECGKSFNQSSDLIRHQRIHTGETPYTCSECGKRFSQSSALSTHRRIHTGEKPYGCVECGKSFNQSSNLIAHRRIHTCEKPYGCSDFSRRSRLITHQRIHIGETPYTCSECGKSFSQSSALSAHRRIHTGEKLYTCSECGKSFKQSPSLIRHHKIHLRVNCYKSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBA4</accession>
    <name>A0A452HBA4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HBA4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012039"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013788.1">
        <property type="protein sequence ID" value="ENSGAGP00000012039.1"/>
        <property type="gene ID" value="ENSGAGG00000009272.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000355">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 20 isoform X1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000004">
        <property type="entry name" value="zinc finger protein 184 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000506">
        <property type="entry name" value="Zinc finger protein 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000902">
        <property type="entry name" value="Zinc finger protein 445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF416">
        <property type="entry name" value="FI01424P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="13">
        <location>
            <begin position="30"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="512"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="540"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="568"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="596"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="624"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="657"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="707" mass="80209" checksum="364B0E1D3C1CACF7" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBZ4</accession>
    <name>A0A452HBZ4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Keratin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the avian keratin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HBZ4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012285"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014070.1">
        <property type="protein sequence ID" value="ENSGAGP00000012285.1"/>
        <property type="gene ID" value="ENSGAGG00000009451.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003461">
        <property type="entry name" value="Keratin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31203">
        <property type="entry name" value="BETA-KERATIN-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31203:SF1">
        <property type="entry name" value="BETA-KERATIN-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02422">
        <property type="entry name" value="Keratin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0416">Keratin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="10654" checksum="5B03EA4C0F5E06EE" modified="2019-05-08" version="1">LHHRKMSCSSLSYPECGVARPSPVSGSCNEPCIRQCPDSEVLIQPSPVVVTIPGPILSTFPQQSEVGAVGAPVVGSGYGGSFGLGGLYGYGALWVQGTTLCKAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HCU2</accession>
    <name>A0A452HCU2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein-lysine N-trimethyltransferase SMYD5</fullName>
            <ecNumber evidence="2">2.1.1.359</ecNumber>
            <ecNumber evidence="10">2.1.1.372</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">SET and MYND domain-containing protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">[histone H3]-lysine20 N-trimethyltransferase SMYD5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">[histone H4]-lysine36 N-trimethyltransferase SMYD5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl(20)-[histone H4] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(20)-[histone H4] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:64456"/>
            <dbReference type="Rhea" id="RHEA-COMP:15554"/>
            <dbReference type="Rhea" id="RHEA-COMP:15998"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.372"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60324"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:15536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:54193"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.359" evidence="2"/>
    <dbReference type="EC" id="2.1.1.372" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A452HCU2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012630"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014482.1">
        <property type="protein sequence ID" value="ENSGAGP00000012630.1"/>
        <property type="gene ID" value="ENSGAGG00000009701.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140955">
        <property type="term" value="F:histone H3K36 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140943">
        <property type="term" value="F:histone H4K20 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10521">
        <property type="entry name" value="SET_SMYD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000078">
        <property type="entry name" value="SMYD family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044422">
        <property type="entry name" value="SMYD5_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46402:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-TRIMETHYLTRANSFERASE SMYD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46402">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="19">
        <location>
            <begin position="21"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="392"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="416" mass="47442" checksum="B8E46E93AE3B31EE" modified="2019-05-08" version="1">MAASMAELFCFCAVPAGAARATVEVRFISSLKGKGLFATRNIHKGETIFLEKPVVSSQFLWNALYRYKACDHCLRALETAEENAQRLLGKSCRVLPHPEQCSIRKDLHQHCPHCQVAYCSAECRHSAFEQYHQVLCLGPSREDPKHPLNKLQDTWRNIHYPPETSSIMLLARMVATVKQAKDKDWWIKLFSQFCSKTANEEEEIVHKLLGDKFKGQLELLRQLFTEALYEERLSRWFTPEGFQSLFALVGTNGQGIGTSSLSQWVHACDALELPPHEREQLDAFIDQLYKDIEKETGEFLNCEGSGLYVLQSCCNHSCIPNAETSFPDNNFLLHLIALEEIKPGEEICISYLDCCQRERSRHSRHKILREHYLFTCSCPKCLAQADDANLTSEEEEELEGEGETDDAELEDEMTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HD59</accession>
    <name>A0A452HD59_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ectonucleotide pyrophosphatase/phosphodiesterase 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HD59"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012756"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014621.1">
        <property type="protein sequence ID" value="ENSGAGP00000012756.1"/>
        <property type="gene ID" value="ENSGAGG00000009785.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF63">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019578588" description="Ectonucleotide pyrophosphatase/phosphodiesterase 7" evidence="1">
        <location>
            <begin position="18"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="9913" checksum="0269FA52C50EFCBE" modified="2019-05-08" version="1">MFTSLGLVLAVLSLGACAPLQDRGNHSKVLLVSFDGFRWNYDQDVETPNLDAMARDGVKARYMTPAFITLTSPCHFTLLTGKRLETHSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFS5</accession>
    <name>A0A452HFS5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 11 12">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000013698"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015690.1">
        <property type="protein sequence ID" value="ENSGAGP00000013698.1"/>
        <property type="gene ID" value="ENSGAGG00000009780.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016486">
        <property type="term" value="P:peptide hormone processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000006">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000002">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032778">
        <property type="entry name" value="GF_recep_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF8">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14843">
        <property type="entry name" value="GF_recep_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50900">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 11 12" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 11 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019020364" evidence="14">
        <location>
            <begin position="19"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="16">
        <location>
            <begin position="450"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="PLAC" evidence="15">
        <location>
            <begin position="849"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="167"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="887" mass="98517" checksum="9040B3066FAD5509" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HFT6</accession>
    <name>A0A452HFT6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Intraflagellar transport protein 140 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">WD and tetratricopeptide repeats protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs). Plays a pivotal role in proper development and function of ciliated cells through its role in ciliogenesis and/or cilium maintenance. Required for the development and maintenance of the outer segments of rod and cone photoreceptor cells. Plays a role in maintenance and the delivery of opsin to the outer segment of photoreceptor cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the IFT complex A (IFT-A). IFT-A complex is divided into a core subcomplex composed of IFT122:IFT140:WDR19 which is associated with TULP3 and a peripheral subcomplex composed of IFT43:WDR35:TTC21B. Interacts (via C-terminal region) with IFT122 (via C-terminal region). Interacts with TTC25. Interacts with TTC21A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000013730"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015724.1">
        <property type="protein sequence ID" value="ENSGAGP00000013730.1"/>
        <property type="gene ID" value="ENSGAGG00000010466.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030991">
        <property type="term" value="C:intraciliary transport particle A"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000675">
        <property type="entry name" value="Intraflagellar transport 140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000811">
        <property type="entry name" value="Intraflagellar transport 140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000010">
        <property type="entry name" value="Intraflagellar transport 140 homolog (Chlamydomonas)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000011">
        <property type="entry name" value="Intraflagellar transport protein 140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056154">
        <property type="entry name" value="Beta-prop_IFT140_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056155">
        <property type="entry name" value="Beta-prop_IFT140_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056168">
        <property type="entry name" value="TPR_IF140/IFT172/WDR19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056156">
        <property type="entry name" value="TPR_IF140_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722">
        <property type="entry name" value="IFT140/172-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722:SF7">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 140 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23383">
        <property type="entry name" value="Beta-prop_IFT140_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23385">
        <property type="entry name" value="Beta-prop_IFT140_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24762">
        <property type="entry name" value="TPR_IF140-IFT172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24760">
        <property type="entry name" value="TPR_IF140_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
        <property type="entry name" value="Putative isomerase YbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5 16" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="IFT140 first beta-propeller" evidence="18">
        <location>
            <begin position="2"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="99"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="IFT140 second beta-propeller" evidence="19">
        <location>
            <begin position="416"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="domain" description="IF140/IFT172/WDR19 TPR" evidence="21">
        <location>
            <begin position="784"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="domain" description="IF140 C-terminal TPR" evidence="20">
        <location>
            <begin position="1278"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1435"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1435"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1447"/>
            <end position="1460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF23383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF23385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF24760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF24762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1460" mass="165296" checksum="897DA1874DAE30F2" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGB6</accession>
    <name>A0A452HGB6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGB6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013905"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015917.1">
        <property type="protein sequence ID" value="ENSGAGP00000013905.1"/>
        <property type="gene ID" value="ENSGAGG00000010591.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01469">
        <property type="entry name" value="vWA_integrins_alpha_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.930:FF:000005">
        <property type="entry name" value="Integrin, alpha 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000012">
        <property type="entry name" value="Integrin, alpha 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF21">
        <property type="entry name" value="INTEGRIN ALPHA-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 16" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0401">Integrin</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1040"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="62"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="253"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="362"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="426"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="488"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1086" mass="122394" checksum="340DF41D679F10B4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGK4</accession>
    <name>A0A452HGK4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin light chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasmic vesicle membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
            <orientation evidence="1 6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="6">Cytoplasmic face of coated pits and vesicles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the clathrin light chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGK4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013996"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016017.1">
        <property type="protein sequence ID" value="ENSGAGP00000013996.1"/>
        <property type="gene ID" value="ENSGAGG00000010657.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099631">
        <property type="term" value="C:postsynaptic endocytic zone cytoplasmic component"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000996">
        <property type="entry name" value="Clathrin_L-chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10639">
        <property type="entry name" value="CLATHRIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10639:SF1">
        <property type="entry name" value="CLATHRIN LIGHT CHAIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01086">
        <property type="entry name" value="Clathrin_lg_ch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00581">
        <property type="entry name" value="CLATHRIN_LIGHT_CHN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="104" mass="12046" checksum="39048D431345BC68" modified="2019-05-08" version="1">MERGAKGTPRNANSRKQEAEWKEKAIKELEEWYARQDEQLQKTKANNRAAEEAFVNDVEEISPGTEWERVARLCDFNPKSSKQAKDVSRMRSILISLKQAPLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGQ0</accession>
    <name>A0A452HGQ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HGQ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014051"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016090.1">
        <property type="protein sequence ID" value="ENSGAGP00000014051.1"/>
        <property type="gene ID" value="ENSGAGG00000010704.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="383" mass="43601" checksum="1E78C3BB0F3CF9F8" modified="2019-05-08" version="1">MDIVGPLSKTTRSGKKYILVVVDFATRYPEAVALSSIEADTVADALLTIFSRVGFPKEVLTDQGSNFMSALLRSLWQKCGVQHNWASAYHPQSNGLVERFNGTLKMMLKTFMNQHPQDWDKYLPHLLFAYREVPQESTGFSPFELLYGRRVRGPLDLMRDEWEGKAAPEGESVVEYVLTFRERLAELMGLARENLARAQRRQKVWYDRTARARAFATGDQVMVLIPVRRNKLQAAWEGPFKVIKQLNEVNYVVELSNRAHHRRVYHVNMMKPYYDRGNVVLAVCGHWEGQGDDPLVDLFPGTKAGSPLEAIPLSEQLTPGQHAEIRGVLHLYRQLFSNQPGRTNLTVHRVETGSHAPIRCSPFRVTGKTAQDLEREVRDMLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHM3</accession>
    <name>A0A452HHM3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Interleukin-6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-6 superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HHM3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014346"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016414.1">
        <property type="protein sequence ID" value="ENSGAGP00000014346.1"/>
        <property type="gene ID" value="ENSGAGG00000010905.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005896">
        <property type="term" value="C:interleukin-6 receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005138">
        <property type="term" value="F:interleukin-6 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046427">
        <property type="term" value="P:positive regulation of receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003574">
        <property type="entry name" value="IL-6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030474">
        <property type="entry name" value="IL-6/GCSF/MGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030473">
        <property type="entry name" value="IL6/GCSF/MGF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48494">
        <property type="entry name" value="INTERLEUKIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48494:SF1">
        <property type="entry name" value="INTERLEUKIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00489">
        <property type="entry name" value="IL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00433">
        <property type="entry name" value="IL6GCSFMGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00434">
        <property type="entry name" value="INTERLEUKIN6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00126">
        <property type="entry name" value="IL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00254">
        <property type="entry name" value="INTERLEUKIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0011">Acute phase</keyword>
    <keyword evidence="5" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0339">Growth factor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019490550" description="Interleukin-6" evidence="10">
        <location>
            <begin position="20"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="214" mass="24073" checksum="F920713644E50778" modified="2019-05-08" version="1">FSGFLLAAAALTLLLGTRAVPLPDSSGEEELVDPPDSLARSPLLPDCESLAWLLHSKAAKLKDEMCEKFTVCDNSMEMLAQNNLNLPKITEEDGCLLSGFNEEKCLRGISSGLFAFQTYLEYVRETFISEKQKVESICYGTKHLANTMRQMMKNPDAVIVPDPATQSTLFEKLKSNKKWIEKISTHLILRDFTSFMEKTVRAVRYLKNTRNLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HHT6</accession>
    <name>A0A452HHT6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000014415"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016487.1">
        <property type="protein sequence ID" value="ENSGAGP00000014415.1"/>
        <property type="gene ID" value="ENSGAGG00000010917.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23463">
        <property type="entry name" value="beta-trefoil_Ricin_vlAKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000006">
        <property type="entry name" value="Very large A-kinase anchor protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000008">
        <property type="entry name" value="very large A-kinase anchor protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000009">
        <property type="entry name" value="very large A-kinase anchor protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000010">
        <property type="entry name" value="very large A-kinase anchor protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000011">
        <property type="entry name" value="very large A-kinase anchor protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000038">
        <property type="entry name" value="very large A-kinase anchor protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF38">
        <property type="entry name" value="VERY LARGE A-KINASE ANCHOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0430">Lectin</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="6">
        <location>
            <begin position="2009"/>
            <end position="2058"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="6">
        <location>
            <begin position="2104"/>
            <end position="2142"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="6">
        <location>
            <begin position="2154"/>
            <end position="2198"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="6">
        <location>
            <begin position="2199"/>
            <end position="2241"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="6">
        <location>
            <begin position="2287"/>
            <end position="2329"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="6">
        <location>
            <begin position="2466"/>
            <end position="2507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="611"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="744"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1557"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1761"/>
            <end position="1794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="611"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="747"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1558"/>
            <end position="1577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2643" mass="293296" checksum="B8D1B0642C09E0BE" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HJ85</accession>
    <name>A0A452HJ85_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(S)-lactoyl-CoA + L-lysyl-[protein] = N(6)-[(S)-lactoyl]-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61996"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:19466"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:231527"/>
            <dbReference type="ChEBI" id="CHEBI:231528"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:61997"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014975"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017106.1">
        <property type="protein sequence ID" value="ENSGAGP00000014975.1"/>
        <property type="gene ID" value="ENSGAGG00000011318.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05495">
        <property type="entry name" value="Bromo_cbp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20910">
        <property type="entry name" value="NCBD_CREBBP-p300_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15646">
        <property type="entry name" value="PHD_p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15802">
        <property type="entry name" value="RING_CBP-p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02337">
        <property type="entry name" value="ZZ_CBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.20:FF:000001">
        <property type="entry name" value="E1A binding protein p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1020.10:FF:000001">
        <property type="entry name" value="E1A binding protein p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1020.10:FF:000002">
        <property type="entry name" value="E1A binding protein p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.40:FF:000001">
        <property type="entry name" value="E1A binding protein p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000003">
        <property type="entry name" value="E1A binding protein p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000001">
        <property type="entry name" value="Histone acetyltransferase p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000034">
        <property type="entry name" value="Histone acetyltransferase p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1630.10">
        <property type="entry name" value="Nuclear receptor coactivator, CREB-bp-like, interlocking domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1020.10">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031162">
        <property type="entry name" value="CBP_P300_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003101">
        <property type="entry name" value="KIX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009110">
        <property type="entry name" value="Nuc_rcpt_coact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014744">
        <property type="entry name" value="Nuc_rcpt_coact_CREBbp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037073">
        <property type="entry name" value="Nuc_rcpt_coact_CREBbp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056484">
        <property type="entry name" value="PHD_P300"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010303">
        <property type="entry name" value="RING_CBP-p300"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038547">
        <property type="entry name" value="RING_CBP-p300_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035898">
        <property type="entry name" value="TAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000197">
        <property type="entry name" value="Znf_TAZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808">
        <property type="entry name" value="CBP/P300-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808:SF29">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE P300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09030">
        <property type="entry name" value="Creb_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08214">
        <property type="entry name" value="HAT_KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02172">
        <property type="entry name" value="KIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23570">
        <property type="entry name" value="PHD_P300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06001">
        <property type="entry name" value="RING_CBP-p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02135">
        <property type="entry name" value="zf-TAZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01250">
        <property type="entry name" value="KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00551">
        <property type="entry name" value="ZnF_TAZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47040">
        <property type="entry name" value="Kix domain of CBP (creb binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69125">
        <property type="entry name" value="Nuclear receptor coactivator interlocking domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57933">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51727">
        <property type="entry name" value="CBP_P300_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50952">
        <property type="entry name" value="KIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50134">
        <property type="entry name" value="ZF_TAZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="20" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="16" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="17 22" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="18" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12 23" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 24" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TAZ-type" evidence="27">
        <location>
            <begin position="339"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="KIX" evidence="29">
        <location>
            <begin position="571"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="26">
        <location>
            <begin position="1092"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="domain" description="CBP/p300-type HAT" evidence="30">
        <location>
            <begin position="1312"/>
            <end position="1688"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="28">
        <location>
            <begin position="1690"/>
            <end position="1738"/>
        </location>
    </feature>
    <feature type="domain" description="TAZ-type" evidence="27">
        <location>
            <begin position="1753"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TAZ-type" evidence="23">
        <location>
            <begin position="339"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TAZ-type" evidence="23">
        <location>
            <begin position="1753"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="99"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="256"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="662"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="715"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="759"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1030"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1545"/>
            <end position="1603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1858"/>
            <end position="1955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="2023"/>
            <end position="2049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="2129"/>
            <end position="2202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="2224"/>
            <end position="2459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="281"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="488"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="715"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="760"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="784"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="25">
        <location>
            <begin position="844"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="857"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="25">
        <location>
            <begin position="880"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="891"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="25">
        <location>
            <begin position="903"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="921"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="950"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="980"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1030"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="1057"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1545"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="1573"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="25">
        <location>
            <begin position="1874"/>
            <end position="1918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="25">
        <location>
            <begin position="1939"/>
            <end position="1955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="2029"/>
            <end position="2045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="2129"/>
            <end position="2155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="2177"/>
            <end position="2200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="2224"/>
            <end position="2235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="2250"/>
            <end position="2288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="2301"/>
            <end position="2313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="2324"/>
            <end position="2350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="25">
        <location>
            <begin position="2356"/>
            <end position="2381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="2382"/>
            <end position="2394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="2412"/>
            <end position="2422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="2438"/>
            <end position="2459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="2459" mass="269473" checksum="741007151F8A0E50" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HL10</accession>
    <name>A0A452HL10_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HL10"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000015620"/>
    <dbReference type="Ensembl" id="ENSGAGT00000017840.1">
        <property type="protein sequence ID" value="ENSGAGP00000015620.1"/>
        <property type="gene ID" value="ENSGAGG00000011779.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF45">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018973398" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="4"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000015620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="163" mass="18501" checksum="AAD2867961E6F5E7" modified="2019-05-08" version="1">MKAPDILLWTLTAFLSLGFSQGQGSVTQTQGQLEKLKGQTVTLECTFSTGYQYYYLFWYRQQRSGTMDFLFRISQSNAEKNGAGRRFSAEFRKSSNFCSLTIKELEPTDSAMYFCALMEHIVRSLIGSPVQKPTPLTFSSPLTTAEFNPEDTDRWLNQMVGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HMT9</accession>
    <name>A0A452HMT9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HMT9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016277"/>
    <dbReference type="Ensembl" id="ENSGAGT00000018581.1">
        <property type="protein sequence ID" value="ENSGAGP00000016277.1"/>
        <property type="gene ID" value="ENSGAGG00000012206.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001072">
        <property type="entry name" value="Immunoglobulin heavy variable V1-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="11" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="14"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="144" mass="16645" checksum="EF103DCCD937FC5B" modified="2019-05-08" version="1">IQFIQSGAEIKKPGESVKLTCKASGYTFTDYSISWVRQAPGKGLEWVATIRTDNFNTFYPEALKGRTTISVDTSISTTYLQMNSLKSEDTAVYYCGISPWTRRLYYLCYWHWCCSCWNAVSTSSGCRSRRMLIDWRQFREVPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HP83</accession>
    <name>A0A452HP83_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000016778"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019152.1">
        <property type="protein sequence ID" value="ENSGAGP00000016778.1"/>
        <property type="gene ID" value="ENSGAGG00000012535.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000019160.1">
        <property type="protein sequence ID" value="ENSGAGP00000016786.1"/>
        <property type="gene ID" value="ENSGAGG00000012535.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000019166.1">
        <property type="protein sequence ID" value="ENSGAGP00000016790.1"/>
        <property type="gene ID" value="ENSGAGG00000012535.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000019172.1">
        <property type="protein sequence ID" value="ENSGAGP00000016796.1"/>
        <property type="gene ID" value="ENSGAGG00000012535.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14037">
        <property type="entry name" value="STKc_NAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000072">
        <property type="entry name" value="AP2 associated kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051744">
        <property type="entry name" value="AP2_assoc_SerThr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028182">
        <property type="entry name" value="BMP2K_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47907:SF5">
        <property type="entry name" value="AP2 ASSOCIATED KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47907">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15282">
        <property type="entry name" value="BMP2K_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="46"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="337"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="549"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="637"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="951"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1264"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="425"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="549"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="570"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="646"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="968"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000016786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1285" mass="139839" checksum="61921FA35CDFBEDD" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HQE9</accession>
    <name>A0A452HQE9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">hypoxia-inducible factor-proline dioxygenase</fullName>
            <ecNumber evidence="7">1.14.11.29</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-prolyl-[hypoxia-inducible factor alpha subunit] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[hypoxia-inducible factor alpha subunit] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:48400"/>
            <dbReference type="Rhea" id="RHEA-COMP:12093"/>
            <dbReference type="Rhea" id="RHEA-COMP:12094"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.14.11.29" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452HQE9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017253"/>
    <dbReference type="Ensembl" id="ENSGAGT00000019681.1">
        <property type="protein sequence ID" value="ENSGAGP00000017253.1"/>
        <property type="gene ID" value="ENSGAGG00000012848.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160082">
        <property type="term" value="F:hypoxia-inducible factor-proline dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051559">
        <property type="entry name" value="HIF_prolyl_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12907:SF4">
        <property type="entry name" value="EGL NINE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12907">
        <property type="entry name" value="EGL NINE HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="128" mass="14595" checksum="7D012A27770578A7" modified="2019-05-08" version="1">MVACYPGNGTGYVRHVDNPNGDGRCVTCIYYLNKDWDAKVSGGILRIFPEGKAQFADIEPKFDRLLFFWSDRRNPHEVQPAFATRYAITVWYFDADERARAKVKYLTGEKGVRVELNKPSDSIGKDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HRR9</accession>
    <name>A0A452HRR9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MAPK regulated corepressor interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MCRIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HRR9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017761"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020260.1">
        <property type="protein sequence ID" value="ENSGAGP00000017761.1"/>
        <property type="gene ID" value="ENSGAGG00000013189.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029428">
        <property type="entry name" value="MCRIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14799">
        <property type="entry name" value="FAM195"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="44" mass="4872" checksum="376B1313E83F49E2" modified="2019-05-08" version="1">MTSSPVSRVVYNGKRNSSPRSPSNSSEIFTPAHEENVRFIYEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HS55</accession>
    <name>A0A452HS55_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HS55"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000017919"/>
    <dbReference type="Ensembl" id="ENSGAGT00000020440.1">
        <property type="protein sequence ID" value="ENSGAGP00000017919.1"/>
        <property type="gene ID" value="ENSGAGG00000013292.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000007">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0552">Olfaction</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="57"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000017919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="322" mass="35437" checksum="1AC4827BDBE927B9" modified="2019-05-08" version="1">GLTSWLRVFSLYQAASMEEGNRSTVSEFVLLGLTKSSELQLLLFVIFLAVYGATVLGNLLIVVLVACEPQLHTPMYMLLGNLSFLDFCYSSITAPNLLAGFFLQAQIISYGVCVAQIFFFHFIGGIKIFLLTIMAYDRYVAIFHPLRYGAIMNQAVGGGLVGASWAGGFIHSIIQVSIIIQLPFCSPHELDNFYCDVPQVIKLACVDTFAVELLMVSNSGLMTLLGFLVLLVSYSVLLAKLSTCSSEGRAKALSTCASHLTVVTLIFGPCIYIYARPFRSFPMDKAVSVLYTVVTPVLNPVIYTLRNKDVQLAVRKLRKLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HTQ8</accession>
    <name>A0A452HTQ8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DnaJ homolog subfamily B member 14</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DnaJ family. DNAJB12/DNAJB14 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HTQ8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000018484"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021072.1">
        <property type="protein sequence ID" value="ENSGAGP00000018484.1"/>
        <property type="gene ID" value="ENSGAGG00000013686.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000004">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily B, member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF4">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="11">
        <location>
            <begin position="109"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="58"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000018484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="344" mass="38322" checksum="4BFD9BD9227CC468" modified="2019-05-08" version="1">MEGNRDEAEKCIEIAREALEAANRDRALRFLHKAQKLYPTETARVLLEAIMKNGSAAGSAPYCQKPANGKDQSKPSSTKDSSASAAGESGKGYTKDQVEGVLSIKKSKNYYEVLGVSKDAGEEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQYDLTGSEEQACNQPGNGRFNFHRGCEADITPEDLFNMFFGGAFPSGSVHSFSNGRAGYSHPNQHRHSGHEREEERGDGGFSMFIQLMPIIVLILVSLLSQLMVSNPPYALYPRSGTGQTIKMKTENLGVIYYVNKDFRNEYKGVSLQKVEKNVEEDYVSNIRNNCWKERQQSKFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVC8</accession>
    <name>A0A452HVC8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair and recombination protein RAD54-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HVC8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019037"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021699.1">
        <property type="protein sequence ID" value="ENSGAGP00000019037.1"/>
        <property type="gene ID" value="ENSGAGG00000014089.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045003">
        <property type="term" value="P:double-strand break repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18067">
        <property type="entry name" value="DEXHc_RAD54A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.850:FF:000002">
        <property type="entry name" value="DNA repair and recombination protein RAD54-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000010">
        <property type="entry name" value="DNA repair and recombination protein RAD54-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000332">
        <property type="entry name" value="DNA repair and recombination protein RAD54-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="200"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="529"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00015341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="774" mass="87165" checksum="CB227D8824261BB3" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVI6</accession>
    <name>A0A452HVI6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Inactive tyrosine-protein kinase PEAK1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pseudopodium-enriched atypical kinase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Sugen kinase 269</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Tyrosine-protein kinase SgK269</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Probable catalytically inactive kinase. Scaffolding protein that regulates the cytoskeleton to control cell spreading and migration by modulating focal adhesion dynamics. Acts as a scaffold for mediating EGFR signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000019156"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021828.1">
        <property type="protein sequence ID" value="ENSGAGP00000019156.1"/>
        <property type="gene ID" value="ENSGAGG00000014165.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000437">
        <property type="entry name" value="Pseudopodium enriched atypical kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051511">
        <property type="entry name" value="MitoQC_Scaffold_Kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22972:SF5">
        <property type="entry name" value="INACTIVE TYROSINE-PROTEIN KINASE PEAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22972">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="1324"/>
            <end position="1684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="28"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="338"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="488"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="557"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="677"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="719"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="777"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="962"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1002"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1086"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1221"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1421"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="338"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="356"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="499"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="572"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="680"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="719"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="754"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="828"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="848"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1097"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1228"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1423"/>
            <end position="1435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1755" mass="194859" checksum="CD614D0BF72A3910" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HVS3</accession>
    <name>A0A452HVS3_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HVS3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019244"/>
    <dbReference type="Ensembl" id="ENSGAGT00000021922.1">
        <property type="protein sequence ID" value="ENSGAGP00000019244.1"/>
        <property type="gene ID" value="ENSGAGG00000014222.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
        <property type="term" value="C:dystrophin-associated glycoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030429">
        <property type="entry name" value="Sarcospan"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15260">
        <property type="entry name" value="SARCOSPAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15260:SF1">
        <property type="entry name" value="SARCOSPAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="145" mass="16055" checksum="778811F426D685DE" modified="2019-05-08" version="1">MRKEEKKPQNNTSLKNQNQAGKAPEGEKSTDPAKQQEPAMKKKAKGDPKTGQEEESHTCCGCRFPLLVALLQLVLGISITVLGFIMAGISSSLLVRDTPYWAGIIVCVVALVGMVMLCVSYQPEEKTCFQFAIKVQYNFFKSCHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWG3</accession>
    <name>A0A452HWG3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="38772.ENSGAGP00000019511"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022214.1">
        <property type="protein sequence ID" value="ENSGAGP00000019511.1"/>
        <property type="gene ID" value="ENSGAGG00000014383.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595:SF1">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="13"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="251" mass="28404" checksum="E6E43D851C68530C" modified="2019-05-08" version="1">QVTMPPRAKRAPAWSNGELMDLISVWGEEAVQSQLRSTRRNYNTYGQISRAMLETGHDRDAVQCRVKVKELRSAYCKACEGNRRSVAAPTTCCFYKELDAILGGDPTANPMTTMDSSERGEEGRWGETESEXTCPLNALGIFKIPFLFPQPGVERNQSXSHSSWNLLVKDKQRSGFPVSSFYFQDGNGSGKEGGLRLKEDMVKAVMNQTARESKKLQDWREKMQHWREGESRRKALAKKKSTKQLISILVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HWM0</accession>
    <name>A0A452HWM0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HWM0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000019513"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022216.1">
        <property type="protein sequence ID" value="ENSGAGP00000019513.1"/>
        <property type="gene ID" value="ENSGAGG00000014384.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000019513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="190" mass="20909" checksum="4EDDF77B5286AAFC" modified="2019-05-08" version="1">MSSMEKHLFNLKFAAKELSRNAKKCDKEEKIEKTKIKKAIQKGNTEVARIHAENAIRQKNQAINFLRMSARVDAVAARVQTAVTMGKVTKSMAGVVKSMDATLKSMNLEKISALMDKFEHQFETLDVQTAQMEDTMSNTTTLTTPQNQVDTLLQEMADEAGLDLNMELPQGQTGSVGTSVASAEQVNTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HXZ9</accession>
    <name>A0A452HXZ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hydroxysteroid dehydrogenase-like protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HXZ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020075"/>
    <dbReference type="Ensembl" id="ENSGAGT00000022868.1">
        <property type="protein sequence ID" value="ENSGAGP00000020075.1"/>
        <property type="gene ID" value="ENSGAGG00000014801.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09762">
        <property type="entry name" value="HSDL2_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000301">
        <property type="entry name" value="Hydroxysteroid dehydrogenase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051935">
        <property type="entry name" value="HSDL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006133">
        <property type="entry name" value="PRK08278.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808:SF3">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="10">
        <location>
            <begin position="350"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="294"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="295"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="446" mass="47566" checksum="8E99AF3E6251EDB1" modified="2019-05-08" version="1">MLPNTGKLAGCTLFITGASRGIGKAIALKAAKDGANIVIAAKTGEPHRTLPGTIYTAAEEIEAAGGKALPCIVNVREEQQIADAVEKAVQKFGGIDILVNNASAISLTGTLETPTKKVDLMMSANTRGTYLASKICLPYLRKSNIAHILNISPPINLNPIWFKNHCAYTISKYGMSMCVLGMAEEFRGEVAVNALWPQTAIHTSAMDMLGGAGVEQQCRKTDILADAAYCILTKPKSFTGNFVIDENLLREEGIKNFDVYAVAPGHPLLPDFFLDVDPDTLAMKMEAHGASPVFREGKKIESAKPEGPDGAKPEGHDGTKSEGLDGAESAAVKPLGPVEETFKIIKGAISEDVVRSTQGVYLFELSGDEGGTWYIDLKNKAGSAGIGEPPVKVDVVMSMSSGDFVKMFSGKLKPTMAFMSGKLKIKGNMALAMKLEKMMTQFSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0D1</accession>
    <name>A0A452I0D1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc finger protein RFP-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I0D1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000020915"/>
    <dbReference type="Ensembl" id="ENSGAGT00000023821.1">
        <property type="protein sequence ID" value="ENSGAGP00000020915.1"/>
        <property type="gene ID" value="ENSGAGG00000015363.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19762">
        <property type="entry name" value="Bbox2_TRIM7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16594">
        <property type="entry name" value="RING-HC_TRIM7-like_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12888">
        <property type="entry name" value="SPRY_PRY_TRIM7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="90"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="209"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="185"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000020915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="45007" checksum="C4E0620C569F31C7" modified="2019-05-08" version="1">KAAALLVGSNCEEATCPVCLEYFTEPVTLDCGHNFCRACIAQCWEGSDTAASCPQCRETVQQRNLRPNRQLANVIEIAKQLSLQAAKGTGEDGVCGKHQEALKLFCEEDQTPICVICRESRAHRTHTVVPIQEAAQEHKERIQAHLKTLREEREKLLGLKVTGERNSREYLVGRQKIVSEFQQLRQFLEEQERLLLAQLEKLDEEIVRIQNENVSKLSEQISHLSELISELEGKCQKPASEFLQVRLNEKHSLGGTQQNLPNNPERFDYWSCMLGCERFTSGRHCWEVEVRDQGGWAVGVARESSVRRKGQISHSPEGGIWAVGRWLGQFQALTSPETLLPLSRVPSRTRVCLDCDRGQVTFIDAGDEAPIFTFPPGSVPGERIRPWLWVGGSRLRLCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I0S8</accession>
    <name>A0A452I0S8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to extracellular matrix proteins. Binds to pathogen-associated molecular patterns (PAMPs) present on the cell walls of Gram-positive and Gram-negative bacteria and fungi, behaving as a pattern recognition receptor (PRR). Induces bacterial and fungal aggregation and subsequent inhibition of PAMP-induced cytokine release. Does not possess intrinsic bactericidal activity. May play a role in the innate defense and homeostasis of certain epithelial surfaces.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with LGALS1 and laminin.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I0S8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021108"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024045.1">
        <property type="protein sequence ID" value="ENSGAGP00000021108.1"/>
        <property type="gene ID" value="ENSGAGG00000015505.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000007">
        <property type="entry name" value="Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF487">
        <property type="entry name" value="SOLUBLE SCAVENGER RECEPTOR CYSTEINE-RICH DOMAIN-CONTAINING PROTEIN SSC5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="25"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="50"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="63"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="139" mass="15415" checksum="63FCDE23D2D7961A" modified="2019-05-08" version="1">MHRNLPECFFPWVFADADISEVAQLRLVNSRSHCAGRVEMLHNRQWGTVCDDSWDLQDARVVCRQLGCGTALSAPGGARFGRGSDRIWLDNVNCTGTEAALSDCRTRPWGENNCNHGEDAGVVCSGNLHPSRHCGWCRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I188</accession>
    <name>A0A452I188_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FAM184A/B N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I188"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021253"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024204.1">
        <property type="protein sequence ID" value="ENSGAGP00000021253.1"/>
        <property type="gene ID" value="ENSGAGG00000015606.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039478">
        <property type="entry name" value="FAM184A/B_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18870:SF7">
        <property type="entry name" value="PROTEIN FAM184A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18870">
        <property type="entry name" value="PROTEIN TAG-278-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15665">
        <property type="entry name" value="FAM184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein FAM184A/B N-terminal" evidence="4">
        <location>
            <begin position="56"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1082"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="57"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="222"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="297"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="500"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="717"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="975"/>
            <end position="1002"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1151" mass="134496" checksum="C16858089279D4EA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I1J6</accession>
    <name>A0A452I1J6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="8">PPIase</shortName>
            <ecNumber evidence="8">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the activated spliceosome B/catalytic step 1 spliceosome, one of the forms of the spliceosome which has a well-formed active site but still cannot catalyze the branching reaction and is composed at least of 52 proteins, the U2, U5 and U6 snRNAs and the pre-mRNA. Recruited during early steps of activated spliceosome B maturation, it is probably one of the first proteins released from this complex as he matures to the spliceosome C complex. Component of the minor spliceosome, which splices U12-type introns.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A452I1J6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021336"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024302.1">
        <property type="protein sequence ID" value="ENSGAGP00000021336.1"/>
        <property type="gene ID" value="ENSGAGG00000015669.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01925">
        <property type="entry name" value="cyclophilin_CeCYP16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000007">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase CWC27 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF6">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="9">
        <location>
            <begin position="19"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="171" mass="19213" checksum="E8D7C87D8A8E335D" modified="2019-05-08" version="1">MSNIYIQEPPTNGKVLLKTTAGDIDIELWSKEAPKACRNFIQLCMEGYYDNTIFHRVVPDFIVQGGDPTGTGSGGESIYGLPFKDEFHSRLRFNRRGLVAMANAGVHDNGSQFFFTLSRADELNNKHTIFGKITGDTIYNMLRLTEVEIDKEERPLNPHKIKSSEVCIAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I206</accession>
    <name>A0A452I206_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Oligomeric complex of 4 set of homotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SFTPD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I206"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000021526"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024515.1">
        <property type="protein sequence ID" value="ENSGAGP00000021526.1"/>
        <property type="gene ID" value="ENSGAGG00000015805.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000045">
        <property type="entry name" value="Pulmonary surfactant-associated protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.360">
        <property type="entry name" value="SFTPD helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051077">
        <property type="entry name" value="Ca-dependent_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015097">
        <property type="entry name" value="Surfac_D-trimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024:SF15">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09006">
        <property type="entry name" value="Surfac_D-trimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57944">
        <property type="entry name" value="Triple coiled coil domain of C-type lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019225991" description="C-type lectin domain-containing protein" evidence="12">
        <location>
            <begin position="22"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="13">
        <location>
            <begin position="149"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="44"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="251" mass="26812" checksum="4961EDB470187569" modified="2019-05-08" version="1">MHFHAFHILLLGMSLVREYNSSQCTCEEKANTCTFIPCGIPGSNGLPGRDGKEGPKGEKGELGLRGTQGLPGKAGPSGPKGEKGSVGERGPTGPSGGQELDHLKTQLRELQAQLKNLQATFSKAQKVLLFPNGMTVGEKIFKTEGSEGNFETSKAACSQAGGLLASPRNSAENSAIQQIAARHKKMPFLGINDIQTEGTFKHLNGEALGYSNWASNEPNNAEGIEDCVEVYSSGKWNDKSCGEKRLIICEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I274</accession>
    <name>A0A452I274_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I274"/>
    <dbReference type="Ensembl" id="ENSGAGT00000024588.1">
        <property type="protein sequence ID" value="ENSGAGP00000021588.1"/>
        <property type="gene ID" value="ENSGAGG00000015841.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030550">
        <property type="term" value="F:acetylcholine receptor inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23543">
        <property type="entry name" value="TFP_LU_ECD_Ly6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000003">
        <property type="entry name" value="lymphocyte antigen 6E isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051110">
        <property type="entry name" value="Ly-6/neurotoxin-like_GPI-ap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035076">
        <property type="entry name" value="Toxin/TOLIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16983:SF13">
        <property type="entry name" value="LYMPHOCYTE ANTIGEN 6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16983">
        <property type="entry name" value="UPAR/LY6 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00087">
        <property type="entry name" value="Toxin_TOLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00134">
        <property type="entry name" value="LU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019118075" description="UPAR/Ly6 domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="7">
        <location>
            <begin position="21"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000021588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="126" mass="13557" checksum="30E2221A2EF5EC2F" modified="2019-05-08" version="1">MKAFLFALLAAVLCAERVSSLKCFTCNDEPSNWNCIKITDCAENDKYCLTTYTKTGLGEKAEYRITKKCSAECPQTNWNVGIAAASTSCCQHSLCNISGASSVKSSYAVMATGILASLIYVLRSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3P8</accession>
    <name>A0A452I3P8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nitric oxide synthase 1 adaptor protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452I3P8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022141"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025237.1">
        <property type="protein sequence ID" value="ENSGAGP00000022141.1"/>
        <property type="gene ID" value="ENSGAGG00000016259.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027851">
        <property type="entry name" value="DUF4628"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15429">
        <property type="entry name" value="DUF4628"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="30523" checksum="2816580BF098F636" modified="2019-05-08" version="1">PCLFLILLIPREQASSTIDQSMFENSNTAPTPKLQHSLSVSKASLGPPPAALPAAAETGLRVGSSQHLKNLSKAVGAKVNDFLRRKEAASLGEVGVTEINASAGAKLSGGMLPWLGDAARVLLETFPRLDPPPPAAKKRTPRALKTPLDMLISSQPVVSSLECSAEALPEQGQELLKQAAQGEQWKVAWDMSQPEGSAELGAPGTAVANGDGPSHSRVLSVPDLIHEENLEGKFKAADRRAASPPCPEKPTLRLSCLLEYQPPVGNIQPVAQPSSLENEGPHPDLLSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I3R4</accession>
    <name>A0A452I3R4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FACT complex subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the FACT complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase M24 family. SPT16 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I3R4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022157"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025256.1">
        <property type="protein sequence ID" value="ENSGAGP00000022157.1"/>
        <property type="gene ID" value="ENSGAGG00000016276.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032786">
        <property type="term" value="P:positive regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01091">
        <property type="entry name" value="CDC68-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000003">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.210:FF:000001">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000017">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000005">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000005">
        <property type="entry name" value="SPT16 homolog, facilitates chromatin-remodeling subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.210">
        <property type="entry name" value="FACT complex subunit Spt16p/Cdc68p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029148">
        <property type="entry name" value="FACT-SPT16_Nlobe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056595">
        <property type="entry name" value="Fact-SPT16_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048969">
        <property type="entry name" value="FACT_SPT16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013953">
        <property type="entry name" value="FACT_SPT16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040258">
        <property type="entry name" value="Spt16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033825">
        <property type="entry name" value="Spt16_M24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980">
        <property type="entry name" value="CDC68 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980:SF15">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14826">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24824">
        <property type="entry name" value="PH_SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08644">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21091">
        <property type="entry name" value="SPT16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01285">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01286">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FACT complex subunit SPT16 N-terminal lobe" evidence="12">
        <location>
            <begin position="3"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="FACT complex subunit SPT16 middle" evidence="13">
        <location>
            <begin position="519"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="14">
        <location>
            <begin position="796"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="481"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="909"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="489"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="917"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="975"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1006"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1024"/>
            <end position="1033"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1039" mass="118663" checksum="CBE1C39EC522146B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4D8</accession>
    <name>A0A452I4D8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18 22">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="18 22">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with UFD1. Interacts (via UBA domains) with SIAH2 (when ubiquitinated). Interacts with BAG6; the interaction is direct and may mediate UBL4A deubiquitination. Interacts (via UBA 2 domain) with AMFR; the interaction is direct. Interacts with UBL4A; may be indirect via BAG6. Interacts with NEDD4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18 22">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="18 22"/>
    <dbReference type="AlphaFoldDB" id="A0A452I4D8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022430"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025557.1">
        <property type="protein sequence ID" value="ENSGAGP00000022430.1"/>
        <property type="gene ID" value="ENSGAGG00000016460.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000025558.1">
        <property type="protein sequence ID" value="ENSGAGP00000022431.1"/>
        <property type="gene ID" value="ENSGAGG00000016460.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02658">
        <property type="entry name" value="Peptidase_C19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14384">
        <property type="entry name" value="UBA1_UBP13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14386">
        <property type="entry name" value="UBA2_UBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000016">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000047">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000026">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000770">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000063">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016652">
        <property type="entry name" value="Ubiquitinyl_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF105">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016308">
        <property type="entry name" value="UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 18 22" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 18 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 18 22" id="KW-0645">Protease</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 18 22" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11 18 22" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14 18 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="25">
        <location>
            <begin position="183"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="24">
        <location>
            <begin position="332"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="23">
        <location>
            <begin position="648"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="23">
        <location>
            <begin position="723"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="19">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="822"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00064879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR016308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="862" mass="96644" checksum="1BE82D43800580A1" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I4L6</accession>
    <name>A0A452I4L6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thiamine-triphosphatase</fullName>
            <ecNumber evidence="5">3.6.1.28</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Hydrolase highly specific for thiamine triphosphate (ThTP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>thiamine triphosphate + H2O = thiamine diphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11744"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:58938"/>
            <dbReference type="EC" id="3.6.1.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ThTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.28" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A452I4L6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000022458"/>
    <dbReference type="Ensembl" id="ENSGAGT00000025586.1">
        <property type="protein sequence ID" value="ENSGAGP00000022458.1"/>
        <property type="gene ID" value="ENSGAGG00000016470.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050333">
        <property type="term" value="F:thiamine triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042357">
        <property type="term" value="P:thiamine diphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07758">
        <property type="entry name" value="ThTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039582">
        <property type="entry name" value="THTPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012177">
        <property type="entry name" value="ThTPase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14586">
        <property type="entry name" value="THIAMINE-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14586:SF1">
        <property type="entry name" value="THIAMINE-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01928">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036561">
        <property type="entry name" value="ThTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01118">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTH" evidence="16">
        <location>
            <begin position="5"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR036561-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR036561-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000022458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="240" mass="25580" checksum="729F62263A411345" modified="2019-05-08" version="1">MPWGSIEVEAKFTAGPDTEARLVALGAALAGSSSFRDLYYDTPDLRLTRADHWLRLRQGAGWELKCPPEPPAGGTGGVPEAAAQEPPAVATTYQELTSPRAIVGRLCGVLGVAPAPAWDQVPGAVAGLDLQEFASFGTRRRSYRLGGLRVDLDQADFGYAVAEVEALVGAQEEVPAALEEVLQLRRALGRDGATRVPGKMSVYLQRHRPRHYEALLRAGVLAGDPEPPPHGDDTAKRGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I7X5</accession>
    <name>A0A452I7X5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDAH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I7X5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023736"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027035.1">
        <property type="protein sequence ID" value="ENSGAGP00000023736.1"/>
        <property type="gene ID" value="ENSGAGG00000017381.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016403">
        <property type="term" value="F:dimethylargininase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006525">
        <property type="term" value="P:arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000052">
        <property type="term" value="P:citrulline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045429">
        <property type="term" value="P:positive regulation of nitric oxide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.75.10.10:FF:000004">
        <property type="entry name" value="N(G),N(G)-dimethylarginine dimethylaminohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033199">
        <property type="entry name" value="DDAH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12737">
        <property type="entry name" value="DIMETHYLARGININE DIMETHYLAMINOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12737:SF17">
        <property type="entry name" value="N(G),N(G)-DIMETHYLARGININE DIMETHYLAMINOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19420">
        <property type="entry name" value="DDAH_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="78"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR633199-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR633199-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="285" mass="31215" checksum="96D9D5828E077CC1" modified="2019-05-08" version="1">MAGLSGSSSLGRCSHAVVRAPAESLSQHALRIEKSKEVDFARAERQHQLYVGVLKNKLGLQVLELPADESLPDCVFVEDVAVVCEETALITRPGAPSRRKEVDAMKRALESLNLNIVEMTDENATLDGGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVYDSLHLKSFCSMAGPNLIAIGSTEAAQKALKTMQQMSDHRYDKLTVPDDAAANCIYLNIPGKGHILLHRAPEEYPESAKVFEKLKDHMLIPIANTEMEKVDGLLTCCSVLINKTSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I835</accession>
    <name>A0A452I835_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytoskeleton-associated protein 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CKAP2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I835"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023681"/>
    <dbReference type="Ensembl" id="ENSGAGT00000026979.1">
        <property type="protein sequence ID" value="ENSGAGP00000023681.1"/>
        <property type="gene ID" value="ENSGAGG00000017344.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052855">
        <property type="entry name" value="CKAP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029197">
        <property type="entry name" value="CKAP2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47078">
        <property type="entry name" value="CYTOSKELETON-ASSOCIATED PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47078:SF1">
        <property type="entry name" value="CYTOSKELETON-ASSOCIATED PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15297">
        <property type="entry name" value="CKAP2_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytoskeleton-associated protein 2 C-terminal" evidence="7">
        <location>
            <begin position="353"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Cytoskeleton-associated protein 2 C-terminal" evidence="7">
        <location>
            <begin position="572"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="224"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="257"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="637" mass="70982" checksum="437B0CCB1E70ADF4" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452I8D9</accession>
    <name>A0A452I8D9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452I8D9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000023922"/>
    <dbReference type="Ensembl" id="ENSGAGT00000027245.1">
        <property type="protein sequence ID" value="ENSGAGP00000023922.1"/>
        <property type="gene ID" value="ENSGAGG00000017495.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22710">
        <property type="entry name" value="X-RAY RADIATION RESISTANCE ASSOCIATED PROTEIN 1 XRRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22710:SF2">
        <property type="entry name" value="X-RAY RADIATION RESISTANCE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="69"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="211"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000023922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="247" mass="28289" checksum="A1D40A3D9C626DB9" modified="2019-05-08" version="1">MPPPLPEWVRSPIGGSVFVRAQRALEDQPLLLFQVTSHVPKVPKQLLMLEAPLESSFWKLWLDTEGGKLEESEEGSHRLSLSDPLPPIRPSSAQQHQEPYDDTKMLGSHKEVPEKEKYDSKLGPLDDADDIPSSVWRHELEDRQGKRKHKSRTSGSCTYIPTKYKGYEELLRVKTDPEFIEPVGMQQNVQALRRALKQPLVYRDSKARLDSVQKPYVPRRKKVKHLGQRRETETKGSDLSKARQVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IAB9</accession>
    <name>A0A452IAB9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IAB9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000024640"/>
    <dbReference type="Ensembl" id="ENSGAGT00000028038.1">
        <property type="protein sequence ID" value="ENSGAGP00000024640.1"/>
        <property type="gene ID" value="ENSGAGG00000017984.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010312">
        <property type="term" value="P:detoxification of zinc ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006828">
        <property type="term" value="P:manganese ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820:SF3">
        <property type="entry name" value="CALCIUM_MANGANESE ANTIPORTER SLC30A10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820">
        <property type="entry name" value="FI23527P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="216"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="10">
        <location>
            <begin position="12"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="11">
        <location>
            <begin position="297"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="194"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000024640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="450" mass="48618" checksum="B6A0482B9FB02B1E" modified="2019-05-08" version="1">MGRYSGKTCRLISMLVLTSGFFVAELVSGYLGNSIALVSDSFNMLSDLISLCVGIATGRISRRARRSPGATYGYGRAEAVGALSNAVFLTALCFTILVEALLRLARPERIDGPELVLIVGALGLAVNLVGLLVFQDWGSCSGRLQPRAAAPEQLQAVPGPGLRAKIGVFFSVCRIYTQHRVSSLNAGDSLNTQKRPEEQTVQKKEKKSEALNIRGVLLHVMGDALGSVIVVVAAAIFYALPLDANTPCNWQCYIDPSLTLVMVVIILSSAFPLIKETTTILLQMVPKGVNMQGLSKLTDVPGVCSLHEVHVWELAGGKNIATLHVKCQTTSDYQDASYKMREVFHEAGIHSVTIQPEYIDHKTSELICSSPCISKACASQLCCPQQEVALTHDNGCLEKNGLSPTLHKNNSLNKNAVEIPIEHTLAGAIIKNTDNCKKSDDKSYLHSTHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ICX4</accession>
    <name>A0A452ICX4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine/threonine-protein phosphatase 1 regulatory subunit 10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Component of the PNUTS-PP1 complex (also named PTW/PP1 complex), composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA (or PPP1CB or PPP1CC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ICX4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000025678"/>
    <dbReference type="Ensembl" id="ENSGAGT00000029213.1">
        <property type="protein sequence ID" value="ENSGAGP00000025678.1"/>
        <property type="gene ID" value="ENSGAGG00000018744.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072357">
        <property type="term" value="C:PTW/PP1 phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46557">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46557:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="18">
        <location>
            <begin position="73"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="17">
        <location>
            <begin position="836"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="15">
        <location>
            <begin position="836"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="147"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="240"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="302"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="496"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="625"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="386"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="500"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="655"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="703"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="738"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="789"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="813"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000025678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="870" mass="91870" checksum="9F831DC3C28ACA56" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IFL2</accession>
    <name>A0A452IFL2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin conjugating enzyme E2 F (putative)</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IFL2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000026671"/>
    <dbReference type="Ensembl" id="ENSGAGT00000030313.1">
        <property type="protein sequence ID" value="ENSGAGP00000026671.1"/>
        <property type="gene ID" value="ENSGAGG00000019422.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000026671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="9041" checksum="E4FE4A6931616A87" modified="2019-05-08" version="1">MLTLASKLKRDDGVKGPRTSNPASDSTRRVSIRDKLLVKEVAELEVNLPCTCKVNFPDSNKLHYFQLTVTPGNVLKYFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452II11</accession>
    <name>A0A452II11_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the methyltransferase superfamily. METTL18 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452II11"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027482"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031217.1">
        <property type="protein sequence ID" value="ENSGAGP00000027482.1"/>
        <property type="gene ID" value="ENSGAGG00000019974.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF39">
        <property type="entry name" value="HISTIDINE PROTEIN METHYLTRANSFERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="11">
        <location>
            <begin position="173"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="367" mass="41185" checksum="97F8D142215EAE03" modified="2019-05-08" version="1">MAFQFNFSIEEKAENELETLGDTALQLESAQPALDPGRSTEKSKEISSEENVVHKESVCAHEATPKTTDPVANCNPDKSQVQVLPKKRADFKVAKEHNIPEDFSKVLENKVMDTVQGLYCVNISVMEMTLSDDSHEEDIVSQSISSHSDLITGIYEGGLKIWECTFDLMDYLSKAEMQFINKTVLDLGCGAGLLGILALKRKAEKVHFQDYNCTVIDEITLPNVVANWTDGGGDGRISEPPSKKHRKADFIPALMSKCRFFSGEWTEFSQLLLSGNKPFSKYDLILTSETIYNPDYYSALHDMISKLLDKNGCVYLATKAHYFGVGGGVHIFEKFIEERNVFRTRTVKVIDEGLMRYIIEMAFKISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452II14</accession>
    <name>A0A452II14_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein FMC1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in the assembly/stability of the mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452II14"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027487"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031223.1">
        <property type="protein sequence ID" value="ENSGAGP00000027487.1"/>
        <property type="gene ID" value="ENSGAGG00000019978.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20271">
        <property type="entry name" value="Complex1_LYR_FMC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037667">
        <property type="entry name" value="FMC1_homologue"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31716">
        <property type="entry name" value="PROTEIN FMC1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31716:SF1">
        <property type="entry name" value="PROTEIN FMC1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00045742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="113" mass="12665" checksum="4418FD224E8380F6" modified="2019-05-08" version="1">MAALGSPLCTLRSLLRELRYVSAQAGRPYRDMAAYQHLTGAFRAHRITSEKLCRAQHELHFQAATYLCLLRSVREHLALHQEYHAKGERSTEEAAGLVGLKLPQQPGGKGWDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIN8</accession>
    <name>A0A452IIN8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 11">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 11">Cell membrane</location>
            <topology evidence="3 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the connexin family. Beta-type (group I) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IIN8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027726"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031498.1">
        <property type="protein sequence ID" value="ENSGAGP00000027726.1"/>
        <property type="gene ID" value="ENSGAGG00000020156.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002267">
        <property type="entry name" value="Connexin32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF20">
        <property type="entry name" value="GAP JUNCTION BETA-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01138">
        <property type="entry name" value="CONNEXINB1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0303">Gap junction</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="13">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="14">
        <location>
            <begin position="145"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="279" mass="31914" checksum="77DA2CD8862ACB57" modified="2019-05-08" version="1">MNWAGLYTVVSGVNRHSTAIGRIWLSVIFIFRIMVLVVAAESVWGDEKSAFTCNTQQPGCNSVCYDHFFPISHVRLWALQLILVTTPALLVAMHVAYQQHQEKKLLVLTGHGDPKHLEEVKKHKMRISGSLWWTYICSVIFRLLFEAVFMYIFYMIYPGYQMIRLVKCEAYPCPNTVDCFISRPTEKTIFTVFMLATSGICIILNMAELVYLVVRACSRRAHQDSNPPSGKSSLYGHKLSTEYKQNEINQLLTEQDSSLKDMLRRNPGLQEKSDRCSAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IIY3</accession>
    <name>A0A452IIY3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Liver-expressed antimicrobial peptide 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Has an antimicrobial activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LEAP2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IIY3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000027826"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031611.1">
        <property type="protein sequence ID" value="ENSGAGP00000027826.1"/>
        <property type="gene ID" value="ENSGAGG00000020223.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061844">
        <property type="term" value="P:antimicrobial humoral immune response mediated by antimicrobial peptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009955">
        <property type="entry name" value="LEAP-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21007">
        <property type="entry name" value="LIVER EXPRESSED ANTIMICROBIAL PEPTIDE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21007:SF1">
        <property type="entry name" value="LIVER-EXPRESSED ANTIMICROBIAL PEPTIDE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07359">
        <property type="entry name" value="LEAP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="6" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="7" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019158977" description="Liver-expressed antimicrobial peptide 2" evidence="11">
        <location>
            <begin position="23"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000027826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="77" mass="8782" checksum="9804E42C20F09E47" modified="2019-05-08" version="1">MQCLKVIAVLLLCAALLSQIHCASLHRPSSHLTRQRRMTPFWRGISLRPIGAICRDDNECVSRLCRKNHCSIRISRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IJR5</accession>
    <name>A0A452IJR5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IJR5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028121"/>
    <dbReference type="Ensembl" id="ENSGAGT00000031954.1">
        <property type="protein sequence ID" value="ENSGAGP00000028121.1"/>
        <property type="gene ID" value="ENSGAGG00000020435.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120017">
        <property type="term" value="F:ceramide transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990050">
        <property type="term" value="F:phosphatidic acid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904121">
        <property type="term" value="F:phosphatidylethanolamine transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035627">
        <property type="term" value="P:ceramide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034375">
        <property type="term" value="P:high-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00025">
        <property type="entry name" value="BPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.20.10:FF:000001">
        <property type="entry name" value="Phospholipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.10:FF:000001">
        <property type="entry name" value="phospholipid transfer protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.20.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017943">
        <property type="entry name" value="Bactericidal_perm-incr_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032942">
        <property type="entry name" value="BPI/LBP/Plunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001124">
        <property type="entry name" value="Lipid-bd_serum_glycop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017954">
        <property type="entry name" value="Lipid-bd_serum_glycop_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017942">
        <property type="entry name" value="Lipid-bd_serum_glycop_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10504">
        <property type="entry name" value="BACTERICIDAL PERMEABILITY-INCREASING BPI PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10504:SF16">
        <property type="entry name" value="PHOSPHOLIPID TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01273">
        <property type="entry name" value="LBP_BPI_CETP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02886">
        <property type="entry name" value="LBP_BPI_CETP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00328">
        <property type="entry name" value="BPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00329">
        <property type="entry name" value="BPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55394">
        <property type="entry name" value="Bactericidal permeability-increasing protein, BPI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00400">
        <property type="entry name" value="LBP_BPI_CETP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Lipid-binding serum glycoprotein N-terminal" evidence="8">
        <location>
            <begin position="203"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein C-terminal" evidence="9">
        <location>
            <begin position="434"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="95"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="651"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="653"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="683" mass="74188" checksum="19BC5CE6CDA67676" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IK10</accession>
    <name>A0A452IK10_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Axonemal dynein light chain domain containing 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IK10"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000028179"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032021.1">
        <property type="protein sequence ID" value="ENSGAGP00000028179.1"/>
        <property type="gene ID" value="ENSGAGG00000020476.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052845">
        <property type="entry name" value="Axonemal_dynein_LC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23052">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT CHAIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23052:SF1">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT CHAIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="277"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="49558" checksum="A14EF9B0D9DAADA7" modified="2019-05-08" version="1">MNSKPLTVSLNQMPVPGPPSASKPEGRKMKSKLPQLRNKSSMLDCSGPLPTSLQCDFIPKEILLALTSAASSAYSPEYLRPLKKNTSGSIRVPDGLWQHPIRRNKFKYLIDHPICLTGAGSKYTTLLEDSENRLRLFPSMKPSGRLEVIQLMKVMDTMLEKAGVDDEQIGITGPSQLHNVLEVLKTEQNIYNIVFHELIRQVSVDCAERGELLSKLRQRYVHLLDRIPQQMRNLYKEMMAQRVMDRHITEELYNFKESVGQLTSELCEVREHDFRVTREAERAHEELADAVRDAELNANLVEEYRELYELQRARLETQLLQLTQEKEIWSSATYDLAMKVIERNQLTLSRKLYVSEKAWTKVIKHFIVLLASQDTADLSNLQEMTEQFRELLNRIGVEVEHAENSSREKLRMVQNGLTKWMQYFQDNAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ILT2</accession>
    <name>A0A452ILT2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452ILT2"/>
    <dbReference type="Ensembl" id="ENSGAGT00000032726.1">
        <property type="protein sequence ID" value="ENSGAGP00000028808.1"/>
        <property type="gene ID" value="ENSGAGG00000020872.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000028808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="128" mass="13630" checksum="6A4EAFFA10125EC5" modified="2019-05-08" version="1">VAFSGPHITSGPGWHLSPTYTTITSTNTPDTEQMAIIKDTFMSTKGPSLPPLGLESHFTAWGLSTRDRMTVAVADSAGSPWSFTRITSLWLGPSASSKARVVLISPLYTPTRKGPAAPGSCTSYIFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452INE0</accession>
    <name>A0A452INE0_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452INE0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029380"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033361.1">
        <property type="protein sequence ID" value="ENSGAGP00000029380.1"/>
        <property type="gene ID" value="ENSGAGG00000021241.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003386">
        <property type="entry name" value="LACT/PDAT_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02450">
        <property type="entry name" value="LCAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="233" mass="26885" checksum="9C39CCF572A50398" modified="2019-05-08" version="1">MIELMYEQNGGPVVLIAHSMGNMYTLYFLNHQTQDWKDKYIKDYVSLGAPWGGVAKTLRVLASGDNNRIPVISSLKIRDQQRSAVSTNWLLPYNYTWPPDKIFVSTPTVNYTLQDYQKFYKDINFEDGWLMRQDTEPLVYQMTPPGVRLHCLFGTGVETPDSFYYEAFPDKEPKIYYSDGDGTVNLESALQCKKWVDMQKQEVILSELSGNEHIEMLSNATTISYVKKLLFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452INN3</accession>
    <name>A0A452INN3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Neuronal membrane glycoprotein M6-a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with OPRM1. Interacts with palmitoyltransferase ZDHHC17/HIP14; the interaction leads to palmitoylation of GPM6A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the myelin proteolipid protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452INN3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000029571"/>
    <dbReference type="Ensembl" id="ENSGAGT00000033580.1">
        <property type="protein sequence ID" value="ENSGAGP00000029571.1"/>
        <property type="gene ID" value="ENSGAGG00000021358.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044295">
        <property type="term" value="C:axonal growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001614">
        <property type="entry name" value="Myelin_PLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018237">
        <property type="entry name" value="Myelin_PLP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11683">
        <property type="entry name" value="MYELIN PROTEOLIPID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11683:SF4">
        <property type="entry name" value="NEURONAL MEMBRANE GLYCOPROTEIN M6-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01275">
        <property type="entry name" value="Myelin_PLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00214">
        <property type="entry name" value="MYELINPLP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00002">
        <property type="entry name" value="PLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00575">
        <property type="entry name" value="MYELIN_PLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01004">
        <property type="entry name" value="MYELIN_PLP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="213"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000029571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="278" mass="31210" checksum="1A384D903D776BF4" modified="2019-05-08" version="1">MEENMEEGQTQKGCFECCIKCLGGIPYASLIATILLYAGVALFCGCGHEALSGTVNILQTYFEMARAAGDVIDVFTMIDIFKYVIYGIAAAFFVYGILLMVEGFFTTGAIKDLYGDFKITTCGRCVSAWFIMLTYIFMLAWLGVTAFTSLPVYMYFNLWTICRNTTLVEGANLCLDLRQFGIVTIGEEKKICTMSENFLRMCESTELNMTFHLFIVALAGAGAAVIAMVHYLMVLSANWAYVKDACRMQKYEDIKSKEEQELHDIHSTRSKERLNAYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IQ66</accession>
    <name>A0A452IQ66_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Collagen VI acts as a cell-binding protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the type VI collagen family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000030255"/>
    <dbReference type="Ensembl" id="ENSGAGT00000034339.1">
        <property type="protein sequence ID" value="ENSGAGP00000030255.1"/>
        <property type="gene ID" value="ENSGAGG00000021804.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005589">
        <property type="term" value="C:collagen type VI trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01472">
        <property type="entry name" value="vWA_collagen"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd01450">
        <property type="entry name" value="vWFA_subfamily_ECM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000004">
        <property type="entry name" value="collagen alpha-6(VI) chain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000003">
        <property type="entry name" value="Collagen type VI alpha 3 chain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000016">
        <property type="entry name" value="Collagen type VI alpha 3 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000021">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF86">
        <property type="entry name" value="COLLAGEN, TYPE VI, ALPHA 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="23"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="226"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="419"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="602"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="789"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="983"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="1482"/>
            <end position="1701"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="1690"/>
            <end position="1868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000030255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1879" mass="209852" checksum="C66218D22DBA42BC" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452ITZ0</accession>
    <name>A0A452ITZ0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452ITZ0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000031572"/>
    <dbReference type="Ensembl" id="ENSGAGT00000035805.1">
        <property type="protein sequence ID" value="ENSGAGP00000031572.1"/>
        <property type="gene ID" value="ENSGAGG00000022672.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21995">
        <property type="entry name" value="HMG-box_TOX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000005">
        <property type="entry name" value="TOX high mobility group box family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051365">
        <property type="entry name" value="TOX_HMG-box_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45781">
        <property type="entry name" value="AGAP000281-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45781:SF3">
        <property type="entry name" value="TOX HIGH MOBILITY GROUP BOX FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="259"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="259"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="192"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="426"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="521"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="227"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="427"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="530"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="553"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="580" mass="63980" checksum="8F4EA3D91F314C76" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IV48</accession>
    <name>A0A452IV48_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein LBH</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LBH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IV48"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036190.1">
        <property type="protein sequence ID" value="ENSGAGP00000031911.1"/>
        <property type="gene ID" value="ENSGAGG00000022905.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013294">
        <property type="entry name" value="LBH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038990">
        <property type="entry name" value="LBH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042945">
        <property type="entry name" value="LBH_dom_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14987:SF2">
        <property type="entry name" value="PROTEIN LBH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14987">
        <property type="entry name" value="PROTEIN LBH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15317">
        <property type="entry name" value="Lbh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008130">
        <property type="entry name" value="LBH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01881">
        <property type="entry name" value="LBHPROTEIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="LBH" evidence="10">
        <location>
            <begin position="18"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="68"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF15317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000031911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="107" mass="12474" checksum="7B9F2DF5E709EF97" modified="2019-05-08" version="1">MSVFFPIHCPDYLRSAEMTEVMMSATSMDEIGLSPRKDGLSYQIFPDPSDFDRYCKLKDRLPSIVVEPTEGDVESGELRWPPEEFLVQEEEEEEENHEEAKKENKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IW90</accession>
    <name>A0A452IW90_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">c-SKI SMAD4-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IW90"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032294"/>
    <dbReference type="Ensembl" id="ENSGAGT00000036612.1">
        <property type="protein sequence ID" value="ENSGAGP00000032294.1"/>
        <property type="gene ID" value="ENSGAGG00000023112.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21080">
        <property type="entry name" value="DHD_Skor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.390.10:FF:000001">
        <property type="entry name" value="SKI family transcriptional corepressor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.20:FF:000003">
        <property type="entry name" value="SKI family transcriptional corepressor 1 homolog-B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.390.10">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.20">
        <property type="entry name" value="Ski"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014890">
        <property type="entry name" value="c-SKI_SMAD4-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010919">
        <property type="entry name" value="SAND-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003380">
        <property type="entry name" value="SKI/SNO/DAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037000">
        <property type="entry name" value="Ski_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023216">
        <property type="entry name" value="Tscrpt_reg_SKI_SnoN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10005:SF8">
        <property type="entry name" value="SKI FAMILY TRANSCRIPTIONAL COREPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10005">
        <property type="entry name" value="SKI ONCOGENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08782">
        <property type="entry name" value="c-SKI_SMAD_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02437">
        <property type="entry name" value="Ski_Sno_DHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01046">
        <property type="entry name" value="c-SKI_SMAD_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63763">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="c-SKI SMAD4-binding" evidence="9">
        <location>
            <begin position="152"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="362"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="452"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="545"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="630"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="697"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="365"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="638"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="654"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="801" mass="86851" checksum="F70C82A218E6A305" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IXE7</accession>
    <name>A0A452IXE7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452IXE7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032729"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037093.1">
        <property type="protein sequence ID" value="ENSGAGP00000032729.1"/>
        <property type="gene ID" value="ENSGAGG00000023390.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050938">
        <property type="entry name" value="Collagen_Structural_Proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456:SF3">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXV) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456">
        <property type="entry name" value="SI:CH211-266K2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="372"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="399"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="478"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="529" mass="51041" checksum="C3290BD73F799A27" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IXN1</accession>
    <name>A0A452IXN1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Replication factor A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IXN1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000032804"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037180.1">
        <property type="protein sequence ID" value="ENSGAGP00000032804.1"/>
        <property type="gene ID" value="ENSGAGG00000023442.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902230">
        <property type="term" value="P:negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043522">
        <property type="entry name" value="DDIAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35537:SF1">
        <property type="entry name" value="DNA DAMAGE-INDUCED APOPTOSIS SUPPRESSOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35537">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE APOPTOSIS SUPPRESSOR PROTEIN DDIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication factor A C-terminal" evidence="7">
        <location>
            <begin position="9"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="823"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="823"/>
            <end position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000032804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="975" mass="107928" checksum="0F380138B95DD31F" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYI9</accession>
    <name>A0A452IYI9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYI9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033161"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037585.1">
        <property type="protein sequence ID" value="ENSGAGP00000033161.1"/>
        <property type="gene ID" value="ENSGAGG00000023656.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF26">
        <property type="entry name" value="MFS-TYPE TRANSPORTER SLC18B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="177"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="398" mass="43289" checksum="681A5114FA176C17" modified="2019-05-08" version="1">CLCSVRDFSRACTVFLYKSGLEVINVIFILHQLQLVQIGAKFMFVAGMFVSGCVTILFGLLDRAPDGPVFIGLCFLVRAMDAIGFAAAVTASFSILAKAFPNNIATVMGSLEIFTGLGLVLGPPIGGFLYQSFGYEVPFIVLGCLVLILVPLNMYLLPKYDAVATKDSFWMLITLPKVVLLCFTIFSLSACLGFLDPTMSLFVSEKFKVPASYVGLVFLGLALSYTLSSPLLGFLSDKMPHLRKWLLVFGGLLTALSFFLLGPAPILHIESKLWMFVLMLVLTGFSLGMSGIPLFPEVLHCAYENGFEEGLSVLGLVSGLFSAMWSLGAFVGPTLGGYLNERLGFEWAATIQGGWVLLSGLAIGIYYILEASRRRRRTNLQTPLGTNEERAHLLASET</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYM8</accession>
    <name>A0A452IYM8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel subunit alpha-2/delta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYM8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033169"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037593.1">
        <property type="protein sequence ID" value="ENSGAGP00000033169.1"/>
        <property type="gene ID" value="ENSGAGG00000023453.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990454">
        <property type="term" value="C:L-type voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000014">
        <property type="entry name" value="voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051173">
        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013680">
        <property type="entry name" value="VDCC_a2/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013608">
        <property type="entry name" value="VWA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166:SF6">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2_DELTA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08473">
        <property type="entry name" value="VGCC_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08399">
        <property type="entry name" value="VWA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="183"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="824" mass="92851" checksum="641A9A125C8EC634" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452IYS6</accession>
    <name>A0A452IYS6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">NUBP iron-sulfur cluster assembly factor 2, cytosolic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NUBP2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1. Interacts with NUBP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452IYS6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000033219"/>
    <dbReference type="Ensembl" id="ENSGAGT00000037643.1">
        <property type="protein sequence ID" value="ENSGAGP00000033219.1"/>
        <property type="gene ID" value="ENSGAGG00000023683.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000796">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03039">
        <property type="entry name" value="NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028600">
        <property type="entry name" value="NUBP2/Cfd1_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF19">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="22"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000033219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="271" mass="28956" checksum="385127B25E2AC68F" modified="2019-05-08" version="1">MEAVGERGNLAGVGHILLVLSGKGGVGKSTISTELALALRHVGKKVGILDVDLCGPSIPQMLKVQGKGVHQCDSGWVPVFVDQDKSISLMSIGFLLEKPDDAVVWRGPKKNALIKQFISDVTWGDLDFLIVDTPPGTSDEHISTVESLHPYKPLGAVLVTTPQAVSVGDVRRELTFCKKTGLQVIGIVENMSGFVCPHCLECTNLFSKGGGEELARHAGVPFLGCVPLDPQLTQSLEEGRDFIQEFPKSSAFPALAHIAQQILDRTSPQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J1E8</accession>
    <name>A0A452J1E8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J1E8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034221"/>
    <dbReference type="Ensembl" id="ENSGAGT00000038748.1">
        <property type="protein sequence ID" value="ENSGAGP00000034221.1"/>
        <property type="gene ID" value="ENSGAGG00000024233.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF78">
        <property type="entry name" value="INTEGRIN ALPHA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0401">Integrin</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018968506" evidence="14">
        <location>
            <begin position="38"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="895"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="43"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="201"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="307"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="370"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="431"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="15">
        <location>
            <begin position="478"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="16">
        <location>
            <begin position="601"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="958" mass="106686" checksum="877FB409F09EB385" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J271</accession>
    <name>A0A452J271_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCF/LEF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J271"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034504"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039067.1">
        <property type="protein sequence ID" value="ENSGAGP00000034504.1"/>
        <property type="gene ID" value="ENSGAGG00000024527.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000039068.1">
        <property type="protein sequence ID" value="ENSGAGP00000034505.1"/>
        <property type="gene ID" value="ENSGAGG00000024527.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990907">
        <property type="term" value="C:beta-catenin-TCF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21996">
        <property type="entry name" value="HMG-box_TCF7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000001">
        <property type="entry name" value="transcription factor 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000016">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013558">
        <property type="entry name" value="CTNNB1-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024940">
        <property type="entry name" value="TCF/LEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373:SF25">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08347">
        <property type="entry name" value="CTNNB1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="HMG box" evidence="12">
        <location>
            <begin position="330"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="10">
        <location>
            <begin position="330"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="180"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="302"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="393"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="440"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="302"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="457"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="568" mass="61220" checksum="2C51B872A5B11E83" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J2Q8</accession>
    <name>A0A452J2Q8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16 17">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J2Q8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000034692"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039273.1">
        <property type="protein sequence ID" value="ENSGAGP00000034692.1"/>
        <property type="gene ID" value="ENSGAGG00000024647.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01368">
        <property type="entry name" value="KISc_KIF23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21787">
        <property type="entry name" value="RBD_KIF20A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000094">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000095">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF29">
        <property type="entry name" value="KINESIN FAMILY MEMBER 20B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 17" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13 16 17" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Kinesin motor" evidence="20">
        <location>
            <begin position="63"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="533"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="625"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="550"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="159"/>
            <end position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00055972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000034692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="898" mass="101080" checksum="F897D68FACB774B6" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J415</accession>
    <name>A0A452J415_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cell cycle checkpoint protein RAD17</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the 9-1-1 (RAD1-RAD9-HUS1) complex and RHNO1 onto chromatin, and in CHEK1 activation. Involved in homologous recombination by mediating recruitment of the MRN complex to DNA damage sites. May also serve as a sensor of DNA replication progression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Part of a DNA-binding complex containing RFC2, RFC3, RFC4 and RFC5. Interacts with RAD1 and RAD9 within the 9-1-1 (RAD1-RAD9-HUS1) complex. Interacts with RAD9B, POLE, SNU13 and MCM7. DNA damage promotes interaction with ATR or ATM and disrupts interaction with the 9-1-1 (RAD1-RAD9-HUS1) complex. Interacts (when phosphorylated) with NBN; promoting recruitment of the MRN complex to DNA damage sites.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the rad17/RAD24 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J415"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035171"/>
    <dbReference type="Ensembl" id="ENSGAGT00000039823.1">
        <property type="protein sequence ID" value="ENSGAGP00000035171.1"/>
        <property type="gene ID" value="ENSGAGG00000024996.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000714">
        <property type="entry name" value="cell cycle checkpoint protein RAD17 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004582">
        <property type="entry name" value="Checkpoint_prot_Rad17_Rad24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018324">
        <property type="entry name" value="Rad17/Rad24_fun/met"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00602">
        <property type="entry name" value="rad24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03215">
        <property type="entry name" value="Rad17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="134"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="647"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="363"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="654"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="691" mass="78223" checksum="E930699524DFED7B" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J5B9</accession>
    <name>A0A452J5B9_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J5B9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035551"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040259.1">
        <property type="protein sequence ID" value="ENSGAGP00000035551.1"/>
        <property type="gene ID" value="ENSGAGG00000025266.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007276">
        <property type="entry name" value="Nop14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183">
        <property type="entry name" value="NOP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04147">
        <property type="entry name" value="Nop14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="166"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="337"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="188"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="241"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="347"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="872" mass="100473" checksum="ACB3851D3CAD2209" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J605</accession>
    <name>A0A452J605_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452J605"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000035919"/>
    <dbReference type="Ensembl" id="ENSGAGT00000040660.1">
        <property type="protein sequence ID" value="ENSGAGP00000035919.1"/>
        <property type="gene ID" value="ENSGAGG00000025478.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05332">
        <property type="entry name" value="11beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053011">
        <property type="entry name" value="SDR_family_member_7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44269">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44269:SF1">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000035919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="335" mass="37524" checksum="E07FC9B618E9D94A" modified="2019-05-08" version="1">MEWAALLLLPLALCALLYLLGRWLLGLDGDLTLLWAEWRGKRPENELPDKVVWVTGASSGIGEELAYQLAKIGALIVLSARRENELERVKQKCLEISNLSSKDILVLPLDLTDRSSHEAATKTVLQHFGRIDILVNNGGRSQRSLFVDTKVEVYAAIMELNYLGTVSLTKHVLNHMVQREKGKIVTMSSVMGIMGAPLATGYCASKHALQGFFNSLRPELGEYPEIIITTICPGPVQSKIIHNVFTEDLSKSLENTGDQSHKMPTDRCVRLTLVSMVNDLKEAWVSDHPYLAVCYLWQYAPTWAWWLVNKMGKIRIKNFKSGVDADVSYSKKKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452J7H2</accession>
    <name>A0A452J7H2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calpain-1 catalytic subunit</fullName>
            <ecNumber evidence="6">3.4.22.52</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Calcium-activated neutral proteinase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Calpain mu-type</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Calpain-1 large subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Micromolar-calpain</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Broad endopeptidase specificity.</text>
            <dbReference type="EC" id="3.4.22.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.52" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452J7H2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000036458"/>
    <dbReference type="Ensembl" id="ENSGAGT00000041291.1">
        <property type="protein sequence ID" value="ENSGAGP00000036458.1"/>
        <property type="gene ID" value="ENSGAGG00000025858.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00214">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.380:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033883">
        <property type="entry name" value="C2_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF284">
        <property type="entry name" value="CALPAIN-1 CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="17" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 25" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 25" id="KW-0645">Protease</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="15 25" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="26">
        <location>
            <begin position="55"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="27">
        <location>
            <begin position="564"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="active site" evidence="24 25">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site" evidence="24 25">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="active site" evidence="24 25">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00031878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00032619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00054695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000036458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="677" mass="77008" checksum="1F257B69EBBBD88C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFD3</accession>
    <name>A0A452GFD3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Membrane-bound transcription factor site-1 protease</fullName>
            <ecNumber evidence="24">3.4.21.112</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Endopeptidase S1P</fullName>
        </alternativeName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>Processes precursors containing basic and hydrophobic/aliphatic residues at P4 and P2, respectively, with a relatively relaxed acceptance of amino acids at P1 and P3.</text>
            <dbReference type="EC" id="3.4.21.112"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 27">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.112" evidence="24"/>
    <dbReference type="AlphaFoldDB" id="A0A452GFD3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000253"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000289.1">
        <property type="protein sequence ID" value="ENSGAGP00000000253.1"/>
        <property type="gene ID" value="ENSGAGG00000000229.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07479">
        <property type="entry name" value="Peptidases_S8_SKI-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000008">
        <property type="entry name" value="Membrane-bound transcription factor site-1 protease preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055143">
        <property type="entry name" value="MBTP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057060">
        <property type="entry name" value="MBTPS1_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057032">
        <property type="entry name" value="MBTPS1_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034185">
        <property type="entry name" value="Site-1_peptidase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF7">
        <property type="entry name" value="MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-1 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23001">
        <property type="entry name" value="MBTP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23094">
        <property type="entry name" value="MBTPS1_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23090">
        <property type="entry name" value="MBTPS1_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 27" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="18 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 27" id="KW-0645">Protease</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 27" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="22" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="20" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="8 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="19" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="1010"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane-bound transcription factor site-1 protease-like N-terminal" evidence="31">
        <location>
            <begin position="59"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="30">
        <location>
            <begin position="217"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="MBTPS1 third" evidence="33">
        <location>
            <begin position="493"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="MBTPS1 fourth" evidence="32">
        <location>
            <begin position="625"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="1035"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="28">
        <location>
            <begin position="1035"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00050826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00066596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00067283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00081324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="Pfam" id="PF23001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF23090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="Pfam" id="PF23094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1060" mass="118933" checksum="7781717DCB4521C5" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GFV5</accession>
    <name>A0A452GFV5_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GFV5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000380"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000425.1">
        <property type="protein sequence ID" value="ENSGAGP00000000380.1"/>
        <property type="gene ID" value="ENSGAGG00000000319.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10832">
        <property type="entry name" value="PDZ_MPP6-MPP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12037">
        <property type="entry name" value="SH3_MPP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000069">
        <property type="entry name" value="MAGUK p55 subfamily member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000047">
        <property type="entry name" value="MAGUK p55 subfamily member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000146">
        <property type="entry name" value="MAGUK p55 subfamily member 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035602">
        <property type="entry name" value="MPP2_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="10">
        <location>
            <begin position="71"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="9">
        <location>
            <begin position="151"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="236"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="8">
        <location>
            <begin position="361"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="563" mass="62981" checksum="80D56B5A95C78839" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GGT4</accession>
    <name>A0A452GGT4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein 25</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts (via cytoplasmic region) with ILK.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC25 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GGT4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000775"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000873.1">
        <property type="protein sequence ID" value="ENSGAGP00000000775.1"/>
        <property type="gene ID" value="ENSGAGG00000000657.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039730">
        <property type="entry name" value="Jlp2/Ccd25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049">
        <property type="entry name" value="DUF814-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="129"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="129"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="167"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="190" mass="22353" checksum="222D4C4B1DBB2C5F" modified="2019-05-08" version="1">MGKDKYENEDLIKYGWPEDIWFHVDKLSSAHVYLRLHKGQTVDDIPKEVLIDCAHLVKANSIQGCKMNNINVVYTPWTNLKKTADMDVGQIGFHRQKDVRTVTVEKKVNEILNRLEKTKVERFPDLAAEKEARDREERNEKKAQIQEMKRKEKEEMKKKKEMEDLRSYSSLMKSENMSSNQDGNDSDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GH01</accession>
    <name>A0A452GH01_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Alpha-protein kinase 3</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in cardiomyocyte differentiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000000847"/>
    <dbReference type="Ensembl" id="ENSGAGT00000000962.1">
        <property type="protein sequence ID" value="ENSGAGP00000000847.1"/>
        <property type="gene ID" value="ENSGAGG00000000711.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055013">
        <property type="term" value="P:cardiac muscle cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16973">
        <property type="entry name" value="Alpha_kinase_ALPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000069">
        <property type="entry name" value="Alpha-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000543">
        <property type="entry name" value="Alpha-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.200.10:FF:000003">
        <property type="entry name" value="alpha-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.200.10">
        <property type="entry name" value="MHCK/EF2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47091">
        <property type="entry name" value="ALPHA-PROTEIN KINASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47091:SF1">
        <property type="entry name" value="ALPHA-PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="88"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="1288"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-type protein kinase" evidence="19">
        <location>
            <begin position="1404"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="270"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="332"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="404"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="636"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="781"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="859"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="928"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1013"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1194"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1639"/>
            <end position="1713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="279"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="357"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="649"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="659"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="711"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="886"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="955"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="974"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1702"/>
            <end position="1713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00073276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000000847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="1713" mass="187667" checksum="E053A406CC7C22E1" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GHF8</accession>
    <name>A0A452GHF8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cAMP-responsive element modulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GHF8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001054"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001186.1">
        <property type="protein sequence ID" value="ENSGAGP00000001054.1"/>
        <property type="gene ID" value="ENSGAGG00000000847.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990589">
        <property type="term" value="C:ATF4-CREB1 transcription factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003102">
        <property type="entry name" value="CREB1-like_pKID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001630">
        <property type="entry name" value="Leuzip_CREB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45879:SF4">
        <property type="entry name" value="CAMP-RESPONSIVE ELEMENT MODULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45879">
        <property type="entry name" value="CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02173">
        <property type="entry name" value="pKID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00041">
        <property type="entry name" value="LEUZIPPRCREB"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50953">
        <property type="entry name" value="KID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="KID" evidence="12">
        <location>
            <begin position="39"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="144" mass="15602" checksum="02BDCBF8540D5FB5" modified="2019-05-08" version="1">MTMEMVESQQDGSATDSVAQSDAVCIQNQPGQSQIPPIAQVTSVANAEDSADSEGVIDSQKRREILSRRPSYRKILNELSSDATGVPKIEEEKSEEEGTPSGIPAMAVPTSLYQTSTGQYSMYAKIQYSTVELSILSLSFLTFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GJ20</accession>
    <name>A0A452GJ20_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Hexosaminidase D</fullName>
            <ecNumber evidence="6">3.2.1.52</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Beta-N-acetylhexosaminidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Beta-hexosaminidase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Hexosaminidase domain-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">N-acetyl-beta-galactosaminidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Has hexosaminidase activity. Responsible for the cleavage of the monosaccharides N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) from cellular substrates. Has a preference for galactosaminide over glucosaminide substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Extracellular vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452GJ20"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000001665"/>
    <dbReference type="Ensembl" id="ENSGAGT00000001891.1">
        <property type="protein sequence ID" value="ENSGAGP00000001665.1"/>
        <property type="gene ID" value="ENSGAGG00000001333.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06565">
        <property type="entry name" value="GH20_GcnA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000068">
        <property type="entry name" value="hexosaminidase D isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038901">
        <property type="entry name" value="HEXDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21040">
        <property type="entry name" value="BCDNA.GH04120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21040:SF6">
        <property type="entry name" value="HEXOSAMINIDASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="19">
        <location>
            <begin position="66"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000001665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="478" mass="54473" checksum="1C0506BA72E7C6EB" modified="2019-05-08" version="1">MEENKMSVSSPFGLHLVHLDLKGAPPKVSYLAEIFPLFHILGANGLLIEYEDMFPYEGELKPLRAEHAYSASEIKEILNLAELHELEVIPLVQTFGHMEFVLKHQEFSHLREVEMFPNALNPHKEESLMLVSAMIDQVVALHDGLRWFHIGSDEVYYLGEGEESKQWLQKEENSIEKLCLSHMKAVASCMVSSYPEVKPIVWDDMLRGVSEETLTESGVAQLMEPMIWDYAADLDEDDKVLLIEKYRKCGFPKLWFASAFKGATGANQSLTLIGHHLKNNLQWLKVANSTPADMLQGIALTGWQRYDHFSVLCELLPVGIPSLAVCLQALQNGGYSEKVKENVEKQLGLASLEIDTFMSESSGTFPGSDIFTLVTQVCCYLKPSVDEFLERNRFVTGWFSPYHRKRRIVHPIMMHHFQPDALSLLAKWNAVMQELQAALERVFYMCTLEEWMEENVHPSFHKLQEVVDDLDKAIQAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GKA2</accession>
    <name>A0A452GKA2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GKA2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000002024"/>
    <dbReference type="Ensembl" id="ENSGAGT00000002302.1">
        <property type="protein sequence ID" value="ENSGAGP00000002024.1"/>
        <property type="gene ID" value="ENSGAGG00000001630.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001234">
        <property type="entry name" value="Zinc finger protein 394"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001437">
        <property type="entry name" value="Zinc finger protein 594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="291"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="487"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="515"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000002024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="543" mass="62545" checksum="0F092510828CFB4C" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNN5</accession>
    <name>A0A452GNN5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000003484"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004018.1">
        <property type="protein sequence ID" value="ENSGAGP00000003484.1"/>
        <property type="gene ID" value="ENSGAGG00000002812.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF57">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="272" mass="29724" checksum="067DA25067C88063" modified="2025-10-08" version="2">MXXXXXXXXXXXXPAPPVPTPGFNIYPSSSPGAGQLVQPQSPPQAYGDPAAQQPHSSITPYLPVSTGIPPGLEYLMQVSGGLNGNHNPPQRWPHLTFIGFETANKYELRDGLGRRLFEAEEQSDCCTRNCCGSLRRLRLSVAEPGGRPVLRLLRPLKCATCWCPCCLQEFSVQNAEKQTVLRVLGPCCVFRCGGDVNFEVTSDESRGVGQISKQWGGLLKEVFTDTDVFGIQFPVDLDVKMKAVLLGACFLIDYMFFEKSGTTSQRSTVLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GNP1</accession>
    <name>A0A452GNP1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GNP1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003496"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004031.1">
        <property type="protein sequence ID" value="ENSGAGP00000003496.1"/>
        <property type="gene ID" value="ENSGAGG00000002823.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070593">
        <property type="term" value="P:dendrite self-avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07693">
        <property type="entry name" value="IgC_1_Robo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000026">
        <property type="entry name" value="roundabout homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF37">
        <property type="entry name" value="ROUNDABOUT HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="12"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="112" mass="12512" checksum="36D3DAEB11D0352C" modified="2019-05-08" version="1">AGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPIPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNSSLEVASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GPC7</accession>
    <name>A0A452GPC7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GPC7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000003756"/>
    <dbReference type="Ensembl" id="ENSGAGT00000004346.1">
        <property type="protein sequence ID" value="ENSGAGP00000003756.1"/>
        <property type="gene ID" value="ENSGAGG00000003055.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019152">
        <property type="entry name" value="DUF2046"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15276:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15276">
        <property type="entry name" value="H4 D10S170 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09755">
        <property type="entry name" value="DUF2046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="384"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="414"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000003756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="461" mass="52214" checksum="70361E8135D758F6" modified="2019-05-08" version="1">MADSASESDTDGAGGSGVAPAGSAAGPGAGGGKAGGIVISPFRLEELTNRLASLQQENKVLKIELETYKLKCKALQEENRDLRKASVTIQARAEQEEEFISNTLFKKIQALQKEKETLAVNYEKEEEFLTNELSRKLMQLQHEKAELEQHLEQEQEFQVNKLMKKIKKLENDTISKQLTLEQLRREKIDLENTLEQEQEALVNRLWKRMDKLEAEKRILQEKLDQPVSAPPSPRDISMEIDSPENMMRHIRFLKNEVERLKKQLRAAQLQHSEKMAQYLEEERHMREENLRLQRKLQREMERREALCRQLSESESSLEMDDERYFNEMSAQGLRPRTVSSPIPYTPSPSSSRPISPGLSYASHTVGFTPPTSLTRAGMSYYNSPGLHVQHMGPSHGISRPSPRRSNSPDKFKRPTPPPSPNTQTPVQPPPPPPPPPVQPTVQSTASQSATFQHSVHPSSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GQZ9</accession>
    <name>A0A452GQZ9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable D-lactate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="9">1.1.2.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in D-lactate, but not L-lactate catabolic process.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A452GQZ9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004382"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005146.1">
        <property type="protein sequence ID" value="ENSGAGP00000004382.1"/>
        <property type="gene ID" value="ENSGAGG00000003639.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000030">
        <property type="entry name" value="probable D-lactate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="12">
        <location>
            <begin position="60"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="482" mass="51476" checksum="8B4ACF930AEBCA7A" modified="2019-05-08" version="1">MALQRLCRGAGALGCRRSSGLQGQMLGELVEALRAVVGSPNVSTALAVREQHGRDESMHRCSPPDAVVWPQDVEQVSKLAGICYSHGVPIIPFSTGTGLEGGVTAVRGGVCFNLTRMDRITALSTEDFTVAVEPGVTRKALNSYLRDTGLWFPVDPGADASLCGMAATSASGTNAVRYGTMRHNVLNLQVVLADGRVLHTAGRGRHFRKSAAGYNLTGLFVGSEGTLGLITQATLRLHGSPEAMVAAVCAFPSVHAAVGCTVQVLQAGIPVARIEFLDDVMMAACNRFSGLSYAVSPTLFLEFHGSPGSVEEQVRQAGEIVQLNCGSDFAWAREPEARNKLWAARHNAWYAALALRPGSKGYSTDVCVPISRLPHIVGETHRDLQESGLTGLIVGHVGDGNFHCILVFDPADLDEIQRVKEFATRLARQALALDGTCTGEHGIGLGKRHLLWEEIGELGLTIMRQIKAMLDPKNLMNPGKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GR96</accession>
    <name>A0A452GR96_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Ligand for members of the frizzled family of seven transmembrane receptors. Plays an important role in embryonic development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Secreted</location>
            <location evidence="1 12">Extracellular space</location>
            <location evidence="1 12">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GR96"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000004380"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005144.1">
        <property type="protein sequence ID" value="ENSGAGP00000004380.1"/>
        <property type="gene ID" value="ENSGAGG00000003638.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19336">
        <property type="entry name" value="Wnt_Wnt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000008">
        <property type="entry name" value="Protein Wnt-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009142">
        <property type="entry name" value="Wnt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF101">
        <property type="entry name" value="PROTEIN WNT-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01844">
        <property type="entry name" value="WNT4PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <keyword evidence="6 12" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019329342" description="Protein Wnt" evidence="13">
        <location>
            <begin position="23"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="351" mass="38959" checksum="719A4FCC7255DEDA" modified="2019-05-08" version="1">MSPQYSLRSLLLVILAAFSANASNWLYLAKLSSVGSISEEETCEKLKGLIQRQVQMCKRNLEVMDSVRRGAQLAIEECQYQFRNRRWNCSTLDTLPVFGKVVTQGTREAAFVYAISSAGVAFAVTRACSSGELEKCGCDGTVHGVSPQGFQWSGCSDNIAYGVAFSQSFVDVRERSKGASSSRALMNLHNNEAGRKAILNNMRVECKCHGVSGSCEVKTCWKAMPPFRKVGNMLKEKFDGATEVEQRKIGSTKVLVPKNSQFKPHTDEDLVYLDSSPDFCDHDLKNGVLGTSGRQCNKTSKAIDGCELMCCGRGFHTDEVEVVERCSCKFHWCCFVKCKQCHRVVEMHTCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRB2</accession>
    <name>A0A452GRB2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RCK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GRB2"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005248.1">
        <property type="protein sequence ID" value="ENSGAGP00000004477.1"/>
        <property type="gene ID" value="ENSGAGG00000003706.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005228">
        <property type="term" value="F:intracellular sodium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF35">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY T MEMBER 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="12">
        <location>
            <begin position="200"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="287" mass="31469" checksum="6C8A08DA83D1CB5A" modified="2019-05-08" version="1">MIGVCREDKNNILLNPGPCHILASSDTCFYISISKEENSAFVLRQREHLHRSRLATPAYPGLTRLPVHSIIASMGTVAMDLPDSDSSLDSADRTTLGLAADPVGTEKRHSIAPVLEVVDAIRSPTFDLLSDQSEDEATHSDDDAATSSTWVKGYPTNSPYIGSSPTLCHLVQEKLPFCCLRLDKSCHHYPYEDARAYRFQNKLIIVSAERAANGLYNFIVPLRAYYRPRKELNPIVLLLENMYGACPEHRGLGGGGGHEGTGMGWGWGQKLGGGRGGRVMQTGWRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GRV1</accession>
    <name>A0A452GRV1_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GRV1"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005523.1">
        <property type="protein sequence ID" value="ENSGAGP00000004715.1"/>
        <property type="gene ID" value="ENSGAGG00000003880.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="8"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="75" mass="8496" checksum="AB48B4A3C9ACEF70" modified="2019-05-08" version="1">VPPFARYLQGADCDGRSGQQSVSRLSPVPWIRRQPMGGPPKPRDQRTLRRLPACCPHGDRWQGPRETFPVSWPGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GS83</accession>
    <name>A0A452GS83_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Lens fiber major intrinsic protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient. Specifically expressed in lens fiber cells, this aquaporin is crucial for maintaining lens water homeostasis and transparency. Beyond water permeability, it also acts as a cell-to-cell adhesion molecule, forming thin junctions between lens fiber cells that are essential for maintaining the ordered structure and transparency of the lens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GS83"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005547.1">
        <property type="protein sequence ID" value="ENSGAGP00000004737.1"/>
        <property type="gene ID" value="ENSGAGG00000003737.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000003">
        <property type="entry name" value="Lens fiber major intrinsic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF39">
        <property type="entry name" value="LENS FIBER MAJOR INTRINSIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="242"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="275" mass="29364" checksum="66BCD412B618A47D" modified="2019-05-08" version="1">MWEVRSPSFWRAVFAEFFATMIYVFFGLGASLRWGVGPLNVLQVALAFGLAAATLVQVLGHVSGAHVNPAVTFAFLVGAQLSLLRATFYMVAQVLGGVAGAAVLYGLTPAAIRGNLALNTIHPGLTLAQATTVEIFLTLQFVLCFFATYDERHNGRVGSVALAIGFSLALGHLFGMYYTGAGMNPARSFAPAAITRNFSNHWVYWVGPILGATLAGLLYDFVLCPRMRGLAERLAILKGEQVAEGQAPPEPPEEPMELKTPPQTGPRALLALTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GSJ3</accession>
    <name>A0A452GSJ3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Family with sequence similarity 47 member E</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM47 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GSJ3"/>
    <dbReference type="Ensembl" id="ENSGAGT00000005685.1">
        <property type="protein sequence ID" value="ENSGAGP00000004863.1"/>
        <property type="gene ID" value="ENSGAGG00000003991.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032743">
        <property type="entry name" value="FAM47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46449:SF5">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 47 MEMBER E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46449">
        <property type="entry name" value="ZGC:158260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14642">
        <property type="entry name" value="FAM47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="387"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="389"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000004863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="47058" checksum="488BDFC156A4AD47" modified="2019-05-08" version="1">MPSKYFQEHNKRKFSDSLNSQRWRFLKSGLDDFRSGFPPPSDNIISCGTKGPVPIVLRNRASDASRMAQQKGRKKCTKTQVSPSKLSPLQKARRDHIAQTEHCLSQQPLALYPHLEESIPPELFKEVVGILDPEMCLNSEGQYKDCDQDSHTPQQVLYQPEDGKSKEACPRSSALKESKGKNPKYTYLSKKEVAAREKEASLSYVPPLDENVKRVTKEFCDWVDSLGGEKYKVDEATILSLFDTGYETKLVLSVPIHVVELNNVPAELRKYMGVSPPPTTVKSPLQNSCRPCLAKDPSQPKWEKIRFGAWYLEPKTWRKQRANEPLEDPNTAVDTVQNLRNQFSEKEAELMQLHGTHAFKEFLETKGYRKPEFLLQMLAAGDVNGAQERTSKGYKKESLKRPEGIREGSSSTIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GTC1</accession>
    <name>A0A452GTC1_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dUTP diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dUTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A452GTC1"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000005283"/>
    <dbReference type="Ensembl" id="ENSGAGT00000006148.1">
        <property type="protein sequence ID" value="ENSGAGP00000005283.1"/>
        <property type="gene ID" value="ENSGAGG00000004280.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241:SF0">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase-like" evidence="5">
        <location>
            <begin position="11"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000005283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="82" mass="8986" checksum="C2726F01B9E394AA" modified="2019-05-08" version="1">PFVKFARLLSPYRGTPESAEVDLYAVAPDTIDPGTQKLIPIEIWVQLPRWCYGRVAPHSGLALCAGVHVQAGAVDHDYRGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWA4</accession>
    <name>A0A452GWA4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LDL receptor related protein 1B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GWA4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006281"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007300.1">
        <property type="protein sequence ID" value="ENSGAGP00000006281.1"/>
        <property type="gene ID" value="ENSGAGG00000005052.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17073" checksum="4DF60D4DA22898D0" modified="2019-05-08" version="1">MNLCSANWSGTQCERPAPKSSKSDNISTRSIAIIVPLVLLVTLITTLVIGLLLCKRKRRTKTIRRQPMINGGINVEIGNPSYNMYELGHNHNEGGFLDPDFTVNPEKPTNYSNPVYAKLYMDGQNCRNSLASVDERKELLPKKIDIGIRETVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GWS7</accession>
    <name>A0A452GWS7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452GWS7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006576"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007650.1">
        <property type="protein sequence ID" value="ENSGAGP00000006576.1"/>
        <property type="gene ID" value="ENSGAGG00000005321.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF156">
        <property type="entry name" value="KRAB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KRAB" evidence="2">
        <location>
            <begin position="3"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="88" mass="9919" checksum="9CF6A81930CA8D60" modified="2019-05-08" version="1">MPVTFEEVAVYFTEGQGALLDPAQRALYRDVMQEIYEMVISLGFLLPKPDVIIQLERGEEPWVPDLQGSAEREMSRGTCAGGEPMKWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXC2</accession>
    <name>A0A452GXC2_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Snf2 related CREBBP activator protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXC2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006761"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007849.1">
        <property type="protein sequence ID" value="ENSGAGP00000006761.1"/>
        <property type="gene ID" value="ENSGAGG00000005393.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18003">
        <property type="entry name" value="DEXQc_SRCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000005">
        <property type="entry name" value="Photoperiod-independent early flowering 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.850:FF:000012">
        <property type="entry name" value="protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF1">
        <property type="entry name" value="HELICASE SRCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSA" evidence="11">
        <location>
            <begin position="101"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="524"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="234"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="321"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="380"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="838"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="264"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="339"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="896"/>
            <end position="907"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="907" mass="102285" checksum="915A3F948E9DF8CA" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GXN2</accession>
    <name>A0A452GXN2_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GXN2"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000006878"/>
    <dbReference type="Ensembl" id="ENSGAGT00000007978.1">
        <property type="protein sequence ID" value="ENSGAGP00000006878.1"/>
        <property type="gene ID" value="ENSGAGG00000005545.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF400">
        <property type="entry name" value="CYTOCHROME P450 2C9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000006878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="135" mass="15686" checksum="2A9EE3051C6008FF" modified="2019-05-08" version="1">WPKGYRKTHTPPHMCFLYFFPDKTDWIKFGTTIFPVLRSVLYDSKEFPNPEQFNPGHFLDENGAFKKSDFFMPFSIGKRSCLGEALARMELFLLLTTILQNFTLKSPIDPKDIDITPMSFTSNIPVSYQLIVIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZD8</accession>
    <name>A0A452GZD8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a hydroperoxide + L-cysteinyl-[protein] = S-hydroxy-L-cysteinyl-[protein] + an alcohol</text>
            <dbReference type="Rhea" id="RHEA:67124"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:17193"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:61973"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:67125"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sestrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452GZD8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000007545"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008710.1">
        <property type="protein sequence ID" value="ENSGAGP00000007545.1"/>
        <property type="gene ID" value="ENSGAGG00000006033.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070728">
        <property type="term" value="F:L-leucine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016684">
        <property type="term" value="F:oxidoreductase activity, acting on peroxide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071233">
        <property type="term" value="P:cellular response to L-leucine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990253">
        <property type="term" value="P:cellular response to leucine starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901031">
        <property type="term" value="P:regulation of response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1290.10:FF:000001">
        <property type="entry name" value="Sestrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006730">
        <property type="entry name" value="Sestrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12474">
        <property type="entry name" value="P53 REGULATED PA26 NUCLEAR PROTEIN SESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12474:SF4">
        <property type="entry name" value="SESTRIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04636">
        <property type="entry name" value="PA26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="258"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="486" mass="56278" checksum="A6B1C760C91EF0A1" modified="2019-05-08" version="1">MNRGSAGSIGSAASASEYRVCSNCRKVLRQEKRIRVSQPLTRGPSAFIPEKEVMQASGLNERTNLLVEEYSTSGRLDNITQVMSLHTQYLESFLRSQFYMLRMDGPLPLPYRHYIAIMAAARHQCSYLVNMHVDEFLKTGGIAEWLNGLEYIPQRLKNLNEINKLLAHQPWLITKEHIQKLVKTGENNWSLPELVHAVVLLAHYHALASFVFGSGINPERDPDTSNGFRLIAVNKFCVCDLANDNIENASLTSSNFGIADSLSELEALMERMKRLQEEKEEEEASQEEMATRFEKEKKESLLVVTGAFDDEMVSTSDVSRYIEDPGFGYKDFARRGEEHLPTFRAQDYTWENHGFSLVNRLYSDIGHLLDEKFRMVYNLTYNTMATHEDVDTTMLRRALFNYVHCMFGIRYDDYDYGEVNQLLERSLKVYIKTVTCYPERTTKRMYDSYWRQFKHSEKVHVNLLLMEARMQAELLYALRAITRHLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452GZQ4</accession>
    <name>A0A452GZQ4_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IAP family.</text>
    </comment>
    <dbReference type="STRING" id="38772.ENSGAGP00000007677"/>
    <dbReference type="Ensembl" id="ENSGAGT00000008861.1">
        <property type="protein sequence ID" value="ENSGAGP00000007677.1"/>
        <property type="gene ID" value="ENSGAGG00000006146.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000009">
        <property type="entry name" value="Baculoviral IAP repeat-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051190">
        <property type="entry name" value="Baculoviral_IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771:SF3">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="17" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000007677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="146" mass="16804" checksum="D247A909D83979AF" modified="2019-05-08" version="1">MKCPAAPAAALLPAEWQLYLSETRLATYRSWPFTEGCACTPQRXAEAGFIHCPSENGPDVVQCFFCFKELEGWEPEDDPIDEHRKHSSGCAFLSLRKNPVDLTLQEFLKLDKERTRNMIKKQISQKVTEFKEESERARRDIEKLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H0Q5</accession>
    <name>A0A452H0Q5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="32">NAD-dependent protein deacylase sirtuin-6</fullName>
            <ecNumber evidence="4">2.3.1.286</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="34">NAD-dependent protein deacetylase sirtuin-6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="33">Protein mono-ADP-ribosyltransferase sirtuin-6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="38 39">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="39" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="38" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="38" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>L-arginyl-[protein] + NAD(+) = N(omega)-(ADP-D-ribosyl)-L-arginyl-[protein] + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19149"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:15087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142554"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:19150"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>L-lysyl-[protein] + NAD(+) = N(6)-(ADP-D-ribosyl)-L-lysyl-[protein] + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:15088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142515"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:58221"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>N(6)-acetyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-acetyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:43636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="ChEBI" id="CHEBI:83767"/>
            <dbReference type="EC" id="2.3.1.286"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:43637"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>N(6)-hexadecanoyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-hexadecanoyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:70563"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:14175"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138936"/>
            <dbReference type="ChEBI" id="CHEBI:189673"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:70564"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>N(6)-tetradecanoyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-tetradecanoyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:70567"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:15437"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:141129"/>
            <dbReference type="ChEBI" id="CHEBI:189674"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:70568"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="31">Homodimer; binds to nucleosomes and DNA ends as a homodimer. Interacts with RELA; interferes with RELA binding to target DNA. Interacts with SMARCA5; promoting recruitment of SMARCA5/SNF2H to double-strand breaks (DSBs) sites. Interacts with the mTORC2 complex; preventing the ability of SIRT6 to deacetylate FOXO1. Interacts with the CLOCK-BMAL1 complex; recruited by the CLOCK-BMAL1 complex to regulate expression of clock-controlled genes. Interacts with CSNK2A2; preventing CSNK2A2 localization to the nucleus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="25">Belongs to the sirtuin family. Class IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452H0Q5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008021"/>
    <dbReference type="Ensembl" id="ENSGAGT00000009241.1">
        <property type="protein sequence ID" value="ENSGAGP00000008021.1"/>
        <property type="gene ID" value="ENSGAGG00000006377.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140765">
        <property type="term" value="F:histone H3K56 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046969">
        <property type="term" value="F:histone H3K9 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.200:FF:000001">
        <property type="entry name" value="NAD-dependent protein deacetylase sirtuin-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000029">
        <property type="entry name" value="NAD-dependent protein deacetylase sirtuin-6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000038">
        <property type="entry name" value="NAD-dependent protein deacetylase sirtuin-6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF12">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-0007">Acetylation</keyword>
    <keyword evidence="24" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="17" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="22" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 35" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="39" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="19" id="KW-0779">Telomere</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="15 35" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="37">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="36">
        <location>
            <begin position="260"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="36">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="36">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="35">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00038170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00050163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00050216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00051286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00066285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00071352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00076455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00083163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="333" mass="36422" checksum="65449F2A159C21D6" modified="2019-05-08" version="1">IHSVFQAQLFLVNSRWCCFSPDLSEPPQKQGMHVGSGGPNGVWTMEERGLAPKFDTTFENARPSKTHMALLELQRVGILKFLVSQNVDGLHVRSGFPRDKLAELHGNMFVEECVKCGKQYVRDAIVGSMGLKPTGRLCDASKARGLRACRGKLIDTILDWEDSLPDRDLSLADDACRKADLSVTLGTSLQIKPSGNLPLLTKRKGGKLVIVNLQATKHDRQADLRIHGYVDEVMTKLMKQLGLEIPEWTGPAVVESSELVKPEPALKLDPDAQRPAKEEPCSHHNGMAEGADGICPGLGSAREEHCDGLKQKCPGSDHSPVAAKRVRVESLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3D5</accession>
    <name>A0A452H3D5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="13">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">PPIase which accelerates the folding of proteins during protein synthesis. Has a preference for substrates containing 4-hydroxylproline modifications, including type III collagen. May also target type VI and type X collagens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer. Homodimer. Interacts with type III, type IV and type X collagens.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A452H3D5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000008927"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010257.1">
        <property type="protein sequence ID" value="ENSGAGP00000008927.1"/>
        <property type="gene ID" value="ENSGAGG00000007061.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000118">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052273">
        <property type="entry name" value="PPIase_FKBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46222:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46222">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP7/14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 13" id="KW-0697">Rotamase</keyword>
    <keyword evidence="3 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019574114" description="peptidylprolyl isomerase" evidence="14">
        <location>
            <begin position="20"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="15">
        <location>
            <begin position="45"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="179"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000008927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="211" mass="24270" checksum="24FD1F1B574AA0A5" modified="2019-05-08" version="1">MRVAFWAPLFCVVVTCVTGALIPEAEVKIEVLQKPFICQRKTKWGDMMLVHYEGYLEKDGSVFHSTHKHNNGQPMWFTLGIKEAIKGWDKGLKDMCVGEKRQLTIPPSLAYGKEGKGKIPPESTLIFNIDLLEIRSGPRSHESFQQMDLNDDWKLSKDEVKAYLKKEFEKHGASVNDSYHDVLADDIFDKEDEDRDGFISAREFTYKHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H3W8</accession>
    <name>A0A452H3W8_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H3W8"/>
    <dbReference type="Ensembl" id="ENSGAGT00000010560.1">
        <property type="protein sequence ID" value="ENSGAGP00000009196.1"/>
        <property type="gene ID" value="ENSGAGG00000007264.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000009196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="117" mass="13068" checksum="DD2913F7730A7B70" modified="2019-05-08" version="1">MHRYGQRCPPSVPLQRGNRSPHPYIGGWLRPEGRSIPQWRYHEEGGHRGARATQYAALRQGTALPLSACRTARAGCPICSRLAPIALPEPAGRIHRAMGPCQDWQVDYIGPLPPERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6J4</accession>
    <name>A0A452H6J4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Probable G-protein coupled receptor 33</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Orphan receptor; could be a chemoattractant receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the chemokine-like receptor (CMKLR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H6J4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010172"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011671.1">
        <property type="protein sequence ID" value="ENSGAGP00000010172.1"/>
        <property type="gene ID" value="ENSGAGG00000007975.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004875">
        <property type="term" value="F:complement receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000826">
        <property type="entry name" value="Formyl_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225">
        <property type="entry name" value="CHEMOTACTIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225:SF5">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00526">
        <property type="entry name" value="FMETLEUPHER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="44"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="246" mass="28017" checksum="C449ECA3F8CF29B5" modified="2019-05-08" version="1">MDQGNTTLPSTTQVSSSQPPAAVNTAHLASAVLLFPTFLVSVVGNGLYLWVLGLKMRRTVTTLWFLSLISCNLLFTLMTPFFIVYLLMDLHWVFGTAMCKLLYTFISVDMFTSAFLLTLLSLDRYILTHHPIWSRNHRTLPRAGKLIVGLWLASFGLSTPYLAFRETREVDGGRIICISNYTLSRDWNGSEMRELGRQVHQAFFMVEFLLGFLLPFCTIAGCYVRHELLWVNTRFVKGSVYCYSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H6U4</accession>
    <name>A0A452H6U4_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Amine oxidase</fullName>
            <ecNumber evidence="12">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="12">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="11">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="11 12">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A452H6U4"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010409"/>
    <dbReference type="Ensembl" id="ENSGAGT00000011948.1">
        <property type="protein sequence ID" value="ENSGAGP00000010409.1"/>
        <property type="gene ID" value="ENSGAGG00000008151.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.20:FF:000002">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000001">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000003">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049948">
        <property type="entry name" value="Cu_Am_ox_TPQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015800">
        <property type="entry name" value="Cu_amine_oxidase_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015802">
        <property type="entry name" value="Cu_amine_oxidase_N3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF4">
        <property type="entry name" value="RETINA-SPECIFIC COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02727">
        <property type="entry name" value="Cu_amine_oxidN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02728">
        <property type="entry name" value="Cu_amine_oxidN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00766">
        <property type="entry name" value="CUDAOXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01164">
        <property type="entry name" value="COPPER_AMINE_OXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 12" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 12" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase N2-terminal" evidence="14">
        <location>
            <begin position="60"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N3-terminal" evidence="15">
        <location>
            <begin position="162"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="13">
        <location>
            <begin position="306"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="10">
        <location>
            <position position="462"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="11">
        <location>
            <position position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="757" mass="85536" checksum="64CAEBAEBAE08E95" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7K6</accession>
    <name>A0A452H7K6_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein ABHD15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alpha/beta hydrolase domain-containing protein 15</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May regulate adipocyte lipolysis and liver lipid accumulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with PDE3B; this interaction regulates PDE3B's stability and expression and, thereby, impacts the antilipolytic action of insulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H7K6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010687"/>
    <dbReference type="ESTHER" id="9saur-a0a452h7k6">
        <property type="family name" value="abh_upf0017"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000012256.1">
        <property type="protein sequence ID" value="ENSGAGP00000010687.1"/>
        <property type="gene ID" value="ENSGAGG00000008340.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034338">
        <property type="term" value="F:short-chain carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000103">
        <property type="entry name" value="Abhydrolase domain-containing 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF97">
        <property type="entry name" value="ABHYDROLASE DOMAIN CONTAINING 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019091496" description="Protein ABHD15" evidence="11">
        <location>
            <begin position="29"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="425" mass="47491" checksum="6B27C53C49F7FC07" modified="2019-05-08" version="1">MLPWAGVLGFLLLLILLRVWVWVPSSSGAQLVCKPSALAAYLESQCQALKGLCEASWPWRALPSLQTLASLMGPLDSRVHFIRTYLQLSDEGLVALDWAVGPAQQERRQTSSTCSALVLLLVPNSFGKVTRNISKLCHVALLHGYRPVVFNRRGQNGCPLSTTTLQPYGDPADLREAIQYICYRCPGALLFAVGESTGAGLLFSYLGECGSSSHLTAAGCISPIFHPRGWFEAGCPWLWQQLLLMSQKVLLSRFATVLGEVLPLDRFFEGQSLRELEEVLFCQSQTWDLYWERNDSLRDVDEVAVPVLCICSQDDPVCGAPRDTLPFELFETNPYFFLVLTQGGGHCGFFKDSLCGAFWSHEVLLQFFHTIVDFLLTEDKLRGQAKKRGARPVLKVTQGRSGCRQESCCPHNSPDVYRWQRSYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H7V6</accession>
    <name>A0A452H7V6_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C69 family. Secernin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452H7V6"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000010840"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012417.1">
        <property type="protein sequence ID" value="ENSGAGP00000010840.1"/>
        <property type="gene ID" value="ENSGAGG00000008427.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070004">
        <property type="term" value="F:cysteine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.60.10:FF:000001">
        <property type="entry name" value="Secernin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.60.10">
        <property type="entry name" value="Penicillin V Acylase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005322">
        <property type="entry name" value="Peptidase_C69"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12994">
        <property type="entry name" value="SECERNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12994:SF7">
        <property type="entry name" value="SECERNIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03577">
        <property type="entry name" value="Peptidase_C69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="414" mass="46117" checksum="1AA2009A04A31DE4" modified="2019-05-08" version="1">MAVAPPSYCFVAFPPRTKDGLVVFGKNSARPREEVQEVVYFAAAAHDPGSKVECTYIAIDQVPKTHAIMLSRPAWLWGAEMGANEHGVCIANEAITTREPASETEALLGMDLVRLGLERGATAKEALDAIVSLLEEHGEGGNYYEDGSSCQAFQSAFLIVDRAEAWVLETVGKYWAAEKITEGVKCICNQLSLTTKIDAEHPELRSYAETQGWWTGDTEFNFSEVFSLAEDHLSCCSGQESLEKQEGNITVQTMMDVLRDKASGVCVDSETFLTTASIVSVLPQKPSSPCIHYFTGTPDPSRSIFKPFIFVDDVKLVPKVQSPFLGDDDPVKKKPRFQERPDRRHELYKAHEQARSVTENDQDKGQKLMRIMLDLERQGLEAMEDILTSTEVLHPAEVVDLFYDCVDTEIKFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452H809</accession>
    <name>A0A452H809_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452H809"/>
    <dbReference type="Ensembl" id="ENSGAGT00000012267.1">
        <property type="protein sequence ID" value="ENSGAGP00000010697.1"/>
        <property type="gene ID" value="ENSGAGG00000008349.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032727">
        <property type="entry name" value="CLAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28457">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28457:SF2">
        <property type="entry name" value="SIMILAR TO 4930578I06RIK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000010697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="209" mass="24130" checksum="59DDDECCAB03AE14" modified="2019-05-08" version="1">KSSKRVSLPKHCENPFKPCVLYEYFYHTLIRHYQLYQFVLCRERDIDQTSAHLEICVPPQPLPLTAGVNAEVWHYQQQLAALSAAEVQKRTNMLLLRETLHLEREHMLQRAYHDLKSPLQPGPSPSTGRGRLFWASGSQPLIGTKVIGTQIQALREILQTEIQTTFEILDLRLQKRALILNPPVPYPPPPLLEERAKKSTKAQLQKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HAW7</accession>
    <name>A0A452HAW7_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HAW7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000011875"/>
    <dbReference type="Ensembl" id="ENSGAGT00000013594.1">
        <property type="protein sequence ID" value="ENSGAGP00000011875.1"/>
        <property type="gene ID" value="ENSGAGG00000009140.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000180">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily C, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052606">
        <property type="entry name" value="DnaJ_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23082">
        <property type="entry name" value="Myb_DNA-binding_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="11">
        <location>
            <begin position="26"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="12">
        <location>
            <begin position="463"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="13">
        <location>
            <begin position="466"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="435"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="456"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000011875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="528" mass="61814" checksum="79A00EAE98B17EA9" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HBV8</accession>
    <name>A0A452HBV8_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">E3 ubiquitin-protein ligase CBL</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A452HBV8"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012249"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014029.1">
        <property type="protein sequence ID" value="ENSGAGP00000012249.1"/>
        <property type="gene ID" value="ENSGAGG00000009424.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000022">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.20:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024162">
        <property type="entry name" value="Adaptor_Cbl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014741">
        <property type="entry name" value="Adaptor_Cbl_EF_hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003153">
        <property type="entry name" value="Adaptor_Cbl_N_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024159">
        <property type="entry name" value="Cbl_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23007">
        <property type="entry name" value="CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23007:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CBL-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02262">
        <property type="entry name" value="Cbl_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02761">
        <property type="entry name" value="Cbl_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47668">
        <property type="entry name" value="N-terminal domain of cbl (N-cbl)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51506">
        <property type="entry name" value="CBL_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Cbl-PTB" evidence="7">
        <location>
            <begin position="35"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="256" mass="29647" checksum="3B889B9D651CE013" modified="2019-05-08" version="1">MANSLNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNSPPYILDILPDTYQHLRLILGNCDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDRRNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEIHQISSGLEAMALKSTIDLTCNDYISIFEFDIFTRLFQVRFLKYSALWKNLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HC60</accession>
    <name>A0A452HC60_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with PTBP2; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HC60"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014148.1">
        <property type="protein sequence ID" value="ENSGAGP00000012359.1"/>
        <property type="gene ID" value="ENSGAGG00000009498.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22435">
        <property type="entry name" value="KH-I_NOVA_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22436">
        <property type="entry name" value="KH-I_NOVA_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09031">
        <property type="entry name" value="KH-I_NOVA_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000019">
        <property type="entry name" value="RNA-binding protein Nova-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000020">
        <property type="entry name" value="RNA-binding protein Nova-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000022">
        <property type="entry name" value="RNA-binding protein Nova-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047275">
        <property type="entry name" value="KH-I_NOVA_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047276">
        <property type="entry name" value="KH-I_NOVA_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047274">
        <property type="entry name" value="KH-I_NOVA_rpt3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="10">
        <location>
            <begin position="18"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="10">
        <location>
            <begin position="116"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="10">
        <location>
            <begin position="370"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="457" mass="46630" checksum="FB811184E5B16339" modified="2019-05-08" version="1">MPELSVFPHEGQNANKEGEYFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGTTERVCLVQGTAEALNAVHNFIAEKVREIPQSVVKPESVNILQPQTTMNPDRAKQAKLIVPNSTAGLIIGKGGATVKAIMEQSGAWVQLSQKPEGINLQERVVTVSGEPEQIRKAVDIIVQKIQEDPQSSSCLNISYANITGPVANSNPTGSPYANSTDVLPAAAAAATASGLLGHTSLAGVGAFPAALPGFSGSDLLAISTALNTLASYGYNTNSLGLGLNSAAASGVLAAVAAGANPAAAAAANLLASYASDASASTSTPAGAVGAFTLGSLAAATGAANGYLGTASPLVASSFLATEKLVESAKDMVEIAVPENLVGAILGKGGKTLVEYQELTGARIQISKKGEFIPGTRNRKVTITGTPAATQAAQYLISQRVTYEQGVRATNPQKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HDE5</accession>
    <name>A0A452HDE5_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutathione transferase</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Theta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A452HDE5"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000012817"/>
    <dbReference type="Ensembl" id="ENSGAGT00000014691.1">
        <property type="protein sequence ID" value="ENSGAGP00000012817.1"/>
        <property type="gene ID" value="ENSGAGG00000009838.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSGAGT00000014695.1">
        <property type="protein sequence ID" value="ENSGAGP00000012821.1"/>
        <property type="gene ID" value="ENSGAGG00000009838.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03183">
        <property type="entry name" value="GST_C_Theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03050">
        <property type="entry name" value="GST_N_Theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000008">
        <property type="entry name" value="Glutathione S-transferase theta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000548">
        <property type="entry name" value="Glutathione S-transferase theta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040077">
        <property type="entry name" value="GST_C_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040075">
        <property type="entry name" value="GST_N_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051369">
        <property type="entry name" value="GST_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917:SF15">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE THETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="8">
        <location>
            <begin position="10"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="9">
        <location>
            <begin position="116"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000012821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="270" mass="30906" checksum="B27E974DFAB5E185" modified="2019-05-08" version="1">MFILKNAGRMGLELYLDLLSQPCRAVYIFAKKNNIPFEFKHVELFKDSILGMKMPGGNNPGHSHEGQHCRGEFGKMNILKKVPALKDGVLTLAESTAILLYLSWKYNTPDHWYPSDTQKRAQVDEYLSWHHTNIRGNAGKALWIKVLIPLFTGQTLPPEKLQEVMDELAISLKQFEEKFLQDKPFIVGSEISLADLMAIVELMQPIGVGCDIFEDRPKLSEWRNQVEEAVGKELFQEAHELILNIKELSNIQIDPQLKEHLAPVLRKMMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HE91</accession>
    <name>A0A452HE91_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HE91"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013122"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015026.1">
        <property type="protein sequence ID" value="ENSGAGP00000013122.1"/>
        <property type="gene ID" value="ENSGAGG00000010062.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001706">
        <property type="term" value="P:endoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002244">
        <property type="term" value="P:hematopoietic progenitor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000312">
        <property type="entry name" value="Mix-type homeobox gene 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042917">
        <property type="entry name" value="MIXL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47656">
        <property type="entry name" value="HOMEOBOX PROTEIN MIXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47656:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN MIXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="43"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="45"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="95"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="235" mass="25586" checksum="C95CD9274F8D4D02" modified="2019-05-08" version="1">RGLAAGGGCPSLSQALSPPASATVAAALPAPFPRDAAELVPSISQRRKRTNFTTAQLETLELVFNNTMYPDIYLREKLADMTQIPESRIQVWFQNRRAKSRRQRGKPCSSPAAAEPFPSHKAPAKNAHPAVGIQQHTSTTEPHHYYQAPGGPSGVAYATQPHFPSNGPSTSVKALEDQQPHYALSGVAQIIQIKEENAGAFQQWKKTSRITLLLLHSPPKSYLGLRQGWPNLLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HEM3</accession>
    <name>A0A452HEM3_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD repeat domain-containing protein 83</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mitogen-activated protein kinase organizer 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat MORG1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HEM3"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000013270"/>
    <dbReference type="Ensembl" id="ENSGAGT00000015191.1">
        <property type="protein sequence ID" value="ENSGAGP00000013270.1"/>
        <property type="gene ID" value="ENSGAGG00000010168.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000273">
        <property type="entry name" value="WD repeat domain-containing protein 83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051980">
        <property type="entry name" value="WD_repeat_MORG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842:SF3">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING PROTEIN 83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="134"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="176"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="218"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000013270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="430" mass="46182" checksum="409A31028D5E9939" modified="2019-05-08" version="1">MLLLDMLPGGADRCNTPRPGAMVPENPLSPRLAGASRSHTGRAGDEAGPTKSPAPLPSAAGPGSEEAGQGGRNQGSQNLRGRGTEWEPDTEAPLPSPRSQSPDLSDLATVGPIMAFPEPKPKKPELPKKLVQNLECKQGAVRAVRFNVDGNYCLTCGSDKSLKLWNPHKGTLLKTYSGHGYEVLDAAGSFNNSQLCSCGADKTVALWDVASGQVIRKFRGHAGKVNCVQFNEEATVILSGSIDSSVRCWDCRSRRPDPIQILDEAKDGVSSLKVSDYEILSGSVDGRVRRYDLRAGELYSDYIGSPVTSVCFSKDGQCTLAASLDSTLRLLDKDTGELLGEYTGHKNTAYKLDCCLSEKDTHVASCSEDGKVYFWDLVEGSLTLSLPVGRGVVQSLSFHPSEPGLLTATEGRVQFWREETHTAEEQPPAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGN7</accession>
    <name>A0A452HGN7_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Macoilin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Transmembrane protein 57</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the regulation of neuronal activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Rough endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the macoilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A452HGN7"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014032"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016060.1">
        <property type="protein sequence ID" value="ENSGAGP00000014032.1"/>
        <property type="gene ID" value="ENSGAGG00000010689.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030867">
        <property type="term" value="C:rough endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023041">
        <property type="term" value="P:neuronal signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019130">
        <property type="entry name" value="Macoilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47464">
        <property type="entry name" value="MACOILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47464:SF2">
        <property type="entry name" value="MACOILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09726">
        <property type="entry name" value="Macoilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="74"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="319"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="382"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="319"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="600" mass="69314" checksum="995A30EFB40E7C6E" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HGN9</accession>
    <name>A0A452HGN9_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rho GTPase activating protein 20</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HGN9"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014025"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016049.1">
        <property type="protein sequence ID" value="ENSGAGP00000014025.1"/>
        <property type="gene ID" value="ENSGAGG00000010678.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17115">
        <property type="entry name" value="RA_RHG20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04402">
        <property type="entry name" value="RhoGAP_ARHGAP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000025">
        <property type="entry name" value="Rho GTPase-activating protein 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047886">
        <property type="entry name" value="ARHGAP20-like_RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047887">
        <property type="entry name" value="ARHGAP20_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047888">
        <property type="entry name" value="ARHGAP20_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23179:SF36">
        <property type="entry name" value="RHO-GAP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23179">
        <property type="entry name" value="T-CELL ACTIVATION RHO GTPASE ACTIVATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22286">
        <property type="entry name" value="RHG20_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="4">
        <location>
            <begin position="61"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="5">
        <location>
            <begin position="217"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="587"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="769"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="587"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="773"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="787"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="841"/>
            <end position="860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1005" mass="111732" checksum="D5742748D17F4CF0" modified="2019-05-08" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HH41</accession>
    <name>A0A452HH41_9SAUR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A452HH41"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014184"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016234.1">
        <property type="protein sequence ID" value="ENSGAGP00000014184.1"/>
        <property type="gene ID" value="ENSGAGG00000010798.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050776">
        <property type="entry name" value="Ank_Repeat/CDKN_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201:SF0">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="71"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSGAGP00000014184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000291020"/>
        </source>
    </evidence>
    <sequence length="237" mass="25051" checksum="3D9079BADBB17C85" modified="2019-05-08" version="1">GPSGPVSESARCRARPPVCDSLCPPREPIAAAARPAPSRLPVTPAASRCQSDGLSLLLEAGTGVNAPADAFGQSPAHLAACGGQAFFLLWQLQTGANLNQQDCHGEAPIHKAAKVGSLECLALLVASDARIDLCNNDGQTAEDLAWAFGFLECAKFLTTAKHTQNMKLKEQSSCSLKDSCGLPREASSGQKRACGIMGPTNRKRRRSDESTRLEKTVLDYILVHTSKKALLIGLSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-05-08" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A452HIL0</accession>
    <name>A0A452HIL0_9SAUR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">carbonyl reductase (NADPH)</fullName>
            <ecNumber evidence="7">1.1.1.184</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Gopherus agassizii</name>
        <name type="common">Agassiz's desert tortoise</name>
        <dbReference type="NCBI Taxonomy" id="38772"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Archelosauria</taxon>
            <taxon>Testudinata</taxon>
            <taxon>Testudines</taxon>
            <taxon>Cryptodira</taxon>
            <taxon>Durocryptodira</taxon>
            <taxon>Testudinoidea</taxon>
            <taxon>Testudinidae</taxon>
            <taxon>Gopherus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2017" name="PLoS ONE" volume="12" first="e0177708" last="e0177708">
            <title>The Agassiz's desert tortoise genome provides a resource for the conservation of a threatened species.</title>
            <authorList>
                <person name="Tollis M."/>
                <person name="DeNardo D.F."/>
                <person name="Cornelius J.A."/>
                <person name="Dolby G.A."/>
                <person name="Edwards T."/>
                <person name="Henen B.T."/>
                <person name="Karl A.E."/>
                <person name="Murphy R.W."/>
                <person name="Kusumi K."/>
            </authorList>
            <dbReference type="PubMed" id="28562605"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.184" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A452HIL0"/>
    <dbReference type="STRING" id="38772.ENSGAGP00000014742"/>
    <dbReference type="Ensembl" id="ENSGAGT00000016850.1">
        <property type="protein sequence ID" value="ENSGAGP00000014742.1"/>
        <property type="gene ID" value="ENSGAGG00000011177.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000291020">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004090">
        <property type="term" value="F:carbonyl reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05324">
        <property type="entry name" value="carb_red_PTCR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000164">
        <property type="entry name" value="Carbonyl reductase [NADPH] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045313">
        <property type="entry name" value="CBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43963:SF4">
        <property type="entry name" value="CARBONYL REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43963"